GIT2 cDNA ORF clone, Canis lupus familiaris(dog)

The following GIT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GIT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe26376 XM_858931.5
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OCe26366 XM_005636316.3
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe26367 XM_534715.6
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe26368 XM_005636317.3
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe26369 XM_005636318.3
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe26370 XM_858906.5
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe26371 XM_005636319.3
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe26372 XM_005636320.3
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe26373 XM_858808.5
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe26374 XM_858892.5
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe26375 XM_022410811.1
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OCe282644 XM_022410810.1
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OCe43303 XM_014107944.2
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe43304 XM_014107945.2
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe43305 XM_014107946.2
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe43306 XM_014107947.2
Latest version!
Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OCe43307 XM_014107948.1
Latest version!
Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
OCe26368 XM_005636317.2 Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe43304 XM_014107945.1 Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe26374 XM_858892.4 Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe26376 XM_858931.4 Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OCe26371 XM_005636319.2 Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe26372 XM_005636320.2 Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe43305 XM_014107946.1 Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe26367 XM_534715.5 Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe43303 XM_014107944.1 Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe26370 XM_858906.4 Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe26373 XM_858808.4 Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe43306 XM_014107947.1 Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OCe26366 XM_005636316.2 Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe26369 XM_005636318.2 Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe26376
    Clone ID Related Accession (Same CDS sequence) XM_858931.4 , XM_858931.5
    Accession Version XM_858931.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X15
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_858931.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGTTGCTTGG AAAAGATGCT AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_864024.1
    CDS136..1551
    Translation

    Target ORF information:

    RefSeq Version XM_858931.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_858931.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGTTGCTTGG AAAAGATGCT AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26376
    Clone ID Related Accession (Same CDS sequence) XM_858931.4 , XM_858931.5
    Accession Version XM_858931.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_858931.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGTTGCTTGG AAAAGATGCT AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_864024.1
    CDS135..1550
    Translation

    Target ORF information:

    RefSeq Version XM_858931.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_858931.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGTTGCTTGG AAAAGATGCT AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26366
    Clone ID Related Accession (Same CDS sequence) XM_005636316.3 , XM_005636316.2
    Accession Version XM_005636316.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636316.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGCT TCAAACACTC CAGAGTGAAA ATTCGAACCT CAGGAAACAG
    GCCACAACCA ATATATATCA GGTGCAAACT GGTTCTGAGT ACACAGACAC TTCCAACCAC
    TCTTCCTTAA AGCGACGTCC GTCTGCCCGG GGCAGTAGGC CCATGTCCAT GTACGAGACG
    GGATCAGGTC AGAAACCATA TCTCCCAATG GGAGAAGTGA ACCACCCTGA AGAGAGCAGG
    ACAAGACTCC AGCCTTTCCC CACGCACATC GGGAGGAGTG CGCTTGTGAC CTCCTCTTCA
    TCTCTGCCTT CCTTCCCCTC CACACTTTCC TGGTCGAGGG ACGAAAGCGC CCGAAGGGCC
    TCCAGGCTAG AAAAGCAGAA CAGCACGCCT GAAAGTGACT ACGATAATAC ACCCAATGAC
    ACGGACCCAG ATGACACAGG GTCAAGCAGA AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA
    GGCGATGGCT CAGTACCAGA CACAGTGGAA CCCCACACTG CCCCCAGCCC CATCCTCCCC
    AGCACGGAAG ACGTCATCCG GAAAACTGAA CAGATCACCA AAAACATACA GGAGCTCTTA
    AGAGCCGCTC AAGAAAATAA ACATGACAGC TATATCCCCT GCTCAGAAAG GATACATGTA
    GCTGTTACCG AAATGGCAGC ATTATTCCCC AAAAAACCCA AGTCTGACAC GGTGAGGACT
    TCCCTGCGCC TGCTGACATC CAGTGCCTAC CGACTCCAGT CAGAGTGCAA GAAGACCCTC
    CCCGGGGACC CCGGCCCGCC CACGGACATC CAGCTGGTCA CACAGCAGGT CATCCAGTGT
    GCATATGACA TTGCCAAGGC CGCCAAGCAG CTGGTCACCA TCACCACCAA AGAGAACAAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636373.1
    CDS135..2420
    Translation

    Target ORF information:

    RefSeq Version XM_005636316.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636316.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGCT TCAAACACTC CAGAGTGAAA ATTCGAACCT CAGGAAACAG
    GCCACAACCA ATATATATCA GGTGCAAACT GGTTCTGAGT ACACAGACAC TTCCAACCAC
    TCTTCCTTAA AGCGACGTCC GTCTGCCCGG GGCAGTAGGC CCATGTCCAT GTACGAGACG
    GGATCAGGTC AGAAACCATA TCTCCCAATG GGAGAAGTGA ACCACCCTGA AGAGAGCAGG
    ACAAGACTCC AGCCTTTCCC CACGCACATC GGGAGGAGTG CGCTTGTGAC CTCCTCTTCA
    TCTCTGCCTT CCTTCCCCTC CACACTTTCC TGGTCGAGGG ACGAAAGCGC CCGAAGGGCC
    TCCAGGCTAG AAAAGCAGAA CAGCACGCCT GAAAGTGACT ACGATAATAC ACCCAATGAC
    ACGGACCCAG ATGACACAGG GTCAAGCAGA AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA
    GGCGATGGCT CAGTACCAGA CACAGTGGAA CCCCACACTG CCCCCAGCCC CATCCTCCCC
    AGCACGGAAG ACGTCATCCG GAAAACTGAA CAGATCACCA AAAACATACA GGAGCTCTTA
    AGAGCCGCTC AAGAAAATAA ACATGACAGC TATATCCCCT GCTCAGAAAG GATACATGTA
    GCTGTTACCG AAATGGCAGC ATTATTCCCC AAAAAACCCA AGTCTGACAC GGTGAGGACT
    TCCCTGCGCC TGCTGACATC CAGTGCCTAC CGACTCCAGT CAGAGTGCAA GAAGACCCTC
    CCCGGGGACC CCGGCCCGCC CACGGACATC CAGCTGGTCA CACAGCAGGT CATCCAGTGT
    GCATATGACA TTGCCAAGGC CGCCAAGCAG CTGGTCACCA TCACCACCAA AGAGAACAAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26366
    Clone ID Related Accession (Same CDS sequence) XM_005636316.3 , XM_005636316.2
    Accession Version XM_005636316.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGCT TCAAACACTC CAGAGTGAAA ATTCGAACCT CAGGAAACAG
    GCCACAACCA ATATATATCA GGTGCAAACT GGTTCTGAGT ACACAGACAC TTCCAACCAC
    TCTTCCTTAA AGCGACGTCC GTCTGCCCGG GGCAGTAGGC CCATGTCCAT GTACGAGACG
    GGATCAGGTC AGAAACCATA TCTCCCAATG GGAGAAGTGA ACCACCCTGA AGAGAGCAGG
    ACAAGACTCC AGCCTTTCCC CACGCACATC GGGAGGAGTG CGCTTGTGAC CTCCTCTTCA
    TCTCTGCCTT CCTTCCCCTC CACACTTTCC TGGTCGAGGG ACGAAAGCGC CCGAAGGGCC
    TCCAGGCTAG AAAAGCAGAA CAGCACGCCT GAAAGTGACT ACGATAATAC ACCCAATGAC
    ACGGACCCAG ATGACACAGG GTCAAGCAGA AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA
    GGCGATGGCT CAGTACCAGA CACAGTGGAA CCCCACACTG CCCCCAGCCC CATCCTCCCC
    AGCACGGAAG ACGTCATCCG GAAAACTGAA CAGATCACCA AAAACATACA GGAGCTCTTA
    AGAGCCGCTC AAGAAAATAA ACATGACAGC TATATCCCCT GCTCAGAAAG GATACATGTA
    GCTGTTACCG AAATGGCAGC ATTATTCCCC AAAAAACCCA AGTCTGACAC GGTGAGGACT
    TCCCTGCGCC TGCTGACATC CAGTGCCTAC CGACTCCAGT CAGAGTGCAA GAAGACCCTC
    CCCGGGGACC CCGGCCCGCC CACGGACATC CAGCTGGTCA CACAGCAGGT CATCCAGTGT
    GCATATGACA TTGCCAAGGC CGCCAAGCAG CTGGTCACCA TCACCACCAA AGAGAACAAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636373.1
    CDS136..2421
    Translation

    Target ORF information:

    RefSeq Version XM_005636316.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636316.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGCT TCAAACACTC CAGAGTGAAA ATTCGAACCT CAGGAAACAG
    GCCACAACCA ATATATATCA GGTGCAAACT GGTTCTGAGT ACACAGACAC TTCCAACCAC
    TCTTCCTTAA AGCGACGTCC GTCTGCCCGG GGCAGTAGGC CCATGTCCAT GTACGAGACG
    GGATCAGGTC AGAAACCATA TCTCCCAATG GGAGAAGTGA ACCACCCTGA AGAGAGCAGG
    ACAAGACTCC AGCCTTTCCC CACGCACATC GGGAGGAGTG CGCTTGTGAC CTCCTCTTCA
    TCTCTGCCTT CCTTCCCCTC CACACTTTCC TGGTCGAGGG ACGAAAGCGC CCGAAGGGCC
    TCCAGGCTAG AAAAGCAGAA CAGCACGCCT GAAAGTGACT ACGATAATAC ACCCAATGAC
    ACGGACCCAG ATGACACAGG GTCAAGCAGA AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA
    GGCGATGGCT CAGTACCAGA CACAGTGGAA CCCCACACTG CCCCCAGCCC CATCCTCCCC
    AGCACGGAAG ACGTCATCCG GAAAACTGAA CAGATCACCA AAAACATACA GGAGCTCTTA
    AGAGCCGCTC AAGAAAATAA ACATGACAGC TATATCCCCT GCTCAGAAAG GATACATGTA
    GCTGTTACCG AAATGGCAGC ATTATTCCCC AAAAAACCCA AGTCTGACAC GGTGAGGACT
    TCCCTGCGCC TGCTGACATC CAGTGCCTAC CGACTCCAGT CAGAGTGCAA GAAGACCCTC
    CCCGGGGACC CCGGCCCGCC CACGGACATC CAGCTGGTCA CACAGCAGGT CATCCAGTGT
    GCATATGACA TTGCCAAGGC CGCCAAGCAG CTGGTCACCA TCACCACCAA AGAGAACAAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26367
    Clone ID Related Accession (Same CDS sequence) XM_534715.5 , XM_534715.6
    Accession Version XM_534715.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_534715.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACGGGATCA
    GGTCAGAAAC CATATCTCCC AATGGGAGAA GTGAACCACC CTGAAGAGAG CAGGACAAGA
    CTCCAGCCTT TCCCCACGCA CATCGGGAGG AGTGCGCTTG TGACCTCCTC TTCATCTCTG
    CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCCTCCAGG
    CTAGAAAAGC AGAACAGCAC GCCTGAAAGT GACTACGATA ATACACCCAA TGACACGGAC
    CCAGATGACA CAGGGTCAAG CAGAAAGGGA AGGCAGAGGA GTATGGTGTG GCAAGGCGAT
    GGCTCAGTAC CAGACACAGT GGAACCCCAC ACTGCCCCCA GCCCCATCCT CCCCAGCACG
    GAAGACGTCA TCCGGAAAAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCC
    GCTCAAGAAA ATAAACATGA CAGCTATATC CCCTGCTCAG AAAGGATACA TGTAGCTGTT
    ACCGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ACACGGTGAG GACTTCCCTG
    CGCCTGCTGA CATCCAGTGC CTACCGACTC CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG
    GACCCCGGCC CGCCCACGGA CATCCAGCTG GTCACACAGC AGGTCATCCA GTGTGCATAT
    GACATTGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_534715.2
    CDS136..2415
    Translation

    Target ORF information:

    RefSeq Version XM_534715.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_534715.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACGGGATCA
    GGTCAGAAAC CATATCTCCC AATGGGAGAA GTGAACCACC CTGAAGAGAG CAGGACAAGA
    CTCCAGCCTT TCCCCACGCA CATCGGGAGG AGTGCGCTTG TGACCTCCTC TTCATCTCTG
    CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCCTCCAGG
    CTAGAAAAGC AGAACAGCAC GCCTGAAAGT GACTACGATA ATACACCCAA TGACACGGAC
    CCAGATGACA CAGGGTCAAG CAGAAAGGGA AGGCAGAGGA GTATGGTGTG GCAAGGCGAT
    GGCTCAGTAC CAGACACAGT GGAACCCCAC ACTGCCCCCA GCCCCATCCT CCCCAGCACG
    GAAGACGTCA TCCGGAAAAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCC
    GCTCAAGAAA ATAAACATGA CAGCTATATC CCCTGCTCAG AAAGGATACA TGTAGCTGTT
    ACCGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ACACGGTGAG GACTTCCCTG
    CGCCTGCTGA CATCCAGTGC CTACCGACTC CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG
    GACCCCGGCC CGCCCACGGA CATCCAGCTG GTCACACAGC AGGTCATCCA GTGTGCATAT
    GACATTGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26367
    Clone ID Related Accession (Same CDS sequence) XM_534715.5 , XM_534715.6
    Accession Version XM_534715.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_534715.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACGGGATCA
    GGTCAGAAAC CATATCTCCC AATGGGAGAA GTGAACCACC CTGAAGAGAG CAGGACAAGA
    CTCCAGCCTT TCCCCACGCA CATCGGGAGG AGTGCGCTTG TGACCTCCTC TTCATCTCTG
    CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCCTCCAGG
    CTAGAAAAGC AGAACAGCAC GCCTGAAAGT GACTACGATA ATACACCCAA TGACACGGAC
    CCAGATGACA CAGGGTCAAG CAGAAAGGGA AGGCAGAGGA GTATGGTGTG GCAAGGCGAT
    GGCTCAGTAC CAGACACAGT GGAACCCCAC ACTGCCCCCA GCCCCATCCT CCCCAGCACG
    GAAGACGTCA TCCGGAAAAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCC
    GCTCAAGAAA ATAAACATGA CAGCTATATC CCCTGCTCAG AAAGGATACA TGTAGCTGTT
    ACCGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ACACGGTGAG GACTTCCCTG
    CGCCTGCTGA CATCCAGTGC CTACCGACTC CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG
    GACCCCGGCC CGCCCACGGA CATCCAGCTG GTCACACAGC AGGTCATCCA GTGTGCATAT
    GACATTGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_534715.2
    CDS135..2414
    Translation

    Target ORF information:

