MAP3K1 cDNA ORF clone, Canis lupus familiaris(dog)

The following MAP3K1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe03116 XM_005617401.2
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe03117 XM_005617402.2
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe03115 XM_535240.6
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe03118 XM_005617403.1
Latest version!
Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
OCe03115 XM_535240.5 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe03116
    Clone ID Related Accession (Same CDS sequence) XM_005617401.2
    Accession Version XM_005617401.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617401.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTCGTG AGATGGAGAA TAAAGAAACT CTCAAGGGGT TGCACAAGAT GGACGATCGC 
    CCAGAAGAAC GAATGATTAG GGAGAAACTG AAGGCAACCT GTATGCCTGC CTGGAAGCAC
    GAGTGGCTGG AAAGGAGAAA CAGGAGAGGC CCTGTGGTGG TAAAACCAAT CCCTACTAAA
    GGAGATGGTT CTGAAATTCA CAATCTGGCT ACTGAGTCTC AAGGAGAAGG CCAGGCAAGC
    ACTGCATCAC CCACTGCTAA AGGCCGACGC AGTCCTTCTC CTGGCAGCTC CCCATCTGGT
    CGCACAGTGA AGTCCGAATC TCCGGGAGTT CGGAGAAAAA GAGTTTCCCC GGTGCCTTTT
    CAGAGTGGGA GAATCACACC ACCTCGAAGA GCCCCTTCAC CAGATGGCTT CTCACCATAT
    AGCCCAGAGG AGACAAACCG CCGTGTAAAC AAAGTTATGA GAGCCAGGCT GTACTTATTG
    CAACAAATAG GACCCAACTC TTTCCTGATT GGAGGAGACA GCCCGGACAA TAAATACCGG
    GTGTTTATTG GGCCTCAGAA CTGCAGCTGT GGGCGTGGAA CATTTTGTAT TCATCTGTTG
    TTTGTTATGC TCCGGGTATT TCAACTGGAA CCTTCAGATC CAATGTTATG GAGAAAAACT
    TTAAAGAATT TTGAGGTTGA AAGTTTGTTC CAGAAATATC ACAGTAGGCG TAGCTCAAGG
    ATCAAAGCTC CATCTCGTAA CACCATCCAG AAGTTTGTTT CACGCATGTC AAATTCTCAT
    ACATTGTCAT CATCTAGTAC TTCTACATCT AGTTCAGAAA ACAGTATAAA AGATGAGGAG
    GAGCAGATGT GTCCTATTTG CTTGTTGGGC ATGCTAGATG AAGAGAGCCT CACGGTGTGT
    GAAGATGGCT GCAGAAACAA GCTGCATCAC CACTGCATGT CAATCTGGGC AGAAGAATGT
    AGAAGAAATA GAGAGCCTTT AATATGTCCC CTTTGTAGGT CTAAGTGGAG ATCTCACGAT
    TTCTACAGCC ATGAATTGTC AAGCCCTGTG GATTCCCCTT CTTCCCTGCG AGCTGCACAG
    CAGCAAACCA TACAACAGCA GCCTTTGGCT GGATCACAAC GAAGGAGTCA GGAGAGCAAT
    TTTAACCTTA CTCATTATGG AACTCAGCAG ATTCCTCCTG CTTATAAAGA TTTAGCTGAG
    CCATGGATTC AGGTATTTGG AATGGAACTT GTTGGCTGCT TATTTTCTAA AAACTGGAAT
    GTAAGAGAGA TGGCTCTCAG GCGTCTTTCA CACGATGTTA GTGGGGCTTT GCTCTTGGCA
    AATGGGGAGA GCACTGGAAA CTCTGGGAGC AACGGTAGAA GCAGTCCGAG TGCTGGAGCC
    AGCAGTGGGT CTTCCCAGAC CAGTATCTCA GGAGACGTGG TAGAGGCATG CTGCAACGTT
    TTGTCAATGG TCTGTGCTGA CCCTGTCTAC AAAGTGTACG TTGCTGCTTT AAAAACATTG
    AGAGCCATGC TAGTATATAC TCCTTGCCAT AGTTTGGCAG AAAGAATCAA ACTGCAGAGA
    CTTCTCCGGC CAGTGGTAGA CACCATCCTA GTCAAGTGTG CAGATGCCAA TAGTCGTACA
    AGTCAGCTGT CTATATCAAC ACTGTTGGAA TTATGCAAAG GCCAAGCAGG AGAGTTGGCA
    GTTGGCAGAG AAATACTTAA AGCTGGATCC ATTGGTATTG GTGGCGTGGA TTACGTCTTA
    AGTTGTATTC TTGGAAACCA AACTGAGTCA AACAACTGGC AAGAACTTCT GGGCCGCCTT
    TGTCTTGTAG ACAGACTGCT GTTGGAATTT CCTGCTGAAT TTTACCCTCA TATTGTCAGT
    ACTGATGTTT CACAAGCTGA GCCTGTTGAA GTCAGGTATA AAAAGCTGCT GTCCCTCTTA
    ACCTTTGCTT TGCAGTCCAT TGATAATTCC CACTCAATGG TTGGTAAACT CTCCCGAAGG
    ATATATTTGA GTTCTGCAAG AATGGTTACT GCAGTACCCC ACGTATTTTC AAAACTATTA
    GAAATGCTGA ATGTTTCCAG TTCCACTCAT TTTACCAGGA TGCGTCGTCG TTTGATGGCT
    ATTGCAGATG AGGTGGAAAT CGCTGAGGCC ATCCATCTGG GCATAGGAGA CACTTTGGAT
    GATCGACATG ACAGCTTTTT GCAGGCATCC GTCCCCACCA GCTATCCAGA AGCCACAGAG
    AACAGCCCCC TTGAGCGTAC AGACCATTTA GAGAAAACTG GAAAAGGACT GTGTGCTACA
    AAACTGAGTG CCAATTCAGA GGACATTACT GACAGACTGG GCAGCATTTC CGTAGGACTT
    CCCAGTTCAA CAATGGAGCA ACCAAAGCCA ATGGTTCAAA CAAAAGGCAG ACCGCACAGT
    CAGTGTTTGA ACTCCTCTCC TTTATCTCAT CATTCCCCGT TAATGTTTCC ACCCTTGTCA
    ACTCCTCCTT CATCTGCCCC ATCTGCACCA CCTGGCACTG GAACAGATGT CTCTAAGCAT
    AGACCTCAGG GATTCACTCC CTGCAAAATA CCTTCTGCAT CTCCTCAAAC ACAGCGCAAG
    TTTTCTCTAC AGTTCCAGAG AAACTGTTCC GAAAACAGAG ACTCAGATAA ACTTTCCCCC
    ATTTTTACTC AATCAAGACC CCTGCCCTCT AGTAACATAC ACAGGCCAAA GCCATCTCGA
    CCTACCCCAG GTAATACAAG TAAACAGGGA GATGCCTCAA AGAATAGCAT GACACTTGAT
    CTGAACAGTA CTTCCAAATG TGATGACAGC TTTGGCAGCA GCAGCAATAG TAGTAATGCT
    CTTATACCCA GTGATGAGAC AGTGTTCACC CCAGTAGAAG AGAAATGCAG GTTAGATGTC
    AACACAGAAC TCAACTCCAG TATTGAGGAC CTTCTTGAAG CATCCATGCC TTCAAGTGAC
    ACGACAGTAA CTTTTAAGTC AGAAGTTGCT GTCCTCTCTC CTGAAAAGGA TGAAAATGAT
    GATACCTACA AAGATGATGT GAATCATAAT CAGAAATGCA AAGAAAAGAT GGAAGCTGAA
    GAAGAAGAAG CTTTAGCAAT TGCCATGGCA ATGTCAGCGT CTCAGGATGC CCTCCCCATA
    GTTCCTCAAC TACAGGTTGA AAATGGAGAA GATATCATCA TTATTCAACA GGATACACCG
    GAAACTCTAC CAGGACATAC CAAAGCAAAA CAACCTTACA GAGAAGACAC TGAATGGCTG
    AAAGGTCAGC AGATAGGCCT TGGAGCATTT TCTTCTTGTT ACCAGGCTCA GGATGTGGGA
    ACTGGAACTT TAATGGCTGT TAAACAGGTG ACATATGTCA GAAATACATC TTCTGAACAA
    GAAGAAGTAG TGGAAGCATT AAGAGAAGAG ATCAGAATGA TGAGCCATTT GAATCATCCC
    AACATCATTA GGATGTTGGG AGCCACATGT GAGAAGAGCA ATTATAATCT CTTCATTGAA
    TGGATGGCAG GGGGATCTGT GGCTCATTTG CTCAGTAAAT ACGGGGCCTT CAAGGAATCA
    GTAGTTATTA ACTATACTGA ACAGTTACTT CGTGGCCTTT CCTATCTCCA TGAAAACCAG
    ATCATTCATA GAGATGTCAA AGGTGCCAAT TTGCTAATTG ACAGCACAGG TCAGAGACTG
    AGAATTGCAG ATTTTGGAGC TGCAGCCAGG TTGGCATCAA AAGGAACTGG TGCAGGAGAG
    TTTCAGGGAC AGTTATTGGG AACAATTGCA TTTATGGCAC CTGAGGTACT GAGAGGTCAG
    CAGTATGGTA GGAGCTGTGA TGTATGGAGT GTTGGCTGTG CTATTATAGA AATGGCTTGT
    GCTAAACCAC CTTGGAATGC AGAAAAACAC TCCAATCATC TTGCTTTGAT ATTTAAGATT
    GCAAGTGCGA CGACTGCTCC ATCAATCCCC TCACATTTGT CTCCTGGTTT ACGAGATGTG
    GCTCTTCGGT GTTTAGAACT TCAACCTCAG GACAGACCCC CGTCAAGAGA GCTACTGAAG
    CATCCGGTCT TCCGTACTAC ATGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617458.1
    CDS88..4134
    Translation

    Target ORF information:

    RefSeq Version XM_005617401.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617401.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGTCGTG AGATGGAGAA TAAAGAAACT CTCAAGGGGT TGCACAAGAT GGACGATCGC 
    CCAGAAGAAC GAATGATTAG GGAGAAACTG AAGGCAACCT GTATGCCTGC CTGGAAGCAC
    GAGTGGCTGG AAAGGAGAAA CAGGAGAGGC CCTGTGGTGG TAAAACCAAT CCCTACTAAA
    GGAGATGGTT CTGAAATTCA CAATCTGGCT ACTGAGTCTC AAGGAGAAGG CCAGGCAAGC
    ACTGCATCAC CCACTGCTAA AGGCCGACGC AGTCCTTCTC CTGGCAGCTC CCCATCTGGT
    CGCACAGTGA AGTCCGAATC TCCGGGAGTT CGGAGAAAAA GAGTTTCCCC GGTGCCTTTT
    CAGAGTGGGA GAATCACACC ACCTCGAAGA GCCCCTTCAC CAGATGGCTT CTCACCATAT
    AGCCCAGAGG AGACAAACCG CCGTGTAAAC AAAGTTATGA GAGCCAGGCT GTACTTATTG
    CAACAAATAG GACCCAACTC TTTCCTGATT GGAGGAGACA GCCCGGACAA TAAATACCGG
    GTGTTTATTG GGCCTCAGAA CTGCAGCTGT GGGCGTGGAA CATTTTGTAT TCATCTGTTG
    TTTGTTATGC TCCGGGTATT TCAACTGGAA CCTTCAGATC CAATGTTATG GAGAAAAACT
    TTAAAGAATT TTGAGGTTGA AAGTTTGTTC CAGAAATATC ACAGTAGGCG TAGCTCAAGG
