GUSB cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUSB
Entrez Gene ID	403831
Full Name	glucuronidase beta
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014114125.2	XP_013969600.1	beta-glucuronidase isoform X2
XM_014114125.1	XP_013969600.1	beta-glucuronidase isoform X2
NM_001003191.1	NP_001003191.1	beta-glucuronidase precursor
XM_014114126.1	XP_013969601.1	beta-glucuronidase isoform X3
XM_014114126.2	XP_013969601.1	beta-glucuronidase isoform X3
XM_005620897.2	XP_005620954.1	beta-glucuronidase isoform X1
XM_005620897.3	XP_005620954.1	beta-glucuronidase isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gusb	NP_034498.1
Rattus norvegicus (Norway rat)	Gusb	NP_058711.2
Drosophila melanogaster (fruit fly)	CG15117	NP_725851.1
Caenorhabditis elegans (roundworm)	CELE_Y105E8B.9	NP_493548.1
Bos taurus (cattle)	GUSB	NP_001076905.1
Pan troglodytes (chimpanzee)	GUSB	XP_001138883.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011859	XP_320660.4
Homo sapiens (human)	GUSB	NP_000172.2
Canis lupus familiaris (dog)	GUSB	NP_001003191.1
Gallus gallus (chicken)	GUSB	NP_001034405.1
Danio rerio (zebrafish)	LOC100535236	XP_005165070.1
Danio rerio (zebrafish)	gusb	XP_700122.6
Xenopus tropicalis (tropical clawed frog)	gusb	XP_002935849.2

Gene Ontology
Bibliography

Related articles in PubMed

A novel GUSB mutation in Brazilian terriers with severe skeletal abnormalities defines the disease as mucopolysaccharidosis VII.
Hyt?nen MK, Arumilli M, Lappalainen AK, Kallio H, Snellman M, Sainio K, Lohi H
PloS one7(7)e40281(2012)

Construction and characterization of an eightfold redundant dog genomic bacterial artificial chromosome library.
Li R, Mignot E, Faraco J, Kadotani H, Cantanese J, Zhao B, Lin X, Hinton L, Ostrander EA, Patterson DF, de Jong PJ
Genomics58(1)9-17(1999 May)

Cloning of the canine beta-glucuronidase cDNA, mutation identification in canine MPS VII, and retroviral vector-mediated correction of MPS VII cells.
Ray J, Bouvet A, DeSanto C, Fyfe JC, Xu D, Wolfe JH, Aguirre GD, Patterson DF, Haskins ME, Henthorn PS
Genomics48(2)248-53(1998 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a novel genetic defect in the Brazilian Terrier breed causing a congenital skeletal dysplasia
Title: A novel GUSB mutation in Brazilian terriers with severe skeletal abnormalities defines the disease as mucopolysaccharidosis VII.

The following GUSB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUSB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe34164	NM_001003191.1 Latest version!	Canis lupus familiaris glucuronidase beta (GUSB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe07336	XM_005620897.3 Latest version!	Canis lupus familiaris glucuronidase beta (GUSB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe36795	XM_014114125.2 Latest version!	Canis lupus familiaris glucuronidase beta (GUSB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCe36796	XM_014114126.2 Latest version!	Canis lupus familiaris glucuronidase beta (GUSB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
OCe36795	XM_014114125.1	Canis lupus familiaris glucuronidase, beta (GUSB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCe36796	XM_014114126.1	Canis lupus familiaris glucuronidase, beta (GUSB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCe07336	XM_005620897.2	Canis lupus familiaris glucuronidase, beta (GUSB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUSB ( NM_001003191.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003191.1 Canis lupus familiaris glucuronidase beta (GUSB), mRNA.

Price & Availability↑

CloneID	OCe34164
Clone ID Related Accession (Same CDS sequence)	NM_001003191.1
Accession Version	NM_001003191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Canis lupus familiaris(dog)
Product	beta-glucuronidase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF019759.1. ##Evidence-Data-START## Transcript exon combination :: AF019759.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGTCTCGGG GGCCGGCGGG GGCCTGGGTA GCGCTCGGCC CGCTGCTTTG GACCTGCGGG 
CTGGCGCTGG AGGGCGGGAT GCTGTACCCC CGGGAGAGCC CGTCGCGGGA GCGCAAGGAC 
CTCGACGGCC TCTGGAGCTT CCGCGCCGAC TTCTCCGACG GCCGGCGCCA GGGCTTCGAG 
CAGCAGTGGT ACCGGGCGCC GCTGCGCGAG TCGGGCCCCA CCCTGGACAT GCCAGTTCCG 
TCCAGCTTCA ATGATGTGGG CCAGGATAGG CAGCTGCGCA GCTTTGTCGG CTGGGTGTGG 
TACGAGCGGG AGGCCACCCT GCCCCGGCGA TGGAGCCAGG ACCCGGGCAC GAGAGTGGTG 
CTGAGGATTG GCAGCGCCCA CTACTATGCC ATCGTGTGGG TGAATGGGGT CCATGTGGCA 
GAGCACGAGG GGGGTCACCT CCCCTTCGAA GCTGACATCA GCAAGTTGGT CCAGAGCGGG 
CCCCTGTCCT CCTGCCGTAT TACCCTTGCC ATCAACAACA CGCTCACCCC CCACACTCTG 
CCGCCAGGGA CCATCGTCTA CAAGACAGAC GCTTCCAAGT ACCCCAAGGG TTACTTCGTC 
CAGAACACAT ACTTTGACTT CTTCAACTAC GCGGGCCTGC ATCGCCCTGT GCTCCTCTAC 
ACCACACCTA CTACCTACAT CGACGACATC ACCGTCACCA CCGGCGTGGA CCAAGACACT 
GGGCTGGTGG ATTACCAGAT TTTTGTCCAG GGCAGTGAAC ACTTCCAGCT GGAAGTGTAT 
CTTCTGGATG AGGAAGGCAA GGTCGTGGCC CAGGGGACAG GGAGCCAGGG CCGGCTGCAG 
GTGCCCAATG TCCACCTCTG GTGGCCGTAC CTGATGCATG AGCACCCCGC CTACCTGTAC 
TCGTTGGAGG TGAGGCTGAC TGCGCAGATG GCCGCTGGGC CTGTGTCAGA CTTCTATACT 
CTCCCCGTGG GGATTCGCAC CGTGGCCGTC ACAGAGCGCC AGTTCCTCAT CAACGGGAAA 
CCTTTCTATT TCCATGGGGT CAACAAACAT GAGGATGCCG ATATCCGAGG GAAGGGCTTT 
GACTGGCCGC TGCTGGTGAA GGACTTCAAC CTGTTGCGCT GGCTGGGCGC CAATGCCTTC 
CGCACCAGCC ACTACCCCTA CGCGGAGGAG GTGATGCAGC TCTGCGACCG CTATGGGATC 
GTGGTCATCG ACGAGAGCCC TGGTGTGGGC ATCATGCTGG TCCAGAGCTA CAGCAATGTG 
TCCCTGCAGC ACCATCTGGA GGTGATGGGG GAGCTGGTGC GTCGGGATAA GAATCACCCA 
TCTGTAGTCA TGTGGTCTGT AGCCAATGAG CCCACTTCCT TCCTGAAGCC TGCTGCTTAC 
TACTTCAAGA CGCTGATTGC TCACACCAAG GCCTTGGACC CCTCCCGGCC CGTGACCTTT 
GTGACCAATT CCAACTATGA AGCAGACCTG GGGGCGCCGT ATGTGGACGT CATCTGTGTC 
AACAGTTACT ACTCTTGGTA TCACGACTAT GGGCACATGG AGGTGATTCA GCTGCAGCTG 
GCCACCGAGT TTGAGAACTG GTATAGGACC TACCAGAAAC CAATAATCCA GAGCGAGTAC 
GGGGCAGAGA CAATTGCAGG CTTCCACCAG GATCCACCTC TGATGTTCAG TGAGGAGTAC 
CAGAAAGGTC TGCTCGAGCA GTATCACTTG GTGCTGGATC AGAAACGCAA AGAATATGTG 
GTTGGAGAGC TCATCTGGAA TTTTGCTGAT TTTATGACTG ACCAGTCACC ACAGAGAGCA 
GTAGGGAACA GAAAGGGCAT CTTCACTCGC CAGAGACAAC CCAAAGCGGC GGCCTTCCTT 
TTGCGAGAGA GGTACTGGAA ACTTGCCAAT GAAACCGGGC ACCACCGGTC CGCGGCCAAG 
TCCCAGTGTT TGGAAAACAG CCCGTTCGCC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003191.1
CDS	63..2018
Translation	MSRGPAGAWVALGPLLWTCGLALEGGMLYPRESPSRERKDLDGLWSFRADFSDGRRQGFEQQWYRAPLRESGPTLDMPVPSSFNDVGQDRQLRSFVGWVWYEREATLPRRWSQDPGTRVVLRIGSAHYYAIVWVNGVHVAEHEGGHLPFEADISKLVQSGPLSSCRITLAINNTLTPHTLPPGTIVYKTDASKYPKGYFVQNTYFDFFNYAGLHRPVLLYTTPTTYIDDITVTTGVDQDTGLVDYQIFVQGSEHFQLEVYLLDEEGKVVAQGTGSQGRLQVPNVHLWWPYLMHEHPAYLYSLEVRLTAQMAAGPVSDFYTLPVGIRTVAVTERQFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEVMQLCDRYGIVVIDESPGVGIMLVQSYSNVSLQHHLEVMGELVRRDKNHPSVVMWSVANEPTSFLKPAAYYFKTLIAHTKALDPSRPVTFVTNSNYEADLGAPYVDVICVNSYYSWYHDYGHMEVIQLQLATEFENWYRTYQKPIIQSEYGAETIAGFHQDPPLMFSEEYQKGLLEQYHLVLDQKRKEYVVGELIWNFADFMTDQSPQRAVGNRKGIFTRQRQPKAAAFLLRERYWKLANETGHHRSAAKSQCLENSPFAL