    RefSeq Version XM_534715.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_534715.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACGGGATCA
    GGTCAGAAAC CATATCTCCC AATGGGAGAA GTGAACCACC CTGAAGAGAG CAGGACAAGA
    CTCCAGCCTT TCCCCACGCA CATCGGGAGG AGTGCGCTTG TGACCTCCTC TTCATCTCTG
    CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCCTCCAGG
    CTAGAAAAGC AGAACAGCAC GCCTGAAAGT GACTACGATA ATACACCCAA TGACACGGAC
    CCAGATGACA CAGGGTCAAG CAGAAAGGGA AGGCAGAGGA GTATGGTGTG GCAAGGCGAT
    GGCTCAGTAC CAGACACAGT GGAACCCCAC ACTGCCCCCA GCCCCATCCT CCCCAGCACG
    GAAGACGTCA TCCGGAAAAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCC
    GCTCAAGAAA ATAAACATGA CAGCTATATC CCCTGCTCAG AAAGGATACA TGTAGCTGTT
    ACCGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ACACGGTGAG GACTTCCCTG
    CGCCTGCTGA CATCCAGTGC CTACCGACTC CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG
    GACCCCGGCC CGCCCACGGA CATCCAGCTG GTCACACAGC AGGTCATCCA GTGTGCATAT
    GACATTGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26368
    Clone ID Related Accession (Same CDS sequence) XM_005636317.3 , XM_005636317.2
    Accession Version XM_005636317.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636317.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTCCAA TCCCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAAAACCACA GCACCCTGGT AACCGAGACA ACTGTCGTCC CCTTCCTTCC GGTCAATCCT
    GAGTACTCTT CAACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACAC TGGTCATCGA CATTCTCAGT GACGCCAAGA GGAGACAGCA AGGCAGTCCT
    CTTTCTGGTT CTAAAGACAA TGTGGAGCTG ATACTGAAAA CGATCAGTAA CCAGCACAGC
    ATTGAGAGTC AAGACAATGA CCAGCCAGAC TATGACAGTG TGGCCTCAGA CGAAGACACG
    GATCTGGAAG CTACTGCAAG CAAAGCAAAC AGGCAGAAGA GTCTAGATTC AGATTTATCA
    GATGGACCAG TCACTGTGCA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG
    GCCAAGATAC AGCAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG
    CAGAAAAAGC TTCAAACACT CCAGAGTGAA AATTCGAACC TCAGGAAACA GGCCACAACC
    AATATATATC AGGTGCAAAC TGGTTCTGAG TACACAGACA CTTCCAACCA CTCTTCCTTA
    AAGCGACGTC CGTCTGCCCG GGGCAGTAGG CCCATGTCCA TGTACGAGAC GGGATCAGGT
    CAGAAACCAT ATCTCCCAAT GGGAGAAGTG AACCACCCTG AAGAGAGCAG GACAAGACTC
    CAGCCTTTCC CCACGCACAT CGGGAGGAGT GCGCTTGTGA CCTCCTCTTC ATCTCTGCCT
    TCCTTCCCCT CCACACTTTC CTGGTCGAGG GACGAAAGCG CCCGAAGGGC CTCCAGGCTA
    GAAAAGCAGA ACAGCACGCC TGAAAGTGAC TACGATAATA CACCCAATGA CACGGACCCA
    GATGACACAG GGTCAAGCAG AAAGGGAAGG CAGAGGAGTA TGGTGTGGCA AGGCGATGGC
    TCAGTACCAG ACACAGTGGA ACCCCACACT GCCCCCAGCC CCATCCTCCC CAGCACGGAA
    GACGTCATCC GGAAAACTGA ACAGATCACC AAAAACATAC AGGAGCTCTT AAGAGCCGCT
    CAAGAAAATA AACATGACAG CTATATCCCC TGCTCAGAAA GGATACATGT AGCTGTTACC
    GAAATGGCAG CATTATTCCC CAAAAAACCC AAGTCTGACA CGGTGAGGAC TTCCCTGCGC
    CTGCTGACAT CCAGTGCCTA CCGACTCCAG TCAGAGTGCA AGAAGACCCT CCCCGGGGAC
    CCCGGCCCGC CCACGGACAT CCAGCTGGTC ACACAGCAGG TCATCCAGTG TGCATATGAC
    ATTGCCAAGG CCGCCAAGCA GCTGGTCACC ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636374.1
    CDS135..2411
    Translation

    Target ORF information:

    RefSeq Version XM_005636317.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636317.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTCCAA TCCCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAAAACCACA GCACCCTGGT AACCGAGACA ACTGTCGTCC CCTTCCTTCC GGTCAATCCT
    GAGTACTCTT CAACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACAC TGGTCATCGA CATTCTCAGT GACGCCAAGA GGAGACAGCA AGGCAGTCCT
    CTTTCTGGTT CTAAAGACAA TGTGGAGCTG ATACTGAAAA CGATCAGTAA CCAGCACAGC
    ATTGAGAGTC AAGACAATGA CCAGCCAGAC TATGACAGTG TGGCCTCAGA CGAAGACACG
    GATCTGGAAG CTACTGCAAG CAAAGCAAAC AGGCAGAAGA GTCTAGATTC AGATTTATCA
    GATGGACCAG TCACTGTGCA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG
    GCCAAGATAC AGCAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG
    CAGAAAAAGC TTCAAACACT CCAGAGTGAA AATTCGAACC TCAGGAAACA GGCCACAACC
    AATATATATC AGGTGCAAAC TGGTTCTGAG TACACAGACA CTTCCAACCA CTCTTCCTTA
    AAGCGACGTC CGTCTGCCCG GGGCAGTAGG CCCATGTCCA TGTACGAGAC GGGATCAGGT
    CAGAAACCAT ATCTCCCAAT GGGAGAAGTG AACCACCCTG AAGAGAGCAG GACAAGACTC
    CAGCCTTTCC CCACGCACAT CGGGAGGAGT GCGCTTGTGA CCTCCTCTTC ATCTCTGCCT
    TCCTTCCCCT CCACACTTTC CTGGTCGAGG GACGAAAGCG CCCGAAGGGC CTCCAGGCTA
    GAAAAGCAGA ACAGCACGCC TGAAAGTGAC TACGATAATA CACCCAATGA CACGGACCCA
    GATGACACAG GGTCAAGCAG AAAGGGAAGG CAGAGGAGTA TGGTGTGGCA AGGCGATGGC
    TCAGTACCAG ACACAGTGGA ACCCCACACT GCCCCCAGCC CCATCCTCCC CAGCACGGAA
    GACGTCATCC GGAAAACTGA ACAGATCACC AAAAACATAC AGGAGCTCTT AAGAGCCGCT
    CAAGAAAATA AACATGACAG CTATATCCCC TGCTCAGAAA GGATACATGT AGCTGTTACC
    GAAATGGCAG CATTATTCCC CAAAAAACCC AAGTCTGACA CGGTGAGGAC TTCCCTGCGC
    CTGCTGACAT CCAGTGCCTA CCGACTCCAG TCAGAGTGCA AGAAGACCCT CCCCGGGGAC
    CCCGGCCCGC CCACGGACAT CCAGCTGGTC ACACAGCAGG TCATCCAGTG TGCATATGAC
    ATTGCCAAGG CCGCCAAGCA GCTGGTCACC ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26368
    Clone ID Related Accession (Same CDS sequence) XM_005636317.3 , XM_005636317.2
    Accession Version XM_005636317.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTCCAA TCCCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAAAACCACA GCACCCTGGT AACCGAGACA ACTGTCGTCC CCTTCCTTCC GGTCAATCCT
    GAGTACTCTT CAACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACAC TGGTCATCGA CATTCTCAGT GACGCCAAGA GGAGACAGCA AGGCAGTCCT
    CTTTCTGGTT CTAAAGACAA TGTGGAGCTG ATACTGAAAA CGATCAGTAA CCAGCACAGC
    ATTGAGAGTC AAGACAATGA CCAGCCAGAC TATGACAGTG TGGCCTCAGA CGAAGACACG
    GATCTGGAAG CTACTGCAAG CAAAGCAAAC AGGCAGAAGA GTCTAGATTC AGATTTATCA
    GATGGACCAG TCACTGTGCA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG
    GCCAAGATAC AGCAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG
    CAGAAAAAGC TTCAAACACT CCAGAGTGAA AATTCGAACC TCAGGAAACA GGCCACAACC
    AATATATATC AGGTGCAAAC TGGTTCTGAG TACACAGACA CTTCCAACCA CTCTTCCTTA
    AAGCGACGTC CGTCTGCCCG GGGCAGTAGG CCCATGTCCA TGTACGAGAC GGGATCAGGT
    CAGAAACCAT ATCTCCCAAT GGGAGAAGTG AACCACCCTG AAGAGAGCAG GACAAGACTC
    CAGCCTTTCC CCACGCACAT CGGGAGGAGT GCGCTTGTGA CCTCCTCTTC ATCTCTGCCT
    TCCTTCCCCT CCACACTTTC CTGGTCGAGG GACGAAAGCG CCCGAAGGGC CTCCAGGCTA
    GAAAAGCAGA ACAGCACGCC TGAAAGTGAC TACGATAATA CACCCAATGA CACGGACCCA
    GATGACACAG GGTCAAGCAG AAAGGGAAGG CAGAGGAGTA TGGTGTGGCA AGGCGATGGC
    TCAGTACCAG ACACAGTGGA ACCCCACACT GCCCCCAGCC CCATCCTCCC CAGCACGGAA
    GACGTCATCC GGAAAACTGA ACAGATCACC AAAAACATAC AGGAGCTCTT AAGAGCCGCT
    CAAGAAAATA AACATGACAG CTATATCCCC TGCTCAGAAA GGATACATGT AGCTGTTACC
    GAAATGGCAG CATTATTCCC CAAAAAACCC AAGTCTGACA CGGTGAGGAC TTCCCTGCGC
    CTGCTGACAT CCAGTGCCTA CCGACTCCAG TCAGAGTGCA AGAAGACCCT CCCCGGGGAC
    CCCGGCCCGC CCACGGACAT CCAGCTGGTC ACACAGCAGG TCATCCAGTG TGCATATGAC
    ATTGCCAAGG CCGCCAAGCA GCTGGTCACC ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636374.1
    CDS136..2412
    Translation

    Target ORF information:

    RefSeq Version XM_005636317.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636317.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTCCAA TCCCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAAAACCACA GCACCCTGGT AACCGAGACA ACTGTCGTCC CCTTCCTTCC GGTCAATCCT
    GAGTACTCTT CAACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACAC TGGTCATCGA CATTCTCAGT GACGCCAAGA GGAGACAGCA AGGCAGTCCT
    CTTTCTGGTT CTAAAGACAA TGTGGAGCTG ATACTGAAAA CGATCAGTAA CCAGCACAGC
    ATTGAGAGTC AAGACAATGA CCAGCCAGAC TATGACAGTG TGGCCTCAGA CGAAGACACG
    GATCTGGAAG CTACTGCAAG CAAAGCAAAC AGGCAGAAGA GTCTAGATTC AGATTTATCA
    GATGGACCAG TCACTGTGCA GGAATTTATG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG
    GCCAAGATAC AGCAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG
    CAGAAAAAGC TTCAAACACT CCAGAGTGAA AATTCGAACC TCAGGAAACA GGCCACAACC
    AATATATATC AGGTGCAAAC TGGTTCTGAG TACACAGACA CTTCCAACCA CTCTTCCTTA
    AAGCGACGTC CGTCTGCCCG GGGCAGTAGG CCCATGTCCA TGTACGAGAC GGGATCAGGT
    CAGAAACCAT ATCTCCCAAT GGGAGAAGTG AACCACCCTG AAGAGAGCAG GACAAGACTC
    CAGCCTTTCC CCACGCACAT CGGGAGGAGT GCGCTTGTGA CCTCCTCTTC ATCTCTGCCT
    TCCTTCCCCT CCACACTTTC CTGGTCGAGG GACGAAAGCG CCCGAAGGGC CTCCAGGCTA
    GAAAAGCAGA ACAGCACGCC TGAAAGTGAC TACGATAATA CACCCAATGA CACGGACCCA
    GATGACACAG GGTCAAGCAG AAAGGGAAGG CAGAGGAGTA TGGTGTGGCA AGGCGATGGC
    TCAGTACCAG ACACAGTGGA ACCCCACACT GCCCCCAGCC CCATCCTCCC CAGCACGGAA
    GACGTCATCC GGAAAACTGA ACAGATCACC AAAAACATAC AGGAGCTCTT AAGAGCCGCT
    CAAGAAAATA AACATGACAG CTATATCCCC TGCTCAGAAA GGATACATGT AGCTGTTACC
    GAAATGGCAG CATTATTCCC CAAAAAACCC AAGTCTGACA CGGTGAGGAC TTCCCTGCGC
    CTGCTGACAT CCAGTGCCTA CCGACTCCAG TCAGAGTGCA AGAAGACCCT CCCCGGGGAC
    CCCGGCCCGC CCACGGACAT CCAGCTGGTC ACACAGCAGG TCATCCAGTG TGCATATGAC
    ATTGCCAAGG CCGCCAAGCA GCTGGTCACC ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26369
    Clone ID Related Accession (Same CDS sequence) XM_005636318.3 , XM_005636318.2
    Accession Version XM_005636318.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636318.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGCTTCAAA CACTCCAGAG TGAAAATTCG
    AACCTCAGGA AACAGGCCAC AACCAATATA TATCAGGTGC AAACTGGTTC TGAGTACACA
    GACACTTCCA ACCACTCTTC CTTAAAGCGA CGTCCGTCTG CCCGGGGCAG TAGGCCCATG
    TCCATGTACG AGACGGGATC AGGTCAGAAA CCATATCTCC CAATGGGAGA AGTGAACCAC
    CCTGAAGAGA GCAGGACAAG ACTCCAGCCT TTCCCCACGC ACATCGGGAG GAGTGCGCTT
    GTGACCTCCT CTTCATCTCT GCCTTCCTTC CCCTCCACAC TTTCCTGGTC GAGGGACGAA
    AGCGCCCGAA GGGCCTCCAG GCTAGAAAAG CAGAACAGCA CGCCTGAAAG TGACTACGAT
    AATACACCCA ATGACACGGA CCCAGATGAC ACAGGGTCAA GCAGAAAGGG AAGGCAGAGG
    AGTATGGTGT GGCAAGGCGA TGGCTCAGTA CCAGACACAG TGGAACCCCA CACTGCCCCC
    AGCCCCATCC TCCCCAGCAC GGAAGACGTC ATCCGGAAAA CTGAACAGAT CACCAAAAAC
    ATACAGGAGC TCTTAAGAGC CGCTCAAGAA AATAAACATG ACAGCTATAT CCCCTGCTCA
    GAAAGGATAC ATGTAGCTGT TACCGAAATG GCAGCATTAT TCCCCAAAAA ACCCAAGTCT
    GACACGGTGA GGACTTCCCT GCGCCTGCTG ACATCCAGTG CCTACCGACT CCAGTCAGAG
    TGCAAGAAGA CCCTCCCCGG GGACCCCGGC CCGCCCACGG ACATCCAGCT GGTCACACAG
    CAGGTCATCC AGTGTGCATA TGACATTGCC AAGGCCGCCA AGCAGCTGGT CACCATCACC
    ACCAAAGAGA ACAACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636375.1
    CDS135..2375
    Translation

