NRAP gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014108945.1	XP_013964420.1	nebulin-related-anchoring protein isoform X1
XM_846880.4	XP_851973.1	nebulin-related-anchoring protein isoform X4
XM_859724.5	XP_864817.1	nebulin-related-anchoring protein isoform X6
XM_014108945.2	XP_013964420.1	nebulin-related-anchoring protein isoform X1
XM_846880.5	XP_851973.1	nebulin-related-anchoring protein isoform X4
XM_014108949.2	XP_013964424.1	nebulin-related-anchoring protein isoform X5
XM_859724.4	XP_864817.1	nebulin-related-anchoring protein isoform X6
XM_014108946.1	XP_013964421.1	nebulin-related-anchoring protein isoform X2
XM_014108947.1	XP_013964422.1	nebulin-related-anchoring protein isoform X3
XM_014108949.1	XP_013964424.1	nebulin-related-anchoring protein isoform X5
XM_014108946.2	XP_013964421.1	nebulin-related-anchoring protein isoform X2
XM_014108947.2	XP_013964422.1	nebulin-related-anchoring protein isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe28040	XM_846880.5 Latest version!	Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
OCe28041	XM_859724.5 Latest version!	Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
OCe43944	XM_014108945.2 Latest version!	Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe43945	XM_014108946.2 Latest version!	Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe43946	XM_014108947.2 Latest version!	Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe43947	XM_014108949.2 Latest version!	Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
View Old Accession Versions >>
OCe43944	XM_014108945.1	Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe28040	XM_846880.4	Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
OCe28041	XM_859724.4	Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
OCe43947	XM_014108949.1	Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe43945	XM_014108946.1	Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe43946	XM_014108947.1	Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

NRAP ( XM_846880.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_851973.1 Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe28040
Clone ID Related Accession (Same CDS sequence)	XM_846880.4 , XM_846880.5
Accession Version	XM_846880.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	nebulin-related-anchoring protein isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_846880.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGGAGTA TACAGAAGAC 
TATGAACAAC AGAGGGGCAA AGGGAGCTTT CCAGCCATGA TCACACCTGC CTATCAAAGA 
GCCAAGAAAG CTAACCAGCT GGCCAGCCAA GTGGAATACA AGAGAGGGCA TGACGAGCGC 
ATCTCCAGGT TTACCACGGT GGCGGATACC CCTGAGCTGC TACGGGCCCA GGCAGGGGGA 
CAGCTTCAAA GTGATGTGAG ATACACAGAA GACTATGAAC AACAGAGAGG AAAAGGCAGT 
TTCCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAGA GAGCCAATGA GCTGGCTAGC 
GATGTGAGGT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTAG TCCAGCTGCT 
GGAGCTGCAG GCACAAGGGA ATGCATGGAC CGATATGGCC AGGGTTACTT GGAGGAATAT 
CAGGAGCACA GAGGAAAAGG CAGCTTCCCC GCCATGATCA CCCCAGCTTA TCAGAATGCC 
AAGAAAGCCA ACGAACTCGC TAGTGACATA AAATACAGGC AGGACTTCAA TAAGATGAAA 
GGCCCTGCAC ATTATCACTC CCTCCCAGCC CAGGACAACT TGGTTCTCAA GCGGGCCCAG 
AGCGTGAACA AGCTTGTGAG CGAGGTGGAA TATAAGAAGG ACCTGGAAAG TAGTAAAGGT 
CACAGTATCA ACTACTGTGA GACGCCTCAA TTCAGGAACG TGAGCAAGAT CTCAAAATTC 
ACCAGTGATA ATAAATATAA AGAAAATTAC CAGAACCACA TGAGAGGCCA CTATGAAGGA 
GTTGGTATGG ACAGACGGAC TCTCCATGCT ATGAAGGTTG GAAGCTTGGC AAGCAATATT 
GCCTACAAGG CTGATTATAA ACACGATGTT GTGGACTACA ACTACCCAGC CACTCTTACT 
CCCTCTTACC AGACAACAAT GAAACTTGTC CCCTTGAAAG ATGTCAACTA CAGGCAAAGT 
ATCGACAAGT TGAAGTACAG CTCTGTGACC AACACCCCTC AGATTATCCA AGCCAAAATC 
AATGCCCAAC AACTGAGTCA TGTGAATTAT CGTGCTGACT ATGAGAAAAA TAAGTTGAAC 
TACACCTTGC CACAGGATGT ACCTCAGCTG GTGAAGGCCA AAACCAATGC TGAACTATTT 
AGTGAGGTTA AGTATAAAGA AGGCTGGGAG AAGGCCAAGG GGAAAGGATT TGAGATGAAG 
CTGGATGCTA TGTCTTTGCT GGCCGCCAAA GCTTCTGGAG AGCTTGCTAG CAATATTAAA 
TATAAAGAAG AGTATGAAAA AACGAAAGGC AAAGTCATGG GCACTACAGA CTCGAGGCTT 
CTGCACTCTC TGCAGGTGGC CAAGATGAGC AGTGAGGTTG AATACAAGAA AGGCTTTGAA 
GAGAGTAAGA CCCACTTTCA CCTACCCATG GATATGGTGC ACATCAAGCA TGCTAAGAAG 
GCCCAAGCTC TGGCCAGTGA CCTGGACTAC AGGAAAAAGC TGCATGAATA CACTGTACTG 
CCTGAAGATA TGAAGACTCA GTGGGCCAAG AAAGCCTATG GGCTCCAGAG TGAGCTGCAG 
TACAAGGCCG ACCTAGCATG GATGAAAGGA GTCGGGTGGC TGACGGAGGG GAGTCTCAAT 
TTGGAACAGG CCAAGAAGGC TGGCCAGCTG GCCAGCGAGA AAAACTACAG GCAGAGGGTG 
GATGAGCTGA AGTTCACCAG TGTGACCGAC AGCTCCCAGA TGGAGCACGC CAAGAAGAGC 
CAGGAGCTGC AGAGCGGGGT GGCCTACAAG GCAGGAAATG AGCAGTCTGT CCATCAGTAC 
AGCATCAGCA AAGATGAACC TCTCTTCTTA CAGGCCCGAG TCAATGCCGC CAATCTCAGT 
GAGAAACTGT ATAAGAGCAG CTGGGAAAAC CAGAAGGCAA AAGGTTTTGA GCTGCGTCTG 
GACTCCTTGG GCTTCCTGGC AGCCAAAGCC AACAGGGACC TGGCGAGCGA GGTGAAGTAC 
AAGGAAGATT ACGAGAGGTC CCGAGGTAAG CTCATTGGAG CGAAGGATGC CCAGGGGGAT 
TCACAAATGA GCCACTCGCT GCAAATGTCC AAGCTGCAGA GTGAACTCGA GTACAAGAAA 
GGGTTCGAGG ACACCAGATC CCAGTGCCAC GTCTCCCTGG ACATGATCCA CCTTGTGCAT 
GCCCGCAAGG CTCAGCATTT AGCCACAGAC ATAGGCTACA AGACGGCATC TCATCAGTTC 
ACCGCTTTGC CCACCGACAT GAAGGTGGAA TGGGCCAAGA AGGCATACGG TTTACAGAGT 
GATAACCAAT ACAGGGCAGA TGTGAAGTGG ATGAAAGGCA CGGGCTGGGT CGCCACCGGG 
TCATTAAATG TGGAGCAGGC GAAGAAGGCA GGAGAACTCA TTAGCGAGAA GAAGTACCGT 
CAGCATCCAG ATGCTTTGAA GTTTACCAGT ATTAAAGACA CTCCGGAGAT GGTCCAGGCC 
AGAATTAGTT ATACCCAAGC AGTGGATAGG TTATACAGGG AACAAGGAGA AAATATAAAG 
CATCATTACA CTCAGACAGC CGACCTCCCT GAAGTCCTGC TGGCCAAGCT GAATGCTATG 
AATATCAGTG AGACGCGTTA TAAGGAATCC TGGAGCAAAC TTCGGGACGG TGGCTATAAA 
CTGAGGTTGG ATGCCATTCC GTTCCAGGCA GCAAAGGCTT CTGGCGAAAT CATAAGTGAT 
TACAAATACA AAGAGGCATT TGAGAAAATG AAAGGGCAGA TGCTTGGCTC CCGGAGCTTG 
GAAGACGATA TCAGCCTTGC ACATTCAGTG TATGCGAACT CGCTGCAGAG CGAAGTGAAT 
TACAAGAAAG GCTTTGAGCT CTCAAAGGCA CACTTCCATC TGCCGTTGGA CATGGTAACC 
CTGGTGCATG CCAAGAAGGC TCAGAGCCTG GCCAGCGACC AGGACTACAG ACACCCGCTC 
CCCCAGTACA CATCCTTGGC TGAAGACCTG AGGCTCAGCT GCGCCAAGAA AGCTCACAAG 
CTGCAGAGTG AGAATTTGTA CCGCTCAGAC CTGAACTTTA TGCGAGGCGT TCCCTGTGTC 
ATTCCGGGCA CATTAGAGAT TGAAGGGAGA AAGAAAGCAT CTGAACTCAT CAGTGAGAGT 
AAATACCGTC AGCATCCCCA GTCATTCAAG TACACAGCTG TGACAGACAC TCCCAGCCTC 
CTTCATGCAA AACTCAGCAA CCAGATTACC AATGAGCGCC TCTATAAAGC AGCCGGAGAG 
GATGAGAGGC ACCGGTACAC AATGACCCTG GGTCTGCCCG AGTTTGTCCG AGCGAAAGCG 
AACGCAGCCA ATCTGAGTGA CGCAAAATAC AAGGAGTCTT GGCGTAATCT CCGTGCTCAA 
GGCTACAAGC TGACAATAGA CGCTCTCCCC TTCCAGGCTG CTCGCGTCTC TGGAGATATA 
GCCAGTGATT TTCTCTACAG ACATGACTTT GTGAAGGAGC GAGGCAGGCT GATCGGGGCC 
CGGAGTGTGA GTGATGACCC CCGACTCCAG CACTGCTGGC GGGTAGGCCA GTTGCAGAGC 
GAGCTTCAGT ACAGGCGGGG AGCGGCGAGC AGCCAAGCCC ACTACCACCT GCCCATGGAC 
ATGGTGCACC TGGTCCATGC CAGGAAGGCC CAGGCCCTAG CCAGCGACCA CGACTACAGG 
ACACGGTGCC ATGAGTTCAC AGCTCTGCCT GAGGACCTGA AGATGTCCTG GGCCAAGAGA 
GCTCACGCCC TGCAGAGTGA GTTTCGCTAC AAGTCAGACC TGATGGGCAT GAAGGGGACA 
GGATGGCTGA CGCTGAGTTC GCCGCAGCTA GAGAGTGCAA AGAAGGCCGG AGAACTCATC 
AGCGAGACCA AATACCGTAA GAAACCAGAT AGTATCAAGT TCACCACGGT GGTTGACTCC 
CCAGACCTGG TTCACGCCAA GAACAGCTAC ATGCATTGCA ATGAGCGCCT GTACAGATTG 
GGGGATGCGG AATCCCTGCA CAGATATACG CTGATCCCCG ACCACCCCGA TTTCACCAGA 
GCCCGCCTCA ACGCGCTGCA TCTGAGTGAT AAAGTCTACA GAAACTCTTG GGAACAGACC 
CGGGCTGGTG GCTATGACTT CCGGCTGGAT GCCATCCCAT TCCAGACTGC CCGGGCGTCC 
CGGGAGATCG CCAGTGATTT CCGGTACAAA GAGGCCTTCC TGCGGGACCG GGGCCTACAG 
ATTGGTTACC GCAGCATCCA CGACGACCCC AGGATGAGGC ATTTCCTCAG CGTCGGCAAA 
CTCCAGAGTG ACAACGAGTA CAAGAAGGAC TTTGCCAAGA GTCGGTCCCA GTTCCATAGC 
CGCCCGGACC AGCCCGGCTT CCTCCAGGCC AGGAGGAGCC AGCAGCTGGC CAGCGACGTG 
TGCTACCGGC AGCCCCTGCC CCAGCCCACA TGCGACCCGG AGCAGCTAGG CCTGAAGCAC 
GCACAGAAGG CCCACCAGCT GCAGAGTGAC GTCAAGTACA AATCAGACTT GAACCTGACC 
AGAGGCGTTG GCTGGACACC CCCCGGCTCC TACAAAGTGG AAATGGCTCG GCGGGCGGCA 
GAACTGGCCA AGGCGAGGGG CCTGGGTCTC CGGGGCGCTT GGGGGGGGCC AGAGGCTGTG 
GAGCCCGCAG AGAATCAGAG GGGGGACGTG AACCCAGACG CCACCGAGAT TCTGCACGTC 
AAAAGGAGGA AGGCTCTGCC GTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_851973.1
CDS	203..5389
Translation	MNVQACSRCGYGVYPAEKISCLDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRKSPETIHGIDEQDEGERFKSVFHWDMKSRDGTAAPNRQPLMNERAYWNGYGEGNAWCPGAMPDPEIVRMVETRKSLGEEYTEDYEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKRGHDERISRFTTVADTPELLRAQAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMKGMASPAAGAAGTRECMDRYGQGYLEEYQEHRGKGSFPAMITPAYQNAKKANELASDIKYRQDFNKMKGPAHYHSLPAQDNLVLKRAQSVNKLVSEVEYKKDLESSKGHSINYCETPQFRNVSKISKFTSDNKYKENYQNHMRGHYEGVGMDRRTLHAMKVGSLASNIAYKADYKHDVVDYNYPATLTPSYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIIQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAELFSEVKYKEGWEKAKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKVMGTTDSRLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVHIKHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLASEKNYRQRVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYSISKDEPLFLQARVNAANLSEKLYKSSWENQKAKGFELRLDSLGFLAAKANRDLASEVKYKEDYERSRGKLIGAKDAQGDSQMSHSLQMSKLQSELEYKKGFEDTRSQCHVSLDMIHLVHARKAQHLATDIGYKTASHQFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTADLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASGEIISDYKYKEAFEKMKGQMLGSRSLEDDISLAHSVYANSLQSEVNYKKGFELSKAHFHLPLDMVTLVHAKKAQSLASDQDYRHPLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVPCVIPGTLEIEGRKKASELISESKYRQHPQSFKYTAVTDTPSLLHAKLSNQITNERLYKAAGEDERHRYTMTLGLPEFVRAKANAANLSDAKYKESWRNLRAQGYKLTIDALPFQAARVSGDIASDFLYRHDFVKERGRLIGARSVSDDPRLQHCWRVGQLQSELQYRRGAASSQAHYHLPMDMVHLVHARKAQALASDHDYRTRCHEFTALPEDLKMSWAKRAHALQSEFRYKSDLMGMKGTGWLTLSSPQLESAKKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERLYRLGDAESLHRYTLIPDHPDFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGYRSIHDDPRMRHFLSVGKLQSDNEYKKDFAKSRSQFHSRPDQPGFLQARRSQQLASDVCYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELAKARGLGLRGAWGGPEAVEPAENQRGDVNPDATEILHVKRRKALPL

Target ORF information:

RefSeq Version	XM_846880.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_846880.4

ORF Insert Sequence:

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGGAGTA TACAGAAGAC 
TATGAACAAC AGAGGGGCAA AGGGAGCTTT CCAGCCATGA TCACACCTGC CTATCAAAGA 
GCCAAGAAAG CTAACCAGCT GGCCAGCCAA GTGGAATACA AGAGAGGGCA TGACGAGCGC 
ATCTCCAGGT TTACCACGGT GGCGGATACC CCTGAGCTGC TACGGGCCCA GGCAGGGGGA 
CAGCTTCAAA GTGATGTGAG ATACACAGAA GACTATGAAC AACAGAGAGG AAAAGGCAGT 
TTCCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAGA GAGCCAATGA GCTGGCTAGC 
GATGTGAGGT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTAG TCCAGCTGCT 
GGAGCTGCAG GCACAAGGGA ATGCATGGAC CGATATGGCC AGGGTTACTT GGAGGAATAT 
CAGGAGCACA GAGGAAAAGG CAGCTTCCCC GCCATGATCA CCCCAGCTTA TCAGAATGCC 
AAGAAAGCCA ACGAACTCGC TAGTGACATA AAATACAGGC AGGACTTCAA TAAGATGAAA 
GGCCCTGCAC ATTATCACTC CCTCCCAGCC CAGGACAACT TGGTTCTCAA GCGGGCCCAG 
AGCGTGAACA AGCTTGTGAG CGAGGTGGAA TATAAGAAGG ACCTGGAAAG TAGTAAAGGT 
CACAGTATCA ACTACTGTGA GACGCCTCAA TTCAGGAACG TGAGCAAGAT CTCAAAATTC 
ACCAGTGATA ATAAATATAA AGAAAATTAC CAGAACCACA TGAGAGGCCA CTATGAAGGA 
GTTGGTATGG ACAGACGGAC TCTCCATGCT ATGAAGGTTG GAAGCTTGGC AAGCAATATT 
GCCTACAAGG CTGATTATAA ACACGATGTT GTGGACTACA ACTACCCAGC CACTCTTACT 
CCCTCTTACC AGACAACAAT GAAACTTGTC CCCTTGAAAG ATGTCAACTA CAGGCAAAGT 
ATCGACAAGT TGAAGTACAG CTCTGTGACC AACACCCCTC AGATTATCCA AGCCAAAATC 
AATGCCCAAC AACTGAGTCA TGTGAATTAT CGTGCTGACT ATGAGAAAAA TAAGTTGAAC 
TACACCTTGC CACAGGATGT ACCTCAGCTG GTGAAGGCCA AAACCAATGC TGAACTATTT 
AGTGAGGTTA AGTATAAAGA AGGCTGGGAG AAGGCCAAGG GGAAAGGATT TGAGATGAAG 
CTGGATGCTA TGTCTTTGCT GGCCGCCAAA GCTTCTGGAG AGCTTGCTAG CAATATTAAA 
TATAAAGAAG AGTATGAAAA AACGAAAGGC AAAGTCATGG GCACTACAGA CTCGAGGCTT 
CTGCACTCTC TGCAGGTGGC CAAGATGAGC AGTGAGGTTG AATACAAGAA AGGCTTTGAA 
GAGAGTAAGA CCCACTTTCA CCTACCCATG GATATGGTGC ACATCAAGCA TGCTAAGAAG 
GCCCAAGCTC TGGCCAGTGA CCTGGACTAC AGGAAAAAGC TGCATGAATA CACTGTACTG 
CCTGAAGATA TGAAGACTCA GTGGGCCAAG AAAGCCTATG GGCTCCAGAG TGAGCTGCAG 
TACAAGGCCG ACCTAGCATG GATGAAAGGA GTCGGGTGGC TGACGGAGGG GAGTCTCAAT 
TTGGAACAGG CCAAGAAGGC TGGCCAGCTG GCCAGCGAGA AAAACTACAG GCAGAGGGTG 
GATGAGCTGA AGTTCACCAG TGTGACCGAC AGCTCCCAGA TGGAGCACGC CAAGAAGAGC 
CAGGAGCTGC AGAGCGGGGT GGCCTACAAG GCAGGAAATG AGCAGTCTGT CCATCAGTAC 
AGCATCAGCA AAGATGAACC TCTCTTCTTA CAGGCCCGAG TCAATGCCGC CAATCTCAGT 
GAGAAACTGT ATAAGAGCAG CTGGGAAAAC CAGAAGGCAA AAGGTTTTGA GCTGCGTCTG 
GACTCCTTGG GCTTCCTGGC AGCCAAAGCC AACAGGGACC TGGCGAGCGA GGTGAAGTAC 
AAGGAAGATT ACGAGAGGTC CCGAGGTAAG CTCATTGGAG CGAAGGATGC CCAGGGGGAT 
TCACAAATGA GCCACTCGCT GCAAATGTCC AAGCTGCAGA GTGAACTCGA GTACAAGAAA 
GGGTTCGAGG ACACCAGATC CCAGTGCCAC GTCTCCCTGG ACATGATCCA CCTTGTGCAT 
GCCCGCAAGG CTCAGCATTT AGCCACAGAC ATAGGCTACA AGACGGCATC TCATCAGTTC 
ACCGCTTTGC CCACCGACAT GAAGGTGGAA TGGGCCAAGA AGGCATACGG TTTACAGAGT 
GATAACCAAT ACAGGGCAGA TGTGAAGTGG ATGAAAGGCA CGGGCTGGGT CGCCACCGGG 
TCATTAAATG TGGAGCAGGC GAAGAAGGCA GGAGAACTCA TTAGCGAGAA GAAGTACCGT 
CAGCATCCAG ATGCTTTGAA GTTTACCAGT ATTAAAGACA CTCCGGAGAT GGTCCAGGCC 
AGAATTAGTT ATACCCAAGC AGTGGATAGG TTATACAGGG AACAAGGAGA AAATATAAAG 
CATCATTACA CTCAGACAGC CGACCTCCCT GAAGTCCTGC TGGCCAAGCT GAATGCTATG 
AATATCAGTG AGACGCGTTA TAAGGAATCC TGGAGCAAAC TTCGGGACGG TGGCTATAAA 
CTGAGGTTGG ATGCCATTCC GTTCCAGGCA GCAAAGGCTT CTGGCGAAAT CATAAGTGAT 
TACAAATACA AAGAGGCATT TGAGAAAATG AAAGGGCAGA TGCTTGGCTC CCGGAGCTTG 
GAAGACGATA TCAGCCTTGC ACATTCAGTG TATGCGAACT CGCTGCAGAG CGAAGTGAAT 
TACAAGAAAG GCTTTGAGCT CTCAAAGGCA CACTTCCATC TGCCGTTGGA CATGGTAACC 
CTGGTGCATG CCAAGAAGGC TCAGAGCCTG GCCAGCGACC AGGACTACAG ACACCCGCTC 
CCCCAGTACA CATCCTTGGC TGAAGACCTG AGGCTCAGCT GCGCCAAGAA AGCTCACAAG 
CTGCAGAGTG AGAATTTGTA CCGCTCAGAC CTGAACTTTA TGCGAGGCGT TCCCTGTGTC 
ATTCCGGGCA CATTAGAGAT TGAAGGGAGA AAGAAAGCAT CTGAACTCAT CAGTGAGAGT 
AAATACCGTC AGCATCCCCA GTCATTCAAG TACACAGCTG TGACAGACAC TCCCAGCCTC 
CTTCATGCAA AACTCAGCAA CCAGATTACC AATGAGCGCC TCTATAAAGC AGCCGGAGAG 
GATGAGAGGC ACCGGTACAC AATGACCCTG GGTCTGCCCG AGTTTGTCCG AGCGAAAGCG 
AACGCAGCCA ATCTGAGTGA CGCAAAATAC AAGGAGTCTT GGCGTAATCT CCGTGCTCAA 
GGCTACAAGC TGACAATAGA CGCTCTCCCC TTCCAGGCTG CTCGCGTCTC TGGAGATATA 
GCCAGTGATT TTCTCTACAG ACATGACTTT GTGAAGGAGC GAGGCAGGCT GATCGGGGCC 
CGGAGTGTGA GTGATGACCC CCGACTCCAG CACTGCTGGC GGGTAGGCCA GTTGCAGAGC 
GAGCTTCAGT ACAGGCGGGG AGCGGCGAGC AGCCAAGCCC ACTACCACCT GCCCATGGAC 
ATGGTGCACC TGGTCCATGC CAGGAAGGCC CAGGCCCTAG CCAGCGACCA CGACTACAGG 
ACACGGTGCC ATGAGTTCAC AGCTCTGCCT GAGGACCTGA AGATGTCCTG GGCCAAGAGA 
GCTCACGCCC TGCAGAGTGA GTTTCGCTAC AAGTCAGACC TGATGGGCAT GAAGGGGACA 
GGATGGCTGA CGCTGAGTTC GCCGCAGCTA GAGAGTGCAA AGAAGGCCGG AGAACTCATC 
AGCGAGACCA AATACCGTAA GAAACCAGAT AGTATCAAGT TCACCACGGT GGTTGACTCC 
CCAGACCTGG TTCACGCCAA GAACAGCTAC ATGCATTGCA ATGAGCGCCT GTACAGATTG 
GGGGATGCGG AATCCCTGCA CAGATATACG CTGATCCCCG ACCACCCCGA TTTCACCAGA 
GCCCGCCTCA ACGCGCTGCA TCTGAGTGAT AAAGTCTACA GAAACTCTTG GGAACAGACC 
CGGGCTGGTG GCTATGACTT CCGGCTGGAT GCCATCCCAT TCCAGACTGC CCGGGCGTCC 
CGGGAGATCG CCAGTGATTT CCGGTACAAA GAGGCCTTCC TGCGGGACCG GGGCCTACAG 
ATTGGTTACC GCAGCATCCA CGACGACCCC AGGATGAGGC ATTTCCTCAG CGTCGGCAAA 
CTCCAGAGTG ACAACGAGTA CAAGAAGGAC TTTGCCAAGA GTCGGTCCCA GTTCCATAGC 
CGCCCGGACC AGCCCGGCTT CCTCCAGGCC AGGAGGAGCC AGCAGCTGGC CAGCGACGTG 
TGCTACCGGC AGCCCCTGCC CCAGCCCACA TGCGACCCGG AGCAGCTAGG CCTGAAGCAC 
GCACAGAAGG CCCACCAGCT GCAGAGTGAC GTCAAGTACA AATCAGACTT GAACCTGACC 
AGAGGCGTTG GCTGGACACC CCCCGGCTCC TACAAAGTGG AAATGGCTCG GCGGGCGGCA 
GAACTGGCCA AGGCGAGGGG CCTGGGTCTC CGGGGCGCTT GGGGGGGGCC AGAGGCTGTG 
GAGCCCGCAG AGAATCAGAG GGGGGACGTG AACCCAGACG CCACCGAGAT TCTGCACGTC 
AAAAGGAGGA AGGCTCTGCC GTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_846880.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_851973.1 Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe28040
Clone ID Related Accession (Same CDS sequence)	XM_846880.4 , XM_846880.5
Accession Version	XM_846880.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	nebulin-related-anchoring protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_846880.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGGAGTA TACAGAAGAC 
TATGAACAAC AGAGGGGCAA AGGGAGCTTT CCAGCCATGA TCACACCTGC CTATCAAAGA 
GCCAAGAAAG CTAACCAGCT GGCCAGCCAA GTGGAATACA AGAGAGGGCA TGACGAGCGC 
ATCTCCAGGT TTACCACGGT GGCGGATACC CCTGAGCTGC TACGGGCCCA GGCAGGGGGA 
CAGCTTCAAA GTGATGTGAG ATACACAGAA GACTATGAAC AACAGAGAGG AAAAGGCAGT 
TTCCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAGA GAGCCAATGA GCTGGCTAGC 
GATGTGAGGT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTAG TCCAGCTGCT 
GGAGCTGCAG GCACAAGGGA ATGCATGGAC CGATATGGCC AGGGTTACTT GGAGGAATAT 
CAGGAGCACA GAGGAAAAGG CAGCTTCCCC GCCATGATCA CCCCAGCTTA TCAGAATGCC 
AAGAAAGCCA ACGAACTCGC TAGTGACATA AAATACAGGC AGGACTTCAA TAAGATGAAA 
GGCCCTGCAC ATTATCACTC CCTCCCAGCC CAGGACAACT TGGTTCTCAA GCGGGCCCAG 
AGCGTGAACA AGCTTGTGAG CGAGGTGGAA TATAAGAAGG ACCTGGAAAG TAGTAAAGGT 
CACAGTATCA ACTACTGTGA GACGCCTCAA TTCAGGAACG TGAGCAAGAT CTCAAAATTC 
ACCAGTGATA ATAAATATAA AGAAAATTAC CAGAACCACA TGAGAGGCCA CTATGAAGGA 
GTTGGTATGG ACAGACGGAC TCTCCATGCT ATGAAGGTTG GAAGCTTGGC AAGCAATATT 
GCCTACAAGG CTGATTATAA ACACGATGTT GTGGACTACA ACTACCCAGC CACTCTTACT 
CCCTCTTACC AGACAACAAT GAAACTTGTC CCCTTGAAAG ATGTCAACTA CAGGCAAAGT 
ATCGACAAGT TGAAGTACAG CTCTGTGACC AACACCCCTC AGATTATCCA AGCCAAAATC 
AATGCCCAAC AACTGAGTCA TGTGAATTAT CGTGCTGACT ATGAGAAAAA TAAGTTGAAC 
TACACCTTGC CACAGGATGT ACCTCAGCTG GTGAAGGCCA AAACCAATGC TGAACTATTT 
AGTGAGGTTA AGTATAAAGA AGGCTGGGAG AAGGCCAAGG GGAAAGGATT TGAGATGAAG 
CTGGATGCTA TGTCTTTGCT GGCCGCCAAA GCTTCTGGAG AGCTTGCTAG CAATATTAAA 
TATAAAGAAG AGTATGAAAA AACGAAAGGC AAAGTCATGG GCACTACAGA CTCGAGGCTT 
CTGCACTCTC TGCAGGTGGC CAAGATGAGC AGTGAGGTTG AATACAAGAA AGGCTTTGAA 
GAGAGTAAGA CCCACTTTCA CCTACCCATG GATATGGTGC ACATCAAGCA TGCTAAGAAG 
GCCCAAGCTC TGGCCAGTGA CCTGGACTAC AGGAAAAAGC TGCATGAATA CACTGTACTG 
CCTGAAGATA TGAAGACTCA GTGGGCCAAG AAAGCCTATG GGCTCCAGAG TGAGCTGCAG 
TACAAGGCCG ACCTAGCATG GATGAAAGGA GTCGGGTGGC TGACGGAGGG GAGTCTCAAT 
TTGGAACAGG CCAAGAAGGC TGGCCAGCTG GCCAGCGAGA AAAACTACAG GCAGAGGGTG 
GATGAGCTGA AGTTCACCAG TGTGACCGAC AGCTCCCAGA TGGAGCACGC CAAGAAGAGC 
CAGGAGCTGC AGAGCGGGGT GGCCTACAAG GCAGGAAATG AGCAGTCTGT CCATCAGTAC 
AGCATCAGCA AAGATGAACC TCTCTTCTTA CAGGCCCGAG TCAATGCCGC CAATCTCAGT 
GAGAAACTGT ATAAGAGCAG CTGGGAAAAC CAGAAGGCAA AAGGTTTTGA GCTGCGTCTG 
GACTCCTTGG GCTTCCTGGC AGCCAAAGCC AACAGGGACC TGGCGAGCGA GGTGAAGTAC 
AAGGAAGATT ACGAGAGGTC CCGAGGTAAG CTCATTGGAG CGAAGGATGC CCAGGGGGAT 
TCACAAATGA GCCACTCGCT GCAAATGTCC AAGCTGCAGA GTGAACTCGA GTACAAGAAA 
GGGTTCGAGG ACACCAGATC CCAGTGCCAC GTCTCCCTGG ACATGATCCA CCTTGTGCAT 
GCCCGCAAGG CTCAGCATTT AGCCACAGAC ATAGGCTACA AGACGGCATC TCATCAGTTC 
ACCGCTTTGC CCACCGACAT GAAGGTGGAA TGGGCCAAGA AGGCATACGG TTTACAGAGT 
GATAACCAAT ACAGGGCAGA TGTGAAGTGG ATGAAAGGCA CGGGCTGGGT CGCCACCGGG 
TCATTAAATG TGGAGCAGGC GAAGAAGGCA GGAGAACTCA TTAGCGAGAA GAAGTACCGT 
CAGCATCCAG ATGCTTTGAA GTTTACCAGT ATTAAAGACA CTCCGGAGAT GGTCCAGGCC 
AGAATTAGTT ATACCCAAGC AGTGGATAGG TTATACAGGG AACAAGGAGA AAATATAAAG 
CATCATTACA CTCAGACAGC CGACCTCCCT GAAGTCCTGC TGGCCAAGCT GAATGCTATG 
AATATCAGTG AGACGCGTTA TAAGGAATCC TGGAGCAAAC TTCGGGACGG TGGCTATAAA 
CTGAGGTTGG ATGCCATTCC GTTCCAGGCA GCAAAGGCTT CTGGCGAAAT CATAAGTGAT 
TACAAATACA AAGAGGCATT TGAGAAAATG AAAGGGCAGA TGCTTGGCTC CCGGAGCTTG 
GAAGACGATA TCAGCCTTGC ACATTCAGTG TATGCGAACT CGCTGCAGAG CGAAGTGAAT 
TACAAGAAAG GCTTTGAGCT CTCAAAGGCA CACTTCCATC TGCCGTTGGA CATGGTAACC 
CTGGTGCATG CCAAGAAGGC TCAGAGCCTG GCCAGCGACC AGGACTACAG ACACCCGCTC 
CCCCAGTACA CATCCTTGGC TGAAGACCTG AGGCTCAGCT GCGCCAAGAA AGCTCACAAG 
CTGCAGAGTG AGAATTTGTA CCGCTCAGAC CTGAACTTTA TGCGAGGCGT TCCCTGTGTC 
ATTCCGGGCA CATTAGAGAT TGAAGGGAGA AAGAAAGCAT CTGAACTCAT CAGTGAGAGT 
AAATACCGTC AGCATCCCCA GTCATTCAAG TACACAGCTG TGACAGACAC TCCCAGCCTC 
CTTCATGCAA AACTCAGCAA CCAGATTACC AATGAGCGCC TCTATAAAGC AGCCGGAGAG 
GATGAGAGGC ACCGGTACAC AATGACCCTG GGTCTGCCCG AGTTTGTCCG AGCGAAAGCG 
AACGCAGCCA ATCTGAGTGA CGCAAAATAC AAGGAGTCTT GGCGTAATCT CCGTGCTCAA 
GGCTACAAGC TGACAATAGA CGCTCTCCCC TTCCAGGCTG CTCGCGTCTC TGGAGATATA 
GCCAGTGATT TTCTCTACAG ACATGACTTT GTGAAGGAGC GAGGCAGGCT GATCGGGGCC 
CGGAGTGTGA GTGATGACCC CCGACTCCAG CACTGCTGGC GGGTAGGCCA GTTGCAGAGC 
GAGCTTCAGT ACAGGCGGGG AGCGGCGAGC AGCCAAGCCC ACTACCACCT GCCCATGGAC 
ATGGTGCACC TGGTCCATGC CAGGAAGGCC CAGGCCCTAG CCAGCGACCA CGACTACAGG 
ACACGGTGCC ATGAGTTCAC AGCTCTGCCT GAGGACCTGA AGATGTCCTG GGCCAAGAGA 
GCTCACGCCC TGCAGAGTGA GTTTCGCTAC AAGTCAGACC TGATGGGCAT GAAGGGGACA 
GGATGGCTGA CGCTGAGTTC GCCGCAGCTA GAGAGTGCAA AGAAGGCCGG AGAACTCATC 
AGCGAGACCA AATACCGTAA GAAACCAGAT AGTATCAAGT TCACCACGGT GGTTGACTCC 
CCAGACCTGG TTCACGCCAA GAACAGCTAC ATGCATTGCA ATGAGCGCCT GTACAGATTG 
GGGGATGCGG AATCCCTGCA CAGATATACG CTGATCCCCG ACCACCCCGA TTTCACCAGA 
GCCCGCCTCA ACGCGCTGCA TCTGAGTGAT AAAGTCTACA GAAACTCTTG GGAACAGACC 
CGGGCTGGTG GCTATGACTT CCGGCTGGAT GCCATCCCAT TCCAGACTGC CCGGGCGTCC 
CGGGAGATCG CCAGTGATTT CCGGTACAAA GAGGCCTTCC TGCGGGACCG GGGCCTACAG 
ATTGGTTACC GCAGCATCCA CGACGACCCC AGGATGAGGC ATTTCCTCAG CGTCGGCAAA 
CTCCAGAGTG ACAACGAGTA CAAGAAGGAC TTTGCCAAGA GTCGGTCCCA GTTCCATAGC 
CGCCCGGACC AGCCCGGCTT CCTCCAGGCC AGGAGGAGCC AGCAGCTGGC CAGCGACGTG 
TGCTACCGGC AGCCCCTGCC CCAGCCCACA TGCGACCCGG AGCAGCTAGG CCTGAAGCAC 
GCACAGAAGG CCCACCAGCT GCAGAGTGAC GTCAAGTACA AATCAGACTT GAACCTGACC 
AGAGGCGTTG GCTGGACACC CCCCGGCTCC TACAAAGTGG AAATGGCTCG GCGGGCGGCA 
GAACTGGCCA AGGCGAGGGG CCTGGGTCTC CGGGGCGCTT GGGGGGGGCC AGAGGCTGTG 
GAGCCCGCAG AGAATCAGAG GGGGGACGTG AACCCAGACG CCACCGAGAT TCTGCACGTC 
AAAAGGAGGA AGGCTCTGCC GTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_851973.1
CDS	224..5410
Translation	MNVQACSRCGYGVYPAEKISCLDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRKSPETIHGIDEQDEGERFKSVFHWDMKSRDGTAAPNRQPLMNERAYWNGYGEGNAWCPGAMPDPEIVRMVETRKSLGEEYTEDYEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKRGHDERISRFTTVADTPELLRAQAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMKGMASPAAGAAGTRECMDRYGQGYLEEYQEHRGKGSFPAMITPAYQNAKKANELASDIKYRQDFNKMKGPAHYHSLPAQDNLVLKRAQSVNKLVSEVEYKKDLESSKGHSINYCETPQFRNVSKISKFTSDNKYKENYQNHMRGHYEGVGMDRRTLHAMKVGSLASNIAYKADYKHDVVDYNYPATLTPSYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIIQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAELFSEVKYKEGWEKAKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKVMGTTDSRLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVHIKHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLASEKNYRQRVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYSISKDEPLFLQARVNAANLSEKLYKSSWENQKAKGFELRLDSLGFLAAKANRDLASEVKYKEDYERSRGKLIGAKDAQGDSQMSHSLQMSKLQSELEYKKGFEDTRSQCHVSLDMIHLVHARKAQHLATDIGYKTASHQFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTADLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASGEIISDYKYKEAFEKMKGQMLGSRSLEDDISLAHSVYANSLQSEVNYKKGFELSKAHFHLPLDMVTLVHAKKAQSLASDQDYRHPLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVPCVIPGTLEIEGRKKASELISESKYRQHPQSFKYTAVTDTPSLLHAKLSNQITNERLYKAAGEDERHRYTMTLGLPEFVRAKANAANLSDAKYKESWRNLRAQGYKLTIDALPFQAARVSGDIASDFLYRHDFVKERGRLIGARSVSDDPRLQHCWRVGQLQSELQYRRGAASSQAHYHLPMDMVHLVHARKAQALASDHDYRTRCHEFTALPEDLKMSWAKRAHALQSEFRYKSDLMGMKGTGWLTLSSPQLESAKKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERLYRLGDAESLHRYTLIPDHPDFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGYRSIHDDPRMRHFLSVGKLQSDNEYKKDFAKSRSQFHSRPDQPGFLQARRSQQLASDVCYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELAKARGLGLRGAWGGPEAVEPAENQRGDVNPDATEILHVKRRKALPL