Target ORF information:

RefSeq Version	NM_001003191.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucuronidase beta (GUSB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003191.1

ORF Insert Sequence:

ATGTCTCGGG GGCCGGCGGG GGCCTGGGTA GCGCTCGGCC CGCTGCTTTG GACCTGCGGG 
CTGGCGCTGG AGGGCGGGAT GCTGTACCCC CGGGAGAGCC CGTCGCGGGA GCGCAAGGAC 
CTCGACGGCC TCTGGAGCTT CCGCGCCGAC TTCTCCGACG GCCGGCGCCA GGGCTTCGAG 
CAGCAGTGGT ACCGGGCGCC GCTGCGCGAG TCGGGCCCCA CCCTGGACAT GCCAGTTCCG 
TCCAGCTTCA ATGATGTGGG CCAGGATAGG CAGCTGCGCA GCTTTGTCGG CTGGGTGTGG 
TACGAGCGGG AGGCCACCCT GCCCCGGCGA TGGAGCCAGG ACCCGGGCAC GAGAGTGGTG 
CTGAGGATTG GCAGCGCCCA CTACTATGCC ATCGTGTGGG TGAATGGGGT CCATGTGGCA 
GAGCACGAGG GGGGTCACCT CCCCTTCGAA GCTGACATCA GCAAGTTGGT CCAGAGCGGG 
CCCCTGTCCT CCTGCCGTAT TACCCTTGCC ATCAACAACA CGCTCACCCC CCACACTCTG 
CCGCCAGGGA CCATCGTCTA CAAGACAGAC GCTTCCAAGT ACCCCAAGGG TTACTTCGTC 
CAGAACACAT ACTTTGACTT CTTCAACTAC GCGGGCCTGC ATCGCCCTGT GCTCCTCTAC 
ACCACACCTA CTACCTACAT CGACGACATC ACCGTCACCA CCGGCGTGGA CCAAGACACT 
GGGCTGGTGG ATTACCAGAT TTTTGTCCAG GGCAGTGAAC ACTTCCAGCT GGAAGTGTAT 
CTTCTGGATG AGGAAGGCAA GGTCGTGGCC CAGGGGACAG GGAGCCAGGG CCGGCTGCAG 
GTGCCCAATG TCCACCTCTG GTGGCCGTAC CTGATGCATG AGCACCCCGC CTACCTGTAC 
TCGTTGGAGG TGAGGCTGAC TGCGCAGATG GCCGCTGGGC CTGTGTCAGA CTTCTATACT 
CTCCCCGTGG GGATTCGCAC CGTGGCCGTC ACAGAGCGCC AGTTCCTCAT CAACGGGAAA 
CCTTTCTATT TCCATGGGGT CAACAAACAT GAGGATGCCG ATATCCGAGG GAAGGGCTTT 
GACTGGCCGC TGCTGGTGAA GGACTTCAAC CTGTTGCGCT GGCTGGGCGC CAATGCCTTC 
CGCACCAGCC ACTACCCCTA CGCGGAGGAG GTGATGCAGC TCTGCGACCG CTATGGGATC 
GTGGTCATCG ACGAGAGCCC TGGTGTGGGC ATCATGCTGG TCCAGAGCTA CAGCAATGTG 
TCCCTGCAGC ACCATCTGGA GGTGATGGGG GAGCTGGTGC GTCGGGATAA GAATCACCCA 
TCTGTAGTCA TGTGGTCTGT AGCCAATGAG CCCACTTCCT TCCTGAAGCC TGCTGCTTAC 
TACTTCAAGA CGCTGATTGC TCACACCAAG GCCTTGGACC CCTCCCGGCC CGTGACCTTT 
GTGACCAATT CCAACTATGA AGCAGACCTG GGGGCGCCGT ATGTGGACGT CATCTGTGTC 
AACAGTTACT ACTCTTGGTA TCACGACTAT GGGCACATGG AGGTGATTCA GCTGCAGCTG 
GCCACCGAGT TTGAGAACTG GTATAGGACC TACCAGAAAC CAATAATCCA GAGCGAGTAC 
GGGGCAGAGA CAATTGCAGG CTTCCACCAG GATCCACCTC TGATGTTCAG TGAGGAGTAC 
CAGAAAGGTC TGCTCGAGCA GTATCACTTG GTGCTGGATC AGAAACGCAA AGAATATGTG 
GTTGGAGAGC TCATCTGGAA TTTTGCTGAT TTTATGACTG ACCAGTCACC ACAGAGAGCA 
GTAGGGAACA GAAAGGGCAT CTTCACTCGC CAGAGACAAC CCAAAGCGGC GGCCTTCCTT 
TTGCGAGAGA GGTACTGGAA ACTTGCCAAT GAAACCGGGC ACCACCGGTC CGCGGCCAAG 
TCCCAGTGTT TGGAAAACAG CCCGTTCGCC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUSB ( XM_005620897.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620954.1 Canis lupus familiaris glucuronidase, beta (GUSB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe07336
Clone ID Related Accession (Same CDS sequence)	XM_005620897.2 , XM_005620897.3
Accession Version	XM_005620897.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	beta-glucuronidase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726061.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTC CGTCCAGCTT CAATGATGTG GGCCAGGATA GGCAGCTGCG CAGCTTTGTC 
GGCTGGGTGT GGTACGAGCG GGAGGCCACC CTGCCCCGGC GATGGAGCCA GGACCCGGGC 
ACGAGAGTGG TGCTGAGGAT TGGCAGCGCC CACTACTATG CCATCGTGTG GGTGAATGGG 
GTCCATGTGG CAGAGCACGA GGGGGGTCAC CTCCCCTTCG AAGCTGACAT CAGCAAGTTG 
GTCCAGAGCG GGCCCCTGTC CTCCTGCCGT ATTACCCTTG CCATCAACAA CACGCTCACC 
CCCCACACTC TGCCGCCAGG GACCATCGTC TACAAGACAG ACGCTTCCAA GTACCCCAAG 
GGTTACTTCG TCCAGAACAC ATACTTTGAC TTCTTCAACT ACGCGGGCCT GCATCGCCCT 
GTGCTCCTCT ACACCACACC TACTACCTAC ATCGACGACA TCACCGTCAC CACCGGCGTG 
GACCAAGACA CTGGGCTGGT GGATTACCAG ATTTTTGTCC AGGGCAGTGA ACACTTCCAG 
CTGGAAGTGT ATCTTCTGGA TGAGGAAGGC AAGGTCGTGG CCCAGGGGAC AGGGAGCCAG 
GGCCGGCTGC AGGTGCCCAA TGTCCACCTC TGGTGGCCGT ACCTGATGCA TGAGCACCCC 
GCCTACCTGT ACTCGTTGGA GGTGAGGCTG ACTGCGCAGA TGGCCGCTGG GCCTGTGTCA 
GACTTCTATA CTCTCCCCGT GGGGATTCGC ACCGTGGCCG TCACAGAGCG CCAGTTCCTC 
ATCAACGGGA AACCTTTCTA TTTCCATGGG GTCAACAAAC ATGAGGATGC CGATATCCGA 
GGGAAGGGCT TTGACTGGCC GCTGCTGGTG AAGGACTTCA ACCTGTTGCG CTGGCTGGGC 
GCCAATGCCT TCCGCACCAG CCACTACCCC TACGCGGAGG AGGTGATGCA GCTCTGCGAC 
CGCTATGGGA TCGTGGTCAT CGACGAGAGC CCTGGTTTGG GCATCATGCT GGTCCAGAGC 
TACAGCAATG TGTCCCTGCA GCACCATCTG GAGGTGATGG GGGAGCTGGT GCGTCGGGAT 
AAGAATCACC CATCTGTAGT CATGTGGTCT GTAGCCAATG AGCCCACTTC CTTCCTGAAG 
CCTGCTGCTT ACTACTTCAA GACGCTGATT GCTCACACCA AGGCCTTGGA CCCCTCCCGG 
CCCGTGACCT TTGTGACCAA TTCCAACTAT GAAGCAGACC TGGGGGCACC GTATGTGGAC 
GTCATCTGTG TCAACAGTTA CTACTCTTGG TATCACGACT ATGGGCACAT GGAGGTGATT 
CAGCTGCAGC TGGCCACCGA GTTTGAGAAC TGGTATAGGA CCTACCAGAA ACCAATAATC 
CAGAGCGAGT ACGGGGCAGA GACAATTGCA GGCTTCCACC AGGATCCACC TCTGATGTTC 
AGTGAGGAGT ACCAGAAAGG TCTGCTCGAG CAGTATCACT TGGTGCTGGA TCAGAAACGC 
AAAGAATATG TGGTTGGAGA GCTCATCTGG AATTTTGCTG ATTTTATGAC TGACCAGTCA 
CCACAGAGAG CAGTAGGGAA CAGAAAGGGC ATCTTCACTC GCCAGAGACA ACCCAAAGCG 
GCGGCCTTCC TTTTGCGAGA GAGGTACTGG AAACTTGCCA ATGAAACCGG GCACCACCGG 
TCCGCGGCCA AGTCCCAGTG TTTGGAAAAC AGCCCGTTCG CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620954.1
CDS	196..1923
Translation	MPVPSSFNDVGQDRQLRSFVGWVWYEREATLPRRWSQDPGTRVVLRIGSAHYYAIVWVNGVHVAEHEGGHLPFEADISKLVQSGPLSSCRITLAINNTLTPHTLPPGTIVYKTDASKYPKGYFVQNTYFDFFNYAGLHRPVLLYTTPTTYIDDITVTTGVDQDTGLVDYQIFVQGSEHFQLEVYLLDEEGKVVAQGTGSQGRLQVPNVHLWWPYLMHEHPAYLYSLEVRLTAQMAAGPVSDFYTLPVGIRTVAVTERQFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEVMQLCDRYGIVVIDESPGLGIMLVQSYSNVSLQHHLEVMGELVRRDKNHPSVVMWSVANEPTSFLKPAAYYFKTLIAHTKALDPSRPVTFVTNSNYEADLGAPYVDVICVNSYYSWYHDYGHMEVIQLQLATEFENWYRTYQKPIIQSEYGAETIAGFHQDPPLMFSEEYQKGLLEQYHLVLDQKRKEYVVGELIWNFADFMTDQSPQRAVGNRKGIFTRQRQPKAAAFLLRERYWKLANETGHHRSAAKSQCLENSPFAL

