SDHB cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SDHB
Entrez Gene ID	478217
Full Name	succinate dehydrogenase complex iron sulfur subunit B
General protein information	Preferred Names succinate dehydrogenase complex iron sulfur subunit B Names succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial succinate dehydrogenase complex subunit B succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001252217.1	NP_001239146.1	succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007309	XP_308512.4
Kluyveromyces lactis	KLLA0E00683g	XP_453977.1
	SDHB	XP_001088349.1
Saccharomyces cerevisiae (baker's yeast)	SDH2	NP_013059.1
Eremothecium gossypii	AGOS_ACL065C	NP_983339.1
Neurospora crassa	NCU00959	XP_965152.1
Bos taurus (cattle)	SDHB	NP_001035573.1
Homo sapiens (human)	SDHB	NP_002991.2
Mus musculus (house mouse)	Sdhb	NP_075863.2
Rattus norvegicus (Norway rat)	Sdhb	NP_001094009.1
Gallus gallus (chicken)	SDHB	XP_004950147.1
Danio rerio (zebrafish)	sdhb	NP_001092210.1
Drosophila melanogaster (fruit fly)	SdhB	NP_477101.1
Arabidopsis thaliana (thale cress)	SDH2-1	NP_001118718.1
Arabidopsis thaliana (thale cress)	SDH2-2	NP_198881.1
Oryza sativa (rice)	Os08g0120000	NP_001060876.1
Xenopus tropicalis (tropical clawed frog)	sdhb	NP_001120000.1
Pan troglodytes (chimpanzee)	SDHB	XP_003949355.1
Canis lupus familiaris (dog)	SDHB	NP_001239146.1
Caenorhabditis elegans (roundworm)	sdhb-1	NP_495992.1
Schizosaccharomyces pombe (fission yeast)	sdh2	NP_593530.2
Magnaporthe oryzae (rice blast fungus)	MGG_00167	XP_003718958.1

Gene Ontology
Bibliography

Related articles in PubMed

Succinate dehydrogenase subunit D and succinate dehydrogenase subunit B mutation analysis in canine phaeochromocytoma and paraganglioma.
Holt DE, Henthorn P, Howell VM, Robinson BG, Benn DE
Journal of comparative pathology151(1)25-34(2014 Jul)

Housekeeping genes for phylogenetic analysis of eutherian relationships.
Kullberg M, Nilsson MA, Arnason U, Harley EH, Janke A
Molecular biology and evolution23(8)1493-503(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genetic mutations in SDHD and SDHB may underlie tumourigenesis in canine Phaeochromocytomas and paragangliomas
Title: Succinate dehydrogenase subunit D and succinate dehydrogenase subunit B mutation analysis in canine phaeochromocytoma and paraganglioma.

The following SDHB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SDHB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe34204	NM_001252217.1 Latest version!	Canis lupus familiaris succinate dehydrogenase complex iron sulfur subunit B (SDHB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SDHB ( NM_001252217.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001239146.1 Canis lupus familiaris succinate dehydrogenase complex iron sulfur subunit B (SDHB), mRNA.