Target ORF information:

RefSeq Version	XM_846880.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_846880.5

ORF Insert Sequence:

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGGAGTA TACAGAAGAC 
TATGAACAAC AGAGGGGCAA AGGGAGCTTT CCAGCCATGA TCACACCTGC CTATCAAAGA 
GCCAAGAAAG CTAACCAGCT GGCCAGCCAA GTGGAATACA AGAGAGGGCA TGACGAGCGC 
ATCTCCAGGT TTACCACGGT GGCGGATACC CCTGAGCTGC TACGGGCCCA GGCAGGGGGA 
CAGCTTCAAA GTGATGTGAG ATACACAGAA GACTATGAAC AACAGAGAGG AAAAGGCAGT 
TTCCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAGA GAGCCAATGA GCTGGCTAGC 
GATGTGAGGT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTAG TCCAGCTGCT 
GGAGCTGCAG GCACAAGGGA ATGCATGGAC CGATATGGCC AGGGTTACTT GGAGGAATAT 
CAGGAGCACA GAGGAAAAGG CAGCTTCCCC GCCATGATCA CCCCAGCTTA TCAGAATGCC 
AAGAAAGCCA ACGAACTCGC TAGTGACATA AAATACAGGC AGGACTTCAA TAAGATGAAA 
GGCCCTGCAC ATTATCACTC CCTCCCAGCC CAGGACAACT TGGTTCTCAA GCGGGCCCAG 
AGCGTGAACA AGCTTGTGAG CGAGGTGGAA TATAAGAAGG ACCTGGAAAG TAGTAAAGGT 
CACAGTATCA ACTACTGTGA GACGCCTCAA TTCAGGAACG TGAGCAAGAT CTCAAAATTC 
ACCAGTGATA ATAAATATAA AGAAAATTAC CAGAACCACA TGAGAGGCCA CTATGAAGGA 
GTTGGTATGG ACAGACGGAC TCTCCATGCT ATGAAGGTTG GAAGCTTGGC AAGCAATATT 
GCCTACAAGG CTGATTATAA ACACGATGTT GTGGACTACA ACTACCCAGC CACTCTTACT 
CCCTCTTACC AGACAACAAT GAAACTTGTC CCCTTGAAAG ATGTCAACTA CAGGCAAAGT 
ATCGACAAGT TGAAGTACAG CTCTGTGACC AACACCCCTC AGATTATCCA AGCCAAAATC 
AATGCCCAAC AACTGAGTCA TGTGAATTAT CGTGCTGACT ATGAGAAAAA TAAGTTGAAC 
TACACCTTGC CACAGGATGT ACCTCAGCTG GTGAAGGCCA AAACCAATGC TGAACTATTT 
AGTGAGGTTA AGTATAAAGA AGGCTGGGAG AAGGCCAAGG GGAAAGGATT TGAGATGAAG 
CTGGATGCTA TGTCTTTGCT GGCCGCCAAA GCTTCTGGAG AGCTTGCTAG CAATATTAAA 
TATAAAGAAG AGTATGAAAA AACGAAAGGC AAAGTCATGG GCACTACAGA CTCGAGGCTT 
CTGCACTCTC TGCAGGTGGC CAAGATGAGC AGTGAGGTTG AATACAAGAA AGGCTTTGAA 
GAGAGTAAGA CCCACTTTCA CCTACCCATG GATATGGTGC ACATCAAGCA TGCTAAGAAG 
GCCCAAGCTC TGGCCAGTGA CCTGGACTAC AGGAAAAAGC TGCATGAATA CACTGTACTG 
CCTGAAGATA TGAAGACTCA GTGGGCCAAG AAAGCCTATG GGCTCCAGAG TGAGCTGCAG 
TACAAGGCCG ACCTAGCATG GATGAAAGGA GTCGGGTGGC TGACGGAGGG GAGTCTCAAT 
TTGGAACAGG CCAAGAAGGC TGGCCAGCTG GCCAGCGAGA AAAACTACAG GCAGAGGGTG 
GATGAGCTGA AGTTCACCAG TGTGACCGAC AGCTCCCAGA TGGAGCACGC CAAGAAGAGC 
CAGGAGCTGC AGAGCGGGGT GGCCTACAAG GCAGGAAATG AGCAGTCTGT CCATCAGTAC 
AGCATCAGCA AAGATGAACC TCTCTTCTTA CAGGCCCGAG TCAATGCCGC CAATCTCAGT 
GAGAAACTGT ATAAGAGCAG CTGGGAAAAC CAGAAGGCAA AAGGTTTTGA GCTGCGTCTG 
GACTCCTTGG GCTTCCTGGC AGCCAAAGCC AACAGGGACC TGGCGAGCGA GGTGAAGTAC 
AAGGAAGATT ACGAGAGGTC CCGAGGTAAG CTCATTGGAG CGAAGGATGC CCAGGGGGAT 
TCACAAATGA GCCACTCGCT GCAAATGTCC AAGCTGCAGA GTGAACTCGA GTACAAGAAA 
GGGTTCGAGG ACACCAGATC CCAGTGCCAC GTCTCCCTGG ACATGATCCA CCTTGTGCAT 
GCCCGCAAGG CTCAGCATTT AGCCACAGAC ATAGGCTACA AGACGGCATC TCATCAGTTC 
ACCGCTTTGC CCACCGACAT GAAGGTGGAA TGGGCCAAGA AGGCATACGG TTTACAGAGT 
GATAACCAAT ACAGGGCAGA TGTGAAGTGG ATGAAAGGCA CGGGCTGGGT CGCCACCGGG 
TCATTAAATG TGGAGCAGGC GAAGAAGGCA GGAGAACTCA TTAGCGAGAA GAAGTACCGT 
CAGCATCCAG ATGCTTTGAA GTTTACCAGT ATTAAAGACA CTCCGGAGAT GGTCCAGGCC 
AGAATTAGTT ATACCCAAGC AGTGGATAGG TTATACAGGG AACAAGGAGA AAATATAAAG 
CATCATTACA CTCAGACAGC CGACCTCCCT GAAGTCCTGC TGGCCAAGCT GAATGCTATG 
AATATCAGTG AGACGCGTTA TAAGGAATCC TGGAGCAAAC TTCGGGACGG TGGCTATAAA 
CTGAGGTTGG ATGCCATTCC GTTCCAGGCA GCAAAGGCTT CTGGCGAAAT CATAAGTGAT 
TACAAATACA AAGAGGCATT TGAGAAAATG AAAGGGCAGA TGCTTGGCTC CCGGAGCTTG 
GAAGACGATA TCAGCCTTGC ACATTCAGTG TATGCGAACT CGCTGCAGAG CGAAGTGAAT 
TACAAGAAAG GCTTTGAGCT CTCAAAGGCA CACTTCCATC TGCCGTTGGA CATGGTAACC 
CTGGTGCATG CCAAGAAGGC TCAGAGCCTG GCCAGCGACC AGGACTACAG ACACCCGCTC 
CCCCAGTACA CATCCTTGGC TGAAGACCTG AGGCTCAGCT GCGCCAAGAA AGCTCACAAG 
CTGCAGAGTG AGAATTTGTA CCGCTCAGAC CTGAACTTTA TGCGAGGCGT TCCCTGTGTC 
ATTCCGGGCA CATTAGAGAT TGAAGGGAGA AAGAAAGCAT CTGAACTCAT CAGTGAGAGT 
AAATACCGTC AGCATCCCCA GTCATTCAAG TACACAGCTG TGACAGACAC TCCCAGCCTC 
CTTCATGCAA AACTCAGCAA CCAGATTACC AATGAGCGCC TCTATAAAGC AGCCGGAGAG 
GATGAGAGGC ACCGGTACAC AATGACCCTG GGTCTGCCCG AGTTTGTCCG AGCGAAAGCG 
AACGCAGCCA ATCTGAGTGA CGCAAAATAC AAGGAGTCTT GGCGTAATCT CCGTGCTCAA 
GGCTACAAGC TGACAATAGA CGCTCTCCCC TTCCAGGCTG CTCGCGTCTC TGGAGATATA 
GCCAGTGATT TTCTCTACAG ACATGACTTT GTGAAGGAGC GAGGCAGGCT GATCGGGGCC 
CGGAGTGTGA GTGATGACCC CCGACTCCAG CACTGCTGGC GGGTAGGCCA GTTGCAGAGC 
GAGCTTCAGT ACAGGCGGGG AGCGGCGAGC AGCCAAGCCC ACTACCACCT GCCCATGGAC 
ATGGTGCACC TGGTCCATGC CAGGAAGGCC CAGGCCCTAG CCAGCGACCA CGACTACAGG 
ACACGGTGCC ATGAGTTCAC AGCTCTGCCT GAGGACCTGA AGATGTCCTG GGCCAAGAGA 
GCTCACGCCC TGCAGAGTGA GTTTCGCTAC AAGTCAGACC TGATGGGCAT GAAGGGGACA 
GGATGGCTGA CGCTGAGTTC GCCGCAGCTA GAGAGTGCAA AGAAGGCCGG AGAACTCATC 
AGCGAGACCA AATACCGTAA GAAACCAGAT AGTATCAAGT TCACCACGGT GGTTGACTCC 
CCAGACCTGG TTCACGCCAA GAACAGCTAC ATGCATTGCA ATGAGCGCCT GTACAGATTG 
GGGGATGCGG AATCCCTGCA CAGATATACG CTGATCCCCG ACCACCCCGA TTTCACCAGA 
GCCCGCCTCA ACGCGCTGCA TCTGAGTGAT AAAGTCTACA GAAACTCTTG GGAACAGACC 
CGGGCTGGTG GCTATGACTT CCGGCTGGAT GCCATCCCAT TCCAGACTGC CCGGGCGTCC 
CGGGAGATCG CCAGTGATTT CCGGTACAAA GAGGCCTTCC TGCGGGACCG GGGCCTACAG 
ATTGGTTACC GCAGCATCCA CGACGACCCC AGGATGAGGC ATTTCCTCAG CGTCGGCAAA 
CTCCAGAGTG ACAACGAGTA CAAGAAGGAC TTTGCCAAGA GTCGGTCCCA GTTCCATAGC 
CGCCCGGACC AGCCCGGCTT CCTCCAGGCC AGGAGGAGCC AGCAGCTGGC CAGCGACGTG 
TGCTACCGGC AGCCCCTGCC CCAGCCCACA TGCGACCCGG AGCAGCTAGG CCTGAAGCAC 
GCACAGAAGG CCCACCAGCT GCAGAGTGAC GTCAAGTACA AATCAGACTT GAACCTGACC 
AGAGGCGTTG GCTGGACACC CCCCGGCTCC TACAAAGTGG AAATGGCTCG GCGGGCGGCA 
GAACTGGCCA AGGCGAGGGG CCTGGGTCTC CGGGGCGCTT GGGGGGGGCC AGAGGCTGTG 
GAGCCCGCAG AGAATCAGAG GGGGGACGTG AACCCAGACG CCACCGAGAT TCTGCACGTC 
AAAAGGAGGA AGGCTCTGCC GTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_859724.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_864817.1 Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe28041
Clone ID Related Accession (Same CDS sequence)	XM_859724.5 , XM_859724.4
Accession Version	XM_859724.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	nebulin-related-anchoring protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_859724.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGGAGTA TACAGAAGAC 
TATGAACAAC AGAGGGGCAA AGGGAGCTTT CCAGCCATGA TCACACCTGC CTATCAAAGA 
GCCAAGAAAG CTAACCAGCT GGCCAGCCAA GTGGAATACA AGAGAGGGCA TGACGAGCGC 
ATCTCCAGGT TTACCACGGT GGCGGATACC CCTGAGCTGC TACGGGCCCA GGCAGGGGGA 
CAGCTTCAAA GTGATGTGAG ATACACAGAA GACTATGAAC AACAGAGAGG AAAAGGCAGT 
TTCCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAGA GAGCCAATGA GCTGGCTAGC 
GATGTGAGGT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTAG TCCAGCTGCT 
GGAGCTGCAG GCACAAGGGA ATGCATGGAC CGATATGGCC AGGGTTACTT GGAGGAATAT 
CAGGAGCACA GAGGAAAAGG CAGCTTCCCC GCCATGATCA CCCCAGCTTA TCAGAATGCC 
AAGAAAGCCA ACGAACTCGC TAGTGACATA AAATACAGGC AGGACTTCAA TAAGATGAAA 
GGCCCTGCAC ATTATCACTC CCTCCCAGCC CAGGACAACT TGGTTCTCAA GCGGGCCCAG 
AGCGTGAACA AGCTTGTGAG CGAGAATAAA TATAAAGAAA ATTACCAGAA CCACATGAGA 
GGCCACTATG AAGGAGTTGG TATGGACAGA CGGACTCTCC ATGCTATGAA GGTTGGAAGC 
TTGGCAAGCA ATATTGCCTA CAAGGCTGAT TATAAACACG ATGTTGTGGA CTACAACTAC 
CCAGCCACTC TTACTCCCTC TTACCAGACA ACAATGAAAC TTGTCCCCTT GAAAGATGTC 
AACTACAGGC AAAGTATCGA CAAGTTGAAG TACAGCTCTG TGACCAACAC CCCTCAGATT 
ATCCAAGCCA AAATCAATGC CCAACAACTG AGTCATGTGA ATTATCGTGC TGACTATGAG 
AAAAATAAGT TGAACTACAC CTTGCCACAG GATGTACCTC AGCTGGTGAA GGCCAAAACC 
AATGCTGAAC TATTTAGTGA GGTTAAGTAT AAAGAAGGCT GGGAGAAGGC CAAGGGGAAA 
GGATTTGAGA TGAAGCTGGA TGCTATGTCT TTGCTGGCCG CCAAAGCTTC TGGAGAGCTT 
GCTAGCAATA TTAAATATAA AGAAGAGTAT GAAAAAACGA AAGGCAAAGT CATGGGCACT 
ACAGACTCGA GGCTTCTGCA CTCTCTGCAG GTGGCCAAGA TGAGCAGTGA GGTTGAATAC 
AAGAAAGGCT TTGAAGAGAG TAAGACCCAC TTTCACCTAC CCATGGATAT GGTGCACATC 
AAGCATGCTA AGAAGGCCCA AGCTCTGGCC AGTGACCTGG ACTACAGGAA AAAGCTGCAT 
GAATACACTG TACTGCCTGA AGATATGAAG ACTCAGTGGG CCAAGAAAGC CTATGGGCTC 
CAGAGTGAGC TGCAGTACAA GGCCGACCTA GCATGGATGA AAGGAGTCGG GTGGCTGACG 
GAGGGGAGTC TCAATTTGGA ACAGGCCAAG AAGGCTGGCC AGCTGGCCAG CGAGAAAAAC 
TACAGGCAGA GGGTGGATGA GCTGAAGTTC ACCAGTGTGA CCGACAGCTC CCAGATGGAG 
CACGCCAAGA AGAGCCAGGA GCTGCAGAGC GGGGTGGCCT ACAAGGCAGG AAATGAGCAG 
TCTGTCCATC AGTACAGCAT CAGCAAAGAT GAACCTCTCT TCTTACAGGC CCGAGTCAAT 
GCCGCCAATC TCAGTGAGAA ACTGTATAAG AGCAGCTGGG AAAACCAGAA GGCAAAAGGT 
TTTGAGCTGC GTCTGGACTC CTTGGGCTTC CTGGCAGCCA AAGCCAACAG GGACCTGGCG 
AGCGAGGTGA AGTACAAGGA AGATTACGAG AGGTCCCGAG GTAAGCTCAT TGGAGCGAAG 
GATGCCCAGG GGGATTCACA AATGAGCCAC TCGCTGCAAA TGTCCAAGCT GCAGAGTGAA 
CTCGAGTACA AGAAAGGGTT CGAGGACACC AGATCCCAGT GCCACGTCTC CCTGGACATG 
ATCCACCTTG TGCATGCCCG CAAGGCTCAG CATTTAGCCA CAGACATAGG CTACAAGACG 
GCATCTCATC AGTTCACCGC TTTGCCCACC GACATGAAGG TGGAATGGGC CAAGAAGGCA 
TACGGTTTAC AGAGTGATAA CCAATACAGG GCAGATGTGA AGTGGATGAA AGGCACGGGC 
TGGGTCGCCA CCGGGTCATT AAATGTGGAG CAGGCGAAGA AGGCAGGAGA ACTCATTAGC 
GAGAAGAAGT ACCGTCAGCA TCCAGATGCT TTGAAGTTTA CCAGTATTAA AGACACTCCG 
GAGATGGTCC AGGCCAGAAT TAGTTATACC CAAGCAGTGG ATAGGTTATA CAGGGAACAA 
GGAGAAAATA TAAAGCATCA TTACACTCAG ACAGCCGACC TCCCTGAAGT CCTGCTGGCC 
AAGCTGAATG CTATGAATAT CAGTGAGACG CGTTATAAGG AATCCTGGAG CAAACTTCGG 
GACGGTGGCT ATAAACTGAG GTTGGATGCC ATTCCGTTCC AGGCAGCAAA GGCTTCTGGC 
GAAATCATAA GTGATTACAA ATACAAAGAG GCATTTGAGA AAATGAAAGG GCAGATGCTT 
GGCTCCCGGA GCTTGGAAGA CGATATCAGC CTTGCACATT CAGTGTATGC GAACTCGCTG 
CAGAGCGAAG TGAATTACAA GAAAGGCTTT GAGCTCTCAA AGGCACACTT CCATCTGCCG 
TTGGACATGG TAACCCTGGT GCATGCCAAG AAGGCTCAGA GCCTGGCCAG CGACCAGGAC 
TACAGACACC CGCTCCCCCA GTACACATCC TTGGCTGAAG ACCTGAGGCT CAGCTGCGCC 
AAGAAAGCTC ACAAGCTGCA GAGTGAGAAT TTGTACCGCT CAGACCTGAA CTTTATGCGA 
GGCGTTCCCT GTGTCATTCC GGGCACATTA GAGATTGAAG GGAGAAAGAA AGCATCTGAA 
CTCATCAGTG AGAGTAAATA CCGTCAGCAT CCCCAGTCAT TCAAGTACAC AGCTGTGACA 
GACACTCCCA GCCTCCTTCA TGCAAAACTC AGCAACCAGA TTACCAATGA GCGCCTCTAT 
AAAGCAGCCG GAGAGGATGA GAGGCACCGG TACACAATGA CCCTGGGTCT GCCCGAGTTT 
GTCCGAGCGA AAGCGAACGC AGCCAATCTG AGTGACGCAA AATACAAGGA GTCTTGGCGT 
AATCTCCGTG CTCAAGGCTA CAAGCTGACA ATAGACGCTC TCCCCTTCCA GGCTGCTCGC 
GTCTCTGGAG ATATAGCCAG TGATTTTCTC TACAGACATG ACTTTGTGAA GGAGCGAGGC 
AGGCTGATCG GGGCCCGGAG TGTGAGTGAT GACCCCCGAC TCCAGCACTG CTGGCGGGTA 
GGCCAGTTGC AGAGCGAGCT TCAGTACAGG CGGGGAGCGG CGAGCAGCCA AGCCCACTAC 
CACCTGCCCA TGGACATGGT GCACCTGGTC CATGCCAGGA AGGCCCAGGC CCTAGCCAGC 
GACCACGACT ACAGGACACG GTGCCATGAG TTCACAGCTC TGCCTGAGGA CCTGAAGATG 
TCCTGGGCCA AGAGAGCTCA CGCCCTGCAG AGTGAGTTTC GCTACAAGTC AGACCTGATG 
GGCATGAAGG GGACAGGATG GCTGACGCTG AGTTCGCCGC AGCTAGAGAG TGCAAAGAAG 
GCCGGAGAAC TCATCAGCGA GACCAAATAC CGTAAGAAAC CAGATAGTAT CAAGTTCACC 
ACGGTGGTTG ACTCCCCAGA CCTGGTTCAC GCCAAGAACA GCTACATGCA TTGCAATGAG 
CGCCTGTACA GATTGGGGGA TGCGGAATCC CTGCACAGAT ATACGCTGAT CCCCGACCAC 
CCCGATTTCA CCAGAGCCCG CCTCAACGCG CTGCATCTGA GTGATAAAGT CTACAGAAAC 
TCTTGGGAAC AGACCCGGGC TGGTGGCTAT GACTTCCGGC TGGATGCCAT CCCATTCCAG 
ACTGCCCGGG CGTCCCGGGA GATCGCCAGT GATTTCCGGT ACAAAGAGGC CTTCCTGCGG 
GACCGGGGCC TACAGATTGG TTACCGCAGC ATCCACGACG ACCCCAGGAT GAGGCATTTC 
CTCAGCGTCG GCAAACTCCA GAGTGACAAC GAGTACAAGA AGGACTTTGC CAAGAGTCGG 
TCCCAGTTCC ATAGCCGCCC GGACCAGCCC GGCTTCCTCC AGGCCAGGAG GAGCCAGCAG 
CTGGCCAGCG ACGTGTGCTA CCGGCAGCCC CTGCCCCAGC CCACATGCGA CCCGGAGCAG 
CTAGGCCTGA AGCACGCACA GAAGGCCCAC CAGCTGCAGA GTGACGTCAA GTACAAATCA 
GACTTGAACC TGACCAGAGG CGTTGGCTGG ACACCCCCCG GCTCCTACAA AGTGGAAATG 
GCTCGGCGGG CGGCAGAACT GGCCAAGGCG AGGGGCCTGG GTCTCCGGGG CGCTTGGGGG 
GGGCCAGAGG CTGTGGAGCC CGCAGAGAAT CAGAGGGGGG ACGTGAACCC AGACGCCACC 
GAGATTCTGC ACGTCAAAAG GAGGAAGGCT CTGCCGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_864817.1
CDS	224..5305
Translation	MNVQACSRCGYGVYPAEKISCLDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRKSPETIHGIDEQDEGERFKSVFHWDMKSRDGTAAPNRQPLMNERAYWNGYGEGNAWCPGAMPDPEIVRMVETRKSLGEEYTEDYEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKRGHDERISRFTTVADTPELLRAQAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMKGMASPAAGAAGTRECMDRYGQGYLEEYQEHRGKGSFPAMITPAYQNAKKANELASDIKYRQDFNKMKGPAHYHSLPAQDNLVLKRAQSVNKLVSENKYKENYQNHMRGHYEGVGMDRRTLHAMKVGSLASNIAYKADYKHDVVDYNYPATLTPSYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIIQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAELFSEVKYKEGWEKAKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKVMGTTDSRLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVHIKHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLASEKNYRQRVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYSISKDEPLFLQARVNAANLSEKLYKSSWENQKAKGFELRLDSLGFLAAKANRDLASEVKYKEDYERSRGKLIGAKDAQGDSQMSHSLQMSKLQSELEYKKGFEDTRSQCHVSLDMIHLVHARKAQHLATDIGYKTASHQFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTADLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASGEIISDYKYKEAFEKMKGQMLGSRSLEDDISLAHSVYANSLQSEVNYKKGFELSKAHFHLPLDMVTLVHAKKAQSLASDQDYRHPLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVPCVIPGTLEIEGRKKASELISESKYRQHPQSFKYTAVTDTPSLLHAKLSNQITNERLYKAAGEDERHRYTMTLGLPEFVRAKANAANLSDAKYKESWRNLRAQGYKLTIDALPFQAARVSGDIASDFLYRHDFVKERGRLIGARSVSDDPRLQHCWRVGQLQSELQYRRGAASSQAHYHLPMDMVHLVHARKAQALASDHDYRTRCHEFTALPEDLKMSWAKRAHALQSEFRYKSDLMGMKGTGWLTLSSPQLESAKKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERLYRLGDAESLHRYTLIPDHPDFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGYRSIHDDPRMRHFLSVGKLQSDNEYKKDFAKSRSQFHSRPDQPGFLQARRSQQLASDVCYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELAKARGLGLRGAWGGPEAVEPAENQRGDVNPDATEILHVKRRKALPL

Target ORF information:

RefSeq Version	XM_859724.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_859724.5

ORF Insert Sequence:

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGGAGTA TACAGAAGAC 
TATGAACAAC AGAGGGGCAA AGGGAGCTTT CCAGCCATGA TCACACCTGC CTATCAAAGA 
GCCAAGAAAG CTAACCAGCT GGCCAGCCAA GTGGAATACA AGAGAGGGCA TGACGAGCGC 
ATCTCCAGGT TTACCACGGT GGCGGATACC CCTGAGCTGC TACGGGCCCA GGCAGGGGGA 
CAGCTTCAAA GTGATGTGAG ATACACAGAA GACTATGAAC AACAGAGAGG AAAAGGCAGT 
TTCCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAGA GAGCCAATGA GCTGGCTAGC 
GATGTGAGGT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTAG TCCAGCTGCT 
GGAGCTGCAG GCACAAGGGA ATGCATGGAC CGATATGGCC AGGGTTACTT GGAGGAATAT 
CAGGAGCACA GAGGAAAAGG CAGCTTCCCC GCCATGATCA CCCCAGCTTA TCAGAATGCC 
AAGAAAGCCA ACGAACTCGC TAGTGACATA AAATACAGGC AGGACTTCAA TAAGATGAAA 
GGCCCTGCAC ATTATCACTC CCTCCCAGCC CAGGACAACT TGGTTCTCAA GCGGGCCCAG 
AGCGTGAACA AGCTTGTGAG CGAGAATAAA TATAAAGAAA ATTACCAGAA CCACATGAGA 
GGCCACTATG AAGGAGTTGG TATGGACAGA CGGACTCTCC ATGCTATGAA GGTTGGAAGC 
TTGGCAAGCA ATATTGCCTA CAAGGCTGAT TATAAACACG ATGTTGTGGA CTACAACTAC 
CCAGCCACTC TTACTCCCTC TTACCAGACA ACAATGAAAC TTGTCCCCTT GAAAGATGTC 
AACTACAGGC AAAGTATCGA CAAGTTGAAG TACAGCTCTG TGACCAACAC CCCTCAGATT 
ATCCAAGCCA AAATCAATGC CCAACAACTG AGTCATGTGA ATTATCGTGC TGACTATGAG 
AAAAATAAGT TGAACTACAC CTTGCCACAG GATGTACCTC AGCTGGTGAA GGCCAAAACC 
AATGCTGAAC TATTTAGTGA GGTTAAGTAT AAAGAAGGCT GGGAGAAGGC CAAGGGGAAA 
GGATTTGAGA TGAAGCTGGA TGCTATGTCT TTGCTGGCCG CCAAAGCTTC TGGAGAGCTT 
GCTAGCAATA TTAAATATAA AGAAGAGTAT GAAAAAACGA AAGGCAAAGT CATGGGCACT 
ACAGACTCGA GGCTTCTGCA CTCTCTGCAG GTGGCCAAGA TGAGCAGTGA GGTTGAATAC 
AAGAAAGGCT TTGAAGAGAG TAAGACCCAC TTTCACCTAC CCATGGATAT GGTGCACATC 
AAGCATGCTA AGAAGGCCCA AGCTCTGGCC AGTGACCTGG ACTACAGGAA AAAGCTGCAT 
GAATACACTG TACTGCCTGA AGATATGAAG ACTCAGTGGG CCAAGAAAGC CTATGGGCTC 
CAGAGTGAGC TGCAGTACAA GGCCGACCTA GCATGGATGA AAGGAGTCGG GTGGCTGACG 
GAGGGGAGTC TCAATTTGGA ACAGGCCAAG AAGGCTGGCC AGCTGGCCAG CGAGAAAAAC 
TACAGGCAGA GGGTGGATGA GCTGAAGTTC ACCAGTGTGA CCGACAGCTC CCAGATGGAG 
CACGCCAAGA AGAGCCAGGA GCTGCAGAGC GGGGTGGCCT ACAAGGCAGG AAATGAGCAG 
TCTGTCCATC AGTACAGCAT CAGCAAAGAT GAACCTCTCT TCTTACAGGC CCGAGTCAAT 
GCCGCCAATC TCAGTGAGAA ACTGTATAAG AGCAGCTGGG AAAACCAGAA GGCAAAAGGT 
TTTGAGCTGC GTCTGGACTC CTTGGGCTTC CTGGCAGCCA AAGCCAACAG GGACCTGGCG 
AGCGAGGTGA AGTACAAGGA AGATTACGAG AGGTCCCGAG GTAAGCTCAT TGGAGCGAAG 
GATGCCCAGG GGGATTCACA AATGAGCCAC TCGCTGCAAA TGTCCAAGCT GCAGAGTGAA 
CTCGAGTACA AGAAAGGGTT CGAGGACACC AGATCCCAGT GCCACGTCTC CCTGGACATG 
ATCCACCTTG TGCATGCCCG CAAGGCTCAG CATTTAGCCA CAGACATAGG CTACAAGACG 
GCATCTCATC AGTTCACCGC TTTGCCCACC GACATGAAGG TGGAATGGGC CAAGAAGGCA 
TACGGTTTAC AGAGTGATAA CCAATACAGG GCAGATGTGA AGTGGATGAA AGGCACGGGC 
TGGGTCGCCA CCGGGTCATT AAATGTGGAG CAGGCGAAGA AGGCAGGAGA ACTCATTAGC 
GAGAAGAAGT ACCGTCAGCA TCCAGATGCT TTGAAGTTTA CCAGTATTAA AGACACTCCG 
GAGATGGTCC AGGCCAGAAT TAGTTATACC CAAGCAGTGG ATAGGTTATA CAGGGAACAA 
GGAGAAAATA TAAAGCATCA TTACACTCAG ACAGCCGACC TCCCTGAAGT CCTGCTGGCC 
AAGCTGAATG CTATGAATAT CAGTGAGACG CGTTATAAGG AATCCTGGAG CAAACTTCGG 
GACGGTGGCT ATAAACTGAG GTTGGATGCC ATTCCGTTCC AGGCAGCAAA GGCTTCTGGC 
GAAATCATAA GTGATTACAA ATACAAAGAG GCATTTGAGA AAATGAAAGG GCAGATGCTT 
GGCTCCCGGA GCTTGGAAGA CGATATCAGC CTTGCACATT CAGTGTATGC GAACTCGCTG 
CAGAGCGAAG TGAATTACAA GAAAGGCTTT GAGCTCTCAA AGGCACACTT CCATCTGCCG 
TTGGACATGG TAACCCTGGT GCATGCCAAG AAGGCTCAGA GCCTGGCCAG CGACCAGGAC 
TACAGACACC CGCTCCCCCA GTACACATCC TTGGCTGAAG ACCTGAGGCT CAGCTGCGCC 
AAGAAAGCTC ACAAGCTGCA GAGTGAGAAT TTGTACCGCT CAGACCTGAA CTTTATGCGA 
GGCGTTCCCT GTGTCATTCC GGGCACATTA GAGATTGAAG GGAGAAAGAA AGCATCTGAA 
CTCATCAGTG AGAGTAAATA CCGTCAGCAT CCCCAGTCAT TCAAGTACAC AGCTGTGACA 
GACACTCCCA GCCTCCTTCA TGCAAAACTC AGCAACCAGA TTACCAATGA GCGCCTCTAT 
AAAGCAGCCG GAGAGGATGA GAGGCACCGG TACACAATGA CCCTGGGTCT GCCCGAGTTT 
GTCCGAGCGA AAGCGAACGC AGCCAATCTG AGTGACGCAA AATACAAGGA GTCTTGGCGT 
AATCTCCGTG CTCAAGGCTA CAAGCTGACA ATAGACGCTC TCCCCTTCCA GGCTGCTCGC 
GTCTCTGGAG ATATAGCCAG TGATTTTCTC TACAGACATG ACTTTGTGAA GGAGCGAGGC 
AGGCTGATCG GGGCCCGGAG TGTGAGTGAT GACCCCCGAC TCCAGCACTG CTGGCGGGTA 
GGCCAGTTGC AGAGCGAGCT TCAGTACAGG CGGGGAGCGG CGAGCAGCCA AGCCCACTAC 
CACCTGCCCA TGGACATGGT GCACCTGGTC CATGCCAGGA AGGCCCAGGC CCTAGCCAGC 
GACCACGACT ACAGGACACG GTGCCATGAG TTCACAGCTC TGCCTGAGGA CCTGAAGATG 
TCCTGGGCCA AGAGAGCTCA CGCCCTGCAG AGTGAGTTTC GCTACAAGTC AGACCTGATG 
GGCATGAAGG GGACAGGATG GCTGACGCTG AGTTCGCCGC AGCTAGAGAG TGCAAAGAAG 
GCCGGAGAAC TCATCAGCGA GACCAAATAC CGTAAGAAAC CAGATAGTAT CAAGTTCACC 
ACGGTGGTTG ACTCCCCAGA CCTGGTTCAC GCCAAGAACA GCTACATGCA TTGCAATGAG 
CGCCTGTACA GATTGGGGGA TGCGGAATCC CTGCACAGAT ATACGCTGAT CCCCGACCAC 
CCCGATTTCA CCAGAGCCCG CCTCAACGCG CTGCATCTGA GTGATAAAGT CTACAGAAAC 
TCTTGGGAAC AGACCCGGGC TGGTGGCTAT GACTTCCGGC TGGATGCCAT CCCATTCCAG 
ACTGCCCGGG CGTCCCGGGA GATCGCCAGT GATTTCCGGT ACAAAGAGGC CTTCCTGCGG 
GACCGGGGCC TACAGATTGG TTACCGCAGC ATCCACGACG ACCCCAGGAT GAGGCATTTC 
CTCAGCGTCG GCAAACTCCA GAGTGACAAC GAGTACAAGA AGGACTTTGC CAAGAGTCGG 
TCCCAGTTCC ATAGCCGCCC GGACCAGCCC GGCTTCCTCC AGGCCAGGAG GAGCCAGCAG 
CTGGCCAGCG ACGTGTGCTA CCGGCAGCCC CTGCCCCAGC CCACATGCGA CCCGGAGCAG 
CTAGGCCTGA AGCACGCACA GAAGGCCCAC CAGCTGCAGA GTGACGTCAA GTACAAATCA 
GACTTGAACC TGACCAGAGG CGTTGGCTGG ACACCCCCCG GCTCCTACAA AGTGGAAATG 
GCTCGGCGGG CGGCAGAACT GGCCAAGGCG AGGGGCCTGG GTCTCCGGGG CGCTTGGGGG 
GGGCCAGAGG CTGTGGAGCC CGCAGAGAAT CAGAGGGGGG ACGTGAACCC AGACGCCACC 
GAGATTCTGC ACGTCAAAAG GAGGAAGGCT CTGCCGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_859724.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_864817.1 Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X6, mRNA.