Target ORF information:

RefSeq Version	XM_005620897.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris glucuronidase, beta (GUSB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620897.2

ORF Insert Sequence:

ATGCCAGTTC CGTCCAGCTT CAATGATGTG GGCCAGGATA GGCAGCTGCG CAGCTTTGTC 
GGCTGGGTGT GGTACGAGCG GGAGGCCACC CTGCCCCGGC GATGGAGCCA GGACCCGGGC 
ACGAGAGTGG TGCTGAGGAT TGGCAGCGCC CACTACTATG CCATCGTGTG GGTGAATGGG 
GTCCATGTGG CAGAGCACGA GGGGGGTCAC CTCCCCTTCG AAGCTGACAT CAGCAAGTTG 
GTCCAGAGCG GGCCCCTGTC CTCCTGCCGT ATTACCCTTG CCATCAACAA CACGCTCACC 
CCCCACACTC TGCCGCCAGG GACCATCGTC TACAAGACAG ACGCTTCCAA GTACCCCAAG 
GGTTACTTCG TCCAGAACAC ATACTTTGAC TTCTTCAACT ACGCGGGCCT GCATCGCCCT 
GTGCTCCTCT ACACCACACC TACTACCTAC ATCGACGACA TCACCGTCAC CACCGGCGTG 
GACCAAGACA CTGGGCTGGT GGATTACCAG ATTTTTGTCC AGGGCAGTGA ACACTTCCAG 
CTGGAAGTGT ATCTTCTGGA TGAGGAAGGC AAGGTCGTGG CCCAGGGGAC AGGGAGCCAG 
GGCCGGCTGC AGGTGCCCAA TGTCCACCTC TGGTGGCCGT ACCTGATGCA TGAGCACCCC 
GCCTACCTGT ACTCGTTGGA GGTGAGGCTG ACTGCGCAGA TGGCCGCTGG GCCTGTGTCA 
GACTTCTATA CTCTCCCCGT GGGGATTCGC ACCGTGGCCG TCACAGAGCG CCAGTTCCTC 
ATCAACGGGA AACCTTTCTA TTTCCATGGG GTCAACAAAC ATGAGGATGC CGATATCCGA 
GGGAAGGGCT TTGACTGGCC GCTGCTGGTG AAGGACTTCA ACCTGTTGCG CTGGCTGGGC 
GCCAATGCCT TCCGCACCAG CCACTACCCC TACGCGGAGG AGGTGATGCA GCTCTGCGAC 
CGCTATGGGA TCGTGGTCAT CGACGAGAGC CCTGGTTTGG GCATCATGCT GGTCCAGAGC 
TACAGCAATG TGTCCCTGCA GCACCATCTG GAGGTGATGG GGGAGCTGGT GCGTCGGGAT 
AAGAATCACC CATCTGTAGT CATGTGGTCT GTAGCCAATG AGCCCACTTC CTTCCTGAAG 
CCTGCTGCTT ACTACTTCAA GACGCTGATT GCTCACACCA AGGCCTTGGA CCCCTCCCGG 
CCCGTGACCT TTGTGACCAA TTCCAACTAT GAAGCAGACC TGGGGGCACC GTATGTGGAC 
GTCATCTGTG TCAACAGTTA CTACTCTTGG TATCACGACT ATGGGCACAT GGAGGTGATT 
CAGCTGCAGC TGGCCACCGA GTTTGAGAAC TGGTATAGGA CCTACCAGAA ACCAATAATC 
CAGAGCGAGT ACGGGGCAGA GACAATTGCA GGCTTCCACC AGGATCCACC TCTGATGTTC 
AGTGAGGAGT ACCAGAAAGG TCTGCTCGAG CAGTATCACT TGGTGCTGGA TCAGAAACGC 
AAAGAATATG TGGTTGGAGA GCTCATCTGG AATTTTGCTG ATTTTATGAC TGACCAGTCA 
CCACAGAGAG CAGTAGGGAA CAGAAAGGGC ATCTTCACTC GCCAGAGACA ACCCAAAGCG 
GCGGCCTTCC TTTTGCGAGA GAGGTACTGG AAACTTGCCA ATGAAACCGG GCACCACCGG 
TCCGCGGCCA AGTCCCAGTG TTTGGAAAAC AGCCCGTTCG CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUSB ( XM_005620897.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620954.1 Canis lupus familiaris glucuronidase beta (GUSB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe07336
Clone ID Related Accession (Same CDS sequence)	XM_005620897.2 , XM_005620897.3
Accession Version	XM_005620897.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	beta-glucuronidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620897.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTC CGTCCAGCTT CAATGATGTG GGCCAGGATA GGCAGCTGCG CAGCTTTGTC 
GGCTGGGTGT GGTACGAGCG GGAGGCCACC CTGCCCCGGC GATGGAGCCA GGACCCGGGC 
ACGAGAGTGG TGCTGAGGAT TGGCAGCGCC CACTACTATG CCATCGTGTG GGTGAATGGG 
GTCCATGTGG CAGAGCACGA GGGGGGTCAC CTCCCCTTCG AAGCTGACAT CAGCAAGTTG 
GTCCAGAGCG GGCCCCTGTC CTCCTGCCGT ATTACCCTTG CCATCAACAA CACGCTCACC 
CCCCACACTC TGCCGCCAGG GACCATCGTC TACAAGACAG ACGCTTCCAA GTACCCCAAG 
GGTTACTTCG TCCAGAACAC ATACTTTGAC TTCTTCAACT ACGCGGGCCT GCATCGCCCT 
GTGCTCCTCT ACACCACACC TACTACCTAC ATCGACGACA TCACCGTCAC CACCGGCGTG 
GACCAAGACA CTGGGCTGGT GGATTACCAG ATTTTTGTCC AGGGCAGTGA ACACTTCCAG 
CTGGAAGTGT ATCTTCTGGA TGAGGAAGGC AAGGTCGTGG CCCAGGGGAC AGGGAGCCAG 
GGCCGGCTGC AGGTGCCCAA TGTCCACCTC TGGTGGCCGT ACCTGATGCA TGAGCACCCC 
GCCTACCTGT ACTCGTTGGA GGTGAGGCTG ACTGCGCAGA TGGCCGCTGG GCCTGTGTCA 
GACTTCTATA CTCTCCCCGT GGGGATTCGC ACCGTGGCCG TCACAGAGCG CCAGTTCCTC 
ATCAACGGGA AACCTTTCTA TTTCCATGGG GTCAACAAAC ATGAGGATGC CGATATCCGA 
GGGAAGGGCT TTGACTGGCC GCTGCTGGTG AAGGACTTCA ACCTGTTGCG CTGGCTGGGC 
GCCAATGCCT TCCGCACCAG CCACTACCCC TACGCGGAGG AGGTGATGCA GCTCTGCGAC 
CGCTATGGGA TCGTGGTCAT CGACGAGAGC CCTGGTTTGG GCATCATGCT GGTCCAGAGC 
TACAGCAATG TGTCCCTGCA GCACCATCTG GAGGTGATGG GGGAGCTGGT GCGTCGGGAT 
AAGAATCACC CATCTGTAGT CATGTGGTCT GTAGCCAATG AGCCCACTTC CTTCCTGAAG 
CCTGCTGCTT ACTACTTCAA GACGCTGATT GCTCACACCA AGGCCTTGGA CCCCTCCCGG 
CCCGTGACCT TTGTGACCAA TTCCAACTAT GAAGCAGACC TGGGGGCACC GTATGTGGAC 
GTCATCTGTG TCAACAGTTA CTACTCTTGG TATCACGACT ATGGGCACAT GGAGGTGATT 
CAGCTGCAGC TGGCCACCGA GTTTGAGAAC TGGTATAGGA CCTACCAGAA ACCAATAATC 
CAGAGCGAGT ACGGGGCAGA GACAATTGCA GGCTTCCACC AGGATCCACC TCTGATGTTC 
AGTGAGGAGT ACCAGAAAGG TCTGCTCGAG CAGTATCACT TGGTGCTGGA TCAGAAACGC 
AAAGAATATG TGGTTGGAGA GCTCATCTGG AATTTTGCTG ATTTTATGAC TGACCAGTCA 
CCACAGAGAG CAGTAGGGAA CAGAAAGGGC ATCTTCACTC GCCAGAGACA ACCCAAAGCG 
GCGGCCTTCC TTTTGCGAGA GAGGTACTGG AAACTTGCCA ATGAAACCGG GCACCACCGG 
TCCGCGGCCA AGTCCCAGTG TTTGGAAAAC AGCCCGTTCG CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620954.1
CDS	243..1970
Translation	MPVPSSFNDVGQDRQLRSFVGWVWYEREATLPRRWSQDPGTRVVLRIGSAHYYAIVWVNGVHVAEHEGGHLPFEADISKLVQSGPLSSCRITLAINNTLTPHTLPPGTIVYKTDASKYPKGYFVQNTYFDFFNYAGLHRPVLLYTTPTTYIDDITVTTGVDQDTGLVDYQIFVQGSEHFQLEVYLLDEEGKVVAQGTGSQGRLQVPNVHLWWPYLMHEHPAYLYSLEVRLTAQMAAGPVSDFYTLPVGIRTVAVTERQFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEVMQLCDRYGIVVIDESPGLGIMLVQSYSNVSLQHHLEVMGELVRRDKNHPSVVMWSVANEPTSFLKPAAYYFKTLIAHTKALDPSRPVTFVTNSNYEADLGAPYVDVICVNSYYSWYHDYGHMEVIQLQLATEFENWYRTYQKPIIQSEYGAETIAGFHQDPPLMFSEEYQKGLLEQYHLVLDQKRKEYVVGELIWNFADFMTDQSPQRAVGNRKGIFTRQRQPKAAAFLLRERYWKLANETGHHRSAAKSQCLENSPFAL

Target ORF information:

RefSeq Version	XM_005620897.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucuronidase beta (GUSB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620897.3

ORF Insert Sequence:

ATGCCAGTTC CGTCCAGCTT CAATGATGTG GGCCAGGATA GGCAGCTGCG CAGCTTTGTC 
GGCTGGGTGT GGTACGAGCG GGAGGCCACC CTGCCCCGGC GATGGAGCCA GGACCCGGGC 
ACGAGAGTGG TGCTGAGGAT TGGCAGCGCC CACTACTATG CCATCGTGTG GGTGAATGGG 
GTCCATGTGG CAGAGCACGA GGGGGGTCAC CTCCCCTTCG AAGCTGACAT CAGCAAGTTG 
GTCCAGAGCG GGCCCCTGTC CTCCTGCCGT ATTACCCTTG CCATCAACAA CACGCTCACC 
CCCCACACTC TGCCGCCAGG GACCATCGTC TACAAGACAG ACGCTTCCAA GTACCCCAAG 
GGTTACTTCG TCCAGAACAC ATACTTTGAC TTCTTCAACT ACGCGGGCCT GCATCGCCCT 
GTGCTCCTCT ACACCACACC TACTACCTAC ATCGACGACA TCACCGTCAC CACCGGCGTG 
GACCAAGACA CTGGGCTGGT GGATTACCAG ATTTTTGTCC AGGGCAGTGA ACACTTCCAG 
CTGGAAGTGT ATCTTCTGGA TGAGGAAGGC AAGGTCGTGG CCCAGGGGAC AGGGAGCCAG 
GGCCGGCTGC AGGTGCCCAA TGTCCACCTC TGGTGGCCGT ACCTGATGCA TGAGCACCCC 
GCCTACCTGT ACTCGTTGGA GGTGAGGCTG ACTGCGCAGA TGGCCGCTGG GCCTGTGTCA 
GACTTCTATA CTCTCCCCGT GGGGATTCGC ACCGTGGCCG TCACAGAGCG CCAGTTCCTC 
ATCAACGGGA AACCTTTCTA TTTCCATGGG GTCAACAAAC ATGAGGATGC CGATATCCGA 
GGGAAGGGCT TTGACTGGCC GCTGCTGGTG AAGGACTTCA ACCTGTTGCG CTGGCTGGGC 
GCCAATGCCT TCCGCACCAG CCACTACCCC TACGCGGAGG AGGTGATGCA GCTCTGCGAC 
CGCTATGGGA TCGTGGTCAT CGACGAGAGC CCTGGTTTGG GCATCATGCT GGTCCAGAGC 
TACAGCAATG TGTCCCTGCA GCACCATCTG GAGGTGATGG GGGAGCTGGT GCGTCGGGAT 
AAGAATCACC CATCTGTAGT CATGTGGTCT GTAGCCAATG AGCCCACTTC CTTCCTGAAG 
CCTGCTGCTT ACTACTTCAA GACGCTGATT GCTCACACCA AGGCCTTGGA CCCCTCCCGG 
CCCGTGACCT TTGTGACCAA TTCCAACTAT GAAGCAGACC TGGGGGCACC GTATGTGGAC 
GTCATCTGTG TCAACAGTTA CTACTCTTGG TATCACGACT ATGGGCACAT GGAGGTGATT 
CAGCTGCAGC TGGCCACCGA GTTTGAGAAC TGGTATAGGA CCTACCAGAA ACCAATAATC 
CAGAGCGAGT ACGGGGCAGA GACAATTGCA GGCTTCCACC AGGATCCACC TCTGATGTTC 
AGTGAGGAGT ACCAGAAAGG TCTGCTCGAG CAGTATCACT TGGTGCTGGA TCAGAAACGC 
AAAGAATATG TGGTTGGAGA GCTCATCTGG AATTTTGCTG ATTTTATGAC TGACCAGTCA 
CCACAGAGAG CAGTAGGGAA CAGAAAGGGC ATCTTCACTC GCCAGAGACA ACCCAAAGCG 
GCGGCCTTCC TTTTGCGAGA GAGGTACTGG AAACTTGCCA ATGAAACCGG GCACCACCGG 
TCCGCGGCCA AGTCCCAGTG TTTGGAAAAC AGCCCGTTCG CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUSB ( XM_014114125.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969600.1 Canis lupus familiaris glucuronidase beta (GUSB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe36795
Clone ID Related Accession (Same CDS sequence)	XM_014114125.2 , XM_014114125.1
Accession Version	XM_014114125.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	beta-glucuronidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014114125.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATCGT GGTACCCCAA GGGTTACTTC GTCCAGAACA CATACTTTGA CTTCTTCAAC 
TACGCGGGCC TGCATCGCCC TGTGCTCCTC TACACCACAC CTACTACCTA CATCGACGAC 
ATCACCGTCA CCACCGGCGT GGACCAAGAC ACTGGGCTGG TGGATTACCA GATTTTTGTC 
CAGGGCAGTG AACACTTCCA GCTGGAAGTG TATCTTCTGG ATGAGGAAGG CAAGGTCGTG 
GCCCAGGGGA CAGGGAGCCA GGGCCGGCTG CAGGTGCCCA ATGTCCACCT CTGGTGGCCG 
TACCTGATGC ATGAGCACCC CGCCTACCTG TACTCGTTGG AGGTGAGGCT GACTGCGCAG 
ATGGCCGCTG GGCCTGTGTC AGACTTCTAT ACTCTCCCCG TGGGGATTCG CACCGTGGCC 
GTCACAGAGC GCCAGTTCCT CATCAACGGG AAACCTTTCT ATTTCCATGG GGTCAACAAA 
CATGAGGATG CCGATATCCG AGGGAAGGGC TTTGACTGGC CGCTGCTGGT GAAGGACTTC 
AACCTGTTGC GCTGGCTGGG CGCCAATGCC TTCCGCACCA GCCACTACCC CTACGCGGAG 
GAGGTGATGC AGCTCTGCGA CCGCTATGGG ATCGTGGTCA TCGACGAGAG CCCTGGTTTG 
GGCATCATGC TGGTCCAGAG CTACAGCAAT GTGTCCCTGC AGCACCATCT GGAGGTGATG 
GGGGAGCTGG TGCGTCGGGA TAAGAATCAC CCATCTGTAG TCATGTGGTC TGTAGCCAAT 
GAGCCCACTT CCTTCCTGAA GCCTGCTGCT TACTACTTCA AGACGCTGAT TGCTCACACC 
AAGGCCTTGG ACCCCTCCCG GCCCGTGACC TTTGTGACCA ATTCCAACTA TGAAGCAGAC 
CTGGGGGCAC CGTATGTGGA CGTCATCTGT GTCAACAGTT ACTACTCTTG GTATCACGAC 
TATGGGCACA TGGAGGTGAT TCAGCTGCAG CTGGCCACCG AGTTTGAGAA CTGGTATAGG 
ACCTACCAGA AACCAATAAT CCAGAGCGAG TACGGGGCAG AGACAATTGC AGGCTTCCAC 
CAGGATCCAC CTCTGATGTT CAGTGAGGAG TACCAGAAAG GTCTGCTCGA GCAGTATCAC 
TTGGTGCTGG ATCAGAAACG CAAAGAATAT GTGGTTGGAG AGCTCATCTG GAATTTTGCT 
GATTTTATGA CTGACCAGTC ACCACAGAGA GCAGTAGGGA ACAGAAAGGG CATCTTCACT 
CGCCAGAGAC AACCCAAAGC GGCGGCCTTC CTTTTGCGAG AGAGGTACTG GAAACTTGCC 
AATGAAACCG GGCACCACCG GTCCGCGGCC AAGTCCCAGT GTTTGGAAAA CAGCCCGTTC 
GCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969600.1
CDS	133..1521
Translation	MPSWYPKGYFVQNTYFDFFNYAGLHRPVLLYTTPTTYIDDITVTTGVDQDTGLVDYQIFVQGSEHFQLEVYLLDEEGKVVAQGTGSQGRLQVPNVHLWWPYLMHEHPAYLYSLEVRLTAQMAAGPVSDFYTLPVGIRTVAVTERQFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEVMQLCDRYGIVVIDESPGLGIMLVQSYSNVSLQHHLEVMGELVRRDKNHPSVVMWSVANEPTSFLKPAAYYFKTLIAHTKALDPSRPVTFVTNSNYEADLGAPYVDVICVNSYYSWYHDYGHMEVIQLQLATEFENWYRTYQKPIIQSEYGAETIAGFHQDPPLMFSEEYQKGLLEQYHLVLDQKRKEYVVGELIWNFADFMTDQSPQRAVGNRKGIFTRQRQPKAAAFLLRERYWKLANETGHHRSAAKSQCLENSPFAL

Target ORF information:

RefSeq Version	XM_014114125.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucuronidase beta (GUSB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014114125.2

ORF Insert Sequence:

