ITCH gene cDNA ORF clone, Canis lupus familiaris(Dog)

mRNA	Protein	Name
XM_022409130.1	XP_022264838.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
XM_005634950.3	XP_005635007.1	E3 ubiquitin-protein ligase Itchy homolog isoform X2
XM_022409131.1	XP_022264839.1	E3 ubiquitin-protein ligase Itchy homolog isoform X3
XM_005634950.2	XP_005635007.1	E3 ubiquitin-protein ligase Itchy homolog isoform X2
XM_022409132.1	XP_022264840.1	E3 ubiquitin-protein ligase Itchy homolog isoform X4
XM_014107005.1	XP_013962480.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
XM_014107005.2	XP_013962480.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
XM_846367.5	XP_851460.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
XM_846367.4	XP_851460.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe24762	XM_022409130.1 Latest version!	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe24762	XM_846367.5 Latest version!	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe24762	XM_014107005.2 Latest version!	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe24763	XM_005634950.3 Latest version!	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe296788	XM_022409131.1 Latest version!	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe296789	XM_022409132.1 Latest version!	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OCe24763	XM_005634950.2	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe24762	XM_014107005.1	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe24762	XM_846367.4	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ITCH ( XM_022409130.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022264838.1 Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe24762
Clone ID Related Accession (Same CDS sequence)	XM_022409130.1 , XM_014107005.1 , XM_014107005.2 , XM_846367.5 , XM_846367.4
Accession Version	XM_022409130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCA GTGGATCGCA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGC 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATCGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATCTTTG AAACCTTAAA GTCAAACAAC ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCTGT CTGTCTTGAT 
GGGCTGCAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGTT 
CCACAGAATG ATGATGGCTC TAGACCGAAG GATGAAACAA GAACAAACAC AAATGGATCA 
GATGAGCCTG AGGATACTGG GACTGGTGAA AATAGGAAGG TCAATGGAAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCTGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGATCCAGTA CAGGCTCTCT GCCACCAACA AATATAAATT CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTACCTC AGGCTCCCCT ACCACCTGGT TGGGAACAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCGGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ACTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCGACATTG GAATCTGTCC GGAATTATGA ACAGTGGCAG 
CTCCAACGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT TATTTATGGG 
AATCAAGATT TGTTTACTAC ATCACAAAAT AAAGAATTTG ATCCCCTTGG TCCCCTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAGT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACAGTTGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATTGA TCCCCGCACT GGAAAATCTG CACTAGACAA TGGACCACAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAA GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACGTTGTT TGAAGATTCC 
TTTCAGCAGA TAATGAGCTT CAATCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA 
CACGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATCA ATCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGAAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATATT GAACAAACCA GTTGGACTGA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA ATGTGGTTTG 
GAAATGTACT TCTCTGTCGA TAAAGAAATT TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCTAATGGTG GCAATATTCT TGTGACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GGTTGTCTCG AGGTGTTGAA GAACAAACAC AAGCTTTCTT TGAGGGCTTT 
AATGAAATCC TTCCCCAGCA GTATTTGCAA TACTTTGACG CAAAGGAATT AGAGGTCCTT 
TTATGCGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TACACTAGGA CAAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGACCA CAAAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGT TGCCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AGCTGAAGGA AAAGCTTTTA TTTGCCATTG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264838.1
CDS	123..2711
Translation	MSGSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIFETLKSNNMKLEEVVVTLQLVGDKEPTETIGDLSVCLDGLQLESEVVTNGETTCSESVPQNDDGSRPKDETRTNTNGSDEPEDTGTGENRKVNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNINSNTSEGATSGLIIPLTISGGSGPRPLNPVPQAPLPPGWEQRVDQHGRVYYVDHIEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFTTSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFNPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_022409130.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409130.1

ORF Insert Sequence:

ATGTCTGGCA GTGGATCGCA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGC 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATCGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATCTTTG AAACCTTAAA GTCAAACAAC ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCTGT CTGTCTTGAT 
GGGCTGCAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGTT 
CCACAGAATG ATGATGGCTC TAGACCGAAG GATGAAACAA GAACAAACAC AAATGGATCA 
GATGAGCCTG AGGATACTGG GACTGGTGAA AATAGGAAGG TCAATGGAAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCTGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGATCCAGTA CAGGCTCTCT GCCACCAACA AATATAAATT CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTACCTC AGGCTCCCCT ACCACCTGGT TGGGAACAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCGGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ACTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCGACATTG GAATCTGTCC GGAATTATGA ACAGTGGCAG 
CTCCAACGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT TATTTATGGG 
AATCAAGATT TGTTTACTAC ATCACAAAAT AAAGAATTTG ATCCCCTTGG TCCCCTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAGT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACAGTTGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATTGA TCCCCGCACT GGAAAATCTG CACTAGACAA TGGACCACAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAA GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACGTTGTT TGAAGATTCC 
TTTCAGCAGA TAATGAGCTT CAATCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA 
CACGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATCA ATCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGAAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATATT GAACAAACCA GTTGGACTGA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA ATGTGGTTTG 
GAAATGTACT TCTCTGTCGA TAAAGAAATT TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCTAATGGTG GCAATATTCT TGTGACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GGTTGTCTCG AGGTGTTGAA GAACAAACAC AAGCTTTCTT TGAGGGCTTT 
AATGAAATCC TTCCCCAGCA GTATTTGCAA TACTTTGACG CAAAGGAATT AGAGGTCCTT 
TTATGCGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TACACTAGGA CAAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGACCA CAAAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGT TGCCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AGCTGAAGGA AAAGCTTTTA TTTGCCATTG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_014107005.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962480.1 Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe24762
Clone ID Related Accession (Same CDS sequence)	XM_022409130.1 , XM_014107005.1 , XM_014107005.2 , XM_846367.5 , XM_846367.4
Accession Version	XM_014107005.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCA GTGGATCGCA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGC 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATCGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATCTTTG AAACCTTAAA GTCAAACAAC ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCTGT CTGTCTTGAT 
GGGCTGCAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGTT 
CCACAGAATG ATGATGGCTC TAGACCGAAG GATGAAACAA GAACAAACAC AAATGGATCA 
GATGAGCCTG AGGATACTGG GACTGGTGAA AATAGGAAGG TCAATGGAAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCTGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGATCCAGTA CAGGCTCTCT GCCACCAACA AATATAAATT CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTACCTC AGGCTCCCCT ACCACCTGGT TGGGAACAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCGGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ACTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCGACATTG GAATCTGTCC GGAATTATGA ACAGTGGCAG 
CTCCAACGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT TATTTATGGG 
AATCAAGATT TGTTTACTAC ATCACAAAAT AAAGAATTTG ATCCCCTTGG TCCCCTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAGT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACAGTTGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATTGA TCCCCGCACT GGAAAATCTG CACTAGACAA TGGACCACAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAA GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACGTTGTT TGAAGATTCC 
TTTCAGCAGA TAATGAGCTT CAATCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA 
CACGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATCA ATCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGAAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATATT GAACAAACCA GTTGGACTGA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA ATGTGGTTTG 
GAAATGTACT TCTCTGTCGA TAAAGAAATT TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCTAATGGTG GCAATATTCT TGTGACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GGTTGTCTCG AGGTGTTGAA GAACAAACAC AAGCTTTCTT TGAGGGCTTT 
AATGAAATCC TTCCCCAGCA GTATTTGCAA TACTTTGACG CAAAGGAATT AGAGGTCCTT 
TTATGCGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TACACTAGGA CAAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGACCA CAAAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGT TGCCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AGCTGAAGGA AAAGCTTTTA TTTGCCATTG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962480.1
CDS	45..2633
Translation	MSGSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIFETLKSNNMKLEEVVVTLQLVGDKEPTETIGDLSVCLDGLQLESEVVTNGETTCSESVPQNDDGSRPKDETRTNTNGSDEPEDTGTGENRKVNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNINSNTSEGATSGLIIPLTISGGSGPRPLNPVPQAPLPPGWEQRVDQHGRVYYVDHIEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFTTSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFNPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_014107005.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107005.1

