RNF20 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF20
Entrez Gene ID	474788
Full Name	ring finger protein 20
General protein information	Preferred Names ring finger protein 20 Names E3 ubiquitin-protein ligase BRE1A
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005626880.2	XP_005626937.1	E3 ubiquitin-protein ligase BRE1A
XM_022425660.1	XP_022281368.1	E3 ubiquitin-protein ligase BRE1A isoform X1
XM_005626880.3	XP_005626937.1	E3 ubiquitin-protein ligase BRE1A isoform X1
XM_532018.4	XP_532018.3	E3 ubiquitin-protein ligase BRE1A
XM_532018.5	XP_532018.3	E3 ubiquitin-protein ligase BRE1A isoform X1
XM_022425661.1	XP_022281369.1	E3 ubiquitin-protein ligase BRE1A isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RNF20	XP_005626937.1
Rattus norvegicus (Norway rat)	Rnf20	NP_001101399.2
Xenopus tropicalis (tropical clawed frog)	rnf20	XP_002941911.1
Mus musculus (house mouse)	Rnf20	NP_001156735.1
Gallus gallus (chicken)	RNF20	NP_001026605.1
Pan troglodytes (chimpanzee)	RNF20	XP_520159.2
	RNF20	XP_001110807.1
Bos taurus (cattle)	RNF20	NP_001075056.1
Danio rerio (zebrafish)	rnf20	NP_001243104.1
Homo sapiens (human)	RNF20	NP_062538.5

Gene Ontology

The following RNF20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe14708	XM_005626880.3 Latest version!	Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe14708	XM_532018.5 Latest version!	Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe14708	XM_022425660.1 Latest version!	Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe292198	XM_022425661.1 Latest version!	Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OCe14708	XM_005626880.2	Canis lupus familiaris ring finger protein 20, E3 ubiquitin protein ligase (RNF20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe14708	XM_532018.4	Canis lupus familiaris ring finger protein 20, E3 ubiquitin protein ligase (RNF20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF20 ( XM_005626880.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626937.1 Canis lupus familiaris ring finger protein 20, E3 ubiquitin protein ligase (RNF20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe14708
Clone ID Related Accession (Same CDS sequence)	XM_005626880.2 , XM_022425660.1 , XM_005626880.3 , XM_532018.4 , XM_532018.5
Accession Version	XM_005626880.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase BRE1A
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC 
TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA 
ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG 
CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC 
CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC 
TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG 
CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG 
CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG 
GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC 
GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT 
TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC 
AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA 
TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG 
CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT 
GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG 
CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT 
GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT 
AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA 
GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA 
AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA 
AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA 
CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG 
AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG 
AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA 
GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA 
CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG 
ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT 
AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626937.1
CDS	285..3212
Translation	MSGIGNKRAAGEPGTSMPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSCKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDLEEVTTQNEKLKVELRSAVEEAVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKQDPEDVPAQSSASKASQEEVNEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDSAKDKEKGKHDDGRKKEAEVIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	XM_005626880.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ring finger protein 20, E3 ubiquitin protein ligase (RNF20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626880.2

ORF Insert Sequence:

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC 
TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA 
ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG 
CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC 
CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC 
TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG 
CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG 
CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG 
GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC 
GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT 
TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC 
AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA 
TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG 
CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT 
GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG 
CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT 
GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT 
AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA 
GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA 
AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA 
AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA 
CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG 
AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG 
AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA 
GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA 
CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG 
ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT 
AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF20 ( XM_022425660.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281368.1 Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe14708
Clone ID Related Accession (Same CDS sequence)	XM_005626880.2 , XM_022425660.1 , XM_005626880.3 , XM_532018.4 , XM_532018.5
Accession Version	XM_022425660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase BRE1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC 
TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA 
ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG 
CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC 
CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC 
TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG 
CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG 
CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG 
GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC 
GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT 
TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC 
AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA 
TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG 
CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT 
GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG 
CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT 
GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT 
AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA 
GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA 
AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA 
AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA 
CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG 
AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG 
AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA 
GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA 
CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG 
ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT 
AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281368.1
CDS	152..3079
Translation	MSGIGNKRAAGEPGTSMPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSCKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDLEEVTTQNEKLKVELRSAVEEAVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKQDPEDVPAQSSASKASQEEVNEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDSAKDKEKGKHDDGRKKEAEVIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	XM_022425660.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022425660.1

