COL4A6 cDNA ORF clone, Canis lupus familiaris(dog)

The following COL4A6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe285422 XM_022416201.1
Latest version!
Canis lupus familiaris collagen type IV alpha 6 chain (COL4A6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe32750 XM_845861.4
Latest version!
Canis lupus familiaris collagen, type IV, alpha 6 (COL4A6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe32750 XM_014111911.2
Latest version!
Canis lupus familiaris collagen type IV alpha 6 chain (COL4A6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe32751 XM_014111912.2
Latest version!
Canis lupus familiaris collagen type IV alpha 6 chain (COL4A6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
OCe32750 XM_014111911.1 Canis lupus familiaris collagen, type IV, alpha 6 (COL4A6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe32751 XM_014111912.1 Canis lupus familiaris collagen, type IV, alpha 6 (COL4A6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe285422
    Clone ID Related Accession (Same CDS sequence) XM_022416201.1
    Accession Version XM_022416201.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-6(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACTGAGGA ACTGGCAGGA 
    GCCGGGCAAC CAGGGCCAAT CGGAATTCAA GGTCCAACGG GTCCTCAAGG ATTTGCCGGC
    CCTAGTGGTT TAGCAGGGTT GAAAGGAGAA AGGGGCTCCC CAGGCCCCCT GGGACCATAT
    GGACCAAAAG GAGATAAGGG CCCCATGGGA GTGCCTGGCT TTCTCGGCAT CAATGGGATT
    CCGGGCCACC CTGGACAGCC AGGGCCCAGA GGCCCACCTG GCCTGGATGG TTGTAATGGA
    ACTCAAGGAG CTGTTGGATA TCCAGGCCCT AATGGCTATC CTGGGCTTCT CGGACCACCT
    GGGCTTCCTG GTCAGAAAGG CTCTAAAGGT GAACCTGTCC TTGCCCAAGG TACTTTCAAA
    GGAATGAAGG GGGAGCCTGG GCTTCCTGGA CTGGATGGAA TAATTGGCCC TCCAGGAGCA
    CCTGGGTCCC CAGGAGCCGT AGGACCTATG GGACCACCAG GATTGCAAGG TCCTCCAGGC
    CCTCCTGGCT TCCCTGGTCC TGATGGGAAT ATGGGGTTAG GTTTCCAAGG AGAGAAAGGA
    GTCAAGGGGG ATGTTGGCCT CCCAGGCCCT GCAGGACCCC CACCATCCAC TGGGGAACTG
    GAATTTATGG GATTTCCCAA AGGGAAGAAA GGATCCAAGG GTGAACCAGG GCTTCCGGGT
    TTCCCAGGAA TAAGTGGCCC TCCAGGTCTC CCGGGACTTG GAACTGCTGG AGAAAAGGGA
    GAAAAGGGAA TCCCTGGTTT GCCAGGACCA AGGGGTCCCA TGGGTTTAGA AAGACTCCAA
    GGCCCTCCAG GGATTCAGGG CAAGAAGGGG TCCTCAGGTT TTCCTGGGCT TAATGGACTC
    CCAGGAATTA AGGGTGAAAA GGGTGGCATT GGCCTGCCAG GCCCAGATGT GTTCATCGAT
    ACAGATGGTG CTGTGATCTC AGGTTATCCT GGAGATCCCG GTATGCCAGG TCTCCCAGGA
    CTTAAAGGAG ATGAAGGCAT CCAGGGCCTG CCTGGCCCTT CTGGCATCCC TGGCTTACCA
    GCTTTACCAG GTGTCCCAGG TGCTCTAGGC CCCCAGGGAG TTCCAGGTTT AAAGGGGGAC
    CAAGGAAGCC CAGGCCGTAC CACAGTTGGG ACAGCTGGCA CCCCAGGCAG AGATGGTTTG
    CCAGGCCTAC CAGGCCTACG AGGCCCACCC GGTCCAGCAT TTGAGACTGA AACCCTACAG
    AACACAGAGC CAGGGTTCCC TGGTCTCCGA GGAGAAAGAG GTCCAAAAGG AAACCCAGGC
    CTCAAAGGCA TAAAAGGAGA TTTGGGCTTT TGTGCTTGTG ACGGTGGGGT CCCTAACACT
    GGACCACCTG GGGAGCCAGG CATGCCTGGG CCACCAGGTC TCATAGGTCT TCCGGGCCTT
    AAAGGAACCA GAGGAGATCC AGGCTCTGGG GGTGCACAGG GCCCATCAGG GGCTCCAGGC
    TTATTTGGGC CTCCAGGTCC TGCGGGCTCC AAAGGAGATA AAGGGGAGCC AACTTTCAGT
    TCCGCATCAG GGGTGCCAGG GGAGCAGGGT GATCCTGGCC CCCAGGGGCT CCCTGGGGAG
    ATGGGAGCTC CAGGCAAGGA TGGAATACCA GGTCTACCAG GTCTGCCAGG TCTTCCGGGT
    GACGGTGGAC AGGGCTTCCC AGGTGAAAAG GGGTTACCAG GACTGCCTGG TGAAAGGGGC
    CACAGTGGCC CAACTGGCCC CCCAGGAATT GGGCTGCCAG GATCTCCTGG ACCTCGTGGG
    CTTCCTGGAG ATCAAGGAAT AGATGGATTA CCAGGGCAAC AAGGCCTCCC TGGGCTTCCA
    GGAATCACCT TGCCGTGTAT AATTCCTGAG ACATATGGTC TGTCTGGCTT ACCAGGAGCT
    CCCGGATTCC CAGGCCCTAA AGGGGCCCGT GGCCTCCCTG GGACCCCCGG TCAGCCTGGA
    TTGCGTGGGA ATAAAGGAGA GCCCGGAAGT CCAGGATTAG TTCACCTTCC TGAATTGCCA
    GGATTTCCTG GACCTCGTGG GGAGAAGGGC TTGCCTGGGT TTCCTGGACT CCCTGGAAAA
    GATGGCTTGC CTGGGAAACT TGGCAGTCCG GGTTTACCTG GTCCCAAGGG AGCCCCTGGT
    GACATCTTTG GTGCTGAGAA TGGTTCTCCG GGGGAGCAAG GCCTACAGGG ATTACCAGGG
    GACAGAGGAC TTCCTGGAGA CTCTGGCCTT CCAGGACCCA AGGGTTTGCT TGGAAAGTCG
    GGCCTGCTAG GCCCCAAAGG TGAACGGGGC AGCCCTGGAA CACCGGGCCA CATGGGAGAG
    CCAGGGCCCC CAGGGCCTGA TGGTCTATAC GGCAGCATCA AGGGCAAGCC AGGGCTCCCT
    GGAGTGCCAG GCTTTCCAGG CCCTTCAGGA CATCCTGGAA AGAAAGGTTT AAGAGGCGAG
    ACAGGTTCCC CTGCATCAGC TGGAAAGAGA GGCCTGCCAG GGCTGAAAGG CCTTCCGGGA
    TCTCCAGGGC TGATTGGCTT CTTGGGGAGC TCAGGCTTGC CAGGGAACAC TGGGTTGCCA
    GGCCTGCCAG GTCCAAAGGG TGAAAAGGGG TCTGTTGGGC TGGTAGGTTT TCCAGGGATG
    CCAGGTCTCC CTGGTATTCC TGGCACGAGT GGAATAAAGG GAATTTCTGG GTCAGCCGGA
    AGAGTGGGAC CATCTGGAAA GGCTGGCCAT GCTGGTGAAA AAGGAGACAG AGGCGATCCA
    GGGCCAGCTG GAGTACCCAG TCCAAGACCT CCCATGCTGA ACCTCCAGTT CAAAGGAGAC
    AAAGGCTCTA GAGGCTCAGC TGGATTGGAT GGATTTCCCG GGCCCAGAGG TGACAAAGGA
    GAGGCCGGTC CCCTGGGGCC ACCAGGCTTG CCTGGAGCTC CTGGCTTCCC CAGTACCATC
    AAGGGGCTTA TTGGGAGGGC AGGCCTCCCT GGCTCCATAG GACTCCGGGG CTTACCTGGC
    CTGAAGGGGT CCCCTGGAAT CACAGGCTTC CCGGGAATAG CAGGAGAAAG CGGTTCACAG
    GGTCTCAATG GAGCTCCTGG ACTCCCAGGA GCATCTGGTC TCCCAGGTTC AAAAGGAGAT
    CAAGGTCAAA CACTTGGAAT TTCTGGTAGC CCAGGACTCA AGGGGCAACC TGGGGAGTCT
    GGTTTTAAAG GCGTGCAAGG AACCAATGGA CTGGTTGGTG ATGTGGGTCT CCCAGGAAGC
    AAAGGGGAAG ATGGGAAAGT TGGTATTTCT GGAGACCTCG GCCTTCCTGG CTCCCCAGGA
    CTCCCCGGGA TTGCAGGCAT GAGAGGAAAT CCAGGGCTTC CGGGTTCTCC AGGCCATCCA
    GGGGCAACTG GGACCCTTGG ACCCCCTGGC CTAATAGGAA CCAAAGGTTT CCCTGGACTT
    CCTGGTTTAC ATGGACTCAA TGGGCTTCCA GGAACCAAGG GAACACATGG AACTCCAGGA
    CCTAGTATAA GTGGTGTGCC GGGGCCAGCG GGCCTGCCTG GCCCCAAAGG AGAGAAAGGA
    TCTCCAGGAA CTGGTATCGG AGCCCCAGGG AGGCCAGGCT CAAGAGGGCA AAAAGGTGGC
    CGAGGTTTCC CGGGTCTCCA GGGCCCTGCT GGTCTGCCTG GTGCCCCAGG CCTCTCCTTG
    CCTTCGATCA TAGTAGGACA GCCTGGCGAC CCTGGGCGAC CAGGGCTAGA TGGAGAGCAA
    GGCCGCCCGG GCCCCCCGGG GCCCCCGGGG CCCCCTGGGC CATCCTCCAA TCAAGGTGAC
    CCTGGAGACC CTGGCTTCCC TGGAGTTCCT GGCCCTCAAG GGCCCAAAGG AGTCCAAGGA
    ATTCCAGGTT TCTCCGGCCT CCCTGGAGAG CTAGGGCTAA AAGGCATGAG AGGTGAGCCT
    GGCTTCATGG GGATTCCAGG CAAGGTTGGG CCACCTGGAG ACCCAGGACT TCCCGGGATG
    AAGGGGAAGG CAGGGCCCCA AGGCTTTTCC GGACCCCAAG GTGCTCCTGG ACAGACAACA
    ATTGCAGAAG CTGTCCAGGT TCCTCCTGGA CCCATGGGTC TACCGGGCAT CGATGGCATC
    CCTGGCCTCA CGGGAGATCC TGGGGTTCAA GGTCCTGTAG GCCTCCAAGG CTCCAAAGGT
    TTACCTGGCG TCCCTGGCAA AGATGGTCTC AATGGAATCC CCGGTCCGCC TGGGGCTCTT
    GGCGACCCTG GTCTCCCTGG ACTGCAAGGC CCTCCAGGAT TTGAAGGAGC TCCAGGGAAG
    AAGGGTCCCT TCGGGAGGGC TGGAGTGCCT GGGCAGAGTG TGAGAGTGGG CTACACCTTG
    GTGAAGCACA GCCAGTCAGA ACAGCTGCCC CTGTGCCCTG CCGGGATGAG CCAGCTGTGG
    GTGGGTTACA GCTTGCTGTT CGTGGAGGGC CAGGAGAAAG CCCACAACCA GGACCTGGGT
    TTTGCTGGCT CCTGCCTGCC CCGCTTTAGC ACCATGCCCT TCATCTACTG CAACATCAAC
    GAAGTGTGCC ACTACGCCAG ACGCAATGAT AAATCCTACT GGCTCTCCAC CACCGCCCCC
    ATTCCCATGA TGCCCGTCGG CCAGGCCCAG ATCCCCCAGT ACATCAGCCG TTGTTCGGTG
    TGCGAGGCAC CCTCACAGGC CATTGCCGTG CACAGCCAGG ACATCACCAT CCCGCAGTGC
    CCCCTGGGCT GGCACAGCCT CTGGATTGGG TACTCCTTCC TCATGCACAC TGCCGCTGGT
    GCCGAGGGCG GCGGCCAGTC CCTGGTCTCA CCGGGCTCCT GCCTGGAGGA TTTCCGTGCT
    ACTCCTTTCA TTGAGTGCAG TGGGGCCCGG GGCACCTGCC ACTACTTCGC CAACAAGTAC
    AGTTTCTGGC TGACAACCGT GGAAGAGAGG CAGCAGTTCC GGGAGGAACC AGTCTCTGAA
    ACGCTCAAGG CTGGGCAGCT CCACACCCGA GTCAGCCGCT GCCAGGTGTG TATGAGAAGC
    CTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271909.1
    CDS505..5490
    Translation

    Target ORF information:

    RefSeq Version XM_022416201.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type IV alpha 6 chain (COL4A6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416201.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACTGAGGA ACTGGCAGGA 
    GCCGGGCAAC CAGGGCCAAT CGGAATTCAA GGTCCAACGG GTCCTCAAGG ATTTGCCGGC
    CCTAGTGGTT TAGCAGGGTT GAAAGGAGAA AGGGGCTCCC CAGGCCCCCT GGGACCATAT
    GGACCAAAAG GAGATAAGGG CCCCATGGGA GTGCCTGGCT TTCTCGGCAT CAATGGGATT
    CCGGGCCACC CTGGACAGCC AGGGCCCAGA GGCCCACCTG GCCTGGATGG TTGTAATGGA
    ACTCAAGGAG CTGTTGGATA TCCAGGCCCT AATGGCTATC CTGGGCTTCT CGGACCACCT
    GGGCTTCCTG GTCAGAAAGG CTCTAAAGGT GAACCTGTCC TTGCCCAAGG TACTTTCAAA
    GGAATGAAGG GGGAGCCTGG GCTTCCTGGA CTGGATGGAA TAATTGGCCC TCCAGGAGCA
    CCTGGGTCCC CAGGAGCCGT AGGACCTATG GGACCACCAG GATTGCAAGG TCCTCCAGGC
    CCTCCTGGCT TCCCTGGTCC TGATGGGAAT ATGGGGTTAG GTTTCCAAGG AGAGAAAGGA
    GTCAAGGGGG ATGTTGGCCT CCCAGGCCCT GCAGGACCCC CACCATCCAC TGGGGAACTG
    GAATTTATGG GATTTCCCAA AGGGAAGAAA GGATCCAAGG GTGAACCAGG GCTTCCGGGT
    TTCCCAGGAA TAAGTGGCCC TCCAGGTCTC CCGGGACTTG GAACTGCTGG AGAAAAGGGA
    GAAAAGGGAA TCCCTGGTTT GCCAGGACCA AGGGGTCCCA TGGGTTTAGA AAGACTCCAA
    GGCCCTCCAG GGATTCAGGG CAAGAAGGGG TCCTCAGGTT TTCCTGGGCT TAATGGACTC
    CCAGGAATTA AGGGTGAAAA GGGTGGCATT GGCCTGCCAG GCCCAGATGT GTTCATCGAT
    ACAGATGGTG CTGTGATCTC AGGTTATCCT GGAGATCCCG GTATGCCAGG TCTCCCAGGA
    CTTAAAGGAG ATGAAGGCAT CCAGGGCCTG CCTGGCCCTT CTGGCATCCC TGGCTTACCA
    GCTTTACCAG GTGTCCCAGG TGCTCTAGGC CCCCAGGGAG TTCCAGGTTT AAAGGGGGAC
    CAAGGAAGCC CAGGCCGTAC CACAGTTGGG ACAGCTGGCA CCCCAGGCAG AGATGGTTTG
    CCAGGCCTAC CAGGCCTACG AGGCCCACCC GGTCCAGCAT TTGAGACTGA AACCCTACAG
    AACACAGAGC CAGGGTTCCC TGGTCTCCGA GGAGAAAGAG GTCCAAAAGG AAACCCAGGC
    CTCAAAGGCA TAAAAGGAGA TTTGGGCTTT TGTGCTTGTG ACGGTGGGGT CCCTAACACT
    GGACCACCTG GGGAGCCAGG CATGCCTGGG CCACCAGGTC TCATAGGTCT TCCGGGCCTT
    AAAGGAACCA GAGGAGATCC AGGCTCTGGG GGTGCACAGG GCCCATCAGG GGCTCCAGGC
    TTATTTGGGC CTCCAGGTCC TGCGGGCTCC AAAGGAGATA AAGGGGAGCC AACTTTCAGT
    TCCGCATCAG GGGTGCCAGG GGAGCAGGGT GATCCTGGCC CCCAGGGGCT CCCTGGGGAG
    ATGGGAGCTC CAGGCAAGGA TGGAATACCA GGTCTACCAG GTCTGCCAGG TCTTCCGGGT
    GACGGTGGAC AGGGCTTCCC AGGTGAAAAG GGGTTACCAG GACTGCCTGG TGAAAGGGGC
    CACAGTGGCC CAACTGGCCC CCCAGGAATT GGGCTGCCAG GATCTCCTGG ACCTCGTGGG
