EIF2AK4 cDNA ORF clone, Canis lupus familiaris(dog)

The following EIF2AK4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF2AK4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe28640 XM_005638232.2
Latest version!
Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe28640 XM_022412709.1
Latest version!
Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe28636 XM_535431.6
Latest version!
Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe28637 XM_005638229.3
Latest version!
Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe28638 XM_005638230.2
Latest version!
Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe28639 XM_005638231.3
Latest version!
Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OCe28639 XM_005638231.2 Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe28637 XM_005638229.2 Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe28636 XM_535431.5 Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe28640
    Clone ID Related Accession (Same CDS sequence) XM_005638232.2 , XM_022412709.1
    Accession Version XM_005638232.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product eukaryotic translation initiation factor 2-alpha kinase 4 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638232.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTAAACA TCGGACCAAC TCCTCTGGAA GGTCTAGGCG AGAACGTCAG TATTCTCTGT 
    GTAGTAGTGA AGATTCTTCT GGCTCTTGTG AAGTCCTGTA TTTCTCTATG GGTAGCCCTG
    ATCAGCTCAT GGTTTACAAA GGGAGGTGCA TTGATTCAAG GGGCAGAAAC GGAATTCAAC
    TCACTGGTAA AATTGAGCCA TCCAAATGTA GTACGCTACT TTGCAATGAA TCTCAAAGAG
    CAAGATGACT CCATCATGGT GGACATTTTA TTGGAGCACA TTAATGGGGT CTCCCTCTCT
    GCACATCTGA GCCACTCAGG CCCCATCCCT GTGTATCAAC TCCGCAAGTA CACAGCTCAG
    CTCTTGTCTG GCCTCGATTA TTTGCACAGC AATGACGTGG TGCACAAGGT TCTGAATGCA
    TCAAATGTCT TGGTGGATGC AGAGGGCAAT GTCAAGATTA CGGACTACAG CATTTCAAAG
    CGCCTAGCAG ACATTTGCAA GGAGGATGTG TTTGAGCAGA GCCGAGTTAG TTTTAGTGAC
    AGTGCCCTAC CTTATAAAAC GGGGAAGAAA GGGGATGTTT GGTGTCTTGG CCTTCTGCTG
    CTCTTCCTCA GCCAAGGACA AGAATGCGGA GAGTACCCTG TGACCATCCC TAGCGACTTA
    CCAGCTGACT TCCAAGATTT TCTAAAGAAG TGTGTTTGCT TGGATGACAA GGAAAGATGG
    AGTCCCCAGC AATTGTTGAA ACACAGCTTT ATCAATCCAC AGCCGAAAAT GTCTTTATTG
    GAACAGAGTC CTGACGATTC TGGAGGACAA GATTATGTTG AGACTGTTAT TCCTAGTAAG
    CAGCTACCCA GTGCAGCATT CTTCAGTGAG ACACAGAGAC AGTTTTCCCG GTATTTCATT
    GAGTTTGAAG AATTACAGCT TCTCGGCAAA GGAGCCTTCG GAGCTGTCAT CAAGGTGCAG
    AACAAGCTGG ATGGCTGCTG CTACGCGGTG AAGCGCATCC CCATCAATCC GGCCAGTAGG
    CATTTCCGCA GGATCAAGGG CGAGGTGACG CTGCTGTCGC GCCTGCACCA CGAGAACATA
    GTGCGCTACT ATAACGCCTG GATAGAGAGA TACGAGCGGC CCGTGGGCCC GGGGATGCTG
    CCGCAGGACT CGGACTCATG GCCCCAGGCA CCGGATGGGC GAGCTTTGTC CCAGCCGTCC
    CCAGGCAACG ACAGCCCCGA CGGCGACGGC GCCGGTAGCG TGGAGGCCGC TGCGCCACCG
    CCCATCCTCA GCTCGGTGGA GTGGAGCACA TCTGGCGAGC GTTCGGCCAG CGCCCGCTTC
    CCCTCCGCCC GCCCAGGCTC CAGCAGCGAG GAGGACGACG ACGACGACGA GCACGAGGGC
    GTCTTCTCCC AGCCCTTCCT ACCTGCTTCA GATTCTGACA GTGATGTCAT CTTTGAAGAT
    GAGAACAGTA AGAGTCAGAA TCAGGAAGAA GACTGCAATG GGAAGAACAG TTGCCATGAA
    AGTGAGCCAT CGACAACCAC TGAAGCTGTG CACTACCTGT ATATCCAGAT GGAGTACTGT
    GAGAAGAGTA CTTTGCGTGA CACCATTGAC CAGGGGCTAT ATCAAGATAC CATCAGACTC
    TGGAGGCTTT TTCGAGAGAT TCTGGATGGA TTAGCTTATA TCCATGAAAA AGGAATGATT
    CACCGGGATT TGAAGCCTGT CAACATCTTT TTGGATTCTG ATGACCATGT AAAAATAGGT
    GATTTTGGTT TAGCGACAGA CCATCTTGCC TTCACTGCTG ATGGCAAGCA AGATGATACA
    GCAGATCACT TGATTAAATC AGACCCCTCA GGTCATTTGA CTGGGATGGT TGGTACTGCT
    TTATACGTAA GCCCAGAGGT CCAAGGAAGC AGCAGACCCA CGTACAACCA GAAAGTGGAT
    CTCTTCAGCC TGGGAATTAT CTTCTTTGAG ATGTCCTATC ACCCCATGGT CACGGCCTCA
    GAAAGGATCT TTGTTCTCAA CCAACTCAGA GATCCCACTT CACCTAAGTT TCCAGAAGAC
    TTCGATGATG GAGAGCATAC AAAGCAGAAA TCTGTCATCT CCTGGCTGTT GAACCATGAT
    CCCGCCAAGC GGCCCACAGC CACAGAGCTG CTCAAGAGTG AGCTGCTGCC CCCACCCCAG
    ATGGAGGAGT CAGAGTTGCA TGAGGTTCTG CACCACACCC TGGCCAACGT GGATGGGAAG
    GCCTACCGCA CCATGATGGC CCAGATCTTC TCCCAGCGCG TCAGTCCAGC CATCGATTAC
    ACCTATGACA GCAACATGCC GAAGGGCTTT TCAATCCGTA CATCCAAGAT ACAGCAACAT
    GTGTGTGATA CCATCGTCCG CATTTTTAAA AAACACGGAG CTGTCCAGTT GTGTACTCCA
    CTACTGCTTC CCCAAAATAG GCAAATATAC GAGCACAATG AAGCTGCCTT ATTCATGGAC
    CACAGCGGGA TGCTGGTGAT GCTTCCCTTT GACCTACGGG TTCCTTTTGC AAGATATGTG
    GCAAGAAATA ATATATTGAA TTTGAAACGT TACTGCATAG AACGTGTCTT CAGACCTCGC
    AAGTTAGACC GATTTCATCC CAAAGAACTT CTGGAATGTG CATTTGACAT TGTTACGTCT
    ACCACCAACA GCTCTCTGCC CACTGCTGAA GTCATCTACA CGATCTGTGA GATCATCCAG
    GAGTTCCCGG CACTGCAGGA AAGAAATTAC AGTATTTACT TGAACCACAC CATGTTATTG
    AAAGCAATCT TCTTACACTG TGGGATCCCA GAAGATAAAC TCAGTCAAGT CTATGTTATT
    CTATATGATG CAGTGACAGA AAAGCTGACA AGGAGAGAAG TGGAAGCCAA GTTTTGTAAT
    CTGTCTCTGT CATCTAATAG TCTGTGTCGA CTCTACAAAT TCATTGAGCA GAAGGGAGAT
    TTGCAAGATC TAACACCAGC AATAAATTCA TTAATAAAAC AGAAAACAGG TGTTGCCCAA
    CTGGTGAAGT ATGGCTTGAA AGACTTAGAG GAGATCATTG GGCTGTTGAA GAAACTCGGC
    ATCAAGTTAC AGGTCTTGAT CAACTTGGGC TTGGTTTACA AAGTACAGCA GCACAACGGA
    GTCATCTTCC AGTTTGTGGC GGTCATCAGA CGGAGGCAAA GGGCTATTTC AGAAATCCTT
    GCAGCTGGAG GCAGATACGA CCTGCTGATT CCCCAGTTTA GAGGACCGCA GGCTCTGGGA
    CCAGTCCCGA CTGCTGTTGG GGTCAGCATA GCCATAGACA AGATATCTGC AGCTATCCTC
    AACATGGAAG AACCTGTTAC AATAAGCTCT TGTGACCTCC TGATCGTAAG TGTTGGCCAG
    ATGTCCATGT CCAGGGCCAT CAACCTAACC CAGAAACTTT GGTCAGCAGG AATCACAGCA
    CAGATCATGT ACGACTGGTC ACAGTCCCAA GAAGAACTCC AGGAATACTG CAGATACCAC
    GAAATCACCT ACGTGGCCCT CGTCTCAGAT AAAGGAGGAA GCTATGTCAA GGTTAAGTCT
    TTTGAGAAGG AAAGGCAAAC AGAGAAACTT GTGCAGGAGT CTGATCTTAT AGACCACGTT
    TTGCAGAAAC TCAGAACTAA AGTCAGTGAT GAAAGGACTA TCAGAGAAGC TTCTGATAAT
    CTCGCAGTGC AAAATCTGAA GGGGTCCTTT TCTAATGCTT CAGGTTTGTT TGAAATCCAC
    GGAGCAGCAG TTGTCCCCAT TGTGAGCGTG ATAGCCCCAG AGAAGCTGTC AGCCAGCACG
    AGGAGGCGTT ATGAAACTCA GGTACAAACA CGACTTCAGA CCTCCCTTGC CAACTTACAT
    CAGAAAAGCA ATGAAATTGA AATTTTGGCT GTGGATCTAC CCAAAGAAAC AATCTTACAG
    TTTTTATCGT TAGAGTGGGA TGCTGATGAA CAGGCATTTA ACACAACTGT GAAGCAGCTG
    CTGTCACGCC TGCCAAAGCA GAGATACCTC AAATTAGTCT GTGATGAAAT TTATAACATC
    AAAGTAGAGA AAAAGGTATC TGTGCTATTC CTGTATAGCT ACAGAGATGA CTACTACCGA
    ATCTTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638289.1
    CDS97..4188
    Translation

    Target ORF information:

    RefSeq Version XM_005638232.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638232.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTAAACA TCGGACCAAC TCCTCTGGAA GGTCTAGGCG AGAACGTCAG TATTCTCTGT 
    GTAGTAGTGA AGATTCTTCT GGCTCTTGTG AAGTCCTGTA TTTCTCTATG GGTAGCCCTG
    ATCAGCTCAT GGTTTACAAA GGGAGGTGCA TTGATTCAAG GGGCAGAAAC GGAATTCAAC
    TCACTGGTAA AATTGAGCCA TCCAAATGTA GTACGCTACT TTGCAATGAA TCTCAAAGAG
    CAAGATGACT CCATCATGGT GGACATTTTA TTGGAGCACA TTAATGGGGT CTCCCTCTCT
    GCACATCTGA GCCACTCAGG CCCCATCCCT GTGTATCAAC TCCGCAAGTA CACAGCTCAG
    CTCTTGTCTG GCCTCGATTA TTTGCACAGC AATGACGTGG TGCACAAGGT TCTGAATGCA
    TCAAATGTCT TGGTGGATGC AGAGGGCAAT GTCAAGATTA CGGACTACAG CATTTCAAAG
    CGCCTAGCAG ACATTTGCAA GGAGGATGTG TTTGAGCAGA GCCGAGTTAG TTTTAGTGAC
    AGTGCCCTAC CTTATAAAAC GGGGAAGAAA GGGGATGTTT GGTGTCTTGG CCTTCTGCTG
    CTCTTCCTCA GCCAAGGACA AGAATGCGGA GAGTACCCTG TGACCATCCC TAGCGACTTA
    CCAGCTGACT TCCAAGATTT TCTAAAGAAG TGTGTTTGCT TGGATGACAA GGAAAGATGG
    AGTCCCCAGC AATTGTTGAA ACACAGCTTT ATCAATCCAC AGCCGAAAAT GTCTTTATTG
    GAACAGAGTC CTGACGATTC TGGAGGACAA GATTATGTTG AGACTGTTAT TCCTAGTAAG
    CAGCTACCCA GTGCAGCATT CTTCAGTGAG ACACAGAGAC AGTTTTCCCG GTATTTCATT
    GAGTTTGAAG AATTACAGCT TCTCGGCAAA GGAGCCTTCG GAGCTGTCAT CAAGGTGCAG
    AACAAGCTGG ATGGCTGCTG CTACGCGGTG AAGCGCATCC CCATCAATCC GGCCAGTAGG
    CATTTCCGCA GGATCAAGGG CGAGGTGACG CTGCTGTCGC GCCTGCACCA CGAGAACATA
    GTGCGCTACT ATAACGCCTG GATAGAGAGA TACGAGCGGC CCGTGGGCCC GGGGATGCTG
    CCGCAGGACT CGGACTCATG GCCCCAGGCA CCGGATGGGC GAGCTTTGTC CCAGCCGTCC
    CCAGGCAACG ACAGCCCCGA CGGCGACGGC GCCGGTAGCG TGGAGGCCGC TGCGCCACCG
    CCCATCCTCA GCTCGGTGGA GTGGAGCACA TCTGGCGAGC GTTCGGCCAG CGCCCGCTTC
    CCCTCCGCCC GCCCAGGCTC CAGCAGCGAG GAGGACGACG ACGACGACGA GCACGAGGGC
    GTCTTCTCCC AGCCCTTCCT ACCTGCTTCA GATTCTGACA GTGATGTCAT CTTTGAAGAT
    GAGAACAGTA AGAGTCAGAA TCAGGAAGAA GACTGCAATG GGAAGAACAG TTGCCATGAA
    AGTGAGCCAT CGACAACCAC TGAAGCTGTG CACTACCTGT ATATCCAGAT GGAGTACTGT
    GAGAAGAGTA CTTTGCGTGA CACCATTGAC CAGGGGCTAT ATCAAGATAC CATCAGACTC
    TGGAGGCTTT TTCGAGAGAT TCTGGATGGA TTAGCTTATA TCCATGAAAA AGGAATGATT
    CACCGGGATT TGAAGCCTGT CAACATCTTT TTGGATTCTG ATGACCATGT AAAAATAGGT
    GATTTTGGTT TAGCGACAGA CCATCTTGCC TTCACTGCTG ATGGCAAGCA AGATGATACA
    GCAGATCACT TGATTAAATC AGACCCCTCA GGTCATTTGA CTGGGATGGT TGGTACTGCT
    TTATACGTAA GCCCAGAGGT CCAAGGAAGC AGCAGACCCA CGTACAACCA GAAAGTGGAT
    CTCTTCAGCC TGGGAATTAT CTTCTTTGAG ATGTCCTATC ACCCCATGGT CACGGCCTCA
    GAAAGGATCT TTGTTCTCAA CCAACTCAGA GATCCCACTT CACCTAAGTT TCCAGAAGAC
    TTCGATGATG GAGAGCATAC AAAGCAGAAA TCTGTCATCT CCTGGCTGTT GAACCATGAT
    CCCGCCAAGC GGCCCACAGC CACAGAGCTG CTCAAGAGTG AGCTGCTGCC CCCACCCCAG
    ATGGAGGAGT CAGAGTTGCA TGAGGTTCTG CACCACACCC TGGCCAACGT GGATGGGAAG
    GCCTACCGCA CCATGATGGC CCAGATCTTC TCCCAGCGCG TCAGTCCAGC CATCGATTAC
    ACCTATGACA GCAACATGCC GAAGGGCTTT TCAATCCGTA CATCCAAGAT ACAGCAACAT
    GTGTGTGATA CCATCGTCCG CATTTTTAAA AAACACGGAG CTGTCCAGTT GTGTACTCCA
    CTACTGCTTC CCCAAAATAG GCAAATATAC GAGCACAATG AAGCTGCCTT ATTCATGGAC
    CACAGCGGGA TGCTGGTGAT GCTTCCCTTT GACCTACGGG TTCCTTTTGC AAGATATGTG
    GCAAGAAATA ATATATTGAA TTTGAAACGT TACTGCATAG AACGTGTCTT CAGACCTCGC
    AAGTTAGACC GATTTCATCC CAAAGAACTT CTGGAATGTG CATTTGACAT TGTTACGTCT
    ACCACCAACA GCTCTCTGCC CACTGCTGAA GTCATCTACA CGATCTGTGA GATCATCCAG
    GAGTTCCCGG CACTGCAGGA AAGAAATTAC AGTATTTACT TGAACCACAC CATGTTATTG
    AAAGCAATCT TCTTACACTG TGGGATCCCA GAAGATAAAC TCAGTCAAGT CTATGTTATT
    CTATATGATG CAGTGACAGA AAAGCTGACA AGGAGAGAAG TGGAAGCCAA GTTTTGTAAT
    CTGTCTCTGT CATCTAATAG TCTGTGTCGA CTCTACAAAT TCATTGAGCA GAAGGGAGAT
    TTGCAAGATC TAACACCAGC AATAAATTCA TTAATAAAAC AGAAAACAGG TGTTGCCCAA
    CTGGTGAAGT ATGGCTTGAA AGACTTAGAG GAGATCATTG GGCTGTTGAA GAAACTCGGC
    ATCAAGTTAC AGGTCTTGAT CAACTTGGGC TTGGTTTACA AAGTACAGCA GCACAACGGA
    GTCATCTTCC AGTTTGTGGC GGTCATCAGA CGGAGGCAAA GGGCTATTTC AGAAATCCTT
    GCAGCTGGAG GCAGATACGA CCTGCTGATT CCCCAGTTTA GAGGACCGCA GGCTCTGGGA
    CCAGTCCCGA CTGCTGTTGG GGTCAGCATA GCCATAGACA AGATATCTGC AGCTATCCTC
    AACATGGAAG AACCTGTTAC AATAAGCTCT TGTGACCTCC TGATCGTAAG TGTTGGCCAG
    ATGTCCATGT CCAGGGCCAT CAACCTAACC CAGAAACTTT GGTCAGCAGG AATCACAGCA
    CAGATCATGT ACGACTGGTC ACAGTCCCAA GAAGAACTCC AGGAATACTG CAGATACCAC
    GAAATCACCT ACGTGGCCCT CGTCTCAGAT AAAGGAGGAA GCTATGTCAA GGTTAAGTCT
    TTTGAGAAGG AAAGGCAAAC AGAGAAACTT GTGCAGGAGT CTGATCTTAT AGACCACGTT
    TTGCAGAAAC TCAGAACTAA AGTCAGTGAT GAAAGGACTA TCAGAGAAGC TTCTGATAAT
    CTCGCAGTGC AAAATCTGAA GGGGTCCTTT TCTAATGCTT CAGGTTTGTT TGAAATCCAC
    GGAGCAGCAG TTGTCCCCAT TGTGAGCGTG ATAGCCCCAG AGAAGCTGTC AGCCAGCACG
    AGGAGGCGTT ATGAAACTCA GGTACAAACA CGACTTCAGA CCTCCCTTGC CAACTTACAT
    CAGAAAAGCA ATGAAATTGA AATTTTGGCT GTGGATCTAC CCAAAGAAAC AATCTTACAG
    TTTTTATCGT TAGAGTGGGA TGCTGATGAA CAGGCATTTA ACACAACTGT GAAGCAGCTG
    CTGTCACGCC TGCCAAAGCA GAGATACCTC AAATTAGTCT GTGATGAAAT TTATAACATC
    AAAGTAGAGA AAAAGGTATC TGTGCTATTC CTGTATAGCT ACAGAGATGA CTACTACCGA
    ATCTTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28640
    Clone ID Related Accession (Same CDS sequence) XM_005638232.2 , XM_022412709.1
    Accession Version XM_022412709.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product eIF-2-alpha kinase GCN2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTAAACA TCGGACCAAC TCCTCTGGAA GGTCTAGGCG AGAACGTCAG TATTCTCTGT 
    GTAGTAGTGA AGATTCTTCT GGCTCTTGTG AAGTCCTGTA TTTCTCTATG GGTAGCCCTG
    ATCAGCTCAT GGTTTACAAA GGGAGGTGCA TTGATTCAAG GGGCAGAAAC GGAATTCAAC
    TCACTGGTAA AATTGAGCCA TCCAAATGTA GTACGCTACT TTGCAATGAA TCTCAAAGAG
    CAAGATGACT CCATCATGGT GGACATTTTA TTGGAGCACA TTAATGGGGT CTCCCTCTCT
    GCACATCTGA GCCACTCAGG CCCCATCCCT GTGTATCAAC TCCGCAAGTA CACAGCTCAG
    CTCTTGTCTG GCCTCGATTA TTTGCACAGC AATGACGTGG TGCACAAGGT TCTGAATGCA
    TCAAATGTCT TGGTGGATGC AGAGGGCAAT GTCAAGATTA CGGACTACAG CATTTCAAAG
    CGCCTAGCAG ACATTTGCAA GGAGGATGTG TTTGAGCAGA GCCGAGTTAG TTTTAGTGAC
    AGTGCCCTAC CTTATAAAAC GGGGAAGAAA GGGGATGTTT GGTGTCTTGG CCTTCTGCTG
    CTCTTCCTCA GCCAAGGACA AGAATGCGGA GAGTACCCTG TGACCATCCC TAGCGACTTA
    CCAGCTGACT TCCAAGATTT TCTAAAGAAG TGTGTTTGCT TGGATGACAA GGAAAGATGG
    AGTCCCCAGC AATTGTTGAA ACACAGCTTT ATCAATCCAC AGCCGAAAAT GTCTTTATTG
    GAACAGAGTC CTGACGATTC TGGAGGACAA GATTATGTTG AGACTGTTAT TCCTAGTAAG
    CAGCTACCCA GTGCAGCATT CTTCAGTGAG ACACAGAGAC AGTTTTCCCG GTATTTCATT
    GAGTTTGAAG AATTACAGCT TCTCGGCAAA GGAGCCTTCG GAGCTGTCAT CAAGGTGCAG
    AACAAGCTGG ATGGCTGCTG CTACGCGGTG AAGCGCATCC CCATCAATCC GGCCAGTAGG
    CATTTCCGCA GGATCAAGGG CGAGGTGACG CTGCTGTCGC GCCTGCACCA CGAGAACATA
    GTGCGCTACT ATAACGCCTG GATAGAGAGA TACGAGCGGC CCGTGGGCCC GGGGATGCTG
    CCGCAGGACT CGGACTCATG GCCCCAGGCA CCGGATGGGC GAGCTTTGTC CCAGCCGTCC
    CCAGGCAACG ACAGCCCCGA CGGCGACGGC GCCGGTAGCG TGGAGGCCGC TGCGCCACCG
    CCCATCCTCA GCTCGGTGGA GTGGAGCACA TCTGGCGAGC GTTCGGCCAG CGCCCGCTTC
    CCCTCCGCCC GCCCAGGCTC CAGCAGCGAG GAGGACGACG ACGACGACGA GCACGAGGGC
    GTCTTCTCCC AGCCCTTCCT ACCTGCTTCA GATTCTGACA GTGATGTCAT CTTTGAAGAT
    GAGAACAGTA AGAGTCAGAA TCAGGAAGAA GACTGCAATG GGAAGAACAG TTGCCATGAA
    AGTGAGCCAT CGACAACCAC TGAAGCTGTG CACTACCTGT ATATCCAGAT GGAGTACTGT
    GAGAAGAGTA CTTTGCGTGA CACCATTGAC CAGGGGCTAT ATCAAGATAC CATCAGACTC
    TGGAGGCTTT TTCGAGAGAT TCTGGATGGA TTAGCTTATA TCCATGAAAA AGGAATGATT
    CACCGGGATT TGAAGCCTGT CAACATCTTT TTGGATTCTG ATGACCATGT AAAAATAGGT
    GATTTTGGTT TAGCGACAGA CCATCTTGCC TTCACTGCTG ATGGCAAGCA AGATGATACA
    GCAGATCACT TGATTAAATC AGACCCCTCA GGTCATTTGA CTGGGATGGT TGGTACTGCT
    TTATACGTAA GCCCAGAGGT CCAAGGAAGC AGCAGACCCA CGTACAACCA GAAAGTGGAT
    CTCTTCAGCC TGGGAATTAT CTTCTTTGAG ATGTCCTATC ACCCCATGGT CACGGCCTCA
    GAAAGGATCT TTGTTCTCAA CCAACTCAGA GATCCCACTT CACCTAAGTT TCCAGAAGAC
    TTCGATGATG GAGAGCATAC AAAGCAGAAA TCTGTCATCT CCTGGCTGTT GAACCATGAT
    CCCGCCAAGC GGCCCACAGC CACAGAGCTG CTCAAGAGTG AGCTGCTGCC CCCACCCCAG
    ATGGAGGAGT CAGAGTTGCA TGAGGTTCTG CACCACACCC TGGCCAACGT GGATGGGAAG
    GCCTACCGCA CCATGATGGC CCAGATCTTC TCCCAGCGCG TCAGTCCAGC CATCGATTAC
    ACCTATGACA GCAACATGCC GAAGGGCTTT TCAATCCGTA CATCCAAGAT ACAGCAACAT
    GTGTGTGATA CCATCGTCCG CATTTTTAAA AAACACGGAG CTGTCCAGTT GTGTACTCCA
    CTACTGCTTC CCCAAAATAG GCAAATATAC GAGCACAATG AAGCTGCCTT ATTCATGGAC
    CACAGCGGGA TGCTGGTGAT GCTTCCCTTT GACCTACGGG TTCCTTTTGC AAGATATGTG
    GCAAGAAATA ATATATTGAA TTTGAAACGT TACTGCATAG AACGTGTCTT CAGACCTCGC
    AAGTTAGACC GATTTCATCC CAAAGAACTT CTGGAATGTG CATTTGACAT TGTTACGTCT
    ACCACCAACA GCTCTCTGCC CACTGCTGAA GTCATCTACA CGATCTGTGA GATCATCCAG
    GAGTTCCCGG CACTGCAGGA AAGAAATTAC AGTATTTACT TGAACCACAC CATGTTATTG
    AAAGCAATCT TCTTACACTG TGGGATCCCA GAAGATAAAC TCAGTCAAGT CTATGTTATT
    CTATATGATG CAGTGACAGA AAAGCTGACA AGGAGAGAAG TGGAAGCCAA GTTTTGTAAT
    CTGTCTCTGT CATCTAATAG TCTGTGTCGA CTCTACAAAT TCATTGAGCA GAAGGGAGAT
    TTGCAAGATC TAACACCAGC AATAAATTCA TTAATAAAAC AGAAAACAGG TGTTGCCCAA
    CTGGTGAAGT ATGGCTTGAA AGACTTAGAG GAGATCATTG GGCTGTTGAA GAAACTCGGC
    ATCAAGTTAC AGGTCTTGAT CAACTTGGGC TTGGTTTACA AAGTACAGCA GCACAACGGA
    GTCATCTTCC AGTTTGTGGC GGTCATCAGA CGGAGGCAAA GGGCTATTTC AGAAATCCTT
    GCAGCTGGAG GCAGATACGA CCTGCTGATT CCCCAGTTTA GAGGACCGCA GGCTCTGGGA
    CCAGTCCCGA CTGCTGTTGG GGTCAGCATA GCCATAGACA AGATATCTGC AGCTATCCTC
    AACATGGAAG AACCTGTTAC AATAAGCTCT TGTGACCTCC TGATCGTAAG TGTTGGCCAG
    ATGTCCATGT CCAGGGCCAT CAACCTAACC CAGAAACTTT GGTCAGCAGG AATCACAGCA
    CAGATCATGT ACGACTGGTC ACAGTCCCAA GAAGAACTCC AGGAATACTG CAGATACCAC
    GAAATCACCT ACGTGGCCCT CGTCTCAGAT AAAGGAGGAA GCTATGTCAA GGTTAAGTCT
    TTTGAGAAGG AAAGGCAAAC AGAGAAACTT GTGCAGGAGT CTGATCTTAT AGACCACGTT
    TTGCAGAAAC TCAGAACTAA AGTCAGTGAT GAAAGGACTA TCAGAGAAGC TTCTGATAAT
    CTCGCAGTGC AAAATCTGAA GGGGTCCTTT TCTAATGCTT CAGGTTTGTT TGAAATCCAC
    GGAGCAGCAG TTGTCCCCAT TGTGAGCGTG ATAGCCCCAG AGAAGCTGTC AGCCAGCACG
    AGGAGGCGTT ATGAAACTCA GGTACAAACA CGACTTCAGA CCTCCCTTGC CAACTTACAT
    CAGAAAAGCA ATGAAATTGA AATTTTGGCT GTGGATCTAC CCAAAGAAAC AATCTTACAG
    TTTTTATCGT TAGAGTGGGA TGCTGATGAA CAGGCATTTA ACACAACTGT GAAGCAGCTG
    CTGTCACGCC TGCCAAAGCA GAGATACCTC AAATTAGTCT GTGATGAAAT TTATAACATC
    AAAGTAGAGA AAAAGGTATC TGTGCTATTC CTGTATAGCT ACAGAGATGA CTACTACCGA
    ATCTTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268417.1
    CDS194..4285
    Translation