Price & Availability↑

CloneID	OCe34204
Clone ID Related Accession (Same CDS sequence)	NM_001252217.1
Accession Version	NM_001252217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DN393350.1, DN434916.1 and DN904525.1. On Nov 10, 2011 this sequence version replaced XM_535392.3. ##Evidence-Data-START## Transcript exon combination :: DN392571.1, DN434916.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG TGGTCGGAGT CTCCTTGAAA CGCCGGTTCC CGGCTGCAGC CCTCGGCGGA 
GCCTGCCTGC AGGCCTGCCG GGGGGCACAG ACAGCGGCAG CCACAGCTCC CCGAATCAAG 
AAATTTGCCA TCTACCGATG GGACCCAGAC AAGACTGGAG ATAAACCGCA TATGCAGACT 
TATGAAATTG ATTTGAATAA ATGTGGTCCT ATGGTGTTGG ATGCTTTAAT CAAGATTAAG 
AATGAAATTG ACTCTACCTT GACCTTCCGA AGATCATGCA GAGAAGGCAT CTGTGGCTCC 
TGTGCCATGA ACATCAACGG AGGCAACACT CTGGCTTGCA CCCGCCGGAT CGACACCAAC 
CTCAGCAAAG TCTCCAAAAT CTACCCTCTG CCGCATATGT ATGTGATAAA GGATCTGGTT 
CCTGACTTGA GCAACTTCTA TGCTCAGTAT AAATCCATTG AACCTTATTT GAAGAAGAAG 
GATGAATCCC AAGAGGGCAA GCAGCAGTAT CTGCAGTCCA TAGAAGATCG TGAGAAACTG 
GACGGGCTGT ACGAGTGTAT CCTCTGTGCT TGCTGCAGCA CCAGCTGCCC TAGCTACTGG 
TGGAATGGAG ACAAATACCT GGGGCCTGCG GTTCTCATGC AGGCCTACCG CTGGATGATC 
GACTCCAGAG ACGACTTCAC TGAGGAGCGC CTGGCCAAGC TGCAGGACCC GTTCTCTCTG 
TACCGCTGCC ACACCATCAT GAACTGCACA AGGACCTGCC CCAAGGGGCT GAATCCAGGC 
AAAGCTATTG CTGAAATCAA GAAAATGATG GCCACCTATA AGGGGAAGAA AGCGTCCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239146.1
CDS	14..856
Translation	MAAVVGVSLKRRFPAAALGGACLQACRGAQTAAATAPRIKKFAIYRWDPDKTGDKPHMQTYEIDLNKCGPMVLDALIKIKNEIDSTLTFRRSCREGICGSCAMNINGGNTLACTRRIDTNLSKVSKIYPLPHMYVIKDLVPDLSNFYAQYKSIEPYLKKKDESQEGKQQYLQSIEDREKLDGLYECILCACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDDFTEERLAKLQDPFSLYRCHTIMNCTRTCPKGLNPGKAIAEIKKMMATYKGKKASV

Target ORF information:

RefSeq Version	NM_001252217.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris succinate dehydrogenase complex iron sulfur subunit B (SDHB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252217.1

ORF Insert Sequence:

ATGGCGGCGG TGGTCGGAGT CTCCTTGAAA CGCCGGTTCC CGGCTGCAGC CCTCGGCGGA 
GCCTGCCTGC AGGCCTGCCG GGGGGCACAG ACAGCGGCAG CCACAGCTCC CCGAATCAAG 
AAATTTGCCA TCTACCGATG GGACCCAGAC AAGACTGGAG ATAAACCGCA TATGCAGACT 
TATGAAATTG ATTTGAATAA ATGTGGTCCT ATGGTGTTGG ATGCTTTAAT CAAGATTAAG 
AATGAAATTG ACTCTACCTT GACCTTCCGA AGATCATGCA GAGAAGGCAT CTGTGGCTCC 
TGTGCCATGA ACATCAACGG AGGCAACACT CTGGCTTGCA CCCGCCGGAT CGACACCAAC 
CTCAGCAAAG TCTCCAAAAT CTACCCTCTG CCGCATATGT ATGTGATAAA GGATCTGGTT 
CCTGACTTGA GCAACTTCTA TGCTCAGTAT AAATCCATTG AACCTTATTT GAAGAAGAAG 
GATGAATCCC AAGAGGGCAA GCAGCAGTAT CTGCAGTCCA TAGAAGATCG TGAGAAACTG 
GACGGGCTGT ACGAGTGTAT CCTCTGTGCT TGCTGCAGCA CCAGCTGCCC TAGCTACTGG 
TGGAATGGAG ACAAATACCT GGGGCCTGCG GTTCTCATGC AGGCCTACCG CTGGATGATC 
GACTCCAGAG ACGACTTCAC TGAGGAGCGC CTGGCCAAGC TGCAGGACCC GTTCTCTCTG 
TACCGCTGCC ACACCATCAT GAACTGCACA AGGACCTGCC CCAAGGGGCT GAATCCAGGC 
AAAGCTATTG CTGAAATCAA GAAAATGATG GCCACCTATA AGGGGAAGAA AGCGTCCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Succinate dehydrogenase subunit D and succinate dehydrogenase subunit B mutation analysis in canine phaeochromocytoma and paraganglioma.
Journal of comparative pathology151(1)25-34(2014 Jul)
Holt DE,Henthorn P,Howell VM,Robinson BG,Benn DE

Housekeeping genes for phylogenetic analysis of eutherian relationships.
Molecular biology and evolution23(8)1493-503(2006 Aug)
Kullberg M,Nilsson MA,Arnason U,Harley EH,Janke A