    Target ORF information:

    RefSeq Version XM_005636318.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636318.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGCTTCAAA CACTCCAGAG TGAAAATTCG
    AACCTCAGGA AACAGGCCAC AACCAATATA TATCAGGTGC AAACTGGTTC TGAGTACACA
    GACACTTCCA ACCACTCTTC CTTAAAGCGA CGTCCGTCTG CCCGGGGCAG TAGGCCCATG
    TCCATGTACG AGACGGGATC AGGTCAGAAA CCATATCTCC CAATGGGAGA AGTGAACCAC
    CCTGAAGAGA GCAGGACAAG ACTCCAGCCT TTCCCCACGC ACATCGGGAG GAGTGCGCTT
    GTGACCTCCT CTTCATCTCT GCCTTCCTTC CCCTCCACAC TTTCCTGGTC GAGGGACGAA
    AGCGCCCGAA GGGCCTCCAG GCTAGAAAAG CAGAACAGCA CGCCTGAAAG TGACTACGAT
    AATACACCCA ATGACACGGA CCCAGATGAC ACAGGGTCAA GCAGAAAGGG AAGGCAGAGG
    AGTATGGTGT GGCAAGGCGA TGGCTCAGTA CCAGACACAG TGGAACCCCA CACTGCCCCC
    AGCCCCATCC TCCCCAGCAC GGAAGACGTC ATCCGGAAAA CTGAACAGAT CACCAAAAAC
    ATACAGGAGC TCTTAAGAGC CGCTCAAGAA AATAAACATG ACAGCTATAT CCCCTGCTCA
    GAAAGGATAC ATGTAGCTGT TACCGAAATG GCAGCATTAT TCCCCAAAAA ACCCAAGTCT
    GACACGGTGA GGACTTCCCT GCGCCTGCTG ACATCCAGTG CCTACCGACT CCAGTCAGAG
    TGCAAGAAGA CCCTCCCCGG GGACCCCGGC CCGCCCACGG ACATCCAGCT GGTCACACAG
    CAGGTCATCC AGTGTGCATA TGACATTGCC AAGGCCGCCA AGCAGCTGGT CACCATCACC
    ACCAAAGAGA ACAACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26369
    Clone ID Related Accession (Same CDS sequence) XM_005636318.3 , XM_005636318.2
    Accession Version XM_005636318.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGCTTCAAA CACTCCAGAG TGAAAATTCG
    AACCTCAGGA AACAGGCCAC AACCAATATA TATCAGGTGC AAACTGGTTC TGAGTACACA
    GACACTTCCA ACCACTCTTC CTTAAAGCGA CGTCCGTCTG CCCGGGGCAG TAGGCCCATG
    TCCATGTACG AGACGGGATC AGGTCAGAAA CCATATCTCC CAATGGGAGA AGTGAACCAC
    CCTGAAGAGA GCAGGACAAG ACTCCAGCCT TTCCCCACGC ACATCGGGAG GAGTGCGCTT
    GTGACCTCCT CTTCATCTCT GCCTTCCTTC CCCTCCACAC TTTCCTGGTC GAGGGACGAA
    AGCGCCCGAA GGGCCTCCAG GCTAGAAAAG CAGAACAGCA CGCCTGAAAG TGACTACGAT
    AATACACCCA ATGACACGGA CCCAGATGAC ACAGGGTCAA GCAGAAAGGG AAGGCAGAGG
    AGTATGGTGT GGCAAGGCGA TGGCTCAGTA CCAGACACAG TGGAACCCCA CACTGCCCCC
    AGCCCCATCC TCCCCAGCAC GGAAGACGTC ATCCGGAAAA CTGAACAGAT CACCAAAAAC
    ATACAGGAGC TCTTAAGAGC CGCTCAAGAA AATAAACATG ACAGCTATAT CCCCTGCTCA
    GAAAGGATAC ATGTAGCTGT TACCGAAATG GCAGCATTAT TCCCCAAAAA ACCCAAGTCT
    GACACGGTGA GGACTTCCCT GCGCCTGCTG ACATCCAGTG CCTACCGACT CCAGTCAGAG
    TGCAAGAAGA CCCTCCCCGG GGACCCCGGC CCGCCCACGG ACATCCAGCT GGTCACACAG
    CAGGTCATCC AGTGTGCATA TGACATTGCC AAGGCCGCCA AGCAGCTGGT CACCATCACC
    ACCAAAGAGA ACAACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636375.1
    CDS136..2376
    Translation

    Target ORF information:

    RefSeq Version XM_005636318.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636318.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGCTTCAAA CACTCCAGAG TGAAAATTCG
    AACCTCAGGA AACAGGCCAC AACCAATATA TATCAGGTGC AAACTGGTTC TGAGTACACA
    GACACTTCCA ACCACTCTTC CTTAAAGCGA CGTCCGTCTG CCCGGGGCAG TAGGCCCATG
    TCCATGTACG AGACGGGATC AGGTCAGAAA CCATATCTCC CAATGGGAGA AGTGAACCAC
    CCTGAAGAGA GCAGGACAAG ACTCCAGCCT TTCCCCACGC ACATCGGGAG GAGTGCGCTT
    GTGACCTCCT CTTCATCTCT GCCTTCCTTC CCCTCCACAC TTTCCTGGTC GAGGGACGAA
    AGCGCCCGAA GGGCCTCCAG GCTAGAAAAG CAGAACAGCA CGCCTGAAAG TGACTACGAT
    AATACACCCA ATGACACGGA CCCAGATGAC ACAGGGTCAA GCAGAAAGGG AAGGCAGAGG
    AGTATGGTGT GGCAAGGCGA TGGCTCAGTA CCAGACACAG TGGAACCCCA CACTGCCCCC
    AGCCCCATCC TCCCCAGCAC GGAAGACGTC ATCCGGAAAA CTGAACAGAT CACCAAAAAC
    ATACAGGAGC TCTTAAGAGC CGCTCAAGAA AATAAACATG ACAGCTATAT CCCCTGCTCA
    GAAAGGATAC ATGTAGCTGT TACCGAAATG GCAGCATTAT TCCCCAAAAA ACCCAAGTCT
    GACACGGTGA GGACTTCCCT GCGCCTGCTG ACATCCAGTG CCTACCGACT CCAGTCAGAG
    TGCAAGAAGA CCCTCCCCGG GGACCCCGGC CCGCCCACGG ACATCCAGCT GGTCACACAG
    CAGGTCATCC AGTGTGCATA TGACATTGCC AAGGCCGCCA AGCAGCTGGT CACCATCACC
    ACCAAAGAGA ACAACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26370
    Clone ID Related Accession (Same CDS sequence) XM_858906.4 , XM_858906.5
    Accession Version XM_858906.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_858906.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACGGGATCA
    GGTCAGAAAC CATATCTCCC AATGGGAGAA GTGAACCACC CTGAAGAGAG CAGGACAAGA
    CTCCAGCCTT TCCCCACGCA CGCCTCCAGG CTAGAAAAGC AGAACAGCAC GCCTGAAAGT
    GACTACGATA ATACACCCAA TGACACGGAC CCAGATGACA CAGGGTCAAG CAGAAAGGGA
    AGGCAGAGGA GTATGGTGTG GCAAGGCGAT GGCTCAGTAC CAGACACAGT GGAACCCCAC
    ACTGCCCCCA GCCCCATCCT CCCCAGCACG GAAGACGTCA TCCGGAAAAC TGAACAGATC
    ACCAAAAACA TACAGGAGCT CTTAAGAGCC GCTCAAGAAA ATAAACATGA CAGCTATATC
    CCCTGCTCAG AAAGGATACA TGTAGCTGTT ACCGAAATGG CAGCATTATT CCCCAAAAAA
    CCCAAGTCTG ACACGGTGAG GACTTCCCTG CGCCTGCTGA CATCCAGTGC CTACCGACTC
    CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG GACCCCGGCC CGCCCACGGA CATCCAGCTG
    GTCACACAGC AGGTCATCCA GTGTGCATAT GACATTGCCA AGGCCGCCAA GCAGCTGGTC
    ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_863999.1
    CDS136..2325
    Translation

    Target ORF information:

    RefSeq Version XM_858906.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_858906.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACGGGATCA
    GGTCAGAAAC CATATCTCCC AATGGGAGAA GTGAACCACC CTGAAGAGAG CAGGACAAGA
    CTCCAGCCTT TCCCCACGCA CGCCTCCAGG CTAGAAAAGC AGAACAGCAC GCCTGAAAGT
    GACTACGATA ATACACCCAA TGACACGGAC CCAGATGACA CAGGGTCAAG CAGAAAGGGA
    AGGCAGAGGA GTATGGTGTG GCAAGGCGAT GGCTCAGTAC CAGACACAGT GGAACCCCAC
    ACTGCCCCCA GCCCCATCCT CCCCAGCACG GAAGACGTCA TCCGGAAAAC TGAACAGATC
    ACCAAAAACA TACAGGAGCT CTTAAGAGCC GCTCAAGAAA ATAAACATGA CAGCTATATC
    CCCTGCTCAG AAAGGATACA TGTAGCTGTT ACCGAAATGG CAGCATTATT CCCCAAAAAA
    CCCAAGTCTG ACACGGTGAG GACTTCCCTG CGCCTGCTGA CATCCAGTGC CTACCGACTC
    CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG GACCCCGGCC CGCCCACGGA CATCCAGCTG
    GTCACACAGC AGGTCATCCA GTGTGCATAT GACATTGCCA AGGCCGCCAA GCAGCTGGTC
    ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26370
    Clone ID Related Accession (Same CDS sequence) XM_858906.4 , XM_858906.5
    Accession Version XM_858906.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_858906.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACGGGATCA
    GGTCAGAAAC CATATCTCCC AATGGGAGAA GTGAACCACC CTGAAGAGAG CAGGACAAGA
    CTCCAGCCTT TCCCCACGCA CGCCTCCAGG CTAGAAAAGC AGAACAGCAC GCCTGAAAGT
    GACTACGATA ATACACCCAA TGACACGGAC CCAGATGACA CAGGGTCAAG CAGAAAGGGA
    AGGCAGAGGA GTATGGTGTG GCAAGGCGAT GGCTCAGTAC CAGACACAGT GGAACCCCAC
    ACTGCCCCCA GCCCCATCCT CCCCAGCACG GAAGACGTCA TCCGGAAAAC TGAACAGATC
    ACCAAAAACA TACAGGAGCT CTTAAGAGCC GCTCAAGAAA ATAAACATGA CAGCTATATC
    CCCTGCTCAG AAAGGATACA TGTAGCTGTT ACCGAAATGG CAGCATTATT CCCCAAAAAA
    CCCAAGTCTG ACACGGTGAG GACTTCCCTG CGCCTGCTGA CATCCAGTGC CTACCGACTC
    CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG GACCCCGGCC CGCCCACGGA CATCCAGCTG
    GTCACACAGC AGGTCATCCA GTGTGCATAT GACATTGCCA AGGCCGCCAA GCAGCTGGTC
    ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_863999.1
    CDS135..2324
    Translation

    Target ORF information:

    RefSeq Version XM_858906.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_858906.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACGGGATCA
    GGTCAGAAAC CATATCTCCC AATGGGAGAA GTGAACCACC CTGAAGAGAG CAGGACAAGA
    CTCCAGCCTT TCCCCACGCA CGCCTCCAGG CTAGAAAAGC AGAACAGCAC GCCTGAAAGT
    GACTACGATA ATACACCCAA TGACACGGAC CCAGATGACA CAGGGTCAAG CAGAAAGGGA
    AGGCAGAGGA GTATGGTGTG GCAAGGCGAT GGCTCAGTAC CAGACACAGT GGAACCCCAC
    ACTGCCCCCA GCCCCATCCT CCCCAGCACG GAAGACGTCA TCCGGAAAAC TGAACAGATC
    ACCAAAAACA TACAGGAGCT CTTAAGAGCC GCTCAAGAAA ATAAACATGA CAGCTATATC
    CCCTGCTCAG AAAGGATACA TGTAGCTGTT ACCGAAATGG CAGCATTATT CCCCAAAAAA
    CCCAAGTCTG ACACGGTGAG GACTTCCCTG CGCCTGCTGA CATCCAGTGC CTACCGACTC
    CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG GACCCCGGCC CGCCCACGGA CATCCAGCTG
    GTCACACAGC AGGTCATCCA GTGTGCATAT GACATTGCCA AGGCCGCCAA GCAGCTGGTC
    ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26371
    Clone ID Related Accession (Same CDS sequence) XM_005636319.2 , XM_005636319.3
    Accession Version XM_005636319.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2136bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636319.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGCT TCAAACACTC
    CAGAGTGAAA ATTCGAACCT CAGGAAACAG GCCACAACCA ATATATATCA GGTGCAAACT
    GGTTCTGAGT ACACAGACAC TTCCAACCAC TCTTCCTTAA AGCGACGTCC GTCTGCCCGG
    GGCAGTAGGC CCATGTCCAT GTACGAGACG GGATCAGGTC AGAAACCATA TCTCCCAATG
    GGAGAAGTGA ACCACCCTGA AGAGAGCAGG ACAAGACTCC AGCCTTTCCC CACGCACATC
    GGGAGGAGTG CGCTTGTGAC CTCCTCTTCA TCTCTGCCTT CCTTCCCCTC CACACTTTCC
    TGGTCGAGGG ACGAAAGCGC CCGAAGGGCC TCCAGGCTAG AAAAGCAGAA CAGCACGCCT
    GAAAGTGACT ACGATAATAC ACCCAATGAC ACGGACCCAG ATGACACAGG GTCAAGCAGA
    AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA GGCGATGGCT CAGTACCAGA CACAGTGGAA
    CCCCACACTG CCCCCAGCCC CATCCTCCCC AGCACGGAAG ACGTCATCCG GAAAACTGAA
    CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCCGCTC AAGAAAATAA ACATGACAGC
    TATATCCCCT GCTCAGAAAG GATACATGTA GCTGTTACCG AAATGGCAGC ATTATTCCCC
    AAAAAACCCA AGTCTGACAC GGTGAGGACT TCCCTGCGCC TGCTGACATC CAGTGCCTAC
    CGACTCCAGT CAGAGTGCAA GAAGACCCTC CCCGGGGACC CCGGCCCGCC CACGGACATC
    CAGCTGGTCA CACAGCAGGT CATCCAGTGT GCATATGACA TTGCCAAGGC CGCCAAGCAG
    CTGGTCACCA TCACCACCAA AGAGAACAAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636376.1
    CDS136..2271
    Translation

