IARS2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	IARS2
Entrez Gene ID	478963
Full Name	isoleucyl-tRNA synthetase 2, mitochondrial
General protein information	Preferred Names isoleucyl-tRNA synthetase 2, mitochondrial Names isoleucyl-tRNA synthetase, mitochondrial isoleucine--tRNA ligase, mitochondrial
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_536121.3	XP_536121.3	isoleucine--tRNA ligase, mitochondrial
XM_536121.4	XP_536121.4	isoleucine--tRNA ligase, mitochondrial

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG5414	NP_648837.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003156	XP_312846.5
Eremothecium gossypii	AGOS_AGL247C	NP_986420.1
Neurospora crassa	NCU00920	XP_964746.2
Arabidopsis thaliana (thale cress)	OVA2	NP_199714.2
Canis lupus familiaris (dog)	IARS2	XP_536121.3
Gallus gallus (chicken)	IARS2	NP_001006397.1
Saccharomyces cerevisiae (baker's yeast)	ISM1	NP_015285.1
Schizosaccharomyces pombe (fission yeast)	SPCC18B5.08c	NP_587938.1
Homo sapiens (human)	IARS2	NP_060530.3
Pan troglodytes (chimpanzee)	IARS2	XP_514209.2
	IARS2	XP_001099877.1
Danio rerio (zebrafish)	iars2	NP_001157270.1
Caenorhabditis elegans (roundworm)	iars-2	NP_001251317.1
Kluyveromyces lactis	KLLA0D11858g	XP_453590.1
Xenopus tropicalis (tropical clawed frog)	iars2	NP_001120515.1
Bos taurus (cattle)	IARS2	NP_001193724.1
Mus musculus (house mouse)	Iars2	NP_941055.1
Rattus norvegicus (Norway rat)	Iars2	XP_006250494.1
Magnaporthe oryzae (rice blast fungus)	MGG_01165	XP_003714047.1
Oryza sativa (rice)	Os02g0778200	NP_001048293.1

Gene Ontology

The following IARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe284955	XM_536121.4 Latest version!	Canis lupus familiaris isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IARS2 ( XM_536121.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_536121.4 Canis lupus familiaris isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.