ATGCCATCGT GGTACCCCAA GGGTTACTTC GTCCAGAACA CATACTTTGA CTTCTTCAAC 
TACGCGGGCC TGCATCGCCC TGTGCTCCTC TACACCACAC CTACTACCTA CATCGACGAC 
ATCACCGTCA CCACCGGCGT GGACCAAGAC ACTGGGCTGG TGGATTACCA GATTTTTGTC 
CAGGGCAGTG AACACTTCCA GCTGGAAGTG TATCTTCTGG ATGAGGAAGG CAAGGTCGTG 
GCCCAGGGGA CAGGGAGCCA GGGCCGGCTG CAGGTGCCCA ATGTCCACCT CTGGTGGCCG 
TACCTGATGC ATGAGCACCC CGCCTACCTG TACTCGTTGG AGGTGAGGCT GACTGCGCAG 
ATGGCCGCTG GGCCTGTGTC AGACTTCTAT ACTCTCCCCG TGGGGATTCG CACCGTGGCC 
GTCACAGAGC GCCAGTTCCT CATCAACGGG AAACCTTTCT ATTTCCATGG GGTCAACAAA 
CATGAGGATG CCGATATCCG AGGGAAGGGC TTTGACTGGC CGCTGCTGGT GAAGGACTTC 
AACCTGTTGC GCTGGCTGGG CGCCAATGCC TTCCGCACCA GCCACTACCC CTACGCGGAG 
GAGGTGATGC AGCTCTGCGA CCGCTATGGG ATCGTGGTCA TCGACGAGAG CCCTGGTTTG 
GGCATCATGC TGGTCCAGAG CTACAGCAAT GTGTCCCTGC AGCACCATCT GGAGGTGATG 
GGGGAGCTGG TGCGTCGGGA TAAGAATCAC CCATCTGTAG TCATGTGGTC TGTAGCCAAT 
GAGCCCACTT CCTTCCTGAA GCCTGCTGCT TACTACTTCA AGACGCTGAT TGCTCACACC 
AAGGCCTTGG ACCCCTCCCG GCCCGTGACC TTTGTGACCA ATTCCAACTA TGAAGCAGAC 
CTGGGGGCAC CGTATGTGGA CGTCATCTGT GTCAACAGTT ACTACTCTTG GTATCACGAC 
TATGGGCACA TGGAGGTGAT TCAGCTGCAG CTGGCCACCG AGTTTGAGAA CTGGTATAGG 
ACCTACCAGA AACCAATAAT CCAGAGCGAG TACGGGGCAG AGACAATTGC AGGCTTCCAC 
CAGGATCCAC CTCTGATGTT CAGTGAGGAG TACCAGAAAG GTCTGCTCGA GCAGTATCAC 
TTGGTGCTGG ATCAGAAACG CAAAGAATAT GTGGTTGGAG AGCTCATCTG GAATTTTGCT 
GATTTTATGA CTGACCAGTC ACCACAGAGA GCAGTAGGGA ACAGAAAGGG CATCTTCACT 
CGCCAGAGAC AACCCAAAGC GGCGGCCTTC CTTTTGCGAG AGAGGTACTG GAAACTTGCC 
AATGAAACCG GGCACCACCG GTCCGCGGCC AAGTCCCAGT GTTTGGAAAA CAGCCCGTTC 
GCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUSB ( XM_014114125.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969600.1 Canis lupus familiaris glucuronidase, beta (GUSB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe36795
Clone ID Related Accession (Same CDS sequence)	XM_014114125.2 , XM_014114125.1
Accession Version	XM_014114125.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	beta-glucuronidase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726061.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATCGT GGTACCCCAA GGGTTACTTC GTCCAGAACA CATACTTTGA CTTCTTCAAC 
TACGCGGGCC TGCATCGCCC TGTGCTCCTC TACACCACAC CTACTACCTA CATCGACGAC 
ATCACCGTCA CCACCGGCGT GGACCAAGAC ACTGGGCTGG TGGATTACCA GATTTTTGTC 
CAGGGCAGTG AACACTTCCA GCTGGAAGTG TATCTTCTGG ATGAGGAAGG CAAGGTCGTG 
GCCCAGGGGA CAGGGAGCCA GGGCCGGCTG CAGGTGCCCA ATGTCCACCT CTGGTGGCCG 
TACCTGATGC ATGAGCACCC CGCCTACCTG TACTCGTTGG AGGTGAGGCT GACTGCGCAG 
ATGGCCGCTG GGCCTGTGTC AGACTTCTAT ACTCTCCCCG TGGGGATTCG CACCGTGGCC 
GTCACAGAGC GCCAGTTCCT CATCAACGGG AAACCTTTCT ATTTCCATGG GGTCAACAAA 
CATGAGGATG CCGATATCCG AGGGAAGGGC TTTGACTGGC CGCTGCTGGT GAAGGACTTC 
AACCTGTTGC GCTGGCTGGG CGCCAATGCC TTCCGCACCA GCCACTACCC CTACGCGGAG 
GAGGTGATGC AGCTCTGCGA CCGCTATGGG ATCGTGGTCA TCGACGAGAG CCCTGGTTTG 
GGCATCATGC TGGTCCAGAG CTACAGCAAT GTGTCCCTGC AGCACCATCT GGAGGTGATG 
GGGGAGCTGG TGCGTCGGGA TAAGAATCAC CCATCTGTAG TCATGTGGTC TGTAGCCAAT 
GAGCCCACTT CCTTCCTGAA GCCTGCTGCT TACTACTTCA AGACGCTGAT TGCTCACACC 
AAGGCCTTGG ACCCCTCCCG GCCCGTGACC TTTGTGACCA ATTCCAACTA TGAAGCAGAC 
CTGGGGGCAC CGTATGTGGA CGTCATCTGT GTCAACAGTT ACTACTCTTG GTATCACGAC 
TATGGGCACA TGGAGGTGAT TCAGCTGCAG CTGGCCACCG AGTTTGAGAA CTGGTATAGG 
ACCTACCAGA AACCAATAAT CCAGAGCGAG TACGGGGCAG AGACAATTGC AGGCTTCCAC 
CAGGATCCAC CTCTGATGTT CAGTGAGGAG TACCAGAAAG GTCTGCTCGA GCAGTATCAC 
TTGGTGCTGG ATCAGAAACG CAAAGAATAT GTGGTTGGAG AGCTCATCTG GAATTTTGCT 
GATTTTATGA CTGACCAGTC ACCACAGAGA GCAGTAGGGA ACAGAAAGGG CATCTTCACT 
CGCCAGAGAC AACCCAAAGC GGCGGCCTTC CTTTTGCGAG AGAGGTACTG GAAACTTGCC 
AATGAAACCG GGCACCACCG GTCCGCGGCC AAGTCCCAGT GTTTGGAAAA CAGCCCGTTC 
GCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969600.1
CDS	83..1471
Translation	MPSWYPKGYFVQNTYFDFFNYAGLHRPVLLYTTPTTYIDDITVTTGVDQDTGLVDYQIFVQGSEHFQLEVYLLDEEGKVVAQGTGSQGRLQVPNVHLWWPYLMHEHPAYLYSLEVRLTAQMAAGPVSDFYTLPVGIRTVAVTERQFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEVMQLCDRYGIVVIDESPGLGIMLVQSYSNVSLQHHLEVMGELVRRDKNHPSVVMWSVANEPTSFLKPAAYYFKTLIAHTKALDPSRPVTFVTNSNYEADLGAPYVDVICVNSYYSWYHDYGHMEVIQLQLATEFENWYRTYQKPIIQSEYGAETIAGFHQDPPLMFSEEYQKGLLEQYHLVLDQKRKEYVVGELIWNFADFMTDQSPQRAVGNRKGIFTRQRQPKAAAFLLRERYWKLANETGHHRSAAKSQCLENSPFAL

Target ORF information:

RefSeq Version	XM_014114125.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris glucuronidase, beta (GUSB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014114125.1

ORF Insert Sequence:

ATGCCATCGT GGTACCCCAA GGGTTACTTC GTCCAGAACA CATACTTTGA CTTCTTCAAC 
TACGCGGGCC TGCATCGCCC TGTGCTCCTC TACACCACAC CTACTACCTA CATCGACGAC 
ATCACCGTCA CCACCGGCGT GGACCAAGAC ACTGGGCTGG TGGATTACCA GATTTTTGTC 
CAGGGCAGTG AACACTTCCA GCTGGAAGTG TATCTTCTGG ATGAGGAAGG CAAGGTCGTG 
GCCCAGGGGA CAGGGAGCCA GGGCCGGCTG CAGGTGCCCA ATGTCCACCT CTGGTGGCCG 
TACCTGATGC ATGAGCACCC CGCCTACCTG TACTCGTTGG AGGTGAGGCT GACTGCGCAG 
ATGGCCGCTG GGCCTGTGTC AGACTTCTAT ACTCTCCCCG TGGGGATTCG CACCGTGGCC 
GTCACAGAGC GCCAGTTCCT CATCAACGGG AAACCTTTCT ATTTCCATGG GGTCAACAAA 
CATGAGGATG CCGATATCCG AGGGAAGGGC TTTGACTGGC CGCTGCTGGT GAAGGACTTC 
AACCTGTTGC GCTGGCTGGG CGCCAATGCC TTCCGCACCA GCCACTACCC CTACGCGGAG 
GAGGTGATGC AGCTCTGCGA CCGCTATGGG ATCGTGGTCA TCGACGAGAG CCCTGGTTTG 
GGCATCATGC TGGTCCAGAG CTACAGCAAT GTGTCCCTGC AGCACCATCT GGAGGTGATG 
GGGGAGCTGG TGCGTCGGGA TAAGAATCAC CCATCTGTAG TCATGTGGTC TGTAGCCAAT 
GAGCCCACTT CCTTCCTGAA GCCTGCTGCT TACTACTTCA AGACGCTGAT TGCTCACACC 
AAGGCCTTGG ACCCCTCCCG GCCCGTGACC TTTGTGACCA ATTCCAACTA TGAAGCAGAC 
CTGGGGGCAC CGTATGTGGA CGTCATCTGT GTCAACAGTT ACTACTCTTG GTATCACGAC 
TATGGGCACA TGGAGGTGAT TCAGCTGCAG CTGGCCACCG AGTTTGAGAA CTGGTATAGG 
ACCTACCAGA AACCAATAAT CCAGAGCGAG TACGGGGCAG AGACAATTGC AGGCTTCCAC 
CAGGATCCAC CTCTGATGTT CAGTGAGGAG TACCAGAAAG GTCTGCTCGA GCAGTATCAC 
TTGGTGCTGG ATCAGAAACG CAAAGAATAT GTGGTTGGAG AGCTCATCTG GAATTTTGCT 
GATTTTATGA CTGACCAGTC ACCACAGAGA GCAGTAGGGA ACAGAAAGGG CATCTTCACT 
CGCCAGAGAC AACCCAAAGC GGCGGCCTTC CTTTTGCGAG AGAGGTACTG GAAACTTGCC 
AATGAAACCG GGCACCACCG GTCCGCGGCC AAGTCCCAGT GTTTGGAAAA CAGCCCGTTC 
GCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUSB ( XM_014114126.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969601.1 Canis lupus familiaris glucuronidase, beta (GUSB), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe36796
Clone ID Related Accession (Same CDS sequence)	XM_014114126.1 , XM_014114126.2
Accession Version	XM_014114126.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	beta-glucuronidase isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726061.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAG GACCCGGGCA CGAGAGTGGT GCTGAGGATT GGCAGCGCCC ACTACTATGC 
CATCGTGGGC TGGTGGATTA CCAGATTTTT GTCCAGGGCA GTGAACACTT CCAGCTGGAA 
GTGTATCTTC TGGATGAGGA AGGCAAGGTC GTGGCCCAGG GGACAGGGAG CCAGGGCCGG 
CTGCAGGTGC CCAATGTCCA CCTCTGGTGG CCGTACCTGA TGCATGAGCA CCCCGCCTAC 
CTGTACTCGT TGGAGGTGAG GCTGACTGCG CAGATGGCCG CTGGGCCTGT GTCAGACTTC 
TATACTCTCC CCGTGGGGAT TCGCACCGTG GCCGTCACAG AGCGCCAGTT CCTCATCAAC 
GGGAAACCTT TCTATTTCCA TGGGGTCAAC AAACATGAGG ATGCCGATAT CCGAGGGAAG 
GGCTTTGACT GGCCGCTGCT GGTGAAGGAC TTCAACCTGT TGCGCTGGCT GGGCGCCAAT 
GCCTTCCGCA CCAGCCACTA CCCCTACGCG GAGGAGGTGA TGCAGCTCTG CGACCGCTAT 
GGGATCGTGG TCATCGACGA GAGCCCTGGT TTGGGCATCA TGCTGGTCCA GAGCTACAGC 
AATGTGTCCC TGCAGCACCA TCTGGAGGTG ATGGGGGAGC TGGTGCGTCG GGATAAGAAT 
CACCCATCTG TAGTCATGTG GTCTGTAGCC AATGAGCCCA CTTCCTTCCT GAAGCCTGCT 
GCTTACTACT TCAAGACGCT GATTGCTCAC ACCAAGGCCT TGGACCCCTC CCGGCCCGTG 
ACCTTTGTGA CCAATTCCAA CTATGAAGCA GACCTGGGGG CACCGTATGT GGACGTCATC 
TGTGTCAACA GTTACTACTC TTGGTATCAC GACTATGGGC ACATGGAGGT GATTCAGCTG 
CAGCTGGCCA CCGAGTTTGA GAACTGGTAT AGGACCTACC AGAAACCAAT AATCCAGAGC 
GAGTACGGGG CAGAGACAAT TGCAGGCTTC CACCAGGATC CACCTCTGAT GTTCAGTGAG 
GAGTACCAGA AAGGTCTGCT CGAGCAGTAT CACTTGGTGC TGGATCAGAA ACGCAAAGAA 
TATGTGGTTG GAGAGCTCAT CTGGAATTTT GCTGATTTTA TGACTGACCA GTCACCACAG 
AGAGCAGTAG GGAACAGAAA GGGCATCTTC ACTCGCCAGA GACAACCCAA AGCGGCGGCC 
TTCCTTTTGC GAGAGAGGTA CTGGAAACTT GCCAATGAAA CCGGGCACCA CCGGTCCGCG 
GCCAAGTCCC AGTGTTTGGA AAACAGCCCG TTCGCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969601.1
CDS	1..1302
Translation	MEPGPGHESGAEDWQRPLLCHRGLVDYQIFVQGSEHFQLEVYLLDEEGKVVAQGTGSQGRLQVPNVHLWWPYLMHEHPAYLYSLEVRLTAQMAAGPVSDFYTLPVGIRTVAVTERQFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEVMQLCDRYGIVVIDESPGLGIMLVQSYSNVSLQHHLEVMGELVRRDKNHPSVVMWSVANEPTSFLKPAAYYFKTLIAHTKALDPSRPVTFVTNSNYEADLGAPYVDVICVNSYYSWYHDYGHMEVIQLQLATEFENWYRTYQKPIIQSEYGAETIAGFHQDPPLMFSEEYQKGLLEQYHLVLDQKRKEYVVGELIWNFADFMTDQSPQRAVGNRKGIFTRQRQPKAAAFLLRERYWKLANETGHHRSAAKSQCLENSPFAL

Target ORF information:

RefSeq Version	XM_014114126.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris glucuronidase, beta (GUSB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014114126.1

ORF Insert Sequence:

ATGGAGCCAG GACCCGGGCA CGAGAGTGGT GCTGAGGATT GGCAGCGCCC ACTACTATGC 
CATCGTGGGC TGGTGGATTA CCAGATTTTT GTCCAGGGCA GTGAACACTT CCAGCTGGAA 
GTGTATCTTC TGGATGAGGA AGGCAAGGTC GTGGCCCAGG GGACAGGGAG CCAGGGCCGG 
CTGCAGGTGC CCAATGTCCA CCTCTGGTGG CCGTACCTGA TGCATGAGCA CCCCGCCTAC 
CTGTACTCGT TGGAGGTGAG GCTGACTGCG CAGATGGCCG CTGGGCCTGT GTCAGACTTC 
TATACTCTCC CCGTGGGGAT TCGCACCGTG GCCGTCACAG AGCGCCAGTT CCTCATCAAC 
GGGAAACCTT TCTATTTCCA TGGGGTCAAC AAACATGAGG ATGCCGATAT CCGAGGGAAG 
GGCTTTGACT GGCCGCTGCT GGTGAAGGAC TTCAACCTGT TGCGCTGGCT GGGCGCCAAT 
GCCTTCCGCA CCAGCCACTA CCCCTACGCG GAGGAGGTGA TGCAGCTCTG CGACCGCTAT 
GGGATCGTGG TCATCGACGA GAGCCCTGGT TTGGGCATCA TGCTGGTCCA GAGCTACAGC 
AATGTGTCCC TGCAGCACCA TCTGGAGGTG ATGGGGGAGC TGGTGCGTCG GGATAAGAAT 
CACCCATCTG TAGTCATGTG GTCTGTAGCC AATGAGCCCA CTTCCTTCCT GAAGCCTGCT 
GCTTACTACT TCAAGACGCT GATTGCTCAC ACCAAGGCCT TGGACCCCTC CCGGCCCGTG 
ACCTTTGTGA CCAATTCCAA CTATGAAGCA GACCTGGGGG CACCGTATGT GGACGTCATC 
TGTGTCAACA GTTACTACTC TTGGTATCAC GACTATGGGC ACATGGAGGT GATTCAGCTG 
CAGCTGGCCA CCGAGTTTGA GAACTGGTAT AGGACCTACC AGAAACCAAT AATCCAGAGC 
GAGTACGGGG CAGAGACAAT TGCAGGCTTC CACCAGGATC CACCTCTGAT GTTCAGTGAG 
GAGTACCAGA AAGGTCTGCT CGAGCAGTAT CACTTGGTGC TGGATCAGAA ACGCAAAGAA 
TATGTGGTTG GAGAGCTCAT CTGGAATTTT GCTGATTTTA TGACTGACCA GTCACCACAG 
AGAGCAGTAG GGAACAGAAA GGGCATCTTC ACTCGCCAGA GACAACCCAA AGCGGCGGCC 
TTCCTTTTGC GAGAGAGGTA CTGGAAACTT GCCAATGAAA CCGGGCACCA CCGGTCCGCG 
GCCAAGTCCC AGTGTTTGGA AAACAGCCCG TTCGCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUSB ( XM_014114126.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969601.1 Canis lupus familiaris glucuronidase beta (GUSB), transcript variant X3, mRNA.

