MAP3K1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K1
Entrez Gene ID	478061
Full Name	mitogen-activated protein kinase kinase kinase 1
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 1 Names mitogen-activated protein kinase kinase kinase 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005617401.2	XP_005617458.1	mitogen-activated protein kinase kinase kinase 1 isoform X2
XM_535240.5	XP_535240.4	mitogen-activated protein kinase kinase kinase 1 isoform X1
XM_005617402.2	XP_005617459.1	mitogen-activated protein kinase kinase kinase 1 isoform X3
XM_005617403.1	XP_005617460.1	mitogen-activated protein kinase kinase kinase 1 isoform X4
XM_535240.6	XP_535240.4	mitogen-activated protein kinase kinase kinase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAP3K1	NP_005912.1
Rattus norvegicus (Norway rat)	LOC100912399	XP_006231988.1
	MAP3K1	XP_002804414.1
Canis lupus familiaris (dog)	MAP3K1	XP_535240.4
Xenopus tropicalis (tropical clawed frog)	map3k1	XP_002940279.2
Rattus norvegicus (Norway rat)	Map3k1	NP_446339.1
Pan troglodytes (chimpanzee)	MAP3K1	XP_003310777.1
Bos taurus (cattle)	MAP3K1	NP_001192835.1
Mus musculus (house mouse)	Map3k1	NP_036075.2
Gallus gallus (chicken)	MAP3K1	XP_424734.3
Danio rerio (zebrafish)	map3k1	XP_002663531.2

Gene Ontology

The following MAP3K1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe03116	XM_005617401.2 Latest version!	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe03117	XM_005617402.2 Latest version!	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe03115	XM_535240.6 Latest version!	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe03118	XM_005617403.1 Latest version!	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
OCe03115	XM_535240.5	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K1 ( XM_005617401.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617458.1 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe03116
Clone ID Related Accession (Same CDS sequence)	XM_005617401.2
Accession Version	XM_005617401.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617401.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGTG AGATGGAGAA TAAAGAAACT CTCAAGGGGT TGCACAAGAT GGACGATCGC 
CCAGAAGAAC GAATGATTAG GGAGAAACTG AAGGCAACCT GTATGCCTGC CTGGAAGCAC 
GAGTGGCTGG AAAGGAGAAA CAGGAGAGGC CCTGTGGTGG TAAAACCAAT CCCTACTAAA 
GGAGATGGTT CTGAAATTCA CAATCTGGCT ACTGAGTCTC AAGGAGAAGG CCAGGCAAGC 
ACTGCATCAC CCACTGCTAA AGGCCGACGC AGTCCTTCTC CTGGCAGCTC CCCATCTGGT 
CGCACAGTGA AGTCCGAATC TCCGGGAGTT CGGAGAAAAA GAGTTTCCCC GGTGCCTTTT 
CAGAGTGGGA GAATCACACC ACCTCGAAGA GCCCCTTCAC CAGATGGCTT CTCACCATAT 
AGCCCAGAGG AGACAAACCG CCGTGTAAAC AAAGTTATGA GAGCCAGGCT GTACTTATTG 
CAACAAATAG GACCCAACTC TTTCCTGATT GGAGGAGACA GCCCGGACAA TAAATACCGG 
GTGTTTATTG GGCCTCAGAA CTGCAGCTGT GGGCGTGGAA CATTTTGTAT TCATCTGTTG 
TTTGTTATGC TCCGGGTATT TCAACTGGAA CCTTCAGATC CAATGTTATG GAGAAAAACT 
TTAAAGAATT TTGAGGTTGA AAGTTTGTTC CAGAAATATC ACAGTAGGCG TAGCTCAAGG 
ATCAAAGCTC CATCTCGTAA CACCATCCAG AAGTTTGTTT CACGCATGTC AAATTCTCAT 
ACATTGTCAT CATCTAGTAC TTCTACATCT AGTTCAGAAA ACAGTATAAA AGATGAGGAG 
GAGCAGATGT GTCCTATTTG CTTGTTGGGC ATGCTAGATG AAGAGAGCCT CACGGTGTGT 
GAAGATGGCT GCAGAAACAA GCTGCATCAC CACTGCATGT CAATCTGGGC AGAAGAATGT 
AGAAGAAATA GAGAGCCTTT AATATGTCCC CTTTGTAGGT CTAAGTGGAG ATCTCACGAT 
TTCTACAGCC ATGAATTGTC AAGCCCTGTG GATTCCCCTT CTTCCCTGCG AGCTGCACAG 
CAGCAAACCA TACAACAGCA GCCTTTGGCT GGATCACAAC GAAGGAGTCA GGAGAGCAAT 
TTTAACCTTA CTCATTATGG AACTCAGCAG ATTCCTCCTG CTTATAAAGA TTTAGCTGAG 
CCATGGATTC AGGTATTTGG AATGGAACTT GTTGGCTGCT TATTTTCTAA AAACTGGAAT 
GTAAGAGAGA TGGCTCTCAG GCGTCTTTCA CACGATGTTA GTGGGGCTTT GCTCTTGGCA 
AATGGGGAGA GCACTGGAAA CTCTGGGAGC AACGGTAGAA GCAGTCCGAG TGCTGGAGCC 
AGCAGTGGGT CTTCCCAGAC CAGTATCTCA GGAGACGTGG TAGAGGCATG CTGCAACGTT 
TTGTCAATGG TCTGTGCTGA CCCTGTCTAC AAAGTGTACG TTGCTGCTTT AAAAACATTG 
AGAGCCATGC TAGTATATAC TCCTTGCCAT AGTTTGGCAG AAAGAATCAA ACTGCAGAGA 
CTTCTCCGGC CAGTGGTAGA CACCATCCTA GTCAAGTGTG CAGATGCCAA TAGTCGTACA 
AGTCAGCTGT CTATATCAAC ACTGTTGGAA TTATGCAAAG GCCAAGCAGG AGAGTTGGCA 
GTTGGCAGAG AAATACTTAA AGCTGGATCC ATTGGTATTG GTGGCGTGGA TTACGTCTTA 
AGTTGTATTC TTGGAAACCA AACTGAGTCA AACAACTGGC AAGAACTTCT GGGCCGCCTT 
TGTCTTGTAG ACAGACTGCT GTTGGAATTT CCTGCTGAAT TTTACCCTCA TATTGTCAGT 
ACTGATGTTT CACAAGCTGA GCCTGTTGAA GTCAGGTATA AAAAGCTGCT GTCCCTCTTA 
ACCTTTGCTT TGCAGTCCAT TGATAATTCC CACTCAATGG TTGGTAAACT CTCCCGAAGG 
ATATATTTGA GTTCTGCAAG AATGGTTACT GCAGTACCCC ACGTATTTTC AAAACTATTA 
GAAATGCTGA ATGTTTCCAG TTCCACTCAT TTTACCAGGA TGCGTCGTCG TTTGATGGCT 
ATTGCAGATG AGGTGGAAAT CGCTGAGGCC ATCCATCTGG GCATAGGAGA CACTTTGGAT 
GATCGACATG ACAGCTTTTT GCAGGCATCC GTCCCCACCA GCTATCCAGA AGCCACAGAG 
AACAGCCCCC TTGAGCGTAC AGACCATTTA GAGAAAACTG GAAAAGGACT GTGTGCTACA 
AAACTGAGTG CCAATTCAGA GGACATTACT GACAGACTGG GCAGCATTTC CGTAGGACTT 
CCCAGTTCAA CAATGGAGCA ACCAAAGCCA ATGGTTCAAA CAAAAGGCAG ACCGCACAGT 
CAGTGTTTGA ACTCCTCTCC TTTATCTCAT CATTCCCCGT TAATGTTTCC ACCCTTGTCA 
ACTCCTCCTT CATCTGCCCC ATCTGCACCA CCTGGCACTG GAACAGATGT CTCTAAGCAT 
AGACCTCAGG GATTCACTCC CTGCAAAATA CCTTCTGCAT CTCCTCAAAC ACAGCGCAAG 
TTTTCTCTAC AGTTCCAGAG AAACTGTTCC GAAAACAGAG ACTCAGATAA ACTTTCCCCC 
ATTTTTACTC AATCAAGACC CCTGCCCTCT AGTAACATAC ACAGGCCAAA GCCATCTCGA 
CCTACCCCAG GTAATACAAG TAAACAGGGA GATGCCTCAA AGAATAGCAT GACACTTGAT 
CTGAACAGTA CTTCCAAATG TGATGACAGC TTTGGCAGCA GCAGCAATAG TAGTAATGCT 
CTTATACCCA GTGATGAGAC AGTGTTCACC CCAGTAGAAG AGAAATGCAG GTTAGATGTC 
AACACAGAAC TCAACTCCAG TATTGAGGAC CTTCTTGAAG CATCCATGCC TTCAAGTGAC 
ACGACAGTAA CTTTTAAGTC AGAAGTTGCT GTCCTCTCTC CTGAAAAGGA TGAAAATGAT 
GATACCTACA AAGATGATGT GAATCATAAT CAGAAATGCA AAGAAAAGAT GGAAGCTGAA 
GAAGAAGAAG CTTTAGCAAT TGCCATGGCA ATGTCAGCGT CTCAGGATGC CCTCCCCATA 
GTTCCTCAAC TACAGGTTGA AAATGGAGAA GATATCATCA TTATTCAACA GGATACACCG 
GAAACTCTAC CAGGACATAC CAAAGCAAAA CAACCTTACA GAGAAGACAC TGAATGGCTG 
AAAGGTCAGC AGATAGGCCT TGGAGCATTT TCTTCTTGTT ACCAGGCTCA GGATGTGGGA 
ACTGGAACTT TAATGGCTGT TAAACAGGTG ACATATGTCA GAAATACATC TTCTGAACAA 
GAAGAAGTAG TGGAAGCATT AAGAGAAGAG ATCAGAATGA TGAGCCATTT GAATCATCCC 
AACATCATTA GGATGTTGGG AGCCACATGT GAGAAGAGCA ATTATAATCT CTTCATTGAA 
TGGATGGCAG GGGGATCTGT GGCTCATTTG CTCAGTAAAT ACGGGGCCTT CAAGGAATCA 
GTAGTTATTA ACTATACTGA ACAGTTACTT CGTGGCCTTT CCTATCTCCA TGAAAACCAG 
ATCATTCATA GAGATGTCAA AGGTGCCAAT TTGCTAATTG ACAGCACAGG TCAGAGACTG 
AGAATTGCAG ATTTTGGAGC TGCAGCCAGG TTGGCATCAA AAGGAACTGG TGCAGGAGAG 
TTTCAGGGAC AGTTATTGGG AACAATTGCA TTTATGGCAC CTGAGGTACT GAGAGGTCAG 
CAGTATGGTA GGAGCTGTGA TGTATGGAGT GTTGGCTGTG CTATTATAGA AATGGCTTGT 
GCTAAACCAC CTTGGAATGC AGAAAAACAC TCCAATCATC TTGCTTTGAT ATTTAAGATT 
GCAAGTGCGA CGACTGCTCC ATCAATCCCC TCACATTTGT CTCCTGGTTT ACGAGATGTG 
GCTCTTCGGT GTTTAGAACT TCAACCTCAG GACAGACCCC CGTCAAGAGA GCTACTGAAG 
CATCCGGTCT TCCGTACTAC ATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617458.1
CDS	88..4134
