MADD cDNA ORF clone, Canis lupus familiaris(dog)

The following MADD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MADD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe294792 XM_022405458.1
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41235 XM_014121177.2
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41235 XM_014121178.2
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41235 XM_014121179.2
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41235 XM_014121180.2
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41236 XM_014121181.2
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41237 XM_014121182.2
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41238 XM_014121183.2
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41239 XM_014121184.2
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41240 XM_014121185.2
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41241 XM_014121186.2
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41242 XM_014121187.1
Latest version!
Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41243 XM_014121188.2
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41244 XM_014121189.2
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe20065 XM_005631241.3
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20066 XM_005631243.3
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20067 XM_533189.6
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20069 XM_855751.5
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20070 XM_005631246.3
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20071 XM_005631247.3
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20072 XM_855777.5
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20073 XM_014121190.2
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20074 XM_855872.5
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20075 XM_005631249.3
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20076 XM_005631250.3
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20077 XM_014121191.2
Latest version!
Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe20065 XM_005631241.2 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20071 XM_005631247.2 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20067 XM_533189.5 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20072 XM_855777.4 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20075 XM_005631249.2 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe41236 XM_014121181.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41237 XM_014121182.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41241 XM_014121186.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe20070 XM_005631246.2 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20066 XM_005631243.2 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20069 XM_855751.4 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe41238 XM_014121183.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe20073 XM_014121190.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe20076 XM_005631250.2 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe41239 XM_014121184.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41240 XM_014121185.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe20074 XM_855872.4 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe41235 XM_014121177.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41235 XM_014121178.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41235 XM_014121180.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41235 XM_014121179.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41243 XM_014121188.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe41244 XM_014121189.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe20077 XM_014121191.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe294792
    Clone ID Related Accession (Same CDS sequence) XM_022405458.1
    Accession Version XM_022405458.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTCTG GACTCACATG
    GCAGTAACTC AAACCTCGGC GCTCCAGAGG AGGGACTTAG AAGAGCCACC TCTGCAGAGA
    GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC
    TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT
    CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022261166.1
    CDS234..5186
    Translation

    Target ORF information:

    RefSeq Version XM_022405458.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022405458.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTCTG GACTCACATG
    GCAGTAACTC AAACCTCGGC GCTCCAGAGG AGGGACTTAG AAGAGCCACC TCTGCAGAGA
    GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC
    TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT
    CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41235
    Clone ID Related Accession (Same CDS sequence) XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
    Accession Version XM_014121178.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976653.1
    CDS185..5140
    Translation

    Target ORF information:

    RefSeq Version XM_014121178.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41235
    Clone ID Related Accession (Same CDS sequence) XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
    Accession Version XM_014121179.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976654.1
    CDS260..5215
    Translation

    Target ORF information:

    RefSeq Version XM_014121179.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121179.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41235
    Clone ID Related Accession (Same CDS sequence) XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
    Accession Version XM_014121180.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976655.1
    CDS105..5060
    Translation

    Target ORF information:

    RefSeq Version XM_014121180.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121180.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41235
    Clone ID Related Accession (Same CDS sequence) XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
    Accession Version XM_014121177.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976652.1
    CDS254..5209
    Translation

    Target ORF information:

    RefSeq Version XM_014121177.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121177.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41235
    Clone ID Related Accession (Same CDS sequence) XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
    Accession Version XM_014121177.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976652.1
    CDS234..5189
    Translation

    Target ORF information:

    RefSeq Version XM_014121177.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121177.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41235
    Clone ID Related Accession (Same CDS sequence) XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
    Accession Version XM_014121178.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121178.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976653.1
    CDS216..5171
    Translation

    Target ORF information:

    RefSeq Version XM_014121178.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121178.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41235
    Clone ID Related Accession (Same CDS sequence) XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
    Accession Version XM_014121180.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976655.1
    CDS206..5161
    Translation

    Target ORF information:

    RefSeq Version XM_014121180.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121180.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41235
    Clone ID Related Accession (Same CDS sequence) XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
    Accession Version XM_014121179.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121179.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976654.1
    CDS229..5184
    Translation

    Target ORF information:

    RefSeq Version XM_014121179.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121179.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41236
    Clone ID Related Accession (Same CDS sequence) XM_014121181.2 , XM_014121181.1
    Accession Version XM_014121181.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA
    GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA
    ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT
    ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG
    AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG
    GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG
    CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG
    TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT
    GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA
    AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT
    GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG
    GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC
    TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT
    CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976656.1
    CDS234..5186
    Translation

    Target ORF information:

    RefSeq Version XM_014121181.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121181.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA
    GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA
    ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT
    ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG
    AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG
    GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG
    CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG
    TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT
    GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA
    AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT
    GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG
    GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC
    TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT
    CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41236
    Clone ID Related Accession (Same CDS sequence) XM_014121181.2 , XM_014121181.1
    Accession Version XM_014121181.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA
    GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA
    ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT
    ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG
    AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG
    GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG
    CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG
    TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT
    GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA
    AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT
    GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG
    GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC
    TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT
    CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976656.1
    CDS254..5206
    Translation

    Target ORF information:

    RefSeq Version XM_014121181.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121181.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA
    GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA
    ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT
    ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG
    AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG
    GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG
    CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG
    TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT
    GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA
    AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT
    GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG
    GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC
    TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT
    CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41237
    Clone ID Related Accession (Same CDS sequence) XM_014121182.2 , XM_014121182.1
    Accession Version XM_014121182.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGTAAT
    AGTTCTGGAG AGACCCTTGG AGCAGATAGT GACCTGAGCA GCAATGCAGG TGATGGACCA
    GGTGGTGAAG GCAGCGCCCA CTTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT
    GAGACCAATT CTGCTACCAG CACCATCTTT GGGAAAGCCC ATAGCTTGAA GCCAAGTGTA
    AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCCGAAG ATGTAAGCCA ACGGGTCTAT
    CTGTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGATGCG
    GCTATGGAGA CCTTTTCTAT AAGCAAAGAA CGTTCTACTT TGTGGGACCA AATGCAGTTC
    TGGGAAGATG CATTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG
    GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTGGGAG AACATGACCG GAAGCGCCTA
    GAGGATGATG AAGATCGTTT GCTGGCCACA CTTTTGCACA ACCTCATCTC CTACATGCTG
    CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTAAT GGGAAAGTCC
    CATATTGGGC TTGTGTACAG CCAGCAAATC AATGAAGTGC TTGATCAGCT GGTGAACCTG
    AATGGACGTG ATCTCTCTAT CCGGTCCAGT GGCAGCCGGC ACATGAAAAA GCAGACATTT
    GTGGTACATG CAGGGACAGA CACAAATGGA GACATCTTCT TCATGGAGGT GTGTGATGAC
    TGTGTGGTGT TACGTAGTAA CATCGGAACG GTGTATGAGC GCTGGTGGTA TGAGAAGCTC
    ATCAACATGA CCTACTGTCC CAAGACCAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT
    GAGACTCAGC TCAACAAATT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGTGTGAAG
    GACAGCATGG AGCGGGCTGC TGCCCGGCAG CAAAGCATCA AACCTGGCCC TGAACTGGGT
    GGCGAGTTCC CTGTGCAGGA CATGAAGACT GGTGAGGGTG GCTTGCTGCA GGTCACCCTA
    GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA
    ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCATGAGAT CTGCTACTCT
    GTGTTGTGTC TCTTCTCGTA TGTGGCTGCA GTTCGTAGCA GTGAGGAAGA TCTCAGGACC
    CCACCCCGGC CCGTCTCGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976657.1
    CDS234..5177
    Translation

    Target ORF information:

    RefSeq Version XM_014121182.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121182.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGTAAT
    AGTTCTGGAG AGACCCTTGG AGCAGATAGT GACCTGAGCA GCAATGCAGG TGATGGACCA
    GGTGGTGAAG GCAGCGCCCA CTTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT
    GAGACCAATT CTGCTACCAG CACCATCTTT GGGAAAGCCC ATAGCTTGAA GCCAAGTGTA
    AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCCGAAG ATGTAAGCCA ACGGGTCTAT
    CTGTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGATGCG
    GCTATGGAGA CCTTTTCTAT AAGCAAAGAA CGTTCTACTT TGTGGGACCA AATGCAGTTC
    TGGGAAGATG CATTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG
    GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTGGGAG AACATGACCG GAAGCGCCTA
    GAGGATGATG AAGATCGTTT GCTGGCCACA CTTTTGCACA ACCTCATCTC CTACATGCTG
    CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTAAT GGGAAAGTCC
    CATATTGGGC TTGTGTACAG CCAGCAAATC AATGAAGTGC TTGATCAGCT GGTGAACCTG
    AATGGACGTG ATCTCTCTAT CCGGTCCAGT GGCAGCCGGC ACATGAAAAA GCAGACATTT
    GTGGTACATG CAGGGACAGA CACAAATGGA GACATCTTCT TCATGGAGGT GTGTGATGAC
    TGTGTGGTGT TACGTAGTAA CATCGGAACG GTGTATGAGC GCTGGTGGTA TGAGAAGCTC
    ATCAACATGA CCTACTGTCC CAAGACCAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT
    GAGACTCAGC TCAACAAATT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGTGTGAAG
    GACAGCATGG AGCGGGCTGC TGCCCGGCAG CAAAGCATCA AACCTGGCCC TGAACTGGGT
    GGCGAGTTCC CTGTGCAGGA CATGAAGACT GGTGAGGGTG GCTTGCTGCA GGTCACCCTA
    GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA
    ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCATGAGAT CTGCTACTCT
    GTGTTGTGTC TCTTCTCGTA TGTGGCTGCA GTTCGTAGCA GTGAGGAAGA TCTCAGGACC
    CCACCCCGGC CCGTCTCGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41237
    Clone ID Related Accession (Same CDS sequence) XM_014121182.2 , XM_014121182.1
    Accession Version XM_014121182.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGTAAT
    AGTTCTGGAG AGACCCTTGG AGCAGATAGT GACCTGAGCA GCAATGCAGG TGATGGACCA
    GGTGGTGAAG GCAGCGCCCA CTTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT
    GAGACCAATT CTGCTACCAG CACCATCTTT GGGAAAGCCC ATAGCTTGAA GCCAAGTGTA
    AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCCGAAG ATGTAAGCCA ACGGGTCTAT
    CTGTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGATGCG
    GCTATGGAGA CCTTTTCTAT AAGCAAAGAA CGTTCTACTT TGTGGGACCA AATGCAGTTC
    TGGGAAGATG CATTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG
    GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTGGGAG AACATGACCG GAAGCGCCTA
    GAGGATGATG AAGATCGTTT GCTGGCCACA CTTTTGCACA ACCTCATCTC CTACATGCTG
    CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTAAT GGGAAAGTCC
    CATATTGGGC TTGTGTACAG CCAGCAAATC AATGAAGTGC TTGATCAGCT GGTGAACCTG
    AATGGACGTG ATCTCTCTAT CCGGTCCAGT GGCAGCCGGC ACATGAAAAA GCAGACATTT
    GTGGTACATG CAGGGACAGA CACAAATGGA GACATCTTCT TCATGGAGGT GTGTGATGAC
    TGTGTGGTGT TACGTAGTAA CATCGGAACG GTGTATGAGC GCTGGTGGTA TGAGAAGCTC
    ATCAACATGA CCTACTGTCC CAAGACCAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT
    GAGACTCAGC TCAACAAATT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGTGTGAAG
    GACAGCATGG AGCGGGCTGC TGCCCGGCAG CAAAGCATCA AACCTGGCCC TGAACTGGGT
    GGCGAGTTCC CTGTGCAGGA CATGAAGACT GGTGAGGGTG GCTTGCTGCA GGTCACCCTA
    GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA
    ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCATGAGAT CTGCTACTCT
    GTGTTGTGTC TCTTCTCGTA TGTGGCTGCA GTTCGTAGCA GTGAGGAAGA TCTCAGGACC
    CCACCCCGGC CCGTCTCGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976657.1
    CDS254..5197
    Translation

    Target ORF information:

    RefSeq Version XM_014121182.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121182.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGTAAT
    AGTTCTGGAG AGACCCTTGG AGCAGATAGT GACCTGAGCA GCAATGCAGG TGATGGACCA
    GGTGGTGAAG GCAGCGCCCA CTTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT
    GAGACCAATT CTGCTACCAG CACCATCTTT GGGAAAGCCC ATAGCTTGAA GCCAAGTGTA
    AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCCGAAG ATGTAAGCCA ACGGGTCTAT
    CTGTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGATGCG
    GCTATGGAGA CCTTTTCTAT AAGCAAAGAA CGTTCTACTT TGTGGGACCA AATGCAGTTC
    TGGGAAGATG CATTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG
    GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTGGGAG AACATGACCG GAAGCGCCTA
    GAGGATGATG AAGATCGTTT GCTGGCCACA CTTTTGCACA ACCTCATCTC CTACATGCTG
    CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTAAT GGGAAAGTCC
    CATATTGGGC TTGTGTACAG CCAGCAAATC AATGAAGTGC TTGATCAGCT GGTGAACCTG
    AATGGACGTG ATCTCTCTAT CCGGTCCAGT GGCAGCCGGC ACATGAAAAA GCAGACATTT
    GTGGTACATG CAGGGACAGA CACAAATGGA GACATCTTCT TCATGGAGGT GTGTGATGAC
    TGTGTGGTGT TACGTAGTAA CATCGGAACG GTGTATGAGC GCTGGTGGTA TGAGAAGCTC
    ATCAACATGA CCTACTGTCC CAAGACCAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT
    GAGACTCAGC TCAACAAATT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGTGTGAAG
    GACAGCATGG AGCGGGCTGC TGCCCGGCAG CAAAGCATCA AACCTGGCCC TGAACTGGGT
    GGCGAGTTCC CTGTGCAGGA CATGAAGACT GGTGAGGGTG GCTTGCTGCA GGTCACCCTA
    GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA
    ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCATGAGAT CTGCTACTCT
    GTGTTGTGTC TCTTCTCGTA TGTGGCTGCA GTTCGTAGCA GTGAGGAAGA TCTCAGGACC
    CCACCCCGGC CCGTCTCGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41238
    Clone ID Related Accession (Same CDS sequence) XM_014121183.1 , XM_014121183.2
    Accession Version XM_014121183.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGCTTGAA ACTGGCAAGC GACTCAGACG CAGAGTCAGA CTCTCGAGCG
    AGCTCTCCCA CCTCCACCGT CTCCAACACC AGCACCGAGG GCTTCGGGGG CATCATGTCT
    TTTGCCAGCA GCCTGTATCG AAACCACAGT ACAAGCTTCA GTCTTTCAAA CCTCACACTG
    CCGACCAAAG GTGCCCGAGA GAAGACGACC CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA
    AATACTCTAA TGGAGATTGT TACTGAAGCC GGCCCCGGGA GTGGTGAAGG AAACAGGAGG
    GCCTTAGTGG ACCAGAAGTC ATCTGTTATT AAACACAGCC CAACAGTGAA AAGAGAGCCT
    CCATCACCTC AGGGTCGATC CAGCAATTCT AGTGAGAACC AGCAGTTCCT GAAGGAAGTG
    GTACACAGCG TGCTGGATGG CCAGGGTGTT GGCTGGCTCA ACATGAAAAA GGTGCGTCGG
    CTGCTGGAGA GTGAGCAGCT GCGAGTCTTT GTCCTGAGCA AGCTGAACCG AACTGTGCAG
    TCAGAAGATG ATGCCCGGCA GGACGTCATC CCCGATGTGG AGATCAGCAG AAAGGTTTAC
    AAGGGGATGT TAGACCTGCT CAAGTGCACA GTACTCAGCC TGGAGCAGTC CTATGCCCAC
    GCAGGTCTGG GTGGCATGGC CAGCATCTTT GGGCTTCTGG AGATTGCCCA GACCCACTAC
    TATAGTAAAG AACCAGACAA GCGGAAGAGA AGTCCAACAG AGAGTGTAAA TACACCAGTT
    GGCAAGGATC CTGGCCTGGC TGGGCGGGGG GACCCAAAAG CTATGGCACA GCTGAGAGTT
    CCCCAGCTGG GCCCTCGGGC GCCAAGTGCT GCAGGAAAGG GTCCCAAAGA ACTAGACACC
    AGAAGTTTAA AGGAAGAGAA TTTTGTAGCA TCTGTTGAAT TGTGGAACAA GCACCAGGAA
    GTGAAAAAGC AAAAAGCTTT GGAAAAACAG AGGCCTGAAG TCATCAAACC CGTCTTTGAC
    CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG ATCAGCGCAG ACAGTGGTGT GAGCCTGACA
    TCTGGTTCCC AGAGGACTGA TACAGACTCT GTCATTGGTG TGAGTCCAGC TGTTATGATC
    CGAAGCTCAA GTCAGGACTC TGAAGTTAGC ACCGTGGTGA GTAATAGTTC TGGAGAGACC
    CTTGGAGCAG ATAGTGACCT GAGCAGCAAT GCAGGTGATG GACCAGGTGG TGAAGGCAGC
    GCCCACTTGG CAAGCTCTCG GGGCACTTTG TCTGATAGTG AAATTGAGAC CAATTCTGCT
    ACCAGCACCA TCTTTGGGAA AGCCCATAGC TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG
    GGCAGCCCAG TTCGCTCTTC CGAAGATGTA AGCCAACGGG TCTATCTGTA CGAGGGACTC
    CTAGGAAGGG ACAAAGGATC GATGTGGGAC CAGTTAGAGG ATGCGGCTAT GGAGACCTTT
    TCTATAAGCA AAGAACGTTC TACTTTGTGG GACCAAATGC AGTTCTGGGA AGATGCATTC
    TTAGATGCTG TGATGTTGGA GAGAGAAGGG ATGGGTATGG ACCAGGGTCC CCAGGAAATG
    ATCGACAGGT ACCTGTCCCT GGGAGAACAT GACCGGAAGC GCCTAGAGGA TGATGAAGAT
    CGTTTGCTGG CCACACTTTT GCACAACCTC ATCTCCTACA TGCTGCTGAT GAAGGTAAAT
    AAGAATGACA TCCGGAAGAA GGTGAGGCGC CTAATGGGAA AGTCCCATAT TGGGCTTGTG
    TACAGCCAGC AAATCAATGA AGTGCTTGAT CAGCTGGTGA ACCTGAATGG ACGTGATCTC
    TCTATCCGGT CCAGTGGCAG CCGGCACATG AAAAAGCAGA CATTTGTGGT ACATGCAGGG
    ACAGACACAA ATGGAGACAT CTTCTTCATG GAGGTGTGTG ATGACTGTGT GGTGTTACGT
    AGTAACATCG GAACGGTGTA TGAGCGCTGG TGGTATGAGA AGCTCATCAA CATGACCTAC
    TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG CGTAGAAATG GCTCTGAGAC TCAGCTCAAC
    AAATTCTATA CTAAGAAGTG TCGGGAGCTG TACTACTGTG TGAAGGACAG CATGGAGCGG
    GCTGCTGCCC GGCAGCAAAG CATCAAACCT GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG
    CAGGACATGA AGACTGGTGA GGGTGGCTTG CTGCAGGTCA CCCTAGAAGG GATCAATCTC
    AAGTTCATGC ACAACCAGGA GCGGAAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC
    AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG
    AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC
    TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC
    TCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976658.1
    CDS255..5183
    Translation

    Target ORF information:

    RefSeq Version XM_014121183.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121183.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGCTTGAA ACTGGCAAGC GACTCAGACG CAGAGTCAGA CTCTCGAGCG
    AGCTCTCCCA CCTCCACCGT CTCCAACACC AGCACCGAGG GCTTCGGGGG CATCATGTCT
    TTTGCCAGCA GCCTGTATCG AAACCACAGT ACAAGCTTCA GTCTTTCAAA CCTCACACTG
    CCGACCAAAG GTGCCCGAGA GAAGACGACC CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA
    AATACTCTAA TGGAGATTGT TACTGAAGCC GGCCCCGGGA GTGGTGAAGG AAACAGGAGG
    GCCTTAGTGG ACCAGAAGTC ATCTGTTATT AAACACAGCC CAACAGTGAA AAGAGAGCCT
    CCATCACCTC AGGGTCGATC CAGCAATTCT AGTGAGAACC AGCAGTTCCT GAAGGAAGTG
    GTACACAGCG TGCTGGATGG CCAGGGTGTT GGCTGGCTCA ACATGAAAAA GGTGCGTCGG
    CTGCTGGAGA GTGAGCAGCT GCGAGTCTTT GTCCTGAGCA AGCTGAACCG AACTGTGCAG
    TCAGAAGATG ATGCCCGGCA GGACGTCATC CCCGATGTGG AGATCAGCAG AAAGGTTTAC
    AAGGGGATGT TAGACCTGCT CAAGTGCACA GTACTCAGCC TGGAGCAGTC CTATGCCCAC
    GCAGGTCTGG GTGGCATGGC CAGCATCTTT GGGCTTCTGG AGATTGCCCA GACCCACTAC
    TATAGTAAAG AACCAGACAA GCGGAAGAGA AGTCCAACAG AGAGTGTAAA TACACCAGTT
    GGCAAGGATC CTGGCCTGGC TGGGCGGGGG GACCCAAAAG CTATGGCACA GCTGAGAGTT
    CCCCAGCTGG GCCCTCGGGC GCCAAGTGCT GCAGGAAAGG GTCCCAAAGA ACTAGACACC
    AGAAGTTTAA AGGAAGAGAA TTTTGTAGCA TCTGTTGAAT TGTGGAACAA GCACCAGGAA
    GTGAAAAAGC AAAAAGCTTT GGAAAAACAG AGGCCTGAAG TCATCAAACC CGTCTTTGAC
    CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG ATCAGCGCAG ACAGTGGTGT GAGCCTGACA
    TCTGGTTCCC AGAGGACTGA TACAGACTCT GTCATTGGTG TGAGTCCAGC TGTTATGATC
    CGAAGCTCAA GTCAGGACTC TGAAGTTAGC ACCGTGGTGA GTAATAGTTC TGGAGAGACC
    CTTGGAGCAG ATAGTGACCT GAGCAGCAAT GCAGGTGATG GACCAGGTGG TGAAGGCAGC
    GCCCACTTGG CAAGCTCTCG GGGCACTTTG TCTGATAGTG AAATTGAGAC CAATTCTGCT
    ACCAGCACCA TCTTTGGGAA AGCCCATAGC TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG
    GGCAGCCCAG TTCGCTCTTC CGAAGATGTA AGCCAACGGG TCTATCTGTA CGAGGGACTC
    CTAGGAAGGG ACAAAGGATC GATGTGGGAC CAGTTAGAGG ATGCGGCTAT GGAGACCTTT
    TCTATAAGCA AAGAACGTTC TACTTTGTGG GACCAAATGC AGTTCTGGGA AGATGCATTC
    TTAGATGCTG TGATGTTGGA GAGAGAAGGG ATGGGTATGG ACCAGGGTCC CCAGGAAATG
    ATCGACAGGT ACCTGTCCCT GGGAGAACAT GACCGGAAGC GCCTAGAGGA TGATGAAGAT
    CGTTTGCTGG CCACACTTTT GCACAACCTC ATCTCCTACA TGCTGCTGAT GAAGGTAAAT
    AAGAATGACA TCCGGAAGAA GGTGAGGCGC CTAATGGGAA AGTCCCATAT TGGGCTTGTG
    TACAGCCAGC AAATCAATGA AGTGCTTGAT CAGCTGGTGA ACCTGAATGG ACGTGATCTC
    TCTATCCGGT CCAGTGGCAG CCGGCACATG AAAAAGCAGA CATTTGTGGT ACATGCAGGG
    ACAGACACAA ATGGAGACAT CTTCTTCATG GAGGTGTGTG ATGACTGTGT GGTGTTACGT
    AGTAACATCG GAACGGTGTA TGAGCGCTGG TGGTATGAGA AGCTCATCAA CATGACCTAC
    TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG CGTAGAAATG GCTCTGAGAC TCAGCTCAAC
    AAATTCTATA CTAAGAAGTG TCGGGAGCTG TACTACTGTG TGAAGGACAG CATGGAGCGG
    GCTGCTGCCC GGCAGCAAAG CATCAAACCT GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG
    CAGGACATGA AGACTGGTGA GGGTGGCTTG CTGCAGGTCA CCCTAGAAGG GATCAATCTC
    AAGTTCATGC ACAACCAGGA GCGGAAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC
    AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG
    AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC
    TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC
    TCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41238
    Clone ID Related Accession (Same CDS sequence) XM_014121183.1 , XM_014121183.2
    Accession Version XM_014121183.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGCTTGAA ACTGGCAAGC GACTCAGACG CAGAGTCAGA CTCTCGAGCG
    AGCTCTCCCA CCTCCACCGT CTCCAACACC AGCACCGAGG GCTTCGGGGG CATCATGTCT
    TTTGCCAGCA GCCTGTATCG AAACCACAGT ACAAGCTTCA GTCTTTCAAA CCTCACACTG
    CCGACCAAAG GTGCCCGAGA GAAGACGACC CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA
    AATACTCTAA TGGAGATTGT TACTGAAGCC GGCCCCGGGA GTGGTGAAGG AAACAGGAGG
    GCCTTAGTGG ACCAGAAGTC ATCTGTTATT AAACACAGCC CAACAGTGAA AAGAGAGCCT
    CCATCACCTC AGGGTCGATC CAGCAATTCT AGTGAGAACC AGCAGTTCCT GAAGGAAGTG
    GTACACAGCG TGCTGGATGG CCAGGGTGTT GGCTGGCTCA ACATGAAAAA GGTGCGTCGG
    CTGCTGGAGA GTGAGCAGCT GCGAGTCTTT GTCCTGAGCA AGCTGAACCG AACTGTGCAG
    TCAGAAGATG ATGCCCGGCA GGACGTCATC CCCGATGTGG AGATCAGCAG AAAGGTTTAC
    AAGGGGATGT TAGACCTGCT CAAGTGCACA GTACTCAGCC TGGAGCAGTC CTATGCCCAC
    GCAGGTCTGG GTGGCATGGC CAGCATCTTT GGGCTTCTGG AGATTGCCCA GACCCACTAC
    TATAGTAAAG AACCAGACAA GCGGAAGAGA AGTCCAACAG AGAGTGTAAA TACACCAGTT
    GGCAAGGATC CTGGCCTGGC TGGGCGGGGG GACCCAAAAG CTATGGCACA GCTGAGAGTT
    CCCCAGCTGG GCCCTCGGGC GCCAAGTGCT GCAGGAAAGG GTCCCAAAGA ACTAGACACC
    AGAAGTTTAA AGGAAGAGAA TTTTGTAGCA TCTGTTGAAT TGTGGAACAA GCACCAGGAA
    GTGAAAAAGC AAAAAGCTTT GGAAAAACAG AGGCCTGAAG TCATCAAACC CGTCTTTGAC
    CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG ATCAGCGCAG ACAGTGGTGT GAGCCTGACA
    TCTGGTTCCC AGAGGACTGA TACAGACTCT GTCATTGGTG TGAGTCCAGC TGTTATGATC
    CGAAGCTCAA GTCAGGACTC TGAAGTTAGC ACCGTGGTGA GTAATAGTTC TGGAGAGACC
    CTTGGAGCAG ATAGTGACCT GAGCAGCAAT GCAGGTGATG GACCAGGTGG TGAAGGCAGC
    GCCCACTTGG CAAGCTCTCG GGGCACTTTG TCTGATAGTG AAATTGAGAC CAATTCTGCT
    ACCAGCACCA TCTTTGGGAA AGCCCATAGC TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG
    GGCAGCCCAG TTCGCTCTTC CGAAGATGTA AGCCAACGGG TCTATCTGTA CGAGGGACTC
    CTAGGAAGGG ACAAAGGATC GATGTGGGAC CAGTTAGAGG ATGCGGCTAT GGAGACCTTT
    TCTATAAGCA AAGAACGTTC TACTTTGTGG GACCAAATGC AGTTCTGGGA AGATGCATTC
    TTAGATGCTG TGATGTTGGA GAGAGAAGGG ATGGGTATGG ACCAGGGTCC CCAGGAAATG
    ATCGACAGGT ACCTGTCCCT GGGAGAACAT GACCGGAAGC GCCTAGAGGA TGATGAAGAT
    CGTTTGCTGG CCACACTTTT GCACAACCTC ATCTCCTACA TGCTGCTGAT GAAGGTAAAT
    AAGAATGACA TCCGGAAGAA GGTGAGGCGC CTAATGGGAA AGTCCCATAT TGGGCTTGTG
    TACAGCCAGC AAATCAATGA AGTGCTTGAT CAGCTGGTGA ACCTGAATGG ACGTGATCTC
    TCTATCCGGT CCAGTGGCAG CCGGCACATG AAAAAGCAGA CATTTGTGGT ACATGCAGGG
    ACAGACACAA ATGGAGACAT CTTCTTCATG GAGGTGTGTG ATGACTGTGT GGTGTTACGT
    AGTAACATCG GAACGGTGTA TGAGCGCTGG TGGTATGAGA AGCTCATCAA CATGACCTAC
    TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG CGTAGAAATG GCTCTGAGAC TCAGCTCAAC
    AAATTCTATA CTAAGAAGTG TCGGGAGCTG TACTACTGTG TGAAGGACAG CATGGAGCGG
    GCTGCTGCCC GGCAGCAAAG CATCAAACCT GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG
    CAGGACATGA AGACTGGTGA GGGTGGCTTG CTGCAGGTCA CCCTAGAAGG GATCAATCTC
    AAGTTCATGC ACAACCAGGA GCGGAAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC
    AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG
    AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC
    TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC
    TCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976658.1
    CDS234..5162
    Translation