ORF Insert Sequence:

ATGTCTGGCA GTGGATCGCA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGC 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATCGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATCTTTG AAACCTTAAA GTCAAACAAC ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCTGT CTGTCTTGAT 
GGGCTGCAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGTT 
CCACAGAATG ATGATGGCTC TAGACCGAAG GATGAAACAA GAACAAACAC AAATGGATCA 
GATGAGCCTG AGGATACTGG GACTGGTGAA AATAGGAAGG TCAATGGAAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCTGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGATCCAGTA CAGGCTCTCT GCCACCAACA AATATAAATT CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTACCTC AGGCTCCCCT ACCACCTGGT TGGGAACAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCGGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ACTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCGACATTG GAATCTGTCC GGAATTATGA ACAGTGGCAG 
CTCCAACGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT TATTTATGGG 
AATCAAGATT TGTTTACTAC ATCACAAAAT AAAGAATTTG ATCCCCTTGG TCCCCTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAGT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACAGTTGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATTGA TCCCCGCACT GGAAAATCTG CACTAGACAA TGGACCACAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAA GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACGTTGTT TGAAGATTCC 
TTTCAGCAGA TAATGAGCTT CAATCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA 
CACGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATCA ATCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGAAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATATT GAACAAACCA GTTGGACTGA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA ATGTGGTTTG 
GAAATGTACT TCTCTGTCGA TAAAGAAATT TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCTAATGGTG GCAATATTCT TGTGACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GGTTGTCTCG AGGTGTTGAA GAACAAACAC AAGCTTTCTT TGAGGGCTTT 
AATGAAATCC TTCCCCAGCA GTATTTGCAA TACTTTGACG CAAAGGAATT AGAGGTCCTT 
TTATGCGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TACACTAGGA CAAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGACCA CAAAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGT TGCCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AGCTGAAGGA AAAGCTTTTA TTTGCCATTG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_014107005.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962480.1 Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe24762
Clone ID Related Accession (Same CDS sequence)	XM_022409130.1 , XM_014107005.1 , XM_014107005.2 , XM_846367.5 , XM_846367.4
Accession Version	XM_014107005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCA GTGGATCGCA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGC 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATCGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATCTTTG AAACCTTAAA GTCAAACAAC ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCTGT CTGTCTTGAT 
GGGCTGCAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGTT 
CCACAGAATG ATGATGGCTC TAGACCGAAG GATGAAACAA GAACAAACAC AAATGGATCA 
GATGAGCCTG AGGATACTGG GACTGGTGAA AATAGGAAGG TCAATGGAAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCTGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGATCCAGTA CAGGCTCTCT GCCACCAACA AATATAAATT CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTACCTC AGGCTCCCCT ACCACCTGGT TGGGAACAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCGGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ACTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCGACATTG GAATCTGTCC GGAATTATGA ACAGTGGCAG 
CTCCAACGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT TATTTATGGG 
AATCAAGATT TGTTTACTAC ATCACAAAAT AAAGAATTTG ATCCCCTTGG TCCCCTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAGT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACAGTTGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATTGA TCCCCGCACT GGAAAATCTG CACTAGACAA TGGACCACAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAA GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACGTTGTT TGAAGATTCC 
TTTCAGCAGA TAATGAGCTT CAATCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA 
CACGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATCA ATCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGAAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATATT GAACAAACCA GTTGGACTGA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA ATGTGGTTTG 
GAAATGTACT TCTCTGTCGA TAAAGAAATT TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCTAATGGTG GCAATATTCT TGTGACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GGTTGTCTCG AGGTGTTGAA GAACAAACAC AAGCTTTCTT TGAGGGCTTT 
AATGAAATCC TTCCCCAGCA GTATTTGCAA TACTTTGACG CAAAGGAATT AGAGGTCCTT 
TTATGCGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TACACTAGGA CAAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGACCA CAAAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGT TGCCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AGCTGAAGGA AAAGCTTTTA TTTGCCATTG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962480.1
CDS	47..2635
Translation	MSGSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIFETLKSNNMKLEEVVVTLQLVGDKEPTETIGDLSVCLDGLQLESEVVTNGETTCSESVPQNDDGSRPKDETRTNTNGSDEPEDTGTGENRKVNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNINSNTSEGATSGLIIPLTISGGSGPRPLNPVPQAPLPPGWEQRVDQHGRVYYVDHIEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFTTSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFNPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_014107005.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107005.2