    Target ORF information:

    RefSeq Version XM_005636319.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636319.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGCT TCAAACACTC
    CAGAGTGAAA ATTCGAACCT CAGGAAACAG GCCACAACCA ATATATATCA GGTGCAAACT
    GGTTCTGAGT ACACAGACAC TTCCAACCAC TCTTCCTTAA AGCGACGTCC GTCTGCCCGG
    GGCAGTAGGC CCATGTCCAT GTACGAGACG GGATCAGGTC AGAAACCATA TCTCCCAATG
    GGAGAAGTGA ACCACCCTGA AGAGAGCAGG ACAAGACTCC AGCCTTTCCC CACGCACATC
    GGGAGGAGTG CGCTTGTGAC CTCCTCTTCA TCTCTGCCTT CCTTCCCCTC CACACTTTCC
    TGGTCGAGGG ACGAAAGCGC CCGAAGGGCC TCCAGGCTAG AAAAGCAGAA CAGCACGCCT
    GAAAGTGACT ACGATAATAC ACCCAATGAC ACGGACCCAG ATGACACAGG GTCAAGCAGA
    AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA GGCGATGGCT CAGTACCAGA CACAGTGGAA
    CCCCACACTG CCCCCAGCCC CATCCTCCCC AGCACGGAAG ACGTCATCCG GAAAACTGAA
    CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCCGCTC AAGAAAATAA ACATGACAGC
    TATATCCCCT GCTCAGAAAG GATACATGTA GCTGTTACCG AAATGGCAGC ATTATTCCCC
    AAAAAACCCA AGTCTGACAC GGTGAGGACT TCCCTGCGCC TGCTGACATC CAGTGCCTAC
    CGACTCCAGT CAGAGTGCAA GAAGACCCTC CCCGGGGACC CCGGCCCGCC CACGGACATC
    CAGCTGGTCA CACAGCAGGT CATCCAGTGT GCATATGACA TTGCCAAGGC CGCCAAGCAG
    CTGGTCACCA TCACCACCAA AGAGAACAAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26371
    Clone ID Related Accession (Same CDS sequence) XM_005636319.2 , XM_005636319.3
    Accession Version XM_005636319.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2136bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636319.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGCT TCAAACACTC
    CAGAGTGAAA ATTCGAACCT CAGGAAACAG GCCACAACCA ATATATATCA GGTGCAAACT
    GGTTCTGAGT ACACAGACAC TTCCAACCAC TCTTCCTTAA AGCGACGTCC GTCTGCCCGG
    GGCAGTAGGC CCATGTCCAT GTACGAGACG GGATCAGGTC AGAAACCATA TCTCCCAATG
    GGAGAAGTGA ACCACCCTGA AGAGAGCAGG ACAAGACTCC AGCCTTTCCC CACGCACATC
    GGGAGGAGTG CGCTTGTGAC CTCCTCTTCA TCTCTGCCTT CCTTCCCCTC CACACTTTCC
    TGGTCGAGGG ACGAAAGCGC CCGAAGGGCC TCCAGGCTAG AAAAGCAGAA CAGCACGCCT
    GAAAGTGACT ACGATAATAC ACCCAATGAC ACGGACCCAG ATGACACAGG GTCAAGCAGA
    AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA GGCGATGGCT CAGTACCAGA CACAGTGGAA
    CCCCACACTG CCCCCAGCCC CATCCTCCCC AGCACGGAAG ACGTCATCCG GAAAACTGAA
    CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCCGCTC AAGAAAATAA ACATGACAGC
    TATATCCCCT GCTCAGAAAG GATACATGTA GCTGTTACCG AAATGGCAGC ATTATTCCCC
    AAAAAACCCA AGTCTGACAC GGTGAGGACT TCCCTGCGCC TGCTGACATC CAGTGCCTAC
    CGACTCCAGT CAGAGTGCAA GAAGACCCTC CCCGGGGACC CCGGCCCGCC CACGGACATC
    CAGCTGGTCA CACAGCAGGT CATCCAGTGT GCATATGACA TTGCCAAGGC CGCCAAGCAG
    CTGGTCACCA TCACCACCAA AGAGAACAAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636376.1
    CDS135..2270
    Translation

    Target ORF information:

    RefSeq Version XM_005636319.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636319.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGCT TCAAACACTC
    CAGAGTGAAA ATTCGAACCT CAGGAAACAG GCCACAACCA ATATATATCA GGTGCAAACT
    GGTTCTGAGT ACACAGACAC TTCCAACCAC TCTTCCTTAA AGCGACGTCC GTCTGCCCGG
    GGCAGTAGGC CCATGTCCAT GTACGAGACG GGATCAGGTC AGAAACCATA TCTCCCAATG
    GGAGAAGTGA ACCACCCTGA AGAGAGCAGG ACAAGACTCC AGCCTTTCCC CACGCACATC
    GGGAGGAGTG CGCTTGTGAC CTCCTCTTCA TCTCTGCCTT CCTTCCCCTC CACACTTTCC
    TGGTCGAGGG ACGAAAGCGC CCGAAGGGCC TCCAGGCTAG AAAAGCAGAA CAGCACGCCT
    GAAAGTGACT ACGATAATAC ACCCAATGAC ACGGACCCAG ATGACACAGG GTCAAGCAGA
    AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA GGCGATGGCT CAGTACCAGA CACAGTGGAA
    CCCCACACTG CCCCCAGCCC CATCCTCCCC AGCACGGAAG ACGTCATCCG GAAAACTGAA
    CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCCGCTC AAGAAAATAA ACATGACAGC
    TATATCCCCT GCTCAGAAAG GATACATGTA GCTGTTACCG AAATGGCAGC ATTATTCCCC
    AAAAAACCCA AGTCTGACAC GGTGAGGACT TCCCTGCGCC TGCTGACATC CAGTGCCTAC
    CGACTCCAGT CAGAGTGCAA GAAGACCCTC CCCGGGGACC CCGGCCCGCC CACGGACATC
    CAGCTGGTCA CACAGCAGGT CATCCAGTGT GCATATGACA TTGCCAAGGC CGCCAAGCAG
    CTGGTCACCA TCACCACCAA AGAGAACAAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26372
    Clone ID Related Accession (Same CDS sequence) XM_005636320.3 , XM_005636320.2
    Accession Version XM_005636320.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636320.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTCCAA TCCCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAAAACCACA GCACCCTGGT AACCGAGACA ACTGTCGTCC CCTTCCTTCC GGTCAATCCT
    GAGTACTCTT CAACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACAC TGGTCATCGA CATTCTCAGT GACGCCAAGA GGAGACAGCA AGGCAGTCCT
    CTTTCTGGTT CTAAAGACAA TGTGGAGCTG ATACTGAAAA CGATCAGTAA CCAGCACAGC
    ATTGAGAGTC AAGACAATGA CCAGCCAGAC TATGACAGTG TGGCCTCAGA CGAAGACACG
    GATCTGGAAG CTACTGCAAG CAAAGCAAAC AGGCAGAAGC TTCAAACACT CCAGAGTGAA
    AATTCGAACC TCAGGAAACA GGCCACAACC AATATATATC AGGTGCAAAC TGGTTCTGAG
    TACACAGACA CTTCCAACCA CTCTTCCTTA AAGCGACGTC CGTCTGCCCG GGGCAGTAGG
    CCCATGTCCA TGTACGAGAC GGGATCAGGT CAGAAACCAT ATCTCCCAAT GGGAGAAGTG
    AACCACCCTG AAGAGAGCAG GACAAGACTC CAGCCTTTCC CCACGCACAT CGGGAGGAGT
    GCGCTTGTGA CCTCCTCTTC ATCTCTGCCT TCCTTCCCCT CCACACTTTC CTGGTCGAGG
    GACGAAAGCG CCCGAAGGGC CTCCAGGCTA GAAAAGCAGA ACAGCACGCC TGAAAGTGAC
    TACGATAATA CACCCAATGA CACGGACCCA GATGACACAG GGTCAAGCAG AAAGGGAAGG
    CAGAGGAGTA TGGTGTGGCA AGGCGATGGC TCAGTACCAG ACACAGTGGA ACCCCACACT
    GCCCCCAGCC CCATCCTCCC CAGCACGGAA GACGTCATCC GGAAAACTGA ACAGATCACC
    AAAAACATAC AGGAGCTCTT AAGAGCCGCT CAAGAAAATA AACATGACAG CTATATCCCC
    TGCTCAGAAA GGATACATGT AGCTGTTACC GAAATGGCAG CATTATTCCC CAAAAAACCC
    AAGTCTGACA CGGTGAGGAC TTCCCTGCGC CTGCTGACAT CCAGTGCCTA CCGACTCCAG
    TCAGAGTGCA AGAAGACCCT CCCCGGGGAC CCCGGCCCGC CCACGGACAT CCAGCTGGTC
    ACACAGCAGG TCATCCAGTG TGCATATGAC ATTGCCAAGG CCGCCAAGCA GCTGGTCACC
    ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636377.1
    CDS135..2261
    Translation

    Target ORF information:

    RefSeq Version XM_005636320.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636320.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTCCAA TCCCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAAAACCACA GCACCCTGGT AACCGAGACA ACTGTCGTCC CCTTCCTTCC GGTCAATCCT
    GAGTACTCTT CAACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACAC TGGTCATCGA CATTCTCAGT GACGCCAAGA GGAGACAGCA AGGCAGTCCT
    CTTTCTGGTT CTAAAGACAA TGTGGAGCTG ATACTGAAAA CGATCAGTAA CCAGCACAGC
    ATTGAGAGTC AAGACAATGA CCAGCCAGAC TATGACAGTG TGGCCTCAGA CGAAGACACG
    GATCTGGAAG CTACTGCAAG CAAAGCAAAC AGGCAGAAGC TTCAAACACT CCAGAGTGAA
    AATTCGAACC TCAGGAAACA GGCCACAACC AATATATATC AGGTGCAAAC TGGTTCTGAG
    TACACAGACA CTTCCAACCA CTCTTCCTTA AAGCGACGTC CGTCTGCCCG GGGCAGTAGG
    CCCATGTCCA TGTACGAGAC GGGATCAGGT CAGAAACCAT ATCTCCCAAT GGGAGAAGTG
    AACCACCCTG AAGAGAGCAG GACAAGACTC CAGCCTTTCC CCACGCACAT CGGGAGGAGT
    GCGCTTGTGA CCTCCTCTTC ATCTCTGCCT TCCTTCCCCT CCACACTTTC CTGGTCGAGG
    GACGAAAGCG CCCGAAGGGC CTCCAGGCTA GAAAAGCAGA ACAGCACGCC TGAAAGTGAC
    TACGATAATA CACCCAATGA CACGGACCCA GATGACACAG GGTCAAGCAG AAAGGGAAGG
    CAGAGGAGTA TGGTGTGGCA AGGCGATGGC TCAGTACCAG ACACAGTGGA ACCCCACACT
    GCCCCCAGCC CCATCCTCCC CAGCACGGAA GACGTCATCC GGAAAACTGA ACAGATCACC
    AAAAACATAC AGGAGCTCTT AAGAGCCGCT CAAGAAAATA AACATGACAG CTATATCCCC
    TGCTCAGAAA GGATACATGT AGCTGTTACC GAAATGGCAG CATTATTCCC CAAAAAACCC
    AAGTCTGACA CGGTGAGGAC TTCCCTGCGC CTGCTGACAT CCAGTGCCTA CCGACTCCAG
    TCAGAGTGCA AGAAGACCCT CCCCGGGGAC CCCGGCCCGC CCACGGACAT CCAGCTGGTC
    ACACAGCAGG TCATCCAGTG TGCATATGAC ATTGCCAAGG CCGCCAAGCA GCTGGTCACC
    ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26372
    Clone ID Related Accession (Same CDS sequence) XM_005636320.3 , XM_005636320.2
    Accession Version XM_005636320.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTCCAA TCCCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAAAACCACA GCACCCTGGT AACCGAGACA ACTGTCGTCC CCTTCCTTCC GGTCAATCCT
    GAGTACTCTT CAACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACAC TGGTCATCGA CATTCTCAGT GACGCCAAGA GGAGACAGCA AGGCAGTCCT
    CTTTCTGGTT CTAAAGACAA TGTGGAGCTG ATACTGAAAA CGATCAGTAA CCAGCACAGC
    ATTGAGAGTC AAGACAATGA CCAGCCAGAC TATGACAGTG TGGCCTCAGA CGAAGACACG
    GATCTGGAAG CTACTGCAAG CAAAGCAAAC AGGCAGAAGC TTCAAACACT CCAGAGTGAA
    AATTCGAACC TCAGGAAACA GGCCACAACC AATATATATC AGGTGCAAAC TGGTTCTGAG
    TACACAGACA CTTCCAACCA CTCTTCCTTA AAGCGACGTC CGTCTGCCCG GGGCAGTAGG
    CCCATGTCCA TGTACGAGAC GGGATCAGGT CAGAAACCAT ATCTCCCAAT GGGAGAAGTG
    AACCACCCTG AAGAGAGCAG GACAAGACTC CAGCCTTTCC CCACGCACAT CGGGAGGAGT
    GCGCTTGTGA CCTCCTCTTC ATCTCTGCCT TCCTTCCCCT CCACACTTTC CTGGTCGAGG
    GACGAAAGCG CCCGAAGGGC CTCCAGGCTA GAAAAGCAGA ACAGCACGCC TGAAAGTGAC
    TACGATAATA CACCCAATGA CACGGACCCA GATGACACAG GGTCAAGCAG AAAGGGAAGG
    CAGAGGAGTA TGGTGTGGCA AGGCGATGGC TCAGTACCAG ACACAGTGGA ACCCCACACT
    GCCCCCAGCC CCATCCTCCC CAGCACGGAA GACGTCATCC GGAAAACTGA ACAGATCACC
    AAAAACATAC AGGAGCTCTT AAGAGCCGCT CAAGAAAATA AACATGACAG CTATATCCCC
    TGCTCAGAAA GGATACATGT AGCTGTTACC GAAATGGCAG CATTATTCCC CAAAAAACCC
    AAGTCTGACA CGGTGAGGAC TTCCCTGCGC CTGCTGACAT CCAGTGCCTA CCGACTCCAG
    TCAGAGTGCA AGAAGACCCT CCCCGGGGAC CCCGGCCCGC CCACGGACAT CCAGCTGGTC
    ACACAGCAGG TCATCCAGTG TGCATATGAC ATTGCCAAGG CCGCCAAGCA GCTGGTCACC
    ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636377.1
    CDS136..2262
    Translation