    CTTCCTGGAG ATCAAGGAAT AGATGGATTA CCAGGGCAAC AAGGCCTCCC TGGGCTTCCA
    GGAATCACCT TGCCGTGTAT AATTCCTGAG ACATATGGTC TGTCTGGCTT ACCAGGAGCT
    CCCGGATTCC CAGGCCCTAA AGGGGCCCGT GGCCTCCCTG GGACCCCCGG TCAGCCTGGA
    TTGCGTGGGA ATAAAGGAGA GCCCGGAAGT CCAGGATTAG TTCACCTTCC TGAATTGCCA
    GGATTTCCTG GACCTCGTGG GGAGAAGGGC TTGCCTGGGT TTCCTGGACT CCCTGGAAAA
    GATGGCTTGC CTGGGAAACT TGGCAGTCCG GGTTTACCTG GTCCCAAGGG AGCCCCTGGT
    GACATCTTTG GTGCTGAGAA TGGTTCTCCG GGGGAGCAAG GCCTACAGGG ATTACCAGGG
    GACAGAGGAC TTCCTGGAGA CTCTGGCCTT CCAGGACCCA AGGGTTTGCT TGGAAAGTCG
    GGCCTGCTAG GCCCCAAAGG TGAACGGGGC AGCCCTGGAA CACCGGGCCA CATGGGAGAG
    CCAGGGCCCC CAGGGCCTGA TGGTCTATAC GGCAGCATCA AGGGCAAGCC AGGGCTCCCT
    GGAGTGCCAG GCTTTCCAGG CCCTTCAGGA CATCCTGGAA AGAAAGGTTT AAGAGGCGAG
    ACAGGTTCCC CTGCATCAGC TGGAAAGAGA GGCCTGCCAG GGCTGAAAGG CCTTCCGGGA
    TCTCCAGGGC TGATTGGCTT CTTGGGGAGC TCAGGCTTGC CAGGGAACAC TGGGTTGCCA
    GGCCTGCCAG GTCCAAAGGG TGAAAAGGGG TCTGTTGGGC TGGTAGGTTT TCCAGGGATG
    CCAGGTCTCC CTGGTATTCC TGGCACGAGT GGAATAAAGG GAATTTCTGG GTCAGCCGGA
    AGAGTGGGAC CATCTGGAAA GGCTGGCCAT GCTGGTGAAA AAGGAGACAG AGGCGATCCA
    GGGCCAGCTG GAGTACCCAG TCCAAGACCT CCCATGCTGA ACCTCCAGTT CAAAGGAGAC
    AAAGGCTCTA GAGGCTCAGC TGGATTGGAT GGATTTCCCG GGCCCAGAGG TGACAAAGGA
    GAGGCCGGTC CCCTGGGGCC ACCAGGCTTG CCTGGAGCTC CTGGCTTCCC CAGTACCATC
    AAGGGGCTTA TTGGGAGGGC AGGCCTCCCT GGCTCCATAG GACTCCGGGG CTTACCTGGC
    CTGAAGGGGT CCCCTGGAAT CACAGGCTTC CCGGGAATAG CAGGAGAAAG CGGTTCACAG
    GGTCTCAATG GAGCTCCTGG ACTCCCAGGA GCATCTGGTC TCCCAGGTTC AAAAGGAGAT
    CAAGGTCAAA CACTTGGAAT TTCTGGTAGC CCAGGACTCA AGGGGCAACC TGGGGAGTCT
    GGTTTTAAAG GCGTGCAAGG AACCAATGGA CTGGTTGGTG ATGTGGGTCT CCCAGGAAGC
    AAAGGGGAAG ATGGGAAAGT TGGTATTTCT GGAGACCTCG GCCTTCCTGG CTCCCCAGGA
    CTCCCCGGGA TTGCAGGCAT GAGAGGAAAT CCAGGGCTTC CGGGTTCTCC AGGCCATCCA
    GGGGCAACTG GGACCCTTGG ACCCCCTGGC CTAATAGGAA CCAAAGGTTT CCCTGGACTT
    CCTGGTTTAC ATGGACTCAA TGGGCTTCCA GGAACCAAGG GAACACATGG AACTCCAGGA
    CCTAGTATAA GTGGTGTGCC GGGGCCAGCG GGCCTGCCTG GCCCCAAAGG AGAGAAAGGA
    TCTCCAGGAA CTGGTATCGG AGCCCCAGGG AGGCCAGGCT CAAGAGGGCA AAAAGGTGGC
    CGAGGTTTCC CGGGTCTCCA GGGCCCTGCT GGTCTGCCTG GTGCCCCAGG CCTCTCCTTG
    CCTTCGATCA TAGTAGGACA GCCTGGCGAC CCTGGGCGAC CAGGGCTAGA TGGAGAGCAA
    GGCCGCCCGG GCCCCCCGGG GCCCCCGGGG CCCCCTGGGC CATCCTCCAA TCAAGGTGAC
    CCTGGAGACC CTGGCTTCCC TGGAGTTCCT GGCCCTCAAG GGCCCAAAGG AGTCCAAGGA
    ATTCCAGGTT TCTCCGGCCT CCCTGGAGAG CTAGGGCTAA AAGGCATGAG AGGTGAGCCT
    GGCTTCATGG GGATTCCAGG CAAGGTTGGG CCACCTGGAG ACCCAGGACT TCCCGGGATG
    AAGGGGAAGG CAGGGCCCCA AGGCTTTTCC GGACCCCAAG GTGCTCCTGG ACAGACAACA
    ATTGCAGAAG CTGTCCAGGT TCCTCCTGGA CCCATGGGTC TACCGGGCAT CGATGGCATC
    CCTGGCCTCA CGGGAGATCC TGGGGTTCAA GGTCCTGTAG GCCTCCAAGG CTCCAAAGGT
    TTACCTGGCG TCCCTGGCAA AGATGGTCTC AATGGAATCC CCGGTCCGCC TGGGGCTCTT
    GGCGACCCTG GTCTCCCTGG ACTGCAAGGC CCTCCAGGAT TTGAAGGAGC TCCAGGGAAG
    AAGGGTCCCT TCGGGAGGGC TGGAGTGCCT GGGCAGAGTG TGAGAGTGGG CTACACCTTG
    GTGAAGCACA GCCAGTCAGA ACAGCTGCCC CTGTGCCCTG CCGGGATGAG CCAGCTGTGG
    GTGGGTTACA GCTTGCTGTT CGTGGAGGGC CAGGAGAAAG CCCACAACCA GGACCTGGGT
    TTTGCTGGCT CCTGCCTGCC CCGCTTTAGC ACCATGCCCT TCATCTACTG CAACATCAAC
    GAAGTGTGCC ACTACGCCAG ACGCAATGAT AAATCCTACT GGCTCTCCAC CACCGCCCCC
    ATTCCCATGA TGCCCGTCGG CCAGGCCCAG ATCCCCCAGT ACATCAGCCG TTGTTCGGTG
    TGCGAGGCAC CCTCACAGGC CATTGCCGTG CACAGCCAGG ACATCACCAT CCCGCAGTGC
    CCCCTGGGCT GGCACAGCCT CTGGATTGGG TACTCCTTCC TCATGCACAC TGCCGCTGGT
    GCCGAGGGCG GCGGCCAGTC CCTGGTCTCA CCGGGCTCCT GCCTGGAGGA TTTCCGTGCT
    ACTCCTTTCA TTGAGTGCAG TGGGGCCCGG GGCACCTGCC ACTACTTCGC CAACAAGTAC
    AGTTTCTGGC TGACAACCGT GGAAGAGAGG CAGCAGTTCC GGGAGGAACC AGTCTCTGAA
    ACGCTCAAGG CTGGGCAGCT CCACACCCGA GTCAGCCGCT GCCAGGTGTG TATGAGAAGC
    CTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32750
    Clone ID Related Accession (Same CDS sequence) XM_014111911.1 , XM_845861.4 , XM_014111911.2
    Accession Version XM_014111911.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product collagen alpha-6(IV) chain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACTGAGGA ACTGGCAGGA 
    GCCGGAGAGA AGAAGTCCTA TGGAAAGCCA TGTGGTGGCC AAGACTGCAG GGGAGGCTGT
    AAATGCTTTC CTGAGAAAGG AGCGAGAGGG CAACCAGGGC CAATCGGAAT TCAAGGTCCA
    ACGGGTCCTC AAGGATTTGC CGGCCCTAGT GGTTTAGCAG GGTTGAAAGG AGAAAGGGGC
    TCCCCAGGCC CCCTGGGACC ATATGGACCA AAAGGAGATA AGGGCCCCAT GGGAGTGCCT
    GGCTTTCTCG GCATCAATGG GATTCCGGGC CACCCTGGAC AGCCAGGGCC CAGAGGCCCA
    CCTGGCCTGG ATGGTTGTAA TGGAACTCAA GGAGCTGTTG GATATCCAGG CCCTAATGGC
    TATCCTGGGC TTCTCGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTACTTT CAAAGGAATG AAGGGGGAGC CTGGGCTTCC TGGACTGGAT
    GGAATAATTG GCCCTCCAGG AGCACCTGGG TCCCCAGGAG CCGTAGGACC TATGGGACCA
    CCAGGATTGC AAGGTCCTCC AGGCCCTCCT GGCTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCAGG CCCTGCAGGA
    CCCCCACCAT CCACTGGGGA ACTGGAATTT ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCTTCC GGGTTTCCCA GGAATAAGTG GCCCTCCAGG TCTCCCGGGA
    CTTGGAACTG CTGGAGAAAA GGGAGAAAAG GGAATCCCTG GTTTGCCAGG ACCAAGGGGT
    CCCATGGGTT TAGAAAGACT CCAAGGCCCT CCAGGGATTC AGGGCAAGAA GGGGTCCTCA
    GGTTTTCCTG GGCTTAATGG ACTCCCAGGA ATTAAGGGTG AAAAGGGTGG CATTGGCCTG
    CCAGGCCCAG ATGTGTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAT
    CCCGGTATGC CAGGTCTCCC AGGACTTAAA GGAGATGAAG GCATCCAGGG CCTGCCTGGC
    CCTTCTGGCA TCCCTGGCTT ACCAGCTTTA CCAGGTGTCC CAGGTGCTCT AGGCCCCCAG
    GGAGTTCCAG GTTTAAAGGG GGACCAAGGA AGCCCAGGCC GTACCACAGT TGGGACAGCT
    GGCACCCCAG GCAGAGATGG TTTGCCAGGC CTACCAGGCC TACGAGGCCC ACCCGGTCCA
    GCATTTGAGA CTGAAACCCT ACAGAACACA GAGCCAGGGT TCCCTGGTCT CCGAGGAGAA
    AGAGGTCCAA AAGGAAACCC AGGCCTCAAA GGCATAAAAG GAGATTTGGG CTTTTGTGCT
    TGTGACGGTG GGGTCCCTAA CACTGGACCA CCTGGGGAGC CAGGCATGCC TGGGCCACCA
    GGTCTCATAG GTCTTCCGGG CCTTAAAGGA ACCAGAGGAG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGCTCC AGGCTTATTT GGGCCTCCAG GTCCTGCGGG CTCCAAAGGA
    GATAAAGGGG AGCCAACTTT CAGTTCCGCA TCAGGGGTGC CAGGGGAGCA GGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG GGAGATGGGA GCTCCAGGCA AGGATGGAAT ACCAGGTCTA
    CCAGGTCTGC CAGGTCTTCC GGGTGACGGT GGACAGGGCT TCCCAGGTGA AAAGGGGTTA
    CCAGGACTGC CTGGTGAAAG GGGCCACAGT GGCCCAACTG GCCCCCCAGG AATTGGGCTG
    CCAGGATCTC CTGGACCTCG TGGGCTTCCT GGAGATCAAG GAATAGATGG ATTACCAGGG
    CAACAAGGCC TCCCTGGGCT TCCAGGAATC ACCTTGCCGT GTATAATTCC TGAGACATAT
    GGTCTGTCTG GCTTACCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGACCC CCGGTCAGCC TGGATTGCGT GGGAATAAAG GAGAGCCCGG AAGTCCAGGA
    TTAGTTCACC TTCCTGAATT GCCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT
    GGGTTTCCTG GACTCCCTGG AAAAGATGGC TTGCCTGGGA AACTTGGCAG TCCGGGTTTA
    CCTGGTCCCA AGGGAGCCCC TGGTGACATC TTTGGTGCTG AGAATGGTTC TCCGGGGGAG
    CAAGGCCTAC AGGGATTACC AGGGGACAGA GGACTTCCTG GAGACTCTGG CCTTCCAGGA
    CCCAAGGGTT TGCTTGGAAA GTCGGGCCTG CTAGGCCCCA AAGGTGAACG GGGCAGCCCT
    GGAACACCGG GCCACATGGG AGAGCCAGGG CCCCCAGGGC CTGATGGTCT ATACGGCAGC
    ATCAAGGGCA AGCCAGGGCT CCCTGGAGTG CCAGGCTTTC CAGGCCCTTC AGGACATCCT
    GGAAAGAAAG GTTTAAGAGG CGAGACAGGT TCCCCTGCAT CAGCTGGAAA GAGAGGCCTG
    CCAGGGCTGA AAGGCCTTCC GGGATCTCCA GGGCTGATTG GCTTCTTGGG GAGCTCAGGC
    TTGCCAGGGA ACACTGGGTT GCCAGGCCTG CCAGGTCCAA AGGGTGAAAA GGGGTCTGTT
    GGGCTGGTAG GTTTTCCAGG GATGCCAGGT CTCCCTGGTA TTCCTGGCAC GAGTGGAATA
    AAGGGAATTT CTGGGTCAGC CGGAAGAGTG GGACCATCTG GAAAGGCTGG CCATGCTGGT
    GAAAAAGGAG ACAGAGGCGA TCCAGGGCCA GCTGGAGTAC CCAGTCCAAG ACCTCCCATG
    CTGAACCTCC AGTTCAAAGG AGACAAAGGC TCTAGAGGCT CAGCTGGATT GGATGGATTT
    CCCGGGCCCA GAGGTGACAA AGGAGAGGCC GGTCCCCTGG GGCCACCAGG CTTGCCTGGA
    GCTCCTGGCT TCCCCAGTAC CATCAAGGGG CTTATTGGGA GGGCAGGCCT CCCTGGCTCC
    ATAGGACTCC GGGGCTTACC TGGCCTGAAG GGGTCCCCTG GAATCACAGG CTTCCCGGGA
    ATAGCAGGAG AAAGCGGTTC ACAGGGTCTC AATGGAGCTC CTGGACTCCC AGGAGCATCT
    GGTCTCCCAG GTTCAAAAGG AGATCAAGGT CAAACACTTG GAATTTCTGG TAGCCCAGGA
    CTCAAGGGGC AACCTGGGGA GTCTGGTTTT AAAGGCGTGC AAGGAACCAA TGGACTGGTT
    GGTGATGTGG GTCTCCCAGG AAGCAAAGGG GAAGATGGGA AAGTTGGTAT TTCTGGAGAC
    CTCGGCCTTC CTGGCTCCCC AGGACTCCCC GGGATTGCAG GCATGAGAGG AAATCCAGGG
    CTTCCGGGTT CTCCAGGCCA TCCAGGGGCA ACTGGGACCC TTGGACCCCC TGGCCTAATA
    GGAACCAAAG GTTTCCCTGG ACTTCCTGGT TTACATGGAC TCAATGGGCT TCCAGGAACC
    AAGGGAACAC ATGGAACTCC AGGACCTAGT ATAAGTGGTG TGCCGGGGCC AGCGGGCCTG
    CCTGGCCCCA AAGGAGAGAA AGGATCTCCA GGAACTGGTA TCGGAGCCCC AGGGAGGCCA
    GGCTCAAGAG GGCAAAAAGG TGGCCGAGGT TTCCCGGGTC TCCAGGGCCC TGCTGGTCTG
    CCTGGTGCCC CAGGCCTCTC CTTGCCTTCG ATCATAGTAG GACAGCCTGG CGACCCTGGG
    CGACCAGGGC TAGATGGAGA GCAAGGCCGC CCGGGCCCCC CGGGGCCCCC GGGGCCCCCT
    GGGCCATCCT CCAATCAAGG TGACCCTGGA GACCCTGGCT TCCCTGGAGT TCCTGGCCCT
    CAAGGGCCCA AAGGAGTCCA AGGAATTCCA GGTTTCTCCG GCCTCCCTGG AGAGCTAGGG
    CTAAAAGGCA TGAGAGGTGA GCCTGGCTTC ATGGGGATTC CAGGCAAGGT TGGGCCACCT
    GGAGACCCAG GACTTCCCGG GATGAAGGGG AAGGCAGGGC CCCAAGGCTT TTCCGGACCC
    CAAGGTGCTC CTGGACAGAC AACAATTGCA GAAGCTGTCC AGGTTCCTCC TGGACCCATG
    GGTCTACCGG GCATCGATGG CATCCCTGGC CTCACGGGAG ATCCTGGGGT TCAAGGTCCT
    GTAGGCCTCC AAGGCTCCAA AGGTTTACCT GGCGTCCCTG GCAAAGATGG TCTCAATGGA
    ATCCCCGGTC CGCCTGGGGC TCTTGGCGAC CCTGGTCTCC CTGGACTGCA AGGCCCTCCA
    GGATTTGAAG GAGCTCCAGG GAAGAAGGGT CCCTTCGGGA GGGCTGGAGT GCCTGGGCAG
    AGTGTGAGAG TGGGCTACAC CTTGGTGAAG CACAGCCAGT CAGAACAGCT GCCCCTGTGC
    CCTGCCGGGA TGAGCCAGCT GTGGGTGGGT TACAGCTTGC TGTTCGTGGA GGGCCAGGAG
    AAAGCCCACA ACCAGGACCT GGGTTTTGCT GGCTCCTGCC TGCCCCGCTT TAGCACCATG
    CCCTTCATCT ACTGCAACAT CAACGAAGTG TGCCACTACG CCAGACGCAA TGATAAATCC
    TACTGGCTCT CCACCACCGC CCCCATTCCC ATGATGCCCG TCGGCCAGGC CCAGATCCCC
    CAGTACATCA GCCGTTGTTC GGTGTGCGAG GCACCCTCAC AGGCCATTGC CGTGCACAGC
    CAGGACATCA CCATCCCGCA GTGCCCCCTG GGCTGGCACA GCCTCTGGAT TGGGTACTCC
    TTCCTCATGC ACACTGCCGC TGGTGCCGAG GGCGGCGGCC AGTCCCTGGT CTCACCGGGC
    TCCTGCCTGG AGGATTTCCG TGCTACTCCT TTCATTGAGT GCAGTGGGGC CCGGGGCACC
    TGCCACTACT TCGCCAACAA GTACAGTTTC TGGCTGACAA CCGTGGAAGA GAGGCAGCAG
    TTCCGGGAGG AACCAGTCTC TGAAACGCTC AAGGCTGGGC AGCTCCACAC CCGAGTCAGC
    CGCTGCCAGG TGTGTATGAG AAGCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967386.1
    CDS254..5323
    Translation

    Target ORF information:

    RefSeq Version XM_014111911.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris collagen, type IV, alpha 6 (COL4A6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111911.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACTGAGGA ACTGGCAGGA 
    GCCGGAGAGA AGAAGTCCTA TGGAAAGCCA TGTGGTGGCC AAGACTGCAG GGGAGGCTGT
    AAATGCTTTC CTGAGAAAGG AGCGAGAGGG CAACCAGGGC CAATCGGAAT TCAAGGTCCA
    ACGGGTCCTC AAGGATTTGC CGGCCCTAGT GGTTTAGCAG GGTTGAAAGG AGAAAGGGGC
    TCCCCAGGCC CCCTGGGACC ATATGGACCA AAAGGAGATA AGGGCCCCAT GGGAGTGCCT
    GGCTTTCTCG GCATCAATGG GATTCCGGGC CACCCTGGAC AGCCAGGGCC CAGAGGCCCA
    CCTGGCCTGG ATGGTTGTAA TGGAACTCAA GGAGCTGTTG GATATCCAGG CCCTAATGGC
    TATCCTGGGC TTCTCGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTACTTT CAAAGGAATG AAGGGGGAGC CTGGGCTTCC TGGACTGGAT
    GGAATAATTG GCCCTCCAGG AGCACCTGGG TCCCCAGGAG CCGTAGGACC TATGGGACCA
    CCAGGATTGC AAGGTCCTCC AGGCCCTCCT GGCTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCAGG CCCTGCAGGA
    CCCCCACCAT CCACTGGGGA ACTGGAATTT ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCTTCC GGGTTTCCCA GGAATAAGTG GCCCTCCAGG TCTCCCGGGA
    CTTGGAACTG CTGGAGAAAA GGGAGAAAAG GGAATCCCTG GTTTGCCAGG ACCAAGGGGT
    CCCATGGGTT TAGAAAGACT CCAAGGCCCT CCAGGGATTC AGGGCAAGAA GGGGTCCTCA
    GGTTTTCCTG GGCTTAATGG ACTCCCAGGA ATTAAGGGTG AAAAGGGTGG CATTGGCCTG
    CCAGGCCCAG ATGTGTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAT
    CCCGGTATGC CAGGTCTCCC AGGACTTAAA GGAGATGAAG GCATCCAGGG CCTGCCTGGC
    CCTTCTGGCA TCCCTGGCTT ACCAGCTTTA CCAGGTGTCC CAGGTGCTCT AGGCCCCCAG
    GGAGTTCCAG GTTTAAAGGG GGACCAAGGA AGCCCAGGCC GTACCACAGT TGGGACAGCT
    GGCACCCCAG GCAGAGATGG TTTGCCAGGC CTACCAGGCC TACGAGGCCC ACCCGGTCCA
    GCATTTGAGA CTGAAACCCT ACAGAACACA GAGCCAGGGT TCCCTGGTCT CCGAGGAGAA
    AGAGGTCCAA AAGGAAACCC AGGCCTCAAA GGCATAAAAG GAGATTTGGG CTTTTGTGCT
    TGTGACGGTG GGGTCCCTAA CACTGGACCA CCTGGGGAGC CAGGCATGCC TGGGCCACCA
    GGTCTCATAG GTCTTCCGGG CCTTAAAGGA ACCAGAGGAG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGCTCC AGGCTTATTT GGGCCTCCAG GTCCTGCGGG CTCCAAAGGA
    GATAAAGGGG AGCCAACTTT CAGTTCCGCA TCAGGGGTGC CAGGGGAGCA GGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG GGAGATGGGA GCTCCAGGCA AGGATGGAAT ACCAGGTCTA
    CCAGGTCTGC CAGGTCTTCC GGGTGACGGT GGACAGGGCT TCCCAGGTGA AAAGGGGTTA
    CCAGGACTGC CTGGTGAAAG GGGCCACAGT GGCCCAACTG GCCCCCCAGG AATTGGGCTG
    CCAGGATCTC CTGGACCTCG TGGGCTTCCT GGAGATCAAG GAATAGATGG ATTACCAGGG
    CAACAAGGCC TCCCTGGGCT TCCAGGAATC ACCTTGCCGT GTATAATTCC TGAGACATAT
    GGTCTGTCTG GCTTACCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGACCC CCGGTCAGCC TGGATTGCGT GGGAATAAAG GAGAGCCCGG AAGTCCAGGA
    TTAGTTCACC TTCCTGAATT GCCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT
    GGGTTTCCTG GACTCCCTGG AAAAGATGGC TTGCCTGGGA AACTTGGCAG TCCGGGTTTA
    CCTGGTCCCA AGGGAGCCCC TGGTGACATC TTTGGTGCTG AGAATGGTTC TCCGGGGGAG
    CAAGGCCTAC AGGGATTACC AGGGGACAGA GGACTTCCTG GAGACTCTGG CCTTCCAGGA
    CCCAAGGGTT TGCTTGGAAA GTCGGGCCTG CTAGGCCCCA AAGGTGAACG GGGCAGCCCT
    GGAACACCGG GCCACATGGG AGAGCCAGGG CCCCCAGGGC CTGATGGTCT ATACGGCAGC
    ATCAAGGGCA AGCCAGGGCT CCCTGGAGTG CCAGGCTTTC CAGGCCCTTC AGGACATCCT
    GGAAAGAAAG GTTTAAGAGG CGAGACAGGT TCCCCTGCAT CAGCTGGAAA GAGAGGCCTG
    CCAGGGCTGA AAGGCCTTCC GGGATCTCCA GGGCTGATTG GCTTCTTGGG GAGCTCAGGC
    TTGCCAGGGA ACACTGGGTT GCCAGGCCTG CCAGGTCCAA AGGGTGAAAA GGGGTCTGTT
    GGGCTGGTAG GTTTTCCAGG GATGCCAGGT CTCCCTGGTA TTCCTGGCAC GAGTGGAATA
    AAGGGAATTT CTGGGTCAGC CGGAAGAGTG GGACCATCTG GAAAGGCTGG CCATGCTGGT
    GAAAAAGGAG ACAGAGGCGA TCCAGGGCCA GCTGGAGTAC CCAGTCCAAG ACCTCCCATG
    CTGAACCTCC AGTTCAAAGG AGACAAAGGC TCTAGAGGCT CAGCTGGATT GGATGGATTT
    CCCGGGCCCA GAGGTGACAA AGGAGAGGCC GGTCCCCTGG GGCCACCAGG CTTGCCTGGA
    GCTCCTGGCT TCCCCAGTAC CATCAAGGGG CTTATTGGGA GGGCAGGCCT CCCTGGCTCC
    ATAGGACTCC GGGGCTTACC TGGCCTGAAG GGGTCCCCTG GAATCACAGG CTTCCCGGGA
    ATAGCAGGAG AAAGCGGTTC ACAGGGTCTC AATGGAGCTC CTGGACTCCC AGGAGCATCT
    GGTCTCCCAG GTTCAAAAGG AGATCAAGGT CAAACACTTG GAATTTCTGG TAGCCCAGGA
    CTCAAGGGGC AACCTGGGGA GTCTGGTTTT AAAGGCGTGC AAGGAACCAA TGGACTGGTT
    GGTGATGTGG GTCTCCCAGG AAGCAAAGGG GAAGATGGGA AAGTTGGTAT TTCTGGAGAC
    CTCGGCCTTC CTGGCTCCCC AGGACTCCCC GGGATTGCAG GCATGAGAGG AAATCCAGGG
    CTTCCGGGTT CTCCAGGCCA TCCAGGGGCA ACTGGGACCC TTGGACCCCC TGGCCTAATA
    GGAACCAAAG GTTTCCCTGG ACTTCCTGGT TTACATGGAC TCAATGGGCT TCCAGGAACC
    AAGGGAACAC ATGGAACTCC AGGACCTAGT ATAAGTGGTG TGCCGGGGCC AGCGGGCCTG
    CCTGGCCCCA AAGGAGAGAA AGGATCTCCA GGAACTGGTA TCGGAGCCCC AGGGAGGCCA
    GGCTCAAGAG GGCAAAAAGG TGGCCGAGGT TTCCCGGGTC TCCAGGGCCC TGCTGGTCTG
    CCTGGTGCCC CAGGCCTCTC CTTGCCTTCG ATCATAGTAG GACAGCCTGG CGACCCTGGG
    CGACCAGGGC TAGATGGAGA GCAAGGCCGC CCGGGCCCCC CGGGGCCCCC GGGGCCCCCT
    GGGCCATCCT CCAATCAAGG TGACCCTGGA GACCCTGGCT TCCCTGGAGT TCCTGGCCCT
    CAAGGGCCCA AAGGAGTCCA AGGAATTCCA GGTTTCTCCG GCCTCCCTGG AGAGCTAGGG
    CTAAAAGGCA TGAGAGGTGA GCCTGGCTTC ATGGGGATTC CAGGCAAGGT TGGGCCACCT
    GGAGACCCAG GACTTCCCGG GATGAAGGGG AAGGCAGGGC CCCAAGGCTT TTCCGGACCC
    CAAGGTGCTC CTGGACAGAC AACAATTGCA GAAGCTGTCC AGGTTCCTCC TGGACCCATG
    GGTCTACCGG GCATCGATGG CATCCCTGGC CTCACGGGAG ATCCTGGGGT TCAAGGTCCT
    GTAGGCCTCC AAGGCTCCAA AGGTTTACCT GGCGTCCCTG GCAAAGATGG TCTCAATGGA
    ATCCCCGGTC CGCCTGGGGC TCTTGGCGAC CCTGGTCTCC CTGGACTGCA AGGCCCTCCA
    GGATTTGAAG GAGCTCCAGG GAAGAAGGGT CCCTTCGGGA GGGCTGGAGT GCCTGGGCAG
    AGTGTGAGAG TGGGCTACAC CTTGGTGAAG CACAGCCAGT CAGAACAGCT GCCCCTGTGC
    CCTGCCGGGA TGAGCCAGCT GTGGGTGGGT TACAGCTTGC TGTTCGTGGA GGGCCAGGAG
    AAAGCCCACA ACCAGGACCT GGGTTTTGCT GGCTCCTGCC TGCCCCGCTT TAGCACCATG
    CCCTTCATCT ACTGCAACAT CAACGAAGTG TGCCACTACG CCAGACGCAA TGATAAATCC
    TACTGGCTCT CCACCACCGC CCCCATTCCC ATGATGCCCG TCGGCCAGGC CCAGATCCCC
    CAGTACATCA GCCGTTGTTC GGTGTGCGAG GCACCCTCAC AGGCCATTGC CGTGCACAGC
    CAGGACATCA CCATCCCGCA GTGCCCCCTG GGCTGGCACA GCCTCTGGAT TGGGTACTCC
    TTCCTCATGC ACACTGCCGC TGGTGCCGAG GGCGGCGGCC AGTCCCTGGT CTCACCGGGC
    TCCTGCCTGG AGGATTTCCG TGCTACTCCT TTCATTGAGT GCAGTGGGGC CCGGGGCACC
    TGCCACTACT TCGCCAACAA GTACAGTTTC TGGCTGACAA CCGTGGAAGA GAGGCAGCAG
    TTCCGGGAGG AACCAGTCTC TGAAACGCTC AAGGCTGGGC AGCTCCACAC CCGAGTCAGC
    CGCTGCCAGG TGTGTATGAG AAGCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32750
    Clone ID Related Accession (Same CDS sequence) XM_014111911.1 , XM_845861.4 , XM_014111911.2
    Accession Version XM_845861.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product collagen alpha-6(IV) chain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_845861.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACTGAGGA ACTGGCAGGA 
    GCCGGAGAGA AGAAGTCCTA TGGAAAGCCA TGTGGTGGCC AAGACTGCAG GGGAGGCTGT
    AAATGCTTTC CTGAGAAAGG AGCGAGAGGG CAACCAGGGC CAATCGGAAT TCAAGGTCCA
    ACGGGTCCTC AAGGATTTGC CGGCCCTAGT GGTTTAGCAG GGTTGAAAGG AGAAAGGGGC
    TCCCCAGGCC CCCTGGGACC ATATGGACCA AAAGGAGATA AGGGCCCCAT GGGAGTGCCT
    GGCTTTCTCG GCATCAATGG GATTCCGGGC CACCCTGGAC AGCCAGGGCC CAGAGGCCCA
    CCTGGCCTGG ATGGTTGTAA TGGAACTCAA GGAGCTGTTG GATATCCAGG CCCTAATGGC
    TATCCTGGGC TTCTCGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTACTTT CAAAGGAATG AAGGGGGAGC CTGGGCTTCC TGGACTGGAT
    GGAATAATTG GCCCTCCAGG AGCACCTGGG TCCCCAGGAG CCGTAGGACC TATGGGACCA
    CCAGGATTGC AAGGTCCTCC AGGCCCTCCT GGCTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCAGG CCCTGCAGGA
    CCCCCACCAT CCACTGGGGA ACTGGAATTT ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCTTCC GGGTTTCCCA GGAATAAGTG GCCCTCCAGG TCTCCCGGGA
    CTTGGAACTG CTGGAGAAAA GGGAGAAAAG GGAATCCCTG GTTTGCCAGG ACCAAGGGGT
    CCCATGGGTT TAGAAAGACT CCAAGGCCCT CCAGGGATTC AGGGCAAGAA GGGGTCCTCA
    GGTTTTCCTG GGCTTAATGG ACTCCCAGGA ATTAAGGGTG AAAAGGGTGG CATTGGCCTG
    CCAGGCCCAG ATGTGTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAT
    CCCGGTATGC CAGGTCTCCC AGGACTTAAA GGAGATGAAG GCATCCAGGG CCTGCCTGGC
    CCTTCTGGCA TCCCTGGCTT ACCAGCTTTA CCAGGTGTCC CAGGTGCTCT AGGCCCCCAG
    GGAGTTCCAG GTTTAAAGGG GGACCAAGGA AGCCCAGGCC GTACCACAGT TGGGACAGCT
    GGCACCCCAG GCAGAGATGG TTTGCCAGGC CTACCAGGCC TACGAGGCCC ACCCGGTCCA
    GCATTTGAGA CTGAAACCCT ACAGAACACA GAGCCAGGGT TCCCTGGTCT CCGAGGAGAA
    AGAGGTCCAA AAGGAAACCC AGGCCTCAAA GGCATAAAAG GAGATTTGGG CTTTTGTGCT
    TGTGACGGTG GGGTCCCTAA CACTGGACCA CCTGGGGAGC CAGGCATGCC TGGGCCACCA
    GGTCTCATAG GTCTTCCGGG CCTTAAAGGA ACCAGAGGAG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGCTCC AGGCTTATTT GGGCCTCCAG GTCCTGCGGG CTCCAAAGGA
    GATAAAGGGG AGCCAACTTT CAGTTCCGCA TCAGGGGTGC CAGGGGAGCA GGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG GGAGATGGGA GCTCCAGGCA AGGATGGAAT ACCAGGTCTA
    CCAGGTCTGC CAGGTCTTCC GGGTGACGGT GGACAGGGCT TCCCAGGTGA AAAGGGGTTA
    CCAGGACTGC CTGGTGAAAG GGGCCACAGT GGCCCAACTG GCCCCCCAGG AATTGGGCTG
    CCAGGATCTC CTGGACCTCG TGGGCTTCCT GGAGATCAAG GAATAGATGG ATTACCAGGG
    CAACAAGGCC TCCCTGGGCT TCCAGGAATC ACCTTGCCGT GTATAATTCC TGAGACATAT
    GGTCTGTCTG GCTTACCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGACCC CCGGTCAGCC TGGATTGCGT GGGAATAAAG GAGAGCCCGG AAGTCCAGGA
    TTAGTTCACC TTCCTGAATT GCCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT
    GGGTTTCCTG GACTCCCTGG AAAAGATGGC TTGCCTGGGA AACTTGGCAG TCCGGGTTTA
    CCTGGTCCCA AGGGAGCCCC TGGTGACATC TTTGGTGCTG AGAATGGTTC TCCGGGGGAG
    CAAGGCCTAC AGGGATTACC AGGGGACAGA GGACTTCCTG GAGACTCTGG CCTTCCAGGA
    CCCAAGGGTT TGCTTGGAAA GTCGGGCCTG CTAGGCCCCA AAGGTGAACG GGGCAGCCCT
    GGAACACCGG GCCACATGGG AGAGCCAGGG CCCCCAGGGC CTGATGGTCT ATACGGCAGC
    ATCAAGGGCA AGCCAGGGCT CCCTGGAGTG CCAGGCTTTC CAGGCCCTTC AGGACATCCT
    GGAAAGAAAG GTTTAAGAGG CGAGACAGGT TCCCCTGCAT CAGCTGGAAA GAGAGGCCTG
    CCAGGGCTGA AAGGCCTTCC GGGATCTCCA GGGCTGATTG GCTTCTTGGG GAGCTCAGGC
    TTGCCAGGGA ACACTGGGTT GCCAGGCCTG CCAGGTCCAA AGGGTGAAAA GGGGTCTGTT
    GGGCTGGTAG GTTTTCCAGG GATGCCAGGT CTCCCTGGTA TTCCTGGCAC GAGTGGAATA
    AAGGGAATTT CTGGGTCAGC CGGAAGAGTG GGACCATCTG GAAAGGCTGG CCATGCTGGT
    GAAAAAGGAG ACAGAGGCGA TCCAGGGCCA GCTGGAGTAC CCAGTCCAAG ACCTCCCATG
    CTGAACCTCC AGTTCAAAGG AGACAAAGGC TCTAGAGGCT CAGCTGGATT GGATGGATTT
    CCCGGGCCCA GAGGTGACAA AGGAGAGGCC GGTCCCCTGG GGCCACCAGG CTTGCCTGGA
    GCTCCTGGCT TCCCCAGTAC CATCAAGGGG CTTATTGGGA GGGCAGGCCT CCCTGGCTCC
    ATAGGACTCC GGGGCTTACC TGGCCTGAAG GGGTCCCCTG GAATCACAGG CTTCCCGGGA
    ATAGCAGGAG AAAGCGGTTC ACAGGGTCTC AATGGAGCTC CTGGACTCCC AGGAGCATCT
    GGTCTCCCAG GTTCAAAAGG AGATCAAGGT CAAACACTTG GAATTTCTGG TAGCCCAGGA
    CTCAAGGGGC AACCTGGGGA GTCTGGTTTT AAAGGCGTGC AAGGAACCAA TGGACTGGTT
    GGTGATGTGG GTCTCCCAGG AAGCAAAGGG GAAGATGGGA AAGTTGGTAT TTCTGGAGAC
    CTCGGCCTTC CTGGCTCCCC AGGACTCCCC GGGATTGCAG GCATGAGAGG AAATCCAGGG
    CTTCCGGGTT CTCCAGGCCA TCCAGGGGCA ACTGGGACCC TTGGACCCCC TGGCCTAATA
    GGAACCAAAG GTTTCCCTGG ACTTCCTGGT TTACATGGAC TCAATGGGCT TCCAGGAACC
    AAGGGAACAC ATGGAACTCC AGGACCTAGT ATAAGTGGTG TGCCGGGGCC AGCGGGCCTG
    CCTGGCCCCA AAGGAGAGAA AGGATCTCCA GGAACTGGTA TCGGAGCCCC AGGGAGGCCA
    GGCTCAAGAG GGCAAAAAGG TGGCCGAGGT TTCCCGGGTC TCCAGGGCCC TGCTGGTCTG
    CCTGGTGCCC CAGGCCTCTC CTTGCCTTCG ATCATAGTAG GACAGCCTGG CGACCCTGGG
    CGACCAGGGC TAGATGGAGA GCAAGGCCGC CCGGGCCCCC CGGGGCCCCC GGGGCCCCCT
    GGGCCATCCT CCAATCAAGG TGACCCTGGA GACCCTGGCT TCCCTGGAGT TCCTGGCCCT
    CAAGGGCCCA AAGGAGTCCA AGGAATTCCA GGTTTCTCCG GCCTCCCTGG AGAGCTAGGG
    CTAAAAGGCA TGAGAGGTGA GCCTGGCTTC ATGGGGATTC CAGGCAAGGT TGGGCCACCT
    GGAGACCCAG GACTTCCCGG GATGAAGGGG AAGGCAGGGC CCCAAGGCTT TTCCGGACCC
    CAAGGTGCTC CTGGACAGAC AACAATTGCA GAAGCTGTCC AGGTTCCTCC TGGACCCATG
    GGTCTACCGG GCATCGATGG CATCCCTGGC CTCACGGGAG ATCCTGGGGT TCAAGGTCCT
    GTAGGCCTCC AAGGCTCCAA AGGTTTACCT GGCGTCCCTG GCAAAGATGG TCTCAATGGA
    ATCCCCGGTC CGCCTGGGGC TCTTGGCGAC CCTGGTCTCC CTGGACTGCA AGGCCCTCCA
    GGATTTGAAG GAGCTCCAGG GAAGAAGGGT CCCTTCGGGA GGGCTGGAGT GCCTGGGCAG
    AGTGTGAGAG TGGGCTACAC CTTGGTGAAG CACAGCCAGT CAGAACAGCT GCCCCTGTGC
    CCTGCCGGGA TGAGCCAGCT GTGGGTGGGT TACAGCTTGC TGTTCGTGGA GGGCCAGGAG
    AAAGCCCACA ACCAGGACCT GGGTTTTGCT GGCTCCTGCC TGCCCCGCTT TAGCACCATG
    CCCTTCATCT ACTGCAACAT CAACGAAGTG TGCCACTACG CCAGACGCAA TGATAAATCC
    TACTGGCTCT CCACCACCGC CCCCATTCCC ATGATGCCCG TCGGCCAGGC CCAGATCCCC
    CAGTACATCA GCCGTTGTTC GGTGTGCGAG GCACCCTCAC AGGCCATTGC CGTGCACAGC
    CAGGACATCA CCATCCCGCA GTGCCCCCTG GGCTGGCACA GCCTCTGGAT TGGGTACTCC
    TTCCTCATGC ACACTGCCGC TGGTGCCGAG GGCGGCGGCC AGTCCCTGGT CTCACCGGGC
    TCCTGCCTGG AGGATTTCCG TGCTACTCCT TTCATTGAGT GCAGTGGGGC CCGGGGCACC
    TGCCACTACT TCGCCAACAA GTACAGTTTC TGGCTGACAA CCGTGGAAGA GAGGCAGCAG
    TTCCGGGAGG AACCAGTCTC TGAAACGCTC AAGGCTGGGC AGCTCCACAC CCGAGTCAGC
    CGCTGCCAGG TGTGTATGAG AAGCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_850954.1
    CDS614..5683
    Translation

    Target ORF information:

    RefSeq Version XM_845861.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris collagen, type IV, alpha 6 (COL4A6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_845861.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACTGAGGA ACTGGCAGGA 
    GCCGGAGAGA AGAAGTCCTA TGGAAAGCCA TGTGGTGGCC AAGACTGCAG GGGAGGCTGT
    AAATGCTTTC CTGAGAAAGG AGCGAGAGGG CAACCAGGGC CAATCGGAAT TCAAGGTCCA
    ACGGGTCCTC AAGGATTTGC CGGCCCTAGT GGTTTAGCAG GGTTGAAAGG AGAAAGGGGC
    TCCCCAGGCC CCCTGGGACC ATATGGACCA AAAGGAGATA AGGGCCCCAT GGGAGTGCCT
    GGCTTTCTCG GCATCAATGG GATTCCGGGC CACCCTGGAC AGCCAGGGCC CAGAGGCCCA
    CCTGGCCTGG ATGGTTGTAA TGGAACTCAA GGAGCTGTTG GATATCCAGG CCCTAATGGC
    TATCCTGGGC TTCTCGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTACTTT CAAAGGAATG AAGGGGGAGC CTGGGCTTCC TGGACTGGAT
    GGAATAATTG GCCCTCCAGG AGCACCTGGG TCCCCAGGAG CCGTAGGACC TATGGGACCA
    CCAGGATTGC AAGGTCCTCC AGGCCCTCCT GGCTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCAGG CCCTGCAGGA
    CCCCCACCAT CCACTGGGGA ACTGGAATTT ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCTTCC GGGTTTCCCA GGAATAAGTG GCCCTCCAGG TCTCCCGGGA
    CTTGGAACTG CTGGAGAAAA GGGAGAAAAG GGAATCCCTG GTTTGCCAGG ACCAAGGGGT
    CCCATGGGTT TAGAAAGACT CCAAGGCCCT CCAGGGATTC AGGGCAAGAA GGGGTCCTCA
    GGTTTTCCTG GGCTTAATGG ACTCCCAGGA ATTAAGGGTG AAAAGGGTGG CATTGGCCTG
    CCAGGCCCAG ATGTGTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAT
    CCCGGTATGC CAGGTCTCCC AGGACTTAAA GGAGATGAAG GCATCCAGGG CCTGCCTGGC
    CCTTCTGGCA TCCCTGGCTT ACCAGCTTTA CCAGGTGTCC CAGGTGCTCT AGGCCCCCAG
    GGAGTTCCAG GTTTAAAGGG GGACCAAGGA AGCCCAGGCC GTACCACAGT TGGGACAGCT
    GGCACCCCAG GCAGAGATGG TTTGCCAGGC CTACCAGGCC TACGAGGCCC ACCCGGTCCA
    GCATTTGAGA CTGAAACCCT ACAGAACACA GAGCCAGGGT TCCCTGGTCT CCGAGGAGAA
    AGAGGTCCAA AAGGAAACCC AGGCCTCAAA GGCATAAAAG GAGATTTGGG CTTTTGTGCT
    TGTGACGGTG GGGTCCCTAA CACTGGACCA CCTGGGGAGC CAGGCATGCC TGGGCCACCA
    GGTCTCATAG GTCTTCCGGG CCTTAAAGGA ACCAGAGGAG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGCTCC AGGCTTATTT GGGCCTCCAG GTCCTGCGGG CTCCAAAGGA
    GATAAAGGGG AGCCAACTTT CAGTTCCGCA TCAGGGGTGC CAGGGGAGCA GGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG GGAGATGGGA GCTCCAGGCA AGGATGGAAT ACCAGGTCTA
    CCAGGTCTGC CAGGTCTTCC GGGTGACGGT GGACAGGGCT TCCCAGGTGA AAAGGGGTTA
    CCAGGACTGC CTGGTGAAAG GGGCCACAGT GGCCCAACTG GCCCCCCAGG AATTGGGCTG
    CCAGGATCTC CTGGACCTCG TGGGCTTCCT GGAGATCAAG GAATAGATGG ATTACCAGGG
    CAACAAGGCC TCCCTGGGCT TCCAGGAATC ACCTTGCCGT GTATAATTCC TGAGACATAT
    GGTCTGTCTG GCTTACCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGACCC CCGGTCAGCC TGGATTGCGT GGGAATAAAG GAGAGCCCGG AAGTCCAGGA
    TTAGTTCACC TTCCTGAATT GCCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT
    GGGTTTCCTG GACTCCCTGG AAAAGATGGC TTGCCTGGGA AACTTGGCAG TCCGGGTTTA
    CCTGGTCCCA AGGGAGCCCC TGGTGACATC TTTGGTGCTG AGAATGGTTC TCCGGGGGAG
    CAAGGCCTAC AGGGATTACC AGGGGACAGA GGACTTCCTG GAGACTCTGG CCTTCCAGGA
    CCCAAGGGTT TGCTTGGAAA GTCGGGCCTG CTAGGCCCCA AAGGTGAACG GGGCAGCCCT
    GGAACACCGG GCCACATGGG AGAGCCAGGG CCCCCAGGGC CTGATGGTCT ATACGGCAGC
    ATCAAGGGCA AGCCAGGGCT CCCTGGAGTG CCAGGCTTTC CAGGCCCTTC AGGACATCCT
    GGAAAGAAAG GTTTAAGAGG CGAGACAGGT TCCCCTGCAT CAGCTGGAAA GAGAGGCCTG
    CCAGGGCTGA AAGGCCTTCC GGGATCTCCA GGGCTGATTG GCTTCTTGGG GAGCTCAGGC
    TTGCCAGGGA ACACTGGGTT GCCAGGCCTG CCAGGTCCAA AGGGTGAAAA GGGGTCTGTT
    GGGCTGGTAG GTTTTCCAGG GATGCCAGGT CTCCCTGGTA TTCCTGGCAC GAGTGGAATA
    AAGGGAATTT CTGGGTCAGC CGGAAGAGTG GGACCATCTG GAAAGGCTGG CCATGCTGGT
    GAAAAAGGAG ACAGAGGCGA TCCAGGGCCA GCTGGAGTAC CCAGTCCAAG ACCTCCCATG
    CTGAACCTCC AGTTCAAAGG AGACAAAGGC TCTAGAGGCT CAGCTGGATT GGATGGATTT
    CCCGGGCCCA GAGGTGACAA AGGAGAGGCC GGTCCCCTGG GGCCACCAGG CTTGCCTGGA
    GCTCCTGGCT TCCCCAGTAC CATCAAGGGG CTTATTGGGA GGGCAGGCCT CCCTGGCTCC
    ATAGGACTCC GGGGCTTACC TGGCCTGAAG GGGTCCCCTG GAATCACAGG CTTCCCGGGA
    ATAGCAGGAG AAAGCGGTTC ACAGGGTCTC AATGGAGCTC CTGGACTCCC AGGAGCATCT
    GGTCTCCCAG GTTCAAAAGG AGATCAAGGT CAAACACTTG GAATTTCTGG TAGCCCAGGA
    CTCAAGGGGC AACCTGGGGA GTCTGGTTTT AAAGGCGTGC AAGGAACCAA TGGACTGGTT
    GGTGATGTGG GTCTCCCAGG AAGCAAAGGG GAAGATGGGA AAGTTGGTAT TTCTGGAGAC
    CTCGGCCTTC CTGGCTCCCC AGGACTCCCC GGGATTGCAG GCATGAGAGG AAATCCAGGG
    CTTCCGGGTT CTCCAGGCCA TCCAGGGGCA ACTGGGACCC TTGGACCCCC TGGCCTAATA
    GGAACCAAAG GTTTCCCTGG ACTTCCTGGT TTACATGGAC TCAATGGGCT TCCAGGAACC
    AAGGGAACAC ATGGAACTCC AGGACCTAGT ATAAGTGGTG TGCCGGGGCC AGCGGGCCTG
    CCTGGCCCCA AAGGAGAGAA AGGATCTCCA GGAACTGGTA TCGGAGCCCC AGGGAGGCCA
    GGCTCAAGAG GGCAAAAAGG TGGCCGAGGT TTCCCGGGTC TCCAGGGCCC TGCTGGTCTG
    CCTGGTGCCC CAGGCCTCTC CTTGCCTTCG ATCATAGTAG GACAGCCTGG CGACCCTGGG
    CGACCAGGGC TAGATGGAGA GCAAGGCCGC CCGGGCCCCC CGGGGCCCCC GGGGCCCCCT
    GGGCCATCCT CCAATCAAGG TGACCCTGGA GACCCTGGCT TCCCTGGAGT TCCTGGCCCT
    CAAGGGCCCA AAGGAGTCCA AGGAATTCCA GGTTTCTCCG GCCTCCCTGG AGAGCTAGGG
    CTAAAAGGCA TGAGAGGTGA GCCTGGCTTC ATGGGGATTC CAGGCAAGGT TGGGCCACCT
    GGAGACCCAG GACTTCCCGG GATGAAGGGG AAGGCAGGGC CCCAAGGCTT TTCCGGACCC
    CAAGGTGCTC CTGGACAGAC AACAATTGCA GAAGCTGTCC AGGTTCCTCC TGGACCCATG
    GGTCTACCGG GCATCGATGG CATCCCTGGC CTCACGGGAG ATCCTGGGGT TCAAGGTCCT
    GTAGGCCTCC AAGGCTCCAA AGGTTTACCT GGCGTCCCTG GCAAAGATGG TCTCAATGGA
    ATCCCCGGTC CGCCTGGGGC TCTTGGCGAC CCTGGTCTCC CTGGACTGCA AGGCCCTCCA
    GGATTTGAAG GAGCTCCAGG GAAGAAGGGT CCCTTCGGGA GGGCTGGAGT GCCTGGGCAG
    AGTGTGAGAG TGGGCTACAC CTTGGTGAAG CACAGCCAGT CAGAACAGCT GCCCCTGTGC
    CCTGCCGGGA TGAGCCAGCT GTGGGTGGGT TACAGCTTGC TGTTCGTGGA GGGCCAGGAG
    AAAGCCCACA ACCAGGACCT GGGTTTTGCT GGCTCCTGCC TGCCCCGCTT TAGCACCATG
    CCCTTCATCT ACTGCAACAT CAACGAAGTG TGCCACTACG CCAGACGCAA TGATAAATCC
    TACTGGCTCT CCACCACCGC CCCCATTCCC ATGATGCCCG TCGGCCAGGC CCAGATCCCC
    CAGTACATCA GCCGTTGTTC GGTGTGCGAG GCACCCTCAC AGGCCATTGC CGTGCACAGC
    CAGGACATCA CCATCCCGCA GTGCCCCCTG GGCTGGCACA GCCTCTGGAT TGGGTACTCC
    TTCCTCATGC ACACTGCCGC TGGTGCCGAG GGCGGCGGCC AGTCCCTGGT CTCACCGGGC
    TCCTGCCTGG AGGATTTCCG TGCTACTCCT TTCATTGAGT GCAGTGGGGC CCGGGGCACC
    TGCCACTACT TCGCCAACAA GTACAGTTTC TGGCTGACAA CCGTGGAAGA GAGGCAGCAG
    TTCCGGGAGG AACCAGTCTC TGAAACGCTC AAGGCTGGGC AGCTCCACAC CCGAGTCAGC
    CGCTGCCAGG TGTGTATGAG AAGCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32750
    Clone ID Related Accession (Same CDS sequence) XM_014111911.1 , XM_845861.4 , XM_014111911.2
    Accession Version XM_014111911.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-6(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111911.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACTGAGGA ACTGGCAGGA 
    GCCGGAGAGA AGAAGTCCTA TGGAAAGCCA TGTGGTGGCC AAGACTGCAG GGGAGGCTGT
    AAATGCTTTC CTGAGAAAGG AGCGAGAGGG CAACCAGGGC CAATCGGAAT TCAAGGTCCA
    ACGGGTCCTC AAGGATTTGC CGGCCCTAGT GGTTTAGCAG GGTTGAAAGG AGAAAGGGGC
    TCCCCAGGCC CCCTGGGACC ATATGGACCA AAAGGAGATA AGGGCCCCAT GGGAGTGCCT
    GGCTTTCTCG GCATCAATGG GATTCCGGGC CACCCTGGAC AGCCAGGGCC CAGAGGCCCA
    CCTGGCCTGG ATGGTTGTAA TGGAACTCAA GGAGCTGTTG GATATCCAGG CCCTAATGGC
    TATCCTGGGC TTCTCGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTACTTT CAAAGGAATG AAGGGGGAGC CTGGGCTTCC TGGACTGGAT
    GGAATAATTG GCCCTCCAGG AGCACCTGGG TCCCCAGGAG CCGTAGGACC TATGGGACCA
    CCAGGATTGC AAGGTCCTCC AGGCCCTCCT GGCTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCAGG CCCTGCAGGA
    CCCCCACCAT CCACTGGGGA ACTGGAATTT ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCTTCC GGGTTTCCCA GGAATAAGTG GCCCTCCAGG TCTCCCGGGA
    CTTGGAACTG CTGGAGAAAA GGGAGAAAAG GGAATCCCTG GTTTGCCAGG ACCAAGGGGT
    CCCATGGGTT TAGAAAGACT CCAAGGCCCT CCAGGGATTC AGGGCAAGAA GGGGTCCTCA
    GGTTTTCCTG GGCTTAATGG ACTCCCAGGA ATTAAGGGTG AAAAGGGTGG CATTGGCCTG
    CCAGGCCCAG ATGTGTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAT
    CCCGGTATGC CAGGTCTCCC AGGACTTAAA GGAGATGAAG GCATCCAGGG CCTGCCTGGC
    CCTTCTGGCA TCCCTGGCTT ACCAGCTTTA CCAGGTGTCC CAGGTGCTCT AGGCCCCCAG
    GGAGTTCCAG GTTTAAAGGG GGACCAAGGA AGCCCAGGCC GTACCACAGT TGGGACAGCT
    GGCACCCCAG GCAGAGATGG TTTGCCAGGC CTACCAGGCC TACGAGGCCC ACCCGGTCCA
    GCATTTGAGA CTGAAACCCT ACAGAACACA GAGCCAGGGT TCCCTGGTCT CCGAGGAGAA
    AGAGGTCCAA AAGGAAACCC AGGCCTCAAA GGCATAAAAG GAGATTTGGG CTTTTGTGCT
    TGTGACGGTG GGGTCCCTAA CACTGGACCA CCTGGGGAGC CAGGCATGCC TGGGCCACCA
    GGTCTCATAG GTCTTCCGGG CCTTAAAGGA ACCAGAGGAG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGCTCC AGGCTTATTT GGGCCTCCAG GTCCTGCGGG CTCCAAAGGA
    GATAAAGGGG AGCCAACTTT CAGTTCCGCA TCAGGGGTGC CAGGGGAGCA GGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG GGAGATGGGA GCTCCAGGCA AGGATGGAAT ACCAGGTCTA
    CCAGGTCTGC CAGGTCTTCC GGGTGACGGT GGACAGGGCT TCCCAGGTGA AAAGGGGTTA
    CCAGGACTGC CTGGTGAAAG GGGCCACAGT GGCCCAACTG GCCCCCCAGG AATTGGGCTG
    CCAGGATCTC CTGGACCTCG TGGGCTTCCT GGAGATCAAG GAATAGATGG ATTACCAGGG
    CAACAAGGCC TCCCTGGGCT TCCAGGAATC ACCTTGCCGT GTATAATTCC TGAGACATAT
    GGTCTGTCTG GCTTACCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGACCC CCGGTCAGCC TGGATTGCGT GGGAATAAAG GAGAGCCCGG AAGTCCAGGA
    TTAGTTCACC TTCCTGAATT GCCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT
    GGGTTTCCTG GACTCCCTGG AAAAGATGGC TTGCCTGGGA AACTTGGCAG TCCGGGTTTA
    CCTGGTCCCA AGGGAGCCCC TGGTGACATC TTTGGTGCTG AGAATGGTTC TCCGGGGGAG
    CAAGGCCTAC AGGGATTACC AGGGGACAGA GGACTTCCTG GAGACTCTGG CCTTCCAGGA
    CCCAAGGGTT TGCTTGGAAA GTCGGGCCTG CTAGGCCCCA AAGGTGAACG GGGCAGCCCT
    GGAACACCGG GCCACATGGG AGAGCCAGGG CCCCCAGGGC CTGATGGTCT ATACGGCAGC
    ATCAAGGGCA AGCCAGGGCT CCCTGGAGTG CCAGGCTTTC CAGGCCCTTC AGGACATCCT
    GGAAAGAAAG GTTTAAGAGG CGAGACAGGT TCCCCTGCAT CAGCTGGAAA GAGAGGCCTG
    CCAGGGCTGA AAGGCCTTCC GGGATCTCCA GGGCTGATTG GCTTCTTGGG GAGCTCAGGC
    TTGCCAGGGA ACACTGGGTT GCCAGGCCTG CCAGGTCCAA AGGGTGAAAA GGGGTCTGTT
    GGGCTGGTAG GTTTTCCAGG GATGCCAGGT CTCCCTGGTA TTCCTGGCAC GAGTGGAATA
    AAGGGAATTT CTGGGTCAGC CGGAAGAGTG GGACCATCTG GAAAGGCTGG CCATGCTGGT
    GAAAAAGGAG ACAGAGGCGA TCCAGGGCCA GCTGGAGTAC CCAGTCCAAG ACCTCCCATG
    CTGAACCTCC AGTTCAAAGG AGACAAAGGC TCTAGAGGCT CAGCTGGATT GGATGGATTT
    CCCGGGCCCA GAGGTGACAA AGGAGAGGCC GGTCCCCTGG GGCCACCAGG CTTGCCTGGA
    GCTCCTGGCT TCCCCAGTAC CATCAAGGGG CTTATTGGGA GGGCAGGCCT CCCTGGCTCC
    ATAGGACTCC GGGGCTTACC TGGCCTGAAG GGGTCCCCTG GAATCACAGG CTTCCCGGGA
    ATAGCAGGAG AAAGCGGTTC ACAGGGTCTC AATGGAGCTC CTGGACTCCC AGGAGCATCT
    GGTCTCCCAG GTTCAAAAGG AGATCAAGGT CAAACACTTG GAATTTCTGG TAGCCCAGGA
    CTCAAGGGGC AACCTGGGGA GTCTGGTTTT AAAGGCGTGC AAGGAACCAA TGGACTGGTT
    GGTGATGTGG GTCTCCCAGG AAGCAAAGGG GAAGATGGGA AAGTTGGTAT TTCTGGAGAC
    CTCGGCCTTC CTGGCTCCCC AGGACTCCCC GGGATTGCAG GCATGAGAGG AAATCCAGGG
    CTTCCGGGTT CTCCAGGCCA TCCAGGGGCA ACTGGGACCC TTGGACCCCC TGGCCTAATA
    GGAACCAAAG GTTTCCCTGG ACTTCCTGGT TTACATGGAC TCAATGGGCT TCCAGGAACC
    AAGGGAACAC ATGGAACTCC AGGACCTAGT ATAAGTGGTG TGCCGGGGCC AGCGGGCCTG
    CCTGGCCCCA AAGGAGAGAA AGGATCTCCA GGAACTGGTA TCGGAGCCCC AGGGAGGCCA
    GGCTCAAGAG GGCAAAAAGG TGGCCGAGGT TTCCCGGGTC TCCAGGGCCC TGCTGGTCTG
    CCTGGTGCCC CAGGCCTCTC CTTGCCTTCG ATCATAGTAG GACAGCCTGG CGACCCTGGG
    CGACCAGGGC TAGATGGAGA GCAAGGCCGC CCGGGCCCCC CGGGGCCCCC GGGGCCCCCT
    GGGCCATCCT CCAATCAAGG TGACCCTGGA GACCCTGGCT TCCCTGGAGT TCCTGGCCCT
    CAAGGGCCCA AAGGAGTCCA AGGAATTCCA GGTTTCTCCG GCCTCCCTGG AGAGCTAGGG
    CTAAAAGGCA TGAGAGGTGA GCCTGGCTTC ATGGGGATTC CAGGCAAGGT TGGGCCACCT
    GGAGACCCAG GACTTCCCGG GATGAAGGGG AAGGCAGGGC CCCAAGGCTT TTCCGGACCC
    CAAGGTGCTC CTGGACAGAC AACAATTGCA GAAGCTGTCC AGGTTCCTCC TGGACCCATG
    GGTCTACCGG GCATCGATGG CATCCCTGGC CTCACGGGAG ATCCTGGGGT TCAAGGTCCT
    GTAGGCCTCC AAGGCTCCAA AGGTTTACCT GGCGTCCCTG GCAAAGATGG TCTCAATGGA
    ATCCCCGGTC CGCCTGGGGC TCTTGGCGAC CCTGGTCTCC CTGGACTGCA AGGCCCTCCA
    GGATTTGAAG GAGCTCCAGG GAAGAAGGGT CCCTTCGGGA GGGCTGGAGT GCCTGGGCAG
    AGTGTGAGAG TGGGCTACAC CTTGGTGAAG CACAGCCAGT CAGAACAGCT GCCCCTGTGC
    CCTGCCGGGA TGAGCCAGCT GTGGGTGGGT TACAGCTTGC TGTTCGTGGA GGGCCAGGAG
    AAAGCCCACA ACCAGGACCT GGGTTTTGCT GGCTCCTGCC TGCCCCGCTT TAGCACCATG
    CCCTTCATCT ACTGCAACAT CAACGAAGTG TGCCACTACG CCAGACGCAA TGATAAATCC
    TACTGGCTCT CCACCACCGC CCCCATTCCC ATGATGCCCG TCGGCCAGGC CCAGATCCCC
    CAGTACATCA GCCGTTGTTC GGTGTGCGAG GCACCCTCAC AGGCCATTGC CGTGCACAGC
    CAGGACATCA CCATCCCGCA GTGCCCCCTG GGCTGGCACA GCCTCTGGAT TGGGTACTCC
    TTCCTCATGC ACACTGCCGC TGGTGCCGAG GGCGGCGGCC AGTCCCTGGT CTCACCGGGC
    TCCTGCCTGG AGGATTTCCG TGCTACTCCT TTCATTGAGT GCAGTGGGGC CCGGGGCACC
    TGCCACTACT TCGCCAACAA GTACAGTTTC TGGCTGACAA CCGTGGAAGA GAGGCAGCAG
    TTCCGGGAGG AACCAGTCTC TGAAACGCTC AAGGCTGGGC AGCTCCACAC CCGAGTCAGC
    CGCTGCCAGG TGTGTATGAG AAGCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967386.1
    CDS505..5574
    Translation

    Target ORF information:

    RefSeq Version XM_014111911.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type IV alpha 6 chain (COL4A6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111911.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACTGAGGA ACTGGCAGGA 
    GCCGGAGAGA AGAAGTCCTA TGGAAAGCCA TGTGGTGGCC AAGACTGCAG GGGAGGCTGT
    AAATGCTTTC CTGAGAAAGG AGCGAGAGGG CAACCAGGGC CAATCGGAAT TCAAGGTCCA
    ACGGGTCCTC AAGGATTTGC CGGCCCTAGT GGTTTAGCAG GGTTGAAAGG AGAAAGGGGC
    TCCCCAGGCC CCCTGGGACC ATATGGACCA AAAGGAGATA AGGGCCCCAT GGGAGTGCCT
    GGCTTTCTCG GCATCAATGG GATTCCGGGC CACCCTGGAC AGCCAGGGCC CAGAGGCCCA
    CCTGGCCTGG ATGGTTGTAA TGGAACTCAA GGAGCTGTTG GATATCCAGG CCCTAATGGC
    TATCCTGGGC TTCTCGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTACTTT CAAAGGAATG AAGGGGGAGC CTGGGCTTCC TGGACTGGAT
    GGAATAATTG GCCCTCCAGG AGCACCTGGG TCCCCAGGAG CCGTAGGACC TATGGGACCA
    CCAGGATTGC AAGGTCCTCC AGGCCCTCCT GGCTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCAGG CCCTGCAGGA
    CCCCCACCAT CCACTGGGGA ACTGGAATTT ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCTTCC GGGTTTCCCA GGAATAAGTG GCCCTCCAGG TCTCCCGGGA
    CTTGGAACTG CTGGAGAAAA GGGAGAAAAG GGAATCCCTG GTTTGCCAGG ACCAAGGGGT
    CCCATGGGTT TAGAAAGACT CCAAGGCCCT CCAGGGATTC AGGGCAAGAA GGGGTCCTCA
    GGTTTTCCTG GGCTTAATGG ACTCCCAGGA ATTAAGGGTG AAAAGGGTGG CATTGGCCTG
    CCAGGCCCAG ATGTGTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAT
    CCCGGTATGC CAGGTCTCCC AGGACTTAAA GGAGATGAAG GCATCCAGGG CCTGCCTGGC
    CCTTCTGGCA TCCCTGGCTT ACCAGCTTTA CCAGGTGTCC CAGGTGCTCT AGGCCCCCAG
    GGAGTTCCAG GTTTAAAGGG GGACCAAGGA AGCCCAGGCC GTACCACAGT TGGGACAGCT
    GGCACCCCAG GCAGAGATGG TTTGCCAGGC CTACCAGGCC TACGAGGCCC ACCCGGTCCA
    GCATTTGAGA CTGAAACCCT ACAGAACACA GAGCCAGGGT TCCCTGGTCT CCGAGGAGAA
    AGAGGTCCAA AAGGAAACCC AGGCCTCAAA GGCATAAAAG GAGATTTGGG CTTTTGTGCT
    TGTGACGGTG GGGTCCCTAA CACTGGACCA CCTGGGGAGC CAGGCATGCC TGGGCCACCA
    GGTCTCATAG GTCTTCCGGG CCTTAAAGGA ACCAGAGGAG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGCTCC AGGCTTATTT GGGCCTCCAG GTCCTGCGGG CTCCAAAGGA
    GATAAAGGGG AGCCAACTTT CAGTTCCGCA TCAGGGGTGC CAGGGGAGCA GGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG GGAGATGGGA GCTCCAGGCA AGGATGGAAT ACCAGGTCTA
    CCAGGTCTGC CAGGTCTTCC GGGTGACGGT GGACAGGGCT TCCCAGGTGA AAAGGGGTTA
    CCAGGACTGC CTGGTGAAAG GGGCCACAGT GGCCCAACTG GCCCCCCAGG AATTGGGCTG
    CCAGGATCTC CTGGACCTCG TGGGCTTCCT GGAGATCAAG GAATAGATGG ATTACCAGGG
    CAACAAGGCC TCCCTGGGCT TCCAGGAATC ACCTTGCCGT GTATAATTCC TGAGACATAT
    GGTCTGTCTG GCTTACCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGACCC CCGGTCAGCC TGGATTGCGT GGGAATAAAG GAGAGCCCGG AAGTCCAGGA
