MADD cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MADD
Entrez Gene ID	475982
Full Name	MAP kinase activating death domain
General protein information	Preferred Names MAP kinase activating death domain Names MAP kinase-activating death domain protein
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014121178.1	XP_013976653.1	MAP kinase-activating death domain protein isoform X1
XM_005631241.2	XP_005631298.1	MAP kinase-activating death domain protein isoform X3
XM_533189.5	XP_533189.2	MAP kinase-activating death domain protein isoform X9
XM_014121188.1	XP_013976663.1	MAP kinase-activating death domain protein isoform X12
XM_014121191.1	XP_013976666.1	MAP kinase-activating death domain protein isoform X22
XM_014121181.2	XP_013976656.1	MAP kinase-activating death domain protein isoform X2
XM_022405458.1	XP_022261166.1	MAP kinase-activating death domain protein isoform X3
XM_005631241.3	XP_005631298.1	MAP kinase-activating death domain protein isoform X4
XM_005631247.3	XP_005631304.1	MAP kinase-activating death domain protein isoform X14
XM_005631250.3	XP_005631307.1	MAP kinase-activating death domain protein isoform X20
XM_014121179.1	XP_013976654.1	MAP kinase-activating death domain protein isoform X1
XM_005631247.2	XP_005631304.1	MAP kinase-activating death domain protein isoform X14
XM_014121189.1	XP_013976664.1	MAP kinase-activating death domain protein isoform X16
XM_855777.4	XP_860870.1	MAP kinase-activating death domain protein isoform X17
XM_005631249.2	XP_005631306.1	MAP kinase-activating death domain protein isoform X21
XM_014121182.2	XP_013976657.1	MAP kinase-activating death domain protein isoform X5
XM_005631243.3	XP_005631300.1	MAP kinase-activating death domain protein isoform X8
XM_014121185.2	XP_013976660.1	MAP kinase-activating death domain protein isoform X9
XM_533189.6	XP_533189.2	MAP kinase-activating death domain protein isoform X10
XM_855751.5	XP_860844.1	MAP kinase-activating death domain protein isoform X13
XM_855777.5	XP_860870.1	MAP kinase-activating death domain protein isoform X17
XM_005631249.3	XP_005631306.1	MAP kinase-activating death domain protein isoform X21
XM_014121181.1	XP_013976656.1	MAP kinase-activating death domain protein isoform X2
XM_005631246.2	XP_005631303.1	MAP kinase-activating death domain protein isoform X15
XM_014121190.1	XP_013976665.1	MAP kinase-activating death domain protein isoform X18
XM_014121182.1	XP_013976657.1	MAP kinase-activating death domain protein isoform X4
XM_014121186.1	XP_013976661.1	MAP kinase-activating death domain protein isoform X10
XM_014121186.2	XP_013976661.1	MAP kinase-activating death domain protein isoform X11
XM_005631246.3	XP_005631303.1	MAP kinase-activating death domain protein isoform X15
XM_855872.5	XP_860965.1	MAP kinase-activating death domain protein isoform X19
XM_014121180.1	XP_013976655.1	MAP kinase-activating death domain protein isoform X1
XM_014121183.1	XP_013976658.1	MAP kinase-activating death domain protein isoform X5
XM_005631243.2	XP_005631300.1	MAP kinase-activating death domain protein isoform X7
XM_014121187.1	XP_013976662.1	MAP kinase-activating death domain protein isoform X11
XM_855751.4	XP_860844.1	MAP kinase-activating death domain protein isoform X13
XM_014121183.2	XP_013976658.1	MAP kinase-activating death domain protein isoform X6
XM_014121184.2	XP_013976659.1	MAP kinase-activating death domain protein isoform X7
XM_014121190.2	XP_013976665.1	MAP kinase-activating death domain protein isoform X18
XM_005631250.2	XP_005631307.1	MAP kinase-activating death domain protein isoform X20
XM_014121177.1	XP_013976652.1	MAP kinase-activating death domain protein isoform X1
XM_014121184.1	XP_013976659.1	MAP kinase-activating death domain protein isoform X6
XM_014121185.1	XP_013976660.1	MAP kinase-activating death domain protein isoform X8
XM_855872.4	XP_860965.1	MAP kinase-activating death domain protein isoform X19
XM_014121177.2	XP_013976652.1	MAP kinase-activating death domain protein isoform X1
XM_014121178.2	XP_013976653.1	MAP kinase-activating death domain protein isoform X1
XM_014121180.2	XP_013976655.1	MAP kinase-activating death domain protein isoform X1
XM_014121179.2	XP_013976654.1	MAP kinase-activating death domain protein isoform X1
XM_014121188.2	XP_013976663.1	MAP kinase-activating death domain protein isoform X12
XM_014121189.2	XP_013976664.1	MAP kinase-activating death domain protein isoform X16
XM_014121191.2	XP_013976666.1	MAP kinase-activating death domain protein isoform X22

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MADD	NP_001180007.1
Rattus norvegicus (Norway rat)	Madd	NP_446037.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000630	XP_311160.5
Caenorhabditis elegans (roundworm)	aex-3	NP_741710.1
Xenopus tropicalis (tropical clawed frog)	madd	NP_001072717.1
Mus musculus (house mouse)	Madd	NP_001171191.1
Danio rerio (zebrafish)	madd	XP_005169012.1
	MADD	XP_001107689.1
Homo sapiens (human)	MADD	NP_003673.3
Pan troglodytes (chimpanzee)	MADD	XP_003951918.1
Canis lupus familiaris (dog)	MADD	XP_005631300.1
Gallus gallus (chicken)	MADD	XP_004941623.1
Drosophila melanogaster (fruit fly)	rab3-GEF	NP_001096983.2

Gene Ontology

The following MADD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MADD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe294792	XM_022405458.1 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41235	XM_014121177.2 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41235	XM_014121178.2 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41235	XM_014121179.2 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41235	XM_014121180.2 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41236	XM_014121181.2 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41237	XM_014121182.2 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41238	XM_014121183.2 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41239	XM_014121184.2 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41240	XM_014121185.2 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41241	XM_014121186.2 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41242	XM_014121187.1 Latest version!	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41243	XM_014121188.2 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41244	XM_014121189.2 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe20065	XM_005631241.3 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20066	XM_005631243.3 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20067	XM_533189.6 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20069	XM_855751.5 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20070	XM_005631246.3 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20071	XM_005631247.3 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20072	XM_855777.5 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20073	XM_014121190.2 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20074	XM_855872.5 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20075	XM_005631249.3 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20076	XM_005631250.3 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20077	XM_014121191.2 Latest version!	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OCe20065	XM_005631241.2	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20071	XM_005631247.2	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20067	XM_533189.5	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20072	XM_855777.4	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20075	XM_005631249.2	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe41236	XM_014121181.1	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41237	XM_014121182.1	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41241	XM_014121186.1	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe20070	XM_005631246.2	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20066	XM_005631243.2	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20069	XM_855751.4	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe41238	XM_014121183.1	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe20073	XM_014121190.1	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe20076	XM_005631250.2	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe41239	XM_014121184.1	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41240	XM_014121185.1	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe20074	XM_855872.4	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe41235	XM_014121177.1	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41235	XM_014121178.1	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41235	XM_014121180.1	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41235	XM_014121179.1	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41243	XM_014121188.1	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe41244	XM_014121189.1	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe20077	XM_014121191.1	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MADD ( XM_022405458.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022261166.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X6, mRNA.

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CloneID	OCe294792
Clone ID Related Accession (Same CDS sequence)	XM_022405458.1
Accession Version	XM_022405458.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTCTG GACTCACATG 
GCAGTAACTC AAACCTCGGC GCTCCAGAGG AGGGACTTAG AAGAGCCACC TCTGCAGAGA 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC 
TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT 
CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022261166.1
CDS	234..5186
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVWTHMAVTQTSALQRRDLEEPPLQRVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_022405458.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022405458.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTCTG GACTCACATG 
GCAGTAACTC AAACCTCGGC GCTCCAGAGG AGGGACTTAG AAGAGCCACC TCTGCAGAGA 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC 
TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT 
CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121178.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976653.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X2, mRNA.

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CloneID	OCe41235
Clone ID Related Accession (Same CDS sequence)	XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
Accession Version	XM_014121178.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976653.1
CDS	185..5140
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121178.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121178.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121179.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976654.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X3, mRNA.

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CloneID	OCe41235
Clone ID Related Accession (Same CDS sequence)	XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
Accession Version	XM_014121179.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976654.1
CDS	260..5215
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121179.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121179.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121180.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976655.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X4, mRNA.

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CloneID	OCe41235
Clone ID Related Accession (Same CDS sequence)	XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
Accession Version	XM_014121180.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976655.1
CDS	105..5060
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121180.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121180.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121177.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976652.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe41235
Clone ID Related Accession (Same CDS sequence)	XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
Accession Version	XM_014121177.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976652.1
CDS	254..5209
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121177.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121177.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121177.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976652.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe41235
Clone ID Related Accession (Same CDS sequence)	XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
Accession Version	XM_014121177.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976652.1
CDS	234..5189
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121177.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121177.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121178.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976653.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

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CloneID	OCe41235
Clone ID Related Accession (Same CDS sequence)	XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
Accession Version	XM_014121178.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121178.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976653.1
CDS	216..5171
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121178.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121178.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121180.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976655.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

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CloneID	OCe41235
Clone ID Related Accession (Same CDS sequence)	XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
Accession Version	XM_014121180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976655.1
CDS	206..5161
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121180.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121180.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121179.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976654.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X4, mRNA.

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CloneID	OCe41235
Clone ID Related Accession (Same CDS sequence)	XM_014121178.1 , XM_014121179.1 , XM_014121180.1 , XM_014121177.1 , XM_014121177.2 , XM_014121178.2 , XM_014121180.2 , XM_014121179.2
Accession Version	XM_014121179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121179.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976654.1
CDS	229..5184
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121179.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121179.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121181.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976656.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

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CloneID	OCe41236
Clone ID Related Accession (Same CDS sequence)	XM_014121181.2 , XM_014121181.1
Accession Version	XM_014121181.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA 
GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA 
ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG 
AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG 
GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG 
CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG 
TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT 
GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG 
GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC 
TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT 
CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976656.1
CDS	234..5186
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121181.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121181.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA 
GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA 
ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG 
AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG 
GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG 
CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG 
TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT 
GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG 
GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC 
TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT 
CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121181.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976656.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X5, mRNA.

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CloneID	OCe41236
Clone ID Related Accession (Same CDS sequence)	XM_014121181.2 , XM_014121181.1
Accession Version	XM_014121181.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA 
GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA 
ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG 
AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG 
GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG 
CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG 
TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT 
GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG 
GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC 
TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT 
CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976656.1
CDS	254..5206
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121181.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121181.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA 
GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA 
ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG 
AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG 
GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG 
CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG 
TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT 
GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG 
GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC 
TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT 
CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121182.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976657.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe41237
Clone ID Related Accession (Same CDS sequence)	XM_014121182.2 , XM_014121182.1
Accession Version	XM_014121182.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGTAAT 
AGTTCTGGAG AGACCCTTGG AGCAGATAGT GACCTGAGCA GCAATGCAGG TGATGGACCA 
GGTGGTGAAG GCAGCGCCCA CTTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT 
GAGACCAATT CTGCTACCAG CACCATCTTT GGGAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCCGAAG ATGTAAGCCA ACGGGTCTAT 
CTGTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGATGCG 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAA CGTTCTACTT TGTGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTGGGAG AACATGACCG GAAGCGCCTA 
GAGGATGATG AAGATCGTTT GCTGGCCACA CTTTTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTAAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG CCAGCAAATC AATGAAGTGC TTGATCAGCT GGTGAACCTG 
AATGGACGTG ATCTCTCTAT CCGGTCCAGT GGCAGCCGGC ACATGAAAAA GCAGACATTT 
GTGGTACATG CAGGGACAGA CACAAATGGA GACATCTTCT TCATGGAGGT GTGTGATGAC 
TGTGTGGTGT TACGTAGTAA CATCGGAACG GTGTATGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACCAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACTCAGC TCAACAAATT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGGGCTGC TGCCCGGCAG CAAAGCATCA AACCTGGCCC TGAACTGGGT 
GGCGAGTTCC CTGTGCAGGA CATGAAGACT GGTGAGGGTG GCTTGCTGCA GGTCACCCTA 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCATGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCTGCA GTTCGTAGCA GTGAGGAAGA TCTCAGGACC 
CCACCCCGGC CCGTCTCGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976657.1
CDS	234..5177
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121182.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121182.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGTAAT 
AGTTCTGGAG AGACCCTTGG AGCAGATAGT GACCTGAGCA GCAATGCAGG TGATGGACCA 
GGTGGTGAAG GCAGCGCCCA CTTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT 
GAGACCAATT CTGCTACCAG CACCATCTTT GGGAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCCGAAG ATGTAAGCCA ACGGGTCTAT 
CTGTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGATGCG 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAA CGTTCTACTT TGTGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTGGGAG AACATGACCG GAAGCGCCTA 
GAGGATGATG AAGATCGTTT GCTGGCCACA CTTTTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTAAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG CCAGCAAATC AATGAAGTGC TTGATCAGCT GGTGAACCTG 
AATGGACGTG ATCTCTCTAT CCGGTCCAGT GGCAGCCGGC ACATGAAAAA GCAGACATTT 
GTGGTACATG CAGGGACAGA CACAAATGGA GACATCTTCT TCATGGAGGT GTGTGATGAC 
TGTGTGGTGT TACGTAGTAA CATCGGAACG GTGTATGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACCAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACTCAGC TCAACAAATT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGGGCTGC TGCCCGGCAG CAAAGCATCA AACCTGGCCC TGAACTGGGT 
GGCGAGTTCC CTGTGCAGGA CATGAAGACT GGTGAGGGTG GCTTGCTGCA GGTCACCCTA 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCATGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCTGCA GTTCGTAGCA GTGAGGAAGA TCTCAGGACC 
CCACCCCGGC CCGTCTCGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121182.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976657.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X7, mRNA.