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CloneID	OCe284955
Clone ID Related Accession (Same CDS sequence)	XM_536121.4
Accession Version	XM_536121.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	isoleucine--tRNA ligase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_536121.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGC TGGGCCGCCA GCAGCCCGAC ACGGAGCTGG AGATCCAGCA GAAATGTGGA 
TTTTCAGAAC TTTATTCGTG GCAAAGAGAA AGAAAAGTAA AAACAGAATT TTGTCTTCAT 
GATGGACCTC CTTATGCAAA CGGTGACCCC CACGTTGGAC ATGCTTTAAA TAAGATTTTG 
AAGGATATAG CCAATCGGTA CCATATGATG AGAGGTTCCA AAGTACATTT TGTGCCAGGC 
TGGGATTGTC ATGGATTACC CATTGAAATA AAAGTATTAT CAGAACTTGG TGGAAAAGCT 
CAGAATCTGT CAGCTGTGGA AATTAGAAAG AAAGCTAGAT CATTTGCTGA AGCAGCAATT 
AAGAAACAGA GATCAGCTTT TATTCGTTGG GGAGTAATGG CAGATTGGAA TAATTGCTAC 
TATACATTTG ATGGAAAATA TGAGGCCAAA CAGTTGAGAA TTTTCTACCA AATGTATGAG 
AAGGGCTTGG TTTATCGATC TTACAAACCG GTGTTTTGGT CTCCCTCATC AAGGACTGCA 
TTGGCTGAAG CAGAACTTGA GTATAATCCT GAACATGTCA GTCGCTCAAT ATATGTAAAA 
TTCCCTCTCC TGAAACCTTC TCCCAAGTTG GTATCTCTCA TAGATGGGTC ATCTCCTGTT 
AGTTTTTTAG TTTGGACCAC CCAGGCTTGG ACAATTCCAG CCAATCAAGC TGTTTGTTAT 
ATGCCAGACT CAAAATATGC TATTGTGAAA TGTTCCACAT CTGGAGATCT GTACGTACTT 
GCTGCAGATA AAGTAACATC CGTTGCTTCT GCTTTGGAAA CAACATTTGA GGTTATCTCA 
ACATTTTCAG GTGCAGATTT GGAAGGTGGT ACTTGTGCTC ATCCTTTAAT TCCTGATAAA 
GCCTCCCCTC TTTTACCTGC AAGTCATGTG ACCATGACAA AGGGAACAGG ATTGGTGCAC 
ACAGCCCCAG CTCATGGTAT GGAGGATTAC AGCGTAGCTT CTCAGCACAA CCTGCCCACG 
GACTGTTTAG TGGATGAAGA TGGAGTTTTC ACAGATGTTG CAGGTCCTAA ACTTCAAAAC 
AAGGCTGTCC TTGAAGAGGG AACAGATGTG GTTATAAAGA TGCTTCAGGC TGCAAAGCAT 
TTGTTGAAAG AGGAGAGATT GGTACACAGC TATCCCTATG ACTGGAGGAC TAAGAAACCA 
GTGGTTATTC GTGCCAGCAA GCAGTGGTTT GTAAATATCA CGGATATCAA GGCTACAGCC 
AAGGAATTGT TAAAAAAAGT GAAATTTATT CCTGGATCAG CACTGAATGC CATGGTCGAA 
ACGATGGACA GACGGCCATA TTGGTGTATA TCAAGGCAAA GAGTTTGGGG TGTTCCAATC 
CCTGTATTTC ATCACAAGAC AAAAGATGAA TTCTTGATCA ACAGCCAGAC CACTGAGCAT 
ATTATTAAAC TAGTGGAACA ACACGGCAGT GATGTCTGGT GGACTCTTCC TCCTGAGCAA 
CTTCTTCCTA AAGAAGTCTT ATCTCAGGTT GGTGGTGCTG ATGCCTTGGA ATATGTGCCT 
GGTCAGGATA TTTTGGACAT CTGGTTTGAT AGTGGAACTT CATGGTCTTA CGTTCTTCCA 
GGTACCGACC AAAGAGCAGA TTTGTACTTG GAAGGAAAAG ACCAACTTGG TGGTTGGTTT 
CAGTCTTCTT TACTAACAAG TGTGGCAACA AGGAAAAAAG CACCTTTTAA GACAGTGGTC 
