NCKAP1 cDNA ORF clone, Canis lupus familiaris(dog)

The following NCKAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCKAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe45070 XM_014110777.2
Latest version!
Canis lupus familiaris NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe31189 XM_535990.6
Latest version!
Canis lupus familiaris NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OCe31189 XM_535990.5 Canis lupus familiaris NCK-associated protein 1 (NCKAP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe45070 XM_014110777.1 Canis lupus familiaris NCK-associated protein 1 (NCKAP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe45070
    Clone ID Related Accession (Same CDS sequence) XM_014110777.1 , XM_014110777.2
    Accession Version XM_014110777.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3303bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nck-associated protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726122.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTTCCCC AGCAAGGACA AGTTTGGAAG GCATGTGGAG ATCCCAAGGC AAAACCATCC 
    TACCTTATTG ACAAAAACCT GGAATCTGCT GTGAAATTCA TAGTCAGAAA GTTCCCTGCT
    GTAGAAACTC GCAACAACAA TCTTGCACAG CTACAGAAAG AAAAATCAGA GATTCTGAAA
    AATCTGGCAT TGTACTATTT CACATTTGTA GATGTTATGG AATTTAAGGA CCATGTTTGT
    GAATTGCTGA ATACTATTGA TGTTTGCCAA GTCTTCTTTG ATATTACTGT AAACTTTGAT
    TTAACAAAGA ACTACTTAGA TTTGATCATA ACCTATACGA CACTAATGAT ACTGCTGTCT
    CGAATTGAAG AAAGGAAGGC AATCATTGGA TTATACAACT ATGCTCACGA AATGACCCAT
    GGAGCAAGTG ACCGAGAATA TCCGCGCCTT GGTCAGATGA TTGTGGATTA TGAAAACCCT
    TTAAAGAAGA TGATGGAAGA ATTTGTACCC CACAGCAAGT CTCTTTCAGA TGCACTGATT
    TCTCTTCAGA TGGTGTATCC TCGAAGGAAT CTTTCAGCTG ACCAGTGGAG AAATGCCCAA
    TTATTGAGTC TCATCAGTGC ACCTAGTACA ATGCTTAATC CTGCACAGTC TGACACTATG
    CCTTGTGAAT ATCTTTCTTT GGATGCAATG GAAAAATGGA TAATCTTTGG CTTCATTTTG
    TGCCATGGGA TTCTAAATAC TGATGCGACA GCTTTGAACC TTTGGAAGCT AGCTCTTCAA
    AGTAGCTCTT GCCTCTCTCT CTTTCGGGAT GAAGTTTTCC ACATTCATAA AGCTGCAGAA
    GACTTATTTG TAAACATACG AGGCTATAAT AAGCGTATTA ATGACATAAG AGAATGCAAA
    GAGGCAGCTG TGTCACATGC TGGTTCAATG CACAGAGAAA GACGCAAATT TTTAAGGTCT
    GCGCTGAAAG AATTGGCTAC TGTCCTCTCT GATCAACCTG GCTTGCTAGG TCCTAAGGCA
    CTTTTTGTTT TTATGGCATT ATCCTTTGCC CGTGATGAAA TTATCTGGCT ACTTCGTCAT
    GCAGATAACA TGCCAAAGAA GAGTGCAGAT GACTTTATAG ACAAGCACAT TGCTGAATTA
    ATATTTTACA TGGAAGAGCT TAGAGCACAT GTAAGGAAAT ACGGACCTGT AATGCAGAGG
    TATTATGTAC AGTACCTTTC TGGCTTTGAT GCTGTTGTTC TCAATGAACT TGTGCAGAAT
    CTTTCTGTTT GCCCTGAAGA TGAATCAATC ATCATGTCCT CTTTTGTTAA CACTATGACT
    TCCTTGAGTG TAAAACAAGT TGAAGATGGG GAAGTATTTG ATTTCAGAGG AATGAGATTA
    GATTGGTTTA GATTACAGGC ATATACTAGT GTTTCTAAGG CTTCACTTAG CCTTGCAGAT
    CACAGAGAAC TTGGAAAGAT GATGAATACA ATAATTTTTC ATACAAAAAT GGTAGATTCT
    TTGGTGGAAA TGTTGGTGGA AACATCAGAT CTCTCCATAT TTTGTTTTTA TAGTCGTGCT
    TTCGAGAAGA TGTTTCAACA GTGTTTGGAG TTACCCTCTC AGTCAAGATA CTCCATTGCA
    TTTCCACTAC TTTGCACTCA TTTTATGAGT TGCACACATG AACTGTGTCC TGAAGAGCGA
    CATCATATTG GAGATCGCAG TCTTTCTTTA TGTAATATGT TCTTGGATGA AATGGCCAAA
    CAAGCTCGAA ATCTCATCAC TGATATTTGC ACAGAACAGT GTACTCTTAG TGACCAGTTG
    TTGCCCAAGC ATTGTGCCAA AACCATTAGT CAAGCAGTGA ATAAGAAGTC AAAAAAACAG
    ACTGGTAAGA AAGGGGAACC TGAAAGGGAA AAGCCAGGAG TTGAGAGTAT GAGGAAAAAC
    AGGCTGGTAG TGACCAACCT TGATAAATTG CACACTGCAC TTTCTGAGTT GTGTTTCTCT
    ATAAATTATG TACCAAACAT GGTGGTTTGG GAACATACTT TTACCCCACG AGAATATTTG
    ACTTCTCATC TGGAAATCCG CTTTACAAAG TCAATTGTTG GAATGACTAT GTATAATCAA
    GCCACACAGG AAATTGCAAA ACCATCAGAG CTTCTAACAA GTGTAAGAGC ATATATGACT
    GTACTACAGT CCATAGAAAA CTATGTGCAA ATTGACATTA CAAGAGTGTT TAACAACGTT
    CTTCTTCAAC AAACACAACA TTTAGACAGT CATGGAGAAC CAACCATCAC AAGTCTATAC
    ACAAATTGGT ATTTGGAAAC TTTGTTAAGA CAAGTCAGCA ATGGTCACAT AGCTTATTTC
    CCTGCAATGA AAGCGTTTGT GAACTTACCT ACAGAAAATG AGTTAACATT CAATGCAGAG
    GAATACTCTG ACATATCAGA AATGAGATCG TTATCAGAGC TCCTAGGCCC ATATGGTATG
    AAGTTCCTAA GTGAGAGCCT TATGTGGCAC ATTTCATCAC AAGTTGCTGA ACTTAAGAAA
    CTTGTGGTGG AGAATGTTGA TGTGCTAACA CAAATGAGGA CCAGCTTTGA CAAACCAGAC
    CAGATGGCCG CACTCTTTAA AAGATTATCA TCTGTCGACA GTGTCTTGAA GAGGATGACA
    ATAATTGGTG TGATTTTATC CTTCCGATCG TTGGCACAAG AAGCACTTAG AGATGTCTTA
    TCTTACCACA TTCCTTTTCT TGTAAGTTCA ATTGAAGATT TCAAGGATCA CATTCCAAGG
    GAAACTGATA TGAAGGTTGC AATGAATGTG TATGAGTTAT CATCAGCTGC TGGATTACCT