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CloneID	OCe41237
Clone ID Related Accession (Same CDS sequence)	XM_014121182.2 , XM_014121182.1
Accession Version	XM_014121182.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGTAAT 
AGTTCTGGAG AGACCCTTGG AGCAGATAGT GACCTGAGCA GCAATGCAGG TGATGGACCA 
GGTGGTGAAG GCAGCGCCCA CTTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT 
GAGACCAATT CTGCTACCAG CACCATCTTT GGGAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCCGAAG ATGTAAGCCA ACGGGTCTAT 
CTGTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGATGCG 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAA CGTTCTACTT TGTGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTGGGAG AACATGACCG GAAGCGCCTA 
GAGGATGATG AAGATCGTTT GCTGGCCACA CTTTTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTAAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG CCAGCAAATC AATGAAGTGC TTGATCAGCT GGTGAACCTG 
AATGGACGTG ATCTCTCTAT CCGGTCCAGT GGCAGCCGGC ACATGAAAAA GCAGACATTT 
GTGGTACATG CAGGGACAGA CACAAATGGA GACATCTTCT TCATGGAGGT GTGTGATGAC 
TGTGTGGTGT TACGTAGTAA CATCGGAACG GTGTATGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACCAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACTCAGC TCAACAAATT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGGGCTGC TGCCCGGCAG CAAAGCATCA AACCTGGCCC TGAACTGGGT 
GGCGAGTTCC CTGTGCAGGA CATGAAGACT GGTGAGGGTG GCTTGCTGCA GGTCACCCTA 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCATGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCTGCA GTTCGTAGCA GTGAGGAAGA TCTCAGGACC 
CCACCCCGGC CCGTCTCGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976657.1
CDS	254..5197
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121182.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121182.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTGAGTAAT 
AGTTCTGGAG AGACCCTTGG AGCAGATAGT GACCTGAGCA GCAATGCAGG TGATGGACCA 
GGTGGTGAAG GCAGCGCCCA CTTGGCAAGC TCTCGGGGCA CTTTGTCTGA TAGTGAAATT 
GAGACCAATT CTGCTACCAG CACCATCTTT GGGAAAGCCC ATAGCTTGAA GCCAAGTGTA 
AAGGAGAAGC TGGTGGGCAG CCCAGTTCGC TCTTCCGAAG ATGTAAGCCA ACGGGTCTAT 
CTGTACGAGG GACTCCTAGG AAGGGACAAA GGATCGATGT GGGACCAGTT AGAGGATGCG 
GCTATGGAGA CCTTTTCTAT AAGCAAAGAA CGTTCTACTT TGTGGGACCA AATGCAGTTC 
TGGGAAGATG CATTCTTAGA TGCTGTGATG TTGGAGAGAG AAGGGATGGG TATGGACCAG 
GGTCCCCAGG AAATGATCGA CAGGTACCTG TCCCTGGGAG AACATGACCG GAAGCGCCTA 
GAGGATGATG AAGATCGTTT GCTGGCCACA CTTTTGCACA ACCTCATCTC CTACATGCTG 
CTGATGAAGG TAAATAAGAA TGACATCCGG AAGAAGGTGA GGCGCCTAAT GGGAAAGTCC 
CATATTGGGC TTGTGTACAG CCAGCAAATC AATGAAGTGC TTGATCAGCT GGTGAACCTG 
AATGGACGTG ATCTCTCTAT CCGGTCCAGT GGCAGCCGGC ACATGAAAAA GCAGACATTT 
GTGGTACATG CAGGGACAGA CACAAATGGA GACATCTTCT TCATGGAGGT GTGTGATGAC 
TGTGTGGTGT TACGTAGTAA CATCGGAACG GTGTATGAGC GCTGGTGGTA TGAGAAGCTC 
ATCAACATGA CCTACTGTCC CAAGACCAAG GTGTTGTGCT TGTGGCGTAG AAATGGCTCT 
GAGACTCAGC TCAACAAATT CTATACTAAG AAGTGTCGGG AGCTGTACTA CTGTGTGAAG 
GACAGCATGG AGCGGGCTGC TGCCCGGCAG CAAAGCATCA AACCTGGCCC TGAACTGGGT 
GGCGAGTTCC CTGTGCAGGA CATGAAGACT GGTGAGGGTG GCTTGCTGCA GGTCACCCTA 
GAAGGGATCA ATCTCAAGTT CATGCACAAC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT 
CACATTAAAA AGTGCAATAC AGTCCGAGGC GTCTTTGTCC TGGAGGAATT TGTTCCTGAA 
ATTAAAGAAG TGGTGAGCCA CAAGTACAAG ACACCAATGG CCCATGAGAT CTGCTACTCT 
GTGTTGTGTC TCTTCTCGTA TGTGGCTGCA GTTCGTAGCA GTGAGGAAGA TCTCAGGACC 
CCACCCCGGC CCGTCTCGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121183.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976658.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X8, mRNA.

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CloneID	OCe41238
Clone ID Related Accession (Same CDS sequence)	XM_014121183.1 , XM_014121183.2
Accession Version	XM_014121183.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGCTTGAA ACTGGCAAGC GACTCAGACG CAGAGTCAGA CTCTCGAGCG 
AGCTCTCCCA CCTCCACCGT CTCCAACACC AGCACCGAGG GCTTCGGGGG CATCATGTCT 
TTTGCCAGCA GCCTGTATCG AAACCACAGT ACAAGCTTCA GTCTTTCAAA CCTCACACTG 
CCGACCAAAG GTGCCCGAGA GAAGACGACC CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA 
AATACTCTAA TGGAGATTGT TACTGAAGCC GGCCCCGGGA GTGGTGAAGG AAACAGGAGG 
GCCTTAGTGG ACCAGAAGTC ATCTGTTATT AAACACAGCC CAACAGTGAA AAGAGAGCCT 
CCATCACCTC AGGGTCGATC CAGCAATTCT AGTGAGAACC AGCAGTTCCT GAAGGAAGTG 
GTACACAGCG TGCTGGATGG CCAGGGTGTT GGCTGGCTCA ACATGAAAAA GGTGCGTCGG 
CTGCTGGAGA GTGAGCAGCT GCGAGTCTTT GTCCTGAGCA AGCTGAACCG AACTGTGCAG 
TCAGAAGATG ATGCCCGGCA GGACGTCATC CCCGATGTGG AGATCAGCAG AAAGGTTTAC 
AAGGGGATGT TAGACCTGCT CAAGTGCACA GTACTCAGCC TGGAGCAGTC CTATGCCCAC 
GCAGGTCTGG GTGGCATGGC CAGCATCTTT GGGCTTCTGG AGATTGCCCA GACCCACTAC 
TATAGTAAAG AACCAGACAA GCGGAAGAGA AGTCCAACAG AGAGTGTAAA TACACCAGTT 
GGCAAGGATC CTGGCCTGGC TGGGCGGGGG GACCCAAAAG CTATGGCACA GCTGAGAGTT 
CCCCAGCTGG GCCCTCGGGC GCCAAGTGCT GCAGGAAAGG GTCCCAAAGA ACTAGACACC 
AGAAGTTTAA AGGAAGAGAA TTTTGTAGCA TCTGTTGAAT TGTGGAACAA GCACCAGGAA 
GTGAAAAAGC AAAAAGCTTT GGAAAAACAG AGGCCTGAAG TCATCAAACC CGTCTTTGAC 
CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG ATCAGCGCAG ACAGTGGTGT GAGCCTGACA 
TCTGGTTCCC AGAGGACTGA TACAGACTCT GTCATTGGTG TGAGTCCAGC TGTTATGATC 
CGAAGCTCAA GTCAGGACTC TGAAGTTAGC ACCGTGGTGA GTAATAGTTC TGGAGAGACC 
CTTGGAGCAG ATAGTGACCT GAGCAGCAAT GCAGGTGATG GACCAGGTGG TGAAGGCAGC 
GCCCACTTGG CAAGCTCTCG GGGCACTTTG TCTGATAGTG AAATTGAGAC CAATTCTGCT 
ACCAGCACCA TCTTTGGGAA AGCCCATAGC TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG 
GGCAGCCCAG TTCGCTCTTC CGAAGATGTA AGCCAACGGG TCTATCTGTA CGAGGGACTC 
CTAGGAAGGG ACAAAGGATC GATGTGGGAC CAGTTAGAGG ATGCGGCTAT GGAGACCTTT 
TCTATAAGCA AAGAACGTTC TACTTTGTGG GACCAAATGC AGTTCTGGGA AGATGCATTC 
TTAGATGCTG TGATGTTGGA GAGAGAAGGG ATGGGTATGG ACCAGGGTCC CCAGGAAATG 
ATCGACAGGT ACCTGTCCCT GGGAGAACAT GACCGGAAGC GCCTAGAGGA TGATGAAGAT 
CGTTTGCTGG CCACACTTTT GCACAACCTC ATCTCCTACA TGCTGCTGAT GAAGGTAAAT 
AAGAATGACA TCCGGAAGAA GGTGAGGCGC CTAATGGGAA AGTCCCATAT TGGGCTTGTG 
TACAGCCAGC AAATCAATGA AGTGCTTGAT CAGCTGGTGA ACCTGAATGG ACGTGATCTC 
TCTATCCGGT CCAGTGGCAG CCGGCACATG AAAAAGCAGA CATTTGTGGT ACATGCAGGG 
ACAGACACAA ATGGAGACAT CTTCTTCATG GAGGTGTGTG ATGACTGTGT GGTGTTACGT 
AGTAACATCG GAACGGTGTA TGAGCGCTGG TGGTATGAGA AGCTCATCAA CATGACCTAC 
TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG CGTAGAAATG GCTCTGAGAC TCAGCTCAAC 
AAATTCTATA CTAAGAAGTG TCGGGAGCTG TACTACTGTG TGAAGGACAG CATGGAGCGG 
GCTGCTGCCC GGCAGCAAAG CATCAAACCT GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG 
CAGGACATGA AGACTGGTGA GGGTGGCTTG CTGCAGGTCA CCCTAGAAGG GATCAATCTC 
AAGTTCATGC ACAACCAGGA GCGGAAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC 
TCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976658.1
CDS	255..5183
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121183.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121183.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGCTTGAA ACTGGCAAGC GACTCAGACG CAGAGTCAGA CTCTCGAGCG 
AGCTCTCCCA CCTCCACCGT CTCCAACACC AGCACCGAGG GCTTCGGGGG CATCATGTCT 
TTTGCCAGCA GCCTGTATCG AAACCACAGT ACAAGCTTCA GTCTTTCAAA CCTCACACTG 
CCGACCAAAG GTGCCCGAGA GAAGACGACC CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA 
AATACTCTAA TGGAGATTGT TACTGAAGCC GGCCCCGGGA GTGGTGAAGG AAACAGGAGG 
GCCTTAGTGG ACCAGAAGTC ATCTGTTATT AAACACAGCC CAACAGTGAA AAGAGAGCCT 
CCATCACCTC AGGGTCGATC CAGCAATTCT AGTGAGAACC AGCAGTTCCT GAAGGAAGTG 
GTACACAGCG TGCTGGATGG CCAGGGTGTT GGCTGGCTCA ACATGAAAAA GGTGCGTCGG 
CTGCTGGAGA GTGAGCAGCT GCGAGTCTTT GTCCTGAGCA AGCTGAACCG AACTGTGCAG 
TCAGAAGATG ATGCCCGGCA GGACGTCATC CCCGATGTGG AGATCAGCAG AAAGGTTTAC 
AAGGGGATGT TAGACCTGCT CAAGTGCACA GTACTCAGCC TGGAGCAGTC CTATGCCCAC 
GCAGGTCTGG GTGGCATGGC CAGCATCTTT GGGCTTCTGG AGATTGCCCA GACCCACTAC 
TATAGTAAAG AACCAGACAA GCGGAAGAGA AGTCCAACAG AGAGTGTAAA TACACCAGTT 
GGCAAGGATC CTGGCCTGGC TGGGCGGGGG GACCCAAAAG CTATGGCACA GCTGAGAGTT 
CCCCAGCTGG GCCCTCGGGC GCCAAGTGCT GCAGGAAAGG GTCCCAAAGA ACTAGACACC 
AGAAGTTTAA AGGAAGAGAA TTTTGTAGCA TCTGTTGAAT TGTGGAACAA GCACCAGGAA 
GTGAAAAAGC AAAAAGCTTT GGAAAAACAG AGGCCTGAAG TCATCAAACC CGTCTTTGAC 
CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG ATCAGCGCAG ACAGTGGTGT GAGCCTGACA 
TCTGGTTCCC AGAGGACTGA TACAGACTCT GTCATTGGTG TGAGTCCAGC TGTTATGATC 
CGAAGCTCAA GTCAGGACTC TGAAGTTAGC ACCGTGGTGA GTAATAGTTC TGGAGAGACC 
CTTGGAGCAG ATAGTGACCT GAGCAGCAAT GCAGGTGATG GACCAGGTGG TGAAGGCAGC 
GCCCACTTGG CAAGCTCTCG GGGCACTTTG TCTGATAGTG AAATTGAGAC CAATTCTGCT 
ACCAGCACCA TCTTTGGGAA AGCCCATAGC TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG 
GGCAGCCCAG TTCGCTCTTC CGAAGATGTA AGCCAACGGG TCTATCTGTA CGAGGGACTC 
CTAGGAAGGG ACAAAGGATC GATGTGGGAC CAGTTAGAGG ATGCGGCTAT GGAGACCTTT 
TCTATAAGCA AAGAACGTTC TACTTTGTGG GACCAAATGC AGTTCTGGGA AGATGCATTC 
TTAGATGCTG TGATGTTGGA GAGAGAAGGG ATGGGTATGG ACCAGGGTCC CCAGGAAATG 
ATCGACAGGT ACCTGTCCCT GGGAGAACAT GACCGGAAGC GCCTAGAGGA TGATGAAGAT 
CGTTTGCTGG CCACACTTTT GCACAACCTC ATCTCCTACA TGCTGCTGAT GAAGGTAAAT 
AAGAATGACA TCCGGAAGAA GGTGAGGCGC CTAATGGGAA AGTCCCATAT TGGGCTTGTG 
TACAGCCAGC AAATCAATGA AGTGCTTGAT CAGCTGGTGA ACCTGAATGG ACGTGATCTC 
TCTATCCGGT CCAGTGGCAG CCGGCACATG AAAAAGCAGA CATTTGTGGT ACATGCAGGG 
ACAGACACAA ATGGAGACAT CTTCTTCATG GAGGTGTGTG ATGACTGTGT GGTGTTACGT 
AGTAACATCG GAACGGTGTA TGAGCGCTGG TGGTATGAGA AGCTCATCAA CATGACCTAC 
TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG CGTAGAAATG GCTCTGAGAC TCAGCTCAAC 
AAATTCTATA CTAAGAAGTG TCGGGAGCTG TACTACTGTG TGAAGGACAG CATGGAGCGG 
GCTGCTGCCC GGCAGCAAAG CATCAAACCT GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG 
CAGGACATGA AGACTGGTGA GGGTGGCTTG CTGCAGGTCA CCCTAGAAGG GATCAATCTC 
AAGTTCATGC ACAACCAGGA GCGGAAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC 
TCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121183.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976658.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