    Target ORF information:

    RefSeq Version XM_014121183.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121183.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGCTTGAA ACTGGCAAGC GACTCAGACG CAGAGTCAGA CTCTCGAGCG
    AGCTCTCCCA CCTCCACCGT CTCCAACACC AGCACCGAGG GCTTCGGGGG CATCATGTCT
    TTTGCCAGCA GCCTGTATCG AAACCACAGT ACAAGCTTCA GTCTTTCAAA CCTCACACTG
    CCGACCAAAG GTGCCCGAGA GAAGACGACC CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA
    AATACTCTAA TGGAGATTGT TACTGAAGCC GGCCCCGGGA GTGGTGAAGG AAACAGGAGG
    GCCTTAGTGG ACCAGAAGTC ATCTGTTATT AAACACAGCC CAACAGTGAA AAGAGAGCCT
    CCATCACCTC AGGGTCGATC CAGCAATTCT AGTGAGAACC AGCAGTTCCT GAAGGAAGTG
    GTACACAGCG TGCTGGATGG CCAGGGTGTT GGCTGGCTCA ACATGAAAAA GGTGCGTCGG
    CTGCTGGAGA GTGAGCAGCT GCGAGTCTTT GTCCTGAGCA AGCTGAACCG AACTGTGCAG
    TCAGAAGATG ATGCCCGGCA GGACGTCATC CCCGATGTGG AGATCAGCAG AAAGGTTTAC
    AAGGGGATGT TAGACCTGCT CAAGTGCACA GTACTCAGCC TGGAGCAGTC CTATGCCCAC
    GCAGGTCTGG GTGGCATGGC CAGCATCTTT GGGCTTCTGG AGATTGCCCA GACCCACTAC
    TATAGTAAAG AACCAGACAA GCGGAAGAGA AGTCCAACAG AGAGTGTAAA TACACCAGTT
    GGCAAGGATC CTGGCCTGGC TGGGCGGGGG GACCCAAAAG CTATGGCACA GCTGAGAGTT
    CCCCAGCTGG GCCCTCGGGC GCCAAGTGCT GCAGGAAAGG GTCCCAAAGA ACTAGACACC
    AGAAGTTTAA AGGAAGAGAA TTTTGTAGCA TCTGTTGAAT TGTGGAACAA GCACCAGGAA
    GTGAAAAAGC AAAAAGCTTT GGAAAAACAG AGGCCTGAAG TCATCAAACC CGTCTTTGAC
    CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG ATCAGCGCAG ACAGTGGTGT GAGCCTGACA
    TCTGGTTCCC AGAGGACTGA TACAGACTCT GTCATTGGTG TGAGTCCAGC TGTTATGATC
    CGAAGCTCAA GTCAGGACTC TGAAGTTAGC ACCGTGGTGA GTAATAGTTC TGGAGAGACC
    CTTGGAGCAG ATAGTGACCT GAGCAGCAAT GCAGGTGATG GACCAGGTGG TGAAGGCAGC
    GCCCACTTGG CAAGCTCTCG GGGCACTTTG TCTGATAGTG AAATTGAGAC CAATTCTGCT
    ACCAGCACCA TCTTTGGGAA AGCCCATAGC TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG
    GGCAGCCCAG TTCGCTCTTC CGAAGATGTA AGCCAACGGG TCTATCTGTA CGAGGGACTC
    CTAGGAAGGG ACAAAGGATC GATGTGGGAC CAGTTAGAGG ATGCGGCTAT GGAGACCTTT
    TCTATAAGCA AAGAACGTTC TACTTTGTGG GACCAAATGC AGTTCTGGGA AGATGCATTC
    TTAGATGCTG TGATGTTGGA GAGAGAAGGG ATGGGTATGG ACCAGGGTCC CCAGGAAATG
    ATCGACAGGT ACCTGTCCCT GGGAGAACAT GACCGGAAGC GCCTAGAGGA TGATGAAGAT
    CGTTTGCTGG CCACACTTTT GCACAACCTC ATCTCCTACA TGCTGCTGAT GAAGGTAAAT
    AAGAATGACA TCCGGAAGAA GGTGAGGCGC CTAATGGGAA AGTCCCATAT TGGGCTTGTG
    TACAGCCAGC AAATCAATGA AGTGCTTGAT CAGCTGGTGA ACCTGAATGG ACGTGATCTC
    TCTATCCGGT CCAGTGGCAG CCGGCACATG AAAAAGCAGA CATTTGTGGT ACATGCAGGG
    ACAGACACAA ATGGAGACAT CTTCTTCATG GAGGTGTGTG ATGACTGTGT GGTGTTACGT
    AGTAACATCG GAACGGTGTA TGAGCGCTGG TGGTATGAGA AGCTCATCAA CATGACCTAC
    TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG CGTAGAAATG GCTCTGAGAC TCAGCTCAAC
    AAATTCTATA CTAAGAAGTG TCGGGAGCTG TACTACTGTG TGAAGGACAG CATGGAGCGG
    GCTGCTGCCC GGCAGCAAAG CATCAAACCT GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG
    CAGGACATGA AGACTGGTGA GGGTGGCTTG CTGCAGGTCA CCCTAGAAGG GATCAATCTC
    AAGTTCATGC ACAACCAGGA GCGGAAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC
    AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG
    AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC
    TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC
    TCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41239
    Clone ID Related Accession (Same CDS sequence) XM_014121184.2 , XM_014121184.1
    Accession Version XM_014121184.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121184.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC
    AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT
    TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT
    AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT
    GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGAA GGGACAAAGG ATCGATGTGG
    GACCAGTTAG AGGATGCGGC TATGGAGACC TTTTCTATAA GCAAAGAACG TTCTACTTTG
    TGGGACCAAA TGCAGTTCTG GGAAGATGCA TTCTTAGATG CTGTGATGTT GGAGAGAGAA
    GGGATGGGTA TGGACCAGGG TCCCCAGGAA ATGATCGACA GGTACCTGTC CCTGGGAGAA
    CATGACCGGA AGCGCCTAGA GGATGATGAA GATCGTTTGC TGGCCACACT TTTGCACAAC
    CTCATCTCCT ACATGCTGCT GATGAAGGTA AATAAGAATG ACATCCGGAA GAAGGTGAGG
    CGCCTAATGG GAAAGTCCCA TATTGGGCTT GTGTACAGCC AGCAAATCAA TGAAGTGCTT
    GATCAGCTGG TGAACCTGAA TGGACGTGAT CTCTCTATCC GGTCCAGTGG CAGCCGGCAC
    ATGAAAAAGC AGACATTTGT GGTACATGCA GGGACAGACA CAAATGGAGA CATCTTCTTC
    ATGGAGGTGT GTGATGACTG TGTGGTGTTA CGTAGTAACA TCGGAACGGT GTATGAGCGC
    TGGTGGTATG AGAAGCTCAT CAACATGACC TACTGTCCCA AGACCAAGGT GTTGTGCTTG
    TGGCGTAGAA ATGGCTCTGA GACTCAGCTC AACAAATTCT ATACTAAGAA GTGTCGGGAG
    CTGTACTACT GTGTGAAGGA CAGCATGGAG CGGGCTGCTG CCCGGCAGCA AAGCATCAAA
    CCTGGCCCTG AACTGGGTGG CGAGTTCCCT GTGCAGGACA TGAAGACTGG TGAGGGTGGC
    TTGCTGCAGG TCACCCTAGA AGGGATCAAT CTCAAGTTCA TGCACAACCA GGAGCGGAAG
    GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TCCGAGGCGT CTTTGTCCTG
    GAGGAATTTG TTCCTGAAAT TAAAGAAGTG GTGAGCCACA AGTACAAGAC ACCAATGGCC
    CATGAGATCT GCTACTCTGT GTTGTGTCTC TTCTCGTATG TGGCTGCAGT TCGTAGCAGT
    GAGGAAGATC TCAGGACCCC ACCCCGGCCC GTCTCGAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976659.1
    CDS234..5135
    Translation

    Target ORF information:

    RefSeq Version XM_014121184.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121184.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC
    AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT
    TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT
    AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT
    GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGAA GGGACAAAGG ATCGATGTGG
    GACCAGTTAG AGGATGCGGC TATGGAGACC TTTTCTATAA GCAAAGAACG TTCTACTTTG
    TGGGACCAAA TGCAGTTCTG GGAAGATGCA TTCTTAGATG CTGTGATGTT GGAGAGAGAA
    GGGATGGGTA TGGACCAGGG TCCCCAGGAA ATGATCGACA GGTACCTGTC CCTGGGAGAA
    CATGACCGGA AGCGCCTAGA GGATGATGAA GATCGTTTGC TGGCCACACT TTTGCACAAC
    CTCATCTCCT ACATGCTGCT GATGAAGGTA AATAAGAATG ACATCCGGAA GAAGGTGAGG
    CGCCTAATGG GAAAGTCCCA TATTGGGCTT GTGTACAGCC AGCAAATCAA TGAAGTGCTT
    GATCAGCTGG TGAACCTGAA TGGACGTGAT CTCTCTATCC GGTCCAGTGG CAGCCGGCAC
    ATGAAAAAGC AGACATTTGT GGTACATGCA GGGACAGACA CAAATGGAGA CATCTTCTTC
    ATGGAGGTGT GTGATGACTG TGTGGTGTTA CGTAGTAACA TCGGAACGGT GTATGAGCGC
    TGGTGGTATG AGAAGCTCAT CAACATGACC TACTGTCCCA AGACCAAGGT GTTGTGCTTG
    TGGCGTAGAA ATGGCTCTGA GACTCAGCTC AACAAATTCT ATACTAAGAA GTGTCGGGAG
    CTGTACTACT GTGTGAAGGA CAGCATGGAG CGGGCTGCTG CCCGGCAGCA AAGCATCAAA
    CCTGGCCCTG AACTGGGTGG CGAGTTCCCT GTGCAGGACA TGAAGACTGG TGAGGGTGGC
    TTGCTGCAGG TCACCCTAGA AGGGATCAAT CTCAAGTTCA TGCACAACCA GGAGCGGAAG
    GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TCCGAGGCGT CTTTGTCCTG
    GAGGAATTTG TTCCTGAAAT TAAAGAAGTG GTGAGCCACA AGTACAAGAC ACCAATGGCC
    CATGAGATCT GCTACTCTGT GTTGTGTCTC TTCTCGTATG TGGCTGCAGT TCGTAGCAGT
    GAGGAAGATC TCAGGACCCC ACCCCGGCCC GTCTCGAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41239
    Clone ID Related Accession (Same CDS sequence) XM_014121184.2 , XM_014121184.1
    Accession Version XM_014121184.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC
    AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT
    TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT
    AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT
    GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGAA GGGACAAAGG ATCGATGTGG
    GACCAGTTAG AGGATGCGGC TATGGAGACC TTTTCTATAA GCAAAGAACG TTCTACTTTG
    TGGGACCAAA TGCAGTTCTG GGAAGATGCA TTCTTAGATG CTGTGATGTT GGAGAGAGAA
    GGGATGGGTA TGGACCAGGG TCCCCAGGAA ATGATCGACA GGTACCTGTC CCTGGGAGAA
    CATGACCGGA AGCGCCTAGA GGATGATGAA GATCGTTTGC TGGCCACACT TTTGCACAAC
    CTCATCTCCT ACATGCTGCT GATGAAGGTA AATAAGAATG ACATCCGGAA GAAGGTGAGG
    CGCCTAATGG GAAAGTCCCA TATTGGGCTT GTGTACAGCC AGCAAATCAA TGAAGTGCTT
    GATCAGCTGG TGAACCTGAA TGGACGTGAT CTCTCTATCC GGTCCAGTGG CAGCCGGCAC
    ATGAAAAAGC AGACATTTGT GGTACATGCA GGGACAGACA CAAATGGAGA CATCTTCTTC
    ATGGAGGTGT GTGATGACTG TGTGGTGTTA CGTAGTAACA TCGGAACGGT GTATGAGCGC
    TGGTGGTATG AGAAGCTCAT CAACATGACC TACTGTCCCA AGACCAAGGT GTTGTGCTTG
    TGGCGTAGAA ATGGCTCTGA GACTCAGCTC AACAAATTCT ATACTAAGAA GTGTCGGGAG
    CTGTACTACT GTGTGAAGGA CAGCATGGAG CGGGCTGCTG CCCGGCAGCA AAGCATCAAA
    CCTGGCCCTG AACTGGGTGG CGAGTTCCCT GTGCAGGACA TGAAGACTGG TGAGGGTGGC
    TTGCTGCAGG TCACCCTAGA AGGGATCAAT CTCAAGTTCA TGCACAACCA GGAGCGGAAG
    GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TCCGAGGCGT CTTTGTCCTG
    GAGGAATTTG TTCCTGAAAT TAAAGAAGTG GTGAGCCACA AGTACAAGAC ACCAATGGCC
    CATGAGATCT GCTACTCTGT GTTGTGTCTC TTCTCGTATG TGGCTGCAGT TCGTAGCAGT
    GAGGAAGATC TCAGGACCCC ACCCCGGCCC GTCTCGAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976659.1
    CDS254..5155
    Translation

    Target ORF information:

    RefSeq Version XM_014121184.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121184.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC
    AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT
    TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT
    AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT
    GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGAA GGGACAAAGG ATCGATGTGG
    GACCAGTTAG AGGATGCGGC TATGGAGACC TTTTCTATAA GCAAAGAACG TTCTACTTTG
    TGGGACCAAA TGCAGTTCTG GGAAGATGCA TTCTTAGATG CTGTGATGTT GGAGAGAGAA
    GGGATGGGTA TGGACCAGGG TCCCCAGGAA ATGATCGACA GGTACCTGTC CCTGGGAGAA
    CATGACCGGA AGCGCCTAGA GGATGATGAA GATCGTTTGC TGGCCACACT TTTGCACAAC
    CTCATCTCCT ACATGCTGCT GATGAAGGTA AATAAGAATG ACATCCGGAA GAAGGTGAGG
    CGCCTAATGG GAAAGTCCCA TATTGGGCTT GTGTACAGCC AGCAAATCAA TGAAGTGCTT
    GATCAGCTGG TGAACCTGAA TGGACGTGAT CTCTCTATCC GGTCCAGTGG CAGCCGGCAC
    ATGAAAAAGC AGACATTTGT GGTACATGCA GGGACAGACA CAAATGGAGA CATCTTCTTC
    ATGGAGGTGT GTGATGACTG TGTGGTGTTA CGTAGTAACA TCGGAACGGT GTATGAGCGC
    TGGTGGTATG AGAAGCTCAT CAACATGACC TACTGTCCCA AGACCAAGGT GTTGTGCTTG
    TGGCGTAGAA ATGGCTCTGA GACTCAGCTC AACAAATTCT ATACTAAGAA GTGTCGGGAG
    CTGTACTACT GTGTGAAGGA CAGCATGGAG CGGGCTGCTG CCCGGCAGCA AAGCATCAAA
    CCTGGCCCTG AACTGGGTGG CGAGTTCCCT GTGCAGGACA TGAAGACTGG TGAGGGTGGC
    TTGCTGCAGG TCACCCTAGA AGGGATCAAT CTCAAGTTCA TGCACAACCA GGAGCGGAAG
    GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TCCGAGGCGT CTTTGTCCTG
    GAGGAATTTG TTCCTGAAAT TAAAGAAGTG GTGAGCCACA AGTACAAGAC ACCAATGGCC
    CATGAGATCT GCTACTCTGT GTTGTGTCTC TTCTCGTATG TGGCTGCAGT TCGTAGCAGT
    GAGGAAGATC TCAGGACCCC ACCCCGGCCC GTCTCGAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41240
    Clone ID Related Accession (Same CDS sequence) XM_014121185.2 , XM_014121185.1
    Accession Version XM_014121185.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121185.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGCAAAGAAC GTTCTACTTT GTGGGACCAA
    ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT
    ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG
    AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG
    GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG
    CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG
    TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT
    GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA
    AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT
    GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG
    GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC
    TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT
    CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976660.1
    CDS234..5126
    Translation

    Target ORF information:

    RefSeq Version XM_014121185.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121185.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGCAAAGAAC GTTCTACTTT GTGGGACCAA
    ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT
    ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG
    AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG
    GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG
    CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG
    TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT
    GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA
    AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT
    GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG
    GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC
    TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT
    CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41240
    Clone ID Related Accession (Same CDS sequence) XM_014121185.2 , XM_014121185.1
    Accession Version XM_014121185.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGCAAAGAAC GTTCTACTTT GTGGGACCAA
    ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT
    ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG
    AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG
    GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG
    CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG
    TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT
    GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA
    AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT
    GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG
    GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC
    TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT
    CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976660.1
    CDS255..5147
    Translation

    Target ORF information:

    RefSeq Version XM_014121185.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121185.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGCAAAGAAC GTTCTACTTT GTGGGACCAA
    ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT
    ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG
    AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG
    GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG
    CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG
    TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT
    GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA
    AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT
    GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG
    GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT
    GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC
    TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT
    CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41241
    Clone ID Related Accession (Same CDS sequence) XM_014121186.1 , XM_014121186.2
    Accession Version XM_014121186.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT
    GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGAATTG
    TGGAACAAGC ACCAGGAAGT GAAAAAGCAA AAAGCTTTGG AAAAACAGAG GCCTGAAGTC
    ATCAAACCCG TCTTTGACCT TGGTGAGACA GAGGAGAAAA AGTCCCAGAT CAGCGCAGAC
    AGTGGTGTGA GCCTGACATC TGGTTCCCAG AGGACTGATA CAGACTCTGT CATTGGTGTG
    AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGACTCTG AAGTTAGCAC CGTGGTGAGT
    AATAGTTCTG GAGAGACCCT TGGAGCAGAT AGTGACCTGA GCAGCAATGC AGGTGATGGA
    CCAGGTGGTG AAGGCAGCGC CCACTTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA
    ATTGAGACCA ATTCTGCTAC CAGCACCATC TTTGGGAAAG CCCATAGCTT GAAGCCAAGT
    GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCCG AAGATGTAAG CCAACGGGTC
    TATCTGTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAT
    GCGGCTATGG AGACCTTTTC TATAAGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG
    TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC
    CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC
    CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC
    CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA
    TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT
    GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG
    CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC
    TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG
    AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG
    GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC
    CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC
    TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG
    ACCCCACCCC GGCCCGTCTC GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976661.1
    CDS254..5080
    Translation

    Target ORF information:

    RefSeq Version XM_014121186.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121186.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT
    GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGAATTG
    TGGAACAAGC ACCAGGAAGT GAAAAAGCAA AAAGCTTTGG AAAAACAGAG GCCTGAAGTC
    ATCAAACCCG TCTTTGACCT TGGTGAGACA GAGGAGAAAA AGTCCCAGAT CAGCGCAGAC
    AGTGGTGTGA GCCTGACATC TGGTTCCCAG AGGACTGATA CAGACTCTGT CATTGGTGTG
    AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGACTCTG AAGTTAGCAC CGTGGTGAGT
    AATAGTTCTG GAGAGACCCT TGGAGCAGAT AGTGACCTGA GCAGCAATGC AGGTGATGGA
    CCAGGTGGTG AAGGCAGCGC CCACTTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA
    ATTGAGACCA ATTCTGCTAC CAGCACCATC TTTGGGAAAG CCCATAGCTT GAAGCCAAGT
    GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCCG AAGATGTAAG CCAACGGGTC
    TATCTGTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAT
    GCGGCTATGG AGACCTTTTC TATAAGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG
    TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC
    CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC
    CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC
    CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA
    TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT
    GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG
    CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC
    TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG
    AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG
    GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC
    CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC
    TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG
    ACCCCACCCC GGCCCGTCTC GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41241
    Clone ID Related Accession (Same CDS sequence) XM_014121186.1 , XM_014121186.2
    Accession Version XM_014121186.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121186.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT
    GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGAATTG
    TGGAACAAGC ACCAGGAAGT GAAAAAGCAA AAAGCTTTGG AAAAACAGAG GCCTGAAGTC
    ATCAAACCCG TCTTTGACCT TGGTGAGACA GAGGAGAAAA AGTCCCAGAT CAGCGCAGAC
    AGTGGTGTGA GCCTGACATC TGGTTCCCAG AGGACTGATA CAGACTCTGT CATTGGTGTG
    AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGACTCTG AAGTTAGCAC CGTGGTGAGT
    AATAGTTCTG GAGAGACCCT TGGAGCAGAT AGTGACCTGA GCAGCAATGC AGGTGATGGA
    CCAGGTGGTG AAGGCAGCGC CCACTTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA
    ATTGAGACCA ATTCTGCTAC CAGCACCATC TTTGGGAAAG CCCATAGCTT GAAGCCAAGT
    GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCCG AAGATGTAAG CCAACGGGTC
    TATCTGTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAT
    GCGGCTATGG AGACCTTTTC TATAAGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG
    TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC
    CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC
    CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC
    CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA
    TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT
    GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG
    CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC
    TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG
    AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG
    GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC
    CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC
    TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG
    ACCCCACCCC GGCCCGTCTC GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976661.1
    CDS234..5060
    Translation

    Target ORF information:

    RefSeq Version XM_014121186.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121186.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT
    GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGAATTG
    TGGAACAAGC ACCAGGAAGT GAAAAAGCAA AAAGCTTTGG AAAAACAGAG GCCTGAAGTC
    ATCAAACCCG TCTTTGACCT TGGTGAGACA GAGGAGAAAA AGTCCCAGAT CAGCGCAGAC
    AGTGGTGTGA GCCTGACATC TGGTTCCCAG AGGACTGATA CAGACTCTGT CATTGGTGTG
    AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGACTCTG AAGTTAGCAC CGTGGTGAGT
    AATAGTTCTG GAGAGACCCT TGGAGCAGAT AGTGACCTGA GCAGCAATGC AGGTGATGGA
    CCAGGTGGTG AAGGCAGCGC CCACTTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA
    ATTGAGACCA ATTCTGCTAC CAGCACCATC TTTGGGAAAG CCCATAGCTT GAAGCCAAGT
    GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCCG AAGATGTAAG CCAACGGGTC
    TATCTGTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAT
    GCGGCTATGG AGACCTTTTC TATAAGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG
    TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC
    CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC
    CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC
    CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA
    TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT
    GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG
    CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC
    TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG
    AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG
    GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC
    CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC
    TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG
    ACCCCACCCC GGCCCGTCTC GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41242
    Clone ID Related Accession (Same CDS sequence) XM_014121187.1
    Accession Version XM_014121187.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTCTG GACTCACATG
    GCAGTAACTC AAACCTCGGC GCTCCAGAGG AGGGACTTAG AAGAGCCACC TCTGCAGAGA
    GGTCAGGAGG CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976662.1
    CDS255..5069
    Translation

    Target ORF information:

    RefSeq Version XM_014121187.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121187.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTCTG GACTCACATG
    GCAGTAACTC AAACCTCGGC GCTCCAGAGG AGGGACTTAG AAGAGCCACC TCTGCAGAGA
    GGTCAGGAGG CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41243
    Clone ID Related Accession (Same CDS sequence) XM_014121188.1 , XM_014121188.2
    Accession Version XM_014121188.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGCTTGA AACTGGCAAG CGACTCAGAC GCAGAGTCAG ACTCTCGAGC GAGCTCTCCC
    ACCTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC
    AGCCTGTATC GAAACCACAG TACAAGCTTC AGTCTTTCAA ACCTCACACT GCCGACCAAA
    GGTGCCCGAG AGAAGACGAC CCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA
    ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCCTTAGTG
    GACCAGAAGT CATCTGTTAT TAAACACAGC CCAACAGTGA AAAGAGAGCC TCCATCACCT
    CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAAGT GGTACACAGC
    GTGCTGGATG GCCAGGGTGT TGGCTGGCTC AACATGAAAA AGGTGCGTCG GCTGCTGGAG
    AGTGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GAACTGTGCA GTCAGAAGAT
    GATGCCCGGC AGGACGTCAT CCCCGATGTG GAGATCAGCA GAAAGGTTTA CAAGGGGATG
    TTAGACCTGC TCAAGTGCAC AGTACTCAGC CTGGAGCAGT CCTATGCCCA CGCAGGTCTG
    GGTGGCATGG CCAGCATCTT TGGGCTTCTG GAGATTGCCC AGACCCACTA CTATAGTAAA
    GAACCAGACA AGCGGAAGAG AAGTCCAACA GAGAGTGTAA ATACACCAGT TGGCAAGGAT
    CCTGGCCTGG CTGGGCGGGG GGACCCAAAA GCTATGGCAC AGCTGAGAGT TCCCCAGCTG
    GGCCCTCGGG CGCCAAGTGC TGCAGGAAAG GGTCCCAAAG AACTAGACAC CAGAAGTTTA
    AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG
    CAAAAAGCTT TGGAAAAACA GAGGCCTGAA GTCATCAAAC CCGTCTTTGA CCTTGGTGAG
    ACAGAGGAGA AAAAGTCCCA GATCAGCGCA GACAGTGGTG TGAGCCTGAC ATCTGGTTCC
    CAGAGGACTG ATACAGACTC TGTCATTGGT GTGAGTCCAG CTGTTATGAT CCGAAGCTCA
    AGTCAGGACT CTGAAGTTAG CACCGTGGTG AGTAATAGTT CTGGAGAGAC CCTTGGAGCA
    GATAGTGACC TGAGCAGCAA TGCAGGTGAT GGACCAGGTG GTGAAGGCAG CGCCCACTTG
    GCAAGCTCTC GGGGCACTTT GTCTGATAGT GAAATTGAGA CCAATTCTGC TACCAGCACC
    ATCTTTGGGA AAGCCCATAG CTTGAAGCCA AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA
    GTTCGCTCTT CCGAAGATGT AAGCCAACGG GTCTATCTGT ACGAGGGACT CCTAGGAAGG
    GACAAAGGAT CGATGTGGGA CCAGTTAGAG GATGCGGCTA TGGAGACCTT TTCTATAAGC
    AAAGAACGTT CTACTTTGTG GGACCAAATG CAGTTCTGGG AAGATGCATT CTTAGATGCT
    GTGATGTTGG AGAGAGAAGG GATGGGTATG GACCAGGGTC CCCAGGAAAT GATCGACAGG
    TACCTGTCCC TGGGAGAACA TGACCGGAAG CGCCTAGAGG ATGATGAAGA TCGTTTGCTG
    GCCACACTTT TGCACAACCT CATCTCCTAC ATGCTGCTGA TGAAGGTAAA TAAGAATGAC
    ATCCGGAAGA AGGTGAGGCG CCTAATGGGA AAGTCCCATA TTGGGCTTGT GTACAGCCAG
    CAAATCAATG AAGTGCTTGA TCAGCTGGTG AACCTGAATG GACGTGATCT CTCTATCCGG
    TCCAGTGGCA GCCGGCACAT GAAAAAGCAG ACATTTGTGG TACATGCAGG GACAGACACA
    AATGGAGACA TCTTCTTCAT GGAGGTGTGT GATGACTGTG TGGTGTTACG TAGTAACATC
    GGAACGGTGT ATGAGCGCTG GTGGTATGAG AAGCTCATCA ACATGACCTA CTGTCCCAAG
    ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT GGCTCTGAGA CTCAGCTCAA CAAATTCTAT
    ACTAAGAAGT GTCGGGAGCT GTACTACTGT GTGAAGGACA GCATGGAGCG GGCTGCTGCC
    CGGCAGCAAA GCATCAAACC TGGCCCTGAA CTGGGTGGCG AGTTCCCTGT GCAGGACATG
    AAGACTGGTG AGGGTGGCTT GCTGCAGGTC ACCCTAGAAG GGATCAATCT CAAGTTCATG
    CACAACCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTC
    CGAGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAATTA AAGAAGTGGT GAGCCACAAG
    TACAAGACAC CAATGGCCCA TGAGATCTGC TACTCTGTGT TGTGTCTCTT CTCGTATGTG
    GCTGCAGTTC GTAGCAGTGA GGAAGATCTC AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976663.1
    CDS254..5053
    Translation

    Target ORF information:

    RefSeq Version XM_014121188.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121188.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGCTTGA AACTGGCAAG CGACTCAGAC GCAGAGTCAG ACTCTCGAGC GAGCTCTCCC
    ACCTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC
    AGCCTGTATC GAAACCACAG TACAAGCTTC AGTCTTTCAA ACCTCACACT GCCGACCAAA
    GGTGCCCGAG AGAAGACGAC CCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA
    ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCCTTAGTG
    GACCAGAAGT CATCTGTTAT TAAACACAGC CCAACAGTGA AAAGAGAGCC TCCATCACCT
    CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAAGT GGTACACAGC
    GTGCTGGATG GCCAGGGTGT TGGCTGGCTC AACATGAAAA AGGTGCGTCG GCTGCTGGAG
    AGTGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GAACTGTGCA GTCAGAAGAT
    GATGCCCGGC AGGACGTCAT CCCCGATGTG GAGATCAGCA GAAAGGTTTA CAAGGGGATG
    TTAGACCTGC TCAAGTGCAC AGTACTCAGC CTGGAGCAGT CCTATGCCCA CGCAGGTCTG
    GGTGGCATGG CCAGCATCTT TGGGCTTCTG GAGATTGCCC AGACCCACTA CTATAGTAAA
    GAACCAGACA AGCGGAAGAG AAGTCCAACA GAGAGTGTAA ATACACCAGT TGGCAAGGAT
    CCTGGCCTGG CTGGGCGGGG GGACCCAAAA GCTATGGCAC AGCTGAGAGT TCCCCAGCTG
    GGCCCTCGGG CGCCAAGTGC TGCAGGAAAG GGTCCCAAAG AACTAGACAC CAGAAGTTTA
    AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG
    CAAAAAGCTT TGGAAAAACA GAGGCCTGAA GTCATCAAAC CCGTCTTTGA CCTTGGTGAG
    ACAGAGGAGA AAAAGTCCCA GATCAGCGCA GACAGTGGTG TGAGCCTGAC ATCTGGTTCC
    CAGAGGACTG ATACAGACTC TGTCATTGGT GTGAGTCCAG CTGTTATGAT CCGAAGCTCA
    AGTCAGGACT CTGAAGTTAG CACCGTGGTG AGTAATAGTT CTGGAGAGAC CCTTGGAGCA
    GATAGTGACC TGAGCAGCAA TGCAGGTGAT GGACCAGGTG GTGAAGGCAG CGCCCACTTG
    GCAAGCTCTC GGGGCACTTT GTCTGATAGT GAAATTGAGA CCAATTCTGC TACCAGCACC
    ATCTTTGGGA AAGCCCATAG CTTGAAGCCA AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA
    GTTCGCTCTT CCGAAGATGT AAGCCAACGG GTCTATCTGT ACGAGGGACT CCTAGGAAGG
    GACAAAGGAT CGATGTGGGA CCAGTTAGAG GATGCGGCTA TGGAGACCTT TTCTATAAGC
    AAAGAACGTT CTACTTTGTG GGACCAAATG CAGTTCTGGG AAGATGCATT CTTAGATGCT
    GTGATGTTGG AGAGAGAAGG GATGGGTATG GACCAGGGTC CCCAGGAAAT GATCGACAGG
    TACCTGTCCC TGGGAGAACA TGACCGGAAG CGCCTAGAGG ATGATGAAGA TCGTTTGCTG
    GCCACACTTT TGCACAACCT CATCTCCTAC ATGCTGCTGA TGAAGGTAAA TAAGAATGAC
    ATCCGGAAGA AGGTGAGGCG CCTAATGGGA AAGTCCCATA TTGGGCTTGT GTACAGCCAG
    CAAATCAATG AAGTGCTTGA TCAGCTGGTG AACCTGAATG GACGTGATCT CTCTATCCGG
    TCCAGTGGCA GCCGGCACAT GAAAAAGCAG ACATTTGTGG TACATGCAGG GACAGACACA
    AATGGAGACA TCTTCTTCAT GGAGGTGTGT GATGACTGTG TGGTGTTACG TAGTAACATC
    GGAACGGTGT ATGAGCGCTG GTGGTATGAG AAGCTCATCA ACATGACCTA CTGTCCCAAG
    ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT GGCTCTGAGA CTCAGCTCAA CAAATTCTAT
    ACTAAGAAGT GTCGGGAGCT GTACTACTGT GTGAAGGACA GCATGGAGCG GGCTGCTGCC
    CGGCAGCAAA GCATCAAACC TGGCCCTGAA CTGGGTGGCG AGTTCCCTGT GCAGGACATG
    AAGACTGGTG AGGGTGGCTT GCTGCAGGTC ACCCTAGAAG GGATCAATCT CAAGTTCATG
    CACAACCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTC
    CGAGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAATTA AAGAAGTGGT GAGCCACAAG
    TACAAGACAC CAATGGCCCA TGAGATCTGC TACTCTGTGT TGTGTCTCTT CTCGTATGTG
    GCTGCAGTTC GTAGCAGTGA GGAAGATCTC AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41243
    Clone ID Related Accession (Same CDS sequence) XM_014121188.1 , XM_014121188.2
    Accession Version XM_014121188.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121188.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGCTTGA AACTGGCAAG CGACTCAGAC GCAGAGTCAG ACTCTCGAGC GAGCTCTCCC
    ACCTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC
    AGCCTGTATC GAAACCACAG TACAAGCTTC AGTCTTTCAA ACCTCACACT GCCGACCAAA
    GGTGCCCGAG AGAAGACGAC CCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA
    ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCCTTAGTG
    GACCAGAAGT CATCTGTTAT TAAACACAGC CCAACAGTGA AAAGAGAGCC TCCATCACCT
    CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAAGT GGTACACAGC
    GTGCTGGATG GCCAGGGTGT TGGCTGGCTC AACATGAAAA AGGTGCGTCG GCTGCTGGAG
    AGTGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GAACTGTGCA GTCAGAAGAT
    GATGCCCGGC AGGACGTCAT CCCCGATGTG GAGATCAGCA GAAAGGTTTA CAAGGGGATG
    TTAGACCTGC TCAAGTGCAC AGTACTCAGC CTGGAGCAGT CCTATGCCCA CGCAGGTCTG
    GGTGGCATGG CCAGCATCTT TGGGCTTCTG GAGATTGCCC AGACCCACTA CTATAGTAAA
    GAACCAGACA AGCGGAAGAG AAGTCCAACA GAGAGTGTAA ATACACCAGT TGGCAAGGAT
    CCTGGCCTGG CTGGGCGGGG GGACCCAAAA GCTATGGCAC AGCTGAGAGT TCCCCAGCTG
    GGCCCTCGGG CGCCAAGTGC TGCAGGAAAG GGTCCCAAAG AACTAGACAC CAGAAGTTTA
    AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG
    CAAAAAGCTT TGGAAAAACA GAGGCCTGAA GTCATCAAAC CCGTCTTTGA CCTTGGTGAG
    ACAGAGGAGA AAAAGTCCCA GATCAGCGCA GACAGTGGTG TGAGCCTGAC ATCTGGTTCC
    CAGAGGACTG ATACAGACTC TGTCATTGGT GTGAGTCCAG CTGTTATGAT CCGAAGCTCA
    AGTCAGGACT CTGAAGTTAG CACCGTGGTG AGTAATAGTT CTGGAGAGAC CCTTGGAGCA
    GATAGTGACC TGAGCAGCAA TGCAGGTGAT GGACCAGGTG GTGAAGGCAG CGCCCACTTG
    GCAAGCTCTC GGGGCACTTT GTCTGATAGT GAAATTGAGA CCAATTCTGC TACCAGCACC
    ATCTTTGGGA AAGCCCATAG CTTGAAGCCA AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA
    GTTCGCTCTT CCGAAGATGT AAGCCAACGG GTCTATCTGT ACGAGGGACT CCTAGGAAGG
    GACAAAGGAT CGATGTGGGA CCAGTTAGAG GATGCGGCTA TGGAGACCTT TTCTATAAGC
    AAAGAACGTT CTACTTTGTG GGACCAAATG CAGTTCTGGG AAGATGCATT CTTAGATGCT
    GTGATGTTGG AGAGAGAAGG GATGGGTATG GACCAGGGTC CCCAGGAAAT GATCGACAGG
    TACCTGTCCC TGGGAGAACA TGACCGGAAG CGCCTAGAGG ATGATGAAGA TCGTTTGCTG
    GCCACACTTT TGCACAACCT CATCTCCTAC ATGCTGCTGA TGAAGGTAAA TAAGAATGAC
    ATCCGGAAGA AGGTGAGGCG CCTAATGGGA AAGTCCCATA TTGGGCTTGT GTACAGCCAG
    CAAATCAATG AAGTGCTTGA TCAGCTGGTG AACCTGAATG GACGTGATCT CTCTATCCGG
    TCCAGTGGCA GCCGGCACAT GAAAAAGCAG ACATTTGTGG TACATGCAGG GACAGACACA
    AATGGAGACA TCTTCTTCAT GGAGGTGTGT GATGACTGTG TGGTGTTACG TAGTAACATC
    GGAACGGTGT ATGAGCGCTG GTGGTATGAG AAGCTCATCA ACATGACCTA CTGTCCCAAG
    ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT GGCTCTGAGA CTCAGCTCAA CAAATTCTAT
    ACTAAGAAGT GTCGGGAGCT GTACTACTGT GTGAAGGACA GCATGGAGCG GGCTGCTGCC
    CGGCAGCAAA GCATCAAACC TGGCCCTGAA CTGGGTGGCG AGTTCCCTGT GCAGGACATG
    AAGACTGGTG AGGGTGGCTT GCTGCAGGTC ACCCTAGAAG GGATCAATCT CAAGTTCATG
    CACAACCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTC
    CGAGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAATTA AAGAAGTGGT GAGCCACAAG
    TACAAGACAC CAATGGCCCA TGAGATCTGC TACTCTGTGT TGTGTCTCTT CTCGTATGTG
    GCTGCAGTTC GTAGCAGTGA GGAAGATCTC AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976663.1
    CDS234..5033
    Translation

    Target ORF information:

    RefSeq Version XM_014121188.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121188.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGCTTGA AACTGGCAAG CGACTCAGAC GCAGAGTCAG ACTCTCGAGC GAGCTCTCCC
    ACCTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC
    AGCCTGTATC GAAACCACAG TACAAGCTTC AGTCTTTCAA ACCTCACACT GCCGACCAAA
    GGTGCCCGAG AGAAGACGAC CCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA
    ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCCTTAGTG
    GACCAGAAGT CATCTGTTAT TAAACACAGC CCAACAGTGA AAAGAGAGCC TCCATCACCT
    CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAAGT GGTACACAGC
    GTGCTGGATG GCCAGGGTGT TGGCTGGCTC AACATGAAAA AGGTGCGTCG GCTGCTGGAG
    AGTGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GAACTGTGCA GTCAGAAGAT
    GATGCCCGGC AGGACGTCAT CCCCGATGTG GAGATCAGCA GAAAGGTTTA CAAGGGGATG
    TTAGACCTGC TCAAGTGCAC AGTACTCAGC CTGGAGCAGT CCTATGCCCA CGCAGGTCTG
    GGTGGCATGG CCAGCATCTT TGGGCTTCTG GAGATTGCCC AGACCCACTA CTATAGTAAA
    GAACCAGACA AGCGGAAGAG AAGTCCAACA GAGAGTGTAA ATACACCAGT TGGCAAGGAT
    CCTGGCCTGG CTGGGCGGGG GGACCCAAAA GCTATGGCAC AGCTGAGAGT TCCCCAGCTG
    GGCCCTCGGG CGCCAAGTGC TGCAGGAAAG GGTCCCAAAG AACTAGACAC CAGAAGTTTA
    AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG
    CAAAAAGCTT TGGAAAAACA GAGGCCTGAA GTCATCAAAC CCGTCTTTGA CCTTGGTGAG
    ACAGAGGAGA AAAAGTCCCA GATCAGCGCA GACAGTGGTG TGAGCCTGAC ATCTGGTTCC
    CAGAGGACTG ATACAGACTC TGTCATTGGT GTGAGTCCAG CTGTTATGAT CCGAAGCTCA
    AGTCAGGACT CTGAAGTTAG CACCGTGGTG AGTAATAGTT CTGGAGAGAC CCTTGGAGCA
    GATAGTGACC TGAGCAGCAA TGCAGGTGAT GGACCAGGTG GTGAAGGCAG CGCCCACTTG
    GCAAGCTCTC GGGGCACTTT GTCTGATAGT GAAATTGAGA CCAATTCTGC TACCAGCACC
    ATCTTTGGGA AAGCCCATAG CTTGAAGCCA AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA
    GTTCGCTCTT CCGAAGATGT AAGCCAACGG GTCTATCTGT ACGAGGGACT CCTAGGAAGG
    GACAAAGGAT CGATGTGGGA CCAGTTAGAG GATGCGGCTA TGGAGACCTT TTCTATAAGC
    AAAGAACGTT CTACTTTGTG GGACCAAATG CAGTTCTGGG AAGATGCATT CTTAGATGCT
    GTGATGTTGG AGAGAGAAGG GATGGGTATG GACCAGGGTC CCCAGGAAAT GATCGACAGG
    TACCTGTCCC TGGGAGAACA TGACCGGAAG CGCCTAGAGG ATGATGAAGA TCGTTTGCTG
    GCCACACTTT TGCACAACCT CATCTCCTAC ATGCTGCTGA TGAAGGTAAA TAAGAATGAC
    ATCCGGAAGA AGGTGAGGCG CCTAATGGGA AAGTCCCATA TTGGGCTTGT GTACAGCCAG
    CAAATCAATG AAGTGCTTGA TCAGCTGGTG AACCTGAATG GACGTGATCT CTCTATCCGG
    TCCAGTGGCA GCCGGCACAT GAAAAAGCAG ACATTTGTGG TACATGCAGG GACAGACACA
    AATGGAGACA TCTTCTTCAT GGAGGTGTGT GATGACTGTG TGGTGTTACG TAGTAACATC
    GGAACGGTGT ATGAGCGCTG GTGGTATGAG AAGCTCATCA ACATGACCTA CTGTCCCAAG
    ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT GGCTCTGAGA CTCAGCTCAA CAAATTCTAT
    ACTAAGAAGT GTCGGGAGCT GTACTACTGT GTGAAGGACA GCATGGAGCG GGCTGCTGCC
    CGGCAGCAAA GCATCAAACC TGGCCCTGAA CTGGGTGGCG AGTTCCCTGT GCAGGACATG
    AAGACTGGTG AGGGTGGCTT GCTGCAGGTC ACCCTAGAAG GGATCAATCT CAAGTTCATG
    CACAACCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTC
    CGAGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAATTA AAGAAGTGGT GAGCCACAAG
    TACAAGACAC CAATGGCCCA TGAGATCTGC TACTCTGTGT TGTGTCTCTT CTCGTATGTG
    GCTGCAGTTC GTAGCAGTGA GGAAGATCTC AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41244
    Clone ID Related Accession (Same CDS sequence) XM_014121189.1 , XM_014121189.2
    Accession Version XM_014121189.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4746bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X16
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA
    GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA
    ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT
    ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG
    AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG
    GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG
    CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG
    TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT
    GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA
    AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT
    GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG
    GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGTTCCTGAA ATTAAAGAAG
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976664.1
    CDS254..4999
    Translation

    Target ORF information:

    RefSeq Version XM_014121189.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121189.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA
    GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA
    ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT
    ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG
    AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG
    GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG
    CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG
    TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT
    GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA
    AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT
    GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG
    GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGTTCCTGAA ATTAAAGAAG
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe41244
    Clone ID Related Accession (Same CDS sequence) XM_014121189.1 , XM_014121189.2
    Accession Version XM_014121189.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4746bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121189.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA
    GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA
    ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT
    ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG
    AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG
    GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG
    CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG
    TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT
    GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA
    AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT
    GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG
    GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGTTCCTGAA ATTAAAGAAG
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976664.1
    CDS234..4979
    Translation

    Target ORF information:

    RefSeq Version XM_014121189.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121189.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA
    GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA
    ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT
    ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG
    AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC
    TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG
    GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG
    GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG
    CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG
    TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT
    GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA
    AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC
    TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT
    GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG
    GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGTTCCTGAA ATTAAAGAAG
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20065
    Clone ID Related Accession (Same CDS sequence) XM_005631241.2 , XM_005631241.3
    Accession Version XM_005631241.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631241.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC
    TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG
    ACCCCACCCC GGCCCGTCTC GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631298.1
    CDS254..5200
    Translation

    Target ORF information:

    RefSeq Version XM_005631241.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631241.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC
    TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG
    ACCCCACCCC GGCCCGTCTC GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20065
    Clone ID Related Accession (Same CDS sequence) XM_005631241.2 , XM_005631241.3
    Accession Version XM_005631241.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631241.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC
    TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG
    ACCCCACCCC GGCCCGTCTC GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631298.1
    CDS234..5180
    Translation

    Target ORF information:

    RefSeq Version XM_005631241.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631241.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC
    TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG
    ACCCCACCCC GGCCCGTCTC GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20066
    Clone ID Related Accession (Same CDS sequence) XM_005631243.3 , XM_005631243.2
    Accession Version XM_005631243.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631243.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631300.1
    CDS234..5129
    Translation