    TGCGAGATTG ATCCTGCCTT GGTGGTAGCT CTCTCCTCAC AAAAATCAGA AAACATAAGT
    CCAGAAGAAG AATATAAAAT TGCCTGCCTC CTTATGGTCT TTGTGGCAGT TTCCTTGCCG
    ACACTGGCAA GTAACGTGAT GTCTCAGTAT AGCCCTGCTA TAGAAGGGCA CTGCAACAAC
    ATACATTGCT TGGCCAAAGC CATCAACCAG ATTGCCGCAG CTTTGTTTAC AATTCACAAA
    GGAAGCATTG AAGACCGTCT TAAGGAATTT CTGGCGCTGG CATCTTCCAG TCTACTGAAA
    ATTGGCCAGG AGACAGATAA AACAACAACG AGAAACAGAG AATCTGTGTA TTTACTGCTA
    GATATGATTG TACAGGAATC CCCATTCCTG ACCATGGATC TTTTGGAATC TTGTTTTCCT
    TACGTCTTGC TGAGAAATGC ATACCATGCC GTCTACAAGC AAAGTGTTAC ATCTTCTGCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966252.1
    CDS57..3359
    Translation

    Target ORF information:

    RefSeq Version XM_014110777.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NCK-associated protein 1 (NCKAP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110777.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTTCCCC AGCAAGGACA AGTTTGGAAG GCATGTGGAG ATCCCAAGGC AAAACCATCC 
    TACCTTATTG ACAAAAACCT GGAATCTGCT GTGAAATTCA TAGTCAGAAA GTTCCCTGCT
    GTAGAAACTC GCAACAACAA TCTTGCACAG CTACAGAAAG AAAAATCAGA GATTCTGAAA
    AATCTGGCAT TGTACTATTT CACATTTGTA GATGTTATGG AATTTAAGGA CCATGTTTGT
    GAATTGCTGA ATACTATTGA TGTTTGCCAA GTCTTCTTTG ATATTACTGT AAACTTTGAT
    TTAACAAAGA ACTACTTAGA TTTGATCATA ACCTATACGA CACTAATGAT ACTGCTGTCT
    CGAATTGAAG AAAGGAAGGC AATCATTGGA TTATACAACT ATGCTCACGA AATGACCCAT
    GGAGCAAGTG ACCGAGAATA TCCGCGCCTT GGTCAGATGA TTGTGGATTA TGAAAACCCT
    TTAAAGAAGA TGATGGAAGA ATTTGTACCC CACAGCAAGT CTCTTTCAGA TGCACTGATT
    TCTCTTCAGA TGGTGTATCC TCGAAGGAAT CTTTCAGCTG ACCAGTGGAG AAATGCCCAA
    TTATTGAGTC TCATCAGTGC ACCTAGTACA ATGCTTAATC CTGCACAGTC TGACACTATG
    CCTTGTGAAT ATCTTTCTTT GGATGCAATG GAAAAATGGA TAATCTTTGG CTTCATTTTG
    TGCCATGGGA TTCTAAATAC TGATGCGACA GCTTTGAACC TTTGGAAGCT AGCTCTTCAA
    AGTAGCTCTT GCCTCTCTCT CTTTCGGGAT GAAGTTTTCC ACATTCATAA AGCTGCAGAA
    GACTTATTTG TAAACATACG AGGCTATAAT AAGCGTATTA ATGACATAAG AGAATGCAAA
    GAGGCAGCTG TGTCACATGC TGGTTCAATG CACAGAGAAA GACGCAAATT TTTAAGGTCT
    GCGCTGAAAG AATTGGCTAC TGTCCTCTCT GATCAACCTG GCTTGCTAGG TCCTAAGGCA
    CTTTTTGTTT TTATGGCATT ATCCTTTGCC CGTGATGAAA TTATCTGGCT ACTTCGTCAT
    GCAGATAACA TGCCAAAGAA GAGTGCAGAT GACTTTATAG ACAAGCACAT TGCTGAATTA
    ATATTTTACA TGGAAGAGCT TAGAGCACAT GTAAGGAAAT ACGGACCTGT AATGCAGAGG
    TATTATGTAC AGTACCTTTC TGGCTTTGAT GCTGTTGTTC TCAATGAACT TGTGCAGAAT
    CTTTCTGTTT GCCCTGAAGA TGAATCAATC ATCATGTCCT CTTTTGTTAA CACTATGACT
    TCCTTGAGTG TAAAACAAGT TGAAGATGGG GAAGTATTTG ATTTCAGAGG AATGAGATTA
    GATTGGTTTA GATTACAGGC ATATACTAGT GTTTCTAAGG CTTCACTTAG CCTTGCAGAT
    CACAGAGAAC TTGGAAAGAT GATGAATACA ATAATTTTTC ATACAAAAAT GGTAGATTCT
    TTGGTGGAAA TGTTGGTGGA AACATCAGAT CTCTCCATAT TTTGTTTTTA TAGTCGTGCT
    TTCGAGAAGA TGTTTCAACA GTGTTTGGAG TTACCCTCTC AGTCAAGATA CTCCATTGCA
    TTTCCACTAC TTTGCACTCA TTTTATGAGT TGCACACATG AACTGTGTCC TGAAGAGCGA
    CATCATATTG GAGATCGCAG TCTTTCTTTA TGTAATATGT TCTTGGATGA AATGGCCAAA
    CAAGCTCGAA ATCTCATCAC TGATATTTGC ACAGAACAGT GTACTCTTAG TGACCAGTTG
    TTGCCCAAGC ATTGTGCCAA AACCATTAGT CAAGCAGTGA ATAAGAAGTC AAAAAAACAG
    ACTGGTAAGA AAGGGGAACC TGAAAGGGAA AAGCCAGGAG TTGAGAGTAT GAGGAAAAAC
    AGGCTGGTAG TGACCAACCT TGATAAATTG CACACTGCAC TTTCTGAGTT GTGTTTCTCT
    ATAAATTATG TACCAAACAT GGTGGTTTGG GAACATACTT TTACCCCACG AGAATATTTG
    ACTTCTCATC TGGAAATCCG CTTTACAAAG TCAATTGTTG GAATGACTAT GTATAATCAA
    GCCACACAGG AAATTGCAAA ACCATCAGAG CTTCTAACAA GTGTAAGAGC ATATATGACT
    GTACTACAGT CCATAGAAAA CTATGTGCAA ATTGACATTA CAAGAGTGTT TAACAACGTT
    CTTCTTCAAC AAACACAACA TTTAGACAGT CATGGAGAAC CAACCATCAC AAGTCTATAC
    ACAAATTGGT ATTTGGAAAC TTTGTTAAGA CAAGTCAGCA ATGGTCACAT AGCTTATTTC
    CCTGCAATGA AAGCGTTTGT GAACTTACCT ACAGAAAATG AGTTAACATT CAATGCAGAG
    GAATACTCTG ACATATCAGA AATGAGATCG TTATCAGAGC TCCTAGGCCC ATATGGTATG
    AAGTTCCTAA GTGAGAGCCT TATGTGGCAC ATTTCATCAC AAGTTGCTGA ACTTAAGAAA
    CTTGTGGTGG AGAATGTTGA TGTGCTAACA CAAATGAGGA CCAGCTTTGA CAAACCAGAC
    CAGATGGCCG CACTCTTTAA AAGATTATCA TCTGTCGACA GTGTCTTGAA GAGGATGACA
    ATAATTGGTG TGATTTTATC CTTCCGATCG TTGGCACAAG AAGCACTTAG AGATGTCTTA
    TCTTACCACA TTCCTTTTCT TGTAAGTTCA ATTGAAGATT TCAAGGATCA CATTCCAAGG
    GAAACTGATA TGAAGGTTGC AATGAATGTG TATGAGTTAT CATCAGCTGC TGGATTACCT
    TGCGAGATTG ATCCTGCCTT GGTGGTAGCT CTCTCCTCAC AAAAATCAGA AAACATAAGT