ORF Insert Sequence:

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC 
TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA 
ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG 
CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC 
CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC 
TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG 
CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG 
CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG 
GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC 
GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT 
TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC 
AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA 
TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG 
CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT 
GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG 
CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT 
GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT 
AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA 
GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA 
AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA 
AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA 
CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG 
AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG 
AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA 
GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA 
CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG 
ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT 
AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF20 ( XM_005626880.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626937.1 Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe14708
Clone ID Related Accession (Same CDS sequence)	XM_005626880.2 , XM_022425660.1 , XM_005626880.3 , XM_532018.4 , XM_532018.5
Accession Version	XM_005626880.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase BRE1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC 
TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA 
ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG 
CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC 
CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC 
TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG 
CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG 
CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG 
GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC 
GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT 
TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC 
AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA 
TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG 
CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT 
GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG 
CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT 
GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT 
AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA 
GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA 
AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA 
AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA 
CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG 
AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG 
AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA 
GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA 
CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG 
ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT 
AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626937.1
CDS	178..3105
Translation	MSGIGNKRAAGEPGTSMPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSCKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDLEEVTTQNEKLKVELRSAVEEAVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKQDPEDVPAQSSASKASQEEVNEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDSAKDKEKGKHDDGRKKEAEVIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	XM_005626880.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626880.3

ORF Insert Sequence:

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC 
TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA 
ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG 
CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC 
CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC 
TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG 
CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG 
CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG 
GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC 
GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT 
TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC 
AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA 
TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG 
CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT 
GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG 
CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT 
GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT 
AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA 
GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA 
AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA 
AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA 
CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG 
AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG 
AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA 
GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA 
CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG 
ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT 
AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF20 ( XM_532018.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_532018.3 Canis lupus familiaris ring finger protein 20, E3 ubiquitin protein ligase (RNF20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe14708
Clone ID Related Accession (Same CDS sequence)	XM_005626880.2 , XM_022425660.1 , XM_005626880.3 , XM_532018.4 , XM_532018.5
Accession Version	XM_532018.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase BRE1A
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_532018.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC 
TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA 
ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG 
CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC 
CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC 
TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG 
CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG 
CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG 
GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC 
GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT 
TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC 
AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA 
TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG 
CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT 
GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG 
CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT 
GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT 
AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA 
GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA 
AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA 
AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA 
CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG 
AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG 
AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA 
GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA 
CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG 
ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT 
AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_532018.3
CDS	183..3110
Translation	MSGIGNKRAAGEPGTSMPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSCKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDLEEVTTQNEKLKVELRSAVEEAVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKQDPEDVPAQSSASKASQEEVNEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDSAKDKEKGKHDDGRKKEAEVIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	XM_532018.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ring finger protein 20, E3 ubiquitin protein ligase (RNF20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_532018.4

ORF Insert Sequence:

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC 
TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA 
ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG 
CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC 
CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC 
TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG 
CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG 
CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG 
GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC 
GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT 
TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC 
AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA 
TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG 
CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT 
GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG 
CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT 
GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT 
AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA 
GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA 
AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA 
AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA 
CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG 
AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG 
AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA 
GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA 
CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG 
ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT 
AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF20 ( XM_532018.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_532018.3 Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe14708
Clone ID Related Accession (Same CDS sequence)	XM_005626880.2 , XM_022425660.1 , XM_005626880.3 , XM_532018.4 , XM_532018.5
Accession Version	XM_532018.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase BRE1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_532018.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC 
TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA 
ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG 
CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC 
CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC 
TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG 
CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG 
CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG 
GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC 
GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT 
TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC 
AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA 
TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG 
CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT 
GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG 
CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT 
GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT 
AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA 
GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA 
AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA 
AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA 
CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG 
AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG 
AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA 
GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA 
CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG 
ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT 
AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_532018.3
CDS	180..3107
Translation	MSGIGNKRAAGEPGTSMPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSCKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDLEEVTTQNEKLKVELRSAVEEAVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKQDPEDVPAQSSASKASQEEVNEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDSAKDKEKGKHDDGRKKEAEVIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	XM_532018.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_532018.5

