PLCB4 cDNA ORF clone, Canis lupus familiaris(dog)

The following PLCB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe42764 XM_022409338.1
Latest version!
Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe42764 XM_014107043.2
Latest version!
Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe42764 XM_022409339.1
Latest version!
Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe42764 XM_022409337.1
Latest version!
Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe42764 XM_014107042.2
Latest version!
Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe42764 XM_022409336.1
Latest version!
Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe42764 XM_022409335.1
Latest version!
Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe24550 XM_014107044.2
Latest version!
Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OCe24550 XM_014107044.1 Canis lupus familiaris phospholipase C, beta 4 (PLCB4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe42764 XM_014107042.1 Canis lupus familiaris phospholipase C, beta 4 (PLCB4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe42764 XM_014107043.1 Canis lupus familiaris phospholipase C, beta 4 (PLCB4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe42764
    Clone ID Related Accession (Same CDS sequence) XM_022409336.1 , XM_022409337.1 , XM_022409339.1 , XM_014107042.1 , XM_014107043.1 , XM_022409335.1 , XM_022409338.1 , XM_014107042.2 , XM_014107043.2
    Accession Version XM_022409336.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265044.1
    CDS201..3821
    Translation

    Target ORF information:

    RefSeq Version XM_022409336.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409336.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42764
    Clone ID Related Accession (Same CDS sequence) XM_022409336.1 , XM_022409337.1 , XM_022409339.1 , XM_014107042.1 , XM_014107043.1 , XM_022409335.1 , XM_022409338.1 , XM_014107042.2 , XM_014107043.2
    Accession Version XM_022409337.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265045.1
    CDS376..3996
    Translation

    Target ORF information:

    RefSeq Version XM_022409337.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409337.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42764
    Clone ID Related Accession (Same CDS sequence) XM_022409336.1 , XM_022409337.1 , XM_022409339.1 , XM_014107042.1 , XM_014107043.1 , XM_022409335.1 , XM_022409338.1 , XM_014107042.2 , XM_014107043.2
    Accession Version XM_022409339.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265047.1
    CDS88..3708
    Translation

    Target ORF information:

    RefSeq Version XM_022409339.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409339.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42764
    Clone ID Related Accession (Same CDS sequence) XM_022409336.1 , XM_022409337.1 , XM_022409339.1 , XM_014107042.1 , XM_014107043.1 , XM_022409335.1 , XM_022409338.1 , XM_014107042.2 , XM_014107043.2
    Accession Version XM_014107042.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013962517.1
    CDS205..3825
    Translation

    Target ORF information:

    RefSeq Version XM_014107042.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phospholipase C, beta 4 (PLCB4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107042.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42764
    Clone ID Related Accession (Same CDS sequence) XM_022409336.1 , XM_022409337.1 , XM_022409339.1 , XM_014107042.1 , XM_014107043.1 , XM_022409335.1 , XM_022409338.1 , XM_014107042.2 , XM_014107043.2
    Accession Version XM_014107043.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013962518.1
    CDS344..3964
    Translation

    Target ORF information:

    RefSeq Version XM_014107043.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phospholipase C, beta 4 (PLCB4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107043.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42764
    Clone ID Related Accession (Same CDS sequence) XM_022409336.1 , XM_022409337.1 , XM_022409339.1 , XM_014107042.1 , XM_014107043.1 , XM_022409335.1 , XM_022409338.1 , XM_014107042.2 , XM_014107043.2
    Accession Version XM_022409335.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265043.1
    CDS237..3857
    Translation

    Target ORF information:

    RefSeq Version XM_022409335.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409335.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42764
    Clone ID Related Accession (Same CDS sequence) XM_022409336.1 , XM_022409337.1 , XM_022409339.1 , XM_014107042.1 , XM_014107043.1 , XM_022409335.1 , XM_022409338.1 , XM_014107042.2 , XM_014107043.2
    Accession Version XM_022409338.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265046.1
    CDS157..3777
    Translation

    Target ORF information:

    RefSeq Version XM_022409338.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409338.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42764
    Clone ID Related Accession (Same CDS sequence) XM_022409336.1 , XM_022409337.1 , XM_022409339.1 , XM_014107042.1 , XM_014107043.1 , XM_022409335.1 , XM_022409338.1 , XM_014107042.2 , XM_014107043.2
    Accession Version XM_014107042.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013962517.1
    CDS205..3825
    Translation

    Target ORF information:

    RefSeq Version XM_014107042.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107042.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42764
    Clone ID Related Accession (Same CDS sequence) XM_022409336.1 , XM_022409337.1 , XM_022409339.1 , XM_014107042.1 , XM_014107043.1 , XM_022409335.1 , XM_022409338.1 , XM_014107042.2 , XM_014107043.2
    Accession Version XM_014107043.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013962518.1
    CDS344..3964
    Translation

    Target ORF information:

    RefSeq Version XM_014107043.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107043.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGCTATT GAAATCCTGC CATGCAGTGT CCCAAACACA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTAGTGTG
    AAACTCCAAA ATGCAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24550
    Clone ID Related Accession (Same CDS sequence) XM_014107044.2 , XM_014107044.1
    Accession Version XM_014107044.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107044.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGGCGAA GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC
    CGCAGACCAG CAACAGTAGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013962519.1
    CDS1..3564
    Translation

    Target ORF information:

    RefSeq Version XM_014107044.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris phospholipase C beta 4 (PLCB4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107044.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGGCGAA GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC
    CGCAGACCAG CAACAGTAGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24550
    Clone ID Related Accession (Same CDS sequence) XM_014107044.2 , XM_014107044.1
    Accession Version XM_014107044.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGGCGAA GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC
    CGCAGACCAG CAACAGTAGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013962519.1
    CDS1..3564
    Translation

    Target ORF information:

    RefSeq Version XM_014107044.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris phospholipase C, beta 4 (PLCB4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107044.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCCATTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTGGATGAAT TTGGCTTCTT TCTGACCTGG AGGAGTGAAG GCAAGGAAGG ACAAGTGCTA
    GAATGCTCCC TCATCAACAG TATTCGATTG GGAGCCACCC CAAAGGATCC CAAAATCTTG
    GCCGCTCTTG AGGCTGTTGG AAAGTCAGAG AATGATCTGG AAGGGCGGAT AGTTTGTGTC
    TGCAGTGGCA CAGATCTGGT GAACATCAGT TTCACATACA TGGTGGCTGA GAATCCAGAA
    GTAACTAAGC AATGGGTAGA AGGATTGAGA TCAATCATAC ATAACTTCAG AGCCAACAAT
    GTTAGTCCAA TGACCTGCCT CAAGAAACAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGTAAAA TTCCAGTCAG GAGTATTACT AGAACGTTTG CGTCGGGGAA AACAGAAAAA
    GTGATCTTTC AAGCACTCAA GGAGTTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCAGCAT TTACTTATGA GAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTTAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAG CGAGACCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCAAAAAG GGCAATGCAG ATCATCGAGA TGTATGAGCC TGATGAAGAT
    TTGAAGAAAA AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATATCTGAT GTCAGATGAA
    AATGCCCCAG TCTTCCTGGA TCGTTTAGAA CTTTACCAAG AAATGGACCA CCCTCTGGCC
    CACTACTTCA TCAGTTCTTC ACATAACACC TATCTCACTG GCCGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTTCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGACG GAAAAGGTGA AGACCAAGAA CCAATAATAA CTCATGGAAA AGCTATGTGT
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCATT TGTCACATCC
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATATT GTGAAGATCT ATTTGGGGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTTGAAC CAGGCAGGCC CTTACCATCC CCCAATGACC TCAAAAGAAA AATACTCATC
    AAAAATAAAC GGCTGAAACC TGAGGTTGAA AAAAAACAGC TCGAGGCTTT GAAAAGCATG
    ATGGAAGCTG GAGAATCTGC TGCCCCAGTT AGCATCCTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGACCAAGA GGAGGAAGCT CACCCTGAAT ACAAATTTGG AAATGAACTT
    TCTGCTGATG ACTTGGGTCA CAAGGAGGCT GTTGCAAATA GTGTCAAGAA GGCGTCGGAT
    GACCTTGAAC ATGAAATCAA CAAAAAGGGC CTGGTTACTG TAGAAGATGA GCAGGCATGG
    ATGGCATCAT ATAAGTATGT AGGTGCTACC ACTAATATCC ATCCATATTT GTCTACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA GCGCAATATT
    CATTATAACA TGTCTTCTTT TAACGAGTCC GTTGGTCTAG GCTACTTGAA GACACATGCA
    ATTGAATTTG TGAATTATAA CAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGG
    GTTGATTCCA GTAATTACAT GCCTCAAATT TTCTGGAATG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAATTGAATC AGGGAAAATT TGAGTATAAT
    GGATCATGTG GGTACCTCCT CAAACCAGAT TTCATGAGAC GGCCCGATCG AACCTTTGAT
    CCATTCTCTG AAACGCCTGT CGATGGGGTT ATTGCAGCCA CTTGCTCAGT GCAGGTCATA
    TCAGGTCAGT TTTTATCAGA TAAGAAGATT GGTACGTACG TAGAAGTGGA TATGTATGGG
    TTGCCCACCG ACACTATCCG TAAGGAATTC CGAACTCGCA TGGTGATGAA CAATGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TAATCCTCCC TGACCTGGCA
    GTCTTGAGAA TAGCAGTGTA TGACGACAAC AATAGGCTGA TAGGCCAGAG GATTCTTCCA
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATTTCCCTTC GAAACGAGGG AAATAAACCA
    TTATCACTGC CAACAATTTT CTGCAATATT GTTCTTAAAA CATATGTGCC TGATGGATTT
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC AGAAAAGAGA
    GCAGACCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TAGCAGATGT GCCCAGCGAT
    ACTTCTAAGA ATGACAAGAA AGGAAAAGCC AACACTGCCA AAGCAAATGT GACCCCACAG
    AGCAGCTCCG AGCTCAGACC AACCACCACA GCAGCCATGG GGTCTGGTGT GGAAGCCAAG
    AAAGGTATCG AACTTATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    CTGAAGCACT TAAAGAAGCA ACAGAAGGAG CTAAATTCTT TAAAGAAGAA ACATGCGAAG
    GAACACAGCA CCATGCAGAA GTTGCACTGC ACACAAGTTG ACAAAATTGT GGCACAGTAT
    GACAAAGAGA AGTCGACACA TGAGAAAATC CTAGAGAAGG CGATGAAGAA GAAGGGGGGA
    AGTAATTGTC TCGAAATGAA AAAAGAAACA GAAATTAAAA TTCAGACACT GACATCAGAT
    CACAAATCTA AGGTCAAAGA GATAGTGGCC CAGCACACAA AGGAGTGGTC AGAAATGATC
    AACACCCACA GTGCTGAGGA GCAAGAAATC CGGGACCTGC ACCTTAGCCA GCAGTGTGAG
    CTCCTGAGAA AGCTACTGAT CAGTGCCCAT GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAGGA AATGCGAGCA CACCAGGCTA AGATTTCTAT GGAAAACAGC
    AAAGCCATCA GCCAAGATAA ATCTATCAAG AACAAAGCAG AACGGGAAAG GCGAGTCAGG
    GAGTTAAATA GCAGCAACAC CAAAAAGTTT CTGGAAGAAA GAAAGAGACT TGCAATGAAA
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AACACCTAGA GTTCCTAGAG
    AAACAGAATG AGCAGGCGAA GGAGATGCAG CAGATGGTGA AATTGGAAGC CGAGATGGAC
    CGCAGACCAG CAACAGTAGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.