    Target ORF information:

    RefSeq Version XM_022412709.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412709.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGTAAACA TCGGACCAAC TCCTCTGGAA GGTCTAGGCG AGAACGTCAG TATTCTCTGT 
    GTAGTAGTGA AGATTCTTCT GGCTCTTGTG AAGTCCTGTA TTTCTCTATG GGTAGCCCTG
    ATCAGCTCAT GGTTTACAAA GGGAGGTGCA TTGATTCAAG GGGCAGAAAC GGAATTCAAC
    TCACTGGTAA AATTGAGCCA TCCAAATGTA GTACGCTACT TTGCAATGAA TCTCAAAGAG
    CAAGATGACT CCATCATGGT GGACATTTTA TTGGAGCACA TTAATGGGGT CTCCCTCTCT
    GCACATCTGA GCCACTCAGG CCCCATCCCT GTGTATCAAC TCCGCAAGTA CACAGCTCAG
    CTCTTGTCTG GCCTCGATTA TTTGCACAGC AATGACGTGG TGCACAAGGT TCTGAATGCA
    TCAAATGTCT TGGTGGATGC AGAGGGCAAT GTCAAGATTA CGGACTACAG CATTTCAAAG
    CGCCTAGCAG ACATTTGCAA GGAGGATGTG TTTGAGCAGA GCCGAGTTAG TTTTAGTGAC
    AGTGCCCTAC CTTATAAAAC GGGGAAGAAA GGGGATGTTT GGTGTCTTGG CCTTCTGCTG
    CTCTTCCTCA GCCAAGGACA AGAATGCGGA GAGTACCCTG TGACCATCCC TAGCGACTTA
    CCAGCTGACT TCCAAGATTT TCTAAAGAAG TGTGTTTGCT TGGATGACAA GGAAAGATGG
    AGTCCCCAGC AATTGTTGAA ACACAGCTTT ATCAATCCAC AGCCGAAAAT GTCTTTATTG
    GAACAGAGTC CTGACGATTC TGGAGGACAA GATTATGTTG AGACTGTTAT TCCTAGTAAG
    CAGCTACCCA GTGCAGCATT CTTCAGTGAG ACACAGAGAC AGTTTTCCCG GTATTTCATT
    GAGTTTGAAG AATTACAGCT TCTCGGCAAA GGAGCCTTCG GAGCTGTCAT CAAGGTGCAG
    AACAAGCTGG ATGGCTGCTG CTACGCGGTG AAGCGCATCC CCATCAATCC GGCCAGTAGG
    CATTTCCGCA GGATCAAGGG CGAGGTGACG CTGCTGTCGC GCCTGCACCA CGAGAACATA
    GTGCGCTACT ATAACGCCTG GATAGAGAGA TACGAGCGGC CCGTGGGCCC GGGGATGCTG
    CCGCAGGACT CGGACTCATG GCCCCAGGCA CCGGATGGGC GAGCTTTGTC CCAGCCGTCC
    CCAGGCAACG ACAGCCCCGA CGGCGACGGC GCCGGTAGCG TGGAGGCCGC TGCGCCACCG
    CCCATCCTCA GCTCGGTGGA GTGGAGCACA TCTGGCGAGC GTTCGGCCAG CGCCCGCTTC
    CCCTCCGCCC GCCCAGGCTC CAGCAGCGAG GAGGACGACG ACGACGACGA GCACGAGGGC
    GTCTTCTCCC AGCCCTTCCT ACCTGCTTCA GATTCTGACA GTGATGTCAT CTTTGAAGAT
    GAGAACAGTA AGAGTCAGAA TCAGGAAGAA GACTGCAATG GGAAGAACAG TTGCCATGAA
    AGTGAGCCAT CGACAACCAC TGAAGCTGTG CACTACCTGT ATATCCAGAT GGAGTACTGT
    GAGAAGAGTA CTTTGCGTGA CACCATTGAC CAGGGGCTAT ATCAAGATAC CATCAGACTC
    TGGAGGCTTT TTCGAGAGAT TCTGGATGGA TTAGCTTATA TCCATGAAAA AGGAATGATT
    CACCGGGATT TGAAGCCTGT CAACATCTTT TTGGATTCTG ATGACCATGT AAAAATAGGT
    GATTTTGGTT TAGCGACAGA CCATCTTGCC TTCACTGCTG ATGGCAAGCA AGATGATACA
    GCAGATCACT TGATTAAATC AGACCCCTCA GGTCATTTGA CTGGGATGGT TGGTACTGCT
    TTATACGTAA GCCCAGAGGT CCAAGGAAGC AGCAGACCCA CGTACAACCA GAAAGTGGAT
    CTCTTCAGCC TGGGAATTAT CTTCTTTGAG ATGTCCTATC ACCCCATGGT CACGGCCTCA
    GAAAGGATCT TTGTTCTCAA CCAACTCAGA GATCCCACTT CACCTAAGTT TCCAGAAGAC
    TTCGATGATG GAGAGCATAC AAAGCAGAAA TCTGTCATCT CCTGGCTGTT GAACCATGAT
    CCCGCCAAGC GGCCCACAGC CACAGAGCTG CTCAAGAGTG AGCTGCTGCC CCCACCCCAG
    ATGGAGGAGT CAGAGTTGCA TGAGGTTCTG CACCACACCC TGGCCAACGT GGATGGGAAG
    GCCTACCGCA CCATGATGGC CCAGATCTTC TCCCAGCGCG TCAGTCCAGC CATCGATTAC
    ACCTATGACA GCAACATGCC GAAGGGCTTT TCAATCCGTA CATCCAAGAT ACAGCAACAT
    GTGTGTGATA CCATCGTCCG CATTTTTAAA AAACACGGAG CTGTCCAGTT GTGTACTCCA
    CTACTGCTTC CCCAAAATAG GCAAATATAC GAGCACAATG AAGCTGCCTT ATTCATGGAC
    CACAGCGGGA TGCTGGTGAT GCTTCCCTTT GACCTACGGG TTCCTTTTGC AAGATATGTG
    GCAAGAAATA ATATATTGAA TTTGAAACGT TACTGCATAG AACGTGTCTT CAGACCTCGC
    AAGTTAGACC GATTTCATCC CAAAGAACTT CTGGAATGTG CATTTGACAT TGTTACGTCT
    ACCACCAACA GCTCTCTGCC CACTGCTGAA GTCATCTACA CGATCTGTGA GATCATCCAG
    GAGTTCCCGG CACTGCAGGA AAGAAATTAC AGTATTTACT TGAACCACAC CATGTTATTG
    AAAGCAATCT TCTTACACTG TGGGATCCCA GAAGATAAAC TCAGTCAAGT CTATGTTATT
    CTATATGATG CAGTGACAGA AAAGCTGACA AGGAGAGAAG TGGAAGCCAA GTTTTGTAAT
    CTGTCTCTGT CATCTAATAG TCTGTGTCGA CTCTACAAAT TCATTGAGCA GAAGGGAGAT
    TTGCAAGATC TAACACCAGC AATAAATTCA TTAATAAAAC AGAAAACAGG TGTTGCCCAA
    CTGGTGAAGT ATGGCTTGAA AGACTTAGAG GAGATCATTG GGCTGTTGAA GAAACTCGGC
    ATCAAGTTAC AGGTCTTGAT CAACTTGGGC TTGGTTTACA AAGTACAGCA GCACAACGGA
    GTCATCTTCC AGTTTGTGGC GGTCATCAGA CGGAGGCAAA GGGCTATTTC AGAAATCCTT
    GCAGCTGGAG GCAGATACGA CCTGCTGATT CCCCAGTTTA GAGGACCGCA GGCTCTGGGA
    CCAGTCCCGA CTGCTGTTGG GGTCAGCATA GCCATAGACA AGATATCTGC AGCTATCCTC
    AACATGGAAG AACCTGTTAC AATAAGCTCT TGTGACCTCC TGATCGTAAG TGTTGGCCAG
    ATGTCCATGT CCAGGGCCAT CAACCTAACC CAGAAACTTT GGTCAGCAGG AATCACAGCA
    CAGATCATGT ACGACTGGTC ACAGTCCCAA GAAGAACTCC AGGAATACTG CAGATACCAC
    GAAATCACCT ACGTGGCCCT CGTCTCAGAT AAAGGAGGAA GCTATGTCAA GGTTAAGTCT
    TTTGAGAAGG AAAGGCAAAC AGAGAAACTT GTGCAGGAGT CTGATCTTAT AGACCACGTT
    TTGCAGAAAC TCAGAACTAA AGTCAGTGAT GAAAGGACTA TCAGAGAAGC TTCTGATAAT
    CTCGCAGTGC AAAATCTGAA GGGGTCCTTT TCTAATGCTT CAGGTTTGTT TGAAATCCAC
    GGAGCAGCAG TTGTCCCCAT TGTGAGCGTG ATAGCCCCAG AGAAGCTGTC AGCCAGCACG
    AGGAGGCGTT ATGAAACTCA GGTACAAACA CGACTTCAGA CCTCCCTTGC CAACTTACAT
    CAGAAAAGCA ATGAAATTGA AATTTTGGCT GTGGATCTAC CCAAAGAAAC AATCTTACAG
    TTTTTATCGT TAGAGTGGGA TGCTGATGAA CAGGCATTTA ACACAACTGT GAAGCAGCTG
    CTGTCACGCC TGCCAAAGCA GAGATACCTC AAATTAGTCT GTGATGAAAT TTATAACATC
    AAAGTAGAGA AAAAGGTATC TGTGCTATTC CTGTATAGCT ACAGAGATGA CTACTACCGA
    ATCTTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28636
    Clone ID Related Accession (Same CDS sequence) XM_535431.5 , XM_535431.6
    Accession Version XM_535431.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product eukaryotic translation initiation factor 2-alpha kinase 4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_535431.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
    CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC
    CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA
    CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT
    GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG
    GTGATGATAT TTGAACTGGC CGACCATGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC
    CCTCCCAAGT CTTTCCATGA GGAAATGCTA GAAAGGCGGG CTCAGGAGGA GCAGCGGAGG
    CTGTTGGAGG CCAAGCAGAA GGTAGAGCAG GAGCAACGTG AAATCCTTCA TGAGATTCAA
    AGAAGGAAAG AAGAGATAAA AGAAGAGAGA AAAAGAAAAG AAATGGCTAA GCAGGAGCGT
    TTGGAAATTG CTAGTTTGTC AAACCAAGAT CAAAACTCTA GGAAAGACCC AGGAGGACAC
    AGAATATCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAGGCGAGAA CGTCAGTATT CTCTGTGTAG TAGTGAAGAT
    TCTTCTGGCT CTTGTGAAGT CCTGTATTTC TCTATGGGTA GCCCTGATCA GCTCATGGTT
    TACAAAGGGA GGTGCATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA TAATGCTTTG
    GAAACGGCCA CTGGCAACTT TGTGTCATTA CATGAGTGGG TCCTTCGGTG GCAGAAAAAA
    ATGGGCCCGT TCCTTACCAG TCAAGAGAAG GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACGGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC
    TACTTTGCAA TGAATCTCAA AGAGCAAGAT GACTCCATCA TGGTGGACAT TTTATTGGAG
    CACATTAATG GGGTCTCCCT CTCTGCACAT CTGAGCCACT CAGGCCCCAT CCCTGTGTAT
    CAACTCCGCA AGTACACAGC TCAGCTCTTG TCTGGCCTCG ATTATTTGCA CAGCAATGAC
    GTGGTGCACA AGGTTCTGAA TGCATCAAAT GTCTTGGTGG ATGCAGAGGG CAATGTCAAG
    ATTACGGACT ACAGCATTTC AAAGCGCCTA GCAGACATTT GCAAGGAGGA TGTGTTTGAG
    CAGAGCCGAG TTAGTTTTAG TGACAGTGCC CTACCTTATA AAACGGGGAA GAAAGGGGAT
    GTTTGGTGTC TTGGCCTTCT GCTGCTCTTC CTCAGCCAAG GACAAGAATG CGGAGAGTAC
    CCTGTGACCA TCCCTAGCGA CTTACCAGCT GACTTCCAAG ATTTTCTAAA GAAGTGTGTT
    TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAATTGT TGAAACACAG CTTTATCAAT
    CCACAGCCGA AAATGTCTTT ATTGGAACAG AGTCCTGACG ATTCTGGAGG ACAAGATTAT
    GTTGAGACTG TTATTCCTAG TAAGCAGCTA CCCAGTGCAG CATTCTTCAG TGAGACACAG
    AGACAGTTTT CCCGGTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCC
    TTCGGAGCTG TCATCAAGGT GCAGAACAAG CTGGATGGCT GCTGCTACGC GGTGAAGCGC
    ATCCCCATCA ATCCGGCCAG TAGGCATTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTGC ACCACGAGAA CATAGTGCGC TACTATAACG CCTGGATAGA GAGATACGAG
    CGGCCCGTGG GCCCGGGGAT GCTGCCGCAG GACTCGGACT CATGGCCCCA GGCACCGGAT
    GGGCGAGCTT TGTCCCAGCC GTCCCCAGGC AACGACAGCC CCGACGGCGA CGGCGCCGGT
    AGCGTGGAGG CCGCTGCGCC ACCGCCCATC CTCAGCTCGG TGGAGTGGAG CACATCTGGC
    GAGCGTTCGG CCAGCGCCCG CTTCCCCTCC GCCCGCCCAG GCTCCAGCAG CGAGGAGGAC
    GACGACGACG ACGAGCACGA GGGCGTCTTC TCCCAGCCCT TCCTACCTGC TTCAGATTCT
    GACAGTGATG TCATCTTTGA AGATGAGAAC AGTAAGAGTC AGAATCAGGA AGAAGACTGC
    AATGGGAAGA ACAGTTGCCA TGAAAGTGAG CCATCGACAA CCACTGAAGC TGTGCACTAC
    CTGTATATCC AGATGGAGTA CTGTGAGAAG AGTACTTTGC GTGACACCAT TGACCAGGGG
    CTATATCAAG ATACCATCAG ACTCTGGAGG CTTTTTCGAG AGATTCTGGA TGGATTAGCT
    TATATCCATG AAAAAGGAAT GATTCACCGG GATTTGAAGC CTGTCAACAT CTTTTTGGAT
    TCTGATGACC ATGTAAAAAT AGGTGATTTT GGTTTAGCGA CAGACCATCT TGCCTTCACT
    GCTGATGGCA AGCAAGATGA TACAGCAGAT CACTTGATTA AATCAGACCC CTCAGGTCAT
    TTGACTGGGA TGGTTGGTAC TGCTTTATAC GTAAGCCCAG AGGTCCAAGG AAGCAGCAGA
    CCCACGTACA ACCAGAAAGT GGATCTCTTC AGCCTGGGAA TTATCTTCTT TGAGATGTCC
    TATCACCCCA TGGTCACGGC CTCAGAAAGG ATCTTTGTTC TCAACCAACT CAGAGATCCC
    ACTTCACCTA AGTTTCCAGA AGACTTCGAT GATGGAGAGC ATACAAAGCA GAAATCTGTC
    ATCTCCTGGC TGTTGAACCA TGATCCCGCC AAGCGGCCCA CAGCCACAGA GCTGCTCAAG
    AGTGAGCTGC TGCCCCCACC CCAGATGGAG GAGTCAGAGT TGCATGAGGT TCTGCACCAC
    ACCCTGGCCA ACGTGGATGG GAAGGCCTAC CGCACCATGA TGGCCCAGAT CTTCTCCCAG
    CGCGTCAGTC CAGCCATCGA TTACACCTAT GACAGCAACA TGCCGAAGGG CTTTTCAATC
    CGTACATCCA AGATACAGCA ACATGTGTGT GATACCATCG TCCGCATTTT TAAAAAACAC
    GGAGCTGTCC AGTTGTGTAC TCCACTACTG CTTCCCCAAA ATAGGCAAAT ATACGAGCAC
    AATGAAGCTG CCTTATTCAT GGACCACAGC GGGATGCTGG TGATGCTTCC CTTTGACCTA
    CGGGTTCCTT TTGCAAGATA TGTGGCAAGA AATAATATAT TGAATTTGAA ACGTTACTGC
    ATAGAACGTG TCTTCAGACC TCGCAAGTTA GACCGATTTC ATCCCAAAGA ACTTCTGGAA
    TGTGCATTTG ACATTGTTAC GTCTACCACC AACAGCTCTC TGCCCACTGC TGAAGTCATC
    TACACGATCT GTGAGATCAT CCAGGAGTTC CCGGCACTGC AGGAAAGAAA TTACAGTATT
    TACTTGAACC ACACCATGTT ATTGAAAGCA ATCTTCTTAC ACTGTGGGAT CCCAGAAGAT
    AAACTCAGTC AAGTCTATGT TATTCTATAT GATGCAGTGA CAGAAAAGCT GACAAGGAGA
    GAAGTGGAAG CCAAGTTTTG TAATCTGTCT CTGTCATCTA ATAGTCTGTG TCGACTCTAC
    AAATTCATTG AGCAGAAGGG AGATTTGCAA GATCTAACAC CAGCAATAAA TTCATTAATA
    AAACAGAAAA CAGGTGTTGC CCAACTGGTG AAGTATGGCT TGAAAGACTT AGAGGAGATC
    ATTGGGCTGT TGAAGAAACT CGGCATCAAG TTACAGGTCT TGATCAACTT GGGCTTGGTT
    TACAAAGTAC AGCAGCACAA CGGAGTCATC TTCCAGTTTG TGGCGGTCAT CAGACGGAGG
    CAAAGGGCTA TTTCAGAAAT CCTTGCAGCT GGAGGCAGAT ACGACCTGCT GATTCCCCAG
    TTTAGAGGAC CGCAGGCTCT GGGACCAGTC CCGACTGCTG TTGGGGTCAG CATAGCCATA
    GACAAGATAT CTGCAGCTAT CCTCAACATG GAAGAACCTG TTACAATAAG CTCTTGTGAC
    CTCCTGATCG TAAGTGTTGG CCAGATGTCC ATGTCCAGGG CCATCAACCT AACCCAGAAA
    CTTTGGTCAG CAGGAATCAC AGCACAGATC ATGTACGACT GGTCACAGTC CCAAGAAGAA
    CTCCAGGAAT ACTGCAGATA CCACGAAATC ACCTACGTGG CCCTCGTCTC AGATAAAGGA
    GGAAGCTATG TCAAGGTTAA GTCTTTTGAG AAGGAAAGGC AAACAGAGAA ACTTGTGCAG
    GAGTCTGATC TTATAGACCA CGTTTTGCAG AAACTCAGAA CTAAAGTCAG TGATGAAAGG
    ACTATCAGAG AAGCTTCTGA TAATCTCGCA GTGCAAAATC TGAAGGGGTC CTTTTCTAAT
    GCTTCAGGTT TGTTTGAAAT CCACGGAGCA GCAGTTGTCC CCATTGTGAG CGTGATAGCC
    CCAGAGAAGC TGTCAGCCAG CACGAGGAGG CGTTATGAAA CTCAGGTACA AACACGACTT
    CAGACCTCCC TTGCCAACTT ACATCAGAAA AGCAATGAAA TTGAAATTTT GGCTGTGGAT
    CTACCCAAAG AAACAATCTT ACAGTTTTTA TCGTTAGAGT GGGATGCTGA TGAACAGGCA
    TTTAACACAA CTGTGAAGCA GCTGCTGTCA CGCCTGCCAA AGCAGAGATA CCTCAAATTA
    GTCTGTGATG AAATTTATAA CATCAAAGTA GAGAAAAAGG TATCTGTGCT ATTCCTGTAT
    AGCTACAGAG ATGACTACTA CCGAATCTTA TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_535431.3
    CDS91..5046
    Translation