    CCAGAAGAAG AATATAAAAT TGCCTGCCTC CTTATGGTCT TTGTGGCAGT TTCCTTGCCG
    ACACTGGCAA GTAACGTGAT GTCTCAGTAT AGCCCTGCTA TAGAAGGGCA CTGCAACAAC
    ATACATTGCT TGGCCAAAGC CATCAACCAG ATTGCCGCAG CTTTGTTTAC AATTCACAAA
    GGAAGCATTG AAGACCGTCT TAAGGAATTT CTGGCGCTGG CATCTTCCAG TCTACTGAAA
    ATTGGCCAGG AGACAGATAA AACAACAACG AGAAACAGAG AATCTGTGTA TTTACTGCTA
    GATATGATTG TACAGGAATC CCCATTCCTG ACCATGGATC TTTTGGAATC TTGTTTTCCT
    TACGTCTTGC TGAGAAATGC ATACCATGCC GTCTACAAGC AAAGTGTTAC ATCTTCTGCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45070
    Clone ID Related Accession (Same CDS sequence) XM_014110777.1 , XM_014110777.2
    Accession Version XM_014110777.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3303bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nck-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTTCCCC AGCAAGGACA AGTTTGGAAG GCATGTGGAG ATCCCAAGGC AAAACCATCC 
    TACCTTATTG ACAAAAACCT GGAATCTGCT GTGAAATTCA TAGTCAGAAA GTTCCCTGCT
    GTAGAAACTC GCAACAACAA TCTTGCACAG CTACAGAAAG AAAAATCAGA GATTCTGAAA
    AATCTGGCAT TGTACTATTT CACATTTGTA GATGTTATGG AATTTAAGGA CCATGTTTGT
    GAATTGCTGA ATACTATTGA TGTTTGCCAA GTCTTCTTTG ATATTACTGT AAACTTTGAT
    TTAACAAAGA ACTACTTAGA TTTGATCATA ACCTATACGA CACTAATGAT ACTGCTGTCT
    CGAATTGAAG AAAGGAAGGC AATCATTGGA TTATACAACT ATGCTCACGA AATGACCCAT
    GGAGCAAGTG ACCGAGAATA TCCGCGCCTT GGTCAGATGA TTGTGGATTA TGAAAACCCT
    TTAAAGAAGA TGATGGAAGA ATTTGTACCC CACAGCAAGT CTCTTTCAGA TGCACTGATT
    TCTCTTCAGA TGGTGTATCC TCGAAGGAAT CTTTCAGCTG ACCAGTGGAG AAATGCCCAA
    TTATTGAGTC TCATCAGTGC ACCTAGTACA ATGCTTAATC CTGCACAGTC TGACACTATG
    CCTTGTGAAT ATCTTTCTTT GGATGCAATG GAAAAATGGA TAATCTTTGG CTTCATTTTG
    TGCCATGGGA TTCTAAATAC TGATGCGACA GCTTTGAACC TTTGGAAGCT AGCTCTTCAA
    AGTAGCTCTT GCCTCTCTCT CTTTCGGGAT GAAGTTTTCC ACATTCATAA AGCTGCAGAA
    GACTTATTTG TAAACATACG AGGCTATAAT AAGCGTATTA ATGACATAAG AGAATGCAAA
    GAGGCAGCTG TGTCACATGC TGGTTCAATG CACAGAGAAA GACGCAAATT TTTAAGGTCT
    GCGCTGAAAG AATTGGCTAC TGTCCTCTCT GATCAACCTG GCTTGCTAGG TCCTAAGGCA
    CTTTTTGTTT TTATGGCATT ATCCTTTGCC CGTGATGAAA TTATCTGGCT ACTTCGTCAT
    GCAGATAACA TGCCAAAGAA GAGTGCAGAT GACTTTATAG ACAAGCACAT TGCTGAATTA
    ATATTTTACA TGGAAGAGCT TAGAGCACAT GTAAGGAAAT ACGGACCTGT AATGCAGAGG
    TATTATGTAC AGTACCTTTC TGGCTTTGAT GCTGTTGTTC TCAATGAACT TGTGCAGAAT
    CTTTCTGTTT GCCCTGAAGA TGAATCAATC ATCATGTCCT CTTTTGTTAA CACTATGACT
    TCCTTGAGTG TAAAACAAGT TGAAGATGGG GAAGTATTTG ATTTCAGAGG AATGAGATTA
    GATTGGTTTA GATTACAGGC ATATACTAGT GTTTCTAAGG CTTCACTTAG CCTTGCAGAT
    CACAGAGAAC TTGGAAAGAT GATGAATACA ATAATTTTTC ATACAAAAAT GGTAGATTCT
    TTGGTGGAAA TGTTGGTGGA AACATCAGAT CTCTCCATAT TTTGTTTTTA TAGTCGTGCT
    TTCGAGAAGA TGTTTCAACA GTGTTTGGAG TTACCCTCTC AGTCAAGATA CTCCATTGCA
    TTTCCACTAC TTTGCACTCA TTTTATGAGT TGCACACATG AACTGTGTCC TGAAGAGCGA
    CATCATATTG GAGATCGCAG TCTTTCTTTA TGTAATATGT TCTTGGATGA AATGGCCAAA
    CAAGCTCGAA ATCTCATCAC TGATATTTGC ACAGAACAGT GTACTCTTAG TGACCAGTTG
    TTGCCCAAGC ATTGTGCCAA AACCATTAGT CAAGCAGTGA ATAAGAAGTC AAAAAAACAG
    ACTGGTAAGA AAGGGGAACC TGAAAGGGAA AAGCCAGGAG TTGAGAGTAT GAGGAAAAAC
    AGGCTGGTAG TGACCAACCT TGATAAATTG CACACTGCAC TTTCTGAGTT GTGTTTCTCT
    ATAAATTATG TACCAAACAT GGTGGTTTGG GAACATACTT TTACCCCACG AGAATATTTG
    ACTTCTCATC TGGAAATCCG CTTTACAAAG TCAATTGTTG GAATGACTAT GTATAATCAA
    GCCACACAGG AAATTGCAAA ACCATCAGAG CTTCTAACAA GTGTAAGAGC ATATATGACT
    GTACTACAGT CCATAGAAAA CTATGTGCAA ATTGACATTA CAAGAGTGTT TAACAACGTT
    CTTCTTCAAC AAACACAACA TTTAGACAGT CATGGAGAAC CAACCATCAC AAGTCTATAC
    ACAAATTGGT ATTTGGAAAC TTTGTTAAGA CAAGTCAGCA ATGGTCACAT AGCTTATTTC
    CCTGCAATGA AAGCGTTTGT GAACTTACCT ACAGAAAATG AGTTAACATT CAATGCAGAG
    GAATACTCTG ACATATCAGA AATGAGATCG TTATCAGAGC TCCTAGGCCC ATATGGTATG
    AAGTTCCTAA GTGAGAGCCT TATGTGGCAC ATTTCATCAC AAGTTGCTGA ACTTAAGAAA
    CTTGTGGTGG AGAATGTTGA TGTGCTAACA CAAATGAGGA CCAGCTTTGA CAAACCAGAC
    CAGATGGCCG CACTCTTTAA AAGATTATCA TCTGTCGACA GTGTCTTGAA GAGGATGACA
    ATAATTGGTG TGATTTTATC CTTCCGATCG TTGGCACAAG AAGCACTTAG AGATGTCTTA
    TCTTACCACA TTCCTTTTCT TGTAAGTTCA ATTGAAGATT TCAAGGATCA CATTCCAAGG
    GAAACTGATA TGAAGGTTGC AATGAATGTG TATGAGTTAT CATCAGCTGC TGGATTACCT
    TGCGAGATTG ATCCTGCCTT GGTGGTAGCT CTCTCCTCAC AAAAATCAGA AAACATAAGT
    CCAGAAGAAG AATATAAAAT TGCCTGCCTC CTTATGGTCT TTGTGGCAGT TTCCTTGCCG