    ATCAAAGCTC CATCTCGTAA CACCATCCAG AAGTTTGTTT CACGCATGTC AAATTCTCAT
    ACATTGTCAT CATCTAGTAC TTCTACATCT AGTTCAGAAA ACAGTATAAA AGATGAGGAG
    GAGCAGATGT GTCCTATTTG CTTGTTGGGC ATGCTAGATG AAGAGAGCCT CACGGTGTGT
    GAAGATGGCT GCAGAAACAA GCTGCATCAC CACTGCATGT CAATCTGGGC AGAAGAATGT
    AGAAGAAATA GAGAGCCTTT AATATGTCCC CTTTGTAGGT CTAAGTGGAG ATCTCACGAT
    TTCTACAGCC ATGAATTGTC AAGCCCTGTG GATTCCCCTT CTTCCCTGCG AGCTGCACAG
    CAGCAAACCA TACAACAGCA GCCTTTGGCT GGATCACAAC GAAGGAGTCA GGAGAGCAAT
    TTTAACCTTA CTCATTATGG AACTCAGCAG ATTCCTCCTG CTTATAAAGA TTTAGCTGAG
    CCATGGATTC AGGTATTTGG AATGGAACTT GTTGGCTGCT TATTTTCTAA AAACTGGAAT
    GTAAGAGAGA TGGCTCTCAG GCGTCTTTCA CACGATGTTA GTGGGGCTTT GCTCTTGGCA
    AATGGGGAGA GCACTGGAAA CTCTGGGAGC AACGGTAGAA GCAGTCCGAG TGCTGGAGCC
    AGCAGTGGGT CTTCCCAGAC CAGTATCTCA GGAGACGTGG TAGAGGCATG CTGCAACGTT
    TTGTCAATGG TCTGTGCTGA CCCTGTCTAC AAAGTGTACG TTGCTGCTTT AAAAACATTG
    AGAGCCATGC TAGTATATAC TCCTTGCCAT AGTTTGGCAG AAAGAATCAA ACTGCAGAGA
    CTTCTCCGGC CAGTGGTAGA CACCATCCTA GTCAAGTGTG CAGATGCCAA TAGTCGTACA
    AGTCAGCTGT CTATATCAAC ACTGTTGGAA TTATGCAAAG GCCAAGCAGG AGAGTTGGCA
    GTTGGCAGAG AAATACTTAA AGCTGGATCC ATTGGTATTG GTGGCGTGGA TTACGTCTTA
    AGTTGTATTC TTGGAAACCA AACTGAGTCA AACAACTGGC AAGAACTTCT GGGCCGCCTT
    TGTCTTGTAG ACAGACTGCT GTTGGAATTT CCTGCTGAAT TTTACCCTCA TATTGTCAGT
    ACTGATGTTT CACAAGCTGA GCCTGTTGAA GTCAGGTATA AAAAGCTGCT GTCCCTCTTA
    ACCTTTGCTT TGCAGTCCAT TGATAATTCC CACTCAATGG TTGGTAAACT CTCCCGAAGG
    ATATATTTGA GTTCTGCAAG AATGGTTACT GCAGTACCCC ACGTATTTTC AAAACTATTA
    GAAATGCTGA ATGTTTCCAG TTCCACTCAT TTTACCAGGA TGCGTCGTCG TTTGATGGCT
    ATTGCAGATG AGGTGGAAAT CGCTGAGGCC ATCCATCTGG GCATAGGAGA CACTTTGGAT
    GATCGACATG ACAGCTTTTT GCAGGCATCC GTCCCCACCA GCTATCCAGA AGCCACAGAG
    AACAGCCCCC TTGAGCGTAC AGACCATTTA GAGAAAACTG GAAAAGGACT GTGTGCTACA
    AAACTGAGTG CCAATTCAGA GGACATTACT GACAGACTGG GCAGCATTTC CGTAGGACTT
    CCCAGTTCAA CAATGGAGCA ACCAAAGCCA ATGGTTCAAA CAAAAGGCAG ACCGCACAGT
    CAGTGTTTGA ACTCCTCTCC TTTATCTCAT CATTCCCCGT TAATGTTTCC ACCCTTGTCA
    ACTCCTCCTT CATCTGCCCC ATCTGCACCA CCTGGCACTG GAACAGATGT CTCTAAGCAT
    AGACCTCAGG GATTCACTCC CTGCAAAATA CCTTCTGCAT CTCCTCAAAC ACAGCGCAAG
    TTTTCTCTAC AGTTCCAGAG AAACTGTTCC GAAAACAGAG ACTCAGATAA ACTTTCCCCC
    ATTTTTACTC AATCAAGACC CCTGCCCTCT AGTAACATAC ACAGGCCAAA GCCATCTCGA
    CCTACCCCAG GTAATACAAG TAAACAGGGA GATGCCTCAA AGAATAGCAT GACACTTGAT
    CTGAACAGTA CTTCCAAATG TGATGACAGC TTTGGCAGCA GCAGCAATAG TAGTAATGCT
    CTTATACCCA GTGATGAGAC AGTGTTCACC CCAGTAGAAG AGAAATGCAG GTTAGATGTC
    AACACAGAAC TCAACTCCAG TATTGAGGAC CTTCTTGAAG CATCCATGCC TTCAAGTGAC
    ACGACAGTAA CTTTTAAGTC AGAAGTTGCT GTCCTCTCTC CTGAAAAGGA TGAAAATGAT
    GATACCTACA AAGATGATGT GAATCATAAT CAGAAATGCA AAGAAAAGAT GGAAGCTGAA
    GAAGAAGAAG CTTTAGCAAT TGCCATGGCA ATGTCAGCGT CTCAGGATGC CCTCCCCATA
    GTTCCTCAAC TACAGGTTGA AAATGGAGAA GATATCATCA TTATTCAACA GGATACACCG
    GAAACTCTAC CAGGACATAC CAAAGCAAAA CAACCTTACA GAGAAGACAC TGAATGGCTG
    AAAGGTCAGC AGATAGGCCT TGGAGCATTT TCTTCTTGTT ACCAGGCTCA GGATGTGGGA
    ACTGGAACTT TAATGGCTGT TAAACAGGTG ACATATGTCA GAAATACATC TTCTGAACAA
    GAAGAAGTAG TGGAAGCATT AAGAGAAGAG ATCAGAATGA TGAGCCATTT GAATCATCCC
    AACATCATTA GGATGTTGGG AGCCACATGT GAGAAGAGCA ATTATAATCT CTTCATTGAA
    TGGATGGCAG GGGGATCTGT GGCTCATTTG CTCAGTAAAT ACGGGGCCTT CAAGGAATCA
    GTAGTTATTA ACTATACTGA ACAGTTACTT CGTGGCCTTT CCTATCTCCA TGAAAACCAG
    ATCATTCATA GAGATGTCAA AGGTGCCAAT TTGCTAATTG ACAGCACAGG TCAGAGACTG
    AGAATTGCAG ATTTTGGAGC TGCAGCCAGG TTGGCATCAA AAGGAACTGG TGCAGGAGAG
    TTTCAGGGAC AGTTATTGGG AACAATTGCA TTTATGGCAC CTGAGGTACT GAGAGGTCAG
    CAGTATGGTA GGAGCTGTGA TGTATGGAGT GTTGGCTGTG CTATTATAGA AATGGCTTGT
    GCTAAACCAC CTTGGAATGC AGAAAAACAC TCCAATCATC TTGCTTTGAT ATTTAAGATT
    GCAAGTGCGA CGACTGCTCC ATCAATCCCC TCACATTTGT CTCCTGGTTT ACGAGATGTG
    GCTCTTCGGT GTTTAGAACT TCAACCTCAG GACAGACCCC CGTCAAGAGA GCTACTGAAG
    CATCCGGTCT TCCGTACTAC ATGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03117
    Clone ID Related Accession (Same CDS sequence) XM_005617402.2
    Accession Version XM_005617402.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617402.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGAATA AAGAAACTCT CAAGGGGTTG CACAAGATGG ACGATCGCCC AGAAGAACGA 
    ATGATTAGGG AGAAACTGAA GGCAACCTGT ATGCCTGCCT GGAAGCACGA GTGGCTGGAA
    AGGAGAAACA GGAGAGGCCC TGTGGTGGTA AAACCAATCC CTACTAAAGG AGATGGTTCT
    GAAATTCACA ATCTGGCTAC TGAGTCTCAA GGAGAAGGCC AGGCAAGCAC TGCATCACCC
    ACTGCTAAAG GCCGACGCAG TCCTTCTCCT GGCAGCTCCC CATCTGGTCG CACAGTGAAG
    TCCGAATCTC CGGGAGTTCG GAGAAAAAGA GTTTCCCCGG TGCCTTTTCA GAGTGGGAGA
    ATCACACCAC CTCGAAGAGC CCCTTCACCA GATGGCTTCT CACCATATAG CCCAGAGGAG
    ACAAACCGCC GTGTAAACAA AGTTATGAGA GCCAGGCTGT ACTTATTGCA ACAAATAGGA
    CCCAACTCTT TCCTGATTGG AGGAGACAGC CCGGACAATA AATACCGGGT GTTTATTGGG
    CCTCAGAACT GCAGCTGTGG GCGTGGAACA TTTTGTATTC ATCTGTTGTT TGTTATGCTC
    CGGGTATTTC AACTGGAACC TTCAGATCCA ATGTTATGGA GAAAAACTTT AAAGAATTTT
    GAGGTTGAAA GTTTGTTCCA GAAATATCAC AGTAGGCGTA GCTCAAGGAT CAAAGCTCCA
    TCTCGTAACA CCATCCAGAA GTTTGTTTCA CGCATGTCAA ATTCTCATAC ATTGTCATCA
    TCTAGTACTT CTACATCTAG TTCAGAAAAC AGTATAAAAG ATGAGGAGGA GCAGATGTGT
    CCTATTTGCT TGTTGGGCAT GCTAGATGAA GAGAGCCTCA CGGTGTGTGA AGATGGCTGC
    AGAAACAAGC TGCATCACCA CTGCATGTCA ATCTGGGCAG AAGAATGTAG AAGAAATAGA
    GAGCCTTTAA TATGTCCCCT TTGTAGGTCT AAGTGGAGAT CTCACGATTT CTACAGCCAT
    GAATTGTCAA GCCCTGTGGA TTCCCCTTCT TCCCTGCGAG CTGCACAGCA GCAAACCATA
    CAACAGCAGC CTTTGGCTGG ATCACAACGA AGGAGTCAGG AGAGCAATTT TAACCTTACT
    CATTATGGAA CTCAGCAGAT TCCTCCTGCT TATAAAGATT TAGCTGAGCC ATGGATTCAG
    GTATTTGGAA TGGAACTTGT TGGCTGCTTA TTTTCTAAAA ACTGGAATGT AAGAGAGATG
    GCTCTCAGGC GTCTTTCACA CGATGTTAGT GGGGCTTTGC TCTTGGCAAA TGGGGAGAGC
    ACTGGAAACT CTGGGAGCAA CGGTAGAAGC AGTCCGAGTG CTGGAGCCAG CAGTGGGTCT
    TCCCAGACCA GTATCTCAGG AGACGTGGTA GAGGCATGCT GCAACGTTTT GTCAATGGTC
    TGTGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACATTGAG AGCCATGCTA
    GTATATACTC CTTGCCATAG TTTGGCAGAA AGAATCAAAC TGCAGAGACT TCTCCGGCCA
    GTGGTAGACA CCATCCTAGT CAAGTGTGCA GATGCCAATA GTCGTACAAG TCAGCTGTCT
    ATATCAACAC TGTTGGAATT ATGCAAAGGC CAAGCAGGAG AGTTGGCAGT TGGCAGAGAA
    ATACTTAAAG CTGGATCCAT TGGTATTGGT GGCGTGGATT ACGTCTTAAG TTGTATTCTT
    GGAAACCAAA CTGAGTCAAA CAACTGGCAA GAACTTCTGG GCCGCCTTTG TCTTGTAGAC
    AGACTGCTGT TGGAATTTCC TGCTGAATTT TACCCTCATA TTGTCAGTAC TGATGTTTCA
    CAAGCTGAGC CTGTTGAAGT CAGGTATAAA AAGCTGCTGT CCCTCTTAAC CTTTGCTTTG
    CAGTCCATTG ATAATTCCCA CTCAATGGTT GGTAAACTCT CCCGAAGGAT ATATTTGAGT
    TCTGCAAGAA TGGTTACTGC AGTACCCCAC GTATTTTCAA AACTATTAGA AATGCTGAAT
