NOS1 cDNA ORF clone, Canis lupus familiaris(dog)

The following NOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe26020 NM_001195145.1
Latest version!
Canis lupus familiaris nitric oxide synthase 1 (NOS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe26019 XM_014107717.1
Latest version!
Canis lupus familiaris nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe26019 XM_005636015.3
Latest version!
Canis lupus familiaris nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe26019 XM_014107718.1
Latest version!
Canis lupus familiaris nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe26019 XM_014107715.2
Latest version!
Canis lupus familiaris nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe26019 XM_014107719.1
Latest version!
Canis lupus familiaris nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe26019 XM_014107716.1
Latest version!
Canis lupus familiaris nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe26020 XM_005636016.2
Latest version!
Canis lupus familiaris nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe26019 XM_014107715.1 Canis lupus familiaris nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe26019 XM_005636015.2 Canis lupus familiaris nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe26020
    Clone ID Related Accession (Same CDS sequence) XM_005636016.2 , NM_001195145.1
    Accession Version NM_001195145.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Canis lupus familiaris(dog)
    Product nitric oxide synthase, brain
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03014715.1. On Aug 25, 2010 this sequence version replaced XM_534695.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2240594 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGAGCTACA AAGTCCGGTT CAATAGTGTT TCCTCATATT CTGATTCTCA TAAGTCATCG
    GGTGATGGGC CAGACCTCAG AGACAACTTT GAGAGTGCTG GACCCCTGGC AAATGTGAGG
    TTCTCTGTGT TTGGCCTTGG CTCCCGGGCT TACCCTCACT TTTGCGCCTT TGGACATGCT
    GTGGACACCC TCCTGGAGGA GCTGGGAGGA GAGAGAATCC TGAAGATGAG GGAAGGGGAT
    GAGCTCTGTG GACAGGAAGA GGCTTTCAGG ACTTGGGCCA AGAAGGTCTT CAAGGCAGCC
    TGTGATGTGT TCTGCGTGGG AGATGATGTC AACATTGAAA AGGCCAACAA CTCCCTCATC
    AGCAATGACC GCAGCTGGAA GAGAAACAAG TTCCGCCTCA CCTATGTGGC CGAAGCTCCA
    GAACTTACAC AGGGTCTATC CAACGTCCAC AAAAAGCGGG TCTCAGCCGC TCGACTCCTC
    AGCCGTCAAA ACCTTCAGAG TCCTAAATCC AGCCGATCAA CCATCTTCGT GCGTCTCCAC
    ACCAATGGGA ATCAGGAGCT GCAGTACCAG CCTGGGGACC ACCTGGGCGT CTTCCCTGGC
    AACCGCGAGG ACCTTGTGAA TGCCCTGATG GAGCGGCTAG AGGATGCACC CCCCGTCAAC
    CAGCTGGTGA AGGTGGAGCT ACTGGAGGAG CGCAACACGG CTCTGGGAGT TATCAGTAAC
    TGGACGGATG AGCTCCGCCT CCCACCCTGC ACCATCTTCC AGGCCTTCAA GTACTACCTG
    GACATCACCA CGCCGCCAAC GCCACTGCAG CTGCAACAGT TTGCCTCCCT GGCCACCAGC
    GAGAAAGAGA AGCAGCGTCT ACTGGTCCTC AGCAAGGGTT TACAGGAGTA CGAGGAATGG
    AAATGGGGTA AGAACCCCAC CATCGTGGAG GTGCTGGAGG AGTTCCCGTC CATCCAGATG
    CCTTCCACCC TGCTCCTAAC CCAGCTGTCG CTGCTGCAGC CCCGCTACTA CTCCATCAGC
    TCCTCCCCAG ACATGTATCC AGATGAGGTG CACCTCACCG TGGCAATTGT CTCCTATCGC
    ACCGGAGATG GAGAAGGACC AATTCACCAC GGCGTGTGCT CCTCCTGGCT CAACCGGATA
    CAGGCTGACG AAGTGGTCCC ATGTTTCGTG AGAGGAGCAC CCAGCTTCCA CCTGCCCCGA
    AATCCCCAAG TGCCCTGTAT CCTCATTGGC CCAGGCACTG GCATCGCCCC TTTCCGAAGC
    TTCTGGCAGC AGCGGCAATT TGATATCCAA CACAAAGGAA TGAGTCCGTG CCCCATGGTC
    CTGGTGTTCG GGTGCCGGCA GTCCAAGATA GACCACATCT ACAGGGAGGA GACTCTACAG
    GCCAAGAACA AGGGGGTCTT CAGAGAACTG TACACGGCCT ACTCCCGGGA GCCAGACAAA
    CCAAAGAAGT ATGTGCAGGA CATCCTGCAG GAGCAGCTAG CAGAACCTGT GTACCGAGCC
    CTGAAGGAGC AAGGGGGCCA CATCTATGTC TGCGGGGACG TCACCATGGC CGCCGATGTC
    CTCAAAGCCA TCCAGCGCAT CATGACCCAG CAGGGGAAAC TCTCTGTAGA GGACGCGGGC
    GTGTTCATCA GCAGGCTGAG GGATGACAAC CGGTACCATG AGGATATTTT CGGGGTCACC
    CTCCGAACGT ATGAAGTGAC CAACCGCCTT AGATCTGAAT CCATTGCCTT CATTGAAGAG
    AGCAAAAAAG ACACCGATGA GGTTTTCAGC TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001182074.1
    CDS1..4296
    Translation