    ACACTGGCAA GTAACGTGAT GTCTCAGTAT AGCCCTGCTA TAGAAGGGCA CTGCAACAAC
    ATACATTGCT TGGCCAAAGC CATCAACCAG ATTGCCGCAG CTTTGTTTAC AATTCACAAA
    GGAAGCATTG AAGACCGTCT TAAGGAATTT CTGGCGCTGG CATCTTCCAG TCTACTGAAA
    ATTGGCCAGG AGACAGATAA AACAACAACG AGAAACAGAG AATCTGTGTA TTTACTGCTA
    GATATGATTG TACAGGAATC CCCATTCCTG ACCATGGATC TTTTGGAATC TTGTTTTCCT
    TACGTCTTGC TGAGAAATGC ATACCATGCC GTCTACAAGC AAAGTGTTAC ATCTTCTGCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966252.1
    CDS16..3318
    Translation

    Target ORF information:

    RefSeq Version XM_014110777.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110777.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTTCCCC AGCAAGGACA AGTTTGGAAG GCATGTGGAG ATCCCAAGGC AAAACCATCC 
    TACCTTATTG ACAAAAACCT GGAATCTGCT GTGAAATTCA TAGTCAGAAA GTTCCCTGCT
    GTAGAAACTC GCAACAACAA TCTTGCACAG CTACAGAAAG AAAAATCAGA GATTCTGAAA
    AATCTGGCAT TGTACTATTT CACATTTGTA GATGTTATGG AATTTAAGGA CCATGTTTGT
    GAATTGCTGA ATACTATTGA TGTTTGCCAA GTCTTCTTTG ATATTACTGT AAACTTTGAT
    TTAACAAAGA ACTACTTAGA TTTGATCATA ACCTATACGA CACTAATGAT ACTGCTGTCT
    CGAATTGAAG AAAGGAAGGC AATCATTGGA TTATACAACT ATGCTCACGA AATGACCCAT
    GGAGCAAGTG ACCGAGAATA TCCGCGCCTT GGTCAGATGA TTGTGGATTA TGAAAACCCT
    TTAAAGAAGA TGATGGAAGA ATTTGTACCC CACAGCAAGT CTCTTTCAGA TGCACTGATT
    TCTCTTCAGA TGGTGTATCC TCGAAGGAAT CTTTCAGCTG ACCAGTGGAG AAATGCCCAA
    TTATTGAGTC TCATCAGTGC ACCTAGTACA ATGCTTAATC CTGCACAGTC TGACACTATG
    CCTTGTGAAT ATCTTTCTTT GGATGCAATG GAAAAATGGA TAATCTTTGG CTTCATTTTG
    TGCCATGGGA TTCTAAATAC TGATGCGACA GCTTTGAACC TTTGGAAGCT AGCTCTTCAA
    AGTAGCTCTT GCCTCTCTCT CTTTCGGGAT GAAGTTTTCC ACATTCATAA AGCTGCAGAA
    GACTTATTTG TAAACATACG AGGCTATAAT AAGCGTATTA ATGACATAAG AGAATGCAAA
    GAGGCAGCTG TGTCACATGC TGGTTCAATG CACAGAGAAA GACGCAAATT TTTAAGGTCT
    GCGCTGAAAG AATTGGCTAC TGTCCTCTCT GATCAACCTG GCTTGCTAGG TCCTAAGGCA
    CTTTTTGTTT TTATGGCATT ATCCTTTGCC CGTGATGAAA TTATCTGGCT ACTTCGTCAT
    GCAGATAACA TGCCAAAGAA GAGTGCAGAT GACTTTATAG ACAAGCACAT TGCTGAATTA
    ATATTTTACA TGGAAGAGCT TAGAGCACAT GTAAGGAAAT ACGGACCTGT AATGCAGAGG
    TATTATGTAC AGTACCTTTC TGGCTTTGAT GCTGTTGTTC TCAATGAACT TGTGCAGAAT
    CTTTCTGTTT GCCCTGAAGA TGAATCAATC ATCATGTCCT CTTTTGTTAA CACTATGACT
    TCCTTGAGTG TAAAACAAGT TGAAGATGGG GAAGTATTTG ATTTCAGAGG AATGAGATTA
    GATTGGTTTA GATTACAGGC ATATACTAGT GTTTCTAAGG CTTCACTTAG CCTTGCAGAT
    CACAGAGAAC TTGGAAAGAT GATGAATACA ATAATTTTTC ATACAAAAAT GGTAGATTCT
    TTGGTGGAAA TGTTGGTGGA AACATCAGAT CTCTCCATAT TTTGTTTTTA TAGTCGTGCT
    TTCGAGAAGA TGTTTCAACA GTGTTTGGAG TTACCCTCTC AGTCAAGATA CTCCATTGCA
    TTTCCACTAC TTTGCACTCA TTTTATGAGT TGCACACATG AACTGTGTCC TGAAGAGCGA
    CATCATATTG GAGATCGCAG TCTTTCTTTA TGTAATATGT TCTTGGATGA AATGGCCAAA
    CAAGCTCGAA ATCTCATCAC TGATATTTGC ACAGAACAGT GTACTCTTAG TGACCAGTTG
    TTGCCCAAGC ATTGTGCCAA AACCATTAGT CAAGCAGTGA ATAAGAAGTC AAAAAAACAG
    ACTGGTAAGA AAGGGGAACC TGAAAGGGAA AAGCCAGGAG TTGAGAGTAT GAGGAAAAAC
    AGGCTGGTAG TGACCAACCT TGATAAATTG CACACTGCAC TTTCTGAGTT GTGTTTCTCT
    ATAAATTATG TACCAAACAT GGTGGTTTGG GAACATACTT TTACCCCACG AGAATATTTG
    ACTTCTCATC TGGAAATCCG CTTTACAAAG TCAATTGTTG GAATGACTAT GTATAATCAA
    GCCACACAGG AAATTGCAAA ACCATCAGAG CTTCTAACAA GTGTAAGAGC ATATATGACT
    GTACTACAGT CCATAGAAAA CTATGTGCAA ATTGACATTA CAAGAGTGTT TAACAACGTT
    CTTCTTCAAC AAACACAACA TTTAGACAGT CATGGAGAAC CAACCATCAC AAGTCTATAC
    ACAAATTGGT ATTTGGAAAC TTTGTTAAGA CAAGTCAGCA ATGGTCACAT AGCTTATTTC
    CCTGCAATGA AAGCGTTTGT GAACTTACCT ACAGAAAATG AGTTAACATT CAATGCAGAG
    GAATACTCTG ACATATCAGA AATGAGATCG TTATCAGAGC TCCTAGGCCC ATATGGTATG
    AAGTTCCTAA GTGAGAGCCT TATGTGGCAC ATTTCATCAC AAGTTGCTGA ACTTAAGAAA
    CTTGTGGTGG AGAATGTTGA TGTGCTAACA CAAATGAGGA CCAGCTTTGA CAAACCAGAC
    CAGATGGCCG CACTCTTTAA AAGATTATCA TCTGTCGACA GTGTCTTGAA GAGGATGACA
    ATAATTGGTG TGATTTTATC CTTCCGATCG TTGGCACAAG AAGCACTTAG AGATGTCTTA
    TCTTACCACA TTCCTTTTCT TGTAAGTTCA ATTGAAGATT TCAAGGATCA CATTCCAAGG
    GAAACTGATA TGAAGGTTGC AATGAATGTG TATGAGTTAT CATCAGCTGC TGGATTACCT
    TGCGAGATTG ATCCTGCCTT GGTGGTAGCT CTCTCCTCAC AAAAATCAGA AAACATAAGT
    CCAGAAGAAG AATATAAAAT TGCCTGCCTC CTTATGGTCT TTGTGGCAGT TTCCTTGCCG
    ACACTGGCAA GTAACGTGAT GTCTCAGTAT AGCCCTGCTA TAGAAGGGCA CTGCAACAAC
    ATACATTGCT TGGCCAAAGC CATCAACCAG ATTGCCGCAG CTTTGTTTAC AATTCACAAA
    GGAAGCATTG AAGACCGTCT TAAGGAATTT CTGGCGCTGG CATCTTCCAG TCTACTGAAA