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CloneID	OCe41238
Clone ID Related Accession (Same CDS sequence)	XM_014121183.1 , XM_014121183.2
Accession Version	XM_014121183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGCTTGAA ACTGGCAAGC GACTCAGACG CAGAGTCAGA CTCTCGAGCG 
AGCTCTCCCA CCTCCACCGT CTCCAACACC AGCACCGAGG GCTTCGGGGG CATCATGTCT 
TTTGCCAGCA GCCTGTATCG AAACCACAGT ACAAGCTTCA GTCTTTCAAA CCTCACACTG 
CCGACCAAAG GTGCCCGAGA GAAGACGACC CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA 
AATACTCTAA TGGAGATTGT TACTGAAGCC GGCCCCGGGA GTGGTGAAGG AAACAGGAGG 
GCCTTAGTGG ACCAGAAGTC ATCTGTTATT AAACACAGCC CAACAGTGAA AAGAGAGCCT 
CCATCACCTC AGGGTCGATC CAGCAATTCT AGTGAGAACC AGCAGTTCCT GAAGGAAGTG 
GTACACAGCG TGCTGGATGG CCAGGGTGTT GGCTGGCTCA ACATGAAAAA GGTGCGTCGG 
CTGCTGGAGA GTGAGCAGCT GCGAGTCTTT GTCCTGAGCA AGCTGAACCG AACTGTGCAG 
TCAGAAGATG ATGCCCGGCA GGACGTCATC CCCGATGTGG AGATCAGCAG AAAGGTTTAC 
AAGGGGATGT TAGACCTGCT CAAGTGCACA GTACTCAGCC TGGAGCAGTC CTATGCCCAC 
GCAGGTCTGG GTGGCATGGC CAGCATCTTT GGGCTTCTGG AGATTGCCCA GACCCACTAC 
TATAGTAAAG AACCAGACAA GCGGAAGAGA AGTCCAACAG AGAGTGTAAA TACACCAGTT 
GGCAAGGATC CTGGCCTGGC TGGGCGGGGG GACCCAAAAG CTATGGCACA GCTGAGAGTT 
CCCCAGCTGG GCCCTCGGGC GCCAAGTGCT GCAGGAAAGG GTCCCAAAGA ACTAGACACC 
AGAAGTTTAA AGGAAGAGAA TTTTGTAGCA TCTGTTGAAT TGTGGAACAA GCACCAGGAA 
GTGAAAAAGC AAAAAGCTTT GGAAAAACAG AGGCCTGAAG TCATCAAACC CGTCTTTGAC 
CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG ATCAGCGCAG ACAGTGGTGT GAGCCTGACA 
TCTGGTTCCC AGAGGACTGA TACAGACTCT GTCATTGGTG TGAGTCCAGC TGTTATGATC 
CGAAGCTCAA GTCAGGACTC TGAAGTTAGC ACCGTGGTGA GTAATAGTTC TGGAGAGACC 
CTTGGAGCAG ATAGTGACCT GAGCAGCAAT GCAGGTGATG GACCAGGTGG TGAAGGCAGC 
GCCCACTTGG CAAGCTCTCG GGGCACTTTG TCTGATAGTG AAATTGAGAC CAATTCTGCT 
ACCAGCACCA TCTTTGGGAA AGCCCATAGC TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG 
GGCAGCCCAG TTCGCTCTTC CGAAGATGTA AGCCAACGGG TCTATCTGTA CGAGGGACTC 
CTAGGAAGGG ACAAAGGATC GATGTGGGAC CAGTTAGAGG ATGCGGCTAT GGAGACCTTT 
TCTATAAGCA AAGAACGTTC TACTTTGTGG GACCAAATGC AGTTCTGGGA AGATGCATTC 
TTAGATGCTG TGATGTTGGA GAGAGAAGGG ATGGGTATGG ACCAGGGTCC CCAGGAAATG 
ATCGACAGGT ACCTGTCCCT GGGAGAACAT GACCGGAAGC GCCTAGAGGA TGATGAAGAT 
CGTTTGCTGG CCACACTTTT GCACAACCTC ATCTCCTACA TGCTGCTGAT GAAGGTAAAT 
AAGAATGACA TCCGGAAGAA GGTGAGGCGC CTAATGGGAA AGTCCCATAT TGGGCTTGTG 
TACAGCCAGC AAATCAATGA AGTGCTTGAT CAGCTGGTGA ACCTGAATGG ACGTGATCTC 
TCTATCCGGT CCAGTGGCAG CCGGCACATG AAAAAGCAGA CATTTGTGGT ACATGCAGGG 
ACAGACACAA ATGGAGACAT CTTCTTCATG GAGGTGTGTG ATGACTGTGT GGTGTTACGT 
AGTAACATCG GAACGGTGTA TGAGCGCTGG TGGTATGAGA AGCTCATCAA CATGACCTAC 
TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG CGTAGAAATG GCTCTGAGAC TCAGCTCAAC 
AAATTCTATA CTAAGAAGTG TCGGGAGCTG TACTACTGTG TGAAGGACAG CATGGAGCGG 
GCTGCTGCCC GGCAGCAAAG CATCAAACCT GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG 
CAGGACATGA AGACTGGTGA GGGTGGCTTG CTGCAGGTCA CCCTAGAAGG GATCAATCTC 
AAGTTCATGC ACAACCAGGA GCGGAAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC 
TCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976658.1
CDS	234..5162
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121183.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121183.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGCTTGAA ACTGGCAAGC GACTCAGACG CAGAGTCAGA CTCTCGAGCG 
AGCTCTCCCA CCTCCACCGT CTCCAACACC AGCACCGAGG GCTTCGGGGG CATCATGTCT 
TTTGCCAGCA GCCTGTATCG AAACCACAGT ACAAGCTTCA GTCTTTCAAA CCTCACACTG 
CCGACCAAAG GTGCCCGAGA GAAGACGACC CCCTTCCCCA GTCTGAAAGT ATTTGGGCTA 
AATACTCTAA TGGAGATTGT TACTGAAGCC GGCCCCGGGA GTGGTGAAGG AAACAGGAGG 
GCCTTAGTGG ACCAGAAGTC ATCTGTTATT AAACACAGCC CAACAGTGAA AAGAGAGCCT 
CCATCACCTC AGGGTCGATC CAGCAATTCT AGTGAGAACC AGCAGTTCCT GAAGGAAGTG 
GTACACAGCG TGCTGGATGG CCAGGGTGTT GGCTGGCTCA ACATGAAAAA GGTGCGTCGG 
CTGCTGGAGA GTGAGCAGCT GCGAGTCTTT GTCCTGAGCA AGCTGAACCG AACTGTGCAG 
TCAGAAGATG ATGCCCGGCA GGACGTCATC CCCGATGTGG AGATCAGCAG AAAGGTTTAC 
AAGGGGATGT TAGACCTGCT CAAGTGCACA GTACTCAGCC TGGAGCAGTC CTATGCCCAC 
GCAGGTCTGG GTGGCATGGC CAGCATCTTT GGGCTTCTGG AGATTGCCCA GACCCACTAC 
TATAGTAAAG AACCAGACAA GCGGAAGAGA AGTCCAACAG AGAGTGTAAA TACACCAGTT 
GGCAAGGATC CTGGCCTGGC TGGGCGGGGG GACCCAAAAG CTATGGCACA GCTGAGAGTT 
CCCCAGCTGG GCCCTCGGGC GCCAAGTGCT GCAGGAAAGG GTCCCAAAGA ACTAGACACC 
AGAAGTTTAA AGGAAGAGAA TTTTGTAGCA TCTGTTGAAT TGTGGAACAA GCACCAGGAA 
GTGAAAAAGC AAAAAGCTTT GGAAAAACAG AGGCCTGAAG TCATCAAACC CGTCTTTGAC 
CTTGGTGAGA CAGAGGAGAA AAAGTCCCAG ATCAGCGCAG ACAGTGGTGT GAGCCTGACA 
TCTGGTTCCC AGAGGACTGA TACAGACTCT GTCATTGGTG TGAGTCCAGC TGTTATGATC 
CGAAGCTCAA GTCAGGACTC TGAAGTTAGC ACCGTGGTGA GTAATAGTTC TGGAGAGACC 
CTTGGAGCAG ATAGTGACCT GAGCAGCAAT GCAGGTGATG GACCAGGTGG TGAAGGCAGC 
GCCCACTTGG CAAGCTCTCG GGGCACTTTG TCTGATAGTG AAATTGAGAC CAATTCTGCT 
ACCAGCACCA TCTTTGGGAA AGCCCATAGC TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG 
GGCAGCCCAG TTCGCTCTTC CGAAGATGTA AGCCAACGGG TCTATCTGTA CGAGGGACTC 
CTAGGAAGGG ACAAAGGATC GATGTGGGAC CAGTTAGAGG ATGCGGCTAT GGAGACCTTT 
TCTATAAGCA AAGAACGTTC TACTTTGTGG GACCAAATGC AGTTCTGGGA AGATGCATTC 
TTAGATGCTG TGATGTTGGA GAGAGAAGGG ATGGGTATGG ACCAGGGTCC CCAGGAAATG 
ATCGACAGGT ACCTGTCCCT GGGAGAACAT GACCGGAAGC GCCTAGAGGA TGATGAAGAT 
CGTTTGCTGG CCACACTTTT GCACAACCTC ATCTCCTACA TGCTGCTGAT GAAGGTAAAT 
AAGAATGACA TCCGGAAGAA GGTGAGGCGC CTAATGGGAA AGTCCCATAT TGGGCTTGTG 
TACAGCCAGC AAATCAATGA AGTGCTTGAT CAGCTGGTGA ACCTGAATGG ACGTGATCTC 
TCTATCCGGT CCAGTGGCAG CCGGCACATG AAAAAGCAGA CATTTGTGGT ACATGCAGGG 
ACAGACACAA ATGGAGACAT CTTCTTCATG GAGGTGTGTG ATGACTGTGT GGTGTTACGT 
AGTAACATCG GAACGGTGTA TGAGCGCTGG TGGTATGAGA AGCTCATCAA CATGACCTAC 
TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG CGTAGAAATG GCTCTGAGAC TCAGCTCAAC 
AAATTCTATA CTAAGAAGTG TCGGGAGCTG TACTACTGTG TGAAGGACAG CATGGAGCGG 
GCTGCTGCCC GGCAGCAAAG CATCAAACCT GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG 
CAGGACATGA AGACTGGTGA GGGTGGCTTG CTGCAGGTCA CCCTAGAAGG GATCAATCTC 
AAGTTCATGC ACAACCAGGA GCGGAAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC 
TCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121184.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976659.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

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CloneID	OCe41239
Clone ID Related Accession (Same CDS sequence)	XM_014121184.2 , XM_014121184.1
Accession Version	XM_014121184.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121184.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC 
AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT 
TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT 
AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT 
GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGAA GGGACAAAGG ATCGATGTGG 
GACCAGTTAG AGGATGCGGC TATGGAGACC TTTTCTATAA GCAAAGAACG TTCTACTTTG 
TGGGACCAAA TGCAGTTCTG GGAAGATGCA TTCTTAGATG CTGTGATGTT GGAGAGAGAA 
GGGATGGGTA TGGACCAGGG TCCCCAGGAA ATGATCGACA GGTACCTGTC CCTGGGAGAA 
CATGACCGGA AGCGCCTAGA GGATGATGAA GATCGTTTGC TGGCCACACT TTTGCACAAC 
CTCATCTCCT ACATGCTGCT GATGAAGGTA AATAAGAATG ACATCCGGAA GAAGGTGAGG 
CGCCTAATGG GAAAGTCCCA TATTGGGCTT GTGTACAGCC AGCAAATCAA TGAAGTGCTT 
GATCAGCTGG TGAACCTGAA TGGACGTGAT CTCTCTATCC GGTCCAGTGG CAGCCGGCAC 
ATGAAAAAGC AGACATTTGT GGTACATGCA GGGACAGACA CAAATGGAGA CATCTTCTTC 
ATGGAGGTGT GTGATGACTG TGTGGTGTTA CGTAGTAACA TCGGAACGGT GTATGAGCGC 
TGGTGGTATG AGAAGCTCAT CAACATGACC TACTGTCCCA AGACCAAGGT GTTGTGCTTG 
TGGCGTAGAA ATGGCTCTGA GACTCAGCTC AACAAATTCT ATACTAAGAA GTGTCGGGAG 
CTGTACTACT GTGTGAAGGA CAGCATGGAG CGGGCTGCTG CCCGGCAGCA AAGCATCAAA 
CCTGGCCCTG AACTGGGTGG CGAGTTCCCT GTGCAGGACA TGAAGACTGG TGAGGGTGGC 
TTGCTGCAGG TCACCCTAGA AGGGATCAAT CTCAAGTTCA TGCACAACCA GGAGCGGAAG 
GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TCCGAGGCGT CTTTGTCCTG 
GAGGAATTTG TTCCTGAAAT TAAAGAAGTG GTGAGCCACA AGTACAAGAC ACCAATGGCC 
CATGAGATCT GCTACTCTGT GTTGTGTCTC TTCTCGTATG TGGCTGCAGT TCGTAGCAGT 
GAGGAAGATC TCAGGACCCC ACCCCGGCCC GTCTCGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976659.1
CDS	234..5135
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121184.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121184.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC 
AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT 
TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT 
AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT 
GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGAA GGGACAAAGG ATCGATGTGG 
GACCAGTTAG AGGATGCGGC TATGGAGACC TTTTCTATAA GCAAAGAACG TTCTACTTTG 
TGGGACCAAA TGCAGTTCTG GGAAGATGCA TTCTTAGATG CTGTGATGTT GGAGAGAGAA 
GGGATGGGTA TGGACCAGGG TCCCCAGGAA ATGATCGACA GGTACCTGTC CCTGGGAGAA 
CATGACCGGA AGCGCCTAGA GGATGATGAA GATCGTTTGC TGGCCACACT TTTGCACAAC 
CTCATCTCCT ACATGCTGCT GATGAAGGTA AATAAGAATG ACATCCGGAA GAAGGTGAGG 
CGCCTAATGG GAAAGTCCCA TATTGGGCTT GTGTACAGCC AGCAAATCAA TGAAGTGCTT 
GATCAGCTGG TGAACCTGAA TGGACGTGAT CTCTCTATCC GGTCCAGTGG CAGCCGGCAC 
ATGAAAAAGC AGACATTTGT GGTACATGCA GGGACAGACA CAAATGGAGA CATCTTCTTC 
ATGGAGGTGT GTGATGACTG TGTGGTGTTA CGTAGTAACA TCGGAACGGT GTATGAGCGC 
TGGTGGTATG AGAAGCTCAT CAACATGACC TACTGTCCCA AGACCAAGGT GTTGTGCTTG 
TGGCGTAGAA ATGGCTCTGA GACTCAGCTC AACAAATTCT ATACTAAGAA GTGTCGGGAG 
CTGTACTACT GTGTGAAGGA CAGCATGGAG CGGGCTGCTG CCCGGCAGCA AAGCATCAAA 
CCTGGCCCTG AACTGGGTGG CGAGTTCCCT GTGCAGGACA TGAAGACTGG TGAGGGTGGC 
TTGCTGCAGG TCACCCTAGA AGGGATCAAT CTCAAGTTCA TGCACAACCA GGAGCGGAAG 
GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TCCGAGGCGT CTTTGTCCTG 
GAGGAATTTG TTCCTGAAAT TAAAGAAGTG GTGAGCCACA AGTACAAGAC ACCAATGGCC 
CATGAGATCT GCTACTCTGT GTTGTGTCTC TTCTCGTATG TGGCTGCAGT TCGTAGCAGT 
GAGGAAGATC TCAGGACCCC ACCCCGGCCC GTCTCGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121184.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976659.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X9, mRNA.