    Target ORF information:

    RefSeq Version XM_535431.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_535431.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
    CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC
    CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA
    CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT
    GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG
    GTGATGATAT TTGAACTGGC CGACCATGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC
    CCTCCCAAGT CTTTCCATGA GGAAATGCTA GAAAGGCGGG CTCAGGAGGA GCAGCGGAGG
    CTGTTGGAGG CCAAGCAGAA GGTAGAGCAG GAGCAACGTG AAATCCTTCA TGAGATTCAA
    AGAAGGAAAG AAGAGATAAA AGAAGAGAGA AAAAGAAAAG AAATGGCTAA GCAGGAGCGT
    TTGGAAATTG CTAGTTTGTC AAACCAAGAT CAAAACTCTA GGAAAGACCC AGGAGGACAC
    AGAATATCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAGGCGAGAA CGTCAGTATT CTCTGTGTAG TAGTGAAGAT
    TCTTCTGGCT CTTGTGAAGT CCTGTATTTC TCTATGGGTA GCCCTGATCA GCTCATGGTT
    TACAAAGGGA GGTGCATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA TAATGCTTTG
    GAAACGGCCA CTGGCAACTT TGTGTCATTA CATGAGTGGG TCCTTCGGTG GCAGAAAAAA
    ATGGGCCCGT TCCTTACCAG TCAAGAGAAG GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACGGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC
    TACTTTGCAA TGAATCTCAA AGAGCAAGAT GACTCCATCA TGGTGGACAT TTTATTGGAG
    CACATTAATG GGGTCTCCCT CTCTGCACAT CTGAGCCACT CAGGCCCCAT CCCTGTGTAT
    CAACTCCGCA AGTACACAGC TCAGCTCTTG TCTGGCCTCG ATTATTTGCA CAGCAATGAC
    GTGGTGCACA AGGTTCTGAA TGCATCAAAT GTCTTGGTGG ATGCAGAGGG CAATGTCAAG
    ATTACGGACT ACAGCATTTC AAAGCGCCTA GCAGACATTT GCAAGGAGGA TGTGTTTGAG
    CAGAGCCGAG TTAGTTTTAG TGACAGTGCC CTACCTTATA AAACGGGGAA GAAAGGGGAT
    GTTTGGTGTC TTGGCCTTCT GCTGCTCTTC CTCAGCCAAG GACAAGAATG CGGAGAGTAC
    CCTGTGACCA TCCCTAGCGA CTTACCAGCT GACTTCCAAG ATTTTCTAAA GAAGTGTGTT
    TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAATTGT TGAAACACAG CTTTATCAAT
    CCACAGCCGA AAATGTCTTT ATTGGAACAG AGTCCTGACG ATTCTGGAGG ACAAGATTAT
    GTTGAGACTG TTATTCCTAG TAAGCAGCTA CCCAGTGCAG CATTCTTCAG TGAGACACAG
    AGACAGTTTT CCCGGTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCC
    TTCGGAGCTG TCATCAAGGT GCAGAACAAG CTGGATGGCT GCTGCTACGC GGTGAAGCGC
    ATCCCCATCA ATCCGGCCAG TAGGCATTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTGC ACCACGAGAA CATAGTGCGC TACTATAACG CCTGGATAGA GAGATACGAG
    CGGCCCGTGG GCCCGGGGAT GCTGCCGCAG GACTCGGACT CATGGCCCCA GGCACCGGAT
    GGGCGAGCTT TGTCCCAGCC GTCCCCAGGC AACGACAGCC CCGACGGCGA CGGCGCCGGT
    AGCGTGGAGG CCGCTGCGCC ACCGCCCATC CTCAGCTCGG TGGAGTGGAG CACATCTGGC
    GAGCGTTCGG CCAGCGCCCG CTTCCCCTCC GCCCGCCCAG GCTCCAGCAG CGAGGAGGAC
    GACGACGACG ACGAGCACGA GGGCGTCTTC TCCCAGCCCT TCCTACCTGC TTCAGATTCT
    GACAGTGATG TCATCTTTGA AGATGAGAAC AGTAAGAGTC AGAATCAGGA AGAAGACTGC
    AATGGGAAGA ACAGTTGCCA TGAAAGTGAG CCATCGACAA CCACTGAAGC TGTGCACTAC
    CTGTATATCC AGATGGAGTA CTGTGAGAAG AGTACTTTGC GTGACACCAT TGACCAGGGG
    CTATATCAAG ATACCATCAG ACTCTGGAGG CTTTTTCGAG AGATTCTGGA TGGATTAGCT
    TATATCCATG AAAAAGGAAT GATTCACCGG GATTTGAAGC CTGTCAACAT CTTTTTGGAT
    TCTGATGACC ATGTAAAAAT AGGTGATTTT GGTTTAGCGA CAGACCATCT TGCCTTCACT
    GCTGATGGCA AGCAAGATGA TACAGCAGAT CACTTGATTA AATCAGACCC CTCAGGTCAT
    TTGACTGGGA TGGTTGGTAC TGCTTTATAC GTAAGCCCAG AGGTCCAAGG AAGCAGCAGA
    CCCACGTACA ACCAGAAAGT GGATCTCTTC AGCCTGGGAA TTATCTTCTT TGAGATGTCC
    TATCACCCCA TGGTCACGGC CTCAGAAAGG ATCTTTGTTC TCAACCAACT CAGAGATCCC
    ACTTCACCTA AGTTTCCAGA AGACTTCGAT GATGGAGAGC ATACAAAGCA GAAATCTGTC
    ATCTCCTGGC TGTTGAACCA TGATCCCGCC AAGCGGCCCA CAGCCACAGA GCTGCTCAAG
    AGTGAGCTGC TGCCCCCACC CCAGATGGAG GAGTCAGAGT TGCATGAGGT TCTGCACCAC
    ACCCTGGCCA ACGTGGATGG GAAGGCCTAC CGCACCATGA TGGCCCAGAT CTTCTCCCAG
    CGCGTCAGTC CAGCCATCGA TTACACCTAT GACAGCAACA TGCCGAAGGG CTTTTCAATC
    CGTACATCCA AGATACAGCA ACATGTGTGT GATACCATCG TCCGCATTTT TAAAAAACAC
    GGAGCTGTCC AGTTGTGTAC TCCACTACTG CTTCCCCAAA ATAGGCAAAT ATACGAGCAC
    AATGAAGCTG CCTTATTCAT GGACCACAGC GGGATGCTGG TGATGCTTCC CTTTGACCTA
    CGGGTTCCTT TTGCAAGATA TGTGGCAAGA AATAATATAT TGAATTTGAA ACGTTACTGC
    ATAGAACGTG TCTTCAGACC TCGCAAGTTA GACCGATTTC ATCCCAAAGA ACTTCTGGAA
    TGTGCATTTG ACATTGTTAC GTCTACCACC AACAGCTCTC TGCCCACTGC TGAAGTCATC
    TACACGATCT GTGAGATCAT CCAGGAGTTC CCGGCACTGC AGGAAAGAAA TTACAGTATT
    TACTTGAACC ACACCATGTT ATTGAAAGCA ATCTTCTTAC ACTGTGGGAT CCCAGAAGAT
    AAACTCAGTC AAGTCTATGT TATTCTATAT GATGCAGTGA CAGAAAAGCT GACAAGGAGA
    GAAGTGGAAG CCAAGTTTTG TAATCTGTCT CTGTCATCTA ATAGTCTGTG TCGACTCTAC
    AAATTCATTG AGCAGAAGGG AGATTTGCAA GATCTAACAC CAGCAATAAA TTCATTAATA
    AAACAGAAAA CAGGTGTTGC CCAACTGGTG AAGTATGGCT TGAAAGACTT AGAGGAGATC
    ATTGGGCTGT TGAAGAAACT CGGCATCAAG TTACAGGTCT TGATCAACTT GGGCTTGGTT
    TACAAAGTAC AGCAGCACAA CGGAGTCATC TTCCAGTTTG TGGCGGTCAT CAGACGGAGG
    CAAAGGGCTA TTTCAGAAAT CCTTGCAGCT GGAGGCAGAT ACGACCTGCT GATTCCCCAG
    TTTAGAGGAC CGCAGGCTCT GGGACCAGTC CCGACTGCTG TTGGGGTCAG CATAGCCATA
    GACAAGATAT CTGCAGCTAT CCTCAACATG GAAGAACCTG TTACAATAAG CTCTTGTGAC
    CTCCTGATCG TAAGTGTTGG CCAGATGTCC ATGTCCAGGG CCATCAACCT AACCCAGAAA
    CTTTGGTCAG CAGGAATCAC AGCACAGATC ATGTACGACT GGTCACAGTC CCAAGAAGAA
    CTCCAGGAAT ACTGCAGATA CCACGAAATC ACCTACGTGG CCCTCGTCTC AGATAAAGGA
    GGAAGCTATG TCAAGGTTAA GTCTTTTGAG AAGGAAAGGC AAACAGAGAA ACTTGTGCAG
    GAGTCTGATC TTATAGACCA CGTTTTGCAG AAACTCAGAA CTAAAGTCAG TGATGAAAGG
    ACTATCAGAG AAGCTTCTGA TAATCTCGCA GTGCAAAATC TGAAGGGGTC CTTTTCTAAT
    GCTTCAGGTT TGTTTGAAAT CCACGGAGCA GCAGTTGTCC CCATTGTGAG CGTGATAGCC
    CCAGAGAAGC TGTCAGCCAG CACGAGGAGG CGTTATGAAA CTCAGGTACA AACACGACTT
    CAGACCTCCC TTGCCAACTT ACATCAGAAA AGCAATGAAA TTGAAATTTT GGCTGTGGAT
    CTACCCAAAG AAACAATCTT ACAGTTTTTA TCGTTAGAGT GGGATGCTGA TGAACAGGCA
    TTTAACACAA CTGTGAAGCA GCTGCTGTCA CGCCTGCCAA AGCAGAGATA CCTCAAATTA
    GTCTGTGATG AAATTTATAA CATCAAAGTA GAGAAAAAGG TATCTGTGCT ATTCCTGTAT
    AGCTACAGAG ATGACTACTA CCGAATCTTA TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28636
    Clone ID Related Accession (Same CDS sequence) XM_535431.5 , XM_535431.6
    Accession Version XM_535431.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product eIF-2-alpha kinase GCN2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_535431.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
    CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC
    CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA
    CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT
    GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG
    GTGATGATAT TTGAACTGGC CGACCATGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC
    CCTCCCAAGT CTTTCCATGA GGAAATGCTA GAAAGGCGGG CTCAGGAGGA GCAGCGGAGG
    CTGTTGGAGG CCAAGCAGAA GGTAGAGCAG GAGCAACGTG AAATCCTTCA TGAGATTCAA
    AGAAGGAAAG AAGAGATAAA AGAAGAGAGA AAAAGAAAAG AAATGGCTAA GCAGGAGCGT
    TTGGAAATTG CTAGTTTGTC AAACCAAGAT CAAAACTCTA GGAAAGACCC AGGAGGACAC
    AGAATATCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAGGCGAGAA CGTCAGTATT CTCTGTGTAG TAGTGAAGAT
    TCTTCTGGCT CTTGTGAAGT CCTGTATTTC TCTATGGGTA GCCCTGATCA GCTCATGGTT
    TACAAAGGGA GGTGCATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA TAATGCTTTG
    GAAACGGCCA CTGGCAACTT TGTGTCATTA CATGAGTGGG TCCTTCGGTG GCAGAAAAAA
    ATGGGCCCGT TCCTTACCAG TCAAGAGAAG GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACGGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC
    TACTTTGCAA TGAATCTCAA AGAGCAAGAT GACTCCATCA TGGTGGACAT TTTATTGGAG
    CACATTAATG GGGTCTCCCT CTCTGCACAT CTGAGCCACT CAGGCCCCAT CCCTGTGTAT
    CAACTCCGCA AGTACACAGC TCAGCTCTTG TCTGGCCTCG ATTATTTGCA CAGCAATGAC
    GTGGTGCACA AGGTTCTGAA TGCATCAAAT GTCTTGGTGG ATGCAGAGGG CAATGTCAAG
    ATTACGGACT ACAGCATTTC AAAGCGCCTA GCAGACATTT GCAAGGAGGA TGTGTTTGAG
    CAGAGCCGAG TTAGTTTTAG TGACAGTGCC CTACCTTATA AAACGGGGAA GAAAGGGGAT
    GTTTGGTGTC TTGGCCTTCT GCTGCTCTTC CTCAGCCAAG GACAAGAATG CGGAGAGTAC
    CCTGTGACCA TCCCTAGCGA CTTACCAGCT GACTTCCAAG ATTTTCTAAA GAAGTGTGTT
    TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAATTGT TGAAACACAG CTTTATCAAT
    CCACAGCCGA AAATGTCTTT ATTGGAACAG AGTCCTGACG ATTCTGGAGG ACAAGATTAT
    GTTGAGACTG TTATTCCTAG TAAGCAGCTA CCCAGTGCAG CATTCTTCAG TGAGACACAG
    AGACAGTTTT CCCGGTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCC
    TTCGGAGCTG TCATCAAGGT GCAGAACAAG CTGGATGGCT GCTGCTACGC GGTGAAGCGC
    ATCCCCATCA ATCCGGCCAG TAGGCATTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTGC ACCACGAGAA CATAGTGCGC TACTATAACG CCTGGATAGA GAGATACGAG
    CGGCCCGTGG GCCCGGGGAT GCTGCCGCAG GACTCGGACT CATGGCCCCA GGCACCGGAT
    GGGCGAGCTT TGTCCCAGCC GTCCCCAGGC AACGACAGCC CCGACGGCGA CGGCGCCGGT
    AGCGTGGAGG CCGCTGCGCC ACCGCCCATC CTCAGCTCGG TGGAGTGGAG CACATCTGGC
    GAGCGTTCGG CCAGCGCCCG CTTCCCCTCC GCCCGCCCAG GCTCCAGCAG CGAGGAGGAC
    GACGACGACG ACGAGCACGA GGGCGTCTTC TCCCAGCCCT TCCTACCTGC TTCAGATTCT
    GACAGTGATG TCATCTTTGA AGATGAGAAC AGTAAGAGTC AGAATCAGGA AGAAGACTGC
    AATGGGAAGA ACAGTTGCCA TGAAAGTGAG CCATCGACAA CCACTGAAGC TGTGCACTAC
    CTGTATATCC AGATGGAGTA CTGTGAGAAG AGTACTTTGC GTGACACCAT TGACCAGGGG
    CTATATCAAG ATACCATCAG ACTCTGGAGG CTTTTTCGAG AGATTCTGGA TGGATTAGCT
    TATATCCATG AAAAAGGAAT GATTCACCGG GATTTGAAGC CTGTCAACAT CTTTTTGGAT
    TCTGATGACC ATGTAAAAAT AGGTGATTTT GGTTTAGCGA CAGACCATCT TGCCTTCACT
    GCTGATGGCA AGCAAGATGA TACAGCAGAT CACTTGATTA AATCAGACCC CTCAGGTCAT
    TTGACTGGGA TGGTTGGTAC TGCTTTATAC GTAAGCCCAG AGGTCCAAGG AAGCAGCAGA
    CCCACGTACA ACCAGAAAGT GGATCTCTTC AGCCTGGGAA TTATCTTCTT TGAGATGTCC
    TATCACCCCA TGGTCACGGC CTCAGAAAGG ATCTTTGTTC TCAACCAACT CAGAGATCCC
    ACTTCACCTA AGTTTCCAGA AGACTTCGAT GATGGAGAGC ATACAAAGCA GAAATCTGTC
    ATCTCCTGGC TGTTGAACCA TGATCCCGCC AAGCGGCCCA CAGCCACAGA GCTGCTCAAG
    AGTGAGCTGC TGCCCCCACC CCAGATGGAG GAGTCAGAGT TGCATGAGGT TCTGCACCAC
    ACCCTGGCCA ACGTGGATGG GAAGGCCTAC CGCACCATGA TGGCCCAGAT CTTCTCCCAG
    CGCGTCAGTC CAGCCATCGA TTACACCTAT GACAGCAACA TGCCGAAGGG CTTTTCAATC
    CGTACATCCA AGATACAGCA ACATGTGTGT GATACCATCG TCCGCATTTT TAAAAAACAC
    GGAGCTGTCC AGTTGTGTAC TCCACTACTG CTTCCCCAAA ATAGGCAAAT ATACGAGCAC
    AATGAAGCTG CCTTATTCAT GGACCACAGC GGGATGCTGG TGATGCTTCC CTTTGACCTA
    CGGGTTCCTT TTGCAAGATA TGTGGCAAGA AATAATATAT TGAATTTGAA ACGTTACTGC
    ATAGAACGTG TCTTCAGACC TCGCAAGTTA GACCGATTTC ATCCCAAAGA ACTTCTGGAA
    TGTGCATTTG ACATTGTTAC GTCTACCACC AACAGCTCTC TGCCCACTGC TGAAGTCATC
    TACACGATCT GTGAGATCAT CCAGGAGTTC CCGGCACTGC AGGAAAGAAA TTACAGTATT
    TACTTGAACC ACACCATGTT ATTGAAAGCA ATCTTCTTAC ACTGTGGGAT CCCAGAAGAT
    AAACTCAGTC AAGTCTATGT TATTCTATAT GATGCAGTGA CAGAAAAGCT GACAAGGAGA
    GAAGTGGAAG CCAAGTTTTG TAATCTGTCT CTGTCATCTA ATAGTCTGTG TCGACTCTAC
    AAATTCATTG AGCAGAAGGG AGATTTGCAA GATCTAACAC CAGCAATAAA TTCATTAATA
    AAACAGAAAA CAGGTGTTGC CCAACTGGTG AAGTATGGCT TGAAAGACTT AGAGGAGATC
    ATTGGGCTGT TGAAGAAACT CGGCATCAAG TTACAGGTCT TGATCAACTT GGGCTTGGTT
    TACAAAGTAC AGCAGCACAA CGGAGTCATC TTCCAGTTTG TGGCGGTCAT CAGACGGAGG
    CAAAGGGCTA TTTCAGAAAT CCTTGCAGCT GGAGGCAGAT ACGACCTGCT GATTCCCCAG
    TTTAGAGGAC CGCAGGCTCT GGGACCAGTC CCGACTGCTG TTGGGGTCAG CATAGCCATA
    GACAAGATAT CTGCAGCTAT CCTCAACATG GAAGAACCTG TTACAATAAG CTCTTGTGAC
    CTCCTGATCG TAAGTGTTGG CCAGATGTCC ATGTCCAGGG CCATCAACCT AACCCAGAAA
    CTTTGGTCAG CAGGAATCAC AGCACAGATC ATGTACGACT GGTCACAGTC CCAAGAAGAA
    CTCCAGGAAT ACTGCAGATA CCACGAAATC ACCTACGTGG CCCTCGTCTC AGATAAAGGA
    GGAAGCTATG TCAAGGTTAA GTCTTTTGAG AAGGAAAGGC AAACAGAGAA ACTTGTGCAG
    GAGTCTGATC TTATAGACCA CGTTTTGCAG AAACTCAGAA CTAAAGTCAG TGATGAAAGG
    ACTATCAGAG AAGCTTCTGA TAATCTCGCA GTGCAAAATC TGAAGGGGTC CTTTTCTAAT
    GCTTCAGGTT TGTTTGAAAT CCACGGAGCA GCAGTTGTCC CCATTGTGAG CGTGATAGCC
    CCAGAGAAGC TGTCAGCCAG CACGAGGAGG CGTTATGAAA CTCAGGTACA AACACGACTT
    CAGACCTCCC TTGCCAACTT ACATCAGAAA AGCAATGAAA TTGAAATTTT GGCTGTGGAT
    CTACCCAAAG AAACAATCTT ACAGTTTTTA TCGTTAGAGT GGGATGCTGA TGAACAGGCA
    TTTAACACAA CTGTGAAGCA GCTGCTGTCA CGCCTGCCAA AGCAGAGATA CCTCAAATTA
    GTCTGTGATG AAATTTATAA CATCAAAGTA GAGAAAAAGG TATCTGTGCT ATTCCTGTAT
    AGCTACAGAG ATGACTACTA CCGAATCTTA TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_535431.3
    CDS1..4956
    Translation

