MARCH7 cDNA ORF clone, Canis lupus familiaris(dog)

The following MARCH7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MARCH7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe284748 XM_022414866.1
Latest version!
Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCe284749 XM_022414867.1
Latest version!
Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OCe284750 XM_022414868.1
Latest version!
Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OCe284751 XM_022414869.1
Latest version!
Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OCe284752 XM_022414870.1
Latest version!
Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OCe31002 XM_005640222.2
Latest version!
Canis lupus familiaris membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase (MARCH7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCe31002 XM_005640221.2
Latest version!
Canis lupus familiaris membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase (MARCH7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCe31003 XM_843631.5
Latest version!
Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCe31003 XM_022414865.1
Latest version!
Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCe31004 XM_005640224.3
Latest version!
Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCe31005 XM_005640225.3
Latest version!
Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OCe31005 XM_005640225.2 Canis lupus familiaris membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase (MARCH7), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCe31003 XM_843631.4 Canis lupus familiaris membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase (MARCH7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OCe31004 XM_005640224.2 Canis lupus familiaris membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase (MARCH7), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe284748
    Clone ID Related Accession (Same CDS sequence) XM_022414866.1
    Accession Version XM_022414866.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MARCH7 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGTTCAAA TGTACCATGA TCATCTTATC TCTTTGTTAA CATCCACATC AGCATCAGCA 
    TCTGCATCAC CATTTCAATC TACATGGTAT AGTGAATCTG AGATAACTCA GGGAGCACGC
    TCAAGATCAC AAAACCAACA ACGGGATCAT GATTCAAAAA GACCTAAACT TTCCTGTACA
    AATTGTACAT CTACCTCAGT CGGGAGAAAT ATTGGACATG GTTTAAATGC AGTACCAGAT
    TCTTCTTGGA GGCATAATCA AGTTCCTAGA TCTTCATCAA TGGTACTTGG ATCATTTGGA
    ACTGACTTAA TGAGAGAGAG AAGAGATTTA GAGAGAAGAG CAGATTCTTC CATTAGTAAT
    CTTATGGATT ATAGTCACCG AAGTGGTGAT TTCACAACTT CATCATATGG TCAAGACAGA
    GTTCCTTCTT CATATTCACA GGGAGCAAGA CCAAAAGATA ACTCAGTGAG CACTTTACAG
    TTGAATACAT CATCCACAAA TCACCAAATG CCTTCTGAAC ATCATACCAT ACCATGTTCT
    AGGGACAACA GTAGAAATTC GCTAAGATCA AATTTTTCTC CAAGGGAATT GGAATCTCCA
    CAAAGCAGTA CACAGCCTGG ATTTTCTTAT ATATCAAATA GAGATGAAAC CTCAACCATA
    AGCAGTTCAG ATAGGGTTGG TTCATCTCAG AGATCATTTC AAGAATCTTC TGACAATGAA
    AGTAGACGTT CGACTAGGAG ATTGCTATCA CGTATAGCTT CTAGTATGTC ATCTACCTTT
    TTTTCTCGAA GATCTAGCCA GGATTCCTTG AATACAAGAT CACTGAGTTC TGAAAATTCT
    TACGTGTCTC CAAGAATCTT GACCGCTTCA CAGTCTCGTA GCAATGCAGC ATCAACTTCC
    GATGTTCCTG ATAGTAGAGC ATCTGAAGCT TCTCAGGGAT TTCGATTTCT TAGGCGAAGA
    TGGGGTTTGT CATCTCTTAG CCAAAATCAT AGCTCTGAGC CAGATTCAGA AAATTTTAAC
    CAAGAATCTG AAGGTAGAAA TACAGGACCG TGGTTATCTT CCTCACTTAG AAATAGATGC
    ACACCTTTGT TCTCCAGAAG GAGACGAGAG GGACGAGATG AATCTTCAAG GATACCTACC
    TCTGATATAC CATCTAGATC TCATCATATT TTTAGAAGAG AATCAAATGA AGTGGTTCAC
    CTAGAAGCAC AGAGTGATCC CCTTGGGGCT ACTGCCAACA GAGCACAAGC ATCTGCATCA
    TCAAGTAACG CTGCTACAGG TGGCTCCCCA TCAGATTCAG CCCACAGTGG AAGAAATACA
    GGAATAACAG GAATCCTTCC TGGTTCCTTA TTTCGATTTG CAGTCCCCCC AGCACTTGGA
    AATAATCTGA CCGACAATGT CATGATTACT GTAGATATTA TTCCTTCAGG GTGGAGTTCA
    TCTGATGGAA AAAGTGATAA AACTAAAAGT GCACCTTCAA GAGATCCAGA AAGACTGCAG
    AAAATAAAAG AGAGCCTCCT TTTAGAGGAT TCTGAAGAAG AAGAAGGTGA CTTATGTAGG
    ATTTGTCAGA TGGCCGCTGC ATCGTCATCT AATTTACTGA TAGAGCCATG CAAGTGCACA
    GGAAGTTTGC AGTATGTCCA CCAAGAATGT ATGAAAAAGT GGCTACAGGC CAAAATTAAC
    TCCGGTTCTT CATTAGAGGC TGTAACCACC TGTGAACTCT GTAAAGAGAA GTTGCAGCTT
    AACCTGGAGG ATTTTGATAT TCATGAACTA CATAGAGCTC ATGCAAATGA ACAAGCTGAG
    TATGAGTTTA TCAGCTCTGG TCTCTACCTA GTTGTGTTAT TGCACTTGTG CGAACAAAGC
    TTTTCTGATA TGATGGGAAA TACAAATGAA CCAAGCACAC GTGTCCGATT TATTAACCTT
    GCAAGAACTC TTCAGGCACA TATGGAAGAT CTCGAAACTT CAGAGGATGA TTCCGAAGAA
    GATGGAGACC ATAACAGAAC ATTTGATATT GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270574.1
    CDS97..2112
    Translation

    Target ORF information:

    RefSeq Version XM_022414866.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414866.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGTTCAAA TGTACCATGA TCATCTTATC TCTTTGTTAA CATCCACATC AGCATCAGCA 
    TCTGCATCAC CATTTCAATC TACATGGTAT AGTGAATCTG AGATAACTCA GGGAGCACGC
    TCAAGATCAC AAAACCAACA ACGGGATCAT GATTCAAAAA GACCTAAACT TTCCTGTACA
    AATTGTACAT CTACCTCAGT CGGGAGAAAT ATTGGACATG GTTTAAATGC AGTACCAGAT
    TCTTCTTGGA GGCATAATCA AGTTCCTAGA TCTTCATCAA TGGTACTTGG ATCATTTGGA
    ACTGACTTAA TGAGAGAGAG AAGAGATTTA GAGAGAAGAG CAGATTCTTC CATTAGTAAT
    CTTATGGATT ATAGTCACCG AAGTGGTGAT TTCACAACTT CATCATATGG TCAAGACAGA
    GTTCCTTCTT CATATTCACA GGGAGCAAGA CCAAAAGATA ACTCAGTGAG CACTTTACAG
    TTGAATACAT CATCCACAAA TCACCAAATG CCTTCTGAAC ATCATACCAT ACCATGTTCT
    AGGGACAACA GTAGAAATTC GCTAAGATCA AATTTTTCTC CAAGGGAATT GGAATCTCCA
    CAAAGCAGTA CACAGCCTGG ATTTTCTTAT ATATCAAATA GAGATGAAAC CTCAACCATA
    AGCAGTTCAG ATAGGGTTGG TTCATCTCAG AGATCATTTC AAGAATCTTC TGACAATGAA
    AGTAGACGTT CGACTAGGAG ATTGCTATCA CGTATAGCTT CTAGTATGTC ATCTACCTTT
    TTTTCTCGAA GATCTAGCCA GGATTCCTTG AATACAAGAT CACTGAGTTC TGAAAATTCT
    TACGTGTCTC CAAGAATCTT GACCGCTTCA CAGTCTCGTA GCAATGCAGC ATCAACTTCC
    GATGTTCCTG ATAGTAGAGC ATCTGAAGCT TCTCAGGGAT TTCGATTTCT TAGGCGAAGA
    TGGGGTTTGT CATCTCTTAG CCAAAATCAT AGCTCTGAGC CAGATTCAGA AAATTTTAAC
    CAAGAATCTG AAGGTAGAAA TACAGGACCG TGGTTATCTT CCTCACTTAG AAATAGATGC
    ACACCTTTGT TCTCCAGAAG GAGACGAGAG GGACGAGATG AATCTTCAAG GATACCTACC
    TCTGATATAC CATCTAGATC TCATCATATT TTTAGAAGAG AATCAAATGA AGTGGTTCAC
    CTAGAAGCAC AGAGTGATCC CCTTGGGGCT ACTGCCAACA GAGCACAAGC ATCTGCATCA
    TCAAGTAACG CTGCTACAGG TGGCTCCCCA TCAGATTCAG CCCACAGTGG AAGAAATACA
    GGAATAACAG GAATCCTTCC TGGTTCCTTA TTTCGATTTG CAGTCCCCCC AGCACTTGGA
    AATAATCTGA CCGACAATGT CATGATTACT GTAGATATTA TTCCTTCAGG GTGGAGTTCA
    TCTGATGGAA AAAGTGATAA AACTAAAAGT GCACCTTCAA GAGATCCAGA AAGACTGCAG
    AAAATAAAAG AGAGCCTCCT TTTAGAGGAT TCTGAAGAAG AAGAAGGTGA CTTATGTAGG
    ATTTGTCAGA TGGCCGCTGC ATCGTCATCT AATTTACTGA TAGAGCCATG CAAGTGCACA
    GGAAGTTTGC AGTATGTCCA CCAAGAATGT ATGAAAAAGT GGCTACAGGC CAAAATTAAC
    TCCGGTTCTT CATTAGAGGC TGTAACCACC TGTGAACTCT GTAAAGAGAA GTTGCAGCTT
    AACCTGGAGG ATTTTGATAT TCATGAACTA CATAGAGCTC ATGCAAATGA ACAAGCTGAG
    TATGAGTTTA TCAGCTCTGG TCTCTACCTA GTTGTGTTAT TGCACTTGTG CGAACAAAGC
    TTTTCTGATA TGATGGGAAA TACAAATGAA CCAAGCACAC GTGTCCGATT TATTAACCTT