    Target ORF information:

    RefSeq Version NM_001195145.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nitric oxide synthase 1 (NOS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001195145.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGAGCTACA AAGTCCGGTT CAATAGTGTT TCCTCATATT CTGATTCTCA TAAGTCATCG
    GGTGATGGGC CAGACCTCAG AGACAACTTT GAGAGTGCTG GACCCCTGGC AAATGTGAGG
    TTCTCTGTGT TTGGCCTTGG CTCCCGGGCT TACCCTCACT TTTGCGCCTT TGGACATGCT
    GTGGACACCC TCCTGGAGGA GCTGGGAGGA GAGAGAATCC TGAAGATGAG GGAAGGGGAT
    GAGCTCTGTG GACAGGAAGA GGCTTTCAGG ACTTGGGCCA AGAAGGTCTT CAAGGCAGCC
    TGTGATGTGT TCTGCGTGGG AGATGATGTC AACATTGAAA AGGCCAACAA CTCCCTCATC
    AGCAATGACC GCAGCTGGAA GAGAAACAAG TTCCGCCTCA CCTATGTGGC CGAAGCTCCA
    GAACTTACAC AGGGTCTATC CAACGTCCAC AAAAAGCGGG TCTCAGCCGC TCGACTCCTC
    AGCCGTCAAA ACCTTCAGAG TCCTAAATCC AGCCGATCAA CCATCTTCGT GCGTCTCCAC
    ACCAATGGGA ATCAGGAGCT GCAGTACCAG CCTGGGGACC ACCTGGGCGT CTTCCCTGGC
    AACCGCGAGG ACCTTGTGAA TGCCCTGATG GAGCGGCTAG AGGATGCACC CCCCGTCAAC
    CAGCTGGTGA AGGTGGAGCT ACTGGAGGAG CGCAACACGG CTCTGGGAGT TATCAGTAAC
    TGGACGGATG AGCTCCGCCT CCCACCCTGC ACCATCTTCC AGGCCTTCAA GTACTACCTG
    GACATCACCA CGCCGCCAAC GCCACTGCAG CTGCAACAGT TTGCCTCCCT GGCCACCAGC
    GAGAAAGAGA AGCAGCGTCT ACTGGTCCTC AGCAAGGGTT TACAGGAGTA CGAGGAATGG
    AAATGGGGTA AGAACCCCAC CATCGTGGAG GTGCTGGAGG AGTTCCCGTC CATCCAGATG
    CCTTCCACCC TGCTCCTAAC CCAGCTGTCG CTGCTGCAGC CCCGCTACTA CTCCATCAGC
    TCCTCCCCAG ACATGTATCC AGATGAGGTG CACCTCACCG TGGCAATTGT CTCCTATCGC
    ACCGGAGATG GAGAAGGACC AATTCACCAC GGCGTGTGCT CCTCCTGGCT CAACCGGATA
    CAGGCTGACG AAGTGGTCCC ATGTTTCGTG AGAGGAGCAC CCAGCTTCCA CCTGCCCCGA
    AATCCCCAAG TGCCCTGTAT CCTCATTGGC CCAGGCACTG GCATCGCCCC TTTCCGAAGC
    TTCTGGCAGC AGCGGCAATT TGATATCCAA CACAAAGGAA TGAGTCCGTG CCCCATGGTC
    CTGGTGTTCG GGTGCCGGCA GTCCAAGATA GACCACATCT ACAGGGAGGA GACTCTACAG
    GCCAAGAACA AGGGGGTCTT CAGAGAACTG TACACGGCCT ACTCCCGGGA GCCAGACAAA
    CCAAAGAAGT ATGTGCAGGA CATCCTGCAG GAGCAGCTAG CAGAACCTGT GTACCGAGCC
    CTGAAGGAGC AAGGGGGCCA CATCTATGTC TGCGGGGACG TCACCATGGC CGCCGATGTC
    CTCAAAGCCA TCCAGCGCAT CATGACCCAG CAGGGGAAAC TCTCTGTAGA GGACGCGGGC
    GTGTTCATCA GCAGGCTGAG GGATGACAAC CGGTACCATG AGGATATTTT CGGGGTCACC
    CTCCGAACGT ATGAAGTGAC CAACCGCCTT AGATCTGAAT CCATTGCCTT CATTGAAGAG
    AGCAAAAAAG ACACCGATGA GGTTTTCAGC TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26019
    Clone ID Related Accession (Same CDS sequence) XM_014107715.2 , XM_005636015.2 , XM_014107718.1 , XM_014107716.1 , XM_014107717.1 , XM_014107715.1 , XM_014107719.1 , XM_005636015.3
    Accession Version XM_014107715.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107715.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963190.1
    CDS769..5166
    Translation