    GTTTCCAGTT CCACTCATTT TACCAGGATG CGTCGTCGTT TGATGGCTAT TGCAGATGAG
    GTGGAAATCG CTGAGGCCAT CCATCTGGGC ATAGGAGACA CTTTGGATGA TCGACATGAC
    AGCTTTTTGC AGGCATCCGT CCCCACCAGC TATCCAGAAG CCACAGAGAA CAGCCCCCTT
    GAGCGTACAG ACCATTTAGA GAAAACTGGA AAAGGACTGT GTGCTACAAA ACTGAGTGCC
    AATTCAGAGG ACATTACTGA CAGACTGGGC AGCATTTCCG TAGGACTTCC CAGTTCAACA
    ATGGAGCAAC CAAAGCCAAT GGTTCAAACA AAAGGCAGAC CGCACAGTCA GTGTTTGAAC
    TCCTCTCCTT TATCTCATCA TTCCCCGTTA ATGTTTCCAC CCTTGTCAAC TCCTCCTTCA
    TCTGCCCCAT CTGCACCACC TGGCACTGGA ACAGATGTCT CTAAGCATAG ACCTCAGGGA
    TTCACTCCCT GCAAAATACC TTCTGCATCT CCTCAAACAC AGCGCAAGTT TTCTCTACAG
    TTCCAGAGAA ACTGTTCCGA AAACAGAGAC TCAGATAAAC TTTCCCCCAT TTTTACTCAA
    TCAAGACCCC TGCCCTCTAG TAACATACAC AGGCCAAAGC CATCTCGACC TACCCCAGGT
    AATACAAGTA AACAGGGAGA TGCCTCAAAG AATAGCATGA CACTTGATCT GAACAGTACT
    TCCAAATGTG ATGACAGCTT TGGCAGCAGC AGCAATAGTA GTAATGCTCT TATACCCAGT
    GATGAGACAG TGTTCACCCC AGTAGAAGAG AAATGCAGGT TAGATGTCAA CACAGAACTC
    AACTCCAGTA TTGAGGACCT TCTTGAAGCA TCCATGCCTT CAAGTGACAC GACAGTAACT
    TTTAAGTCAG AAGTTGCTGT CCTCTCTCCT GAAAAGGATG AAAATGATGA TACCTACAAA
    GATGATGTGA ATCATAATCA GAAATGCAAA GAAAAGATGG AAGCTGAAGA AGAAGAAGCT
    TTAGCAATTG CCATGGCAAT GTCAGCGTCT CAGGATGCCC TCCCCATAGT TCCTCAACTA
    CAGGTTGAAA ATGGAGAAGA TATCATCATT ATTCAACAGG ATACACCGGA AACTCTACCA
    GGACATACCA AAGCAAAACA ACCTTACAGA GAAGACACTG AATGGCTGAA AGGTCAGCAG
    ATAGGCCTTG GAGCATTTTC TTCTTGTTAC CAGGCTCAGG ATGTGGGAAC TGGAACTTTA
    ATGGCTGTTA AACAGGTGAC ATATGTCAGA AATACATCTT CTGAACAAGA AGAAGTAGTG
    GAAGCATTAA GAGAAGAGAT CAGAATGATG AGCCATTTGA ATCATCCCAA CATCATTAGG
    ATGTTGGGAG CCACATGTGA GAAGAGCAAT TATAATCTCT TCATTGAATG GATGGCAGGG
    GGATCTGTGG CTCATTTGCT CAGTAAATAC GGGGCCTTCA AGGAATCAGT AGTTATTAAC
    TATACTGAAC AGTTACTTCG TGGCCTTTCC TATCTCCATG AAAACCAGAT CATTCATAGA
    GATGTCAAAG GTGCCAATTT GCTAATTGAC AGCACAGGTC AGAGACTGAG AATTGCAGAT
    TTTGGAGCTG CAGCCAGGTT GGCATCAAAA GGAACTGGTG CAGGAGAGTT TCAGGGACAG
    TTATTGGGAA CAATTGCATT TATGGCACCT GAGGTACTGA GAGGTCAGCA GTATGGTAGG
    AGCTGTGATG TATGGAGTGT TGGCTGTGCT ATTATAGAAA TGGCTTGTGC TAAACCACCT
    TGGAATGCAG AAAAACACTC CAATCATCTT GCTTTGATAT TTAAGATTGC AAGTGCGACG
    ACTGCTCCAT CAATCCCCTC ACATTTGTCT CCTGGTTTAC GAGATGTGGC TCTTCGGTGT
    TTAGAACTTC AACCTCAGGA CAGACCCCCG TCAAGAGAGC TACTGAAGCA TCCGGTCTTC
    CGTACTACAT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617459.1
    CDS25..4059
    Translation

    Target ORF information:

    RefSeq Version XM_005617402.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617402.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGAATA AAGAAACTCT CAAGGGGTTG CACAAGATGG ACGATCGCCC AGAAGAACGA 
    ATGATTAGGG AGAAACTGAA GGCAACCTGT ATGCCTGCCT GGAAGCACGA GTGGCTGGAA
    AGGAGAAACA GGAGAGGCCC TGTGGTGGTA AAACCAATCC CTACTAAAGG AGATGGTTCT
    GAAATTCACA ATCTGGCTAC TGAGTCTCAA GGAGAAGGCC AGGCAAGCAC TGCATCACCC
    ACTGCTAAAG GCCGACGCAG TCCTTCTCCT GGCAGCTCCC CATCTGGTCG CACAGTGAAG
    TCCGAATCTC CGGGAGTTCG GAGAAAAAGA GTTTCCCCGG TGCCTTTTCA GAGTGGGAGA
    ATCACACCAC CTCGAAGAGC CCCTTCACCA GATGGCTTCT CACCATATAG CCCAGAGGAG
    ACAAACCGCC GTGTAAACAA AGTTATGAGA GCCAGGCTGT ACTTATTGCA ACAAATAGGA
    CCCAACTCTT TCCTGATTGG AGGAGACAGC CCGGACAATA AATACCGGGT GTTTATTGGG
    CCTCAGAACT GCAGCTGTGG GCGTGGAACA TTTTGTATTC ATCTGTTGTT TGTTATGCTC
    CGGGTATTTC AACTGGAACC TTCAGATCCA ATGTTATGGA GAAAAACTTT AAAGAATTTT
    GAGGTTGAAA GTTTGTTCCA GAAATATCAC AGTAGGCGTA GCTCAAGGAT CAAAGCTCCA
    TCTCGTAACA CCATCCAGAA GTTTGTTTCA CGCATGTCAA ATTCTCATAC ATTGTCATCA
    TCTAGTACTT CTACATCTAG TTCAGAAAAC AGTATAAAAG ATGAGGAGGA GCAGATGTGT
    CCTATTTGCT TGTTGGGCAT GCTAGATGAA GAGAGCCTCA CGGTGTGTGA AGATGGCTGC
    AGAAACAAGC TGCATCACCA CTGCATGTCA ATCTGGGCAG AAGAATGTAG AAGAAATAGA
    GAGCCTTTAA TATGTCCCCT TTGTAGGTCT AAGTGGAGAT CTCACGATTT CTACAGCCAT
    GAATTGTCAA GCCCTGTGGA TTCCCCTTCT TCCCTGCGAG CTGCACAGCA GCAAACCATA
    CAACAGCAGC CTTTGGCTGG ATCACAACGA AGGAGTCAGG AGAGCAATTT TAACCTTACT
    CATTATGGAA CTCAGCAGAT TCCTCCTGCT TATAAAGATT TAGCTGAGCC ATGGATTCAG
    GTATTTGGAA TGGAACTTGT TGGCTGCTTA TTTTCTAAAA ACTGGAATGT AAGAGAGATG
    GCTCTCAGGC GTCTTTCACA CGATGTTAGT GGGGCTTTGC TCTTGGCAAA TGGGGAGAGC
    ACTGGAAACT CTGGGAGCAA CGGTAGAAGC AGTCCGAGTG CTGGAGCCAG CAGTGGGTCT
    TCCCAGACCA GTATCTCAGG AGACGTGGTA GAGGCATGCT GCAACGTTTT GTCAATGGTC
    TGTGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACATTGAG AGCCATGCTA
    GTATATACTC CTTGCCATAG TTTGGCAGAA AGAATCAAAC TGCAGAGACT TCTCCGGCCA
    GTGGTAGACA CCATCCTAGT CAAGTGTGCA GATGCCAATA GTCGTACAAG TCAGCTGTCT
    ATATCAACAC TGTTGGAATT ATGCAAAGGC CAAGCAGGAG AGTTGGCAGT TGGCAGAGAA
    ATACTTAAAG CTGGATCCAT TGGTATTGGT GGCGTGGATT ACGTCTTAAG TTGTATTCTT
    GGAAACCAAA CTGAGTCAAA CAACTGGCAA GAACTTCTGG GCCGCCTTTG TCTTGTAGAC
    AGACTGCTGT TGGAATTTCC TGCTGAATTT TACCCTCATA TTGTCAGTAC TGATGTTTCA
    CAAGCTGAGC CTGTTGAAGT CAGGTATAAA AAGCTGCTGT CCCTCTTAAC CTTTGCTTTG
    CAGTCCATTG ATAATTCCCA CTCAATGGTT GGTAAACTCT CCCGAAGGAT ATATTTGAGT
    TCTGCAAGAA TGGTTACTGC AGTACCCCAC GTATTTTCAA AACTATTAGA AATGCTGAAT
    GTTTCCAGTT CCACTCATTT TACCAGGATG CGTCGTCGTT TGATGGCTAT TGCAGATGAG
    GTGGAAATCG CTGAGGCCAT CCATCTGGGC ATAGGAGACA CTTTGGATGA TCGACATGAC
    AGCTTTTTGC AGGCATCCGT CCCCACCAGC TATCCAGAAG CCACAGAGAA CAGCCCCCTT
    GAGCGTACAG ACCATTTAGA GAAAACTGGA AAAGGACTGT GTGCTACAAA ACTGAGTGCC
    AATTCAGAGG ACATTACTGA CAGACTGGGC AGCATTTCCG TAGGACTTCC CAGTTCAACA
    ATGGAGCAAC CAAAGCCAAT GGTTCAAACA AAAGGCAGAC CGCACAGTCA GTGTTTGAAC
    TCCTCTCCTT TATCTCATCA TTCCCCGTTA ATGTTTCCAC CCTTGTCAAC TCCTCCTTCA
    TCTGCCCCAT CTGCACCACC TGGCACTGGA ACAGATGTCT CTAAGCATAG ACCTCAGGGA
    TTCACTCCCT GCAAAATACC TTCTGCATCT CCTCAAACAC AGCGCAAGTT TTCTCTACAG
    TTCCAGAGAA ACTGTTCCGA AAACAGAGAC TCAGATAAAC TTTCCCCCAT TTTTACTCAA
    TCAAGACCCC TGCCCTCTAG TAACATACAC AGGCCAAAGC CATCTCGACC TACCCCAGGT
    AATACAAGTA AACAGGGAGA TGCCTCAAAG AATAGCATGA CACTTGATCT GAACAGTACT
    TCCAAATGTG ATGACAGCTT TGGCAGCAGC AGCAATAGTA GTAATGCTCT TATACCCAGT
    GATGAGACAG TGTTCACCCC AGTAGAAGAG AAATGCAGGT TAGATGTCAA CACAGAACTC
    AACTCCAGTA TTGAGGACCT TCTTGAAGCA TCCATGCCTT CAAGTGACAC GACAGTAACT
    TTTAAGTCAG AAGTTGCTGT CCTCTCTCCT GAAAAGGATG AAAATGATGA TACCTACAAA
    GATGATGTGA ATCATAATCA GAAATGCAAA GAAAAGATGG AAGCTGAAGA AGAAGAAGCT
    TTAGCAATTG CCATGGCAAT GTCAGCGTCT CAGGATGCCC TCCCCATAGT TCCTCAACTA
    CAGGTTGAAA ATGGAGAAGA TATCATCATT ATTCAACAGG ATACACCGGA AACTCTACCA
    GGACATACCA AAGCAAAACA ACCTTACAGA GAAGACACTG AATGGCTGAA AGGTCAGCAG
    ATAGGCCTTG GAGCATTTTC TTCTTGTTAC CAGGCTCAGG ATGTGGGAAC TGGAACTTTA
    ATGGCTGTTA AACAGGTGAC ATATGTCAGA AATACATCTT CTGAACAAGA AGAAGTAGTG