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CloneID	OCe28041
Clone ID Related Accession (Same CDS sequence)	XM_859724.5 , XM_859724.4
Accession Version	XM_859724.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	nebulin-related-anchoring protein isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_859724.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGGAGTA TACAGAAGAC 
TATGAACAAC AGAGGGGCAA AGGGAGCTTT CCAGCCATGA TCACACCTGC CTATCAAAGA 
GCCAAGAAAG CTAACCAGCT GGCCAGCCAA GTGGAATACA AGAGAGGGCA TGACGAGCGC 
ATCTCCAGGT TTACCACGGT GGCGGATACC CCTGAGCTGC TACGGGCCCA GGCAGGGGGA 
CAGCTTCAAA GTGATGTGAG ATACACAGAA GACTATGAAC AACAGAGAGG AAAAGGCAGT 
TTCCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAGA GAGCCAATGA GCTGGCTAGC 
GATGTGAGGT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTAG TCCAGCTGCT 
GGAGCTGCAG GCACAAGGGA ATGCATGGAC CGATATGGCC AGGGTTACTT GGAGGAATAT 
CAGGAGCACA GAGGAAAAGG CAGCTTCCCC GCCATGATCA CCCCAGCTTA TCAGAATGCC 
AAGAAAGCCA ACGAACTCGC TAGTGACATA AAATACAGGC AGGACTTCAA TAAGATGAAA 
GGCCCTGCAC ATTATCACTC CCTCCCAGCC CAGGACAACT TGGTTCTCAA GCGGGCCCAG 
AGCGTGAACA AGCTTGTGAG CGAGAATAAA TATAAAGAAA ATTACCAGAA CCACATGAGA 
GGCCACTATG AAGGAGTTGG TATGGACAGA CGGACTCTCC ATGCTATGAA GGTTGGAAGC 
TTGGCAAGCA ATATTGCCTA CAAGGCTGAT TATAAACACG ATGTTGTGGA CTACAACTAC 
CCAGCCACTC TTACTCCCTC TTACCAGACA ACAATGAAAC TTGTCCCCTT GAAAGATGTC 
AACTACAGGC AAAGTATCGA CAAGTTGAAG TACAGCTCTG TGACCAACAC CCCTCAGATT 
ATCCAAGCCA AAATCAATGC CCAACAACTG AGTCATGTGA ATTATCGTGC TGACTATGAG 
AAAAATAAGT TGAACTACAC CTTGCCACAG GATGTACCTC AGCTGGTGAA GGCCAAAACC 
AATGCTGAAC TATTTAGTGA GGTTAAGTAT AAAGAAGGCT GGGAGAAGGC CAAGGGGAAA 
GGATTTGAGA TGAAGCTGGA TGCTATGTCT TTGCTGGCCG CCAAAGCTTC TGGAGAGCTT 
GCTAGCAATA TTAAATATAA AGAAGAGTAT GAAAAAACGA AAGGCAAAGT CATGGGCACT 
ACAGACTCGA GGCTTCTGCA CTCTCTGCAG GTGGCCAAGA TGAGCAGTGA GGTTGAATAC 
AAGAAAGGCT TTGAAGAGAG TAAGACCCAC TTTCACCTAC CCATGGATAT GGTGCACATC 
AAGCATGCTA AGAAGGCCCA AGCTCTGGCC AGTGACCTGG ACTACAGGAA AAAGCTGCAT 
GAATACACTG TACTGCCTGA AGATATGAAG ACTCAGTGGG CCAAGAAAGC CTATGGGCTC 
CAGAGTGAGC TGCAGTACAA GGCCGACCTA GCATGGATGA AAGGAGTCGG GTGGCTGACG 
GAGGGGAGTC TCAATTTGGA ACAGGCCAAG AAGGCTGGCC AGCTGGCCAG CGAGAAAAAC 
TACAGGCAGA GGGTGGATGA GCTGAAGTTC ACCAGTGTGA CCGACAGCTC CCAGATGGAG 
CACGCCAAGA AGAGCCAGGA GCTGCAGAGC GGGGTGGCCT ACAAGGCAGG AAATGAGCAG 
TCTGTCCATC AGTACAGCAT CAGCAAAGAT GAACCTCTCT TCTTACAGGC CCGAGTCAAT 
GCCGCCAATC TCAGTGAGAA ACTGTATAAG AGCAGCTGGG AAAACCAGAA GGCAAAAGGT 
TTTGAGCTGC GTCTGGACTC CTTGGGCTTC CTGGCAGCCA AAGCCAACAG GGACCTGGCG 
AGCGAGGTGA AGTACAAGGA AGATTACGAG AGGTCCCGAG GTAAGCTCAT TGGAGCGAAG 
GATGCCCAGG GGGATTCACA AATGAGCCAC TCGCTGCAAA TGTCCAAGCT GCAGAGTGAA 
CTCGAGTACA AGAAAGGGTT CGAGGACACC AGATCCCAGT GCCACGTCTC CCTGGACATG 
ATCCACCTTG TGCATGCCCG CAAGGCTCAG CATTTAGCCA CAGACATAGG CTACAAGACG 
GCATCTCATC AGTTCACCGC TTTGCCCACC GACATGAAGG TGGAATGGGC CAAGAAGGCA 
TACGGTTTAC AGAGTGATAA CCAATACAGG GCAGATGTGA AGTGGATGAA AGGCACGGGC 
TGGGTCGCCA CCGGGTCATT AAATGTGGAG CAGGCGAAGA AGGCAGGAGA ACTCATTAGC 
GAGAAGAAGT ACCGTCAGCA TCCAGATGCT TTGAAGTTTA CCAGTATTAA AGACACTCCG 
GAGATGGTCC AGGCCAGAAT TAGTTATACC CAAGCAGTGG ATAGGTTATA CAGGGAACAA 
GGAGAAAATA TAAAGCATCA TTACACTCAG ACAGCCGACC TCCCTGAAGT CCTGCTGGCC 
AAGCTGAATG CTATGAATAT CAGTGAGACG CGTTATAAGG AATCCTGGAG CAAACTTCGG 
GACGGTGGCT ATAAACTGAG GTTGGATGCC ATTCCGTTCC AGGCAGCAAA GGCTTCTGGC 
GAAATCATAA GTGATTACAA ATACAAAGAG GCATTTGAGA AAATGAAAGG GCAGATGCTT 
GGCTCCCGGA GCTTGGAAGA CGATATCAGC CTTGCACATT CAGTGTATGC GAACTCGCTG 
CAGAGCGAAG TGAATTACAA GAAAGGCTTT GAGCTCTCAA AGGCACACTT CCATCTGCCG 
TTGGACATGG TAACCCTGGT GCATGCCAAG AAGGCTCAGA GCCTGGCCAG CGACCAGGAC 
TACAGACACC CGCTCCCCCA GTACACATCC TTGGCTGAAG ACCTGAGGCT CAGCTGCGCC 
AAGAAAGCTC ACAAGCTGCA GAGTGAGAAT TTGTACCGCT CAGACCTGAA CTTTATGCGA 
GGCGTTCCCT GTGTCATTCC GGGCACATTA GAGATTGAAG GGAGAAAGAA AGCATCTGAA 
CTCATCAGTG AGAGTAAATA CCGTCAGCAT CCCCAGTCAT TCAAGTACAC AGCTGTGACA 
GACACTCCCA GCCTCCTTCA TGCAAAACTC AGCAACCAGA TTACCAATGA GCGCCTCTAT 
AAAGCAGCCG GAGAGGATGA GAGGCACCGG TACACAATGA CCCTGGGTCT GCCCGAGTTT 
GTCCGAGCGA AAGCGAACGC AGCCAATCTG AGTGACGCAA AATACAAGGA GTCTTGGCGT 
AATCTCCGTG CTCAAGGCTA CAAGCTGACA ATAGACGCTC TCCCCTTCCA GGCTGCTCGC 
GTCTCTGGAG ATATAGCCAG TGATTTTCTC TACAGACATG ACTTTGTGAA GGAGCGAGGC 
AGGCTGATCG GGGCCCGGAG TGTGAGTGAT GACCCCCGAC TCCAGCACTG CTGGCGGGTA 
GGCCAGTTGC AGAGCGAGCT TCAGTACAGG CGGGGAGCGG CGAGCAGCCA AGCCCACTAC 
CACCTGCCCA TGGACATGGT GCACCTGGTC CATGCCAGGA AGGCCCAGGC CCTAGCCAGC 
GACCACGACT ACAGGACACG GTGCCATGAG TTCACAGCTC TGCCTGAGGA CCTGAAGATG 
TCCTGGGCCA AGAGAGCTCA CGCCCTGCAG AGTGAGTTTC GCTACAAGTC AGACCTGATG 
GGCATGAAGG GGACAGGATG GCTGACGCTG AGTTCGCCGC AGCTAGAGAG TGCAAAGAAG 
GCCGGAGAAC TCATCAGCGA GACCAAATAC CGTAAGAAAC CAGATAGTAT CAAGTTCACC 
ACGGTGGTTG ACTCCCCAGA CCTGGTTCAC GCCAAGAACA GCTACATGCA TTGCAATGAG 
CGCCTGTACA GATTGGGGGA TGCGGAATCC CTGCACAGAT ATACGCTGAT CCCCGACCAC 
CCCGATTTCA CCAGAGCCCG CCTCAACGCG CTGCATCTGA GTGATAAAGT CTACAGAAAC 
TCTTGGGAAC AGACCCGGGC TGGTGGCTAT GACTTCCGGC TGGATGCCAT CCCATTCCAG 
ACTGCCCGGG CGTCCCGGGA GATCGCCAGT GATTTCCGGT ACAAAGAGGC CTTCCTGCGG 
GACCGGGGCC TACAGATTGG TTACCGCAGC ATCCACGACG ACCCCAGGAT GAGGCATTTC 
CTCAGCGTCG GCAAACTCCA GAGTGACAAC GAGTACAAGA AGGACTTTGC CAAGAGTCGG 
TCCCAGTTCC ATAGCCGCCC GGACCAGCCC GGCTTCCTCC AGGCCAGGAG GAGCCAGCAG 
CTGGCCAGCG ACGTGTGCTA CCGGCAGCCC CTGCCCCAGC CCACATGCGA CCCGGAGCAG 
CTAGGCCTGA AGCACGCACA GAAGGCCCAC CAGCTGCAGA GTGACGTCAA GTACAAATCA 
GACTTGAACC TGACCAGAGG CGTTGGCTGG ACACCCCCCG GCTCCTACAA AGTGGAAATG 
GCTCGGCGGG CGGCAGAACT GGCCAAGGCG AGGGGCCTGG GTCTCCGGGG CGCTTGGGGG 
GGGCCAGAGG CTGTGGAGCC CGCAGAGAAT CAGAGGGGGG ACGTGAACCC AGACGCCACC 
GAGATTCTGC ACGTCAAAAG GAGGAAGGCT CTGCCGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_864817.1
CDS	202..5283
Translation	MNVQACSRCGYGVYPAEKISCLDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRKSPETIHGIDEQDEGERFKSVFHWDMKSRDGTAAPNRQPLMNERAYWNGYGEGNAWCPGAMPDPEIVRMVETRKSLGEEYTEDYEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKRGHDERISRFTTVADTPELLRAQAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMKGMASPAAGAAGTRECMDRYGQGYLEEYQEHRGKGSFPAMITPAYQNAKKANELASDIKYRQDFNKMKGPAHYHSLPAQDNLVLKRAQSVNKLVSENKYKENYQNHMRGHYEGVGMDRRTLHAMKVGSLASNIAYKADYKHDVVDYNYPATLTPSYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIIQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAELFSEVKYKEGWEKAKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKVMGTTDSRLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVHIKHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLASEKNYRQRVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYSISKDEPLFLQARVNAANLSEKLYKSSWENQKAKGFELRLDSLGFLAAKANRDLASEVKYKEDYERSRGKLIGAKDAQGDSQMSHSLQMSKLQSELEYKKGFEDTRSQCHVSLDMIHLVHARKAQHLATDIGYKTASHQFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTADLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASGEIISDYKYKEAFEKMKGQMLGSRSLEDDISLAHSVYANSLQSEVNYKKGFELSKAHFHLPLDMVTLVHAKKAQSLASDQDYRHPLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVPCVIPGTLEIEGRKKASELISESKYRQHPQSFKYTAVTDTPSLLHAKLSNQITNERLYKAAGEDERHRYTMTLGLPEFVRAKANAANLSDAKYKESWRNLRAQGYKLTIDALPFQAARVSGDIASDFLYRHDFVKERGRLIGARSVSDDPRLQHCWRVGQLQSELQYRRGAASSQAHYHLPMDMVHLVHARKAQALASDHDYRTRCHEFTALPEDLKMSWAKRAHALQSEFRYKSDLMGMKGTGWLTLSSPQLESAKKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERLYRLGDAESLHRYTLIPDHPDFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGYRSIHDDPRMRHFLSVGKLQSDNEYKKDFAKSRSQFHSRPDQPGFLQARRSQQLASDVCYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELAKARGLGLRGAWGGPEAVEPAENQRGDVNPDATEILHVKRRKALPL

Target ORF information:

RefSeq Version	XM_859724.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_859724.4

ORF Insert Sequence:

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGGAGTA TACAGAAGAC 
TATGAACAAC AGAGGGGCAA AGGGAGCTTT CCAGCCATGA TCACACCTGC CTATCAAAGA 
GCCAAGAAAG CTAACCAGCT GGCCAGCCAA GTGGAATACA AGAGAGGGCA TGACGAGCGC 
ATCTCCAGGT TTACCACGGT GGCGGATACC CCTGAGCTGC TACGGGCCCA GGCAGGGGGA 
CAGCTTCAAA GTGATGTGAG ATACACAGAA GACTATGAAC AACAGAGAGG AAAAGGCAGT 
TTCCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAGA GAGCCAATGA GCTGGCTAGC 
GATGTGAGGT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTAG TCCAGCTGCT 
GGAGCTGCAG GCACAAGGGA ATGCATGGAC CGATATGGCC AGGGTTACTT GGAGGAATAT 
CAGGAGCACA GAGGAAAAGG CAGCTTCCCC GCCATGATCA CCCCAGCTTA TCAGAATGCC 
AAGAAAGCCA ACGAACTCGC TAGTGACATA AAATACAGGC AGGACTTCAA TAAGATGAAA 
GGCCCTGCAC ATTATCACTC CCTCCCAGCC CAGGACAACT TGGTTCTCAA GCGGGCCCAG 
AGCGTGAACA AGCTTGTGAG CGAGAATAAA TATAAAGAAA ATTACCAGAA CCACATGAGA 
GGCCACTATG AAGGAGTTGG TATGGACAGA CGGACTCTCC ATGCTATGAA GGTTGGAAGC 
TTGGCAAGCA ATATTGCCTA CAAGGCTGAT TATAAACACG ATGTTGTGGA CTACAACTAC 
CCAGCCACTC TTACTCCCTC TTACCAGACA ACAATGAAAC TTGTCCCCTT GAAAGATGTC 
AACTACAGGC AAAGTATCGA CAAGTTGAAG TACAGCTCTG TGACCAACAC CCCTCAGATT 
ATCCAAGCCA AAATCAATGC CCAACAACTG AGTCATGTGA ATTATCGTGC TGACTATGAG 
AAAAATAAGT TGAACTACAC CTTGCCACAG GATGTACCTC AGCTGGTGAA GGCCAAAACC 
AATGCTGAAC TATTTAGTGA GGTTAAGTAT AAAGAAGGCT GGGAGAAGGC CAAGGGGAAA 
GGATTTGAGA TGAAGCTGGA TGCTATGTCT TTGCTGGCCG CCAAAGCTTC TGGAGAGCTT 
GCTAGCAATA TTAAATATAA AGAAGAGTAT GAAAAAACGA AAGGCAAAGT CATGGGCACT 
ACAGACTCGA GGCTTCTGCA CTCTCTGCAG GTGGCCAAGA TGAGCAGTGA GGTTGAATAC 
AAGAAAGGCT TTGAAGAGAG TAAGACCCAC TTTCACCTAC CCATGGATAT GGTGCACATC 
AAGCATGCTA AGAAGGCCCA AGCTCTGGCC AGTGACCTGG ACTACAGGAA AAAGCTGCAT 
GAATACACTG TACTGCCTGA AGATATGAAG ACTCAGTGGG CCAAGAAAGC CTATGGGCTC 
CAGAGTGAGC TGCAGTACAA GGCCGACCTA GCATGGATGA AAGGAGTCGG GTGGCTGACG 
GAGGGGAGTC TCAATTTGGA ACAGGCCAAG AAGGCTGGCC AGCTGGCCAG CGAGAAAAAC 
TACAGGCAGA GGGTGGATGA GCTGAAGTTC ACCAGTGTGA CCGACAGCTC CCAGATGGAG 
CACGCCAAGA AGAGCCAGGA GCTGCAGAGC GGGGTGGCCT ACAAGGCAGG AAATGAGCAG 
TCTGTCCATC AGTACAGCAT CAGCAAAGAT GAACCTCTCT TCTTACAGGC CCGAGTCAAT 
GCCGCCAATC TCAGTGAGAA ACTGTATAAG AGCAGCTGGG AAAACCAGAA GGCAAAAGGT 
TTTGAGCTGC GTCTGGACTC CTTGGGCTTC CTGGCAGCCA AAGCCAACAG GGACCTGGCG 
AGCGAGGTGA AGTACAAGGA AGATTACGAG AGGTCCCGAG GTAAGCTCAT TGGAGCGAAG 
GATGCCCAGG GGGATTCACA AATGAGCCAC TCGCTGCAAA TGTCCAAGCT GCAGAGTGAA 
CTCGAGTACA AGAAAGGGTT CGAGGACACC AGATCCCAGT GCCACGTCTC CCTGGACATG 
ATCCACCTTG TGCATGCCCG CAAGGCTCAG CATTTAGCCA CAGACATAGG CTACAAGACG 
GCATCTCATC AGTTCACCGC TTTGCCCACC GACATGAAGG TGGAATGGGC CAAGAAGGCA 
TACGGTTTAC AGAGTGATAA CCAATACAGG GCAGATGTGA AGTGGATGAA AGGCACGGGC 
TGGGTCGCCA CCGGGTCATT AAATGTGGAG CAGGCGAAGA AGGCAGGAGA ACTCATTAGC 
GAGAAGAAGT ACCGTCAGCA TCCAGATGCT TTGAAGTTTA CCAGTATTAA AGACACTCCG 
GAGATGGTCC AGGCCAGAAT TAGTTATACC CAAGCAGTGG ATAGGTTATA CAGGGAACAA 
GGAGAAAATA TAAAGCATCA TTACACTCAG ACAGCCGACC TCCCTGAAGT CCTGCTGGCC 
AAGCTGAATG CTATGAATAT CAGTGAGACG CGTTATAAGG AATCCTGGAG CAAACTTCGG 
GACGGTGGCT ATAAACTGAG GTTGGATGCC ATTCCGTTCC AGGCAGCAAA GGCTTCTGGC 
GAAATCATAA GTGATTACAA ATACAAAGAG GCATTTGAGA AAATGAAAGG GCAGATGCTT 
GGCTCCCGGA GCTTGGAAGA CGATATCAGC CTTGCACATT CAGTGTATGC GAACTCGCTG 
CAGAGCGAAG TGAATTACAA GAAAGGCTTT GAGCTCTCAA AGGCACACTT CCATCTGCCG 
TTGGACATGG TAACCCTGGT GCATGCCAAG AAGGCTCAGA GCCTGGCCAG CGACCAGGAC 
TACAGACACC CGCTCCCCCA GTACACATCC TTGGCTGAAG ACCTGAGGCT CAGCTGCGCC 
AAGAAAGCTC ACAAGCTGCA GAGTGAGAAT TTGTACCGCT CAGACCTGAA CTTTATGCGA 
GGCGTTCCCT GTGTCATTCC GGGCACATTA GAGATTGAAG GGAGAAAGAA AGCATCTGAA 
CTCATCAGTG AGAGTAAATA CCGTCAGCAT CCCCAGTCAT TCAAGTACAC AGCTGTGACA 
GACACTCCCA GCCTCCTTCA TGCAAAACTC AGCAACCAGA TTACCAATGA GCGCCTCTAT 
AAAGCAGCCG GAGAGGATGA GAGGCACCGG TACACAATGA CCCTGGGTCT GCCCGAGTTT 
GTCCGAGCGA AAGCGAACGC AGCCAATCTG AGTGACGCAA AATACAAGGA GTCTTGGCGT 
AATCTCCGTG CTCAAGGCTA CAAGCTGACA ATAGACGCTC TCCCCTTCCA GGCTGCTCGC 
GTCTCTGGAG ATATAGCCAG TGATTTTCTC TACAGACATG ACTTTGTGAA GGAGCGAGGC 
AGGCTGATCG GGGCCCGGAG TGTGAGTGAT GACCCCCGAC TCCAGCACTG CTGGCGGGTA 
GGCCAGTTGC AGAGCGAGCT TCAGTACAGG CGGGGAGCGG CGAGCAGCCA AGCCCACTAC 
CACCTGCCCA TGGACATGGT GCACCTGGTC CATGCCAGGA AGGCCCAGGC CCTAGCCAGC 
GACCACGACT ACAGGACACG GTGCCATGAG TTCACAGCTC TGCCTGAGGA CCTGAAGATG 
TCCTGGGCCA AGAGAGCTCA CGCCCTGCAG AGTGAGTTTC GCTACAAGTC AGACCTGATG 
GGCATGAAGG GGACAGGATG GCTGACGCTG AGTTCGCCGC AGCTAGAGAG TGCAAAGAAG 
GCCGGAGAAC TCATCAGCGA GACCAAATAC CGTAAGAAAC CAGATAGTAT CAAGTTCACC 
ACGGTGGTTG ACTCCCCAGA CCTGGTTCAC GCCAAGAACA GCTACATGCA TTGCAATGAG 
CGCCTGTACA GATTGGGGGA TGCGGAATCC CTGCACAGAT ATACGCTGAT CCCCGACCAC 
CCCGATTTCA CCAGAGCCCG CCTCAACGCG CTGCATCTGA GTGATAAAGT CTACAGAAAC 
TCTTGGGAAC AGACCCGGGC TGGTGGCTAT GACTTCCGGC TGGATGCCAT CCCATTCCAG 
ACTGCCCGGG CGTCCCGGGA GATCGCCAGT GATTTCCGGT ACAAAGAGGC CTTCCTGCGG 
GACCGGGGCC TACAGATTGG TTACCGCAGC ATCCACGACG ACCCCAGGAT GAGGCATTTC 
CTCAGCGTCG GCAAACTCCA GAGTGACAAC GAGTACAAGA AGGACTTTGC CAAGAGTCGG 
TCCCAGTTCC ATAGCCGCCC GGACCAGCCC GGCTTCCTCC AGGCCAGGAG GAGCCAGCAG 
CTGGCCAGCG ACGTGTGCTA CCGGCAGCCC CTGCCCCAGC CCACATGCGA CCCGGAGCAG 
CTAGGCCTGA AGCACGCACA GAAGGCCCAC CAGCTGCAGA GTGACGTCAA GTACAAATCA 
GACTTGAACC TGACCAGAGG CGTTGGCTGG ACACCCCCCG GCTCCTACAA AGTGGAAATG 
GCTCGGCGGG CGGCAGAACT GGCCAAGGCG AGGGGCCTGG GTCTCCGGGG CGCTTGGGGG 
GGGCCAGAGG CTGTGGAGCC CGCAGAGAAT CAGAGGGGGG ACGTGAACCC AGACGCCACC 
GAGATTCTGC ACGTCAAAAG GAGGAAGGCT CTGCCGTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_014108945.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964420.1 Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X1, mRNA.

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CloneID	OCe43944
Clone ID Related Accession (Same CDS sequence)	XM_014108945.1 , XM_014108945.2
Accession Version	XM_014108945.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	nebulin-related-anchoring protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGCAGGA GTATACAGAA 
GACTATGAAC AACAGAGGGG CAAAGGGAGC TTTCCAGCCA TGATCACACC TGCCTATCAA 
AGAGCCAAGA AAGCTAACCA GCTGGCCAGC CAAGTGGAAT ACAAGAGAGG GCATGACGAG 
CGCATCTCCA GGTTTACCAC GGTGGCGGAT ACCCCTGAGC TGCTACGGGC CCAGGCAGGG 
GGACAGCTTC AAAGTGATGT GAGATACACA GAAGACTATG AACAACAGAG AGGAAAAGGC 
AGTTTCCCTG CTATGATCAC GCCTGCTTAT CAGATAGCCA AGAGAGCCAA TGAGCTGGCT 
AGCGATGTGA GGTACCATCA ACAATATCAA AGAGAAATGA AGGGAATGGC TAGTCCAGCT 
GCTGGAGCTG CAGGCACAAG GGAATGCATG GACCGATATG GCCAGCAGGG TTACTTGGAG 
GAATATCAGG AGCACAGAGG AAAAGGCAGC TTCCCCGCCA TGATCACCCC AGCTTATCAG 
AATGCCAAGA AAGCCAACGA ACTCGCTAGT GACATAAAAT ACAGGCAGGA CTTCAATAAG 
ATGAAAGGCC CTGCACATTA TCACTCCCTC CCAGCCCAGG ACAACTTGGT TCTCAAGCGG 
GCCCAGAGCG TGAACAAGCT TGTGAGCGAG GTGGAATATA AGAAGGACCT GGAAAGTAGT 
AAAGGTCACA GTATCAACTA CTGTGAGACG CCTCAATTCA GGAACGTGAG CAAGATCTCA 
AAATTCACCA GTGATAATAA ATATAAAGAA AATTACCAGA ACCACATGAG AGGCCACTAT 
GAAGGAGTTG GTATGGACAG ACGGACTCTC CATGCTATGA AGGTTGGAAG CTTGGCAAGC 
AATATTGCCT ACAAGGCTGA TTATAAACAC GATGTTGTGG ACTACAACTA CCCAGCCACT 
CTTACTCCCT CTTACCAGAC AACAATGAAA CTTGTCCCCT TGAAAGATGT CAACTACAGG 
CAAAGTATCG ACAAGTTGAA GTACAGCTCT GTGACCAACA CCCCTCAGAT TATCCAAGCC 
AAAATCAATG CCCAACAACT GAGTCATGTG AATTATCGTG CTGACTATGA GAAAAATAAG 
TTGAACTACA CCTTGCCACA GGATGTACCT CAGCTGGTGA AGGCCAAAAC CAATGCTGAA 
CTATTTAGTG AGGTTAAGTA TAAAGAAGGC TGGGAGAAGG CCAAGGGGAA AGGATTTGAG 
ATGAAGCTGG ATGCTATGTC TTTGCTGGCC GCCAAAGCTT CTGGAGAGCT TGCTAGCAAT 
ATTAAATATA AAGAAGAGTA TGAAAAAACG AAAGGCAAAG TCATGGGCAC TACAGACTCG 
AGGCTTCTGC ACTCTCTGCA GGTGGCCAAG ATGAGCAGTG AGGTTGAATA CAAGAAAGGC 
TTTGAAGAGA GTAAGACCCA CTTTCACCTA CCCATGGATA TGGTGCACAT CAAGCATGCT 
AAGAAGGCCC AAGCTCTGGC CAGTGACCTG GACTACAGGA AAAAGCTGCA TGAATACACT 
GTACTGCCTG AAGATATGAA GACTCAGTGG GCCAAGAAAG CCTATGGGCT CCAGAGTGAG 
CTGCAGTACA AGGCCGACCT AGCATGGATG AAAGGAGTCG GGTGGCTGAC GGAGGGGAGT 
CTCAATTTGG AACAGGCCAA GAAGGCTGGC CAGCTGGCCA GCGAGAAAAA CTACAGGCAG 
AGGGTGGATG AGCTGAAGTT CACCAGTGTG ACCGACAGCT CCCAGATGGA GCACGCCAAG 
AAGAGCCAGG AGCTGCAGAG CGGGGTGGCC TACAAGGCAG GAAATGAGCA GTCTGTCCAT 
CAGTACAGCA TCAGCAAAGA TGAACCTCTC TTCTTACAGG CCCGAGTCAA TGCCGCCAAT 
CTCAGTGAGA AACTGTATAA GAGCAGCTGG GAAAACCAGA AGGCAAAAGG TTTTGAGCTG 
CGTCTGGACT CCTTGGGCTT CCTGGCAGCC AAAGCCAACA GGGACCTGGC GAGCGAGGTG 
AAGTACAAGG AAGATTACGA GAGGTCCCGA GGTAAGCTCA TTGGAGCGAA GGATGCCCAG 
GGGGATTCAC AAATGAGCCA CTCGCTGCAA ATGTCCAAGC TGCAGAGTGA ACTCGAGTAC 
AAGAAAGGGT TCGAGGACAC CAGATCCCAG TGCCACGTCT CCCTGGACAT GATCCACCTT 
GTGCATGCCC GCAAGGCTCA GCATTTAGCC ACAGACATAG GCTACAAGAC GGCATCTCAT 
CAGTTCACCG CTTTGCCCAC CGACATGAAG GTGGAATGGG CCAAGAAGGC ATACGGTTTA 
CAGAGTGATA ACCAATACAG GGCAGATGTG AAGTGGATGA AAGGCACGGG CTGGGTCGCC 
ACCGGGTCAT TAAATGTGGA GCAGGCGAAG AAGGCAGGAG AACTCATTAG CGAGAAGAAG 
TACCGTCAGC ATCCAGATGC TTTGAAGTTT ACCAGTATTA AAGACACTCC GGAGATGGTC 
CAGGCCAGAA TTAGTTATAC CCAAGCAGTG GATAGGTTAT ACAGGGAACA AGGAGAAAAT 
ATAAAGCATC ATTACACTCA GACAGCCGAC CTCCCTGAAG TCCTGCTGGC CAAGCTGAAT 
GCTATGAATA TCAGTGAGAC GCGTTATAAG GAATCCTGGA GCAAACTTCG GGACGGTGGC 
TATAAACTGA GGTTGGATGC CATTCCGTTC CAGGCAGCAA AGGCTTCTGG CGAAATCATA 
AGTGATTACA AATACAAAGA GGCATTTGAG AAAATGAAAG GGCAGATGCT TGGCTCCCGG 
AGCTTGGAAG ACGATATCAG CCTTGCACAT TCAGTGTATG CGAACTCGCT GCAGAGCGAA 
GTGAATTACA AGAAAGGCTT TGAGCTCTCA AAGGCACACT TCCATCTGCC GTTGGACATG 
GTAACCCTGG TGCATGCCAA GAAGGCTCAG AGCCTGGCCA GCGACCAGGA CTACAGACAC 
CCGCTCCCCC AGTACACATC CTTGGCTGAA GACCTGAGGC TCAGCTGCGC CAAGAAAGCT 
CACAAGCTGC AGAGTGAGAA TTTGTACCGC TCAGACCTGA ACTTTATGCG AGGCGTTCCC 
TGTGTCATTC CGGGCACATT AGAGATTGAA GGGAGAAAGA AAGCATCTGA ACTCATCAGT 
GAGAGTAAAT ACCGTCAGCA TCCCCAGTCA TTCAAGTACA CAGCTGTGAC AGACACTCCC 
AGCCTCCTTC ATGCAAAACT CAGCAACCAG ATTACCAATG AGCGCCTCTA TAAAGCAGCC 
GGAGAGGATG AGAGGCACCG GTACACAATG ACCCTGGGTC TGCCCGAGTT TGTCCGAGCG 
AAAGCGAACG CAGCCAATCT GAGTGACGCA AAATACAAGG AGTCTTGGCG TAATCTCCGT 
GCTCAAGGCT ACAAGCTGAC AATAGACGCT CTCCCCTTCC AGGCTGCTCG CGTCTCTGGA 
GATATAGCCA GTGATTTTCT CTACAGACAT GACTTTGTGA AGGAGCGAGG CAGGCTGATC 
GGGGCCCGGA GTGTGAGTGA TGACCCCCGA CTCCAGCACT GCTGGCGGGT AGGCCAGTTG 
CAGAGCGAGC TTCAGTACAG GCGGGGAGCG GCGAGCAGCC AAGCCCACTA CCACCTGCCC 
ATGGACATGG TGCACCTGGT CCATGCCAGG AAGGCCCAGG CCCTAGCCAG CGACCACGAC 
TACAGGACAC GGTGCCATGA GTTCACAGCT CTGCCTGAGG ACCTGAAGAT GTCCTGGGCC 
AAGAGAGCTC ACGCCCTGCA GAGTGAGTTT CGCTACAAGT CAGACCTGAT GGGCATGAAG 
GGGACAGGAT GGCTGACGCT GAGTTCGCCG CAGCTAGAGA GTGCAAAGAA GGCCGGAGAA 
CTCATCAGCG AGACCAAATA CCGTAAGAAA CCAGATAGTA TCAAGTTCAC CACGGTGGTT 
GACTCCCCAG ACCTGGTTCA CGCCAAGAAC AGCTACATGC ATTGCAATGA GCGCCTGTAC 
AGATTGGGGG ATGCGGAATC CCTGCACAGA TATACGCTGA TCCCCGACCA CCCCGATTTC 
ACCAGAGCCC GCCTCAACGC GCTGCATCTG AGTGATAAAG TCTACAGAAA CTCTTGGGAA 
CAGACCCGGG CTGGTGGCTA TGACTTCCGG CTGGATGCCA TCCCATTCCA GACTGCCCGG 
GCGTCCCGGG AGATCGCCAG TGATTTCCGG TACAAAGAGG CCTTCCTGCG GGACCGGGGC 
CTACAGATTG GTTACCGCAG CATCCACGAC GACCCCAGGA TGAGGCATTT CCTCAGCGTC 
GGCAAACTCC AGAGTGACAA CGAGTACAAG AAGGACTTTG CCAAGAGTCG GTCCCAGTTC 
CATAGCCGCC CGGACCAGCC CGGCTTCCTC CAGGCCAGGA GGAGCCAGCA GCTGGCCAGC 
GACGTGTGCT ACCGGCAGCC CCTGCCCCAG CCCACATGCG ACCCGGAGCA GCTAGGCCTG 
AAGCACGCAC AGAAGGCCCA CCAGCTGCAG AGTGACGTCA AGTACAAATC AGACTTGAAC 
CTGACCAGAG GCGTTGGCTG GACACCCCCC GGCTCCTACA AAGTGGAAAT GGCTCGGCGG 
GCGGCAGAAC TGGCCAAGGC GAGGGGCCTG GGTCTCCGGG GCGCTTGGGG GGGGCCAGAG 
GCTGTGGAGC CCGCAGAGAA TCAGAGGGGG GACGTGAACC CAGACGCCAC CGAGATTCTG 
CACGTCAAAA GGAGGAAGGC TCTGCCGTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964420.1
CDS	203..5395
Translation	MNVQACSRCGYGVYPAEKISCLDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRKSPETIHGIDEQDEGERFKSVFHWDMKSRDGTAAPNRQPLMNERAYWNGYGEGNAWCPGAMPDPEIVRMVETRKSLGEQEYTEDYEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKRGHDERISRFTTVADTPELLRAQAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMKGMASPAAGAAGTRECMDRYGQQGYLEEYQEHRGKGSFPAMITPAYQNAKKANELASDIKYRQDFNKMKGPAHYHSLPAQDNLVLKRAQSVNKLVSEVEYKKDLESSKGHSINYCETPQFRNVSKISKFTSDNKYKENYQNHMRGHYEGVGMDRRTLHAMKVGSLASNIAYKADYKHDVVDYNYPATLTPSYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIIQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAELFSEVKYKEGWEKAKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKVMGTTDSRLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVHIKHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLASEKNYRQRVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYSISKDEPLFLQARVNAANLSEKLYKSSWENQKAKGFELRLDSLGFLAAKANRDLASEVKYKEDYERSRGKLIGAKDAQGDSQMSHSLQMSKLQSELEYKKGFEDTRSQCHVSLDMIHLVHARKAQHLATDIGYKTASHQFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTADLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASGEIISDYKYKEAFEKMKGQMLGSRSLEDDISLAHSVYANSLQSEVNYKKGFELSKAHFHLPLDMVTLVHAKKAQSLASDQDYRHPLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVPCVIPGTLEIEGRKKASELISESKYRQHPQSFKYTAVTDTPSLLHAKLSNQITNERLYKAAGEDERHRYTMTLGLPEFVRAKANAANLSDAKYKESWRNLRAQGYKLTIDALPFQAARVSGDIASDFLYRHDFVKERGRLIGARSVSDDPRLQHCWRVGQLQSELQYRRGAASSQAHYHLPMDMVHLVHARKAQALASDHDYRTRCHEFTALPEDLKMSWAKRAHALQSEFRYKSDLMGMKGTGWLTLSSPQLESAKKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERLYRLGDAESLHRYTLIPDHPDFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGYRSIHDDPRMRHFLSVGKLQSDNEYKKDFAKSRSQFHSRPDQPGFLQARRSQQLASDVCYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELAKARGLGLRGAWGGPEAVEPAENQRGDVNPDATEILHVKRRKALPL