    Target ORF information:

    RefSeq Version XM_005636320.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636320.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTCCAA TCCCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAAAACCACA GCACCCTGGT AACCGAGACA ACTGTCGTCC CCTTCCTTCC GGTCAATCCT
    GAGTACTCTT CAACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACAC TGGTCATCGA CATTCTCAGT GACGCCAAGA GGAGACAGCA AGGCAGTCCT
    CTTTCTGGTT CTAAAGACAA TGTGGAGCTG ATACTGAAAA CGATCAGTAA CCAGCACAGC
    ATTGAGAGTC AAGACAATGA CCAGCCAGAC TATGACAGTG TGGCCTCAGA CGAAGACACG
    GATCTGGAAG CTACTGCAAG CAAAGCAAAC AGGCAGAAGC TTCAAACACT CCAGAGTGAA
    AATTCGAACC TCAGGAAACA GGCCACAACC AATATATATC AGGTGCAAAC TGGTTCTGAG
    TACACAGACA CTTCCAACCA CTCTTCCTTA AAGCGACGTC CGTCTGCCCG GGGCAGTAGG
    CCCATGTCCA TGTACGAGAC GGGATCAGGT CAGAAACCAT ATCTCCCAAT GGGAGAAGTG
    AACCACCCTG AAGAGAGCAG GACAAGACTC CAGCCTTTCC CCACGCACAT CGGGAGGAGT
    GCGCTTGTGA CCTCCTCTTC ATCTCTGCCT TCCTTCCCCT CCACACTTTC CTGGTCGAGG
    GACGAAAGCG CCCGAAGGGC CTCCAGGCTA GAAAAGCAGA ACAGCACGCC TGAAAGTGAC
    TACGATAATA CACCCAATGA CACGGACCCA GATGACACAG GGTCAAGCAG AAAGGGAAGG
    CAGAGGAGTA TGGTGTGGCA AGGCGATGGC TCAGTACCAG ACACAGTGGA ACCCCACACT
    GCCCCCAGCC CCATCCTCCC CAGCACGGAA GACGTCATCC GGAAAACTGA ACAGATCACC
    AAAAACATAC AGGAGCTCTT AAGAGCCGCT CAAGAAAATA AACATGACAG CTATATCCCC
    TGCTCAGAAA GGATACATGT AGCTGTTACC GAAATGGCAG CATTATTCCC CAAAAAACCC
    AAGTCTGACA CGGTGAGGAC TTCCCTGCGC CTGCTGACAT CCAGTGCCTA CCGACTCCAG
    TCAGAGTGCA AGAAGACCCT CCCCGGGGAC CCCGGCCCGC CCACGGACAT CCAGCTGGTC
    ACACAGCAGG TCATCCAGTG TGCATATGAC ATTGCCAAGG CCGCCAAGCA GCTGGTCACC
    ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26373
    Clone ID Related Accession (Same CDS sequence) XM_858808.4 , XM_858808.5
    Accession Version XM_858808.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_858808.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGCT TCAAACACTC
    CAGAGTGAAA ATTCGAACCT CAGGAAACAG GCCACAACCA ATATATATCA GGTGCAAACT
    GGTTCTGAGT ACACAGACAC TTCCAACCAC TCTTCCTTAA AGCGACGTCC GTCTGCCCGG
    GGCAGTAGGC CCATGTCCAT GTACGAGACG GGATCAGGTC AGAAACCATA TCTCCCAATG
    GGAGAAGTGA ACCACCCTGA AGAGAGCAGG ACAAGACTCC AGCCTTTCCC CACGCACGCC
    TCCAGGCTAG AAAAGCAGAA CAGCACGCCT GAAAGTGACT ACGATAATAC ACCCAATGAC
    ACGGACCCAG ATGACACAGG GTCAAGCAGA AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA
    GGCGATGGCT CAGTACCAGA CACAGTGGAA CCCCACACTG CCCCCAGCCC CATCCTCCCC
    AGCACGGAAG ACGTCATCCG GAAAACTGAA CAGATCACCA AAAACATACA GGAGCTCTTA
    AGAGCCGCTC AAGAAAATAA ACATGACAGC TATATCCCCT GCTCAGAAAG GATACATGTA
    GCTGTTACCG AAATGGCAGC ATTATTCCCC AAAAAACCCA AGTCTGACAC GGTGAGGACT
    TCCCTGCGCC TGCTGACATC CAGTGCCTAC CGACTCCAGT CAGAGTGCAA GAAGACCCTC
    CCCGGGGACC CCGGCCCGCC CACGGACATC CAGCTGGTCA CACAGCAGGT CATCCAGTGT
    GCATATGACA TTGCCAAGGC CGCCAAGCAG CTGGTCACCA TCACCACCAA AGAGAACAAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_863901.2
    CDS136..2181
    Translation

    Target ORF information:

    RefSeq Version XM_858808.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_858808.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGCT TCAAACACTC
    CAGAGTGAAA ATTCGAACCT CAGGAAACAG GCCACAACCA ATATATATCA GGTGCAAACT
    GGTTCTGAGT ACACAGACAC TTCCAACCAC TCTTCCTTAA AGCGACGTCC GTCTGCCCGG
    GGCAGTAGGC CCATGTCCAT GTACGAGACG GGATCAGGTC AGAAACCATA TCTCCCAATG
    GGAGAAGTGA ACCACCCTGA AGAGAGCAGG ACAAGACTCC AGCCTTTCCC CACGCACGCC
    TCCAGGCTAG AAAAGCAGAA CAGCACGCCT GAAAGTGACT ACGATAATAC ACCCAATGAC
    ACGGACCCAG ATGACACAGG GTCAAGCAGA AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA
    GGCGATGGCT CAGTACCAGA CACAGTGGAA CCCCACACTG CCCCCAGCCC CATCCTCCCC
    AGCACGGAAG ACGTCATCCG GAAAACTGAA CAGATCACCA AAAACATACA GGAGCTCTTA
    AGAGCCGCTC AAGAAAATAA ACATGACAGC TATATCCCCT GCTCAGAAAG GATACATGTA
    GCTGTTACCG AAATGGCAGC ATTATTCCCC AAAAAACCCA AGTCTGACAC GGTGAGGACT
    TCCCTGCGCC TGCTGACATC CAGTGCCTAC CGACTCCAGT CAGAGTGCAA GAAGACCCTC
    CCCGGGGACC CCGGCCCGCC CACGGACATC CAGCTGGTCA CACAGCAGGT CATCCAGTGT
    GCATATGACA TTGCCAAGGC CGCCAAGCAG CTGGTCACCA TCACCACCAA AGAGAACAAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26373
    Clone ID Related Accession (Same CDS sequence) XM_858808.4 , XM_858808.5
    Accession Version XM_858808.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_858808.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGCT TCAAACACTC
    CAGAGTGAAA ATTCGAACCT CAGGAAACAG GCCACAACCA ATATATATCA GGTGCAAACT
    GGTTCTGAGT ACACAGACAC TTCCAACCAC TCTTCCTTAA AGCGACGTCC GTCTGCCCGG
    GGCAGTAGGC CCATGTCCAT GTACGAGACG GGATCAGGTC AGAAACCATA TCTCCCAATG
    GGAGAAGTGA ACCACCCTGA AGAGAGCAGG ACAAGACTCC AGCCTTTCCC CACGCACGCC
    TCCAGGCTAG AAAAGCAGAA CAGCACGCCT GAAAGTGACT ACGATAATAC ACCCAATGAC
    ACGGACCCAG ATGACACAGG GTCAAGCAGA AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA
    GGCGATGGCT CAGTACCAGA CACAGTGGAA CCCCACACTG CCCCCAGCCC CATCCTCCCC
    AGCACGGAAG ACGTCATCCG GAAAACTGAA CAGATCACCA AAAACATACA GGAGCTCTTA
    AGAGCCGCTC AAGAAAATAA ACATGACAGC TATATCCCCT GCTCAGAAAG GATACATGTA
    GCTGTTACCG AAATGGCAGC ATTATTCCCC AAAAAACCCA AGTCTGACAC GGTGAGGACT
    TCCCTGCGCC TGCTGACATC CAGTGCCTAC CGACTCCAGT CAGAGTGCAA GAAGACCCTC
    CCCGGGGACC CCGGCCCGCC CACGGACATC CAGCTGGTCA CACAGCAGGT CATCCAGTGT
    GCATATGACA TTGCCAAGGC CGCCAAGCAG CTGGTCACCA TCACCACCAA AGAGAACAAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_863901.2
    CDS135..2180
    Translation

    Target ORF information:

    RefSeq Version XM_858808.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_858808.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGCT TCAAACACTC
    CAGAGTGAAA ATTCGAACCT CAGGAAACAG GCCACAACCA ATATATATCA GGTGCAAACT
    GGTTCTGAGT ACACAGACAC TTCCAACCAC TCTTCCTTAA AGCGACGTCC GTCTGCCCGG
    GGCAGTAGGC CCATGTCCAT GTACGAGACG GGATCAGGTC AGAAACCATA TCTCCCAATG
    GGAGAAGTGA ACCACCCTGA AGAGAGCAGG ACAAGACTCC AGCCTTTCCC CACGCACGCC
    TCCAGGCTAG AAAAGCAGAA CAGCACGCCT GAAAGTGACT ACGATAATAC ACCCAATGAC
    ACGGACCCAG ATGACACAGG GTCAAGCAGA AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA
    GGCGATGGCT CAGTACCAGA CACAGTGGAA CCCCACACTG CCCCCAGCCC CATCCTCCCC
    AGCACGGAAG ACGTCATCCG GAAAACTGAA CAGATCACCA AAAACATACA GGAGCTCTTA
    AGAGCCGCTC AAGAAAATAA ACATGACAGC TATATCCCCT GCTCAGAAAG GATACATGTA
    GCTGTTACCG AAATGGCAGC ATTATTCCCC AAAAAACCCA AGTCTGACAC GGTGAGGACT
    TCCCTGCGCC TGCTGACATC CAGTGCCTAC CGACTCCAGT CAGAGTGCAA GAAGACCCTC
    CCCGGGGACC CCGGCCCGCC CACGGACATC CAGCTGGTCA CACAGCAGGT CATCCAGTGT
    GCATATGACA TTGCCAAGGC CGCCAAGCAG CTGGTCACCA TCACCACCAA AGAGAACAAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26374
    Clone ID Related Accession (Same CDS sequence) XM_858892.4 , XM_858892.5
    Accession Version XM_858892.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_858892.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGCTTCAAAC ACTCCAGAGT
    GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATATAT ATCAGGTGCA AACTGGTTCT
    GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT
    AGGCCCATGT CCATGTACGA GACGGGATCA GGTCAGAAAC CATATCTCCC AATGGGAGAA
    GTGAACCACC CTGAAGAGAG CAGGACAAGA CTCCAGCCTT TCCCCACGCA CGCCTCCAGG
    CTAGAAAAGC AGAACAGCAC GCCTGAAAGT GACTACGATA ATACACCCAA TGACACGGAC
    CCAGATGACA CAGGGTCAAG CAGAAAGGGA AGGCAGAGGA GTATGGTGTG GCAAGGCGAT
    GGCTCAGTAC CAGACACAGT GGAACCCCAC ACTGCCCCCA GCCCCATCCT CCCCAGCACG
    GAAGACGTCA TCCGGAAAAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCC
    GCTCAAGAAA ATAAACATGA CAGCTATATC CCCTGCTCAG AAAGGATACA TGTAGCTGTT
    ACCGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ACACGGTGAG GACTTCCCTG
    CGCCTGCTGA CATCCAGTGC CTACCGACTC CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG
    GACCCCGGCC CGCCCACGGA CATCCAGCTG GTCACACAGC AGGTCATCCA GTGTGCATAT
    GACATTGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_863985.1
    CDS136..2175
    Translation

    Target ORF information:

    RefSeq Version XM_858892.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_858892.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGCTTCAAAC ACTCCAGAGT
    GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATATAT ATCAGGTGCA AACTGGTTCT
    GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT
    AGGCCCATGT CCATGTACGA GACGGGATCA GGTCAGAAAC CATATCTCCC AATGGGAGAA
    GTGAACCACC CTGAAGAGAG CAGGACAAGA CTCCAGCCTT TCCCCACGCA CGCCTCCAGG
    CTAGAAAAGC AGAACAGCAC GCCTGAAAGT GACTACGATA ATACACCCAA TGACACGGAC
    CCAGATGACA CAGGGTCAAG CAGAAAGGGA AGGCAGAGGA GTATGGTGTG GCAAGGCGAT
    GGCTCAGTAC CAGACACAGT GGAACCCCAC ACTGCCCCCA GCCCCATCCT CCCCAGCACG
    GAAGACGTCA TCCGGAAAAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCC
    GCTCAAGAAA ATAAACATGA CAGCTATATC CCCTGCTCAG AAAGGATACA TGTAGCTGTT
    ACCGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ACACGGTGAG GACTTCCCTG
    CGCCTGCTGA CATCCAGTGC CTACCGACTC CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG
    GACCCCGGCC CGCCCACGGA CATCCAGCTG GTCACACAGC AGGTCATCCA GTGTGCATAT
    GACATTGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26374
    Clone ID Related Accession (Same CDS sequence) XM_858892.4 , XM_858892.5
    Accession Version XM_858892.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_858892.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGCTTCAAAC ACTCCAGAGT
    GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATATAT ATCAGGTGCA AACTGGTTCT
    GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT
    AGGCCCATGT CCATGTACGA GACGGGATCA GGTCAGAAAC CATATCTCCC AATGGGAGAA
    GTGAACCACC CTGAAGAGAG CAGGACAAGA CTCCAGCCTT TCCCCACGCA CGCCTCCAGG
    CTAGAAAAGC AGAACAGCAC GCCTGAAAGT GACTACGATA ATACACCCAA TGACACGGAC
    CCAGATGACA CAGGGTCAAG CAGAAAGGGA AGGCAGAGGA GTATGGTGTG GCAAGGCGAT
    GGCTCAGTAC CAGACACAGT GGAACCCCAC ACTGCCCCCA GCCCCATCCT CCCCAGCACG
    GAAGACGTCA TCCGGAAAAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCC
    GCTCAAGAAA ATAAACATGA CAGCTATATC CCCTGCTCAG AAAGGATACA TGTAGCTGTT
    ACCGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ACACGGTGAG GACTTCCCTG
    CGCCTGCTGA CATCCAGTGC CTACCGACTC CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG
    GACCCCGGCC CGCCCACGGA CATCCAGCTG GTCACACAGC AGGTCATCCA GTGTGCATAT
    GACATTGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_863985.1
    CDS135..2174
    Translation