    TTAGTTCACC TTCCTGAATT GCCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT
    GGGTTTCCTG GACTCCCTGG AAAAGATGGC TTGCCTGGGA AACTTGGCAG TCCGGGTTTA
    CCTGGTCCCA AGGGAGCCCC TGGTGACATC TTTGGTGCTG AGAATGGTTC TCCGGGGGAG
    CAAGGCCTAC AGGGATTACC AGGGGACAGA GGACTTCCTG GAGACTCTGG CCTTCCAGGA
    CCCAAGGGTT TGCTTGGAAA GTCGGGCCTG CTAGGCCCCA AAGGTGAACG GGGCAGCCCT
    GGAACACCGG GCCACATGGG AGAGCCAGGG CCCCCAGGGC CTGATGGTCT ATACGGCAGC
    ATCAAGGGCA AGCCAGGGCT CCCTGGAGTG CCAGGCTTTC CAGGCCCTTC AGGACATCCT
    GGAAAGAAAG GTTTAAGAGG CGAGACAGGT TCCCCTGCAT CAGCTGGAAA GAGAGGCCTG
    CCAGGGCTGA AAGGCCTTCC GGGATCTCCA GGGCTGATTG GCTTCTTGGG GAGCTCAGGC
    TTGCCAGGGA ACACTGGGTT GCCAGGCCTG CCAGGTCCAA AGGGTGAAAA GGGGTCTGTT
    GGGCTGGTAG GTTTTCCAGG GATGCCAGGT CTCCCTGGTA TTCCTGGCAC GAGTGGAATA
    AAGGGAATTT CTGGGTCAGC CGGAAGAGTG GGACCATCTG GAAAGGCTGG CCATGCTGGT
    GAAAAAGGAG ACAGAGGCGA TCCAGGGCCA GCTGGAGTAC CCAGTCCAAG ACCTCCCATG
    CTGAACCTCC AGTTCAAAGG AGACAAAGGC TCTAGAGGCT CAGCTGGATT GGATGGATTT
    CCCGGGCCCA GAGGTGACAA AGGAGAGGCC GGTCCCCTGG GGCCACCAGG CTTGCCTGGA
    GCTCCTGGCT TCCCCAGTAC CATCAAGGGG CTTATTGGGA GGGCAGGCCT CCCTGGCTCC
    ATAGGACTCC GGGGCTTACC TGGCCTGAAG GGGTCCCCTG GAATCACAGG CTTCCCGGGA
    ATAGCAGGAG AAAGCGGTTC ACAGGGTCTC AATGGAGCTC CTGGACTCCC AGGAGCATCT
    GGTCTCCCAG GTTCAAAAGG AGATCAAGGT CAAACACTTG GAATTTCTGG TAGCCCAGGA
    CTCAAGGGGC AACCTGGGGA GTCTGGTTTT AAAGGCGTGC AAGGAACCAA TGGACTGGTT
    GGTGATGTGG GTCTCCCAGG AAGCAAAGGG GAAGATGGGA AAGTTGGTAT TTCTGGAGAC
    CTCGGCCTTC CTGGCTCCCC AGGACTCCCC GGGATTGCAG GCATGAGAGG AAATCCAGGG
    CTTCCGGGTT CTCCAGGCCA TCCAGGGGCA ACTGGGACCC TTGGACCCCC TGGCCTAATA
    GGAACCAAAG GTTTCCCTGG ACTTCCTGGT TTACATGGAC TCAATGGGCT TCCAGGAACC
    AAGGGAACAC ATGGAACTCC AGGACCTAGT ATAAGTGGTG TGCCGGGGCC AGCGGGCCTG
    CCTGGCCCCA AAGGAGAGAA AGGATCTCCA GGAACTGGTA TCGGAGCCCC AGGGAGGCCA
    GGCTCAAGAG GGCAAAAAGG TGGCCGAGGT TTCCCGGGTC TCCAGGGCCC TGCTGGTCTG
    CCTGGTGCCC CAGGCCTCTC CTTGCCTTCG ATCATAGTAG GACAGCCTGG CGACCCTGGG
    CGACCAGGGC TAGATGGAGA GCAAGGCCGC CCGGGCCCCC CGGGGCCCCC GGGGCCCCCT
    GGGCCATCCT CCAATCAAGG TGACCCTGGA GACCCTGGCT TCCCTGGAGT TCCTGGCCCT
    CAAGGGCCCA AAGGAGTCCA AGGAATTCCA GGTTTCTCCG GCCTCCCTGG AGAGCTAGGG
    CTAAAAGGCA TGAGAGGTGA GCCTGGCTTC ATGGGGATTC CAGGCAAGGT TGGGCCACCT
    GGAGACCCAG GACTTCCCGG GATGAAGGGG AAGGCAGGGC CCCAAGGCTT TTCCGGACCC
    CAAGGTGCTC CTGGACAGAC AACAATTGCA GAAGCTGTCC AGGTTCCTCC TGGACCCATG
    GGTCTACCGG GCATCGATGG CATCCCTGGC CTCACGGGAG ATCCTGGGGT TCAAGGTCCT
    GTAGGCCTCC AAGGCTCCAA AGGTTTACCT GGCGTCCCTG GCAAAGATGG TCTCAATGGA
    ATCCCCGGTC CGCCTGGGGC TCTTGGCGAC CCTGGTCTCC CTGGACTGCA AGGCCCTCCA
    GGATTTGAAG GAGCTCCAGG GAAGAAGGGT CCCTTCGGGA GGGCTGGAGT GCCTGGGCAG
    AGTGTGAGAG TGGGCTACAC CTTGGTGAAG CACAGCCAGT CAGAACAGCT GCCCCTGTGC
    CCTGCCGGGA TGAGCCAGCT GTGGGTGGGT TACAGCTTGC TGTTCGTGGA GGGCCAGGAG
    AAAGCCCACA ACCAGGACCT GGGTTTTGCT GGCTCCTGCC TGCCCCGCTT TAGCACCATG
    CCCTTCATCT ACTGCAACAT CAACGAAGTG TGCCACTACG CCAGACGCAA TGATAAATCC
    TACTGGCTCT CCACCACCGC CCCCATTCCC ATGATGCCCG TCGGCCAGGC CCAGATCCCC
    CAGTACATCA GCCGTTGTTC GGTGTGCGAG GCACCCTCAC AGGCCATTGC CGTGCACAGC
    CAGGACATCA CCATCCCGCA GTGCCCCCTG GGCTGGCACA GCCTCTGGAT TGGGTACTCC
    TTCCTCATGC ACACTGCCGC TGGTGCCGAG GGCGGCGGCC AGTCCCTGGT CTCACCGGGC
    TCCTGCCTGG AGGATTTCCG TGCTACTCCT TTCATTGAGT GCAGTGGGGC CCGGGGCACC
    TGCCACTACT TCGCCAACAA GTACAGTTTC TGGCTGACAA CCGTGGAAGA GAGGCAGCAG
    TTCCGGGAGG AACCAGTCTC TGAAACGCTC AAGGCTGGGC AGCTCCACAC CCGAGTCAGC
    CGCTGCCAGG TGTGTATGAG AAGCCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32751
    Clone ID Related Accession (Same CDS sequence) XM_014111912.1 , XM_014111912.2
    Accession Version XM_014111912.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product collagen alpha-6(IV) chain isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACTGAGGA ACTGGCAGGA 
    GCCGGAGAGA AGAAGTCCTA TGGAAAGCCA TGTGGTGGCC AAGACTGCAG GGGAGGCTGT
    AAATGCTTTC CTGAGAAAGG AGCGAGAGGG CAACCAGGGC CAATCGGAAT TCAAGGTCCA
    ACGGGTCCTC AAGGATTTGC CGGCCCTAGT GGTTTAGCAG GGTTGAAAGG AGAAAGGGGC
    TCCCCAGGCC CCCTGGGACC ATATGGACCA AAAGGAGATA AGGGCCCCAT GGGAGTGCCT
    GGCTTTCTCG GCATCAATGG GATTCCGGGC CACCCTGGAC AGCCAGGGCC CAGAGGCCCA
    CCTGGCCTGG ATGGTTGTAA TGGAACTCAA GGAGCTGTTG GATATCCAGG CCCTAATGGC
    TATCCTGGGC TTCTCGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTACTTT CAAAGGAATG AAGGGGGAGC CTGGGCTTCC TGGACTGGAT
    GGAATAATTG GCCCTCCAGG AGCACCTGGG TCCCCAGGAG CCGTAGGACC TATGGGACCA
    CCAGGATTGC AAGGTCCTCC AGGCCCTCCT GGCTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCAGG CCCTGCAGGA
    CCCCCACCAT CCACTGGGGA ACTGGAATTT ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCTTCC GGGTTTCCCA GGAATAAGTG GCCCTCCAGG TCTCCCGGGA
    CTTGGAACTG CTGGAGAAAA GGGAGAAAAG GGAATCCCTG GTTTGCCAGG ACCAAGGGGT
    CCCATGGGTT TAGAAAGACT CCAAGGCCCT CCAGGGATTC AGGGCAAGAA GGGGTCCTCA
    GGTTTTCCTG GGCTTAATGG ACTCCCAGGA ATTAAGGGTG AAAAGGGTGG CATTGGCCTG
    CCAGGCCCAG ATGTGTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAT
    CCCGGTATGC CAGGTCTCCC AGGACTTAAA GGAGATGAAG GCATCCAGGG CCTGCCTGGC
    CCTTCTGGCA TCCCTGGCTT ACCAGCTTTA CCAGGTGTCC CAGGTGCTCT AGGCCCCCAG
    GGAGTTCCAG GTTTAAAGGG GGACCAAGGA AGCCCAGGCC GTACCACAGT TGGGACAGCT
    GGCACCCCAG GCAGAGATGG TTTGCCAGGC CTACCAGGCC TACGAGGCCC ACCCGGTCCA
    GCATTTGAGA CTGAAACCCT ACAGAACACA GAGCCAGGGT TCCCTGGTCT CCGAGGAGAA
    AGAGGTCCAA AAGGAAACCC AGGCCTCAAA GGCATAAAAG GAGATTTGGG CTTTTGTGCT
    TGTGACGGTG GGGTCCCTAA CACTGGACCA CCTGGGGAGC CAGGCATGCC TGGGCCACCA
    GGTCTCATAG GTCTTCCGGG CCTTAAAGGA ACCAGAGGAG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGCTCC AGGCTTATTT GGGCCTCCAG GTCCTGCGGG CTCCAAAGGA
    GATAAAGGGG AGCCAACTTT CAGTTCCGCA TCAGGGGTGC CAGGGGAGCA GGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG GGAGATGGGA GCTCCAGGCA AGGATGGAAT ACCAGGTCTA
    CCAGGTCTGC CAGGTCTTCC GGGTGACGGT GGACAGGGCT TCCCAGGTGA AAAGGGGTTA
    CCAGGACTGC CTGGTGAAAG GGGCCACAGT GGCCCAACTG GCCCCCCAGG AATTGGGCTG
    CCAGGATCTC CTGGACCTCG TGGGCTTCCT GGAGATCAAG GAATAGATGG ATTACCAGGG
    CAACAAGGCC TCCCTGGGCT TCCAGGAATC ACCTTGCCGT GTATAATTCC TGAGACATAT
    GGTCTGTCTG GCTTACCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGACCC CCGGTCAGCC TGGATTGCGT GGGAATAAAG GAGAGCCCGG AAGTCCAGGA
    TTAGTTCACC TTCCTGAATT GCCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT
    GGGTTTCCTG GACTCCCTGG AAAAGATGGC TTGCCTGGGA AACTTGGCAG TCCGGGTTTA
    CCTGGTCCCA AGGGAGCCCC TGGTGACATC TTTGGTGCTG AGAATGGTTC TCCGGGGGAG
    CAAGGCCTAC AGGGATTACC AGGGGACAGA GGACTTCCTG GAGACTCTGG CCTTCCAGGA
    CCCAAGGGTT TGCTTGGAAA GTCGGGCCTG CTAGGCCCCA AAGGTGAACG GGGCAGCCCT
    GGAACACCGG GCCACATGGG AGAGCCAGGG CCCCCAGGGC CTGATGGTCT ATACGGCAGC
    ATCAAGGGCA AGCCAGGGCT CCCTGGAGTG CCAGGCTTTC CAGGCCCTTC AGGACATCCT
    GGAAAGAAAG GTTTAAGAGG CGAGACAGGT TCCCCTGCAT CAGCTGGAAA GAGAGGCCTG
    CCAGGGCTGA AAGGCCTTCC GGGATCTCCA GGGCTGATTG GCTTCTTGGG GAGCTCAGGC
    TTGCCAGGGA ACACTGGGTT GCCAGGCCTG CCAGGTCCAA AGGGTGAAAA GGGGTCTGTT
    GGGCTGGTAG GTTTTCCAGG GATGCCAGGT CTCCCTGGTA TTCCTGGCAC GAGTGGAATA
    AAGGGAATTT CTGGGTCAGC CGGAAGAGTG GGACCATCTG GAAAGGCTGG CCATGCTGGT
    GAAAAAGGAG ACAGAGGCGA TCCAGGGCCA GCTGGAGTAC CCAGTCCAAG ACCTCCCATG
    CTGAACCTCC AGTTCAAAGG AGACAAAGGC TCTAGAGGCT CAGCTGGATT GGATGGATTT
    CCCGGGCCCA GAGGTGACAA AGGAGAGGCC GGTCCCCTGG GGCCACCAGG CTTGCCTGGA
    GCTCCTGGCT TCCCCAGTAC CATCAAGGGG CTTATTGGGA GGGCAGGCCT CCCTGGCTCC
    ATAGGACTCC GGGGCTTACC TGGCCTGAAG GGGTCCCCTG GAATCACAGG CTTCCCGGGA
    ATAGCAGGAG AAAGCGGTTC ACAGGGTCTC AATGGAGCTC CTGGACTCCC AGGAGCATCT
    GGTCTCCCAG GTTCAAAAGG AGATCAAGGT CAAACACTTG GAATTTCTGG TAGCCCAGGA
    CTCAAGGGGC AACCTGGGGA GTCTGGTTTT AAAGGCGTGC AAGGAACCAA TGGACTGGTT
    GGTGATGTGG GTCTCCCAGG AAGCAAAGGG GAAGATGGGA AAGTTGGTAT TTCTGGAGAC
    CTCGGCCTTC CTGGCTCCCC AGGACTCCCC GGGATTGCAG GCATGAGAGG AAATCCAGGG
    CTTCCGGGTT CTCCAGGCCA TCCAGGGGCA ACTGGGACCC TTGGACCCCC TGGCCTAATA
    GGAACCAAAG GTTTCCCTGG ACTTCCTGGT TTACATGGAC TCAATGGGCT TCCAGGAACC
    AAGGGAACAC ATGGAACTCC AGGACCTAGT ATAAGTGGTG TGCCGGGGCC AGCGGGCCTG
    CCTGGCCCCA AAGGAGAGAA AGGATCTCCA GGAACTGGTA TCGGAGCCCC AGGGAGGCCA
    GGCTCAAGAG GGCAAAAAGG TGGCCGAGGT TTCCCGGGTC TCCAGGGCCC TGCTGGTCTG
    CCTGGTGCCC CAGGCCTCTC CTTGCCTTCG ATCATAGTAG GACAGCCTGG CGACCCTGGG
    CGACCAGGGC TAGATGGAGA GCAAGGTGAC CCTGGAGACC CTGGCTTCCC TGGAGTTCCT
    GGCCCTCAAG GGCCCAAAGG AGTCCAAGGA ATTCCAGGTT TCTCCGGCCT CCCTGGAGAG
    CTAGGGCTAA AAGGCATGAG AGGTGAGCCT GGCTTCATGG GGATTCCAGG CAAGGTTGGG
    CCACCTGGAG ACCCAGGACT TCCCGGGATG AAGGGGAAGG CAGGGCCCCA AGGCTTTTCC
    GGACCCCAAG GTGCTCCTGG ACAGACAACA ATTGCAGAAG CTGTCCAGGT TCCTCCTGGA
    CCCATGGGTC TACCGGGCAT CGATGGCATC CCTGGCCTCA CGGGAGATCC TGGGGTTCAA
    GGTCCTGTAG GCCTCCAAGG CTCCAAAGGT TTACCTGGCG TCCCTGGCAA AGATGGTCTC
    AATGGAATCC CCGGTCCGCC TGGGGCTCTT GGCGACCCTG GTCTCCCTGG ACTGCAAGGC
    CCTCCAGGAT TTGAAGGAGC TCCAGGGAAG AAGGGTCCCT TCGGGAGGGC TGGAGTGCCT
    GGGCAGAGTG TGAGAGTGGG CTACACCTTG GTGAAGCACA GCCAGTCAGA ACAGCTGCCC
    CTGTGCCCTG CCGGGATGAG CCAGCTGTGG GTGGGTTACA GCTTGCTGTT CGTGGAGGGC
    CAGGAGAAAG CCCACAACCA GGACCTGGGT TTTGCTGGCT CCTGCCTGCC CCGCTTTAGC
    ACCATGCCCT TCATCTACTG CAACATCAAC GAAGTGTGCC ACTACGCCAG ACGCAATGAT
    AAATCCTACT GGCTCTCCAC CACCGCCCCC ATTCCCATGA TGCCCGTCGG CCAGGCCCAG
    ATCCCCCAGT ACATCAGCCG TTGTTCGGTG TGCGAGGCAC CCTCACAGGC CATTGCCGTG
    CACAGCCAGG ACATCACCAT CCCGCAGTGC CCCCTGGGCT GGCACAGCCT CTGGATTGGG
    TACTCCTTCC TCATGCACAC TGCCGCTGGT GCCGAGGGCG GCGGCCAGTC CCTGGTCTCA
    CCGGGCTCCT GCCTGGAGGA TTTCCGTGCT ACTCCTTTCA TTGAGTGCAG TGGGGCCCGG