ORF Insert Sequence:

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAGCCTG GCACATCCAT GCCTCCGGAG 
AAGAAGACAG CCGTTGAAGA TTCAGGGACC ACAGTGGAGA CAATTAAGCT TGGAGGTGTC 
TCCTCCACGG AGGAGCTAGA CATTCGAACA CTGCAGACCA AAAATCGCAA GCTGGCAGAA 
ATGCTGGATC AACGGCAGGC CATCGAAGAT GAACTCCGTG AGCACATTGA AAAACTGGAG 
CGAAGACAGG CCACTGATGA TGCCTCACTG CTGATCGTCA ACCGGTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGG TACGATCTGG AGCAGGGTTT GGGAGATCTC 
CTCACAGAAA GGAAAGCCCT TGTCGTGCCC GAACCAGAGC CGGACTCTGA TAGCAATCAG 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAAATG GAATCTCAGC TGCAGGAGCG AGTGGAGTCC 
TCCCGCCGAG CTGTGTCCCA GATCGTGACT GTCTATGATA AATTACAAGA GAAAGTGGAG 
CTGTTATCAT GCAAACTAAA TAGTGGAGAT AATCTGATAG TGGAAGAGGC GGTGCAGGAG 
CTGAATTCCT TCCTTGCACA GGAGAATATG AGGCTACAGG AATTGACAGA CCTCCTTCAG 
GAGAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTAC AGAGTAAAGT GGAGACAGCC 
GAGTCGCGAG TGTCTGTCCT AGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAACG ACTCAACCGA CACTTAGCAG AAGTTCTTGA ACGGGTGAAT 
TCGAAAGGTT ATAAGGTCTA TGGAGCCGGG AGCAGTCTCT ATGGTGGCAC AATCACTATC 
AATGCCCGGA AGTTTGAGGA AATGAATGCA GAGCTGGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTGG AGGAGGTCAC TACGCAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGCGCG GTGGAGGAGG CAGTTAAGGA GACTCCGGAA 
TACCGCTGTA TGCAGTCACA GTTCTCCGTC CTGTACAATG AGAGCCTGCA GTTGAAAGCG 
CACTTGGATG AGGCTCGGAC TCTGCTTCAT GGCACCAGGG GGACCCACCA GCGCCAAGTT 
GAGCTTATTG AGCGAGATGA AGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTGATCCAG 
CTGGAAGATA CTCTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA GCAAGCAGGC CCCATAAACC GGGAGATGCG TCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTAAGATA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGTCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGATCCAAAG GATGAGCCTG CAGAGCTGAA GCAAGATCCT 
GAGGACGTAC CTGCCCAGTC CTCCGCATCA AAGGCATCTC AGGAGGAGGT CAATGAAATT 
AAGTCCAAAC GGGACGAGGA AGAACGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA AAAGGAGAGG GAACGAGAGA AGCAGAAACT GAAAGAGTCA 
GAAAAAGAAA GAGACTCTGC TAAGGATAAA GAGAAAGGGA AACATGACGA TGGAAGGAAA 
AAGGAAGCAG AAGTTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA AGAGAGCCAA 
AAGGAGATGA AACTATTGCT AGATATGTAC CGCTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCA AAGGCAGAGT TGGAAGATTT AAGGCAAAGA 
CTCAAGGATC TAGAGGATAA GGAGAAAAAG GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT AGAAGAGCAG ATAGAGTACC TGCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AGGAAGAGGC ACTCCTCTCA GAGATGGATG TTACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTAATG CAGCAGTTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCTGAGCG TATCAAGTCC AATCAGATCC ACAAGTTGCT CAAAGAAGAG 