ORF Insert Sequence:

ATGTCTGGCA GTGGATCGCA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGC 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATCGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATCTTTG AAACCTTAAA GTCAAACAAC ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCTGT CTGTCTTGAT 
GGGCTGCAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGTT 
CCACAGAATG ATGATGGCTC TAGACCGAAG GATGAAACAA GAACAAACAC AAATGGATCA 
GATGAGCCTG AGGATACTGG GACTGGTGAA AATAGGAAGG TCAATGGAAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCTGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGATCCAGTA CAGGCTCTCT GCCACCAACA AATATAAATT CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTACCTC AGGCTCCCCT ACCACCTGGT TGGGAACAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCGGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ACTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCGACATTG GAATCTGTCC GGAATTATGA ACAGTGGCAG 
CTCCAACGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT TATTTATGGG 
AATCAAGATT TGTTTACTAC ATCACAAAAT AAAGAATTTG ATCCCCTTGG TCCCCTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAGT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACAGTTGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATTGA TCCCCGCACT GGAAAATCTG CACTAGACAA TGGACCACAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAA GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACGTTGTT TGAAGATTCC 
TTTCAGCAGA TAATGAGCTT CAATCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA 
CACGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATCA ATCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGAAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATATT GAACAAACCA GTTGGACTGA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA ATGTGGTTTG 
GAAATGTACT TCTCTGTCGA TAAAGAAATT TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCTAATGGTG GCAATATTCT TGTGACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GGTTGTCTCG AGGTGTTGAA GAACAAACAC AAGCTTTCTT TGAGGGCTTT 
AATGAAATCC TTCCCCAGCA GTATTTGCAA TACTTTGACG CAAAGGAATT AGAGGTCCTT 
TTATGCGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TACACTAGGA CAAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGACCA CAAAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGT TGCCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AGCTGAAGGA AAAGCTTTTA TTTGCCATTG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_846367.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_851460.1 Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe24762
Clone ID Related Accession (Same CDS sequence)	XM_022409130.1 , XM_014107005.1 , XM_014107005.2 , XM_846367.5 , XM_846367.4
Accession Version	XM_846367.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_846367.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCA GTGGATCGCA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGC 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATCGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATCTTTG AAACCTTAAA GTCAAACAAC ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCTGT CTGTCTTGAT 
GGGCTGCAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGTT 
CCACAGAATG ATGATGGCTC TAGACCGAAG GATGAAACAA GAACAAACAC AAATGGATCA 
GATGAGCCTG AGGATACTGG GACTGGTGAA AATAGGAAGG TCAATGGAAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCTGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGATCCAGTA CAGGCTCTCT GCCACCAACA AATATAAATT CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTACCTC AGGCTCCCCT ACCACCTGGT TGGGAACAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCGGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ACTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCGACATTG GAATCTGTCC GGAATTATGA ACAGTGGCAG 
CTCCAACGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT TATTTATGGG 
AATCAAGATT TGTTTACTAC ATCACAAAAT AAAGAATTTG ATCCCCTTGG TCCCCTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAGT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACAGTTGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATTGA TCCCCGCACT GGAAAATCTG CACTAGACAA TGGACCACAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAA GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACGTTGTT TGAAGATTCC 
TTTCAGCAGA TAATGAGCTT CAATCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA 
CACGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATCA ATCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGAAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATATT GAACAAACCA GTTGGACTGA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA ATGTGGTTTG 
GAAATGTACT TCTCTGTCGA TAAAGAAATT TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCTAATGGTG GCAATATTCT TGTGACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GGTTGTCTCG AGGTGTTGAA GAACAAACAC AAGCTTTCTT TGAGGGCTTT 
AATGAAATCC TTCCCCAGCA GTATTTGCAA TACTTTGACG CAAAGGAATT AGAGGTCCTT 
TTATGCGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TACACTAGGA CAAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGACCA CAAAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGT TGCCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AGCTGAAGGA AAAGCTTTTA TTTGCCATTG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_851460.1
CDS	241..2829
Translation	MSGSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIFETLKSNNMKLEEVVVTLQLVGDKEPTETIGDLSVCLDGLQLESEVVTNGETTCSESVPQNDDGSRPKDETRTNTNGSDEPEDTGTGENRKVNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNINSNTSEGATSGLIIPLTISGGSGPRPLNPVPQAPLPPGWEQRVDQHGRVYYVDHIEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFTTSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFNPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_846367.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_846367.5

ORF Insert Sequence:

ATGTCTGGCA GTGGATCGCA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGC 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATCGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATCTTTG AAACCTTAAA GTCAAACAAC ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCTGT CTGTCTTGAT 
GGGCTGCAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGTT 
CCACAGAATG ATGATGGCTC TAGACCGAAG GATGAAACAA GAACAAACAC AAATGGATCA 
GATGAGCCTG AGGATACTGG GACTGGTGAA AATAGGAAGG TCAATGGAAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCTGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGATCCAGTA CAGGCTCTCT GCCACCAACA AATATAAATT CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTACCTC AGGCTCCCCT ACCACCTGGT TGGGAACAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCGGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ACTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCGACATTG GAATCTGTCC GGAATTATGA ACAGTGGCAG 
CTCCAACGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT TATTTATGGG 
AATCAAGATT TGTTTACTAC ATCACAAAAT AAAGAATTTG ATCCCCTTGG TCCCCTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAGT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACAGTTGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATTGA TCCCCGCACT GGAAAATCTG CACTAGACAA TGGACCACAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAA GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACGTTGTT TGAAGATTCC 
TTTCAGCAGA TAATGAGCTT CAATCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA 
CACGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATCA ATCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGAAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATATT GAACAAACCA GTTGGACTGA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA ATGTGGTTTG 
GAAATGTACT TCTCTGTCGA TAAAGAAATT TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCTAATGGTG GCAATATTCT TGTGACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GGTTGTCTCG AGGTGTTGAA GAACAAACAC AAGCTTTCTT TGAGGGCTTT 
AATGAAATCC TTCCCCAGCA GTATTTGCAA TACTTTGACG CAAAGGAATT AGAGGTCCTT 
TTATGCGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TACACTAGGA CAAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGACCA CAAAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGT TGCCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AGCTGAAGGA AAAGCTTTTA TTTGCCATTG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_846367.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_851460.1 Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe24762
Clone ID Related Accession (Same CDS sequence)	XM_022409130.1 , XM_014107005.1 , XM_014107005.2 , XM_846367.5 , XM_846367.4
Accession Version	XM_846367.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_846367.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCA GTGGATCGCA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGC 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATCGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATCTTTG AAACCTTAAA GTCAAACAAC ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCTGT CTGTCTTGAT 
GGGCTGCAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGTT 
CCACAGAATG ATGATGGCTC TAGACCGAAG GATGAAACAA GAACAAACAC AAATGGATCA 
GATGAGCCTG AGGATACTGG GACTGGTGAA AATAGGAAGG TCAATGGAAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCTGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGATCCAGTA CAGGCTCTCT GCCACCAACA AATATAAATT CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTACCTC AGGCTCCCCT ACCACCTGGT TGGGAACAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCGGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ACTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCGACATTG GAATCTGTCC GGAATTATGA ACAGTGGCAG 
CTCCAACGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT TATTTATGGG 
AATCAAGATT TGTTTACTAC ATCACAAAAT AAAGAATTTG ATCCCCTTGG TCCCCTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAGT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACAGTTGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATTGA TCCCCGCACT GGAAAATCTG CACTAGACAA TGGACCACAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAA GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACGTTGTT TGAAGATTCC 
TTTCAGCAGA TAATGAGCTT CAATCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA 
CACGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATCA ATCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGAAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATATT GAACAAACCA GTTGGACTGA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA ATGTGGTTTG 
GAAATGTACT TCTCTGTCGA TAAAGAAATT TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCTAATGGTG GCAATATTCT TGTGACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GGTTGTCTCG AGGTGTTGAA GAACAAACAC AAGCTTTCTT TGAGGGCTTT 
AATGAAATCC TTCCCCAGCA GTATTTGCAA TACTTTGACG CAAAGGAATT AGAGGTCCTT 
TTATGCGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TACACTAGGA CAAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGACCA CAAAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGT TGCCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AGCTGAAGGA AAAGCTTTTA TTTGCCATTG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_851460.1
CDS	199..2787
Translation	MSGSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIFETLKSNNMKLEEVVVTLQLVGDKEPTETIGDLSVCLDGLQLESEVVTNGETTCSESVPQNDDGSRPKDETRTNTNGSDEPEDTGTGENRKVNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNINSNTSEGATSGLIIPLTISGGSGPRPLNPVPQAPLPPGWEQRVDQHGRVYYVDHIEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFTTSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFNPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_846367.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_846367.4

ORF Insert Sequence:

ATGTCTGGCA GTGGATCGCA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGC 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATCGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATCTTTG AAACCTTAAA GTCAAACAAC ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCTGT CTGTCTTGAT 
GGGCTGCAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGTT 
CCACAGAATG ATGATGGCTC TAGACCGAAG GATGAAACAA GAACAAACAC AAATGGATCA 
GATGAGCCTG AGGATACTGG GACTGGTGAA AATAGGAAGG TCAATGGAAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCTGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGATCCAGTA CAGGCTCTCT GCCACCAACA AATATAAATT CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTACCTC AGGCTCCCCT ACCACCTGGT TGGGAACAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCGGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ACTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCGACATTG GAATCTGTCC GGAATTATGA ACAGTGGCAG 
CTCCAACGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT TATTTATGGG 
AATCAAGATT TGTTTACTAC ATCACAAAAT AAAGAATTTG ATCCCCTTGG TCCCCTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAGT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACAGTTGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATTGA TCCCCGCACT GGAAAATCTG CACTAGACAA TGGACCACAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAA GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACGTTGTT TGAAGATTCC 
TTTCAGCAGA TAATGAGCTT CAATCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA 
CACGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATCA ATCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGAAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATATT GAACAAACCA GTTGGACTGA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA ATGTGGTTTG 
GAAATGTACT TCTCTGTCGA TAAAGAAATT TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCTAATGGTG GCAATATTCT TGTGACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GGTTGTCTCG AGGTGTTGAA GAACAAACAC AAGCTTTCTT TGAGGGCTTT 
AATGAAATCC TTCCCCAGCA GTATTTGCAA TACTTTGACG CAAAGGAATT AGAGGTCCTT 
TTATGCGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TACACTAGGA CAAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGACCA CAAAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGT TGCCCAGAAG TCATACCTGT TTTAATCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AGCTGAAGGA AAAGCTTTTA TTTGCCATTG AAGAAACAGA AGGATTTGGA 
CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_005634950.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005635007.1 Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe24763
Clone ID Related Accession (Same CDS sequence)	XM_005634950.3 , XM_005634950.2
Accession Version	XM_005634950.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634950.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCA GTGGATCGCA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGC 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATCGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATCTTTG AAACCTTAAA GTCAAACAAC ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCTGT CTGTCTTGAT 
GGGCTGCAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGTT 
CCACAGAATG ATGATGGCTC TAGACCGAAG GATGAAACAA GAACAAACAC AAATGGATCA 
GATGAGCCTG AGGATACTGG GACTGGTGAA AATAGGAAGG TCAATGGAAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCTGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGATCCAGTA CAGGCTCTCT GCCACCAACA AATATAAATT CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTACCTC AGGCTCCCCT ACCACCTGGT TGGGAACAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCGGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ACTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCGACATTG GAATCTGTCC GGAATTATGA ACAGTGGCAG 
CTCCAACGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT TATTTATGGG 
AATCAAGATT TGTTTACTAC ATCACAAAAT AAAGAATTTG ATCCCCTTGG TCCCCTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAGT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACAGTTGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATTGA TCCCCGCACT GGAAAATCTG CACTAGACAA TGGACCACAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAA GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACGTTGTT TGAAGATTCC 
TTTCAGCAGA TAATGAGCTT CAATCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA 
CACGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATCA ATCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGAAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATATT GAACAAACCA GTTGGACTGA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA ATGTGGTTTG 
GAAATGTACT TCTCTGTCGA TAAAGAAATT TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCTAATGGTG GCAATATTCT TGTGACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GGTTGTCTCG AGGTGTTGAA GAACAAACAC AAGCTTTCTT TGAGGGCTTT 
AATGAAATCC TTCCCCAGCA GTATTTGCAA TACTTTGACG CAAAGGAATT AGAGGTCCTT 
TTATGCGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TACACTAGGA CAAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGACCA CAAAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGT TGCCCAGAAG TCATACCTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635007.1
CDS	241..2733
Translation	MSGSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIFETLKSNNMKLEEVVVTLQLVGDKEPTETIGDLSVCLDGLQLESEVVTNGETTCSESVPQNDDGSRPKDETRTNTNGSDEPEDTGTGENRKVNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNINSNTSEGATSGLIIPLTISGGSGPRPLNPVPQAPLPPGWEQRVDQHGRVYYVDHIEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFTTSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFNPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTC

Target ORF information:

RefSeq Version	XM_005634950.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634950.3

ORF Insert Sequence:

ATGTCTGGCA GTGGATCGCA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGC 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATCGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATCTTTG AAACCTTAAA GTCAAACAAC ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCTGT CTGTCTTGAT 
GGGCTGCAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGTT 
CCACAGAATG ATGATGGCTC TAGACCGAAG GATGAAACAA GAACAAACAC AAATGGATCA 
GATGAGCCTG AGGATACTGG GACTGGTGAA AATAGGAAGG TCAATGGAAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCTGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGATCCAGTA CAGGCTCTCT GCCACCAACA AATATAAATT CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTACCTC AGGCTCCCCT ACCACCTGGT TGGGAACAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCGGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ACTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCGACATTG GAATCTGTCC GGAATTATGA ACAGTGGCAG 
CTCCAACGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT TATTTATGGG 
AATCAAGATT TGTTTACTAC ATCACAAAAT AAAGAATTTG ATCCCCTTGG TCCCCTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAGT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACAGTTGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATTGA TCCCCGCACT GGAAAATCTG CACTAGACAA TGGACCACAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAA GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACGTTGTT TGAAGATTCC 
TTTCAGCAGA TAATGAGCTT CAATCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA 
CACGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATCA ATCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGAAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATATT GAACAAACCA GTTGGACTGA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA ATGTGGTTTG 
GAAATGTACT TCTCTGTCGA TAAAGAAATT TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCTAATGGTG GCAATATTCT TGTGACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GGTTGTCTCG AGGTGTTGAA GAACAAACAC AAGCTTTCTT TGAGGGCTTT 
AATGAAATCC TTCCCCAGCA GTATTTGCAA TACTTTGACG CAAAGGAATT AGAGGTCCTT 
TTATGCGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TACACTAGGA CAAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGACCA CAAAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGT TGCCCAGAAG TCATACCTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_005634950.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005635007.1 Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe24763
Clone ID Related Accession (Same CDS sequence)	XM_005634950.3 , XM_005634950.2
Accession Version	XM_005634950.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005634950.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCA GTGGATCGCA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGC 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATCGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATCTTTG AAACCTTAAA GTCAAACAAC ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCTGT CTGTCTTGAT 
GGGCTGCAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGTT 
CCACAGAATG ATGATGGCTC TAGACCGAAG GATGAAACAA GAACAAACAC AAATGGATCA 
GATGAGCCTG AGGATACTGG GACTGGTGAA AATAGGAAGG TCAATGGAAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCTGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGATCCAGTA CAGGCTCTCT GCCACCAACA AATATAAATT CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTACCTC AGGCTCCCCT ACCACCTGGT TGGGAACAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCGGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ACTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCGACATTG GAATCTGTCC GGAATTATGA ACAGTGGCAG 
CTCCAACGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT TATTTATGGG 
AATCAAGATT TGTTTACTAC ATCACAAAAT AAAGAATTTG ATCCCCTTGG TCCCCTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAGT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACAGTTGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATTGA TCCCCGCACT GGAAAATCTG CACTAGACAA TGGACCACAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAA GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACGTTGTT TGAAGATTCC 
TTTCAGCAGA TAATGAGCTT CAATCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA 
CACGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATCA ATCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGAAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATATT GAACAAACCA GTTGGACTGA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA ATGTGGTTTG 
GAAATGTACT TCTCTGTCGA TAAAGAAATT TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCTAATGGTG GCAATATTCT TGTGACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GGTTGTCTCG AGGTGTTGAA GAACAAACAC AAGCTTTCTT TGAGGGCTTT 
AATGAAATCC TTCCCCAGCA GTATTTGCAA TACTTTGACG CAAAGGAATT AGAGGTCCTT 
TTATGCGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TACACTAGGA CAAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGACCA CAAAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGT TGCCCAGAAG TCATACCTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635007.1
CDS	199..2691
Translation	MSGSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIFETLKSNNMKLEEVVVTLQLVGDKEPTETIGDLSVCLDGLQLESEVVTNGETTCSESVPQNDDGSRPKDETRTNTNGSDEPEDTGTGENRKVNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNINSNTSEGATSGLIIPLTISGGSGPRPLNPVPQAPLPPGWEQRVDQHGRVYYVDHIEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFTTSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFNPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTC

Target ORF information:

RefSeq Version	XM_005634950.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634950.2

ORF Insert Sequence:

ATGTCTGGCA GTGGATCGCA GCTTGGTTCA ATGGGTAGCC TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGC 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA ACCCCTTACA GTTATCGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGCATTA 
GATATCTTTG AAACCTTAAA GTCAAACAAC ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATAGGAG ACTTGTCTGT CTGTCTTGAT 
GGGCTGCAGT TAGAGTCTGA AGTTGTTACC AATGGTGAAA CTACATGTTC AGAAAGTGTT 
CCACAGAATG ATGATGGCTC TAGACCGAAG GATGAAACAA GAACAAACAC AAATGGATCA 
GATGAGCCTG AGGATACTGG GACTGGTGAA AATAGGAAGG TCAATGGAAA TAATTCTCCA 
TCACTCTCAA ATGGTGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCTGC ATCTGTCAAT GGTTCACCAT CTGCCACTTC TGAAAGTGAT 
GGATCCAGTA CAGGCTCTCT GCCACCAACA AATATAAATT CAAATACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGCT CAGGCCCTAG GCCATTAAAT 
CCTGTACCTC AGGCTCCCCT ACCACCTGGT TGGGAACAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCGGA ACCTCTACCT 
CCTGGCTGGG AACGGCGGGT TGACAACATG GGACGTATTT ACTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCGACATTG GAATCTGTCC GGAATTATGA ACAGTGGCAG 
CTCCAACGTA GTCAGCTTCA AGGAGCAATG CAGCAGTTTA ACCAGAGATT TATTTATGGG 
AATCAAGATT TGTTTACTAC ATCACAAAAT AAAGAATTTG ATCCCCTTGG TCCCCTGCCA 
CCTGGATGGG AGAAGAGAAC AGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAGT CAAGGTCAGT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACAGTTGAT GGAATTCCAT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATTGA TCCCCGCACT GGAAAATCTG CACTAGACAA TGGACCACAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAA GTTCAGTATT TCCGGTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACGTTGTT TGAAGATTCC 
TTTCAGCAGA TAATGAGCTT CAATCCCCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA 
CACGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATCA ATCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGAAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATATT GAACAAACCA GTTGGACTGA AGGATTTAGA ATCTATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA ATGTGGTTTG 
GAAATGTACT TCTCTGTCGA TAAAGAAATT TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCTAATGGTG GCAATATTCT TGTGACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GGTTGTCTCG AGGTGTTGAA GAACAAACAC AAGCTTTCTT TGAGGGCTTT 
AATGAAATCC TTCCCCAGCA GTATTTGCAA TACTTTGACG CAAAGGAATT AGAGGTCCTT 
TTATGCGGAA TGCAAGAGAT TGATTTGAAT GACTGGCAAA GACATGCCAT CTACCGTCAT 
TACACTAGGA CAAGCAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATTGATAAT 
GAGAAGAGAA TGAGACTTCT GCAGTTTGTT ACTGGAACCT GCCGATTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGACCA CAAAAATTCT GCATTGAAAA AGTTGGGAAA 
GAAAATTGGT TGCCCAGAAG TCATACCTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_022409131.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022264839.1 Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe296788
Clone ID Related Accession (Same CDS sequence)	XM_022409131.1
Accession Version	XM_022409131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTTG AGGAAGTAGT TGTGACTTTA CAGCTTGTAG GTGACAAAGA GCCAACAGAG 
ACAATAGGAG ACTTGTCTGT CTGTCTTGAT GGGCTGCAGT TAGAGTCTGA AGTTGTTACC 
AATGGTGAAA CTACATGTTC AGAAAGTGTT CCACAGAATG ATGATGGCTC TAGACCGAAG 
GATGAAACAA GAACAAACAC AAATGGATCA GATGAGCCTG AGGATACTGG GACTGGTGAA 
AATAGGAAGG TCAATGGAAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGGCCTCCAA GACCTTCACG ACCACCTCCA CCTACCCCAC GTAGACCTGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGATCCAGTA CAGGCTCTCT GCCACCAACA 
AATATAAATT CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTACCTC AGGCTCCCCT ACCACCTGGT 
TGGGAACAGA GAGTGGACCA GCATGGGCGA GTTTACTATG TAGATCATAT TGAAAAAAGA 
ACAACGTGGG ATAGACCGGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ACTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCGACATTG 
GAATCTGTCC GGAATTATGA ACAGTGGCAG CTCCAACGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT TATTTATGGG AATCAAGATT TGTTTACTAC ATCACAAAAT 
AAAGAATTTG ATCCCCTTGG TCCCCTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGTAGAGTAT ATTTTGTCAA CCACAACACT CGTATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAGT TAAATGAAAA GCCCTTACCT GAAGGCTGGG AAATGAGATT CACAGTTGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACAACCA CCTATATTGA TCCCCGCACT 
GGAAAATCTG CACTAGACAA TGGACCACAG ATAGCCTATG TACGGGACTT CAAGGCAAAA 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACGTTGTT TGAAGATTCC TTTCAGCAGA TAATGAGCTT CAATCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA CACGAGGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATCA ATCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGGTTTA TTGCTATGGC TCTGTTCCAT 
GGAAAATTCA TAGACACAGG TTTTTCTTTG CCATTCTATA AGCGTATATT GAACAAACCA 
GTTGGACTGA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAAGAAAACA ATATTGAGGA ATGTGGTTTG GAAATGTACT TCTCTGTCGA TAAAGAAATT 
TTAGGTGAAA TCAAGAGTCA TGATTTGAAA CCTAATGGTG GCAATATTCT TGTGACAGAA 
GAAAATAAAG AGGAATATAT CAGAATGGTA GCTGAATGGA GGTTGTCTCG AGGTGTTGAA 
GAACAAACAC AAGCTTTCTT TGAGGGCTTT AATGAAATCC TTCCCCAGCA GTATTTGCAA 
TACTTTGACG CAAAGGAATT AGAGGTCCTT TTATGCGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TACACTAGGA CAAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTTGGAGGA TTTGCTGACC TCATGGGGAG CAATGGACCA 
CAAAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGT TGCCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCCTACAAG AGCTATGAGC AGCTGAAGGA AAAGCTTTTA 
TTTGCCATTG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264839.1
CDS	210..2468
Translation	MKLEEVVVTLQLVGDKEPTETIGDLSVCLDGLQLESEVVTNGETTCSESVPQNDDGSRPKDETRTNTNGSDEPEDTGTGENRKVNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNINSNTSEGATSGLIIPLTISGGSGPRPLNPVPQAPLPPGWEQRVDQHGRVYYVDHIEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFTTSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFNPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_022409131.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409131.1