    GGCACCTGCC ACTACTTCGC CAACAAGTAC AGTTTCTGGC TGACAACCGT GGAAGAGAGG
    CAGCAGTTCC GGGAGGAACC AGTCTCTGAA ACGCTCAAGG CTGGGCAGCT CCACACCCGA
    GTCAGCCGCT GCCAGGTGTG TATGAGAAGC CTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967387.1
    CDS254..5269
    Translation

    Target ORF information:

    RefSeq Version XM_014111912.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris collagen, type IV, alpha 6 (COL4A6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111912.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACTGAGGA ACTGGCAGGA 
    GCCGGAGAGA AGAAGTCCTA TGGAAAGCCA TGTGGTGGCC AAGACTGCAG GGGAGGCTGT
    AAATGCTTTC CTGAGAAAGG AGCGAGAGGG CAACCAGGGC CAATCGGAAT TCAAGGTCCA
    ACGGGTCCTC AAGGATTTGC CGGCCCTAGT GGTTTAGCAG GGTTGAAAGG AGAAAGGGGC
    TCCCCAGGCC CCCTGGGACC ATATGGACCA AAAGGAGATA AGGGCCCCAT GGGAGTGCCT
    GGCTTTCTCG GCATCAATGG GATTCCGGGC CACCCTGGAC AGCCAGGGCC CAGAGGCCCA
    CCTGGCCTGG ATGGTTGTAA TGGAACTCAA GGAGCTGTTG GATATCCAGG CCCTAATGGC
    TATCCTGGGC TTCTCGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTACTTT CAAAGGAATG AAGGGGGAGC CTGGGCTTCC TGGACTGGAT
    GGAATAATTG GCCCTCCAGG AGCACCTGGG TCCCCAGGAG CCGTAGGACC TATGGGACCA
    CCAGGATTGC AAGGTCCTCC AGGCCCTCCT GGCTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCAGG CCCTGCAGGA
    CCCCCACCAT CCACTGGGGA ACTGGAATTT ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCTTCC GGGTTTCCCA GGAATAAGTG GCCCTCCAGG TCTCCCGGGA
    CTTGGAACTG CTGGAGAAAA GGGAGAAAAG GGAATCCCTG GTTTGCCAGG ACCAAGGGGT
    CCCATGGGTT TAGAAAGACT CCAAGGCCCT CCAGGGATTC AGGGCAAGAA GGGGTCCTCA
    GGTTTTCCTG GGCTTAATGG ACTCCCAGGA ATTAAGGGTG AAAAGGGTGG CATTGGCCTG
    CCAGGCCCAG ATGTGTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAT
    CCCGGTATGC CAGGTCTCCC AGGACTTAAA GGAGATGAAG GCATCCAGGG CCTGCCTGGC
    CCTTCTGGCA TCCCTGGCTT ACCAGCTTTA CCAGGTGTCC CAGGTGCTCT AGGCCCCCAG
    GGAGTTCCAG GTTTAAAGGG GGACCAAGGA AGCCCAGGCC GTACCACAGT TGGGACAGCT
    GGCACCCCAG GCAGAGATGG TTTGCCAGGC CTACCAGGCC TACGAGGCCC ACCCGGTCCA
    GCATTTGAGA CTGAAACCCT ACAGAACACA GAGCCAGGGT TCCCTGGTCT CCGAGGAGAA
    AGAGGTCCAA AAGGAAACCC AGGCCTCAAA GGCATAAAAG GAGATTTGGG CTTTTGTGCT
    TGTGACGGTG GGGTCCCTAA CACTGGACCA CCTGGGGAGC CAGGCATGCC TGGGCCACCA
    GGTCTCATAG GTCTTCCGGG CCTTAAAGGA ACCAGAGGAG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGCTCC AGGCTTATTT GGGCCTCCAG GTCCTGCGGG CTCCAAAGGA
    GATAAAGGGG AGCCAACTTT CAGTTCCGCA TCAGGGGTGC CAGGGGAGCA GGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG GGAGATGGGA GCTCCAGGCA AGGATGGAAT ACCAGGTCTA
    CCAGGTCTGC CAGGTCTTCC GGGTGACGGT GGACAGGGCT TCCCAGGTGA AAAGGGGTTA
    CCAGGACTGC CTGGTGAAAG GGGCCACAGT GGCCCAACTG GCCCCCCAGG AATTGGGCTG
    CCAGGATCTC CTGGACCTCG TGGGCTTCCT GGAGATCAAG GAATAGATGG ATTACCAGGG
    CAACAAGGCC TCCCTGGGCT TCCAGGAATC ACCTTGCCGT GTATAATTCC TGAGACATAT
    GGTCTGTCTG GCTTACCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGACCC CCGGTCAGCC TGGATTGCGT GGGAATAAAG GAGAGCCCGG AAGTCCAGGA
    TTAGTTCACC TTCCTGAATT GCCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT
    GGGTTTCCTG GACTCCCTGG AAAAGATGGC TTGCCTGGGA AACTTGGCAG TCCGGGTTTA
    CCTGGTCCCA AGGGAGCCCC TGGTGACATC TTTGGTGCTG AGAATGGTTC TCCGGGGGAG
    CAAGGCCTAC AGGGATTACC AGGGGACAGA GGACTTCCTG GAGACTCTGG CCTTCCAGGA
    CCCAAGGGTT TGCTTGGAAA GTCGGGCCTG CTAGGCCCCA AAGGTGAACG GGGCAGCCCT
    GGAACACCGG GCCACATGGG AGAGCCAGGG CCCCCAGGGC CTGATGGTCT ATACGGCAGC
    ATCAAGGGCA AGCCAGGGCT CCCTGGAGTG CCAGGCTTTC CAGGCCCTTC AGGACATCCT
    GGAAAGAAAG GTTTAAGAGG CGAGACAGGT TCCCCTGCAT CAGCTGGAAA GAGAGGCCTG
    CCAGGGCTGA AAGGCCTTCC GGGATCTCCA GGGCTGATTG GCTTCTTGGG GAGCTCAGGC
    TTGCCAGGGA ACACTGGGTT GCCAGGCCTG CCAGGTCCAA AGGGTGAAAA GGGGTCTGTT
    GGGCTGGTAG GTTTTCCAGG GATGCCAGGT CTCCCTGGTA TTCCTGGCAC GAGTGGAATA
    AAGGGAATTT CTGGGTCAGC CGGAAGAGTG GGACCATCTG GAAAGGCTGG CCATGCTGGT
    GAAAAAGGAG ACAGAGGCGA TCCAGGGCCA GCTGGAGTAC CCAGTCCAAG ACCTCCCATG
    CTGAACCTCC AGTTCAAAGG AGACAAAGGC TCTAGAGGCT CAGCTGGATT GGATGGATTT
    CCCGGGCCCA GAGGTGACAA AGGAGAGGCC GGTCCCCTGG GGCCACCAGG CTTGCCTGGA
    GCTCCTGGCT TCCCCAGTAC CATCAAGGGG CTTATTGGGA GGGCAGGCCT CCCTGGCTCC
    ATAGGACTCC GGGGCTTACC TGGCCTGAAG GGGTCCCCTG GAATCACAGG CTTCCCGGGA
    ATAGCAGGAG AAAGCGGTTC ACAGGGTCTC AATGGAGCTC CTGGACTCCC AGGAGCATCT
    GGTCTCCCAG GTTCAAAAGG AGATCAAGGT CAAACACTTG GAATTTCTGG TAGCCCAGGA
    CTCAAGGGGC AACCTGGGGA GTCTGGTTTT AAAGGCGTGC AAGGAACCAA TGGACTGGTT
    GGTGATGTGG GTCTCCCAGG AAGCAAAGGG GAAGATGGGA AAGTTGGTAT TTCTGGAGAC
    CTCGGCCTTC CTGGCTCCCC AGGACTCCCC GGGATTGCAG GCATGAGAGG AAATCCAGGG
    CTTCCGGGTT CTCCAGGCCA TCCAGGGGCA ACTGGGACCC TTGGACCCCC TGGCCTAATA
    GGAACCAAAG GTTTCCCTGG ACTTCCTGGT TTACATGGAC TCAATGGGCT TCCAGGAACC
    AAGGGAACAC ATGGAACTCC AGGACCTAGT ATAAGTGGTG TGCCGGGGCC AGCGGGCCTG
    CCTGGCCCCA AAGGAGAGAA AGGATCTCCA GGAACTGGTA TCGGAGCCCC AGGGAGGCCA
    GGCTCAAGAG GGCAAAAAGG TGGCCGAGGT TTCCCGGGTC TCCAGGGCCC TGCTGGTCTG
    CCTGGTGCCC CAGGCCTCTC CTTGCCTTCG ATCATAGTAG GACAGCCTGG CGACCCTGGG
    CGACCAGGGC TAGATGGAGA GCAAGGTGAC CCTGGAGACC CTGGCTTCCC TGGAGTTCCT
    GGCCCTCAAG GGCCCAAAGG AGTCCAAGGA ATTCCAGGTT TCTCCGGCCT CCCTGGAGAG
    CTAGGGCTAA AAGGCATGAG AGGTGAGCCT GGCTTCATGG GGATTCCAGG CAAGGTTGGG
    CCACCTGGAG ACCCAGGACT TCCCGGGATG AAGGGGAAGG CAGGGCCCCA AGGCTTTTCC
    GGACCCCAAG GTGCTCCTGG ACAGACAACA ATTGCAGAAG CTGTCCAGGT TCCTCCTGGA
    CCCATGGGTC TACCGGGCAT CGATGGCATC CCTGGCCTCA CGGGAGATCC TGGGGTTCAA
    GGTCCTGTAG GCCTCCAAGG CTCCAAAGGT TTACCTGGCG TCCCTGGCAA AGATGGTCTC
    AATGGAATCC CCGGTCCGCC TGGGGCTCTT GGCGACCCTG GTCTCCCTGG ACTGCAAGGC
    CCTCCAGGAT TTGAAGGAGC TCCAGGGAAG AAGGGTCCCT TCGGGAGGGC TGGAGTGCCT
    GGGCAGAGTG TGAGAGTGGG CTACACCTTG GTGAAGCACA GCCAGTCAGA ACAGCTGCCC
    CTGTGCCCTG CCGGGATGAG CCAGCTGTGG GTGGGTTACA GCTTGCTGTT CGTGGAGGGC
    CAGGAGAAAG CCCACAACCA GGACCTGGGT TTTGCTGGCT CCTGCCTGCC CCGCTTTAGC
    ACCATGCCCT TCATCTACTG CAACATCAAC GAAGTGTGCC ACTACGCCAG ACGCAATGAT
    AAATCCTACT GGCTCTCCAC CACCGCCCCC ATTCCCATGA TGCCCGTCGG CCAGGCCCAG
    ATCCCCCAGT ACATCAGCCG TTGTTCGGTG TGCGAGGCAC CCTCACAGGC CATTGCCGTG
    CACAGCCAGG ACATCACCAT CCCGCAGTGC CCCCTGGGCT GGCACAGCCT CTGGATTGGG
    TACTCCTTCC TCATGCACAC TGCCGCTGGT GCCGAGGGCG GCGGCCAGTC CCTGGTCTCA
    CCGGGCTCCT GCCTGGAGGA TTTCCGTGCT ACTCCTTTCA TTGAGTGCAG TGGGGCCCGG
    GGCACCTGCC ACTACTTCGC CAACAAGTAC AGTTTCTGGC TGACAACCGT GGAAGAGAGG
    CAGCAGTTCC GGGAGGAACC AGTCTCTGAA ACGCTCAAGG CTGGGCAGCT CCACACCCGA
    GTCAGCCGCT GCCAGGTGTG TATGAGAAGC CTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32751
    Clone ID Related Accession (Same CDS sequence) XM_014111912.1 , XM_014111912.2
    Accession Version XM_014111912.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product collagen alpha-6(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111912.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACTGAGGA ACTGGCAGGA 
    GCCGGAGAGA AGAAGTCCTA TGGAAAGCCA TGTGGTGGCC AAGACTGCAG GGGAGGCTGT
    AAATGCTTTC CTGAGAAAGG AGCGAGAGGG CAACCAGGGC CAATCGGAAT TCAAGGTCCA
    ACGGGTCCTC AAGGATTTGC CGGCCCTAGT GGTTTAGCAG GGTTGAAAGG AGAAAGGGGC
    TCCCCAGGCC CCCTGGGACC ATATGGACCA AAAGGAGATA AGGGCCCCAT GGGAGTGCCT
    GGCTTTCTCG GCATCAATGG GATTCCGGGC CACCCTGGAC AGCCAGGGCC CAGAGGCCCA
    CCTGGCCTGG ATGGTTGTAA TGGAACTCAA GGAGCTGTTG GATATCCAGG CCCTAATGGC
    TATCCTGGGC TTCTCGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTACTTT CAAAGGAATG AAGGGGGAGC CTGGGCTTCC TGGACTGGAT
    GGAATAATTG GCCCTCCAGG AGCACCTGGG TCCCCAGGAG CCGTAGGACC TATGGGACCA
    CCAGGATTGC AAGGTCCTCC AGGCCCTCCT GGCTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCAGG CCCTGCAGGA
    CCCCCACCAT CCACTGGGGA ACTGGAATTT ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCTTCC GGGTTTCCCA GGAATAAGTG GCCCTCCAGG TCTCCCGGGA
    CTTGGAACTG CTGGAGAAAA GGGAGAAAAG GGAATCCCTG GTTTGCCAGG ACCAAGGGGT
    CCCATGGGTT TAGAAAGACT CCAAGGCCCT CCAGGGATTC AGGGCAAGAA GGGGTCCTCA
    GGTTTTCCTG GGCTTAATGG ACTCCCAGGA ATTAAGGGTG AAAAGGGTGG CATTGGCCTG
    CCAGGCCCAG ATGTGTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAT
    CCCGGTATGC CAGGTCTCCC AGGACTTAAA GGAGATGAAG GCATCCAGGG CCTGCCTGGC
    CCTTCTGGCA TCCCTGGCTT ACCAGCTTTA CCAGGTGTCC CAGGTGCTCT AGGCCCCCAG
    GGAGTTCCAG GTTTAAAGGG GGACCAAGGA AGCCCAGGCC GTACCACAGT TGGGACAGCT
    GGCACCCCAG GCAGAGATGG TTTGCCAGGC CTACCAGGCC TACGAGGCCC ACCCGGTCCA
    GCATTTGAGA CTGAAACCCT ACAGAACACA GAGCCAGGGT TCCCTGGTCT CCGAGGAGAA
    AGAGGTCCAA AAGGAAACCC AGGCCTCAAA GGCATAAAAG GAGATTTGGG CTTTTGTGCT
    TGTGACGGTG GGGTCCCTAA CACTGGACCA CCTGGGGAGC CAGGCATGCC TGGGCCACCA
    GGTCTCATAG GTCTTCCGGG CCTTAAAGGA ACCAGAGGAG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGCTCC AGGCTTATTT GGGCCTCCAG GTCCTGCGGG CTCCAAAGGA
    GATAAAGGGG AGCCAACTTT CAGTTCCGCA TCAGGGGTGC CAGGGGAGCA GGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG GGAGATGGGA GCTCCAGGCA AGGATGGAAT ACCAGGTCTA
    CCAGGTCTGC CAGGTCTTCC GGGTGACGGT GGACAGGGCT TCCCAGGTGA AAAGGGGTTA
    CCAGGACTGC CTGGTGAAAG GGGCCACAGT GGCCCAACTG GCCCCCCAGG AATTGGGCTG
    CCAGGATCTC CTGGACCTCG TGGGCTTCCT GGAGATCAAG GAATAGATGG ATTACCAGGG
    CAACAAGGCC TCCCTGGGCT TCCAGGAATC ACCTTGCCGT GTATAATTCC TGAGACATAT
    GGTCTGTCTG GCTTACCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGACCC CCGGTCAGCC TGGATTGCGT GGGAATAAAG GAGAGCCCGG AAGTCCAGGA
    TTAGTTCACC TTCCTGAATT GCCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT
    GGGTTTCCTG GACTCCCTGG AAAAGATGGC TTGCCTGGGA AACTTGGCAG TCCGGGTTTA
    CCTGGTCCCA AGGGAGCCCC TGGTGACATC TTTGGTGCTG AGAATGGTTC TCCGGGGGAG
    CAAGGCCTAC AGGGATTACC AGGGGACAGA GGACTTCCTG GAGACTCTGG CCTTCCAGGA