AAGGAGGAGC TGGCTGACCA AGTTTTGACT CTAAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTAAGGA AATTGGAAGA GAAGGAGCAT CTGTTACAAA GCAACATTGG CACAGGGGAG 
AAGGAACTGG GTCTTAGGAC ACAAGCCTTA GAGATGAATA AACGCAAGGC GATGGAAGCA 
GCCCAGCTGG CAGATGACCT CAAAGCGCAA CTGGAATTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAATAGTGTC ACCAAAGAAA AGGATATGTT TAATTTCAAA 
CGAGCTCAGG AGGACATCTC TAGACTCCGA AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTGCCCA AATGTGATGA GATCCTGATG GAGGAGATTA AGGATTACAA GGCACGCCTG 
ACCTGTCCAT GTTGCAATAT GCGTAAAAAG GATGCTGTAC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTAAA GACACGCTAT GACACCCGTC AGCGCAAATG TCCCAAGTGT 
AATGCTGCGT TTGGTGCCAA TGATTTCCAC CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF20 ( XM_022425661.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281369.1 Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe292198
Clone ID Related Accession (Same CDS sequence)	XM_022425661.1
Accession Version	XM_022425661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase BRE1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGG AAAGTGTTGG AGGTTTGAAG AGACAGGAGA AGGAGGAGCT AGACATTCGA 
ACACTGCAGA CCAAAAATCG CAAGCTGGCA GAAATGCTGG ATCAACGGCA GGCCATCGAA 
GATGAACTCC GTGAGCACAT TGAAAAACTG GAGCGAAGAC AGGCCACTGA TGATGCCTCA 
CTGCTGATCG TCAACCGGTA CTGGAGTCAG TTTGATGAAA ACATCCGTAT CATCCTTAAA 
CGGTACGATC TGGAGCAGGG TTTGGGAGAT CTCCTCACAG AAAGGAAAGC CCTTGTCGTG 
CCCGAACCAG AGCCGGACTC TGATAGCAAT CAGGAACGGA AAGATGACCG AGAGAGAGGG 
GAAGGGCAAG AGCCAGCTTT CTCTTTCCTT GCTACTTTGG CCAGCAGTTC CAGTGAAGAA 
ATGGAATCTC AGCTGCAGGA GCGAGTGGAG TCCTCCCGCC GAGCTGTGTC CCAGATCGTG 
ACTGTCTATG ATAAATTACA AGAGAAAGTG GAGCTGTTAT CATGCAAACT AAATAGTGGA 
GATAATCTGA TAGTGGAAGA GGCGGTGCAG GAGCTGAATT CCTTCCTTGC ACAGGAGAAT 
ATGAGGCTAC AGGAATTGAC AGACCTCCTT CAGGAGAAGC ATCGCACCAT GTCTCAGGAG 
TTCTCCAAGT TACAGAGTAA AGTGGAGACA GCCGAGTCGC GAGTGTCTGT CCTAGAGTCC 
ATGATCGATG ACCTGCAGTG GGATATTGAC AAAATTCGAA AGAGGGAACA ACGACTCAAC 
CGACACTTAG CAGAAGTTCT TGAACGGGTG AATTCGAAAG GTTATAAGGT CTATGGAGCC 
GGGAGCAGTC TCTATGGTGG CACAATCACT ATCAATGCCC GGAAGTTTGA GGAAATGAAT 
GCAGAGCTGG AGGAGAACAA AGAGTTGGCT CAGAACCGTC ACTGTGAGCT GGAGAAACTT 
CGGCAAGACT TGGAGGAGGT CACTACGCAA AATGAAAAGC TGAAGGTGGA ATTGCGGAGC 
GCGGTGGAGG AGGCAGTTAA GGAGACTCCG GAATACCGCT GTATGCAGTC ACAGTTCTCC 
GTCCTGTACA ATGAGAGCCT GCAGTTGAAA GCGCACTTGG ATGAGGCTCG GACTCTGCTT 