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CloneID	OCe36796
Clone ID Related Accession (Same CDS sequence)	XM_014114126.1 , XM_014114126.2
Accession Version	XM_014114126.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	beta-glucuronidase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014114126.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAG GACCCGGGCA CGAGAGTGGT GCTGAGGATT GGCAGCGCCC ACTACTATGC 
CATCGTGGGC TGGTGGATTA CCAGATTTTT GTCCAGGGCA GTGAACACTT CCAGCTGGAA 
GTGTATCTTC TGGATGAGGA AGGCAAGGTC GTGGCCCAGG GGACAGGGAG CCAGGGCCGG 
CTGCAGGTGC CCAATGTCCA CCTCTGGTGG CCGTACCTGA TGCATGAGCA CCCCGCCTAC 
CTGTACTCGT TGGAGGTGAG GCTGACTGCG CAGATGGCCG CTGGGCCTGT GTCAGACTTC 
TATACTCTCC CCGTGGGGAT TCGCACCGTG GCCGTCACAG AGCGCCAGTT CCTCATCAAC 
GGGAAACCTT TCTATTTCCA TGGGGTCAAC AAACATGAGG ATGCCGATAT CCGAGGGAAG 
GGCTTTGACT GGCCGCTGCT GGTGAAGGAC TTCAACCTGT TGCGCTGGCT GGGCGCCAAT 
GCCTTCCGCA CCAGCCACTA CCCCTACGCG GAGGAGGTGA TGCAGCTCTG CGACCGCTAT 
GGGATCGTGG TCATCGACGA GAGCCCTGGT TTGGGCATCA TGCTGGTCCA GAGCTACAGC 
AATGTGTCCC TGCAGCACCA TCTGGAGGTG ATGGGGGAGC TGGTGCGTCG GGATAAGAAT 
CACCCATCTG TAGTCATGTG GTCTGTAGCC AATGAGCCCA CTTCCTTCCT GAAGCCTGCT 
GCTTACTACT TCAAGACGCT GATTGCTCAC ACCAAGGCCT TGGACCCCTC CCGGCCCGTG 
ACCTTTGTGA CCAATTCCAA CTATGAAGCA GACCTGGGGG CACCGTATGT GGACGTCATC 
TGTGTCAACA GTTACTACTC TTGGTATCAC GACTATGGGC ACATGGAGGT GATTCAGCTG 
CAGCTGGCCA CCGAGTTTGA GAACTGGTAT AGGACCTACC AGAAACCAAT AATCCAGAGC 
GAGTACGGGG CAGAGACAAT TGCAGGCTTC CACCAGGATC CACCTCTGAT GTTCAGTGAG 
GAGTACCAGA AAGGTCTGCT CGAGCAGTAT CACTTGGTGC TGGATCAGAA ACGCAAAGAA 
TATGTGGTTG GAGAGCTCAT CTGGAATTTT GCTGATTTTA TGACTGACCA GTCACCACAG 
AGAGCAGTAG GGAACAGAAA GGGCATCTTC ACTCGCCAGA GACAACCCAA AGCGGCGGCC 
TTCCTTTTGC GAGAGAGGTA CTGGAAACTT GCCAATGAAA CCGGGCACCA CCGGTCCGCG 
GCCAAGTCCC AGTGTTTGGA AAACAGCCCG TTCGCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969601.1
CDS	1..1302
Translation	MEPGPGHESGAEDWQRPLLCHRGLVDYQIFVQGSEHFQLEVYLLDEEGKVVAQGTGSQGRLQVPNVHLWWPYLMHEHPAYLYSLEVRLTAQMAAGPVSDFYTLPVGIRTVAVTERQFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEVMQLCDRYGIVVIDESPGLGIMLVQSYSNVSLQHHLEVMGELVRRDKNHPSVVMWSVANEPTSFLKPAAYYFKTLIAHTKALDPSRPVTFVTNSNYEADLGAPYVDVICVNSYYSWYHDYGHMEVIQLQLATEFENWYRTYQKPIIQSEYGAETIAGFHQDPPLMFSEEYQKGLLEQYHLVLDQKRKEYVVGELIWNFADFMTDQSPQRAVGNRKGIFTRQRQPKAAAFLLRERYWKLANETGHHRSAAKSQCLENSPFAL

Target ORF information:

RefSeq Version	XM_014114126.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucuronidase beta (GUSB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014114126.2

ORF Insert Sequence:

ATGGAGCCAG GACCCGGGCA CGAGAGTGGT GCTGAGGATT GGCAGCGCCC ACTACTATGC 
CATCGTGGGC TGGTGGATTA CCAGATTTTT GTCCAGGGCA GTGAACACTT CCAGCTGGAA 
GTGTATCTTC TGGATGAGGA AGGCAAGGTC GTGGCCCAGG GGACAGGGAG CCAGGGCCGG 
CTGCAGGTGC CCAATGTCCA CCTCTGGTGG CCGTACCTGA TGCATGAGCA CCCCGCCTAC 
CTGTACTCGT TGGAGGTGAG GCTGACTGCG CAGATGGCCG CTGGGCCTGT GTCAGACTTC 
TATACTCTCC CCGTGGGGAT TCGCACCGTG GCCGTCACAG AGCGCCAGTT CCTCATCAAC 
GGGAAACCTT TCTATTTCCA TGGGGTCAAC AAACATGAGG ATGCCGATAT CCGAGGGAAG 
GGCTTTGACT GGCCGCTGCT GGTGAAGGAC TTCAACCTGT TGCGCTGGCT GGGCGCCAAT 
GCCTTCCGCA CCAGCCACTA CCCCTACGCG GAGGAGGTGA TGCAGCTCTG CGACCGCTAT 
GGGATCGTGG TCATCGACGA GAGCCCTGGT TTGGGCATCA TGCTGGTCCA GAGCTACAGC 
AATGTGTCCC TGCAGCACCA TCTGGAGGTG ATGGGGGAGC TGGTGCGTCG GGATAAGAAT 
CACCCATCTG TAGTCATGTG GTCTGTAGCC AATGAGCCCA CTTCCTTCCT GAAGCCTGCT 
GCTTACTACT TCAAGACGCT GATTGCTCAC ACCAAGGCCT TGGACCCCTC CCGGCCCGTG 
ACCTTTGTGA CCAATTCCAA CTATGAAGCA GACCTGGGGG CACCGTATGT GGACGTCATC 
TGTGTCAACA GTTACTACTC TTGGTATCAC GACTATGGGC ACATGGAGGT GATTCAGCTG 
CAGCTGGCCA CCGAGTTTGA GAACTGGTAT AGGACCTACC AGAAACCAAT AATCCAGAGC 
GAGTACGGGG CAGAGACAAT TGCAGGCTTC CACCAGGATC CACCTCTGAT GTTCAGTGAG 
GAGTACCAGA AAGGTCTGCT CGAGCAGTAT CACTTGGTGC TGGATCAGAA ACGCAAAGAA 
TATGTGGTTG GAGAGCTCAT CTGGAATTTT GCTGATTTTA TGACTGACCA GTCACCACAG 
AGAGCAGTAG GGAACAGAAA GGGCATCTTC ACTCGCCAGA GACAACCCAA AGCGGCGGCC 
TTCCTTTTGC GAGAGAGGTA CTGGAAACTT GCCAATGAAA CCGGGCACCA CCGGTCCGCG 
GCCAAGTCCC AGTGTTTGGA AAACAGCCCG TTCGCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel GUSB mutation in Brazilian terriers with severe skeletal abnormalities defines the disease as mucopolysaccharidosis VII.
PloS one7(7)e40281(2012)
Hyt?nen MK,Arumilli M,Lappalainen AK,Kallio H,Snellman M,Sainio K,Lohi H

Construction and characterization of an eightfold redundant dog genomic bacterial artificial chromosome library.
Genomics58(1)9-17(1999 May)
Li R,Mignot E,Faraco J,Kadotani H,Cantanese J,Zhao B,Lin X,Hinton L,Ostrander EA,Patterson DF,de Jong PJ

Cloning of the canine beta-glucuronidase cDNA, mutation identification in canine MPS VII, and retroviral vector-mediated correction of MPS VII cells.
Genomics48(2)248-53(1998 Mar)
Ray J,Bouvet A,DeSanto C,Fyfe JC,Xu D,Wolfe JH,Aguirre GD,Patterson DF,Haskins ME,Henthorn PS

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GUSB cDNA ORF clone, Canis lupus familiaris(dog)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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