GTTCATGGAT TTACCCTTGG AGAAAAGGGA GAAAAGATGT CCAAGTCTCT TGGGAATGTC 
ATCGATCCTG ATGTGGTCAT CAATGGAGGA CAAGATCAGA GCGCAGAGCC TCCCTACGGT 
GCTGATGTCC TGCGCTGGTG GGTGGCCGAG TCCAACGTCT TCACTGAAGT GACCATCAGC 
CCGTCTGTAC TTAATGCTGC CAGAGATGAT ATTAGCAAGC TCAGGAATAC ACTCCGCTTT 
CTCTTGGGAA ATGTCGCTGG TTTCAACCCA GAAACAGATT CCATTCCTGT TAACAATATG 
CATGTCATAG ACCAGTACAT GCTGCACTTG CTGCAGGATT TAGCAAACAA GATTACCGAT 
TCATATAAAC AGTATGATTT TGGAAAAGTT GTTCGGCTGT TACGAGCATT TTATACCAGA 
GAGCTCTCTA ACTTTTATTT CAGCATAATC AAAGACAGGC TCTACTGTGA GAAGGCAGGC 
GACCCCAAAC GGCGCTCCTG CCAGACCGCA TTGGCCGAAA TTCTGGATGT CATGGTTCGT 
TCTTTTGCTC CCATTCTTCC TCACTTGGCT GAAGAAGTGT TCCAGCACGT ACCTTATGCT 
AAAGAACCCA AGAGTGTATT TCGTACTGGG TGGATTAGAA CTAGTTCTAT CTGGAAGAAG 
CCTGGATTAG AGGAGGCGAT AGAGAGCACG TGTGCCATGC GAGATTCGTT CCTTGGAAGC 
ATCCCTGGTA AAAACGCAGC CGAGTACGAG GTTATCATTG TGATCGAGCC CGGGCTGCTT 
TTTGAGATAA TAGAGATGCT GCAAGCGGAG GAGACTTCCA GCACCTCTCA GCTGAACGAG 
CTAATGATGG CTTCCCAGAC AACTCTACTC TCTCAGGATC CACCGCTCGT AACCACGGAT 
GTAATCGAGC GCCAAGGGAC GTTCCTGATC AACCTAGAAG GTGGTGATAT TCGCGAAGAG 
TCTTCATATA AAGTAATTGT CATGCCAACT ACCAAAGAAA AATGCCCTCG TTGTTGGAAG 
TACACAGCTG AGTCTTCAGA TACACTCTGC CCCCGGTGCG CAGAAGTCGT CAACGGAAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_536121.4
CDS	66..2888
Translation	MKLLGRQQPDTELEIQQKCGFSELYSWQRERKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRYHMMRGSKVHFVPGWDCHGLPIEIKVLSELGGKAQNLSAVEIRKKARSFAEAAIKKQRSAFIRWGVMADWNNCYYTFDGKYEAKQLRIFYQMYEKGLVYRSYKPVFWSPSSRTALAEAELEYNPEHVSRSIYVKFPLLKPSPKLVSLIDGSSPVSFLVWTTQAWTIPANQAVCYMPDSKYAIVKCSTSGDLYVLAADKVTSVASALETTFEVISTFSGADLEGGTCAHPLIPDKASPLLPASHVTMTKGTGLVHTAPAHGMEDYSVASQHNLPTDCLVDEDGVFTDVAGPKLQNKAVLEEGTDVVIKMLQAAKHLLKEERLVHSYPYDWRTKKPVVIRASKQWFVNITDIKATAKELLKKVKFIPGSALNAMVETMDRRPYWCISRQRVWGVPIPVFHHKTKDEFLINSQTTEHIIKLVEQHGSDVWWTLPPEQLLPKEVLSQVGGADALEYVPGQDILDIWFDSGTSWSYVLPGTDQRADLYLEGKDQLGGWFQSSLLTSVATRKKAPFKTVVVHGFTLGEKGEKMSKSLGNVIDPDVVINGGQDQSAEPPYGADVLRWWVAESNVFTEVTISPSVLNAARDDISKLRNTLRFLLGNVAGFNPETDSIPVNNMHVIDQYMLHLLQDLANKITDSYKQYDFGKVVRLLRAFYTRELSNFYFSIIKDRLYCEKAGDPKRRSCQTALAEILDVMVRSFAPILPHLAEEVFQHVPYAKEPKSVFRTGWIRTSSIWKKPGLEEAIESTCAMRDSFLGSIPGKNAAEYEVIIVIEPGLLFEIIEMLQAEETSSTSQLNELMMASQTTLLSQDPPLVTTDVIERQGTFLINLEGGDIREESSYKVIVMPTTKEKCPRCWKYTAESSDTLCPRCAEVVNGK