Target ORF information:

RefSeq Version	XM_014108945.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108945.1

ORF Insert Sequence:

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGCAGGA GTATACAGAA 
GACTATGAAC AACAGAGGGG CAAAGGGAGC TTTCCAGCCA TGATCACACC TGCCTATCAA 
AGAGCCAAGA AAGCTAACCA GCTGGCCAGC CAAGTGGAAT ACAAGAGAGG GCATGACGAG 
CGCATCTCCA GGTTTACCAC GGTGGCGGAT ACCCCTGAGC TGCTACGGGC CCAGGCAGGG 
GGACAGCTTC AAAGTGATGT GAGATACACA GAAGACTATG AACAACAGAG AGGAAAAGGC 
AGTTTCCCTG CTATGATCAC GCCTGCTTAT CAGATAGCCA AGAGAGCCAA TGAGCTGGCT 
AGCGATGTGA GGTACCATCA ACAATATCAA AGAGAAATGA AGGGAATGGC TAGTCCAGCT 
GCTGGAGCTG CAGGCACAAG GGAATGCATG GACCGATATG GCCAGCAGGG TTACTTGGAG 
GAATATCAGG AGCACAGAGG AAAAGGCAGC TTCCCCGCCA TGATCACCCC AGCTTATCAG 
AATGCCAAGA AAGCCAACGA ACTCGCTAGT GACATAAAAT ACAGGCAGGA CTTCAATAAG 
ATGAAAGGCC CTGCACATTA TCACTCCCTC CCAGCCCAGG ACAACTTGGT TCTCAAGCGG 
GCCCAGAGCG TGAACAAGCT TGTGAGCGAG GTGGAATATA AGAAGGACCT GGAAAGTAGT 
AAAGGTCACA GTATCAACTA CTGTGAGACG CCTCAATTCA GGAACGTGAG CAAGATCTCA 
AAATTCACCA GTGATAATAA ATATAAAGAA AATTACCAGA ACCACATGAG AGGCCACTAT 
GAAGGAGTTG GTATGGACAG ACGGACTCTC CATGCTATGA AGGTTGGAAG CTTGGCAAGC 
AATATTGCCT ACAAGGCTGA TTATAAACAC GATGTTGTGG ACTACAACTA CCCAGCCACT 
CTTACTCCCT CTTACCAGAC AACAATGAAA CTTGTCCCCT TGAAAGATGT CAACTACAGG 
CAAAGTATCG ACAAGTTGAA GTACAGCTCT GTGACCAACA CCCCTCAGAT TATCCAAGCC 
AAAATCAATG CCCAACAACT GAGTCATGTG AATTATCGTG CTGACTATGA GAAAAATAAG 
TTGAACTACA CCTTGCCACA GGATGTACCT CAGCTGGTGA AGGCCAAAAC CAATGCTGAA 
CTATTTAGTG AGGTTAAGTA TAAAGAAGGC TGGGAGAAGG CCAAGGGGAA AGGATTTGAG 
ATGAAGCTGG ATGCTATGTC TTTGCTGGCC GCCAAAGCTT CTGGAGAGCT TGCTAGCAAT 
ATTAAATATA AAGAAGAGTA TGAAAAAACG AAAGGCAAAG TCATGGGCAC TACAGACTCG 
AGGCTTCTGC ACTCTCTGCA GGTGGCCAAG ATGAGCAGTG AGGTTGAATA CAAGAAAGGC 
TTTGAAGAGA GTAAGACCCA CTTTCACCTA CCCATGGATA TGGTGCACAT CAAGCATGCT 
AAGAAGGCCC AAGCTCTGGC CAGTGACCTG GACTACAGGA AAAAGCTGCA TGAATACACT 
GTACTGCCTG AAGATATGAA GACTCAGTGG GCCAAGAAAG CCTATGGGCT CCAGAGTGAG 
CTGCAGTACA AGGCCGACCT AGCATGGATG AAAGGAGTCG GGTGGCTGAC GGAGGGGAGT 
CTCAATTTGG AACAGGCCAA GAAGGCTGGC CAGCTGGCCA GCGAGAAAAA CTACAGGCAG 
AGGGTGGATG AGCTGAAGTT CACCAGTGTG ACCGACAGCT CCCAGATGGA GCACGCCAAG 
AAGAGCCAGG AGCTGCAGAG CGGGGTGGCC TACAAGGCAG GAAATGAGCA GTCTGTCCAT 
CAGTACAGCA TCAGCAAAGA TGAACCTCTC TTCTTACAGG CCCGAGTCAA TGCCGCCAAT 
CTCAGTGAGA AACTGTATAA GAGCAGCTGG GAAAACCAGA AGGCAAAAGG TTTTGAGCTG 
CGTCTGGACT CCTTGGGCTT CCTGGCAGCC AAAGCCAACA GGGACCTGGC GAGCGAGGTG 
AAGTACAAGG AAGATTACGA GAGGTCCCGA GGTAAGCTCA TTGGAGCGAA GGATGCCCAG 
GGGGATTCAC AAATGAGCCA CTCGCTGCAA ATGTCCAAGC TGCAGAGTGA ACTCGAGTAC 
AAGAAAGGGT TCGAGGACAC CAGATCCCAG TGCCACGTCT CCCTGGACAT GATCCACCTT 
GTGCATGCCC GCAAGGCTCA GCATTTAGCC ACAGACATAG GCTACAAGAC GGCATCTCAT 
CAGTTCACCG CTTTGCCCAC CGACATGAAG GTGGAATGGG CCAAGAAGGC ATACGGTTTA 
CAGAGTGATA ACCAATACAG GGCAGATGTG AAGTGGATGA AAGGCACGGG CTGGGTCGCC 
ACCGGGTCAT TAAATGTGGA GCAGGCGAAG AAGGCAGGAG AACTCATTAG CGAGAAGAAG 
TACCGTCAGC ATCCAGATGC TTTGAAGTTT ACCAGTATTA AAGACACTCC GGAGATGGTC 
CAGGCCAGAA TTAGTTATAC CCAAGCAGTG GATAGGTTAT ACAGGGAACA AGGAGAAAAT 
ATAAAGCATC ATTACACTCA GACAGCCGAC CTCCCTGAAG TCCTGCTGGC CAAGCTGAAT 
GCTATGAATA TCAGTGAGAC GCGTTATAAG GAATCCTGGA GCAAACTTCG GGACGGTGGC 
TATAAACTGA GGTTGGATGC CATTCCGTTC CAGGCAGCAA AGGCTTCTGG CGAAATCATA 
AGTGATTACA AATACAAAGA GGCATTTGAG AAAATGAAAG GGCAGATGCT TGGCTCCCGG 
AGCTTGGAAG ACGATATCAG CCTTGCACAT TCAGTGTATG CGAACTCGCT GCAGAGCGAA 
GTGAATTACA AGAAAGGCTT TGAGCTCTCA AAGGCACACT TCCATCTGCC GTTGGACATG 
GTAACCCTGG TGCATGCCAA GAAGGCTCAG AGCCTGGCCA GCGACCAGGA CTACAGACAC 
CCGCTCCCCC AGTACACATC CTTGGCTGAA GACCTGAGGC TCAGCTGCGC CAAGAAAGCT 
CACAAGCTGC AGAGTGAGAA TTTGTACCGC TCAGACCTGA ACTTTATGCG AGGCGTTCCC 
TGTGTCATTC CGGGCACATT AGAGATTGAA GGGAGAAAGA AAGCATCTGA ACTCATCAGT 
GAGAGTAAAT ACCGTCAGCA TCCCCAGTCA TTCAAGTACA CAGCTGTGAC AGACACTCCC 
AGCCTCCTTC ATGCAAAACT CAGCAACCAG ATTACCAATG AGCGCCTCTA TAAAGCAGCC 
GGAGAGGATG AGAGGCACCG GTACACAATG ACCCTGGGTC TGCCCGAGTT TGTCCGAGCG 
AAAGCGAACG CAGCCAATCT GAGTGACGCA AAATACAAGG AGTCTTGGCG TAATCTCCGT 
GCTCAAGGCT ACAAGCTGAC AATAGACGCT CTCCCCTTCC AGGCTGCTCG CGTCTCTGGA 
GATATAGCCA GTGATTTTCT CTACAGACAT GACTTTGTGA AGGAGCGAGG CAGGCTGATC 
GGGGCCCGGA GTGTGAGTGA TGACCCCCGA CTCCAGCACT GCTGGCGGGT AGGCCAGTTG 
CAGAGCGAGC TTCAGTACAG GCGGGGAGCG GCGAGCAGCC AAGCCCACTA CCACCTGCCC 
ATGGACATGG TGCACCTGGT CCATGCCAGG AAGGCCCAGG CCCTAGCCAG CGACCACGAC 
TACAGGACAC GGTGCCATGA GTTCACAGCT CTGCCTGAGG ACCTGAAGAT GTCCTGGGCC 
AAGAGAGCTC ACGCCCTGCA GAGTGAGTTT CGCTACAAGT CAGACCTGAT GGGCATGAAG 
GGGACAGGAT GGCTGACGCT GAGTTCGCCG CAGCTAGAGA GTGCAAAGAA GGCCGGAGAA 
CTCATCAGCG AGACCAAATA CCGTAAGAAA CCAGATAGTA TCAAGTTCAC CACGGTGGTT 
GACTCCCCAG ACCTGGTTCA CGCCAAGAAC AGCTACATGC ATTGCAATGA GCGCCTGTAC 
AGATTGGGGG ATGCGGAATC CCTGCACAGA TATACGCTGA TCCCCGACCA CCCCGATTTC 
ACCAGAGCCC GCCTCAACGC GCTGCATCTG AGTGATAAAG TCTACAGAAA CTCTTGGGAA 
CAGACCCGGG CTGGTGGCTA TGACTTCCGG CTGGATGCCA TCCCATTCCA GACTGCCCGG 
GCGTCCCGGG AGATCGCCAG TGATTTCCGG TACAAAGAGG CCTTCCTGCG GGACCGGGGC 
CTACAGATTG GTTACCGCAG CATCCACGAC GACCCCAGGA TGAGGCATTT CCTCAGCGTC 
GGCAAACTCC AGAGTGACAA CGAGTACAAG AAGGACTTTG CCAAGAGTCG GTCCCAGTTC 
CATAGCCGCC CGGACCAGCC CGGCTTCCTC CAGGCCAGGA GGAGCCAGCA GCTGGCCAGC 
GACGTGTGCT ACCGGCAGCC CCTGCCCCAG CCCACATGCG ACCCGGAGCA GCTAGGCCTG 
AAGCACGCAC AGAAGGCCCA CCAGCTGCAG AGTGACGTCA AGTACAAATC AGACTTGAAC 
CTGACCAGAG GCGTTGGCTG GACACCCCCC GGCTCCTACA AAGTGGAAAT GGCTCGGCGG 
GCGGCAGAAC TGGCCAAGGC GAGGGGCCTG GGTCTCCGGG GCGCTTGGGG GGGGCCAGAG 
GCTGTGGAGC CCGCAGAGAA TCAGAGGGGG GACGTGAACC CAGACGCCAC CGAGATTCTG 
CACGTCAAAA GGAGGAAGGC TCTGCCGTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_014108945.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964420.1 Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X1, mRNA.

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CloneID	OCe43944
Clone ID Related Accession (Same CDS sequence)	XM_014108945.1 , XM_014108945.2
Accession Version	XM_014108945.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	nebulin-related-anchoring protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGCAGGA GTATACAGAA 
GACTATGAAC AACAGAGGGG CAAAGGGAGC TTTCCAGCCA TGATCACACC TGCCTATCAA 
AGAGCCAAGA AAGCTAACCA GCTGGCCAGC CAAGTGGAAT ACAAGAGAGG GCATGACGAG 
CGCATCTCCA GGTTTACCAC GGTGGCGGAT ACCCCTGAGC TGCTACGGGC CCAGGCAGGG 
GGACAGCTTC AAAGTGATGT GAGATACACA GAAGACTATG AACAACAGAG AGGAAAAGGC 
AGTTTCCCTG CTATGATCAC GCCTGCTTAT CAGATAGCCA AGAGAGCCAA TGAGCTGGCT 
AGCGATGTGA GGTACCATCA ACAATATCAA AGAGAAATGA AGGGAATGGC TAGTCCAGCT 
GCTGGAGCTG CAGGCACAAG GGAATGCATG GACCGATATG GCCAGCAGGG TTACTTGGAG 
GAATATCAGG AGCACAGAGG AAAAGGCAGC TTCCCCGCCA TGATCACCCC AGCTTATCAG 
AATGCCAAGA AAGCCAACGA ACTCGCTAGT GACATAAAAT ACAGGCAGGA CTTCAATAAG 
ATGAAAGGCC CTGCACATTA TCACTCCCTC CCAGCCCAGG ACAACTTGGT TCTCAAGCGG 
GCCCAGAGCG TGAACAAGCT TGTGAGCGAG GTGGAATATA AGAAGGACCT GGAAAGTAGT 
AAAGGTCACA GTATCAACTA CTGTGAGACG CCTCAATTCA GGAACGTGAG CAAGATCTCA 
AAATTCACCA GTGATAATAA ATATAAAGAA AATTACCAGA ACCACATGAG AGGCCACTAT 
GAAGGAGTTG GTATGGACAG ACGGACTCTC CATGCTATGA AGGTTGGAAG CTTGGCAAGC 
AATATTGCCT ACAAGGCTGA TTATAAACAC GATGTTGTGG ACTACAACTA CCCAGCCACT 
CTTACTCCCT CTTACCAGAC AACAATGAAA CTTGTCCCCT TGAAAGATGT CAACTACAGG 
CAAAGTATCG ACAAGTTGAA GTACAGCTCT GTGACCAACA CCCCTCAGAT TATCCAAGCC 
AAAATCAATG CCCAACAACT GAGTCATGTG AATTATCGTG CTGACTATGA GAAAAATAAG 
TTGAACTACA CCTTGCCACA GGATGTACCT CAGCTGGTGA AGGCCAAAAC CAATGCTGAA 
CTATTTAGTG AGGTTAAGTA TAAAGAAGGC TGGGAGAAGG CCAAGGGGAA AGGATTTGAG 
ATGAAGCTGG ATGCTATGTC TTTGCTGGCC GCCAAAGCTT CTGGAGAGCT TGCTAGCAAT 
ATTAAATATA AAGAAGAGTA TGAAAAAACG AAAGGCAAAG TCATGGGCAC TACAGACTCG 
AGGCTTCTGC ACTCTCTGCA GGTGGCCAAG ATGAGCAGTG AGGTTGAATA CAAGAAAGGC 
TTTGAAGAGA GTAAGACCCA CTTTCACCTA CCCATGGATA TGGTGCACAT CAAGCATGCT 
AAGAAGGCCC AAGCTCTGGC CAGTGACCTG GACTACAGGA AAAAGCTGCA TGAATACACT 
GTACTGCCTG AAGATATGAA GACTCAGTGG GCCAAGAAAG CCTATGGGCT CCAGAGTGAG 
CTGCAGTACA AGGCCGACCT AGCATGGATG AAAGGAGTCG GGTGGCTGAC GGAGGGGAGT 
CTCAATTTGG AACAGGCCAA GAAGGCTGGC CAGCTGGCCA GCGAGAAAAA CTACAGGCAG 
AGGGTGGATG AGCTGAAGTT CACCAGTGTG ACCGACAGCT CCCAGATGGA GCACGCCAAG 
AAGAGCCAGG AGCTGCAGAG CGGGGTGGCC TACAAGGCAG GAAATGAGCA GTCTGTCCAT 
CAGTACAGCA TCAGCAAAGA TGAACCTCTC TTCTTACAGG CCCGAGTCAA TGCCGCCAAT 
CTCAGTGAGA AACTGTATAA GAGCAGCTGG GAAAACCAGA AGGCAAAAGG TTTTGAGCTG 
CGTCTGGACT CCTTGGGCTT CCTGGCAGCC AAAGCCAACA GGGACCTGGC GAGCGAGGTG 
AAGTACAAGG AAGATTACGA GAGGTCCCGA GGTAAGCTCA TTGGAGCGAA GGATGCCCAG 
GGGGATTCAC AAATGAGCCA CTCGCTGCAA ATGTCCAAGC TGCAGAGTGA ACTCGAGTAC 
AAGAAAGGGT TCGAGGACAC CAGATCCCAG TGCCACGTCT CCCTGGACAT GATCCACCTT 
GTGCATGCCC GCAAGGCTCA GCATTTAGCC ACAGACATAG GCTACAAGAC GGCATCTCAT 
CAGTTCACCG CTTTGCCCAC CGACATGAAG GTGGAATGGG CCAAGAAGGC ATACGGTTTA 
CAGAGTGATA ACCAATACAG GGCAGATGTG AAGTGGATGA AAGGCACGGG CTGGGTCGCC 
ACCGGGTCAT TAAATGTGGA GCAGGCGAAG AAGGCAGGAG AACTCATTAG CGAGAAGAAG 
TACCGTCAGC ATCCAGATGC TTTGAAGTTT ACCAGTATTA AAGACACTCC GGAGATGGTC 
CAGGCCAGAA TTAGTTATAC CCAAGCAGTG GATAGGTTAT ACAGGGAACA AGGAGAAAAT 
ATAAAGCATC ATTACACTCA GACAGCCGAC CTCCCTGAAG TCCTGCTGGC CAAGCTGAAT 
GCTATGAATA TCAGTGAGAC GCGTTATAAG GAATCCTGGA GCAAACTTCG GGACGGTGGC 
TATAAACTGA GGTTGGATGC CATTCCGTTC CAGGCAGCAA AGGCTTCTGG CGAAATCATA 
AGTGATTACA AATACAAAGA GGCATTTGAG AAAATGAAAG GGCAGATGCT TGGCTCCCGG 
AGCTTGGAAG ACGATATCAG CCTTGCACAT TCAGTGTATG CGAACTCGCT GCAGAGCGAA 
GTGAATTACA AGAAAGGCTT TGAGCTCTCA AAGGCACACT TCCATCTGCC GTTGGACATG 
GTAACCCTGG TGCATGCCAA GAAGGCTCAG AGCCTGGCCA GCGACCAGGA CTACAGACAC 
CCGCTCCCCC AGTACACATC CTTGGCTGAA GACCTGAGGC TCAGCTGCGC CAAGAAAGCT 
CACAAGCTGC AGAGTGAGAA TTTGTACCGC TCAGACCTGA ACTTTATGCG AGGCGTTCCC 
TGTGTCATTC CGGGCACATT AGAGATTGAA GGGAGAAAGA AAGCATCTGA ACTCATCAGT 
GAGAGTAAAT ACCGTCAGCA TCCCCAGTCA TTCAAGTACA CAGCTGTGAC AGACACTCCC 
AGCCTCCTTC ATGCAAAACT CAGCAACCAG ATTACCAATG AGCGCCTCTA TAAAGCAGCC 
GGAGAGGATG AGAGGCACCG GTACACAATG ACCCTGGGTC TGCCCGAGTT TGTCCGAGCG 
AAAGCGAACG CAGCCAATCT GAGTGACGCA AAATACAAGG AGTCTTGGCG TAATCTCCGT 
GCTCAAGGCT ACAAGCTGAC AATAGACGCT CTCCCCTTCC AGGCTGCTCG CGTCTCTGGA 
GATATAGCCA GTGATTTTCT CTACAGACAT GACTTTGTGA AGGAGCGAGG CAGGCTGATC 
GGGGCCCGGA GTGTGAGTGA TGACCCCCGA CTCCAGCACT GCTGGCGGGT AGGCCAGTTG 
CAGAGCGAGC TTCAGTACAG GCGGGGAGCG GCGAGCAGCC AAGCCCACTA CCACCTGCCC 
ATGGACATGG TGCACCTGGT CCATGCCAGG AAGGCCCAGG CCCTAGCCAG CGACCACGAC 
TACAGGACAC GGTGCCATGA GTTCACAGCT CTGCCTGAGG ACCTGAAGAT GTCCTGGGCC 
AAGAGAGCTC ACGCCCTGCA GAGTGAGTTT CGCTACAAGT CAGACCTGAT GGGCATGAAG 
GGGACAGGAT GGCTGACGCT GAGTTCGCCG CAGCTAGAGA GTGCAAAGAA GGCCGGAGAA 
CTCATCAGCG AGACCAAATA CCGTAAGAAA CCAGATAGTA TCAAGTTCAC CACGGTGGTT 
GACTCCCCAG ACCTGGTTCA CGCCAAGAAC AGCTACATGC ATTGCAATGA GCGCCTGTAC 
AGATTGGGGG ATGCGGAATC CCTGCACAGA TATACGCTGA TCCCCGACCA CCCCGATTTC 
ACCAGAGCCC GCCTCAACGC GCTGCATCTG AGTGATAAAG TCTACAGAAA CTCTTGGGAA 
CAGACCCGGG CTGGTGGCTA TGACTTCCGG CTGGATGCCA TCCCATTCCA GACTGCCCGG 
GCGTCCCGGG AGATCGCCAG TGATTTCCGG TACAAAGAGG CCTTCCTGCG GGACCGGGGC 
CTACAGATTG GTTACCGCAG CATCCACGAC GACCCCAGGA TGAGGCATTT CCTCAGCGTC 
GGCAAACTCC AGAGTGACAA CGAGTACAAG AAGGACTTTG CCAAGAGTCG GTCCCAGTTC 
CATAGCCGCC CGGACCAGCC CGGCTTCCTC CAGGCCAGGA GGAGCCAGCA GCTGGCCAGC 
GACGTGTGCT ACCGGCAGCC CCTGCCCCAG CCCACATGCG ACCCGGAGCA GCTAGGCCTG 
AAGCACGCAC AGAAGGCCCA CCAGCTGCAG AGTGACGTCA AGTACAAATC AGACTTGAAC 
CTGACCAGAG GCGTTGGCTG GACACCCCCC GGCTCCTACA AAGTGGAAAT GGCTCGGCGG 
GCGGCAGAAC TGGCCAAGGC GAGGGGCCTG GGTCTCCGGG GCGCTTGGGG GGGGCCAGAG 
GCTGTGGAGC CCGCAGAGAA TCAGAGGGGG GACGTGAACC CAGACGCCAC CGAGATTCTG 
CACGTCAAAA GGAGGAAGGC TCTGCCGTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964420.1
CDS	225..5417
Translation	MNVQACSRCGYGVYPAEKISCLDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRKSPETIHGIDEQDEGERFKSVFHWDMKSRDGTAAPNRQPLMNERAYWNGYGEGNAWCPGAMPDPEIVRMVETRKSLGEQEYTEDYEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKRGHDERISRFTTVADTPELLRAQAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMKGMASPAAGAAGTRECMDRYGQQGYLEEYQEHRGKGSFPAMITPAYQNAKKANELASDIKYRQDFNKMKGPAHYHSLPAQDNLVLKRAQSVNKLVSEVEYKKDLESSKGHSINYCETPQFRNVSKISKFTSDNKYKENYQNHMRGHYEGVGMDRRTLHAMKVGSLASNIAYKADYKHDVVDYNYPATLTPSYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIIQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAELFSEVKYKEGWEKAKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKVMGTTDSRLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVHIKHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLASEKNYRQRVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYSISKDEPLFLQARVNAANLSEKLYKSSWENQKAKGFELRLDSLGFLAAKANRDLASEVKYKEDYERSRGKLIGAKDAQGDSQMSHSLQMSKLQSELEYKKGFEDTRSQCHVSLDMIHLVHARKAQHLATDIGYKTASHQFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTADLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASGEIISDYKYKEAFEKMKGQMLGSRSLEDDISLAHSVYANSLQSEVNYKKGFELSKAHFHLPLDMVTLVHAKKAQSLASDQDYRHPLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVPCVIPGTLEIEGRKKASELISESKYRQHPQSFKYTAVTDTPSLLHAKLSNQITNERLYKAAGEDERHRYTMTLGLPEFVRAKANAANLSDAKYKESWRNLRAQGYKLTIDALPFQAARVSGDIASDFLYRHDFVKERGRLIGARSVSDDPRLQHCWRVGQLQSELQYRRGAASSQAHYHLPMDMVHLVHARKAQALASDHDYRTRCHEFTALPEDLKMSWAKRAHALQSEFRYKSDLMGMKGTGWLTLSSPQLESAKKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERLYRLGDAESLHRYTLIPDHPDFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGYRSIHDDPRMRHFLSVGKLQSDNEYKKDFAKSRSQFHSRPDQPGFLQARRSQQLASDVCYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELAKARGLGLRGAWGGPEAVEPAENQRGDVNPDATEILHVKRRKALPL

Target ORF information:

RefSeq Version	XM_014108945.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108945.2

ORF Insert Sequence:

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGCAGGA GTATACAGAA 
GACTATGAAC AACAGAGGGG CAAAGGGAGC TTTCCAGCCA TGATCACACC TGCCTATCAA 
AGAGCCAAGA AAGCTAACCA GCTGGCCAGC CAAGTGGAAT ACAAGAGAGG GCATGACGAG 
CGCATCTCCA GGTTTACCAC GGTGGCGGAT ACCCCTGAGC TGCTACGGGC CCAGGCAGGG 
GGACAGCTTC AAAGTGATGT GAGATACACA GAAGACTATG AACAACAGAG AGGAAAAGGC 
AGTTTCCCTG CTATGATCAC GCCTGCTTAT CAGATAGCCA AGAGAGCCAA TGAGCTGGCT 
AGCGATGTGA GGTACCATCA ACAATATCAA AGAGAAATGA AGGGAATGGC TAGTCCAGCT 
GCTGGAGCTG CAGGCACAAG GGAATGCATG GACCGATATG GCCAGCAGGG TTACTTGGAG 
GAATATCAGG AGCACAGAGG AAAAGGCAGC TTCCCCGCCA TGATCACCCC AGCTTATCAG 
AATGCCAAGA AAGCCAACGA ACTCGCTAGT GACATAAAAT ACAGGCAGGA CTTCAATAAG 
ATGAAAGGCC CTGCACATTA TCACTCCCTC CCAGCCCAGG ACAACTTGGT TCTCAAGCGG 
GCCCAGAGCG TGAACAAGCT TGTGAGCGAG GTGGAATATA AGAAGGACCT GGAAAGTAGT 
AAAGGTCACA GTATCAACTA CTGTGAGACG CCTCAATTCA GGAACGTGAG CAAGATCTCA 
AAATTCACCA GTGATAATAA ATATAAAGAA AATTACCAGA ACCACATGAG AGGCCACTAT 
GAAGGAGTTG GTATGGACAG ACGGACTCTC CATGCTATGA AGGTTGGAAG CTTGGCAAGC 
AATATTGCCT ACAAGGCTGA TTATAAACAC GATGTTGTGG ACTACAACTA CCCAGCCACT 
CTTACTCCCT CTTACCAGAC AACAATGAAA CTTGTCCCCT TGAAAGATGT CAACTACAGG 
CAAAGTATCG ACAAGTTGAA GTACAGCTCT GTGACCAACA CCCCTCAGAT TATCCAAGCC 
AAAATCAATG CCCAACAACT GAGTCATGTG AATTATCGTG CTGACTATGA GAAAAATAAG 
TTGAACTACA CCTTGCCACA GGATGTACCT CAGCTGGTGA AGGCCAAAAC CAATGCTGAA 
CTATTTAGTG AGGTTAAGTA TAAAGAAGGC TGGGAGAAGG CCAAGGGGAA AGGATTTGAG 
ATGAAGCTGG ATGCTATGTC TTTGCTGGCC GCCAAAGCTT CTGGAGAGCT TGCTAGCAAT 
ATTAAATATA AAGAAGAGTA TGAAAAAACG AAAGGCAAAG TCATGGGCAC TACAGACTCG 
AGGCTTCTGC ACTCTCTGCA GGTGGCCAAG ATGAGCAGTG AGGTTGAATA CAAGAAAGGC 
TTTGAAGAGA GTAAGACCCA CTTTCACCTA CCCATGGATA TGGTGCACAT CAAGCATGCT 
AAGAAGGCCC AAGCTCTGGC CAGTGACCTG GACTACAGGA AAAAGCTGCA TGAATACACT 
GTACTGCCTG AAGATATGAA GACTCAGTGG GCCAAGAAAG CCTATGGGCT CCAGAGTGAG 
CTGCAGTACA AGGCCGACCT AGCATGGATG AAAGGAGTCG GGTGGCTGAC GGAGGGGAGT 
CTCAATTTGG AACAGGCCAA GAAGGCTGGC CAGCTGGCCA GCGAGAAAAA CTACAGGCAG 
AGGGTGGATG AGCTGAAGTT CACCAGTGTG ACCGACAGCT CCCAGATGGA GCACGCCAAG 
AAGAGCCAGG AGCTGCAGAG CGGGGTGGCC TACAAGGCAG GAAATGAGCA GTCTGTCCAT 
CAGTACAGCA TCAGCAAAGA TGAACCTCTC TTCTTACAGG CCCGAGTCAA TGCCGCCAAT 
CTCAGTGAGA AACTGTATAA GAGCAGCTGG GAAAACCAGA AGGCAAAAGG TTTTGAGCTG 
CGTCTGGACT CCTTGGGCTT CCTGGCAGCC AAAGCCAACA GGGACCTGGC GAGCGAGGTG 
AAGTACAAGG AAGATTACGA GAGGTCCCGA GGTAAGCTCA TTGGAGCGAA GGATGCCCAG 
GGGGATTCAC AAATGAGCCA CTCGCTGCAA ATGTCCAAGC TGCAGAGTGA ACTCGAGTAC 
AAGAAAGGGT TCGAGGACAC CAGATCCCAG TGCCACGTCT CCCTGGACAT GATCCACCTT 
GTGCATGCCC GCAAGGCTCA GCATTTAGCC ACAGACATAG GCTACAAGAC GGCATCTCAT 
CAGTTCACCG CTTTGCCCAC CGACATGAAG GTGGAATGGG CCAAGAAGGC ATACGGTTTA 
CAGAGTGATA ACCAATACAG GGCAGATGTG AAGTGGATGA AAGGCACGGG CTGGGTCGCC 
ACCGGGTCAT TAAATGTGGA GCAGGCGAAG AAGGCAGGAG AACTCATTAG CGAGAAGAAG 
TACCGTCAGC ATCCAGATGC TTTGAAGTTT ACCAGTATTA AAGACACTCC GGAGATGGTC 
CAGGCCAGAA TTAGTTATAC CCAAGCAGTG GATAGGTTAT ACAGGGAACA AGGAGAAAAT 
ATAAAGCATC ATTACACTCA GACAGCCGAC CTCCCTGAAG TCCTGCTGGC CAAGCTGAAT 
GCTATGAATA TCAGTGAGAC GCGTTATAAG GAATCCTGGA GCAAACTTCG GGACGGTGGC 
TATAAACTGA GGTTGGATGC CATTCCGTTC CAGGCAGCAA AGGCTTCTGG CGAAATCATA 
AGTGATTACA AATACAAAGA GGCATTTGAG AAAATGAAAG GGCAGATGCT TGGCTCCCGG 
AGCTTGGAAG ACGATATCAG CCTTGCACAT TCAGTGTATG CGAACTCGCT GCAGAGCGAA 
GTGAATTACA AGAAAGGCTT TGAGCTCTCA AAGGCACACT TCCATCTGCC GTTGGACATG 
GTAACCCTGG TGCATGCCAA GAAGGCTCAG AGCCTGGCCA GCGACCAGGA CTACAGACAC 
CCGCTCCCCC AGTACACATC CTTGGCTGAA GACCTGAGGC TCAGCTGCGC CAAGAAAGCT 
CACAAGCTGC AGAGTGAGAA TTTGTACCGC TCAGACCTGA ACTTTATGCG AGGCGTTCCC 
TGTGTCATTC CGGGCACATT AGAGATTGAA GGGAGAAAGA AAGCATCTGA ACTCATCAGT 
GAGAGTAAAT ACCGTCAGCA TCCCCAGTCA TTCAAGTACA CAGCTGTGAC AGACACTCCC 
AGCCTCCTTC ATGCAAAACT CAGCAACCAG ATTACCAATG AGCGCCTCTA TAAAGCAGCC 
GGAGAGGATG AGAGGCACCG GTACACAATG ACCCTGGGTC TGCCCGAGTT TGTCCGAGCG 
AAAGCGAACG CAGCCAATCT GAGTGACGCA AAATACAAGG AGTCTTGGCG TAATCTCCGT 
GCTCAAGGCT ACAAGCTGAC AATAGACGCT CTCCCCTTCC AGGCTGCTCG CGTCTCTGGA 
GATATAGCCA GTGATTTTCT CTACAGACAT GACTTTGTGA AGGAGCGAGG CAGGCTGATC 
GGGGCCCGGA GTGTGAGTGA TGACCCCCGA CTCCAGCACT GCTGGCGGGT AGGCCAGTTG 
CAGAGCGAGC TTCAGTACAG GCGGGGAGCG GCGAGCAGCC AAGCCCACTA CCACCTGCCC 
ATGGACATGG TGCACCTGGT CCATGCCAGG AAGGCCCAGG CCCTAGCCAG CGACCACGAC 
TACAGGACAC GGTGCCATGA GTTCACAGCT CTGCCTGAGG ACCTGAAGAT GTCCTGGGCC 
AAGAGAGCTC ACGCCCTGCA GAGTGAGTTT CGCTACAAGT CAGACCTGAT GGGCATGAAG 
GGGACAGGAT GGCTGACGCT GAGTTCGCCG CAGCTAGAGA GTGCAAAGAA GGCCGGAGAA 
CTCATCAGCG AGACCAAATA CCGTAAGAAA CCAGATAGTA TCAAGTTCAC CACGGTGGTT 
GACTCCCCAG ACCTGGTTCA CGCCAAGAAC AGCTACATGC ATTGCAATGA GCGCCTGTAC 
AGATTGGGGG ATGCGGAATC CCTGCACAGA TATACGCTGA TCCCCGACCA CCCCGATTTC 
ACCAGAGCCC GCCTCAACGC GCTGCATCTG AGTGATAAAG TCTACAGAAA CTCTTGGGAA 
CAGACCCGGG CTGGTGGCTA TGACTTCCGG CTGGATGCCA TCCCATTCCA GACTGCCCGG 
GCGTCCCGGG AGATCGCCAG TGATTTCCGG TACAAAGAGG CCTTCCTGCG GGACCGGGGC 
CTACAGATTG GTTACCGCAG CATCCACGAC GACCCCAGGA TGAGGCATTT CCTCAGCGTC 
GGCAAACTCC AGAGTGACAA CGAGTACAAG AAGGACTTTG CCAAGAGTCG GTCCCAGTTC 
CATAGCCGCC CGGACCAGCC CGGCTTCCTC CAGGCCAGGA GGAGCCAGCA GCTGGCCAGC 
GACGTGTGCT ACCGGCAGCC CCTGCCCCAG CCCACATGCG ACCCGGAGCA GCTAGGCCTG 
AAGCACGCAC AGAAGGCCCA CCAGCTGCAG AGTGACGTCA AGTACAAATC AGACTTGAAC 
CTGACCAGAG GCGTTGGCTG GACACCCCCC GGCTCCTACA AAGTGGAAAT GGCTCGGCGG 
GCGGCAGAAC TGGCCAAGGC GAGGGGCCTG GGTCTCCGGG GCGCTTGGGG GGGGCCAGAG 
GCTGTGGAGC CCGCAGAGAA TCAGAGGGGG GACGTGAACC CAGACGCCAC CGAGATTCTG 
CACGTCAAAA GGAGGAAGGC TCTGCCGTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_014108946.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964421.1 Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X2, mRNA.