    GCAAGAACTC TTCAGGCACA TATGGAAGAT CTCGAAACTT CAGAGGATGA TTCCGAAGAA
    GATGGAGACC ATAACAGAAC ATTTGATATT GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284749
    Clone ID Related Accession (Same CDS sequence) XM_022414867.1
    Accession Version XM_022414867.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MARCH7 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TGGTCAAGAC
    AGAGTTCCTT CTTCATATTC ACAGGGAGCA AGACCAAAAG ATAACTCAGT GAGCACTTTA
    CAGTTGAATA CATCATCCAC AAATCACCAA ATGCCTTCTG AACATCATAC CATACCATGT
    TCTAGGGACA ACAGTAGAAA TTCGCTAAGA TCAAATTTTT CTCCAAGGGA ATTGGAATCT
    CCACAAAGCA GTACACAGCC TGGATTTTCT TATATATCAA ATAGAGATGA AACCTCAACC
    ATAAGCAGTT CAGATAGGGT TGGTTCATCT CAGAGATCAT TTCAAGAATC TTCTGACAAT
    GAAAGTAGAC GTTCGACTAG GAGATTGCTA TCACGTATAG CTTCTAGTAT GTCATCTACC
    TTTTTTTCTC GAAGATCTAG CCAGGATTCC TTGAATACAA GATCACTGAG TTCTGAAAAT
    TCTTACGTGT CTCCAAGAAT CTTGACCGCT TCACAGTCTC GTAGCAATGC AGCATCAACT
    TCCGATGTTC CTGATAGTAG AGCATCTGAA GCTTCTCAGG GATTTCGATT TCTTAGGCGA
    AGATGGGGTT TGTCATCTCT TAGCCAAAAT CATAGCTCTG AGCCAGATTC AGAAAATTTT
    AACCAAGAAT CTGAAGGTAG AAATACAGGA CCGTGGTTAT CTTCCTCACT TAGAAATAGA
    TGCACACCTT TGTTCTCCAG AAGGAGACGA GAGGGACGAG ATGAATCTTC AAGGATACCT
    ACCTCTGATA TACCATCTAG ATCTCATCAT ATTTTTAGAA GAGAATCAAA TGAAGTGGTT
    CACCTAGAAG CACAGAGTGA TCCCCTTGGG GCTACTGCCA ACAGAGCACA AGCATCTGCA
    TCATCAAGTA ACGCTGCTAC AGGTGGCTCC CCATCAGATT CAGCCCACAG TGGAAGAAAT
    ACAGGAATAA CAGGAATCCT TCCTGGTTCC TTATTTCGAT TTGCAGTCCC CCCAGCACTT
    GGAAATAATC TGACCGACAA TGTCATGATT ACTGTAGATA TTATTCCTTC AGGGTGGAGT
    TCATCTGATG GAAAAAGTGA TAAAACTAAA AGTGCACCTT CAAGAGATCC AGAAAGACTG
    CAGAAAATAA AAGAGAGCCT CCTTTTAGAG GATTCTGAAG AAGAAGAAGG TGACTTATGT
    AGGATTTGTC AGATGGCCGC TGCATCGTCA TCTAATTTAC TGATAGAGCC ATGCAAGTGC
    ACAGGAAGTT TGCAGTATGT CCACCAAGAA TGTATGAAAA AGTGGCTACA GGCCAAAATT
    AACTCCGGTT CTTCATTAGA GGCTGTAACC ACCTGTGAAC TCTGTAAAGA GAAGTTGCAG
    CTTAACCTGG AGGATTTTGA TATTCATGAA CTACATAGAG CTCATGCAAA TGAACAAGCT
    GAGTATGAGT TTATCAGCTC TGGTCTCTAC CTAGTTGTGT TATTGCACTT GTGCGAACAA
    AGCTTTTCTG ATATGATGGG AAATACAAAT GAACCAAGCA CACGTGTCCG ATTTATTAAC
    CTTGCAAGAA CTCTTCAGGC ACATATGGAA GATCTCGAAA CTTCAGAGGA TGATTCCGAA
    GAAGATGGAG ACCATAACAG AACATTTGAT ATTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270575.1
    CDS193..2151
    Translation