    ATTGGCCAGG AGACAGATAA AACAACAACG AGAAACAGAG AATCTGTGTA TTTACTGCTA
    GATATGATTG TACAGGAATC CCCATTCCTG ACCATGGATC TTTTGGAATC TTGTTTTCCT
    TACGTCTTGC TGAGAAATGC ATACCATGCC GTCTACAAGC AAAGTGTTAC ATCTTCTGCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31189
    Clone ID Related Accession (Same CDS sequence) XM_535990.5 , XM_535990.6
    Accession Version XM_535990.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3309bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nck-associated protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726122.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_535990.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTTCCCC AGCAAGGACA AGTTTGGAAG GCATGTGGAG ATCCCAAGGC AAAACCATCC 
    TACCTTATTG ACAAAAACCT GGAATCTGCT GTGAAATTCA TAGTCAGAAA GTTCCCTGCT
    GTAGAAACTC GCAACAACAA TCAACAGCTT GCACAGCTAC AGAAAGAAAA ATCAGAGATT
    CTGAAAAATC TGGCATTGTA CTATTTCACA TTTGTAGATG TTATGGAATT TAAGGACCAT
    GTTTGTGAAT TGCTGAATAC TATTGATGTT TGCCAAGTCT TCTTTGATAT TACTGTAAAC
    TTTGATTTAA CAAAGAACTA CTTAGATTTG ATCATAACCT ATACGACACT AATGATACTG
    CTGTCTCGAA TTGAAGAAAG GAAGGCAATC ATTGGATTAT ACAACTATGC TCACGAAATG
    ACCCATGGAG CAAGTGACCG AGAATATCCG CGCCTTGGTC AGATGATTGT GGATTATGAA
    AACCCTTTAA AGAAGATGAT GGAAGAATTT GTACCCCACA GCAAGTCTCT TTCAGATGCA
    CTGATTTCTC TTCAGATGGT GTATCCTCGA AGGAATCTTT CAGCTGACCA GTGGAGAAAT
    GCCCAATTAT TGAGTCTCAT CAGTGCACCT AGTACAATGC TTAATCCTGC ACAGTCTGAC
    ACTATGCCTT GTGAATATCT TTCTTTGGAT GCAATGGAAA AATGGATAAT CTTTGGCTTC
    ATTTTGTGCC ATGGGATTCT AAATACTGAT GCGACAGCTT TGAACCTTTG GAAGCTAGCT
    CTTCAAAGTA GCTCTTGCCT CTCTCTCTTT CGGGATGAAG TTTTCCACAT TCATAAAGCT
    GCAGAAGACT TATTTGTAAA CATACGAGGC TATAATAAGC GTATTAATGA CATAAGAGAA
    TGCAAAGAGG CAGCTGTGTC ACATGCTGGT TCAATGCACA GAGAAAGACG CAAATTTTTA
    AGGTCTGCGC TGAAAGAATT GGCTACTGTC CTCTCTGATC AACCTGGCTT GCTAGGTCCT
    AAGGCACTTT TTGTTTTTAT GGCATTATCC TTTGCCCGTG ATGAAATTAT CTGGCTACTT
    CGTCATGCAG ATAACATGCC AAAGAAGAGT GCAGATGACT TTATAGACAA GCACATTGCT
    GAATTAATAT TTTACATGGA AGAGCTTAGA GCACATGTAA GGAAATACGG ACCTGTAATG
    CAGAGGTATT ATGTACAGTA CCTTTCTGGC TTTGATGCTG TTGTTCTCAA TGAACTTGTG
    CAGAATCTTT CTGTTTGCCC TGAAGATGAA TCAATCATCA TGTCCTCTTT TGTTAACACT
    ATGACTTCCT TGAGTGTAAA ACAAGTTGAA GATGGGGAAG TATTTGATTT CAGAGGAATG
    AGATTAGATT GGTTTAGATT ACAGGCATAT ACTAGTGTTT CTAAGGCTTC ACTTAGCCTT
    GCAGATCACA GAGAACTTGG AAAGATGATG AATACAATAA TTTTTCATAC AAAAATGGTA
    GATTCTTTGG TGGAAATGTT GGTGGAAACA TCAGATCTCT CCATATTTTG TTTTTATAGT
    CGTGCTTTCG AGAAGATGTT TCAACAGTGT TTGGAGTTAC CCTCTCAGTC AAGATACTCC
    ATTGCATTTC CACTACTTTG CACTCATTTT ATGAGTTGCA CACATGAACT GTGTCCTGAA
    GAGCGACATC ATATTGGAGA TCGCAGTCTT TCTTTATGTA ATATGTTCTT GGATGAAATG
    GCCAAACAAG CTCGAAATCT CATCACTGAT ATTTGCACAG AACAGTGTAC TCTTAGTGAC
    CAGTTGTTGC CCAAGCATTG TGCCAAAACC ATTAGTCAAG CAGTGAATAA GAAGTCAAAA
    AAACAGACTG GTAAGAAAGG GGAACCTGAA AGGGAAAAGC CAGGAGTTGA GAGTATGAGG
    AAAAACAGGC TGGTAGTGAC CAACCTTGAT AAATTGCACA CTGCACTTTC TGAGTTGTGT
    TTCTCTATAA ATTATGTACC AAACATGGTG GTTTGGGAAC ATACTTTTAC CCCACGAGAA
    TATTTGACTT CTCATCTGGA AATCCGCTTT ACAAAGTCAA TTGTTGGAAT GACTATGTAT
    AATCAAGCCA CACAGGAAAT TGCAAAACCA TCAGAGCTTC TAACAAGTGT AAGAGCATAT
    ATGACTGTAC TACAGTCCAT AGAAAACTAT GTGCAAATTG ACATTACAAG AGTGTTTAAC
    AACGTTCTTC TTCAACAAAC ACAACATTTA GACAGTCATG GAGAACCAAC CATCACAAGT
    CTATACACAA ATTGGTATTT GGAAACTTTG TTAAGACAAG TCAGCAATGG TCACATAGCT
    TATTTCCCTG CAATGAAAGC GTTTGTGAAC TTACCTACAG AAAATGAGTT AACATTCAAT
    GCAGAGGAAT ACTCTGACAT ATCAGAAATG AGATCGTTAT CAGAGCTCCT AGGCCCATAT
    GGTATGAAGT TCCTAAGTGA GAGCCTTATG TGGCACATTT CATCACAAGT TGCTGAACTT
    AAGAAACTTG TGGTGGAGAA TGTTGATGTG CTAACACAAA TGAGGACCAG CTTTGACAAA
    CCAGACCAGA TGGCCGCACT CTTTAAAAGA TTATCATCTG TCGACAGTGT CTTGAAGAGG
    ATGACAATAA TTGGTGTGAT TTTATCCTTC CGATCGTTGG CACAAGAAGC ACTTAGAGAT
    GTCTTATCTT ACCACATTCC TTTTCTTGTA AGTTCAATTG AAGATTTCAA GGATCACATT
    CCAAGGGAAA CTGATATGAA GGTTGCAATG AATGTGTATG AGTTATCATC AGCTGCTGGA
    TTACCTTGCG AGATTGATCC TGCCTTGGTG GTAGCTCTCT CCTCACAAAA ATCAGAAAAC
    ATAAGTCCAG AAGAAGAATA TAAAATTGCC TGCCTCCTTA TGGTCTTTGT GGCAGTTTCC
    TTGCCGACAC TGGCAAGTAA CGTGATGTCT CAGTATAGCC CTGCTATAGA AGGGCACTGC
    AACAACATAC ATTGCTTGGC CAAAGCCATC AACCAGATTG CCGCAGCTTT GTTTACAATT
    CACAAAGGAA GCATTGAAGA CCGTCTTAAG GAATTTCTGG CGCTGGCATC TTCCAGTCTA