    Target ORF information:

    RefSeq Version XM_014107715.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107715.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26019
    Clone ID Related Accession (Same CDS sequence) XM_014107715.2 , XM_005636015.2 , XM_014107718.1 , XM_014107716.1 , XM_014107717.1 , XM_014107715.1 , XM_014107719.1 , XM_005636015.3
    Accession Version XM_005636015.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nitric oxide synthase, brain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636072.1
    CDS464..4861
    Translation

    Target ORF information:

    RefSeq Version XM_005636015.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636015.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26019
    Clone ID Related Accession (Same CDS sequence) XM_014107715.2 , XM_005636015.2 , XM_014107718.1 , XM_014107716.1 , XM_014107717.1 , XM_014107715.1 , XM_014107719.1 , XM_005636015.3
    Accession Version XM_014107718.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nitric oxide synthase, brain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963193.1
    CDS444..4841
    Translation

    Target ORF information:

    RefSeq Version XM_014107718.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107718.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26019
    Clone ID Related Accession (Same CDS sequence) XM_014107715.2 , XM_005636015.2 , XM_014107718.1 , XM_014107716.1 , XM_014107717.1 , XM_014107715.1 , XM_014107719.1 , XM_005636015.3
    Accession Version XM_014107716.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nitric oxide synthase, brain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963191.1
    CDS472..4869
    Translation

    Target ORF information:

    RefSeq Version XM_014107716.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107716.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26019
    Clone ID Related Accession (Same CDS sequence) XM_014107715.2 , XM_005636015.2 , XM_014107718.1 , XM_014107716.1 , XM_014107717.1 , XM_014107715.1 , XM_014107719.1 , XM_005636015.3
    Accession Version XM_014107717.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nitric oxide synthase, brain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963192.1
    CDS459..4856
    Translation

    Target ORF information:

    RefSeq Version XM_014107717.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107717.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26019
    Clone ID Related Accession (Same CDS sequence) XM_014107715.2 , XM_005636015.2 , XM_014107718.1 , XM_014107716.1 , XM_014107717.1 , XM_014107715.1 , XM_014107719.1 , XM_005636015.3
    Accession Version XM_014107715.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nitric oxide synthase, brain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963190.1
    CDS770..5167
    Translation