CATGGCACCA GGGGGACCCA CCAGCGCCAA GTTGAGCTTA TTGAGCGAGA TGAAGTTAGT 
CTTCATAAGA AGCTGAGGAC TGAAGTGATC CAGCTGGAAG ATACTCTGGC CCAGGTCCGC 
AAGGAGTATG AGATGCTGAG GATAGAATTT GAGCAGACCC TTGCTGCCAA TGAGCAAGCA 
GGCCCCATAA ACCGGGAGAT GCGTCACCTC ATCAGTAGCC TCCAGAATCA CAATCACCAG 
CTGAAAGGGG AGGTCCTAAG ATATAAGCGG AAACTGAGAG AAGCCCAGTC TGACCTGAAC 
AAGACACGTC TGCGCAGTGG CAGTGCCCTC CTGCAGTCTC AGTCCAGTAC TGAGGATCCA 
AAGGATGAGC CTGCAGAGCT GAAGCAAGAT CCTGAGGACG TACCTGCCCA GTCCTCCGCA 
TCAAAGGCAT CTCAGGAGGA GGTCAATGAA ATTAAGTCCA AACGGGACGA GGAAGAACGA 
GAACGAGAAA GGAGGGAGAA AGAGAGGGAG CGAGAAAGAG AGCGGGAGAA GGAAAAGGAG 
AGGGAACGAG AGAAGCAGAA ACTGAAAGAG TCAGAAAAAG AAAGAGACTC TGCTAAGGAT 
AAAGAGAAAG GGAAACATGA CGATGGAAGG AAAAAGGAAG CAGAAGTTAT CAAACAATTG 
AAGATTGAAC TCAAGAAGGC ACAAGAGAGC CAAAAGGAGA TGAAACTATT GCTAGATATG 
TACCGCTCTG CCCCAAAGGA ACAGAGAGAC AAAGTTCAGC TGATGGCAGC TGAGAAGAAG 
TCAAAGGCAG AGTTGGAAGA TTTAAGGCAA AGACTCAAGG ATCTAGAGGA TAAGGAGAAA 
AAGGAGAACA AGAAAATGGC TGATGAGGAT GCCTTGAGGA AGATCCGGGC AGTAGAAGAG 
CAGATAGAGT ACCTGCAGAA GAAGCTGGCC ATGGCCAAGC AGGAGGAAGA GGCACTCCTC 
TCAGAGATGG ATGTTACAGG CCAGGCCTTT GAAGACATGC AGGAGCAAAA TATCCGTTTA 
ATGCAGCAGT TGCGGGAGAA GGATGATGCA AATTTCAAGC TCATGTCTGA GCGTATCAAG 
TCCAATCAGA TCCACAAGTT GCTCAAAGAA GAGAAGGAGG AGCTGGCTGA CCAAGTTTTG 
ACTCTAAAGA CTCAGGTTGA TGCCCAGTTA CAGGTAGTAA GGAAATTGGA AGAGAAGGAG 
CATCTGTTAC AAAGCAACAT TGGCACAGGG GAGAAGGAAC TGGGTCTTAG GACACAAGCC 
TTAGAGATGA ATAAACGCAA GGCGATGGAA GCAGCCCAGC TGGCAGATGA CCTCAAAGCG 
CAACTGGAAT TGGCTCAGAA GAAGCTACAT GATTTTCAGG ATGAGATCGT GGAGAATAGT 
GTCACCAAAG AAAAGGATAT GTTTAATTTC AAACGAGCTC AGGAGGACAT CTCTAGACTC 
CGAAGGAAGC TGGAGACCAC AAAGAAACCA GACAATGTGC CCAAATGTGA TGAGATCCTG 
ATGGAGGAGA TTAAGGATTA CAAGGCACGC CTGACCTGTC CATGTTGCAA TATGCGTAAA 
AAGGATGCTG TACTTACCAA GTGTTTTCAT GTTTTCTGCT TTGAGTGTGT AAAGACACGC 
TATGACACCC GTCAGCGCAA ATGTCCCAAG TGTAATGCTG CGTTTGGTGC CAATGATTTC 
CACCGCATCT ACATTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281369.1
CDS	169..3009
Translation	MGEESVGGLKRQEKEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSCKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDLEEVTTQNEKLKVELRSAVEEAVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKQDPEDVPAQSSASKASQEEVNEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDSAKDKEKGKHDDGRKKEAEVIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	XM_022425661.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger protein 20 (RNF20), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022425661.1