    CTGAAAATTG GCCAGGAGAC AGATAAAACA ACAACGAGAA ACAGAGAATC TGTGTATTTA
    CTGCTAGATA TGATTGTACA GGAATCCCCA TTCCTGACCA TGGATCTTTT GGAATCTTGT
    TTTCCTTACG TCTTGCTGAG AAATGCATAC CATGCCGTCT ACAAGCAAAG TGTTACATCT
    TCTGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_535990.3
    CDS57..3365
    Translation

    Target ORF information:

    RefSeq Version XM_535990.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NCK-associated protein 1 (NCKAP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_535990.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTTCCCC AGCAAGGACA AGTTTGGAAG GCATGTGGAG ATCCCAAGGC AAAACCATCC 
    TACCTTATTG ACAAAAACCT GGAATCTGCT GTGAAATTCA TAGTCAGAAA GTTCCCTGCT
    GTAGAAACTC GCAACAACAA TCAACAGCTT GCACAGCTAC AGAAAGAAAA ATCAGAGATT
    CTGAAAAATC TGGCATTGTA CTATTTCACA TTTGTAGATG TTATGGAATT TAAGGACCAT
    GTTTGTGAAT TGCTGAATAC TATTGATGTT TGCCAAGTCT TCTTTGATAT TACTGTAAAC
    TTTGATTTAA CAAAGAACTA CTTAGATTTG ATCATAACCT ATACGACACT AATGATACTG
    CTGTCTCGAA TTGAAGAAAG GAAGGCAATC ATTGGATTAT ACAACTATGC TCACGAAATG
    ACCCATGGAG CAAGTGACCG AGAATATCCG CGCCTTGGTC AGATGATTGT GGATTATGAA
    AACCCTTTAA AGAAGATGAT GGAAGAATTT GTACCCCACA GCAAGTCTCT TTCAGATGCA
    CTGATTTCTC TTCAGATGGT GTATCCTCGA AGGAATCTTT CAGCTGACCA GTGGAGAAAT
    GCCCAATTAT TGAGTCTCAT CAGTGCACCT AGTACAATGC TTAATCCTGC ACAGTCTGAC
    ACTATGCCTT GTGAATATCT TTCTTTGGAT GCAATGGAAA AATGGATAAT CTTTGGCTTC
    ATTTTGTGCC ATGGGATTCT AAATACTGAT GCGACAGCTT TGAACCTTTG GAAGCTAGCT
    CTTCAAAGTA GCTCTTGCCT CTCTCTCTTT CGGGATGAAG TTTTCCACAT TCATAAAGCT
    GCAGAAGACT TATTTGTAAA CATACGAGGC TATAATAAGC GTATTAATGA CATAAGAGAA
    TGCAAAGAGG CAGCTGTGTC ACATGCTGGT TCAATGCACA GAGAAAGACG CAAATTTTTA
    AGGTCTGCGC TGAAAGAATT GGCTACTGTC CTCTCTGATC AACCTGGCTT GCTAGGTCCT
    AAGGCACTTT TTGTTTTTAT GGCATTATCC TTTGCCCGTG ATGAAATTAT CTGGCTACTT
    CGTCATGCAG ATAACATGCC AAAGAAGAGT GCAGATGACT TTATAGACAA GCACATTGCT
    GAATTAATAT TTTACATGGA AGAGCTTAGA GCACATGTAA GGAAATACGG ACCTGTAATG
    CAGAGGTATT ATGTACAGTA CCTTTCTGGC TTTGATGCTG TTGTTCTCAA TGAACTTGTG
    CAGAATCTTT CTGTTTGCCC TGAAGATGAA TCAATCATCA TGTCCTCTTT TGTTAACACT
    ATGACTTCCT TGAGTGTAAA ACAAGTTGAA GATGGGGAAG TATTTGATTT CAGAGGAATG
    AGATTAGATT GGTTTAGATT ACAGGCATAT ACTAGTGTTT CTAAGGCTTC ACTTAGCCTT
    GCAGATCACA GAGAACTTGG AAAGATGATG AATACAATAA TTTTTCATAC AAAAATGGTA
    GATTCTTTGG TGGAAATGTT GGTGGAAACA TCAGATCTCT CCATATTTTG TTTTTATAGT
    CGTGCTTTCG AGAAGATGTT TCAACAGTGT TTGGAGTTAC CCTCTCAGTC AAGATACTCC
    ATTGCATTTC CACTACTTTG CACTCATTTT ATGAGTTGCA CACATGAACT GTGTCCTGAA
    GAGCGACATC ATATTGGAGA TCGCAGTCTT TCTTTATGTA ATATGTTCTT GGATGAAATG
    GCCAAACAAG CTCGAAATCT CATCACTGAT ATTTGCACAG AACAGTGTAC TCTTAGTGAC
    CAGTTGTTGC CCAAGCATTG TGCCAAAACC ATTAGTCAAG CAGTGAATAA GAAGTCAAAA
    AAACAGACTG GTAAGAAAGG GGAACCTGAA AGGGAAAAGC CAGGAGTTGA GAGTATGAGG
    AAAAACAGGC TGGTAGTGAC CAACCTTGAT AAATTGCACA CTGCACTTTC TGAGTTGTGT
    TTCTCTATAA ATTATGTACC AAACATGGTG GTTTGGGAAC ATACTTTTAC CCCACGAGAA
    TATTTGACTT CTCATCTGGA AATCCGCTTT ACAAAGTCAA TTGTTGGAAT GACTATGTAT
    AATCAAGCCA CACAGGAAAT TGCAAAACCA TCAGAGCTTC TAACAAGTGT AAGAGCATAT
    ATGACTGTAC TACAGTCCAT AGAAAACTAT GTGCAAATTG ACATTACAAG AGTGTTTAAC
    AACGTTCTTC TTCAACAAAC ACAACATTTA GACAGTCATG GAGAACCAAC CATCACAAGT
    CTATACACAA ATTGGTATTT GGAAACTTTG TTAAGACAAG TCAGCAATGG TCACATAGCT
    TATTTCCCTG CAATGAAAGC GTTTGTGAAC TTACCTACAG AAAATGAGTT AACATTCAAT
    GCAGAGGAAT ACTCTGACAT ATCAGAAATG AGATCGTTAT CAGAGCTCCT AGGCCCATAT
    GGTATGAAGT TCCTAAGTGA GAGCCTTATG TGGCACATTT CATCACAAGT TGCTGAACTT
    AAGAAACTTG TGGTGGAGAA TGTTGATGTG CTAACACAAA TGAGGACCAG CTTTGACAAA
    CCAGACCAGA TGGCCGCACT CTTTAAAAGA TTATCATCTG TCGACAGTGT CTTGAAGAGG
    ATGACAATAA TTGGTGTGAT TTTATCCTTC CGATCGTTGG CACAAGAAGC ACTTAGAGAT
    GTCTTATCTT ACCACATTCC TTTTCTTGTA AGTTCAATTG AAGATTTCAA GGATCACATT
    CCAAGGGAAA CTGATATGAA GGTTGCAATG AATGTGTATG AGTTATCATC AGCTGCTGGA
    TTACCTTGCG AGATTGATCC TGCCTTGGTG GTAGCTCTCT CCTCACAAAA ATCAGAAAAC
    ATAAGTCCAG AAGAAGAATA TAAAATTGCC TGCCTCCTTA TGGTCTTTGT GGCAGTTTCC
    TTGCCGACAC TGGCAAGTAA CGTGATGTCT CAGTATAGCC CTGCTATAGA AGGGCACTGC
    AACAACATAC ATTGCTTGGC CAAAGCCATC AACCAGATTG CCGCAGCTTT GTTTACAATT
    CACAAAGGAA GCATTGAAGA CCGTCTTAAG GAATTTCTGG CGCTGGCATC TTCCAGTCTA
    CTGAAAATTG GCCAGGAGAC AGATAAAACA ACAACGAGAA ACAGAGAATC TGTGTATTTA