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CloneID	OCe43945
Clone ID Related Accession (Same CDS sequence)	XM_014108946.1 , XM_014108946.2
Accession Version	XM_014108946.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	nebulin-related-anchoring protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGCAGGA GTATACAGAA 
GACTATGAAC AACAGAGGGG CAAAGGGAGC TTTCCAGCCA TGATCACACC TGCCTATCAA 
AGAGCCAAGA AAGCTAACCA GCTGGCCAGC CAAGTGGAAT ACAAGAGAGG GCATGACGAG 
CGCATCTCCA GGTTTACCAC GGTGGCGGAT ACCCCTGAGC TGCTACGGGC CCAGGCAGGG 
GGACAGCTTC AAAGTGATGT GAGATACACA GAAGACTATG AACAACAGAG AGGAAAAGGC 
AGTTTCCCTG CTATGATCAC GCCTGCTTAT CAGATAGCCA AGAGAGCCAA TGAGCTGGCT 
AGCGATGTGA GGTACCATCA ACAATATCAA AGAGAAATGA AGGGAATGGC TAGTCCAGCT 
GCTGGAGCTG CAGGCACAAG GGAATGCATG GACCGATATG GCCAGGGTTA CTTGGAGGAA 
TATCAGGAGC ACAGAGGAAA AGGCAGCTTC CCCGCCATGA TCACCCCAGC TTATCAGAAT 
GCCAAGAAAG CCAACGAACT CGCTAGTGAC ATAAAATACA GGCAGGACTT CAATAAGATG 
AAAGGCCCTG CACATTATCA CTCCCTCCCA GCCCAGGACA ACTTGGTTCT CAAGCGGGCC 
CAGAGCGTGA ACAAGCTTGT GAGCGAGGTG GAATATAAGA AGGACCTGGA AAGTAGTAAA 
GGTCACAGTA TCAACTACTG TGAGACGCCT CAATTCAGGA ACGTGAGCAA GATCTCAAAA 
TTCACCAGTG ATAATAAATA TAAAGAAAAT TACCAGAACC ACATGAGAGG CCACTATGAA 
GGAGTTGGTA TGGACAGACG GACTCTCCAT GCTATGAAGG TTGGAAGCTT GGCAAGCAAT 
ATTGCCTACA AGGCTGATTA TAAACACGAT GTTGTGGACT ACAACTACCC AGCCACTCTT 
ACTCCCTCTT ACCAGACAAC AATGAAACTT GTCCCCTTGA AAGATGTCAA CTACAGGCAA 
AGTATCGACA AGTTGAAGTA CAGCTCTGTG ACCAACACCC CTCAGATTAT CCAAGCCAAA 
ATCAATGCCC AACAACTGAG TCATGTGAAT TATCGTGCTG ACTATGAGAA AAATAAGTTG 
AACTACACCT TGCCACAGGA TGTACCTCAG CTGGTGAAGG CCAAAACCAA TGCTGAACTA 
TTTAGTGAGG TTAAGTATAA AGAAGGCTGG GAGAAGGCCA AGGGGAAAGG ATTTGAGATG 
AAGCTGGATG CTATGTCTTT GCTGGCCGCC AAAGCTTCTG GAGAGCTTGC TAGCAATATT 
AAATATAAAG AAGAGTATGA AAAAACGAAA GGCAAAGTCA TGGGCACTAC AGACTCGAGG 
CTTCTGCACT CTCTGCAGGT GGCCAAGATG AGCAGTGAGG TTGAATACAA GAAAGGCTTT 
GAAGAGAGTA AGACCCACTT TCACCTACCC ATGGATATGG TGCACATCAA GCATGCTAAG 
AAGGCCCAAG CTCTGGCCAG TGACCTGGAC TACAGGAAAA AGCTGCATGA ATACACTGTA 
CTGCCTGAAG ATATGAAGAC TCAGTGGGCC AAGAAAGCCT ATGGGCTCCA GAGTGAGCTG 
CAGTACAAGG CCGACCTAGC ATGGATGAAA GGAGTCGGGT GGCTGACGGA GGGGAGTCTC 
AATTTGGAAC AGGCCAAGAA GGCTGGCCAG CTGGCCAGCG AGAAAAACTA CAGGCAGAGG 
GTGGATGAGC TGAAGTTCAC CAGTGTGACC GACAGCTCCC AGATGGAGCA CGCCAAGAAG 
AGCCAGGAGC TGCAGAGCGG GGTGGCCTAC AAGGCAGGAA ATGAGCAGTC TGTCCATCAG 
TACAGCATCA GCAAAGATGA ACCTCTCTTC TTACAGGCCC GAGTCAATGC CGCCAATCTC 
AGTGAGAAAC TGTATAAGAG CAGCTGGGAA AACCAGAAGG CAAAAGGTTT TGAGCTGCGT 
CTGGACTCCT TGGGCTTCCT GGCAGCCAAA GCCAACAGGG ACCTGGCGAG CGAGGTGAAG 
TACAAGGAAG ATTACGAGAG GTCCCGAGGT AAGCTCATTG GAGCGAAGGA TGCCCAGGGG 
GATTCACAAA TGAGCCACTC GCTGCAAATG TCCAAGCTGC AGAGTGAACT CGAGTACAAG 
AAAGGGTTCG AGGACACCAG ATCCCAGTGC CACGTCTCCC TGGACATGAT CCACCTTGTG 
CATGCCCGCA AGGCTCAGCA TTTAGCCACA GACATAGGCT ACAAGACGGC ATCTCATCAG 
TTCACCGCTT TGCCCACCGA CATGAAGGTG GAATGGGCCA AGAAGGCATA CGGTTTACAG 
AGTGATAACC AATACAGGGC AGATGTGAAG TGGATGAAAG GCACGGGCTG GGTCGCCACC 
GGGTCATTAA ATGTGGAGCA GGCGAAGAAG GCAGGAGAAC TCATTAGCGA GAAGAAGTAC 
CGTCAGCATC CAGATGCTTT GAAGTTTACC AGTATTAAAG ACACTCCGGA GATGGTCCAG 
GCCAGAATTA GTTATACCCA AGCAGTGGAT AGGTTATACA GGGAACAAGG AGAAAATATA 
AAGCATCATT ACACTCAGAC AGCCGACCTC CCTGAAGTCC TGCTGGCCAA GCTGAATGCT 
ATGAATATCA GTGAGACGCG TTATAAGGAA TCCTGGAGCA AACTTCGGGA CGGTGGCTAT 
AAACTGAGGT TGGATGCCAT TCCGTTCCAG GCAGCAAAGG CTTCTGGCGA AATCATAAGT 
GATTACAAAT ACAAAGAGGC ATTTGAGAAA ATGAAAGGGC AGATGCTTGG CTCCCGGAGC 
TTGGAAGACG ATATCAGCCT TGCACATTCA GTGTATGCGA ACTCGCTGCA GAGCGAAGTG 
AATTACAAGA AAGGCTTTGA GCTCTCAAAG GCACACTTCC ATCTGCCGTT GGACATGGTA 
ACCCTGGTGC ATGCCAAGAA GGCTCAGAGC CTGGCCAGCG ACCAGGACTA CAGACACCCG 
CTCCCCCAGT ACACATCCTT GGCTGAAGAC CTGAGGCTCA GCTGCGCCAA GAAAGCTCAC 
AAGCTGCAGA GTGAGAATTT GTACCGCTCA GACCTGAACT TTATGCGAGG CGTTCCCTGT 
GTCATTCCGG GCACATTAGA GATTGAAGGG AGAAAGAAAG CATCTGAACT CATCAGTGAG 
AGTAAATACC GTCAGCATCC CCAGTCATTC AAGTACACAG CTGTGACAGA CACTCCCAGC 
CTCCTTCATG CAAAACTCAG CAACCAGATT ACCAATGAGC GCCTCTATAA AGCAGCCGGA 
GAGGATGAGA GGCACCGGTA CACAATGACC CTGGGTCTGC CCGAGTTTGT CCGAGCGAAA 
GCGAACGCAG CCAATCTGAG TGACGCAAAA TACAAGGAGT CTTGGCGTAA TCTCCGTGCT 
CAAGGCTACA AGCTGACAAT AGACGCTCTC CCCTTCCAGG CTGCTCGCGT CTCTGGAGAT 
ATAGCCAGTG ATTTTCTCTA CAGACATGAC TTTGTGAAGG AGCGAGGCAG GCTGATCGGG 
GCCCGGAGTG TGAGTGATGA CCCCCGACTC CAGCACTGCT GGCGGGTAGG CCAGTTGCAG 
AGCGAGCTTC AGTACAGGCG GGGAGCGGCG AGCAGCCAAG CCCACTACCA CCTGCCCATG 
GACATGGTGC ACCTGGTCCA TGCCAGGAAG GCCCAGGCCC TAGCCAGCGA CCACGACTAC 
AGGACACGGT GCCATGAGTT CACAGCTCTG CCTGAGGACC TGAAGATGTC CTGGGCCAAG 
AGAGCTCACG CCCTGCAGAG TGAGTTTCGC TACAAGTCAG ACCTGATGGG CATGAAGGGG 
ACAGGATGGC TGACGCTGAG TTCGCCGCAG CTAGAGAGTG CAAAGAAGGC CGGAGAACTC 
ATCAGCGAGA CCAAATACCG TAAGAAACCA GATAGTATCA AGTTCACCAC GGTGGTTGAC 
TCCCCAGACC TGGTTCACGC CAAGAACAGC TACATGCATT GCAATGAGCG CCTGTACAGA 
TTGGGGGATG CGGAATCCCT GCACAGATAT ACGCTGATCC CCGACCACCC CGATTTCACC 
AGAGCCCGCC TCAACGCGCT GCATCTGAGT GATAAAGTCT ACAGAAACTC TTGGGAACAG 
ACCCGGGCTG GTGGCTATGA CTTCCGGCTG GATGCCATCC CATTCCAGAC TGCCCGGGCG 
TCCCGGGAGA TCGCCAGTGA TTTCCGGTAC AAAGAGGCCT TCCTGCGGGA CCGGGGCCTA 
CAGATTGGTT ACCGCAGCAT CCACGACGAC CCCAGGATGA GGCATTTCCT CAGCGTCGGC 
AAACTCCAGA GTGACAACGA GTACAAGAAG GACTTTGCCA AGAGTCGGTC CCAGTTCCAT 
AGCCGCCCGG ACCAGCCCGG CTTCCTCCAG GCCAGGAGGA GCCAGCAGCT GGCCAGCGAC 
GTGTGCTACC GGCAGCCCCT GCCCCAGCCC ACATGCGACC CGGAGCAGCT AGGCCTGAAG 
CACGCACAGA AGGCCCACCA GCTGCAGAGT GACGTCAAGT ACAAATCAGA CTTGAACCTG 
ACCAGAGGCG TTGGCTGGAC ACCCCCCGGC TCCTACAAAG TGGAAATGGC TCGGCGGGCG 
GCAGAACTGG CCAAGGCGAG GGGCCTGGGT CTCCGGGGCG CTTGGGGGGG GCCAGAGGCT 
GTGGAGCCCG CAGAGAATCA GAGGGGGGAC GTGAACCCAG ACGCCACCGA GATTCTGCAC 
GTCAAAAGGA GGAAGGCTCT GCCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964421.1
CDS	203..5392
Translation	MNVQACSRCGYGVYPAEKISCLDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRKSPETIHGIDEQDEGERFKSVFHWDMKSRDGTAAPNRQPLMNERAYWNGYGEGNAWCPGAMPDPEIVRMVETRKSLGEQEYTEDYEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKRGHDERISRFTTVADTPELLRAQAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMKGMASPAAGAAGTRECMDRYGQGYLEEYQEHRGKGSFPAMITPAYQNAKKANELASDIKYRQDFNKMKGPAHYHSLPAQDNLVLKRAQSVNKLVSEVEYKKDLESSKGHSINYCETPQFRNVSKISKFTSDNKYKENYQNHMRGHYEGVGMDRRTLHAMKVGSLASNIAYKADYKHDVVDYNYPATLTPSYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIIQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAELFSEVKYKEGWEKAKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKVMGTTDSRLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVHIKHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLASEKNYRQRVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYSISKDEPLFLQARVNAANLSEKLYKSSWENQKAKGFELRLDSLGFLAAKANRDLASEVKYKEDYERSRGKLIGAKDAQGDSQMSHSLQMSKLQSELEYKKGFEDTRSQCHVSLDMIHLVHARKAQHLATDIGYKTASHQFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTADLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASGEIISDYKYKEAFEKMKGQMLGSRSLEDDISLAHSVYANSLQSEVNYKKGFELSKAHFHLPLDMVTLVHAKKAQSLASDQDYRHPLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVPCVIPGTLEIEGRKKASELISESKYRQHPQSFKYTAVTDTPSLLHAKLSNQITNERLYKAAGEDERHRYTMTLGLPEFVRAKANAANLSDAKYKESWRNLRAQGYKLTIDALPFQAARVSGDIASDFLYRHDFVKERGRLIGARSVSDDPRLQHCWRVGQLQSELQYRRGAASSQAHYHLPMDMVHLVHARKAQALASDHDYRTRCHEFTALPEDLKMSWAKRAHALQSEFRYKSDLMGMKGTGWLTLSSPQLESAKKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERLYRLGDAESLHRYTLIPDHPDFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGYRSIHDDPRMRHFLSVGKLQSDNEYKKDFAKSRSQFHSRPDQPGFLQARRSQQLASDVCYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELAKARGLGLRGAWGGPEAVEPAENQRGDVNPDATEILHVKRRKALPL

Target ORF information:

RefSeq Version	XM_014108946.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108946.1

ORF Insert Sequence:

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGCAGGA GTATACAGAA 
GACTATGAAC AACAGAGGGG CAAAGGGAGC TTTCCAGCCA TGATCACACC TGCCTATCAA 
AGAGCCAAGA AAGCTAACCA GCTGGCCAGC CAAGTGGAAT ACAAGAGAGG GCATGACGAG 
CGCATCTCCA GGTTTACCAC GGTGGCGGAT ACCCCTGAGC TGCTACGGGC CCAGGCAGGG 
GGACAGCTTC AAAGTGATGT GAGATACACA GAAGACTATG AACAACAGAG AGGAAAAGGC 
AGTTTCCCTG CTATGATCAC GCCTGCTTAT CAGATAGCCA AGAGAGCCAA TGAGCTGGCT 
AGCGATGTGA GGTACCATCA ACAATATCAA AGAGAAATGA AGGGAATGGC TAGTCCAGCT 
GCTGGAGCTG CAGGCACAAG GGAATGCATG GACCGATATG GCCAGGGTTA CTTGGAGGAA 
TATCAGGAGC ACAGAGGAAA AGGCAGCTTC CCCGCCATGA TCACCCCAGC TTATCAGAAT 
GCCAAGAAAG CCAACGAACT CGCTAGTGAC ATAAAATACA GGCAGGACTT CAATAAGATG 
AAAGGCCCTG CACATTATCA CTCCCTCCCA GCCCAGGACA ACTTGGTTCT CAAGCGGGCC 
CAGAGCGTGA ACAAGCTTGT GAGCGAGGTG GAATATAAGA AGGACCTGGA AAGTAGTAAA 
GGTCACAGTA TCAACTACTG TGAGACGCCT CAATTCAGGA ACGTGAGCAA GATCTCAAAA 
TTCACCAGTG ATAATAAATA TAAAGAAAAT TACCAGAACC ACATGAGAGG CCACTATGAA 
GGAGTTGGTA TGGACAGACG GACTCTCCAT GCTATGAAGG TTGGAAGCTT GGCAAGCAAT 
ATTGCCTACA AGGCTGATTA TAAACACGAT GTTGTGGACT ACAACTACCC AGCCACTCTT 
ACTCCCTCTT ACCAGACAAC AATGAAACTT GTCCCCTTGA AAGATGTCAA CTACAGGCAA 
AGTATCGACA AGTTGAAGTA CAGCTCTGTG ACCAACACCC CTCAGATTAT CCAAGCCAAA 
ATCAATGCCC AACAACTGAG TCATGTGAAT TATCGTGCTG ACTATGAGAA AAATAAGTTG 
AACTACACCT TGCCACAGGA TGTACCTCAG CTGGTGAAGG CCAAAACCAA TGCTGAACTA 
TTTAGTGAGG TTAAGTATAA AGAAGGCTGG GAGAAGGCCA AGGGGAAAGG ATTTGAGATG 
AAGCTGGATG CTATGTCTTT GCTGGCCGCC AAAGCTTCTG GAGAGCTTGC TAGCAATATT 
AAATATAAAG AAGAGTATGA AAAAACGAAA GGCAAAGTCA TGGGCACTAC AGACTCGAGG 
CTTCTGCACT CTCTGCAGGT GGCCAAGATG AGCAGTGAGG TTGAATACAA GAAAGGCTTT 
GAAGAGAGTA AGACCCACTT TCACCTACCC ATGGATATGG TGCACATCAA GCATGCTAAG 
AAGGCCCAAG CTCTGGCCAG TGACCTGGAC TACAGGAAAA AGCTGCATGA ATACACTGTA 
CTGCCTGAAG ATATGAAGAC TCAGTGGGCC AAGAAAGCCT ATGGGCTCCA GAGTGAGCTG 
CAGTACAAGG CCGACCTAGC ATGGATGAAA GGAGTCGGGT GGCTGACGGA GGGGAGTCTC 
AATTTGGAAC AGGCCAAGAA GGCTGGCCAG CTGGCCAGCG AGAAAAACTA CAGGCAGAGG 
GTGGATGAGC TGAAGTTCAC CAGTGTGACC GACAGCTCCC AGATGGAGCA CGCCAAGAAG 
AGCCAGGAGC TGCAGAGCGG GGTGGCCTAC AAGGCAGGAA ATGAGCAGTC TGTCCATCAG 
TACAGCATCA GCAAAGATGA ACCTCTCTTC TTACAGGCCC GAGTCAATGC CGCCAATCTC 
AGTGAGAAAC TGTATAAGAG CAGCTGGGAA AACCAGAAGG CAAAAGGTTT TGAGCTGCGT 
CTGGACTCCT TGGGCTTCCT GGCAGCCAAA GCCAACAGGG ACCTGGCGAG CGAGGTGAAG 
TACAAGGAAG ATTACGAGAG GTCCCGAGGT AAGCTCATTG GAGCGAAGGA TGCCCAGGGG 
GATTCACAAA TGAGCCACTC GCTGCAAATG TCCAAGCTGC AGAGTGAACT CGAGTACAAG 
AAAGGGTTCG AGGACACCAG ATCCCAGTGC CACGTCTCCC TGGACATGAT CCACCTTGTG 
CATGCCCGCA AGGCTCAGCA TTTAGCCACA GACATAGGCT ACAAGACGGC ATCTCATCAG 
TTCACCGCTT TGCCCACCGA CATGAAGGTG GAATGGGCCA AGAAGGCATA CGGTTTACAG 
AGTGATAACC AATACAGGGC AGATGTGAAG TGGATGAAAG GCACGGGCTG GGTCGCCACC 
GGGTCATTAA ATGTGGAGCA GGCGAAGAAG GCAGGAGAAC TCATTAGCGA GAAGAAGTAC 
CGTCAGCATC CAGATGCTTT GAAGTTTACC AGTATTAAAG ACACTCCGGA GATGGTCCAG 
GCCAGAATTA GTTATACCCA AGCAGTGGAT AGGTTATACA GGGAACAAGG AGAAAATATA 
AAGCATCATT ACACTCAGAC AGCCGACCTC CCTGAAGTCC TGCTGGCCAA GCTGAATGCT 
ATGAATATCA GTGAGACGCG TTATAAGGAA TCCTGGAGCA AACTTCGGGA CGGTGGCTAT 
AAACTGAGGT TGGATGCCAT TCCGTTCCAG GCAGCAAAGG CTTCTGGCGA AATCATAAGT 
GATTACAAAT ACAAAGAGGC ATTTGAGAAA ATGAAAGGGC AGATGCTTGG CTCCCGGAGC 
TTGGAAGACG ATATCAGCCT TGCACATTCA GTGTATGCGA ACTCGCTGCA GAGCGAAGTG 
AATTACAAGA AAGGCTTTGA GCTCTCAAAG GCACACTTCC ATCTGCCGTT GGACATGGTA 
ACCCTGGTGC ATGCCAAGAA GGCTCAGAGC CTGGCCAGCG ACCAGGACTA CAGACACCCG 
CTCCCCCAGT ACACATCCTT GGCTGAAGAC CTGAGGCTCA GCTGCGCCAA GAAAGCTCAC 
AAGCTGCAGA GTGAGAATTT GTACCGCTCA GACCTGAACT TTATGCGAGG CGTTCCCTGT 
GTCATTCCGG GCACATTAGA GATTGAAGGG AGAAAGAAAG CATCTGAACT CATCAGTGAG 
AGTAAATACC GTCAGCATCC CCAGTCATTC AAGTACACAG CTGTGACAGA CACTCCCAGC 
CTCCTTCATG CAAAACTCAG CAACCAGATT ACCAATGAGC GCCTCTATAA AGCAGCCGGA 
GAGGATGAGA GGCACCGGTA CACAATGACC CTGGGTCTGC CCGAGTTTGT CCGAGCGAAA 
GCGAACGCAG CCAATCTGAG TGACGCAAAA TACAAGGAGT CTTGGCGTAA TCTCCGTGCT 
CAAGGCTACA AGCTGACAAT AGACGCTCTC CCCTTCCAGG CTGCTCGCGT CTCTGGAGAT 
ATAGCCAGTG ATTTTCTCTA CAGACATGAC TTTGTGAAGG AGCGAGGCAG GCTGATCGGG 
GCCCGGAGTG TGAGTGATGA CCCCCGACTC CAGCACTGCT GGCGGGTAGG CCAGTTGCAG 
AGCGAGCTTC AGTACAGGCG GGGAGCGGCG AGCAGCCAAG CCCACTACCA CCTGCCCATG 
GACATGGTGC ACCTGGTCCA TGCCAGGAAG GCCCAGGCCC TAGCCAGCGA CCACGACTAC 
AGGACACGGT GCCATGAGTT CACAGCTCTG CCTGAGGACC TGAAGATGTC CTGGGCCAAG 
AGAGCTCACG CCCTGCAGAG TGAGTTTCGC TACAAGTCAG ACCTGATGGG CATGAAGGGG 
ACAGGATGGC TGACGCTGAG TTCGCCGCAG CTAGAGAGTG CAAAGAAGGC CGGAGAACTC 
ATCAGCGAGA CCAAATACCG TAAGAAACCA GATAGTATCA AGTTCACCAC GGTGGTTGAC 
TCCCCAGACC TGGTTCACGC CAAGAACAGC TACATGCATT GCAATGAGCG CCTGTACAGA 
TTGGGGGATG CGGAATCCCT GCACAGATAT ACGCTGATCC CCGACCACCC CGATTTCACC 
AGAGCCCGCC TCAACGCGCT GCATCTGAGT GATAAAGTCT ACAGAAACTC TTGGGAACAG 
ACCCGGGCTG GTGGCTATGA CTTCCGGCTG GATGCCATCC CATTCCAGAC TGCCCGGGCG 
TCCCGGGAGA TCGCCAGTGA TTTCCGGTAC AAAGAGGCCT TCCTGCGGGA CCGGGGCCTA 
CAGATTGGTT ACCGCAGCAT CCACGACGAC CCCAGGATGA GGCATTTCCT CAGCGTCGGC 
AAACTCCAGA GTGACAACGA GTACAAGAAG GACTTTGCCA AGAGTCGGTC CCAGTTCCAT 
AGCCGCCCGG ACCAGCCCGG CTTCCTCCAG GCCAGGAGGA GCCAGCAGCT GGCCAGCGAC 
GTGTGCTACC GGCAGCCCCT GCCCCAGCCC ACATGCGACC CGGAGCAGCT AGGCCTGAAG 
CACGCACAGA AGGCCCACCA GCTGCAGAGT GACGTCAAGT ACAAATCAGA CTTGAACCTG 
ACCAGAGGCG TTGGCTGGAC ACCCCCCGGC TCCTACAAAG TGGAAATGGC TCGGCGGGCG 
GCAGAACTGG CCAAGGCGAG GGGCCTGGGT CTCCGGGGCG CTTGGGGGGG GCCAGAGGCT 
GTGGAGCCCG CAGAGAATCA GAGGGGGGAC GTGAACCCAG ACGCCACCGA GATTCTGCAC 
GTCAAAAGGA GGAAGGCTCT GCCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_014108946.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964421.1 Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X2, mRNA.

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CloneID	OCe43945
Clone ID Related Accession (Same CDS sequence)	XM_014108946.1 , XM_014108946.2
Accession Version	XM_014108946.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	nebulin-related-anchoring protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108946.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGCAGGA GTATACAGAA 
GACTATGAAC AACAGAGGGG CAAAGGGAGC TTTCCAGCCA TGATCACACC TGCCTATCAA 
AGAGCCAAGA AAGCTAACCA GCTGGCCAGC CAAGTGGAAT ACAAGAGAGG GCATGACGAG 
CGCATCTCCA GGTTTACCAC GGTGGCGGAT ACCCCTGAGC TGCTACGGGC CCAGGCAGGG 
GGACAGCTTC AAAGTGATGT GAGATACACA GAAGACTATG AACAACAGAG AGGAAAAGGC 
AGTTTCCCTG CTATGATCAC GCCTGCTTAT CAGATAGCCA AGAGAGCCAA TGAGCTGGCT 
AGCGATGTGA GGTACCATCA ACAATATCAA AGAGAAATGA AGGGAATGGC TAGTCCAGCT 
GCTGGAGCTG CAGGCACAAG GGAATGCATG GACCGATATG GCCAGGGTTA CTTGGAGGAA 
TATCAGGAGC ACAGAGGAAA AGGCAGCTTC CCCGCCATGA TCACCCCAGC TTATCAGAAT 
GCCAAGAAAG CCAACGAACT CGCTAGTGAC ATAAAATACA GGCAGGACTT CAATAAGATG 
AAAGGCCCTG CACATTATCA CTCCCTCCCA GCCCAGGACA ACTTGGTTCT CAAGCGGGCC 
CAGAGCGTGA ACAAGCTTGT GAGCGAGGTG GAATATAAGA AGGACCTGGA AAGTAGTAAA 
GGTCACAGTA TCAACTACTG TGAGACGCCT CAATTCAGGA ACGTGAGCAA GATCTCAAAA 
TTCACCAGTG ATAATAAATA TAAAGAAAAT TACCAGAACC ACATGAGAGG CCACTATGAA 
GGAGTTGGTA TGGACAGACG GACTCTCCAT GCTATGAAGG TTGGAAGCTT GGCAAGCAAT 
ATTGCCTACA AGGCTGATTA TAAACACGAT GTTGTGGACT ACAACTACCC AGCCACTCTT 
ACTCCCTCTT ACCAGACAAC AATGAAACTT GTCCCCTTGA AAGATGTCAA CTACAGGCAA 
AGTATCGACA AGTTGAAGTA CAGCTCTGTG ACCAACACCC CTCAGATTAT CCAAGCCAAA 
ATCAATGCCC AACAACTGAG TCATGTGAAT TATCGTGCTG ACTATGAGAA AAATAAGTTG 
AACTACACCT TGCCACAGGA TGTACCTCAG CTGGTGAAGG CCAAAACCAA TGCTGAACTA 
TTTAGTGAGG TTAAGTATAA AGAAGGCTGG GAGAAGGCCA AGGGGAAAGG ATTTGAGATG 
AAGCTGGATG CTATGTCTTT GCTGGCCGCC AAAGCTTCTG GAGAGCTTGC TAGCAATATT 
AAATATAAAG AAGAGTATGA AAAAACGAAA GGCAAAGTCA TGGGCACTAC AGACTCGAGG 
CTTCTGCACT CTCTGCAGGT GGCCAAGATG AGCAGTGAGG TTGAATACAA GAAAGGCTTT 
GAAGAGAGTA AGACCCACTT TCACCTACCC ATGGATATGG TGCACATCAA GCATGCTAAG 
AAGGCCCAAG CTCTGGCCAG TGACCTGGAC TACAGGAAAA AGCTGCATGA ATACACTGTA 
CTGCCTGAAG ATATGAAGAC TCAGTGGGCC AAGAAAGCCT ATGGGCTCCA GAGTGAGCTG 
CAGTACAAGG CCGACCTAGC ATGGATGAAA GGAGTCGGGT GGCTGACGGA GGGGAGTCTC 
AATTTGGAAC AGGCCAAGAA GGCTGGCCAG CTGGCCAGCG AGAAAAACTA CAGGCAGAGG 
GTGGATGAGC TGAAGTTCAC CAGTGTGACC GACAGCTCCC AGATGGAGCA CGCCAAGAAG 
AGCCAGGAGC TGCAGAGCGG GGTGGCCTAC AAGGCAGGAA ATGAGCAGTC TGTCCATCAG 
TACAGCATCA GCAAAGATGA ACCTCTCTTC TTACAGGCCC GAGTCAATGC CGCCAATCTC 
AGTGAGAAAC TGTATAAGAG CAGCTGGGAA AACCAGAAGG CAAAAGGTTT TGAGCTGCGT 
CTGGACTCCT TGGGCTTCCT GGCAGCCAAA GCCAACAGGG ACCTGGCGAG CGAGGTGAAG 
TACAAGGAAG ATTACGAGAG GTCCCGAGGT AAGCTCATTG GAGCGAAGGA TGCCCAGGGG 
GATTCACAAA TGAGCCACTC GCTGCAAATG TCCAAGCTGC AGAGTGAACT CGAGTACAAG 
AAAGGGTTCG AGGACACCAG ATCCCAGTGC CACGTCTCCC TGGACATGAT CCACCTTGTG 
CATGCCCGCA AGGCTCAGCA TTTAGCCACA GACATAGGCT ACAAGACGGC ATCTCATCAG 
TTCACCGCTT TGCCCACCGA CATGAAGGTG GAATGGGCCA AGAAGGCATA CGGTTTACAG 
AGTGATAACC AATACAGGGC AGATGTGAAG TGGATGAAAG GCACGGGCTG GGTCGCCACC 
GGGTCATTAA ATGTGGAGCA GGCGAAGAAG GCAGGAGAAC TCATTAGCGA GAAGAAGTAC 
CGTCAGCATC CAGATGCTTT GAAGTTTACC AGTATTAAAG ACACTCCGGA GATGGTCCAG 
GCCAGAATTA GTTATACCCA AGCAGTGGAT AGGTTATACA GGGAACAAGG AGAAAATATA 
AAGCATCATT ACACTCAGAC AGCCGACCTC CCTGAAGTCC TGCTGGCCAA GCTGAATGCT 
ATGAATATCA GTGAGACGCG TTATAAGGAA TCCTGGAGCA AACTTCGGGA CGGTGGCTAT 
AAACTGAGGT TGGATGCCAT TCCGTTCCAG GCAGCAAAGG CTTCTGGCGA AATCATAAGT 
GATTACAAAT ACAAAGAGGC ATTTGAGAAA ATGAAAGGGC AGATGCTTGG CTCCCGGAGC 
TTGGAAGACG ATATCAGCCT TGCACATTCA GTGTATGCGA ACTCGCTGCA GAGCGAAGTG 
AATTACAAGA AAGGCTTTGA GCTCTCAAAG GCACACTTCC ATCTGCCGTT GGACATGGTA 
ACCCTGGTGC ATGCCAAGAA GGCTCAGAGC CTGGCCAGCG ACCAGGACTA CAGACACCCG 
CTCCCCCAGT ACACATCCTT GGCTGAAGAC CTGAGGCTCA GCTGCGCCAA GAAAGCTCAC 
AAGCTGCAGA GTGAGAATTT GTACCGCTCA GACCTGAACT TTATGCGAGG CGTTCCCTGT 
GTCATTCCGG GCACATTAGA GATTGAAGGG AGAAAGAAAG CATCTGAACT CATCAGTGAG 
AGTAAATACC GTCAGCATCC CCAGTCATTC AAGTACACAG CTGTGACAGA CACTCCCAGC 
CTCCTTCATG CAAAACTCAG CAACCAGATT ACCAATGAGC GCCTCTATAA AGCAGCCGGA 
GAGGATGAGA GGCACCGGTA CACAATGACC CTGGGTCTGC CCGAGTTTGT CCGAGCGAAA 
GCGAACGCAG CCAATCTGAG TGACGCAAAA TACAAGGAGT CTTGGCGTAA TCTCCGTGCT 
CAAGGCTACA AGCTGACAAT AGACGCTCTC CCCTTCCAGG CTGCTCGCGT CTCTGGAGAT 
ATAGCCAGTG ATTTTCTCTA CAGACATGAC TTTGTGAAGG AGCGAGGCAG GCTGATCGGG 
GCCCGGAGTG TGAGTGATGA CCCCCGACTC CAGCACTGCT GGCGGGTAGG CCAGTTGCAG 
AGCGAGCTTC AGTACAGGCG GGGAGCGGCG AGCAGCCAAG CCCACTACCA CCTGCCCATG 
GACATGGTGC ACCTGGTCCA TGCCAGGAAG GCCCAGGCCC TAGCCAGCGA CCACGACTAC 
AGGACACGGT GCCATGAGTT CACAGCTCTG CCTGAGGACC TGAAGATGTC CTGGGCCAAG 
AGAGCTCACG CCCTGCAGAG TGAGTTTCGC TACAAGTCAG ACCTGATGGG CATGAAGGGG 
ACAGGATGGC TGACGCTGAG TTCGCCGCAG CTAGAGAGTG CAAAGAAGGC CGGAGAACTC 
ATCAGCGAGA CCAAATACCG TAAGAAACCA GATAGTATCA AGTTCACCAC GGTGGTTGAC 
TCCCCAGACC TGGTTCACGC CAAGAACAGC TACATGCATT GCAATGAGCG CCTGTACAGA 
TTGGGGGATG CGGAATCCCT GCACAGATAT ACGCTGATCC CCGACCACCC CGATTTCACC 
AGAGCCCGCC TCAACGCGCT GCATCTGAGT GATAAAGTCT ACAGAAACTC TTGGGAACAG 
ACCCGGGCTG GTGGCTATGA CTTCCGGCTG GATGCCATCC CATTCCAGAC TGCCCGGGCG 
TCCCGGGAGA TCGCCAGTGA TTTCCGGTAC AAAGAGGCCT TCCTGCGGGA CCGGGGCCTA 
CAGATTGGTT ACCGCAGCAT CCACGACGAC CCCAGGATGA GGCATTTCCT CAGCGTCGGC 
AAACTCCAGA GTGACAACGA GTACAAGAAG GACTTTGCCA AGAGTCGGTC CCAGTTCCAT 
AGCCGCCCGG ACCAGCCCGG CTTCCTCCAG GCCAGGAGGA GCCAGCAGCT GGCCAGCGAC 
GTGTGCTACC GGCAGCCCCT GCCCCAGCCC ACATGCGACC CGGAGCAGCT AGGCCTGAAG 
CACGCACAGA AGGCCCACCA GCTGCAGAGT GACGTCAAGT ACAAATCAGA CTTGAACCTG 
ACCAGAGGCG TTGGCTGGAC ACCCCCCGGC TCCTACAAAG TGGAAATGGC TCGGCGGGCG 
GCAGAACTGG CCAAGGCGAG GGGCCTGGGT CTCCGGGGCG CTTGGGGGGG GCCAGAGGCT 
GTGGAGCCCG CAGAGAATCA GAGGGGGGAC GTGAACCCAG ACGCCACCGA GATTCTGCAC 
GTCAAAAGGA GGAAGGCTCT GCCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964421.1
CDS	224..5413
Translation	MNVQACSRCGYGVYPAEKISCLDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRKSPETIHGIDEQDEGERFKSVFHWDMKSRDGTAAPNRQPLMNERAYWNGYGEGNAWCPGAMPDPEIVRMVETRKSLGEQEYTEDYEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKRGHDERISRFTTVADTPELLRAQAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMKGMASPAAGAAGTRECMDRYGQGYLEEYQEHRGKGSFPAMITPAYQNAKKANELASDIKYRQDFNKMKGPAHYHSLPAQDNLVLKRAQSVNKLVSEVEYKKDLESSKGHSINYCETPQFRNVSKISKFTSDNKYKENYQNHMRGHYEGVGMDRRTLHAMKVGSLASNIAYKADYKHDVVDYNYPATLTPSYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIIQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAELFSEVKYKEGWEKAKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKVMGTTDSRLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVHIKHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLASEKNYRQRVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYSISKDEPLFLQARVNAANLSEKLYKSSWENQKAKGFELRLDSLGFLAAKANRDLASEVKYKEDYERSRGKLIGAKDAQGDSQMSHSLQMSKLQSELEYKKGFEDTRSQCHVSLDMIHLVHARKAQHLATDIGYKTASHQFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTADLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASGEIISDYKYKEAFEKMKGQMLGSRSLEDDISLAHSVYANSLQSEVNYKKGFELSKAHFHLPLDMVTLVHAKKAQSLASDQDYRHPLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVPCVIPGTLEIEGRKKASELISESKYRQHPQSFKYTAVTDTPSLLHAKLSNQITNERLYKAAGEDERHRYTMTLGLPEFVRAKANAANLSDAKYKESWRNLRAQGYKLTIDALPFQAARVSGDIASDFLYRHDFVKERGRLIGARSVSDDPRLQHCWRVGQLQSELQYRRGAASSQAHYHLPMDMVHLVHARKAQALASDHDYRTRCHEFTALPEDLKMSWAKRAHALQSEFRYKSDLMGMKGTGWLTLSSPQLESAKKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERLYRLGDAESLHRYTLIPDHPDFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGYRSIHDDPRMRHFLSVGKLQSDNEYKKDFAKSRSQFHSRPDQPGFLQARRSQQLASDVCYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELAKARGLGLRGAWGGPEAVEPAENQRGDVNPDATEILHVKRRKALPL