    Target ORF information:

    RefSeq Version XM_022414867.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414867.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TGGTCAAGAC
    AGAGTTCCTT CTTCATATTC ACAGGGAGCA AGACCAAAAG ATAACTCAGT GAGCACTTTA
    CAGTTGAATA CATCATCCAC AAATCACCAA ATGCCTTCTG AACATCATAC CATACCATGT
    TCTAGGGACA ACAGTAGAAA TTCGCTAAGA TCAAATTTTT CTCCAAGGGA ATTGGAATCT
    CCACAAAGCA GTACACAGCC TGGATTTTCT TATATATCAA ATAGAGATGA AACCTCAACC
    ATAAGCAGTT CAGATAGGGT TGGTTCATCT CAGAGATCAT TTCAAGAATC TTCTGACAAT
    GAAAGTAGAC GTTCGACTAG GAGATTGCTA TCACGTATAG CTTCTAGTAT GTCATCTACC
    TTTTTTTCTC GAAGATCTAG CCAGGATTCC TTGAATACAA GATCACTGAG TTCTGAAAAT
    TCTTACGTGT CTCCAAGAAT CTTGACCGCT TCACAGTCTC GTAGCAATGC AGCATCAACT
    TCCGATGTTC CTGATAGTAG AGCATCTGAA GCTTCTCAGG GATTTCGATT TCTTAGGCGA
    AGATGGGGTT TGTCATCTCT TAGCCAAAAT CATAGCTCTG AGCCAGATTC AGAAAATTTT
    AACCAAGAAT CTGAAGGTAG AAATACAGGA CCGTGGTTAT CTTCCTCACT TAGAAATAGA
    TGCACACCTT TGTTCTCCAG AAGGAGACGA GAGGGACGAG ATGAATCTTC AAGGATACCT
    ACCTCTGATA TACCATCTAG ATCTCATCAT ATTTTTAGAA GAGAATCAAA TGAAGTGGTT
    CACCTAGAAG CACAGAGTGA TCCCCTTGGG GCTACTGCCA ACAGAGCACA AGCATCTGCA
    TCATCAAGTA ACGCTGCTAC AGGTGGCTCC CCATCAGATT CAGCCCACAG TGGAAGAAAT
    ACAGGAATAA CAGGAATCCT TCCTGGTTCC TTATTTCGAT TTGCAGTCCC CCCAGCACTT
    GGAAATAATC TGACCGACAA TGTCATGATT ACTGTAGATA TTATTCCTTC AGGGTGGAGT
    TCATCTGATG GAAAAAGTGA TAAAACTAAA AGTGCACCTT CAAGAGATCC AGAAAGACTG
    CAGAAAATAA AAGAGAGCCT CCTTTTAGAG GATTCTGAAG AAGAAGAAGG TGACTTATGT
    AGGATTTGTC AGATGGCCGC TGCATCGTCA TCTAATTTAC TGATAGAGCC ATGCAAGTGC
    ACAGGAAGTT TGCAGTATGT CCACCAAGAA TGTATGAAAA AGTGGCTACA GGCCAAAATT
    AACTCCGGTT CTTCATTAGA GGCTGTAACC ACCTGTGAAC TCTGTAAAGA GAAGTTGCAG
    CTTAACCTGG AGGATTTTGA TATTCATGAA CTACATAGAG CTCATGCAAA TGAACAAGCT
    GAGTATGAGT TTATCAGCTC TGGTCTCTAC CTAGTTGTGT TATTGCACTT GTGCGAACAA
    AGCTTTTCTG ATATGATGGG AAATACAAAT GAACCAAGCA CACGTGTCCG ATTTATTAAC
    CTTGCAAGAA CTCTTCAGGC ACATATGGAA GATCTCGAAA CTTCAGAGGA TGATTCCGAA
    GAAGATGGAG ACCATAACAG AACATTTGAT ATTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284750
    Clone ID Related Accession (Same CDS sequence) XM_022414868.1
    Accession Version XM_022414868.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MARCH7 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TGGTCAAGAC
    AGAGTTCCTT CTTCATATTC ACAGGGAGCA AGACCAAAAG ATAACTCAGT GAGCACTTTA
    CAGTTGAATA CATCATCCAC AAATCACCAA ATGCCTTCTG AACATCATAC CATACCATGT
    TCTAGGGACA ACAGTAGAAA TTCGCTAAGA TCAAATTTTT CTCCAAGGGA ATTGGAATCT
    CCACAAAGCA GTACACAGCC TGGATTTTCT TATATATCAA ATAGAGATGA AACCTCAACC
    ATAAGCAGTT CAGATAGGGT TGGTTCATCT CAGAGATCAT TTCAAGAATC TTCTGACAAT
    GAAAGTAGAC GTTCGACTAG GAGATTGCTA TCACGTATAG CTTCTAGTAT GTCATCTACC
    TTTTTTTCTC GAAGATCTAG CCAGGATTCC TTGAATACAA GATCACTGAG TTCTGAAAAT
    TCTTACGTGT CTCCAAGAAT CTTGACCGCT TCACAGTCTC GTAGCAATGC AGCATCAACT
    TCCGATGTTC CTGATAGTAG AGCATCTGAA GCTTCTCAGG GATTTCGATT TCTTAGGCGA
    AGATGGGGTT TGTCATCTCT TAGCCAAAAT CATAGCTCTG AGCCAGATTC AGAAAATTTT
    AACCAAGAAT CTGAAGGTAG AAATACAGGA CCGTGGTTAT CTTCCTCACT TAGAAATAGA
    TGCACACCTT TGTTCTCCAG AAGGAGACGA GAGGGACGAG ATGAATCTTC AAGGATACCT
    ACCTCTGATA TACCATCTAG ATCTCATCAT ATTTTTAGAA GAGAATCAAA TGAAGTGGTT
    CACCTAGAAG CACAGAGTGA TCCCCTTGGG GCTACTGCCA ACAGAGCACA AGCATCTGCA
    TCATCAAGTA ACGCTGCTAC AGGTGGCTCC CCATCAGATT CAGCCCACAG TGGAAGAAAT
    ACAGGAATAA CAGGAATCCT TCCTGGTTCC TTATTTCGAT TTGCAGTCCC CCCAGCACTT
    GGAAATAATC TGACCGACAA TGTCATGATT ACTGTAGATA TTATTCCTTC AGGGTGGAGT
    TCATCTGATG GAAAAAGTGA TAAAACTAAA AGTGCACCTT CAAGAGATCC AGAAAGACTG
    CAGAAAATAA AAGAGAGCCT CCTTTTAGAG GATTCTGAAG AAGAAGAAGG TGACTTATGT
    AGGATTTGTC AGATGGCCGC TGCATCGTCA TCTAATTTAC TGATAGAGCC ATGCAAGTGC
    ACAGGAAGTT TGCAGTATGT CCACCAAGAA TGTATGAAAA AGTGGCTACA GGCCAAAATT
    AACTCCGGTT CTTCATTAGA GGCTGTAACC ACCTGTGAAC TCTGTAAAGA GAAGTTGCAG
    CTTAACCTGG AGGATTTTGA TATTCATGAA CTACATAGAG CTCATGCAAA TGAACAAGCT
    GAGTATGAGT TTATCAGCTC TGGTCTCTAC CTAGTTGTGT TATTGCACTT GTGCGAACAA
    AGCTTTTCTG ATATGATGGG AAATACAAAT GAACCAAGCA CACGTGTCCG ACTTCAGAGG
    ATGATTCCGA AGAAGATGGA GACCATAACA GAACATTTGA TATTGCCTAA CTCCATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270576.1
    CDS194..2113
    Translation