ORF Insert Sequence:

ATGAAACTTG AGGAAGTAGT TGTGACTTTA CAGCTTGTAG GTGACAAAGA GCCAACAGAG 
ACAATAGGAG ACTTGTCTGT CTGTCTTGAT GGGCTGCAGT TAGAGTCTGA AGTTGTTACC 
AATGGTGAAA CTACATGTTC AGAAAGTGTT CCACAGAATG ATGATGGCTC TAGACCGAAG 
GATGAAACAA GAACAAACAC AAATGGATCA GATGAGCCTG AGGATACTGG GACTGGTGAA 
AATAGGAAGG TCAATGGAAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGGCCTCCAA GACCTTCACG ACCACCTCCA CCTACCCCAC GTAGACCTGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGATCCAGTA CAGGCTCTCT GCCACCAACA 
AATATAAATT CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTACCTC AGGCTCCCCT ACCACCTGGT 
TGGGAACAGA GAGTGGACCA GCATGGGCGA GTTTACTATG TAGATCATAT TGAAAAAAGA 
ACAACGTGGG ATAGACCGGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ACTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCGACATTG 
GAATCTGTCC GGAATTATGA ACAGTGGCAG CTCCAACGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT TATTTATGGG AATCAAGATT TGTTTACTAC ATCACAAAAT 
AAAGAATTTG ATCCCCTTGG TCCCCTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGTAGAGTAT ATTTTGTCAA CCACAACACT CGTATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAGT TAAATGAAAA GCCCTTACCT GAAGGCTGGG AAATGAGATT CACAGTTGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACAACCA CCTATATTGA TCCCCGCACT 
GGAAAATCTG CACTAGACAA TGGACCACAG ATAGCCTATG TACGGGACTT CAAGGCAAAA 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACGTTGTT TGAAGATTCC TTTCAGCAGA TAATGAGCTT CAATCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA CACGAGGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATCA ATCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGGTTTA TTGCTATGGC TCTGTTCCAT 
GGAAAATTCA TAGACACAGG TTTTTCTTTG CCATTCTATA AGCGTATATT GAACAAACCA 
GTTGGACTGA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAAGAAAACA ATATTGAGGA ATGTGGTTTG GAAATGTACT TCTCTGTCGA TAAAGAAATT 
TTAGGTGAAA TCAAGAGTCA TGATTTGAAA CCTAATGGTG GCAATATTCT TGTGACAGAA 
GAAAATAAAG AGGAATATAT CAGAATGGTA GCTGAATGGA GGTTGTCTCG AGGTGTTGAA 
GAACAAACAC AAGCTTTCTT TGAGGGCTTT AATGAAATCC TTCCCCAGCA GTATTTGCAA 
TACTTTGACG CAAAGGAATT AGAGGTCCTT TTATGCGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TACACTAGGA CAAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTTGGAGGA TTTGCTGACC TCATGGGGAG CAATGGACCA 
CAAAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGT TGCCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCCTACAAG AGCTATGAGC AGCTGAAGGA AAAGCTTTTA 
TTTGCCATTG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_022409132.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022264840.1 Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe296789
Clone ID Related Accession (Same CDS sequence)	XM_022409132.1
Accession Version	XM_022409132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA AAACAAACAC AAATGGATCA GATGAGCCTG AGGATACTGG GACTGGTGAA 
AATAGGAAGG TCAATGGAAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGGCCTCCAA GACCTTCACG ACCACCTCCA CCTACCCCAC GTAGACCTGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGATCCAGTA CAGGCTCTCT GCCACCAACA 
AATATAAATT CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTACCTC AGGCTCCCCT ACCACCTGGT 
TGGGAACAGA GAGTGGACCA GCATGGGCGA GTTTACTATG TAGATCATAT TGAAAAAAGA 
ACAACGTGGG ATAGACCGGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ACTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCGACATTG 
GAATCTGTCC GGAATTATGA ACAGTGGCAG CTCCAACGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT TATTTATGGG AATCAAGATT TGTTTACTAC ATCACAAAAT 
AAAGAATTTG ATCCCCTTGG TCCCCTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGTAGAGTAT ATTTTGTCAA CCACAACACT CGTATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAGT TAAATGAAAA GCCCTTACCT GAAGGCTGGG AAATGAGATT CACAGTTGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACAACCA CCTATATTGA TCCCCGCACT 
GGAAAATCTG CACTAGACAA TGGACCACAG ATAGCCTATG TACGGGACTT CAAGGCAAAA 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACGTTGTT TGAAGATTCC TTTCAGCAGA TAATGAGCTT CAATCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA CACGAGGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATCA ATCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGGTTTA TTGCTATGGC TCTGTTCCAT 
GGAAAATTCA TAGACACAGG TTTTTCTTTG CCATTCTATA AGCGTATATT GAACAAACCA 
GTTGGACTGA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAAGAAAACA ATATTGAGGA ATGTGGTTTG GAAATGTACT TCTCTGTCGA TAAAGAAATT 
TTAGGTGAAA TCAAGAGTCA TGATTTGAAA CCTAATGGTG GCAATATTCT TGTGACAGAA 
GAAAATAAAG AGGAATATAT CAGAATGGTA GCTGAATGGA GGTTGTCTCG AGGTGTTGAA 
GAACAAACAC AAGCTTTCTT TGAGGGCTTT AATGAAATCC TTCCCCAGCA GTATTTGCAA 
TACTTTGACG CAAAGGAATT AGAGGTCCTT TTATGCGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TACACTAGGA CAAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTTGGAGGA TTTGCTGACC TCATGGGGAG CAATGGACCA 
CAAAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGT TGCCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCCTACAAG AGCTATGAGC AGCTGAAGGA AAAGCTTTTA 
TTTGCCATTG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264840.1
CDS	128..2206
Translation	MFRKTNTNGSDEPEDTGTGENRKVNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNINSNTSEGATSGLIIPLTISGGSGPRPLNPVPQAPLPPGWEQRVDQHGRVYYVDHIEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFTTSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFNPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_022409132.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris itchy E3 ubiquitin protein ligase (ITCH), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409132.1