    CTGCTAGATA TGATTGTACA GGAATCCCCA TTCCTGACCA TGGATCTTTT GGAATCTTGT
    TTTCCTTACG TCTTGCTGAG AAATGCATAC CATGCCGTCT ACAAGCAAAG TGTTACATCT
    TCTGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31189
    Clone ID Related Accession (Same CDS sequence) XM_535990.5 , XM_535990.6
    Accession Version XM_535990.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3309bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nck-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_535990.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTTCCCC AGCAAGGACA AGTTTGGAAG GCATGTGGAG ATCCCAAGGC AAAACCATCC 
    TACCTTATTG ACAAAAACCT GGAATCTGCT GTGAAATTCA TAGTCAGAAA GTTCCCTGCT
    GTAGAAACTC GCAACAACAA TCAACAGCTT GCACAGCTAC AGAAAGAAAA ATCAGAGATT
    CTGAAAAATC TGGCATTGTA CTATTTCACA TTTGTAGATG TTATGGAATT TAAGGACCAT
    GTTTGTGAAT TGCTGAATAC TATTGATGTT TGCCAAGTCT TCTTTGATAT TACTGTAAAC
    TTTGATTTAA CAAAGAACTA CTTAGATTTG ATCATAACCT ATACGACACT AATGATACTG
    CTGTCTCGAA TTGAAGAAAG GAAGGCAATC ATTGGATTAT ACAACTATGC TCACGAAATG
    ACCCATGGAG CAAGTGACCG AGAATATCCG CGCCTTGGTC AGATGATTGT GGATTATGAA
    AACCCTTTAA AGAAGATGAT GGAAGAATTT GTACCCCACA GCAAGTCTCT TTCAGATGCA
    CTGATTTCTC TTCAGATGGT GTATCCTCGA AGGAATCTTT CAGCTGACCA GTGGAGAAAT
    GCCCAATTAT TGAGTCTCAT CAGTGCACCT AGTACAATGC TTAATCCTGC ACAGTCTGAC
    ACTATGCCTT GTGAATATCT TTCTTTGGAT GCAATGGAAA AATGGATAAT CTTTGGCTTC
    ATTTTGTGCC ATGGGATTCT AAATACTGAT GCGACAGCTT TGAACCTTTG GAAGCTAGCT
    CTTCAAAGTA GCTCTTGCCT CTCTCTCTTT CGGGATGAAG TTTTCCACAT TCATAAAGCT
    GCAGAAGACT TATTTGTAAA CATACGAGGC TATAATAAGC GTATTAATGA CATAAGAGAA
    TGCAAAGAGG CAGCTGTGTC ACATGCTGGT TCAATGCACA GAGAAAGACG CAAATTTTTA
    AGGTCTGCGC TGAAAGAATT GGCTACTGTC CTCTCTGATC AACCTGGCTT GCTAGGTCCT
    AAGGCACTTT TTGTTTTTAT GGCATTATCC TTTGCCCGTG ATGAAATTAT CTGGCTACTT
    CGTCATGCAG ATAACATGCC AAAGAAGAGT GCAGATGACT TTATAGACAA GCACATTGCT
    GAATTAATAT TTTACATGGA AGAGCTTAGA GCACATGTAA GGAAATACGG ACCTGTAATG
    CAGAGGTATT ATGTACAGTA CCTTTCTGGC TTTGATGCTG TTGTTCTCAA TGAACTTGTG
    CAGAATCTTT CTGTTTGCCC TGAAGATGAA TCAATCATCA TGTCCTCTTT TGTTAACACT
    ATGACTTCCT TGAGTGTAAA ACAAGTTGAA GATGGGGAAG TATTTGATTT CAGAGGAATG
    AGATTAGATT GGTTTAGATT ACAGGCATAT ACTAGTGTTT CTAAGGCTTC ACTTAGCCTT
    GCAGATCACA GAGAACTTGG AAAGATGATG AATACAATAA TTTTTCATAC AAAAATGGTA
    GATTCTTTGG TGGAAATGTT GGTGGAAACA TCAGATCTCT CCATATTTTG TTTTTATAGT
    CGTGCTTTCG AGAAGATGTT TCAACAGTGT TTGGAGTTAC CCTCTCAGTC AAGATACTCC
    ATTGCATTTC CACTACTTTG CACTCATTTT ATGAGTTGCA CACATGAACT GTGTCCTGAA
    GAGCGACATC ATATTGGAGA TCGCAGTCTT TCTTTATGTA ATATGTTCTT GGATGAAATG
    GCCAAACAAG CTCGAAATCT CATCACTGAT ATTTGCACAG AACAGTGTAC TCTTAGTGAC
    CAGTTGTTGC CCAAGCATTG TGCCAAAACC ATTAGTCAAG CAGTGAATAA GAAGTCAAAA
    AAACAGACTG GTAAGAAAGG GGAACCTGAA AGGGAAAAGC CAGGAGTTGA GAGTATGAGG
    AAAAACAGGC TGGTAGTGAC CAACCTTGAT AAATTGCACA CTGCACTTTC TGAGTTGTGT
    TTCTCTATAA ATTATGTACC AAACATGGTG GTTTGGGAAC ATACTTTTAC CCCACGAGAA
    TATTTGACTT CTCATCTGGA AATCCGCTTT ACAAAGTCAA TTGTTGGAAT GACTATGTAT
    AATCAAGCCA CACAGGAAAT TGCAAAACCA TCAGAGCTTC TAACAAGTGT AAGAGCATAT
    ATGACTGTAC TACAGTCCAT AGAAAACTAT GTGCAAATTG ACATTACAAG AGTGTTTAAC
    AACGTTCTTC TTCAACAAAC ACAACATTTA GACAGTCATG GAGAACCAAC CATCACAAGT
    CTATACACAA ATTGGTATTT GGAAACTTTG TTAAGACAAG TCAGCAATGG TCACATAGCT
    TATTTCCCTG CAATGAAAGC GTTTGTGAAC TTACCTACAG AAAATGAGTT AACATTCAAT
    GCAGAGGAAT ACTCTGACAT ATCAGAAATG AGATCGTTAT CAGAGCTCCT AGGCCCATAT
    GGTATGAAGT TCCTAAGTGA GAGCCTTATG TGGCACATTT CATCACAAGT TGCTGAACTT
    AAGAAACTTG TGGTGGAGAA TGTTGATGTG CTAACACAAA TGAGGACCAG CTTTGACAAA
    CCAGACCAGA TGGCCGCACT CTTTAAAAGA TTATCATCTG TCGACAGTGT CTTGAAGAGG
    ATGACAATAA TTGGTGTGAT TTTATCCTTC CGATCGTTGG CACAAGAAGC ACTTAGAGAT
    GTCTTATCTT ACCACATTCC TTTTCTTGTA AGTTCAATTG AAGATTTCAA GGATCACATT
    CCAAGGGAAA CTGATATGAA GGTTGCAATG AATGTGTATG AGTTATCATC AGCTGCTGGA
    TTACCTTGCG AGATTGATCC TGCCTTGGTG GTAGCTCTCT CCTCACAAAA ATCAGAAAAC
    ATAAGTCCAG AAGAAGAATA TAAAATTGCC TGCCTCCTTA TGGTCTTTGT GGCAGTTTCC
    TTGCCGACAC TGGCAAGTAA CGTGATGTCT CAGTATAGCC CTGCTATAGA AGGGCACTGC
    AACAACATAC ATTGCTTGGC CAAAGCCATC AACCAGATTG CCGCAGCTTT GTTTACAATT
    CACAAAGGAA GCATTGAAGA CCGTCTTAAG GAATTTCTGG CGCTGGCATC TTCCAGTCTA
    CTGAAAATTG GCCAGGAGAC AGATAAAACA ACAACGAGAA ACAGAGAATC TGTGTATTTA