    Target ORF information:

    RefSeq Version XM_022414868.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414868.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TGGTCAAGAC
    AGAGTTCCTT CTTCATATTC ACAGGGAGCA AGACCAAAAG ATAACTCAGT GAGCACTTTA
    CAGTTGAATA CATCATCCAC AAATCACCAA ATGCCTTCTG AACATCATAC CATACCATGT
    TCTAGGGACA ACAGTAGAAA TTCGCTAAGA TCAAATTTTT CTCCAAGGGA ATTGGAATCT
    CCACAAAGCA GTACACAGCC TGGATTTTCT TATATATCAA ATAGAGATGA AACCTCAACC
    ATAAGCAGTT CAGATAGGGT TGGTTCATCT CAGAGATCAT TTCAAGAATC TTCTGACAAT
    GAAAGTAGAC GTTCGACTAG GAGATTGCTA TCACGTATAG CTTCTAGTAT GTCATCTACC
    TTTTTTTCTC GAAGATCTAG CCAGGATTCC TTGAATACAA GATCACTGAG TTCTGAAAAT
    TCTTACGTGT CTCCAAGAAT CTTGACCGCT TCACAGTCTC GTAGCAATGC AGCATCAACT
    TCCGATGTTC CTGATAGTAG AGCATCTGAA GCTTCTCAGG GATTTCGATT TCTTAGGCGA
    AGATGGGGTT TGTCATCTCT TAGCCAAAAT CATAGCTCTG AGCCAGATTC AGAAAATTTT
    AACCAAGAAT CTGAAGGTAG AAATACAGGA CCGTGGTTAT CTTCCTCACT TAGAAATAGA
    TGCACACCTT TGTTCTCCAG AAGGAGACGA GAGGGACGAG ATGAATCTTC AAGGATACCT
    ACCTCTGATA TACCATCTAG ATCTCATCAT ATTTTTAGAA GAGAATCAAA TGAAGTGGTT
    CACCTAGAAG CACAGAGTGA TCCCCTTGGG GCTACTGCCA ACAGAGCACA AGCATCTGCA
    TCATCAAGTA ACGCTGCTAC AGGTGGCTCC CCATCAGATT CAGCCCACAG TGGAAGAAAT
    ACAGGAATAA CAGGAATCCT TCCTGGTTCC TTATTTCGAT TTGCAGTCCC CCCAGCACTT
    GGAAATAATC TGACCGACAA TGTCATGATT ACTGTAGATA TTATTCCTTC AGGGTGGAGT
    TCATCTGATG GAAAAAGTGA TAAAACTAAA AGTGCACCTT CAAGAGATCC AGAAAGACTG
    CAGAAAATAA AAGAGAGCCT CCTTTTAGAG GATTCTGAAG AAGAAGAAGG TGACTTATGT
    AGGATTTGTC AGATGGCCGC TGCATCGTCA TCTAATTTAC TGATAGAGCC ATGCAAGTGC
    ACAGGAAGTT TGCAGTATGT CCACCAAGAA TGTATGAAAA AGTGGCTACA GGCCAAAATT
    AACTCCGGTT CTTCATTAGA GGCTGTAACC ACCTGTGAAC TCTGTAAAGA GAAGTTGCAG
    CTTAACCTGG AGGATTTTGA TATTCATGAA CTACATAGAG CTCATGCAAA TGAACAAGCT
    GAGTATGAGT TTATCAGCTC TGGTCTCTAC CTAGTTGTGT TATTGCACTT GTGCGAACAA
    AGCTTTTCTG ATATGATGGG AAATACAAAT GAACCAAGCA CACGTGTCCG ACTTCAGAGG
    ATGATTCCGA AGAAGATGGA GACCATAACA GAACATTTGA TATTGCCTAA CTCCATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284751
    Clone ID Related Accession (Same CDS sequence) XM_022414869.1
    Accession Version XM_022414869.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MARCH7 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGATTCTT CTTGGAGGCA TAATCAAGTT CCTAGATCTT CATCAATGGT ACTTGGATCA 
    TTTGGAACTG ACTTAATGAG AGAGAGAAGA GATTTAGAGA GAAGAGCAGA TTCTTCCATT
    AGTAATCTTA TGGATTATAG TCACCGAAGT GGTGATTTCA CAACTTCATC ATATGGTCAA
    GACAGAGTTC CTTCTTCATA TTCACAGGGA GCAAGACCAA AAGATAACTC AGTGAGCACT
    TTACAGTTGA ATACATCATC CACAAATCAC CAAATGCCTT CTGAACATCA TACCATACCA
    TGTTCTAGGG ACAACAGTAG AAATTCGCTA AGATCAAATT TTTCTCCAAG GGAATTGGAA
    TCTCCACAAA GCAGTACACA GCCTGGATTT TCTTATATAT CAAATAGAGA TGAAACCTCA
    ACCATAAGCA GTTCAGATAG GGTTGGTTCA TCTCAGAGAT CATTTCAAGA ATCTTCTGAC
    AATGAAAGTA GACGTTCGAC TAGGAGATTG CTATCACGTA TAGCTTCTAG TATGTCATCT
    ACCTTTTTTT CTCGAAGATC TAGCCAGGAT TCCTTGAATA CAAGATCACT GAGTTCTGAA
    AATTCTTACG TGTCTCCAAG AATCTTGACC GCTTCACAGT CTCGTAGCAA TGCAGCATCA
    ACTTCCGATG TTCCTGATAG TAGAGCATCT GAAGCTTCTC AGGGATTTCG ATTTCTTAGG
    CGAAGATGGG GTTTGTCATC TCTTAGCCAA AATCATAGCT CTGAGCCAGA TTCAGAAAAT
    TTTAACCAAG AATCTGAAGG TAGAAATACA GGACCGTGGT TATCTTCCTC ACTTAGAAAT
    AGATGCACAC CTTTGTTCTC CAGAAGGAGA CGAGAGGGAC GAGATGAATC TTCAAGGATA
    CCTACCTCTG ATATACCATC TAGATCTCAT CATATTTTTA GAAGAGAATC AAATGAAGTG
    GTTCACCTAG AAGCACAGAG TGATCCCCTT GGGGCTACTG CCAACAGAGC ACAAGCATCT
    GCATCATCAA GTAACGCTGC TACAGGTGGC TCCCCATCAG ATTCAGCCCA CAGTGGAAGA
    AATACAGGAA TAACAGGAAT CCTTCCTGGT TCCTTATTTC GATTTGCAGT CCCCCCAGCA
    CTTGGAAATA ATCTGACCGA CAATGTCATG ATTACTGTAG ATATTATTCC TTCAGGGTGG
    AGTTCATCTG ATGGAAAAAG TGATAAAACT AAAAGTGCAC CTTCAAGAGA TCCAGAAAGA
    CTGCAGAAAA TAAAAGAGAG CCTCCTTTTA GAGGATTCTG AAGAAGAAGA AGGTGACTTA
    TGTAGGATTT GTCAGATGGC CGCTGCATCG TCATCTAATT TACTGATAGA GCCATGCAAG
    TGCACAGGAA GTTTGCAGTA TGTCCACCAA GAATGTATGA AAAAGTGGCT ACAGGCCAAA
    ATTAACTCCG GTTCTTCATT AGAGGCTGTA ACCACCTGTG AACTCTGTAA AGAGAAGTTG
    CAGCTTAACC TGGAGGATTT TGATATTCAT GAACTACATA GAGCTCATGC AAATGAACAA
    GCTGAGTATG AGTTTATCAG CTCTGGTCTC TACCTAGTTG TGTTATTGCA CTTGTGCGAA
    CAAAGCTTTT CTGATATGAT GGGAAATACA AATGAACCAA GCACACGTGT CCGATTTATT
    AACCTTGCAA GAACTCTTCA GGCACATATG GAAGATCTCG AAACTTCAGA GGATGATTCC
    GAAGAAGATG GAGACCATAA CAGAACATTT GATATTGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270577.1
    CDS195..1976
    Translation