    Target ORF information:

    RefSeq Version XM_858892.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_858892.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGCTTCAAAC ACTCCAGAGT
    GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATATAT ATCAGGTGCA AACTGGTTCT
    GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT
    AGGCCCATGT CCATGTACGA GACGGGATCA GGTCAGAAAC CATATCTCCC AATGGGAGAA
    GTGAACCACC CTGAAGAGAG CAGGACAAGA CTCCAGCCTT TCCCCACGCA CGCCTCCAGG
    CTAGAAAAGC AGAACAGCAC GCCTGAAAGT GACTACGATA ATACACCCAA TGACACGGAC
    CCAGATGACA CAGGGTCAAG CAGAAAGGGA AGGCAGAGGA GTATGGTGTG GCAAGGCGAT
    GGCTCAGTAC CAGACACAGT GGAACCCCAC ACTGCCCCCA GCCCCATCCT CCCCAGCACG
    GAAGACGTCA TCCGGAAAAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCC
    GCTCAAGAAA ATAAACATGA CAGCTATATC CCCTGCTCAG AAAGGATACA TGTAGCTGTT
    ACCGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ACACGGTGAG GACTTCCCTG
    CGCCTGCTGA CATCCAGTGC CTACCGACTC CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG
    GACCCCGGCC CGCCCACGGA CATCCAGCTG GTCACACAGC AGGTCATCCA GTGTGCATAT
    GACATTGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26375
    Clone ID Related Accession (Same CDS sequence) XM_022410811.1
    Accession Version XM_022410811.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGCC TCCAGGCTAG AAAAGCAGAA CAGCACGCCT
    GAAAGTGACT ACGATAATAC ACCCAATGAC ACGGACCCAG ATGACACAGG GTCAAGCAGA
    AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA GGCGATGGCT CAGTACCAGA CACAGTGGAA
    CCCCACACTG CCCCCAGCCC CATCCTCCCC AGCACGGAAG ACGTCATCCG GAAAACTGAA
    CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCCGCTC AAGAAAATAA ACATGACAGC
    TATATCCCCT GCTCAGAAAG GATACATGTA GCTGTTACCG AAATGGCAGC ATTATTCCCC
    AAAAAACCCA AGTCTGACAC GGTGAGGACT TCCCTGCGCC TGCTGACATC CAGTGCCTAC
    CGACTCCAGT CAGAGTGCAA GAAGACCCTC CCCGGGGACC CCGGCCCGCC CACGGACATC
    CAGCTGGTCA CACAGCAGGT CATCCAGTGT GCATATGACA TTGCCAAGGC CGCCAAGCAG
    CTGGTCACCA TCACCACCAA AGAGAACAAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266519.1
    CDS135..2030
    Translation

    Target ORF information:

    RefSeq Version XM_022410811.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410811.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGCC TCCAGGCTAG AAAAGCAGAA CAGCACGCCT
    GAAAGTGACT ACGATAATAC ACCCAATGAC ACGGACCCAG ATGACACAGG GTCAAGCAGA
    AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA GGCGATGGCT CAGTACCAGA CACAGTGGAA
    CCCCACACTG CCCCCAGCCC CATCCTCCCC AGCACGGAAG ACGTCATCCG GAAAACTGAA
    CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCCGCTC AAGAAAATAA ACATGACAGC
    TATATCCCCT GCTCAGAAAG GATACATGTA GCTGTTACCG AAATGGCAGC ATTATTCCCC
    AAAAAACCCA AGTCTGACAC GGTGAGGACT TCCCTGCGCC TGCTGACATC CAGTGCCTAC
    CGACTCCAGT CAGAGTGCAA GAAGACCCTC CCCGGGGACC CCGGCCCGCC CACGGACATC
    CAGCTGGTCA CACAGCAGGT CATCCAGTGT GCATATGACA TTGCCAAGGC CGCCAAGCAG
    CTGGTCACCA TCACCACCAA AGAGAACAAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282644
    Clone ID Related Accession (Same CDS sequence) XM_022410810.1
    Accession Version XM_022410810.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGCCTCCA GGCTAGAAAA GCAGAACAGC
    ACGCCTGAAA GTGACTACGA TAATACACCC AATGACACGG ACCCAGATGA CACAGGGTCA
    AGCAGAAAGG GAAGGCAGAG GAGTATGGTG TGGCAAGGCG ATGGCTCAGT ACCAGACACA
    GTGGAACCCC ACACTGCCCC CAGCCCCATC CTCCCCAGCA CGGAAGACGT CATCCGGAAA
    ACTGAACAGA TCACCAAAAA CATACAGGAG CTCTTAAGAG CCGCTCAAGA AAATAAACAT
    GACAGCTATA TCCCCTGCTC AGAAAGGATA CATGTAGCTG TTACCGAAAT GGCAGCATTA
    TTCCCCAAAA AACCCAAGTC TGACACGGTG AGGACTTCCC TGCGCCTGCT GACATCCAGT
    GCCTACCGAC TCCAGTCAGA GTGCAAGAAG ACCCTCCCCG GGGACCCCGG CCCGCCCACG
    GACATCCAGC TGGTCACACA GCAGGTCATC CAGTGTGCAT ATGACATTGC CAAGGCCGCC
    AAGCAGCTGG TCACCATCAC CACCAAAGAG AACAACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266518.1
    CDS135..2036
    Translation

    Target ORF information:

    RefSeq Version XM_022410810.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410810.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGCCTCCA GGCTAGAAAA GCAGAACAGC
    ACGCCTGAAA GTGACTACGA TAATACACCC AATGACACGG ACCCAGATGA CACAGGGTCA
    AGCAGAAAGG GAAGGCAGAG GAGTATGGTG TGGCAAGGCG ATGGCTCAGT ACCAGACACA
    GTGGAACCCC ACACTGCCCC CAGCCCCATC CTCCCCAGCA CGGAAGACGT CATCCGGAAA
    ACTGAACAGA TCACCAAAAA CATACAGGAG CTCTTAAGAG CCGCTCAAGA AAATAAACAT
    GACAGCTATA TCCCCTGCTC AGAAAGGATA CATGTAGCTG TTACCGAAAT GGCAGCATTA
    TTCCCCAAAA AACCCAAGTC TGACACGGTG AGGACTTCCC TGCGCCTGCT GACATCCAGT
    GCCTACCGAC TCCAGTCAGA GTGCAAGAAG ACCCTCCCCG GGGACCCCGG CCCGCCCACG
    GACATCCAGC TGGTCACACA GCAGGTCATC CAGTGTGCAT ATGACATTGC CAAGGCCGCC
    AAGCAGCTGG TCACCATCAC CACCAAAGAG AACAACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43303
    Clone ID Related Accession (Same CDS sequence) XM_014107944.1 , XM_014107944.2
    Accession Version XM_014107944.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGCT TCAAACACTC CAGAGTGAAA ATTCGAACCT CAGGAAACAG
    GCCACAACCA ATATATATCA GGTGCAAACT GGTTCTGAGT ACACAGACAC TTCCAACCAC
    TCTTCCTTAA AGCGACGTCC GTCTGCCCGG GGCAGTAGGC CCATGTCCAT GTACGAGACG
    GGATCAGGTC AGAAACCATA TCTCCCAATG GGAGAAGTGA ACCACCCTGA AGAGAGCAGG
    ACAAGACTCC AGCCTTTCCC CACGCACGCC TCCAGGCTAG AAAAGCAGAA CAGCACGCCT
    GAAAGTGACT ACGATAATAC ACCCAATGAC ACGGACCCAG ATGACACAGG GTCAAGCAGA
    AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA GGCGATGGCT CAGTACCAGA CACAGTGGAA
    CCCCACACTG CCCCCAGCCC CATCCTCCCC AGCACGGAAG ACGTCATCCG GAAAACTGAA
    CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCCGCTC AAGAAAATAA ACATGACAGC
    TATATCCCCT GCTCAGAAAG GATACATGTA GCTGTTACCG AAATGGCAGC ATTATTCCCC
    AAAAAACCCA AGTCTGACAC GGTGAGGACT TCCCTGCGCC TGCTGACATC CAGTGCCTAC
    CGACTCCAGT CAGAGTGCAA GAAGACCCTC CCCGGGGACC CCGGCCCGCC CACGGACATC
    CAGCTGGTCA CACAGCAGGT CATCCAGTGT GCATATGACA TTGCCAAGGC CGCCAAGCAG
    CTGGTCACCA TCACCACCAA AGAGAACAAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963419.1
    CDS136..2331
    Translation

    Target ORF information:

    RefSeq Version XM_014107944.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107944.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGCT TCAAACACTC CAGAGTGAAA ATTCGAACCT CAGGAAACAG
    GCCACAACCA ATATATATCA GGTGCAAACT GGTTCTGAGT ACACAGACAC TTCCAACCAC
    TCTTCCTTAA AGCGACGTCC GTCTGCCCGG GGCAGTAGGC CCATGTCCAT GTACGAGACG
    GGATCAGGTC AGAAACCATA TCTCCCAATG GGAGAAGTGA ACCACCCTGA AGAGAGCAGG
    ACAAGACTCC AGCCTTTCCC CACGCACGCC TCCAGGCTAG AAAAGCAGAA CAGCACGCCT
    GAAAGTGACT ACGATAATAC ACCCAATGAC ACGGACCCAG ATGACACAGG GTCAAGCAGA
    AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA GGCGATGGCT CAGTACCAGA CACAGTGGAA
    CCCCACACTG CCCCCAGCCC CATCCTCCCC AGCACGGAAG ACGTCATCCG GAAAACTGAA
    CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCCGCTC AAGAAAATAA ACATGACAGC
    TATATCCCCT GCTCAGAAAG GATACATGTA GCTGTTACCG AAATGGCAGC ATTATTCCCC
    AAAAAACCCA AGTCTGACAC GGTGAGGACT TCCCTGCGCC TGCTGACATC CAGTGCCTAC
    CGACTCCAGT CAGAGTGCAA GAAGACCCTC CCCGGGGACC CCGGCCCGCC CACGGACATC
    CAGCTGGTCA CACAGCAGGT CATCCAGTGT GCATATGACA TTGCCAAGGC CGCCAAGCAG
    CTGGTCACCA TCACCACCAA AGAGAACAAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43303
    Clone ID Related Accession (Same CDS sequence) XM_014107944.1 , XM_014107944.2
    Accession Version XM_014107944.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107944.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGCT TCAAACACTC CAGAGTGAAA ATTCGAACCT CAGGAAACAG
    GCCACAACCA ATATATATCA GGTGCAAACT GGTTCTGAGT ACACAGACAC TTCCAACCAC
    TCTTCCTTAA AGCGACGTCC GTCTGCCCGG GGCAGTAGGC CCATGTCCAT GTACGAGACG
    GGATCAGGTC AGAAACCATA TCTCCCAATG GGAGAAGTGA ACCACCCTGA AGAGAGCAGG
    ACAAGACTCC AGCCTTTCCC CACGCACGCC TCCAGGCTAG AAAAGCAGAA CAGCACGCCT
    GAAAGTGACT ACGATAATAC ACCCAATGAC ACGGACCCAG ATGACACAGG GTCAAGCAGA
    AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA GGCGATGGCT CAGTACCAGA CACAGTGGAA
    CCCCACACTG CCCCCAGCCC CATCCTCCCC AGCACGGAAG ACGTCATCCG GAAAACTGAA
    CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCCGCTC AAGAAAATAA ACATGACAGC
    TATATCCCCT GCTCAGAAAG GATACATGTA GCTGTTACCG AAATGGCAGC ATTATTCCCC
    AAAAAACCCA AGTCTGACAC GGTGAGGACT TCCCTGCGCC TGCTGACATC CAGTGCCTAC
    CGACTCCAGT CAGAGTGCAA GAAGACCCTC CCCGGGGACC CCGGCCCGCC CACGGACATC
    CAGCTGGTCA CACAGCAGGT CATCCAGTGT GCATATGACA TTGCCAAGGC CGCCAAGCAG
    CTGGTCACCA TCACCACCAA AGAGAACAAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963419.1
    CDS135..2330
    Translation

    Target ORF information:

    RefSeq Version XM_014107944.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107944.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGCT TCAAACACTC CAGAGTGAAA ATTCGAACCT CAGGAAACAG
    GCCACAACCA ATATATATCA GGTGCAAACT GGTTCTGAGT ACACAGACAC TTCCAACCAC
    TCTTCCTTAA AGCGACGTCC GTCTGCCCGG GGCAGTAGGC CCATGTCCAT GTACGAGACG
    GGATCAGGTC AGAAACCATA TCTCCCAATG GGAGAAGTGA ACCACCCTGA AGAGAGCAGG
    ACAAGACTCC AGCCTTTCCC CACGCACGCC TCCAGGCTAG AAAAGCAGAA CAGCACGCCT
    GAAAGTGACT ACGATAATAC ACCCAATGAC ACGGACCCAG ATGACACAGG GTCAAGCAGA
    AAGGGAAGGC AGAGGAGTAT GGTGTGGCAA GGCGATGGCT CAGTACCAGA CACAGTGGAA
    CCCCACACTG CCCCCAGCCC CATCCTCCCC AGCACGGAAG ACGTCATCCG GAAAACTGAA
    CAGATCACCA AAAACATACA GGAGCTCTTA AGAGCCGCTC AAGAAAATAA ACATGACAGC
    TATATCCCCT GCTCAGAAAG GATACATGTA GCTGTTACCG AAATGGCAGC ATTATTCCCC
    AAAAAACCCA AGTCTGACAC GGTGAGGACT TCCCTGCGCC TGCTGACATC CAGTGCCTAC
    CGACTCCAGT CAGAGTGCAA GAAGACCCTC CCCGGGGACC CCGGCCCGCC CACGGACATC
    CAGCTGGTCA CACAGCAGGT CATCCAGTGT GCATATGACA TTGCCAAGGC CGCCAAGCAG
    CTGGTCACCA TCACCACCAA AGAGAACAAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43304
    Clone ID Related Accession (Same CDS sequence) XM_014107945.2 , XM_014107945.1
    Accession Version XM_014107945.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2100bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGTTGAAA CTTTGTATAA TAATGGTGCT AATTCTATAT GGGAACATTC TTTGCTGGAC 
    CCTGCCTCTA TTATGAGCGG AAGACGTAAA GCTAATCCAC AGGATAAAGT ACATCCTAAT
    AAAGCAGAAT TCATCAGAGC CAAATATCAG ATGTTAGCAT TTGTCCATCG CTTGCCTTGC
    CGGGATGATG ACAGTATGAC TGCCAAAGAT CTTAGCAAGC AACTTCATTC AAGCGTGAGA
    ACAGGGAATC TTGAAACCTG TTTGAGGCTG TTATCTTTAG GAGCACAAGC CAACTTCTTT
    CACCCTGAAA AAGGAAACAC CCCACTCCAC GTCGCCTCCA AAGCAGGGCA GATTTTACAG
    GCAGAATTAT TGGCAGTATA TGGAGCAGAC CCAGGCACAC AGGATTCTAG TGGAAAAACT
    CCTGTGGATT ATGCAAGGCA AGGAGGGCAC CATGAACTGG CGGAACGCCT TGTCGAAATA
    CAGTATGAGC TGACTGACAG ACTAGCCTTC TATCTCTGTG GCAGGAAACC AGATCACAAA
    AATGGACAGC ACTTTATAAT ACCTCAAATG GCAGACAGAC GGCTCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACGGGATCA
    GGTCAGAAAC CATATCTCCC AATGGGAGAA GTGAACCACC CTGAAGAGAG CAGGACAAGA
    CTCCAGCCTT TCCCCACGCA CATCGGGAGG AGTGCGCTTG TGACCTCCTC TTCATCTCTG
    CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCCTCCAGG
    CTAGAAAAGC AGAACAGCAC GCCTGAAAGT GACTACGATA ATACACCCAA TGACACGGAC
    CCAGATGACA CAGGGTCAAG CAGAAAGGGA AGGCAGAGGA GTATGGTGTG GCAAGGCGAT
    GGCTCAGTAC CAGACACAGT GGAACCCCAC ACTGCCCCCA GCCCCATCCT CCCCAGCACG
    GAAGACGTCA TCCGGAAAAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCC
    GCTCAAGAAA ATAAACATGA CAGCTATATC CCCTGCTCAG AAAGGATACA TGTAGCTGTT
    ACCGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ACACGGTGAG GACTTCCCTG
    CGCCTGCTGA CATCCAGTGC CTACCGACTC CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG
    GACCCCGGCC CGCCCACGGA CATCCAGCTG GTCACACAGC AGGTCATCCA GTGTGCATAT
    GACATTGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963420.1
    CDS264..2363
    Translation

    Target ORF information:

    RefSeq Version XM_014107945.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107945.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGTTGAAA CTTTGTATAA TAATGGTGCT AATTCTATAT GGGAACATTC TTTGCTGGAC 
    CCTGCCTCTA TTATGAGCGG AAGACGTAAA GCTAATCCAC AGGATAAAGT ACATCCTAAT
    AAAGCAGAAT TCATCAGAGC CAAATATCAG ATGTTAGCAT TTGTCCATCG CTTGCCTTGC
    CGGGATGATG ACAGTATGAC TGCCAAAGAT CTTAGCAAGC AACTTCATTC AAGCGTGAGA
    ACAGGGAATC TTGAAACCTG TTTGAGGCTG TTATCTTTAG GAGCACAAGC CAACTTCTTT
    CACCCTGAAA AAGGAAACAC CCCACTCCAC GTCGCCTCCA AAGCAGGGCA GATTTTACAG
    GCAGAATTAT TGGCAGTATA TGGAGCAGAC CCAGGCACAC AGGATTCTAG TGGAAAAACT
    CCTGTGGATT ATGCAAGGCA AGGAGGGCAC CATGAACTGG CGGAACGCCT TGTCGAAATA
    CAGTATGAGC TGACTGACAG ACTAGCCTTC TATCTCTGTG GCAGGAAACC AGATCACAAA
    AATGGACAGC ACTTTATAAT ACCTCAAATG GCAGACAGAC GGCTCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACGGGATCA
    GGTCAGAAAC CATATCTCCC AATGGGAGAA GTGAACCACC CTGAAGAGAG CAGGACAAGA
    CTCCAGCCTT TCCCCACGCA CATCGGGAGG AGTGCGCTTG TGACCTCCTC TTCATCTCTG
    CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCCTCCAGG
    CTAGAAAAGC AGAACAGCAC GCCTGAAAGT GACTACGATA ATACACCCAA TGACACGGAC
    CCAGATGACA CAGGGTCAAG CAGAAAGGGA AGGCAGAGGA GTATGGTGTG GCAAGGCGAT
    GGCTCAGTAC CAGACACAGT GGAACCCCAC ACTGCCCCCA GCCCCATCCT CCCCAGCACG
    GAAGACGTCA TCCGGAAAAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCC
    GCTCAAGAAA ATAAACATGA CAGCTATATC CCCTGCTCAG AAAGGATACA TGTAGCTGTT
    ACCGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ACACGGTGAG GACTTCCCTG
    CGCCTGCTGA CATCCAGTGC CTACCGACTC CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG
    GACCCCGGCC CGCCCACGGA CATCCAGCTG GTCACACAGC AGGTCATCCA GTGTGCATAT
    GACATTGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43304
    Clone ID Related Accession (Same CDS sequence) XM_014107945.2 , XM_014107945.1
    Accession Version XM_014107945.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2100bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGTTGAAA CTTTGTATAA TAATGGTGCT AATTCTATAT GGGAACATTC TTTGCTGGAC 
    CCTGCCTCTA TTATGAGCGG AAGACGTAAA GCTAATCCAC AGGATAAAGT ACATCCTAAT
    AAAGCAGAAT TCATCAGAGC CAAATATCAG ATGTTAGCAT TTGTCCATCG CTTGCCTTGC
    CGGGATGATG ACAGTATGAC TGCCAAAGAT CTTAGCAAGC AACTTCATTC AAGCGTGAGA
    ACAGGGAATC TTGAAACCTG TTTGAGGCTG TTATCTTTAG GAGCACAAGC CAACTTCTTT
    CACCCTGAAA AAGGAAACAC CCCACTCCAC GTCGCCTCCA AAGCAGGGCA GATTTTACAG
    GCAGAATTAT TGGCAGTATA TGGAGCAGAC CCAGGCACAC AGGATTCTAG TGGAAAAACT
    CCTGTGGATT ATGCAAGGCA AGGAGGGCAC CATGAACTGG CGGAACGCCT TGTCGAAATA
    CAGTATGAGC TGACTGACAG ACTAGCCTTC TATCTCTGTG GCAGGAAACC AGATCACAAA
    AATGGACAGC ACTTTATAAT ACCTCAAATG GCAGACAGAC GGCTCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACGGGATCA
    GGTCAGAAAC CATATCTCCC AATGGGAGAA GTGAACCACC CTGAAGAGAG CAGGACAAGA
    CTCCAGCCTT TCCCCACGCA CATCGGGAGG AGTGCGCTTG TGACCTCCTC TTCATCTCTG
    CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCCTCCAGG
    CTAGAAAAGC AGAACAGCAC GCCTGAAAGT GACTACGATA ATACACCCAA TGACACGGAC
    CCAGATGACA CAGGGTCAAG CAGAAAGGGA AGGCAGAGGA GTATGGTGTG GCAAGGCGAT
    GGCTCAGTAC CAGACACAGT GGAACCCCAC ACTGCCCCCA GCCCCATCCT CCCCAGCACG
    GAAGACGTCA TCCGGAAAAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCC
    GCTCAAGAAA ATAAACATGA CAGCTATATC CCCTGCTCAG AAAGGATACA TGTAGCTGTT
    ACCGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ACACGGTGAG GACTTCCCTG
    CGCCTGCTGA CATCCAGTGC CTACCGACTC CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG
    GACCCCGGCC CGCCCACGGA CATCCAGCTG GTCACACAGC AGGTCATCCA GTGTGCATAT
    GACATTGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963420.1
    CDS142..2241
    Translation

    Target ORF information:

    RefSeq Version XM_014107945.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107945.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGTTGAAA CTTTGTATAA TAATGGTGCT AATTCTATAT GGGAACATTC TTTGCTGGAC 
    CCTGCCTCTA TTATGAGCGG AAGACGTAAA GCTAATCCAC AGGATAAAGT ACATCCTAAT
    AAAGCAGAAT TCATCAGAGC CAAATATCAG ATGTTAGCAT TTGTCCATCG CTTGCCTTGC
    CGGGATGATG ACAGTATGAC TGCCAAAGAT CTTAGCAAGC AACTTCATTC AAGCGTGAGA
    ACAGGGAATC TTGAAACCTG TTTGAGGCTG TTATCTTTAG GAGCACAAGC CAACTTCTTT
    CACCCTGAAA AAGGAAACAC CCCACTCCAC GTCGCCTCCA AAGCAGGGCA GATTTTACAG
    GCAGAATTAT TGGCAGTATA TGGAGCAGAC CCAGGCACAC AGGATTCTAG TGGAAAAACT
    CCTGTGGATT ATGCAAGGCA AGGAGGGCAC CATGAACTGG CGGAACGCCT TGTCGAAATA
    CAGTATGAGC TGACTGACAG ACTAGCCTTC TATCTCTGTG GCAGGAAACC AGATCACAAA
    AATGGACAGC ACTTTATAAT ACCTCAAATG GCAGACAGAC GGCTCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTC CAATCCCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAAAACC ACAGCACCCT GGTAACCGAG ACAACTGTCG TCCCCTTCCT TCCGGTCAAT
    CCTGAGTACT CTTCAACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CACTGGTCAT CGACATTCTC AGTGACGCCA AGAGGAGACA GCAAGGCAGT
    CCTCTTTCTG GTTCTAAAGA CAATGTGGAG CTGATACTGA AAACGATCAG TAACCAGCAC
    AGCATTGAGA GTCAAGACAA TGACCAGCCA GACTATGACA GTGTGGCCTC AGACGAAGAC
    ACGGATCTGG AAGCTACTGC AAGCAAAGCA AACAGGCAGA AGAGTCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT ATGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAGCAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACGGGATCA
    GGTCAGAAAC CATATCTCCC AATGGGAGAA GTGAACCACC CTGAAGAGAG CAGGACAAGA
    CTCCAGCCTT TCCCCACGCA CATCGGGAGG AGTGCGCTTG TGACCTCCTC TTCATCTCTG
    CCTTCCTTCC CCTCCACACT TTCCTGGTCG AGGGACGAAA GCGCCCGAAG GGCCTCCAGG
    CTAGAAAAGC AGAACAGCAC GCCTGAAAGT GACTACGATA ATACACCCAA TGACACGGAC
    CCAGATGACA CAGGGTCAAG CAGAAAGGGA AGGCAGAGGA GTATGGTGTG GCAAGGCGAT
    GGCTCAGTAC CAGACACAGT GGAACCCCAC ACTGCCCCCA GCCCCATCCT CCCCAGCACG
    GAAGACGTCA TCCGGAAAAC TGAACAGATC ACCAAAAACA TACAGGAGCT CTTAAGAGCC
    GCTCAAGAAA ATAAACATGA CAGCTATATC CCCTGCTCAG AAAGGATACA TGTAGCTGTT
    ACCGAAATGG CAGCATTATT CCCCAAAAAA CCCAAGTCTG ACACGGTGAG GACTTCCCTG
    CGCCTGCTGA CATCCAGTGC CTACCGACTC CAGTCAGAGT GCAAGAAGAC CCTCCCCGGG
    GACCCCGGCC CGCCCACGGA CATCCAGCTG GTCACACAGC AGGTCATCCA GTGTGCATAT
    GACATTGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43305
    Clone ID Related Accession (Same CDS sequence) XM_014107946.2 , XM_014107946.1
    Accession Version XM_014107946.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107946.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGAT CGGGAGGAGT GCGCTTGTGA CCTCCTCTTC ATCTCTGCCT
    TCCTTCCCCT CCACACTTTC CTGGTCGAGG GACGAAAGCG CCCGAAGGGC CTCCAGGCTA
    GAAAAGCAGA ACAGCACGCC TGAAAGTGAC TACGATAATA CACCCAATGA CACGGACCCA
    GATGACACAG GGTCAAGCAG AAAGGGAAGG CAGAGGAGTA TGGTGTGGCA AGGCGATGGC
    TCAGTACCAG ACACAGTGGA ACCCCACACT GCCCCCAGCC CCATCCTCCC CAGCACGGAA
    GACGTCATCC GGAAAACTGA ACAGATCACC AAAAACATAC AGGAGCTCTT AAGAGCCGCT
    CAAGAAAATA AACATGACAG CTATATCCCC TGCTCAGAAA GGATACATGT AGCTGTTACC
    GAAATGGCAG CATTATTCCC CAAAAAACCC AAGTCTGACA CGGTGAGGAC TTCCCTGCGC
    CTGCTGACAT CCAGTGCCTA CCGACTCCAG TCAGAGTGCA AGAAGACCCT CCCCGGGGAC
    CCCGGCCCGC CCACGGACAT CCAGCTGGTC ACACAGCAGG TCATCCAGTG TGCATATGAC
    ATTGCCAAGG CCGCCAAGCA GCTGGTCACC ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963421.1
    CDS135..2171
    Translation

    Target ORF information:

    RefSeq Version XM_014107946.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107946.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGAT CGGGAGGAGT GCGCTTGTGA CCTCCTCTTC ATCTCTGCCT
    TCCTTCCCCT CCACACTTTC CTGGTCGAGG GACGAAAGCG CCCGAAGGGC CTCCAGGCTA
    GAAAAGCAGA ACAGCACGCC TGAAAGTGAC TACGATAATA CACCCAATGA CACGGACCCA
    GATGACACAG GGTCAAGCAG AAAGGGAAGG CAGAGGAGTA TGGTGTGGCA AGGCGATGGC
    TCAGTACCAG ACACAGTGGA ACCCCACACT GCCCCCAGCC CCATCCTCCC CAGCACGGAA
    GACGTCATCC GGAAAACTGA ACAGATCACC AAAAACATAC AGGAGCTCTT AAGAGCCGCT
    CAAGAAAATA AACATGACAG CTATATCCCC TGCTCAGAAA GGATACATGT AGCTGTTACC
    GAAATGGCAG CATTATTCCC CAAAAAACCC AAGTCTGACA CGGTGAGGAC TTCCCTGCGC
    CTGCTGACAT CCAGTGCCTA CCGACTCCAG TCAGAGTGCA AGAAGACCCT CCCCGGGGAC
    CCCGGCCCGC CCACGGACAT CCAGCTGGTC ACACAGCAGG TCATCCAGTG TGCATATGAC
    ATTGCCAAGG CCGCCAAGCA GCTGGTCACC ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43305
    Clone ID Related Accession (Same CDS sequence) XM_014107946.2 , XM_014107946.1
    Accession Version XM_014107946.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGAT CGGGAGGAGT GCGCTTGTGA CCTCCTCTTC ATCTCTGCCT
    TCCTTCCCCT CCACACTTTC CTGGTCGAGG GACGAAAGCG CCCGAAGGGC CTCCAGGCTA
    GAAAAGCAGA ACAGCACGCC TGAAAGTGAC TACGATAATA CACCCAATGA CACGGACCCA
    GATGACACAG GGTCAAGCAG AAAGGGAAGG CAGAGGAGTA TGGTGTGGCA AGGCGATGGC
    TCAGTACCAG ACACAGTGGA ACCCCACACT GCCCCCAGCC CCATCCTCCC CAGCACGGAA
    GACGTCATCC GGAAAACTGA ACAGATCACC AAAAACATAC AGGAGCTCTT AAGAGCCGCT
    CAAGAAAATA AACATGACAG CTATATCCCC TGCTCAGAAA GGATACATGT AGCTGTTACC
    GAAATGGCAG CATTATTCCC CAAAAAACCC AAGTCTGACA CGGTGAGGAC TTCCCTGCGC
    CTGCTGACAT CCAGTGCCTA CCGACTCCAG TCAGAGTGCA AGAAGACCCT CCCCGGGGAC
    CCCGGCCCGC CCACGGACAT CCAGCTGGTC ACACAGCAGG TCATCCAGTG TGCATATGAC
    ATTGCCAAGG CCGCCAAGCA GCTGGTCACC ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963421.1
    CDS136..2172
    Translation

    Target ORF information:

    RefSeq Version XM_014107946.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107946.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGAT CGGGAGGAGT GCGCTTGTGA CCTCCTCTTC ATCTCTGCCT
    TCCTTCCCCT CCACACTTTC CTGGTCGAGG GACGAAAGCG CCCGAAGGGC CTCCAGGCTA
    GAAAAGCAGA ACAGCACGCC TGAAAGTGAC TACGATAATA CACCCAATGA CACGGACCCA
    GATGACACAG GGTCAAGCAG AAAGGGAAGG CAGAGGAGTA TGGTGTGGCA AGGCGATGGC
    TCAGTACCAG ACACAGTGGA ACCCCACACT GCCCCCAGCC CCATCCTCCC CAGCACGGAA
    GACGTCATCC GGAAAACTGA ACAGATCACC AAAAACATAC AGGAGCTCTT AAGAGCCGCT
    CAAGAAAATA AACATGACAG CTATATCCCC TGCTCAGAAA GGATACATGT AGCTGTTACC
    GAAATGGCAG CATTATTCCC CAAAAAACCC AAGTCTGACA CGGTGAGGAC TTCCCTGCGC
    CTGCTGACAT CCAGTGCCTA CCGACTCCAG TCAGAGTGCA AGAAGACCCT CCCCGGGGAC
    CCCGGCCCGC CCACGGACAT CCAGCTGGTC ACACAGCAGG TCATCCAGTG TGCATATGAC
    ATTGCCAAGG CCGCCAAGCA GCTGGTCACC ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43306
    Clone ID Related Accession (Same CDS sequence) XM_014107947.1 , XM_014107947.2
    Accession Version XM_014107947.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGGC CTCCAGGCTA GAAAAGCAGA ACAGCACGCC TGAAAGTGAC
    TACGATAATA CACCCAATGA CACGGACCCA GATGACACAG GGTCAAGCAG AAAGGGAAGG
    CAGAGGAGTA TGGTGTGGCA AGGCGATGGC TCAGTACCAG ACACAGTGGA ACCCCACACT
    GCCCCCAGCC CCATCCTCCC CAGCACGGAA GACGTCATCC GGAAAACTGA ACAGATCACC
    AAAAACATAC AGGAGCTCTT AAGAGCCGCT CAAGAAAATA AACATGACAG CTATATCCCC
    TGCTCAGAAA GGATACATGT AGCTGTTACC GAAATGGCAG CATTATTCCC CAAAAAACCC
    AAGTCTGACA CGGTGAGGAC TTCCCTGCGC CTGCTGACAT CCAGTGCCTA CCGACTCCAG
    TCAGAGTGCA AGAAGACCCT CCCCGGGGAC CCCGGCCCGC CCACGGACAT CCAGCTGGTC
    ACACAGCAGG TCATCCAGTG TGCATATGAC ATTGCCAAGG CCGCCAAGCA GCTGGTCACC
    ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963422.1
    CDS136..2082
    Translation

    Target ORF information:

    RefSeq Version XM_014107947.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107947.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGGC CTCCAGGCTA GAAAAGCAGA ACAGCACGCC TGAAAGTGAC
    TACGATAATA CACCCAATGA CACGGACCCA GATGACACAG GGTCAAGCAG AAAGGGAAGG
    CAGAGGAGTA TGGTGTGGCA AGGCGATGGC TCAGTACCAG ACACAGTGGA ACCCCACACT
    GCCCCCAGCC CCATCCTCCC CAGCACGGAA GACGTCATCC GGAAAACTGA ACAGATCACC
    AAAAACATAC AGGAGCTCTT AAGAGCCGCT CAAGAAAATA AACATGACAG CTATATCCCC
    TGCTCAGAAA GGATACATGT AGCTGTTACC GAAATGGCAG CATTATTCCC CAAAAAACCC
    AAGTCTGACA CGGTGAGGAC TTCCCTGCGC CTGCTGACAT CCAGTGCCTA CCGACTCCAG
    TCAGAGTGCA AGAAGACCCT CCCCGGGGAC CCCGGCCCGC CCACGGACAT CCAGCTGGTC
    ACACAGCAGG TCATCCAGTG TGCATATGAC ATTGCCAAGG CCGCCAAGCA GCTGGTCACC
    ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43306
    Clone ID Related Accession (Same CDS sequence) XM_014107947.1 , XM_014107947.2
    Accession Version XM_014107947.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ARF GTPase-activating protein GIT2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107947.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGGC CTCCAGGCTA GAAAAGCAGA ACAGCACGCC TGAAAGTGAC
    TACGATAATA CACCCAATGA CACGGACCCA GATGACACAG GGTCAAGCAG AAAGGGAAGG
    CAGAGGAGTA TGGTGTGGCA AGGCGATGGC TCAGTACCAG ACACAGTGGA ACCCCACACT
    GCCCCCAGCC CCATCCTCCC CAGCACGGAA GACGTCATCC GGAAAACTGA ACAGATCACC
    AAAAACATAC AGGAGCTCTT AAGAGCCGCT CAAGAAAATA AACATGACAG CTATATCCCC
    TGCTCAGAAA GGATACATGT AGCTGTTACC GAAATGGCAG CATTATTCCC CAAAAAACCC
    AAGTCTGACA CGGTGAGGAC TTCCCTGCGC CTGCTGACAT CCAGTGCCTA CCGACTCCAG
    TCAGAGTGCA AGAAGACCCT CCCCGGGGAC CCCGGCCCGC CCACGGACAT CCAGCTGGTC
    ACACAGCAGG TCATCCAGTG TGCATATGAC ATTGCCAAGG CCGCCAAGCA GCTGGTCACC
    ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963422.1
    CDS135..2081
    Translation

    Target ORF information:

    RefSeq Version XM_014107947.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GIT ArfGAP 2 (GIT2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107947.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGAG TCTAGATTCA
    GATTTATCAG ATGGACCAGT CACTGTGCAG GAATTTATGG AGGTCAAAAA CGCTCTAGTG
    GCTTCTGAGG CCAAGATACA GCAGCTAATG AAGGTGAATA ACAACTTGAG TGACGAGCTG
    AGAATTATGC AGAAAAAGGC CTCCAGGCTA GAAAAGCAGA ACAGCACGCC TGAAAGTGAC
    TACGATAATA CACCCAATGA CACGGACCCA GATGACACAG GGTCAAGCAG AAAGGGAAGG
    CAGAGGAGTA TGGTGTGGCA AGGCGATGGC TCAGTACCAG ACACAGTGGA ACCCCACACT
    GCCCCCAGCC CCATCCTCCC CAGCACGGAA GACGTCATCC GGAAAACTGA ACAGATCACC
    AAAAACATAC AGGAGCTCTT AAGAGCCGCT CAAGAAAATA AACATGACAG CTATATCCCC
    TGCTCAGAAA GGATACATGT AGCTGTTACC GAAATGGCAG CATTATTCCC CAAAAAACCC
    AAGTCTGACA CGGTGAGGAC TTCCCTGCGC CTGCTGACAT CCAGTGCCTA CCGACTCCAG
    TCAGAGTGCA AGAAGACCCT CCCCGGGGAC CCCGGCCCGC CCACGGACAT CCAGCTGGTC
    ACACAGCAGG TCATCCAGTG TGCATATGAC ATTGCCAAGG CCGCCAAGCA GCTGGTCACC
    ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43307
    Clone ID Related Accession (Same CDS sequence) XM_014107948.1
    Accession Version XM_014107948.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ARF GTPase-activating protein GIT2 isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGGC CTCCAGGCTA
    GAAAAGCAGA ACAGCACGCC TGAAAGTGAC TACGATAATA CACCCAATGA CACGGACCCA
    GATGACACAG GGTCAAGCAG AAAGGGAAGG CAGAGGAGTA TGGTGTGGCA AGGCGATGGC
    TCAGTACCAG ACACAGTGGA ACCCCACACT GCCCCCAGCC CCATCCTCCC CAGCACGGAA
    GACGTCATCC GGAAAACTGA ACAGATCACC AAAAACATAC AGGAGCTCTT AAGAGCCGCT
    CAAGAAAATA AACATGACAG CTATATCCCC TGCTCAGAAA GGATACATGT AGCTGTTACC
    GAAATGGCAG CATTATTCCC CAAAAAACCC AAGTCTGACA CGGTGAGGAC TTCCCTGCGC
    CTGCTGACAT CCAGTGCCTA CCGACTCCAG TCAGAGTGCA AGAAGACCCT CCCCGGGGAC
    CCCGGCCCGC CCACGGACAT CCAGCTGGTC ACACAGCAGG TCATCCAGTG TGCATATGAC
    ATTGCCAAGG CCGCCAAGCA GCTGGTCACC ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963423.1
    CDS136..1932
    Translation

    Target ORF information:

    RefSeq Version XM_014107948.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107948.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCCAAGC GGCTTCGGAG CAGCGAGGTG TGCGCCGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCAG TAAATAGGGG AACTTTTATA TGTGACGAGT GCTGCAGTGT CCATCGGAGC
    CTAGGGCGCC ACATCTCTCA AGTAAGGCAT CTTAAACACA CACCGTGGCC TCCCACGTTG
    CTGCAGATGG TTGAAACTTT GTATAATAAT GGTGCTAATT CTATATGGGA ACATTCTTTG
    CTGGACCCTG CCTCTATTAT GAGCGGAAGA CGTAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAA TATCAGATGT TAGCATTTGT CCATCGCTTG
    CCTTGCCGGG ATGATGACAG TATGACTGCC AAAGATCTTA GCAAGCAACT TCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTCTTTCACC CTGAAAAAGG AAACACCCCA CTCCACGTCG CCTCCAAAGC AGGGCAGATT
    TTACAGGCAG AATTATTGGC AGTATATGGA GCAGACCCAG GCACACAGGA TTCTAGTGGA
    AAAACTCCTG TGGATTATGC AAGGCAAGGA GGGCACCATG AACTGGCGGA ACGCCTTGTC
    GAAATACAGT ATGAGCTGAC TGACAGACTA GCCTTCTATC TCTGTGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCAG ACAGACGGCT CAGCCTGGAT
    TTGTCTGAAT TGGCAAAAGC TGCTAAGAAG AAACTCCAAT CCCTAAGTAA TCATTTGTTT
    GAAGAACTTG CCATGGATGT GTACGATGAA GTTGACAGGC GAGAGACTGA TGCAGTCTGG
    CTTGCCACGC AAAACCACAG CACCCTGGTA ACCGAGACAA CTGTCGTCCC CTTCCTTCCG
    GTCAATCCTG AGTACTCTTC AACACGGAAC CAGGGCAGAC AGAAACTAGC TCGGTTTAAC
    GCCCATGAGT TTGCCACACT GGTCATCGAC ATTCTCAGTG ACGCCAAGAG GAGACAGCAA
    GGCAGTCCTC TTTCTGGTTC TAAAGACAAT GTGGAGCTGA TACTGAAAAC GATCAGTAAC
    CAGCACAGCA TTGAGAGTCA AGACAATGAC CAGCCAGACT ATGACAGTGT GGCCTCAGAC
    GAAGACACGG ATCTGGAAGC TACTGCAAGC AAAGCAAACA GGCAGAAGGC CTCCAGGCTA
    GAAAAGCAGA ACAGCACGCC TGAAAGTGAC TACGATAATA CACCCAATGA CACGGACCCA
    GATGACACAG GGTCAAGCAG AAAGGGAAGG CAGAGGAGTA TGGTGTGGCA AGGCGATGGC
    TCAGTACCAG ACACAGTGGA ACCCCACACT GCCCCCAGCC CCATCCTCCC CAGCACGGAA
    GACGTCATCC GGAAAACTGA ACAGATCACC AAAAACATAC AGGAGCTCTT AAGAGCCGCT
    CAAGAAAATA AACATGACAG CTATATCCCC TGCTCAGAAA GGATACATGT AGCTGTTACC
    GAAATGGCAG CATTATTCCC CAAAAAACCC AAGTCTGACA CGGTGAGGAC TTCCCTGCGC
    CTGCTGACAT CCAGTGCCTA CCGACTCCAG TCAGAGTGCA AGAAGACCCT CCCCGGGGAC
    CCCGGCCCGC CCACGGACAT CCAGCTGGTC ACACAGCAGG TCATCCAGTG TGCATATGAC
    ATTGCCAAGG CCGCCAAGCA GCTGGTCACC ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.