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CloneID	OCe41239
Clone ID Related Accession (Same CDS sequence)	XM_014121184.2 , XM_014121184.1
Accession Version	XM_014121184.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC 
AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT 
TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT 
AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT 
GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGAA GGGACAAAGG ATCGATGTGG 
GACCAGTTAG AGGATGCGGC TATGGAGACC TTTTCTATAA GCAAAGAACG TTCTACTTTG 
TGGGACCAAA TGCAGTTCTG GGAAGATGCA TTCTTAGATG CTGTGATGTT GGAGAGAGAA 
GGGATGGGTA TGGACCAGGG TCCCCAGGAA ATGATCGACA GGTACCTGTC CCTGGGAGAA 
CATGACCGGA AGCGCCTAGA GGATGATGAA GATCGTTTGC TGGCCACACT TTTGCACAAC 
CTCATCTCCT ACATGCTGCT GATGAAGGTA AATAAGAATG ACATCCGGAA GAAGGTGAGG 
CGCCTAATGG GAAAGTCCCA TATTGGGCTT GTGTACAGCC AGCAAATCAA TGAAGTGCTT 
GATCAGCTGG TGAACCTGAA TGGACGTGAT CTCTCTATCC GGTCCAGTGG CAGCCGGCAC 
ATGAAAAAGC AGACATTTGT GGTACATGCA GGGACAGACA CAAATGGAGA CATCTTCTTC 
ATGGAGGTGT GTGATGACTG TGTGGTGTTA CGTAGTAACA TCGGAACGGT GTATGAGCGC 
TGGTGGTATG AGAAGCTCAT CAACATGACC TACTGTCCCA AGACCAAGGT GTTGTGCTTG 
TGGCGTAGAA ATGGCTCTGA GACTCAGCTC AACAAATTCT ATACTAAGAA GTGTCGGGAG 
CTGTACTACT GTGTGAAGGA CAGCATGGAG CGGGCTGCTG CCCGGCAGCA AAGCATCAAA 
CCTGGCCCTG AACTGGGTGG CGAGTTCCCT GTGCAGGACA TGAAGACTGG TGAGGGTGGC 
TTGCTGCAGG TCACCCTAGA AGGGATCAAT CTCAAGTTCA TGCACAACCA GGAGCGGAAG 
GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TCCGAGGCGT CTTTGTCCTG 
GAGGAATTTG TTCCTGAAAT TAAAGAAGTG GTGAGCCACA AGTACAAGAC ACCAATGGCC 
CATGAGATCT GCTACTCTGT GTTGTGTCTC TTCTCGTATG TGGCTGCAGT TCGTAGCAGT 
GAGGAAGATC TCAGGACCCC ACCCCGGCCC GTCTCGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976659.1
CDS	254..5155
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121184.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121184.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC 
AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT 
TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT 
AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT 
GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGAA GGGACAAAGG ATCGATGTGG 
GACCAGTTAG AGGATGCGGC TATGGAGACC TTTTCTATAA GCAAAGAACG TTCTACTTTG 
TGGGACCAAA TGCAGTTCTG GGAAGATGCA TTCTTAGATG CTGTGATGTT GGAGAGAGAA 
GGGATGGGTA TGGACCAGGG TCCCCAGGAA ATGATCGACA GGTACCTGTC CCTGGGAGAA 
CATGACCGGA AGCGCCTAGA GGATGATGAA GATCGTTTGC TGGCCACACT TTTGCACAAC 
CTCATCTCCT ACATGCTGCT GATGAAGGTA AATAAGAATG ACATCCGGAA GAAGGTGAGG 
CGCCTAATGG GAAAGTCCCA TATTGGGCTT GTGTACAGCC AGCAAATCAA TGAAGTGCTT 
GATCAGCTGG TGAACCTGAA TGGACGTGAT CTCTCTATCC GGTCCAGTGG CAGCCGGCAC 
ATGAAAAAGC AGACATTTGT GGTACATGCA GGGACAGACA CAAATGGAGA CATCTTCTTC 
ATGGAGGTGT GTGATGACTG TGTGGTGTTA CGTAGTAACA TCGGAACGGT GTATGAGCGC 
TGGTGGTATG AGAAGCTCAT CAACATGACC TACTGTCCCA AGACCAAGGT GTTGTGCTTG 
TGGCGTAGAA ATGGCTCTGA GACTCAGCTC AACAAATTCT ATACTAAGAA GTGTCGGGAG 
CTGTACTACT GTGTGAAGGA CAGCATGGAG CGGGCTGCTG CCCGGCAGCA AAGCATCAAA 
CCTGGCCCTG AACTGGGTGG CGAGTTCCCT GTGCAGGACA TGAAGACTGG TGAGGGTGGC 
TTGCTGCAGG TCACCCTAGA AGGGATCAAT CTCAAGTTCA TGCACAACCA GGAGCGGAAG 
GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TCCGAGGCGT CTTTGTCCTG 
GAGGAATTTG TTCCTGAAAT TAAAGAAGTG GTGAGCCACA AGTACAAGAC ACCAATGGCC 
CATGAGATCT GCTACTCTGT GTTGTGTCTC TTCTCGTATG TGGCTGCAGT TCGTAGCAGT 
GAGGAAGATC TCAGGACCCC ACCCCGGCCC GTCTCGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121185.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976660.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCe41240
Clone ID Related Accession (Same CDS sequence)	XM_014121185.2 , XM_014121185.1
Accession Version	XM_014121185.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121185.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGCAAAGAAC GTTCTACTTT GTGGGACCAA 
ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG 
AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG 
GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG 
CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG 
TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT 
GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG 
GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC 
TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT 
CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976660.1
CDS	234..5126
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121185.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121185.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGCAAAGAAC GTTCTACTTT GTGGGACCAA 
ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG 
AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG 
GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG 
CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG 
TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT 
GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG 
GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC 
TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT 
CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121185.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976660.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X11, mRNA.

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CloneID	OCe41240
Clone ID Related Accession (Same CDS sequence)	XM_014121185.2 , XM_014121185.1
Accession Version	XM_014121185.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGCAAAGAAC GTTCTACTTT GTGGGACCAA 
ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG 
AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG 
GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG 
CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG 
TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT 
GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG 
GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC 
TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT 
CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976660.1
CDS	255..5147
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121185.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121185.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGCAAAGAAC GTTCTACTTT GTGGGACCAA 
ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG 
AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG 
GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG 
CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG 
TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT 
GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG 
GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGGAGCGGAA GGTTTTCATA 
GAGCTGAATC ACATTAAAAA GTGCAATACA GTCCGAGGCG TCTTTGTCCT GGAGGAATTT 
GTTCCTGAAA TTAAAGAAGT GGTGAGCCAC AAGTACAAGA CACCAATGGC CCATGAGATC 
TGCTACTCTG TGTTGTGTCT CTTCTCGTAT GTGGCTGCAG TTCGTAGCAG TGAGGAAGAT 
CTCAGGACCC CACCCCGGCC CGTCTCGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121186.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976661.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X13, mRNA.

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CloneID	OCe41241
Clone ID Related Accession (Same CDS sequence)	XM_014121186.1 , XM_014121186.2
Accession Version	XM_014121186.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT 
GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGAATTG 
TGGAACAAGC ACCAGGAAGT GAAAAAGCAA AAAGCTTTGG AAAAACAGAG GCCTGAAGTC 
ATCAAACCCG TCTTTGACCT TGGTGAGACA GAGGAGAAAA AGTCCCAGAT CAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGGTTCCCAG AGGACTGATA CAGACTCTGT CATTGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGACTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCTG GAGAGACCCT TGGAGCAGAT AGTGACCTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGTG AAGGCAGCGC CCACTTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ATTCTGCTAC CAGCACCATC TTTGGGAAAG CCCATAGCTT GAAGCCAAGT 
GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCCG AAGATGTAAG CCAACGGGTC 
TATCTGTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAT 
GCGGCTATGG AGACCTTTTC TATAAGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC 
CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC 
CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT 
GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG 
GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC 
CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG 
ACCCCACCCC GGCCCGTCTC GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976661.1
CDS	254..5080
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121186.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121186.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT 
GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGAATTG 
TGGAACAAGC ACCAGGAAGT GAAAAAGCAA AAAGCTTTGG AAAAACAGAG GCCTGAAGTC 
ATCAAACCCG TCTTTGACCT TGGTGAGACA GAGGAGAAAA AGTCCCAGAT CAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGGTTCCCAG AGGACTGATA CAGACTCTGT CATTGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGACTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCTG GAGAGACCCT TGGAGCAGAT AGTGACCTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGTG AAGGCAGCGC CCACTTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ATTCTGCTAC CAGCACCATC TTTGGGAAAG CCCATAGCTT GAAGCCAAGT 
GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCCG AAGATGTAAG CCAACGGGTC 
TATCTGTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAT 
GCGGCTATGG AGACCTTTTC TATAAGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC 
CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC 
CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT 
GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG 
GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC 
CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG 
ACCCCACCCC GGCCCGTCTC GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121186.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976661.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X14, mRNA.

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CloneID	OCe41241
Clone ID Related Accession (Same CDS sequence)	XM_014121186.1 , XM_014121186.2
Accession Version	XM_014121186.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121186.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT 
GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGAATTG 
TGGAACAAGC ACCAGGAAGT GAAAAAGCAA AAAGCTTTGG AAAAACAGAG GCCTGAAGTC 
ATCAAACCCG TCTTTGACCT TGGTGAGACA GAGGAGAAAA AGTCCCAGAT CAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGGTTCCCAG AGGACTGATA CAGACTCTGT CATTGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGACTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCTG GAGAGACCCT TGGAGCAGAT AGTGACCTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGTG AAGGCAGCGC CCACTTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ATTCTGCTAC CAGCACCATC TTTGGGAAAG CCCATAGCTT GAAGCCAAGT 
GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCCG AAGATGTAAG CCAACGGGTC 
TATCTGTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAT 
GCGGCTATGG AGACCTTTTC TATAAGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC 
CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC 
CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT 
GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG 
GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC 
CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG 
ACCCCACCCC GGCCCGTCTC GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976661.1
CDS	234..5060
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121186.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121186.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT 
GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGAATTG 
TGGAACAAGC ACCAGGAAGT GAAAAAGCAA AAAGCTTTGG AAAAACAGAG GCCTGAAGTC 
ATCAAACCCG TCTTTGACCT TGGTGAGACA GAGGAGAAAA AGTCCCAGAT CAGCGCAGAC 
AGTGGTGTGA GCCTGACATC TGGTTCCCAG AGGACTGATA CAGACTCTGT CATTGGTGTG 
AGTCCAGCTG TTATGATCCG AAGCTCAAGT CAGGACTCTG AAGTTAGCAC CGTGGTGAGT 
AATAGTTCTG GAGAGACCCT TGGAGCAGAT AGTGACCTGA GCAGCAATGC AGGTGATGGA 
CCAGGTGGTG AAGGCAGCGC CCACTTGGCA AGCTCTCGGG GCACTTTGTC TGATAGTGAA 
ATTGAGACCA ATTCTGCTAC CAGCACCATC TTTGGGAAAG CCCATAGCTT GAAGCCAAGT 
GTAAAGGAGA AGCTGGTGGG CAGCCCAGTT CGCTCTTCCG AAGATGTAAG CCAACGGGTC 
TATCTGTACG AGGGACTCCT AGGAAGGGAC AAAGGATCGA TGTGGGACCA GTTAGAGGAT 
GCGGCTATGG AGACCTTTTC TATAAGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC 
CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC 
CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT 
GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG 
GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC 
CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGAGC GGAAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG 
ACCCCACCCC GGCCCGTCTC GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121187.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976662.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X14, mRNA.

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CloneID	OCe41242
Clone ID Related Accession (Same CDS sequence)	XM_014121187.1
Accession Version	XM_014121187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTCTG GACTCACATG 
GCAGTAACTC AAACCTCGGC GCTCCAGAGG AGGGACTTAG AAGAGCCACC TCTGCAGAGA 
GGTCAGGAGG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976662.1
CDS	255..5069
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVWTHMAVTQTSALQRRDLEEPPLQRGQEA

Target ORF information:

RefSeq Version	XM_014121187.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121187.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTCTG GACTCACATG 
GCAGTAACTC AAACCTCGGC GCTCCAGAGG AGGGACTTAG AAGAGCCACC TCTGCAGAGA 
GGTCAGGAGG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121188.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976663.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OCe41243
Clone ID Related Accession (Same CDS sequence)	XM_014121188.1 , XM_014121188.2
Accession Version	XM_014121188.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGCTTGA AACTGGCAAG CGACTCAGAC GCAGAGTCAG ACTCTCGAGC GAGCTCTCCC 
ACCTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTGTATC GAAACCACAG TACAAGCTTC AGTCTTTCAA ACCTCACACT GCCGACCAAA 
GGTGCCCGAG AGAAGACGAC CCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA 
ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCCTTAGTG 
GACCAGAAGT CATCTGTTAT TAAACACAGC CCAACAGTGA AAAGAGAGCC TCCATCACCT 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAAGT GGTACACAGC 
GTGCTGGATG GCCAGGGTGT TGGCTGGCTC AACATGAAAA AGGTGCGTCG GCTGCTGGAG 
AGTGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GAACTGTGCA GTCAGAAGAT 
GATGCCCGGC AGGACGTCAT CCCCGATGTG GAGATCAGCA GAAAGGTTTA CAAGGGGATG 
TTAGACCTGC TCAAGTGCAC AGTACTCAGC CTGGAGCAGT CCTATGCCCA CGCAGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTCTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAGAGTGTAA ATACACCAGT TGGCAAGGAT 
CCTGGCCTGG CTGGGCGGGG GGACCCAAAA GCTATGGCAC AGCTGAGAGT TCCCCAGCTG 
GGCCCTCGGG CGCCAAGTGC TGCAGGAAAG GGTCCCAAAG AACTAGACAC CAGAAGTTTA 
AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAAGCTT TGGAAAAACA GAGGCCTGAA GTCATCAAAC CCGTCTTTGA CCTTGGTGAG 
ACAGAGGAGA AAAAGTCCCA GATCAGCGCA GACAGTGGTG TGAGCCTGAC ATCTGGTTCC 
CAGAGGACTG ATACAGACTC TGTCATTGGT GTGAGTCCAG CTGTTATGAT CCGAAGCTCA 
AGTCAGGACT CTGAAGTTAG CACCGTGGTG AGTAATAGTT CTGGAGAGAC CCTTGGAGCA 
GATAGTGACC TGAGCAGCAA TGCAGGTGAT GGACCAGGTG GTGAAGGCAG CGCCCACTTG 
GCAAGCTCTC GGGGCACTTT GTCTGATAGT GAAATTGAGA CCAATTCTGC TACCAGCACC 
ATCTTTGGGA AAGCCCATAG CTTGAAGCCA AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA 
GTTCGCTCTT CCGAAGATGT AAGCCAACGG GTCTATCTGT ACGAGGGACT CCTAGGAAGG 
GACAAAGGAT CGATGTGGGA CCAGTTAGAG GATGCGGCTA TGGAGACCTT TTCTATAAGC 
AAAGAACGTT CTACTTTGTG GGACCAAATG CAGTTCTGGG AAGATGCATT CTTAGATGCT 
GTGATGTTGG AGAGAGAAGG GATGGGTATG GACCAGGGTC CCCAGGAAAT GATCGACAGG 
TACCTGTCCC TGGGAGAACA TGACCGGAAG CGCCTAGAGG ATGATGAAGA TCGTTTGCTG 
GCCACACTTT TGCACAACCT CATCTCCTAC ATGCTGCTGA TGAAGGTAAA TAAGAATGAC 
ATCCGGAAGA AGGTGAGGCG CCTAATGGGA AAGTCCCATA TTGGGCTTGT GTACAGCCAG 
CAAATCAATG AAGTGCTTGA TCAGCTGGTG AACCTGAATG GACGTGATCT CTCTATCCGG 
TCCAGTGGCA GCCGGCACAT GAAAAAGCAG ACATTTGTGG TACATGCAGG GACAGACACA 
AATGGAGACA TCTTCTTCAT GGAGGTGTGT GATGACTGTG TGGTGTTACG TAGTAACATC 
GGAACGGTGT ATGAGCGCTG GTGGTATGAG AAGCTCATCA ACATGACCTA CTGTCCCAAG 
ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT GGCTCTGAGA CTCAGCTCAA CAAATTCTAT 
ACTAAGAAGT GTCGGGAGCT GTACTACTGT GTGAAGGACA GCATGGAGCG GGCTGCTGCC 
CGGCAGCAAA GCATCAAACC TGGCCCTGAA CTGGGTGGCG AGTTCCCTGT GCAGGACATG 
AAGACTGGTG AGGGTGGCTT GCTGCAGGTC ACCCTAGAAG GGATCAATCT CAAGTTCATG 
CACAACCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTC 
CGAGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAATTA AAGAAGTGGT GAGCCACAAG 
TACAAGACAC CAATGGCCCA TGAGATCTGC TACTCTGTGT TGTGTCTCTT CTCGTATGTG 
GCTGCAGTTC GTAGCAGTGA GGAAGATCTC AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976663.1
CDS	254..5053
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121188.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121188.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGCTTGA AACTGGCAAG CGACTCAGAC GCAGAGTCAG ACTCTCGAGC GAGCTCTCCC 
ACCTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTGTATC GAAACCACAG TACAAGCTTC AGTCTTTCAA ACCTCACACT GCCGACCAAA 
GGTGCCCGAG AGAAGACGAC CCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA 
ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCCTTAGTG 
GACCAGAAGT CATCTGTTAT TAAACACAGC CCAACAGTGA AAAGAGAGCC TCCATCACCT 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAAGT GGTACACAGC 
GTGCTGGATG GCCAGGGTGT TGGCTGGCTC AACATGAAAA AGGTGCGTCG GCTGCTGGAG 
AGTGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GAACTGTGCA GTCAGAAGAT 
GATGCCCGGC AGGACGTCAT CCCCGATGTG GAGATCAGCA GAAAGGTTTA CAAGGGGATG 
TTAGACCTGC TCAAGTGCAC AGTACTCAGC CTGGAGCAGT CCTATGCCCA CGCAGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTCTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAGAGTGTAA ATACACCAGT TGGCAAGGAT 
CCTGGCCTGG CTGGGCGGGG GGACCCAAAA GCTATGGCAC AGCTGAGAGT TCCCCAGCTG 
GGCCCTCGGG CGCCAAGTGC TGCAGGAAAG GGTCCCAAAG AACTAGACAC CAGAAGTTTA 
AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAAGCTT TGGAAAAACA GAGGCCTGAA GTCATCAAAC CCGTCTTTGA CCTTGGTGAG 
ACAGAGGAGA AAAAGTCCCA GATCAGCGCA GACAGTGGTG TGAGCCTGAC ATCTGGTTCC 
CAGAGGACTG ATACAGACTC TGTCATTGGT GTGAGTCCAG CTGTTATGAT CCGAAGCTCA 
AGTCAGGACT CTGAAGTTAG CACCGTGGTG AGTAATAGTT CTGGAGAGAC CCTTGGAGCA 
GATAGTGACC TGAGCAGCAA TGCAGGTGAT GGACCAGGTG GTGAAGGCAG CGCCCACTTG 
GCAAGCTCTC GGGGCACTTT GTCTGATAGT GAAATTGAGA CCAATTCTGC TACCAGCACC 
ATCTTTGGGA AAGCCCATAG CTTGAAGCCA AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA 
GTTCGCTCTT CCGAAGATGT AAGCCAACGG GTCTATCTGT ACGAGGGACT CCTAGGAAGG 
GACAAAGGAT CGATGTGGGA CCAGTTAGAG GATGCGGCTA TGGAGACCTT TTCTATAAGC 
AAAGAACGTT CTACTTTGTG GGACCAAATG CAGTTCTGGG AAGATGCATT CTTAGATGCT 
GTGATGTTGG AGAGAGAAGG GATGGGTATG GACCAGGGTC CCCAGGAAAT GATCGACAGG 
TACCTGTCCC TGGGAGAACA TGACCGGAAG CGCCTAGAGG ATGATGAAGA TCGTTTGCTG 
GCCACACTTT TGCACAACCT CATCTCCTAC ATGCTGCTGA TGAAGGTAAA TAAGAATGAC 
ATCCGGAAGA AGGTGAGGCG CCTAATGGGA AAGTCCCATA TTGGGCTTGT GTACAGCCAG 
CAAATCAATG AAGTGCTTGA TCAGCTGGTG AACCTGAATG GACGTGATCT CTCTATCCGG 
TCCAGTGGCA GCCGGCACAT GAAAAAGCAG ACATTTGTGG TACATGCAGG GACAGACACA 
AATGGAGACA TCTTCTTCAT GGAGGTGTGT GATGACTGTG TGGTGTTACG TAGTAACATC 
GGAACGGTGT ATGAGCGCTG GTGGTATGAG AAGCTCATCA ACATGACCTA CTGTCCCAAG 
ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT GGCTCTGAGA CTCAGCTCAA CAAATTCTAT 
ACTAAGAAGT GTCGGGAGCT GTACTACTGT GTGAAGGACA GCATGGAGCG GGCTGCTGCC 
CGGCAGCAAA GCATCAAACC TGGCCCTGAA CTGGGTGGCG AGTTCCCTGT GCAGGACATG 
AAGACTGGTG AGGGTGGCTT GCTGCAGGTC ACCCTAGAAG GGATCAATCT CAAGTTCATG 
CACAACCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTC 
CGAGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAATTA AAGAAGTGGT GAGCCACAAG 
TACAAGACAC CAATGGCCCA TGAGATCTGC TACTCTGTGT TGTGTCTCTT CTCGTATGTG 
GCTGCAGTTC GTAGCAGTGA GGAAGATCTC AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121188.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976663.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OCe41243
Clone ID Related Accession (Same CDS sequence)	XM_014121188.1 , XM_014121188.2
Accession Version	XM_014121188.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121188.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGCTTGA AACTGGCAAG CGACTCAGAC GCAGAGTCAG ACTCTCGAGC GAGCTCTCCC 
ACCTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTGTATC GAAACCACAG TACAAGCTTC AGTCTTTCAA ACCTCACACT GCCGACCAAA 
GGTGCCCGAG AGAAGACGAC CCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA 
ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCCTTAGTG 
GACCAGAAGT CATCTGTTAT TAAACACAGC CCAACAGTGA AAAGAGAGCC TCCATCACCT 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAAGT GGTACACAGC 
GTGCTGGATG GCCAGGGTGT TGGCTGGCTC AACATGAAAA AGGTGCGTCG GCTGCTGGAG 
AGTGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GAACTGTGCA GTCAGAAGAT 
GATGCCCGGC AGGACGTCAT CCCCGATGTG GAGATCAGCA GAAAGGTTTA CAAGGGGATG 
TTAGACCTGC TCAAGTGCAC AGTACTCAGC CTGGAGCAGT CCTATGCCCA CGCAGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTCTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAGAGTGTAA ATACACCAGT TGGCAAGGAT 
CCTGGCCTGG CTGGGCGGGG GGACCCAAAA GCTATGGCAC AGCTGAGAGT TCCCCAGCTG 
GGCCCTCGGG CGCCAAGTGC TGCAGGAAAG GGTCCCAAAG AACTAGACAC CAGAAGTTTA 
AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAAGCTT TGGAAAAACA GAGGCCTGAA GTCATCAAAC CCGTCTTTGA CCTTGGTGAG 
ACAGAGGAGA AAAAGTCCCA GATCAGCGCA GACAGTGGTG TGAGCCTGAC ATCTGGTTCC 
CAGAGGACTG ATACAGACTC TGTCATTGGT GTGAGTCCAG CTGTTATGAT CCGAAGCTCA 
AGTCAGGACT CTGAAGTTAG CACCGTGGTG AGTAATAGTT CTGGAGAGAC CCTTGGAGCA 
GATAGTGACC TGAGCAGCAA TGCAGGTGAT GGACCAGGTG GTGAAGGCAG CGCCCACTTG 
GCAAGCTCTC GGGGCACTTT GTCTGATAGT GAAATTGAGA CCAATTCTGC TACCAGCACC 
ATCTTTGGGA AAGCCCATAG CTTGAAGCCA AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA 
GTTCGCTCTT CCGAAGATGT AAGCCAACGG GTCTATCTGT ACGAGGGACT CCTAGGAAGG 
GACAAAGGAT CGATGTGGGA CCAGTTAGAG GATGCGGCTA TGGAGACCTT TTCTATAAGC 
AAAGAACGTT CTACTTTGTG GGACCAAATG CAGTTCTGGG AAGATGCATT CTTAGATGCT 
GTGATGTTGG AGAGAGAAGG GATGGGTATG GACCAGGGTC CCCAGGAAAT GATCGACAGG 
TACCTGTCCC TGGGAGAACA TGACCGGAAG CGCCTAGAGG ATGATGAAGA TCGTTTGCTG 
GCCACACTTT TGCACAACCT CATCTCCTAC ATGCTGCTGA TGAAGGTAAA TAAGAATGAC 
ATCCGGAAGA AGGTGAGGCG CCTAATGGGA AAGTCCCATA TTGGGCTTGT GTACAGCCAG 
CAAATCAATG AAGTGCTTGA TCAGCTGGTG AACCTGAATG GACGTGATCT CTCTATCCGG 
TCCAGTGGCA GCCGGCACAT GAAAAAGCAG ACATTTGTGG TACATGCAGG GACAGACACA 
AATGGAGACA TCTTCTTCAT GGAGGTGTGT GATGACTGTG TGGTGTTACG TAGTAACATC 
GGAACGGTGT ATGAGCGCTG GTGGTATGAG AAGCTCATCA ACATGACCTA CTGTCCCAAG 
ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT GGCTCTGAGA CTCAGCTCAA CAAATTCTAT 
ACTAAGAAGT GTCGGGAGCT GTACTACTGT GTGAAGGACA GCATGGAGCG GGCTGCTGCC 
CGGCAGCAAA GCATCAAACC TGGCCCTGAA CTGGGTGGCG AGTTCCCTGT GCAGGACATG 
AAGACTGGTG AGGGTGGCTT GCTGCAGGTC ACCCTAGAAG GGATCAATCT CAAGTTCATG 
CACAACCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTC 
CGAGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAATTA AAGAAGTGGT GAGCCACAAG 
TACAAGACAC CAATGGCCCA TGAGATCTGC TACTCTGTGT TGTGTCTCTT CTCGTATGTG 
GCTGCAGTTC GTAGCAGTGA GGAAGATCTC AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976663.1
CDS	234..5033
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_014121188.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121188.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGCTTGA AACTGGCAAG CGACTCAGAC GCAGAGTCAG ACTCTCGAGC GAGCTCTCCC 
ACCTCCACCG TCTCCAACAC CAGCACCGAG GGCTTCGGGG GCATCATGTC TTTTGCCAGC 
AGCCTGTATC GAAACCACAG TACAAGCTTC AGTCTTTCAA ACCTCACACT GCCGACCAAA 
GGTGCCCGAG AGAAGACGAC CCCCTTCCCC AGTCTGAAAG TATTTGGGCT AAATACTCTA 
ATGGAGATTG TTACTGAAGC CGGCCCCGGG AGTGGTGAAG GAAACAGGAG GGCCTTAGTG 
GACCAGAAGT CATCTGTTAT TAAACACAGC CCAACAGTGA AAAGAGAGCC TCCATCACCT 
CAGGGTCGAT CCAGCAATTC TAGTGAGAAC CAGCAGTTCC TGAAGGAAGT GGTACACAGC 
GTGCTGGATG GCCAGGGTGT TGGCTGGCTC AACATGAAAA AGGTGCGTCG GCTGCTGGAG 
AGTGAGCAGC TGCGAGTCTT TGTCCTGAGC AAGCTGAACC GAACTGTGCA GTCAGAAGAT 
GATGCCCGGC AGGACGTCAT CCCCGATGTG GAGATCAGCA GAAAGGTTTA CAAGGGGATG 
TTAGACCTGC TCAAGTGCAC AGTACTCAGC CTGGAGCAGT CCTATGCCCA CGCAGGTCTG 
GGTGGCATGG CCAGCATCTT TGGGCTTCTG GAGATTGCCC AGACCCACTA CTATAGTAAA 
GAACCAGACA AGCGGAAGAG AAGTCCAACA GAGAGTGTAA ATACACCAGT TGGCAAGGAT 
CCTGGCCTGG CTGGGCGGGG GGACCCAAAA GCTATGGCAC AGCTGAGAGT TCCCCAGCTG 
GGCCCTCGGG CGCCAAGTGC TGCAGGAAAG GGTCCCAAAG AACTAGACAC CAGAAGTTTA 
AAGGAAGAGA ATTTTGTAGC ATCTGTTGAA TTGTGGAACA AGCACCAGGA AGTGAAAAAG 
CAAAAAGCTT TGGAAAAACA GAGGCCTGAA GTCATCAAAC CCGTCTTTGA CCTTGGTGAG 
ACAGAGGAGA AAAAGTCCCA GATCAGCGCA GACAGTGGTG TGAGCCTGAC ATCTGGTTCC 
CAGAGGACTG ATACAGACTC TGTCATTGGT GTGAGTCCAG CTGTTATGAT CCGAAGCTCA 
AGTCAGGACT CTGAAGTTAG CACCGTGGTG AGTAATAGTT CTGGAGAGAC CCTTGGAGCA 
GATAGTGACC TGAGCAGCAA TGCAGGTGAT GGACCAGGTG GTGAAGGCAG CGCCCACTTG 
GCAAGCTCTC GGGGCACTTT GTCTGATAGT GAAATTGAGA CCAATTCTGC TACCAGCACC 
ATCTTTGGGA AAGCCCATAG CTTGAAGCCA AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA 
GTTCGCTCTT CCGAAGATGT AAGCCAACGG GTCTATCTGT ACGAGGGACT CCTAGGAAGG 
GACAAAGGAT CGATGTGGGA CCAGTTAGAG GATGCGGCTA TGGAGACCTT TTCTATAAGC 
AAAGAACGTT CTACTTTGTG GGACCAAATG CAGTTCTGGG AAGATGCATT CTTAGATGCT 
GTGATGTTGG AGAGAGAAGG GATGGGTATG GACCAGGGTC CCCAGGAAAT GATCGACAGG 
TACCTGTCCC TGGGAGAACA TGACCGGAAG CGCCTAGAGG ATGATGAAGA TCGTTTGCTG 
GCCACACTTT TGCACAACCT CATCTCCTAC ATGCTGCTGA TGAAGGTAAA TAAGAATGAC 
ATCCGGAAGA AGGTGAGGCG CCTAATGGGA AAGTCCCATA TTGGGCTTGT GTACAGCCAG 
CAAATCAATG AAGTGCTTGA TCAGCTGGTG AACCTGAATG GACGTGATCT CTCTATCCGG 
TCCAGTGGCA GCCGGCACAT GAAAAAGCAG ACATTTGTGG TACATGCAGG GACAGACACA 
AATGGAGACA TCTTCTTCAT GGAGGTGTGT GATGACTGTG TGGTGTTACG TAGTAACATC 
GGAACGGTGT ATGAGCGCTG GTGGTATGAG AAGCTCATCA ACATGACCTA CTGTCCCAAG 
ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT GGCTCTGAGA CTCAGCTCAA CAAATTCTAT 
ACTAAGAAGT GTCGGGAGCT GTACTACTGT GTGAAGGACA GCATGGAGCG GGCTGCTGCC 
CGGCAGCAAA GCATCAAACC TGGCCCTGAA CTGGGTGGCG AGTTCCCTGT GCAGGACATG 
AAGACTGGTG AGGGTGGCTT GCTGCAGGTC ACCCTAGAAG GGATCAATCT CAAGTTCATG 
CACAACCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTC 
CGAGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAATTA AAGAAGTGGT GAGCCACAAG 
TACAAGACAC CAATGGCCCA TGAGATCTGC TACTCTGTGT TGTGTCTCTT CTCGTATGTG 
GCTGCAGTTC GTAGCAGTGA GGAAGATCTC AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121189.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976664.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X19, mRNA.

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CloneID	OCe41244
Clone ID Related Accession (Same CDS sequence)	XM_014121189.1 , XM_014121189.2
Accession Version	XM_014121189.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA 
GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA 
ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG 
AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG 
GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG 
CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG 
TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT 
GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG 
GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGTTCCTGAA ATTAAAGAAG 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976664.1
CDS	254..4999
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_014121189.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121189.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA 
GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA 
ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG 
AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG 
GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG 
CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG 
TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT 
GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG 
GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGTTCCTGAA ATTAAAGAAG 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121189.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976664.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X19, mRNA.