    CTGCTAGATA TGATTGTACA GGAATCCCCA TTCCTGACCA TGGATCTTTT GGAATCTTGT
    TTTCCTTACG TCTTGCTGAG AAATGCATAC CATGCCGTCT ACAAGCAAAG TGTTACATCT
    TCTGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_535990.3
    CDS16..3324
    Translation

    Target ORF information:

    RefSeq Version XM_535990.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_535990.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTTCCCC AGCAAGGACA AGTTTGGAAG GCATGTGGAG ATCCCAAGGC AAAACCATCC 
    TACCTTATTG ACAAAAACCT GGAATCTGCT GTGAAATTCA TAGTCAGAAA GTTCCCTGCT
    GTAGAAACTC GCAACAACAA TCAACAGCTT GCACAGCTAC AGAAAGAAAA ATCAGAGATT
    CTGAAAAATC TGGCATTGTA CTATTTCACA TTTGTAGATG TTATGGAATT TAAGGACCAT
    GTTTGTGAAT TGCTGAATAC TATTGATGTT TGCCAAGTCT TCTTTGATAT TACTGTAAAC
    TTTGATTTAA CAAAGAACTA CTTAGATTTG ATCATAACCT ATACGACACT AATGATACTG
    CTGTCTCGAA TTGAAGAAAG GAAGGCAATC ATTGGATTAT ACAACTATGC TCACGAAATG
    ACCCATGGAG CAAGTGACCG AGAATATCCG CGCCTTGGTC AGATGATTGT GGATTATGAA
    AACCCTTTAA AGAAGATGAT GGAAGAATTT GTACCCCACA GCAAGTCTCT TTCAGATGCA
    CTGATTTCTC TTCAGATGGT GTATCCTCGA AGGAATCTTT CAGCTGACCA GTGGAGAAAT
    GCCCAATTAT TGAGTCTCAT CAGTGCACCT AGTACAATGC TTAATCCTGC ACAGTCTGAC
    ACTATGCCTT GTGAATATCT TTCTTTGGAT GCAATGGAAA AATGGATAAT CTTTGGCTTC
    ATTTTGTGCC ATGGGATTCT AAATACTGAT GCGACAGCTT TGAACCTTTG GAAGCTAGCT
    CTTCAAAGTA GCTCTTGCCT CTCTCTCTTT CGGGATGAAG TTTTCCACAT TCATAAAGCT
    GCAGAAGACT TATTTGTAAA CATACGAGGC TATAATAAGC GTATTAATGA CATAAGAGAA
    TGCAAAGAGG CAGCTGTGTC ACATGCTGGT TCAATGCACA GAGAAAGACG CAAATTTTTA
    AGGTCTGCGC TGAAAGAATT GGCTACTGTC CTCTCTGATC AACCTGGCTT GCTAGGTCCT
    AAGGCACTTT TTGTTTTTAT GGCATTATCC TTTGCCCGTG ATGAAATTAT CTGGCTACTT
    CGTCATGCAG ATAACATGCC AAAGAAGAGT GCAGATGACT TTATAGACAA GCACATTGCT
    GAATTAATAT TTTACATGGA AGAGCTTAGA GCACATGTAA GGAAATACGG ACCTGTAATG
    CAGAGGTATT ATGTACAGTA CCTTTCTGGC TTTGATGCTG TTGTTCTCAA TGAACTTGTG
    CAGAATCTTT CTGTTTGCCC TGAAGATGAA TCAATCATCA TGTCCTCTTT TGTTAACACT
    ATGACTTCCT TGAGTGTAAA ACAAGTTGAA GATGGGGAAG TATTTGATTT CAGAGGAATG
    AGATTAGATT GGTTTAGATT ACAGGCATAT ACTAGTGTTT CTAAGGCTTC ACTTAGCCTT
    GCAGATCACA GAGAACTTGG AAAGATGATG AATACAATAA TTTTTCATAC AAAAATGGTA
    GATTCTTTGG TGGAAATGTT GGTGGAAACA TCAGATCTCT CCATATTTTG TTTTTATAGT
    CGTGCTTTCG AGAAGATGTT TCAACAGTGT TTGGAGTTAC CCTCTCAGTC AAGATACTCC
    ATTGCATTTC CACTACTTTG CACTCATTTT ATGAGTTGCA CACATGAACT GTGTCCTGAA
    GAGCGACATC ATATTGGAGA TCGCAGTCTT TCTTTATGTA ATATGTTCTT GGATGAAATG
    GCCAAACAAG CTCGAAATCT CATCACTGAT ATTTGCACAG AACAGTGTAC TCTTAGTGAC
    CAGTTGTTGC CCAAGCATTG TGCCAAAACC ATTAGTCAAG CAGTGAATAA GAAGTCAAAA
    AAACAGACTG GTAAGAAAGG GGAACCTGAA AGGGAAAAGC CAGGAGTTGA GAGTATGAGG
    AAAAACAGGC TGGTAGTGAC CAACCTTGAT AAATTGCACA CTGCACTTTC TGAGTTGTGT
    TTCTCTATAA ATTATGTACC AAACATGGTG GTTTGGGAAC ATACTTTTAC CCCACGAGAA
    TATTTGACTT CTCATCTGGA AATCCGCTTT ACAAAGTCAA TTGTTGGAAT GACTATGTAT
    AATCAAGCCA CACAGGAAAT TGCAAAACCA TCAGAGCTTC TAACAAGTGT AAGAGCATAT
    ATGACTGTAC TACAGTCCAT AGAAAACTAT GTGCAAATTG ACATTACAAG AGTGTTTAAC
    AACGTTCTTC TTCAACAAAC ACAACATTTA GACAGTCATG GAGAACCAAC CATCACAAGT
    CTATACACAA ATTGGTATTT GGAAACTTTG TTAAGACAAG TCAGCAATGG TCACATAGCT
    TATTTCCCTG CAATGAAAGC GTTTGTGAAC TTACCTACAG AAAATGAGTT AACATTCAAT
    GCAGAGGAAT ACTCTGACAT ATCAGAAATG AGATCGTTAT CAGAGCTCCT AGGCCCATAT
    GGTATGAAGT TCCTAAGTGA GAGCCTTATG TGGCACATTT CATCACAAGT TGCTGAACTT
    AAGAAACTTG TGGTGGAGAA TGTTGATGTG CTAACACAAA TGAGGACCAG CTTTGACAAA
    CCAGACCAGA TGGCCGCACT CTTTAAAAGA TTATCATCTG TCGACAGTGT CTTGAAGAGG
    ATGACAATAA TTGGTGTGAT TTTATCCTTC CGATCGTTGG CACAAGAAGC ACTTAGAGAT
    GTCTTATCTT ACCACATTCC TTTTCTTGTA AGTTCAATTG AAGATTTCAA GGATCACATT
    CCAAGGGAAA CTGATATGAA GGTTGCAATG AATGTGTATG AGTTATCATC AGCTGCTGGA
    TTACCTTGCG AGATTGATCC TGCCTTGGTG GTAGCTCTCT CCTCACAAAA ATCAGAAAAC
    ATAAGTCCAG AAGAAGAATA TAAAATTGCC TGCCTCCTTA TGGTCTTTGT GGCAGTTTCC
    TTGCCGACAC TGGCAAGTAA CGTGATGTCT CAGTATAGCC CTGCTATAGA AGGGCACTGC
    AACAACATAC ATTGCTTGGC CAAAGCCATC AACCAGATTG CCGCAGCTTT GTTTACAATT
    CACAAAGGAA GCATTGAAGA CCGTCTTAAG GAATTTCTGG CGCTGGCATC TTCCAGTCTA
    CTGAAAATTG GCCAGGAGAC AGATAAAACA ACAACGAGAA ACAGAGAATC TGTGTATTTA
    CTGCTAGATA TGATTGTACA GGAATCCCCA TTCCTGACCA TGGATCTTTT GGAATCTTGT
    TTTCCTTACG TCTTGCTGAG AAATGCATAC CATGCCGTCT ACAAGCAAAG TGTTACATCT
    TCTGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.