ORF Insert Sequence:

ATGTTCAGAA AAACAAACAC AAATGGATCA GATGAGCCTG AGGATACTGG GACTGGTGAA 
AATAGGAAGG TCAATGGAAA TAATTCTCCA TCACTCTCAA ATGGTGGTTT TAAACCTTCT 
AGGCCTCCAA GACCTTCACG ACCACCTCCA CCTACCCCAC GTAGACCTGC ATCTGTCAAT 
GGTTCACCAT CTGCCACTTC TGAAAGTGAT GGATCCAGTA CAGGCTCTCT GCCACCAACA 
AATATAAATT CAAATACATC TGAAGGAGCA ACATCTGGAT TAATAATTCC TCTTACTATA 
TCTGGAGGCT CAGGCCCTAG GCCATTAAAT CCTGTACCTC AGGCTCCCCT ACCACCTGGT 
TGGGAACAGA GAGTGGACCA GCATGGGCGA GTTTACTATG TAGATCATAT TGAAAAAAGA 
ACAACGTGGG ATAGACCGGA ACCTCTACCT CCTGGCTGGG AACGGCGGGT TGACAACATG 
GGACGTATTT ACTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCGACATTG 
GAATCTGTCC GGAATTATGA ACAGTGGCAG CTCCAACGTA GTCAGCTTCA AGGAGCAATG 
CAGCAGTTTA ACCAGAGATT TATTTATGGG AATCAAGATT TGTTTACTAC ATCACAAAAT 
AAAGAATTTG ATCCCCTTGG TCCCCTGCCA CCTGGATGGG AGAAGAGAAC AGACAGCAAT 
GGTAGAGTAT ATTTTGTCAA CCACAACACT CGTATTACAC AATGGGAAGA CCCCAGAAGT 
CAAGGTCAGT TAAATGAAAA GCCCTTACCT GAAGGCTGGG AAATGAGATT CACAGTTGAT 
GGAATTCCAT ATTTTGTGGA CCACAATAGA AGAACAACCA CCTATATTGA TCCCCGCACT 
GGAAAATCTG CACTAGACAA TGGACCACAG ATAGCCTATG TACGGGACTT CAAGGCAAAA 
GTTCAGTATT TCCGGTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACGTTGTT TGAAGATTCC TTTCAGCAGA TAATGAGCTT CAATCCCCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AGTGGTTCTT TCTTTTGTCA CACGAGGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATCA ATCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGGTTTA TTGCTATGGC TCTGTTCCAT 
GGAAAATTCA TAGACACAGG TTTTTCTTTG CCATTCTATA AGCGTATATT GAACAAACCA 
GTTGGACTGA AGGATTTAGA ATCTATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAAGAAAACA ATATTGAGGA ATGTGGTTTG GAAATGTACT TCTCTGTCGA TAAAGAAATT 
TTAGGTGAAA TCAAGAGTCA TGATTTGAAA CCTAATGGTG GCAATATTCT TGTGACAGAA 
GAAAATAAAG AGGAATATAT CAGAATGGTA GCTGAATGGA GGTTGTCTCG AGGTGTTGAA 
GAACAAACAC AAGCTTTCTT TGAGGGCTTT AATGAAATCC TTCCCCAGCA GTATTTGCAA 
TACTTTGACG CAAAGGAATT AGAGGTCCTT TTATGCGGAA TGCAAGAGAT TGATTTGAAT 
GACTGGCAAA GACATGCCAT CTACCGTCAT TACACTAGGA CAAGCAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATTGATAAT GAGAAGAGAA TGAGACTTCT GCAGTTTGTT 
ACTGGAACCT GCCGATTGCC AGTTGGAGGA TTTGCTGACC TCATGGGGAG CAATGGACCA 
CAAAAATTCT GCATTGAAAA AGTTGGGAAA GAAAATTGGT TGCCCAGAAG TCATACCTGT 
TTTAATCGCC TGGACCTGCC ACCCTACAAG AGCTATGAGC AGCTGAAGGA AAAGCTTTTA 
TTTGCCATTG AAGAAACAGA AGGATTTGGA CAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ITCH
Entrez Gene ID	477199
Full Name	itchy E3 ubiquitin protein ligase
General protein information	Preferred Names itchy E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase Itchy homolog itchy E3 ubiquitin protein ligase homolog itchy homolog E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

Homo sapiens (human)	ITCH	NP_113671.3
Canis lupus familiaris (dog)	ITCH	XP_851460.1
Mus musculus (house mouse)	Itch	NP_001230641.1
Rattus norvegicus (Norway rat)	Itch	NP_001005887.1
Bos taurus (cattle)	ITCH	NP_001075897.1
Gallus gallus (chicken)	ITCH	XP_417330.2
Danio rerio (zebrafish)	itch	XP_002667578.2
Danio rerio (zebrafish)	LOC100330031	XP_003201702.2
	LOC707640	XP_002808005.1
Xenopus tropicalis (tropical clawed frog)	itch	NP_001072737.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ITCH cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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