    CCCAAGGGTT TGCTTGGAAA GTCGGGCCTG CTAGGCCCCA AAGGTGAACG GGGCAGCCCT
    GGAACACCGG GCCACATGGG AGAGCCAGGG CCCCCAGGGC CTGATGGTCT ATACGGCAGC
    ATCAAGGGCA AGCCAGGGCT CCCTGGAGTG CCAGGCTTTC CAGGCCCTTC AGGACATCCT
    GGAAAGAAAG GTTTAAGAGG CGAGACAGGT TCCCCTGCAT CAGCTGGAAA GAGAGGCCTG
    CCAGGGCTGA AAGGCCTTCC GGGATCTCCA GGGCTGATTG GCTTCTTGGG GAGCTCAGGC
    TTGCCAGGGA ACACTGGGTT GCCAGGCCTG CCAGGTCCAA AGGGTGAAAA GGGGTCTGTT
    GGGCTGGTAG GTTTTCCAGG GATGCCAGGT CTCCCTGGTA TTCCTGGCAC GAGTGGAATA
    AAGGGAATTT CTGGGTCAGC CGGAAGAGTG GGACCATCTG GAAAGGCTGG CCATGCTGGT
    GAAAAAGGAG ACAGAGGCGA TCCAGGGCCA GCTGGAGTAC CCAGTCCAAG ACCTCCCATG
    CTGAACCTCC AGTTCAAAGG AGACAAAGGC TCTAGAGGCT CAGCTGGATT GGATGGATTT
    CCCGGGCCCA GAGGTGACAA AGGAGAGGCC GGTCCCCTGG GGCCACCAGG CTTGCCTGGA
    GCTCCTGGCT TCCCCAGTAC CATCAAGGGG CTTATTGGGA GGGCAGGCCT CCCTGGCTCC
    ATAGGACTCC GGGGCTTACC TGGCCTGAAG GGGTCCCCTG GAATCACAGG CTTCCCGGGA
    ATAGCAGGAG AAAGCGGTTC ACAGGGTCTC AATGGAGCTC CTGGACTCCC AGGAGCATCT
    GGTCTCCCAG GTTCAAAAGG AGATCAAGGT CAAACACTTG GAATTTCTGG TAGCCCAGGA
    CTCAAGGGGC AACCTGGGGA GTCTGGTTTT AAAGGCGTGC AAGGAACCAA TGGACTGGTT
    GGTGATGTGG GTCTCCCAGG AAGCAAAGGG GAAGATGGGA AAGTTGGTAT TTCTGGAGAC
    CTCGGCCTTC CTGGCTCCCC AGGACTCCCC GGGATTGCAG GCATGAGAGG AAATCCAGGG
    CTTCCGGGTT CTCCAGGCCA TCCAGGGGCA ACTGGGACCC TTGGACCCCC TGGCCTAATA
    GGAACCAAAG GTTTCCCTGG ACTTCCTGGT TTACATGGAC TCAATGGGCT TCCAGGAACC
    AAGGGAACAC ATGGAACTCC AGGACCTAGT ATAAGTGGTG TGCCGGGGCC AGCGGGCCTG
    CCTGGCCCCA AAGGAGAGAA AGGATCTCCA GGAACTGGTA TCGGAGCCCC AGGGAGGCCA
    GGCTCAAGAG GGCAAAAAGG TGGCCGAGGT TTCCCGGGTC TCCAGGGCCC TGCTGGTCTG
    CCTGGTGCCC CAGGCCTCTC CTTGCCTTCG ATCATAGTAG GACAGCCTGG CGACCCTGGG
    CGACCAGGGC TAGATGGAGA GCAAGGTGAC CCTGGAGACC CTGGCTTCCC TGGAGTTCCT
    GGCCCTCAAG GGCCCAAAGG AGTCCAAGGA ATTCCAGGTT TCTCCGGCCT CCCTGGAGAG
    CTAGGGCTAA AAGGCATGAG AGGTGAGCCT GGCTTCATGG GGATTCCAGG CAAGGTTGGG
    CCACCTGGAG ACCCAGGACT TCCCGGGATG AAGGGGAAGG CAGGGCCCCA AGGCTTTTCC
    GGACCCCAAG GTGCTCCTGG ACAGACAACA ATTGCAGAAG CTGTCCAGGT TCCTCCTGGA
    CCCATGGGTC TACCGGGCAT CGATGGCATC CCTGGCCTCA CGGGAGATCC TGGGGTTCAA
    GGTCCTGTAG GCCTCCAAGG CTCCAAAGGT TTACCTGGCG TCCCTGGCAA AGATGGTCTC
    AATGGAATCC CCGGTCCGCC TGGGGCTCTT GGCGACCCTG GTCTCCCTGG ACTGCAAGGC
    CCTCCAGGAT TTGAAGGAGC TCCAGGGAAG AAGGGTCCCT TCGGGAGGGC TGGAGTGCCT
    GGGCAGAGTG TGAGAGTGGG CTACACCTTG GTGAAGCACA GCCAGTCAGA ACAGCTGCCC
    CTGTGCCCTG CCGGGATGAG CCAGCTGTGG GTGGGTTACA GCTTGCTGTT CGTGGAGGGC
    CAGGAGAAAG CCCACAACCA GGACCTGGGT TTTGCTGGCT CCTGCCTGCC CCGCTTTAGC
    ACCATGCCCT TCATCTACTG CAACATCAAC GAAGTGTGCC ACTACGCCAG ACGCAATGAT
    AAATCCTACT GGCTCTCCAC CACCGCCCCC ATTCCCATGA TGCCCGTCGG CCAGGCCCAG
    ATCCCCCAGT ACATCAGCCG TTGTTCGGTG TGCGAGGCAC CCTCACAGGC CATTGCCGTG
    CACAGCCAGG ACATCACCAT CCCGCAGTGC CCCCTGGGCT GGCACAGCCT CTGGATTGGG
    TACTCCTTCC TCATGCACAC TGCCGCTGGT GCCGAGGGCG GCGGCCAGTC CCTGGTCTCA
    CCGGGCTCCT GCCTGGAGGA TTTCCGTGCT ACTCCTTTCA TTGAGTGCAG TGGGGCCCGG
    GGCACCTGCC ACTACTTCGC CAACAAGTAC AGTTTCTGGC TGACAACCGT GGAAGAGAGG
    CAGCAGTTCC GGGAGGAACC AGTCTCTGAA ACGCTCAAGG CTGGGCAGCT CCACACCCGA
    GTCAGCCGCT GCCAGGTGTG TATGAGAAGC CTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967387.1
    CDS509..5524
    Translation

    Target ORF information:

    RefSeq Version XM_014111912.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris collagen type IV alpha 6 chain (COL4A6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111912.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCACCCTG GGTTGTGGCT GCTCCTGGTT ACGTTGTGCC TGACTGAGGA ACTGGCAGGA 
    GCCGGAGAGA AGAAGTCCTA TGGAAAGCCA TGTGGTGGCC AAGACTGCAG GGGAGGCTGT
    AAATGCTTTC CTGAGAAAGG AGCGAGAGGG CAACCAGGGC CAATCGGAAT TCAAGGTCCA
    ACGGGTCCTC AAGGATTTGC CGGCCCTAGT GGTTTAGCAG GGTTGAAAGG AGAAAGGGGC
    TCCCCAGGCC CCCTGGGACC ATATGGACCA AAAGGAGATA AGGGCCCCAT GGGAGTGCCT
    GGCTTTCTCG GCATCAATGG GATTCCGGGC CACCCTGGAC AGCCAGGGCC CAGAGGCCCA
    CCTGGCCTGG ATGGTTGTAA TGGAACTCAA GGAGCTGTTG GATATCCAGG CCCTAATGGC
    TATCCTGGGC TTCTCGGACC ACCTGGGCTT CCTGGTCAGA AAGGCTCTAA AGGTGAACCT
    GTCCTTGCCC AAGGTACTTT CAAAGGAATG AAGGGGGAGC CTGGGCTTCC TGGACTGGAT
    GGAATAATTG GCCCTCCAGG AGCACCTGGG TCCCCAGGAG CCGTAGGACC TATGGGACCA
    CCAGGATTGC AAGGTCCTCC AGGCCCTCCT GGCTTCCCTG GTCCTGATGG GAATATGGGG
    TTAGGTTTCC AAGGAGAGAA AGGAGTCAAG GGGGATGTTG GCCTCCCAGG CCCTGCAGGA
    CCCCCACCAT CCACTGGGGA ACTGGAATTT ATGGGATTTC CCAAAGGGAA GAAAGGATCC
    AAGGGTGAAC CAGGGCTTCC GGGTTTCCCA GGAATAAGTG GCCCTCCAGG TCTCCCGGGA
    CTTGGAACTG CTGGAGAAAA GGGAGAAAAG GGAATCCCTG GTTTGCCAGG ACCAAGGGGT
    CCCATGGGTT TAGAAAGACT CCAAGGCCCT CCAGGGATTC AGGGCAAGAA GGGGTCCTCA
    GGTTTTCCTG GGCTTAATGG ACTCCCAGGA ATTAAGGGTG AAAAGGGTGG CATTGGCCTG
    CCAGGCCCAG ATGTGTTCAT CGATACAGAT GGTGCTGTGA TCTCAGGTTA TCCTGGAGAT
    CCCGGTATGC CAGGTCTCCC AGGACTTAAA GGAGATGAAG GCATCCAGGG CCTGCCTGGC
    CCTTCTGGCA TCCCTGGCTT ACCAGCTTTA CCAGGTGTCC CAGGTGCTCT AGGCCCCCAG
    GGAGTTCCAG GTTTAAAGGG GGACCAAGGA AGCCCAGGCC GTACCACAGT TGGGACAGCT
    GGCACCCCAG GCAGAGATGG TTTGCCAGGC CTACCAGGCC TACGAGGCCC ACCCGGTCCA
    GCATTTGAGA CTGAAACCCT ACAGAACACA GAGCCAGGGT TCCCTGGTCT CCGAGGAGAA
    AGAGGTCCAA AAGGAAACCC AGGCCTCAAA GGCATAAAAG GAGATTTGGG CTTTTGTGCT
    TGTGACGGTG GGGTCCCTAA CACTGGACCA CCTGGGGAGC CAGGCATGCC TGGGCCACCA
    GGTCTCATAG GTCTTCCGGG CCTTAAAGGA ACCAGAGGAG ATCCAGGCTC TGGGGGTGCA
    CAGGGCCCAT CAGGGGCTCC AGGCTTATTT GGGCCTCCAG GTCCTGCGGG CTCCAAAGGA
    GATAAAGGGG AGCCAACTTT CAGTTCCGCA TCAGGGGTGC CAGGGGAGCA GGGTGATCCT
    GGCCCCCAGG GGCTCCCTGG GGAGATGGGA GCTCCAGGCA AGGATGGAAT ACCAGGTCTA
    CCAGGTCTGC CAGGTCTTCC GGGTGACGGT GGACAGGGCT TCCCAGGTGA AAAGGGGTTA
    CCAGGACTGC CTGGTGAAAG GGGCCACAGT GGCCCAACTG GCCCCCCAGG AATTGGGCTG
    CCAGGATCTC CTGGACCTCG TGGGCTTCCT GGAGATCAAG GAATAGATGG ATTACCAGGG
    CAACAAGGCC TCCCTGGGCT TCCAGGAATC ACCTTGCCGT GTATAATTCC TGAGACATAT
    GGTCTGTCTG GCTTACCAGG AGCTCCCGGA TTCCCAGGCC CTAAAGGGGC CCGTGGCCTC
    CCTGGGACCC CCGGTCAGCC TGGATTGCGT GGGAATAAAG GAGAGCCCGG AAGTCCAGGA
    TTAGTTCACC TTCCTGAATT GCCAGGATTT CCTGGACCTC GTGGGGAGAA GGGCTTGCCT
    GGGTTTCCTG GACTCCCTGG AAAAGATGGC TTGCCTGGGA AACTTGGCAG TCCGGGTTTA
    CCTGGTCCCA AGGGAGCCCC TGGTGACATC TTTGGTGCTG AGAATGGTTC TCCGGGGGAG
    CAAGGCCTAC AGGGATTACC AGGGGACAGA GGACTTCCTG GAGACTCTGG CCTTCCAGGA
    CCCAAGGGTT TGCTTGGAAA GTCGGGCCTG CTAGGCCCCA AAGGTGAACG GGGCAGCCCT
    GGAACACCGG GCCACATGGG AGAGCCAGGG CCCCCAGGGC CTGATGGTCT ATACGGCAGC
    ATCAAGGGCA AGCCAGGGCT CCCTGGAGTG CCAGGCTTTC CAGGCCCTTC AGGACATCCT
    GGAAAGAAAG GTTTAAGAGG CGAGACAGGT TCCCCTGCAT CAGCTGGAAA GAGAGGCCTG
    CCAGGGCTGA AAGGCCTTCC GGGATCTCCA GGGCTGATTG GCTTCTTGGG GAGCTCAGGC
    TTGCCAGGGA ACACTGGGTT GCCAGGCCTG CCAGGTCCAA AGGGTGAAAA GGGGTCTGTT
    GGGCTGGTAG GTTTTCCAGG GATGCCAGGT CTCCCTGGTA TTCCTGGCAC GAGTGGAATA
    AAGGGAATTT CTGGGTCAGC CGGAAGAGTG GGACCATCTG GAAAGGCTGG CCATGCTGGT
    GAAAAAGGAG ACAGAGGCGA TCCAGGGCCA GCTGGAGTAC CCAGTCCAAG ACCTCCCATG
    CTGAACCTCC AGTTCAAAGG AGACAAAGGC TCTAGAGGCT CAGCTGGATT GGATGGATTT
    CCCGGGCCCA GAGGTGACAA AGGAGAGGCC GGTCCCCTGG GGCCACCAGG CTTGCCTGGA
    GCTCCTGGCT TCCCCAGTAC CATCAAGGGG CTTATTGGGA GGGCAGGCCT CCCTGGCTCC
    ATAGGACTCC GGGGCTTACC TGGCCTGAAG GGGTCCCCTG GAATCACAGG CTTCCCGGGA
    ATAGCAGGAG AAAGCGGTTC ACAGGGTCTC AATGGAGCTC CTGGACTCCC AGGAGCATCT
    GGTCTCCCAG GTTCAAAAGG AGATCAAGGT CAAACACTTG GAATTTCTGG TAGCCCAGGA
    CTCAAGGGGC AACCTGGGGA GTCTGGTTTT AAAGGCGTGC AAGGAACCAA TGGACTGGTT
    GGTGATGTGG GTCTCCCAGG AAGCAAAGGG GAAGATGGGA AAGTTGGTAT TTCTGGAGAC
    CTCGGCCTTC CTGGCTCCCC AGGACTCCCC GGGATTGCAG GCATGAGAGG AAATCCAGGG
    CTTCCGGGTT CTCCAGGCCA TCCAGGGGCA ACTGGGACCC TTGGACCCCC TGGCCTAATA
    GGAACCAAAG GTTTCCCTGG ACTTCCTGGT TTACATGGAC TCAATGGGCT TCCAGGAACC
    AAGGGAACAC ATGGAACTCC AGGACCTAGT ATAAGTGGTG TGCCGGGGCC AGCGGGCCTG
    CCTGGCCCCA AAGGAGAGAA AGGATCTCCA GGAACTGGTA TCGGAGCCCC AGGGAGGCCA
    GGCTCAAGAG GGCAAAAAGG TGGCCGAGGT TTCCCGGGTC TCCAGGGCCC TGCTGGTCTG
    CCTGGTGCCC CAGGCCTCTC CTTGCCTTCG ATCATAGTAG GACAGCCTGG CGACCCTGGG
    CGACCAGGGC TAGATGGAGA GCAAGGTGAC CCTGGAGACC CTGGCTTCCC TGGAGTTCCT
    GGCCCTCAAG GGCCCAAAGG AGTCCAAGGA ATTCCAGGTT TCTCCGGCCT CCCTGGAGAG
    CTAGGGCTAA AAGGCATGAG AGGTGAGCCT GGCTTCATGG GGATTCCAGG CAAGGTTGGG
    CCACCTGGAG ACCCAGGACT TCCCGGGATG AAGGGGAAGG CAGGGCCCCA AGGCTTTTCC
    GGACCCCAAG GTGCTCCTGG ACAGACAACA ATTGCAGAAG CTGTCCAGGT TCCTCCTGGA
    CCCATGGGTC TACCGGGCAT CGATGGCATC CCTGGCCTCA CGGGAGATCC TGGGGTTCAA
    GGTCCTGTAG GCCTCCAAGG CTCCAAAGGT TTACCTGGCG TCCCTGGCAA AGATGGTCTC
    AATGGAATCC CCGGTCCGCC TGGGGCTCTT GGCGACCCTG GTCTCCCTGG ACTGCAAGGC
    CCTCCAGGAT TTGAAGGAGC TCCAGGGAAG AAGGGTCCCT TCGGGAGGGC TGGAGTGCCT
    GGGCAGAGTG TGAGAGTGGG CTACACCTTG GTGAAGCACA GCCAGTCAGA ACAGCTGCCC
    CTGTGCCCTG CCGGGATGAG CCAGCTGTGG GTGGGTTACA GCTTGCTGTT CGTGGAGGGC
    CAGGAGAAAG CCCACAACCA GGACCTGGGT TTTGCTGGCT CCTGCCTGCC CCGCTTTAGC
    ACCATGCCCT TCATCTACTG CAACATCAAC GAAGTGTGCC ACTACGCCAG ACGCAATGAT
    AAATCCTACT GGCTCTCCAC CACCGCCCCC ATTCCCATGA TGCCCGTCGG CCAGGCCCAG
    ATCCCCCAGT ACATCAGCCG TTGTTCGGTG TGCGAGGCAC CCTCACAGGC CATTGCCGTG
    CACAGCCAGG ACATCACCAT CCCGCAGTGC CCCCTGGGCT GGCACAGCCT CTGGATTGGG
    TACTCCTTCC TCATGCACAC TGCCGCTGGT GCCGAGGGCG GCGGCCAGTC CCTGGTCTCA
    CCGGGCTCCT GCCTGGAGGA TTTCCGTGCT ACTCCTTTCA TTGAGTGCAG TGGGGCCCGG
    GGCACCTGCC ACTACTTCGC CAACAAGTAC AGTTTCTGGC TGACAACCGT GGAAGAGAGG
    CAGCAGTTCC GGGAGGAACC AGTCTCTGAA ACGCTCAAGG CTGGGCAGCT CCACACCCGA
    GTCAGCCGCT GCCAGGTGTG TATGAGAAGC CTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.