    Target ORF information:

    RefSeq Version XM_005631243.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631243.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20066
    Clone ID Related Accession (Same CDS sequence) XM_005631243.3 , XM_005631243.2
    Accession Version XM_005631243.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631243.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631300.1
    CDS254..5149
    Translation

    Target ORF information:

    RefSeq Version XM_005631243.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631243.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA
    TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG
    ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA
    GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20067
    Clone ID Related Accession (Same CDS sequence) XM_533189.5 , XM_533189.6
    Accession Version XM_533189.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_533189.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC
    AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT
    TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT
    AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT
    GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG
    GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG
    ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT
    GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC
    ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT
    CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG
    AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG
    GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG
    TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG
    CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT
    GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG
    CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC
    TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC
    AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_533189.2
    CDS255..5084
    Translation

    Target ORF information:

    RefSeq Version XM_533189.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_533189.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC
    AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT
    TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT
    AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT
    GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG
    GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG
    ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT
    GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC
    ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT
    CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG
    AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG
    GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG
    TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG
    CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT
    GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG
    CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC
    TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC
    AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20067
    Clone ID Related Accession (Same CDS sequence) XM_533189.5 , XM_533189.6
    Accession Version XM_533189.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_533189.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC
    AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT
    TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT
    AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT
    GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG
    GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG
    ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT
    GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC
    ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT
    CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG
    AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG
    GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG
    TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG
    CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT
    GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG
    CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC
    TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC
    AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_533189.2
    CDS234..5063
    Translation

    Target ORF information:

    RefSeq Version XM_533189.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_533189.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC
    AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT
    TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT
    AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT
    GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG
    GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG
    ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT
    GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC
    ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT
    CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG
    AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG
    GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG
    TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG
    CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT
    GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG
    CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC
    TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC
    AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20069
    Clone ID Related Accession (Same CDS sequence) XM_855751.5 , XM_855751.4
    Accession Version XM_855751.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4770bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_855751.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC
    AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT
    TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT
    AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT
    GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG
    GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG
    ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT
    GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC
    ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT
    CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG
    AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG
    GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG
    TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG
    CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT
    GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG
    CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC
    TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC
    AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_860844.1
    CDS234..5003
    Translation

    Target ORF information:

    RefSeq Version XM_855751.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_855751.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC
    AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT
    TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT
    AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT
    GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG
    GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG
    ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT
    GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC
    ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT
    CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG
    AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG
    GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG
    TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG
    CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT
    GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG
    CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC
    TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC
    AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20069
    Clone ID Related Accession (Same CDS sequence) XM_855751.5 , XM_855751.4
    Accession Version XM_855751.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4770bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_855751.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC
    AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT
    TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT
    AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT
    GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG
    GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG
    ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT
    GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC
    ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT
    CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG
    AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG
    GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG
    TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG
    CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT
    GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG
    CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC
    TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC
    AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_860844.1
    CDS255..5024
    Translation

    Target ORF information:

    RefSeq Version XM_855751.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_855751.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC
    AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT
    TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT
    AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT
    GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG
    GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG
    ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT
    GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC
    ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC
    CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT
    CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG
    AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG
    GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG
    TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG
    CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG
    TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT
    GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG
    CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT
    CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC
    TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC
    AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20070
    Clone ID Related Accession (Same CDS sequence) XM_005631246.2 , XM_005631246.3
    Accession Version XM_005631246.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4749bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X15
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631246.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG
    AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631303.1
    CDS254..5002
    Translation

    Target ORF information:

    RefSeq Version XM_005631246.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631246.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG
    AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20070
    Clone ID Related Accession (Same CDS sequence) XM_005631246.2 , XM_005631246.3
    Accession Version XM_005631246.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4749bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631246.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG
    AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631303.1
    CDS234..4982
    Translation

    Target ORF information:

    RefSeq Version XM_005631246.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631246.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC
    CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG
    AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20071
    Clone ID Related Accession (Same CDS sequence) XM_005631247.3 , XM_005631247.2
    Accession Version XM_005631247.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631247.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGA GTAATAGTTC TGGAGAGACC CTTGGAGCAG ATAGTGACCT GAGCAGCAAT
    GCAGGTGATG GACCAGGTGG TGAAGGCAGC GCCCACTTGG CAAGCTCTCG GGGCACTTTG
    TCTGATAGTG AAATTGAGAC CAATTCTGCT ACCAGCACCA TCTTTGGGAA AGCCCATAGC
    TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTCTTC CGAAGATGTA
    AGCCAACGGG TCTATCTGTA CGAGGGACTC CTAGGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC
    TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG
    ACCCCACCCC GGCCCGTCTC GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631304.1
    CDS234..5000
    Translation

    Target ORF information:

    RefSeq Version XM_005631247.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631247.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGA GTAATAGTTC TGGAGAGACC CTTGGAGCAG ATAGTGACCT GAGCAGCAAT
    GCAGGTGATG GACCAGGTGG TGAAGGCAGC GCCCACTTGG CAAGCTCTCG GGGCACTTTG
    TCTGATAGTG AAATTGAGAC CAATTCTGCT ACCAGCACCA TCTTTGGGAA AGCCCATAGC
    TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTCTTC CGAAGATGTA
    AGCCAACGGG TCTATCTGTA CGAGGGACTC CTAGGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC
    TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG
    ACCCCACCCC GGCCCGTCTC GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20071
    Clone ID Related Accession (Same CDS sequence) XM_005631247.3 , XM_005631247.2
    Accession Version XM_005631247.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGA GTAATAGTTC TGGAGAGACC CTTGGAGCAG ATAGTGACCT GAGCAGCAAT
    GCAGGTGATG GACCAGGTGG TGAAGGCAGC GCCCACTTGG CAAGCTCTCG GGGCACTTTG
    TCTGATAGTG AAATTGAGAC CAATTCTGCT ACCAGCACCA TCTTTGGGAA AGCCCATAGC
    TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTCTTC CGAAGATGTA
    AGCCAACGGG TCTATCTGTA CGAGGGACTC CTAGGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC
    TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG
    ACCCCACCCC GGCCCGTCTC GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631304.1
    CDS255..5021
    Translation

    Target ORF information:

    RefSeq Version XM_005631247.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631247.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC
    CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC
    TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT
    AGCACCGTGA GTAATAGTTC TGGAGAGACC CTTGGAGCAG ATAGTGACCT GAGCAGCAAT
    GCAGGTGATG GACCAGGTGG TGAAGGCAGC GCCCACTTGG CAAGCTCTCG GGGCACTTTG
    TCTGATAGTG AAATTGAGAC CAATTCTGCT ACCAGCACCA TCTTTGGGAA AGCCCATAGC
    TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTCTTC CGAAGATGTA
    AGCCAACGGG TCTATCTGTA CGAGGGACTC CTAGGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT
    GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC
    TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG
    ACCCCACCCC GGCCCGTCTC GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20072
    Clone ID Related Accession (Same CDS sequence) XM_855777.4 , XM_855777.5
    Accession Version XM_855777.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X17
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_855777.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT
    GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG
    CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG
    GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG
    CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG
    AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG
    ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT
    GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG
    AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT
    GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT
    GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA
    CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC
    ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT
    AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG
    TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA
    CCCCGGCCCG TCTCGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_860870.1
    CDS255..4955
    Translation

    Target ORF information:

    RefSeq Version XM_855777.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_855777.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT
    GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG
    CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG
    GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG
    CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG
    AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG
    ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT
    GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG
    AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT
    GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT
    GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA
    CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC
    ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT
    AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG
    TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA
    CCCCGGCCCG TCTCGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20072
    Clone ID Related Accession (Same CDS sequence) XM_855777.4 , XM_855777.5
    Accession Version XM_855777.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_855777.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT
    GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG
    CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG
    GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG
    CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG
    AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG
    ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT
    GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG
    AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT
    GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT
    GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA
    CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC
    ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT
    AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG
    TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA
    CCCCGGCCCG TCTCGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_860870.1
    CDS234..4934
    Translation

    Target ORF information:

    RefSeq Version XM_855777.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_855777.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT
    GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG
    CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG
    GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG
    CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG
    AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG
    ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT
    GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG
    AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT
    GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT
    GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA
    CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC
    ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT
    AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG
    TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA
    CCCCGGCCCG TCTCGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20073
    Clone ID Related Accession (Same CDS sequence) XM_014121190.1 , XM_014121190.2
    Accession Version XM_014121190.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4689bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X18
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG
    AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976665.1
    CDS254..4942
    Translation

    Target ORF information:

    RefSeq Version XM_014121190.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121190.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG
    AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20073
    Clone ID Related Accession (Same CDS sequence) XM_014121190.1 , XM_014121190.2
    Accession Version XM_014121190.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4689bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121190.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG
    AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976665.1
    CDS234..4922
    Translation

    Target ORF information:

    RefSeq Version XM_014121190.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121190.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT
    CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT
    GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC
    TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC
    ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA
    AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC
    TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA
    CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT
    GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC
    TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC
    CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC
    GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA
    CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG
    CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT
    CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC
    CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA
    GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT
    GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG
    CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC
    AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC
    ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC
    GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA
    GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT
    GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG
    AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC
    CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG
    TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC
    CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG
    GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC
    CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC
    TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA
    ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG
    CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA
    AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG
    GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG
    TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT
    AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC
    TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC
    CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG
    CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG
    AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20074
    Clone ID Related Accession (Same CDS sequence) XM_855872.5 , XM_855872.4
    Accession Version XM_855872.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_855872.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG
    CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG
    GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG
    CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG
    AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG
    ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT
    GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG
    AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT
    GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT
    GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA
    CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC
    ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT
    AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG
    TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA
    CCCCGGCCCG TCTCGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_860965.1
    CDS234..4874
    Translation

    Target ORF information:

    RefSeq Version XM_855872.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_855872.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG
    CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG
    GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG
    CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG
    AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG
    ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT
    GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG
    AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT
    GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT
    GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA
    CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC
    ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT
    AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG
    TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA
    CCCCGGCCCG TCTCGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20074
    Clone ID Related Accession (Same CDS sequence) XM_855872.5 , XM_855872.4
    Accession Version XM_855872.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X19
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_855872.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG
    CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG
    GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG
    CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG
    AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG
    ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT
    GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG
    AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT
    GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT
    GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA
    CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC
    ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT
    AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG
    TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA
    CCCCGGCCCG TCTCGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_860965.1
    CDS254..4894
    Translation

    Target ORF information:

    RefSeq Version XM_855872.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_855872.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG
    CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG
    GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG
    CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG
    AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG
    ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT
    GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG
    AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT
    GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT
    GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA
    CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC
    ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT
    AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG
    TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA
    CCCCGGCCCG TCTCGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20075
    Clone ID Related Accession (Same CDS sequence) XM_005631249.2 , XM_005631249.3
    Accession Version XM_005631249.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X21
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631249.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT GAGTAATAGT
    TCTGGAGAGA CCCTTGGAGC AGATAGTGAC CTGAGCAGCA ATGCAGGTGA TGGACCAGGT
    GGTGAAGGCA GCGCCCACTT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG
    ACCAATTCTG CTACCAGCAC CATCTTTGGG AAAGCCCATA GCTTGAAGCC AAGTGTAAAG
    GAGAAGCTGG TGGGCAGCCC AGTTCGCTCT TCCGAAGATG TAAGCCAACG GGTCTATCTG
    TACGAGGGAC TCCTAGGCAA AGAACGTTCT ACTTTGTGGG ACCAAATGCA GTTCTGGGAA
    GATGCATTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC
    CAGGAAATGA TCGACAGGTA CCTGTCCCTG GGAGAACATG ACCGGAAGCG CCTAGAGGAT
    GATGAAGATC GTTTGCTGGC CACACTTTTG CACAACCTCA TCTCCTACAT GCTGCTGATG
    AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TAATGGGAAA GTCCCATATT
    GGGCTTGTGT ACAGCCAGCA AATCAATGAA GTGCTTGATC AGCTGGTGAA CCTGAATGGA
    CGTGATCTCT CTATCCGGTC CAGTGGCAGC CGGCACATGA AAAAGCAGAC ATTTGTGGTA
    CATGCAGGGA CAGACACAAA TGGAGACATC TTCTTCATGG AGGTGTGTGA TGACTGTGTG
    GTGTTACGTA GTAACATCGG AACGGTGTAT GAGCGCTGGT GGTATGAGAA GCTCATCAAC
    ATGACCTACT GTCCCAAGAC CAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACT
    CAGCTCAACA AATTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC
    ATGGAGCGGG CTGCTGCCCG GCAGCAAAGC ATCAAACCTG GCCCTGAACT GGGTGGCGAG
    TTCCCTGTGC AGGACATGAA GACTGGTGAG GGTGGCTTGC TGCAGGTCAC CCTAGAAGGG
    ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC
    AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG
    AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC
    TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC
    TCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631306.1
    CDS254..4882
    Translation