Target ORF information:

RefSeq Version	XM_014108946.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108946.2

ORF Insert Sequence:

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGCAGGA GTATACAGAA 
GACTATGAAC AACAGAGGGG CAAAGGGAGC TTTCCAGCCA TGATCACACC TGCCTATCAA 
AGAGCCAAGA AAGCTAACCA GCTGGCCAGC CAAGTGGAAT ACAAGAGAGG GCATGACGAG 
CGCATCTCCA GGTTTACCAC GGTGGCGGAT ACCCCTGAGC TGCTACGGGC CCAGGCAGGG 
GGACAGCTTC AAAGTGATGT GAGATACACA GAAGACTATG AACAACAGAG AGGAAAAGGC 
AGTTTCCCTG CTATGATCAC GCCTGCTTAT CAGATAGCCA AGAGAGCCAA TGAGCTGGCT 
AGCGATGTGA GGTACCATCA ACAATATCAA AGAGAAATGA AGGGAATGGC TAGTCCAGCT 
GCTGGAGCTG CAGGCACAAG GGAATGCATG GACCGATATG GCCAGGGTTA CTTGGAGGAA 
TATCAGGAGC ACAGAGGAAA AGGCAGCTTC CCCGCCATGA TCACCCCAGC TTATCAGAAT 
GCCAAGAAAG CCAACGAACT CGCTAGTGAC ATAAAATACA GGCAGGACTT CAATAAGATG 
AAAGGCCCTG CACATTATCA CTCCCTCCCA GCCCAGGACA ACTTGGTTCT CAAGCGGGCC 
CAGAGCGTGA ACAAGCTTGT GAGCGAGGTG GAATATAAGA AGGACCTGGA AAGTAGTAAA 
GGTCACAGTA TCAACTACTG TGAGACGCCT CAATTCAGGA ACGTGAGCAA GATCTCAAAA 
TTCACCAGTG ATAATAAATA TAAAGAAAAT TACCAGAACC ACATGAGAGG CCACTATGAA 
GGAGTTGGTA TGGACAGACG GACTCTCCAT GCTATGAAGG TTGGAAGCTT GGCAAGCAAT 
ATTGCCTACA AGGCTGATTA TAAACACGAT GTTGTGGACT ACAACTACCC AGCCACTCTT 
ACTCCCTCTT ACCAGACAAC AATGAAACTT GTCCCCTTGA AAGATGTCAA CTACAGGCAA 
AGTATCGACA AGTTGAAGTA CAGCTCTGTG ACCAACACCC CTCAGATTAT CCAAGCCAAA 
ATCAATGCCC AACAACTGAG TCATGTGAAT TATCGTGCTG ACTATGAGAA AAATAAGTTG 
AACTACACCT TGCCACAGGA TGTACCTCAG CTGGTGAAGG CCAAAACCAA TGCTGAACTA 
TTTAGTGAGG TTAAGTATAA AGAAGGCTGG GAGAAGGCCA AGGGGAAAGG ATTTGAGATG 
AAGCTGGATG CTATGTCTTT GCTGGCCGCC AAAGCTTCTG GAGAGCTTGC TAGCAATATT 
AAATATAAAG AAGAGTATGA AAAAACGAAA GGCAAAGTCA TGGGCACTAC AGACTCGAGG 
CTTCTGCACT CTCTGCAGGT GGCCAAGATG AGCAGTGAGG TTGAATACAA GAAAGGCTTT 
GAAGAGAGTA AGACCCACTT TCACCTACCC ATGGATATGG TGCACATCAA GCATGCTAAG 
AAGGCCCAAG CTCTGGCCAG TGACCTGGAC TACAGGAAAA AGCTGCATGA ATACACTGTA 
CTGCCTGAAG ATATGAAGAC TCAGTGGGCC AAGAAAGCCT ATGGGCTCCA GAGTGAGCTG 
CAGTACAAGG CCGACCTAGC ATGGATGAAA GGAGTCGGGT GGCTGACGGA GGGGAGTCTC 
AATTTGGAAC AGGCCAAGAA GGCTGGCCAG CTGGCCAGCG AGAAAAACTA CAGGCAGAGG 
GTGGATGAGC TGAAGTTCAC CAGTGTGACC GACAGCTCCC AGATGGAGCA CGCCAAGAAG 
AGCCAGGAGC TGCAGAGCGG GGTGGCCTAC AAGGCAGGAA ATGAGCAGTC TGTCCATCAG 
TACAGCATCA GCAAAGATGA ACCTCTCTTC TTACAGGCCC GAGTCAATGC CGCCAATCTC 
AGTGAGAAAC TGTATAAGAG CAGCTGGGAA AACCAGAAGG CAAAAGGTTT TGAGCTGCGT 
CTGGACTCCT TGGGCTTCCT GGCAGCCAAA GCCAACAGGG ACCTGGCGAG CGAGGTGAAG 
TACAAGGAAG ATTACGAGAG GTCCCGAGGT AAGCTCATTG GAGCGAAGGA TGCCCAGGGG 
GATTCACAAA TGAGCCACTC GCTGCAAATG TCCAAGCTGC AGAGTGAACT CGAGTACAAG 
AAAGGGTTCG AGGACACCAG ATCCCAGTGC CACGTCTCCC TGGACATGAT CCACCTTGTG 
CATGCCCGCA AGGCTCAGCA TTTAGCCACA GACATAGGCT ACAAGACGGC ATCTCATCAG 
TTCACCGCTT TGCCCACCGA CATGAAGGTG GAATGGGCCA AGAAGGCATA CGGTTTACAG 
AGTGATAACC AATACAGGGC AGATGTGAAG TGGATGAAAG GCACGGGCTG GGTCGCCACC 
GGGTCATTAA ATGTGGAGCA GGCGAAGAAG GCAGGAGAAC TCATTAGCGA GAAGAAGTAC 
CGTCAGCATC CAGATGCTTT GAAGTTTACC AGTATTAAAG ACACTCCGGA GATGGTCCAG 
GCCAGAATTA GTTATACCCA AGCAGTGGAT AGGTTATACA GGGAACAAGG AGAAAATATA 
AAGCATCATT ACACTCAGAC AGCCGACCTC CCTGAAGTCC TGCTGGCCAA GCTGAATGCT 
ATGAATATCA GTGAGACGCG TTATAAGGAA TCCTGGAGCA AACTTCGGGA CGGTGGCTAT 
AAACTGAGGT TGGATGCCAT TCCGTTCCAG GCAGCAAAGG CTTCTGGCGA AATCATAAGT 
GATTACAAAT ACAAAGAGGC ATTTGAGAAA ATGAAAGGGC AGATGCTTGG CTCCCGGAGC 
TTGGAAGACG ATATCAGCCT TGCACATTCA GTGTATGCGA ACTCGCTGCA GAGCGAAGTG 
AATTACAAGA AAGGCTTTGA GCTCTCAAAG GCACACTTCC ATCTGCCGTT GGACATGGTA 
ACCCTGGTGC ATGCCAAGAA GGCTCAGAGC CTGGCCAGCG ACCAGGACTA CAGACACCCG 
CTCCCCCAGT ACACATCCTT GGCTGAAGAC CTGAGGCTCA GCTGCGCCAA GAAAGCTCAC 
AAGCTGCAGA GTGAGAATTT GTACCGCTCA GACCTGAACT TTATGCGAGG CGTTCCCTGT 
GTCATTCCGG GCACATTAGA GATTGAAGGG AGAAAGAAAG CATCTGAACT CATCAGTGAG 
AGTAAATACC GTCAGCATCC CCAGTCATTC AAGTACACAG CTGTGACAGA CACTCCCAGC 
CTCCTTCATG CAAAACTCAG CAACCAGATT ACCAATGAGC GCCTCTATAA AGCAGCCGGA 
GAGGATGAGA GGCACCGGTA CACAATGACC CTGGGTCTGC CCGAGTTTGT CCGAGCGAAA 
GCGAACGCAG CCAATCTGAG TGACGCAAAA TACAAGGAGT CTTGGCGTAA TCTCCGTGCT 
CAAGGCTACA AGCTGACAAT AGACGCTCTC CCCTTCCAGG CTGCTCGCGT CTCTGGAGAT 
ATAGCCAGTG ATTTTCTCTA CAGACATGAC TTTGTGAAGG AGCGAGGCAG GCTGATCGGG 
GCCCGGAGTG TGAGTGATGA CCCCCGACTC CAGCACTGCT GGCGGGTAGG CCAGTTGCAG 
AGCGAGCTTC AGTACAGGCG GGGAGCGGCG AGCAGCCAAG CCCACTACCA CCTGCCCATG 
GACATGGTGC ACCTGGTCCA TGCCAGGAAG GCCCAGGCCC TAGCCAGCGA CCACGACTAC 
AGGACACGGT GCCATGAGTT CACAGCTCTG CCTGAGGACC TGAAGATGTC CTGGGCCAAG 
AGAGCTCACG CCCTGCAGAG TGAGTTTCGC TACAAGTCAG ACCTGATGGG CATGAAGGGG 
ACAGGATGGC TGACGCTGAG TTCGCCGCAG CTAGAGAGTG CAAAGAAGGC CGGAGAACTC 
ATCAGCGAGA CCAAATACCG TAAGAAACCA GATAGTATCA AGTTCACCAC GGTGGTTGAC 
TCCCCAGACC TGGTTCACGC CAAGAACAGC TACATGCATT GCAATGAGCG CCTGTACAGA 
TTGGGGGATG CGGAATCCCT GCACAGATAT ACGCTGATCC CCGACCACCC CGATTTCACC 
AGAGCCCGCC TCAACGCGCT GCATCTGAGT GATAAAGTCT ACAGAAACTC TTGGGAACAG 
ACCCGGGCTG GTGGCTATGA CTTCCGGCTG GATGCCATCC CATTCCAGAC TGCCCGGGCG 
TCCCGGGAGA TCGCCAGTGA TTTCCGGTAC AAAGAGGCCT TCCTGCGGGA CCGGGGCCTA 
CAGATTGGTT ACCGCAGCAT CCACGACGAC CCCAGGATGA GGCATTTCCT CAGCGTCGGC 
AAACTCCAGA GTGACAACGA GTACAAGAAG GACTTTGCCA AGAGTCGGTC CCAGTTCCAT 
AGCCGCCCGG ACCAGCCCGG CTTCCTCCAG GCCAGGAGGA GCCAGCAGCT GGCCAGCGAC 
GTGTGCTACC GGCAGCCCCT GCCCCAGCCC ACATGCGACC CGGAGCAGCT AGGCCTGAAG 
CACGCACAGA AGGCCCACCA GCTGCAGAGT GACGTCAAGT ACAAATCAGA CTTGAACCTG 
ACCAGAGGCG TTGGCTGGAC ACCCCCCGGC TCCTACAAAG TGGAAATGGC TCGGCGGGCG 
GCAGAACTGG CCAAGGCGAG GGGCCTGGGT CTCCGGGGCG CTTGGGGGGG GCCAGAGGCT 
GTGGAGCCCG CAGAGAATCA GAGGGGGGAC GTGAACCCAG ACGCCACCGA GATTCTGCAC 
GTCAAAAGGA GGAAGGCTCT GCCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_014108947.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964422.1 Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe43946
Clone ID Related Accession (Same CDS sequence)	XM_014108947.1 , XM_014108947.2
Accession Version	XM_014108947.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	nebulin-related-anchoring protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGGAGTA TACAGAAGAC 
TATGAACAAC AGAGGGGCAA AGGGAGCTTT CCAGCCATGA TCACACCTGC CTATCAAAGA 
GCCAAGAAAG CTAACCAGCT GGCCAGCCAA GTGGAATACA AGAGAGGGCA TGACGAGCGC 
ATCTCCAGGT TTACCACGGT GGCGGATACC CCTGAGCTGC TACGGGCCCA GGCAGGGGGA 
CAGCTTCAAA GTGATGTGAG ATACACAGAA GACTATGAAC AACAGAGAGG AAAAGGCAGT 
TTCCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAGA GAGCCAATGA GCTGGCTAGC 
GATGTGAGGT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTAG TCCAGCTGCT 
GGAGCTGCAG GCACAAGGGA ATGCATGGAC CGATATGGCC AGCAGGGTTA CTTGGAGGAA 
TATCAGGAGC ACAGAGGAAA AGGCAGCTTC CCCGCCATGA TCACCCCAGC TTATCAGAAT 
GCCAAGAAAG CCAACGAACT CGCTAGTGAC ATAAAATACA GGCAGGACTT CAATAAGATG 
AAAGGCCCTG CACATTATCA CTCCCTCCCA GCCCAGGACA ACTTGGTTCT CAAGCGGGCC 
CAGAGCGTGA ACAAGCTTGT GAGCGAGGTG GAATATAAGA AGGACCTGGA AAGTAGTAAA 
GGTCACAGTA TCAACTACTG TGAGACGCCT CAATTCAGGA ACGTGAGCAA GATCTCAAAA 
TTCACCAGTG ATAATAAATA TAAAGAAAAT TACCAGAACC ACATGAGAGG CCACTATGAA 
GGAGTTGGTA TGGACAGACG GACTCTCCAT GCTATGAAGG TTGGAAGCTT GGCAAGCAAT 
ATTGCCTACA AGGCTGATTA TAAACACGAT GTTGTGGACT ACAACTACCC AGCCACTCTT 
ACTCCCTCTT ACCAGACAAC AATGAAACTT GTCCCCTTGA AAGATGTCAA CTACAGGCAA 
AGTATCGACA AGTTGAAGTA CAGCTCTGTG ACCAACACCC CTCAGATTAT CCAAGCCAAA 
ATCAATGCCC AACAACTGAG TCATGTGAAT TATCGTGCTG ACTATGAGAA AAATAAGTTG 
AACTACACCT TGCCACAGGA TGTACCTCAG CTGGTGAAGG CCAAAACCAA TGCTGAACTA 
TTTAGTGAGG TTAAGTATAA AGAAGGCTGG GAGAAGGCCA AGGGGAAAGG ATTTGAGATG 
AAGCTGGATG CTATGTCTTT GCTGGCCGCC AAAGCTTCTG GAGAGCTTGC TAGCAATATT 
AAATATAAAG AAGAGTATGA AAAAACGAAA GGCAAAGTCA TGGGCACTAC AGACTCGAGG 
CTTCTGCACT CTCTGCAGGT GGCCAAGATG AGCAGTGAGG TTGAATACAA GAAAGGCTTT 
GAAGAGAGTA AGACCCACTT TCACCTACCC ATGGATATGG TGCACATCAA GCATGCTAAG 
AAGGCCCAAG CTCTGGCCAG TGACCTGGAC TACAGGAAAA AGCTGCATGA ATACACTGTA 
CTGCCTGAAG ATATGAAGAC TCAGTGGGCC AAGAAAGCCT ATGGGCTCCA GAGTGAGCTG 
CAGTACAAGG CCGACCTAGC ATGGATGAAA GGAGTCGGGT GGCTGACGGA GGGGAGTCTC 
AATTTGGAAC AGGCCAAGAA GGCTGGCCAG CTGGCCAGCG AGAAAAACTA CAGGCAGAGG 
GTGGATGAGC TGAAGTTCAC CAGTGTGACC GACAGCTCCC AGATGGAGCA CGCCAAGAAG 
AGCCAGGAGC TGCAGAGCGG GGTGGCCTAC AAGGCAGGAA ATGAGCAGTC TGTCCATCAG 
TACAGCATCA GCAAAGATGA ACCTCTCTTC TTACAGGCCC GAGTCAATGC CGCCAATCTC 
AGTGAGAAAC TGTATAAGAG CAGCTGGGAA AACCAGAAGG CAAAAGGTTT TGAGCTGCGT 
CTGGACTCCT TGGGCTTCCT GGCAGCCAAA GCCAACAGGG ACCTGGCGAG CGAGGTGAAG 
TACAAGGAAG ATTACGAGAG GTCCCGAGGT AAGCTCATTG GAGCGAAGGA TGCCCAGGGG 
GATTCACAAA TGAGCCACTC GCTGCAAATG TCCAAGCTGC AGAGTGAACT CGAGTACAAG 
AAAGGGTTCG AGGACACCAG ATCCCAGTGC CACGTCTCCC TGGACATGAT CCACCTTGTG 
CATGCCCGCA AGGCTCAGCA TTTAGCCACA GACATAGGCT ACAAGACGGC ATCTCATCAG 
TTCACCGCTT TGCCCACCGA CATGAAGGTG GAATGGGCCA AGAAGGCATA CGGTTTACAG 
AGTGATAACC AATACAGGGC AGATGTGAAG TGGATGAAAG GCACGGGCTG GGTCGCCACC 
GGGTCATTAA ATGTGGAGCA GGCGAAGAAG GCAGGAGAAC TCATTAGCGA GAAGAAGTAC 
CGTCAGCATC CAGATGCTTT GAAGTTTACC AGTATTAAAG ACACTCCGGA GATGGTCCAG 
GCCAGAATTA GTTATACCCA AGCAGTGGAT AGGTTATACA GGGAACAAGG AGAAAATATA 
AAGCATCATT ACACTCAGAC AGCCGACCTC CCTGAAGTCC TGCTGGCCAA GCTGAATGCT 
ATGAATATCA GTGAGACGCG TTATAAGGAA TCCTGGAGCA AACTTCGGGA CGGTGGCTAT 
AAACTGAGGT TGGATGCCAT TCCGTTCCAG GCAGCAAAGG CTTCTGGCGA AATCATAAGT 
GATTACAAAT ACAAAGAGGC ATTTGAGAAA ATGAAAGGGC AGATGCTTGG CTCCCGGAGC 
TTGGAAGACG ATATCAGCCT TGCACATTCA GTGTATGCGA ACTCGCTGCA GAGCGAAGTG 
AATTACAAGA AAGGCTTTGA GCTCTCAAAG GCACACTTCC ATCTGCCGTT GGACATGGTA 
ACCCTGGTGC ATGCCAAGAA GGCTCAGAGC CTGGCCAGCG ACCAGGACTA CAGACACCCG 
CTCCCCCAGT ACACATCCTT GGCTGAAGAC CTGAGGCTCA GCTGCGCCAA GAAAGCTCAC 
AAGCTGCAGA GTGAGAATTT GTACCGCTCA GACCTGAACT TTATGCGAGG CGTTCCCTGT 
GTCATTCCGG GCACATTAGA GATTGAAGGG AGAAAGAAAG CATCTGAACT CATCAGTGAG 
AGTAAATACC GTCAGCATCC CCAGTCATTC AAGTACACAG CTGTGACAGA CACTCCCAGC 
CTCCTTCATG CAAAACTCAG CAACCAGATT ACCAATGAGC GCCTCTATAA AGCAGCCGGA 
GAGGATGAGA GGCACCGGTA CACAATGACC CTGGGTCTGC CCGAGTTTGT CCGAGCGAAA 
GCGAACGCAG CCAATCTGAG TGACGCAAAA TACAAGGAGT CTTGGCGTAA TCTCCGTGCT 
CAAGGCTACA AGCTGACAAT AGACGCTCTC CCCTTCCAGG CTGCTCGCGT CTCTGGAGAT 
ATAGCCAGTG ATTTTCTCTA CAGACATGAC TTTGTGAAGG AGCGAGGCAG GCTGATCGGG 
GCCCGGAGTG TGAGTGATGA CCCCCGACTC CAGCACTGCT GGCGGGTAGG CCAGTTGCAG 
AGCGAGCTTC AGTACAGGCG GGGAGCGGCG AGCAGCCAAG CCCACTACCA CCTGCCCATG 
GACATGGTGC ACCTGGTCCA TGCCAGGAAG GCCCAGGCCC TAGCCAGCGA CCACGACTAC 
AGGACACGGT GCCATGAGTT CACAGCTCTG CCTGAGGACC TGAAGATGTC CTGGGCCAAG 
AGAGCTCACG CCCTGCAGAG TGAGTTTCGC TACAAGTCAG ACCTGATGGG CATGAAGGGG 
ACAGGATGGC TGACGCTGAG TTCGCCGCAG CTAGAGAGTG CAAAGAAGGC CGGAGAACTC 
ATCAGCGAGA CCAAATACCG TAAGAAACCA GATAGTATCA AGTTCACCAC GGTGGTTGAC 
TCCCCAGACC TGGTTCACGC CAAGAACAGC TACATGCATT GCAATGAGCG CCTGTACAGA 
TTGGGGGATG CGGAATCCCT GCACAGATAT ACGCTGATCC CCGACCACCC CGATTTCACC 
AGAGCCCGCC TCAACGCGCT GCATCTGAGT GATAAAGTCT ACAGAAACTC TTGGGAACAG 
ACCCGGGCTG GTGGCTATGA CTTCCGGCTG GATGCCATCC CATTCCAGAC TGCCCGGGCG 
TCCCGGGAGA TCGCCAGTGA TTTCCGGTAC AAAGAGGCCT TCCTGCGGGA CCGGGGCCTA 
CAGATTGGTT ACCGCAGCAT CCACGACGAC CCCAGGATGA GGCATTTCCT CAGCGTCGGC 
AAACTCCAGA GTGACAACGA GTACAAGAAG GACTTTGCCA AGAGTCGGTC CCAGTTCCAT 
AGCCGCCCGG ACCAGCCCGG CTTCCTCCAG GCCAGGAGGA GCCAGCAGCT GGCCAGCGAC 
GTGTGCTACC GGCAGCCCCT GCCCCAGCCC ACATGCGACC CGGAGCAGCT AGGCCTGAAG 
CACGCACAGA AGGCCCACCA GCTGCAGAGT GACGTCAAGT ACAAATCAGA CTTGAACCTG 
ACCAGAGGCG TTGGCTGGAC ACCCCCCGGC TCCTACAAAG TGGAAATGGC TCGGCGGGCG 
GCAGAACTGG CCAAGGCGAG GGGCCTGGGT CTCCGGGGCG CTTGGGGGGG GCCAGAGGCT 
GTGGAGCCCG CAGAGAATCA GAGGGGGGAC GTGAACCCAG ACGCCACCGA GATTCTGCAC 
GTCAAAAGGA GGAAGGCTCT GCCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964422.1
CDS	203..5392
Translation	MNVQACSRCGYGVYPAEKISCLDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRKSPETIHGIDEQDEGERFKSVFHWDMKSRDGTAAPNRQPLMNERAYWNGYGEGNAWCPGAMPDPEIVRMVETRKSLGEEYTEDYEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKRGHDERISRFTTVADTPELLRAQAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMKGMASPAAGAAGTRECMDRYGQQGYLEEYQEHRGKGSFPAMITPAYQNAKKANELASDIKYRQDFNKMKGPAHYHSLPAQDNLVLKRAQSVNKLVSEVEYKKDLESSKGHSINYCETPQFRNVSKISKFTSDNKYKENYQNHMRGHYEGVGMDRRTLHAMKVGSLASNIAYKADYKHDVVDYNYPATLTPSYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIIQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAELFSEVKYKEGWEKAKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKVMGTTDSRLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVHIKHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLASEKNYRQRVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYSISKDEPLFLQARVNAANLSEKLYKSSWENQKAKGFELRLDSLGFLAAKANRDLASEVKYKEDYERSRGKLIGAKDAQGDSQMSHSLQMSKLQSELEYKKGFEDTRSQCHVSLDMIHLVHARKAQHLATDIGYKTASHQFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTADLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASGEIISDYKYKEAFEKMKGQMLGSRSLEDDISLAHSVYANSLQSEVNYKKGFELSKAHFHLPLDMVTLVHAKKAQSLASDQDYRHPLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVPCVIPGTLEIEGRKKASELISESKYRQHPQSFKYTAVTDTPSLLHAKLSNQITNERLYKAAGEDERHRYTMTLGLPEFVRAKANAANLSDAKYKESWRNLRAQGYKLTIDALPFQAARVSGDIASDFLYRHDFVKERGRLIGARSVSDDPRLQHCWRVGQLQSELQYRRGAASSQAHYHLPMDMVHLVHARKAQALASDHDYRTRCHEFTALPEDLKMSWAKRAHALQSEFRYKSDLMGMKGTGWLTLSSPQLESAKKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERLYRLGDAESLHRYTLIPDHPDFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGYRSIHDDPRMRHFLSVGKLQSDNEYKKDFAKSRSQFHSRPDQPGFLQARRSQQLASDVCYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELAKARGLGLRGAWGGPEAVEPAENQRGDVNPDATEILHVKRRKALPL

Target ORF information:

RefSeq Version	XM_014108947.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108947.1

ORF Insert Sequence:

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGGAGTA TACAGAAGAC 
TATGAACAAC AGAGGGGCAA AGGGAGCTTT CCAGCCATGA TCACACCTGC CTATCAAAGA 
GCCAAGAAAG CTAACCAGCT GGCCAGCCAA GTGGAATACA AGAGAGGGCA TGACGAGCGC 
ATCTCCAGGT TTACCACGGT GGCGGATACC CCTGAGCTGC TACGGGCCCA GGCAGGGGGA 
CAGCTTCAAA GTGATGTGAG ATACACAGAA GACTATGAAC AACAGAGAGG AAAAGGCAGT 
TTCCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAGA GAGCCAATGA GCTGGCTAGC 
GATGTGAGGT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTAG TCCAGCTGCT 
GGAGCTGCAG GCACAAGGGA ATGCATGGAC CGATATGGCC AGCAGGGTTA CTTGGAGGAA 
TATCAGGAGC ACAGAGGAAA AGGCAGCTTC CCCGCCATGA TCACCCCAGC TTATCAGAAT 
GCCAAGAAAG CCAACGAACT CGCTAGTGAC ATAAAATACA GGCAGGACTT CAATAAGATG 
AAAGGCCCTG CACATTATCA CTCCCTCCCA GCCCAGGACA ACTTGGTTCT CAAGCGGGCC 
CAGAGCGTGA ACAAGCTTGT GAGCGAGGTG GAATATAAGA AGGACCTGGA AAGTAGTAAA 
GGTCACAGTA TCAACTACTG TGAGACGCCT CAATTCAGGA ACGTGAGCAA GATCTCAAAA 
TTCACCAGTG ATAATAAATA TAAAGAAAAT TACCAGAACC ACATGAGAGG CCACTATGAA 
GGAGTTGGTA TGGACAGACG GACTCTCCAT GCTATGAAGG TTGGAAGCTT GGCAAGCAAT 
ATTGCCTACA AGGCTGATTA TAAACACGAT GTTGTGGACT ACAACTACCC AGCCACTCTT 
ACTCCCTCTT ACCAGACAAC AATGAAACTT GTCCCCTTGA AAGATGTCAA CTACAGGCAA 
AGTATCGACA AGTTGAAGTA CAGCTCTGTG ACCAACACCC CTCAGATTAT CCAAGCCAAA 
ATCAATGCCC AACAACTGAG TCATGTGAAT TATCGTGCTG ACTATGAGAA AAATAAGTTG 
AACTACACCT TGCCACAGGA TGTACCTCAG CTGGTGAAGG CCAAAACCAA TGCTGAACTA 
TTTAGTGAGG TTAAGTATAA AGAAGGCTGG GAGAAGGCCA AGGGGAAAGG ATTTGAGATG 
AAGCTGGATG CTATGTCTTT GCTGGCCGCC AAAGCTTCTG GAGAGCTTGC TAGCAATATT 
AAATATAAAG AAGAGTATGA AAAAACGAAA GGCAAAGTCA TGGGCACTAC AGACTCGAGG 
CTTCTGCACT CTCTGCAGGT GGCCAAGATG AGCAGTGAGG TTGAATACAA GAAAGGCTTT 
GAAGAGAGTA AGACCCACTT TCACCTACCC ATGGATATGG TGCACATCAA GCATGCTAAG 
AAGGCCCAAG CTCTGGCCAG TGACCTGGAC TACAGGAAAA AGCTGCATGA ATACACTGTA 
CTGCCTGAAG ATATGAAGAC TCAGTGGGCC AAGAAAGCCT ATGGGCTCCA GAGTGAGCTG 
CAGTACAAGG CCGACCTAGC ATGGATGAAA GGAGTCGGGT GGCTGACGGA GGGGAGTCTC 
AATTTGGAAC AGGCCAAGAA GGCTGGCCAG CTGGCCAGCG AGAAAAACTA CAGGCAGAGG 
GTGGATGAGC TGAAGTTCAC CAGTGTGACC GACAGCTCCC AGATGGAGCA CGCCAAGAAG 
AGCCAGGAGC TGCAGAGCGG GGTGGCCTAC AAGGCAGGAA ATGAGCAGTC TGTCCATCAG 
TACAGCATCA GCAAAGATGA ACCTCTCTTC TTACAGGCCC GAGTCAATGC CGCCAATCTC 
AGTGAGAAAC TGTATAAGAG CAGCTGGGAA AACCAGAAGG CAAAAGGTTT TGAGCTGCGT 
CTGGACTCCT TGGGCTTCCT GGCAGCCAAA GCCAACAGGG ACCTGGCGAG CGAGGTGAAG 
TACAAGGAAG ATTACGAGAG GTCCCGAGGT AAGCTCATTG GAGCGAAGGA TGCCCAGGGG 
GATTCACAAA TGAGCCACTC GCTGCAAATG TCCAAGCTGC AGAGTGAACT CGAGTACAAG 
AAAGGGTTCG AGGACACCAG ATCCCAGTGC CACGTCTCCC TGGACATGAT CCACCTTGTG 
CATGCCCGCA AGGCTCAGCA TTTAGCCACA GACATAGGCT ACAAGACGGC ATCTCATCAG 
TTCACCGCTT TGCCCACCGA CATGAAGGTG GAATGGGCCA AGAAGGCATA CGGTTTACAG 
AGTGATAACC AATACAGGGC AGATGTGAAG TGGATGAAAG GCACGGGCTG GGTCGCCACC 
GGGTCATTAA ATGTGGAGCA GGCGAAGAAG GCAGGAGAAC TCATTAGCGA GAAGAAGTAC 
CGTCAGCATC CAGATGCTTT GAAGTTTACC AGTATTAAAG ACACTCCGGA GATGGTCCAG 
GCCAGAATTA GTTATACCCA AGCAGTGGAT AGGTTATACA GGGAACAAGG AGAAAATATA 
AAGCATCATT ACACTCAGAC AGCCGACCTC CCTGAAGTCC TGCTGGCCAA GCTGAATGCT 
ATGAATATCA GTGAGACGCG TTATAAGGAA TCCTGGAGCA AACTTCGGGA CGGTGGCTAT 
AAACTGAGGT TGGATGCCAT TCCGTTCCAG GCAGCAAAGG CTTCTGGCGA AATCATAAGT 
GATTACAAAT ACAAAGAGGC ATTTGAGAAA ATGAAAGGGC AGATGCTTGG CTCCCGGAGC 
TTGGAAGACG ATATCAGCCT TGCACATTCA GTGTATGCGA ACTCGCTGCA GAGCGAAGTG 
AATTACAAGA AAGGCTTTGA GCTCTCAAAG GCACACTTCC ATCTGCCGTT GGACATGGTA 
ACCCTGGTGC ATGCCAAGAA GGCTCAGAGC CTGGCCAGCG ACCAGGACTA CAGACACCCG 
CTCCCCCAGT ACACATCCTT GGCTGAAGAC CTGAGGCTCA GCTGCGCCAA GAAAGCTCAC 
AAGCTGCAGA GTGAGAATTT GTACCGCTCA GACCTGAACT TTATGCGAGG CGTTCCCTGT 
GTCATTCCGG GCACATTAGA GATTGAAGGG AGAAAGAAAG CATCTGAACT CATCAGTGAG 
AGTAAATACC GTCAGCATCC CCAGTCATTC AAGTACACAG CTGTGACAGA CACTCCCAGC 
CTCCTTCATG CAAAACTCAG CAACCAGATT ACCAATGAGC GCCTCTATAA AGCAGCCGGA 
GAGGATGAGA GGCACCGGTA CACAATGACC CTGGGTCTGC CCGAGTTTGT CCGAGCGAAA 
GCGAACGCAG CCAATCTGAG TGACGCAAAA TACAAGGAGT CTTGGCGTAA TCTCCGTGCT 
CAAGGCTACA AGCTGACAAT AGACGCTCTC CCCTTCCAGG CTGCTCGCGT CTCTGGAGAT 
ATAGCCAGTG ATTTTCTCTA CAGACATGAC TTTGTGAAGG AGCGAGGCAG GCTGATCGGG 
GCCCGGAGTG TGAGTGATGA CCCCCGACTC CAGCACTGCT GGCGGGTAGG CCAGTTGCAG 
AGCGAGCTTC AGTACAGGCG GGGAGCGGCG AGCAGCCAAG CCCACTACCA CCTGCCCATG 
GACATGGTGC ACCTGGTCCA TGCCAGGAAG GCCCAGGCCC TAGCCAGCGA CCACGACTAC 
AGGACACGGT GCCATGAGTT CACAGCTCTG CCTGAGGACC TGAAGATGTC CTGGGCCAAG 
AGAGCTCACG CCCTGCAGAG TGAGTTTCGC TACAAGTCAG ACCTGATGGG CATGAAGGGG 
ACAGGATGGC TGACGCTGAG TTCGCCGCAG CTAGAGAGTG CAAAGAAGGC CGGAGAACTC 
ATCAGCGAGA CCAAATACCG TAAGAAACCA GATAGTATCA AGTTCACCAC GGTGGTTGAC 
TCCCCAGACC TGGTTCACGC CAAGAACAGC TACATGCATT GCAATGAGCG CCTGTACAGA 
TTGGGGGATG CGGAATCCCT GCACAGATAT ACGCTGATCC CCGACCACCC CGATTTCACC 
AGAGCCCGCC TCAACGCGCT GCATCTGAGT GATAAAGTCT ACAGAAACTC TTGGGAACAG 
ACCCGGGCTG GTGGCTATGA CTTCCGGCTG GATGCCATCC CATTCCAGAC TGCCCGGGCG 
TCCCGGGAGA TCGCCAGTGA TTTCCGGTAC AAAGAGGCCT TCCTGCGGGA CCGGGGCCTA 
CAGATTGGTT ACCGCAGCAT CCACGACGAC CCCAGGATGA GGCATTTCCT CAGCGTCGGC 
AAACTCCAGA GTGACAACGA GTACAAGAAG GACTTTGCCA AGAGTCGGTC CCAGTTCCAT 
AGCCGCCCGG ACCAGCCCGG CTTCCTCCAG GCCAGGAGGA GCCAGCAGCT GGCCAGCGAC 
GTGTGCTACC GGCAGCCCCT GCCCCAGCCC ACATGCGACC CGGAGCAGCT AGGCCTGAAG 
CACGCACAGA AGGCCCACCA GCTGCAGAGT GACGTCAAGT ACAAATCAGA CTTGAACCTG 
ACCAGAGGCG TTGGCTGGAC ACCCCCCGGC TCCTACAAAG TGGAAATGGC TCGGCGGGCG 
GCAGAACTGG CCAAGGCGAG GGGCCTGGGT CTCCGGGGCG CTTGGGGGGG GCCAGAGGCT 
GTGGAGCCCG CAGAGAATCA GAGGGGGGAC GTGAACCCAG ACGCCACCGA GATTCTGCAC 
GTCAAAAGGA GGAAGGCTCT GCCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_014108947.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964422.1 Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe43946
Clone ID Related Accession (Same CDS sequence)	XM_014108947.1 , XM_014108947.2
Accession Version	XM_014108947.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	nebulin-related-anchoring protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108947.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGGAGTA TACAGAAGAC 
TATGAACAAC AGAGGGGCAA AGGGAGCTTT CCAGCCATGA TCACACCTGC CTATCAAAGA 
GCCAAGAAAG CTAACCAGCT GGCCAGCCAA GTGGAATACA AGAGAGGGCA TGACGAGCGC 
ATCTCCAGGT TTACCACGGT GGCGGATACC CCTGAGCTGC TACGGGCCCA GGCAGGGGGA 
CAGCTTCAAA GTGATGTGAG ATACACAGAA GACTATGAAC AACAGAGAGG AAAAGGCAGT 
TTCCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAGA GAGCCAATGA GCTGGCTAGC 
GATGTGAGGT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTAG TCCAGCTGCT 
GGAGCTGCAG GCACAAGGGA ATGCATGGAC CGATATGGCC AGCAGGGTTA CTTGGAGGAA 
TATCAGGAGC ACAGAGGAAA AGGCAGCTTC CCCGCCATGA TCACCCCAGC TTATCAGAAT 
GCCAAGAAAG CCAACGAACT CGCTAGTGAC ATAAAATACA GGCAGGACTT CAATAAGATG 
AAAGGCCCTG CACATTATCA CTCCCTCCCA GCCCAGGACA ACTTGGTTCT CAAGCGGGCC 
CAGAGCGTGA ACAAGCTTGT GAGCGAGGTG GAATATAAGA AGGACCTGGA AAGTAGTAAA 
GGTCACAGTA TCAACTACTG TGAGACGCCT CAATTCAGGA ACGTGAGCAA GATCTCAAAA 
TTCACCAGTG ATAATAAATA TAAAGAAAAT TACCAGAACC ACATGAGAGG CCACTATGAA 
GGAGTTGGTA TGGACAGACG GACTCTCCAT GCTATGAAGG TTGGAAGCTT GGCAAGCAAT 
ATTGCCTACA AGGCTGATTA TAAACACGAT GTTGTGGACT ACAACTACCC AGCCACTCTT 
ACTCCCTCTT ACCAGACAAC AATGAAACTT GTCCCCTTGA AAGATGTCAA CTACAGGCAA 
AGTATCGACA AGTTGAAGTA CAGCTCTGTG ACCAACACCC CTCAGATTAT CCAAGCCAAA 
ATCAATGCCC AACAACTGAG TCATGTGAAT TATCGTGCTG ACTATGAGAA AAATAAGTTG 
AACTACACCT TGCCACAGGA TGTACCTCAG CTGGTGAAGG CCAAAACCAA TGCTGAACTA 
TTTAGTGAGG TTAAGTATAA AGAAGGCTGG GAGAAGGCCA AGGGGAAAGG ATTTGAGATG 
AAGCTGGATG CTATGTCTTT GCTGGCCGCC AAAGCTTCTG GAGAGCTTGC TAGCAATATT 
AAATATAAAG AAGAGTATGA AAAAACGAAA GGCAAAGTCA TGGGCACTAC AGACTCGAGG 
CTTCTGCACT CTCTGCAGGT GGCCAAGATG AGCAGTGAGG TTGAATACAA GAAAGGCTTT 
GAAGAGAGTA AGACCCACTT TCACCTACCC ATGGATATGG TGCACATCAA GCATGCTAAG 
AAGGCCCAAG CTCTGGCCAG TGACCTGGAC TACAGGAAAA AGCTGCATGA ATACACTGTA 
CTGCCTGAAG ATATGAAGAC TCAGTGGGCC AAGAAAGCCT ATGGGCTCCA GAGTGAGCTG 
CAGTACAAGG CCGACCTAGC ATGGATGAAA GGAGTCGGGT GGCTGACGGA GGGGAGTCTC 
AATTTGGAAC AGGCCAAGAA GGCTGGCCAG CTGGCCAGCG AGAAAAACTA CAGGCAGAGG 
GTGGATGAGC TGAAGTTCAC CAGTGTGACC GACAGCTCCC AGATGGAGCA CGCCAAGAAG 
AGCCAGGAGC TGCAGAGCGG GGTGGCCTAC AAGGCAGGAA ATGAGCAGTC TGTCCATCAG 
TACAGCATCA GCAAAGATGA ACCTCTCTTC TTACAGGCCC GAGTCAATGC CGCCAATCTC 
AGTGAGAAAC TGTATAAGAG CAGCTGGGAA AACCAGAAGG CAAAAGGTTT TGAGCTGCGT 
CTGGACTCCT TGGGCTTCCT GGCAGCCAAA GCCAACAGGG ACCTGGCGAG CGAGGTGAAG 
TACAAGGAAG ATTACGAGAG GTCCCGAGGT AAGCTCATTG GAGCGAAGGA TGCCCAGGGG 
GATTCACAAA TGAGCCACTC GCTGCAAATG TCCAAGCTGC AGAGTGAACT CGAGTACAAG 
AAAGGGTTCG AGGACACCAG ATCCCAGTGC CACGTCTCCC TGGACATGAT CCACCTTGTG 
CATGCCCGCA AGGCTCAGCA TTTAGCCACA GACATAGGCT ACAAGACGGC ATCTCATCAG 
TTCACCGCTT TGCCCACCGA CATGAAGGTG GAATGGGCCA AGAAGGCATA CGGTTTACAG 
AGTGATAACC AATACAGGGC AGATGTGAAG TGGATGAAAG GCACGGGCTG GGTCGCCACC 
GGGTCATTAA ATGTGGAGCA GGCGAAGAAG GCAGGAGAAC TCATTAGCGA GAAGAAGTAC 
CGTCAGCATC CAGATGCTTT GAAGTTTACC AGTATTAAAG ACACTCCGGA GATGGTCCAG 
GCCAGAATTA GTTATACCCA AGCAGTGGAT AGGTTATACA GGGAACAAGG AGAAAATATA 
AAGCATCATT ACACTCAGAC AGCCGACCTC CCTGAAGTCC TGCTGGCCAA GCTGAATGCT 
ATGAATATCA GTGAGACGCG TTATAAGGAA TCCTGGAGCA AACTTCGGGA CGGTGGCTAT 
AAACTGAGGT TGGATGCCAT TCCGTTCCAG GCAGCAAAGG CTTCTGGCGA AATCATAAGT 
GATTACAAAT ACAAAGAGGC ATTTGAGAAA ATGAAAGGGC AGATGCTTGG CTCCCGGAGC 
TTGGAAGACG ATATCAGCCT TGCACATTCA GTGTATGCGA ACTCGCTGCA GAGCGAAGTG 
AATTACAAGA AAGGCTTTGA GCTCTCAAAG GCACACTTCC ATCTGCCGTT GGACATGGTA 
ACCCTGGTGC ATGCCAAGAA GGCTCAGAGC CTGGCCAGCG ACCAGGACTA CAGACACCCG 
CTCCCCCAGT ACACATCCTT GGCTGAAGAC CTGAGGCTCA GCTGCGCCAA GAAAGCTCAC 
AAGCTGCAGA GTGAGAATTT GTACCGCTCA GACCTGAACT TTATGCGAGG CGTTCCCTGT 
GTCATTCCGG GCACATTAGA GATTGAAGGG AGAAAGAAAG CATCTGAACT CATCAGTGAG 
AGTAAATACC GTCAGCATCC CCAGTCATTC AAGTACACAG CTGTGACAGA CACTCCCAGC 
CTCCTTCATG CAAAACTCAG CAACCAGATT ACCAATGAGC GCCTCTATAA AGCAGCCGGA 
GAGGATGAGA GGCACCGGTA CACAATGACC CTGGGTCTGC CCGAGTTTGT CCGAGCGAAA 
GCGAACGCAG CCAATCTGAG TGACGCAAAA TACAAGGAGT CTTGGCGTAA TCTCCGTGCT 
CAAGGCTACA AGCTGACAAT AGACGCTCTC CCCTTCCAGG CTGCTCGCGT CTCTGGAGAT 
ATAGCCAGTG ATTTTCTCTA CAGACATGAC TTTGTGAAGG AGCGAGGCAG GCTGATCGGG 
GCCCGGAGTG TGAGTGATGA CCCCCGACTC CAGCACTGCT GGCGGGTAGG CCAGTTGCAG 
AGCGAGCTTC AGTACAGGCG GGGAGCGGCG AGCAGCCAAG CCCACTACCA CCTGCCCATG 
GACATGGTGC ACCTGGTCCA TGCCAGGAAG GCCCAGGCCC TAGCCAGCGA CCACGACTAC 
AGGACACGGT GCCATGAGTT CACAGCTCTG CCTGAGGACC TGAAGATGTC CTGGGCCAAG 
AGAGCTCACG CCCTGCAGAG TGAGTTTCGC TACAAGTCAG ACCTGATGGG CATGAAGGGG 
ACAGGATGGC TGACGCTGAG TTCGCCGCAG CTAGAGAGTG CAAAGAAGGC CGGAGAACTC 
ATCAGCGAGA CCAAATACCG TAAGAAACCA GATAGTATCA AGTTCACCAC GGTGGTTGAC 
TCCCCAGACC TGGTTCACGC CAAGAACAGC TACATGCATT GCAATGAGCG CCTGTACAGA 
TTGGGGGATG CGGAATCCCT GCACAGATAT ACGCTGATCC CCGACCACCC CGATTTCACC 
AGAGCCCGCC TCAACGCGCT GCATCTGAGT GATAAAGTCT ACAGAAACTC TTGGGAACAG 
ACCCGGGCTG GTGGCTATGA CTTCCGGCTG GATGCCATCC CATTCCAGAC TGCCCGGGCG 
TCCCGGGAGA TCGCCAGTGA TTTCCGGTAC AAAGAGGCCT TCCTGCGGGA CCGGGGCCTA 
CAGATTGGTT ACCGCAGCAT CCACGACGAC CCCAGGATGA GGCATTTCCT CAGCGTCGGC 
AAACTCCAGA GTGACAACGA GTACAAGAAG GACTTTGCCA AGAGTCGGTC CCAGTTCCAT 
AGCCGCCCGG ACCAGCCCGG CTTCCTCCAG GCCAGGAGGA GCCAGCAGCT GGCCAGCGAC 
GTGTGCTACC GGCAGCCCCT GCCCCAGCCC ACATGCGACC CGGAGCAGCT AGGCCTGAAG 
CACGCACAGA AGGCCCACCA GCTGCAGAGT GACGTCAAGT ACAAATCAGA CTTGAACCTG 
ACCAGAGGCG TTGGCTGGAC ACCCCCCGGC TCCTACAAAG TGGAAATGGC TCGGCGGGCG 
GCAGAACTGG CCAAGGCGAG GGGCCTGGGT CTCCGGGGCG CTTGGGGGGG GCCAGAGGCT 
GTGGAGCCCG CAGAGAATCA GAGGGGGGAC GTGAACCCAG ACGCCACCGA GATTCTGCAC 
GTCAAAAGGA GGAAGGCTCT GCCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964422.1
CDS	224..5413
Translation	MNVQACSRCGYGVYPAEKISCLDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRKSPETIHGIDEQDEGERFKSVFHWDMKSRDGTAAPNRQPLMNERAYWNGYGEGNAWCPGAMPDPEIVRMVETRKSLGEEYTEDYEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKRGHDERISRFTTVADTPELLRAQAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMKGMASPAAGAAGTRECMDRYGQQGYLEEYQEHRGKGSFPAMITPAYQNAKKANELASDIKYRQDFNKMKGPAHYHSLPAQDNLVLKRAQSVNKLVSEVEYKKDLESSKGHSINYCETPQFRNVSKISKFTSDNKYKENYQNHMRGHYEGVGMDRRTLHAMKVGSLASNIAYKADYKHDVVDYNYPATLTPSYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIIQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAELFSEVKYKEGWEKAKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKVMGTTDSRLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVHIKHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLASEKNYRQRVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYSISKDEPLFLQARVNAANLSEKLYKSSWENQKAKGFELRLDSLGFLAAKANRDLASEVKYKEDYERSRGKLIGAKDAQGDSQMSHSLQMSKLQSELEYKKGFEDTRSQCHVSLDMIHLVHARKAQHLATDIGYKTASHQFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTADLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASGEIISDYKYKEAFEKMKGQMLGSRSLEDDISLAHSVYANSLQSEVNYKKGFELSKAHFHLPLDMVTLVHAKKAQSLASDQDYRHPLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVPCVIPGTLEIEGRKKASELISESKYRQHPQSFKYTAVTDTPSLLHAKLSNQITNERLYKAAGEDERHRYTMTLGLPEFVRAKANAANLSDAKYKESWRNLRAQGYKLTIDALPFQAARVSGDIASDFLYRHDFVKERGRLIGARSVSDDPRLQHCWRVGQLQSELQYRRGAASSQAHYHLPMDMVHLVHARKAQALASDHDYRTRCHEFTALPEDLKMSWAKRAHALQSEFRYKSDLMGMKGTGWLTLSSPQLESAKKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERLYRLGDAESLHRYTLIPDHPDFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGYRSIHDDPRMRHFLSVGKLQSDNEYKKDFAKSRSQFHSRPDQPGFLQARRSQQLASDVCYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELAKARGLGLRGAWGGPEAVEPAENQRGDVNPDATEILHVKRRKALPL

