CHRNE cDNA ORF clone, Canis lupus familiaris(dog)

The following CHRNE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe288084 XM_022418460.1
Latest version!
Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe288085 XM_022418461.1
Latest version!
Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe288086 XM_022418463.1
Latest version!
Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe288087 XM_022418464.1
Latest version!
Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe288088 XM_022418465.1
Latest version!
Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe288089 XM_022418466.1
Latest version!
Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe288090 XM_022418467.1
Latest version!
Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe288091 XM_014113501.2
Latest version!
Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe288092 XM_014113502.2
Latest version!
Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe36390 XM_014113500.1
Latest version!
Canis lupus familiaris cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe36391 XM_014113501.1
Latest version!
Canis lupus familiaris cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe36392 XM_014113502.1
Latest version!
Canis lupus familiaris cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $258.30
$369.00
OCe36393 XM_014113503.1
Latest version!
Canis lupus familiaris cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $258.30
$369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe288084
    Clone ID Related Accession (Same CDS sequence) XM_022418460.1
    Accession Version XM_022418460.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1698bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product acetylcholine receptor subunit epsilon isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGGTAAG ATGCCCCCAC
    CCGCACCCAC CGTCCTGGGG GCTAGTTCCG ACCTCCACCA CCCTGACTCC GGCTCGTCTC
    CAGAACGAGA AGGAGGAGAC CCTGACCACC AGCGTCTGGA TTGGAATCGA CTGGCAGGAT
    TACCGACTCA ACTACAGCAA GGACGACTTC GGGGGCGTGG AGACCTTGCG GGTCCCTTCG
    GAGCTCGTAT GGCTCCCGGA GATTGTGCTG GAGAACAACA TTGACGGCCA GTTCGGCGTG
    GCCTACGACG CCAACGTGCT GATCTACGAG GGCGGCTACG TGAGCTGGCT GCCCCCCGCC
    ATCTACCGCA GCACCTGCGC CGTGGAGGTC ACCTACTTCC CCTTCGACTG GCAGAACTGC
    TCGCTGGTCT TCCGCTCGCA GACGTACAAT GCCGAGGAGG TGGACTTCGT CTTCGCCGTG
    GACGACGACG GCGAGACCAT CAGCAAAATC GACATCGACA CCGAGGCCTA CACGGAGAAC
    GGCGAGTGGG CCATCGACTT CTGCCCCGGG CTGATCCGTC GCCCCAACGG AGCCTCCGCT
    GGGGACCCGG GGGTCGTCGA CATCATCTAC ACGCTCATCA TCCGCCGGAA GCCGCTCTTC
    TACGTCATCA ACATCATCGT GCCCTGCGTG CTCATCTCGG GCCTGGTGCT GCTCGCCTAC
    TTCCTGCCCG CGCAGGGTAA GGGGCCGCGG GACCCTGAGC CCGGCTCGCT GCTGGCGGGG
    CGGCTCTCAC CGGGCGCTCC CTCCAGCCGG CGGCCAGAAG TGCACCGTTT CCATCAACGT
    CCTACTCGCC CAGACCGTCT TCCTGTTCCT GATCGCTCAG AAAATCCCCG AGACGTCGCT
    GAGCGTGCCG ATGCTGGGCA GGTACCTCAT CTTCGTCATG GTGGTTGCCA CGATCATCGT
    CATGAACTGT GTCATCGTGC TCAACGTGTC TCTGCGGACG CCCACCACAC ACGCCATGTC
    CCCGCGGCTG CGCCACGTCC TGCTGGAGCA GCTGCCCAGC CTCCTGGGCT CCAGCGCACC
    CCCCGAGGCT CCCCGGGCCG CCCCGCCGCC CAGGCGCGCG TCCTCGGTGG GCCTGCTGCT
    CCGTGCGGAG GAGCTGATCC TGAAAAAGCC GCGGAGCGAG CTCGTATTCG AGGGGCAGAG
    GCACCGGCAC GGGGCCTGGA CTGCAGCTGC CCTCTGCCAG AGCCTGGGCG CCGCCGCCCC
    CGAGATCCGC TGCTGCGTGG AGGCTGTGAA CTTCGTGGCC GAGAGCACAC GGAGCCAGGA
    GGCCTCCGTC GAGGAGGTGT CCGACTGGGT GCGCATGGGG AAGGCCCTGG ATCACCTCTG
    CTTCTGGGCC GCCTTGGTGC TGTTCGGCGT GGGGTCCAGC GTCATTTTCC TTGGGGGCTA
    CTTCAACCAA GTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274168.1
    CDS322..2019
    Translation

    Target ORF information:

    RefSeq Version XM_022418460.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418460.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGGTAAG ATGCCCCCAC
    CCGCACCCAC CGTCCTGGGG GCTAGTTCCG ACCTCCACCA CCCTGACTCC GGCTCGTCTC
    CAGAACGAGA AGGAGGAGAC CCTGACCACC AGCGTCTGGA TTGGAATCGA CTGGCAGGAT
    TACCGACTCA ACTACAGCAA GGACGACTTC GGGGGCGTGG AGACCTTGCG GGTCCCTTCG
    GAGCTCGTAT GGCTCCCGGA GATTGTGCTG GAGAACAACA TTGACGGCCA GTTCGGCGTG
    GCCTACGACG CCAACGTGCT GATCTACGAG GGCGGCTACG TGAGCTGGCT GCCCCCCGCC
    ATCTACCGCA GCACCTGCGC CGTGGAGGTC ACCTACTTCC CCTTCGACTG GCAGAACTGC
    TCGCTGGTCT TCCGCTCGCA GACGTACAAT GCCGAGGAGG TGGACTTCGT CTTCGCCGTG
    GACGACGACG GCGAGACCAT CAGCAAAATC GACATCGACA CCGAGGCCTA CACGGAGAAC
    GGCGAGTGGG CCATCGACTT CTGCCCCGGG CTGATCCGTC GCCCCAACGG AGCCTCCGCT
    GGGGACCCGG GGGTCGTCGA CATCATCTAC ACGCTCATCA TCCGCCGGAA GCCGCTCTTC
    TACGTCATCA ACATCATCGT GCCCTGCGTG CTCATCTCGG GCCTGGTGCT GCTCGCCTAC
    TTCCTGCCCG CGCAGGGTAA GGGGCCGCGG GACCCTGAGC CCGGCTCGCT GCTGGCGGGG
    CGGCTCTCAC CGGGCGCTCC CTCCAGCCGG CGGCCAGAAG TGCACCGTTT CCATCAACGT
    CCTACTCGCC CAGACCGTCT TCCTGTTCCT GATCGCTCAG AAAATCCCCG AGACGTCGCT
    GAGCGTGCCG ATGCTGGGCA GGTACCTCAT CTTCGTCATG GTGGTTGCCA CGATCATCGT
    CATGAACTGT GTCATCGTGC TCAACGTGTC TCTGCGGACG CCCACCACAC ACGCCATGTC
    CCCGCGGCTG CGCCACGTCC TGCTGGAGCA GCTGCCCAGC CTCCTGGGCT CCAGCGCACC
    CCCCGAGGCT CCCCGGGCCG CCCCGCCGCC CAGGCGCGCG TCCTCGGTGG GCCTGCTGCT
    CCGTGCGGAG GAGCTGATCC TGAAAAAGCC GCGGAGCGAG CTCGTATTCG AGGGGCAGAG
    GCACCGGCAC GGGGCCTGGA CTGCAGCTGC CCTCTGCCAG AGCCTGGGCG CCGCCGCCCC
    CGAGATCCGC TGCTGCGTGG AGGCTGTGAA CTTCGTGGCC GAGAGCACAC GGAGCCAGGA
    GGCCTCCGTC GAGGAGGTGT CCGACTGGGT GCGCATGGGG AAGGCCCTGG ATCACCTCTG
    CTTCTGGGCC GCCTTGGTGC TGTTCGGCGT GGGGTCCAGC GTCATTTTCC TTGGGGGCTA
    CTTCAACCAA GTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288085
    Clone ID Related Accession (Same CDS sequence) XM_022418461.1
    Accession Version XM_022418461.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product acetylcholine receptor subunit epsilon isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGGTAAG ATGCCCCCAC
    CCGCACCCAC CGTCCTGGGG GCTAGTTCCG ACCTCCACCA CCCTGACTCC GGCTCGTCTC
    CAGAACGAGA AGGAGGAGAC CCTGACCACC AGCGTCTGGA TTGGAATCGA CTGGCAGGAT
    TACCGACTCA ACTACAGCAA GGACGACTTC GGGGGCGTGG AGACCTTGCG GGTCCCTTCG
    GAGCTCGTAT GGCTCCCGGA GATTGTGCTG GAGAACAACA TTGACGGCCA GTTCGGCGTG
    GCCTACGACG CCAACGTGCT GATCTACGAG GGCGGCTACG TGAGCTGGCT GCCCCCCGCC
    ATCTACCGCA GCACCTGCGC CGTGGAGGTC ACCTACTTCC CCTTCGACTG GCAGAACTGC
    TCGCTGGTCT TCCGCTCGCA GACGTACAAT GCCGAGGAGG TGGACTTCGT CTTCGCCGTG
    GACGACGACG GCGAGACCAT CAGCAAAATC GACATCGACA CCGAGGCCTA CACGGAGAAC
    GGCGAGTGGG CCATCGACTT CTGCCCCGGG CTGATCCGTC GCCCCAACGG AGCCTCCGCT
    GGGGACCCGG GGGTCGTCGA CATCATCTAC ACGCTCATCA TCCGCCGGAA GCCGCTCTTC
    TACGTCATCA ACATCATCGT GCCCTGCGTG CTCATCTCGG GCCTGGTGCT GCTCGCCTAC
    TTCCTGCCCG CGCAGGGTAA GGGGCCGCGG GACCCTGAGC CCGGCTCGCT GCTGGCGGGG
    CGGCTCTCAC CGGGCGCTCC CTCCAGCCGG CGGCCAGAAG TGCACCGTTT CCATCAACGT
    CCTACTCGCC CAGACCGTCT TCCTGTTCCT GATCGCTCAG AAAATCCCCG AGACGTCGCT
    GAGCGTGCCG ATGCTGGGCA GGTACCTCAT CTTCGTCATG GTGGTTGCCA CGATCATCGT
    CATGAACTGT GTCATCGTGC TCAACGTGTC TCTGCGGACG CCCACCACAC ACGCCATGTC
    CCCGCGGCTG CGCCACGTCC TGCTGGAGCA GCTGCCCAGC CTCCTGGGCT CCAGCGCACC
    CCCCGAGGCT CCCCGGGCCG CCCCGCCGCC CAGGCGCGCG TCCTCGGTGG GCCTGCTGCT
    CCGTGCGGAG GAGCTGATCC TGAAAAAGCC GCGGAGCGAG CTCGTATTCG AGGGGCAGAG
    GCACCGGCAC GGGGCCTGGA CTGCTGCCCT CTGCCAGAGC CTGGGCGCCG CCGCCCCCGA
    GATCCGCTGC TGCGTGGAGG CTGTGAACTT CGTGGCCGAG AGCACACGGA GCCAGGAGGC
    CTCCGTCGAG GAGGTGTCCG ACTGGGTGCG CATGGGGAAG GCCCTGGATC ACCTCTGCTT
    CTGGGCCGCC TTGGTGCTGT TCGGCGTGGG GTCCAGCGTC ATTTTCCTTG GGGGCTACTT
    CAACCAAGTG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274169.1
    CDS319..2013
    Translation

    Target ORF information:

    RefSeq Version XM_022418461.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418461.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGGTAAG ATGCCCCCAC
    CCGCACCCAC CGTCCTGGGG GCTAGTTCCG ACCTCCACCA CCCTGACTCC GGCTCGTCTC
    CAGAACGAGA AGGAGGAGAC CCTGACCACC AGCGTCTGGA TTGGAATCGA CTGGCAGGAT
    TACCGACTCA ACTACAGCAA GGACGACTTC GGGGGCGTGG AGACCTTGCG GGTCCCTTCG
    GAGCTCGTAT GGCTCCCGGA GATTGTGCTG GAGAACAACA TTGACGGCCA GTTCGGCGTG
    GCCTACGACG CCAACGTGCT GATCTACGAG GGCGGCTACG TGAGCTGGCT GCCCCCCGCC
    ATCTACCGCA GCACCTGCGC CGTGGAGGTC ACCTACTTCC CCTTCGACTG GCAGAACTGC
    TCGCTGGTCT TCCGCTCGCA GACGTACAAT GCCGAGGAGG TGGACTTCGT CTTCGCCGTG
    GACGACGACG GCGAGACCAT CAGCAAAATC GACATCGACA CCGAGGCCTA CACGGAGAAC
    GGCGAGTGGG CCATCGACTT CTGCCCCGGG CTGATCCGTC GCCCCAACGG AGCCTCCGCT
    GGGGACCCGG GGGTCGTCGA CATCATCTAC ACGCTCATCA TCCGCCGGAA GCCGCTCTTC
    TACGTCATCA ACATCATCGT GCCCTGCGTG CTCATCTCGG GCCTGGTGCT GCTCGCCTAC
    TTCCTGCCCG CGCAGGGTAA GGGGCCGCGG GACCCTGAGC CCGGCTCGCT GCTGGCGGGG
    CGGCTCTCAC CGGGCGCTCC CTCCAGCCGG CGGCCAGAAG TGCACCGTTT CCATCAACGT
    CCTACTCGCC CAGACCGTCT TCCTGTTCCT GATCGCTCAG AAAATCCCCG AGACGTCGCT
    GAGCGTGCCG ATGCTGGGCA GGTACCTCAT CTTCGTCATG GTGGTTGCCA CGATCATCGT
    CATGAACTGT GTCATCGTGC TCAACGTGTC TCTGCGGACG CCCACCACAC ACGCCATGTC
    CCCGCGGCTG CGCCACGTCC TGCTGGAGCA GCTGCCCAGC CTCCTGGGCT CCAGCGCACC
    CCCCGAGGCT CCCCGGGCCG CCCCGCCGCC CAGGCGCGCG TCCTCGGTGG GCCTGCTGCT
    CCGTGCGGAG GAGCTGATCC TGAAAAAGCC GCGGAGCGAG CTCGTATTCG AGGGGCAGAG
    GCACCGGCAC GGGGCCTGGA CTGCTGCCCT CTGCCAGAGC CTGGGCGCCG CCGCCCCCGA
    GATCCGCTGC TGCGTGGAGG CTGTGAACTT CGTGGCCGAG AGCACACGGA GCCAGGAGGC
    CTCCGTCGAG GAGGTGTCCG ACTGGGTGCG CATGGGGAAG GCCCTGGATC ACCTCTGCTT
    CTGGGCCGCC TTGGTGCTGT TCGGCGTGGG GTCCAGCGTC ATTTTCCTTG GGGGCTACTT
    CAACCAAGTG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288086
    Clone ID Related Accession (Same CDS sequence) XM_022418463.1
    Accession Version XM_022418463.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product acetylcholine receptor subunit epsilon isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTG TGGAGCTGAG GCCAAGAACG AGGAGCTGCA TCTGTACCAC
    CATCTCTTCG ACAACTATGA CCCAGGAAGC CGGCCCGTGA AGGAGCCTGA AGACACGGTC
    ACCATCACCC TCAAGGTCAC CCTGACCAAC CTTATCTCCC TGGTAAGATG CCCCCACCCG
    CACCCACCGT CCTGGGGGCT AGTTCCGACC TCCACCACCC TGACTCCGGC TCGTCTCCAG
    AACGAGAAGG AGGAGACCCT GACCACCAGC GTCTGGATTG GAATCGACTG GCAGGATTAC
    CGACTCAACT ACAGCAAGGA CGACTTCGGG GGCGTGGAGA CCTTGCGGGT CCCTTCGGAG
    CTCGTATGGC TCCCGGAGAT TGTGCTGGAG AACAACATTG ACGGCCAGTT CGGCGTGGCC
    TACGACGCCA ACGTGCTGAT CTACGAGGGC GGCTACGTGA GCTGGCTGCC CCCCGCCATC
    TACCGCAGCA CCTGCGCCGT GGAGGTCACC TACTTCCCCT TCGACTGGCA GAACTGCTCG
    CTGGTCTTCC GCTCGCAGAC GTACAATGCC GAGGAGGTGG ACTTCGTCTT CGCCGTGGAC
    GACGACGGCG AGACCATCAG CAAAATCGAC ATCGACACCG AGGCCTACAC GGAGAACGGC
    GAGTGGGCCA TCGACTTCTG CCCCGGGCTG ATCCGTCGCC CCAACGGAGC CTCCGCTGGG
    GACCCGGGGG TCGTCGACAT CATCTACACG CTCATCATCC GCCGGAAGCC GCTCTTCTAC
    GTCATCAACA TCATCGTGCC CTGCGTGCTC ATCTCGGGCC TGGTGCTGCT CGCCTACTTC
    CTGCCCGCGC AGGGTAAGGG GCCGCGGGAC CCTGAGCCCG GCTCGCTGCT GGCGGGGCGG
    CTCTCACCGG GCGCTCCCTC CAGCCGGCGG CCAGAAGTGC ACCGTTTCCA TCAACGTCCT
    ACTCGCCCAG ACCGTCTTCC TGTTCCTGAT CGCTCAGAAA ATCCCCGAGA CGTCGCTGAG
    CGTGCCGATG CTGGGCAGGT ACCTCATCTT CGTCATGGTG GTTGCCACGA TCATCGTCAT
    GAACTGTGTC ATCGTGCTCA ACGTGTCTCT GCGGACGCCC ACCACACACG CCATGTCCCC
    GCGGCTGCGC CACGTCCTGC TGGAGCAGCT GCCCAGCCTC CTGGGCTCCA GCGCACCCCC
    CGAGGCTCCC CGGGCCGCCC CGCCGCCCAG GCGCGCGTCC TCGGTGGGCC TGCTGCTCCG
    TGCGGAGGAG CTGATCCTGA AAAAGCCGCG GAGCGAGCTC GTATTCGAGG GGCAGAGGCA
    CCGGCACGGG GCCTGGACTG CAGCTGCCCT CTGCCAGAGC CTGGGCGCCG CCGCCCCCGA
    GATCCGCTGC TGCGTGGAGG CTGTGAACTT CGTGGCCGAG AGCACACGGA GCCAGGAGGC
    CTCCGTCGAG GAGGTGTCCG ACTGGGTGCG CATGGGGAAG GCCCTGGATC ACCTCTGCTT
    CTGGGCCGCC TTGGTGCTGT TCGGCGTGGG GTCCAGCGTC ATTTTCCTTG GGGGCTACTT
    CAACCAAGTG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274171.1
    CDS322..2016
    Translation

    Target ORF information:

    RefSeq Version XM_022418463.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418463.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTG TGGAGCTGAG GCCAAGAACG AGGAGCTGCA TCTGTACCAC
    CATCTCTTCG ACAACTATGA CCCAGGAAGC CGGCCCGTGA AGGAGCCTGA AGACACGGTC
    ACCATCACCC TCAAGGTCAC CCTGACCAAC CTTATCTCCC TGGTAAGATG CCCCCACCCG
    CACCCACCGT CCTGGGGGCT AGTTCCGACC TCCACCACCC TGACTCCGGC TCGTCTCCAG
    AACGAGAAGG AGGAGACCCT GACCACCAGC GTCTGGATTG GAATCGACTG GCAGGATTAC
    CGACTCAACT ACAGCAAGGA CGACTTCGGG GGCGTGGAGA CCTTGCGGGT CCCTTCGGAG
    CTCGTATGGC TCCCGGAGAT TGTGCTGGAG AACAACATTG ACGGCCAGTT CGGCGTGGCC
    TACGACGCCA ACGTGCTGAT CTACGAGGGC GGCTACGTGA GCTGGCTGCC CCCCGCCATC
    TACCGCAGCA CCTGCGCCGT GGAGGTCACC TACTTCCCCT TCGACTGGCA GAACTGCTCG
    CTGGTCTTCC GCTCGCAGAC GTACAATGCC GAGGAGGTGG ACTTCGTCTT CGCCGTGGAC
    GACGACGGCG AGACCATCAG CAAAATCGAC ATCGACACCG AGGCCTACAC GGAGAACGGC
    GAGTGGGCCA TCGACTTCTG CCCCGGGCTG ATCCGTCGCC CCAACGGAGC CTCCGCTGGG
    GACCCGGGGG TCGTCGACAT CATCTACACG CTCATCATCC GCCGGAAGCC GCTCTTCTAC
    GTCATCAACA TCATCGTGCC CTGCGTGCTC ATCTCGGGCC TGGTGCTGCT CGCCTACTTC
    CTGCCCGCGC AGGGTAAGGG GCCGCGGGAC CCTGAGCCCG GCTCGCTGCT GGCGGGGCGG
    CTCTCACCGG GCGCTCCCTC CAGCCGGCGG CCAGAAGTGC ACCGTTTCCA TCAACGTCCT
    ACTCGCCCAG ACCGTCTTCC TGTTCCTGAT CGCTCAGAAA ATCCCCGAGA CGTCGCTGAG
    CGTGCCGATG CTGGGCAGGT ACCTCATCTT CGTCATGGTG GTTGCCACGA TCATCGTCAT
    GAACTGTGTC ATCGTGCTCA ACGTGTCTCT GCGGACGCCC ACCACACACG CCATGTCCCC
    GCGGCTGCGC CACGTCCTGC TGGAGCAGCT GCCCAGCCTC CTGGGCTCCA GCGCACCCCC
    CGAGGCTCCC CGGGCCGCCC CGCCGCCCAG GCGCGCGTCC TCGGTGGGCC TGCTGCTCCG
    TGCGGAGGAG CTGATCCTGA AAAAGCCGCG GAGCGAGCTC GTATTCGAGG GGCAGAGGCA
    CCGGCACGGG GCCTGGACTG CAGCTGCCCT CTGCCAGAGC CTGGGCGCCG CCGCCCCCGA
    GATCCGCTGC TGCGTGGAGG CTGTGAACTT CGTGGCCGAG AGCACACGGA GCCAGGAGGC
    CTCCGTCGAG GAGGTGTCCG ACTGGGTGCG CATGGGGAAG GCCCTGGATC ACCTCTGCTT
    CTGGGCCGCC TTGGTGCTGT TCGGCGTGGG GTCCAGCGTC ATTTTCCTTG GGGGCTACTT
    CAACCAAGTG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288087
    Clone ID Related Accession (Same CDS sequence) XM_022418464.1
    Accession Version XM_022418464.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1653bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product acetylcholine receptor subunit epsilon isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGGTAAG ATGCCCCCAC
    CCGCACCCAC CGTCCTGGGG GCTAGTTCCG ACCTCCACCA CCCTGACTCC GGCTCGTCTC
    CAGAACGAGA AGGAGGAGAC CCTGACCACC AGCGTCTGGA TTGGAATCGA CTGGCAGGAT
    TACCGACTCA ACTACAGCAA GGACGACTTC GGGGGCGTGG AGACCTTGCG GGTCCCTTCG
    GAGCTCGTAT GGCTCCCGGA GATTGTGCTG GAGAACAACA TTGACGGCCA GTTCGGCGTG
    GCCTACGACG CCAACGTGCT GATCTACGAG GGCGGCTACG TGAGCTGGCT GCCCCCCGCC
    ATCTACCGCA GCACCTGCGC CGTGGAGGTC ACCTACTTCC CCTTCGACTG GCAGAACTGC
    TCGCTGGTCT TCCGCTCGCA GACGTACAAT GCCGAGGAGG TGGACTTCGT CTTCGCCGTG
    GACGACGACG GCGAGACCAT CAGCAAAATC GACATCGACA CCGAGGCCTA CACGGAGAAC
    GGCGAGTGGG CCATCGACTT CTGCCCCGGG CTGATCCGTC GCCCCAACGG AGCCTCCGCT
    GGGGACCCGG GGGTCGTCGA CATCATCTAC ACGCTCATCA TCCGCCGGAA GCCGCTCTTC
    TACGTCATCA ACATCATCGT GCCCTGCGTG CTCATCTCGG GCCTGGTGCT GCTCGCCTAC
    TTCCTGCCCG CGCAGGCCGG CGGCCAGAAG TGCACCGTTT CCATCAACGT CCTACTCGCC
    CAGACCGTCT TCCTGTTCCT GATCGCTCAG AAAATCCCCG AGACGTCGCT GAGCGTGCCG
    ATGCTGGGCA GGTACCTCAT CTTCGTCATG GTGGTTGCCA CGATCATCGT CATGAACTGT
    GTCATCGTGC TCAACGTGTC TCTGCGGACG CCCACCACAC ACGCCATGTC CCCGCGGCTG
    CGCCACGTCC TGCTGGAGCA GCTGCCCAGC CTCCTGGGCT CCAGCGCACC CCCCGAGGCT
    CCCCGGGCCG CCCCGCCGCC CAGGCGCGCG TCCTCGGTGG GCCTGCTGCT CCGTGCGGAG
    GAGCTGATCC TGAAAAAGCC GCGGAGCGAG CTCGTATTCG AGGGGCAGAG GCACCGGCAC
    GGGGCCTGGA CTGCAGCTGC CCTCTGCCAG AGCCTGGGCG CCGCCGCCCC CGAGATCCGC
    TGCTGCGTGG AGGCTGTGAA CTTCGTGGCC GAGAGCACAC GGAGCCAGGA GGCCTCCGTC
    GAGGAGGTGT CCGACTGGGT GCGCATGGGG AAGGCCCTGG ATCACCTCTG CTTCTGGGCC
    GCCTTGGTGC TGTTCGGCGT GGGGTCCAGC GTCATTTTCC TTGGGGGCTA CTTCAACCAA
    GTGCCTGAGC TGCCCTACCC ACCGTGCATG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274172.1
    CDS320..1972
    Translation