    Target ORF information:

    RefSeq Version XM_535431.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_535431.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
    CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC
    CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA
    CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT
    GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG
    GTGATGATAT TTGAACTGGC CGACCATGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC
    CCTCCCAAGT CTTTCCATGA GGAAATGCTA GAAAGGCGGG CTCAGGAGGA GCAGCGGAGG
    CTGTTGGAGG CCAAGCAGAA GGTAGAGCAG GAGCAACGTG AAATCCTTCA TGAGATTCAA
    AGAAGGAAAG AAGAGATAAA AGAAGAGAGA AAAAGAAAAG AAATGGCTAA GCAGGAGCGT
    TTGGAAATTG CTAGTTTGTC AAACCAAGAT CAAAACTCTA GGAAAGACCC AGGAGGACAC
    AGAATATCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAGGCGAGAA CGTCAGTATT CTCTGTGTAG TAGTGAAGAT
    TCTTCTGGCT CTTGTGAAGT CCTGTATTTC TCTATGGGTA GCCCTGATCA GCTCATGGTT
    TACAAAGGGA GGTGCATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA TAATGCTTTG
    GAAACGGCCA CTGGCAACTT TGTGTCATTA CATGAGTGGG TCCTTCGGTG GCAGAAAAAA
    ATGGGCCCGT TCCTTACCAG TCAAGAGAAG GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACGGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC
    TACTTTGCAA TGAATCTCAA AGAGCAAGAT GACTCCATCA TGGTGGACAT TTTATTGGAG
    CACATTAATG GGGTCTCCCT CTCTGCACAT CTGAGCCACT CAGGCCCCAT CCCTGTGTAT
    CAACTCCGCA AGTACACAGC TCAGCTCTTG TCTGGCCTCG ATTATTTGCA CAGCAATGAC
    GTGGTGCACA AGGTTCTGAA TGCATCAAAT GTCTTGGTGG ATGCAGAGGG CAATGTCAAG
    ATTACGGACT ACAGCATTTC AAAGCGCCTA GCAGACATTT GCAAGGAGGA TGTGTTTGAG
    CAGAGCCGAG TTAGTTTTAG TGACAGTGCC CTACCTTATA AAACGGGGAA GAAAGGGGAT
    GTTTGGTGTC TTGGCCTTCT GCTGCTCTTC CTCAGCCAAG GACAAGAATG CGGAGAGTAC
    CCTGTGACCA TCCCTAGCGA CTTACCAGCT GACTTCCAAG ATTTTCTAAA GAAGTGTGTT
    TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAATTGT TGAAACACAG CTTTATCAAT
    CCACAGCCGA AAATGTCTTT ATTGGAACAG AGTCCTGACG ATTCTGGAGG ACAAGATTAT
    GTTGAGACTG TTATTCCTAG TAAGCAGCTA CCCAGTGCAG CATTCTTCAG TGAGACACAG
    AGACAGTTTT CCCGGTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCC
    TTCGGAGCTG TCATCAAGGT GCAGAACAAG CTGGATGGCT GCTGCTACGC GGTGAAGCGC
    ATCCCCATCA ATCCGGCCAG TAGGCATTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTGC ACCACGAGAA CATAGTGCGC TACTATAACG CCTGGATAGA GAGATACGAG
    CGGCCCGTGG GCCCGGGGAT GCTGCCGCAG GACTCGGACT CATGGCCCCA GGCACCGGAT
    GGGCGAGCTT TGTCCCAGCC GTCCCCAGGC AACGACAGCC CCGACGGCGA CGGCGCCGGT
    AGCGTGGAGG CCGCTGCGCC ACCGCCCATC CTCAGCTCGG TGGAGTGGAG CACATCTGGC
    GAGCGTTCGG CCAGCGCCCG CTTCCCCTCC GCCCGCCCAG GCTCCAGCAG CGAGGAGGAC
    GACGACGACG ACGAGCACGA GGGCGTCTTC TCCCAGCCCT TCCTACCTGC TTCAGATTCT
    GACAGTGATG TCATCTTTGA AGATGAGAAC AGTAAGAGTC AGAATCAGGA AGAAGACTGC
    AATGGGAAGA ACAGTTGCCA TGAAAGTGAG CCATCGACAA CCACTGAAGC TGTGCACTAC
    CTGTATATCC AGATGGAGTA CTGTGAGAAG AGTACTTTGC GTGACACCAT TGACCAGGGG
    CTATATCAAG ATACCATCAG ACTCTGGAGG CTTTTTCGAG AGATTCTGGA TGGATTAGCT
    TATATCCATG AAAAAGGAAT GATTCACCGG GATTTGAAGC CTGTCAACAT CTTTTTGGAT
    TCTGATGACC ATGTAAAAAT AGGTGATTTT GGTTTAGCGA CAGACCATCT TGCCTTCACT
    GCTGATGGCA AGCAAGATGA TACAGCAGAT CACTTGATTA AATCAGACCC CTCAGGTCAT
    TTGACTGGGA TGGTTGGTAC TGCTTTATAC GTAAGCCCAG AGGTCCAAGG AAGCAGCAGA
    CCCACGTACA ACCAGAAAGT GGATCTCTTC AGCCTGGGAA TTATCTTCTT TGAGATGTCC
    TATCACCCCA TGGTCACGGC CTCAGAAAGG ATCTTTGTTC TCAACCAACT CAGAGATCCC
    ACTTCACCTA AGTTTCCAGA AGACTTCGAT GATGGAGAGC ATACAAAGCA GAAATCTGTC
    ATCTCCTGGC TGTTGAACCA TGATCCCGCC AAGCGGCCCA CAGCCACAGA GCTGCTCAAG
    AGTGAGCTGC TGCCCCCACC CCAGATGGAG GAGTCAGAGT TGCATGAGGT TCTGCACCAC
    ACCCTGGCCA ACGTGGATGG GAAGGCCTAC CGCACCATGA TGGCCCAGAT CTTCTCCCAG
    CGCGTCAGTC CAGCCATCGA TTACACCTAT GACAGCAACA TGCCGAAGGG CTTTTCAATC
    CGTACATCCA AGATACAGCA ACATGTGTGT GATACCATCG TCCGCATTTT TAAAAAACAC
    GGAGCTGTCC AGTTGTGTAC TCCACTACTG CTTCCCCAAA ATAGGCAAAT ATACGAGCAC
    AATGAAGCTG CCTTATTCAT GGACCACAGC GGGATGCTGG TGATGCTTCC CTTTGACCTA
    CGGGTTCCTT TTGCAAGATA TGTGGCAAGA AATAATATAT TGAATTTGAA ACGTTACTGC
    ATAGAACGTG TCTTCAGACC TCGCAAGTTA GACCGATTTC ATCCCAAAGA ACTTCTGGAA
    TGTGCATTTG ACATTGTTAC GTCTACCACC AACAGCTCTC TGCCCACTGC TGAAGTCATC
    TACACGATCT GTGAGATCAT CCAGGAGTTC CCGGCACTGC AGGAAAGAAA TTACAGTATT
    TACTTGAACC ACACCATGTT ATTGAAAGCA ATCTTCTTAC ACTGTGGGAT CCCAGAAGAT
    AAACTCAGTC AAGTCTATGT TATTCTATAT GATGCAGTGA CAGAAAAGCT GACAAGGAGA
    GAAGTGGAAG CCAAGTTTTG TAATCTGTCT CTGTCATCTA ATAGTCTGTG TCGACTCTAC
    AAATTCATTG AGCAGAAGGG AGATTTGCAA GATCTAACAC CAGCAATAAA TTCATTAATA
    AAACAGAAAA CAGGTGTTGC CCAACTGGTG AAGTATGGCT TGAAAGACTT AGAGGAGATC
    ATTGGGCTGT TGAAGAAACT CGGCATCAAG TTACAGGTCT TGATCAACTT GGGCTTGGTT
    TACAAAGTAC AGCAGCACAA CGGAGTCATC TTCCAGTTTG TGGCGGTCAT CAGACGGAGG
    CAAAGGGCTA TTTCAGAAAT CCTTGCAGCT GGAGGCAGAT ACGACCTGCT GATTCCCCAG
    TTTAGAGGAC CGCAGGCTCT GGGACCAGTC CCGACTGCTG TTGGGGTCAG CATAGCCATA
    GACAAGATAT CTGCAGCTAT CCTCAACATG GAAGAACCTG TTACAATAAG CTCTTGTGAC
    CTCCTGATCG TAAGTGTTGG CCAGATGTCC ATGTCCAGGG CCATCAACCT AACCCAGAAA
    CTTTGGTCAG CAGGAATCAC AGCACAGATC ATGTACGACT GGTCACAGTC CCAAGAAGAA
    CTCCAGGAAT ACTGCAGATA CCACGAAATC ACCTACGTGG CCCTCGTCTC AGATAAAGGA
    GGAAGCTATG TCAAGGTTAA GTCTTTTGAG AAGGAAAGGC AAACAGAGAA ACTTGTGCAG
    GAGTCTGATC TTATAGACCA CGTTTTGCAG AAACTCAGAA CTAAAGTCAG TGATGAAAGG
    ACTATCAGAG AAGCTTCTGA TAATCTCGCA GTGCAAAATC TGAAGGGGTC CTTTTCTAAT
    GCTTCAGGTT TGTTTGAAAT CCACGGAGCA GCAGTTGTCC CCATTGTGAG CGTGATAGCC
    CCAGAGAAGC TGTCAGCCAG CACGAGGAGG CGTTATGAAA CTCAGGTACA AACACGACTT
    CAGACCTCCC TTGCCAACTT ACATCAGAAA AGCAATGAAA TTGAAATTTT GGCTGTGGAT
    CTACCCAAAG AAACAATCTT ACAGTTTTTA TCGTTAGAGT GGGATGCTGA TGAACAGGCA
    TTTAACACAA CTGTGAAGCA GCTGCTGTCA CGCCTGCCAA AGCAGAGATA CCTCAAATTA
    GTCTGTGATG AAATTTATAA CATCAAAGTA GAGAAAAAGG TATCTGTGCT ATTCCTGTAT
    AGCTACAGAG ATGACTACTA CCGAATCTTA TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28637
    Clone ID Related Accession (Same CDS sequence) XM_005638229.2 , XM_005638229.3
    Accession Version XM_005638229.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product eukaryotic translation initiation factor 2-alpha kinase 4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638229.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
    CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC
    CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA
    CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT
    GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG
    CAACGTGAAA TCCTTCATGA GATTCAAAGA AGGAAAGAAG AGATAAAAGA AGAGAGAAAA
    AGAAAAGAAA TGGCTAAGCA GGAGCGTTTG GAAATTGCTA GTTTGTCAAA CCAAGATCAA
    AACTCTAGGA AAGACCCAGG AGGACACAGA ATATCTGCCA TTCTACATGG AGGCTCTCCT
    GACTTTGTAG GAAATGGTAA ACATCGGACC AACTCCTCTG GAAGGTCTAG GCGAGAACGT
    CAGTATTCTC TGTGTAGTAG TGAAGATTCT TCTGGCTCTT GTGAAGTCCT GTATTTCTCT
    ATGGGTAGCC CTGATCAGCT CATGGTTTAC AAAGGGAGGT GCATTGGCAG TGATGAGCAG
    CTTGGAAAAT TAGTTTATAA TGCTTTGGAA ACGGCCACTG GCAACTTTGT GTCATTACAT
    GAGTGGGTCC TTCGGTGGCA GAAAAAAATG GGCCCGTTCC TTACCAGTCA AGAGAAGGAG
    AAGATTGATA AGTGCAAAAA GCAGATTCAA GGGGCAGAAA CGGAATTCAA CTCACTGGTA
    AAATTGAGCC ATCCAAATGT AGTACGCTAC TTTGCAATGA ATCTCAAAGA GCAAGATGAC
    TCCATCATGG TGGACATTTT ATTGGAGCAC ATTAATGGGG TCTCCCTCTC TGCACATCTG
    AGCCACTCAG GCCCCATCCC TGTGTATCAA CTCCGCAAGT ACACAGCTCA GCTCTTGTCT
    GGCCTCGATT ATTTGCACAG CAATGACGTG GTGCACAAGG TTCTGAATGC ATCAAATGTC
    TTGGTGGATG CAGAGGGCAA TGTCAAGATT ACGGACTACA GCATTTCAAA GCGCCTAGCA
    GACATTTGCA AGGAGGATGT GTTTGAGCAG AGCCGAGTTA GTTTTAGTGA CAGTGCCCTA
    CCTTATAAAA CGGGGAAGAA AGGGGATGTT TGGTGTCTTG GCCTTCTGCT GCTCTTCCTC
    AGCCAAGGAC AAGAATGCGG AGAGTACCCT GTGACCATCC CTAGCGACTT ACCAGCTGAC
    TTCCAAGATT TTCTAAAGAA GTGTGTTTGC TTGGATGACA AGGAAAGATG GAGTCCCCAG
    CAATTGTTGA AACACAGCTT TATCAATCCA CAGCCGAAAA TGTCTTTATT GGAACAGAGT
    CCTGACGATT CTGGAGGACA AGATTATGTT GAGACTGTTA TTCCTAGTAA GCAGCTACCC
    AGTGCAGCAT TCTTCAGTGA GACACAGAGA CAGTTTTCCC GGTATTTCAT TGAGTTTGAA
    GAATTACAGC TTCTCGGCAA AGGAGCCTTC GGAGCTGTCA TCAAGGTGCA GAACAAGCTG
    GATGGCTGCT GCTACGCGGT GAAGCGCATC CCCATCAATC CGGCCAGTAG GCATTTCCGC
    AGGATCAAGG GCGAGGTGAC GCTGCTGTCG CGCCTGCACC ACGAGAACAT AGTGCGCTAC
    TATAACGCCT GGATAGAGAG ATACGAGCGG CCCGTGGGCC CGGGGATGCT GCCGCAGGAC
    TCGGACTCAT GGCCCCAGGC ACCGGATGGG CGAGCTTTGT CCCAGCCGTC CCCAGGCAAC
    GACAGCCCCG ACGGCGACGG CGCCGGTAGC GTGGAGGCCG CTGCGCCACC GCCCATCCTC
    AGCTCGGTGG AGTGGAGCAC ATCTGGCGAG CGTTCGGCCA GCGCCCGCTT CCCCTCCGCC
    CGCCCAGGCT CCAGCAGCGA GGAGGACGAC GACGACGACG AGCACGAGGG CGTCTTCTCC
    CAGCCCTTCC TACCTGCTTC AGATTCTGAC AGTGATGTCA TCTTTGAAGA TGAGAACAGT
    AAGAGTCAGA ATCAGGAAGA AGACTGCAAT GGGAAGAACA GTTGCCATGA AAGTGAGCCA
    TCGACAACCA CTGAAGCTGT GCACTACCTG TATATCCAGA TGGAGTACTG TGAGAAGAGT
    ACTTTGCGTG ACACCATTGA CCAGGGGCTA TATCAAGATA CCATCAGACT CTGGAGGCTT
    TTTCGAGAGA TTCTGGATGG ATTAGCTTAT ATCCATGAAA AAGGAATGAT TCACCGGGAT
    TTGAAGCCTG TCAACATCTT TTTGGATTCT GATGACCATG TAAAAATAGG TGATTTTGGT
    TTAGCGACAG ACCATCTTGC CTTCACTGCT GATGGCAAGC AAGATGATAC AGCAGATCAC
    TTGATTAAAT CAGACCCCTC AGGTCATTTG ACTGGGATGG TTGGTACTGC TTTATACGTA
    AGCCCAGAGG TCCAAGGAAG CAGCAGACCC ACGTACAACC AGAAAGTGGA TCTCTTCAGC
    CTGGGAATTA TCTTCTTTGA GATGTCCTAT CACCCCATGG TCACGGCCTC AGAAAGGATC
    TTTGTTCTCA ACCAACTCAG AGATCCCACT TCACCTAAGT TTCCAGAAGA CTTCGATGAT
    GGAGAGCATA CAAAGCAGAA ATCTGTCATC TCCTGGCTGT TGAACCATGA TCCCGCCAAG
    CGGCCCACAG CCACAGAGCT GCTCAAGAGT GAGCTGCTGC CCCCACCCCA GATGGAGGAG
    TCAGAGTTGC ATGAGGTTCT GCACCACACC CTGGCCAACG TGGATGGGAA GGCCTACCGC
    ACCATGATGG CCCAGATCTT CTCCCAGCGC GTCAGTCCAG CCATCGATTA CACCTATGAC
    AGCAACATGC CGAAGGGCTT TTCAATCCGT ACATCCAAGA TACAGCAACA TGTGTGTGAT
    ACCATCGTCC GCATTTTTAA AAAACACGGA GCTGTCCAGT TGTGTACTCC ACTACTGCTT
    CCCCAAAATA GGCAAATATA CGAGCACAAT GAAGCTGCCT TATTCATGGA CCACAGCGGG
    ATGCTGGTGA TGCTTCCCTT TGACCTACGG GTTCCTTTTG CAAGATATGT GGCAAGAAAT
    AATATATTGA ATTTGAAACG TTACTGCATA GAACGTGTCT TCAGACCTCG CAAGTTAGAC
    CGATTTCATC CCAAAGAACT TCTGGAATGT GCATTTGACA TTGTTACGTC TACCACCAAC
    AGCTCTCTGC CCACTGCTGA AGTCATCTAC ACGATCTGTG AGATCATCCA GGAGTTCCCG
    GCACTGCAGG AAAGAAATTA CAGTATTTAC TTGAACCACA CCATGTTATT GAAAGCAATC
    TTCTTACACT GTGGGATCCC AGAAGATAAA CTCAGTCAAG TCTATGTTAT TCTATATGAT
    GCAGTGACAG AAAAGCTGAC AAGGAGAGAA GTGGAAGCCA AGTTTTGTAA TCTGTCTCTG
    TCATCTAATA GTCTGTGTCG ACTCTACAAA TTCATTGAGC AGAAGGGAGA TTTGCAAGAT
    CTAACACCAG CAATAAATTC ATTAATAAAA CAGAAAACAG GTGTTGCCCA ACTGGTGAAG
    TATGGCTTGA AAGACTTAGA GGAGATCATT GGGCTGTTGA AGAAACTCGG CATCAAGTTA
    CAGGTCTTGA TCAACTTGGG CTTGGTTTAC AAAGTACAGC AGCACAACGG AGTCATCTTC
    CAGTTTGTGG CGGTCATCAG ACGGAGGCAA AGGGCTATTT CAGAAATCCT TGCAGCTGGA
    GGCAGATACG ACCTGCTGAT TCCCCAGTTT AGAGGACCGC AGGCTCTGGG ACCAGTCCCG
    ACTGCTGTTG GGGTCAGCAT AGCCATAGAC AAGATATCTG CAGCTATCCT CAACATGGAA
    GAACCTGTTA CAATAAGCTC TTGTGACCTC CTGATCGTAA GTGTTGGCCA GATGTCCATG
    TCCAGGGCCA TCAACCTAAC CCAGAAACTT TGGTCAGCAG GAATCACAGC ACAGATCATG
    TACGACTGGT CACAGTCCCA AGAAGAACTC CAGGAATACT GCAGATACCA CGAAATCACC
    TACGTGGCCC TCGTCTCAGA TAAAGGAGGA AGCTATGTCA AGGTTAAGTC TTTTGAGAAG
    GAAAGGCAAA CAGAGAAACT TGTGCAGGAG TCTGATCTTA TAGACCACGT TTTGCAGAAA
    CTCAGAACTA AAGTCAGTGA TGAAAGGACT ATCAGAGAAG CTTCTGATAA TCTCGCAGTG
    CAAAATCTGA AGGGGTCCTT TTCTAATGCT TCAGGTTTGT TTGAAATCCA CGGAGCAGCA
    GTTGTCCCCA TTGTGAGCGT GATAGCCCCA GAGAAGCTGT CAGCCAGCAC GAGGAGGCGT
    TATGAAACTC AGGTACAAAC ACGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC
    AATGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTTTTATCG
    TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC
    CTGCCAAAGC AGAGATACCT CAAATTAGTC TGTGATGAAA TTTATAACAT CAAAGTAGAG
    AAAAAGGTAT CTGTGCTATT CCTGTATAGC TACAGAGATG ACTACTACCG AATCTTATTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638286.1
    CDS91..4893
    Translation