Target ORF information:

RefSeq Version	XM_014108947.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108947.2

ORF Insert Sequence:

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGGAGTA TACAGAAGAC 
TATGAACAAC AGAGGGGCAA AGGGAGCTTT CCAGCCATGA TCACACCTGC CTATCAAAGA 
GCCAAGAAAG CTAACCAGCT GGCCAGCCAA GTGGAATACA AGAGAGGGCA TGACGAGCGC 
ATCTCCAGGT TTACCACGGT GGCGGATACC CCTGAGCTGC TACGGGCCCA GGCAGGGGGA 
CAGCTTCAAA GTGATGTGAG ATACACAGAA GACTATGAAC AACAGAGAGG AAAAGGCAGT 
TTCCCTGCTA TGATCACGCC TGCTTATCAG ATAGCCAAGA GAGCCAATGA GCTGGCTAGC 
GATGTGAGGT ACCATCAACA ATATCAAAGA GAAATGAAGG GAATGGCTAG TCCAGCTGCT 
GGAGCTGCAG GCACAAGGGA ATGCATGGAC CGATATGGCC AGCAGGGTTA CTTGGAGGAA 
TATCAGGAGC ACAGAGGAAA AGGCAGCTTC CCCGCCATGA TCACCCCAGC TTATCAGAAT 
GCCAAGAAAG CCAACGAACT CGCTAGTGAC ATAAAATACA GGCAGGACTT CAATAAGATG 
AAAGGCCCTG CACATTATCA CTCCCTCCCA GCCCAGGACA ACTTGGTTCT CAAGCGGGCC 
CAGAGCGTGA ACAAGCTTGT GAGCGAGGTG GAATATAAGA AGGACCTGGA AAGTAGTAAA 
GGTCACAGTA TCAACTACTG TGAGACGCCT CAATTCAGGA ACGTGAGCAA GATCTCAAAA 
TTCACCAGTG ATAATAAATA TAAAGAAAAT TACCAGAACC ACATGAGAGG CCACTATGAA 
GGAGTTGGTA TGGACAGACG GACTCTCCAT GCTATGAAGG TTGGAAGCTT GGCAAGCAAT 
ATTGCCTACA AGGCTGATTA TAAACACGAT GTTGTGGACT ACAACTACCC AGCCACTCTT 
ACTCCCTCTT ACCAGACAAC AATGAAACTT GTCCCCTTGA AAGATGTCAA CTACAGGCAA 
AGTATCGACA AGTTGAAGTA CAGCTCTGTG ACCAACACCC CTCAGATTAT CCAAGCCAAA 
ATCAATGCCC AACAACTGAG TCATGTGAAT TATCGTGCTG ACTATGAGAA AAATAAGTTG 
AACTACACCT TGCCACAGGA TGTACCTCAG CTGGTGAAGG CCAAAACCAA TGCTGAACTA 
TTTAGTGAGG TTAAGTATAA AGAAGGCTGG GAGAAGGCCA AGGGGAAAGG ATTTGAGATG 
AAGCTGGATG CTATGTCTTT GCTGGCCGCC AAAGCTTCTG GAGAGCTTGC TAGCAATATT 
AAATATAAAG AAGAGTATGA AAAAACGAAA GGCAAAGTCA TGGGCACTAC AGACTCGAGG 
CTTCTGCACT CTCTGCAGGT GGCCAAGATG AGCAGTGAGG TTGAATACAA GAAAGGCTTT 
GAAGAGAGTA AGACCCACTT TCACCTACCC ATGGATATGG TGCACATCAA GCATGCTAAG 
AAGGCCCAAG CTCTGGCCAG TGACCTGGAC TACAGGAAAA AGCTGCATGA ATACACTGTA 
CTGCCTGAAG ATATGAAGAC TCAGTGGGCC AAGAAAGCCT ATGGGCTCCA GAGTGAGCTG 
CAGTACAAGG CCGACCTAGC ATGGATGAAA GGAGTCGGGT GGCTGACGGA GGGGAGTCTC 
AATTTGGAAC AGGCCAAGAA GGCTGGCCAG CTGGCCAGCG AGAAAAACTA CAGGCAGAGG 
GTGGATGAGC TGAAGTTCAC CAGTGTGACC GACAGCTCCC AGATGGAGCA CGCCAAGAAG 
AGCCAGGAGC TGCAGAGCGG GGTGGCCTAC AAGGCAGGAA ATGAGCAGTC TGTCCATCAG 
TACAGCATCA GCAAAGATGA ACCTCTCTTC TTACAGGCCC GAGTCAATGC CGCCAATCTC 
AGTGAGAAAC TGTATAAGAG CAGCTGGGAA AACCAGAAGG CAAAAGGTTT TGAGCTGCGT 
CTGGACTCCT TGGGCTTCCT GGCAGCCAAA GCCAACAGGG ACCTGGCGAG CGAGGTGAAG 
TACAAGGAAG ATTACGAGAG GTCCCGAGGT AAGCTCATTG GAGCGAAGGA TGCCCAGGGG 
GATTCACAAA TGAGCCACTC GCTGCAAATG TCCAAGCTGC AGAGTGAACT CGAGTACAAG 
AAAGGGTTCG AGGACACCAG ATCCCAGTGC CACGTCTCCC TGGACATGAT CCACCTTGTG 
CATGCCCGCA AGGCTCAGCA TTTAGCCACA GACATAGGCT ACAAGACGGC ATCTCATCAG 
TTCACCGCTT TGCCCACCGA CATGAAGGTG GAATGGGCCA AGAAGGCATA CGGTTTACAG 
AGTGATAACC AATACAGGGC AGATGTGAAG TGGATGAAAG GCACGGGCTG GGTCGCCACC 
GGGTCATTAA ATGTGGAGCA GGCGAAGAAG GCAGGAGAAC TCATTAGCGA GAAGAAGTAC 
CGTCAGCATC CAGATGCTTT GAAGTTTACC AGTATTAAAG ACACTCCGGA GATGGTCCAG 
GCCAGAATTA GTTATACCCA AGCAGTGGAT AGGTTATACA GGGAACAAGG AGAAAATATA 
AAGCATCATT ACACTCAGAC AGCCGACCTC CCTGAAGTCC TGCTGGCCAA GCTGAATGCT 
ATGAATATCA GTGAGACGCG TTATAAGGAA TCCTGGAGCA AACTTCGGGA CGGTGGCTAT 
AAACTGAGGT TGGATGCCAT TCCGTTCCAG GCAGCAAAGG CTTCTGGCGA AATCATAAGT 
GATTACAAAT ACAAAGAGGC ATTTGAGAAA ATGAAAGGGC AGATGCTTGG CTCCCGGAGC 
TTGGAAGACG ATATCAGCCT TGCACATTCA GTGTATGCGA ACTCGCTGCA GAGCGAAGTG 
AATTACAAGA AAGGCTTTGA GCTCTCAAAG GCACACTTCC ATCTGCCGTT GGACATGGTA 
ACCCTGGTGC ATGCCAAGAA GGCTCAGAGC CTGGCCAGCG ACCAGGACTA CAGACACCCG 
CTCCCCCAGT ACACATCCTT GGCTGAAGAC CTGAGGCTCA GCTGCGCCAA GAAAGCTCAC 
AAGCTGCAGA GTGAGAATTT GTACCGCTCA GACCTGAACT TTATGCGAGG CGTTCCCTGT 
GTCATTCCGG GCACATTAGA GATTGAAGGG AGAAAGAAAG CATCTGAACT CATCAGTGAG 
AGTAAATACC GTCAGCATCC CCAGTCATTC AAGTACACAG CTGTGACAGA CACTCCCAGC 
CTCCTTCATG CAAAACTCAG CAACCAGATT ACCAATGAGC GCCTCTATAA AGCAGCCGGA 
GAGGATGAGA GGCACCGGTA CACAATGACC CTGGGTCTGC CCGAGTTTGT CCGAGCGAAA 
GCGAACGCAG CCAATCTGAG TGACGCAAAA TACAAGGAGT CTTGGCGTAA TCTCCGTGCT 
CAAGGCTACA AGCTGACAAT AGACGCTCTC CCCTTCCAGG CTGCTCGCGT CTCTGGAGAT 
ATAGCCAGTG ATTTTCTCTA CAGACATGAC TTTGTGAAGG AGCGAGGCAG GCTGATCGGG 
GCCCGGAGTG TGAGTGATGA CCCCCGACTC CAGCACTGCT GGCGGGTAGG CCAGTTGCAG 
AGCGAGCTTC AGTACAGGCG GGGAGCGGCG AGCAGCCAAG CCCACTACCA CCTGCCCATG 
GACATGGTGC ACCTGGTCCA TGCCAGGAAG GCCCAGGCCC TAGCCAGCGA CCACGACTAC 
AGGACACGGT GCCATGAGTT CACAGCTCTG CCTGAGGACC TGAAGATGTC CTGGGCCAAG 
AGAGCTCACG CCCTGCAGAG TGAGTTTCGC TACAAGTCAG ACCTGATGGG CATGAAGGGG 
ACAGGATGGC TGACGCTGAG TTCGCCGCAG CTAGAGAGTG CAAAGAAGGC CGGAGAACTC 
ATCAGCGAGA CCAAATACCG TAAGAAACCA GATAGTATCA AGTTCACCAC GGTGGTTGAC 
TCCCCAGACC TGGTTCACGC CAAGAACAGC TACATGCATT GCAATGAGCG CCTGTACAGA 
TTGGGGGATG CGGAATCCCT GCACAGATAT ACGCTGATCC CCGACCACCC CGATTTCACC 
AGAGCCCGCC TCAACGCGCT GCATCTGAGT GATAAAGTCT ACAGAAACTC TTGGGAACAG 
ACCCGGGCTG GTGGCTATGA CTTCCGGCTG GATGCCATCC CATTCCAGAC TGCCCGGGCG 
TCCCGGGAGA TCGCCAGTGA TTTCCGGTAC AAAGAGGCCT TCCTGCGGGA CCGGGGCCTA 
CAGATTGGTT ACCGCAGCAT CCACGACGAC CCCAGGATGA GGCATTTCCT CAGCGTCGGC 
AAACTCCAGA GTGACAACGA GTACAAGAAG GACTTTGCCA AGAGTCGGTC CCAGTTCCAT 
AGCCGCCCGG ACCAGCCCGG CTTCCTCCAG GCCAGGAGGA GCCAGCAGCT GGCCAGCGAC 
GTGTGCTACC GGCAGCCCCT GCCCCAGCCC ACATGCGACC CGGAGCAGCT AGGCCTGAAG 
CACGCACAGA AGGCCCACCA GCTGCAGAGT GACGTCAAGT ACAAATCAGA CTTGAACCTG 
ACCAGAGGCG TTGGCTGGAC ACCCCCCGGC TCCTACAAAG TGGAAATGGC TCGGCGGGCG 
GCAGAACTGG CCAAGGCGAG GGGCCTGGGT CTCCGGGGCG CTTGGGGGGG GCCAGAGGCT 
GTGGAGCCCG CAGAGAATCA GAGGGGGGAC GTGAACCCAG ACGCCACCGA GATTCTGCAC 
GTCAAAAGGA GGAAGGCTCT GCCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_014108949.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964424.1 Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X5, mRNA.

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CloneID	OCe43947
Clone ID Related Accession (Same CDS sequence)	XM_014108949.2 , XM_014108949.1
Accession Version	XM_014108949.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	nebulin-related-anchoring protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006610.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108949.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGCAGGA GTATACAGAA 
GACTATGAAC AACAGAGGGG CAAAGGGAGC TTTCCAGCCA TGATCACACC TGCCTATCAA 
AGAGCCAAGA AAGCTAACCA GCTGGCCAGC CAAGTGGAAT ACAAGAGAGG GCATGACGAG 
CGCATCTCCA GGTTTACCAC GGTGGCGGAT ACCCCTGAGC TGCTACGGGC CCAGGCAGGG 
GGACAGCTTC AAAGTGATGT GAGATACACA GAAGACTATG AACAACAGAG AGGAAAAGGC 
AGTTTCCCTG CTATGATCAC GCCTGCTTAT CAGATAGCCA AGAGAGCCAA TGAGCTGGCT 
AGCGATGTGA GGTACCATCA ACAATATCAA AGAGAAATGA AGGGAATGGC TAGTCCAGCT 
GCTGGAGCTG CAGGCACAAG GGAATGCATG GACCGATATG GCCAGCAGGG TTACTTGGAG 
GAATATCAGG AGCACAGAGG AAAAGGCAGC TTCCCCGCCA TGATCACCCC AGCTTATCAG 
AATGCCAAGA AAGCCAACGA ACTCGCTAGT GACATAAAAT ACAGGCAGGA CTTCAATAAG 
ATGAAAGGCC CTGCACATTA TCACTCCCTC CCAGCCCAGG ACAACTTGGT TCTCAAGCGG 
GCCCAGAGCG TGAACAAGCT TGTGAGCGAG AATAAATATA AAGAAAATTA CCAGAACCAC 
ATGAGAGGCC ACTATGAAGG AGTTGGTATG GACAGACGGA CTCTCCATGC TATGAAGGTT 
GGAAGCTTGG CAAGCAATAT TGCCTACAAG GCTGATTATA AACACGATGT TGTGGACTAC 
AACTACCCAG CCACTCTTAC TCCCTCTTAC CAGACAACAA TGAAACTTGT CCCCTTGAAA 
GATGTCAACT ACAGGCAAAG TATCGACAAG TTGAAGTACA GCTCTGTGAC CAACACCCCT 
CAGATTATCC AAGCCAAAAT CAATGCCCAA CAACTGAGTC ATGTGAATTA TCGTGCTGAC 
TATGAGAAAA ATAAGTTGAA CTACACCTTG CCACAGGATG TACCTCAGCT GGTGAAGGCC 
AAAACCAATG CTGAACTATT TAGTGAGGTT AAGTATAAAG AAGGCTGGGA GAAGGCCAAG 
GGGAAAGGAT TTGAGATGAA GCTGGATGCT ATGTCTTTGC TGGCCGCCAA AGCTTCTGGA 
GAGCTTGCTA GCAATATTAA ATATAAAGAA GAGTATGAAA AAACGAAAGG CAAAGTCATG 
GGCACTACAG ACTCGAGGCT TCTGCACTCT CTGCAGGTGG CCAAGATGAG CAGTGAGGTT 
GAATACAAGA AAGGCTTTGA AGAGAGTAAG ACCCACTTTC ACCTACCCAT GGATATGGTG 
CACATCAAGC ATGCTAAGAA GGCCCAAGCT CTGGCCAGTG ACCTGGACTA CAGGAAAAAG 
CTGCATGAAT ACACTGTACT GCCTGAAGAT ATGAAGACTC AGTGGGCCAA GAAAGCCTAT 
GGGCTCCAGA GTGAGCTGCA GTACAAGGCC GACCTAGCAT GGATGAAAGG AGTCGGGTGG 
CTGACGGAGG GGAGTCTCAA TTTGGAACAG GCCAAGAAGG CTGGCCAGCT GGCCAGCGAG 
AAAAACTACA GGCAGAGGGT GGATGAGCTG AAGTTCACCA GTGTGACCGA CAGCTCCCAG 
ATGGAGCACG CCAAGAAGAG CCAGGAGCTG CAGAGCGGGG TGGCCTACAA GGCAGGAAAT 
GAGCAGTCTG TCCATCAGTA CAGCATCAGC AAAGATGAAC CTCTCTTCTT ACAGGCCCGA 
GTCAATGCCG CCAATCTCAG TGAGAAACTG TATAAGAGCA GCTGGGAAAA CCAGAAGGCA 
AAAGGTTTTG AGCTGCGTCT GGACTCCTTG GGCTTCCTGG CAGCCAAAGC CAACAGGGAC 
CTGGCGAGCG AGGTGAAGTA CAAGGAAGAT TACGAGAGGT CCCGAGGTAA GCTCATTGGA 
GCGAAGGATG CCCAGGGGGA TTCACAAATG AGCCACTCGC TGCAAATGTC CAAGCTGCAG 
AGTGAACTCG AGTACAAGAA AGGGTTCGAG GACACCAGAT CCCAGTGCCA CGTCTCCCTG 
GACATGATCC ACCTTGTGCA TGCCCGCAAG GCTCAGCATT TAGCCACAGA CATAGGCTAC 
AAGACGGCAT CTCATCAGTT CACCGCTTTG CCCACCGACA TGAAGGTGGA ATGGGCCAAG 
AAGGCATACG GTTTACAGAG TGATAACCAA TACAGGGCAG ATGTGAAGTG GATGAAAGGC 
ACGGGCTGGG TCGCCACCGG GTCATTAAAT GTGGAGCAGG CGAAGAAGGC AGGAGAACTC 
ATTAGCGAGA AGAAGTACCG TCAGCATCCA GATGCTTTGA AGTTTACCAG TATTAAAGAC 
ACTCCGGAGA TGGTCCAGGC CAGAATTAGT TATACCCAAG CAGTGGATAG GTTATACAGG 
GAACAAGGAG AAAATATAAA GCATCATTAC ACTCAGACAG CCGACCTCCC TGAAGTCCTG 
CTGGCCAAGC TGAATGCTAT GAATATCAGT GAGACGCGTT ATAAGGAATC CTGGAGCAAA 
CTTCGGGACG GTGGCTATAA ACTGAGGTTG GATGCCATTC CGTTCCAGGC AGCAAAGGCT 
TCTGGCGAAA TCATAAGTGA TTACAAATAC AAAGAGGCAT TTGAGAAAAT GAAAGGGCAG 
ATGCTTGGCT CCCGGAGCTT GGAAGACGAT ATCAGCCTTG CACATTCAGT GTATGCGAAC 
TCGCTGCAGA GCGAAGTGAA TTACAAGAAA GGCTTTGAGC TCTCAAAGGC ACACTTCCAT 
CTGCCGTTGG ACATGGTAAC CCTGGTGCAT GCCAAGAAGG CTCAGAGCCT GGCCAGCGAC 
CAGGACTACA GACACCCGCT CCCCCAGTAC ACATCCTTGG CTGAAGACCT GAGGCTCAGC 
TGCGCCAAGA AAGCTCACAA GCTGCAGAGT GAGAATTTGT ACCGCTCAGA CCTGAACTTT 
ATGCGAGGCG TTCCCTGTGT CATTCCGGGC ACATTAGAGA TTGAAGGGAG AAAGAAAGCA 
TCTGAACTCA TCAGTGAGAG TAAATACCGT CAGCATCCCC AGTCATTCAA GTACACAGCT 
GTGACAGACA CTCCCAGCCT CCTTCATGCA AAACTCAGCA ACCAGATTAC CAATGAGCGC 
CTCTATAAAG CAGCCGGAGA GGATGAGAGG CACCGGTACA CAATGACCCT GGGTCTGCCC 
GAGTTTGTCC GAGCGAAAGC GAACGCAGCC AATCTGAGTG ACGCAAAATA CAAGGAGTCT 
TGGCGTAATC TCCGTGCTCA AGGCTACAAG CTGACAATAG ACGCTCTCCC CTTCCAGGCT 
GCTCGCGTCT CTGGAGATAT AGCCAGTGAT TTTCTCTACA GACATGACTT TGTGAAGGAG 
CGAGGCAGGC TGATCGGGGC CCGGAGTGTG AGTGATGACC CCCGACTCCA GCACTGCTGG 
CGGGTAGGCC AGTTGCAGAG CGAGCTTCAG TACAGGCGGG GAGCGGCGAG CAGCCAAGCC 
CACTACCACC TGCCCATGGA CATGGTGCAC CTGGTCCATG CCAGGAAGGC CCAGGCCCTA 
GCCAGCGACC ACGACTACAG GACACGGTGC CATGAGTTCA CAGCTCTGCC TGAGGACCTG 
AAGATGTCCT GGGCCAAGAG AGCTCACGCC CTGCAGAGTG AGTTTCGCTA CAAGTCAGAC 
CTGATGGGCA TGAAGGGGAC AGGATGGCTG ACGCTGAGTT CGCCGCAGCT AGAGAGTGCA 
AAGAAGGCCG GAGAACTCAT CAGCGAGACC AAATACCGTA AGAAACCAGA TAGTATCAAG 
TTCACCACGG TGGTTGACTC CCCAGACCTG GTTCACGCCA AGAACAGCTA CATGCATTGC 
AATGAGCGCC TGTACAGATT GGGGGATGCG GAATCCCTGC ACAGATATAC GCTGATCCCC 
GACCACCCCG ATTTCACCAG AGCCCGCCTC AACGCGCTGC ATCTGAGTGA TAAAGTCTAC 
AGAAACTCTT GGGAACAGAC CCGGGCTGGT GGCTATGACT TCCGGCTGGA TGCCATCCCA 
TTCCAGACTG CCCGGGCGTC CCGGGAGATC GCCAGTGATT TCCGGTACAA AGAGGCCTTC 
CTGCGGGACC GGGGCCTACA GATTGGTTAC CGCAGCATCC ACGACGACCC CAGGATGAGG 
CATTTCCTCA GCGTCGGCAA ACTCCAGAGT GACAACGAGT ACAAGAAGGA CTTTGCCAAG 
AGTCGGTCCC AGTTCCATAG CCGCCCGGAC CAGCCCGGCT TCCTCCAGGC CAGGAGGAGC 
CAGCAGCTGG CCAGCGACGT GTGCTACCGG CAGCCCCTGC CCCAGCCCAC ATGCGACCCG 
GAGCAGCTAG GCCTGAAGCA CGCACAGAAG GCCCACCAGC TGCAGAGTGA CGTCAAGTAC 
AAATCAGACT TGAACCTGAC CAGAGGCGTT GGCTGGACAC CCCCCGGCTC CTACAAAGTG 
GAAATGGCTC GGCGGGCGGC AGAACTGGCC AAGGCGAGGG GCCTGGGTCT CCGGGGCGCT 
TGGGGGGGGC CAGAGGCTGT GGAGCCCGCA GAGAATCAGA GGGGGGACGT GAACCCAGAC 
GCCACCGAGA TTCTGCACGT CAAAAGGAGG AAGGCTCTGC CGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964424.1
CDS	224..5311
Translation	MNVQACSRCGYGVYPAEKISCLDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRKSPETIHGIDEQDEGERFKSVFHWDMKSRDGTAAPNRQPLMNERAYWNGYGEGNAWCPGAMPDPEIVRMVETRKSLGEQEYTEDYEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKRGHDERISRFTTVADTPELLRAQAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMKGMASPAAGAAGTRECMDRYGQQGYLEEYQEHRGKGSFPAMITPAYQNAKKANELASDIKYRQDFNKMKGPAHYHSLPAQDNLVLKRAQSVNKLVSENKYKENYQNHMRGHYEGVGMDRRTLHAMKVGSLASNIAYKADYKHDVVDYNYPATLTPSYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIIQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAELFSEVKYKEGWEKAKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKVMGTTDSRLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVHIKHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLASEKNYRQRVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYSISKDEPLFLQARVNAANLSEKLYKSSWENQKAKGFELRLDSLGFLAAKANRDLASEVKYKEDYERSRGKLIGAKDAQGDSQMSHSLQMSKLQSELEYKKGFEDTRSQCHVSLDMIHLVHARKAQHLATDIGYKTASHQFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTADLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASGEIISDYKYKEAFEKMKGQMLGSRSLEDDISLAHSVYANSLQSEVNYKKGFELSKAHFHLPLDMVTLVHAKKAQSLASDQDYRHPLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVPCVIPGTLEIEGRKKASELISESKYRQHPQSFKYTAVTDTPSLLHAKLSNQITNERLYKAAGEDERHRYTMTLGLPEFVRAKANAANLSDAKYKESWRNLRAQGYKLTIDALPFQAARVSGDIASDFLYRHDFVKERGRLIGARSVSDDPRLQHCWRVGQLQSELQYRRGAASSQAHYHLPMDMVHLVHARKAQALASDHDYRTRCHEFTALPEDLKMSWAKRAHALQSEFRYKSDLMGMKGTGWLTLSSPQLESAKKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERLYRLGDAESLHRYTLIPDHPDFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGYRSIHDDPRMRHFLSVGKLQSDNEYKKDFAKSRSQFHSRPDQPGFLQARRSQQLASDVCYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELAKARGLGLRGAWGGPEAVEPAENQRGDVNPDATEILHVKRRKALPL

Target ORF information:

RefSeq Version	XM_014108949.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nebulin related anchoring protein (NRAP), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108949.2

ORF Insert Sequence:

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGCAGGA GTATACAGAA 
GACTATGAAC AACAGAGGGG CAAAGGGAGC TTTCCAGCCA TGATCACACC TGCCTATCAA 
AGAGCCAAGA AAGCTAACCA GCTGGCCAGC CAAGTGGAAT ACAAGAGAGG GCATGACGAG 
CGCATCTCCA GGTTTACCAC GGTGGCGGAT ACCCCTGAGC TGCTACGGGC CCAGGCAGGG 
GGACAGCTTC AAAGTGATGT GAGATACACA GAAGACTATG AACAACAGAG AGGAAAAGGC 
AGTTTCCCTG CTATGATCAC GCCTGCTTAT CAGATAGCCA AGAGAGCCAA TGAGCTGGCT 
AGCGATGTGA GGTACCATCA ACAATATCAA AGAGAAATGA AGGGAATGGC TAGTCCAGCT 
GCTGGAGCTG CAGGCACAAG GGAATGCATG GACCGATATG GCCAGCAGGG TTACTTGGAG 
GAATATCAGG AGCACAGAGG AAAAGGCAGC TTCCCCGCCA TGATCACCCC AGCTTATCAG 
AATGCCAAGA AAGCCAACGA ACTCGCTAGT GACATAAAAT ACAGGCAGGA CTTCAATAAG 
ATGAAAGGCC CTGCACATTA TCACTCCCTC CCAGCCCAGG ACAACTTGGT TCTCAAGCGG 
GCCCAGAGCG TGAACAAGCT TGTGAGCGAG AATAAATATA AAGAAAATTA CCAGAACCAC 
ATGAGAGGCC ACTATGAAGG AGTTGGTATG GACAGACGGA CTCTCCATGC TATGAAGGTT 
GGAAGCTTGG CAAGCAATAT TGCCTACAAG GCTGATTATA AACACGATGT TGTGGACTAC 
AACTACCCAG CCACTCTTAC TCCCTCTTAC CAGACAACAA TGAAACTTGT CCCCTTGAAA 
GATGTCAACT ACAGGCAAAG TATCGACAAG TTGAAGTACA GCTCTGTGAC CAACACCCCT 
CAGATTATCC AAGCCAAAAT CAATGCCCAA CAACTGAGTC ATGTGAATTA TCGTGCTGAC 
TATGAGAAAA ATAAGTTGAA CTACACCTTG CCACAGGATG TACCTCAGCT GGTGAAGGCC 
AAAACCAATG CTGAACTATT TAGTGAGGTT AAGTATAAAG AAGGCTGGGA GAAGGCCAAG 
GGGAAAGGAT TTGAGATGAA GCTGGATGCT ATGTCTTTGC TGGCCGCCAA AGCTTCTGGA 
GAGCTTGCTA GCAATATTAA ATATAAAGAA GAGTATGAAA AAACGAAAGG CAAAGTCATG 
GGCACTACAG ACTCGAGGCT TCTGCACTCT CTGCAGGTGG CCAAGATGAG CAGTGAGGTT 
GAATACAAGA AAGGCTTTGA AGAGAGTAAG ACCCACTTTC ACCTACCCAT GGATATGGTG 
CACATCAAGC ATGCTAAGAA GGCCCAAGCT CTGGCCAGTG ACCTGGACTA CAGGAAAAAG 
CTGCATGAAT ACACTGTACT GCCTGAAGAT ATGAAGACTC AGTGGGCCAA GAAAGCCTAT 
GGGCTCCAGA GTGAGCTGCA GTACAAGGCC GACCTAGCAT GGATGAAAGG AGTCGGGTGG 
CTGACGGAGG GGAGTCTCAA TTTGGAACAG GCCAAGAAGG CTGGCCAGCT GGCCAGCGAG 
AAAAACTACA GGCAGAGGGT GGATGAGCTG AAGTTCACCA GTGTGACCGA CAGCTCCCAG 
ATGGAGCACG CCAAGAAGAG CCAGGAGCTG CAGAGCGGGG TGGCCTACAA GGCAGGAAAT 
GAGCAGTCTG TCCATCAGTA CAGCATCAGC AAAGATGAAC CTCTCTTCTT ACAGGCCCGA 
GTCAATGCCG CCAATCTCAG TGAGAAACTG TATAAGAGCA GCTGGGAAAA CCAGAAGGCA 
AAAGGTTTTG AGCTGCGTCT GGACTCCTTG GGCTTCCTGG CAGCCAAAGC CAACAGGGAC 
CTGGCGAGCG AGGTGAAGTA CAAGGAAGAT TACGAGAGGT CCCGAGGTAA GCTCATTGGA 
GCGAAGGATG CCCAGGGGGA TTCACAAATG AGCCACTCGC TGCAAATGTC CAAGCTGCAG 
AGTGAACTCG AGTACAAGAA AGGGTTCGAG GACACCAGAT CCCAGTGCCA CGTCTCCCTG 
GACATGATCC ACCTTGTGCA TGCCCGCAAG GCTCAGCATT TAGCCACAGA CATAGGCTAC 
AAGACGGCAT CTCATCAGTT CACCGCTTTG CCCACCGACA TGAAGGTGGA ATGGGCCAAG 
AAGGCATACG GTTTACAGAG TGATAACCAA TACAGGGCAG ATGTGAAGTG GATGAAAGGC 
ACGGGCTGGG TCGCCACCGG GTCATTAAAT GTGGAGCAGG CGAAGAAGGC AGGAGAACTC 
ATTAGCGAGA AGAAGTACCG TCAGCATCCA GATGCTTTGA AGTTTACCAG TATTAAAGAC 
ACTCCGGAGA TGGTCCAGGC CAGAATTAGT TATACCCAAG CAGTGGATAG GTTATACAGG 
GAACAAGGAG AAAATATAAA GCATCATTAC ACTCAGACAG CCGACCTCCC TGAAGTCCTG 
CTGGCCAAGC TGAATGCTAT GAATATCAGT GAGACGCGTT ATAAGGAATC CTGGAGCAAA 
CTTCGGGACG GTGGCTATAA ACTGAGGTTG GATGCCATTC CGTTCCAGGC AGCAAAGGCT 
TCTGGCGAAA TCATAAGTGA TTACAAATAC AAAGAGGCAT TTGAGAAAAT GAAAGGGCAG 
ATGCTTGGCT CCCGGAGCTT GGAAGACGAT ATCAGCCTTG CACATTCAGT GTATGCGAAC 
TCGCTGCAGA GCGAAGTGAA TTACAAGAAA GGCTTTGAGC TCTCAAAGGC ACACTTCCAT 
CTGCCGTTGG ACATGGTAAC CCTGGTGCAT GCCAAGAAGG CTCAGAGCCT GGCCAGCGAC 
CAGGACTACA GACACCCGCT CCCCCAGTAC ACATCCTTGG CTGAAGACCT GAGGCTCAGC 
TGCGCCAAGA AAGCTCACAA GCTGCAGAGT GAGAATTTGT ACCGCTCAGA CCTGAACTTT 
ATGCGAGGCG TTCCCTGTGT CATTCCGGGC ACATTAGAGA TTGAAGGGAG AAAGAAAGCA 
TCTGAACTCA TCAGTGAGAG TAAATACCGT CAGCATCCCC AGTCATTCAA GTACACAGCT 
GTGACAGACA CTCCCAGCCT CCTTCATGCA AAACTCAGCA ACCAGATTAC CAATGAGCGC 
CTCTATAAAG CAGCCGGAGA GGATGAGAGG CACCGGTACA CAATGACCCT GGGTCTGCCC 
GAGTTTGTCC GAGCGAAAGC GAACGCAGCC AATCTGAGTG ACGCAAAATA CAAGGAGTCT 
TGGCGTAATC TCCGTGCTCA AGGCTACAAG CTGACAATAG ACGCTCTCCC CTTCCAGGCT 
GCTCGCGTCT CTGGAGATAT AGCCAGTGAT TTTCTCTACA GACATGACTT TGTGAAGGAG 
CGAGGCAGGC TGATCGGGGC CCGGAGTGTG AGTGATGACC CCCGACTCCA GCACTGCTGG 
CGGGTAGGCC AGTTGCAGAG CGAGCTTCAG TACAGGCGGG GAGCGGCGAG CAGCCAAGCC 
CACTACCACC TGCCCATGGA CATGGTGCAC CTGGTCCATG CCAGGAAGGC CCAGGCCCTA 
GCCAGCGACC ACGACTACAG GACACGGTGC CATGAGTTCA CAGCTCTGCC TGAGGACCTG 
AAGATGTCCT GGGCCAAGAG AGCTCACGCC CTGCAGAGTG AGTTTCGCTA CAAGTCAGAC 
CTGATGGGCA TGAAGGGGAC AGGATGGCTG ACGCTGAGTT CGCCGCAGCT AGAGAGTGCA 
AAGAAGGCCG GAGAACTCAT CAGCGAGACC AAATACCGTA AGAAACCAGA TAGTATCAAG 
TTCACCACGG TGGTTGACTC CCCAGACCTG GTTCACGCCA AGAACAGCTA CATGCATTGC 
AATGAGCGCC TGTACAGATT GGGGGATGCG GAATCCCTGC ACAGATATAC GCTGATCCCC 
GACCACCCCG ATTTCACCAG AGCCCGCCTC AACGCGCTGC ATCTGAGTGA TAAAGTCTAC 
AGAAACTCTT GGGAACAGAC CCGGGCTGGT GGCTATGACT TCCGGCTGGA TGCCATCCCA 
TTCCAGACTG CCCGGGCGTC CCGGGAGATC GCCAGTGATT TCCGGTACAA AGAGGCCTTC 
CTGCGGGACC GGGGCCTACA GATTGGTTAC CGCAGCATCC ACGACGACCC CAGGATGAGG 
CATTTCCTCA GCGTCGGCAA ACTCCAGAGT GACAACGAGT ACAAGAAGGA CTTTGCCAAG 
AGTCGGTCCC AGTTCCATAG CCGCCCGGAC CAGCCCGGCT TCCTCCAGGC CAGGAGGAGC 
CAGCAGCTGG CCAGCGACGT GTGCTACCGG CAGCCCCTGC CCCAGCCCAC ATGCGACCCG 
GAGCAGCTAG GCCTGAAGCA CGCACAGAAG GCCCACCAGC TGCAGAGTGA CGTCAAGTAC 
AAATCAGACT TGAACCTGAC CAGAGGCGTT GGCTGGACAC CCCCCGGCTC CTACAAAGTG 
GAAATGGCTC GGCGGGCGGC AGAACTGGCC AAGGCGAGGG GCCTGGGTCT CCGGGGCGCT 
TGGGGGGGGC CAGAGGCTGT GGAGCCCGCA GAGAATCAGA GGGGGGACGT GAACCCAGAC 
GCCACCGAGA TTCTGCACGT CAAAAGGAGG AAGGCTCTGC CGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAP ( XM_014108949.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964424.1 Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X5, mRNA.

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CloneID	OCe43947
Clone ID Related Accession (Same CDS sequence)	XM_014108949.2 , XM_014108949.1
Accession Version	XM_014108949.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	nebulin-related-anchoring protein isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726109.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGCAGGA GTATACAGAA 
GACTATGAAC AACAGAGGGG CAAAGGGAGC TTTCCAGCCA TGATCACACC TGCCTATCAA 
AGAGCCAAGA AAGCTAACCA GCTGGCCAGC CAAGTGGAAT ACAAGAGAGG GCATGACGAG 
CGCATCTCCA GGTTTACCAC GGTGGCGGAT ACCCCTGAGC TGCTACGGGC CCAGGCAGGG 
GGACAGCTTC AAAGTGATGT GAGATACACA GAAGACTATG AACAACAGAG AGGAAAAGGC 
AGTTTCCCTG CTATGATCAC GCCTGCTTAT CAGATAGCCA AGAGAGCCAA TGAGCTGGCT 
AGCGATGTGA GGTACCATCA ACAATATCAA AGAGAAATGA AGGGAATGGC TAGTCCAGCT 
GCTGGAGCTG CAGGCACAAG GGAATGCATG GACCGATATG GCCAGCAGGG TTACTTGGAG 
GAATATCAGG AGCACAGAGG AAAAGGCAGC TTCCCCGCCA TGATCACCCC AGCTTATCAG 
AATGCCAAGA AAGCCAACGA ACTCGCTAGT GACATAAAAT ACAGGCAGGA CTTCAATAAG 
ATGAAAGGCC CTGCACATTA TCACTCCCTC CCAGCCCAGG ACAACTTGGT TCTCAAGCGG 
GCCCAGAGCG TGAACAAGCT TGTGAGCGAG AATAAATATA AAGAAAATTA CCAGAACCAC 
ATGAGAGGCC ACTATGAAGG AGTTGGTATG GACAGACGGA CTCTCCATGC TATGAAGGTT 
GGAAGCTTGG CAAGCAATAT TGCCTACAAG GCTGATTATA AACACGATGT TGTGGACTAC 
AACTACCCAG CCACTCTTAC TCCCTCTTAC CAGACAACAA TGAAACTTGT CCCCTTGAAA 
GATGTCAACT ACAGGCAAAG TATCGACAAG TTGAAGTACA GCTCTGTGAC CAACACCCCT 
CAGATTATCC AAGCCAAAAT CAATGCCCAA CAACTGAGTC ATGTGAATTA TCGTGCTGAC 
TATGAGAAAA ATAAGTTGAA CTACACCTTG CCACAGGATG TACCTCAGCT GGTGAAGGCC 
AAAACCAATG CTGAACTATT TAGTGAGGTT AAGTATAAAG AAGGCTGGGA GAAGGCCAAG 
GGGAAAGGAT TTGAGATGAA GCTGGATGCT ATGTCTTTGC TGGCCGCCAA AGCTTCTGGA 
GAGCTTGCTA GCAATATTAA ATATAAAGAA GAGTATGAAA AAACGAAAGG CAAAGTCATG 
GGCACTACAG ACTCGAGGCT TCTGCACTCT CTGCAGGTGG CCAAGATGAG CAGTGAGGTT 
GAATACAAGA AAGGCTTTGA AGAGAGTAAG ACCCACTTTC ACCTACCCAT GGATATGGTG 
CACATCAAGC ATGCTAAGAA GGCCCAAGCT CTGGCCAGTG ACCTGGACTA CAGGAAAAAG 
CTGCATGAAT ACACTGTACT GCCTGAAGAT ATGAAGACTC AGTGGGCCAA GAAAGCCTAT 
GGGCTCCAGA GTGAGCTGCA GTACAAGGCC GACCTAGCAT GGATGAAAGG AGTCGGGTGG 
CTGACGGAGG GGAGTCTCAA TTTGGAACAG GCCAAGAAGG CTGGCCAGCT GGCCAGCGAG 
AAAAACTACA GGCAGAGGGT GGATGAGCTG AAGTTCACCA GTGTGACCGA CAGCTCCCAG 
ATGGAGCACG CCAAGAAGAG CCAGGAGCTG CAGAGCGGGG TGGCCTACAA GGCAGGAAAT 
GAGCAGTCTG TCCATCAGTA CAGCATCAGC AAAGATGAAC CTCTCTTCTT ACAGGCCCGA 
GTCAATGCCG CCAATCTCAG TGAGAAACTG TATAAGAGCA GCTGGGAAAA CCAGAAGGCA 
AAAGGTTTTG AGCTGCGTCT GGACTCCTTG GGCTTCCTGG CAGCCAAAGC CAACAGGGAC 
CTGGCGAGCG AGGTGAAGTA CAAGGAAGAT TACGAGAGGT CCCGAGGTAA GCTCATTGGA 
GCGAAGGATG CCCAGGGGGA TTCACAAATG AGCCACTCGC TGCAAATGTC CAAGCTGCAG 
AGTGAACTCG AGTACAAGAA AGGGTTCGAG GACACCAGAT CCCAGTGCCA CGTCTCCCTG 
GACATGATCC ACCTTGTGCA TGCCCGCAAG GCTCAGCATT TAGCCACAGA CATAGGCTAC 
AAGACGGCAT CTCATCAGTT CACCGCTTTG CCCACCGACA TGAAGGTGGA ATGGGCCAAG 
AAGGCATACG GTTTACAGAG TGATAACCAA TACAGGGCAG ATGTGAAGTG GATGAAAGGC 
ACGGGCTGGG TCGCCACCGG GTCATTAAAT GTGGAGCAGG CGAAGAAGGC AGGAGAACTC 
ATTAGCGAGA AGAAGTACCG TCAGCATCCA GATGCTTTGA AGTTTACCAG TATTAAAGAC 
ACTCCGGAGA TGGTCCAGGC CAGAATTAGT TATACCCAAG CAGTGGATAG GTTATACAGG 
GAACAAGGAG AAAATATAAA GCATCATTAC ACTCAGACAG CCGACCTCCC TGAAGTCCTG 
CTGGCCAAGC TGAATGCTAT GAATATCAGT GAGACGCGTT ATAAGGAATC CTGGAGCAAA 
CTTCGGGACG GTGGCTATAA ACTGAGGTTG GATGCCATTC CGTTCCAGGC AGCAAAGGCT 
TCTGGCGAAA TCATAAGTGA TTACAAATAC AAAGAGGCAT TTGAGAAAAT GAAAGGGCAG 
ATGCTTGGCT CCCGGAGCTT GGAAGACGAT ATCAGCCTTG CACATTCAGT GTATGCGAAC 
TCGCTGCAGA GCGAAGTGAA TTACAAGAAA GGCTTTGAGC TCTCAAAGGC ACACTTCCAT 
CTGCCGTTGG ACATGGTAAC CCTGGTGCAT GCCAAGAAGG CTCAGAGCCT GGCCAGCGAC 
CAGGACTACA GACACCCGCT CCCCCAGTAC ACATCCTTGG CTGAAGACCT GAGGCTCAGC 
TGCGCCAAGA AAGCTCACAA GCTGCAGAGT GAGAATTTGT ACCGCTCAGA CCTGAACTTT 
ATGCGAGGCG TTCCCTGTGT CATTCCGGGC ACATTAGAGA TTGAAGGGAG AAAGAAAGCA 
TCTGAACTCA TCAGTGAGAG TAAATACCGT CAGCATCCCC AGTCATTCAA GTACACAGCT 
GTGACAGACA CTCCCAGCCT CCTTCATGCA AAACTCAGCA ACCAGATTAC CAATGAGCGC 
CTCTATAAAG CAGCCGGAGA GGATGAGAGG CACCGGTACA CAATGACCCT GGGTCTGCCC 
GAGTTTGTCC GAGCGAAAGC GAACGCAGCC AATCTGAGTG ACGCAAAATA CAAGGAGTCT 
TGGCGTAATC TCCGTGCTCA AGGCTACAAG CTGACAATAG ACGCTCTCCC CTTCCAGGCT 
GCTCGCGTCT CTGGAGATAT AGCCAGTGAT TTTCTCTACA GACATGACTT TGTGAAGGAG 
CGAGGCAGGC TGATCGGGGC CCGGAGTGTG AGTGATGACC CCCGACTCCA GCACTGCTGG 
CGGGTAGGCC AGTTGCAGAG CGAGCTTCAG TACAGGCGGG GAGCGGCGAG CAGCCAAGCC 
CACTACCACC TGCCCATGGA CATGGTGCAC CTGGTCCATG CCAGGAAGGC CCAGGCCCTA 
GCCAGCGACC ACGACTACAG GACACGGTGC CATGAGTTCA CAGCTCTGCC TGAGGACCTG 
AAGATGTCCT GGGCCAAGAG AGCTCACGCC CTGCAGAGTG AGTTTCGCTA CAAGTCAGAC 
CTGATGGGCA TGAAGGGGAC AGGATGGCTG ACGCTGAGTT CGCCGCAGCT AGAGAGTGCA 
AAGAAGGCCG GAGAACTCAT CAGCGAGACC AAATACCGTA AGAAACCAGA TAGTATCAAG 
TTCACCACGG TGGTTGACTC CCCAGACCTG GTTCACGCCA AGAACAGCTA CATGCATTGC 
AATGAGCGCC TGTACAGATT GGGGGATGCG GAATCCCTGC ACAGATATAC GCTGATCCCC 
GACCACCCCG ATTTCACCAG AGCCCGCCTC AACGCGCTGC ATCTGAGTGA TAAAGTCTAC 
AGAAACTCTT GGGAACAGAC CCGGGCTGGT GGCTATGACT TCCGGCTGGA TGCCATCCCA 
TTCCAGACTG CCCGGGCGTC CCGGGAGATC GCCAGTGATT TCCGGTACAA AGAGGCCTTC 
CTGCGGGACC GGGGCCTACA GATTGGTTAC CGCAGCATCC ACGACGACCC CAGGATGAGG 
CATTTCCTCA GCGTCGGCAA ACTCCAGAGT GACAACGAGT ACAAGAAGGA CTTTGCCAAG 
AGTCGGTCCC AGTTCCATAG CCGCCCGGAC CAGCCCGGCT TCCTCCAGGC CAGGAGGAGC 
CAGCAGCTGG CCAGCGACGT GTGCTACCGG CAGCCCCTGC CCCAGCCCAC ATGCGACCCG 
GAGCAGCTAG GCCTGAAGCA CGCACAGAAG GCCCACCAGC TGCAGAGTGA CGTCAAGTAC 
AAATCAGACT TGAACCTGAC CAGAGGCGTT GGCTGGACAC CCCCCGGCTC CTACAAAGTG 
GAAATGGCTC GGCGGGCGGC AGAACTGGCC AAGGCGAGGG GCCTGGGTCT CCGGGGCGCT 
TGGGGGGGGC CAGAGGCTGT GGAGCCCGCA GAGAATCAGA GGGGGGACGT GAACCCAGAC 
GCCACCGAGA TTCTGCACGT CAAAAGGAGG AAGGCTCTGC CGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964424.1
CDS	203..5290
Translation	MNVQACSRCGYGVYPAEKISCLDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRKSPETIHGIDEQDEGERFKSVFHWDMKSRDGTAAPNRQPLMNERAYWNGYGEGNAWCPGAMPDPEIVRMVETRKSLGEQEYTEDYEQQRGKGSFPAMITPAYQRAKKANQLASQVEYKRGHDERISRFTTVADTPELLRAQAGGQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQREMKGMASPAAGAAGTRECMDRYGQQGYLEEYQEHRGKGSFPAMITPAYQNAKKANELASDIKYRQDFNKMKGPAHYHSLPAQDNLVLKRAQSVNKLVSENKYKENYQNHMRGHYEGVGMDRRTLHAMKVGSLASNIAYKADYKHDVVDYNYPATLTPSYQTTMKLVPLKDVNYRQSIDKLKYSSVTNTPQIIQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAELFSEVKYKEGWEKAKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKVMGTTDSRLLHSLQVAKMSSEVEYKKGFEESKTHFHLPMDMVHIKHAKKAQALASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLASEKNYRQRVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYSISKDEPLFLQARVNAANLSEKLYKSSWENQKAKGFELRLDSLGFLAAKANRDLASEVKYKEDYERSRGKLIGAKDAQGDSQMSHSLQMSKLQSELEYKKGFEDTRSQCHVSLDMIHLVHARKAQHLATDIGYKTASHQFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGTGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTQTADLPEVLLAKLNAMNISETRYKESWSKLRDGGYKLRLDAIPFQAAKASGEIISDYKYKEAFEKMKGQMLGSRSLEDDISLAHSVYANSLQSEVNYKKGFELSKAHFHLPLDMVTLVHAKKAQSLASDQDYRHPLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVPCVIPGTLEIEGRKKASELISESKYRQHPQSFKYTAVTDTPSLLHAKLSNQITNERLYKAAGEDERHRYTMTLGLPEFVRAKANAANLSDAKYKESWRNLRAQGYKLTIDALPFQAARVSGDIASDFLYRHDFVKERGRLIGARSVSDDPRLQHCWRVGQLQSELQYRRGAASSQAHYHLPMDMVHLVHARKAQALASDHDYRTRCHEFTALPEDLKMSWAKRAHALQSEFRYKSDLMGMKGTGWLTLSSPQLESAKKAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERLYRLGDAESLHRYTLIPDHPDFTRARLNALHLSDKVYRNSWEQTRAGGYDFRLDAIPFQTARASREIASDFRYKEAFLRDRGLQIGYRSIHDDPRMRHFLSVGKLQSDNEYKKDFAKSRSQFHSRPDQPGFLQARRSQQLASDVCYRQPLPQPTCDPEQLGLKHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARRAAELAKARGLGLRGAWGGPEAVEPAENQRGDVNPDATEILHVKRRKALPL

Target ORF information:

RefSeq Version	XM_014108949.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nebulin-related anchoring protein (NRAP), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108949.1

ORF Insert Sequence:

ATGAATGTCC AGGCTTGCTC TAGGTGTGGG TATGGAGTTT ATCCTGCCGA GAAGATCAGC 
TGTCTAGATC AGATATGGCA TAAAGCATGT TTTCACTGCG AAGTTTGCAA GATGATGCTA 
TCGGTTAATA ACTTCGTGAG TCACCAGAAA AAGCCGTACT GTCACGCCCA CAACCCCAAG 
AACAACACTT TCACAAGTGT CTATCATACT CCCTTAAATC TAAATGTGAG GAAGTCTCCA 
GAAACCATCC ATGGGATTGA TGAGCAAGAT GAGGGCGAAC GGTTTAAATC AGTTTTTCAC 
TGGGACATGA AATCCAGGGA TGGGACAGCT GCACCTAACA GGCAGCCCCT GATGAACGAG 
AGAGCCTATT GGAATGGATA TGGGGAAGGG AATGCTTGGT GCCCAGGAGC TATGCCAGAC 
CCCGAAATCG TAAGGATGGT TGAAACCCGA AAGTCTCTTG GTGAGCAGGA GTATACAGAA 
GACTATGAAC AACAGAGGGG CAAAGGGAGC TTTCCAGCCA TGATCACACC TGCCTATCAA 
AGAGCCAAGA AAGCTAACCA GCTGGCCAGC CAAGTGGAAT ACAAGAGAGG GCATGACGAG 
CGCATCTCCA GGTTTACCAC GGTGGCGGAT ACCCCTGAGC TGCTACGGGC CCAGGCAGGG 
GGACAGCTTC AAAGTGATGT GAGATACACA GAAGACTATG AACAACAGAG AGGAAAAGGC 
AGTTTCCCTG CTATGATCAC GCCTGCTTAT CAGATAGCCA AGAGAGCCAA TGAGCTGGCT 
AGCGATGTGA GGTACCATCA ACAATATCAA AGAGAAATGA AGGGAATGGC TAGTCCAGCT 
GCTGGAGCTG CAGGCACAAG GGAATGCATG GACCGATATG GCCAGCAGGG TTACTTGGAG 
GAATATCAGG AGCACAGAGG AAAAGGCAGC TTCCCCGCCA TGATCACCCC AGCTTATCAG 
AATGCCAAGA AAGCCAACGA ACTCGCTAGT GACATAAAAT ACAGGCAGGA CTTCAATAAG 
ATGAAAGGCC CTGCACATTA TCACTCCCTC CCAGCCCAGG ACAACTTGGT TCTCAAGCGG 
GCCCAGAGCG TGAACAAGCT TGTGAGCGAG AATAAATATA AAGAAAATTA CCAGAACCAC 
ATGAGAGGCC ACTATGAAGG AGTTGGTATG GACAGACGGA CTCTCCATGC TATGAAGGTT 
GGAAGCTTGG CAAGCAATAT TGCCTACAAG GCTGATTATA AACACGATGT TGTGGACTAC 
AACTACCCAG CCACTCTTAC TCCCTCTTAC CAGACAACAA TGAAACTTGT CCCCTTGAAA 
GATGTCAACT ACAGGCAAAG TATCGACAAG TTGAAGTACA GCTCTGTGAC CAACACCCCT 
CAGATTATCC AAGCCAAAAT CAATGCCCAA CAACTGAGTC ATGTGAATTA TCGTGCTGAC 
TATGAGAAAA ATAAGTTGAA CTACACCTTG CCACAGGATG TACCTCAGCT GGTGAAGGCC 
AAAACCAATG CTGAACTATT TAGTGAGGTT AAGTATAAAG AAGGCTGGGA GAAGGCCAAG 
GGGAAAGGAT TTGAGATGAA GCTGGATGCT ATGTCTTTGC TGGCCGCCAA AGCTTCTGGA 
GAGCTTGCTA GCAATATTAA ATATAAAGAA GAGTATGAAA AAACGAAAGG CAAAGTCATG 
GGCACTACAG ACTCGAGGCT TCTGCACTCT CTGCAGGTGG CCAAGATGAG CAGTGAGGTT 
GAATACAAGA AAGGCTTTGA AGAGAGTAAG ACCCACTTTC ACCTACCCAT GGATATGGTG 
CACATCAAGC ATGCTAAGAA GGCCCAAGCT CTGGCCAGTG ACCTGGACTA CAGGAAAAAG 
CTGCATGAAT ACACTGTACT GCCTGAAGAT ATGAAGACTC AGTGGGCCAA GAAAGCCTAT 
GGGCTCCAGA GTGAGCTGCA GTACAAGGCC GACCTAGCAT GGATGAAAGG AGTCGGGTGG 
CTGACGGAGG GGAGTCTCAA TTTGGAACAG GCCAAGAAGG CTGGCCAGCT GGCCAGCGAG 
AAAAACTACA GGCAGAGGGT GGATGAGCTG AAGTTCACCA GTGTGACCGA CAGCTCCCAG 
ATGGAGCACG CCAAGAAGAG CCAGGAGCTG CAGAGCGGGG TGGCCTACAA GGCAGGAAAT 
GAGCAGTCTG TCCATCAGTA CAGCATCAGC AAAGATGAAC CTCTCTTCTT ACAGGCCCGA 
GTCAATGCCG CCAATCTCAG TGAGAAACTG TATAAGAGCA GCTGGGAAAA CCAGAAGGCA 
AAAGGTTTTG AGCTGCGTCT GGACTCCTTG GGCTTCCTGG CAGCCAAAGC CAACAGGGAC 
CTGGCGAGCG AGGTGAAGTA CAAGGAAGAT TACGAGAGGT CCCGAGGTAA GCTCATTGGA 
GCGAAGGATG CCCAGGGGGA TTCACAAATG AGCCACTCGC TGCAAATGTC CAAGCTGCAG 
AGTGAACTCG AGTACAAGAA AGGGTTCGAG GACACCAGAT CCCAGTGCCA CGTCTCCCTG 
GACATGATCC ACCTTGTGCA TGCCCGCAAG GCTCAGCATT TAGCCACAGA CATAGGCTAC 
AAGACGGCAT CTCATCAGTT CACCGCTTTG CCCACCGACA TGAAGGTGGA ATGGGCCAAG 
AAGGCATACG GTTTACAGAG TGATAACCAA TACAGGGCAG ATGTGAAGTG GATGAAAGGC 
ACGGGCTGGG TCGCCACCGG GTCATTAAAT GTGGAGCAGG CGAAGAAGGC AGGAGAACTC 
ATTAGCGAGA AGAAGTACCG TCAGCATCCA GATGCTTTGA AGTTTACCAG TATTAAAGAC 
ACTCCGGAGA TGGTCCAGGC CAGAATTAGT TATACCCAAG CAGTGGATAG GTTATACAGG 
GAACAAGGAG AAAATATAAA GCATCATTAC ACTCAGACAG CCGACCTCCC TGAAGTCCTG 
CTGGCCAAGC TGAATGCTAT GAATATCAGT GAGACGCGTT ATAAGGAATC CTGGAGCAAA 
CTTCGGGACG GTGGCTATAA ACTGAGGTTG GATGCCATTC CGTTCCAGGC AGCAAAGGCT 
TCTGGCGAAA TCATAAGTGA TTACAAATAC AAAGAGGCAT TTGAGAAAAT GAAAGGGCAG 
ATGCTTGGCT CCCGGAGCTT GGAAGACGAT ATCAGCCTTG CACATTCAGT GTATGCGAAC 
TCGCTGCAGA GCGAAGTGAA TTACAAGAAA GGCTTTGAGC TCTCAAAGGC ACACTTCCAT 
CTGCCGTTGG ACATGGTAAC CCTGGTGCAT GCCAAGAAGG CTCAGAGCCT GGCCAGCGAC 
CAGGACTACA GACACCCGCT CCCCCAGTAC ACATCCTTGG CTGAAGACCT GAGGCTCAGC 
TGCGCCAAGA AAGCTCACAA GCTGCAGAGT GAGAATTTGT ACCGCTCAGA CCTGAACTTT 
ATGCGAGGCG TTCCCTGTGT CATTCCGGGC ACATTAGAGA TTGAAGGGAG AAAGAAAGCA 
TCTGAACTCA TCAGTGAGAG TAAATACCGT CAGCATCCCC AGTCATTCAA GTACACAGCT 
GTGACAGACA CTCCCAGCCT CCTTCATGCA AAACTCAGCA ACCAGATTAC CAATGAGCGC 
CTCTATAAAG CAGCCGGAGA GGATGAGAGG CACCGGTACA CAATGACCCT GGGTCTGCCC 
GAGTTTGTCC GAGCGAAAGC GAACGCAGCC AATCTGAGTG ACGCAAAATA CAAGGAGTCT 
TGGCGTAATC TCCGTGCTCA AGGCTACAAG CTGACAATAG ACGCTCTCCC CTTCCAGGCT 
GCTCGCGTCT CTGGAGATAT AGCCAGTGAT TTTCTCTACA GACATGACTT TGTGAAGGAG 
CGAGGCAGGC TGATCGGGGC CCGGAGTGTG AGTGATGACC CCCGACTCCA GCACTGCTGG 
CGGGTAGGCC AGTTGCAGAG CGAGCTTCAG TACAGGCGGG GAGCGGCGAG CAGCCAAGCC 
CACTACCACC TGCCCATGGA CATGGTGCAC CTGGTCCATG CCAGGAAGGC CCAGGCCCTA 
GCCAGCGACC ACGACTACAG GACACGGTGC CATGAGTTCA CAGCTCTGCC TGAGGACCTG 
AAGATGTCCT GGGCCAAGAG AGCTCACGCC CTGCAGAGTG AGTTTCGCTA CAAGTCAGAC 
CTGATGGGCA TGAAGGGGAC AGGATGGCTG ACGCTGAGTT CGCCGCAGCT AGAGAGTGCA 
AAGAAGGCCG GAGAACTCAT CAGCGAGACC AAATACCGTA AGAAACCAGA TAGTATCAAG 
TTCACCACGG TGGTTGACTC CCCAGACCTG GTTCACGCCA AGAACAGCTA CATGCATTGC 
AATGAGCGCC TGTACAGATT GGGGGATGCG GAATCCCTGC ACAGATATAC GCTGATCCCC 
GACCACCCCG ATTTCACCAG AGCCCGCCTC AACGCGCTGC ATCTGAGTGA TAAAGTCTAC 
AGAAACTCTT GGGAACAGAC CCGGGCTGGT GGCTATGACT TCCGGCTGGA TGCCATCCCA 
TTCCAGACTG CCCGGGCGTC CCGGGAGATC GCCAGTGATT TCCGGTACAA AGAGGCCTTC 
CTGCGGGACC GGGGCCTACA GATTGGTTAC CGCAGCATCC ACGACGACCC CAGGATGAGG 
CATTTCCTCA GCGTCGGCAA ACTCCAGAGT GACAACGAGT ACAAGAAGGA CTTTGCCAAG 
AGTCGGTCCC AGTTCCATAG CCGCCCGGAC CAGCCCGGCT TCCTCCAGGC CAGGAGGAGC 
CAGCAGCTGG CCAGCGACGT GTGCTACCGG CAGCCCCTGC CCCAGCCCAC ATGCGACCCG 
GAGCAGCTAG GCCTGAAGCA CGCACAGAAG GCCCACCAGC TGCAGAGTGA CGTCAAGTAC 
AAATCAGACT TGAACCTGAC CAGAGGCGTT GGCTGGACAC CCCCCGGCTC CTACAAAGTG 
GAAATGGCTC GGCGGGCGGC AGAACTGGCC AAGGCGAGGG GCCTGGGTCT CCGGGGCGCT 
TGGGGGGGGC CAGAGGCTGT GGAGCCCGCA GAGAATCAGA GGGGGGACGT GAACCCAGAC 
GCCACCGAGA TTCTGCACGT CAAAAGGAGG AAGGCTCTGC CGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NRAP
Entrez Gene ID	477823
Full Name	nebulin related anchoring protein
General protein information	Preferred Names nebulin related anchoring protein Names nebulin-related-anchoring protein
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Rattus norvegicus (Norway rat)	Nrap	NP_001107215.1
Xenopus tropicalis (tropical clawed frog)	nrap	XP_002939546.2
Bos taurus (cattle)	NRAP	NP_001091541.1
Canis lupus familiaris (dog)	NRAP	XP_851973.1
Pan troglodytes (chimpanzee)	NRAP	XP_521607.4
Mus musculus (house mouse)	Nrap	NP_032759.3
Homo sapiens (human)	NRAP	NP_001248392.1
	NRAP	XP_001090018.2
Gallus gallus (chicken)	NRAP	XP_003641574.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NRAP cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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