    GAAGCATTAA GAGAAGAGAT CAGAATGATG AGCCATTTGA ATCATCCCAA CATCATTAGG
    ATGTTGGGAG CCACATGTGA GAAGAGCAAT TATAATCTCT TCATTGAATG GATGGCAGGG
    GGATCTGTGG CTCATTTGCT CAGTAAATAC GGGGCCTTCA AGGAATCAGT AGTTATTAAC
    TATACTGAAC AGTTACTTCG TGGCCTTTCC TATCTCCATG AAAACCAGAT CATTCATAGA
    GATGTCAAAG GTGCCAATTT GCTAATTGAC AGCACAGGTC AGAGACTGAG AATTGCAGAT
    TTTGGAGCTG CAGCCAGGTT GGCATCAAAA GGAACTGGTG CAGGAGAGTT TCAGGGACAG
    TTATTGGGAA CAATTGCATT TATGGCACCT GAGGTACTGA GAGGTCAGCA GTATGGTAGG
    AGCTGTGATG TATGGAGTGT TGGCTGTGCT ATTATAGAAA TGGCTTGTGC TAAACCACCT
    TGGAATGCAG AAAAACACTC CAATCATCTT GCTTTGATAT TTAAGATTGC AAGTGCGACG
    ACTGCTCCAT CAATCCCCTC ACATTTGTCT CCTGGTTTAC GAGATGTGGC TCTTCGGTGT
    TTAGAACTTC AACCTCAGGA CAGACCCCCG TCAAGAGAGC TACTGAAGCA TCCGGTCTTC
    CGTACTACAT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03115
    Clone ID Related Accession (Same CDS sequence) XM_535240.5 , XM_535240.6
    Accession Version XM_535240.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_535240.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGGGGCGGCG 
    AGCCCCGAGG CGGGCGGCGG CGGAGCGGCT CTCAAGGCGA GCAGCGCGCC CGCGGCAGCC
    GCGGGGCTGC TGCGGGAGGG GGGCAGCGGG GGCCGCGAGC GGGCTGACTG GCGGCGGCGG
    CAGCTGCGCA AAGTGCGGAG CGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC
    GCCGCCTCGC CGCCGTCCTC CTCCCCTTCC CCGTCGCCGG AGCCCTCGGA CGCCGCGGCG
    GGCGGGAGCG CCTTCCAGCC TCTGGCGGCG CCGCAGCCGC ACGGAGCCGC GAGCCGTGGC
    GGCGCCCGCC CTCCGGAGCC CGCGGCCGCC CCGGACAGCG GCGCCCCGAG CCCCGCGGGG
    GCCGAGCCCG GGGAGAAGCG GACGCCCGCC GCCGAGCCGC CTCCTGCAGC GGCCCCCGCC
    GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAGGGGTTGC ACAAGATGGA CGATCGCCCA
    GAAGAACGAA TGATTAGGGA GAAACTGAAG GCAACCTGTA TGCCTGCCTG GAAGCACGAG
    TGGCTGGAAA GGAGAAACAG GAGAGGCCCT GTGGTGGTAA AACCAATCCC TACTAAAGGA
    GATGGTTCTG AAATTCACAA TCTGGCTACT GAGTCTCAAG GAGAAGGCCA GGCAAGCACT
    GCATCACCCA CTGCTAAAGG CCGACGCAGT CCTTCTCCTG GCAGCTCCCC ATCTGGTCGC
    ACAGTGAAGT CCGAATCTCC GGGAGTTCGG AGAAAAAGAG TTTCCCCGGT GCCTTTTCAG
    AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC
    CCAGAGGAGA CAAACCGCCG TGTAAACAAA GTTATGAGAG CCAGGCTGTA CTTATTGCAA
    CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGACAGCC CGGACAATAA ATACCGGGTG
    TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTGTTT
    GTTATGCTCC GGGTATTTCA ACTGGAACCT TCAGATCCAA TGTTATGGAG AAAAACTTTA
    AAGAATTTTG AGGTTGAAAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC
    AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA
    TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAGGAGGAG
    CAGATGTGTC CTATTTGCTT GTTGGGCATG CTAGATGAAG AGAGCCTCAC GGTGTGTGAA
    GATGGCTGCA GAAACAAGCT GCATCACCAC TGCATGTCAA TCTGGGCAGA AGAATGTAGA
    AGAAATAGAG AGCCTTTAAT ATGTCCCCTT TGTAGGTCTA AGTGGAGATC TCACGATTTC
    TACAGCCATG AATTGTCAAG CCCTGTGGAT TCCCCTTCTT CCCTGCGAGC TGCACAGCAG
    CAAACCATAC AACAGCAGCC TTTGGCTGGA TCACAACGAA GGAGTCAGGA GAGCAATTTT
    AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA
    TGGATTCAGG TATTTGGAAT GGAACTTGTT GGCTGCTTAT TTTCTAAAAA CTGGAATGTA
    AGAGAGATGG CTCTCAGGCG TCTTTCACAC GATGTTAGTG GGGCTTTGCT CTTGGCAAAT
    GGGGAGAGCA CTGGAAACTC TGGGAGCAAC GGTAGAAGCA GTCCGAGTGC TGGAGCCAGC
    AGTGGGTCTT CCCAGACCAG TATCTCAGGA GACGTGGTAG AGGCATGCTG CAACGTTTTG
    TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACATTGAGA
    GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT
    CTCCGGCCAG TGGTAGACAC CATCCTAGTC AAGTGTGCAG ATGCCAATAG TCGTACAAGT
    CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGCC AAGCAGGAGA GTTGGCAGTT
    GGCAGAGAAA TACTTAAAGC TGGATCCATT GGTATTGGTG GCGTGGATTA CGTCTTAAGT
    TGTATTCTTG GAAACCAAAC TGAGTCAAAC AACTGGCAAG AACTTCTGGG CCGCCTTTGT
    CTTGTAGACA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACT
    GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAAA AGCTGCTGTC CCTCTTAACC
    TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCAATGGTTG GTAAACTCTC CCGAAGGATA
    TATTTGAGTT CTGCAAGAAT GGTTACTGCA GTACCCCACG TATTTTCAAA ACTATTAGAA
    ATGCTGAATG TTTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT
    GCAGATGAGG TGGAAATCGC TGAGGCCATC CATCTGGGCA TAGGAGACAC TTTGGATGAT
    CGACATGACA GCTTTTTGCA GGCATCCGTC CCCACCAGCT ATCCAGAAGC CACAGAGAAC
    AGCCCCCTTG AGCGTACAGA CCATTTAGAG AAAACTGGAA AAGGACTGTG TGCTACAAAA
    CTGAGTGCCA ATTCAGAGGA CATTACTGAC AGACTGGGCA GCATTTCCGT AGGACTTCCC
    AGTTCAACAA TGGAGCAACC AAAGCCAATG GTTCAAACAA AAGGCAGACC GCACAGTCAG
    TGTTTGAACT CCTCTCCTTT ATCTCATCAT TCCCCGTTAA TGTTTCCACC CTTGTCAACT
    CCTCCTTCAT CTGCCCCATC TGCACCACCT GGCACTGGAA CAGATGTCTC TAAGCATAGA
    CCTCAGGGAT TCACTCCCTG CAAAATACCT TCTGCATCTC CTCAAACACA GCGCAAGTTT
    TCTCTACAGT TCCAGAGAAA CTGTTCCGAA AACAGAGACT CAGATAAACT TTCCCCCATT
    TTTACTCAAT CAAGACCCCT GCCCTCTAGT AACATACACA GGCCAAAGCC ATCTCGACCT
    ACCCCAGGTA ATACAAGTAA ACAGGGAGAT GCCTCAAAGA ATAGCATGAC ACTTGATCTG
    AACAGTACTT CCAAATGTGA TGACAGCTTT GGCAGCAGCA GCAATAGTAG TAATGCTCTT
    ATACCCAGTG ATGAGACAGT GTTCACCCCA GTAGAAGAGA AATGCAGGTT AGATGTCAAC
    ACAGAACTCA ACTCCAGTAT TGAGGACCTT CTTGAAGCAT CCATGCCTTC AAGTGACACG
    ACAGTAACTT TTAAGTCAGA AGTTGCTGTC CTCTCTCCTG AAAAGGATGA AAATGATGAT
    ACCTACAAAG ATGATGTGAA TCATAATCAG AAATGCAAAG AAAAGATGGA AGCTGAAGAA
    GAAGAAGCTT TAGCAATTGC CATGGCAATG TCAGCGTCTC AGGATGCCCT CCCCATAGTT
    CCTCAACTAC AGGTTGAAAA TGGAGAAGAT ATCATCATTA TTCAACAGGA TACACCGGAA
    ACTCTACCAG GACATACCAA AGCAAAACAA CCTTACAGAG AAGACACTGA ATGGCTGAAA
    GGTCAGCAGA TAGGCCTTGG AGCATTTTCT TCTTGTTACC AGGCTCAGGA TGTGGGAACT
    GGAACTTTAA TGGCTGTTAA ACAGGTGACA TATGTCAGAA ATACATCTTC TGAACAAGAA
    GAAGTAGTGG AAGCATTAAG AGAAGAGATC AGAATGATGA GCCATTTGAA TCATCCCAAC
    ATCATTAGGA TGTTGGGAGC CACATGTGAG AAGAGCAATT ATAATCTCTT CATTGAATGG
    ATGGCAGGGG GATCTGTGGC TCATTTGCTC AGTAAATACG GGGCCTTCAA GGAATCAGTA
    GTTATTAACT ATACTGAACA GTTACTTCGT GGCCTTTCCT ATCTCCATGA AAACCAGATC
    ATTCATAGAG ATGTCAAAGG TGCCAATTTG CTAATTGACA GCACAGGTCA GAGACTGAGA
    ATTGCAGATT TTGGAGCTGC AGCCAGGTTG GCATCAAAAG GAACTGGTGC AGGAGAGTTT
    CAGGGACAGT TATTGGGAAC AATTGCATTT ATGGCACCTG AGGTACTGAG AGGTCAGCAG
    TATGGTAGGA GCTGTGATGT ATGGAGTGTT GGCTGTGCTA TTATAGAAAT GGCTTGTGCT
    AAACCACCTT GGAATGCAGA AAAACACTCC AATCATCTTG CTTTGATATT TAAGATTGCA
    AGTGCGACGA CTGCTCCATC AATCCCCTCA CATTTGTCTC CTGGTTTACG AGATGTGGCT
    CTTCGGTGTT TAGAACTTCA ACCTCAGGAC AGACCCCCGT CAAGAGAGCT ACTGAAGCAT
    CCGGTCTTCC GTACTACATG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_535240.4
    CDS13..4536
    Translation

    Target ORF information:

    RefSeq Version XM_535240.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_535240.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGGGGCGGCG 
    AGCCCCGAGG CGGGCGGCGG CGGAGCGGCT CTCAAGGCGA GCAGCGCGCC CGCGGCAGCC
    GCGGGGCTGC TGCGGGAGGG GGGCAGCGGG GGCCGCGAGC GGGCTGACTG GCGGCGGCGG
    CAGCTGCGCA AAGTGCGGAG CGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC
    GCCGCCTCGC CGCCGTCCTC CTCCCCTTCC CCGTCGCCGG AGCCCTCGGA CGCCGCGGCG
    GGCGGGAGCG CCTTCCAGCC TCTGGCGGCG CCGCAGCCGC ACGGAGCCGC GAGCCGTGGC
    GGCGCCCGCC CTCCGGAGCC CGCGGCCGCC CCGGACAGCG GCGCCCCGAG CCCCGCGGGG
    GCCGAGCCCG GGGAGAAGCG GACGCCCGCC GCCGAGCCGC CTCCTGCAGC GGCCCCCGCC
    GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAGGGGTTGC ACAAGATGGA CGATCGCCCA
    GAAGAACGAA TGATTAGGGA GAAACTGAAG GCAACCTGTA TGCCTGCCTG GAAGCACGAG
    TGGCTGGAAA GGAGAAACAG GAGAGGCCCT GTGGTGGTAA AACCAATCCC TACTAAAGGA
    GATGGTTCTG AAATTCACAA TCTGGCTACT GAGTCTCAAG GAGAAGGCCA GGCAAGCACT
    GCATCACCCA CTGCTAAAGG CCGACGCAGT CCTTCTCCTG GCAGCTCCCC ATCTGGTCGC
    ACAGTGAAGT CCGAATCTCC GGGAGTTCGG AGAAAAAGAG TTTCCCCGGT GCCTTTTCAG
    AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC
    CCAGAGGAGA CAAACCGCCG TGTAAACAAA GTTATGAGAG CCAGGCTGTA CTTATTGCAA
    CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGACAGCC CGGACAATAA ATACCGGGTG
    TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTGTTT
    GTTATGCTCC GGGTATTTCA ACTGGAACCT TCAGATCCAA TGTTATGGAG AAAAACTTTA
    AAGAATTTTG AGGTTGAAAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC
    AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA
    TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAGGAGGAG
    CAGATGTGTC CTATTTGCTT GTTGGGCATG CTAGATGAAG AGAGCCTCAC GGTGTGTGAA
    GATGGCTGCA GAAACAAGCT GCATCACCAC TGCATGTCAA TCTGGGCAGA AGAATGTAGA
    AGAAATAGAG AGCCTTTAAT ATGTCCCCTT TGTAGGTCTA AGTGGAGATC TCACGATTTC
    TACAGCCATG AATTGTCAAG CCCTGTGGAT TCCCCTTCTT CCCTGCGAGC TGCACAGCAG
    CAAACCATAC AACAGCAGCC TTTGGCTGGA TCACAACGAA GGAGTCAGGA GAGCAATTTT
    AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA
    TGGATTCAGG TATTTGGAAT GGAACTTGTT GGCTGCTTAT TTTCTAAAAA CTGGAATGTA
    AGAGAGATGG CTCTCAGGCG TCTTTCACAC GATGTTAGTG GGGCTTTGCT CTTGGCAAAT
    GGGGAGAGCA CTGGAAACTC TGGGAGCAAC GGTAGAAGCA GTCCGAGTGC TGGAGCCAGC
    AGTGGGTCTT CCCAGACCAG TATCTCAGGA GACGTGGTAG AGGCATGCTG CAACGTTTTG
    TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACATTGAGA
    GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT
    CTCCGGCCAG TGGTAGACAC CATCCTAGTC AAGTGTGCAG ATGCCAATAG TCGTACAAGT
    CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGCC AAGCAGGAGA GTTGGCAGTT
    GGCAGAGAAA TACTTAAAGC TGGATCCATT GGTATTGGTG GCGTGGATTA CGTCTTAAGT
    TGTATTCTTG GAAACCAAAC TGAGTCAAAC AACTGGCAAG AACTTCTGGG CCGCCTTTGT
    CTTGTAGACA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACT
    GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAAA AGCTGCTGTC CCTCTTAACC
    TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCAATGGTTG GTAAACTCTC CCGAAGGATA
    TATTTGAGTT CTGCAAGAAT GGTTACTGCA GTACCCCACG TATTTTCAAA ACTATTAGAA
    ATGCTGAATG TTTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT
    GCAGATGAGG TGGAAATCGC TGAGGCCATC CATCTGGGCA TAGGAGACAC TTTGGATGAT
    CGACATGACA GCTTTTTGCA GGCATCCGTC CCCACCAGCT ATCCAGAAGC CACAGAGAAC
    AGCCCCCTTG AGCGTACAGA CCATTTAGAG AAAACTGGAA AAGGACTGTG TGCTACAAAA
    CTGAGTGCCA ATTCAGAGGA CATTACTGAC AGACTGGGCA GCATTTCCGT AGGACTTCCC
    AGTTCAACAA TGGAGCAACC AAAGCCAATG GTTCAAACAA AAGGCAGACC GCACAGTCAG
    TGTTTGAACT CCTCTCCTTT ATCTCATCAT TCCCCGTTAA TGTTTCCACC CTTGTCAACT
    CCTCCTTCAT CTGCCCCATC TGCACCACCT GGCACTGGAA CAGATGTCTC TAAGCATAGA
    CCTCAGGGAT TCACTCCCTG CAAAATACCT TCTGCATCTC CTCAAACACA GCGCAAGTTT
    TCTCTACAGT TCCAGAGAAA CTGTTCCGAA AACAGAGACT CAGATAAACT TTCCCCCATT
    TTTACTCAAT CAAGACCCCT GCCCTCTAGT AACATACACA GGCCAAAGCC ATCTCGACCT
    ACCCCAGGTA ATACAAGTAA ACAGGGAGAT GCCTCAAAGA ATAGCATGAC ACTTGATCTG
    AACAGTACTT CCAAATGTGA TGACAGCTTT GGCAGCAGCA GCAATAGTAG TAATGCTCTT
    ATACCCAGTG ATGAGACAGT GTTCACCCCA GTAGAAGAGA AATGCAGGTT AGATGTCAAC
    ACAGAACTCA ACTCCAGTAT TGAGGACCTT CTTGAAGCAT CCATGCCTTC AAGTGACACG
    ACAGTAACTT TTAAGTCAGA AGTTGCTGTC CTCTCTCCTG AAAAGGATGA AAATGATGAT
    ACCTACAAAG ATGATGTGAA TCATAATCAG AAATGCAAAG AAAAGATGGA AGCTGAAGAA
    GAAGAAGCTT TAGCAATTGC CATGGCAATG TCAGCGTCTC AGGATGCCCT CCCCATAGTT
    CCTCAACTAC AGGTTGAAAA TGGAGAAGAT ATCATCATTA TTCAACAGGA TACACCGGAA
    ACTCTACCAG GACATACCAA AGCAAAACAA CCTTACAGAG AAGACACTGA ATGGCTGAAA
    GGTCAGCAGA TAGGCCTTGG AGCATTTTCT TCTTGTTACC AGGCTCAGGA TGTGGGAACT
    GGAACTTTAA TGGCTGTTAA ACAGGTGACA TATGTCAGAA ATACATCTTC TGAACAAGAA
    GAAGTAGTGG AAGCATTAAG AGAAGAGATC AGAATGATGA GCCATTTGAA TCATCCCAAC
    ATCATTAGGA TGTTGGGAGC CACATGTGAG AAGAGCAATT ATAATCTCTT CATTGAATGG
    ATGGCAGGGG GATCTGTGGC TCATTTGCTC AGTAAATACG GGGCCTTCAA GGAATCAGTA
    GTTATTAACT ATACTGAACA GTTACTTCGT GGCCTTTCCT ATCTCCATGA AAACCAGATC
    ATTCATAGAG ATGTCAAAGG TGCCAATTTG CTAATTGACA GCACAGGTCA GAGACTGAGA
    ATTGCAGATT TTGGAGCTGC AGCCAGGTTG GCATCAAAAG GAACTGGTGC AGGAGAGTTT
    CAGGGACAGT TATTGGGAAC AATTGCATTT ATGGCACCTG AGGTACTGAG AGGTCAGCAG
    TATGGTAGGA GCTGTGATGT ATGGAGTGTT GGCTGTGCTA TTATAGAAAT GGCTTGTGCT
    AAACCACCTT GGAATGCAGA AAAACACTCC AATCATCTTG CTTTGATATT TAAGATTGCA
    AGTGCGACGA CTGCTCCATC AATCCCCTCA CATTTGTCTC CTGGTTTACG AGATGTGGCT
    CTTCGGTGTT TAGAACTTCA ACCTCAGGAC AGACCCCCGT CAAGAGAGCT ACTGAAGCAT
    CCGGTCTTCC GTACTACATG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03115
    Clone ID Related Accession (Same CDS sequence) XM_535240.5 , XM_535240.6
    Accession Version XM_535240.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product mitogen-activated protein kinase kinase kinase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_535240.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGGGGCGGCG 
    AGCCCCGAGG CGGGCGGCGG CGGAGCGGCT CTCAAGGCGA GCAGCGCGCC CGCGGCAGCC
    GCGGGGCTGC TGCGGGAGGG GGGCAGCGGG GGCCGCGAGC GGGCTGACTG GCGGCGGCGG
    CAGCTGCGCA AAGTGCGGAG CGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC
    GCCGCCTCGC CGCCGTCCTC CTCCCCTTCC CCGTCGCCGG AGCCCTCGGA CGCCGCGGCG
    GGCGGGAGCG CCTTCCAGCC TCTGGCGGCG CCGCAGCCGC ACGGAGCCGC GAGCCGTGGC
    GGCGCCCGCC CTCCGGAGCC CGCGGCCGCC CCGGACAGCG GCGCCCCGAG CCCCGCGGGG
    GCCGAGCCCG GGGAGAAGCG GACGCCCGCC GCCGAGCCGC CTCCTGCAGC GGCCCCCGCC
    GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAGGGGTTGC ACAAGATGGA CGATCGCCCA
    GAAGAACGAA TGATTAGGGA GAAACTGAAG GCAACCTGTA TGCCTGCCTG GAAGCACGAG
    TGGCTGGAAA GGAGAAACAG GAGAGGCCCT GTGGTGGTAA AACCAATCCC TACTAAAGGA
    GATGGTTCTG AAATTCACAA TCTGGCTACT GAGTCTCAAG GAGAAGGCCA GGCAAGCACT
    GCATCACCCA CTGCTAAAGG CCGACGCAGT CCTTCTCCTG GCAGCTCCCC ATCTGGTCGC
    ACAGTGAAGT CCGAATCTCC GGGAGTTCGG AGAAAAAGAG TTTCCCCGGT GCCTTTTCAG
    AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC
    CCAGAGGAGA CAAACCGCCG TGTAAACAAA GTTATGAGAG CCAGGCTGTA CTTATTGCAA
    CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGACAGCC CGGACAATAA ATACCGGGTG
    TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTGTTT
    GTTATGCTCC GGGTATTTCA ACTGGAACCT TCAGATCCAA TGTTATGGAG AAAAACTTTA
    AAGAATTTTG AGGTTGAAAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC
    AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA
    TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAGGAGGAG
    CAGATGTGTC CTATTTGCTT GTTGGGCATG CTAGATGAAG AGAGCCTCAC GGTGTGTGAA
    GATGGCTGCA GAAACAAGCT GCATCACCAC TGCATGTCAA TCTGGGCAGA AGAATGTAGA
    AGAAATAGAG AGCCTTTAAT ATGTCCCCTT TGTAGGTCTA AGTGGAGATC TCACGATTTC
    TACAGCCATG AATTGTCAAG CCCTGTGGAT TCCCCTTCTT CCCTGCGAGC TGCACAGCAG
    CAAACCATAC AACAGCAGCC TTTGGCTGGA TCACAACGAA GGAGTCAGGA GAGCAATTTT
    AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA
    TGGATTCAGG TATTTGGAAT GGAACTTGTT GGCTGCTTAT TTTCTAAAAA CTGGAATGTA
    AGAGAGATGG CTCTCAGGCG TCTTTCACAC GATGTTAGTG GGGCTTTGCT CTTGGCAAAT
    GGGGAGAGCA CTGGAAACTC TGGGAGCAAC GGTAGAAGCA GTCCGAGTGC TGGAGCCAGC
    AGTGGGTCTT CCCAGACCAG TATCTCAGGA GACGTGGTAG AGGCATGCTG CAACGTTTTG
    TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACATTGAGA
    GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT
    CTCCGGCCAG TGGTAGACAC CATCCTAGTC AAGTGTGCAG ATGCCAATAG TCGTACAAGT
    CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGCC AAGCAGGAGA GTTGGCAGTT
    GGCAGAGAAA TACTTAAAGC TGGATCCATT GGTATTGGTG GCGTGGATTA CGTCTTAAGT
    TGTATTCTTG GAAACCAAAC TGAGTCAAAC AACTGGCAAG AACTTCTGGG CCGCCTTTGT
    CTTGTAGACA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACT
    GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAAA AGCTGCTGTC CCTCTTAACC
    TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCAATGGTTG GTAAACTCTC CCGAAGGATA
    TATTTGAGTT CTGCAAGAAT GGTTACTGCA GTACCCCACG TATTTTCAAA ACTATTAGAA
    ATGCTGAATG TTTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT
    GCAGATGAGG TGGAAATCGC TGAGGCCATC CATCTGGGCA TAGGAGACAC TTTGGATGAT
    CGACATGACA GCTTTTTGCA GGCATCCGTC CCCACCAGCT ATCCAGAAGC CACAGAGAAC
    AGCCCCCTTG AGCGTACAGA CCATTTAGAG AAAACTGGAA AAGGACTGTG TGCTACAAAA
    CTGAGTGCCA ATTCAGAGGA CATTACTGAC AGACTGGGCA GCATTTCCGT AGGACTTCCC
    AGTTCAACAA TGGAGCAACC AAAGCCAATG GTTCAAACAA AAGGCAGACC GCACAGTCAG
    TGTTTGAACT CCTCTCCTTT ATCTCATCAT TCCCCGTTAA TGTTTCCACC CTTGTCAACT
    CCTCCTTCAT CTGCCCCATC TGCACCACCT GGCACTGGAA CAGATGTCTC TAAGCATAGA
    CCTCAGGGAT TCACTCCCTG CAAAATACCT TCTGCATCTC CTCAAACACA GCGCAAGTTT
    TCTCTACAGT TCCAGAGAAA CTGTTCCGAA AACAGAGACT CAGATAAACT TTCCCCCATT
    TTTACTCAAT CAAGACCCCT GCCCTCTAGT AACATACACA GGCCAAAGCC ATCTCGACCT
    ACCCCAGGTA ATACAAGTAA ACAGGGAGAT GCCTCAAAGA ATAGCATGAC ACTTGATCTG
    AACAGTACTT CCAAATGTGA TGACAGCTTT GGCAGCAGCA GCAATAGTAG TAATGCTCTT
    ATACCCAGTG ATGAGACAGT GTTCACCCCA GTAGAAGAGA AATGCAGGTT AGATGTCAAC
    ACAGAACTCA ACTCCAGTAT TGAGGACCTT CTTGAAGCAT CCATGCCTTC AAGTGACACG
    ACAGTAACTT TTAAGTCAGA AGTTGCTGTC CTCTCTCCTG AAAAGGATGA AAATGATGAT
    ACCTACAAAG ATGATGTGAA TCATAATCAG AAATGCAAAG AAAAGATGGA AGCTGAAGAA
    GAAGAAGCTT TAGCAATTGC CATGGCAATG TCAGCGTCTC AGGATGCCCT CCCCATAGTT
    CCTCAACTAC AGGTTGAAAA TGGAGAAGAT ATCATCATTA TTCAACAGGA TACACCGGAA
    ACTCTACCAG GACATACCAA AGCAAAACAA CCTTACAGAG AAGACACTGA ATGGCTGAAA
    GGTCAGCAGA TAGGCCTTGG AGCATTTTCT TCTTGTTACC AGGCTCAGGA TGTGGGAACT
    GGAACTTTAA TGGCTGTTAA ACAGGTGACA TATGTCAGAA ATACATCTTC TGAACAAGAA
    GAAGTAGTGG AAGCATTAAG AGAAGAGATC AGAATGATGA GCCATTTGAA TCATCCCAAC
    ATCATTAGGA TGTTGGGAGC CACATGTGAG AAGAGCAATT ATAATCTCTT CATTGAATGG
    ATGGCAGGGG GATCTGTGGC TCATTTGCTC AGTAAATACG GGGCCTTCAA GGAATCAGTA
    GTTATTAACT ATACTGAACA GTTACTTCGT GGCCTTTCCT ATCTCCATGA AAACCAGATC
    ATTCATAGAG ATGTCAAAGG TGCCAATTTG CTAATTGACA GCACAGGTCA GAGACTGAGA
    ATTGCAGATT TTGGAGCTGC AGCCAGGTTG GCATCAAAAG GAACTGGTGC AGGAGAGTTT
    CAGGGACAGT TATTGGGAAC AATTGCATTT ATGGCACCTG AGGTACTGAG AGGTCAGCAG
    TATGGTAGGA GCTGTGATGT ATGGAGTGTT GGCTGTGCTA TTATAGAAAT GGCTTGTGCT
    AAACCACCTT GGAATGCAGA AAAACACTCC AATCATCTTG CTTTGATATT TAAGATTGCA
    AGTGCGACGA CTGCTCCATC AATCCCCTCA CATTTGTCTC CTGGTTTACG AGATGTGGCT
    CTTCGGTGTT TAGAACTTCA ACCTCAGGAC AGACCCCCGT CAAGAGAGCT ACTGAAGCAT
    CCGGTCTTCC GTACTACATG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_535240.4
    CDS19..4542
    Translation

    Target ORF information:

    RefSeq Version XM_535240.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_535240.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGGGGCGGCG 
    AGCCCCGAGG CGGGCGGCGG CGGAGCGGCT CTCAAGGCGA GCAGCGCGCC CGCGGCAGCC
    GCGGGGCTGC TGCGGGAGGG GGGCAGCGGG GGCCGCGAGC GGGCTGACTG GCGGCGGCGG
    CAGCTGCGCA AAGTGCGGAG CGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC
    GCCGCCTCGC CGCCGTCCTC CTCCCCTTCC CCGTCGCCGG AGCCCTCGGA CGCCGCGGCG
    GGCGGGAGCG CCTTCCAGCC TCTGGCGGCG CCGCAGCCGC ACGGAGCCGC GAGCCGTGGC
    GGCGCCCGCC CTCCGGAGCC CGCGGCCGCC CCGGACAGCG GCGCCCCGAG CCCCGCGGGG
    GCCGAGCCCG GGGAGAAGCG GACGCCCGCC GCCGAGCCGC CTCCTGCAGC GGCCCCCGCC
    GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAGGGGTTGC ACAAGATGGA CGATCGCCCA
    GAAGAACGAA TGATTAGGGA GAAACTGAAG GCAACCTGTA TGCCTGCCTG GAAGCACGAG
    TGGCTGGAAA GGAGAAACAG GAGAGGCCCT GTGGTGGTAA AACCAATCCC TACTAAAGGA
    GATGGTTCTG AAATTCACAA TCTGGCTACT GAGTCTCAAG GAGAAGGCCA GGCAAGCACT
    GCATCACCCA CTGCTAAAGG CCGACGCAGT CCTTCTCCTG GCAGCTCCCC ATCTGGTCGC
    ACAGTGAAGT CCGAATCTCC GGGAGTTCGG AGAAAAAGAG TTTCCCCGGT GCCTTTTCAG
    AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC
    CCAGAGGAGA CAAACCGCCG TGTAAACAAA GTTATGAGAG CCAGGCTGTA CTTATTGCAA
    CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGACAGCC CGGACAATAA ATACCGGGTG
    TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTGTTT
    GTTATGCTCC GGGTATTTCA ACTGGAACCT TCAGATCCAA TGTTATGGAG AAAAACTTTA
    AAGAATTTTG AGGTTGAAAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC
    AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA
    TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAGGAGGAG
    CAGATGTGTC CTATTTGCTT GTTGGGCATG CTAGATGAAG AGAGCCTCAC GGTGTGTGAA
    GATGGCTGCA GAAACAAGCT GCATCACCAC TGCATGTCAA TCTGGGCAGA AGAATGTAGA
    AGAAATAGAG AGCCTTTAAT ATGTCCCCTT TGTAGGTCTA AGTGGAGATC TCACGATTTC
    TACAGCCATG AATTGTCAAG CCCTGTGGAT TCCCCTTCTT CCCTGCGAGC TGCACAGCAG