    Target ORF information:

    RefSeq Version XM_005638229.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638229.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
    CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC
    CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA
    CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT
    GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG
    CAACGTGAAA TCCTTCATGA GATTCAAAGA AGGAAAGAAG AGATAAAAGA AGAGAGAAAA
    AGAAAAGAAA TGGCTAAGCA GGAGCGTTTG GAAATTGCTA GTTTGTCAAA CCAAGATCAA
    AACTCTAGGA AAGACCCAGG AGGACACAGA ATATCTGCCA TTCTACATGG AGGCTCTCCT
    GACTTTGTAG GAAATGGTAA ACATCGGACC AACTCCTCTG GAAGGTCTAG GCGAGAACGT
    CAGTATTCTC TGTGTAGTAG TGAAGATTCT TCTGGCTCTT GTGAAGTCCT GTATTTCTCT
    ATGGGTAGCC CTGATCAGCT CATGGTTTAC AAAGGGAGGT GCATTGGCAG TGATGAGCAG
    CTTGGAAAAT TAGTTTATAA TGCTTTGGAA ACGGCCACTG GCAACTTTGT GTCATTACAT
    GAGTGGGTCC TTCGGTGGCA GAAAAAAATG GGCCCGTTCC TTACCAGTCA AGAGAAGGAG
    AAGATTGATA AGTGCAAAAA GCAGATTCAA GGGGCAGAAA CGGAATTCAA CTCACTGGTA
    AAATTGAGCC ATCCAAATGT AGTACGCTAC TTTGCAATGA ATCTCAAAGA GCAAGATGAC
    TCCATCATGG TGGACATTTT ATTGGAGCAC ATTAATGGGG TCTCCCTCTC TGCACATCTG
    AGCCACTCAG GCCCCATCCC TGTGTATCAA CTCCGCAAGT ACACAGCTCA GCTCTTGTCT
    GGCCTCGATT ATTTGCACAG CAATGACGTG GTGCACAAGG TTCTGAATGC ATCAAATGTC
    TTGGTGGATG CAGAGGGCAA TGTCAAGATT ACGGACTACA GCATTTCAAA GCGCCTAGCA
    GACATTTGCA AGGAGGATGT GTTTGAGCAG AGCCGAGTTA GTTTTAGTGA CAGTGCCCTA
    CCTTATAAAA CGGGGAAGAA AGGGGATGTT TGGTGTCTTG GCCTTCTGCT GCTCTTCCTC
    AGCCAAGGAC AAGAATGCGG AGAGTACCCT GTGACCATCC CTAGCGACTT ACCAGCTGAC
    TTCCAAGATT TTCTAAAGAA GTGTGTTTGC TTGGATGACA AGGAAAGATG GAGTCCCCAG
    CAATTGTTGA AACACAGCTT TATCAATCCA CAGCCGAAAA TGTCTTTATT GGAACAGAGT
    CCTGACGATT CTGGAGGACA AGATTATGTT GAGACTGTTA TTCCTAGTAA GCAGCTACCC
    AGTGCAGCAT TCTTCAGTGA GACACAGAGA CAGTTTTCCC GGTATTTCAT TGAGTTTGAA
    GAATTACAGC TTCTCGGCAA AGGAGCCTTC GGAGCTGTCA TCAAGGTGCA GAACAAGCTG
    GATGGCTGCT GCTACGCGGT GAAGCGCATC CCCATCAATC CGGCCAGTAG GCATTTCCGC
    AGGATCAAGG GCGAGGTGAC GCTGCTGTCG CGCCTGCACC ACGAGAACAT AGTGCGCTAC
    TATAACGCCT GGATAGAGAG ATACGAGCGG CCCGTGGGCC CGGGGATGCT GCCGCAGGAC
    TCGGACTCAT GGCCCCAGGC ACCGGATGGG CGAGCTTTGT CCCAGCCGTC CCCAGGCAAC
    GACAGCCCCG ACGGCGACGG CGCCGGTAGC GTGGAGGCCG CTGCGCCACC GCCCATCCTC
    AGCTCGGTGG AGTGGAGCAC ATCTGGCGAG CGTTCGGCCA GCGCCCGCTT CCCCTCCGCC
    CGCCCAGGCT CCAGCAGCGA GGAGGACGAC GACGACGACG AGCACGAGGG CGTCTTCTCC
    CAGCCCTTCC TACCTGCTTC AGATTCTGAC AGTGATGTCA TCTTTGAAGA TGAGAACAGT
    AAGAGTCAGA ATCAGGAAGA AGACTGCAAT GGGAAGAACA GTTGCCATGA AAGTGAGCCA
    TCGACAACCA CTGAAGCTGT GCACTACCTG TATATCCAGA TGGAGTACTG TGAGAAGAGT
    ACTTTGCGTG ACACCATTGA CCAGGGGCTA TATCAAGATA CCATCAGACT CTGGAGGCTT
    TTTCGAGAGA TTCTGGATGG ATTAGCTTAT ATCCATGAAA AAGGAATGAT TCACCGGGAT
    TTGAAGCCTG TCAACATCTT TTTGGATTCT GATGACCATG TAAAAATAGG TGATTTTGGT
    TTAGCGACAG ACCATCTTGC CTTCACTGCT GATGGCAAGC AAGATGATAC AGCAGATCAC
    TTGATTAAAT CAGACCCCTC AGGTCATTTG ACTGGGATGG TTGGTACTGC TTTATACGTA
    AGCCCAGAGG TCCAAGGAAG CAGCAGACCC ACGTACAACC AGAAAGTGGA TCTCTTCAGC
    CTGGGAATTA TCTTCTTTGA GATGTCCTAT CACCCCATGG TCACGGCCTC AGAAAGGATC
    TTTGTTCTCA ACCAACTCAG AGATCCCACT TCACCTAAGT TTCCAGAAGA CTTCGATGAT
    GGAGAGCATA CAAAGCAGAA ATCTGTCATC TCCTGGCTGT TGAACCATGA TCCCGCCAAG
    CGGCCCACAG CCACAGAGCT GCTCAAGAGT GAGCTGCTGC CCCCACCCCA GATGGAGGAG
    TCAGAGTTGC ATGAGGTTCT GCACCACACC CTGGCCAACG TGGATGGGAA GGCCTACCGC
    ACCATGATGG CCCAGATCTT CTCCCAGCGC GTCAGTCCAG CCATCGATTA CACCTATGAC
    AGCAACATGC CGAAGGGCTT TTCAATCCGT ACATCCAAGA TACAGCAACA TGTGTGTGAT
    ACCATCGTCC GCATTTTTAA AAAACACGGA GCTGTCCAGT TGTGTACTCC ACTACTGCTT
    CCCCAAAATA GGCAAATATA CGAGCACAAT GAAGCTGCCT TATTCATGGA CCACAGCGGG
    ATGCTGGTGA TGCTTCCCTT TGACCTACGG GTTCCTTTTG CAAGATATGT GGCAAGAAAT
    AATATATTGA ATTTGAAACG TTACTGCATA GAACGTGTCT TCAGACCTCG CAAGTTAGAC
    CGATTTCATC CCAAAGAACT TCTGGAATGT GCATTTGACA TTGTTACGTC TACCACCAAC
    AGCTCTCTGC CCACTGCTGA AGTCATCTAC ACGATCTGTG AGATCATCCA GGAGTTCCCG
    GCACTGCAGG AAAGAAATTA CAGTATTTAC TTGAACCACA CCATGTTATT GAAAGCAATC
    TTCTTACACT GTGGGATCCC AGAAGATAAA CTCAGTCAAG TCTATGTTAT TCTATATGAT
    GCAGTGACAG AAAAGCTGAC AAGGAGAGAA GTGGAAGCCA AGTTTTGTAA TCTGTCTCTG
    TCATCTAATA GTCTGTGTCG ACTCTACAAA TTCATTGAGC AGAAGGGAGA TTTGCAAGAT
    CTAACACCAG CAATAAATTC ATTAATAAAA CAGAAAACAG GTGTTGCCCA ACTGGTGAAG
    TATGGCTTGA AAGACTTAGA GGAGATCATT GGGCTGTTGA AGAAACTCGG CATCAAGTTA
    CAGGTCTTGA TCAACTTGGG CTTGGTTTAC AAAGTACAGC AGCACAACGG AGTCATCTTC
    CAGTTTGTGG CGGTCATCAG ACGGAGGCAA AGGGCTATTT CAGAAATCCT TGCAGCTGGA
    GGCAGATACG ACCTGCTGAT TCCCCAGTTT AGAGGACCGC AGGCTCTGGG ACCAGTCCCG
    ACTGCTGTTG GGGTCAGCAT AGCCATAGAC AAGATATCTG CAGCTATCCT CAACATGGAA
    GAACCTGTTA CAATAAGCTC TTGTGACCTC CTGATCGTAA GTGTTGGCCA GATGTCCATG
    TCCAGGGCCA TCAACCTAAC CCAGAAACTT TGGTCAGCAG GAATCACAGC ACAGATCATG
    TACGACTGGT CACAGTCCCA AGAAGAACTC CAGGAATACT GCAGATACCA CGAAATCACC
    TACGTGGCCC TCGTCTCAGA TAAAGGAGGA AGCTATGTCA AGGTTAAGTC TTTTGAGAAG
    GAAAGGCAAA CAGAGAAACT TGTGCAGGAG TCTGATCTTA TAGACCACGT TTTGCAGAAA
    CTCAGAACTA AAGTCAGTGA TGAAAGGACT ATCAGAGAAG CTTCTGATAA TCTCGCAGTG
    CAAAATCTGA AGGGGTCCTT TTCTAATGCT TCAGGTTTGT TTGAAATCCA CGGAGCAGCA
    GTTGTCCCCA TTGTGAGCGT GATAGCCCCA GAGAAGCTGT CAGCCAGCAC GAGGAGGCGT
    TATGAAACTC AGGTACAAAC ACGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC
    AATGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTTTTATCG
    TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC
    CTGCCAAAGC AGAGATACCT CAAATTAGTC TGTGATGAAA TTTATAACAT CAAAGTAGAG
    AAAAAGGTAT CTGTGCTATT CCTGTATAGC TACAGAGATG ACTACTACCG AATCTTATTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28637
    Clone ID Related Accession (Same CDS sequence) XM_005638229.2 , XM_005638229.3
    Accession Version XM_005638229.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product eIF-2-alpha kinase GCN2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638229.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
    CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC
    CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA
    CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT
    GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG
    CAACGTGAAA TCCTTCATGA GATTCAAAGA AGGAAAGAAG AGATAAAAGA AGAGAGAAAA
    AGAAAAGAAA TGGCTAAGCA GGAGCGTTTG GAAATTGCTA GTTTGTCAAA CCAAGATCAA
    AACTCTAGGA AAGACCCAGG AGGACACAGA ATATCTGCCA TTCTACATGG AGGCTCTCCT
    GACTTTGTAG GAAATGGTAA ACATCGGACC AACTCCTCTG GAAGGTCTAG GCGAGAACGT
    CAGTATTCTC TGTGTAGTAG TGAAGATTCT TCTGGCTCTT GTGAAGTCCT GTATTTCTCT
    ATGGGTAGCC CTGATCAGCT CATGGTTTAC AAAGGGAGGT GCATTGGCAG TGATGAGCAG
    CTTGGAAAAT TAGTTTATAA TGCTTTGGAA ACGGCCACTG GCAACTTTGT GTCATTACAT
    GAGTGGGTCC TTCGGTGGCA GAAAAAAATG GGCCCGTTCC TTACCAGTCA AGAGAAGGAG
    AAGATTGATA AGTGCAAAAA GCAGATTCAA GGGGCAGAAA CGGAATTCAA CTCACTGGTA
    AAATTGAGCC ATCCAAATGT AGTACGCTAC TTTGCAATGA ATCTCAAAGA GCAAGATGAC
    TCCATCATGG TGGACATTTT ATTGGAGCAC ATTAATGGGG TCTCCCTCTC TGCACATCTG
    AGCCACTCAG GCCCCATCCC TGTGTATCAA CTCCGCAAGT ACACAGCTCA GCTCTTGTCT
    GGCCTCGATT ATTTGCACAG CAATGACGTG GTGCACAAGG TTCTGAATGC ATCAAATGTC
    TTGGTGGATG CAGAGGGCAA TGTCAAGATT ACGGACTACA GCATTTCAAA GCGCCTAGCA
    GACATTTGCA AGGAGGATGT GTTTGAGCAG AGCCGAGTTA GTTTTAGTGA CAGTGCCCTA
    CCTTATAAAA CGGGGAAGAA AGGGGATGTT TGGTGTCTTG GCCTTCTGCT GCTCTTCCTC
    AGCCAAGGAC AAGAATGCGG AGAGTACCCT GTGACCATCC CTAGCGACTT ACCAGCTGAC
    TTCCAAGATT TTCTAAAGAA GTGTGTTTGC TTGGATGACA AGGAAAGATG GAGTCCCCAG
    CAATTGTTGA AACACAGCTT TATCAATCCA CAGCCGAAAA TGTCTTTATT GGAACAGAGT
    CCTGACGATT CTGGAGGACA AGATTATGTT GAGACTGTTA TTCCTAGTAA GCAGCTACCC
    AGTGCAGCAT TCTTCAGTGA GACACAGAGA CAGTTTTCCC GGTATTTCAT TGAGTTTGAA
    GAATTACAGC TTCTCGGCAA AGGAGCCTTC GGAGCTGTCA TCAAGGTGCA GAACAAGCTG
    GATGGCTGCT GCTACGCGGT GAAGCGCATC CCCATCAATC CGGCCAGTAG GCATTTCCGC
    AGGATCAAGG GCGAGGTGAC GCTGCTGTCG CGCCTGCACC ACGAGAACAT AGTGCGCTAC
    TATAACGCCT GGATAGAGAG ATACGAGCGG CCCGTGGGCC CGGGGATGCT GCCGCAGGAC
    TCGGACTCAT GGCCCCAGGC ACCGGATGGG CGAGCTTTGT CCCAGCCGTC CCCAGGCAAC
    GACAGCCCCG ACGGCGACGG CGCCGGTAGC GTGGAGGCCG CTGCGCCACC GCCCATCCTC
    AGCTCGGTGG AGTGGAGCAC ATCTGGCGAG CGTTCGGCCA GCGCCCGCTT CCCCTCCGCC
    CGCCCAGGCT CCAGCAGCGA GGAGGACGAC GACGACGACG AGCACGAGGG CGTCTTCTCC
    CAGCCCTTCC TACCTGCTTC AGATTCTGAC AGTGATGTCA TCTTTGAAGA TGAGAACAGT
    AAGAGTCAGA ATCAGGAAGA AGACTGCAAT GGGAAGAACA GTTGCCATGA AAGTGAGCCA
    TCGACAACCA CTGAAGCTGT GCACTACCTG TATATCCAGA TGGAGTACTG TGAGAAGAGT
    ACTTTGCGTG ACACCATTGA CCAGGGGCTA TATCAAGATA CCATCAGACT CTGGAGGCTT
    TTTCGAGAGA TTCTGGATGG ATTAGCTTAT ATCCATGAAA AAGGAATGAT TCACCGGGAT
    TTGAAGCCTG TCAACATCTT TTTGGATTCT GATGACCATG TAAAAATAGG TGATTTTGGT
    TTAGCGACAG ACCATCTTGC CTTCACTGCT GATGGCAAGC AAGATGATAC AGCAGATCAC
    TTGATTAAAT CAGACCCCTC AGGTCATTTG ACTGGGATGG TTGGTACTGC TTTATACGTA
    AGCCCAGAGG TCCAAGGAAG CAGCAGACCC ACGTACAACC AGAAAGTGGA TCTCTTCAGC
    CTGGGAATTA TCTTCTTTGA GATGTCCTAT CACCCCATGG TCACGGCCTC AGAAAGGATC
    TTTGTTCTCA ACCAACTCAG AGATCCCACT TCACCTAAGT TTCCAGAAGA CTTCGATGAT
    GGAGAGCATA CAAAGCAGAA ATCTGTCATC TCCTGGCTGT TGAACCATGA TCCCGCCAAG
    CGGCCCACAG CCACAGAGCT GCTCAAGAGT GAGCTGCTGC CCCCACCCCA GATGGAGGAG
    TCAGAGTTGC ATGAGGTTCT GCACCACACC CTGGCCAACG TGGATGGGAA GGCCTACCGC
    ACCATGATGG CCCAGATCTT CTCCCAGCGC GTCAGTCCAG CCATCGATTA CACCTATGAC
    AGCAACATGC CGAAGGGCTT TTCAATCCGT ACATCCAAGA TACAGCAACA TGTGTGTGAT
    ACCATCGTCC GCATTTTTAA AAAACACGGA GCTGTCCAGT TGTGTACTCC ACTACTGCTT
    CCCCAAAATA GGCAAATATA CGAGCACAAT GAAGCTGCCT TATTCATGGA CCACAGCGGG
    ATGCTGGTGA TGCTTCCCTT TGACCTACGG GTTCCTTTTG CAAGATATGT GGCAAGAAAT
    AATATATTGA ATTTGAAACG TTACTGCATA GAACGTGTCT TCAGACCTCG CAAGTTAGAC
    CGATTTCATC CCAAAGAACT TCTGGAATGT GCATTTGACA TTGTTACGTC TACCACCAAC
    AGCTCTCTGC CCACTGCTGA AGTCATCTAC ACGATCTGTG AGATCATCCA GGAGTTCCCG
    GCACTGCAGG AAAGAAATTA CAGTATTTAC TTGAACCACA CCATGTTATT GAAAGCAATC
    TTCTTACACT GTGGGATCCC AGAAGATAAA CTCAGTCAAG TCTATGTTAT TCTATATGAT
    GCAGTGACAG AAAAGCTGAC AAGGAGAGAA GTGGAAGCCA AGTTTTGTAA TCTGTCTCTG
    TCATCTAATA GTCTGTGTCG ACTCTACAAA TTCATTGAGC AGAAGGGAGA TTTGCAAGAT
    CTAACACCAG CAATAAATTC ATTAATAAAA CAGAAAACAG GTGTTGCCCA ACTGGTGAAG
    TATGGCTTGA AAGACTTAGA GGAGATCATT GGGCTGTTGA AGAAACTCGG CATCAAGTTA
    CAGGTCTTGA TCAACTTGGG CTTGGTTTAC AAAGTACAGC AGCACAACGG AGTCATCTTC
    CAGTTTGTGG CGGTCATCAG ACGGAGGCAA AGGGCTATTT CAGAAATCCT TGCAGCTGGA
    GGCAGATACG ACCTGCTGAT TCCCCAGTTT AGAGGACCGC AGGCTCTGGG ACCAGTCCCG
    ACTGCTGTTG GGGTCAGCAT AGCCATAGAC AAGATATCTG CAGCTATCCT CAACATGGAA
    GAACCTGTTA CAATAAGCTC TTGTGACCTC CTGATCGTAA GTGTTGGCCA GATGTCCATG
    TCCAGGGCCA TCAACCTAAC CCAGAAACTT TGGTCAGCAG GAATCACAGC ACAGATCATG
    TACGACTGGT CACAGTCCCA AGAAGAACTC CAGGAATACT GCAGATACCA CGAAATCACC
    TACGTGGCCC TCGTCTCAGA TAAAGGAGGA AGCTATGTCA AGGTTAAGTC TTTTGAGAAG
    GAAAGGCAAA CAGAGAAACT TGTGCAGGAG TCTGATCTTA TAGACCACGT TTTGCAGAAA
    CTCAGAACTA AAGTCAGTGA TGAAAGGACT ATCAGAGAAG CTTCTGATAA TCTCGCAGTG
    CAAAATCTGA AGGGGTCCTT TTCTAATGCT TCAGGTTTGT TTGAAATCCA CGGAGCAGCA
    GTTGTCCCCA TTGTGAGCGT GATAGCCCCA GAGAAGCTGT CAGCCAGCAC GAGGAGGCGT
    TATGAAACTC AGGTACAAAC ACGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC
    AATGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTTTTATCG
    TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC
    CTGCCAAAGC AGAGATACCT CAAATTAGTC TGTGATGAAA TTTATAACAT CAAAGTAGAG
    AAAAAGGTAT CTGTGCTATT CCTGTATAGC TACAGAGATG ACTACTACCG AATCTTATTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638286.1
    CDS57..4859
    Translation