Translation	MSREMENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPTKGDGSEIHNLATESQGEGQASTASPTAKGRRSPSPGSSPSGRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTIQQQPLAGSQRRSQESNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSKNWNVREMALRRLSHDVSGALLLANGESTGNSGSNGRSSPSAGASSGSSQTSISGDVVEACCNVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLSCILGNQTESNNWQELLGRLCLVDRLLLEFPAEFYPHIVSTDVSQAEPVEVRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPHVFSKLLEMLNVSSSTHFTRMRRRLMAIADEVEIAEAIHLGIGDTLDDRHDSFLQASVPTSYPEATENSPLERTDHLEKTGKGLCATKLSANSEDITDRLGSISVGLPSSTMEQPKPMVQTKGRPHSQCLNSSPLSHHSPLMFPPLSTPPSSAPSAPPGTGTDVSKHRPQGFTPCKIPSASPQTQRKFSLQFQRNCSENRDSDKLSPIFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDASKNSMTLDLNSTSKCDDSFGSSSNSSNALIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKDENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_005617401.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617401.2

ORF Insert Sequence:

ATGAGTCGTG AGATGGAGAA TAAAGAAACT CTCAAGGGGT TGCACAAGAT GGACGATCGC 
CCAGAAGAAC GAATGATTAG GGAGAAACTG AAGGCAACCT GTATGCCTGC CTGGAAGCAC 
GAGTGGCTGG AAAGGAGAAA CAGGAGAGGC CCTGTGGTGG TAAAACCAAT CCCTACTAAA 
GGAGATGGTT CTGAAATTCA CAATCTGGCT ACTGAGTCTC AAGGAGAAGG CCAGGCAAGC 
ACTGCATCAC CCACTGCTAA AGGCCGACGC AGTCCTTCTC CTGGCAGCTC CCCATCTGGT 
CGCACAGTGA AGTCCGAATC TCCGGGAGTT CGGAGAAAAA GAGTTTCCCC GGTGCCTTTT 
CAGAGTGGGA GAATCACACC ACCTCGAAGA GCCCCTTCAC CAGATGGCTT CTCACCATAT 
AGCCCAGAGG AGACAAACCG CCGTGTAAAC AAAGTTATGA GAGCCAGGCT GTACTTATTG 
CAACAAATAG GACCCAACTC TTTCCTGATT GGAGGAGACA GCCCGGACAA TAAATACCGG 
GTGTTTATTG GGCCTCAGAA CTGCAGCTGT GGGCGTGGAA CATTTTGTAT TCATCTGTTG 
TTTGTTATGC TCCGGGTATT TCAACTGGAA CCTTCAGATC CAATGTTATG GAGAAAAACT 
TTAAAGAATT TTGAGGTTGA AAGTTTGTTC CAGAAATATC ACAGTAGGCG TAGCTCAAGG 
ATCAAAGCTC CATCTCGTAA CACCATCCAG AAGTTTGTTT CACGCATGTC AAATTCTCAT 
ACATTGTCAT CATCTAGTAC TTCTACATCT AGTTCAGAAA ACAGTATAAA AGATGAGGAG 
GAGCAGATGT GTCCTATTTG CTTGTTGGGC ATGCTAGATG AAGAGAGCCT CACGGTGTGT 
GAAGATGGCT GCAGAAACAA GCTGCATCAC CACTGCATGT CAATCTGGGC AGAAGAATGT 
AGAAGAAATA GAGAGCCTTT AATATGTCCC CTTTGTAGGT CTAAGTGGAG ATCTCACGAT 
TTCTACAGCC ATGAATTGTC AAGCCCTGTG GATTCCCCTT CTTCCCTGCG AGCTGCACAG 
CAGCAAACCA TACAACAGCA GCCTTTGGCT GGATCACAAC GAAGGAGTCA GGAGAGCAAT 
TTTAACCTTA CTCATTATGG AACTCAGCAG ATTCCTCCTG CTTATAAAGA TTTAGCTGAG 
CCATGGATTC AGGTATTTGG AATGGAACTT GTTGGCTGCT TATTTTCTAA AAACTGGAAT 
GTAAGAGAGA TGGCTCTCAG GCGTCTTTCA CACGATGTTA GTGGGGCTTT GCTCTTGGCA 
AATGGGGAGA GCACTGGAAA CTCTGGGAGC AACGGTAGAA GCAGTCCGAG TGCTGGAGCC 
AGCAGTGGGT CTTCCCAGAC CAGTATCTCA GGAGACGTGG TAGAGGCATG CTGCAACGTT 
TTGTCAATGG TCTGTGCTGA CCCTGTCTAC AAAGTGTACG TTGCTGCTTT AAAAACATTG 
AGAGCCATGC TAGTATATAC TCCTTGCCAT AGTTTGGCAG AAAGAATCAA ACTGCAGAGA 
CTTCTCCGGC CAGTGGTAGA CACCATCCTA GTCAAGTGTG CAGATGCCAA TAGTCGTACA 
AGTCAGCTGT CTATATCAAC ACTGTTGGAA TTATGCAAAG GCCAAGCAGG AGAGTTGGCA 
GTTGGCAGAG AAATACTTAA AGCTGGATCC ATTGGTATTG GTGGCGTGGA TTACGTCTTA 
AGTTGTATTC TTGGAAACCA AACTGAGTCA AACAACTGGC AAGAACTTCT GGGCCGCCTT 
TGTCTTGTAG ACAGACTGCT GTTGGAATTT CCTGCTGAAT TTTACCCTCA TATTGTCAGT 
ACTGATGTTT CACAAGCTGA GCCTGTTGAA GTCAGGTATA AAAAGCTGCT GTCCCTCTTA 
ACCTTTGCTT TGCAGTCCAT TGATAATTCC CACTCAATGG TTGGTAAACT CTCCCGAAGG 
ATATATTTGA GTTCTGCAAG AATGGTTACT GCAGTACCCC ACGTATTTTC AAAACTATTA 
GAAATGCTGA ATGTTTCCAG TTCCACTCAT TTTACCAGGA TGCGTCGTCG TTTGATGGCT 
ATTGCAGATG AGGTGGAAAT CGCTGAGGCC ATCCATCTGG GCATAGGAGA CACTTTGGAT 
GATCGACATG ACAGCTTTTT GCAGGCATCC GTCCCCACCA GCTATCCAGA AGCCACAGAG 
AACAGCCCCC TTGAGCGTAC AGACCATTTA GAGAAAACTG GAAAAGGACT GTGTGCTACA 
AAACTGAGTG CCAATTCAGA GGACATTACT GACAGACTGG GCAGCATTTC CGTAGGACTT 
CCCAGTTCAA CAATGGAGCA ACCAAAGCCA ATGGTTCAAA CAAAAGGCAG ACCGCACAGT 
CAGTGTTTGA ACTCCTCTCC TTTATCTCAT CATTCCCCGT TAATGTTTCC ACCCTTGTCA 
ACTCCTCCTT CATCTGCCCC ATCTGCACCA CCTGGCACTG GAACAGATGT CTCTAAGCAT 
AGACCTCAGG GATTCACTCC CTGCAAAATA CCTTCTGCAT CTCCTCAAAC ACAGCGCAAG 
TTTTCTCTAC AGTTCCAGAG AAACTGTTCC GAAAACAGAG ACTCAGATAA ACTTTCCCCC 
ATTTTTACTC AATCAAGACC CCTGCCCTCT AGTAACATAC ACAGGCCAAA GCCATCTCGA 
CCTACCCCAG GTAATACAAG TAAACAGGGA GATGCCTCAA AGAATAGCAT GACACTTGAT 
CTGAACAGTA CTTCCAAATG TGATGACAGC TTTGGCAGCA GCAGCAATAG TAGTAATGCT 
CTTATACCCA GTGATGAGAC AGTGTTCACC CCAGTAGAAG AGAAATGCAG GTTAGATGTC 
AACACAGAAC TCAACTCCAG TATTGAGGAC CTTCTTGAAG CATCCATGCC TTCAAGTGAC 
ACGACAGTAA CTTTTAAGTC AGAAGTTGCT GTCCTCTCTC CTGAAAAGGA TGAAAATGAT 
GATACCTACA AAGATGATGT GAATCATAAT CAGAAATGCA AAGAAAAGAT GGAAGCTGAA 
GAAGAAGAAG CTTTAGCAAT TGCCATGGCA ATGTCAGCGT CTCAGGATGC CCTCCCCATA 
GTTCCTCAAC TACAGGTTGA AAATGGAGAA GATATCATCA TTATTCAACA GGATACACCG 
GAAACTCTAC CAGGACATAC CAAAGCAAAA CAACCTTACA GAGAAGACAC TGAATGGCTG 
AAAGGTCAGC AGATAGGCCT TGGAGCATTT TCTTCTTGTT ACCAGGCTCA GGATGTGGGA 
ACTGGAACTT TAATGGCTGT TAAACAGGTG ACATATGTCA GAAATACATC TTCTGAACAA 
GAAGAAGTAG TGGAAGCATT AAGAGAAGAG ATCAGAATGA TGAGCCATTT GAATCATCCC 
AACATCATTA GGATGTTGGG AGCCACATGT GAGAAGAGCA ATTATAATCT CTTCATTGAA 
TGGATGGCAG GGGGATCTGT GGCTCATTTG CTCAGTAAAT ACGGGGCCTT CAAGGAATCA 
GTAGTTATTA ACTATACTGA ACAGTTACTT CGTGGCCTTT CCTATCTCCA TGAAAACCAG 
ATCATTCATA GAGATGTCAA AGGTGCCAAT TTGCTAATTG ACAGCACAGG TCAGAGACTG 
AGAATTGCAG ATTTTGGAGC TGCAGCCAGG TTGGCATCAA AAGGAACTGG TGCAGGAGAG 
TTTCAGGGAC AGTTATTGGG AACAATTGCA TTTATGGCAC CTGAGGTACT GAGAGGTCAG 
CAGTATGGTA GGAGCTGTGA TGTATGGAGT GTTGGCTGTG CTATTATAGA AATGGCTTGT 
GCTAAACCAC CTTGGAATGC AGAAAAACAC TCCAATCATC TTGCTTTGAT ATTTAAGATT 
GCAAGTGCGA CGACTGCTCC ATCAATCCCC TCACATTTGT CTCCTGGTTT ACGAGATGTG 
GCTCTTCGGT GTTTAGAACT TCAACCTCAG GACAGACCCC CGTCAAGAGA GCTACTGAAG 
CATCCGGTCT TCCGTACTAC ATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_005617402.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617459.1 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe03117
Clone ID Related Accession (Same CDS sequence)	XM_005617402.2
Accession Version	XM_005617402.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617402.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA AAGAAACTCT CAAGGGGTTG CACAAGATGG ACGATCGCCC AGAAGAACGA 
ATGATTAGGG AGAAACTGAA GGCAACCTGT ATGCCTGCCT GGAAGCACGA GTGGCTGGAA 
AGGAGAAACA GGAGAGGCCC TGTGGTGGTA AAACCAATCC CTACTAAAGG AGATGGTTCT 
GAAATTCACA ATCTGGCTAC TGAGTCTCAA GGAGAAGGCC AGGCAAGCAC TGCATCACCC 
ACTGCTAAAG GCCGACGCAG TCCTTCTCCT GGCAGCTCCC CATCTGGTCG CACAGTGAAG 
TCCGAATCTC CGGGAGTTCG GAGAAAAAGA GTTTCCCCGG TGCCTTTTCA GAGTGGGAGA 
ATCACACCAC CTCGAAGAGC CCCTTCACCA GATGGCTTCT CACCATATAG CCCAGAGGAG 
ACAAACCGCC GTGTAAACAA AGTTATGAGA GCCAGGCTGT ACTTATTGCA ACAAATAGGA 
CCCAACTCTT TCCTGATTGG AGGAGACAGC CCGGACAATA AATACCGGGT GTTTATTGGG 
CCTCAGAACT GCAGCTGTGG GCGTGGAACA TTTTGTATTC ATCTGTTGTT TGTTATGCTC 
CGGGTATTTC AACTGGAACC TTCAGATCCA ATGTTATGGA GAAAAACTTT AAAGAATTTT 
GAGGTTGAAA GTTTGTTCCA GAAATATCAC AGTAGGCGTA GCTCAAGGAT CAAAGCTCCA 
TCTCGTAACA CCATCCAGAA GTTTGTTTCA CGCATGTCAA ATTCTCATAC ATTGTCATCA 
TCTAGTACTT CTACATCTAG TTCAGAAAAC AGTATAAAAG ATGAGGAGGA GCAGATGTGT 
CCTATTTGCT TGTTGGGCAT GCTAGATGAA GAGAGCCTCA CGGTGTGTGA AGATGGCTGC 
AGAAACAAGC TGCATCACCA CTGCATGTCA ATCTGGGCAG AAGAATGTAG AAGAAATAGA 
GAGCCTTTAA TATGTCCCCT TTGTAGGTCT AAGTGGAGAT CTCACGATTT CTACAGCCAT 
GAATTGTCAA GCCCTGTGGA TTCCCCTTCT TCCCTGCGAG CTGCACAGCA GCAAACCATA 
CAACAGCAGC CTTTGGCTGG ATCACAACGA AGGAGTCAGG AGAGCAATTT TAACCTTACT 
CATTATGGAA CTCAGCAGAT TCCTCCTGCT TATAAAGATT TAGCTGAGCC ATGGATTCAG 
GTATTTGGAA TGGAACTTGT TGGCTGCTTA TTTTCTAAAA ACTGGAATGT AAGAGAGATG 
GCTCTCAGGC GTCTTTCACA CGATGTTAGT GGGGCTTTGC TCTTGGCAAA TGGGGAGAGC 
ACTGGAAACT CTGGGAGCAA CGGTAGAAGC AGTCCGAGTG CTGGAGCCAG CAGTGGGTCT 
TCCCAGACCA GTATCTCAGG AGACGTGGTA GAGGCATGCT GCAACGTTTT GTCAATGGTC 
TGTGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACATTGAG AGCCATGCTA 
GTATATACTC CTTGCCATAG TTTGGCAGAA AGAATCAAAC TGCAGAGACT TCTCCGGCCA 
GTGGTAGACA CCATCCTAGT CAAGTGTGCA GATGCCAATA GTCGTACAAG TCAGCTGTCT 
ATATCAACAC TGTTGGAATT ATGCAAAGGC CAAGCAGGAG AGTTGGCAGT TGGCAGAGAA 
ATACTTAAAG CTGGATCCAT TGGTATTGGT GGCGTGGATT ACGTCTTAAG TTGTATTCTT 
GGAAACCAAA CTGAGTCAAA CAACTGGCAA GAACTTCTGG GCCGCCTTTG TCTTGTAGAC 
AGACTGCTGT TGGAATTTCC TGCTGAATTT TACCCTCATA TTGTCAGTAC TGATGTTTCA 
CAAGCTGAGC CTGTTGAAGT CAGGTATAAA AAGCTGCTGT CCCTCTTAAC CTTTGCTTTG 
CAGTCCATTG ATAATTCCCA CTCAATGGTT GGTAAACTCT CCCGAAGGAT ATATTTGAGT 
TCTGCAAGAA TGGTTACTGC AGTACCCCAC GTATTTTCAA AACTATTAGA AATGCTGAAT 
GTTTCCAGTT CCACTCATTT TACCAGGATG CGTCGTCGTT TGATGGCTAT TGCAGATGAG 
GTGGAAATCG CTGAGGCCAT CCATCTGGGC ATAGGAGACA CTTTGGATGA TCGACATGAC 
AGCTTTTTGC AGGCATCCGT CCCCACCAGC TATCCAGAAG CCACAGAGAA CAGCCCCCTT 
GAGCGTACAG ACCATTTAGA GAAAACTGGA AAAGGACTGT GTGCTACAAA ACTGAGTGCC 
AATTCAGAGG ACATTACTGA CAGACTGGGC AGCATTTCCG TAGGACTTCC CAGTTCAACA 
ATGGAGCAAC CAAAGCCAAT GGTTCAAACA AAAGGCAGAC CGCACAGTCA GTGTTTGAAC 
TCCTCTCCTT TATCTCATCA TTCCCCGTTA ATGTTTCCAC CCTTGTCAAC TCCTCCTTCA 
TCTGCCCCAT CTGCACCACC TGGCACTGGA ACAGATGTCT CTAAGCATAG ACCTCAGGGA 
TTCACTCCCT GCAAAATACC TTCTGCATCT CCTCAAACAC AGCGCAAGTT TTCTCTACAG 
TTCCAGAGAA ACTGTTCCGA AAACAGAGAC TCAGATAAAC TTTCCCCCAT TTTTACTCAA 
TCAAGACCCC TGCCCTCTAG TAACATACAC AGGCCAAAGC CATCTCGACC TACCCCAGGT 
AATACAAGTA AACAGGGAGA TGCCTCAAAG AATAGCATGA CACTTGATCT GAACAGTACT 
TCCAAATGTG ATGACAGCTT TGGCAGCAGC AGCAATAGTA GTAATGCTCT TATACCCAGT 
GATGAGACAG TGTTCACCCC AGTAGAAGAG AAATGCAGGT TAGATGTCAA CACAGAACTC 
AACTCCAGTA TTGAGGACCT TCTTGAAGCA TCCATGCCTT CAAGTGACAC GACAGTAACT 
TTTAAGTCAG AAGTTGCTGT CCTCTCTCCT GAAAAGGATG AAAATGATGA TACCTACAAA 
GATGATGTGA ATCATAATCA GAAATGCAAA GAAAAGATGG AAGCTGAAGA AGAAGAAGCT 
TTAGCAATTG CCATGGCAAT GTCAGCGTCT CAGGATGCCC TCCCCATAGT TCCTCAACTA 
CAGGTTGAAA ATGGAGAAGA TATCATCATT ATTCAACAGG ATACACCGGA AACTCTACCA 
GGACATACCA AAGCAAAACA ACCTTACAGA GAAGACACTG AATGGCTGAA AGGTCAGCAG 
ATAGGCCTTG GAGCATTTTC TTCTTGTTAC CAGGCTCAGG ATGTGGGAAC TGGAACTTTA 
ATGGCTGTTA AACAGGTGAC ATATGTCAGA AATACATCTT CTGAACAAGA AGAAGTAGTG 
GAAGCATTAA GAGAAGAGAT CAGAATGATG AGCCATTTGA ATCATCCCAA CATCATTAGG 
ATGTTGGGAG CCACATGTGA GAAGAGCAAT TATAATCTCT TCATTGAATG GATGGCAGGG 
GGATCTGTGG CTCATTTGCT CAGTAAATAC GGGGCCTTCA AGGAATCAGT AGTTATTAAC 
TATACTGAAC AGTTACTTCG TGGCCTTTCC TATCTCCATG AAAACCAGAT CATTCATAGA 
GATGTCAAAG GTGCCAATTT GCTAATTGAC AGCACAGGTC AGAGACTGAG AATTGCAGAT 
TTTGGAGCTG CAGCCAGGTT GGCATCAAAA GGAACTGGTG CAGGAGAGTT TCAGGGACAG 
TTATTGGGAA CAATTGCATT TATGGCACCT GAGGTACTGA GAGGTCAGCA GTATGGTAGG 
AGCTGTGATG TATGGAGTGT TGGCTGTGCT ATTATAGAAA TGGCTTGTGC TAAACCACCT 
TGGAATGCAG AAAAACACTC CAATCATCTT GCTTTGATAT TTAAGATTGC AAGTGCGACG 
ACTGCTCCAT CAATCCCCTC ACATTTGTCT CCTGGTTTAC GAGATGTGGC TCTTCGGTGT 
TTAGAACTTC AACCTCAGGA CAGACCCCCG TCAAGAGAGC TACTGAAGCA TCCGGTCTTC 
CGTACTACAT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617459.1
CDS	25..4059
Translation	MENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPTKGDGSEIHNLATESQGEGQASTASPTAKGRRSPSPGSSPSGRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTIQQQPLAGSQRRSQESNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSKNWNVREMALRRLSHDVSGALLLANGESTGNSGSNGRSSPSAGASSGSSQTSISGDVVEACCNVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLSCILGNQTESNNWQELLGRLCLVDRLLLEFPAEFYPHIVSTDVSQAEPVEVRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPHVFSKLLEMLNVSSSTHFTRMRRRLMAIADEVEIAEAIHLGIGDTLDDRHDSFLQASVPTSYPEATENSPLERTDHLEKTGKGLCATKLSANSEDITDRLGSISVGLPSSTMEQPKPMVQTKGRPHSQCLNSSPLSHHSPLMFPPLSTPPSSAPSAPPGTGTDVSKHRPQGFTPCKIPSASPQTQRKFSLQFQRNCSENRDSDKLSPIFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDASKNSMTLDLNSTSKCDDSFGSSSNSSNALIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKDENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_005617402.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617402.2

ORF Insert Sequence:

ATGGAGAATA AAGAAACTCT CAAGGGGTTG CACAAGATGG ACGATCGCCC AGAAGAACGA 
ATGATTAGGG AGAAACTGAA GGCAACCTGT ATGCCTGCCT GGAAGCACGA GTGGCTGGAA 
AGGAGAAACA GGAGAGGCCC TGTGGTGGTA AAACCAATCC CTACTAAAGG AGATGGTTCT 
GAAATTCACA ATCTGGCTAC TGAGTCTCAA GGAGAAGGCC AGGCAAGCAC TGCATCACCC 
ACTGCTAAAG GCCGACGCAG TCCTTCTCCT GGCAGCTCCC CATCTGGTCG CACAGTGAAG 
TCCGAATCTC CGGGAGTTCG GAGAAAAAGA GTTTCCCCGG TGCCTTTTCA GAGTGGGAGA 
ATCACACCAC CTCGAAGAGC CCCTTCACCA GATGGCTTCT CACCATATAG CCCAGAGGAG 
ACAAACCGCC GTGTAAACAA AGTTATGAGA GCCAGGCTGT ACTTATTGCA ACAAATAGGA 
CCCAACTCTT TCCTGATTGG AGGAGACAGC CCGGACAATA AATACCGGGT GTTTATTGGG 
CCTCAGAACT GCAGCTGTGG GCGTGGAACA TTTTGTATTC ATCTGTTGTT TGTTATGCTC 
CGGGTATTTC AACTGGAACC TTCAGATCCA ATGTTATGGA GAAAAACTTT AAAGAATTTT 
GAGGTTGAAA GTTTGTTCCA GAAATATCAC AGTAGGCGTA GCTCAAGGAT CAAAGCTCCA 
TCTCGTAACA CCATCCAGAA GTTTGTTTCA CGCATGTCAA ATTCTCATAC ATTGTCATCA 
TCTAGTACTT CTACATCTAG TTCAGAAAAC AGTATAAAAG ATGAGGAGGA GCAGATGTGT 
CCTATTTGCT TGTTGGGCAT GCTAGATGAA GAGAGCCTCA CGGTGTGTGA AGATGGCTGC 
AGAAACAAGC TGCATCACCA CTGCATGTCA ATCTGGGCAG AAGAATGTAG AAGAAATAGA 
GAGCCTTTAA TATGTCCCCT TTGTAGGTCT AAGTGGAGAT CTCACGATTT CTACAGCCAT 
GAATTGTCAA GCCCTGTGGA TTCCCCTTCT TCCCTGCGAG CTGCACAGCA GCAAACCATA 
CAACAGCAGC CTTTGGCTGG ATCACAACGA AGGAGTCAGG AGAGCAATTT TAACCTTACT 
CATTATGGAA CTCAGCAGAT TCCTCCTGCT TATAAAGATT TAGCTGAGCC ATGGATTCAG 
GTATTTGGAA TGGAACTTGT TGGCTGCTTA TTTTCTAAAA ACTGGAATGT AAGAGAGATG 
GCTCTCAGGC GTCTTTCACA CGATGTTAGT GGGGCTTTGC TCTTGGCAAA TGGGGAGAGC 
ACTGGAAACT CTGGGAGCAA CGGTAGAAGC AGTCCGAGTG CTGGAGCCAG CAGTGGGTCT 
TCCCAGACCA GTATCTCAGG AGACGTGGTA GAGGCATGCT GCAACGTTTT GTCAATGGTC 
TGTGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACATTGAG AGCCATGCTA 
GTATATACTC CTTGCCATAG TTTGGCAGAA AGAATCAAAC TGCAGAGACT TCTCCGGCCA 
GTGGTAGACA CCATCCTAGT CAAGTGTGCA GATGCCAATA GTCGTACAAG TCAGCTGTCT 
ATATCAACAC TGTTGGAATT ATGCAAAGGC CAAGCAGGAG AGTTGGCAGT TGGCAGAGAA 
ATACTTAAAG CTGGATCCAT TGGTATTGGT GGCGTGGATT ACGTCTTAAG TTGTATTCTT 
GGAAACCAAA CTGAGTCAAA CAACTGGCAA GAACTTCTGG GCCGCCTTTG TCTTGTAGAC 
AGACTGCTGT TGGAATTTCC TGCTGAATTT TACCCTCATA TTGTCAGTAC TGATGTTTCA 
CAAGCTGAGC CTGTTGAAGT CAGGTATAAA AAGCTGCTGT CCCTCTTAAC CTTTGCTTTG 
CAGTCCATTG ATAATTCCCA CTCAATGGTT GGTAAACTCT CCCGAAGGAT ATATTTGAGT 
TCTGCAAGAA TGGTTACTGC AGTACCCCAC GTATTTTCAA AACTATTAGA AATGCTGAAT 
GTTTCCAGTT CCACTCATTT TACCAGGATG CGTCGTCGTT TGATGGCTAT TGCAGATGAG 
GTGGAAATCG CTGAGGCCAT CCATCTGGGC ATAGGAGACA CTTTGGATGA TCGACATGAC 
AGCTTTTTGC AGGCATCCGT CCCCACCAGC TATCCAGAAG CCACAGAGAA CAGCCCCCTT 
GAGCGTACAG ACCATTTAGA GAAAACTGGA AAAGGACTGT GTGCTACAAA ACTGAGTGCC 
AATTCAGAGG ACATTACTGA CAGACTGGGC AGCATTTCCG TAGGACTTCC CAGTTCAACA 
ATGGAGCAAC CAAAGCCAAT GGTTCAAACA AAAGGCAGAC CGCACAGTCA GTGTTTGAAC 
TCCTCTCCTT TATCTCATCA TTCCCCGTTA ATGTTTCCAC CCTTGTCAAC TCCTCCTTCA 
TCTGCCCCAT CTGCACCACC TGGCACTGGA ACAGATGTCT CTAAGCATAG ACCTCAGGGA 
TTCACTCCCT GCAAAATACC TTCTGCATCT CCTCAAACAC AGCGCAAGTT TTCTCTACAG 
TTCCAGAGAA ACTGTTCCGA AAACAGAGAC TCAGATAAAC TTTCCCCCAT TTTTACTCAA 
TCAAGACCCC TGCCCTCTAG TAACATACAC AGGCCAAAGC CATCTCGACC TACCCCAGGT 
AATACAAGTA AACAGGGAGA TGCCTCAAAG AATAGCATGA CACTTGATCT GAACAGTACT 
TCCAAATGTG ATGACAGCTT TGGCAGCAGC AGCAATAGTA GTAATGCTCT TATACCCAGT 
GATGAGACAG TGTTCACCCC AGTAGAAGAG AAATGCAGGT TAGATGTCAA CACAGAACTC 
AACTCCAGTA TTGAGGACCT TCTTGAAGCA TCCATGCCTT CAAGTGACAC GACAGTAACT 
TTTAAGTCAG AAGTTGCTGT CCTCTCTCCT GAAAAGGATG AAAATGATGA TACCTACAAA 
GATGATGTGA ATCATAATCA GAAATGCAAA GAAAAGATGG AAGCTGAAGA AGAAGAAGCT 
TTAGCAATTG CCATGGCAAT GTCAGCGTCT CAGGATGCCC TCCCCATAGT TCCTCAACTA 
CAGGTTGAAA ATGGAGAAGA TATCATCATT ATTCAACAGG ATACACCGGA AACTCTACCA 
GGACATACCA AAGCAAAACA ACCTTACAGA GAAGACACTG AATGGCTGAA AGGTCAGCAG 
ATAGGCCTTG GAGCATTTTC TTCTTGTTAC CAGGCTCAGG ATGTGGGAAC TGGAACTTTA 
ATGGCTGTTA AACAGGTGAC ATATGTCAGA AATACATCTT CTGAACAAGA AGAAGTAGTG 
GAAGCATTAA GAGAAGAGAT CAGAATGATG AGCCATTTGA ATCATCCCAA CATCATTAGG 
ATGTTGGGAG CCACATGTGA GAAGAGCAAT TATAATCTCT TCATTGAATG GATGGCAGGG 
GGATCTGTGG CTCATTTGCT CAGTAAATAC GGGGCCTTCA AGGAATCAGT AGTTATTAAC 
TATACTGAAC AGTTACTTCG TGGCCTTTCC TATCTCCATG AAAACCAGAT CATTCATAGA 
GATGTCAAAG GTGCCAATTT GCTAATTGAC AGCACAGGTC AGAGACTGAG AATTGCAGAT 
TTTGGAGCTG CAGCCAGGTT GGCATCAAAA GGAACTGGTG CAGGAGAGTT TCAGGGACAG 
TTATTGGGAA CAATTGCATT TATGGCACCT GAGGTACTGA GAGGTCAGCA GTATGGTAGG 
AGCTGTGATG TATGGAGTGT TGGCTGTGCT ATTATAGAAA TGGCTTGTGC TAAACCACCT 
TGGAATGCAG AAAAACACTC CAATCATCTT GCTTTGATAT TTAAGATTGC AAGTGCGACG 
ACTGCTCCAT CAATCCCCTC ACATTTGTCT CCTGGTTTAC GAGATGTGGC TCTTCGGTGT 
TTAGAACTTC AACCTCAGGA CAGACCCCCG TCAAGAGAGC TACTGAAGCA TCCGGTCTTC 
CGTACTACAT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_535240.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_535240.4 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X1, mRNA.

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CloneID	OCe03115
Clone ID Related Accession (Same CDS sequence)	XM_535240.5 , XM_535240.6
Accession Version	XM_535240.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_535240.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGGGGCGGCG 
AGCCCCGAGG CGGGCGGCGG CGGAGCGGCT CTCAAGGCGA GCAGCGCGCC CGCGGCAGCC 
GCGGGGCTGC TGCGGGAGGG GGGCAGCGGG GGCCGCGAGC GGGCTGACTG GCGGCGGCGG 
CAGCTGCGCA AAGTGCGGAG CGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC 
GCCGCCTCGC CGCCGTCCTC CTCCCCTTCC CCGTCGCCGG AGCCCTCGGA CGCCGCGGCG 
GGCGGGAGCG CCTTCCAGCC TCTGGCGGCG CCGCAGCCGC ACGGAGCCGC GAGCCGTGGC 
GGCGCCCGCC CTCCGGAGCC CGCGGCCGCC CCGGACAGCG GCGCCCCGAG CCCCGCGGGG 
GCCGAGCCCG GGGAGAAGCG GACGCCCGCC GCCGAGCCGC CTCCTGCAGC GGCCCCCGCC 
GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAGGGGTTGC ACAAGATGGA CGATCGCCCA 
GAAGAACGAA TGATTAGGGA GAAACTGAAG GCAACCTGTA TGCCTGCCTG GAAGCACGAG 
TGGCTGGAAA GGAGAAACAG GAGAGGCCCT GTGGTGGTAA AACCAATCCC TACTAAAGGA 
GATGGTTCTG AAATTCACAA TCTGGCTACT GAGTCTCAAG GAGAAGGCCA GGCAAGCACT 
GCATCACCCA CTGCTAAAGG CCGACGCAGT CCTTCTCCTG GCAGCTCCCC ATCTGGTCGC 
ACAGTGAAGT CCGAATCTCC GGGAGTTCGG AGAAAAAGAG TTTCCCCGGT GCCTTTTCAG 
AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC 
CCAGAGGAGA CAAACCGCCG TGTAAACAAA GTTATGAGAG CCAGGCTGTA CTTATTGCAA 
CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGACAGCC CGGACAATAA ATACCGGGTG 
TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTGTTT 
GTTATGCTCC GGGTATTTCA ACTGGAACCT TCAGATCCAA TGTTATGGAG AAAAACTTTA 
AAGAATTTTG AGGTTGAAAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 
AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 
TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAGGAGGAG 
CAGATGTGTC CTATTTGCTT GTTGGGCATG CTAGATGAAG AGAGCCTCAC GGTGTGTGAA 
GATGGCTGCA GAAACAAGCT GCATCACCAC TGCATGTCAA TCTGGGCAGA AGAATGTAGA 
AGAAATAGAG AGCCTTTAAT ATGTCCCCTT TGTAGGTCTA AGTGGAGATC TCACGATTTC 
TACAGCCATG AATTGTCAAG CCCTGTGGAT TCCCCTTCTT CCCTGCGAGC TGCACAGCAG 
CAAACCATAC AACAGCAGCC TTTGGCTGGA TCACAACGAA GGAGTCAGGA GAGCAATTTT 
AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA 
TGGATTCAGG TATTTGGAAT GGAACTTGTT GGCTGCTTAT TTTCTAAAAA CTGGAATGTA 
AGAGAGATGG CTCTCAGGCG TCTTTCACAC GATGTTAGTG GGGCTTTGCT CTTGGCAAAT 
GGGGAGAGCA CTGGAAACTC TGGGAGCAAC GGTAGAAGCA GTCCGAGTGC TGGAGCCAGC 
AGTGGGTCTT CCCAGACCAG TATCTCAGGA GACGTGGTAG AGGCATGCTG CAACGTTTTG 
TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACATTGAGA 
GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT 
CTCCGGCCAG TGGTAGACAC CATCCTAGTC AAGTGTGCAG ATGCCAATAG TCGTACAAGT 
CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGCC AAGCAGGAGA GTTGGCAGTT 
GGCAGAGAAA TACTTAAAGC TGGATCCATT GGTATTGGTG GCGTGGATTA CGTCTTAAGT 
TGTATTCTTG GAAACCAAAC TGAGTCAAAC AACTGGCAAG AACTTCTGGG CCGCCTTTGT 
CTTGTAGACA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACT 
GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAAA AGCTGCTGTC CCTCTTAACC 
TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCAATGGTTG GTAAACTCTC CCGAAGGATA 
TATTTGAGTT CTGCAAGAAT GGTTACTGCA GTACCCCACG TATTTTCAAA ACTATTAGAA 
ATGCTGAATG TTTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT 
GCAGATGAGG TGGAAATCGC TGAGGCCATC CATCTGGGCA TAGGAGACAC TTTGGATGAT 
CGACATGACA GCTTTTTGCA GGCATCCGTC CCCACCAGCT ATCCAGAAGC CACAGAGAAC 
AGCCCCCTTG AGCGTACAGA CCATTTAGAG AAAACTGGAA AAGGACTGTG TGCTACAAAA 
CTGAGTGCCA ATTCAGAGGA CATTACTGAC AGACTGGGCA GCATTTCCGT AGGACTTCCC 
AGTTCAACAA TGGAGCAACC AAAGCCAATG GTTCAAACAA AAGGCAGACC GCACAGTCAG 
TGTTTGAACT CCTCTCCTTT ATCTCATCAT TCCCCGTTAA TGTTTCCACC CTTGTCAACT 
CCTCCTTCAT CTGCCCCATC TGCACCACCT GGCACTGGAA CAGATGTCTC TAAGCATAGA 
CCTCAGGGAT TCACTCCCTG CAAAATACCT TCTGCATCTC CTCAAACACA GCGCAAGTTT 
TCTCTACAGT TCCAGAGAAA CTGTTCCGAA AACAGAGACT CAGATAAACT TTCCCCCATT 
TTTACTCAAT CAAGACCCCT GCCCTCTAGT AACATACACA GGCCAAAGCC ATCTCGACCT 
ACCCCAGGTA ATACAAGTAA ACAGGGAGAT GCCTCAAAGA ATAGCATGAC ACTTGATCTG 
AACAGTACTT CCAAATGTGA TGACAGCTTT GGCAGCAGCA GCAATAGTAG TAATGCTCTT 
ATACCCAGTG ATGAGACAGT GTTCACCCCA GTAGAAGAGA AATGCAGGTT AGATGTCAAC 
ACAGAACTCA ACTCCAGTAT TGAGGACCTT CTTGAAGCAT CCATGCCTTC AAGTGACACG 
ACAGTAACTT TTAAGTCAGA AGTTGCTGTC CTCTCTCCTG AAAAGGATGA AAATGATGAT 
ACCTACAAAG ATGATGTGAA TCATAATCAG AAATGCAAAG AAAAGATGGA AGCTGAAGAA 
GAAGAAGCTT TAGCAATTGC CATGGCAATG TCAGCGTCTC AGGATGCCCT CCCCATAGTT 
CCTCAACTAC AGGTTGAAAA TGGAGAAGAT ATCATCATTA TTCAACAGGA TACACCGGAA 
ACTCTACCAG GACATACCAA AGCAAAACAA CCTTACAGAG AAGACACTGA ATGGCTGAAA 
GGTCAGCAGA TAGGCCTTGG AGCATTTTCT TCTTGTTACC AGGCTCAGGA TGTGGGAACT 
GGAACTTTAA TGGCTGTTAA ACAGGTGACA TATGTCAGAA ATACATCTTC TGAACAAGAA 
GAAGTAGTGG AAGCATTAAG AGAAGAGATC AGAATGATGA GCCATTTGAA TCATCCCAAC 
ATCATTAGGA TGTTGGGAGC CACATGTGAG AAGAGCAATT ATAATCTCTT CATTGAATGG 
ATGGCAGGGG GATCTGTGGC TCATTTGCTC AGTAAATACG GGGCCTTCAA GGAATCAGTA 
GTTATTAACT ATACTGAACA GTTACTTCGT GGCCTTTCCT ATCTCCATGA AAACCAGATC 
ATTCATAGAG ATGTCAAAGG TGCCAATTTG CTAATTGACA GCACAGGTCA GAGACTGAGA 
ATTGCAGATT TTGGAGCTGC AGCCAGGTTG GCATCAAAAG GAACTGGTGC AGGAGAGTTT 
CAGGGACAGT TATTGGGAAC AATTGCATTT ATGGCACCTG AGGTACTGAG AGGTCAGCAG 
TATGGTAGGA GCTGTGATGT ATGGAGTGTT GGCTGTGCTA TTATAGAAAT GGCTTGTGCT 
AAACCACCTT GGAATGCAGA AAAACACTCC AATCATCTTG CTTTGATATT TAAGATTGCA 
AGTGCGACGA CTGCTCCATC AATCCCCTCA CATTTGTCTC CTGGTTTACG AGATGTGGCT 
CTTCGGTGTT TAGAACTTCA ACCTCAGGAC AGACCCCCGT CAAGAGAGCT ACTGAAGCAT 
CCGGTCTTCC GTACTACATG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_535240.4
CDS	13..4536
Translation	MAAAAGNRASSSGFPGAGAASPEAGGGGAALKASSAPAAAAGLLREGGSGGRERADWRRRQLRKVRSVELDQLPEPPLFLAASPPSSSPSPSPEPSDAAAGGSAFQPLAAPQPHGAASRGGARPPEPAAAPDSGAPSPAGAEPGEKRTPAAEPPPAAAPAGREMENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPTKGDGSEIHNLATESQGEGQASTASPTAKGRRSPSPGSSPSGRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTIQQQPLAGSQRRSQESNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSKNWNVREMALRRLSHDVSGALLLANGESTGNSGSNGRSSPSAGASSGSSQTSISGDVVEACCNVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLSCILGNQTESNNWQELLGRLCLVDRLLLEFPAEFYPHIVSTDVSQAEPVEVRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPHVFSKLLEMLNVSSSTHFTRMRRRLMAIADEVEIAEAIHLGIGDTLDDRHDSFLQASVPTSYPEATENSPLERTDHLEKTGKGLCATKLSANSEDITDRLGSISVGLPSSTMEQPKPMVQTKGRPHSQCLNSSPLSHHSPLMFPPLSTPPSSAPSAPPGTGTDVSKHRPQGFTPCKIPSASPQTQRKFSLQFQRNCSENRDSDKLSPIFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDASKNSMTLDLNSTSKCDDSFGSSSNSSNALIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKDENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_535240.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_535240.5

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGGGGCGGCG 
AGCCCCGAGG CGGGCGGCGG CGGAGCGGCT CTCAAGGCGA GCAGCGCGCC CGCGGCAGCC 
GCGGGGCTGC TGCGGGAGGG GGGCAGCGGG GGCCGCGAGC GGGCTGACTG GCGGCGGCGG 
CAGCTGCGCA AAGTGCGGAG CGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC 
GCCGCCTCGC CGCCGTCCTC CTCCCCTTCC CCGTCGCCGG AGCCCTCGGA CGCCGCGGCG 
GGCGGGAGCG CCTTCCAGCC TCTGGCGGCG CCGCAGCCGC ACGGAGCCGC GAGCCGTGGC 
GGCGCCCGCC CTCCGGAGCC CGCGGCCGCC CCGGACAGCG GCGCCCCGAG CCCCGCGGGG 
GCCGAGCCCG GGGAGAAGCG GACGCCCGCC GCCGAGCCGC CTCCTGCAGC GGCCCCCGCC 
GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAGGGGTTGC ACAAGATGGA CGATCGCCCA 
GAAGAACGAA TGATTAGGGA GAAACTGAAG GCAACCTGTA TGCCTGCCTG GAAGCACGAG 
TGGCTGGAAA GGAGAAACAG GAGAGGCCCT GTGGTGGTAA AACCAATCCC TACTAAAGGA 
GATGGTTCTG AAATTCACAA TCTGGCTACT GAGTCTCAAG GAGAAGGCCA GGCAAGCACT 
GCATCACCCA CTGCTAAAGG CCGACGCAGT CCTTCTCCTG GCAGCTCCCC ATCTGGTCGC 
ACAGTGAAGT CCGAATCTCC GGGAGTTCGG AGAAAAAGAG TTTCCCCGGT GCCTTTTCAG 
AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC 
CCAGAGGAGA CAAACCGCCG TGTAAACAAA GTTATGAGAG CCAGGCTGTA CTTATTGCAA 
CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGACAGCC CGGACAATAA ATACCGGGTG 
TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTGTTT 
GTTATGCTCC GGGTATTTCA ACTGGAACCT TCAGATCCAA TGTTATGGAG AAAAACTTTA 
AAGAATTTTG AGGTTGAAAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 
AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 
TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAGGAGGAG 
CAGATGTGTC CTATTTGCTT GTTGGGCATG CTAGATGAAG AGAGCCTCAC GGTGTGTGAA 
GATGGCTGCA GAAACAAGCT GCATCACCAC TGCATGTCAA TCTGGGCAGA AGAATGTAGA 
AGAAATAGAG AGCCTTTAAT ATGTCCCCTT TGTAGGTCTA AGTGGAGATC TCACGATTTC 
TACAGCCATG AATTGTCAAG CCCTGTGGAT TCCCCTTCTT CCCTGCGAGC TGCACAGCAG 
CAAACCATAC AACAGCAGCC TTTGGCTGGA TCACAACGAA GGAGTCAGGA GAGCAATTTT 
AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA 
TGGATTCAGG TATTTGGAAT GGAACTTGTT GGCTGCTTAT TTTCTAAAAA CTGGAATGTA 
AGAGAGATGG CTCTCAGGCG TCTTTCACAC GATGTTAGTG GGGCTTTGCT CTTGGCAAAT 
GGGGAGAGCA CTGGAAACTC TGGGAGCAAC GGTAGAAGCA GTCCGAGTGC TGGAGCCAGC 
AGTGGGTCTT CCCAGACCAG TATCTCAGGA GACGTGGTAG AGGCATGCTG CAACGTTTTG 
TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACATTGAGA 
GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT 
CTCCGGCCAG TGGTAGACAC CATCCTAGTC AAGTGTGCAG ATGCCAATAG TCGTACAAGT 
CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGCC AAGCAGGAGA GTTGGCAGTT 
GGCAGAGAAA TACTTAAAGC TGGATCCATT GGTATTGGTG GCGTGGATTA CGTCTTAAGT 
TGTATTCTTG GAAACCAAAC TGAGTCAAAC AACTGGCAAG AACTTCTGGG CCGCCTTTGT 
CTTGTAGACA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACT 
GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAAA AGCTGCTGTC CCTCTTAACC 
TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCAATGGTTG GTAAACTCTC CCGAAGGATA 
TATTTGAGTT CTGCAAGAAT GGTTACTGCA GTACCCCACG TATTTTCAAA ACTATTAGAA 
ATGCTGAATG TTTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT 
GCAGATGAGG TGGAAATCGC TGAGGCCATC CATCTGGGCA TAGGAGACAC TTTGGATGAT 
CGACATGACA GCTTTTTGCA GGCATCCGTC CCCACCAGCT ATCCAGAAGC CACAGAGAAC 
AGCCCCCTTG AGCGTACAGA CCATTTAGAG AAAACTGGAA AAGGACTGTG TGCTACAAAA 
CTGAGTGCCA ATTCAGAGGA CATTACTGAC AGACTGGGCA GCATTTCCGT AGGACTTCCC 
AGTTCAACAA TGGAGCAACC AAAGCCAATG GTTCAAACAA AAGGCAGACC GCACAGTCAG 
TGTTTGAACT CCTCTCCTTT ATCTCATCAT TCCCCGTTAA TGTTTCCACC CTTGTCAACT 
CCTCCTTCAT CTGCCCCATC TGCACCACCT GGCACTGGAA CAGATGTCTC TAAGCATAGA 
CCTCAGGGAT TCACTCCCTG CAAAATACCT TCTGCATCTC CTCAAACACA GCGCAAGTTT 
TCTCTACAGT TCCAGAGAAA CTGTTCCGAA AACAGAGACT CAGATAAACT TTCCCCCATT 
TTTACTCAAT CAAGACCCCT GCCCTCTAGT AACATACACA GGCCAAAGCC ATCTCGACCT 
ACCCCAGGTA ATACAAGTAA ACAGGGAGAT GCCTCAAAGA ATAGCATGAC ACTTGATCTG 
AACAGTACTT CCAAATGTGA TGACAGCTTT GGCAGCAGCA GCAATAGTAG TAATGCTCTT 
ATACCCAGTG ATGAGACAGT GTTCACCCCA GTAGAAGAGA AATGCAGGTT AGATGTCAAC 
ACAGAACTCA ACTCCAGTAT TGAGGACCTT CTTGAAGCAT CCATGCCTTC AAGTGACACG 
ACAGTAACTT TTAAGTCAGA AGTTGCTGTC CTCTCTCCTG AAAAGGATGA AAATGATGAT 
ACCTACAAAG ATGATGTGAA TCATAATCAG AAATGCAAAG AAAAGATGGA AGCTGAAGAA 
GAAGAAGCTT TAGCAATTGC CATGGCAATG TCAGCGTCTC AGGATGCCCT CCCCATAGTT 
CCTCAACTAC AGGTTGAAAA TGGAGAAGAT ATCATCATTA TTCAACAGGA TACACCGGAA 
ACTCTACCAG GACATACCAA AGCAAAACAA CCTTACAGAG AAGACACTGA ATGGCTGAAA 
GGTCAGCAGA TAGGCCTTGG AGCATTTTCT TCTTGTTACC AGGCTCAGGA TGTGGGAACT 
GGAACTTTAA TGGCTGTTAA ACAGGTGACA TATGTCAGAA ATACATCTTC TGAACAAGAA 
GAAGTAGTGG AAGCATTAAG AGAAGAGATC AGAATGATGA GCCATTTGAA TCATCCCAAC 
ATCATTAGGA TGTTGGGAGC CACATGTGAG AAGAGCAATT ATAATCTCTT CATTGAATGG 
ATGGCAGGGG GATCTGTGGC TCATTTGCTC AGTAAATACG GGGCCTTCAA GGAATCAGTA 
GTTATTAACT ATACTGAACA GTTACTTCGT GGCCTTTCCT ATCTCCATGA AAACCAGATC 
ATTCATAGAG ATGTCAAAGG TGCCAATTTG CTAATTGACA GCACAGGTCA GAGACTGAGA 
ATTGCAGATT TTGGAGCTGC AGCCAGGTTG GCATCAAAAG GAACTGGTGC AGGAGAGTTT 
CAGGGACAGT TATTGGGAAC AATTGCATTT ATGGCACCTG AGGTACTGAG AGGTCAGCAG 
TATGGTAGGA GCTGTGATGT ATGGAGTGTT GGCTGTGCTA TTATAGAAAT GGCTTGTGCT 
AAACCACCTT GGAATGCAGA AAAACACTCC AATCATCTTG CTTTGATATT TAAGATTGCA 
AGTGCGACGA CTGCTCCATC AATCCCCTCA CATTTGTCTC CTGGTTTACG AGATGTGGCT 
CTTCGGTGTT TAGAACTTCA ACCTCAGGAC AGACCCCCGT CAAGAGAGCT ACTGAAGCAT 
CCGGTCTTCC GTACTACATG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_535240.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_535240.4 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.

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CloneID	OCe03115
Clone ID Related Accession (Same CDS sequence)	XM_535240.5 , XM_535240.6
Accession Version	XM_535240.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mitogen-activated protein kinase kinase kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_535240.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGGGGCGGCG 
AGCCCCGAGG CGGGCGGCGG CGGAGCGGCT CTCAAGGCGA GCAGCGCGCC CGCGGCAGCC 
GCGGGGCTGC TGCGGGAGGG GGGCAGCGGG GGCCGCGAGC GGGCTGACTG GCGGCGGCGG 
CAGCTGCGCA AAGTGCGGAG CGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC 
GCCGCCTCGC CGCCGTCCTC CTCCCCTTCC CCGTCGCCGG AGCCCTCGGA CGCCGCGGCG 
GGCGGGAGCG CCTTCCAGCC TCTGGCGGCG CCGCAGCCGC ACGGAGCCGC GAGCCGTGGC 
GGCGCCCGCC CTCCGGAGCC CGCGGCCGCC CCGGACAGCG GCGCCCCGAG CCCCGCGGGG 
GCCGAGCCCG GGGAGAAGCG GACGCCCGCC GCCGAGCCGC CTCCTGCAGC GGCCCCCGCC 
GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAGGGGTTGC ACAAGATGGA CGATCGCCCA 
GAAGAACGAA TGATTAGGGA GAAACTGAAG GCAACCTGTA TGCCTGCCTG GAAGCACGAG 
TGGCTGGAAA GGAGAAACAG GAGAGGCCCT GTGGTGGTAA AACCAATCCC TACTAAAGGA 
GATGGTTCTG AAATTCACAA TCTGGCTACT GAGTCTCAAG GAGAAGGCCA GGCAAGCACT 
GCATCACCCA CTGCTAAAGG CCGACGCAGT CCTTCTCCTG GCAGCTCCCC ATCTGGTCGC 
ACAGTGAAGT CCGAATCTCC GGGAGTTCGG AGAAAAAGAG TTTCCCCGGT GCCTTTTCAG 
AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC 
CCAGAGGAGA CAAACCGCCG TGTAAACAAA GTTATGAGAG CCAGGCTGTA CTTATTGCAA 
CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGACAGCC CGGACAATAA ATACCGGGTG 
TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTGTTT 
GTTATGCTCC GGGTATTTCA ACTGGAACCT TCAGATCCAA TGTTATGGAG AAAAACTTTA 
AAGAATTTTG AGGTTGAAAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 
AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 
TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAGGAGGAG 
CAGATGTGTC CTATTTGCTT GTTGGGCATG CTAGATGAAG AGAGCCTCAC GGTGTGTGAA 
GATGGCTGCA GAAACAAGCT GCATCACCAC TGCATGTCAA TCTGGGCAGA AGAATGTAGA 
AGAAATAGAG AGCCTTTAAT ATGTCCCCTT TGTAGGTCTA AGTGGAGATC TCACGATTTC 
TACAGCCATG AATTGTCAAG CCCTGTGGAT TCCCCTTCTT CCCTGCGAGC TGCACAGCAG 
CAAACCATAC AACAGCAGCC TTTGGCTGGA TCACAACGAA GGAGTCAGGA GAGCAATTTT 
AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA 
TGGATTCAGG TATTTGGAAT GGAACTTGTT GGCTGCTTAT TTTCTAAAAA CTGGAATGTA 
AGAGAGATGG CTCTCAGGCG TCTTTCACAC GATGTTAGTG GGGCTTTGCT CTTGGCAAAT 
GGGGAGAGCA CTGGAAACTC TGGGAGCAAC GGTAGAAGCA GTCCGAGTGC TGGAGCCAGC 
AGTGGGTCTT CCCAGACCAG TATCTCAGGA GACGTGGTAG AGGCATGCTG CAACGTTTTG 
TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACATTGAGA 
GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT 
CTCCGGCCAG TGGTAGACAC CATCCTAGTC AAGTGTGCAG ATGCCAATAG TCGTACAAGT 
CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGCC AAGCAGGAGA GTTGGCAGTT 
GGCAGAGAAA TACTTAAAGC TGGATCCATT GGTATTGGTG GCGTGGATTA CGTCTTAAGT 
TGTATTCTTG GAAACCAAAC TGAGTCAAAC AACTGGCAAG AACTTCTGGG CCGCCTTTGT 
CTTGTAGACA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACT 
GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAAA AGCTGCTGTC CCTCTTAACC 
TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCAATGGTTG GTAAACTCTC CCGAAGGATA 
TATTTGAGTT CTGCAAGAAT GGTTACTGCA GTACCCCACG TATTTTCAAA ACTATTAGAA 
ATGCTGAATG TTTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT 
GCAGATGAGG TGGAAATCGC TGAGGCCATC CATCTGGGCA TAGGAGACAC TTTGGATGAT 
CGACATGACA GCTTTTTGCA GGCATCCGTC CCCACCAGCT ATCCAGAAGC CACAGAGAAC 
AGCCCCCTTG AGCGTACAGA CCATTTAGAG AAAACTGGAA AAGGACTGTG TGCTACAAAA 
CTGAGTGCCA ATTCAGAGGA CATTACTGAC AGACTGGGCA GCATTTCCGT AGGACTTCCC 
AGTTCAACAA TGGAGCAACC AAAGCCAATG GTTCAAACAA AAGGCAGACC GCACAGTCAG 
TGTTTGAACT CCTCTCCTTT ATCTCATCAT TCCCCGTTAA TGTTTCCACC CTTGTCAACT 
CCTCCTTCAT CTGCCCCATC TGCACCACCT GGCACTGGAA CAGATGTCTC TAAGCATAGA 
CCTCAGGGAT TCACTCCCTG CAAAATACCT TCTGCATCTC CTCAAACACA GCGCAAGTTT 
TCTCTACAGT TCCAGAGAAA CTGTTCCGAA AACAGAGACT CAGATAAACT TTCCCCCATT 
TTTACTCAAT CAAGACCCCT GCCCTCTAGT AACATACACA GGCCAAAGCC ATCTCGACCT 
ACCCCAGGTA ATACAAGTAA ACAGGGAGAT GCCTCAAAGA ATAGCATGAC ACTTGATCTG 
AACAGTACTT CCAAATGTGA TGACAGCTTT GGCAGCAGCA GCAATAGTAG TAATGCTCTT 
ATACCCAGTG ATGAGACAGT GTTCACCCCA GTAGAAGAGA AATGCAGGTT AGATGTCAAC 
ACAGAACTCA ACTCCAGTAT TGAGGACCTT CTTGAAGCAT CCATGCCTTC AAGTGACACG 
ACAGTAACTT TTAAGTCAGA AGTTGCTGTC CTCTCTCCTG AAAAGGATGA AAATGATGAT 
ACCTACAAAG ATGATGTGAA TCATAATCAG AAATGCAAAG AAAAGATGGA AGCTGAAGAA 
GAAGAAGCTT TAGCAATTGC CATGGCAATG TCAGCGTCTC AGGATGCCCT CCCCATAGTT 
CCTCAACTAC AGGTTGAAAA TGGAGAAGAT ATCATCATTA TTCAACAGGA TACACCGGAA 
ACTCTACCAG GACATACCAA AGCAAAACAA CCTTACAGAG AAGACACTGA ATGGCTGAAA 
GGTCAGCAGA TAGGCCTTGG AGCATTTTCT TCTTGTTACC AGGCTCAGGA TGTGGGAACT 
GGAACTTTAA TGGCTGTTAA ACAGGTGACA TATGTCAGAA ATACATCTTC TGAACAAGAA 
GAAGTAGTGG AAGCATTAAG AGAAGAGATC AGAATGATGA GCCATTTGAA TCATCCCAAC 
ATCATTAGGA TGTTGGGAGC CACATGTGAG AAGAGCAATT ATAATCTCTT CATTGAATGG 
ATGGCAGGGG GATCTGTGGC TCATTTGCTC AGTAAATACG GGGCCTTCAA GGAATCAGTA 
GTTATTAACT ATACTGAACA GTTACTTCGT GGCCTTTCCT ATCTCCATGA AAACCAGATC 
ATTCATAGAG ATGTCAAAGG TGCCAATTTG CTAATTGACA GCACAGGTCA GAGACTGAGA 
ATTGCAGATT TTGGAGCTGC AGCCAGGTTG GCATCAAAAG GAACTGGTGC AGGAGAGTTT 
CAGGGACAGT TATTGGGAAC AATTGCATTT ATGGCACCTG AGGTACTGAG AGGTCAGCAG 
TATGGTAGGA GCTGTGATGT ATGGAGTGTT GGCTGTGCTA TTATAGAAAT GGCTTGTGCT 
AAACCACCTT GGAATGCAGA AAAACACTCC AATCATCTTG CTTTGATATT TAAGATTGCA 
AGTGCGACGA CTGCTCCATC AATCCCCTCA CATTTGTCTC CTGGTTTACG AGATGTGGCT 
CTTCGGTGTT TAGAACTTCA ACCTCAGGAC AGACCCCCGT CAAGAGAGCT ACTGAAGCAT 
CCGGTCTTCC GTACTACATG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_535240.4
CDS	19..4542
Translation	MAAAAGNRASSSGFPGAGAASPEAGGGGAALKASSAPAAAAGLLREGGSGGRERADWRRRQLRKVRSVELDQLPEPPLFLAASPPSSSPSPSPEPSDAAAGGSAFQPLAAPQPHGAASRGGARPPEPAAAPDSGAPSPAGAEPGEKRTPAAEPPPAAAPAGREMENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPTKGDGSEIHNLATESQGEGQASTASPTAKGRRSPSPGSSPSGRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTIQQQPLAGSQRRSQESNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSKNWNVREMALRRLSHDVSGALLLANGESTGNSGSNGRSSPSAGASSGSSQTSISGDVVEACCNVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLSCILGNQTESNNWQELLGRLCLVDRLLLEFPAEFYPHIVSTDVSQAEPVEVRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPHVFSKLLEMLNVSSSTHFTRMRRRLMAIADEVEIAEAIHLGIGDTLDDRHDSFLQASVPTSYPEATENSPLERTDHLEKTGKGLCATKLSANSEDITDRLGSISVGLPSSTMEQPKPMVQTKGRPHSQCLNSSPLSHHSPLMFPPLSTPPSSAPSAPPGTGTDVSKHRPQGFTPCKIPSASPQTQRKFSLQFQRNCSENRDSDKLSPIFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDASKNSMTLDLNSTSKCDDSFGSSSNSSNALIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKDENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_535240.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_535240.6

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGGGGCGGCG 
AGCCCCGAGG CGGGCGGCGG CGGAGCGGCT CTCAAGGCGA GCAGCGCGCC CGCGGCAGCC 
GCGGGGCTGC TGCGGGAGGG GGGCAGCGGG GGCCGCGAGC GGGCTGACTG GCGGCGGCGG 
CAGCTGCGCA AAGTGCGGAG CGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC 
GCCGCCTCGC CGCCGTCCTC CTCCCCTTCC CCGTCGCCGG AGCCCTCGGA CGCCGCGGCG 
GGCGGGAGCG CCTTCCAGCC TCTGGCGGCG CCGCAGCCGC ACGGAGCCGC GAGCCGTGGC 
GGCGCCCGCC CTCCGGAGCC CGCGGCCGCC CCGGACAGCG GCGCCCCGAG CCCCGCGGGG 
GCCGAGCCCG GGGAGAAGCG GACGCCCGCC GCCGAGCCGC CTCCTGCAGC GGCCCCCGCC 
GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAGGGGTTGC ACAAGATGGA CGATCGCCCA 
GAAGAACGAA TGATTAGGGA GAAACTGAAG GCAACCTGTA TGCCTGCCTG GAAGCACGAG 
TGGCTGGAAA GGAGAAACAG GAGAGGCCCT GTGGTGGTAA AACCAATCCC TACTAAAGGA 
GATGGTTCTG AAATTCACAA TCTGGCTACT GAGTCTCAAG GAGAAGGCCA GGCAAGCACT 
GCATCACCCA CTGCTAAAGG CCGACGCAGT CCTTCTCCTG GCAGCTCCCC ATCTGGTCGC 
ACAGTGAAGT CCGAATCTCC GGGAGTTCGG AGAAAAAGAG TTTCCCCGGT GCCTTTTCAG 
AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC 
CCAGAGGAGA CAAACCGCCG TGTAAACAAA GTTATGAGAG CCAGGCTGTA CTTATTGCAA 
CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGACAGCC CGGACAATAA ATACCGGGTG 
TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTGTTT 
GTTATGCTCC GGGTATTTCA ACTGGAACCT TCAGATCCAA TGTTATGGAG AAAAACTTTA 
AAGAATTTTG AGGTTGAAAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 
AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 
TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAGGAGGAG 
CAGATGTGTC CTATTTGCTT GTTGGGCATG CTAGATGAAG AGAGCCTCAC GGTGTGTGAA 
GATGGCTGCA GAAACAAGCT GCATCACCAC TGCATGTCAA TCTGGGCAGA AGAATGTAGA 
AGAAATAGAG AGCCTTTAAT ATGTCCCCTT TGTAGGTCTA AGTGGAGATC TCACGATTTC 
TACAGCCATG AATTGTCAAG CCCTGTGGAT TCCCCTTCTT CCCTGCGAGC TGCACAGCAG 
CAAACCATAC AACAGCAGCC TTTGGCTGGA TCACAACGAA GGAGTCAGGA GAGCAATTTT 
AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA 
TGGATTCAGG TATTTGGAAT GGAACTTGTT GGCTGCTTAT TTTCTAAAAA CTGGAATGTA 
AGAGAGATGG CTCTCAGGCG TCTTTCACAC GATGTTAGTG GGGCTTTGCT CTTGGCAAAT 
GGGGAGAGCA CTGGAAACTC TGGGAGCAAC GGTAGAAGCA GTCCGAGTGC TGGAGCCAGC 
AGTGGGTCTT CCCAGACCAG TATCTCAGGA GACGTGGTAG AGGCATGCTG CAACGTTTTG 
TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACATTGAGA 
GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT 
CTCCGGCCAG TGGTAGACAC CATCCTAGTC AAGTGTGCAG ATGCCAATAG TCGTACAAGT 
CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGCC AAGCAGGAGA GTTGGCAGTT 
GGCAGAGAAA TACTTAAAGC TGGATCCATT GGTATTGGTG GCGTGGATTA CGTCTTAAGT 
TGTATTCTTG GAAACCAAAC TGAGTCAAAC AACTGGCAAG AACTTCTGGG CCGCCTTTGT 
CTTGTAGACA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACT 
GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAAA AGCTGCTGTC CCTCTTAACC 
TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCAATGGTTG GTAAACTCTC CCGAAGGATA 
TATTTGAGTT CTGCAAGAAT GGTTACTGCA GTACCCCACG TATTTTCAAA ACTATTAGAA 
ATGCTGAATG TTTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT 
GCAGATGAGG TGGAAATCGC TGAGGCCATC CATCTGGGCA TAGGAGACAC TTTGGATGAT 
CGACATGACA GCTTTTTGCA GGCATCCGTC CCCACCAGCT ATCCAGAAGC CACAGAGAAC 
AGCCCCCTTG AGCGTACAGA CCATTTAGAG AAAACTGGAA AAGGACTGTG TGCTACAAAA 
CTGAGTGCCA ATTCAGAGGA CATTACTGAC AGACTGGGCA GCATTTCCGT AGGACTTCCC 
AGTTCAACAA TGGAGCAACC AAAGCCAATG GTTCAAACAA AAGGCAGACC GCACAGTCAG 
TGTTTGAACT CCTCTCCTTT ATCTCATCAT TCCCCGTTAA TGTTTCCACC CTTGTCAACT 
CCTCCTTCAT CTGCCCCATC TGCACCACCT GGCACTGGAA CAGATGTCTC TAAGCATAGA 
CCTCAGGGAT TCACTCCCTG CAAAATACCT TCTGCATCTC CTCAAACACA GCGCAAGTTT 
TCTCTACAGT TCCAGAGAAA CTGTTCCGAA AACAGAGACT CAGATAAACT TTCCCCCATT 
TTTACTCAAT CAAGACCCCT GCCCTCTAGT AACATACACA GGCCAAAGCC ATCTCGACCT 
ACCCCAGGTA ATACAAGTAA ACAGGGAGAT GCCTCAAAGA ATAGCATGAC ACTTGATCTG 
AACAGTACTT CCAAATGTGA TGACAGCTTT GGCAGCAGCA GCAATAGTAG TAATGCTCTT 
ATACCCAGTG ATGAGACAGT GTTCACCCCA GTAGAAGAGA AATGCAGGTT AGATGTCAAC 
ACAGAACTCA ACTCCAGTAT TGAGGACCTT CTTGAAGCAT CCATGCCTTC AAGTGACACG 
ACAGTAACTT TTAAGTCAGA AGTTGCTGTC CTCTCTCCTG AAAAGGATGA AAATGATGAT 
ACCTACAAAG ATGATGTGAA TCATAATCAG AAATGCAAAG AAAAGATGGA AGCTGAAGAA 
GAAGAAGCTT TAGCAATTGC CATGGCAATG TCAGCGTCTC AGGATGCCCT CCCCATAGTT 
CCTCAACTAC AGGTTGAAAA TGGAGAAGAT ATCATCATTA TTCAACAGGA TACACCGGAA 
ACTCTACCAG GACATACCAA AGCAAAACAA CCTTACAGAG AAGACACTGA ATGGCTGAAA 
GGTCAGCAGA TAGGCCTTGG AGCATTTTCT TCTTGTTACC AGGCTCAGGA TGTGGGAACT 
GGAACTTTAA TGGCTGTTAA ACAGGTGACA TATGTCAGAA ATACATCTTC TGAACAAGAA 
GAAGTAGTGG AAGCATTAAG AGAAGAGATC AGAATGATGA GCCATTTGAA TCATCCCAAC 
ATCATTAGGA TGTTGGGAGC CACATGTGAG AAGAGCAATT ATAATCTCTT CATTGAATGG 
ATGGCAGGGG GATCTGTGGC TCATTTGCTC AGTAAATACG GGGCCTTCAA GGAATCAGTA 
GTTATTAACT ATACTGAACA GTTACTTCGT GGCCTTTCCT ATCTCCATGA AAACCAGATC 
ATTCATAGAG ATGTCAAAGG TGCCAATTTG CTAATTGACA GCACAGGTCA GAGACTGAGA 
ATTGCAGATT TTGGAGCTGC AGCCAGGTTG GCATCAAAAG GAACTGGTGC AGGAGAGTTT 
CAGGGACAGT TATTGGGAAC AATTGCATTT ATGGCACCTG AGGTACTGAG AGGTCAGCAG 
TATGGTAGGA GCTGTGATGT ATGGAGTGTT GGCTGTGCTA TTATAGAAAT GGCTTGTGCT 
AAACCACCTT GGAATGCAGA AAAACACTCC AATCATCTTG CTTTGATATT TAAGATTGCA 
AGTGCGACGA CTGCTCCATC AATCCCCTCA CATTTGTCTC CTGGTTTACG AGATGTGGCT 
CTTCGGTGTT TAGAACTTCA ACCTCAGGAC AGACCCCCGT CAAGAGAGCT ACTGAAGCAT 
CCGGTCTTCC GTACTACATG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_005617403.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617460.1 Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X4, mRNA.

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CloneID	OCe03118
Clone ID Related Accession (Same CDS sequence)	XM_005617403.1
Accession Version	XM_005617403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGGGGCGGCG 
AGCCCCGAGG CGGGCGGCGG CGGAGCGGCT CTCAAGGCGA GCAGCGCGCC CGCGGCAGCC 
GCGGGGCTGC TGCGGGAGGG GGGCAGCGGG GGCCGCGAGC GGGCTGACTG GCGGCGGCGG 
CAGCTGCGCA AAGTGCGGAG CGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC 
GCCGCCTCGC CGCCGTCCTC CTCCCCTTCC CCGTCGCCGG AGCCCTCGGA CGCCGCGGCG 
GGCGGGAGCG CCTTCCAGCC TCTGGCGGCG CCGCAGCCGC ACGGAGCCGC GAGCCGTGGC 
GGCGCCCGCC CTCCGGAGCC CGCGGCCGCC CCGGACAGCG GCGCCCCGAG CCCCGCGGGG 
GCCGAGCCCG GGGAGAAGCG GACGCCCGCC GCCGAGCCGC CTCCTGCAGC GGCCCCCGCC 
GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAGGGGTTGC ACAAGATGGA CGATCGCCCA 
GAAGAACGAA TGATTAGGGA GAAACTGAAG GCAACCTGTA TGCCTGCCTG GAAGCACGAG 
TGGCTGGAAA GGAGAAACAG GAGAGGCCCT GTGGTGGTAA AACCAATCCC TACTAAAGGA 
GATGGTTCTG AAATTCACAA TCTGGCTACT GAGTCTCAAG GAGAAGGCCA GGCAAGCACT 
GCATCACCCA CTGCTAAAGG CCGACGCAGT CCTTCTCCTG GCAGCTCCCC ATCTGGTCGC 
ACAGTGAAGT CCGAATCTCC GGGAGTTCGG AGAAAAAGAG TTTCCCCGGT GCCTTTTCAG 
AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC 
CCAGAGGAGA CAAACCGCCG TGTAAACAAA GTTATGAGAG CCAGGCTGTA CTTATTGCAA 
CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGACAGCC CGGACAATAA ATACCGGGTG 
TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTGTTT 
GTTATGCTCC GGGTATTTCA ACTGGAACCT TCAGATCCAA TGTTATGGAG AAAAACTTTA 
AAGAATTTTG AGGTTGAAAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 
AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 
TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAGGAGGAG 
CAGATGTGTC CTATTTGCTT GTTGGGCATG CTAGATGAAG AGAGCCTCAC GGTGTGTGAA 
GATGGCTGCA GAAACAAGCT GCATCACCAC TGCATGTCAA TCTGGGCAGA AGAATGTAGA 
AGAAATAGAG AGCCTTTAAT ATGTCCCCTT TGTAGGTCTA AGTGGAGATC TCACGATTTC 
TACAGCCATG AATTGTCAAG CCCTGTGGAT TCCCCTTCTT CCCTGCGAGC TGCACAGCAG 
CAAACCATAC AACAGCAGCC TTTGGCTGGA TCACAACGAA GGAGTCAGGA GAGCAATTTT 
AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA 
TGGATTCAGG TATTTGGAAT GGAACTTGTT GGCTGCTTAT TTTCTAAAAA CTGGAATGTA 
AGAGAGATGG CTCTCAGGCG TCTTTCACAC GATGTTAGTG GGGCTTTGCT CTTGGCAAAT 
GGGGAGAGCA CTGGAAACTC TGGGAGCAAC GGTAGAAGCA GTCCGAGTGC TGGAGCCAGC 
AGTGGGTCTT CCCAGACCAG TATCTCAGGA GACGTGGTAG AGGCATGCTG CAACGTTTTG 
TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACATTGAGA 
GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT 
CTCCGGCCAG TGGTAGACAC CATCCTAGTC AAGTGTGCAG ATGCCAATAG TCGTACAAGT 
CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGCC AAGCAGGAGA GTTGGCAGTT 
GGCAGAGAAA TACTTAAAGC TGGATCCATT GGTATTGGTG GCGTGGATTA CGTCTTAAGT 
TGTATTCTTG GAAACCAAAC TGAGTCAAAC AACTGGCAAG AACTTCTGGG CCGCCTTTGT 
CTTGTAGACA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACT 
GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAAA AGCTGCTGTC CCTCTTAACC 
TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCAATGGTTG GTAAACTCTC CCGAAGGATA 
TATTTGAGTT CTGCAAGAAT GGTTACTGCA GTACCCCACG TATTTTCAAA ACTATTAGAA 
ATGCTGAATG TTTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT 
GCAGATGAGG TGGAAATCGC TGAGGCCATC CATCTGGGCA TAGGAGACAC TTTGGATGAT 
CGACATGACA GCTTTTTGCA GGCATCCGTC CCCACCAGCT ATCCAGAAGC CACAGAGAAC 
AGCCCCCTTG AGCGTACAGA CCATTTAGAG AAAACTGGAA AAGGACTGTG TGCTACAAAA 
CTGAGTGCCA ATTCAGAGGA CATTACTGAC AGACTGGGCA GCATTTCCGT AGGACTTCCC 
AGTTCAACAA TGGAGCAACC AAAGCCAATG GTTCAAACAA AAGGCAGACC GCACAGTCAG 
TGTTTGAACT CCTCTCCTTT ATCTCATCAT TCCCCGTTAA TGTTTCCACC CTTGTCAACT 
CCTCCTTCAT CTGCCCCATC TGCACCACCT GGCACTGGAA CAGATGTCTC TAAGCATAGA 
CCTCAGGGAT TCACTCCCTG CAAAATACCT TCTGCATCTC CTCAAACACA GCGCAAGTTT 
TCTCTACAGT TCCAGAGAAA CTGTTCCGAA AACAGAGACT CAGATAAACT TTCCCCCATT 
TTTACTCAAT CAAGACCCCT GCCCTCTAGT AACATACACA GGCCAAAGCC ATCTCGACCT 
ACCCCAGGTA ATACAAGTAA ACAGGGAGAT GCCTCAAAGA ATAGCATGAC ACTTGATCTG 
AACAGTACTT CCAAATGTGA TGACAGCTTT GGCAGCAGCA GCAATAGTAG TAATGCTCTT 
ATACCCAGTG ATGAGACAGT GTTCACCCCA GTAGAAGAGA AATGCAGGTT AGATGTCAAC 
ACAGAACTCA ACTCCAGTAT TGAGGACCTT CTTGAAGCAT CCATGCCTTC AAGTGACACG 
ACAGTAACTT TTAAGTCAGA AGTTGCTGTC CTCTCTCCTG AAAAGGATGA AAATGATGAT 
ACCTACAAAG ATGATGTGAA TCATAATCAG AAATGCAAAG AAAAGATGGA AGCTGAAGAA 
GAAGAAGCTT TAGCAATTGC CATGGCAATG TCAGCGTCTC AGGATGCCCT CCCCATAGTT 
CCTCAACTAC AGGTTGAAAA TGGAGAAGAT ATCATCATTA TTCAACAGGA TACACCGGAA 
ACTCTACCAG GACATACCAA AGCAAAACAA CCTTACAGAG AAGACACTGA ATGGCTGAAA 
GGTCAGCAGA TAGGCCTTGG AGCATTTTCT TCTTGTTACC AGGCTCAGGA TGTGGGAACT 
GGAACTTTAA TGGCTGTTAA ACAGATCAGA TTGTTAGTAA AAATTGTAAA TGGAAATTCA 
TCAGAATTCA AAACTTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617460.1
CDS	13..3873
Translation	MAAAAGNRASSSGFPGAGAASPEAGGGGAALKASSAPAAAAGLLREGGSGGRERADWRRRQLRKVRSVELDQLPEPPLFLAASPPSSSPSPSPEPSDAAAGGSAFQPLAAPQPHGAASRGGARPPEPAAAPDSGAPSPAGAEPGEKRTPAAEPPPAAAPAGREMENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPTKGDGSEIHNLATESQGEGQASTASPTAKGRRSPSPGSSPSGRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTIQQQPLAGSQRRSQESNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSKNWNVREMALRRLSHDVSGALLLANGESTGNSGSNGRSSPSAGASSGSSQTSISGDVVEACCNVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLSCILGNQTESNNWQELLGRLCLVDRLLLEFPAEFYPHIVSTDVSQAEPVEVRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPHVFSKLLEMLNVSSSTHFTRMRRRLMAIADEVEIAEAIHLGIGDTLDDRHDSFLQASVPTSYPEATENSPLERTDHLEKTGKGLCATKLSANSEDITDRLGSISVGLPSSTMEQPKPMVQTKGRPHSQCLNSSPLSHHSPLMFPPLSTPPSSAPSAPPGTGTDVSKHRPQGFTPCKIPSASPQTQRKFSLQFQRNCSENRDSDKLSPIFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDASKNSMTLDLNSTSKCDDSFGSSSNSSNALIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKDENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQIRLLVKIVNGNSSEFKTL

Target ORF information:

RefSeq Version	XM_005617403.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617403.1

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CGGGGCGGCG 
AGCCCCGAGG CGGGCGGCGG CGGAGCGGCT CTCAAGGCGA GCAGCGCGCC CGCGGCAGCC 
GCGGGGCTGC TGCGGGAGGG GGGCAGCGGG GGCCGCGAGC GGGCTGACTG GCGGCGGCGG 
CAGCTGCGCA AAGTGCGGAG CGTGGAGCTG GACCAGCTGC CGGAGCCGCC GCTCTTCCTC 
GCCGCCTCGC CGCCGTCCTC CTCCCCTTCC CCGTCGCCGG AGCCCTCGGA CGCCGCGGCG 
GGCGGGAGCG CCTTCCAGCC TCTGGCGGCG CCGCAGCCGC ACGGAGCCGC GAGCCGTGGC 
GGCGCCCGCC CTCCGGAGCC CGCGGCCGCC CCGGACAGCG GCGCCCCGAG CCCCGCGGGG 
GCCGAGCCCG GGGAGAAGCG GACGCCCGCC GCCGAGCCGC CTCCTGCAGC GGCCCCCGCC 
GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAGGGGTTGC ACAAGATGGA CGATCGCCCA 
GAAGAACGAA TGATTAGGGA GAAACTGAAG GCAACCTGTA TGCCTGCCTG GAAGCACGAG 
TGGCTGGAAA GGAGAAACAG GAGAGGCCCT GTGGTGGTAA AACCAATCCC TACTAAAGGA 
GATGGTTCTG AAATTCACAA TCTGGCTACT GAGTCTCAAG GAGAAGGCCA GGCAAGCACT 
GCATCACCCA CTGCTAAAGG CCGACGCAGT CCTTCTCCTG GCAGCTCCCC ATCTGGTCGC 
ACAGTGAAGT CCGAATCTCC GGGAGTTCGG AGAAAAAGAG TTTCCCCGGT GCCTTTTCAG 
AGTGGGAGAA TCACACCACC TCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC 
CCAGAGGAGA CAAACCGCCG TGTAAACAAA GTTATGAGAG CCAGGCTGTA CTTATTGCAA 
CAAATAGGAC CCAACTCTTT CCTGATTGGA GGAGACAGCC CGGACAATAA ATACCGGGTG 
TTTATTGGGC CTCAGAACTG CAGCTGTGGG CGTGGAACAT TTTGTATTCA TCTGTTGTTT 
GTTATGCTCC GGGTATTTCA ACTGGAACCT TCAGATCCAA TGTTATGGAG AAAAACTTTA 
AAGAATTTTG AGGTTGAAAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 
AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 
TTGTCATCAT CTAGTACTTC TACATCTAGT TCAGAAAACA GTATAAAAGA TGAGGAGGAG 
CAGATGTGTC CTATTTGCTT GTTGGGCATG CTAGATGAAG AGAGCCTCAC GGTGTGTGAA 
GATGGCTGCA GAAACAAGCT GCATCACCAC TGCATGTCAA TCTGGGCAGA AGAATGTAGA 
AGAAATAGAG AGCCTTTAAT ATGTCCCCTT TGTAGGTCTA AGTGGAGATC TCACGATTTC 
TACAGCCATG AATTGTCAAG CCCTGTGGAT TCCCCTTCTT CCCTGCGAGC TGCACAGCAG 
CAAACCATAC AACAGCAGCC TTTGGCTGGA TCACAACGAA GGAGTCAGGA GAGCAATTTT 
AACCTTACTC ATTATGGAAC TCAGCAGATT CCTCCTGCTT ATAAAGATTT AGCTGAGCCA 
TGGATTCAGG TATTTGGAAT GGAACTTGTT GGCTGCTTAT TTTCTAAAAA CTGGAATGTA 
AGAGAGATGG CTCTCAGGCG TCTTTCACAC GATGTTAGTG GGGCTTTGCT CTTGGCAAAT 
GGGGAGAGCA CTGGAAACTC TGGGAGCAAC GGTAGAAGCA GTCCGAGTGC TGGAGCCAGC 
AGTGGGTCTT CCCAGACCAG TATCTCAGGA GACGTGGTAG AGGCATGCTG CAACGTTTTG 
TCAATGGTCT GTGCTGACCC TGTCTACAAA GTGTACGTTG CTGCTTTAAA AACATTGAGA 
GCCATGCTAG TATATACTCC TTGCCATAGT TTGGCAGAAA GAATCAAACT GCAGAGACTT 
CTCCGGCCAG TGGTAGACAC CATCCTAGTC AAGTGTGCAG ATGCCAATAG TCGTACAAGT 
CAGCTGTCTA TATCAACACT GTTGGAATTA TGCAAAGGCC AAGCAGGAGA GTTGGCAGTT 
GGCAGAGAAA TACTTAAAGC TGGATCCATT GGTATTGGTG GCGTGGATTA CGTCTTAAGT 
TGTATTCTTG GAAACCAAAC TGAGTCAAAC AACTGGCAAG AACTTCTGGG CCGCCTTTGT 
CTTGTAGACA GACTGCTGTT GGAATTTCCT GCTGAATTTT ACCCTCATAT TGTCAGTACT 
GATGTTTCAC AAGCTGAGCC TGTTGAAGTC AGGTATAAAA AGCTGCTGTC CCTCTTAACC 
TTTGCTTTGC AGTCCATTGA TAATTCCCAC TCAATGGTTG GTAAACTCTC CCGAAGGATA 
TATTTGAGTT CTGCAAGAAT GGTTACTGCA GTACCCCACG TATTTTCAAA ACTATTAGAA 
ATGCTGAATG TTTCCAGTTC CACTCATTTT ACCAGGATGC GTCGTCGTTT GATGGCTATT 
GCAGATGAGG TGGAAATCGC TGAGGCCATC CATCTGGGCA TAGGAGACAC TTTGGATGAT 
CGACATGACA GCTTTTTGCA GGCATCCGTC CCCACCAGCT ATCCAGAAGC CACAGAGAAC 
AGCCCCCTTG AGCGTACAGA CCATTTAGAG AAAACTGGAA AAGGACTGTG TGCTACAAAA 
CTGAGTGCCA ATTCAGAGGA CATTACTGAC AGACTGGGCA GCATTTCCGT AGGACTTCCC 
AGTTCAACAA TGGAGCAACC AAAGCCAATG GTTCAAACAA AAGGCAGACC GCACAGTCAG 
TGTTTGAACT CCTCTCCTTT ATCTCATCAT TCCCCGTTAA TGTTTCCACC CTTGTCAACT 
CCTCCTTCAT CTGCCCCATC TGCACCACCT GGCACTGGAA CAGATGTCTC TAAGCATAGA 
CCTCAGGGAT TCACTCCCTG CAAAATACCT TCTGCATCTC CTCAAACACA GCGCAAGTTT 
TCTCTACAGT TCCAGAGAAA CTGTTCCGAA AACAGAGACT CAGATAAACT TTCCCCCATT 
TTTACTCAAT CAAGACCCCT GCCCTCTAGT AACATACACA GGCCAAAGCC ATCTCGACCT 
ACCCCAGGTA ATACAAGTAA ACAGGGAGAT GCCTCAAAGA ATAGCATGAC ACTTGATCTG 
AACAGTACTT CCAAATGTGA TGACAGCTTT GGCAGCAGCA GCAATAGTAG TAATGCTCTT 
ATACCCAGTG ATGAGACAGT GTTCACCCCA GTAGAAGAGA AATGCAGGTT AGATGTCAAC 
ACAGAACTCA ACTCCAGTAT TGAGGACCTT CTTGAAGCAT CCATGCCTTC AAGTGACACG 
ACAGTAACTT TTAAGTCAGA AGTTGCTGTC CTCTCTCCTG AAAAGGATGA AAATGATGAT 
ACCTACAAAG ATGATGTGAA TCATAATCAG AAATGCAAAG AAAAGATGGA AGCTGAAGAA 
GAAGAAGCTT TAGCAATTGC CATGGCAATG TCAGCGTCTC AGGATGCCCT CCCCATAGTT 
CCTCAACTAC AGGTTGAAAA TGGAGAAGAT ATCATCATTA TTCAACAGGA TACACCGGAA 
ACTCTACCAG GACATACCAA AGCAAAACAA CCTTACAGAG AAGACACTGA ATGGCTGAAA 
GGTCAGCAGA TAGGCCTTGG AGCATTTTCT TCTTGTTACC AGGCTCAGGA TGTGGGAACT 
GGAACTTTAA TGGCTGTTAA ACAGATCAGA TTGTTAGTAA AAATTGTAAA TGGAAATTCA 
TCAGAATTCA AAACTTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MAP3K1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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