    CAAACCATAC AACAGCAGCC TTTGGCTGGA TCACAACGAA GGAGTCAGGA GAGCAATTTT
    AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA
    TGGATTCAGG TATTTGGAAT GGAACTTGTT GGCTGCTTAT TTTCTAAAAA CTGGAATGTA
    AGAGAGATGG CTCTCAGGCG TCTTTCACAC GATGTTAGTG GGGCTTTGCT CTTGGCAAAT
    GGGGAGAGCA CTGGAAACTC TGGGAGCAAC GGTAGAAGCA GTCCGAGTGC TGGAGCCAGC
    AGTGGGTCTT CCCAGACCAG TATCTCAGGA GACGTGGTAG AGGCATGCTG CAACGTTTTG
    TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACATTGAGA
    GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT
    CTCCGGCCAG TGGTAGACAC CATCCTAGTC AAGTGTGCAG ATGCCAATAG TCGTACAAGT
    CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGCC AAGCAGGAGA GTTGGCAGTT
    GGCAGAGAAA TACTTAAAGC TGGATCCATT GGTATTGGTG GCGTGGATTA CGTCTTAAGT
    TGTATTCTTG GAAACCAAAC TGAGTCAAAC AACTGGCAAG AACTTCTGGG CCGCCTTTGT
    CTTGTAGACA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACT
    GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAAA AGCTGCTGTC CCTCTTAACC
    TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCAATGGTTG GTAAACTCTC CCGAAGGATA
    TATTTGAGTT CTGCAAGAAT GGTTACTGCA GTACCCCACG TATTTTCAAA ACTATTAGAA
    ATGCTGAATG TTTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT
    GCAGATGAGG TGGAAATCGC TGAGGCCATC CATCTGGGCA TAGGAGACAC TTTGGATGAT
    CGACATGACA GCTTTTTGCA GGCATCCGTC CCCACCAGCT ATCCAGAAGC CACAGAGAAC
    AGCCCCCTTG AGCGTACAGA CCATTTAGAG AAAACTGGAA AAGGACTGTG TGCTACAAAA
    CTGAGTGCCA ATTCAGAGGA CATTACTGAC AGACTGGGCA GCATTTCCGT AGGACTTCCC
    AGTTCAACAA TGGAGCAACC AAAGCCAATG GTTCAAACAA AAGGCAGACC GCACAGTCAG
    TGTTTGAACT CCTCTCCTTT ATCTCATCAT TCCCCGTTAA TGTTTCCACC CTTGTCAACT
    CCTCCTTCAT CTGCCCCATC TGCACCACCT GGCACTGGAA CAGATGTCTC TAAGCATAGA
    CCTCAGGGAT TCACTCCCTG CAAAATACCT TCTGCATCTC CTCAAACACA GCGCAAGTTT
    TCTCTACAGT TCCAGAGAAA CTGTTCCGAA AACAGAGACT CAGATAAACT TTCCCCCATT
    TTTACTCAAT CAAGACCCCT GCCCTCTAGT AACATACACA GGCCAAAGCC ATCTCGACCT
    ACCCCAGGTA ATACAAGTAA ACAGGGAGAT GCCTCAAAGA ATAGCATGAC ACTTGATCTG
    AACAGTACTT CCAAATGTGA TGACAGCTTT GGCAGCAGCA GCAATAGTAG TAATGCTCTT
    ATACCCAGTG ATGAGACAGT GTTCACCCCA GTAGAAGAGA AATGCAGGTT AGATGTCAAC
    ACAGAACTCA ACTCCAGTAT TGAGGACCTT CTTGAAGCAT CCATGCCTTC AAGTGACACG
    ACAGTAACTT TTAAGTCAGA AGTTGCTGTC CTCTCTCCTG AAAAGGATGA AAATGATGAT
    ACCTACAAAG ATGATGTGAA TCATAATCAG AAATGCAAAG AAAAGATGGA AGCTGAAGAA
    GAAGAAGCTT TAGCAATTGC CATGGCAATG TCAGCGTCTC AGGATGCCCT CCCCATAGTT
    CCTCAACTAC AGGTTGAAAA TGGAGAAGAT ATCATCATTA TTCAACAGGA TACACCGGAA
    ACTCTACCAG GACATACCAA AGCAAAACAA CCTTACAGAG AAGACACTGA ATGGCTGAAA
    GGTCAGCAGA TAGGCCTTGG AGCATTTTCT TCTTGTTACC AGGCTCAGGA TGTGGGAACT
    GGAACTTTAA TGGCTGTTAA ACAGGTGACA TATGTCAGAA ATACATCTTC TGAACAAGAA
    GAAGTAGTGG AAGCATTAAG AGAAGAGATC AGAATGATGA GCCATTTGAA TCATCCCAAC
    ATCATTAGGA TGTTGGGAGC CACATGTGAG AAGAGCAATT ATAATCTCTT CATTGAATGG
    ATGGCAGGGG GATCTGTGGC TCATTTGCTC AGTAAATACG GGGCCTTCAA GGAATCAGTA
    GTTATTAACT ATACTGAACA GTTACTTCGT GGCCTTTCCT ATCTCCATGA AAACCAGATC
    ATTCATAGAG ATGTCAAAGG TGCCAATTTG CTAATTGACA GCACAGGTCA GAGACTGAGA
    ATTGCAGATT TTGGAGCTGC AGCCAGGTTG GCATCAAAAG GAACTGGTGC AGGAGAGTTT
    CAGGGACAGT TATTGGGAAC AATTGCATTT ATGGCACCTG AGGTACTGAG AGGTCAGCAG
    TATGGTAGGA GCTGTGATGT ATGGAGTGTT GGCTGTGCTA TTATAGAAAT GGCTTGTGCT
    AAACCACCTT GGAATGCAGA AAAACACTCC AATCATCTTG CTTTGATATT TAAGATTGCA
    AGTGCGACGA CTGCTCCATC AATCCCCTCA CATTTGTCTC CTGGTTTACG AGATGTGGCT
    CTTCGGTGTT TAGAACTTCA ACCTCAGGAC AGACCCCCGT CAAGAGAGCT ACTGAAGCAT
    CCGGTCTTCC GTACTACATG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03118
    Clone ID Related Accession (Same CDS sequence) XM_005617403.1
    Accession Version XM_005617403.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGGGGCGGCG 
    AGCCCCGAGG CGGGCGGCGG CGGAGCGGCT CTCAAGGCGA GCAGCGCGCC CGCGGCAGCC
    GCGGGGCTGC TGCGGGAGGG GGGCAGCGGG GGCCGCGAGC GGGCTGACTG GCGGCGGCGG
    CAGCTGCGCA AAGTGCGGAG CGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC
    GCCGCCTCGC CGCCGTCCTC CTCCCCTTCC CCGTCGCCGG AGCCCTCGGA CGCCGCGGCG
    GGCGGGAGCG CCTTCCAGCC TCTGGCGGCG CCGCAGCCGC ACGGAGCCGC GAGCCGTGGC
    GGCGCCCGCC CTCCGGAGCC CGCGGCCGCC CCGGACAGCG GCGCCCCGAG CCCCGCGGGG
    GCCGAGCCCG GGGAGAAGCG GACGCCCGCC GCCGAGCCGC CTCCTGCAGC GGCCCCCGCC
    GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAGGGGTTGC ACAAGATGGA CGATCGCCCA
    GAAGAACGAA TGATTAGGGA GAAACTGAAG GCAACCTGTA TGCCTGCCTG GAAGCACGAG
    TGGCTGGAAA GGAGAAACAG GAGAGGCCCT GTGGTGGTAA AACCAATCCC TACTAAAGGA
    GATGGTTCTG AAATTCACAA TCTGGCTACT GAGTCTCAAG GAGAAGGCCA GGCAAGCACT
    GCATCACCCA CTGCTAAAGG CCGACGCAGT CCTTCTCCTG GCAGCTCCCC ATCTGGTCGC
    ACAGTGAAGT CCGAATCTCC GGGAGTTCGG AGAAAAAGAG TTTCCCCGGT GCCTTTTCAG
    AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC
    CCAGAGGAGA CAAACCGCCG TGTAAACAAA GTTATGAGAG CCAGGCTGTA CTTATTGCAA
    CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGACAGCC CGGACAATAA ATACCGGGTG
    TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTGTTT
    GTTATGCTCC GGGTATTTCA ACTGGAACCT TCAGATCCAA TGTTATGGAG AAAAACTTTA
    AAGAATTTTG AGGTTGAAAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC
    AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA
    TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAGGAGGAG
    CAGATGTGTC CTATTTGCTT GTTGGGCATG CTAGATGAAG AGAGCCTCAC GGTGTGTGAA
    GATGGCTGCA GAAACAAGCT GCATCACCAC TGCATGTCAA TCTGGGCAGA AGAATGTAGA
    AGAAATAGAG AGCCTTTAAT ATGTCCCCTT TGTAGGTCTA AGTGGAGATC TCACGATTTC
    TACAGCCATG AATTGTCAAG CCCTGTGGAT TCCCCTTCTT CCCTGCGAGC TGCACAGCAG
    CAAACCATAC AACAGCAGCC TTTGGCTGGA TCACAACGAA GGAGTCAGGA GAGCAATTTT
    AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA
    TGGATTCAGG TATTTGGAAT GGAACTTGTT GGCTGCTTAT TTTCTAAAAA CTGGAATGTA
    AGAGAGATGG CTCTCAGGCG TCTTTCACAC GATGTTAGTG GGGCTTTGCT CTTGGCAAAT
    GGGGAGAGCA CTGGAAACTC TGGGAGCAAC GGTAGAAGCA GTCCGAGTGC TGGAGCCAGC
    AGTGGGTCTT CCCAGACCAG TATCTCAGGA GACGTGGTAG AGGCATGCTG CAACGTTTTG
    TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACATTGAGA
    GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT
    CTCCGGCCAG TGGTAGACAC CATCCTAGTC AAGTGTGCAG ATGCCAATAG TCGTACAAGT
    CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGCC AAGCAGGAGA GTTGGCAGTT
    GGCAGAGAAA TACTTAAAGC TGGATCCATT GGTATTGGTG GCGTGGATTA CGTCTTAAGT
    TGTATTCTTG GAAACCAAAC TGAGTCAAAC AACTGGCAAG AACTTCTGGG CCGCCTTTGT
    CTTGTAGACA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACT
    GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAAA AGCTGCTGTC CCTCTTAACC
    TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCAATGGTTG GTAAACTCTC CCGAAGGATA
    TATTTGAGTT CTGCAAGAAT GGTTACTGCA GTACCCCACG TATTTTCAAA ACTATTAGAA
    ATGCTGAATG TTTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT
    GCAGATGAGG TGGAAATCGC TGAGGCCATC CATCTGGGCA TAGGAGACAC TTTGGATGAT
    CGACATGACA GCTTTTTGCA GGCATCCGTC CCCACCAGCT ATCCAGAAGC CACAGAGAAC
    AGCCCCCTTG AGCGTACAGA CCATTTAGAG AAAACTGGAA AAGGACTGTG TGCTACAAAA
    CTGAGTGCCA ATTCAGAGGA CATTACTGAC AGACTGGGCA GCATTTCCGT AGGACTTCCC
    AGTTCAACAA TGGAGCAACC AAAGCCAATG GTTCAAACAA AAGGCAGACC GCACAGTCAG
    TGTTTGAACT CCTCTCCTTT ATCTCATCAT TCCCCGTTAA TGTTTCCACC CTTGTCAACT
    CCTCCTTCAT CTGCCCCATC TGCACCACCT GGCACTGGAA CAGATGTCTC TAAGCATAGA
    CCTCAGGGAT TCACTCCCTG CAAAATACCT TCTGCATCTC CTCAAACACA GCGCAAGTTT
    TCTCTACAGT TCCAGAGAAA CTGTTCCGAA AACAGAGACT CAGATAAACT TTCCCCCATT
    TTTACTCAAT CAAGACCCCT GCCCTCTAGT AACATACACA GGCCAAAGCC ATCTCGACCT
    ACCCCAGGTA ATACAAGTAA ACAGGGAGAT GCCTCAAAGA ATAGCATGAC ACTTGATCTG
    AACAGTACTT CCAAATGTGA TGACAGCTTT GGCAGCAGCA GCAATAGTAG TAATGCTCTT
    ATACCCAGTG ATGAGACAGT GTTCACCCCA GTAGAAGAGA AATGCAGGTT AGATGTCAAC
    ACAGAACTCA ACTCCAGTAT TGAGGACCTT CTTGAAGCAT CCATGCCTTC AAGTGACACG
    ACAGTAACTT TTAAGTCAGA AGTTGCTGTC CTCTCTCCTG AAAAGGATGA AAATGATGAT
    ACCTACAAAG ATGATGTGAA TCATAATCAG AAATGCAAAG AAAAGATGGA AGCTGAAGAA
    GAAGAAGCTT TAGCAATTGC CATGGCAATG TCAGCGTCTC AGGATGCCCT CCCCATAGTT
    CCTCAACTAC AGGTTGAAAA TGGAGAAGAT ATCATCATTA TTCAACAGGA TACACCGGAA
    ACTCTACCAG GACATACCAA AGCAAAACAA CCTTACAGAG AAGACACTGA ATGGCTGAAA
    GGTCAGCAGA TAGGCCTTGG AGCATTTTCT TCTTGTTACC AGGCTCAGGA TGTGGGAACT
    GGAACTTTAA TGGCTGTTAA ACAGATCAGA TTGTTAGTAA AAATTGTAAA TGGAAATTCA
    TCAGAATTCA AAACTTTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617460.1
    CDS13..3873
    Translation

    Target ORF information:

    RefSeq Version XM_005617403.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617403.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGGGGCGGCG 
    AGCCCCGAGG CGGGCGGCGG CGGAGCGGCT CTCAAGGCGA GCAGCGCGCC CGCGGCAGCC
    GCGGGGCTGC TGCGGGAGGG GGGCAGCGGG GGCCGCGAGC GGGCTGACTG GCGGCGGCGG
    CAGCTGCGCA AAGTGCGGAG CGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC
    GCCGCCTCGC CGCCGTCCTC CTCCCCTTCC CCGTCGCCGG AGCCCTCGGA CGCCGCGGCG
    GGCGGGAGCG CCTTCCAGCC TCTGGCGGCG CCGCAGCCGC ACGGAGCCGC GAGCCGTGGC
    GGCGCCCGCC CTCCGGAGCC CGCGGCCGCC CCGGACAGCG GCGCCCCGAG CCCCGCGGGG
    GCCGAGCCCG GGGAGAAGCG GACGCCCGCC GCCGAGCCGC CTCCTGCAGC GGCCCCCGCC
    GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAGGGGTTGC ACAAGATGGA CGATCGCCCA
    GAAGAACGAA TGATTAGGGA GAAACTGAAG GCAACCTGTA TGCCTGCCTG GAAGCACGAG
    TGGCTGGAAA GGAGAAACAG GAGAGGCCCT GTGGTGGTAA AACCAATCCC TACTAAAGGA
    GATGGTTCTG AAATTCACAA TCTGGCTACT GAGTCTCAAG GAGAAGGCCA GGCAAGCACT
    GCATCACCCA CTGCTAAAGG CCGACGCAGT CCTTCTCCTG GCAGCTCCCC ATCTGGTCGC
    ACAGTGAAGT CCGAATCTCC GGGAGTTCGG AGAAAAAGAG TTTCCCCGGT GCCTTTTCAG
    AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC
    CCAGAGGAGA CAAACCGCCG TGTAAACAAA GTTATGAGAG CCAGGCTGTA CTTATTGCAA
    CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGACAGCC CGGACAATAA ATACCGGGTG
    TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTGTTT
    GTTATGCTCC GGGTATTTCA ACTGGAACCT TCAGATCCAA TGTTATGGAG AAAAACTTTA
    AAGAATTTTG AGGTTGAAAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC
    AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA
    TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAGGAGGAG
    CAGATGTGTC CTATTTGCTT GTTGGGCATG CTAGATGAAG AGAGCCTCAC GGTGTGTGAA
    GATGGCTGCA GAAACAAGCT GCATCACCAC TGCATGTCAA TCTGGGCAGA AGAATGTAGA
    AGAAATAGAG AGCCTTTAAT ATGTCCCCTT TGTAGGTCTA AGTGGAGATC TCACGATTTC
    TACAGCCATG AATTGTCAAG CCCTGTGGAT TCCCCTTCTT CCCTGCGAGC TGCACAGCAG
    CAAACCATAC AACAGCAGCC TTTGGCTGGA TCACAACGAA GGAGTCAGGA GAGCAATTTT
    AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA
    TGGATTCAGG TATTTGGAAT GGAACTTGTT GGCTGCTTAT TTTCTAAAAA CTGGAATGTA
    AGAGAGATGG CTCTCAGGCG TCTTTCACAC GATGTTAGTG GGGCTTTGCT CTTGGCAAAT
    GGGGAGAGCA CTGGAAACTC TGGGAGCAAC GGTAGAAGCA GTCCGAGTGC TGGAGCCAGC
    AGTGGGTCTT CCCAGACCAG TATCTCAGGA GACGTGGTAG AGGCATGCTG CAACGTTTTG
    TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACATTGAGA
    GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT
    CTCCGGCCAG TGGTAGACAC CATCCTAGTC AAGTGTGCAG ATGCCAATAG TCGTACAAGT
    CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGCC AAGCAGGAGA GTTGGCAGTT
    GGCAGAGAAA TACTTAAAGC TGGATCCATT GGTATTGGTG GCGTGGATTA CGTCTTAAGT
    TGTATTCTTG GAAACCAAAC TGAGTCAAAC AACTGGCAAG AACTTCTGGG CCGCCTTTGT
    CTTGTAGACA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACT
    GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAAA AGCTGCTGTC CCTCTTAACC
    TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCAATGGTTG GTAAACTCTC CCGAAGGATA
    TATTTGAGTT CTGCAAGAAT GGTTACTGCA GTACCCCACG TATTTTCAAA ACTATTAGAA
    ATGCTGAATG TTTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT
    GCAGATGAGG TGGAAATCGC TGAGGCCATC CATCTGGGCA TAGGAGACAC TTTGGATGAT
    CGACATGACA GCTTTTTGCA GGCATCCGTC CCCACCAGCT ATCCAGAAGC CACAGAGAAC
    AGCCCCCTTG AGCGTACAGA CCATTTAGAG AAAACTGGAA AAGGACTGTG TGCTACAAAA
    CTGAGTGCCA ATTCAGAGGA CATTACTGAC AGACTGGGCA GCATTTCCGT AGGACTTCCC
    AGTTCAACAA TGGAGCAACC AAAGCCAATG GTTCAAACAA AAGGCAGACC GCACAGTCAG
    TGTTTGAACT CCTCTCCTTT ATCTCATCAT TCCCCGTTAA TGTTTCCACC CTTGTCAACT
    CCTCCTTCAT CTGCCCCATC TGCACCACCT GGCACTGGAA CAGATGTCTC TAAGCATAGA
    CCTCAGGGAT TCACTCCCTG CAAAATACCT TCTGCATCTC CTCAAACACA GCGCAAGTTT
    TCTCTACAGT TCCAGAGAAA CTGTTCCGAA AACAGAGACT CAGATAAACT TTCCCCCATT
    TTTACTCAAT CAAGACCCCT GCCCTCTAGT AACATACACA GGCCAAAGCC ATCTCGACCT
    ACCCCAGGTA ATACAAGTAA ACAGGGAGAT GCCTCAAAGA ATAGCATGAC ACTTGATCTG
    AACAGTACTT CCAAATGTGA TGACAGCTTT GGCAGCAGCA GCAATAGTAG TAATGCTCTT
    ATACCCAGTG ATGAGACAGT GTTCACCCCA GTAGAAGAGA AATGCAGGTT AGATGTCAAC
    ACAGAACTCA ACTCCAGTAT TGAGGACCTT CTTGAAGCAT CCATGCCTTC AAGTGACACG
    ACAGTAACTT TTAAGTCAGA AGTTGCTGTC CTCTCTCCTG AAAAGGATGA AAATGATGAT
    ACCTACAAAG ATGATGTGAA TCATAATCAG AAATGCAAAG AAAAGATGGA AGCTGAAGAA
    GAAGAAGCTT TAGCAATTGC CATGGCAATG TCAGCGTCTC AGGATGCCCT CCCCATAGTT
    CCTCAACTAC AGGTTGAAAA TGGAGAAGAT ATCATCATTA TTCAACAGGA TACACCGGAA
    ACTCTACCAG GACATACCAA AGCAAAACAA CCTTACAGAG AAGACACTGA ATGGCTGAAA
    GGTCAGCAGA TAGGCCTTGG AGCATTTTCT TCTTGTTACC AGGCTCAGGA TGTGGGAACT
    GGAACTTTAA TGGCTGTTAA ACAGATCAGA TTGTTAGTAA AAATTGTAAA TGGAAATTCA
    TCAGAATTCA AAACTTTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.