    Target ORF information:

    RefSeq Version XM_005638229.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638229.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
    CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC
    CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA
    CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT
    GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG
    CAACGTGAAA TCCTTCATGA GATTCAAAGA AGGAAAGAAG AGATAAAAGA AGAGAGAAAA
    AGAAAAGAAA TGGCTAAGCA GGAGCGTTTG GAAATTGCTA GTTTGTCAAA CCAAGATCAA
    AACTCTAGGA AAGACCCAGG AGGACACAGA ATATCTGCCA TTCTACATGG AGGCTCTCCT
    GACTTTGTAG GAAATGGTAA ACATCGGACC AACTCCTCTG GAAGGTCTAG GCGAGAACGT
    CAGTATTCTC TGTGTAGTAG TGAAGATTCT TCTGGCTCTT GTGAAGTCCT GTATTTCTCT
    ATGGGTAGCC CTGATCAGCT CATGGTTTAC AAAGGGAGGT GCATTGGCAG TGATGAGCAG
    CTTGGAAAAT TAGTTTATAA TGCTTTGGAA ACGGCCACTG GCAACTTTGT GTCATTACAT
    GAGTGGGTCC TTCGGTGGCA GAAAAAAATG GGCCCGTTCC TTACCAGTCA AGAGAAGGAG
    AAGATTGATA AGTGCAAAAA GCAGATTCAA GGGGCAGAAA CGGAATTCAA CTCACTGGTA
    AAATTGAGCC ATCCAAATGT AGTACGCTAC TTTGCAATGA ATCTCAAAGA GCAAGATGAC
    TCCATCATGG TGGACATTTT ATTGGAGCAC ATTAATGGGG TCTCCCTCTC TGCACATCTG
    AGCCACTCAG GCCCCATCCC TGTGTATCAA CTCCGCAAGT ACACAGCTCA GCTCTTGTCT
    GGCCTCGATT ATTTGCACAG CAATGACGTG GTGCACAAGG TTCTGAATGC ATCAAATGTC
    TTGGTGGATG CAGAGGGCAA TGTCAAGATT ACGGACTACA GCATTTCAAA GCGCCTAGCA
    GACATTTGCA AGGAGGATGT GTTTGAGCAG AGCCGAGTTA GTTTTAGTGA CAGTGCCCTA
    CCTTATAAAA CGGGGAAGAA AGGGGATGTT TGGTGTCTTG GCCTTCTGCT GCTCTTCCTC
    AGCCAAGGAC AAGAATGCGG AGAGTACCCT GTGACCATCC CTAGCGACTT ACCAGCTGAC
    TTCCAAGATT TTCTAAAGAA GTGTGTTTGC TTGGATGACA AGGAAAGATG GAGTCCCCAG
    CAATTGTTGA AACACAGCTT TATCAATCCA CAGCCGAAAA TGTCTTTATT GGAACAGAGT
    CCTGACGATT CTGGAGGACA AGATTATGTT GAGACTGTTA TTCCTAGTAA GCAGCTACCC
    AGTGCAGCAT TCTTCAGTGA GACACAGAGA CAGTTTTCCC GGTATTTCAT TGAGTTTGAA
    GAATTACAGC TTCTCGGCAA AGGAGCCTTC GGAGCTGTCA TCAAGGTGCA GAACAAGCTG
    GATGGCTGCT GCTACGCGGT GAAGCGCATC CCCATCAATC CGGCCAGTAG GCATTTCCGC
    AGGATCAAGG GCGAGGTGAC GCTGCTGTCG CGCCTGCACC ACGAGAACAT AGTGCGCTAC
    TATAACGCCT GGATAGAGAG ATACGAGCGG CCCGTGGGCC CGGGGATGCT GCCGCAGGAC
    TCGGACTCAT GGCCCCAGGC ACCGGATGGG CGAGCTTTGT CCCAGCCGTC CCCAGGCAAC
    GACAGCCCCG ACGGCGACGG CGCCGGTAGC GTGGAGGCCG CTGCGCCACC GCCCATCCTC
    AGCTCGGTGG AGTGGAGCAC ATCTGGCGAG CGTTCGGCCA GCGCCCGCTT CCCCTCCGCC
    CGCCCAGGCT CCAGCAGCGA GGAGGACGAC GACGACGACG AGCACGAGGG CGTCTTCTCC
    CAGCCCTTCC TACCTGCTTC AGATTCTGAC AGTGATGTCA TCTTTGAAGA TGAGAACAGT
    AAGAGTCAGA ATCAGGAAGA AGACTGCAAT GGGAAGAACA GTTGCCATGA AAGTGAGCCA
    TCGACAACCA CTGAAGCTGT GCACTACCTG TATATCCAGA TGGAGTACTG TGAGAAGAGT
    ACTTTGCGTG ACACCATTGA CCAGGGGCTA TATCAAGATA CCATCAGACT CTGGAGGCTT
    TTTCGAGAGA TTCTGGATGG ATTAGCTTAT ATCCATGAAA AAGGAATGAT TCACCGGGAT
    TTGAAGCCTG TCAACATCTT TTTGGATTCT GATGACCATG TAAAAATAGG TGATTTTGGT
    TTAGCGACAG ACCATCTTGC CTTCACTGCT GATGGCAAGC AAGATGATAC AGCAGATCAC
    TTGATTAAAT CAGACCCCTC AGGTCATTTG ACTGGGATGG TTGGTACTGC TTTATACGTA
    AGCCCAGAGG TCCAAGGAAG CAGCAGACCC ACGTACAACC AGAAAGTGGA TCTCTTCAGC
    CTGGGAATTA TCTTCTTTGA GATGTCCTAT CACCCCATGG TCACGGCCTC AGAAAGGATC
    TTTGTTCTCA ACCAACTCAG AGATCCCACT TCACCTAAGT TTCCAGAAGA CTTCGATGAT
    GGAGAGCATA CAAAGCAGAA ATCTGTCATC TCCTGGCTGT TGAACCATGA TCCCGCCAAG
    CGGCCCACAG CCACAGAGCT GCTCAAGAGT GAGCTGCTGC CCCCACCCCA GATGGAGGAG
    TCAGAGTTGC ATGAGGTTCT GCACCACACC CTGGCCAACG TGGATGGGAA GGCCTACCGC
    ACCATGATGG CCCAGATCTT CTCCCAGCGC GTCAGTCCAG CCATCGATTA CACCTATGAC
    AGCAACATGC CGAAGGGCTT TTCAATCCGT ACATCCAAGA TACAGCAACA TGTGTGTGAT
    ACCATCGTCC GCATTTTTAA AAAACACGGA GCTGTCCAGT TGTGTACTCC ACTACTGCTT
    CCCCAAAATA GGCAAATATA CGAGCACAAT GAAGCTGCCT TATTCATGGA CCACAGCGGG
    ATGCTGGTGA TGCTTCCCTT TGACCTACGG GTTCCTTTTG CAAGATATGT GGCAAGAAAT
    AATATATTGA ATTTGAAACG TTACTGCATA GAACGTGTCT TCAGACCTCG CAAGTTAGAC
    CGATTTCATC CCAAAGAACT TCTGGAATGT GCATTTGACA TTGTTACGTC TACCACCAAC
    AGCTCTCTGC CCACTGCTGA AGTCATCTAC ACGATCTGTG AGATCATCCA GGAGTTCCCG
    GCACTGCAGG AAAGAAATTA CAGTATTTAC TTGAACCACA CCATGTTATT GAAAGCAATC
    TTCTTACACT GTGGGATCCC AGAAGATAAA CTCAGTCAAG TCTATGTTAT TCTATATGAT
    GCAGTGACAG AAAAGCTGAC AAGGAGAGAA GTGGAAGCCA AGTTTTGTAA TCTGTCTCTG
    TCATCTAATA GTCTGTGTCG ACTCTACAAA TTCATTGAGC AGAAGGGAGA TTTGCAAGAT
    CTAACACCAG CAATAAATTC ATTAATAAAA CAGAAAACAG GTGTTGCCCA ACTGGTGAAG
    TATGGCTTGA AAGACTTAGA GGAGATCATT GGGCTGTTGA AGAAACTCGG CATCAAGTTA
    CAGGTCTTGA TCAACTTGGG CTTGGTTTAC AAAGTACAGC AGCACAACGG AGTCATCTTC
    CAGTTTGTGG CGGTCATCAG ACGGAGGCAA AGGGCTATTT CAGAAATCCT TGCAGCTGGA
    GGCAGATACG ACCTGCTGAT TCCCCAGTTT AGAGGACCGC AGGCTCTGGG ACCAGTCCCG
    ACTGCTGTTG GGGTCAGCAT AGCCATAGAC AAGATATCTG CAGCTATCCT CAACATGGAA
    GAACCTGTTA CAATAAGCTC TTGTGACCTC CTGATCGTAA GTGTTGGCCA GATGTCCATG
    TCCAGGGCCA TCAACCTAAC CCAGAAACTT TGGTCAGCAG GAATCACAGC ACAGATCATG
    TACGACTGGT CACAGTCCCA AGAAGAACTC CAGGAATACT GCAGATACCA CGAAATCACC
    TACGTGGCCC TCGTCTCAGA TAAAGGAGGA AGCTATGTCA AGGTTAAGTC TTTTGAGAAG
    GAAAGGCAAA CAGAGAAACT TGTGCAGGAG TCTGATCTTA TAGACCACGT TTTGCAGAAA
    CTCAGAACTA AAGTCAGTGA TGAAAGGACT ATCAGAGAAG CTTCTGATAA TCTCGCAGTG
    CAAAATCTGA AGGGGTCCTT TTCTAATGCT TCAGGTTTGT TTGAAATCCA CGGAGCAGCA
    GTTGTCCCCA TTGTGAGCGT GATAGCCCCA GAGAAGCTGT CAGCCAGCAC GAGGAGGCGT
    TATGAAACTC AGGTACAAAC ACGACTTCAG ACCTCCCTTG CCAACTTACA TCAGAAAAGC
    AATGAAATTG AAATTTTGGC TGTGGATCTA CCCAAAGAAA CAATCTTACA GTTTTTATCG
    TTAGAGTGGG ATGCTGATGA ACAGGCATTT AACACAACTG TGAAGCAGCT GCTGTCACGC
    CTGCCAAAGC AGAGATACCT CAAATTAGTC TGTGATGAAA TTTATAACAT CAAAGTAGAG
    AAAAAGGTAT CTGTGCTATT CCTGTATAGC TACAGAGATG ACTACTACCG AATCTTATTT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28638
    Clone ID Related Accession (Same CDS sequence) XM_005638230.2
    Accession Version XM_005638230.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product eukaryotic translation initiation factor 2-alpha kinase 4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638230.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
    CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC
    CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA
    CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT
    GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG
    GTGATGATAT TTGAACTGGC CGACCATGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC
    CCTCCCAAGT CTTTCCATGA GGAAATGCTA GAAAGGCGGG CTCAGGAGGA GCAGCGGAGG
    CTGTTGGAGG CCAAGCAGAA GGTAGAGCAG GAGCAACGTG AAATCCTTCA TGAGATTCAA
    AGAAGGAAAG AAGAGATAAA AGAAGAGAGA AAAAGAAAAG AAATGGCTAA GCAGGAGCGT
    TTGGAAATTG CTAGTTTGTC AAACCAAGAT CAAAACTCTA GGAAAGACCC AGGAGGACAC
    AGAATATCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAGGCGAGAA CGTCAGTATT CTCTGTGTAG TAGTGAAGAT
    TCTTCTGGCT CTTGTGAAGT CCTGTATTTC TCTATGGGTA GCCCTGATCA GCTCATGGTT
    TACAAAGGGA GGTGCATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA TAATGCTTTG
    GAAACGGCCA CTGGCAACTT TGTGTCATTA CATGAGTGGG TCCTTCGGTG GCAGAAAAAA
    ATGGGCCCGT TCCTTACCAG TCAAGAGAAG GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACGGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC
    TACTTTGCAA TGAATCTCAA AGAGCAAGAT GACTCCATCA TGGTGGACAT TTTATTGGAG
    CACATTAATG GGGTCTCCCT CTCTGCACAT CTGAGCCACT CAGGCCCCAT CCCTGTGTAT
    CAACTCCGCA AGTACACAGC TCAGCTCTTG TCTGGCCTCG ATTATTTGCA CAGCAATGAC
    GTGGTGCACA AGGTTCTGAA TGCATCAAAT GTCTTGGTGG ATGCAGAGGG CAATGTCAAG
    ATTACGGACT ACAGCATTTC AAAGCGCCTA GCAGACATTT GCAAGGAGGA TGTGTTTGAG
    CAGAGCCGAG TTAGTTTTAG TGACAGTGCC CTACCTTATA AAACGGGGAA GAAAGGGGAT
    GTTTGGTGTC TTGGCCTTCT GCTGCTCTTC CTCAGCCAAG GACAAGAATG CGGAGAGTAC
    CCTGTGACCA TCCCTAGCGA CTTACCAGCT GACTTCCAAG ATTTTCTAAA GAAGTGTGTT
    TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAATTGT TGAAACACAG CTTTATCAAT
    CCACAGCCGA AAATGTCTTT ATTGGAACAG AGTCCTGACG ATTCTGGAGG ACAAGATTAT
    GTTGAGACTG TTATTCCTAG TAAGCAGCTA CCCAGTGCAG CATTCTTCAG TGAGACACAG
    AGACAGTTTT CCCGGTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCC
    TTCGGAGCTG TCATCAAGGT GCAGAACAAG CTGGATGGCT GCTGCTACGC GGTGAAGCGC
    ATCCCCATCA ATCCGGCCAG TAGGCATTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTGC ACCACGAGAA CATAGTGCGC TACTATAACG CCTGGATAGA GAGATACGAG
    CGGCCCGTGG GCCCGGGGAT GCTGCCGCAG GACTCGGACT CATGGCCCCA GGCACCGGAT
    GGGCGAGCTT TGTCCCAGCC GTCCCCAGGC AACGACAGCC CCGACGGCGA CGGCGCCGGT
    AGCGTGGAGG CCGCTGCGCC ACCGCCCATC CTCAGCTCGG TGGAGTGGAG CACATCTGGC
    GAGCGTTCGG CCAGCGCCCG CTTCCCCTCC GCCCGCCCAG GCTCCAGCAG CGAGGAGGAC
    GACGACGACG ACGAGCACGA GGGCGTCTTC TCCCAGCCCT TCCTACCTGC TTCAGATTCT
    GACAGTGATG TCATCTTTGA AGATGAGAAC AGTAAGAGTC AGAATCAGGA AGAAGACTGC
    AATGGGAAGA ACAGTTGCCA TGAAAGTGAG CCATCGACAA CCACTGAAGC TGTGCACTAC
    CTGTATATCC AGATGGAGTA CTGTGAGAAG AGTACTTTGC GTGACACCAT TGACCAGGGG
    CTATATCAAG ATACCATCAG ACTCTGGAGG CTTTTTCGAG AGATTCTGGA TGGATTAGCT
    TATATCCATG AAAAAGGAAT GATTCACCGG GATTTGAAGC CTGTCAACAT CTTTTTGGAT
    TCTGATGACC ATGTAAAAAT AGGTGATTTT GGTTTAGCGA CAGACCATCT TGCCTTCACT
    GCTGATGGCA AGCAAGATGA TACAGCAGAT CACTTGATTA AATCAGACCC CTCAGGTCAT
    TTGACTGGGA TGGTTGGTAC TGCTTTATAC GTAAGCCCAG AGGTCCAAGG AAGCAGCAGA
    CCCACGTACA ACCAGAAAGT GGATCTCTTC AGCCTGGGAA TTATCTTCTT TGAGATGTCC
    TATCACCCCA TGGTCACGGC CTCAGAAAGG ATCTTTGTTC TCAACCAACT CAGAGATCCC
    ACTTCACCTA AGTTTCCAGA AGACTTCGAT GATGGAGAGC ATACAAAGCA GAAATCTGTC
    ATCTCCTGGC TGTTGAACCA TGATCCCGCC AAGCGGCCCA CAGCCACAGA GCTGCTCAAG
    AGTGAGCTGC TGCCCCCACC CCAGATGGAG GAGTCAGAGT TGCATGAGGT TCTGCACCAC
    ACCCTGGCCA ACGTGGATGG GAAGGCCTAC CGCACCATGA TGGCCCAGAT CTTCTCCCAG
    CGCGTCAGTC CAGCCATCGA TTACACCTAT GACAGCAACA TGCCGAAGGG CTTTTCAATC
    CGTACATCCA AGATACAGCA ACATGTGTGT GATACCATCG TCCGCATTTT TAAAAAACAC
    GGAGCTGTCC AGTTGTGTAC TCCACTACTG CTTCCCCAAA ATAGGCAAAT ATACGAGCAC
    AATGAAGCTG CCTTATTCAT GGACCACAGC GGGATGCTGG TGATGCTTCC CTTTGACCTA
    CGGGTTCCTT TTGCAAGATA TGTGGCAAGA AATAATATAT TGAATTTGAA ACGTTACTGC
    ATAGAACGTG TCTTCAGACC TCGCAAGTTA GACCGATTTC ATCCCAAAGA ACTTCTGGAA
    TGTGCATTTG ACATTGTTAC GTCTACCACC AACAGCTCTC TGCCCACTGC TGAAGTCATC
    TACACGATCT GTGAGATCAT CCAGGAGTTC CCGGCACTGC AGGAAAGAAA TTACAGTATT
    TACTTGAACC ACACCATGTT ATTGAAAGCA ATCTTCTTAC ACTGTGGGAT CCCAGAAGAT
    AAACTCAGTC AAGTCTATGT TATTCTATAT GATGCAGTGA CAGAAAAGCT GACAAGGAGA
    GAAGTGGAAG CCAAGTTTTG TAATCTGTCT CTGTCATCTA ATAGTCTGTG TCGACTCTAC
    AAATTCATTG AGCAGAAGGG AGATTTGCAA GATCTAACAC CAGCAATAAA TTCATTAATA
    AAACAGAAAA CAGGTGTTGC CCAACTGGTG AAGTATGGCT TGAAAGACTT AGAGGAGATC
    ATTGGGCTGT TGAAGAAACT CGGCATCAAG TTACAGGTCT TGATCAACTT GGGCTTGGTT
    TACAAAGTAC AGCAGCACAA CGGAGTCATC TTCCAGTTTG TGGCGGTCAT CAGACGGAGG
    CAAAGGGCTA TTTCAGAAAT CCTTGCAGCT GGAGGCAGAT ACGACCTGCT GATTCCCCAG
    TTTAGAGGAC CGCAGGCTCT GGGACCAGTC CCGACTGCTG TTGGGGTCAG CATAGCCATA
    GACAAGATAT CTGCAGCTAT CCTCAACATG GAAGAACCTG TTACAATAAG CTCTTGTGAC
    CTCCTGATCG TAAGTGTTGG CCAGATGTCC ATGTCCAGGG CCATCAACCT AACCCAGAAA
    CTTTGGTCAG CAGGAATCAC AGCACAGATC ATGTACGACT GGTCACAGTC CCAAGAAGAA
    CTCCAGGAAT ACTGCAGATA CCACGAAATC ACCTACGTGG CCCTCGTCTC AGATAAAGGA
    GGAAGCTATG TCAAGGTTAA GTCTTTTGAG AAGGAAAGGC AAACAGAGAA ACTTGTGCAG
    GAGTCTGATC TTATAGACCA CGTTTTGCAG AAACTCAGAA CTAAAGTCAG TGATGAAAGG
    ACTATCAGGT TTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638287.1
    CDS92..4609
    Translation

    Target ORF information:

    RefSeq Version XM_005638230.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638230.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
    CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC
    CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA
    CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT
    GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG
    GTGATGATAT TTGAACTGGC CGACCATGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC
    CCTCCCAAGT CTTTCCATGA GGAAATGCTA GAAAGGCGGG CTCAGGAGGA GCAGCGGAGG
    CTGTTGGAGG CCAAGCAGAA GGTAGAGCAG GAGCAACGTG AAATCCTTCA TGAGATTCAA
    AGAAGGAAAG AAGAGATAAA AGAAGAGAGA AAAAGAAAAG AAATGGCTAA GCAGGAGCGT
    TTGGAAATTG CTAGTTTGTC AAACCAAGAT CAAAACTCTA GGAAAGACCC AGGAGGACAC
    AGAATATCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAGGCGAGAA CGTCAGTATT CTCTGTGTAG TAGTGAAGAT
    TCTTCTGGCT CTTGTGAAGT CCTGTATTTC TCTATGGGTA GCCCTGATCA GCTCATGGTT
    TACAAAGGGA GGTGCATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA TAATGCTTTG
    GAAACGGCCA CTGGCAACTT TGTGTCATTA CATGAGTGGG TCCTTCGGTG GCAGAAAAAA
    ATGGGCCCGT TCCTTACCAG TCAAGAGAAG GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACGGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC
    TACTTTGCAA TGAATCTCAA AGAGCAAGAT GACTCCATCA TGGTGGACAT TTTATTGGAG
    CACATTAATG GGGTCTCCCT CTCTGCACAT CTGAGCCACT CAGGCCCCAT CCCTGTGTAT
    CAACTCCGCA AGTACACAGC TCAGCTCTTG TCTGGCCTCG ATTATTTGCA CAGCAATGAC
    GTGGTGCACA AGGTTCTGAA TGCATCAAAT GTCTTGGTGG ATGCAGAGGG CAATGTCAAG
    ATTACGGACT ACAGCATTTC AAAGCGCCTA GCAGACATTT GCAAGGAGGA TGTGTTTGAG
    CAGAGCCGAG TTAGTTTTAG TGACAGTGCC CTACCTTATA AAACGGGGAA GAAAGGGGAT
    GTTTGGTGTC TTGGCCTTCT GCTGCTCTTC CTCAGCCAAG GACAAGAATG CGGAGAGTAC
    CCTGTGACCA TCCCTAGCGA CTTACCAGCT GACTTCCAAG ATTTTCTAAA GAAGTGTGTT
    TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAATTGT TGAAACACAG CTTTATCAAT
    CCACAGCCGA AAATGTCTTT ATTGGAACAG AGTCCTGACG ATTCTGGAGG ACAAGATTAT
    GTTGAGACTG TTATTCCTAG TAAGCAGCTA CCCAGTGCAG CATTCTTCAG TGAGACACAG
    AGACAGTTTT CCCGGTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCC
    TTCGGAGCTG TCATCAAGGT GCAGAACAAG CTGGATGGCT GCTGCTACGC GGTGAAGCGC
    ATCCCCATCA ATCCGGCCAG TAGGCATTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTGC ACCACGAGAA CATAGTGCGC TACTATAACG CCTGGATAGA GAGATACGAG
    CGGCCCGTGG GCCCGGGGAT GCTGCCGCAG GACTCGGACT CATGGCCCCA GGCACCGGAT
    GGGCGAGCTT TGTCCCAGCC GTCCCCAGGC AACGACAGCC CCGACGGCGA CGGCGCCGGT
    AGCGTGGAGG CCGCTGCGCC ACCGCCCATC CTCAGCTCGG TGGAGTGGAG CACATCTGGC
    GAGCGTTCGG CCAGCGCCCG CTTCCCCTCC GCCCGCCCAG GCTCCAGCAG CGAGGAGGAC
    GACGACGACG ACGAGCACGA GGGCGTCTTC TCCCAGCCCT TCCTACCTGC TTCAGATTCT
    GACAGTGATG TCATCTTTGA AGATGAGAAC AGTAAGAGTC AGAATCAGGA AGAAGACTGC
    AATGGGAAGA ACAGTTGCCA TGAAAGTGAG CCATCGACAA CCACTGAAGC TGTGCACTAC
    CTGTATATCC AGATGGAGTA CTGTGAGAAG AGTACTTTGC GTGACACCAT TGACCAGGGG
    CTATATCAAG ATACCATCAG ACTCTGGAGG CTTTTTCGAG AGATTCTGGA TGGATTAGCT
    TATATCCATG AAAAAGGAAT GATTCACCGG GATTTGAAGC CTGTCAACAT CTTTTTGGAT
    TCTGATGACC ATGTAAAAAT AGGTGATTTT GGTTTAGCGA CAGACCATCT TGCCTTCACT
    GCTGATGGCA AGCAAGATGA TACAGCAGAT CACTTGATTA AATCAGACCC CTCAGGTCAT
    TTGACTGGGA TGGTTGGTAC TGCTTTATAC GTAAGCCCAG AGGTCCAAGG AAGCAGCAGA
    CCCACGTACA ACCAGAAAGT GGATCTCTTC AGCCTGGGAA TTATCTTCTT TGAGATGTCC
    TATCACCCCA TGGTCACGGC CTCAGAAAGG ATCTTTGTTC TCAACCAACT CAGAGATCCC
    ACTTCACCTA AGTTTCCAGA AGACTTCGAT GATGGAGAGC ATACAAAGCA GAAATCTGTC
    ATCTCCTGGC TGTTGAACCA TGATCCCGCC AAGCGGCCCA CAGCCACAGA GCTGCTCAAG
    AGTGAGCTGC TGCCCCCACC CCAGATGGAG GAGTCAGAGT TGCATGAGGT TCTGCACCAC
    ACCCTGGCCA ACGTGGATGG GAAGGCCTAC CGCACCATGA TGGCCCAGAT CTTCTCCCAG
    CGCGTCAGTC CAGCCATCGA TTACACCTAT GACAGCAACA TGCCGAAGGG CTTTTCAATC
    CGTACATCCA AGATACAGCA ACATGTGTGT GATACCATCG TCCGCATTTT TAAAAAACAC
    GGAGCTGTCC AGTTGTGTAC TCCACTACTG CTTCCCCAAA ATAGGCAAAT ATACGAGCAC
    AATGAAGCTG CCTTATTCAT GGACCACAGC GGGATGCTGG TGATGCTTCC CTTTGACCTA
    CGGGTTCCTT TTGCAAGATA TGTGGCAAGA AATAATATAT TGAATTTGAA ACGTTACTGC
    ATAGAACGTG TCTTCAGACC TCGCAAGTTA GACCGATTTC ATCCCAAAGA ACTTCTGGAA
    TGTGCATTTG ACATTGTTAC GTCTACCACC AACAGCTCTC TGCCCACTGC TGAAGTCATC
    TACACGATCT GTGAGATCAT CCAGGAGTTC CCGGCACTGC AGGAAAGAAA TTACAGTATT
    TACTTGAACC ACACCATGTT ATTGAAAGCA ATCTTCTTAC ACTGTGGGAT CCCAGAAGAT
    AAACTCAGTC AAGTCTATGT TATTCTATAT GATGCAGTGA CAGAAAAGCT GACAAGGAGA
    GAAGTGGAAG CCAAGTTTTG TAATCTGTCT CTGTCATCTA ATAGTCTGTG TCGACTCTAC
    AAATTCATTG AGCAGAAGGG AGATTTGCAA GATCTAACAC CAGCAATAAA TTCATTAATA
    AAACAGAAAA CAGGTGTTGC CCAACTGGTG AAGTATGGCT TGAAAGACTT AGAGGAGATC
    ATTGGGCTGT TGAAGAAACT CGGCATCAAG TTACAGGTCT TGATCAACTT GGGCTTGGTT
    TACAAAGTAC AGCAGCACAA CGGAGTCATC TTCCAGTTTG TGGCGGTCAT CAGACGGAGG
    CAAAGGGCTA TTTCAGAAAT CCTTGCAGCT GGAGGCAGAT ACGACCTGCT GATTCCCCAG
    TTTAGAGGAC CGCAGGCTCT GGGACCAGTC CCGACTGCTG TTGGGGTCAG CATAGCCATA
    GACAAGATAT CTGCAGCTAT CCTCAACATG GAAGAACCTG TTACAATAAG CTCTTGTGAC
    CTCCTGATCG TAAGTGTTGG CCAGATGTCC ATGTCCAGGG CCATCAACCT AACCCAGAAA
    CTTTGGTCAG CAGGAATCAC AGCACAGATC ATGTACGACT GGTCACAGTC CCAAGAAGAA
    CTCCAGGAAT ACTGCAGATA CCACGAAATC ACCTACGTGG CCCTCGTCTC AGATAAAGGA
    GGAAGCTATG TCAAGGTTAA GTCTTTTGAG AAGGAAAGGC AAACAGAGAA ACTTGTGCAG
    GAGTCTGATC TTATAGACCA CGTTTTGCAG AAACTCAGAA CTAAAGTCAG TGATGAAAGG
    ACTATCAGGT TTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28639
    Clone ID Related Accession (Same CDS sequence) XM_005638231.2 , XM_005638231.3
    Accession Version XM_005638231.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product eukaryotic translation initiation factor 2-alpha kinase 4 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638231.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
    CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC
    CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA
    CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT
    GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG
    GTGATGATAT TTGAACTGGC CGACCATGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC
    CCTCCCAAGT CTTTCCATGA GGAAATGCTA GAAAGGCGGG CTCAGGAGGA GCAGCGGAGG
    CTGTTGGAGG CCAAGCAGAA GGTAGAGCAG GAGCAACGTG AAATCCTTCA TGAGATTCAA
    AGAAGGAAAG AAGAGATAAA AGAAGAGAGA AAAAGAAAAG AAATGGCTAA GCAGGAGCGT
    TTGGAAATTG CTAGTTTGTC AAACCAAGAT CAAAACTCTA GGAAAGACCC AGGAGGACAC
    AGAATATCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAGGCGAGAA CGTCAGTATT CTCTGTGTAG TAGTGAAGAT
    TCTTCTGGCT CTTGTGAAGT CCTGTATTTC TCTATGGGTA GCCCTGATCA GCTCATGGTT
    TACAAAGGGA GGTGCATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA TAATGCTTTG
    GAAACGGCCA CTGGCAACTT TGTGTCATTA CATGAGTGGG TCCTTCGGTG GCAGAAAAAA
    ATGGGCCCGT TCCTTACCAG TCAAGAGAAG GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACGGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC
    TACTTTGCAA TGAATCTCAA AGAGCAAGAT GACTCCATCA TGGTGGACAT TTTATTGGAG
    CACATTAATG GGGTCTCCCT CTCTGCACAT CTGAGCCACT CAGGCCCCAT CCCTGTGTAT
    CAACTCCGCA AGTACACAGC TCAGCTCTTG TCTGGCCTCG ATTATTTGCA CAGCAATGAC
    GTGGTGCACA AGGTTCTGAA TGCATCAAAT GTCTTGGTGG ATGCAGAGGG CAATGTCAAG
    ATTACGGACT ACAGCATTTC AAAGCGCCTA GCAGACATTT GCAAGGAGGA TGTGTTTGAG
    CAGAGCCGAG TTAGTTTTAG TGACAGTGCC CTACCTTATA AAACGGGGAA GAAAGGGGAT
    GTTTGGTGTC TTGGCCTTCT GCTGCTCTTC CTCAGCCAAG GACAAGAATG CGGAGAGTAC
    CCTGTGACCA TCCCTAGCGA CTTACCAGCT GACTTCCAAG ATTTTCTAAA GAAGTGTGTT
    TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAATTGT TGAAACACAG CTTTATCAAT
    CCACAGCCGA AAATGTCTTT ATTGGAACAG AGTCCTGACG ATTCTGGAGG ACAAGATTAT
    GTTGAGACTG TTATTCCTAG TAAGCAGCTA CCCAGTGCAG CATTCTTCAG TGAGACACAG
    AGACAGTTTT CCCGGTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCC
    TTCGGAGCTG TCATCAAGGT GCAGAACAAG CTGGATGGCT GCTGCTACGC GGTGAAGCGC
    ATCCCCATCA ATCCGGCCAG TAGGCATTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTGC ACCACGAGAA CATAGTGCGC TACTATAACG CCTGGATAGA GAGATACGAG
    CGGCCCGTGG GCCCGGGGAT GCTGCCGCAG GACTCGGACT CATGGCCCCA GGCACCGGAT
    GGGCGAGCTT TGTCCCAGCC GTCCCCAGGC AACGACAGCC CCGACGGCGA CGGCGCCGGT
    AGCGTGGAGG CCGCTGCGCC ACCGCCCATC CTCAGCTCGG TGGAGTGGAG CACATCTGGC
    GAGCGTTCGG CCAGCGCCCG CTTCCCCTCC GCCCGCCCAG GCTCCAGCAG CGAGGAGGAC
    GACGACGACG ACGAGCACGA GGGCGTCTTC TCCCAGCCCT TCCTACCTGC TTCAGATTCT
    GACAGTGATG TCATCTTTGA AGATGAGAAC AGTAAGAGTC AGAATCAGGA AGAAGACTGC
    AATGGGAAGA ACAGTTGCCA TGAAAGTGAG CCATCGACAA CCACTGAAGC TGTGCACTAC
    CTGTATATCC AGATGGAGTA CTGTGAGAAG AGTACTTTGC GTGACACCAT TGACCAGGGG
    CTATATCAAG ATACCATCAG ACTCTGGAGG CTTTTTCGAG AGATTCTGGA TGGATTAGCT
    TATATCCATG AAAAAGGAAT GATTCACCGG GATTTGAAGC CTGTCAACAT CTTTTTGGAT
    TCTGATGACC ATGTAAAAAT AGGTGATTTT GGTTTAGCGA CAGACCATCT TGCCTTCACT
    GCTGATGGCA AGCAAGATGA TACAGCAGAT CACTTGATTA AATCAGACCC CTCAGGTCAT
    TTGACTGGGA TGGTTGGTAC TGCTTTATAC GTAAGCCCAG AGGTCCAAGG AAGCAGCAGA
    CCCACGTACA ACCAGAAAGT GGATCTCTTC AGCCTGGGAA TTATCTTCTT TGAGATGTCC
    TATCACCCCA TGGTCACGGC CTCAGAAAGG ATCTTTGTTC TCAACCAACT CAGAGATCCC
    ACTTCACCTA AGTTTCCAGA AGACTTCGAT GATGGAGAGC ATACAAAGCA GAAATCTGTC
    ATCTCCTGGC TGTTGAACCA TGATCCCGCC AAGCGGCCCA CAGCCACAGA GCTGCTCAAG
    AGTGAGCTGC TGCCCCCACC CCAGATGGAG GAGTCAGAGT TGCATGAGGT TCTGCACCAC
    ACCCTGGCCA ACGTGGATGG GAAGGCCTAC CGCACCATGA TGGCCCAGAT CTTCTCCCAG
    CGCGTCAGTC CAGCCATCGA TTACACCTAT GACAGCAACA TGCCGAAGGG CTTTTCAATC
    CGTACATCCA AGATACAGCA ACATGTGTGT GATACCATCG TCCGCATTTT TAAAAAACAC
    GGAGCTGTCC AGTTGTGTAC TCCACTACTG CTTCCCCAAA ATAGGCAAAT ATACGAGCAC
    AATGAAGCTG CCTTATTCAT GGACCACAGC GGGATGCTGG TGATGCTTCC CTTTGACCTA
    CGGGTTCCTT TTGCAAGATA TGTGGCAAGA AATAATATAT TGAATTTGAA ACGTTACTGC
    ATAGAACGTG TCTTCAGACC TCGCAAGTTA GACCGATTTC ATCCCAAAGA ACTTCTGGAA
    TGTGCATTTG ACATTGTTAC GTCTACCACC AACAGCTCTC TGCCCACTGC TGAAGTCATC
    TACACGATCT GTGAGATCAT CCAGGAGTTC CCGGCACTGC AGGAAAGAAA TTACAGTATT
    TACTTGAACC ACACCATGTT ATTGAAAGCA ATCTTCTTAC ACTGTGGGAT CCCAGAAGAT
    AAACTCAGTC AAGTCTATGT TATTCTATAT GATGCAGTGA CAGAAAAGCT GACAAGGAGA
    GAAGTGGAAG CCAAGTTTTG TAATCTGTCT CTGTCATCTA ATAGTCTGTG TCGACTCTAC
    AAATTCATTG AGCAGAAGGG AGATTTGCAA GATCTAACAC CAGCAATAAA TTCATTAATA
    AAACAGAAAA CAGGTGTTGC CCAACTGGTG AAGTATGGCT TGAAAGACTT AGAGGAGATC
    ATTGGGCTGT TGAAGAAACT CGGCATCAAG TTACAGGTCT TGATCAACTT GGGCTTGGTT
    TACAAAGTAC AGCAGCACAA CGGAGTCATC TTCCAGTTTG TGGCGGTCAT CAGACGGAGG
    CAAAGGGCTA TTTCAGAAAT CCTTGCAGCT GGAGGCAGAT ACGACCTGCT GATTCCCCAG
    TTTAGAGGAC CGCAGGCTCT GGGACCAGTC CCGACTGCTG TTGGGGTCAG CATAGCCATA
    GACAAGATAT CTGCAGCTAT CCTCAACATG GAAGAACCTG TTACAATAAG CTCTTGTGAC
    CTCCTGATCG TAAGTGTTGG CCAGATGTCC ATGTCCAGGG CCATCAACCT AACCCAGAAA
    CTTTGGTCAG CAGGAATCAC AGCACAGATC ATGTACGACT GGTCACAGTC CCAAGAAGAA
    CTCCAGGAAT ACTGCAGATA CCACGAAATC ACCTACGTGG CCCTCGTCTC AGATAAAGGA
    GGAAGCTATG TCAAGAGAAG CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638288.1
    CDS92..4498
    Translation

    Target ORF information:

    RefSeq Version XM_005638231.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638231.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
    CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC
    CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA
    CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT
    GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG
    GTGATGATAT TTGAACTGGC CGACCATGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC
    CCTCCCAAGT CTTTCCATGA GGAAATGCTA GAAAGGCGGG CTCAGGAGGA GCAGCGGAGG
    CTGTTGGAGG CCAAGCAGAA GGTAGAGCAG GAGCAACGTG AAATCCTTCA TGAGATTCAA
    AGAAGGAAAG AAGAGATAAA AGAAGAGAGA AAAAGAAAAG AAATGGCTAA GCAGGAGCGT
    TTGGAAATTG CTAGTTTGTC AAACCAAGAT CAAAACTCTA GGAAAGACCC AGGAGGACAC
    AGAATATCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAGGCGAGAA CGTCAGTATT CTCTGTGTAG TAGTGAAGAT
    TCTTCTGGCT CTTGTGAAGT CCTGTATTTC TCTATGGGTA GCCCTGATCA GCTCATGGTT
    TACAAAGGGA GGTGCATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA TAATGCTTTG
    GAAACGGCCA CTGGCAACTT TGTGTCATTA CATGAGTGGG TCCTTCGGTG GCAGAAAAAA
    ATGGGCCCGT TCCTTACCAG TCAAGAGAAG GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACGGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC
    TACTTTGCAA TGAATCTCAA AGAGCAAGAT GACTCCATCA TGGTGGACAT TTTATTGGAG
    CACATTAATG GGGTCTCCCT CTCTGCACAT CTGAGCCACT CAGGCCCCAT CCCTGTGTAT
    CAACTCCGCA AGTACACAGC TCAGCTCTTG TCTGGCCTCG ATTATTTGCA CAGCAATGAC
    GTGGTGCACA AGGTTCTGAA TGCATCAAAT GTCTTGGTGG ATGCAGAGGG CAATGTCAAG
    ATTACGGACT ACAGCATTTC AAAGCGCCTA GCAGACATTT GCAAGGAGGA TGTGTTTGAG
    CAGAGCCGAG TTAGTTTTAG TGACAGTGCC CTACCTTATA AAACGGGGAA GAAAGGGGAT
    GTTTGGTGTC TTGGCCTTCT GCTGCTCTTC CTCAGCCAAG GACAAGAATG CGGAGAGTAC
    CCTGTGACCA TCCCTAGCGA CTTACCAGCT GACTTCCAAG ATTTTCTAAA GAAGTGTGTT
    TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAATTGT TGAAACACAG CTTTATCAAT
    CCACAGCCGA AAATGTCTTT ATTGGAACAG AGTCCTGACG ATTCTGGAGG ACAAGATTAT
    GTTGAGACTG TTATTCCTAG TAAGCAGCTA CCCAGTGCAG CATTCTTCAG TGAGACACAG
    AGACAGTTTT CCCGGTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCC
    TTCGGAGCTG TCATCAAGGT GCAGAACAAG CTGGATGGCT GCTGCTACGC GGTGAAGCGC
    ATCCCCATCA ATCCGGCCAG TAGGCATTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTGC ACCACGAGAA CATAGTGCGC TACTATAACG CCTGGATAGA GAGATACGAG
    CGGCCCGTGG GCCCGGGGAT GCTGCCGCAG GACTCGGACT CATGGCCCCA GGCACCGGAT
    GGGCGAGCTT TGTCCCAGCC GTCCCCAGGC AACGACAGCC CCGACGGCGA CGGCGCCGGT
    AGCGTGGAGG CCGCTGCGCC ACCGCCCATC CTCAGCTCGG TGGAGTGGAG CACATCTGGC
    GAGCGTTCGG CCAGCGCCCG CTTCCCCTCC GCCCGCCCAG GCTCCAGCAG CGAGGAGGAC
    GACGACGACG ACGAGCACGA GGGCGTCTTC TCCCAGCCCT TCCTACCTGC TTCAGATTCT
    GACAGTGATG TCATCTTTGA AGATGAGAAC AGTAAGAGTC AGAATCAGGA AGAAGACTGC
    AATGGGAAGA ACAGTTGCCA TGAAAGTGAG CCATCGACAA CCACTGAAGC TGTGCACTAC
    CTGTATATCC AGATGGAGTA CTGTGAGAAG AGTACTTTGC GTGACACCAT TGACCAGGGG
    CTATATCAAG ATACCATCAG ACTCTGGAGG CTTTTTCGAG AGATTCTGGA TGGATTAGCT
    TATATCCATG AAAAAGGAAT GATTCACCGG GATTTGAAGC CTGTCAACAT CTTTTTGGAT
    TCTGATGACC ATGTAAAAAT AGGTGATTTT GGTTTAGCGA CAGACCATCT TGCCTTCACT
    GCTGATGGCA AGCAAGATGA TACAGCAGAT CACTTGATTA AATCAGACCC CTCAGGTCAT
    TTGACTGGGA TGGTTGGTAC TGCTTTATAC GTAAGCCCAG AGGTCCAAGG AAGCAGCAGA
    CCCACGTACA ACCAGAAAGT GGATCTCTTC AGCCTGGGAA TTATCTTCTT TGAGATGTCC
    TATCACCCCA TGGTCACGGC CTCAGAAAGG ATCTTTGTTC TCAACCAACT CAGAGATCCC
    ACTTCACCTA AGTTTCCAGA AGACTTCGAT GATGGAGAGC ATACAAAGCA GAAATCTGTC
    ATCTCCTGGC TGTTGAACCA TGATCCCGCC AAGCGGCCCA CAGCCACAGA GCTGCTCAAG
    AGTGAGCTGC TGCCCCCACC CCAGATGGAG GAGTCAGAGT TGCATGAGGT TCTGCACCAC
    ACCCTGGCCA ACGTGGATGG GAAGGCCTAC CGCACCATGA TGGCCCAGAT CTTCTCCCAG
    CGCGTCAGTC CAGCCATCGA TTACACCTAT GACAGCAACA TGCCGAAGGG CTTTTCAATC
    CGTACATCCA AGATACAGCA ACATGTGTGT GATACCATCG TCCGCATTTT TAAAAAACAC
    GGAGCTGTCC AGTTGTGTAC TCCACTACTG CTTCCCCAAA ATAGGCAAAT ATACGAGCAC
    AATGAAGCTG CCTTATTCAT GGACCACAGC GGGATGCTGG TGATGCTTCC CTTTGACCTA
    CGGGTTCCTT TTGCAAGATA TGTGGCAAGA AATAATATAT TGAATTTGAA ACGTTACTGC
    ATAGAACGTG TCTTCAGACC TCGCAAGTTA GACCGATTTC ATCCCAAAGA ACTTCTGGAA
    TGTGCATTTG ACATTGTTAC GTCTACCACC AACAGCTCTC TGCCCACTGC TGAAGTCATC
    TACACGATCT GTGAGATCAT CCAGGAGTTC CCGGCACTGC AGGAAAGAAA TTACAGTATT
    TACTTGAACC ACACCATGTT ATTGAAAGCA ATCTTCTTAC ACTGTGGGAT CCCAGAAGAT
    AAACTCAGTC AAGTCTATGT TATTCTATAT GATGCAGTGA CAGAAAAGCT GACAAGGAGA
    GAAGTGGAAG CCAAGTTTTG TAATCTGTCT CTGTCATCTA ATAGTCTGTG TCGACTCTAC
    AAATTCATTG AGCAGAAGGG AGATTTGCAA GATCTAACAC CAGCAATAAA TTCATTAATA
    AAACAGAAAA CAGGTGTTGC CCAACTGGTG AAGTATGGCT TGAAAGACTT AGAGGAGATC
    ATTGGGCTGT TGAAGAAACT CGGCATCAAG TTACAGGTCT TGATCAACTT GGGCTTGGTT
    TACAAAGTAC AGCAGCACAA CGGAGTCATC TTCCAGTTTG TGGCGGTCAT CAGACGGAGG
    CAAAGGGCTA TTTCAGAAAT CCTTGCAGCT GGAGGCAGAT ACGACCTGCT GATTCCCCAG
    TTTAGAGGAC CGCAGGCTCT GGGACCAGTC CCGACTGCTG TTGGGGTCAG CATAGCCATA
    GACAAGATAT CTGCAGCTAT CCTCAACATG GAAGAACCTG TTACAATAAG CTCTTGTGAC
    CTCCTGATCG TAAGTGTTGG CCAGATGTCC ATGTCCAGGG CCATCAACCT AACCCAGAAA
    CTTTGGTCAG CAGGAATCAC AGCACAGATC ATGTACGACT GGTCACAGTC CCAAGAAGAA
    CTCCAGGAAT ACTGCAGATA CCACGAAATC ACCTACGTGG CCCTCGTCTC AGATAAAGGA
    GGAAGCTATG TCAAGAGAAG CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe28639
    Clone ID Related Accession (Same CDS sequence) XM_005638231.2 , XM_005638231.3
    Accession Version XM_005638231.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product eIF-2-alpha kinase GCN2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638231.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
    CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC
    CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA
    CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT
    GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG
    GTGATGATAT TTGAACTGGC CGACCATGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC
    CCTCCCAAGT CTTTCCATGA GGAAATGCTA GAAAGGCGGG CTCAGGAGGA GCAGCGGAGG
    CTGTTGGAGG CCAAGCAGAA GGTAGAGCAG GAGCAACGTG AAATCCTTCA TGAGATTCAA
    AGAAGGAAAG AAGAGATAAA AGAAGAGAGA AAAAGAAAAG AAATGGCTAA GCAGGAGCGT
    TTGGAAATTG CTAGTTTGTC AAACCAAGAT CAAAACTCTA GGAAAGACCC AGGAGGACAC
    AGAATATCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAGGCGAGAA CGTCAGTATT CTCTGTGTAG TAGTGAAGAT
    TCTTCTGGCT CTTGTGAAGT CCTGTATTTC TCTATGGGTA GCCCTGATCA GCTCATGGTT
    TACAAAGGGA GGTGCATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA TAATGCTTTG
    GAAACGGCCA CTGGCAACTT TGTGTCATTA CATGAGTGGG TCCTTCGGTG GCAGAAAAAA
    ATGGGCCCGT TCCTTACCAG TCAAGAGAAG GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACGGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC
    TACTTTGCAA TGAATCTCAA AGAGCAAGAT GACTCCATCA TGGTGGACAT TTTATTGGAG
    CACATTAATG GGGTCTCCCT CTCTGCACAT CTGAGCCACT CAGGCCCCAT CCCTGTGTAT
    CAACTCCGCA AGTACACAGC TCAGCTCTTG TCTGGCCTCG ATTATTTGCA CAGCAATGAC
    GTGGTGCACA AGGTTCTGAA TGCATCAAAT GTCTTGGTGG ATGCAGAGGG CAATGTCAAG
    ATTACGGACT ACAGCATTTC AAAGCGCCTA GCAGACATTT GCAAGGAGGA TGTGTTTGAG
    CAGAGCCGAG TTAGTTTTAG TGACAGTGCC CTACCTTATA AAACGGGGAA GAAAGGGGAT
    GTTTGGTGTC TTGGCCTTCT GCTGCTCTTC CTCAGCCAAG GACAAGAATG CGGAGAGTAC
    CCTGTGACCA TCCCTAGCGA CTTACCAGCT GACTTCCAAG ATTTTCTAAA GAAGTGTGTT
    TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAATTGT TGAAACACAG CTTTATCAAT
    CCACAGCCGA AAATGTCTTT ATTGGAACAG AGTCCTGACG ATTCTGGAGG ACAAGATTAT
    GTTGAGACTG TTATTCCTAG TAAGCAGCTA CCCAGTGCAG CATTCTTCAG TGAGACACAG
    AGACAGTTTT CCCGGTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCC
    TTCGGAGCTG TCATCAAGGT GCAGAACAAG CTGGATGGCT GCTGCTACGC GGTGAAGCGC
    ATCCCCATCA ATCCGGCCAG TAGGCATTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTGC ACCACGAGAA CATAGTGCGC TACTATAACG CCTGGATAGA GAGATACGAG
    CGGCCCGTGG GCCCGGGGAT GCTGCCGCAG GACTCGGACT CATGGCCCCA GGCACCGGAT
    GGGCGAGCTT TGTCCCAGCC GTCCCCAGGC AACGACAGCC CCGACGGCGA CGGCGCCGGT
    AGCGTGGAGG CCGCTGCGCC ACCGCCCATC CTCAGCTCGG TGGAGTGGAG CACATCTGGC
    GAGCGTTCGG CCAGCGCCCG CTTCCCCTCC GCCCGCCCAG GCTCCAGCAG CGAGGAGGAC
    GACGACGACG ACGAGCACGA GGGCGTCTTC TCCCAGCCCT TCCTACCTGC TTCAGATTCT
    GACAGTGATG TCATCTTTGA AGATGAGAAC AGTAAGAGTC AGAATCAGGA AGAAGACTGC
    AATGGGAAGA ACAGTTGCCA TGAAAGTGAG CCATCGACAA CCACTGAAGC TGTGCACTAC
    CTGTATATCC AGATGGAGTA CTGTGAGAAG AGTACTTTGC GTGACACCAT TGACCAGGGG
    CTATATCAAG ATACCATCAG ACTCTGGAGG CTTTTTCGAG AGATTCTGGA TGGATTAGCT
    TATATCCATG AAAAAGGAAT GATTCACCGG GATTTGAAGC CTGTCAACAT CTTTTTGGAT
    TCTGATGACC ATGTAAAAAT AGGTGATTTT GGTTTAGCGA CAGACCATCT TGCCTTCACT
    GCTGATGGCA AGCAAGATGA TACAGCAGAT CACTTGATTA AATCAGACCC CTCAGGTCAT
    TTGACTGGGA TGGTTGGTAC TGCTTTATAC GTAAGCCCAG AGGTCCAAGG AAGCAGCAGA
    CCCACGTACA ACCAGAAAGT GGATCTCTTC AGCCTGGGAA TTATCTTCTT TGAGATGTCC
    TATCACCCCA TGGTCACGGC CTCAGAAAGG ATCTTTGTTC TCAACCAACT CAGAGATCCC
    ACTTCACCTA AGTTTCCAGA AGACTTCGAT GATGGAGAGC ATACAAAGCA GAAATCTGTC
    ATCTCCTGGC TGTTGAACCA TGATCCCGCC AAGCGGCCCA CAGCCACAGA GCTGCTCAAG
    AGTGAGCTGC TGCCCCCACC CCAGATGGAG GAGTCAGAGT TGCATGAGGT TCTGCACCAC
    ACCCTGGCCA ACGTGGATGG GAAGGCCTAC CGCACCATGA TGGCCCAGAT CTTCTCCCAG
    CGCGTCAGTC CAGCCATCGA TTACACCTAT GACAGCAACA TGCCGAAGGG CTTTTCAATC
    CGTACATCCA AGATACAGCA ACATGTGTGT GATACCATCG TCCGCATTTT TAAAAAACAC
    GGAGCTGTCC AGTTGTGTAC TCCACTACTG CTTCCCCAAA ATAGGCAAAT ATACGAGCAC
    AATGAAGCTG CCTTATTCAT GGACCACAGC GGGATGCTGG TGATGCTTCC CTTTGACCTA
    CGGGTTCCTT TTGCAAGATA TGTGGCAAGA AATAATATAT TGAATTTGAA ACGTTACTGC
    ATAGAACGTG TCTTCAGACC TCGCAAGTTA GACCGATTTC ATCCCAAAGA ACTTCTGGAA
    TGTGCATTTG ACATTGTTAC GTCTACCACC AACAGCTCTC TGCCCACTGC TGAAGTCATC
    TACACGATCT GTGAGATCAT CCAGGAGTTC CCGGCACTGC AGGAAAGAAA TTACAGTATT
    TACTTGAACC ACACCATGTT ATTGAAAGCA ATCTTCTTAC ACTGTGGGAT CCCAGAAGAT
    AAACTCAGTC AAGTCTATGT TATTCTATAT GATGCAGTGA CAGAAAAGCT GACAAGGAGA
    GAAGTGGAAG CCAAGTTTTG TAATCTGTCT CTGTCATCTA ATAGTCTGTG TCGACTCTAC
    AAATTCATTG AGCAGAAGGG AGATTTGCAA GATCTAACAC CAGCAATAAA TTCATTAATA
    AAACAGAAAA CAGGTGTTGC CCAACTGGTG AAGTATGGCT TGAAAGACTT AGAGGAGATC
    ATTGGGCTGT TGAAGAAACT CGGCATCAAG TTACAGGTCT TGATCAACTT GGGCTTGGTT
    TACAAAGTAC AGCAGCACAA CGGAGTCATC TTCCAGTTTG TGGCGGTCAT CAGACGGAGG
    CAAAGGGCTA TTTCAGAAAT CCTTGCAGCT GGAGGCAGAT ACGACCTGCT GATTCCCCAG
    TTTAGAGGAC CGCAGGCTCT GGGACCAGTC CCGACTGCTG TTGGGGTCAG CATAGCCATA
    GACAAGATAT CTGCAGCTAT CCTCAACATG GAAGAACCTG TTACAATAAG CTCTTGTGAC
    CTCCTGATCG TAAGTGTTGG CCAGATGTCC ATGTCCAGGG CCATCAACCT AACCCAGAAA
    CTTTGGTCAG CAGGAATCAC AGCACAGATC ATGTACGACT GGTCACAGTC CCAAGAAGAA
    CTCCAGGAAT ACTGCAGATA CCACGAAATC ACCTACGTGG CCCTCGTCTC AGATAAAGGA
    GGAAGCTATG TCAAGAGAAG CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005638288.1
    CDS59..4465
    Translation

    Target ORF information:

    RefSeq Version XM_005638231.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005638231.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
    CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC
    CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC
    CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA
    CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT
    GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG
    GTGATGATAT TTGAACTGGC CGACCATGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC
    CCTCCCAAGT CTTTCCATGA GGAAATGCTA GAAAGGCGGG CTCAGGAGGA GCAGCGGAGG
    CTGTTGGAGG CCAAGCAGAA GGTAGAGCAG GAGCAACGTG AAATCCTTCA TGAGATTCAA
    AGAAGGAAAG AAGAGATAAA AGAAGAGAGA AAAAGAAAAG AAATGGCTAA GCAGGAGCGT
    TTGGAAATTG CTAGTTTGTC AAACCAAGAT CAAAACTCTA GGAAAGACCC AGGAGGACAC
    AGAATATCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG
    ACCAACTCCT CTGGAAGGTC TAGGCGAGAA CGTCAGTATT CTCTGTGTAG TAGTGAAGAT
    TCTTCTGGCT CTTGTGAAGT CCTGTATTTC TCTATGGGTA GCCCTGATCA GCTCATGGTT
    TACAAAGGGA GGTGCATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA TAATGCTTTG
    GAAACGGCCA CTGGCAACTT TGTGTCATTA CATGAGTGGG TCCTTCGGTG GCAGAAAAAA
    ATGGGCCCGT TCCTTACCAG TCAAGAGAAG GAGAAGATTG ATAAGTGCAA AAAGCAGATT
    CAAGGGGCAG AAACGGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC
    TACTTTGCAA TGAATCTCAA AGAGCAAGAT GACTCCATCA TGGTGGACAT TTTATTGGAG
    CACATTAATG GGGTCTCCCT CTCTGCACAT CTGAGCCACT CAGGCCCCAT CCCTGTGTAT
    CAACTCCGCA AGTACACAGC TCAGCTCTTG TCTGGCCTCG ATTATTTGCA CAGCAATGAC
    GTGGTGCACA AGGTTCTGAA TGCATCAAAT GTCTTGGTGG ATGCAGAGGG CAATGTCAAG
    ATTACGGACT ACAGCATTTC AAAGCGCCTA GCAGACATTT GCAAGGAGGA TGTGTTTGAG
    CAGAGCCGAG TTAGTTTTAG TGACAGTGCC CTACCTTATA AAACGGGGAA GAAAGGGGAT
    GTTTGGTGTC TTGGCCTTCT GCTGCTCTTC CTCAGCCAAG GACAAGAATG CGGAGAGTAC
    CCTGTGACCA TCCCTAGCGA CTTACCAGCT GACTTCCAAG ATTTTCTAAA GAAGTGTGTT
    TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAATTGT TGAAACACAG CTTTATCAAT
    CCACAGCCGA AAATGTCTTT ATTGGAACAG AGTCCTGACG ATTCTGGAGG ACAAGATTAT
    GTTGAGACTG TTATTCCTAG TAAGCAGCTA CCCAGTGCAG CATTCTTCAG TGAGACACAG
    AGACAGTTTT CCCGGTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCC
    TTCGGAGCTG TCATCAAGGT GCAGAACAAG CTGGATGGCT GCTGCTACGC GGTGAAGCGC
    ATCCCCATCA ATCCGGCCAG TAGGCATTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG
    TCGCGCCTGC ACCACGAGAA CATAGTGCGC TACTATAACG CCTGGATAGA GAGATACGAG
    CGGCCCGTGG GCCCGGGGAT GCTGCCGCAG GACTCGGACT CATGGCCCCA GGCACCGGAT
    GGGCGAGCTT TGTCCCAGCC GTCCCCAGGC AACGACAGCC CCGACGGCGA CGGCGCCGGT
    AGCGTGGAGG CCGCTGCGCC ACCGCCCATC CTCAGCTCGG TGGAGTGGAG CACATCTGGC
    GAGCGTTCGG CCAGCGCCCG CTTCCCCTCC GCCCGCCCAG GCTCCAGCAG CGAGGAGGAC
    GACGACGACG ACGAGCACGA GGGCGTCTTC TCCCAGCCCT TCCTACCTGC TTCAGATTCT
    GACAGTGATG TCATCTTTGA AGATGAGAAC AGTAAGAGTC AGAATCAGGA AGAAGACTGC
    AATGGGAAGA ACAGTTGCCA TGAAAGTGAG CCATCGACAA CCACTGAAGC TGTGCACTAC
    CTGTATATCC AGATGGAGTA CTGTGAGAAG AGTACTTTGC GTGACACCAT TGACCAGGGG
    CTATATCAAG ATACCATCAG ACTCTGGAGG CTTTTTCGAG AGATTCTGGA TGGATTAGCT
    TATATCCATG AAAAAGGAAT GATTCACCGG GATTTGAAGC CTGTCAACAT CTTTTTGGAT
    TCTGATGACC ATGTAAAAAT AGGTGATTTT GGTTTAGCGA CAGACCATCT TGCCTTCACT
    GCTGATGGCA AGCAAGATGA TACAGCAGAT CACTTGATTA AATCAGACCC CTCAGGTCAT
    TTGACTGGGA TGGTTGGTAC TGCTTTATAC GTAAGCCCAG AGGTCCAAGG AAGCAGCAGA
    CCCACGTACA ACCAGAAAGT GGATCTCTTC AGCCTGGGAA TTATCTTCTT TGAGATGTCC
    TATCACCCCA TGGTCACGGC CTCAGAAAGG ATCTTTGTTC TCAACCAACT CAGAGATCCC
    ACTTCACCTA AGTTTCCAGA AGACTTCGAT GATGGAGAGC ATACAAAGCA GAAATCTGTC
    ATCTCCTGGC TGTTGAACCA TGATCCCGCC AAGCGGCCCA CAGCCACAGA GCTGCTCAAG
    AGTGAGCTGC TGCCCCCACC CCAGATGGAG GAGTCAGAGT TGCATGAGGT TCTGCACCAC
    ACCCTGGCCA ACGTGGATGG GAAGGCCTAC CGCACCATGA TGGCCCAGAT CTTCTCCCAG
    CGCGTCAGTC CAGCCATCGA TTACACCTAT GACAGCAACA TGCCGAAGGG CTTTTCAATC
    CGTACATCCA AGATACAGCA ACATGTGTGT GATACCATCG TCCGCATTTT TAAAAAACAC
    GGAGCTGTCC AGTTGTGTAC TCCACTACTG CTTCCCCAAA ATAGGCAAAT ATACGAGCAC
    AATGAAGCTG CCTTATTCAT GGACCACAGC GGGATGCTGG TGATGCTTCC CTTTGACCTA
    CGGGTTCCTT TTGCAAGATA TGTGGCAAGA AATAATATAT TGAATTTGAA ACGTTACTGC
    ATAGAACGTG TCTTCAGACC TCGCAAGTTA GACCGATTTC ATCCCAAAGA ACTTCTGGAA
    TGTGCATTTG ACATTGTTAC GTCTACCACC AACAGCTCTC TGCCCACTGC TGAAGTCATC
    TACACGATCT GTGAGATCAT CCAGGAGTTC CCGGCACTGC AGGAAAGAAA TTACAGTATT
    TACTTGAACC ACACCATGTT ATTGAAAGCA ATCTTCTTAC ACTGTGGGAT CCCAGAAGAT
    AAACTCAGTC AAGTCTATGT TATTCTATAT GATGCAGTGA CAGAAAAGCT GACAAGGAGA
    GAAGTGGAAG CCAAGTTTTG TAATCTGTCT CTGTCATCTA ATAGTCTGTG TCGACTCTAC
    AAATTCATTG AGCAGAAGGG AGATTTGCAA GATCTAACAC CAGCAATAAA TTCATTAATA
    AAACAGAAAA CAGGTGTTGC CCAACTGGTG AAGTATGGCT TGAAAGACTT AGAGGAGATC
    ATTGGGCTGT TGAAGAAACT CGGCATCAAG TTACAGGTCT TGATCAACTT GGGCTTGGTT
    TACAAAGTAC AGCAGCACAA CGGAGTCATC TTCCAGTTTG TGGCGGTCAT CAGACGGAGG
    CAAAGGGCTA TTTCAGAAAT CCTTGCAGCT GGAGGCAGAT ACGACCTGCT GATTCCCCAG
    TTTAGAGGAC CGCAGGCTCT GGGACCAGTC CCGACTGCTG TTGGGGTCAG CATAGCCATA
    GACAAGATAT CTGCAGCTAT CCTCAACATG GAAGAACCTG TTACAATAAG CTCTTGTGAC
    CTCCTGATCG TAAGTGTTGG CCAGATGTCC ATGTCCAGGG CCATCAACCT AACCCAGAAA
    CTTTGGTCAG CAGGAATCAC AGCACAGATC ATGTACGACT GGTCACAGTC CCAAGAAGAA
    CTCCAGGAAT ACTGCAGATA CCACGAAATC ACCTACGTGG CCCTCGTCTC AGATAAAGGA
    GGAAGCTATG TCAAGAGAAG CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.