ORF Insert Sequence:

ATGGGGGAGG AAAGTGTTGG AGGTTTGAAG AGACAGGAGA AGGAGGAGCT AGACATTCGA 
ACACTGCAGA CCAAAAATCG CAAGCTGGCA GAAATGCTGG ATCAACGGCA GGCCATCGAA 
GATGAACTCC GTGAGCACAT TGAAAAACTG GAGCGAAGAC AGGCCACTGA TGATGCCTCA 
CTGCTGATCG TCAACCGGTA CTGGAGTCAG TTTGATGAAA ACATCCGTAT CATCCTTAAA 
CGGTACGATC TGGAGCAGGG TTTGGGAGAT CTCCTCACAG AAAGGAAAGC CCTTGTCGTG 
CCCGAACCAG AGCCGGACTC TGATAGCAAT CAGGAACGGA AAGATGACCG AGAGAGAGGG 
GAAGGGCAAG AGCCAGCTTT CTCTTTCCTT GCTACTTTGG CCAGCAGTTC CAGTGAAGAA 
ATGGAATCTC AGCTGCAGGA GCGAGTGGAG TCCTCCCGCC GAGCTGTGTC CCAGATCGTG 
ACTGTCTATG ATAAATTACA AGAGAAAGTG GAGCTGTTAT CATGCAAACT AAATAGTGGA 
GATAATCTGA TAGTGGAAGA GGCGGTGCAG GAGCTGAATT CCTTCCTTGC ACAGGAGAAT 
ATGAGGCTAC AGGAATTGAC AGACCTCCTT CAGGAGAAGC ATCGCACCAT GTCTCAGGAG 
TTCTCCAAGT TACAGAGTAA AGTGGAGACA GCCGAGTCGC GAGTGTCTGT CCTAGAGTCC 
ATGATCGATG ACCTGCAGTG GGATATTGAC AAAATTCGAA AGAGGGAACA ACGACTCAAC 
CGACACTTAG CAGAAGTTCT TGAACGGGTG AATTCGAAAG GTTATAAGGT CTATGGAGCC 
GGGAGCAGTC TCTATGGTGG CACAATCACT ATCAATGCCC GGAAGTTTGA GGAAATGAAT 
GCAGAGCTGG AGGAGAACAA AGAGTTGGCT CAGAACCGTC ACTGTGAGCT GGAGAAACTT 
CGGCAAGACT TGGAGGAGGT CACTACGCAA AATGAAAAGC TGAAGGTGGA ATTGCGGAGC 
GCGGTGGAGG AGGCAGTTAA GGAGACTCCG GAATACCGCT GTATGCAGTC ACAGTTCTCC 
GTCCTGTACA ATGAGAGCCT GCAGTTGAAA GCGCACTTGG ATGAGGCTCG GACTCTGCTT 
CATGGCACCA GGGGGACCCA CCAGCGCCAA GTTGAGCTTA TTGAGCGAGA TGAAGTTAGT 
CTTCATAAGA AGCTGAGGAC TGAAGTGATC CAGCTGGAAG ATACTCTGGC CCAGGTCCGC 
AAGGAGTATG AGATGCTGAG GATAGAATTT GAGCAGACCC TTGCTGCCAA TGAGCAAGCA 
GGCCCCATAA ACCGGGAGAT GCGTCACCTC ATCAGTAGCC TCCAGAATCA CAATCACCAG 
CTGAAAGGGG AGGTCCTAAG ATATAAGCGG AAACTGAGAG AAGCCCAGTC TGACCTGAAC 
AAGACACGTC TGCGCAGTGG CAGTGCCCTC CTGCAGTCTC AGTCCAGTAC TGAGGATCCA 
AAGGATGAGC CTGCAGAGCT GAAGCAAGAT CCTGAGGACG TACCTGCCCA GTCCTCCGCA 