    Target ORF information:

    RefSeq Version XM_005631249.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631249.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT GAGTAATAGT
    TCTGGAGAGA CCCTTGGAGC AGATAGTGAC CTGAGCAGCA ATGCAGGTGA TGGACCAGGT
    GGTGAAGGCA GCGCCCACTT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG
    ACCAATTCTG CTACCAGCAC CATCTTTGGG AAAGCCCATA GCTTGAAGCC AAGTGTAAAG
    GAGAAGCTGG TGGGCAGCCC AGTTCGCTCT TCCGAAGATG TAAGCCAACG GGTCTATCTG
    TACGAGGGAC TCCTAGGCAA AGAACGTTCT ACTTTGTGGG ACCAAATGCA GTTCTGGGAA
    GATGCATTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC
    CAGGAAATGA TCGACAGGTA CCTGTCCCTG GGAGAACATG ACCGGAAGCG CCTAGAGGAT
    GATGAAGATC GTTTGCTGGC CACACTTTTG CACAACCTCA TCTCCTACAT GCTGCTGATG
    AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TAATGGGAAA GTCCCATATT
    GGGCTTGTGT ACAGCCAGCA AATCAATGAA GTGCTTGATC AGCTGGTGAA CCTGAATGGA
    CGTGATCTCT CTATCCGGTC CAGTGGCAGC CGGCACATGA AAAAGCAGAC ATTTGTGGTA
    CATGCAGGGA CAGACACAAA TGGAGACATC TTCTTCATGG AGGTGTGTGA TGACTGTGTG
    GTGTTACGTA GTAACATCGG AACGGTGTAT GAGCGCTGGT GGTATGAGAA GCTCATCAAC
    ATGACCTACT GTCCCAAGAC CAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACT
    CAGCTCAACA AATTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC
    ATGGAGCGGG CTGCTGCCCG GCAGCAAAGC ATCAAACCTG GCCCTGAACT GGGTGGCGAG
    TTCCCTGTGC AGGACATGAA GACTGGTGAG GGTGGCTTGC TGCAGGTCAC CCTAGAAGGG
    ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC
    AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG
    AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC
    TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC
    TCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20075
    Clone ID Related Accession (Same CDS sequence) XM_005631249.2 , XM_005631249.3
    Accession Version XM_005631249.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631249.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT GAGTAATAGT
    TCTGGAGAGA CCCTTGGAGC AGATAGTGAC CTGAGCAGCA ATGCAGGTGA TGGACCAGGT
    GGTGAAGGCA GCGCCCACTT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG
    ACCAATTCTG CTACCAGCAC CATCTTTGGG AAAGCCCATA GCTTGAAGCC AAGTGTAAAG
    GAGAAGCTGG TGGGCAGCCC AGTTCGCTCT TCCGAAGATG TAAGCCAACG GGTCTATCTG
    TACGAGGGAC TCCTAGGCAA AGAACGTTCT ACTTTGTGGG ACCAAATGCA GTTCTGGGAA
    GATGCATTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC
    CAGGAAATGA TCGACAGGTA CCTGTCCCTG GGAGAACATG ACCGGAAGCG CCTAGAGGAT
    GATGAAGATC GTTTGCTGGC CACACTTTTG CACAACCTCA TCTCCTACAT GCTGCTGATG
    AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TAATGGGAAA GTCCCATATT
    GGGCTTGTGT ACAGCCAGCA AATCAATGAA GTGCTTGATC AGCTGGTGAA CCTGAATGGA
    CGTGATCTCT CTATCCGGTC CAGTGGCAGC CGGCACATGA AAAAGCAGAC ATTTGTGGTA
    CATGCAGGGA CAGACACAAA TGGAGACATC TTCTTCATGG AGGTGTGTGA TGACTGTGTG
    GTGTTACGTA GTAACATCGG AACGGTGTAT GAGCGCTGGT GGTATGAGAA GCTCATCAAC
    ATGACCTACT GTCCCAAGAC CAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACT
    CAGCTCAACA AATTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC
    ATGGAGCGGG CTGCTGCCCG GCAGCAAAGC ATCAAACCTG GCCCTGAACT GGGTGGCGAG
    TTCCCTGTGC AGGACATGAA GACTGGTGAG GGTGGCTTGC TGCAGGTCAC CCTAGAAGGG
    ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC
    AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG
    AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC
    TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC
    TCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631306.1
    CDS234..4862
    Translation

    Target ORF information:

    RefSeq Version XM_005631249.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631249.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT GAGTAATAGT
    TCTGGAGAGA CCCTTGGAGC AGATAGTGAC CTGAGCAGCA ATGCAGGTGA TGGACCAGGT
    GGTGAAGGCA GCGCCCACTT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG
    ACCAATTCTG CTACCAGCAC CATCTTTGGG AAAGCCCATA GCTTGAAGCC AAGTGTAAAG
    GAGAAGCTGG TGGGCAGCCC AGTTCGCTCT TCCGAAGATG TAAGCCAACG GGTCTATCTG
    TACGAGGGAC TCCTAGGCAA AGAACGTTCT ACTTTGTGGG ACCAAATGCA GTTCTGGGAA
    GATGCATTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC
    CAGGAAATGA TCGACAGGTA CCTGTCCCTG GGAGAACATG ACCGGAAGCG CCTAGAGGAT
    GATGAAGATC GTTTGCTGGC CACACTTTTG CACAACCTCA TCTCCTACAT GCTGCTGATG
    AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TAATGGGAAA GTCCCATATT
    GGGCTTGTGT ACAGCCAGCA AATCAATGAA GTGCTTGATC AGCTGGTGAA CCTGAATGGA
    CGTGATCTCT CTATCCGGTC CAGTGGCAGC CGGCACATGA AAAAGCAGAC ATTTGTGGTA
    CATGCAGGGA CAGACACAAA TGGAGACATC TTCTTCATGG AGGTGTGTGA TGACTGTGTG
    GTGTTACGTA GTAACATCGG AACGGTGTAT GAGCGCTGGT GGTATGAGAA GCTCATCAAC
    ATGACCTACT GTCCCAAGAC CAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACT
    CAGCTCAACA AATTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC
    ATGGAGCGGG CTGCTGCCCG GCAGCAAAGC ATCAAACCTG GCCCTGAACT GGGTGGCGAG
    TTCCCTGTGC AGGACATGAA GACTGGTGAG GGTGGCTTGC TGCAGGTCAC CCTAGAAGGG
    ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC
    AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG
    AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC
    TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC
    TCGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20076
    Clone ID Related Accession (Same CDS sequence) XM_005631250.3 , XM_005631250.2
    Accession Version XM_005631250.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631250.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    AGTAATAGTT CTGGAGAGAC CCTTGGAGCA GATAGTGACC TGAGCAGCAA TGCAGGTGAT
    GGACCAGGTG GTGAAGGCAG CGCCCACTTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT
    GAAATTGAGA CCAATTCTGC TACCAGCACC ATCTTTGGGA AAGCCCATAG CTTGAAGCCA
    AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CCGAAGATGT AAGCCAACGG
    GTCTATCTGT ACGAGGGACT CCTAGGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG
    TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC
    CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC
    CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC
    CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA
    TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT
    GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG
    CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC
    TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG
    AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG
    GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC
    CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT
    AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA
    GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCATG AGATCTGCTA CTCTGTGTTG
    TGTCTCTTCT CGTATGTGGC TGCAGTTCGT AGCAGTGAGG AAGATCTCAG GACCCCACCC
    CGGCCCGTCT CGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631307.1
    CDS234..4871
    Translation

    Target ORF information:

    RefSeq Version XM_005631250.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631250.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    AGTAATAGTT CTGGAGAGAC CCTTGGAGCA GATAGTGACC TGAGCAGCAA TGCAGGTGAT
    GGACCAGGTG GTGAAGGCAG CGCCCACTTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT
    GAAATTGAGA CCAATTCTGC TACCAGCACC ATCTTTGGGA AAGCCCATAG CTTGAAGCCA
    AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CCGAAGATGT AAGCCAACGG
    GTCTATCTGT ACGAGGGACT CCTAGGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG
    TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC
    CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC
    CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC
    CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA
    TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT
    GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG
    CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC
    TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG
    AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG
    GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC
    CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT
    AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA
    GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCATG AGATCTGCTA CTCTGTGTTG
    TGTCTCTTCT CGTATGTGGC TGCAGTTCGT AGCAGTGAGG AAGATCTCAG GACCCCACCC
    CGGCCCGTCT CGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20076
    Clone ID Related Accession (Same CDS sequence) XM_005631250.3 , XM_005631250.2
    Accession Version XM_005631250.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X20
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    AGTAATAGTT CTGGAGAGAC CCTTGGAGCA GATAGTGACC TGAGCAGCAA TGCAGGTGAT
    GGACCAGGTG GTGAAGGCAG CGCCCACTTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT
    GAAATTGAGA CCAATTCTGC TACCAGCACC ATCTTTGGGA AAGCCCATAG CTTGAAGCCA
    AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CCGAAGATGT AAGCCAACGG
    GTCTATCTGT ACGAGGGACT CCTAGGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG
    TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC
    CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC
    CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC
    CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA
    TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT
    GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG
    CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC
    TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG
    AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG
    GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC
    CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT
    AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA
    GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCATG AGATCTGCTA CTCTGTGTTG
    TGTCTCTTCT CGTATGTGGC TGCAGTTCGT AGCAGTGAGG AAGATCTCAG GACCCCACCC
    CGGCCCGTCT CGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005631307.1
    CDS255..4892
    Translation

    Target ORF information:

    RefSeq Version XM_005631250.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005631250.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    AGTAATAGTT CTGGAGAGAC CCTTGGAGCA GATAGTGACC TGAGCAGCAA TGCAGGTGAT
    GGACCAGGTG GTGAAGGCAG CGCCCACTTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT
    GAAATTGAGA CCAATTCTGC TACCAGCACC ATCTTTGGGA AAGCCCATAG CTTGAAGCCA
    AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CCGAAGATGT AAGCCAACGG
    GTCTATCTGT ACGAGGGACT CCTAGGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG
    TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC
    CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC
    CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG
    CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG
    TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC
    CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA
    TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT
    GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG
    CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC
    TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG
    AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG
    GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC
    CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT
    AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA
    GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCATG AGATCTGCTA CTCTGTGTTG
    TGTCTCTTCT CGTATGTGGC TGCAGTTCGT AGCAGTGAGG AAGATCTCAG GACCCCACCC
    CGGCCCGTCT CGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20077
    Clone ID Related Accession (Same CDS sequence) XM_014121191.1 , XM_014121191.2
    Accession Version XM_014121191.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4443bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product MAP kinase-activating death domain protein isoform X22
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG
    CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG
    GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG
    CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG
    AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG
    ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT
    GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG
    AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT
    GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT
    GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA
    CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC
    ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976666.1
    CDS254..4696
    Translation

    Target ORF information:

    RefSeq Version XM_014121191.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121191.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG
    CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG
    GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG
    CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG
    AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG
    ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT
    GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG
    AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT
    GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT
    GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA
    CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC
    ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe20077
    Clone ID Related Accession (Same CDS sequence) XM_014121191.1 , XM_014121191.2
    Accession Version XM_014121191.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4443bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product MAP kinase-activating death domain protein isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121191.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG
    CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG
    GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG
    CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG
    AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG
    ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT
    GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG
    AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT
    GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT
    GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA
    CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC
    ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976666.1
    CDS234..4676
    Translation

    Target ORF information:

    RefSeq Version XM_014121191.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014121191.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
    AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG
    GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC
    TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC
    CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC
    TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA
    GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG
    TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC
    AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG
    CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG
    GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA
    AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC
    CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG
    CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT
    CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT
    CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG
    CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT
    GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC
    ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC
    AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG
    GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG
    GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC
    CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT
    AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC
    AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC
    CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT
    GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT
    GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC
    AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG
    CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC
    CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT
    GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT
    GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA
    TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG
    AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC
    TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT
    GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC
    CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA
    GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA
    GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC
    TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT
    CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT
    CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA
    ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG
    CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC
    ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG
    CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG
    ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG
    GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG
    ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG
    AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT
    ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT
    CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT
    GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC
    GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT
    GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG
    GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT
    GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT
    AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG
    CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA
    CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG
    CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG
    GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG
    CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC
    ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA
    AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG
    AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG
    ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT
    GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG
    AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT
    GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT
    GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA
    CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC
    ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.