Target ORF information:

RefSeq Version	XM_536121.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_536121.4

ORF Insert Sequence:

ATGAAGCTGC TGGGCCGCCA GCAGCCCGAC ACGGAGCTGG AGATCCAGCA GAAATGTGGA 
TTTTCAGAAC TTTATTCGTG GCAAAGAGAA AGAAAAGTAA AAACAGAATT TTGTCTTCAT 
GATGGACCTC CTTATGCAAA CGGTGACCCC CACGTTGGAC ATGCTTTAAA TAAGATTTTG 
AAGGATATAG CCAATCGGTA CCATATGATG AGAGGTTCCA AAGTACATTT TGTGCCAGGC 
TGGGATTGTC ATGGATTACC CATTGAAATA AAAGTATTAT CAGAACTTGG TGGAAAAGCT 
CAGAATCTGT CAGCTGTGGA AATTAGAAAG AAAGCTAGAT CATTTGCTGA AGCAGCAATT 
AAGAAACAGA GATCAGCTTT TATTCGTTGG GGAGTAATGG CAGATTGGAA TAATTGCTAC 
TATACATTTG ATGGAAAATA TGAGGCCAAA CAGTTGAGAA TTTTCTACCA AATGTATGAG 
AAGGGCTTGG TTTATCGATC TTACAAACCG GTGTTTTGGT CTCCCTCATC AAGGACTGCA 
TTGGCTGAAG CAGAACTTGA GTATAATCCT GAACATGTCA GTCGCTCAAT ATATGTAAAA 
TTCCCTCTCC TGAAACCTTC TCCCAAGTTG GTATCTCTCA TAGATGGGTC ATCTCCTGTT 
AGTTTTTTAG TTTGGACCAC CCAGGCTTGG ACAATTCCAG CCAATCAAGC TGTTTGTTAT 
ATGCCAGACT CAAAATATGC TATTGTGAAA TGTTCCACAT CTGGAGATCT GTACGTACTT 
GCTGCAGATA AAGTAACATC CGTTGCTTCT GCTTTGGAAA CAACATTTGA GGTTATCTCA 
ACATTTTCAG GTGCAGATTT GGAAGGTGGT ACTTGTGCTC ATCCTTTAAT TCCTGATAAA 
GCCTCCCCTC TTTTACCTGC AAGTCATGTG ACCATGACAA AGGGAACAGG ATTGGTGCAC 
ACAGCCCCAG CTCATGGTAT GGAGGATTAC AGCGTAGCTT CTCAGCACAA CCTGCCCACG 
GACTGTTTAG TGGATGAAGA TGGAGTTTTC ACAGATGTTG CAGGTCCTAA ACTTCAAAAC 
AAGGCTGTCC TTGAAGAGGG AACAGATGTG GTTATAAAGA TGCTTCAGGC TGCAAAGCAT 
TTGTTGAAAG AGGAGAGATT GGTACACAGC TATCCCTATG ACTGGAGGAC TAAGAAACCA 
GTGGTTATTC GTGCCAGCAA GCAGTGGTTT GTAAATATCA CGGATATCAA GGCTACAGCC 
AAGGAATTGT TAAAAAAAGT GAAATTTATT CCTGGATCAG CACTGAATGC CATGGTCGAA 
ACGATGGACA GACGGCCATA TTGGTGTATA TCAAGGCAAA GAGTTTGGGG TGTTCCAATC 
CCTGTATTTC ATCACAAGAC AAAAGATGAA TTCTTGATCA ACAGCCAGAC CACTGAGCAT 
ATTATTAAAC TAGTGGAACA ACACGGCAGT GATGTCTGGT GGACTCTTCC TCCTGAGCAA 
CTTCTTCCTA AAGAAGTCTT ATCTCAGGTT GGTGGTGCTG ATGCCTTGGA ATATGTGCCT 
GGTCAGGATA TTTTGGACAT CTGGTTTGAT AGTGGAACTT CATGGTCTTA CGTTCTTCCA 
GGTACCGACC AAAGAGCAGA TTTGTACTTG GAAGGAAAAG ACCAACTTGG TGGTTGGTTT 
CAGTCTTCTT TACTAACAAG TGTGGCAACA AGGAAAAAAG CACCTTTTAA GACAGTGGTC 
GTTCATGGAT TTACCCTTGG AGAAAAGGGA GAAAAGATGT CCAAGTCTCT TGGGAATGTC 
ATCGATCCTG ATGTGGTCAT CAATGGAGGA CAAGATCAGA GCGCAGAGCC TCCCTACGGT 
GCTGATGTCC TGCGCTGGTG GGTGGCCGAG TCCAACGTCT TCACTGAAGT GACCATCAGC 
CCGTCTGTAC TTAATGCTGC CAGAGATGAT ATTAGCAAGC TCAGGAATAC ACTCCGCTTT 
CTCTTGGGAA ATGTCGCTGG TTTCAACCCA GAAACAGATT CCATTCCTGT TAACAATATG 
CATGTCATAG ACCAGTACAT GCTGCACTTG CTGCAGGATT TAGCAAACAA GATTACCGAT 
TCATATAAAC AGTATGATTT TGGAAAAGTT GTTCGGCTGT TACGAGCATT TTATACCAGA 
GAGCTCTCTA ACTTTTATTT CAGCATAATC AAAGACAGGC TCTACTGTGA GAAGGCAGGC 
GACCCCAAAC GGCGCTCCTG CCAGACCGCA TTGGCCGAAA TTCTGGATGT CATGGTTCGT 
TCTTTTGCTC CCATTCTTCC TCACTTGGCT GAAGAAGTGT TCCAGCACGT ACCTTATGCT 
AAAGAACCCA AGAGTGTATT TCGTACTGGG TGGATTAGAA CTAGTTCTAT CTGGAAGAAG 
CCTGGATTAG AGGAGGCGAT AGAGAGCACG TGTGCCATGC GAGATTCGTT CCTTGGAAGC 
ATCCCTGGTA AAAACGCAGC CGAGTACGAG GTTATCATTG TGATCGAGCC CGGGCTGCTT 
TTTGAGATAA TAGAGATGCT GCAAGCGGAG GAGACTTCCA GCACCTCTCA GCTGAACGAG 
CTAATGATGG CTTCCCAGAC AACTCTACTC TCTCAGGATC CACCGCTCGT AACCACGGAT 
GTAATCGAGC GCCAAGGGAC GTTCCTGATC AACCTAGAAG GTGGTGATAT TCGCGAAGAG 
TCTTCATATA AAGTAATTGT CATGCCAACT ACCAAAGAAA AATGCCCTCG TTGTTGGAAG 
TACACAGCTG AGTCTTCAGA TACACTCTGC CCCCGGTGCG CAGAAGTCGT CAACGGAAAG 
TAA