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CloneID	OCe41244
Clone ID Related Accession (Same CDS sequence)	XM_014121189.1 , XM_014121189.2
Accession Version	XM_014121189.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121189.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA 
GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA 
ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG 
AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG 
GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG 
CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG 
TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT 
GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG 
GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGTTCCTGAA ATTAAAGAAG 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976664.1
CDS	234..4979
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_014121189.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121189.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGA AGGGACAAAG GATCGATGTG GGACCAGTTA 
GAGGATGCGG CTATGGAGAC CTTTTCTATA AGCAAAGAAC GTTCTACTTT GTGGGACCAA 
ATGCAGTTCT GGGAAGATGC ATTCTTAGAT GCTGTGATGT TGGAGAGAGA AGGGATGGGT 
ATGGACCAGG GTCCCCAGGA AATGATCGAC AGGTACCTGT CCCTGGGAGA ACATGACCGG 
AAGCGCCTAG AGGATGATGA AGATCGTTTG CTGGCCACAC TTTTGCACAA CCTCATCTCC 
TACATGCTGC TGATGAAGGT AAATAAGAAT GACATCCGGA AGAAGGTGAG GCGCCTAATG 
GGAAAGTCCC ATATTGGGCT TGTGTACAGC CAGCAAATCA ATGAAGTGCT TGATCAGCTG 
GTGAACCTGA ATGGACGTGA TCTCTCTATC CGGTCCAGTG GCAGCCGGCA CATGAAAAAG 
CAGACATTTG TGGTACATGC AGGGACAGAC ACAAATGGAG ACATCTTCTT CATGGAGGTG 
TGTGATGACT GTGTGGTGTT ACGTAGTAAC ATCGGAACGG TGTATGAGCG CTGGTGGTAT 
GAGAAGCTCA TCAACATGAC CTACTGTCCC AAGACCAAGG TGTTGTGCTT GTGGCGTAGA 
AATGGCTCTG AGACTCAGCT CAACAAATTC TATACTAAGA AGTGTCGGGA GCTGTACTAC 
TGTGTGAAGG ACAGCATGGA GCGGGCTGCT GCCCGGCAGC AAAGCATCAA ACCTGGCCCT 
GAACTGGGTG GCGAGTTCCC TGTGCAGGAC ATGAAGACTG GTGAGGGTGG CTTGCTGCAG 
GTCACCCTAG AAGGGATCAA TCTCAAGTTC ATGCACAACC AGTTCCTGAA ATTAAAGAAG 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005631241.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631298.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X6, mRNA.

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CloneID	OCe20065
Clone ID Related Accession (Same CDS sequence)	XM_005631241.2 , XM_005631241.3
Accession Version	XM_005631241.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631241.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG 
ACCCCACCCC GGCCCGTCTC GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631298.1
CDS	254..5200
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005631241.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631241.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG 
ACCCCACCCC GGCCCGTCTC GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005631241.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631298.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

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CloneID	OCe20065
Clone ID Related Accession (Same CDS sequence)	XM_005631241.2 , XM_005631241.3
Accession Version	XM_005631241.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631241.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG 
ACCCCACCCC GGCCCGTCTC GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631298.1
CDS	234..5180
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005631241.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631241.3

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG 
ACCCCACCCC GGCCCGTCTC GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005631243.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631300.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe20066
Clone ID Related Accession (Same CDS sequence)	XM_005631243.3 , XM_005631243.2
Accession Version	XM_005631243.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631243.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631300.1
CDS	234..5129
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005631243.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631243.3

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005631243.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631300.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X10, mRNA.

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CloneID	OCe20066
Clone ID Related Accession (Same CDS sequence)	XM_005631243.3 , XM_005631243.2
Accession Version	XM_005631243.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631243.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631300.1
CDS	254..5149
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005631243.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631243.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGAGCG GAAGGTTTTC 
ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTCCGAG GCGTCTTTGT CCTGGAGGAA 
TTTGTTCCTG AAATTAAAGA AGTGGTGAGC CACAAGTACA AGACACCAAT GGCCCATGAG 
ATCTGCTACT CTGTGTTGTG TCTCTTCTCG TATGTGGCTG CAGTTCGTAG CAGTGAGGAA 
GATCTCAGGA CCCCACCCCG GCCCGTCTCG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_533189.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_533189.2 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X12, mRNA.

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CloneID	OCe20067
Clone ID Related Accession (Same CDS sequence)	XM_533189.5 , XM_533189.6
Accession Version	XM_533189.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_533189.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC 
AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT 
TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT 
AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT 
GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG 
GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT 
GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT 
CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG 
AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG 
GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG 
TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT 
GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG 
CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC 
TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC 
AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_533189.2
CDS	255..5084
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_533189.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_533189.5

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC 
AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT 
TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT 
AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT 
GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG 
GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT 
GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT 
CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG 
AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG 
GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG 
TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT 
GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG 
CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC 
TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC 
AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_533189.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_533189.2 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

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CloneID	OCe20067
Clone ID Related Accession (Same CDS sequence)	XM_533189.5 , XM_533189.6
Accession Version	XM_533189.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_533189.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC 
AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT 
TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT 
AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT 
GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG 
GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT 
GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT 
CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG 
AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG 
GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG 
TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT 
GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG 
CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC 
TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC 
AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_533189.2
CDS	234..5063
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_533189.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_533189.6

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC 
AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT 
TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT 
AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT 
GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG 
GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT 
GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT 
CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG 
AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG 
GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG 
TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT 
GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG 
CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC 
TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC 
AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_855751.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_860844.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X16, mRNA.

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CloneID	OCe20069
Clone ID Related Accession (Same CDS sequence)	XM_855751.5 , XM_855751.4
Accession Version	XM_855751.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_855751.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC 
AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT 
TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT 
AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT 
GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG 
GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT 
GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT 
CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG 
AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG 
GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG 
TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT 
GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG 
CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC 
TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC 
AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_860844.1
CDS	234..5003
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_855751.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_855751.5

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC 
AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT 
TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT 
AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT 
GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG 
GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT 
GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT 
CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG 
AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG 
GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG 
TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT 
GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG 
CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC 
TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC 
AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_855751.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_860844.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OCe20069
Clone ID Related Accession (Same CDS sequence)	XM_855751.5 , XM_855751.4
Accession Version	XM_855751.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_855751.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC 
AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT 
TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT 
AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT 
GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG 
GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT 
GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT 
CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG 
AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG 
GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG 
TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT 
GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG 
CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC 
TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC 
AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_860844.1
CDS	255..5024
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_855751.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_855751.4

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGG TGAGTAATAG TTCTGGAGAG ACCCTTGGAG CAGATAGTGA CCTGAGCAGC 
AATGCAGGTG ATGGACCAGG TGGTGAAGGC AGCGCCCACT TGGCAAGCTC TCGGGGCACT 
TTGTCTGATA GTGAAATTGA GACCAATTCT GCTACCAGCA CCATCTTTGG GAAAGCCCAT 
AGCTTGAAGC CAAGTGTAAA GGAGAAGCTG GTGGGCAGCC CAGTTCGCTC TTCCGAAGAT 
GTAAGCCAAC GGGTCTATCT GTACGAGGGA CTCCTAGGCA AAGAACGTTC TACTTTGTGG 
GACCAAATGC AGTTCTGGGA AGATGCATTC TTAGATGCTG TGATGTTGGA GAGAGAAGGG 
ATGGGTATGG ACCAGGGTCC CCAGGAAATG ATCGACAGGT ACCTGTCCCT GGGAGAACAT 
GACCGGAAGC GCCTAGAGGA TGATGAAGAT CGTTTGCTGG CCACACTTTT GCACAACCTC 
ATCTCCTACA TGCTGCTGAT GAAGGTAAAT AAGAATGACA TCCGGAAGAA GGTGAGGCGC 
CTAATGGGAA AGTCCCATAT TGGGCTTGTG TACAGCCAGC AAATCAATGA AGTGCTTGAT 
CAGCTGGTGA ACCTGAATGG ACGTGATCTC TCTATCCGGT CCAGTGGCAG CCGGCACATG 
AAAAAGCAGA CATTTGTGGT ACATGCAGGG ACAGACACAA ATGGAGACAT CTTCTTCATG 
GAGGTGTGTG ATGACTGTGT GGTGTTACGT AGTAACATCG GAACGGTGTA TGAGCGCTGG 
TGGTATGAGA AGCTCATCAA CATGACCTAC TGTCCCAAGA CCAAGGTGTT GTGCTTGTGG 
CGTAGAAATG GCTCTGAGAC TCAGCTCAAC AAATTCTATA CTAAGAAGTG TCGGGAGCTG 
TACTACTGTG TGAAGGACAG CATGGAGCGG GCTGCTGCCC GGCAGCAAAG CATCAAACCT 
GGCCCTGAAC TGGGTGGCGA GTTCCCTGTG CAGGACATGA AGACTGGTGA GGGTGGCTTG 
CTGCAGGTCA CCCTAGAAGG GATCAATCTC AAGTTCATGC ACAACCAGGT TTTCATAGAG 
CTGAATCACA TTAAAAAGTG CAATACAGTC CGAGGCGTCT TTGTCCTGGA GGAATTTGTT 
CCTGAAATTA AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA TGAGATCTGC 
TACTCTGTGT TGTGTCTCTT CTCGTATGTG GCTGCAGTTC GTAGCAGTGA GGAAGATCTC 
AGGACCCCAC CCCGGCCCGT CTCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005631246.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631303.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OCe20070
Clone ID Related Accession (Same CDS sequence)	XM_005631246.2 , XM_005631246.3
Accession Version	XM_005631246.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631246.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG 
AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631303.1
CDS	254..5002
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_005631246.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631246.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG 
AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005631246.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631303.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X18, mRNA.

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CloneID	OCe20070
Clone ID Related Accession (Same CDS sequence)	XM_005631246.2 , XM_005631246.3
Accession Version	XM_005631246.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631246.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG 
AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631303.1
CDS	234..4982
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_005631246.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631246.3

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGTATT TGGGCTAAAT ACTCTAATGG AGATTGTTAC TGAAGCCGGC 
CCCGGGAGTG GTGAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG 
AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005631247.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631304.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

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CloneID	OCe20071
Clone ID Related Accession (Same CDS sequence)	XM_005631247.3 , XM_005631247.2
Accession Version	XM_005631247.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631247.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGA GTAATAGTTC TGGAGAGACC CTTGGAGCAG ATAGTGACCT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG TGAAGGCAGC GCCCACTTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAATTCTGCT ACCAGCACCA TCTTTGGGAA AGCCCATAGC 
TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTCTTC CGAAGATGTA 
AGCCAACGGG TCTATCTGTA CGAGGGACTC CTAGGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG 
ACCCCACCCC GGCCCGTCTC GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631304.1
CDS	234..5000
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005631247.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631247.3

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGA GTAATAGTTC TGGAGAGACC CTTGGAGCAG ATAGTGACCT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG TGAAGGCAGC GCCCACTTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAATTCTGCT ACCAGCACCA TCTTTGGGAA AGCCCATAGC 
TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTCTTC CGAAGATGTA 
AGCCAACGGG TCTATCTGTA CGAGGGACTC CTAGGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG 
ACCCCACCCC GGCCCGTCTC GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005631247.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631304.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X17, mRNA.

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CloneID	OCe20071
Clone ID Related Accession (Same CDS sequence)	XM_005631247.3 , XM_005631247.2
Accession Version	XM_005631247.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGA GTAATAGTTC TGGAGAGACC CTTGGAGCAG ATAGTGACCT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG TGAAGGCAGC GCCCACTTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAATTCTGCT ACCAGCACCA TCTTTGGGAA AGCCCATAGC 
TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTCTTC CGAAGATGTA 
AGCCAACGGG TCTATCTGTA CGAGGGACTC CTAGGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG 
ACCCCACCCC GGCCCGTCTC GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631304.1
CDS	255..5021
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005631247.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631247.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGGGCCTG AAGTCATCAA ACCCGTCTTT GACCTTGGTG AGACAGAGGA GAAAAAGTCC 
CAGATCAGCG CAGACAGTGG TGTGAGCCTG ACATCTGGTT CCCAGAGGAC TGATACAGAC 
TCTGTCATTG GTGTGAGTCC AGCTGTTATG ATCCGAAGCT CAAGTCAGGA CTCTGAAGTT 
AGCACCGTGA GTAATAGTTC TGGAGAGACC CTTGGAGCAG ATAGTGACCT GAGCAGCAAT 
GCAGGTGATG GACCAGGTGG TGAAGGCAGC GCCCACTTGG CAAGCTCTCG GGGCACTTTG 
TCTGATAGTG AAATTGAGAC CAATTCTGCT ACCAGCACCA TCTTTGGGAA AGCCCATAGC 
TTGAAGCCAA GTGTAAAGGA GAAGCTGGTG GGCAGCCCAG TTCGCTCTTC CGAAGATGTA 
AGCCAACGGG TCTATCTGTA CGAGGGACTC CTAGGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGGTTTT CATAGAGCTG 
AATCACATTA AAAAGTGCAA TACAGTCCGA GGCGTCTTTG TCCTGGAGGA ATTTGTTCCT 
GAAATTAAAG AAGTGGTGAG CCACAAGTAC AAGACACCAA TGGCCCATGA GATCTGCTAC 
TCTGTGTTGT GTCTCTTCTC GTATGTGGCT GCAGTTCGTA GCAGTGAGGA AGATCTCAGG 
ACCCCACCCC GGCCCGTCTC GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_855777.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_860870.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OCe20072
Clone ID Related Accession (Same CDS sequence)	XM_855777.4 , XM_855777.5
Accession Version	XM_855777.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X17
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_855777.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT 
GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG 
CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG 
AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG 
ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT 
GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA 
CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC 
ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA 
CCCCGGCCCG TCTCGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_860870.1
CDS	255..4955
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_855777.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_855777.4

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT 
GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG 
CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG 
AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG 
ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT 
GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA 
CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC 
ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA 
CCCCGGCCCG TCTCGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_855777.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_860870.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OCe20072
Clone ID Related Accession (Same CDS sequence)	XM_855777.4 , XM_855777.5
Accession Version	XM_855777.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_855777.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT 
GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG 
CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG 
AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG 
ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT 
GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA 
CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC 
ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA 
CCCCGGCCCG TCTCGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_860870.1
CDS	234..4934
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_855777.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_855777.5

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGTAT TTGGGCTAAA TACTCTAATG GAGATTGTTA CTGAAGCCGG CCCCGGGAGT 
GGTGAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG 
CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG 
AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG 
ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT 
GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA 
CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC 
ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA 
CCCCGGCCCG TCTCGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121190.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976665.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X21, mRNA.

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CloneID	OCe20073
Clone ID Related Accession (Same CDS sequence)	XM_014121190.1 , XM_014121190.2
Accession Version	XM_014121190.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X18
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG 
AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976665.1
CDS	254..4942
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_014121190.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121190.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG 
AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121190.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976665.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X21, mRNA.