    Target ORF information:

    RefSeq Version XM_022414869.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414869.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGATTCTT CTTGGAGGCA TAATCAAGTT CCTAGATCTT CATCAATGGT ACTTGGATCA 
    TTTGGAACTG ACTTAATGAG AGAGAGAAGA GATTTAGAGA GAAGAGCAGA TTCTTCCATT
    AGTAATCTTA TGGATTATAG TCACCGAAGT GGTGATTTCA CAACTTCATC ATATGGTCAA
    GACAGAGTTC CTTCTTCATA TTCACAGGGA GCAAGACCAA AAGATAACTC AGTGAGCACT
    TTACAGTTGA ATACATCATC CACAAATCAC CAAATGCCTT CTGAACATCA TACCATACCA
    TGTTCTAGGG ACAACAGTAG AAATTCGCTA AGATCAAATT TTTCTCCAAG GGAATTGGAA
    TCTCCACAAA GCAGTACACA GCCTGGATTT TCTTATATAT CAAATAGAGA TGAAACCTCA
    ACCATAAGCA GTTCAGATAG GGTTGGTTCA TCTCAGAGAT CATTTCAAGA ATCTTCTGAC
    AATGAAAGTA GACGTTCGAC TAGGAGATTG CTATCACGTA TAGCTTCTAG TATGTCATCT
    ACCTTTTTTT CTCGAAGATC TAGCCAGGAT TCCTTGAATA CAAGATCACT GAGTTCTGAA
    AATTCTTACG TGTCTCCAAG AATCTTGACC GCTTCACAGT CTCGTAGCAA TGCAGCATCA
    ACTTCCGATG TTCCTGATAG TAGAGCATCT GAAGCTTCTC AGGGATTTCG ATTTCTTAGG
    CGAAGATGGG GTTTGTCATC TCTTAGCCAA AATCATAGCT CTGAGCCAGA TTCAGAAAAT
    TTTAACCAAG AATCTGAAGG TAGAAATACA GGACCGTGGT TATCTTCCTC ACTTAGAAAT
    AGATGCACAC CTTTGTTCTC CAGAAGGAGA CGAGAGGGAC GAGATGAATC TTCAAGGATA
    CCTACCTCTG ATATACCATC TAGATCTCAT CATATTTTTA GAAGAGAATC AAATGAAGTG
    GTTCACCTAG AAGCACAGAG TGATCCCCTT GGGGCTACTG CCAACAGAGC ACAAGCATCT
    GCATCATCAA GTAACGCTGC TACAGGTGGC TCCCCATCAG ATTCAGCCCA CAGTGGAAGA
    AATACAGGAA TAACAGGAAT CCTTCCTGGT TCCTTATTTC GATTTGCAGT CCCCCCAGCA
    CTTGGAAATA ATCTGACCGA CAATGTCATG ATTACTGTAG ATATTATTCC TTCAGGGTGG
    AGTTCATCTG ATGGAAAAAG TGATAAAACT AAAAGTGCAC CTTCAAGAGA TCCAGAAAGA
    CTGCAGAAAA TAAAAGAGAG CCTCCTTTTA GAGGATTCTG AAGAAGAAGA AGGTGACTTA
    TGTAGGATTT GTCAGATGGC CGCTGCATCG TCATCTAATT TACTGATAGA GCCATGCAAG
    TGCACAGGAA GTTTGCAGTA TGTCCACCAA GAATGTATGA AAAAGTGGCT ACAGGCCAAA
    ATTAACTCCG GTTCTTCATT AGAGGCTGTA ACCACCTGTG AACTCTGTAA AGAGAAGTTG
    CAGCTTAACC TGGAGGATTT TGATATTCAT GAACTACATA GAGCTCATGC AAATGAACAA
    GCTGAGTATG AGTTTATCAG CTCTGGTCTC TACCTAGTTG TGTTATTGCA CTTGTGCGAA
    CAAAGCTTTT CTGATATGAT GGGAAATACA AATGAACCAA GCACACGTGT CCGATTTATT
    AACCTTGCAA GAACTCTTCA GGCACATATG GAAGATCTCG AAACTTCAGA GGATGATTCC
    GAAGAAGATG GAGACCATAA CAGAACATTT GATATTGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284752
    Clone ID Related Accession (Same CDS sequence) XM_022414870.1
    Accession Version XM_022414870.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MARCH7 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGTACTTG GATCATTTGG AACTGACTTA ATGAGAGAGA GAAGAGATTT AGAGAGAAGA 
    GCAGATTCTT CCATTAGTAA TCTTATGGAT TATAGTCACC GAAGTGGTGA TTTCACAACT
    TCATCATATG GTCAAGACAG AGTTCCTTCT TCATATTCAC AGGGAGCAAG ACCAAAAGAT
    AACTCAGTGA GCACTTTACA GTTGAATACA TCATCCACAA ATCACCAAAT GCCTTCTGAA
    CATCATACCA TACCATGTTC TAGGGACAAC AGTAGAAATT CGCTAAGATC AAATTTTTCT
    CCAAGGGAAT TGGAATCTCC ACAAAGCAGT ACACAGCCTG GATTTTCTTA TATATCAAAT
    AGAGATGAAA CCTCAACCAT AAGCAGTTCA GATAGGGTTG GTTCATCTCA GAGATCATTT
    CAAGAATCTT CTGACAATGA AAGTAGACGT TCGACTAGGA GATTGCTATC ACGTATAGCT
    TCTAGTATGT CATCTACCTT TTTTTCTCGA AGATCTAGCC AGGATTCCTT GAATACAAGA
    TCACTGAGTT CTGAAAATTC TTACGTGTCT CCAAGAATCT TGACCGCTTC ACAGTCTCGT
    AGCAATGCAG CATCAACTTC CGATGTTCCT GATAGTAGAG CATCTGAAGC TTCTCAGGGA
    TTTCGATTTC TTAGGCGAAG ATGGGGTTTG TCATCTCTTA GCCAAAATCA TAGCTCTGAG
    CCAGATTCAG AAAATTTTAA CCAAGAATCT GAAGGTAGAA ATACAGGACC GTGGTTATCT
    TCCTCACTTA GAAATAGATG CACACCTTTG TTCTCCAGAA GGAGACGAGA GGGACGAGAT
    GAATCTTCAA GGATACCTAC CTCTGATATA CCATCTAGAT CTCATCATAT TTTTAGAAGA
    GAATCAAATG AAGTGGTTCA CCTAGAAGCA CAGAGTGATC CCCTTGGGGC TACTGCCAAC
    AGAGCACAAG CATCTGCATC ATCAAGTAAC GCTGCTACAG GTGGCTCCCC ATCAGATTCA
    GCCCACAGTG GAAGAAATAC AGGAATAACA GGAATCCTTC CTGGTTCCTT ATTTCGATTT
    GCAGTCCCCC CAGCACTTGG AAATAATCTG ACCGACAATG TCATGATTAC TGTAGATATT
    ATTCCTTCAG GGTGGAGTTC ATCTGATGGA AAAAGTGATA AAACTAAAAG TGCACCTTCA
    AGAGATCCAG AAAGACTGCA GAAAATAAAA GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA
    GAAGAAGGTG ACTTATGTAG GATTTGTCAG ATGGCCGCTG CATCGTCATC TAATTTACTG
    ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG CAGTATGTCC ACCAAGAATG TATGAAAAAG
    TGGCTACAGG CCAAAATTAA CTCCGGTTCT TCATTAGAGG CTGTAACCAC CTGTGAACTC
    TGTAAAGAGA AGTTGCAGCT TAACCTGGAG GATTTTGATA TTCATGAACT ACATAGAGCT
    CATGCAAATG AACAAGCTGA GTATGAGTTT ATCAGCTCTG GTCTCTACCT AGTTGTGTTA
    TTGCACTTGT GCGAACAAAG CTTTTCTGAT ATGATGGGAA ATACAAATGA ACCAAGCACA
    CGTGTCCGAT TTATTAACCT TGCAAGAACT CTTCAGGCAC ATATGGAAGA TCTCGAAACT
    TCAGAGGATG ATTCCGAAGA AGATGGAGAC CATAACAGAA CATTTGATAT TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270578.1
    CDS195..1931
    Translation

    Target ORF information:

    RefSeq Version XM_022414870.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414870.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGTACTTG GATCATTTGG AACTGACTTA ATGAGAGAGA GAAGAGATTT AGAGAGAAGA 
    GCAGATTCTT CCATTAGTAA TCTTATGGAT TATAGTCACC GAAGTGGTGA TTTCACAACT
    TCATCATATG GTCAAGACAG AGTTCCTTCT TCATATTCAC AGGGAGCAAG ACCAAAAGAT
    AACTCAGTGA GCACTTTACA GTTGAATACA TCATCCACAA ATCACCAAAT GCCTTCTGAA
    CATCATACCA TACCATGTTC TAGGGACAAC AGTAGAAATT CGCTAAGATC AAATTTTTCT
    CCAAGGGAAT TGGAATCTCC ACAAAGCAGT ACACAGCCTG GATTTTCTTA TATATCAAAT
    AGAGATGAAA CCTCAACCAT AAGCAGTTCA GATAGGGTTG GTTCATCTCA GAGATCATTT
    CAAGAATCTT CTGACAATGA AAGTAGACGT TCGACTAGGA GATTGCTATC ACGTATAGCT
    TCTAGTATGT CATCTACCTT TTTTTCTCGA AGATCTAGCC AGGATTCCTT GAATACAAGA
    TCACTGAGTT CTGAAAATTC TTACGTGTCT CCAAGAATCT TGACCGCTTC ACAGTCTCGT
    AGCAATGCAG CATCAACTTC CGATGTTCCT GATAGTAGAG CATCTGAAGC TTCTCAGGGA
    TTTCGATTTC TTAGGCGAAG ATGGGGTTTG TCATCTCTTA GCCAAAATCA TAGCTCTGAG
    CCAGATTCAG AAAATTTTAA CCAAGAATCT GAAGGTAGAA ATACAGGACC GTGGTTATCT
    TCCTCACTTA GAAATAGATG CACACCTTTG TTCTCCAGAA GGAGACGAGA GGGACGAGAT
    GAATCTTCAA GGATACCTAC CTCTGATATA CCATCTAGAT CTCATCATAT TTTTAGAAGA
    GAATCAAATG AAGTGGTTCA CCTAGAAGCA CAGAGTGATC CCCTTGGGGC TACTGCCAAC
    AGAGCACAAG CATCTGCATC ATCAAGTAAC GCTGCTACAG GTGGCTCCCC ATCAGATTCA
    GCCCACAGTG GAAGAAATAC AGGAATAACA GGAATCCTTC CTGGTTCCTT ATTTCGATTT
    GCAGTCCCCC CAGCACTTGG AAATAATCTG ACCGACAATG TCATGATTAC TGTAGATATT
    ATTCCTTCAG GGTGGAGTTC ATCTGATGGA AAAAGTGATA AAACTAAAAG TGCACCTTCA
    AGAGATCCAG AAAGACTGCA GAAAATAAAA GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA
    GAAGAAGGTG ACTTATGTAG GATTTGTCAG ATGGCCGCTG CATCGTCATC TAATTTACTG
    ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG CAGTATGTCC ACCAAGAATG TATGAAAAAG
    TGGCTACAGG CCAAAATTAA CTCCGGTTCT TCATTAGAGG CTGTAACCAC CTGTGAACTC
    TGTAAAGAGA AGTTGCAGCT TAACCTGGAG GATTTTGATA TTCATGAACT ACATAGAGCT
    CATGCAAATG AACAAGCTGA GTATGAGTTT ATCAGCTCTG GTCTCTACCT AGTTGTGTTA
    TTGCACTTGT GCGAACAAAG CTTTTCTGAT ATGATGGGAA ATACAAATGA ACCAAGCACA
    CGTGTCCGAT TTATTAACCT TGCAAGAACT CTTCAGGCAC ATATGGAAGA TCTCGAAACT
    TCAGAGGATG ATTCCGAAGA AGATGGAGAC CATAACAGAA CATTTGATAT TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31002
    Clone ID Related Accession (Same CDS sequence) XM_005640221.2 , XM_005640222.2
    Accession Version XM_005640221.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MARCH7 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726122.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640221.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAT TTATTAACCT TGCAAGAACT
    CTTCAGGCAC ATATGGAAGA TCTCGAAACG GTAATAAACC AGATTCTACT CAGGTGTGTG
    AGAGCACTTG CCTTAAGTTC ATTTTTTCTC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640278.1
    CDS188..2320
    Translation

    Target ORF information:

    RefSeq Version XM_005640221.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase (MARCH7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640221.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAT TTATTAACCT TGCAAGAACT
    CTTCAGGCAC ATATGGAAGA TCTCGAAACG GTAATAAACC AGATTCTACT CAGGTGTGTG
    AGAGCACTTG CCTTAAGTTC ATTTTTTCTC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31002
    Clone ID Related Accession (Same CDS sequence) XM_005640221.2 , XM_005640222.2
    Accession Version XM_005640222.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MARCH7 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726122.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640222.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAT TTATTAACCT TGCAAGAACT
    CTTCAGGCAC ATATGGAAGA TCTCGAAACG GTAATAAACC AGATTCTACT CAGGTGTGTG
    AGAGCACTTG CCTTAAGTTC ATTTTTTCTC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640279.1
    CDS319..2451
    Translation

    Target ORF information:

    RefSeq Version XM_005640222.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase (MARCH7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640222.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAT TTATTAACCT TGCAAGAACT
    CTTCAGGCAC ATATGGAAGA TCTCGAAACG GTAATAAACC AGATTCTACT CAGGTGTGTG
    AGAGCACTTG CCTTAAGTTC ATTTTTTCTC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31003
    Clone ID Related Accession (Same CDS sequence) XM_022414865.1 , XM_843631.4 , XM_843631.5
    Accession Version XM_022414865.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MARCH7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAT TTATTAACCT TGCAAGAACT
    CTTCAGGCAC ATATGGAAGA TCTCGAAACT TCAGAGGATG ATTCCGAAGA AGATGGAGAC
    CATAACAGAA CATTTGATAT TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270573.1
    CDS319..2445
    Translation

    Target ORF information:

    RefSeq Version XM_022414865.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414865.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAT TTATTAACCT TGCAAGAACT
    CTTCAGGCAC ATATGGAAGA TCTCGAAACT TCAGAGGATG ATTCCGAAGA AGATGGAGAC
    CATAACAGAA CATTTGATAT TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31003
    Clone ID Related Accession (Same CDS sequence) XM_022414865.1 , XM_843631.4 , XM_843631.5
    Accession Version XM_843631.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MARCH7 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726122.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_843631.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAT TTATTAACCT TGCAAGAACT
    CTTCAGGCAC ATATGGAAGA TCTCGAAACT TCAGAGGATG ATTCCGAAGA AGATGGAGAC
    CATAACAGAA CATTTGATAT TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_848724.1
    CDS187..2313
    Translation

    Target ORF information:

    RefSeq Version XM_843631.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase (MARCH7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_843631.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAT TTATTAACCT TGCAAGAACT
    CTTCAGGCAC ATATGGAAGA TCTCGAAACT TCAGAGGATG ATTCCGAAGA AGATGGAGAC
    CATAACAGAA CATTTGATAT TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31003
    Clone ID Related Accession (Same CDS sequence) XM_022414865.1 , XM_843631.4 , XM_843631.5
    Accession Version XM_843631.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MARCH7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_843631.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAT TTATTAACCT TGCAAGAACT
    CTTCAGGCAC ATATGGAAGA TCTCGAAACT TCAGAGGATG ATTCCGAAGA AGATGGAGAC
    CATAACAGAA CATTTGATAT TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_848724.1
    CDS192..2318
    Translation

    Target ORF information:

    RefSeq Version XM_843631.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_843631.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAT TTATTAACCT TGCAAGAACT
    CTTCAGGCAC ATATGGAAGA TCTCGAAACT TCAGAGGATG ATTCCGAAGA AGATGGAGAC
    CATAACAGAA CATTTGATAT TGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31004
    Clone ID Related Accession (Same CDS sequence) XM_005640224.2 , XM_005640224.3
    Accession Version XM_005640224.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2106bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MARCH7 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726122.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640224.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAT TTATTAACCT TGCAAGAACT
    CTTCAGGCAC ATATGGAAGA TCTCGAAACG GTAATAAACC AGATTCTACT CAGCTTCAGA
    GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640281.1
    CDS187..2292
    Translation

    Target ORF information:

    RefSeq Version XM_005640224.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase (MARCH7), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640224.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAT TTATTAACCT TGCAAGAACT
    CTTCAGGCAC ATATGGAAGA TCTCGAAACG GTAATAAACC AGATTCTACT CAGCTTCAGA
    GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31004
    Clone ID Related Accession (Same CDS sequence) XM_005640224.2 , XM_005640224.3
    Accession Version XM_005640224.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2106bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MARCH7 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640224.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAT TTATTAACCT TGCAAGAACT
    CTTCAGGCAC ATATGGAAGA TCTCGAAACG GTAATAAACC AGATTCTACT CAGCTTCAGA
    GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640281.1
    CDS192..2297
    Translation

    Target ORF information:

    RefSeq Version XM_005640224.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640224.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAT TTATTAACCT TGCAAGAACT
    CTTCAGGCAC ATATGGAAGA TCTCGAAACG GTAATAAACC AGATTCTACT CAGCTTCAGA
    GGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31005
    Clone ID Related Accession (Same CDS sequence) XM_005640225.3 , XM_005640225.2
    Accession Version XM_005640225.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MARCH7 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640225.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAC TTCAGAGGAT GATTCCGAAG
    AAGATGGAGA CCATAACAGA ACATTTGATA TTGCCTAACT CCATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640282.1
    CDS192..2279
    Translation

    Target ORF information:

    RefSeq Version XM_005640225.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris membrane associated ring-CH-type finger 7 (MARCH7), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640225.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAC TTCAGAGGAT GATTCCGAAG
    AAGATGGAGA CCATAACAGA ACATTTGATA TTGCCTAACT CCATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31005
    Clone ID Related Accession (Same CDS sequence) XM_005640225.3 , XM_005640225.2
    Accession Version XM_005640225.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MARCH7 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726122.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640225.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAC TTCAGAGGAT GATTCCGAAG
    AAGATGGAGA CCATAACAGA ACATTTGATA TTGCCTAACT CCATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640282.1
    CDS187..2274
    Translation

    Target ORF information:

    RefSeq Version XM_005640225.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase (MARCH7), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640225.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAGTCTA AACCTTCAAG GATTCCAAGA AGAATTTCTG TTCAACCTTC TAGCTCTGTA 
    AGTGCTAGAA TGATGTCTGG AAGCAGAGGA AATAGTTTAA ATGATACCTA TCACTCAAGA
    GATTCTTCAT TTAGACTGGA TTCTGAATAT CAGTCCACAT CAGCATCAGC ATCTGCATCA
    CCATTTCAAT CTACATGGTA TAGTGAATCT GAGATAACTC AGGGAGCACG CTCAAGATCA
    CAAAACCAAC AACGGGATCA TGATTCAAAA AGACCTAAAC TTTCCTGTAC AAATTGTACA
    TCTACCTCAG TCGGGAGAAA TATTGGACAT GGTTTAAATG CAGTACCAGA TTCTTCTTGG
    AGGCATAATC AAGTTCCTAG ATCTTCATCA ATGGTACTTG GATCATTTGG AACTGACTTA
    ATGAGAGAGA GAAGAGATTT AGAGAGAAGA GCAGATTCTT CCATTAGTAA TCTTATGGAT
    TATAGTCACC GAAGTGGTGA TTTCACAACT TCATCATATG GTCAAGACAG AGTTCCTTCT
    TCATATTCAC AGGGAGCAAG ACCAAAAGAT AACTCAGTGA GCACTTTACA GTTGAATACA
    TCATCCACAA ATCACCAAAT GCCTTCTGAA CATCATACCA TACCATGTTC TAGGGACAAC
    AGTAGAAATT CGCTAAGATC AAATTTTTCT CCAAGGGAAT TGGAATCTCC ACAAAGCAGT
    ACACAGCCTG GATTTTCTTA TATATCAAAT AGAGATGAAA CCTCAACCAT AAGCAGTTCA
    GATAGGGTTG GTTCATCTCA GAGATCATTT CAAGAATCTT CTGACAATGA AAGTAGACGT
    TCGACTAGGA GATTGCTATC ACGTATAGCT TCTAGTATGT CATCTACCTT TTTTTCTCGA
    AGATCTAGCC AGGATTCCTT GAATACAAGA TCACTGAGTT CTGAAAATTC TTACGTGTCT
    CCAAGAATCT TGACCGCTTC ACAGTCTCGT AGCAATGCAG CATCAACTTC CGATGTTCCT
    GATAGTAGAG CATCTGAAGC TTCTCAGGGA TTTCGATTTC TTAGGCGAAG ATGGGGTTTG
    TCATCTCTTA GCCAAAATCA TAGCTCTGAG CCAGATTCAG AAAATTTTAA CCAAGAATCT
    GAAGGTAGAA ATACAGGACC GTGGTTATCT TCCTCACTTA GAAATAGATG CACACCTTTG
    TTCTCCAGAA GGAGACGAGA GGGACGAGAT GAATCTTCAA GGATACCTAC CTCTGATATA
    CCATCTAGAT CTCATCATAT TTTTAGAAGA GAATCAAATG AAGTGGTTCA CCTAGAAGCA
    CAGAGTGATC CCCTTGGGGC TACTGCCAAC AGAGCACAAG CATCTGCATC ATCAAGTAAC
    GCTGCTACAG GTGGCTCCCC ATCAGATTCA GCCCACAGTG GAAGAAATAC AGGAATAACA
    GGAATCCTTC CTGGTTCCTT ATTTCGATTT GCAGTCCCCC CAGCACTTGG AAATAATCTG
    ACCGACAATG TCATGATTAC TGTAGATATT ATTCCTTCAG GGTGGAGTTC ATCTGATGGA
    AAAAGTGATA AAACTAAAAG TGCACCTTCA AGAGATCCAG AAAGACTGCA GAAAATAAAA
    GAGAGCCTCC TTTTAGAGGA TTCTGAAGAA GAAGAAGGTG ACTTATGTAG GATTTGTCAG
    ATGGCCGCTG CATCGTCATC TAATTTACTG ATAGAGCCAT GCAAGTGCAC AGGAAGTTTG
    CAGTATGTCC ACCAAGAATG TATGAAAAAG TGGCTACAGG CCAAAATTAA CTCCGGTTCT
    TCATTAGAGG CTGTAACCAC CTGTGAACTC TGTAAAGAGA AGTTGCAGCT TAACCTGGAG
    GATTTTGATA TTCATGAACT ACATAGAGCT CATGCAAATG AACAAGCTGA GTATGAGTTT
    ATCAGCTCTG GTCTCTACCT AGTTGTGTTA TTGCACTTGT GCGAACAAAG CTTTTCTGAT
    ATGATGGGAA ATACAAATGA ACCAAGCACA CGTGTCCGAC TTCAGAGGAT GATTCCGAAG
    AAGATGGAGA CCATAACAGA ACATTTGATA TTGCCTAACT CCATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.