    Target ORF information:

    RefSeq Version XM_014107715.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107715.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26019
    Clone ID Related Accession (Same CDS sequence) XM_014107715.2 , XM_005636015.2 , XM_014107718.1 , XM_014107716.1 , XM_014107717.1 , XM_014107715.1 , XM_014107719.1 , XM_005636015.3
    Accession Version XM_014107719.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nitric oxide synthase, brain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963194.1
    CDS430..4827
    Translation

    Target ORF information:

    RefSeq Version XM_014107719.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107719.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26019
    Clone ID Related Accession (Same CDS sequence) XM_014107715.2 , XM_005636015.2 , XM_014107718.1 , XM_014107716.1 , XM_014107717.1 , XM_014107715.1 , XM_014107719.1 , XM_005636015.3
    Accession Version XM_005636015.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636015.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636072.1
    CDS479..4876
    Translation

    Target ORF information:

    RefSeq Version XM_005636015.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636015.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGTACCCGG AACCCTTGCG TTTCTTTCCC CGTAAAGGGC CTCCCCTCCC CCAAGGTGAC
    ACAGAAGTCC ATGGTCTGGC TGCAGTTCGT GACAGCCAGC AGAGGAGCTA CAAAGTCCGG
    TTCAATAGTG TTTCCTCATA TTCTGATTCT CATAAGTCAT CGGGTGATGG GCCAGACCTC
    AGAGACAACT TTGAGAGTGC TGGACCCCTG GCAAATGTGA GGTTCTCTGT GTTTGGCCTT
    GGCTCCCGGG CTTACCCTCA CTTTTGCGCC TTTGGACATG CTGTGGACAC CCTCCTGGAG
    GAGCTGGGAG GAGAGAGAAT CCTGAAGATG AGGGAAGGGG ATGAGCTCTG TGGACAGGAA
    GAGGCTTTCA GGACTTGGGC CAAGAAGGTC TTCAAGGCAG CCTGTGATGT GTTCTGCGTG
    GGAGATGATG TCAACATTGA AAAGGCCAAC AACTCCCTCA TCAGCAATGA CCGCAGCTGG
    AAGAGAAACA AGTTCCGCCT CACCTATGTG GCCGAAGCTC CAGAACTTAC ACAGGGTCTA
    TCCAACGTCC ACAAAAAGCG GGTCTCAGCC GCTCGACTCC TCAGCCGTCA AAACCTTCAG
    AGTCCTAAAT CCAGCCGATC AACCATCTTC GTGCGTCTCC ACACCAATGG GAATCAGGAG
    CTGCAGTACC AGCCTGGGGA CCACCTGGGC GTCTTCCCTG GCAACCGCGA GGACCTTGTG
    AATGCCCTGA TGGAGCGGCT AGAGGATGCA CCCCCCGTCA ACCAGCTGGT GAAGGTGGAG
    CTACTGGAGG AGCGCAACAC GGCTCTGGGA GTTATCAGTA ACTGGACGGA TGAGCTCCGC
    CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC TGGACATCAC CACGCCGCCA
    ACGCCACTGC AGCTGCAACA GTTTGCCTCC CTGGCCACCA GCGAGAAAGA GAAGCAGCGT
    CTACTGGTCC TCAGCAAGGG TTTACAGGAG TACGAGGAAT GGAAATGGGG TAAGAACCCC
    ACCATCGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA TGCCTTCCAC CCTGCTCCTA
    ACCCAGCTGT CGCTGCTGCA GCCCCGCTAC TACTCCATCA GCTCCTCCCC AGACATGTAT
    CCAGATGAGG TGCACCTCAC CGTGGCAATT GTCTCCTATC GCACCGGAGA TGGAGAAGGA
    CCAATTCACC ACGGCGTGTG CTCCTCCTGG CTCAACCGGA TACAGGCTGA CGAAGTGGTC
    CCATGTTTCG TGAGAGGAGC ACCCAGCTTC CACCTGCCCC GAAATCCCCA AGTGCCCTGT
    ATCCTCATTG GCCCAGGCAC TGGCATCGCC CCTTTCCGAA GCTTCTGGCA GCAGCGGCAA
    TTTGATATCC AACACAAAGG AATGAGTCCG TGCCCCATGG TCCTGGTGTT CGGGTGCCGG
    CAGTCCAAGA TAGACCACAT CTACAGGGAG GAGACTCTAC AGGCCAAGAA CAAGGGGGTC
    TTCAGAGAAC TGTACACGGC CTACTCCCGG GAGCCAGACA AACCAAAGAA GTATGTGCAG
    GACATCCTGC AGGAGCAGCT AGCAGAACCT GTGTACCGAG CCCTGAAGGA GCAAGGGGGC
    CACATCTATG TCTGCGGGGA CGTCACCATG GCCGCCGATG TCCTCAAAGC CATCCAGCGC
    ATCATGACCC AGCAGGGGAA ACTCTCTGTA GAGGACGCGG GCGTGTTCAT CAGCAGGCTG
    AGGGATGACA ACCGGTACCA TGAGGATATT TTCGGGGTCA CCCTCCGAAC GTATGAAGTG
    ACCAACCGCC TTAGATCTGA ATCCATTGCC TTCATTGAAG AGAGCAAAAA AGACACCGAT
    GAGGTTTTCA GCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26020
    Clone ID Related Accession (Same CDS sequence) XM_005636016.2 , NM_001195145.1
    Accession Version XM_005636016.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nitric oxide synthase, brain isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGAGCTACA AAGTCCGGTT CAATAGTGTT TCCTCATATT CTGATTCTCA TAAGTCATCG
    GGTGATGGGC CAGACCTCAG AGACAACTTT GAGAGTGCTG GACCCCTGGC AAATGTGAGG
    TTCTCTGTGT TTGGCCTTGG CTCCCGGGCT TACCCTCACT TTTGCGCCTT TGGACATGCT
    GTGGACACCC TCCTGGAGGA GCTGGGAGGA GAGAGAATCC TGAAGATGAG GGAAGGGGAT
    GAGCTCTGTG GACAGGAAGA GGCTTTCAGG ACTTGGGCCA AGAAGGTCTT CAAGGCAGCC
    TGTGATGTGT TCTGCGTGGG AGATGATGTC AACATTGAAA AGGCCAACAA CTCCCTCATC
    AGCAATGACC GCAGCTGGAA GAGAAACAAG TTCCGCCTCA CCTATGTGGC CGAAGCTCCA
    GAACTTACAC AGGGTCTATC CAACGTCCAC AAAAAGCGGG TCTCAGCCGC TCGACTCCTC
    AGCCGTCAAA ACCTTCAGAG TCCTAAATCC AGCCGATCAA CCATCTTCGT GCGTCTCCAC
    ACCAATGGGA ATCAGGAGCT GCAGTACCAG CCTGGGGACC ACCTGGGCGT CTTCCCTGGC
    AACCGCGAGG ACCTTGTGAA TGCCCTGATG GAGCGGCTAG AGGATGCACC CCCCGTCAAC
    CAGCTGGTGA AGGTGGAGCT ACTGGAGGAG CGCAACACGG CTCTGGGAGT TATCAGTAAC
    TGGACGGATG AGCTCCGCCT CCCACCCTGC ACCATCTTCC AGGCCTTCAA GTACTACCTG
    GACATCACCA CGCCGCCAAC GCCACTGCAG CTGCAACAGT TTGCCTCCCT GGCCACCAGC
    GAGAAAGAGA AGCAGCGTCT ACTGGTCCTC AGCAAGGGTT TACAGGAGTA CGAGGAATGG
    AAATGGGGTA AGAACCCCAC CATCGTGGAG GTGCTGGAGG AGTTCCCGTC CATCCAGATG
    CCTTCCACCC TGCTCCTAAC CCAGCTGTCG CTGCTGCAGC CCCGCTACTA CTCCATCAGC
    TCCTCCCCAG ACATGTATCC AGATGAGGTG CACCTCACCG TGGCAATTGT CTCCTATCGC
    ACCGGAGATG GAGAAGGACC AATTCACCAC GGCGTGTGCT CCTCCTGGCT CAACCGGATA
    CAGGCTGACG AAGTGGTCCC ATGTTTCGTG AGAGGAGCAC CCAGCTTCCA CCTGCCCCGA
    AATCCCCAAG TGCCCTGTAT CCTCATTGGC CCAGGCACTG GCATCGCCCC TTTCCGAAGC
    TTCTGGCAGC AGCGGCAATT TGATATCCAA CACAAAGGAA TGAGTCCGTG CCCCATGGTC
    CTGGTGTTCG GGTGCCGGCA GTCCAAGATA GACCACATCT ACAGGGAGGA GACTCTACAG
    GCCAAGAACA AGGGGGTCTT CAGAGAACTG TACACGGCCT ACTCCCGGGA GCCAGACAAA
    CCAAAGAAGT ATGTGCAGGA CATCCTGCAG GAGCAGCTAG CAGAACCTGT GTACCGAGCC
    CTGAAGGAGC AAGGGGGCCA CATCTATGTC TGCGGGGACG TCACCATGGC CGCCGATGTC
    CTCAAAGCCA TCCAGCGCAT CATGACCCAG CAGGGGAAAC TCTCTGTAGA GGACGCGGGC
    GTGTTCATCA GCAGGCTGAG GGATGACAAC CGGTACCATG AGGATATTTT CGGGGTCACC
    CTCCGAACGT ATGAAGTGAC CAACCGCCTT AGATCTGAAT CCATTGCCTT CATTGAAGAG
    AGCAAAAAAG ACACCGATGA GGTTTTCAGC TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636073.1
    CDS464..4759
    Translation

    Target ORF information:

    RefSeq Version XM_005636016.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636016.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGAGTC AGGTGTTCAG TGTTCAGCAA ATCCAACCCA ACGTCATTTC TGTCCGCCTC 
    TTCAAGCGCA AGGTGGGGGG TCTGGGCTTC CTGGTGAAGG AGCGAGTGAG CAAGCCGCCC
    GTGATCATCT CTGACCTGAT TCGGGGGGGT GCTGCGGAGC AGAGCGGCCT CATCCAGGCC
    GGCGACATCA TCCTCGCAGT CAACGGCCAG CCCTTGGTGG ACCTGAACTA TGACAGTGCC
    CTGGAGGTGC TCAGGGGCAT TGCCTCTGAG ACACATGTAG TCCTCATTCT GAGGGGCCCT
    GAAGGCTTCA CCACTCACCT GGAGACCACT TTCACTGGGG ATGGGACCCC CAAGACCATC
    CGGGTAACAC GGCCCCTGGG TCCCCCCACC ACAGCTGTGG ATCTGTCTCA CCAGTCATCC
    GCCAGCAAAG AGCACACACC GGACGGGGCC ACAGGTCCTG GCAATGGGCC ACAGCATATC
    CAAGACAGTG GCCAGCAAGC CAGCTCACTC TTGAATACCA ACAGCCTGGC TCCCAGACCC
    CCGAGCCAGG ACTCTACCAA GAAGAGTACC GGGGTTGCTC TCCAGGACAG CCAGAACAAT
    GAATTGCTCA AAGAGATAGA GCCTGTGCTG AGTCTTCTCA CCAGTGGAAA CAAAGGGATC
    AATGGAGTGG GACCTTCCAA AGCAGAGACA AAAGATGCAG AAGTCCAGGT GGACAGAGGT
    ATGGACAGCA AGTCACACAA ACCACTGCCC CTTGGCGTGG AGAATGACCG AGTCTTCAGT
    GACCTGTGGG GGAAGGGCCA TGTGCCTGTC GTCCTCAACA ACCCGTATTC AGAGAAGGAG
    CAGCCCCCTG CCTCGGGAAA ACAGTCTCCC ACAAAGAATG GAAGCCCCTC CAAGTGCCCC
    CGCTTCCTCA AGGTCAAGAA CTGGGAGACG GATGTGGTTC TCACTGACAC CCTCCACCTT
    AAGAGCACAT TGGAAACGGG ATGCACCGAG CACATCTGTA TGGGCTCCAT CATGCTCCCT
    TCTCAGCAAA TACGAAGGCC TGAAGACGTC CGCACAAAAG AACAGCTCTT TCCTCTCGCC
    AAAGAGTTCA TTGATCAGTA CTACTCGTCA ATTAAAAGAT CTGGCTCCAA AGCCCACACA
    GAGAGGCTGG AAGAGGTGAA CAAAGAGATC GAAACCACAG GCACCTACCA GCTCAAAGAC
    ACGGAGCTCA TCTATGGGGC AAAGCACGCC TGGCGGAATG CCTCCCGCTG CGTCGGCAGG
    ATCCAGTGGT CCAAGCTGCA GGTGTTCGAT GCCCGTGACT GCACCACAGC TCATGGGATG
    TTCAACTACA TCTGCAACCA CATCAAGTAT GCCACCAACA AAGGGAACCT CCGGTCTGCC
    ATCACCATAT TTCCCCAGAG GACTGACGGC AAGCACGATT TCCGAGTCTG GAACTCTCAG
    CTCATCCGCT ATGCCGGCTA CAAGCAGCCC GACGGTTCCA TCCTAGGGGA CCCAGCCAAC
    GTGGAATTCA CAGAGATCTG CACACAGCAG GGCTGGAAAC CCCCAAGAGG CCGCTTCGAT
    GTCCTGCCAC TCCTGCTCCA GGCCAACGGC AACGACCCCG AGCTCTTCCA GATTCCTCCA
    GAGCTGGTGT TGGAAGTACC CATTAGGCAC CCCAAGTTTG AGTGGTTCAA GGATCTGGGC
    CTGAAGTGGT ACGGCCTCCC CGCTGTGTCC AACATGCTGC TGGAGATCGG CGGCCTGGAG
    TTCAGCGCCT GTCCCTTCAG CGGCTGGTAC ATGGGCACAG AGATTGGCGT CCGCGACTAC
    TGTGACAACT CTCGATACAA CATCCTGGAG GAAGTGGCCA AGAAGATGAA CTTGGACATG
    AGGAAGACAT CCTCCCTGTG GAAGGACCAG GCACTGGTGG AAATTAACAT TGCTGTCCTC
    TATAGCTTCC AGAGTGACAA AGTTACCATC GTTGACCACC ACTCTGCCAC CGAGTCCTTC
    ATTAAGCACA TGGAGAATGA ATACCGCTGC CGAGGGGGCT GCCCTGCTGA CTGGGTGTGG
    ATTGTGCCCC CCATGTCTGG CAGCATCACC CCTGTCTTCC ACCAGGAGAT GCTCAACTAC
    CGGCTTACCC CCTCCTTCGA ATACCAGCCG GAGCCTTGGA ACACCCATGT CTGGAAAGGT
    ACCAATGGGA CCCCCACAAA GCGGCGAGCC ATCGGTTTCA AGAAGCTGGC AGAAGCCGTC
    AAGTTCTCGG CCAAGCTCAT GGGGCAAGCA ATGGCCAAGA GGGTCAAGGC GACCATCCTT
    TATGCCACTG AGACGGGCAA GTCACAGGCC TATGCTAAAA CCTTGTGTGA GATCTTCAAA
    CATGCTTTTG ATGCCAAGGT GATGTCCATG GAAGAATATG ACATTGTACA CCTGGAGCAT
    GAAACTCTGG TCCTGGTGGT TACCAGCACT TTTGGCAATG GAGACCCACC TGAGAATGGG
    GAGAAATTCG GCTGTGCTTT GATGGAAATG AGGCATCCCA ACTCTGTGCA TGAGGAAAGG
    AAGAGCTACA AAGTCCGGTT CAATAGTGTT TCCTCATATT CTGATTCTCA TAAGTCATCG
    GGTGATGGGC CAGACCTCAG AGACAACTTT GAGAGTGCTG GACCCCTGGC AAATGTGAGG
    TTCTCTGTGT TTGGCCTTGG CTCCCGGGCT TACCCTCACT TTTGCGCCTT TGGACATGCT
    GTGGACACCC TCCTGGAGGA GCTGGGAGGA GAGAGAATCC TGAAGATGAG GGAAGGGGAT
    GAGCTCTGTG GACAGGAAGA GGCTTTCAGG ACTTGGGCCA AGAAGGTCTT CAAGGCAGCC
    TGTGATGTGT TCTGCGTGGG AGATGATGTC AACATTGAAA AGGCCAACAA CTCCCTCATC
    AGCAATGACC GCAGCTGGAA GAGAAACAAG TTCCGCCTCA CCTATGTGGC CGAAGCTCCA
    GAACTTACAC AGGGTCTATC CAACGTCCAC AAAAAGCGGG TCTCAGCCGC TCGACTCCTC
    AGCCGTCAAA ACCTTCAGAG TCCTAAATCC AGCCGATCAA CCATCTTCGT GCGTCTCCAC
    ACCAATGGGA ATCAGGAGCT GCAGTACCAG CCTGGGGACC ACCTGGGCGT CTTCCCTGGC
    AACCGCGAGG ACCTTGTGAA TGCCCTGATG GAGCGGCTAG AGGATGCACC CCCCGTCAAC
    CAGCTGGTGA AGGTGGAGCT ACTGGAGGAG CGCAACACGG CTCTGGGAGT TATCAGTAAC
    TGGACGGATG AGCTCCGCCT CCCACCCTGC ACCATCTTCC AGGCCTTCAA GTACTACCTG
    GACATCACCA CGCCGCCAAC GCCACTGCAG CTGCAACAGT TTGCCTCCCT GGCCACCAGC
    GAGAAAGAGA AGCAGCGTCT ACTGGTCCTC AGCAAGGGTT TACAGGAGTA CGAGGAATGG
    AAATGGGGTA AGAACCCCAC CATCGTGGAG GTGCTGGAGG AGTTCCCGTC CATCCAGATG
    CCTTCCACCC TGCTCCTAAC CCAGCTGTCG CTGCTGCAGC CCCGCTACTA CTCCATCAGC
    TCCTCCCCAG ACATGTATCC AGATGAGGTG CACCTCACCG TGGCAATTGT CTCCTATCGC
    ACCGGAGATG GAGAAGGACC AATTCACCAC GGCGTGTGCT CCTCCTGGCT CAACCGGATA
    CAGGCTGACG AAGTGGTCCC ATGTTTCGTG AGAGGAGCAC CCAGCTTCCA CCTGCCCCGA
    AATCCCCAAG TGCCCTGTAT CCTCATTGGC CCAGGCACTG GCATCGCCCC TTTCCGAAGC
    TTCTGGCAGC AGCGGCAATT TGATATCCAA CACAAAGGAA TGAGTCCGTG CCCCATGGTC
    CTGGTGTTCG GGTGCCGGCA GTCCAAGATA GACCACATCT ACAGGGAGGA GACTCTACAG
    GCCAAGAACA AGGGGGTCTT CAGAGAACTG TACACGGCCT ACTCCCGGGA GCCAGACAAA
    CCAAAGAAGT ATGTGCAGGA CATCCTGCAG GAGCAGCTAG CAGAACCTGT GTACCGAGCC
    CTGAAGGAGC AAGGGGGCCA CATCTATGTC TGCGGGGACG TCACCATGGC CGCCGATGTC
    CTCAAAGCCA TCCAGCGCAT CATGACCCAG CAGGGGAAAC TCTCTGTAGA GGACGCGGGC
    GTGTTCATCA GCAGGCTGAG GGATGACAAC CGGTACCATG AGGATATTTT CGGGGTCACC
    CTCCGAACGT ATGAAGTGAC CAACCGCCTT AGATCTGAAT CCATTGCCTT CATTGAAGAG
    AGCAAAAAAG ACACCGATGA GGTTTTCAGC TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.