    Target ORF information:

    RefSeq Version XM_022418464.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418464.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGGTAAG ATGCCCCCAC
    CCGCACCCAC CGTCCTGGGG GCTAGTTCCG ACCTCCACCA CCCTGACTCC GGCTCGTCTC
    CAGAACGAGA AGGAGGAGAC CCTGACCACC AGCGTCTGGA TTGGAATCGA CTGGCAGGAT
    TACCGACTCA ACTACAGCAA GGACGACTTC GGGGGCGTGG AGACCTTGCG GGTCCCTTCG
    GAGCTCGTAT GGCTCCCGGA GATTGTGCTG GAGAACAACA TTGACGGCCA GTTCGGCGTG
    GCCTACGACG CCAACGTGCT GATCTACGAG GGCGGCTACG TGAGCTGGCT GCCCCCCGCC
    ATCTACCGCA GCACCTGCGC CGTGGAGGTC ACCTACTTCC CCTTCGACTG GCAGAACTGC
    TCGCTGGTCT TCCGCTCGCA GACGTACAAT GCCGAGGAGG TGGACTTCGT CTTCGCCGTG
    GACGACGACG GCGAGACCAT CAGCAAAATC GACATCGACA CCGAGGCCTA CACGGAGAAC
    GGCGAGTGGG CCATCGACTT CTGCCCCGGG CTGATCCGTC GCCCCAACGG AGCCTCCGCT
    GGGGACCCGG GGGTCGTCGA CATCATCTAC ACGCTCATCA TCCGCCGGAA GCCGCTCTTC
    TACGTCATCA ACATCATCGT GCCCTGCGTG CTCATCTCGG GCCTGGTGCT GCTCGCCTAC
    TTCCTGCCCG CGCAGGCCGG CGGCCAGAAG TGCACCGTTT CCATCAACGT CCTACTCGCC
    CAGACCGTCT TCCTGTTCCT GATCGCTCAG AAAATCCCCG AGACGTCGCT GAGCGTGCCG
    ATGCTGGGCA GGTACCTCAT CTTCGTCATG GTGGTTGCCA CGATCATCGT CATGAACTGT
    GTCATCGTGC TCAACGTGTC TCTGCGGACG CCCACCACAC ACGCCATGTC CCCGCGGCTG
    CGCCACGTCC TGCTGGAGCA GCTGCCCAGC CTCCTGGGCT CCAGCGCACC CCCCGAGGCT
    CCCCGGGCCG CCCCGCCGCC CAGGCGCGCG TCCTCGGTGG GCCTGCTGCT CCGTGCGGAG
    GAGCTGATCC TGAAAAAGCC GCGGAGCGAG CTCGTATTCG AGGGGCAGAG GCACCGGCAC
    GGGGCCTGGA CTGCAGCTGC CCTCTGCCAG AGCCTGGGCG CCGCCGCCCC CGAGATCCGC
    TGCTGCGTGG AGGCTGTGAA CTTCGTGGCC GAGAGCACAC GGAGCCAGGA GGCCTCCGTC
    GAGGAGGTGT CCGACTGGGT GCGCATGGGG AAGGCCCTGG ATCACCTCTG CTTCTGGGCC
    GCCTTGGTGC TGTTCGGCGT GGGGTCCAGC GTCATTTTCC TTGGGGGCTA CTTCAACCAA
    GTGCCTGAGC TGCCCTACCC ACCGTGCATG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288088
    Clone ID Related Accession (Same CDS sequence) XM_022418465.1
    Accession Version XM_022418465.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product acetylcholine receptor subunit epsilon isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGGTAAG ATGCCCCCAC
    CCGCACCCAC CGTCCTGGGG GCTAGTTCCG ACCTCCACCA CCCTGACTCC GGCTCGTCTC
    CAGAACGAGA AGGAGGAGAC CCTGACCACC AGCGTCTGGA TTGGAATCGA CTGGCAGGAT
    TACCGACTCA ACTACAGCAA GGACGACTTC GGGGGCGTGG AGACCTTGCG GGTCCCTTCG
    GAGCTCGTAT GGCTCCCGGA GATTGTGCTG GAGAACAACA TTGACGGCCA GTTCGGCGTG
    GCCTACGACG CCAACGTGCT GATCTACGAG GGCGGCTACG TGAGCTGGCT GCCCCCCGCC
    ATCTACCGCA GCACCTGCGC CGTGGAGGTC ACCTACTTCC CCTTCGACTG GCAGAACTGC
    TCGCTGGTCT TCCGCTCGCA GACGTACAAT GCCGAGGAGG TGGACTTCGT CTTCGCCGTG
    GACGACGACG GCGAGACCAT CAGCAAAATC GACATCGACA CCGAGGCCTA CACGGAGAAC
    GGCGAGTGGG CCATCGACTT CTGCCCCGGG CTGATCCGTC GCCCCAACGG AGCCTCCGCT
    GGGGACCCGG GGGTCGTCGA CATCATCTAC ACGCTCATCA TCCGCCGGAA GCCGCTCTTC
    TACGTCATCA ACATCATCGT GCCCTGCGTG CTCATCTCGG GCCTGGTGCT GCTCGCCTAC
    TTCCTGCCCG CGCAGGCCGG CGGCCAGAAG TGCACCGTTT CCATCAACGT CCTACTCGCC
    CAGACCGTCT TCCTGTTCCT GATCGCTCAG AAAATCCCCG AGACGTCGCT GAGCGTGCCG
    ATGCTGGGCA GGTACCTCAT CTTCGTCATG GTGGTTGCCA CGATCATCGT CATGAACTGT
    GTCATCGTGC TCAACGTGTC TCTGCGGACG CCCACCACAC ACGCCATGTC CCCGCGGCTG
    CGCCACGTCC TGCTGGAGCA GCTGCCCAGC CTCCTGGGCT CCAGCGCACC CCCCGAGGCT
    CCCCGGGCCG CCCCGCCGCC CAGGCGCGCG TCCTCGGTGG GCCTGCTGCT CCGTGCGGAG
    GAGCTGATCC TGAAAAAGCC GCGGAGCGAG CTCGTATTCG AGGGGCAGAG GCACCGGCAC
    GGGGCCTGGA CTGCTGCCCT CTGCCAGAGC CTGGGCGCCG CCGCCCCCGA GATCCGCTGC
    TGCGTGGAGG CTGTGAACTT CGTGGCCGAG AGCACACGGA GCCAGGAGGC CTCCGTCGAG
    GAGGTGTCCG ACTGGGTGCG CATGGGGAAG GCCCTGGATC ACCTCTGCTT CTGGGCCGCC
    TTGGTGCTGT TCGGCGTGGG GTCCAGCGTC ATTTTCCTTG GGGGCTACTT CAACCAAGTG
    CCTGAGCTGC CCTACCCACC GTGCATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274173.1
    CDS317..1966
    Translation

    Target ORF information:

    RefSeq Version XM_022418465.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418465.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGGTAAG ATGCCCCCAC
    CCGCACCCAC CGTCCTGGGG GCTAGTTCCG ACCTCCACCA CCCTGACTCC GGCTCGTCTC
    CAGAACGAGA AGGAGGAGAC CCTGACCACC AGCGTCTGGA TTGGAATCGA CTGGCAGGAT
    TACCGACTCA ACTACAGCAA GGACGACTTC GGGGGCGTGG AGACCTTGCG GGTCCCTTCG
    GAGCTCGTAT GGCTCCCGGA GATTGTGCTG GAGAACAACA TTGACGGCCA GTTCGGCGTG
    GCCTACGACG CCAACGTGCT GATCTACGAG GGCGGCTACG TGAGCTGGCT GCCCCCCGCC
    ATCTACCGCA GCACCTGCGC CGTGGAGGTC ACCTACTTCC CCTTCGACTG GCAGAACTGC
    TCGCTGGTCT TCCGCTCGCA GACGTACAAT GCCGAGGAGG TGGACTTCGT CTTCGCCGTG
    GACGACGACG GCGAGACCAT CAGCAAAATC GACATCGACA CCGAGGCCTA CACGGAGAAC
    GGCGAGTGGG CCATCGACTT CTGCCCCGGG CTGATCCGTC GCCCCAACGG AGCCTCCGCT
    GGGGACCCGG GGGTCGTCGA CATCATCTAC ACGCTCATCA TCCGCCGGAA GCCGCTCTTC
    TACGTCATCA ACATCATCGT GCCCTGCGTG CTCATCTCGG GCCTGGTGCT GCTCGCCTAC
    TTCCTGCCCG CGCAGGCCGG CGGCCAGAAG TGCACCGTTT CCATCAACGT CCTACTCGCC
    CAGACCGTCT TCCTGTTCCT GATCGCTCAG AAAATCCCCG AGACGTCGCT GAGCGTGCCG
    ATGCTGGGCA GGTACCTCAT CTTCGTCATG GTGGTTGCCA CGATCATCGT CATGAACTGT
    GTCATCGTGC TCAACGTGTC TCTGCGGACG CCCACCACAC ACGCCATGTC CCCGCGGCTG
    CGCCACGTCC TGCTGGAGCA GCTGCCCAGC CTCCTGGGCT CCAGCGCACC CCCCGAGGCT
    CCCCGGGCCG CCCCGCCGCC CAGGCGCGCG TCCTCGGTGG GCCTGCTGCT CCGTGCGGAG
    GAGCTGATCC TGAAAAAGCC GCGGAGCGAG CTCGTATTCG AGGGGCAGAG GCACCGGCAC
    GGGGCCTGGA CTGCTGCCCT CTGCCAGAGC CTGGGCGCCG CCGCCCCCGA GATCCGCTGC
    TGCGTGGAGG CTGTGAACTT CGTGGCCGAG AGCACACGGA GCCAGGAGGC CTCCGTCGAG
    GAGGTGTCCG ACTGGGTGCG CATGGGGAAG GCCCTGGATC ACCTCTGCTT CTGGGCCGCC
    TTGGTGCTGT TCGGCGTGGG GTCCAGCGTC ATTTTCCTTG GGGGCTACTT CAACCAAGTG
    CCTGAGCTGC CCTACCCACC GTGCATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288089
    Clone ID Related Accession (Same CDS sequence) XM_022418466.1
    Accession Version XM_022418466.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1620bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product acetylcholine receptor subunit epsilon isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGAACGA GAAGGAGGAG
    ACCCTGACCA CCAGCGTCTG GATTGGAATC GACTGGCAGG ATTACCGACT CAACTACAGC
    AAGGACGACT TCGGGGGCGT GGAGACCTTG CGGGTCCCTT CGGAGCTCGT ATGGCTCCCG
    GAGATTGTGC TGGAGAACAA CATTGACGGC CAGTTCGGCG TGGCCTACGA CGCCAACGTG
    CTGATCTACG AGGGCGGCTA CGTGAGCTGG CTGCCCCCCG CCATCTACCG CAGCACCTGC
    GCCGTGGAGG TCACCTACTT CCCCTTCGAC TGGCAGAACT GCTCGCTGGT CTTCCGCTCG
    CAGACGTACA ATGCCGAGGA GGTGGACTTC GTCTTCGCCG TGGACGACGA CGGCGAGACC
    ATCAGCAAAA TCGACATCGA CACCGAGGCC TACACGGAGA ACGGCGAGTG GGCCATCGAC
    TTCTGCCCCG GGCTGATCCG TCGCCCCAAC GGAGCCTCCG CTGGGGACCC GGGGGTCGTC
    GACATCATCT ACACGCTCAT CATCCGCCGG AAGCCGCTCT TCTACGTCAT CAACATCATC
    GTGCCCTGCG TGCTCATCTC GGGCCTGGTG CTGCTCGCCT ACTTCCTGCC CGCGCAGGGT
    AAGGGGCCGC GGGACCCTGA GCCCGGCTCG CTGCTGGCGG GGCGGCTCTC ACCGGGCGCT
    CCCTCCAGCC GGCGGCCAGA AGTGCACCGT TTCCATCAAC GTCCTACTCG CCCAGACCGT
    CTTCCTGTTC CTGATCGCTC AGAAAATCCC CGAGACGTCG CTGAGCGTGC CGATGCTGGG
    CAGGTACCTC ATCTTCGTCA TGGTGGTTGC CACGATCATC GTCATGAACT GTGTCATCGT
    GCTCAACGTG TCTCTGCGGA CGCCCACCAC ACACGCCATG TCCCCGCGGC TGCGCCACGT
    CCTGCTGGAG CAGCTGCCCA GCCTCCTGGG CTCCAGCGCA CCCCCCGAGG CTCCCCGGGC
    CGCCCCGCCG CCCAGGCGCG CGTCCTCGGT GGGCCTGCTG CTCCGTGCGG AGGAGCTGAT
    CCTGAAAAAG CCGCGGAGCG AGCTCGTATT CGAGGGGCAG AGGCACCGGC ACGGGGCCTG
    GACTGCAGCT GCCCTCTGCC AGAGCCTGGG CGCCGCCGCC CCCGAGATCC GCTGCTGCGT
    GGAGGCTGTG AACTTCGTGG CCGAGAGCAC ACGGAGCCAG GAGGCCTCCG TCGAGGAGGT
    GTCCGACTGG GTGCGCATGG GGAAGGCCCT GGATCACCTC TGCTTCTGGG CCGCCTTGGT
    GCTGTTCGGC GTGGGGTCCA GCGTCATTTT CCTTGGGGGC TACTTCAACC AAGTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274174.1
    CDS321..1940
    Translation

    Target ORF information:

    RefSeq Version XM_022418466.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418466.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGAACGA GAAGGAGGAG
    ACCCTGACCA CCAGCGTCTG GATTGGAATC GACTGGCAGG ATTACCGACT CAACTACAGC
    AAGGACGACT TCGGGGGCGT GGAGACCTTG CGGGTCCCTT CGGAGCTCGT ATGGCTCCCG
    GAGATTGTGC TGGAGAACAA CATTGACGGC CAGTTCGGCG TGGCCTACGA CGCCAACGTG
    CTGATCTACG AGGGCGGCTA CGTGAGCTGG CTGCCCCCCG CCATCTACCG CAGCACCTGC
    GCCGTGGAGG TCACCTACTT CCCCTTCGAC TGGCAGAACT GCTCGCTGGT CTTCCGCTCG
    CAGACGTACA ATGCCGAGGA GGTGGACTTC GTCTTCGCCG TGGACGACGA CGGCGAGACC
    ATCAGCAAAA TCGACATCGA CACCGAGGCC TACACGGAGA ACGGCGAGTG GGCCATCGAC
    TTCTGCCCCG GGCTGATCCG TCGCCCCAAC GGAGCCTCCG CTGGGGACCC GGGGGTCGTC
    GACATCATCT ACACGCTCAT CATCCGCCGG AAGCCGCTCT TCTACGTCAT CAACATCATC
    GTGCCCTGCG TGCTCATCTC GGGCCTGGTG CTGCTCGCCT ACTTCCTGCC CGCGCAGGGT
    AAGGGGCCGC GGGACCCTGA GCCCGGCTCG CTGCTGGCGG GGCGGCTCTC ACCGGGCGCT
    CCCTCCAGCC GGCGGCCAGA AGTGCACCGT TTCCATCAAC GTCCTACTCG CCCAGACCGT
    CTTCCTGTTC CTGATCGCTC AGAAAATCCC CGAGACGTCG CTGAGCGTGC CGATGCTGGG
    CAGGTACCTC ATCTTCGTCA TGGTGGTTGC CACGATCATC GTCATGAACT GTGTCATCGT
    GCTCAACGTG TCTCTGCGGA CGCCCACCAC ACACGCCATG TCCCCGCGGC TGCGCCACGT
    CCTGCTGGAG CAGCTGCCCA GCCTCCTGGG CTCCAGCGCA CCCCCCGAGG CTCCCCGGGC
    CGCCCCGCCG CCCAGGCGCG CGTCCTCGGT GGGCCTGCTG CTCCGTGCGG AGGAGCTGAT
    CCTGAAAAAG CCGCGGAGCG AGCTCGTATT CGAGGGGCAG AGGCACCGGC ACGGGGCCTG
    GACTGCAGCT GCCCTCTGCC AGAGCCTGGG CGCCGCCGCC CCCGAGATCC GCTGCTGCGT
    GGAGGCTGTG AACTTCGTGG CCGAGAGCAC ACGGAGCCAG GAGGCCTCCG TCGAGGAGGT
    GTCCGACTGG GTGCGCATGG GGAAGGCCCT GGATCACCTC TGCTTCTGGG CCGCCTTGGT
    GCTGTTCGGC GTGGGGTCCA GCGTCATTTT CCTTGGGGGC TACTTCAACC AAGTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288090
    Clone ID Related Accession (Same CDS sequence) XM_022418467.1
    Accession Version XM_022418467.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product acetylcholine receptor subunit epsilon isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCAGGGG CTCTGCTTGG TGCCCTGCTC CTCCTGCAGC TCCTGGCAGG TGGAGCTGAG 
    GCCAAGAACG AGGAGCTGCA TCTGTACCAC CATCTCTTCG ACAACTATGA CCCAGGAAGC
    CGGCCCGTGA AGGAGCCTGA AGACACGGTC ACCATCACCC TCAAGGTCAC CCTGACCAAC
    CTTATCTCCC TGGTAAGATG CCCCCACCCG CACCCACCGT CCTGGGGGCT AGTTCCGACC
    TCCACCACCC TGACTCCGGC TCGTCTCCAG AACGAGAAGG AGGAGACCCT GACCACCAGC
    GTCTGGATTG GAATCGACTG GCAGGATTAC CGACTCAACT ACAGCAAGGA CGACTTCGGG
    GGCGTGGAGA CCTTGCGGGT CCCTTCGGAG CTCGTATGGC TCCCGGAGAT TGTGCTGGAG
    AACAACATTG ACGGCCAGTT CGGCGTGGCC TACGACGCCA ACGTGCTGAT CTACGAGGGC
    GGCTACGTGA GCTGGCTGCC CCCCGCCATC TACCGCAGCA CCTGCGCCGT GGAGGTCACC
    TACTTCCCCT TCGACTGGCA GAACTGCTCG CTGGTCTTCC GCTCGCAGAC GTACAATGCC
    GAGGAGGTGG ACTTCGTCTT CGCCGTGGAC GACGACGGCG AGACCATCAG CAAAATCGAC
    ATCGACACCG AGGCCTACAC GGAGAACGGC GAGTGGGCCA TCGACTTCTG CCCCGGGCTG
    ATCCGTCGCC CCAACGGAGC CTCCGCTGGG GACCCGGGGG TCGTCGACAT CATCTACACG
    CTCATCATCC GCCGGAAGCC GCTCTTCTAC GTCATCAACA TCATCGTGCC CTGCGTGCTC
    ATCTCGGGCC TGGTGCTGCT CGCCTACTTC CTGCCCGCGC AGGGTAAGGG GCCGCGGGAC
    CCTGAGCCCG GCTCGCTGCT GGCGGGGCGG CTCTCACCGG GCGCTCCCTC CAGCCGGCGG
    CCAGAAGTGC ACCGTTTCCA TCAACGTCCT ACTCGCCCAG ACCGTCTTCC TGTTCCTGAT
    CGCTCAGAAA ATCCCCGAGA CGTCGCTGAG CGTGCCGATG CTGGGCAGGT ACCTCATCTT
    CGTCATGGTG GTTGCCACGA TCATCGTCAT GAACTGTGTC ATCGTGCTCA ACGTGTCTCT
    GCGGACGCCC ACCACACACG CCATGTCCCC GCGGCTGCGC CACGTCCTGC TGGAGCAGCT
    GCCCAGCCTC CTGGGCTCCA GCGCACCCCC CGAGGCTCCC CGGGCCGCCC CGCCGCCCAG
    GCGCGCGTCC TCGGTGGGCC TGCTGCTCCG TGCGGAGGAG CTGATCCTGA AAAAGCCGCG
    GAGCGAGCTC GTATTCGAGG GGCAGAGGCA CCGGCACGGG GCCTGGACTG CAGCTGCCCT
    CTGCCAGAGC CTGGGCGCCG CCGCCCCCGA GATCCGCTGC TGCGTGGAGG CTGTGAACTT
    CGTGGCCGAG AGCACACGGA GCCAGGAGGC CTCCGTCGAG GAGGTGTCCG ACTGGGTGCG
    CATGGGGAAG GCCCTGGATC ACCTCTGCTT CTGGGCCGCC TTGGTGCTGT TCGGCGTGGG
    GTCCAGCGTC ATTTTCCTTG GGGGCTACTT CAACCAAGTG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274175.1
    CDS64..1668
    Translation

    Target ORF information:

    RefSeq Version XM_022418467.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418467.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCAGGGG CTCTGCTTGG TGCCCTGCTC CTCCTGCAGC TCCTGGCAGG TGGAGCTGAG 
    GCCAAGAACG AGGAGCTGCA TCTGTACCAC CATCTCTTCG ACAACTATGA CCCAGGAAGC
    CGGCCCGTGA AGGAGCCTGA AGACACGGTC ACCATCACCC TCAAGGTCAC CCTGACCAAC
    CTTATCTCCC TGGTAAGATG CCCCCACCCG CACCCACCGT CCTGGGGGCT AGTTCCGACC
    TCCACCACCC TGACTCCGGC TCGTCTCCAG AACGAGAAGG AGGAGACCCT GACCACCAGC
    GTCTGGATTG GAATCGACTG GCAGGATTAC CGACTCAACT ACAGCAAGGA CGACTTCGGG
    GGCGTGGAGA CCTTGCGGGT CCCTTCGGAG CTCGTATGGC TCCCGGAGAT TGTGCTGGAG
    AACAACATTG ACGGCCAGTT CGGCGTGGCC TACGACGCCA ACGTGCTGAT CTACGAGGGC
    GGCTACGTGA GCTGGCTGCC CCCCGCCATC TACCGCAGCA CCTGCGCCGT GGAGGTCACC
    TACTTCCCCT TCGACTGGCA GAACTGCTCG CTGGTCTTCC GCTCGCAGAC GTACAATGCC
    GAGGAGGTGG ACTTCGTCTT CGCCGTGGAC GACGACGGCG AGACCATCAG CAAAATCGAC
    ATCGACACCG AGGCCTACAC GGAGAACGGC GAGTGGGCCA TCGACTTCTG CCCCGGGCTG
    ATCCGTCGCC CCAACGGAGC CTCCGCTGGG GACCCGGGGG TCGTCGACAT CATCTACACG
    CTCATCATCC GCCGGAAGCC GCTCTTCTAC GTCATCAACA TCATCGTGCC CTGCGTGCTC
    ATCTCGGGCC TGGTGCTGCT CGCCTACTTC CTGCCCGCGC AGGGTAAGGG GCCGCGGGAC
    CCTGAGCCCG GCTCGCTGCT GGCGGGGCGG CTCTCACCGG GCGCTCCCTC CAGCCGGCGG
    CCAGAAGTGC ACCGTTTCCA TCAACGTCCT ACTCGCCCAG ACCGTCTTCC TGTTCCTGAT
    CGCTCAGAAA ATCCCCGAGA CGTCGCTGAG CGTGCCGATG CTGGGCAGGT ACCTCATCTT
    CGTCATGGTG GTTGCCACGA TCATCGTCAT GAACTGTGTC ATCGTGCTCA ACGTGTCTCT
    GCGGACGCCC ACCACACACG CCATGTCCCC GCGGCTGCGC CACGTCCTGC TGGAGCAGCT
    GCCCAGCCTC CTGGGCTCCA GCGCACCCCC CGAGGCTCCC CGGGCCGCCC CGCCGCCCAG
    GCGCGCGTCC TCGGTGGGCC TGCTGCTCCG TGCGGAGGAG CTGATCCTGA AAAAGCCGCG
    GAGCGAGCTC GTATTCGAGG GGCAGAGGCA CCGGCACGGG GCCTGGACTG CAGCTGCCCT
    CTGCCAGAGC CTGGGCGCCG CCGCCCCCGA GATCCGCTGC TGCGTGGAGG CTGTGAACTT
    CGTGGCCGAG AGCACACGGA GCCAGGAGGC CTCCGTCGAG GAGGTGTCCG ACTGGGTGCG
    CATGGGGAAG GCCCTGGATC ACCTCTGCTT CTGGGCCGCC TTGGTGCTGT TCGGCGTGGG
    GTCCAGCGTC ATTTTCCTTG GGGGCTACTT CAACCAAGTG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288091
    Clone ID Related Accession (Same CDS sequence) XM_014113501.2
    Accession Version XM_014113501.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product acetylcholine receptor subunit epsilon isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGAACGA GAAGGAGGAG
    ACCCTGACCA CCAGCGTCTG GATTGGAATC GACTGGCAGG ATTACCGACT CAACTACAGC
    AAGGACGACT TCGGGGGCGT GGAGACCTTG CGGGTCCCTT CGGAGCTCGT ATGGCTCCCG
    GAGATTGTGC TGGAGAACAA CATTGACGGC CAGTTCGGCG TGGCCTACGA CGCCAACGTG
    CTGATCTACG AGGGCGGCTA CGTGAGCTGG CTGCCCCCCG CCATCTACCG CAGCACCTGC
    GCCGTGGAGG TCACCTACTT CCCCTTCGAC TGGCAGAACT GCTCGCTGGT CTTCCGCTCG
    CAGACGTACA ATGCCGAGGA GGTGGACTTC GTCTTCGCCG TGGACGACGA CGGCGAGACC
    ATCAGCAAAA TCGACATCGA CACCGAGGCC TACACGGAGA ACGGCGAGTG GGCCATCGAC
    TTCTGCCCCG GGCTGATCCG TCGCCCCAAC GGAGCCTCCG CTGGGGACCC GGGGGTCGTC
    GACATCATCT ACACGCTCAT CATCCGCCGG AAGCCGCTCT TCTACGTCAT CAACATCATC
    GTGCCCTGCG TGCTCATCTC GGGCCTGGTG CTGCTCGCCT ACTTCCTGCC CGCGCAGGCC
    GGCGGCCAGA AGTGCACCGT TTCCATCAAC GTCCTACTCG CCCAGACCGT CTTCCTGTTC
    CTGATCGCTC AGAAAATCCC CGAGACGTCG CTGAGCGTGC CGATGCTGGG CAGGTACCTC
    ATCTTCGTCA TGGTGGTTGC CACGATCATC GTCATGAACT GTGTCATCGT GCTCAACGTG
    TCTCTGCGGA CGCCCACCAC ACACGCCATG TCCCCGCGGC TGCGCCACGT CCTGCTGGAG
    CAGCTGCCCA GCCTCCTGGG CTCCAGCGCA CCCCCCGAGG CTCCCCGGGC CGCCCCGCCG
    CCCAGGCGCG CGTCCTCGGT GGGCCTGCTG CTCCGTGCGG AGGAGCTGAT CCTGAAAAAG
    CCGCGGAGCG AGCTCGTATT CGAGGGGCAG AGGCACCGGC ACGGGGCCTG GACTGCAGCT
    GCCCTCTGCC AGAGCCTGGG CGCCGCCGCC CCCGAGATCC GCTGCTGCGT GGAGGCTGTG
    AACTTCGTGG CCGAGAGCAC ACGGAGCCAG GAGGCCTCCG TCGAGGAGGT GTCCGACTGG
    GTGCGCATGG GGAAGGCCCT GGATCACCTC TGCTTCTGGG CCGCCTTGGT GCTGTTCGGC
    GTGGGGTCCA GCGTCATTTT CCTTGGGGGC TACTTCAACC AAGTGCCTGA GCTGCCCTAC
    CCACCGTGCA TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968976.2
    CDS319..1893
    Translation

    Target ORF information:

    RefSeq Version XM_014113501.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113501.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGAACGA GAAGGAGGAG
    ACCCTGACCA CCAGCGTCTG GATTGGAATC GACTGGCAGG ATTACCGACT CAACTACAGC
    AAGGACGACT TCGGGGGCGT GGAGACCTTG CGGGTCCCTT CGGAGCTCGT ATGGCTCCCG
    GAGATTGTGC TGGAGAACAA CATTGACGGC CAGTTCGGCG TGGCCTACGA CGCCAACGTG
    CTGATCTACG AGGGCGGCTA CGTGAGCTGG CTGCCCCCCG CCATCTACCG CAGCACCTGC
    GCCGTGGAGG TCACCTACTT CCCCTTCGAC TGGCAGAACT GCTCGCTGGT CTTCCGCTCG
    CAGACGTACA ATGCCGAGGA GGTGGACTTC GTCTTCGCCG TGGACGACGA CGGCGAGACC
    ATCAGCAAAA TCGACATCGA CACCGAGGCC TACACGGAGA ACGGCGAGTG GGCCATCGAC
    TTCTGCCCCG GGCTGATCCG TCGCCCCAAC GGAGCCTCCG CTGGGGACCC GGGGGTCGTC
    GACATCATCT ACACGCTCAT CATCCGCCGG AAGCCGCTCT TCTACGTCAT CAACATCATC
    GTGCCCTGCG TGCTCATCTC GGGCCTGGTG CTGCTCGCCT ACTTCCTGCC CGCGCAGGCC
    GGCGGCCAGA AGTGCACCGT TTCCATCAAC GTCCTACTCG CCCAGACCGT CTTCCTGTTC
    CTGATCGCTC AGAAAATCCC CGAGACGTCG CTGAGCGTGC CGATGCTGGG CAGGTACCTC
    ATCTTCGTCA TGGTGGTTGC CACGATCATC GTCATGAACT GTGTCATCGT GCTCAACGTG
    TCTCTGCGGA CGCCCACCAC ACACGCCATG TCCCCGCGGC TGCGCCACGT CCTGCTGGAG
    CAGCTGCCCA GCCTCCTGGG CTCCAGCGCA CCCCCCGAGG CTCCCCGGGC CGCCCCGCCG
    CCCAGGCGCG CGTCCTCGGT GGGCCTGCTG CTCCGTGCGG AGGAGCTGAT CCTGAAAAAG
    CCGCGGAGCG AGCTCGTATT CGAGGGGCAG AGGCACCGGC ACGGGGCCTG GACTGCAGCT
    GCCCTCTGCC AGAGCCTGGG CGCCGCCGCC CCCGAGATCC GCTGCTGCGT GGAGGCTGTG
    AACTTCGTGG CCGAGAGCAC ACGGAGCCAG GAGGCCTCCG TCGAGGAGGT GTCCGACTGG
    GTGCGCATGG GGAAGGCCCT GGATCACCTC TGCTTCTGGG CCGCCTTGGT GCTGTTCGGC
    GTGGGGTCCA GCGTCATTTT CCTTGGGGGC TACTTCAACC AAGTGCCTGA GCTGCCCTAC
    CCACCGTGCA TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288092
    Clone ID Related Accession (Same CDS sequence) XM_014113502.2
    Accession Version XM_014113502.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1572bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product acetylcholine receptor subunit epsilon isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGAACGA GAAGGAGGAG
    ACCCTGACCA CCAGCGTCTG GATTGGAATC GACTGGCAGG ATTACCGACT CAACTACAGC
    AAGGACGACT TCGGGGGCGT GGAGACCTTG CGGGTCCCTT CGGAGCTCGT ATGGCTCCCG
    GAGATTGTGC TGGAGAACAA CATTGACGGC CAGTTCGGCG TGGCCTACGA CGCCAACGTG
    CTGATCTACG AGGGCGGCTA CGTGAGCTGG CTGCCCCCCG CCATCTACCG CAGCACCTGC
    GCCGTGGAGG TCACCTACTT CCCCTTCGAC TGGCAGAACT GCTCGCTGGT CTTCCGCTCG
    CAGACGTACA ATGCCGAGGA GGTGGACTTC GTCTTCGCCG TGGACGACGA CGGCGAGACC
    ATCAGCAAAA TCGACATCGA CACCGAGGCC TACACGGAGA ACGGCGAGTG GGCCATCGAC
    TTCTGCCCCG GGCTGATCCG TCGCCCCAAC GGAGCCTCCG CTGGGGACCC GGGGGTCGTC
    GACATCATCT ACACGCTCAT CATCCGCCGG AAGCCGCTCT TCTACGTCAT CAACATCATC
    GTGCCCTGCG TGCTCATCTC GGGCCTGGTG CTGCTCGCCT ACTTCCTGCC CGCGCAGGCC
    GGCGGCCAGA AGTGCACCGT TTCCATCAAC GTCCTACTCG CCCAGACCGT CTTCCTGTTC
    CTGATCGCTC AGAAAATCCC CGAGACGTCG CTGAGCGTGC CGATGCTGGG CAGGTACCTC
    ATCTTCGTCA TGGTGGTTGC CACGATCATC GTCATGAACT GTGTCATCGT GCTCAACGTG
    TCTCTGCGGA CGCCCACCAC ACACGCCATG TCCCCGCGGC TGCGCCACGT CCTGCTGGAG
    CAGCTGCCCA GCCTCCTGGG CTCCAGCGCA CCCCCCGAGG CTCCCCGGGC CGCCCCGCCG
    CCCAGGCGCG CGTCCTCGGT GGGCCTGCTG CTCCGTGCGG AGGAGCTGAT CCTGAAAAAG
    CCGCGGAGCG AGCTCGTATT CGAGGGGCAG AGGCACCGGC ACGGGGCCTG GACTGCTGCC
    CTCTGCCAGA GCCTGGGCGC CGCCGCCCCC GAGATCCGCT GCTGCGTGGA GGCTGTGAAC
    TTCGTGGCCG AGAGCACACG GAGCCAGGAG GCCTCCGTCG AGGAGGTGTC CGACTGGGTG
    CGCATGGGGA AGGCCCTGGA TCACCTCTGC TTCTGGGCCG CCTTGGTGCT GTTCGGCGTG
    GGGTCCAGCG TCATTTTCCT TGGGGGCTAC TTCAACCAAG TGCCTGAGCT GCCCTACCCA
    CCGTGCATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968977.2
    CDS316..1887
    Translation

    Target ORF information:

    RefSeq Version XM_014113502.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113502.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGAACGA GAAGGAGGAG
    ACCCTGACCA CCAGCGTCTG GATTGGAATC GACTGGCAGG ATTACCGACT CAACTACAGC
    AAGGACGACT TCGGGGGCGT GGAGACCTTG CGGGTCCCTT CGGAGCTCGT ATGGCTCCCG
    GAGATTGTGC TGGAGAACAA CATTGACGGC CAGTTCGGCG TGGCCTACGA CGCCAACGTG
    CTGATCTACG AGGGCGGCTA CGTGAGCTGG CTGCCCCCCG CCATCTACCG CAGCACCTGC
    GCCGTGGAGG TCACCTACTT CCCCTTCGAC TGGCAGAACT GCTCGCTGGT CTTCCGCTCG
    CAGACGTACA ATGCCGAGGA GGTGGACTTC GTCTTCGCCG TGGACGACGA CGGCGAGACC
    ATCAGCAAAA TCGACATCGA CACCGAGGCC TACACGGAGA ACGGCGAGTG GGCCATCGAC
    TTCTGCCCCG GGCTGATCCG TCGCCCCAAC GGAGCCTCCG CTGGGGACCC GGGGGTCGTC
    GACATCATCT ACACGCTCAT CATCCGCCGG AAGCCGCTCT TCTACGTCAT CAACATCATC
    GTGCCCTGCG TGCTCATCTC GGGCCTGGTG CTGCTCGCCT ACTTCCTGCC CGCGCAGGCC
    GGCGGCCAGA AGTGCACCGT TTCCATCAAC GTCCTACTCG CCCAGACCGT CTTCCTGTTC
    CTGATCGCTC AGAAAATCCC CGAGACGTCG CTGAGCGTGC CGATGCTGGG CAGGTACCTC
    ATCTTCGTCA TGGTGGTTGC CACGATCATC GTCATGAACT GTGTCATCGT GCTCAACGTG
    TCTCTGCGGA CGCCCACCAC ACACGCCATG TCCCCGCGGC TGCGCCACGT CCTGCTGGAG
    CAGCTGCCCA GCCTCCTGGG CTCCAGCGCA CCCCCCGAGG CTCCCCGGGC CGCCCCGCCG
    CCCAGGCGCG CGTCCTCGGT GGGCCTGCTG CTCCGTGCGG AGGAGCTGAT CCTGAAAAAG
    CCGCGGAGCG AGCTCGTATT CGAGGGGCAG AGGCACCGGC ACGGGGCCTG GACTGCTGCC
    CTCTGCCAGA GCCTGGGCGC CGCCGCCCCC GAGATCCGCT GCTGCGTGGA GGCTGTGAAC
    TTCGTGGCCG AGAGCACACG GAGCCAGGAG GCCTCCGTCG AGGAGGTGTC CGACTGGGTG
    CGCATGGGGA AGGCCCTGGA TCACCTCTGC TTCTGGGCCG CCTTGGTGCT GTTCGGCGTG
    GGGTCCAGCG TCATTTTCCT TGGGGGCTAC TTCAACCAAG TGCCTGAGCT GCCCTACCCA
    CCGTGCATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36390
    Clone ID Related Accession (Same CDS sequence) XM_014113500.1
    Accession Version XM_014113500.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product acetylcholine receptor subunit epsilon isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCCCCCAC CCGCACCCAC CGTCCTGGGG GCTAGTTCCG ACCTCCACCA CCCTGACTCC 
    GGCTCGTCTC CAGAACGAGA AGGAGGAGAC CCTGACCACC AGCGTCTGGA TTGGAATCGA
    CTGGCAGGAT TACCGACTCA ACTACAGCAA GGACGACTTC GGGGGCGTGG AGACCTTGCG
    GGTCCCTTCG GAGCTCGTAT GGCTCCCGGA GATTGTGCTG GAGAACAAGT GAGGCCGCGG
    CCCTGCACCT GGCGGGCGGG GCCGGGGTTG GGCCCGGGCA GACGCCGGCT CTCTCGCGGC
    GCCCACAGCA TTGACGGCCA GTTCGGCGTG GCCTACGACG CCAACGTGCT GATCTACGAG
    GGCGGCTACG TGAGCTGGCT GCCCCCCGCC ATCTACCGCA GCACCTGCGC CGTGGAGGTC
    ACCTACTTCC CCTTCGACTG GCAGAACTGC TCGCTGGTCT TCCGGTGGGA CAAGCTGCTC
    AGGGGCGGGG GGCAGGGAAG GGGCTGGGGG GCGGTGGGCA GGAGGGCAGG GGGCAGGGGG
    CGGGGCGGGG CCGCCCGGGA ATGGGATGTG GGGGTCCCGC CTCCGAGCTC CTGGGGGGGG
    GCGCCGGGCC CTGGCTCCGC AGCTCGCAGA CGTACAATGC CGAGGAGGTG GACTTCGTCT
    TCGCCGTGGA CGACGACGGC GAGACCATCA GCAAAATCGA CATCGACACC GAGGCCTACA
    CGGGTGAGGC CCCCTCTGCT CCGAAAACCC GCCCCTGCAC GGGCCCGGGG TGGGGGTGGG
    GTACTGGCCA GCTGGGGCGA CCGCAGACCG TGGAGAGCAG GGTGGACGCG GCCGCCGCCC
    TGCTCGAAAG CACTCCCGAC CGTCCCGGGC CCCGTCCCTC CCACCCCTTC CCGCCCCGCA
    GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCCGGGCTGA TCCGTCGCCC CAACGGAGCC
    TCCGCTGGGG ACCCGGGGGT CGTCGACATC ATCTACACGC TCATCATCCG CCGGAAGCCG
    CTCTTCTACG TCATCAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC
    GCCTACTTCC TGCCCGCGCA GGGTAAGGGG CCGCGGGACC CTGAGCCCGG CTCGCTGCTG
    GCGGGGCGGC TCTCACCGGG CGCTCCCTCC AGCCGGCGGC CAGAAGTGCA CCGTTTCCAT
    CAACGTCCTA CTCGCCCAGA CCGTCTTCCT GTTCCTGATC GCTCAGAAAA TCCCCGAGAC
    GTCGCTGAGC GTGCCGATGC TGGGCAGGTA CCTCATCTTC GTCATGGTGG TTGCCACGAT
    CATCGTCATG AACTGTGTCA TCGTGCTCAA CGTGTCTCTG CGGACGCCCA CCACACACGC
    CATGTCCCCG CGGCTGCGCC ACGTCCTGCT GGAGCAGCTG CCCAGCCTCC TGGGCTCCAG
    CGCACCCCCC GAGGCTCCCC GGGCCGCCCC GCCGCCCAGG CGCGCGTCCT CGGTGGGCCT
    GCTGCTCCGT GCGGAGGAGC TGATCCTGAA AAAGCCGCGG AGCGAGCTCG TATTCGAGGG
    GCAGAGGCAC CGGCACGGGG CCTGGACTGC AGCTGCCCTC TGCCAGAGCC TGGGCGCCGC
    CGCCCCCGAG ATCCGCTGCT GCGTGGAGGC TGTGAACTTC GTGGCCGAGA GCACACGGAG
    CCAGGAGGCC TCCGTCGAGG AGGTGTCCGA CTGGGTGCGC ATGGGGAAGG CCCTGGATCA
    CCTCTGCTTC TGGGCCGCCT TGGTGCTGTT CGGCGTGGGG TCCAGCGTCA TTTTCCTTGG
    GGGCTACTTC AACCAAGTGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968975.1
    CDS81..1904
    Translation

    Target ORF information:

    RefSeq Version XM_014113500.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113500.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCCCCCAC CCGCACCCAC CGTCCTGGGG GCTAGTTCCG ACCTCCACCA CCCTGACTCC 
    GGCTCGTCTC CAGAACGAGA AGGAGGAGAC CCTGACCACC AGCGTCTGGA TTGGAATCGA
    CTGGCAGGAT TACCGACTCA ACTACAGCAA GGACGACTTC GGGGGCGTGG AGACCTTGCG
    GGTCCCTTCG GAGCTCGTAT GGCTCCCGGA GATTGTGCTG GAGAACAAGT GAGGCCGCGG
    CCCTGCACCT GGCGGGCGGG GCCGGGGTTG GGCCCGGGCA GACGCCGGCT CTCTCGCGGC
    GCCCACAGCA TTGACGGCCA GTTCGGCGTG GCCTACGACG CCAACGTGCT GATCTACGAG
    GGCGGCTACG TGAGCTGGCT GCCCCCCGCC ATCTACCGCA GCACCTGCGC CGTGGAGGTC
    ACCTACTTCC CCTTCGACTG GCAGAACTGC TCGCTGGTCT TCCGGTGGGA CAAGCTGCTC
    AGGGGCGGGG GGCAGGGAAG GGGCTGGGGG GCGGTGGGCA GGAGGGCAGG GGGCAGGGGG
    CGGGGCGGGG CCGCCCGGGA ATGGGATGTG GGGGTCCCGC CTCCGAGCTC CTGGGGGGGG
    GCGCCGGGCC CTGGCTCCGC AGCTCGCAGA CGTACAATGC CGAGGAGGTG GACTTCGTCT
    TCGCCGTGGA CGACGACGGC GAGACCATCA GCAAAATCGA CATCGACACC GAGGCCTACA
    CGGGTGAGGC CCCCTCTGCT CCGAAAACCC GCCCCTGCAC GGGCCCGGGG TGGGGGTGGG
    GTACTGGCCA GCTGGGGCGA CCGCAGACCG TGGAGAGCAG GGTGGACGCG GCCGCCGCCC
    TGCTCGAAAG CACTCCCGAC CGTCCCGGGC CCCGTCCCTC CCACCCCTTC CCGCCCCGCA
    GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCCGGGCTGA TCCGTCGCCC CAACGGAGCC
    TCCGCTGGGG ACCCGGGGGT CGTCGACATC ATCTACACGC TCATCATCCG CCGGAAGCCG
    CTCTTCTACG TCATCAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC
    GCCTACTTCC TGCCCGCGCA GGGTAAGGGG CCGCGGGACC CTGAGCCCGG CTCGCTGCTG
    GCGGGGCGGC TCTCACCGGG CGCTCCCTCC AGCCGGCGGC CAGAAGTGCA CCGTTTCCAT
    CAACGTCCTA CTCGCCCAGA CCGTCTTCCT GTTCCTGATC GCTCAGAAAA TCCCCGAGAC
    GTCGCTGAGC GTGCCGATGC TGGGCAGGTA CCTCATCTTC GTCATGGTGG TTGCCACGAT
    CATCGTCATG AACTGTGTCA TCGTGCTCAA CGTGTCTCTG CGGACGCCCA CCACACACGC
    CATGTCCCCG CGGCTGCGCC ACGTCCTGCT GGAGCAGCTG CCCAGCCTCC TGGGCTCCAG
    CGCACCCCCC GAGGCTCCCC GGGCCGCCCC GCCGCCCAGG CGCGCGTCCT CGGTGGGCCT
    GCTGCTCCGT GCGGAGGAGC TGATCCTGAA AAAGCCGCGG AGCGAGCTCG TATTCGAGGG
    GCAGAGGCAC CGGCACGGGG CCTGGACTGC AGCTGCCCTC TGCCAGAGCC TGGGCGCCGC
    CGCCCCCGAG ATCCGCTGCT GCGTGGAGGC TGTGAACTTC GTGGCCGAGA GCACACGGAG
    CCAGGAGGCC TCCGTCGAGG AGGTGTCCGA CTGGGTGCGC ATGGGGAAGG CCCTGGATCA
    CCTCTGCTTC TGGGCCGCCT TGGTGCTGTT CGGCGTGGGG TCCAGCGTCA TTTTCCTTGG
    GGGCTACTTC AACCAAGTGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36391
    Clone ID Related Accession (Same CDS sequence) XM_014113501.1
    Accession Version XM_014113501.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product acetylcholine receptor subunit epsilon isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGATGA AGACAGCCCG AGCTTCCTTG TCAGCACATC ACAGTGATGG GACAGCAGCT 
    GTCACCTCAG GTGGAGCTGA GGCCAAGAAC GAGGAGCTGC ATCTGTACCA CCATCTCTTC
    GACAACTATG ACCCAGGAAG CCGGCCCGTG AAGGAGCCTG AAGACACGGT CACCATCACC
    CTCAAGGTCA CCCTGACCAA CCTTATCTCC CTGAACGAGA AGGAGGAGAC CCTGACCACC
    AGCGTCTGGA TTGGAATCGA CTGGCAGGAT TACCGACTCA ACTACAGCAA GGACGACTTC
    GGGGGCGTGG AGACCTTGCG GGTCCCTTCG GAGCTCGTAT GGCTCCCGGA GATTGTGCTG
    GAGAACAACA TTGACGGCCA GTTCGGCGTG GCCTACGACG CCAACGTGCT GATCTACGAG
    GGCGGCTACG TGAGCTGGCT GCCCCCCGCC ATCTACCGCA GCACCTGCGC CGTGGAGGTC
    ACCTACTTCC CCTTCGACTG GCAGAACTGC TCGCTGGTCT TCCGCTCGCA GACGTACAAT
    GCCGAGGAGG TGGACTTCGT CTTCGCCGTG GACGACGACG GCGAGACCAT CAGCAAAATC
    GACATCGACA CCGAGGCCTA CACGGAGAAC GGCGAGTGGG CCATCGACTT CTGCCCCGGG
    CTGATCCGTC GCCCCAACGG AGCCTCCGCT GGGGACCCGG GGGTCGTCGA CATCATCTAC
    ACGCTCATCA TCCGCCGGAA GCCGCTCTTC TACGTCATCA ACATCATCGT GCCCTGCGTG
    CTCATCTCGG GCCTGGTGCT GCTCGCCTAC TTCCTGCCCG CGCAGGCCGG CGGCCAGAAG
    TGCACCGTTT CCATCAACGT CCTACTCGCC CAGACCGTCT TCCTGTTCCT GATCGCTCAG
    AAAATCCCCG AGACGTCGCT GAGCGTGCCG ATGCTGGGCA GGTACCTCAT CTTCGTCATG
    GTGGTTGCCA CGATCATCGT CATGAACTGT GTCATCGTGC TCAACGTGTC TCTGCGGACG
    CCCACCACAC ACGCCATGTC CCCGCGGCTG CGCCACGTCC TGCTGGAGCA GCTGCCCAGC
    CTCCTGGGCT CCAGCGCACC CCCCGAGGCT CCCCGGGCCG CCCCGCCGCC CAGGCGCGCG
    TCCTCGGTGG GCCTGCTGCT CCGTGCGGAG GAGCTGATCC TGAAAAAGCC GCGGAGCGAG
    CTCGTATTCG AGGGGCAGAG GCACCGGCAC GGGGCCTGGA CTGCAGCTGC CCTCTGCCAG
    AGCCTGGGCG CCGCCGCCCC CGAGATCCGC TGCTGCGTGG AGGCTGTGAA CTTCGTGGCC
    GAGAGCACAC GGAGCCAGGA GGCCTCCGTC GAGGAGGTGT CCGACTGGGT GCGCATGGGG
    AAGGCCCTGG ATCACCTCTG CTTCTGGGCC GCCTTGGTGC TGTTCGGCGT GGGGTCCAGC
    GTCATTTTCC TTGGGGGCTA CTTCAACCAA GTGCCTGAGC TGCCCTACCC ACCGTGCATG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968976.1
    CDS10..1512
    Translation

    Target ORF information:

    RefSeq Version XM_014113501.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113501.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGATGA AGACAGCCCG AGCTTCCTTG TCAGCACATC ACAGTGATGG GACAGCAGCT 
    GTCACCTCAG GTGGAGCTGA GGCCAAGAAC GAGGAGCTGC ATCTGTACCA CCATCTCTTC
    GACAACTATG ACCCAGGAAG CCGGCCCGTG AAGGAGCCTG AAGACACGGT CACCATCACC
    CTCAAGGTCA CCCTGACCAA CCTTATCTCC CTGAACGAGA AGGAGGAGAC CCTGACCACC
    AGCGTCTGGA TTGGAATCGA CTGGCAGGAT TACCGACTCA ACTACAGCAA GGACGACTTC
    GGGGGCGTGG AGACCTTGCG GGTCCCTTCG GAGCTCGTAT GGCTCCCGGA GATTGTGCTG
    GAGAACAACA TTGACGGCCA GTTCGGCGTG GCCTACGACG CCAACGTGCT GATCTACGAG
    GGCGGCTACG TGAGCTGGCT GCCCCCCGCC ATCTACCGCA GCACCTGCGC CGTGGAGGTC
    ACCTACTTCC CCTTCGACTG GCAGAACTGC TCGCTGGTCT TCCGCTCGCA GACGTACAAT
    GCCGAGGAGG TGGACTTCGT CTTCGCCGTG GACGACGACG GCGAGACCAT CAGCAAAATC
    GACATCGACA CCGAGGCCTA CACGGAGAAC GGCGAGTGGG CCATCGACTT CTGCCCCGGG
    CTGATCCGTC GCCCCAACGG AGCCTCCGCT GGGGACCCGG GGGTCGTCGA CATCATCTAC
    ACGCTCATCA TCCGCCGGAA GCCGCTCTTC TACGTCATCA ACATCATCGT GCCCTGCGTG
    CTCATCTCGG GCCTGGTGCT GCTCGCCTAC TTCCTGCCCG CGCAGGCCGG CGGCCAGAAG
    TGCACCGTTT CCATCAACGT CCTACTCGCC CAGACCGTCT TCCTGTTCCT GATCGCTCAG
    AAAATCCCCG AGACGTCGCT GAGCGTGCCG ATGCTGGGCA GGTACCTCAT CTTCGTCATG
    GTGGTTGCCA CGATCATCGT CATGAACTGT GTCATCGTGC TCAACGTGTC TCTGCGGACG
    CCCACCACAC ACGCCATGTC CCCGCGGCTG CGCCACGTCC TGCTGGAGCA GCTGCCCAGC
    CTCCTGGGCT CCAGCGCACC CCCCGAGGCT CCCCGGGCCG CCCCGCCGCC CAGGCGCGCG
    TCCTCGGTGG GCCTGCTGCT CCGTGCGGAG GAGCTGATCC TGAAAAAGCC GCGGAGCGAG
    CTCGTATTCG AGGGGCAGAG GCACCGGCAC GGGGCCTGGA CTGCAGCTGC CCTCTGCCAG
    AGCCTGGGCG CCGCCGCCCC CGAGATCCGC TGCTGCGTGG AGGCTGTGAA CTTCGTGGCC
    GAGAGCACAC GGAGCCAGGA GGCCTCCGTC GAGGAGGTGT CCGACTGGGT GCGCATGGGG
    AAGGCCCTGG ATCACCTCTG CTTCTGGGCC GCCTTGGTGC TGTTCGGCGT GGGGTCCAGC
    GTCATTTTCC TTGGGGGCTA CTTCAACCAA GTGCCTGAGC TGCCCTACCC ACCGTGCATG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36392
    Clone ID Related Accession (Same CDS sequence) XM_014113502.1
    Accession Version XM_014113502.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product acetylcholine receptor subunit epsilon isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGGATGA AGACAGCCCG AGCTTCCTTG TCAGCACATC ACAGTGATGG GACAGCAGCT 
    GTCACCTCAG GTGGAGCTGA GGCCAAGAAC GAGGAGCTGC ATCTGTACCA CCATCTCTTC
    GACAACTATG ACCCAGGAAG CCGGCCCGTG AAGGAGCCTG AAGACACGGT CACCATCACC
    CTCAAGGTCA CCCTGACCAA CCTTATCTCC CTGAACGAGA AGGAGGAGAC CCTGACCACC
    AGCGTCTGGA TTGGAATCGA CTGGCAGGAT TACCGACTCA ACTACAGCAA GGACGACTTC
    GGGGGCGTGG AGACCTTGCG GGTCCCTTCG GAGCTCGTAT GGCTCCCGGA GATTGTGCTG
    GAGAACAACA TTGACGGCCA GTTCGGCGTG GCCTACGACG CCAACGTGCT GATCTACGAG
    GGCGGCTACG TGAGCTGGCT GCCCCCCGCC ATCTACCGCA GCACCTGCGC CGTGGAGGTC
    ACCTACTTCC CCTTCGACTG GCAGAACTGC TCGCTGGTCT TCCGCTCGCA GACGTACAAT
    GCCGAGGAGG TGGACTTCGT CTTCGCCGTG GACGACGACG GCGAGACCAT CAGCAAAATC
    GACATCGACA CCGAGGCCTA CACGGAGAAC GGCGAGTGGG CCATCGACTT CTGCCCCGGG
    CTGATCCGTC GCCCCAACGG AGCCTCCGCT GGGGACCCGG GGGTCGTCGA CATCATCTAC
    ACGCTCATCA TCCGCCGGAA GCCGCTCTTC TACGTCATCA ACATCATCGT GCCCTGCGTG
    CTCATCTCGG GCCTGGTGCT GCTCGCCTAC TTCCTGCCCG CGCAGGCCGG CGGCCAGAAG
    TGCACCGTTT CCATCAACGT CCTACTCGCC CAGACCGTCT TCCTGTTCCT GATCGCTCAG
    AAAATCCCCG AGACGTCGCT GAGCGTGCCG ATGCTGGGCA GGTACCTCAT CTTCGTCATG
    GTGGTTGCCA CGATCATCGT CATGAACTGT GTCATCGTGC TCAACGTGTC TCTGCGGACG
    CCCACCACAC ACGCCATGTC CCCGCGGCTG CGCCACGTCC TGCTGGAGCA GCTGCCCAGC
    CTCCTGGGCT CCAGCGCACC CCCCGAGGCT CCCCGGGCCG CCCCGCCGCC CAGGCGCGCG
    TCCTCGGTGG GCCTGCTGCT CCGTGCGGAG GAGCTGATCC TGAAAAAGCC GCGGAGCGAG
    CTCGTATTCG AGGGGCAGAG GCACCGGCAC GGGGCCTGGA CTGCTGCCCT CTGCCAGAGC
    CTGGGCGCCG CCGCCCCCGA GATCCGCTGC TGCGTGGAGG CTGTGAACTT CGTGGCCGAG
    AGCACACGGA GCCAGGAGGC CTCCGTCGAG GAGGTGTCCG ACTGGGTGCG CATGGGGAAG
    GCCCTGGATC ACCTCTGCTT CTGGGCCGCC TTGGTGCTGT TCGGCGTGGG GTCCAGCGTC
    ATTTTCCTTG GGGGCTACTT CAACCAAGTG CCTGAGCTGC CCTACCCACC GTGCATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968977.1
    CDS10..1509
    Translation

    Target ORF information:

    RefSeq Version XM_014113502.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113502.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGGATGA AGACAGCCCG AGCTTCCTTG TCAGCACATC ACAGTGATGG GACAGCAGCT 
    GTCACCTCAG GTGGAGCTGA GGCCAAGAAC GAGGAGCTGC ATCTGTACCA CCATCTCTTC
    GACAACTATG ACCCAGGAAG CCGGCCCGTG AAGGAGCCTG AAGACACGGT CACCATCACC
    CTCAAGGTCA CCCTGACCAA CCTTATCTCC CTGAACGAGA AGGAGGAGAC CCTGACCACC
    AGCGTCTGGA TTGGAATCGA CTGGCAGGAT TACCGACTCA ACTACAGCAA GGACGACTTC
    GGGGGCGTGG AGACCTTGCG GGTCCCTTCG GAGCTCGTAT GGCTCCCGGA GATTGTGCTG
    GAGAACAACA TTGACGGCCA GTTCGGCGTG GCCTACGACG CCAACGTGCT GATCTACGAG
    GGCGGCTACG TGAGCTGGCT GCCCCCCGCC ATCTACCGCA GCACCTGCGC CGTGGAGGTC
    ACCTACTTCC CCTTCGACTG GCAGAACTGC TCGCTGGTCT TCCGCTCGCA GACGTACAAT
    GCCGAGGAGG TGGACTTCGT CTTCGCCGTG GACGACGACG GCGAGACCAT CAGCAAAATC
    GACATCGACA CCGAGGCCTA CACGGAGAAC GGCGAGTGGG CCATCGACTT CTGCCCCGGG
    CTGATCCGTC GCCCCAACGG AGCCTCCGCT GGGGACCCGG GGGTCGTCGA CATCATCTAC
    ACGCTCATCA TCCGCCGGAA GCCGCTCTTC TACGTCATCA ACATCATCGT GCCCTGCGTG
    CTCATCTCGG GCCTGGTGCT GCTCGCCTAC TTCCTGCCCG CGCAGGCCGG CGGCCAGAAG
    TGCACCGTTT CCATCAACGT CCTACTCGCC CAGACCGTCT TCCTGTTCCT GATCGCTCAG
    AAAATCCCCG AGACGTCGCT GAGCGTGCCG ATGCTGGGCA GGTACCTCAT CTTCGTCATG
    GTGGTTGCCA CGATCATCGT CATGAACTGT GTCATCGTGC TCAACGTGTC TCTGCGGACG
    CCCACCACAC ACGCCATGTC CCCGCGGCTG CGCCACGTCC TGCTGGAGCA GCTGCCCAGC
    CTCCTGGGCT CCAGCGCACC CCCCGAGGCT CCCCGGGCCG CCCCGCCGCC CAGGCGCGCG
    TCCTCGGTGG GCCTGCTGCT CCGTGCGGAG GAGCTGATCC TGAAAAAGCC GCGGAGCGAG
    CTCGTATTCG AGGGGCAGAG GCACCGGCAC GGGGCCTGGA CTGCTGCCCT CTGCCAGAGC
    CTGGGCGCCG CCGCCCCCGA GATCCGCTGC TGCGTGGAGG CTGTGAACTT CGTGGCCGAG
    AGCACACGGA GCCAGGAGGC CTCCGTCGAG GAGGTGTCCG ACTGGGTGCG CATGGGGAAG
    GCCCTGGATC ACCTCTGCTT CTGGGCCGCC TTGGTGCTGT TCGGCGTGGG GTCCAGCGTC
    ATTTTCCTTG GGGGCTACTT CAACCAAGTG CCTGAGCTGC CCTACCCACC GTGCATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36393
    Clone ID Related Accession (Same CDS sequence) XM_014113503.1
    Accession Version XM_014113503.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product acetylcholine receptor subunit epsilon isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGGTAAG ATGCCCCCAC
    CCGCACCCAC CGTCCTGGGG GCTAGTTCCG ACCTCCACCA CCCTGACTCC GGCTCGTCTC
    CAGAACGAGA AGGAGGAGAC CCTGACCACC AGCGTCTGGA TTGGAATCGA CTGGCAGGAT
    TACCGACTCA ACTACAGCAA GGACGACTTC GGGGGCGTGG AGACCTTGCG GGTCCCTTCG
    GAGCTCGTAT GGCTCCCGGA GATTGTGCTG GAGAACAACA TTGACGGCCA GTTCGGCGTG
    GCCTACGACG CCAACGTGCT GATCTACGAG GGCGGCTACG TGAGCTGGCT GCCCCCCGCC
    ATCTACCGCA GCACCTGCGC CGTGGAGGTC ACCTACTTCC CCTTCGACTG GCAGAACTGC
    TCGCTGGTCT TCCGGTGGGA CAAGCTGCTC AGGGGCGGGG GGCAGGGAAG GGGCTGGGGG
    GCGGTGGGCA GGAGGGCAGG GGGCAGGGGG CGGGGCGGGG CCGCCCGGGA ATGGGATGTG
    GGGGTCCCGC CTCCGAGCTC CTGGGGGGGG GCGCCGGGCC CTGGCTCCGC AGCTCGCAGA
    CGTACAATGC CGAGGAGGTG GACTTCGTCT TCGCCGTGGA CGACGACGGC GAGACCATCA
    GCAAAATCGA CATCGACACC GAGGCCTACA CGGGTGAGGC CCCCTCTGCT CCGAAAACCC
    GCCCCTGCAC GGGCCCGGGG TGGGGGTGGG GTACTGGCCA GCTGGGGCGA CCGCAGACCG
    TGGAGAGCAG GGTGGACGCG GCCGCCGCCC TGCTCGAAAG CACTCCCGAC CGTCCCGGGC
    CCCGTCCCTC CCACCCCTTC CCGCCCCGCA GAGAACGGCG AGTGGGCCAT CGACTTCTGC
    CCCGGGCTGA TCCGTCGCCC CAACGGAGCC TCCGCTGGGG ACCCGGGGGT CGTCGACATC
    ATCTACACGC TCATCATCCG CCGGAAGCCG CTCTTCTACG TCATCAACAT CATCGTGCCC
    TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC GCCTACTTCC TGCCCGCGCA GGCCGGCGGC
    CAGAAGTGCA CCGTTTCCAT CAACGTCCTA CTCGCCCAGA CCGTCTTCCT GTTCCTGATC
    GCTCAGAAAA TCCCCGAGAC GTCGCTGAGC GTGCCGATGC TGGGCAGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968978.1
    CDS342..1772
    Translation

    Target ORF information:

    RefSeq Version XM_014113503.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113503.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGGAAGT CCCAAGGACT TCAGTTTCCT TTTGCAGGCA GGTTATTTTT GCTGTATGAA 
    GGCTTCTCTC CTATGGGGAT GAAGACAGCC CGAGCTTCCT TGTCAGCACA TCACAGTGAT
    GGGACAGCAG CTGTCACCTC AGGTGGAGCT GAGGCCAAGA ACGAGGAGCT GCATCTGTAC
    CACCATCTCT TCGACAACTA TGACCCAGGA AGCCGGCCCG TGAAGGAGCC TGAAGACACG
    GTCACCATCA CCCTCAAGGT CACCCTGACC AACCTTATCT CCCTGGTAAG ATGCCCCCAC
    CCGCACCCAC CGTCCTGGGG GCTAGTTCCG ACCTCCACCA CCCTGACTCC GGCTCGTCTC
    CAGAACGAGA AGGAGGAGAC CCTGACCACC AGCGTCTGGA TTGGAATCGA CTGGCAGGAT
    TACCGACTCA ACTACAGCAA GGACGACTTC GGGGGCGTGG AGACCTTGCG GGTCCCTTCG
    GAGCTCGTAT GGCTCCCGGA GATTGTGCTG GAGAACAACA TTGACGGCCA GTTCGGCGTG
    GCCTACGACG CCAACGTGCT GATCTACGAG GGCGGCTACG TGAGCTGGCT GCCCCCCGCC
    ATCTACCGCA GCACCTGCGC CGTGGAGGTC ACCTACTTCC CCTTCGACTG GCAGAACTGC
    TCGCTGGTCT TCCGGTGGGA CAAGCTGCTC AGGGGCGGGG GGCAGGGAAG GGGCTGGGGG
    GCGGTGGGCA GGAGGGCAGG GGGCAGGGGG CGGGGCGGGG CCGCCCGGGA ATGGGATGTG
    GGGGTCCCGC CTCCGAGCTC CTGGGGGGGG GCGCCGGGCC CTGGCTCCGC AGCTCGCAGA
    CGTACAATGC CGAGGAGGTG GACTTCGTCT TCGCCGTGGA CGACGACGGC GAGACCATCA
    GCAAAATCGA CATCGACACC GAGGCCTACA CGGGTGAGGC CCCCTCTGCT CCGAAAACCC
    GCCCCTGCAC GGGCCCGGGG TGGGGGTGGG GTACTGGCCA GCTGGGGCGA CCGCAGACCG
    TGGAGAGCAG GGTGGACGCG GCCGCCGCCC TGCTCGAAAG CACTCCCGAC CGTCCCGGGC
    CCCGTCCCTC CCACCCCTTC CCGCCCCGCA GAGAACGGCG AGTGGGCCAT CGACTTCTGC
    CCCGGGCTGA TCCGTCGCCC CAACGGAGCC TCCGCTGGGG ACCCGGGGGT CGTCGACATC
    ATCTACACGC TCATCATCCG CCGGAAGCCG CTCTTCTACG TCATCAACAT CATCGTGCCC
    TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC GCCTACTTCC TGCCCGCGCA GGCCGGCGGC
    CAGAAGTGCA CCGTTTCCAT CAACGTCCTA CTCGCCCAGA CCGTCTTCCT GTTCCTGATC
    GCTCAGAAAA TCCCCGAGAC GTCGCTGAGC GTGCCGATGC TGGGCAGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.