TCAAAGGCAT CTCAGGAGGA GGTCAATGAA ATTAAGTCCA AACGGGACGA GGAAGAACGA 
GAACGAGAAA GGAGGGAGAA AGAGAGGGAG CGAGAAAGAG AGCGGGAGAA GGAAAAGGAG 
AGGGAACGAG AGAAGCAGAA ACTGAAAGAG TCAGAAAAAG AAAGAGACTC TGCTAAGGAT 
AAAGAGAAAG GGAAACATGA CGATGGAAGG AAAAAGGAAG CAGAAGTTAT CAAACAATTG 
AAGATTGAAC TCAAGAAGGC ACAAGAGAGC CAAAAGGAGA TGAAACTATT GCTAGATATG 
TACCGCTCTG CCCCAAAGGA ACAGAGAGAC AAAGTTCAGC TGATGGCAGC TGAGAAGAAG 
TCAAAGGCAG AGTTGGAAGA TTTAAGGCAA AGACTCAAGG ATCTAGAGGA TAAGGAGAAA 
AAGGAGAACA AGAAAATGGC TGATGAGGAT GCCTTGAGGA AGATCCGGGC AGTAGAAGAG 
CAGATAGAGT ACCTGCAGAA GAAGCTGGCC ATGGCCAAGC AGGAGGAAGA GGCACTCCTC 
TCAGAGATGG ATGTTACAGG CCAGGCCTTT GAAGACATGC AGGAGCAAAA TATCCGTTTA 
ATGCAGCAGT TGCGGGAGAA GGATGATGCA AATTTCAAGC TCATGTCTGA GCGTATCAAG 
TCCAATCAGA TCCACAAGTT GCTCAAAGAA GAGAAGGAGG AGCTGGCTGA CCAAGTTTTG 
ACTCTAAAGA CTCAGGTTGA TGCCCAGTTA CAGGTAGTAA GGAAATTGGA AGAGAAGGAG 
CATCTGTTAC AAAGCAACAT TGGCACAGGG GAGAAGGAAC TGGGTCTTAG GACACAAGCC 
TTAGAGATGA ATAAACGCAA GGCGATGGAA GCAGCCCAGC TGGCAGATGA CCTCAAAGCG 
CAACTGGAAT TGGCTCAGAA GAAGCTACAT GATTTTCAGG ATGAGATCGT GGAGAATAGT 
GTCACCAAAG AAAAGGATAT GTTTAATTTC AAACGAGCTC AGGAGGACAT CTCTAGACTC 
CGAAGGAAGC TGGAGACCAC AAAGAAACCA GACAATGTGC CCAAATGTGA TGAGATCCTG 
ATGGAGGAGA TTAAGGATTA CAAGGCACGC CTGACCTGTC CATGTTGCAA TATGCGTAAA 
AAGGATGCTG TACTTACCAA GTGTTTTCAT GTTTTCTGCT TTGAGTGTGT AAAGACACGC 
TATGACACCC GTCAGCGCAA ATGTCCCAAG TGTAATGCTG CGTTTGGTGC CAATGATTTC 
CACCGCATCT ACATTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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RNF20 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

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FAX

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