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CloneID	OCe20073
Clone ID Related Accession (Same CDS sequence)	XM_014121190.1 , XM_014121190.2
Accession Version	XM_014121190.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121190.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG 
AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976665.1
CDS	234..4922
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGSVRRRTYDNPYFEPQYGFPPEEEDDEQGESYTPRFSQHVNGNRAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_014121190.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121190.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGTCAG TGCGCCGGCG AACCTATGAC AATCCATACT TCGAGCCCCA ATATGGCTTT 
CCCCCTGAGG AGGAGGATGA TGAGCAGGGG GAAAGTTACA CTCCCCGATT CAGCCAACAT 
GTCAATGGCA ATCGGGCTCA AAAGATGCTG CGGCCCAACA GCTTGAAACT GGCAAGCGAC 
TCAGACGCAG AGTCAGACTC TCGAGCGAGC TCTCCCACCT CCACCGTCTC CAACACCAGC 
ACCGAGGGCT TCGGGGGCAT CATGTCTTTT GCCAGCAGCC TGTATCGAAA CCACAGTACA 
AGCTTCAGTC TTTCAAACCT CACACTGCCG ACCAAAGGTG CCCGAGAGAA GACGACCCCC 
TTCCCCAGTC TGAAAGGAAA CAGGAGGGCC TTAGTGGACC AGAAGTCATC TGTTATTAAA 
CACAGCCCAA CAGTGAAAAG AGAGCCTCCA TCACCTCAGG GTCGATCCAG CAATTCTAGT 
GAGAACCAGC AGTTCCTGAA GGAAGTGGTA CACAGCGTGC TGGATGGCCA GGGTGTTGGC 
TGGCTCAACA TGAAAAAGGT GCGTCGGCTG CTGGAGAGTG AGCAGCTGCG AGTCTTTGTC 
CTGAGCAAGC TGAACCGAAC TGTGCAGTCA GAAGATGATG CCCGGCAGGA CGTCATCCCC 
GATGTGGAGA TCAGCAGAAA GGTTTACAAG GGGATGTTAG ACCTGCTCAA GTGCACAGTA 
CTCAGCCTGG AGCAGTCCTA TGCCCACGCA GGTCTGGGTG GCATGGCCAG CATCTTTGGG 
CTTCTGGAGA TTGCCCAGAC CCACTACTAT AGTAAAGAAC CAGACAAGCG GAAGAGAAGT 
CCAACAGAGA GTGTAAATAC ACCAGTTGGC AAGGATCCTG GCCTGGCTGG GCGGGGGGAC 
CCAAAAGCTA TGGCACAGCT GAGAGTTCCC CAGCTGGGCC CTCGGGCGCC AAGTGCTGCA 
GGAAAGGGTC CCAAAGAACT AGACACCAGA AGTTTAAAGG AAGAGAATTT TGTAGCATCT 
GTTGAATTGT GGAACAAGCA CCAGGAAGTG AAAAAGCAAA AAGCTTTGGA AAAACAGAGG 
CCTGAAGTCA TCAAACCCGT CTTTGACCTT GGTGAGACAG AGGAGAAAAA GTCCCAGATC 
AGCGCAGACA GTGGTGTGAG CCTGACATCT GGTTCCCAGA GGACTGATAC AGACTCTGTC 
ATTGGTGTGA GTCCAGCTGT TATGATCCGA AGCTCAAGTC AGGACTCTGA AGTTAGCACC 
GTGGTGAGTA ATAGTTCTGG AGAGACCCTT GGAGCAGATA GTGACCTGAG CAGCAATGCA 
GGTGATGGAC CAGGTGGTGA AGGCAGCGCC CACTTGGCAA GCTCTCGGGG CACTTTGTCT 
GATAGTGAAA TTGAGACCAA TTCTGCTACC AGCACCATCT TTGGGAAAGC CCATAGCTTG 
AAGCCAAGTG TAAAGGAGAA GCTGGTGGGC AGCCCAGTTC GCTCTTCCGA AGATGTAAGC 
CAACGGGTCT ATCTGTACGA GGGACTCCTA GGAAGGGACA AAGGATCGAT GTGGGACCAG 
TTAGAGGATG CGGCTATGGA GACCTTTTCT ATAAGCAAAG AACGTTCTAC TTTGTGGGAC 
CAAATGCAGT TCTGGGAAGA TGCATTCTTA GATGCTGTGA TGTTGGAGAG AGAAGGGATG 
GGTATGGACC AGGGTCCCCA GGAAATGATC GACAGGTACC TGTCCCTGGG AGAACATGAC 
CGGAAGCGCC TAGAGGATGA TGAAGATCGT TTGCTGGCCA CACTTTTGCA CAACCTCATC 
TCCTACATGC TGCTGATGAA GGTAAATAAG AATGACATCC GGAAGAAGGT GAGGCGCCTA 
ATGGGAAAGT CCCATATTGG GCTTGTGTAC AGCCAGCAAA TCAATGAAGT GCTTGATCAG 
CTGGTGAACC TGAATGGACG TGATCTCTCT ATCCGGTCCA GTGGCAGCCG GCACATGAAA 
AAGCAGACAT TTGTGGTACA TGCAGGGACA GACACAAATG GAGACATCTT CTTCATGGAG 
GTGTGTGATG ACTGTGTGGT GTTACGTAGT AACATCGGAA CGGTGTATGA GCGCTGGTGG 
TATGAGAAGC TCATCAACAT GACCTACTGT CCCAAGACCA AGGTGTTGTG CTTGTGGCGT 
AGAAATGGCT CTGAGACTCA GCTCAACAAA TTCTATACTA AGAAGTGTCG GGAGCTGTAC 
TACTGTGTGA AGGACAGCAT GGAGCGGGCT GCTGCCCGGC AGCAAAGCAT CAAACCTGGC 
CCTGAACTGG GTGGCGAGTT CCCTGTGCAG GACATGAAGA CTGGTGAGGG TGGCTTGCTG 
CAGGTCACCC TAGAAGGGAT CAATCTCAAG TTCATGCACA ACCAGTTCCT GAAATTAAAG 
AAGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_855872.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_860965.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X22, mRNA.

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CloneID	OCe20074
Clone ID Related Accession (Same CDS sequence)	XM_855872.5 , XM_855872.4
Accession Version	XM_855872.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_855872.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG 
CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG 
AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG 
ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT 
GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA 
CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC 
ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA 
CCCCGGCCCG TCTCGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_860965.1
CDS	234..4874
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_855872.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_855872.5

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG 
CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG 
AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG 
ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT 
GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA 
CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC 
ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA 
CCCCGGCCCG TCTCGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_855872.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_860965.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OCe20074
Clone ID Related Accession (Same CDS sequence)	XM_855872.5 , XM_855872.4
Accession Version	XM_855872.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X19
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_855872.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG 
CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG 
AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG 
ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT 
GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA 
CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC 
ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA 
CCCCGGCCCG TCTCGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_860965.1
CDS	254..4894
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_855872.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_855872.4

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG 
CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG 
AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG 
ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT 
GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA 
CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC 
ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGG TTTTCATAGA GCTGAATCAC 
ATTAAAAAGT GCAATACAGT CCGAGGCGTC TTTGTCCTGG AGGAATTTGT TCCTGAAATT 
AAAGAAGTGG TGAGCCACAA GTACAAGACA CCAATGGCCC ATGAGATCTG CTACTCTGTG 
TTGTGTCTCT TCTCGTATGT GGCTGCAGTT CGTAGCAGTG AGGAAGATCT CAGGACCCCA 
CCCCGGCCCG TCTCGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005631249.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631306.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X24, mRNA.

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CloneID	OCe20075
Clone ID Related Accession (Same CDS sequence)	XM_005631249.2 , XM_005631249.3
Accession Version	XM_005631249.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X21
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631249.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT GAGTAATAGT 
TCTGGAGAGA CCCTTGGAGC AGATAGTGAC CTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGTGAAGGCA GCGCCCACTT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAATTCTG CTACCAGCAC CATCTTTGGG AAAGCCCATA GCTTGAAGCC AAGTGTAAAG 
GAGAAGCTGG TGGGCAGCCC AGTTCGCTCT TCCGAAGATG TAAGCCAACG GGTCTATCTG 
TACGAGGGAC TCCTAGGCAA AGAACGTTCT ACTTTGTGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTG GGAGAACATG ACCGGAAGCG CCTAGAGGAT 
GATGAAGATC GTTTGCTGGC CACACTTTTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TAATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAA GTGCTTGATC AGCTGGTGAA CCTGAATGGA 
CGTGATCTCT CTATCCGGTC CAGTGGCAGC CGGCACATGA AAAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGACACAAA TGGAGACATC TTCTTCATGG AGGTGTGTGA TGACTGTGTG 
GTGTTACGTA GTAACATCGG AACGGTGTAT GAGCGCTGGT GGTATGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC CAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACT 
CAGCTCAACA AATTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGGG CTGCTGCCCG GCAGCAAAGC ATCAAACCTG GCCCTGAACT GGGTGGCGAG 
TTCCCTGTGC AGGACATGAA GACTGGTGAG GGTGGCTTGC TGCAGGTCAC CCTAGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC 
TCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631306.1
CDS	254..4882
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005631249.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631249.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT GAGTAATAGT 
TCTGGAGAGA CCCTTGGAGC AGATAGTGAC CTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGTGAAGGCA GCGCCCACTT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAATTCTG CTACCAGCAC CATCTTTGGG AAAGCCCATA GCTTGAAGCC AAGTGTAAAG 
GAGAAGCTGG TGGGCAGCCC AGTTCGCTCT TCCGAAGATG TAAGCCAACG GGTCTATCTG 
TACGAGGGAC TCCTAGGCAA AGAACGTTCT ACTTTGTGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTG GGAGAACATG ACCGGAAGCG CCTAGAGGAT 
GATGAAGATC GTTTGCTGGC CACACTTTTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TAATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAA GTGCTTGATC AGCTGGTGAA CCTGAATGGA 
CGTGATCTCT CTATCCGGTC CAGTGGCAGC CGGCACATGA AAAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGACACAAA TGGAGACATC TTCTTCATGG AGGTGTGTGA TGACTGTGTG 
GTGTTACGTA GTAACATCGG AACGGTGTAT GAGCGCTGGT GGTATGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC CAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACT 
CAGCTCAACA AATTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGGG CTGCTGCCCG GCAGCAAAGC ATCAAACCTG GCCCTGAACT GGGTGGCGAG 
TTCCCTGTGC AGGACATGAA GACTGGTGAG GGTGGCTTGC TGCAGGTCAC CCTAGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC 
TCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005631249.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631306.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X24, mRNA.

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CloneID	OCe20075
Clone ID Related Accession (Same CDS sequence)	XM_005631249.2 , XM_005631249.3
Accession Version	XM_005631249.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631249.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT GAGTAATAGT 
TCTGGAGAGA CCCTTGGAGC AGATAGTGAC CTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGTGAAGGCA GCGCCCACTT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAATTCTG CTACCAGCAC CATCTTTGGG AAAGCCCATA GCTTGAAGCC AAGTGTAAAG 
GAGAAGCTGG TGGGCAGCCC AGTTCGCTCT TCCGAAGATG TAAGCCAACG GGTCTATCTG 
TACGAGGGAC TCCTAGGCAA AGAACGTTCT ACTTTGTGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTG GGAGAACATG ACCGGAAGCG CCTAGAGGAT 
GATGAAGATC GTTTGCTGGC CACACTTTTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TAATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAA GTGCTTGATC AGCTGGTGAA CCTGAATGGA 
CGTGATCTCT CTATCCGGTC CAGTGGCAGC CGGCACATGA AAAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGACACAAA TGGAGACATC TTCTTCATGG AGGTGTGTGA TGACTGTGTG 
GTGTTACGTA GTAACATCGG AACGGTGTAT GAGCGCTGGT GGTATGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC CAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACT 
CAGCTCAACA AATTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGGG CTGCTGCCCG GCAGCAAAGC ATCAAACCTG GCCCTGAACT GGGTGGCGAG 
TTCCCTGTGC AGGACATGAA GACTGGTGAG GGTGGCTTGC TGCAGGTCAC CCTAGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC 
TCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631306.1
CDS	234..4862
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005631249.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631249.3

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT GAGTAATAGT 
TCTGGAGAGA CCCTTGGAGC AGATAGTGAC CTGAGCAGCA ATGCAGGTGA TGGACCAGGT 
GGTGAAGGCA GCGCCCACTT GGCAAGCTCT CGGGGCACTT TGTCTGATAG TGAAATTGAG 
ACCAATTCTG CTACCAGCAC CATCTTTGGG AAAGCCCATA GCTTGAAGCC AAGTGTAAAG 
GAGAAGCTGG TGGGCAGCCC AGTTCGCTCT TCCGAAGATG TAAGCCAACG GGTCTATCTG 
TACGAGGGAC TCCTAGGCAA AGAACGTTCT ACTTTGTGGG ACCAAATGCA GTTCTGGGAA 
GATGCATTCT TAGATGCTGT GATGTTGGAG AGAGAAGGGA TGGGTATGGA CCAGGGTCCC 
CAGGAAATGA TCGACAGGTA CCTGTCCCTG GGAGAACATG ACCGGAAGCG CCTAGAGGAT 
GATGAAGATC GTTTGCTGGC CACACTTTTG CACAACCTCA TCTCCTACAT GCTGCTGATG 
AAGGTAAATA AGAATGACAT CCGGAAGAAG GTGAGGCGCC TAATGGGAAA GTCCCATATT 
GGGCTTGTGT ACAGCCAGCA AATCAATGAA GTGCTTGATC AGCTGGTGAA CCTGAATGGA 
CGTGATCTCT CTATCCGGTC CAGTGGCAGC CGGCACATGA AAAAGCAGAC ATTTGTGGTA 
CATGCAGGGA CAGACACAAA TGGAGACATC TTCTTCATGG AGGTGTGTGA TGACTGTGTG 
GTGTTACGTA GTAACATCGG AACGGTGTAT GAGCGCTGGT GGTATGAGAA GCTCATCAAC 
ATGACCTACT GTCCCAAGAC CAAGGTGTTG TGCTTGTGGC GTAGAAATGG CTCTGAGACT 
CAGCTCAACA AATTCTATAC TAAGAAGTGT CGGGAGCTGT ACTACTGTGT GAAGGACAGC 
ATGGAGCGGG CTGCTGCCCG GCAGCAAAGC ATCAAACCTG GCCCTGAACT GGGTGGCGAG 
TTCCCTGTGC AGGACATGAA GACTGGTGAG GGTGGCTTGC TGCAGGTCAC CCTAGAAGGG 
ATCAATCTCA AGTTCATGCA CAACCAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC 
AATACAGTCC GAGGCGTCTT TGTCCTGGAG GAATTTGTTC CTGAAATTAA AGAAGTGGTG 
AGCCACAAGT ACAAGACACC AATGGCCCAT GAGATCTGCT ACTCTGTGTT GTGTCTCTTC 
TCGTATGTGG CTGCAGTTCG TAGCAGTGAG GAAGATCTCA GGACCCCACC CCGGCCCGTC 
TCGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005631250.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631307.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

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CloneID	OCe20076
Clone ID Related Accession (Same CDS sequence)	XM_005631250.3 , XM_005631250.2
Accession Version	XM_005631250.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631250.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
AGTAATAGTT CTGGAGAGAC CCTTGGAGCA GATAGTGACC TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GTGAAGGCAG CGCCCACTTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAATTCTGC TACCAGCACC ATCTTTGGGA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CCGAAGATGT AAGCCAACGG 
GTCTATCTGT ACGAGGGACT CCTAGGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC 
CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC 
CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT 
GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG 
GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC 
CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCATG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC TGCAGTTCGT AGCAGTGAGG AAGATCTCAG GACCCCACCC 
CGGCCCGTCT CGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631307.1
CDS	234..4871
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005631250.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631250.3

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
AGTAATAGTT CTGGAGAGAC CCTTGGAGCA GATAGTGACC TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GTGAAGGCAG CGCCCACTTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAATTCTGC TACCAGCACC ATCTTTGGGA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CCGAAGATGT AAGCCAACGG 
GTCTATCTGT ACGAGGGACT CCTAGGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC 
CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC 
CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT 
GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG 
GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC 
CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCATG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC TGCAGTTCGT AGCAGTGAGG AAGATCTCAG GACCCCACCC 
CGGCCCGTCT CGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_005631250.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631307.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OCe20076
Clone ID Related Accession (Same CDS sequence)	XM_005631250.3 , XM_005631250.2
Accession Version	XM_005631250.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X20
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
AGTAATAGTT CTGGAGAGAC CCTTGGAGCA GATAGTGACC TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GTGAAGGCAG CGCCCACTTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAATTCTGC TACCAGCACC ATCTTTGGGA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CCGAAGATGT AAGCCAACGG 
GTCTATCTGT ACGAGGGACT CCTAGGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC 
CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC 
CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT 
GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG 
GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC 
CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCATG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC TGCAGTTCGT AGCAGTGAGG AAGATCTCAG GACCCCACCC 
CGGCCCGTCT CGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631307.1
CDS	255..4892
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEEDLRTPPRPVSS

Target ORF information:

RefSeq Version	XM_005631250.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631250.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
AGTAATAGTT CTGGAGAGAC CCTTGGAGCA GATAGTGACC TGAGCAGCAA TGCAGGTGAT 
GGACCAGGTG GTGAAGGCAG CGCCCACTTG GCAAGCTCTC GGGGCACTTT GTCTGATAGT 
GAAATTGAGA CCAATTCTGC TACCAGCACC ATCTTTGGGA AAGCCCATAG CTTGAAGCCA 
AGTGTAAAGG AGAAGCTGGT GGGCAGCCCA GTTCGCTCTT CCGAAGATGT AAGCCAACGG 
GTCTATCTGT ACGAGGGACT CCTAGGCAAA GAACGTTCTA CTTTGTGGGA CCAAATGCAG 
TTCTGGGAAG ATGCATTCTT AGATGCTGTG ATGTTGGAGA GAGAAGGGAT GGGTATGGAC 
CAGGGTCCCC AGGAAATGAT CGACAGGTAC CTGTCCCTGG GAGAACATGA CCGGAAGCGC 
CTAGAGGATG ATGAAGATCG TTTGCTGGCC ACACTTTTGC ACAACCTCAT CTCCTACATG 
CTGCTGATGA AGGTAAATAA GAATGACATC CGGAAGAAGG TGAGGCGCCT AATGGGAAAG 
TCCCATATTG GGCTTGTGTA CAGCCAGCAA ATCAATGAAG TGCTTGATCA GCTGGTGAAC 
CTGAATGGAC GTGATCTCTC TATCCGGTCC AGTGGCAGCC GGCACATGAA AAAGCAGACA 
TTTGTGGTAC ATGCAGGGAC AGACACAAAT GGAGACATCT TCTTCATGGA GGTGTGTGAT 
GACTGTGTGG TGTTACGTAG TAACATCGGA ACGGTGTATG AGCGCTGGTG GTATGAGAAG 
CTCATCAACA TGACCTACTG TCCCAAGACC AAGGTGTTGT GCTTGTGGCG TAGAAATGGC 
TCTGAGACTC AGCTCAACAA ATTCTATACT AAGAAGTGTC GGGAGCTGTA CTACTGTGTG 
AAGGACAGCA TGGAGCGGGC TGCTGCCCGG CAGCAAAGCA TCAAACCTGG CCCTGAACTG 
GGTGGCGAGT TCCCTGTGCA GGACATGAAG ACTGGTGAGG GTGGCTTGCT GCAGGTCACC 
CTAGAAGGGA TCAATCTCAA GTTCATGCAC AACCAGGTTT TCATAGAGCT GAATCACATT 
AAAAAGTGCA ATACAGTCCG AGGCGTCTTT GTCCTGGAGG AATTTGTTCC TGAAATTAAA 
GAAGTGGTGA GCCACAAGTA CAAGACACCA ATGGCCCATG AGATCTGCTA CTCTGTGTTG 
TGTCTCTTCT CGTATGTGGC TGCAGTTCGT AGCAGTGAGG AAGATCTCAG GACCCCACCC 
CGGCCCGTCT CGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121191.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976666.1 Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OCe20077
Clone ID Related Accession (Same CDS sequence)	XM_014121191.1 , XM_014121191.2
Accession Version	XM_014121191.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	MAP kinase-activating death domain protein isoform X22
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG 
CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG 
AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG 
ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT 
GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA 
CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC 
ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976666.1
CDS	254..4696
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_014121191.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris MAP-kinase activating death domain (MADD), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121191.1

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG 
CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG 
AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG 
ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT 
GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA 
CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC 
ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MADD ( XM_014121191.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976666.1 Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

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CloneID	OCe20077
Clone ID Related Accession (Same CDS sequence)	XM_014121191.1 , XM_014121191.2
Accession Version	XM_014121191.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	MAP kinase-activating death domain protein isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121191.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG 
CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG 
AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG 
ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT 
GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA 
CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC 
ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976666.1
CDS	234..4676
Translation	MVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHAEFPLPPDVVFFCQPEGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKDKGDGGAGSRGKEGPRATCAPEEIGTESSESGLSLQPPSADSAPPNVTQSPRGKPRAKTGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGIQRDTMWRIFTGSLLVEEKSSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALTFALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPESLELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAEALSVPPERDSDSDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQNQKESEEPGPDSENSQENLPLRSSSSTTASSSPSTVIHGANSEPADSTEVDDKAAVGVSKPPPTVPPSIGKSNVDRRQTEIGEGAQKMLRPNSLKLASDSDAESDSRASSPTSTVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRTVQSEDDARQDVIPDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAAGKGPKELDTRSLKEENFVASVGPEVIKPVFDLGETEEKKSQISADSGVSLTSGSQRTDTDSVIGVSPAVMIRSSSQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRGTLSDSEIETNSATSTIFGKAHSLKPSVKEKLVGSPVRSSEDVSQRVYLYEGLLGKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLVNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW

Target ORF information:

RefSeq Version	XM_014121191.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MAP kinase activating death domain (MADD), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121191.2

ORF Insert Sequence:

ATGGTGCAGA AGAAGAAGTT CTGTCCTCGG TTACTTGACT ATCTCGTGAT CGTAGGGGCC 
AGGCACCCAA GCAGTGATAG TGTGGCCCAG ACTCCTGAAT TATTACGGCG CTATCCACTG 
GAGGACCACG CTGAGTTTCC CCTGCCCCCA GATGTAGTGT TCTTCTGCCA ACCAGAGGGC 
TGTCTGAGTG TGCGGCAGCG GCGCATGAGC CTGCGGGATG ATACTTCTTT TGTCTTCACC 
CTCACTGACA AGGACACTGG AGTCACTCGT TATGGCATCT GTGTTAACTT CTACCGCTCC 
TTCCAGAAGC GTATGCCTAA GGACAAGGGG GATGGGGGGG CAGGGTCCCG TGGGAAGGAA 
GGACCCCGTG CTACTTGTGC CCCAGAAGAG ATTGGCACTG AGAGCTCAGA GAGTGGCTTG 
TCCCTGCAGC CTCCCAGTGC TGACTCTGCC CCCCCTAATG TGACTCAGTC TCCTCGGGGC 
AAACCCCGGG CCAAGACGGG AAGCCGCTCC CGCAATAGTA CTCTGACATC CTTGTGCGTG 
CTCAGCCACT ATCCCTTCTT CTCCACCTTC CGAGAATGTC TGTACACCCT CAAACGTCTG 
GTGGATTGCT GCAGTGAGCG GCTGCTGGGC AAGAAACTAG GCATCCCTCG AGGTATACAA 
AGGGACACTA TGTGGCGCAT CTTTACTGGA TCATTGCTAG TGGAGGAGAA GTCAAGTGCC 
CTTCTTCATG ACCTTCGAGA GATCGAGGCC TGGATCTATC GATTGCTACG CTCCCCAGTG 
CCTGTCTCTG GGCAGAAGCG AGTGGACATT GAGGTCCTAC CCCAGGAGCT CCAACAAGCT 
CTGACCTTTG CTCTTCCAGA CCCTTCTCGA TTCACCCTAG TGGATTTCCC ACTGCACCTT 
CCCTTGGAAC TTCTGGGTGT GGATGCCTGT CTCCAGGTGC TAACCTGCAT CCTGTTAGAG 
CACAAGGTGG TGCTACAGTC CCGAGACTAC AATGCCCTCT CCATGTCTGT GATGGCGTTT 
GTGGCAATGA TCTACCCACT GGAGTATATG TTTCCTGTAA TCCCACTGCT GCCCACCTGC 
ATGGCATCGG CAGAACAGCT GCTGTTAGCT CCAACTCCGT ACATCATCGG GGTCCCTGCC 
AGCTTCTTCC TCTACAAACT GGACTTCAAA ATGCCTGATG ATGTATGGCT GGTGGATCTG 
GACAGCAATA GGGTGATTGC CCCCACCAAT GCAGAAGTGC TACCAATCCT GCCAGAACCG 
GAATCATTGG AGCTGAAAAA GCATTTGAAG CAGGCCCTTG CCAGCATGAG TCTCAACACC 
CAGCCCATCC TCAATCTGGA GAAATTCCAT GAAGGCCAAG AGATCCCCCT TCTCTTGGGT 
AGGCCTTCTA ACGACTTGCA GTCCACACCT TCCACTGAAT TCAACCCACT CATCTATGGC 
AATGATGTAG ATTCTGTGGA TGTTGCAACC AGAGTGGCCA TGGTCCGTTT CTTCAACTCC 
CCCAACGTGC TGCAGGGCTT TCAGATGCAC ACACGTACCC TGCGTCTCTT CCCGCGGCCT 
GTGGTAGCTT TTCAAGCTGG CTCCTTTCTA GCCTCACGTC CCCGGCAGAC TCCTTTTGCT 
GAGAAACTGG CCAGGACACA GGCTGTGGAG TACTTTGGAG AATGGATCCT TAACCCCACC 
AACTATGCCT TCCAGCGAAT TCACAACAAC ATGTTTGATC CAGCCCTGAT TGGTGACAAG 
CCAAAGTGGT ATGCTCATCA GCTGCAGCCT ATCCATTATC GAGTCTATGA TAGCAATTCC 
CAGCTGGCGG AGGCACTGAG TGTGCCACCA GAGCGTGACT CTGACTCTGA CCCTACTGAT 
GACAGTGGCA GTGATAGTAT GGATTATGAT GACTCAAGCT CTTCCTACTC CTCCCTTGGT 
GACTTTGTCA GTGAAATGAT GAAATGTGAC ATCAATGGTG ATACTCCCAA TGTGGATCCA 
TTGACACACG CAGCCCTGGG GGATGCCAGT GAAGTGGAGA TTGATGAGCT GCAAAACCAG 
AAGGAATCAG AGGAACCTGG CCCAGACAGT GAGAACTCTC AGGAAAACCT GCCACTGCGC 
TCCAGCTCCA GCACCACTGC CAGCAGTAGC CCCAGCACTG TCATCCATGG AGCTAATTCT 
GAACCTGCCG ACTCAACGGA GGTGGACGAT AAGGCAGCAG TAGGTGTCTC CAAGCCCCCC 
CCCACAGTGC CTCCCAGCAT TGGCAAGTCG AACGTGGACA GGCGTCAGAC AGAAATTGGA 
GAGGGGGCTC AAAAGATGCT GCGGCCCAAC AGCTTGAAAC TGGCAAGCGA CTCAGACGCA 
GAGTCAGACT CTCGAGCGAG CTCTCCCACC TCCACCGTCT CCAACACCAG CACCGAGGGC 
TTCGGGGGCA TCATGTCTTT TGCCAGCAGC CTGTATCGAA ACCACAGTAC AAGCTTCAGT 
CTTTCAAACC TCACACTGCC GACCAAAGGT GCCCGAGAGA AGACGACCCC CTTCCCCAGT 
CTGAAAGGAA ACAGGAGGGC CTTAGTGGAC CAGAAGTCAT CTGTTATTAA ACACAGCCCA 
ACAGTGAAAA GAGAGCCTCC ATCACCTCAG GGTCGATCCA GCAATTCTAG TGAGAACCAG 
CAGTTCCTGA AGGAAGTGGT ACACAGCGTG CTGGATGGCC AGGGTGTTGG CTGGCTCAAC 
ATGAAAAAGG TGCGTCGGCT GCTGGAGAGT GAGCAGCTGC GAGTCTTTGT CCTGAGCAAG 
CTGAACCGAA CTGTGCAGTC AGAAGATGAT GCCCGGCAGG ACGTCATCCC CGATGTGGAG 
ATCAGCAGAA AGGTTTACAA GGGGATGTTA GACCTGCTCA AGTGCACAGT ACTCAGCCTG 
GAGCAGTCCT ATGCCCACGC AGGTCTGGGT GGCATGGCCA GCATCTTTGG GCTTCTGGAG 
ATTGCCCAGA CCCACTACTA TAGTAAAGAA CCAGACAAGC GGAAGAGAAG TCCAACAGAG 
AGTGTAAATA CACCAGTTGG CAAGGATCCT GGCCTGGCTG GGCGGGGGGA CCCAAAAGCT 
ATGGCACAGC TGAGAGTTCC CCAGCTGGGC CCTCGGGCGC CAAGTGCTGC AGGAAAGGGT 
CCCAAAGAAC TAGACACCAG AAGTTTAAAG GAAGAGAATT TTGTAGCATC TGTTGGGCCT 
GAAGTCATCA AACCCGTCTT TGACCTTGGT GAGACAGAGG AGAAAAAGTC CCAGATCAGC 
GCAGACAGTG GTGTGAGCCT GACATCTGGT TCCCAGAGGA CTGATACAGA CTCTGTCATT 
GGTGTGAGTC CAGCTGTTAT GATCCGAAGC TCAAGTCAGG ACTCTGAAGT TAGCACCGTG 
GTGAGTAATA GTTCTGGAGA GACCCTTGGA GCAGATAGTG ACCTGAGCAG CAATGCAGGT 
GATGGACCAG GTGGTGAAGG CAGCGCCCAC TTGGCAAGCT CTCGGGGCAC TTTGTCTGAT 
AGTGAAATTG AGACCAATTC TGCTACCAGC ACCATCTTTG GGAAAGCCCA TAGCTTGAAG 
CCAAGTGTAA AGGAGAAGCT GGTGGGCAGC CCAGTTCGCT CTTCCGAAGA TGTAAGCCAA 
CGGGTCTATC TGTACGAGGG ACTCCTAGGC AAAGAACGTT CTACTTTGTG GGACCAAATG 
CAGTTCTGGG AAGATGCATT CTTAGATGCT GTGATGTTGG AGAGAGAAGG GATGGGTATG 
GACCAGGGTC CCCAGGAAAT GATCGACAGG TACCTGTCCC TGGGAGAACA TGACCGGAAG 
CGCCTAGAGG ATGATGAAGA TCGTTTGCTG GCCACACTTT TGCACAACCT CATCTCCTAC 
ATGCTGCTGA TGAAGGTAAA TAAGAATGAC ATCCGGAAGA AGGTGAGGCG CCTAATGGGA 
AAGTCCCATA TTGGGCTTGT GTACAGCCAG CAAATCAATG AAGTGCTTGA TCAGCTGGTG 
AACCTGAATG GACGTGATCT CTCTATCCGG TCCAGTGGCA GCCGGCACAT GAAAAAGCAG 
ACATTTGTGG TACATGCAGG GACAGACACA AATGGAGACA TCTTCTTCAT GGAGGTGTGT 
GATGACTGTG TGGTGTTACG TAGTAACATC GGAACGGTGT ATGAGCGCTG GTGGTATGAG 
AAGCTCATCA ACATGACCTA CTGTCCCAAG ACCAAGGTGT TGTGCTTGTG GCGTAGAAAT 
GGCTCTGAGA CTCAGCTCAA CAAATTCTAT ACTAAGAAGT GTCGGGAGCT GTACTACTGT 
GTGAAGGACA GCATGGAGCG GGCTGCTGCC CGGCAGCAAA GCATCAAACC TGGCCCTGAA 
CTGGGTGGCG AGTTCCCTGT GCAGGACATG AAGACTGGTG AGGGTGGCTT GCTGCAGGTC 
ACCCTAGAAG GGATCAATCT CAAGTTCATG CACAACCAGT TCCTGAAATT AAAGAAGTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

MADD cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: