SPTAN1 cDNA ORF clone, Canis lupus familiaris(Dog)

The following SPTAN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTAN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe38674 XM_014117013.2
Latest version!
Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCe38675 XM_014117014.2
Latest version!
Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCe12754 XM_014117012.2
Latest version!
Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe12755 XM_005625282.3
Latest version!
Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe12756 XM_537823.6
Latest version!
Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe12757 XM_005625283.3
Latest version!
Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe12758 XM_005625284.3
Latest version!
Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe12759 XM_005625285.3
Latest version!
Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe12760 XM_005625286.3
Latest version!
Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe12761 XM_005625287.3
Latest version!
Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
OCe12757 XM_005625283.2 Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe12759 XM_005625285.2 Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe38674 XM_014117013.1 Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCe12756 XM_537823.5 Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe12760 XM_005625286.2 Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe12754 XM_014117012.1 Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe12755 XM_005625282.2 Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe12758 XM_005625284.2 Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe38675 XM_014117014.1 Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCe12761 XM_005625287.2 Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe38674
    Clone ID Related Accession (Same CDS sequence) XM_014117013.2 , XM_014117013.1
    Accession Version XM_014117013.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGGAGCT GAACGAGCGT TGGCGGTCCC TGCAGCAGCT GGCCGAGGAG
    CGGAGCCAGC TCTTGGGCAG CGCCCATGAA GTACAGAGGT TCCACAGAGA TGCCGATGAA
    ACCAAAGAGT GGATTGAGGA GAAGAATCAG GCCCTAAACA CAGACAACTA TGGCCACGAT
    CTGGCCAGCG TACAAGCTCT GCAGCGCAAG CACGAGGGCT TTGAGAGGGA CCTCGCGGCT
    CTTGGTGACA AGGTCAACTC CCTGGGGGAA ACAGCCGAGC GCCTGATCCA GTCCCACCCG
    GAGTCGGCCG AGGACCTGCA GGAAAAGTGC ACGGAGTTGG ACCAGGCGTG GAGCAGCCTG
    GGGAAGCGCG CAGACCAGCG CAAGGCCAAG CTGGGTGATT CCCACGACCT GCAACGCTTC
    CTTAGTGATT TCCGGGACCT CATGTCTTGG ATCAATGGAA TACGAGGGTT GGTGTCCTCA
    GACGAGTTAG CCAAGGATGT CACCGGAGCA GAAGCTTTGC TGGAACGGCA CCAGGAGCAC
    CGGACAGAAA TCGATGCCAG GGCTGGCACT TTCCAGGCCT TTGAGCAGTT TGGGCAGCAG
    CTGCTGGCTC ATGGGCACTA CGCCAGCCCA GAGGTCAAAG AGAAACTAGA TATTCTAGAT
    CGGGAGCGTG CGGGTCTGGA GAAGGCCTGG GCCCAGCGTA GGATGATGTT GGATCAGTGC
    CTTGAACTGC AGCTGTTTCA TCGGGACTGT GAACAAGCTG AGAACTGGAT GGCCGCCCGG
    GAGGCCTTTC TGAATACTGA GGACAAAGGA GACTCACTGG ACAGCGTGGA GGCTCTGATC
    AAAAAACACG AGGATTTTGA CAAAGCGATC AATGTCCAGG AGGAGAAGAT TGCCGCCTTG
    CAGTCCTTTG CTGACCAACT TATTGCTGCC GGCCACTATG CCAAGGGTGA CATCTGCAGT
    CGGCGCAATG AGGTTTTGGA CAGGTGGCGA CGCCTAAAAG CTCAGATGAT TGAGAAGAGA
    TCGAAGCTGG GAGAATCACA AACCCTCCAG CAGTTCAGCC GGGACGTGGA TGAGATCGAG
    GCTTGGATCA GTGAGAAATT ACAGACTGCG AGTGATGAGT CGTACAAAGA CCCCACCAAC
    ATCCAGCTTT CCAAGCTGCT GAGCAAACAC CAGAAGCACC AGGCCTTTGA GGCGGAACTG
    CATGCCAATG CTGACCGGAT CCGCGGGGTC ATCGACACGG GCAACTCCCT CATCGAGCGT
    GGGGCCTGTG CTGGCAGTGA GGATGCTGTC AAGGCCCGGC TGGCTGCCTT AGCTGACCAG
    TGGCAGTTCC TGGTGCAAAA GTCAGCCGAA AAGAGCCAGA AACTAAAAGA AGCCAACAAG
    CAGCAGAACT TCAACACTGG AATCAAGGAC TTTGACTTCT GGCTTTCTGA GGTGGAGGCC
    CTTCTGGCAT CTGAGGACTA CGGCAAAGAC CTGGCTTCTG TGAACAACTT GCTGAAGAAA
    CATCAACTGC TAGAGGCGGA CATATCTGCC CACGAGGATC GCCTGAAGGA CCTGAATAGC
    CAAGCAGACA GCCTGATGAC CAGCAGTGCC TTCGACACCT CCCAAGTGAA GGATAAGCGG
    GACACCATCA ATGGTCGTTT CCAGAAGATC AAGAACATGG CGGCCTCCCG GCGAGCCAGG
    CTGAATGAAT CCCATCGCCT ACACCAGTTC TTCCGGGACA TGGATGATGA GGAGTCCTGG
    ATCAAGGAGA AAAAGTTGCT GGTGAGCTCA GAGGACTATG GCCGAGACCT TACAGGAGTT
    CAGAACCTGA GGAAGAAACA CAAGCGGCTG GAGGCGGAAC TGGCTGCCCA TGAGCCAGCG
    ATCCAGGGTG TCCTGGACAC TGGGAAGAAG CTGTCTGATG ACAATACCAT TGGCAAGGAG
    GAGATTCAGC AGCGTCTGGC CCAGTTCGTG GAGCACTGGA AGGAGCTGAA GCAGCTGGCG
    GCAGCTCGGG GTCAGCGGCT AGAGGAGTCC TTGGAGTATC AGCAGTTTGT AGCCAATGTG
    GAAGAAGAAG AAGCCTGGAT TAATGAGAAG ATGACCCTGG TAGCCAGTGA GGATTATGGA
    GACACGCTTG CCGCTATCCA GGGCTTACTG AAGAAACATG AAGCTTTTGA GACAGACTTC
    ACTGTGCACA AGGATCGTGT GAACGACGTC TGCAGTAATG GACAAGACCT CATCAAGAAG
    AACAATCACC ACGAGGAGAA CATCTCTTCA AAGATGAAGG GCTTGAATGG CAAAGTGTCA
    GACTTGGAGA AAGCTGCTGC CCAGAGGAAG GCCAAGCTTG AGGAGAACTC GGCCTTCCTT
    CAGTTCAACT GGAAGGCTGA TGTAGTGGAA TCCTGGATCG GTGAGAAGGA AAACAGCCTG
    AAGACAGACG ATTATGGCCG AGACCTGTCC TCCGTGCAGA CTCTCCTCAC CAAACAGGAA
    ACTTTTGACG CTGGTCTACA GGCCTTCCAG CAGGAGGGCA TTGCCAACAT CACTGCCCTC
    AAAGATCAGC TGCTGGCCGC CAAGCACATT CAGTCAAAGG CCATTGAAGC CCGGCACGCC
    TCTCTCATGA AGAGATGGAG CCAGCTTCTG GCCAACTCGG CCACCCGCAA GAAGAAGCTG
    CTGGAGGCAC AGAGTCACTT CCGCAAGGTG GAAGACCTCT TCCTGACCTT CGCCAAAAAG
    GCATCGGCCT TCAACAGCTG GTTTGAAAAC GCCGAAGAGG ATCTGACTGA TCCTGTGCGT
    TGCAACTCCC TGGAGGAGAT CAGAGCTCTG CGTGAGGCCC ACGACGCCTT CCGCTCATCC
    CTCAGCTCTG CCCAGGCCGA CTTCAGCCAG CTGGCTGAGC TGGACCGCCA GATCAAGAGC
    TTCCGTGTGG CCTCTAACCC CTACACCTGG TTCACCATGG AGGCCCTGGA GGAGACGTGG
    AGGAACCTGC AGAAGATCAT CAAGGAGCGG GAGCTGGAGC TGCAAAAGGA GCAGCGGCGA
    CAGGAGGAGA ATGACAAGCT ACGCCAGGAG TTCGCCCAGC ACGCTAACGC CTTCCACCAG
    TGGATACAGG AAACCAGAAC ATATCTCCTC GATGGCATAG CGTATCGTCG GGTCATTCGT
    GTCTATCAGT ATGAAGTTGG GGATGATCTG TCTGGAAGGT CCTGCATGGT GGAAGAATCC
    GGGACCCTGG AATCCCAGCT TGAGGCTACC AAACGCAAGC ACCAGGAGAT TCGAGCCATG
    AGAAGTCAGC TCAAGAAGAT TGAGGACCTG GGGGCTGCCA TGGAGGAGGC CCTGATCCTG
    GATAACAAGT ATACAGAGCA CAGCACCGTG GGCCTGGCCC AACAGTGGGA CCAGCTCGAC
    CAGCTGGGCA TGCGCATGCA GCACAACCTG GAGCAGCAGA TCCAGGCCAG GAACACAACA
    GGAGTGACGG AGGAGGCCCT CAAAGAATTC AGCATGATGT TCAAACACTT CGACAAGGAC
    AAGTCAGGCC GGCTGAACCA CCAGGAGTTC AAGTCCTGCC TGCGCTCCCT GGGTTATGAC
    CTGCCCATGG TGGAGGAAGG GGAGCCTGAC CCCGAGTTCG AGGCAATCCT AGACACCGTG
    GATCCAAACA GGGATGGCCA CGTCTCCCTG CAAGAGTACA TGGCCTTCAT GATCAGCCGT
    GAAACGGAGA ACGTTAAATC CAGTGAGGAG ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC
    GAGGGAAAGC CCTACGTGAC CAAGGAGGAG CTGTATCAGA ACCTGACTCG AGAACAAGCC
    GACTACTGCG TCTCCCACAT GAAGCCCTAC GTGGATGGCA AGGGCCGCGA ACTCCCCACT
    GCCTTCGACT ACGTGGAGTT CACCCGCTCG CTCTTTGTGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972488.1
    CDS137..7621
    Translation

    Target ORF information:

    RefSeq Version XM_014117013.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117013.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGGAGCT GAACGAGCGT TGGCGGTCCC TGCAGCAGCT GGCCGAGGAG
    CGGAGCCAGC TCTTGGGCAG CGCCCATGAA GTACAGAGGT TCCACAGAGA TGCCGATGAA
    ACCAAAGAGT GGATTGAGGA GAAGAATCAG GCCCTAAACA CAGACAACTA TGGCCACGAT
    CTGGCCAGCG TACAAGCTCT GCAGCGCAAG CACGAGGGCT TTGAGAGGGA CCTCGCGGCT
    CTTGGTGACA AGGTCAACTC CCTGGGGGAA ACAGCCGAGC GCCTGATCCA GTCCCACCCG
    GAGTCGGCCG AGGACCTGCA GGAAAAGTGC ACGGAGTTGG ACCAGGCGTG GAGCAGCCTG
    GGGAAGCGCG CAGACCAGCG CAAGGCCAAG CTGGGTGATT CCCACGACCT GCAACGCTTC
    CTTAGTGATT TCCGGGACCT CATGTCTTGG ATCAATGGAA TACGAGGGTT GGTGTCCTCA
    GACGAGTTAG CCAAGGATGT CACCGGAGCA GAAGCTTTGC TGGAACGGCA CCAGGAGCAC
    CGGACAGAAA TCGATGCCAG GGCTGGCACT TTCCAGGCCT TTGAGCAGTT TGGGCAGCAG
    CTGCTGGCTC ATGGGCACTA CGCCAGCCCA GAGGTCAAAG AGAAACTAGA TATTCTAGAT
    CGGGAGCGTG CGGGTCTGGA GAAGGCCTGG GCCCAGCGTA GGATGATGTT GGATCAGTGC
    CTTGAACTGC AGCTGTTTCA TCGGGACTGT GAACAAGCTG AGAACTGGAT GGCCGCCCGG
    GAGGCCTTTC TGAATACTGA GGACAAAGGA GACTCACTGG ACAGCGTGGA GGCTCTGATC
    AAAAAACACG AGGATTTTGA CAAAGCGATC AATGTCCAGG AGGAGAAGAT TGCCGCCTTG
    CAGTCCTTTG CTGACCAACT TATTGCTGCC GGCCACTATG CCAAGGGTGA CATCTGCAGT
    CGGCGCAATG AGGTTTTGGA CAGGTGGCGA CGCCTAAAAG CTCAGATGAT TGAGAAGAGA
    TCGAAGCTGG GAGAATCACA AACCCTCCAG CAGTTCAGCC GGGACGTGGA TGAGATCGAG
    GCTTGGATCA GTGAGAAATT ACAGACTGCG AGTGATGAGT CGTACAAAGA CCCCACCAAC
    ATCCAGCTTT CCAAGCTGCT GAGCAAACAC CAGAAGCACC AGGCCTTTGA GGCGGAACTG
    CATGCCAATG CTGACCGGAT CCGCGGGGTC ATCGACACGG GCAACTCCCT CATCGAGCGT
    GGGGCCTGTG CTGGCAGTGA GGATGCTGTC AAGGCCCGGC TGGCTGCCTT AGCTGACCAG
    TGGCAGTTCC TGGTGCAAAA GTCAGCCGAA AAGAGCCAGA AACTAAAAGA AGCCAACAAG
    CAGCAGAACT TCAACACTGG AATCAAGGAC TTTGACTTCT GGCTTTCTGA GGTGGAGGCC
    CTTCTGGCAT CTGAGGACTA CGGCAAAGAC CTGGCTTCTG TGAACAACTT GCTGAAGAAA
    CATCAACTGC TAGAGGCGGA CATATCTGCC CACGAGGATC GCCTGAAGGA CCTGAATAGC
    CAAGCAGACA GCCTGATGAC CAGCAGTGCC TTCGACACCT CCCAAGTGAA GGATAAGCGG
    GACACCATCA ATGGTCGTTT CCAGAAGATC AAGAACATGG CGGCCTCCCG GCGAGCCAGG
    CTGAATGAAT CCCATCGCCT ACACCAGTTC TTCCGGGACA TGGATGATGA GGAGTCCTGG
    ATCAAGGAGA AAAAGTTGCT GGTGAGCTCA GAGGACTATG GCCGAGACCT TACAGGAGTT
    CAGAACCTGA GGAAGAAACA CAAGCGGCTG GAGGCGGAAC TGGCTGCCCA TGAGCCAGCG
    ATCCAGGGTG TCCTGGACAC TGGGAAGAAG CTGTCTGATG ACAATACCAT TGGCAAGGAG
    GAGATTCAGC AGCGTCTGGC CCAGTTCGTG GAGCACTGGA AGGAGCTGAA GCAGCTGGCG
    GCAGCTCGGG GTCAGCGGCT AGAGGAGTCC TTGGAGTATC AGCAGTTTGT AGCCAATGTG
    GAAGAAGAAG AAGCCTGGAT TAATGAGAAG ATGACCCTGG TAGCCAGTGA GGATTATGGA
    GACACGCTTG CCGCTATCCA GGGCTTACTG AAGAAACATG AAGCTTTTGA GACAGACTTC
    ACTGTGCACA AGGATCGTGT GAACGACGTC TGCAGTAATG GACAAGACCT CATCAAGAAG
    AACAATCACC ACGAGGAGAA CATCTCTTCA AAGATGAAGG GCTTGAATGG CAAAGTGTCA
    GACTTGGAGA AAGCTGCTGC CCAGAGGAAG GCCAAGCTTG AGGAGAACTC GGCCTTCCTT
    CAGTTCAACT GGAAGGCTGA TGTAGTGGAA TCCTGGATCG GTGAGAAGGA AAACAGCCTG
    AAGACAGACG ATTATGGCCG AGACCTGTCC TCCGTGCAGA CTCTCCTCAC CAAACAGGAA
    ACTTTTGACG CTGGTCTACA GGCCTTCCAG CAGGAGGGCA TTGCCAACAT CACTGCCCTC
    AAAGATCAGC TGCTGGCCGC CAAGCACATT CAGTCAAAGG CCATTGAAGC CCGGCACGCC
    TCTCTCATGA AGAGATGGAG CCAGCTTCTG GCCAACTCGG CCACCCGCAA GAAGAAGCTG
    CTGGAGGCAC AGAGTCACTT CCGCAAGGTG GAAGACCTCT TCCTGACCTT CGCCAAAAAG
    GCATCGGCCT TCAACAGCTG GTTTGAAAAC GCCGAAGAGG ATCTGACTGA TCCTGTGCGT
    TGCAACTCCC TGGAGGAGAT CAGAGCTCTG CGTGAGGCCC ACGACGCCTT CCGCTCATCC
    CTCAGCTCTG CCCAGGCCGA CTTCAGCCAG CTGGCTGAGC TGGACCGCCA GATCAAGAGC
    TTCCGTGTGG CCTCTAACCC CTACACCTGG TTCACCATGG AGGCCCTGGA GGAGACGTGG
    AGGAACCTGC AGAAGATCAT CAAGGAGCGG GAGCTGGAGC TGCAAAAGGA GCAGCGGCGA
    CAGGAGGAGA ATGACAAGCT ACGCCAGGAG TTCGCCCAGC ACGCTAACGC CTTCCACCAG
    TGGATACAGG AAACCAGAAC ATATCTCCTC GATGGCATAG CGTATCGTCG GGTCATTCGT
    GTCTATCAGT ATGAAGTTGG GGATGATCTG TCTGGAAGGT CCTGCATGGT GGAAGAATCC
    GGGACCCTGG AATCCCAGCT TGAGGCTACC AAACGCAAGC ACCAGGAGAT TCGAGCCATG
    AGAAGTCAGC TCAAGAAGAT TGAGGACCTG GGGGCTGCCA TGGAGGAGGC CCTGATCCTG
    GATAACAAGT ATACAGAGCA CAGCACCGTG GGCCTGGCCC AACAGTGGGA CCAGCTCGAC
    CAGCTGGGCA TGCGCATGCA GCACAACCTG GAGCAGCAGA TCCAGGCCAG GAACACAACA
    GGAGTGACGG AGGAGGCCCT CAAAGAATTC AGCATGATGT TCAAACACTT CGACAAGGAC
    AAGTCAGGCC GGCTGAACCA CCAGGAGTTC AAGTCCTGCC TGCGCTCCCT GGGTTATGAC
    CTGCCCATGG TGGAGGAAGG GGAGCCTGAC CCCGAGTTCG AGGCAATCCT AGACACCGTG
    GATCCAAACA GGGATGGCCA CGTCTCCCTG CAAGAGTACA TGGCCTTCAT GATCAGCCGT
    GAAACGGAGA ACGTTAAATC CAGTGAGGAG ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC
    GAGGGAAAGC CCTACGTGAC CAAGGAGGAG CTGTATCAGA ACCTGACTCG AGAACAAGCC
    GACTACTGCG TCTCCCACAT GAAGCCCTAC GTGGATGGCA AGGGCCGCGA ACTCCCCACT
    GCCTTCGACT ACGTGGAGTT CACCCGCTCG CTCTTTGTGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38674
    Clone ID Related Accession (Same CDS sequence) XM_014117013.2 , XM_014117013.1
    Accession Version XM_014117013.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    541
    601
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    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
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    2281
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    2641
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    4141
    4201
    4261
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    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
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    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
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    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGGAGCT GAACGAGCGT TGGCGGTCCC TGCAGCAGCT GGCCGAGGAG
    CGGAGCCAGC TCTTGGGCAG CGCCCATGAA GTACAGAGGT TCCACAGAGA TGCCGATGAA
    ACCAAAGAGT GGATTGAGGA GAAGAATCAG GCCCTAAACA CAGACAACTA TGGCCACGAT
    CTGGCCAGCG TACAAGCTCT GCAGCGCAAG CACGAGGGCT TTGAGAGGGA CCTCGCGGCT
    CTTGGTGACA AGGTCAACTC CCTGGGGGAA ACAGCCGAGC GCCTGATCCA GTCCCACCCG
    GAGTCGGCCG AGGACCTGCA GGAAAAGTGC ACGGAGTTGG ACCAGGCGTG GAGCAGCCTG
    GGGAAGCGCG CAGACCAGCG CAAGGCCAAG CTGGGTGATT CCCACGACCT GCAACGCTTC
    CTTAGTGATT TCCGGGACCT CATGTCTTGG ATCAATGGAA TACGAGGGTT GGTGTCCTCA
    GACGAGTTAG CCAAGGATGT CACCGGAGCA GAAGCTTTGC TGGAACGGCA CCAGGAGCAC
    CGGACAGAAA TCGATGCCAG GGCTGGCACT TTCCAGGCCT TTGAGCAGTT TGGGCAGCAG
    CTGCTGGCTC ATGGGCACTA CGCCAGCCCA GAGGTCAAAG AGAAACTAGA TATTCTAGAT
    CGGGAGCGTG CGGGTCTGGA GAAGGCCTGG GCCCAGCGTA GGATGATGTT GGATCAGTGC
    CTTGAACTGC AGCTGTTTCA TCGGGACTGT GAACAAGCTG AGAACTGGAT GGCCGCCCGG
    GAGGCCTTTC TGAATACTGA GGACAAAGGA GACTCACTGG ACAGCGTGGA GGCTCTGATC
    AAAAAACACG AGGATTTTGA CAAAGCGATC AATGTCCAGG AGGAGAAGAT TGCCGCCTTG
    CAGTCCTTTG CTGACCAACT TATTGCTGCC GGCCACTATG CCAAGGGTGA CATCTGCAGT
    CGGCGCAATG AGGTTTTGGA CAGGTGGCGA CGCCTAAAAG CTCAGATGAT TGAGAAGAGA
    TCGAAGCTGG GAGAATCACA AACCCTCCAG CAGTTCAGCC GGGACGTGGA TGAGATCGAG
    GCTTGGATCA GTGAGAAATT ACAGACTGCG AGTGATGAGT CGTACAAAGA CCCCACCAAC
    ATCCAGCTTT CCAAGCTGCT GAGCAAACAC CAGAAGCACC AGGCCTTTGA GGCGGAACTG
    CATGCCAATG CTGACCGGAT CCGCGGGGTC ATCGACACGG GCAACTCCCT CATCGAGCGT
    GGGGCCTGTG CTGGCAGTGA GGATGCTGTC AAGGCCCGGC TGGCTGCCTT AGCTGACCAG
    TGGCAGTTCC TGGTGCAAAA GTCAGCCGAA AAGAGCCAGA AACTAAAAGA AGCCAACAAG
    CAGCAGAACT TCAACACTGG AATCAAGGAC TTTGACTTCT GGCTTTCTGA GGTGGAGGCC
    CTTCTGGCAT CTGAGGACTA CGGCAAAGAC CTGGCTTCTG TGAACAACTT GCTGAAGAAA
    CATCAACTGC TAGAGGCGGA CATATCTGCC CACGAGGATC GCCTGAAGGA CCTGAATAGC
    CAAGCAGACA GCCTGATGAC CAGCAGTGCC TTCGACACCT CCCAAGTGAA GGATAAGCGG
    GACACCATCA ATGGTCGTTT CCAGAAGATC AAGAACATGG CGGCCTCCCG GCGAGCCAGG
    CTGAATGAAT CCCATCGCCT ACACCAGTTC TTCCGGGACA TGGATGATGA GGAGTCCTGG
    ATCAAGGAGA AAAAGTTGCT GGTGAGCTCA GAGGACTATG GCCGAGACCT TACAGGAGTT
    CAGAACCTGA GGAAGAAACA CAAGCGGCTG GAGGCGGAAC TGGCTGCCCA TGAGCCAGCG
    ATCCAGGGTG TCCTGGACAC TGGGAAGAAG CTGTCTGATG ACAATACCAT TGGCAAGGAG
    GAGATTCAGC AGCGTCTGGC CCAGTTCGTG GAGCACTGGA AGGAGCTGAA GCAGCTGGCG
    GCAGCTCGGG GTCAGCGGCT AGAGGAGTCC TTGGAGTATC AGCAGTTTGT AGCCAATGTG
    GAAGAAGAAG AAGCCTGGAT TAATGAGAAG ATGACCCTGG TAGCCAGTGA GGATTATGGA
    GACACGCTTG CCGCTATCCA GGGCTTACTG AAGAAACATG AAGCTTTTGA GACAGACTTC
    ACTGTGCACA AGGATCGTGT GAACGACGTC TGCAGTAATG GACAAGACCT CATCAAGAAG
    AACAATCACC ACGAGGAGAA CATCTCTTCA AAGATGAAGG GCTTGAATGG CAAAGTGTCA
    GACTTGGAGA AAGCTGCTGC CCAGAGGAAG GCCAAGCTTG AGGAGAACTC GGCCTTCCTT
    CAGTTCAACT GGAAGGCTGA TGTAGTGGAA TCCTGGATCG GTGAGAAGGA AAACAGCCTG
    AAGACAGACG ATTATGGCCG AGACCTGTCC TCCGTGCAGA CTCTCCTCAC CAAACAGGAA
    ACTTTTGACG CTGGTCTACA GGCCTTCCAG CAGGAGGGCA TTGCCAACAT CACTGCCCTC
    AAAGATCAGC TGCTGGCCGC CAAGCACATT CAGTCAAAGG CCATTGAAGC CCGGCACGCC
    TCTCTCATGA AGAGATGGAG CCAGCTTCTG GCCAACTCGG CCACCCGCAA GAAGAAGCTG
    CTGGAGGCAC AGAGTCACTT CCGCAAGGTG GAAGACCTCT TCCTGACCTT CGCCAAAAAG
    GCATCGGCCT TCAACAGCTG GTTTGAAAAC GCCGAAGAGG ATCTGACTGA TCCTGTGCGT
    TGCAACTCCC TGGAGGAGAT CAGAGCTCTG CGTGAGGCCC ACGACGCCTT CCGCTCATCC
    CTCAGCTCTG CCCAGGCCGA CTTCAGCCAG CTGGCTGAGC TGGACCGCCA GATCAAGAGC
    TTCCGTGTGG CCTCTAACCC CTACACCTGG TTCACCATGG AGGCCCTGGA GGAGACGTGG
    AGGAACCTGC AGAAGATCAT CAAGGAGCGG GAGCTGGAGC TGCAAAAGGA GCAGCGGCGA
    CAGGAGGAGA ATGACAAGCT ACGCCAGGAG TTCGCCCAGC ACGCTAACGC CTTCCACCAG
    TGGATACAGG AAACCAGAAC ATATCTCCTC GATGGCATAG CGTATCGTCG GGTCATTCGT
    GTCTATCAGT ATGAAGTTGG GGATGATCTG TCTGGAAGGT CCTGCATGGT GGAAGAATCC
    GGGACCCTGG AATCCCAGCT TGAGGCTACC AAACGCAAGC ACCAGGAGAT TCGAGCCATG
    AGAAGTCAGC TCAAGAAGAT TGAGGACCTG GGGGCTGCCA TGGAGGAGGC CCTGATCCTG
    GATAACAAGT ATACAGAGCA CAGCACCGTG GGCCTGGCCC AACAGTGGGA CCAGCTCGAC
    CAGCTGGGCA TGCGCATGCA GCACAACCTG GAGCAGCAGA TCCAGGCCAG GAACACAACA
    GGAGTGACGG AGGAGGCCCT CAAAGAATTC AGCATGATGT TCAAACACTT CGACAAGGAC
    AAGTCAGGCC GGCTGAACCA CCAGGAGTTC AAGTCCTGCC TGCGCTCCCT GGGTTATGAC
    CTGCCCATGG TGGAGGAAGG GGAGCCTGAC CCCGAGTTCG AGGCAATCCT AGACACCGTG
    GATCCAAACA GGGATGGCCA CGTCTCCCTG CAAGAGTACA TGGCCTTCAT GATCAGCCGT
    GAAACGGAGA ACGTTAAATC CAGTGAGGAG ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC
    GAGGGAAAGC CCTACGTGAC CAAGGAGGAG CTGTATCAGA ACCTGACTCG AGAACAAGCC
    GACTACTGCG TCTCCCACAT GAAGCCCTAC GTGGATGGCA AGGGCCGCGA ACTCCCCACT
    GCCTTCGACT ACGTGGAGTT CACCCGCTCG CTCTTTGTGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972488.1
    CDS119..7603
    Translation

    Target ORF information:

    RefSeq Version XM_014117013.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117013.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGGAGCT GAACGAGCGT TGGCGGTCCC TGCAGCAGCT GGCCGAGGAG
    CGGAGCCAGC TCTTGGGCAG CGCCCATGAA GTACAGAGGT TCCACAGAGA TGCCGATGAA
    ACCAAAGAGT GGATTGAGGA GAAGAATCAG GCCCTAAACA CAGACAACTA TGGCCACGAT
    CTGGCCAGCG TACAAGCTCT GCAGCGCAAG CACGAGGGCT TTGAGAGGGA CCTCGCGGCT
    CTTGGTGACA AGGTCAACTC CCTGGGGGAA ACAGCCGAGC GCCTGATCCA GTCCCACCCG
    GAGTCGGCCG AGGACCTGCA GGAAAAGTGC ACGGAGTTGG ACCAGGCGTG GAGCAGCCTG
    GGGAAGCGCG CAGACCAGCG CAAGGCCAAG CTGGGTGATT CCCACGACCT GCAACGCTTC
    CTTAGTGATT TCCGGGACCT CATGTCTTGG ATCAATGGAA TACGAGGGTT GGTGTCCTCA
    GACGAGTTAG CCAAGGATGT CACCGGAGCA GAAGCTTTGC TGGAACGGCA CCAGGAGCAC
    CGGACAGAAA TCGATGCCAG GGCTGGCACT TTCCAGGCCT TTGAGCAGTT TGGGCAGCAG
    CTGCTGGCTC ATGGGCACTA CGCCAGCCCA GAGGTCAAAG AGAAACTAGA TATTCTAGAT
    CGGGAGCGTG CGGGTCTGGA GAAGGCCTGG GCCCAGCGTA GGATGATGTT GGATCAGTGC
    CTTGAACTGC AGCTGTTTCA TCGGGACTGT GAACAAGCTG AGAACTGGAT GGCCGCCCGG
    GAGGCCTTTC TGAATACTGA GGACAAAGGA GACTCACTGG ACAGCGTGGA GGCTCTGATC
    AAAAAACACG AGGATTTTGA CAAAGCGATC AATGTCCAGG AGGAGAAGAT TGCCGCCTTG
    CAGTCCTTTG CTGACCAACT TATTGCTGCC GGCCACTATG CCAAGGGTGA CATCTGCAGT
    CGGCGCAATG AGGTTTTGGA CAGGTGGCGA CGCCTAAAAG CTCAGATGAT TGAGAAGAGA
    TCGAAGCTGG GAGAATCACA AACCCTCCAG CAGTTCAGCC GGGACGTGGA TGAGATCGAG
    GCTTGGATCA GTGAGAAATT ACAGACTGCG AGTGATGAGT CGTACAAAGA CCCCACCAAC
    ATCCAGCTTT CCAAGCTGCT GAGCAAACAC CAGAAGCACC AGGCCTTTGA GGCGGAACTG
    CATGCCAATG CTGACCGGAT CCGCGGGGTC ATCGACACGG GCAACTCCCT CATCGAGCGT
    GGGGCCTGTG CTGGCAGTGA GGATGCTGTC AAGGCCCGGC TGGCTGCCTT AGCTGACCAG
    TGGCAGTTCC TGGTGCAAAA GTCAGCCGAA AAGAGCCAGA AACTAAAAGA AGCCAACAAG
    CAGCAGAACT TCAACACTGG AATCAAGGAC TTTGACTTCT GGCTTTCTGA GGTGGAGGCC
    CTTCTGGCAT CTGAGGACTA CGGCAAAGAC CTGGCTTCTG TGAACAACTT GCTGAAGAAA
    CATCAACTGC TAGAGGCGGA CATATCTGCC CACGAGGATC GCCTGAAGGA CCTGAATAGC
    CAAGCAGACA GCCTGATGAC CAGCAGTGCC TTCGACACCT CCCAAGTGAA GGATAAGCGG
    GACACCATCA ATGGTCGTTT CCAGAAGATC AAGAACATGG CGGCCTCCCG GCGAGCCAGG
    CTGAATGAAT CCCATCGCCT ACACCAGTTC TTCCGGGACA TGGATGATGA GGAGTCCTGG
    ATCAAGGAGA AAAAGTTGCT GGTGAGCTCA GAGGACTATG GCCGAGACCT TACAGGAGTT
    CAGAACCTGA GGAAGAAACA CAAGCGGCTG GAGGCGGAAC TGGCTGCCCA TGAGCCAGCG
    ATCCAGGGTG TCCTGGACAC TGGGAAGAAG CTGTCTGATG ACAATACCAT TGGCAAGGAG
    GAGATTCAGC AGCGTCTGGC CCAGTTCGTG GAGCACTGGA AGGAGCTGAA GCAGCTGGCG
    GCAGCTCGGG GTCAGCGGCT AGAGGAGTCC TTGGAGTATC AGCAGTTTGT AGCCAATGTG
    GAAGAAGAAG AAGCCTGGAT TAATGAGAAG ATGACCCTGG TAGCCAGTGA GGATTATGGA
    GACACGCTTG CCGCTATCCA GGGCTTACTG AAGAAACATG AAGCTTTTGA GACAGACTTC
    ACTGTGCACA AGGATCGTGT GAACGACGTC TGCAGTAATG GACAAGACCT CATCAAGAAG
    AACAATCACC ACGAGGAGAA CATCTCTTCA AAGATGAAGG GCTTGAATGG CAAAGTGTCA
    GACTTGGAGA AAGCTGCTGC CCAGAGGAAG GCCAAGCTTG AGGAGAACTC GGCCTTCCTT
    CAGTTCAACT GGAAGGCTGA TGTAGTGGAA TCCTGGATCG GTGAGAAGGA AAACAGCCTG
    AAGACAGACG ATTATGGCCG AGACCTGTCC TCCGTGCAGA CTCTCCTCAC CAAACAGGAA
    ACTTTTGACG CTGGTCTACA GGCCTTCCAG CAGGAGGGCA TTGCCAACAT CACTGCCCTC
    AAAGATCAGC TGCTGGCCGC CAAGCACATT CAGTCAAAGG CCATTGAAGC CCGGCACGCC
    TCTCTCATGA AGAGATGGAG CCAGCTTCTG GCCAACTCGG CCACCCGCAA GAAGAAGCTG
    CTGGAGGCAC AGAGTCACTT CCGCAAGGTG GAAGACCTCT TCCTGACCTT CGCCAAAAAG
    GCATCGGCCT TCAACAGCTG GTTTGAAAAC GCCGAAGAGG ATCTGACTGA TCCTGTGCGT
    TGCAACTCCC TGGAGGAGAT CAGAGCTCTG CGTGAGGCCC ACGACGCCTT CCGCTCATCC
    CTCAGCTCTG CCCAGGCCGA CTTCAGCCAG CTGGCTGAGC TGGACCGCCA GATCAAGAGC
    TTCCGTGTGG CCTCTAACCC CTACACCTGG TTCACCATGG AGGCCCTGGA GGAGACGTGG
    AGGAACCTGC AGAAGATCAT CAAGGAGCGG GAGCTGGAGC TGCAAAAGGA GCAGCGGCGA
    CAGGAGGAGA ATGACAAGCT ACGCCAGGAG TTCGCCCAGC ACGCTAACGC CTTCCACCAG
    TGGATACAGG AAACCAGAAC ATATCTCCTC GATGGCATAG CGTATCGTCG GGTCATTCGT
    GTCTATCAGT ATGAAGTTGG GGATGATCTG TCTGGAAGGT CCTGCATGGT GGAAGAATCC
    GGGACCCTGG AATCCCAGCT TGAGGCTACC AAACGCAAGC ACCAGGAGAT TCGAGCCATG
    AGAAGTCAGC TCAAGAAGAT TGAGGACCTG GGGGCTGCCA TGGAGGAGGC CCTGATCCTG
    GATAACAAGT ATACAGAGCA CAGCACCGTG GGCCTGGCCC AACAGTGGGA CCAGCTCGAC
    CAGCTGGGCA TGCGCATGCA GCACAACCTG GAGCAGCAGA TCCAGGCCAG GAACACAACA
    GGAGTGACGG AGGAGGCCCT CAAAGAATTC AGCATGATGT TCAAACACTT CGACAAGGAC
    AAGTCAGGCC GGCTGAACCA CCAGGAGTTC AAGTCCTGCC TGCGCTCCCT GGGTTATGAC
    CTGCCCATGG TGGAGGAAGG GGAGCCTGAC CCCGAGTTCG AGGCAATCCT AGACACCGTG
    GATCCAAACA GGGATGGCCA CGTCTCCCTG CAAGAGTACA TGGCCTTCAT GATCAGCCGT
    GAAACGGAGA ACGTTAAATC CAGTGAGGAG ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC
    GAGGGAAAGC CCTACGTGAC CAAGGAGGAG CTGTATCAGA ACCTGACTCG AGAACAAGCC
    GACTACTGCG TCTCCCACAT GAAGCCCTAC GTGGATGGCA AGGGCCGCGA ACTCCCCACT
    GCCTTCGACT ACGTGGAGTT CACCCGCTCG CTCTTTGTGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38675
    Clone ID Related Accession (Same CDS sequence) XM_014117014.2 , XM_014117014.1
    Accession Version XM_014117014.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGGTCCT GCATGGTGGA AGAATCCGGG ACCCTGGAAT CCCAGCTTGA GGCTACCAAA
    CGCAAGCACC AGGAGATTCG AGCCATGAGA AGTCAGCTCA AGAAGATTGA GGACCTGGGG
    GCTGCCATGG AGGAGGCCCT GATCCTGGAT AACAAGTATA CAGAGCACAG CACCGTGGGC
    CTGGCCCAAC AGTGGGACCA GCTCGACCAG CTGGGCATGC GCATGCAGCA CAACCTGGAG
    CAGCAGATCC AGGCCAGGAA CACAACAGGA GTGACGGAGG AGGCCCTCAA AGAATTCAGC
    ATGATGTTCA AACACTTCGA CAAGGACAAG TCAGGCCGGC TGAACCACCA GGAGTTCAAG
    TCCTGCCTGC GCTCCCTGGG TTATGACCTG CCCATGGTGG AGGAAGGGGA GCCTGACCCC
    GAGTTCGAGG CAATCCTAGA CACCGTGGAT CCAAACAGGG ATGGCCACGT CTCCCTGCAA
    GAGTACATGG CCTTCATGAT CAGCCGTGAA ACGGAGAACG TTAAATCCAG TGAGGAGATT
    GAGAGCGCCT TCCGGGCCCT GAGCTCCGAG GGAAAGCCCT ACGTGACCAA GGAGGAGCTG
    TATCAGAACC TGACTCGAGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG
    GATGGCAAGG GCCGCGAACT CCCCACTGCC TTCGACTACG TGGAGTTCAC CCGCTCGCTC
    TTTGTGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972489.1
    CDS137..7588
    Translation

    Target ORF information:

    RefSeq Version XM_014117014.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117014.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGGTCCT GCATGGTGGA AGAATCCGGG ACCCTGGAAT CCCAGCTTGA GGCTACCAAA
    CGCAAGCACC AGGAGATTCG AGCCATGAGA AGTCAGCTCA AGAAGATTGA GGACCTGGGG
    GCTGCCATGG AGGAGGCCCT GATCCTGGAT AACAAGTATA CAGAGCACAG CACCGTGGGC
    CTGGCCCAAC AGTGGGACCA GCTCGACCAG CTGGGCATGC GCATGCAGCA CAACCTGGAG
    CAGCAGATCC AGGCCAGGAA CACAACAGGA GTGACGGAGG AGGCCCTCAA AGAATTCAGC
    ATGATGTTCA AACACTTCGA CAAGGACAAG TCAGGCCGGC TGAACCACCA GGAGTTCAAG
    TCCTGCCTGC GCTCCCTGGG TTATGACCTG CCCATGGTGG AGGAAGGGGA GCCTGACCCC
    GAGTTCGAGG CAATCCTAGA CACCGTGGAT CCAAACAGGG ATGGCCACGT CTCCCTGCAA
    GAGTACATGG CCTTCATGAT CAGCCGTGAA ACGGAGAACG TTAAATCCAG TGAGGAGATT
    GAGAGCGCCT TCCGGGCCCT GAGCTCCGAG GGAAAGCCCT ACGTGACCAA GGAGGAGCTG
    TATCAGAACC TGACTCGAGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG
    GATGGCAAGG GCCGCGAACT CCCCACTGCC TTCGACTACG TGGAGTTCAC CCGCTCGCTC
    TTTGTGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38675
    Clone ID Related Accession (Same CDS sequence) XM_014117014.2 , XM_014117014.1
    Accession Version XM_014117014.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGGTCCT GCATGGTGGA AGAATCCGGG ACCCTGGAAT CCCAGCTTGA GGCTACCAAA
    CGCAAGCACC AGGAGATTCG AGCCATGAGA AGTCAGCTCA AGAAGATTGA GGACCTGGGG
    GCTGCCATGG AGGAGGCCCT GATCCTGGAT AACAAGTATA CAGAGCACAG CACCGTGGGC
    CTGGCCCAAC AGTGGGACCA GCTCGACCAG CTGGGCATGC GCATGCAGCA CAACCTGGAG
    CAGCAGATCC AGGCCAGGAA CACAACAGGA GTGACGGAGG AGGCCCTCAA AGAATTCAGC
    ATGATGTTCA AACACTTCGA CAAGGACAAG TCAGGCCGGC TGAACCACCA GGAGTTCAAG
    TCCTGCCTGC GCTCCCTGGG TTATGACCTG CCCATGGTGG AGGAAGGGGA GCCTGACCCC
    GAGTTCGAGG CAATCCTAGA CACCGTGGAT CCAAACAGGG ATGGCCACGT CTCCCTGCAA
    GAGTACATGG CCTTCATGAT CAGCCGTGAA ACGGAGAACG TTAAATCCAG TGAGGAGATT
    GAGAGCGCCT TCCGGGCCCT GAGCTCCGAG GGAAAGCCCT ACGTGACCAA GGAGGAGCTG
    TATCAGAACC TGACTCGAGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG
    GATGGCAAGG GCCGCGAACT CCCCACTGCC TTCGACTACG TGGAGTTCAC CCGCTCGCTC
    TTTGTGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972489.1
    CDS119..7570
    Translation

    Target ORF information:

    RefSeq Version XM_014117014.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117014.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGGTCCT GCATGGTGGA AGAATCCGGG ACCCTGGAAT CCCAGCTTGA GGCTACCAAA
    CGCAAGCACC AGGAGATTCG AGCCATGAGA AGTCAGCTCA AGAAGATTGA GGACCTGGGG
    GCTGCCATGG AGGAGGCCCT GATCCTGGAT AACAAGTATA CAGAGCACAG CACCGTGGGC
    CTGGCCCAAC AGTGGGACCA GCTCGACCAG CTGGGCATGC GCATGCAGCA CAACCTGGAG
    CAGCAGATCC AGGCCAGGAA CACAACAGGA GTGACGGAGG AGGCCCTCAA AGAATTCAGC
    ATGATGTTCA AACACTTCGA CAAGGACAAG TCAGGCCGGC TGAACCACCA GGAGTTCAAG
    TCCTGCCTGC GCTCCCTGGG TTATGACCTG CCCATGGTGG AGGAAGGGGA GCCTGACCCC
    GAGTTCGAGG CAATCCTAGA CACCGTGGAT CCAAACAGGG ATGGCCACGT CTCCCTGCAA
    GAGTACATGG CCTTCATGAT CAGCCGTGAA ACGGAGAACG TTAAATCCAG TGAGGAGATT
    GAGAGCGCCT TCCGGGCCCT GAGCTCCGAG GGAAAGCCCT ACGTGACCAA GGAGGAGCTG
    TATCAGAACC TGACTCGAGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG
    GATGGCAAGG GCCGCGAACT CCCCACTGCC TTCGACTACG TGGAGTTCAC CCGCTCGCTC
    TTTGTGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12754
    Clone ID Related Accession (Same CDS sequence) XM_014117012.2 , XM_014117012.1
    Accession Version XM_014117012.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTGGAA
    TCCCAGCTTG AGGCTACCAA ACGCAAGCAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC
    AAGAAGATTG AGGACCTGGG GGCTGCCATG GAGGAGGCCC TGATCCTGGA TAACAAGTAT
    ACAGAGCACA GCACCGTGGG CCTGGCCCAA CAGTGGGACC AGCTCGACCA GCTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG AGTGACGGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCAGGCCGG
    CTGAACCACC AGGAGTTCAA GTCCTGCCTG CGCTCCCTGG GTTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCTGACCC CGAGTTCGAG GCAATCCTAG ACACCGTGGA TCCAAACAGG
    GATGGCCACG TCTCCCTGCA AGAGTACATG GCCTTCATGA TCAGCCGTGA AACGGAGAAC
    GTTAAATCCA GTGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA GGGAAAGCCC
    TACGTGACCA AGGAGGAGCT GTATCAGAAC CTGACTCGAG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGATGGCAAG GGCCGCGAAC TCCCCACTGC CTTCGACTAC
    GTGGAGTTCA CCCGCTCGCT CTTTGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972487.1
    CDS137..7669
    Translation

    Target ORF information:

    RefSeq Version XM_014117012.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117012.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTGGAA
    TCCCAGCTTG AGGCTACCAA ACGCAAGCAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC
    AAGAAGATTG AGGACCTGGG GGCTGCCATG GAGGAGGCCC TGATCCTGGA TAACAAGTAT
    ACAGAGCACA GCACCGTGGG CCTGGCCCAA CAGTGGGACC AGCTCGACCA GCTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG AGTGACGGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCAGGCCGG
    CTGAACCACC AGGAGTTCAA GTCCTGCCTG CGCTCCCTGG GTTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCTGACCC CGAGTTCGAG GCAATCCTAG ACACCGTGGA TCCAAACAGG
    GATGGCCACG TCTCCCTGCA AGAGTACATG GCCTTCATGA TCAGCCGTGA AACGGAGAAC
    GTTAAATCCA GTGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA GGGAAAGCCC
    TACGTGACCA AGGAGGAGCT GTATCAGAAC CTGACTCGAG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGATGGCAAG GGCCGCGAAC TCCCCACTGC CTTCGACTAC
    GTGGAGTTCA CCCGCTCGCT CTTTGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12754
    Clone ID Related Accession (Same CDS sequence) XM_014117012.2 , XM_014117012.1
    Accession Version XM_014117012.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTGGAA
    TCCCAGCTTG AGGCTACCAA ACGCAAGCAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC
    AAGAAGATTG AGGACCTGGG GGCTGCCATG GAGGAGGCCC TGATCCTGGA TAACAAGTAT
    ACAGAGCACA GCACCGTGGG CCTGGCCCAA CAGTGGGACC AGCTCGACCA GCTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG AGTGACGGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCAGGCCGG
    CTGAACCACC AGGAGTTCAA GTCCTGCCTG CGCTCCCTGG GTTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCTGACCC CGAGTTCGAG GCAATCCTAG ACACCGTGGA TCCAAACAGG
    GATGGCCACG TCTCCCTGCA AGAGTACATG GCCTTCATGA TCAGCCGTGA AACGGAGAAC
    GTTAAATCCA GTGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA GGGAAAGCCC
    TACGTGACCA AGGAGGAGCT GTATCAGAAC CTGACTCGAG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGATGGCAAG GGCCGCGAAC TCCCCACTGC CTTCGACTAC
    GTGGAGTTCA CCCGCTCGCT CTTTGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972487.1
    CDS119..7651
    Translation

    Target ORF information:

    RefSeq Version XM_014117012.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117012.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTGGAA
    TCCCAGCTTG AGGCTACCAA ACGCAAGCAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC
    AAGAAGATTG AGGACCTGGG GGCTGCCATG GAGGAGGCCC TGATCCTGGA TAACAAGTAT
    ACAGAGCACA GCACCGTGGG CCTGGCCCAA CAGTGGGACC AGCTCGACCA GCTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG AGTGACGGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCAGGCCGG
    CTGAACCACC AGGAGTTCAA GTCCTGCCTG CGCTCCCTGG GTTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCTGACCC CGAGTTCGAG GCAATCCTAG ACACCGTGGA TCCAAACAGG
    GATGGCCACG TCTCCCTGCA AGAGTACATG GCCTTCATGA TCAGCCGTGA AACGGAGAAC
    GTTAAATCCA GTGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA GGGAAAGCCC
    TACGTGACCA AGGAGGAGCT GTATCAGAAC CTGACTCGAG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGATGGCAAG GGCCGCGAAC TCCCCACTGC CTTCGACTAC
    GTGGAGTTCA CCCGCTCGCT CTTTGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12755
    Clone ID Related Accession (Same CDS sequence) XM_005625282.3 , XM_005625282.2
    Accession Version XM_005625282.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625282.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGAGCAAA
    CACCAGAAGC ACCAGGCCTT TGAGGCGGAA CTGCATGCCA ATGCTGACCG GATCCGCGGG
    GTCATCGACA CGGGCAACTC CCTCATCGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GGCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA AAAGTCAGCC
    GAAAAGAGCC AGAAACTAAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGAATCAAG
    GACTTTGACT TCTGGCTTTC TGAGGTGGAG GCCCTTCTGG CATCTGAGGA CTACGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAG AAACATCAAC TGCTAGAGGC GGACATATCT
    GCCCACGAGG ATCGCCTGAA GGACCTGAAT AGCCAAGCAG ACAGCCTGAT GACCAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAGGATAAG CGGGACACCA TCAATGGTCG TTTCCAGAAG
    ATCAAGAACA TGGCGGCCTC CCGGCGAGCC AGGCTGAATG AATCCCATCG CCTACACCAG
    TTCTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AGAAAAAGTT GCTGGTGAGC
    TCAGAGGACT ATGGCCGAGA CCTTACAGGA GTTCAGAACC TGAGGAAGAA ACACAAGCGG
    CTGGAGGCGG AACTGGCTGC CCATGAGCCA GCGATCCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAATAC CATTGGCAAG GAGGAGATTC AGCAGCGTCT GGCCCAGTTC
    GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCGGCAGCTC GGGGTCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAT GTGGAAGAAG AAGAAGCCTG GATTAATGAG
    AAGATGACCC TGGTAGCCAG TGAGGATTAT GGAGACACGC TTGCCGCTAT CCAGGGCTTA
    CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACTGTGC ACAAGGATCG TGTGAACGAC
    GTCTGCAGTA ATGGACAAGA CCTCATCAAG AAGAACAATC ACCACGAGGA GAACATCTCT
    TCAAAGATGA AGGGCTTGAA TGGCAAAGTG TCAGACTTGG AGAAAGCTGC TGCCCAGAGG
    AAGGCCAAGC TTGAGGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC TGATGTAGTG
    GAATCCTGGA TCGGTGAGAA GGAAAACAGC CTGAAGACAG ACGATTATGG CCGAGACCTG
    TCCTCCGTGC AGACTCTCCT CACCAAACAG GAAACTTTTG ACGCTGGTCT ACAGGCCTTC
    CAGCAGGAGG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC
    ATTCAGTCAA AGGCCATTGA AGCCCGGCAC GCCTCTCTCA TGAAGAGATG GAGCCAGCTT
    CTGGCCAACT CGGCCACCCG CAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG
    GTGGAAGACC TCTTCCTGAC CTTCGCCAAA AAGGCATCGG CCTTCAACAG CTGGTTTGAA
    AACGCCGAAG AGGATCTGAC TGATCCTGTG CGTTGCAACT CCCTGGAGGA GATCAGAGCT
    CTGCGTGAGG CCCACGACGC CTTCCGCTCA TCCCTCAGCT CTGCCCAGGC CGACTTCAGC
    CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGTG TGGCCTCTAA CCCCTACACC
    TGGTTCACCA TGGAGGCCCT GGAGGAGACG TGGAGGAACC TGCAGAAGAT CATCAAGGAG
    CGGGAGCTGG AGCTGCAAAA GGAGCAGCGG CGACAGGAGG AGAATGACAA GCTACGCCAG
    GAGTTCGCCC AGCACGCTAA CGCCTTCCAC CAGTGGATAC AGGAAACCAG AACATATCTC
    CTCGATGGCA TAGCGTATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT
    CTGTCTGGAA GGTCCTGCAT GGTGGAAGAA TCCGGGACCC TGGAATCCCA GCTTGAGGCT
    ACCAAACGCA AGCACCAGGA GATTCGAGCC ATGAGAAGTC AGCTCAAGAA GATTGAGGAC
    CTGGGGGCTG CCATGGAGGA GGCCCTGATC CTGGATAACA AGTATACAGA GCACAGCACC
    GTGGGCCTGG CCCAACAGTG GGACCAGCTC GACCAGCTGG GCATGCGCAT GCAGCACAAC
    CTGGAGCAGC AGATCCAGGC CAGGAACACA ACAGGAGTGA CGGAGGAGGC CCTCAAAGAA
    TTCAGCATGA TGTTCAAACA CTTCGACAAG GACAAGTCAG GCCGGCTGAA CCACCAGGAG
    TTCAAGTCCT GCCTGCGCTC CCTGGGTTAT GACCTGCCCA TGGTGGAGGA AGGGGAGCCT
    GACCCCGAGT TCGAGGCAAT CCTAGACACC GTGGATCCAA ACAGGGATGG CCACGTCTCC
    CTGCAAGAGT ACATGGCCTT CATGATCAGC CGTGAAACGG AGAACGTTAA ATCCAGTGAG
    GAGATTGAGA GCGCCTTCCG GGCCCTGAGC TCCGAGGGAA AGCCCTACGT GACCAAGGAG
    GAGCTGTATC AGAACCTGAC TCGAGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC
    TACGTGGATG GCAAGGGCCG CGAACTCCCC ACTGCCTTCG ACTACGTGGA GTTCACCCGC
    TCGCTCTTTG TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625339.1
    CDS137..7654
    Translation

    Target ORF information:

    RefSeq Version XM_005625282.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625282.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGAGCAAA
    CACCAGAAGC ACCAGGCCTT TGAGGCGGAA CTGCATGCCA ATGCTGACCG GATCCGCGGG
    GTCATCGACA CGGGCAACTC CCTCATCGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GGCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA AAAGTCAGCC
    GAAAAGAGCC AGAAACTAAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGAATCAAG
    GACTTTGACT TCTGGCTTTC TGAGGTGGAG GCCCTTCTGG CATCTGAGGA CTACGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAG AAACATCAAC TGCTAGAGGC GGACATATCT
    GCCCACGAGG ATCGCCTGAA GGACCTGAAT AGCCAAGCAG ACAGCCTGAT GACCAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAGGATAAG CGGGACACCA TCAATGGTCG TTTCCAGAAG
    ATCAAGAACA TGGCGGCCTC CCGGCGAGCC AGGCTGAATG AATCCCATCG CCTACACCAG
    TTCTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AGAAAAAGTT GCTGGTGAGC
    TCAGAGGACT ATGGCCGAGA CCTTACAGGA GTTCAGAACC TGAGGAAGAA ACACAAGCGG
    CTGGAGGCGG AACTGGCTGC CCATGAGCCA GCGATCCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAATAC CATTGGCAAG GAGGAGATTC AGCAGCGTCT GGCCCAGTTC
    GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCGGCAGCTC GGGGTCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAT GTGGAAGAAG AAGAAGCCTG GATTAATGAG
    AAGATGACCC TGGTAGCCAG TGAGGATTAT GGAGACACGC TTGCCGCTAT CCAGGGCTTA
    CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACTGTGC ACAAGGATCG TGTGAACGAC
    GTCTGCAGTA ATGGACAAGA CCTCATCAAG AAGAACAATC ACCACGAGGA GAACATCTCT
    TCAAAGATGA AGGGCTTGAA TGGCAAAGTG TCAGACTTGG AGAAAGCTGC TGCCCAGAGG
    AAGGCCAAGC TTGAGGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC TGATGTAGTG
    GAATCCTGGA TCGGTGAGAA GGAAAACAGC CTGAAGACAG ACGATTATGG CCGAGACCTG
    TCCTCCGTGC AGACTCTCCT CACCAAACAG GAAACTTTTG ACGCTGGTCT ACAGGCCTTC
    CAGCAGGAGG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC
    ATTCAGTCAA AGGCCATTGA AGCCCGGCAC GCCTCTCTCA TGAAGAGATG GAGCCAGCTT
    CTGGCCAACT CGGCCACCCG CAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG
    GTGGAAGACC TCTTCCTGAC CTTCGCCAAA AAGGCATCGG CCTTCAACAG CTGGTTTGAA
    AACGCCGAAG AGGATCTGAC TGATCCTGTG CGTTGCAACT CCCTGGAGGA GATCAGAGCT
    CTGCGTGAGG CCCACGACGC CTTCCGCTCA TCCCTCAGCT CTGCCCAGGC CGACTTCAGC
    CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGTG TGGCCTCTAA CCCCTACACC
    TGGTTCACCA TGGAGGCCCT GGAGGAGACG TGGAGGAACC TGCAGAAGAT CATCAAGGAG
    CGGGAGCTGG AGCTGCAAAA GGAGCAGCGG CGACAGGAGG AGAATGACAA GCTACGCCAG
    GAGTTCGCCC AGCACGCTAA CGCCTTCCAC CAGTGGATAC AGGAAACCAG AACATATCTC
    CTCGATGGCA TAGCGTATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT
    CTGTCTGGAA GGTCCTGCAT GGTGGAAGAA TCCGGGACCC TGGAATCCCA GCTTGAGGCT
    ACCAAACGCA AGCACCAGGA GATTCGAGCC ATGAGAAGTC AGCTCAAGAA GATTGAGGAC
    CTGGGGGCTG CCATGGAGGA GGCCCTGATC CTGGATAACA AGTATACAGA GCACAGCACC
    GTGGGCCTGG CCCAACAGTG GGACCAGCTC GACCAGCTGG GCATGCGCAT GCAGCACAAC
    CTGGAGCAGC AGATCCAGGC CAGGAACACA ACAGGAGTGA CGGAGGAGGC CCTCAAAGAA
    TTCAGCATGA TGTTCAAACA CTTCGACAAG GACAAGTCAG GCCGGCTGAA CCACCAGGAG
    TTCAAGTCCT GCCTGCGCTC CCTGGGTTAT GACCTGCCCA TGGTGGAGGA AGGGGAGCCT
    GACCCCGAGT TCGAGGCAAT CCTAGACACC GTGGATCCAA ACAGGGATGG CCACGTCTCC
    CTGCAAGAGT ACATGGCCTT CATGATCAGC CGTGAAACGG AGAACGTTAA ATCCAGTGAG
    GAGATTGAGA GCGCCTTCCG GGCCCTGAGC TCCGAGGGAA AGCCCTACGT GACCAAGGAG
    GAGCTGTATC AGAACCTGAC TCGAGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC
    TACGTGGATG GCAAGGGCCG CGAACTCCCC ACTGCCTTCG ACTACGTGGA GTTCACCCGC
    TCGCTCTTTG TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12755
    Clone ID Related Accession (Same CDS sequence) XM_005625282.3 , XM_005625282.2
    Accession Version XM_005625282.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625282.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGAGCAAA
    CACCAGAAGC ACCAGGCCTT TGAGGCGGAA CTGCATGCCA ATGCTGACCG GATCCGCGGG
    GTCATCGACA CGGGCAACTC CCTCATCGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GGCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA AAAGTCAGCC
    GAAAAGAGCC AGAAACTAAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGAATCAAG
    GACTTTGACT TCTGGCTTTC TGAGGTGGAG GCCCTTCTGG CATCTGAGGA CTACGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAG AAACATCAAC TGCTAGAGGC GGACATATCT
    GCCCACGAGG ATCGCCTGAA GGACCTGAAT AGCCAAGCAG ACAGCCTGAT GACCAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAGGATAAG CGGGACACCA TCAATGGTCG TTTCCAGAAG
    ATCAAGAACA TGGCGGCCTC CCGGCGAGCC AGGCTGAATG AATCCCATCG CCTACACCAG
    TTCTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AGAAAAAGTT GCTGGTGAGC
    TCAGAGGACT ATGGCCGAGA CCTTACAGGA GTTCAGAACC TGAGGAAGAA ACACAAGCGG
    CTGGAGGCGG AACTGGCTGC CCATGAGCCA GCGATCCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAATAC CATTGGCAAG GAGGAGATTC AGCAGCGTCT GGCCCAGTTC
    GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCGGCAGCTC GGGGTCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAT GTGGAAGAAG AAGAAGCCTG GATTAATGAG
    AAGATGACCC TGGTAGCCAG TGAGGATTAT GGAGACACGC TTGCCGCTAT CCAGGGCTTA
    CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACTGTGC ACAAGGATCG TGTGAACGAC
    GTCTGCAGTA ATGGACAAGA CCTCATCAAG AAGAACAATC ACCACGAGGA GAACATCTCT
    TCAAAGATGA AGGGCTTGAA TGGCAAAGTG TCAGACTTGG AGAAAGCTGC TGCCCAGAGG
    AAGGCCAAGC TTGAGGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC TGATGTAGTG
    GAATCCTGGA TCGGTGAGAA GGAAAACAGC CTGAAGACAG ACGATTATGG CCGAGACCTG
    TCCTCCGTGC AGACTCTCCT CACCAAACAG GAAACTTTTG ACGCTGGTCT ACAGGCCTTC
    CAGCAGGAGG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC
    ATTCAGTCAA AGGCCATTGA AGCCCGGCAC GCCTCTCTCA TGAAGAGATG GAGCCAGCTT
    CTGGCCAACT CGGCCACCCG CAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG
    GTGGAAGACC TCTTCCTGAC CTTCGCCAAA AAGGCATCGG CCTTCAACAG CTGGTTTGAA
    AACGCCGAAG AGGATCTGAC TGATCCTGTG CGTTGCAACT CCCTGGAGGA GATCAGAGCT
    CTGCGTGAGG CCCACGACGC CTTCCGCTCA TCCCTCAGCT CTGCCCAGGC CGACTTCAGC
    CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGTG TGGCCTCTAA CCCCTACACC
    TGGTTCACCA TGGAGGCCCT GGAGGAGACG TGGAGGAACC TGCAGAAGAT CATCAAGGAG
    CGGGAGCTGG AGCTGCAAAA GGAGCAGCGG CGACAGGAGG AGAATGACAA GCTACGCCAG
    GAGTTCGCCC AGCACGCTAA CGCCTTCCAC CAGTGGATAC AGGAAACCAG AACATATCTC
    CTCGATGGCA TAGCGTATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT
    CTGTCTGGAA GGTCCTGCAT GGTGGAAGAA TCCGGGACCC TGGAATCCCA GCTTGAGGCT
    ACCAAACGCA AGCACCAGGA GATTCGAGCC ATGAGAAGTC AGCTCAAGAA GATTGAGGAC
    CTGGGGGCTG CCATGGAGGA GGCCCTGATC CTGGATAACA AGTATACAGA GCACAGCACC
    GTGGGCCTGG CCCAACAGTG GGACCAGCTC GACCAGCTGG GCATGCGCAT GCAGCACAAC
    CTGGAGCAGC AGATCCAGGC CAGGAACACA ACAGGAGTGA CGGAGGAGGC CCTCAAAGAA
    TTCAGCATGA TGTTCAAACA CTTCGACAAG GACAAGTCAG GCCGGCTGAA CCACCAGGAG
    TTCAAGTCCT GCCTGCGCTC CCTGGGTTAT GACCTGCCCA TGGTGGAGGA AGGGGAGCCT
    GACCCCGAGT TCGAGGCAAT CCTAGACACC GTGGATCCAA ACAGGGATGG CCACGTCTCC
    CTGCAAGAGT ACATGGCCTT CATGATCAGC CGTGAAACGG AGAACGTTAA ATCCAGTGAG
    GAGATTGAGA GCGCCTTCCG GGCCCTGAGC TCCGAGGGAA AGCCCTACGT GACCAAGGAG
    GAGCTGTATC AGAACCTGAC TCGAGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC
    TACGTGGATG GCAAGGGCCG CGAACTCCCC ACTGCCTTCG ACTACGTGGA GTTCACCCGC
    TCGCTCTTTG TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625339.1
    CDS119..7636
    Translation

    Target ORF information:

    RefSeq Version XM_005625282.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625282.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGAGCAAA
    CACCAGAAGC ACCAGGCCTT TGAGGCGGAA CTGCATGCCA ATGCTGACCG GATCCGCGGG
    GTCATCGACA CGGGCAACTC CCTCATCGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GGCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA AAAGTCAGCC
    GAAAAGAGCC AGAAACTAAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGAATCAAG
    GACTTTGACT TCTGGCTTTC TGAGGTGGAG GCCCTTCTGG CATCTGAGGA CTACGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAG AAACATCAAC TGCTAGAGGC GGACATATCT
    GCCCACGAGG ATCGCCTGAA GGACCTGAAT AGCCAAGCAG ACAGCCTGAT GACCAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAGGATAAG CGGGACACCA TCAATGGTCG TTTCCAGAAG
    ATCAAGAACA TGGCGGCCTC CCGGCGAGCC AGGCTGAATG AATCCCATCG CCTACACCAG
    TTCTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AGAAAAAGTT GCTGGTGAGC
    TCAGAGGACT ATGGCCGAGA CCTTACAGGA GTTCAGAACC TGAGGAAGAA ACACAAGCGG
    CTGGAGGCGG AACTGGCTGC CCATGAGCCA GCGATCCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAATAC CATTGGCAAG GAGGAGATTC AGCAGCGTCT GGCCCAGTTC
    GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCGGCAGCTC GGGGTCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAT GTGGAAGAAG AAGAAGCCTG GATTAATGAG
    AAGATGACCC TGGTAGCCAG TGAGGATTAT GGAGACACGC TTGCCGCTAT CCAGGGCTTA
    CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACTGTGC ACAAGGATCG TGTGAACGAC
    GTCTGCAGTA ATGGACAAGA CCTCATCAAG AAGAACAATC ACCACGAGGA GAACATCTCT
    TCAAAGATGA AGGGCTTGAA TGGCAAAGTG TCAGACTTGG AGAAAGCTGC TGCCCAGAGG
    AAGGCCAAGC TTGAGGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC TGATGTAGTG
    GAATCCTGGA TCGGTGAGAA GGAAAACAGC CTGAAGACAG ACGATTATGG CCGAGACCTG
    TCCTCCGTGC AGACTCTCCT CACCAAACAG GAAACTTTTG ACGCTGGTCT ACAGGCCTTC
    CAGCAGGAGG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC
    ATTCAGTCAA AGGCCATTGA AGCCCGGCAC GCCTCTCTCA TGAAGAGATG GAGCCAGCTT
    CTGGCCAACT CGGCCACCCG CAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG
    GTGGAAGACC TCTTCCTGAC CTTCGCCAAA AAGGCATCGG CCTTCAACAG CTGGTTTGAA
    AACGCCGAAG AGGATCTGAC TGATCCTGTG CGTTGCAACT CCCTGGAGGA GATCAGAGCT
    CTGCGTGAGG CCCACGACGC CTTCCGCTCA TCCCTCAGCT CTGCCCAGGC CGACTTCAGC
    CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGTG TGGCCTCTAA CCCCTACACC
    TGGTTCACCA TGGAGGCCCT GGAGGAGACG TGGAGGAACC TGCAGAAGAT CATCAAGGAG
    CGGGAGCTGG AGCTGCAAAA GGAGCAGCGG CGACAGGAGG AGAATGACAA GCTACGCCAG
    GAGTTCGCCC AGCACGCTAA CGCCTTCCAC CAGTGGATAC AGGAAACCAG AACATATCTC
    CTCGATGGCA TAGCGTATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT
    CTGTCTGGAA GGTCCTGCAT GGTGGAAGAA TCCGGGACCC TGGAATCCCA GCTTGAGGCT
    ACCAAACGCA AGCACCAGGA GATTCGAGCC ATGAGAAGTC AGCTCAAGAA GATTGAGGAC
    CTGGGGGCTG CCATGGAGGA GGCCCTGATC CTGGATAACA AGTATACAGA GCACAGCACC
    GTGGGCCTGG CCCAACAGTG GGACCAGCTC GACCAGCTGG GCATGCGCAT GCAGCACAAC
    CTGGAGCAGC AGATCCAGGC CAGGAACACA ACAGGAGTGA CGGAGGAGGC CCTCAAAGAA
    TTCAGCATGA TGTTCAAACA CTTCGACAAG GACAAGTCAG GCCGGCTGAA CCACCAGGAG
    TTCAAGTCCT GCCTGCGCTC CCTGGGTTAT GACCTGCCCA TGGTGGAGGA AGGGGAGCCT
    GACCCCGAGT TCGAGGCAAT CCTAGACACC GTGGATCCAA ACAGGGATGG CCACGTCTCC
    CTGCAAGAGT ACATGGCCTT CATGATCAGC CGTGAAACGG AGAACGTTAA ATCCAGTGAG
    GAGATTGAGA GCGCCTTCCG GGCCCTGAGC TCCGAGGGAA AGCCCTACGT GACCAAGGAG
    GAGCTGTATC AGAACCTGAC TCGAGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC
    TACGTGGATG GCAAGGGCCG CGAACTCCCC ACTGCCTTCG ACTACGTGGA GTTCACCCGC
    TCGCTCTTTG TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12756
    Clone ID Related Accession (Same CDS sequence) XM_537823.5 , XM_537823.6
    Accession Version XM_537823.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_537823.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTAG ACAGATACCA CCGCTTCAAG GAGCTCTCAA CCCTTAGGCG CCAGAAGCTT
    GAAGATTCCT ATCGATTCCA GTTTTTCCAG AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAAC TTCAGATCGC GTCAGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGC
    AAGCTTCAGA AGCATCAGGC CTTCGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT
    AAACTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCGTC TGAAACCATA
    AGGACCCGTT TAATGGAGCT GCACCGCCAG TGGGAGCTGC TTTTGGAGAA GATGCGAGAG
    AAGGGAGTCA AACTGCTGCA GGCCCAGAAG CTGGTGCAGT ATTTGCGAGA GTGTGAGGAT
    GTGATGGACT GGATCAACGA CAAGGAAGCA ATTGTGACTT CTGAGGAGCT GGGCCAAGAC
    CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCC
    CATGAAGAAA GAGTTAATGA AGTTAATCAG TTTGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCCGAGGAGG AGACCATCAA GACCAAGCAA GATGAAGTAA ATGCGGCCTG GCAGCGGCTG
    AAGGGACTGG CCCTTCAGAG ACAGGGGAAG CTCTTCGGAG CTGCTGAGGT TCAGCGCTTT
    AACAGGGATG TGGATGAAAC CATCAGCTGG ATTAAAGAAA AGGAGCAATT AATGGCCTCT
    GACGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TGCGGAAGCA CGAAGGCTTG
    GAGAGAGATC TTGCTGCTTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCCGACCGC
    CTGCAGCAGT CCCACCCTCT GAGTGCTGCA CAGATTCAAG TGAAGCGAGA GGAGCTGATT
    GCCAACTGGG AGCAGATCCG GACTTTGGCA GCTGAGAGAC ATGCCCGCCT CAATGACTCC
    TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTTA CCAGCTGGGT AACTGAGATG
    AAAGCCCTTA TCAATGCAGA TGAGCTTGCC AATGATGTGG CCGGCGCCGA AGCCTTGCTG
    GACAGGCATC AAGAACACAA GGGTGAGATT GATGCTCACG AGGACAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCTGCT GGGCATTATG CCTCAGATGA AGTGAGGGAG
    AAGCTGAGCA TCCTGGCTGA GGAGAGGGCC GCCCTGCTGG AGCTGTGGGA GCTGCGCCGG
    CAGCAGTATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCCTTCCTG CTGAATGAGG ACTTGGGAGA CTCTCTGGAT
    AGCGTGGAGG CGCTCCTCAA GAAGCATGAG GACTTTGAGA AATCCCTCAG CGCCCAGGAG
    GAAAAAATTA CAGCACTGGA TGAATTTGCA ACCAAGCTGA TTCAGAACAA CCACTACGCC
    ATGGAAGACG TGGCTACCCG CCGAGATGCG CTCCTGAGCC GCCGCAACGC TCTTCATGAA
    AGAGCCATGT GTCGTCGCGC CCAACTGGCA GACTCTTTCC ACCTGCAGCA ATTTTTCCGT
    GACTCTGATG AGCTCAAGAG CTGGGTCAAT GAGAAGATGA AAACCGCCAC AGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG
    CTCTCGGCCA ACCAGAGCCG CATTGACGCC CTGGAGAAAG CAGGGCAAAA GCTGATTGAT
    GTCAACCACT ATGCCAAAGA TGAGGTAGCA GCTCGTATGA ACGAGGTCAT CAGTTTGTGG
    AAGAAATTGC TTGAAGCCAC TGAACTGAAG GGGATAAAGC TTCGTGAGGC GAACCAGCAG
    CAGCAGTTCA ACCGCAATGT TGAGGATATT GAGCTATGGC TGTATGAAGT GGAAGGTCAC
    TTGGCTTCAG ACGATTATGG CAAAGACCTT ACCAATGTCC AGAACCTCCA GAAGAAGCAT
    GCCCTGCTAG AGGCCGATGT GGCTGCTCAC CAGGATCGAA TCGATGGCAT CACCATTCAG
    GCCCGCCAGT TCCAGGATGC TGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAA
    GCCCTTGTGG CTCGCTATGA GGCTCTCAAA GAACCCATGA TTGCCCGAAA GCAGAAGCTG
    GCTGATTCTC TACGCTTGCA GCAGCTCTTC CGTGATGTTG AGGATGAGGA GACGTGGATT
    AGGGAGAAAG AGCCCATCGC TGCGTCCACC AACAGAGGCA AAGATTTGAT TGGAGTCCAA
    AATCTGCTGA AGAAACACCA AGCCCTACAA GCAGAAATTG CTGGGCATGA ACCCCGCATC
    AAGGCGGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC CGCTGAGGAT
    GTGAAGGCTA AGCTCAGCGA ACTGAACCAG AAGTGGGAGT CCCTCAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA AGACTCCTTG CAGGCCCAGC AGTACTTTGC TGACGCCAAC
    GAGGCAGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACTGA CTACGGGAAG
    GACGAGGACT CCGCTGAGGC TCTACTGAAG AAACATGAAG CTTTGATGTC AGATCTCAGC
    GCCTACGGCA GCAGCATCCA GGCCCTGCGG GACCAGGCTC AGTCCTGCCG GCAACAAGTG
    GCCCCCATGG ATGACGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG
    AAGAGCCCTC GAGAGGTTAC CATGAAGAAA GGGGACATTC TCACTTTACT CAACAGCACC
    AACAAGGACT GGTGGAAGGT GGAAGTGAAC GACCGTCAGG GTTTCGTGCC AGCCGCGTAT
    GTGAAGAAGC TGGACCCTGC CCAGTCTGCT TCCCGAGAGA ACCTTCTGGA GGAGCAGGGC
    AGCATTGCTG TGCGGCAGGA GCAGATTGAC CACCAGACGC GCATAACTAA GGAGGCCGGC
    AGTGTGTCTC TGCGTATGAA ACAGGTGGAG GAACTGTATC ACTCTCTGCT GGAGCTGGGC
    GAGAAGCGCA AGGGCATGTT GGAGAAGAGC TGTAAGCGGT TCATGTTGTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAACGAGAAG GAGGCAGCTC TGACCAGCGA GGAGGTGGGC
    GCTGACCTGG AGCAGGTCGA AGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG
    AAGGCCAATG AGTCACGGCT GAAAGACATT AACAAGGTGG CTGAAGACCT GGAGTCTGAA
    GGTCTGATGG CTGAGGAGGT GCAGGCAGTA CAGCAGCAGG AGGTGTATGG CATGATGCCC
    AGGGATGACA CGGATTCCAA GACTGCATCC CCTTGGAAGT CTGCTCGTCT GATGGTTCAT
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GTTGGCGGTC CCTGCAGCAG
    CTGGCCGAGG AGCGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA
    GATGCCGATG AAACCAAAGA GTGGATTGAG GAGAAGAATC AGGCCCTAAA CACAGACAAC
    TATGGCCACG ATCTGGCCAG CGTACAAGCT CTGCAGCGCA AGCACGAGGG CTTTGAGAGG
    GACCTCGCGG CTCTTGGTGA CAAGGTCAAC TCCCTGGGGG AAACAGCCGA GCGCCTGATC
    CAGTCCCACC CGGAGTCGGC CGAGGACCTG CAGGAAAAGT GCACGGAGTT GGACCAGGCG
    TGGAGCAGCC TGGGGAAGCG CGCAGACCAG CGCAAGGCCA AGCTGGGTGA TTCCCACGAC
    CTGCAACGCT TCCTTAGTGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG
    TTGGTGTCCT CAGACGAGTT AGCCAAGGAT GTCACCGGAG CAGAAGCTTT GCTGGAACGG
    CACCAGGAGC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC CTTTGAGCAG
    TTTGGGCAGC AGCTGCTGGC TCATGGGCAC TACGCCAGCC CAGAGGTCAA AGAGAAACTA
    GATATTCTAG ATCGGGAGCG TGCGGGTCTG GAGAAGGCCT GGGCCCAGCG TAGGATGATG
    TTGGATCAGT GCCTTGAACT GCAGCTGTTT CATCGGGACT GTGAACAAGC TGAGAACTGG
    ATGGCCGCCC GGGAGGCCTT TCTGAATACT GAGGACAAAG GAGACTCACT GGACAGCGTG
    GAGGCTCTGA TCAAAAAACA CGAGGATTTT GACAAAGCGA TCAATGTCCA GGAGGAGAAG
    ATTGCCGCCT TGCAGTCCTT TGCTGACCAA CTTATTGCTG CCGGCCACTA TGCCAAGGGT
    GACATCTGCA GTCGGCGCAA TGAGGTTTTG GACAGGTGGC GACGCCTAAA AGCTCAGATG
    ATTGAGAAGA GATCGAAGCT GGGAGAATCA CAAACCCTCC AGCAGTTCAG CCGGGACGTG
    GATGAGATCG AGGCTTGGAT CAGTGAGAAA TTACAGACTG CGAGTGATGA GTCGTACAAA
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAAC ACCAGAAGCA CCAGGCCTTT
    GAGGCGGAAC TGCATGCCAA TGCTGACCGG ATCCGCGGGG TCATCGACAC GGGCAACTCC
    CTCATCGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG GCTGGCTGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAA AAGTCAGCCG AAAAGAGCCA GAAACTAAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGAATCAAGG ACTTTGACTT CTGGCTTTCT
    GAGGTGGAGG CCCTTCTGGC ATCTGAGGAC TACGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTGAAGA AACATCAACT GCTAGAGGCG GACATATCTG CCCACGAGGA TCGCCTGAAG
    GACCTGAATA GCCAAGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTG
    AAGGATAAGC GGGACACCAT CAATGGTCGT TTCCAGAAGA TCAAGAACAT GGCGGCCTCC
    CGGCGAGCCA GGCTGAATGA ATCCCATCGC CTACACCAGT TCTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA GAAAAAGTTG CTGGTGAGCT CAGAGGACTA TGGCCGAGAC
    CTTACAGGAG TTCAGAACCT GAGGAAGAAA CACAAGCGGC TGGAGGCGGA ACTGGCTGCC
    CATGAGCCAG CGATCCAGGG TGTCCTGGAC ACTGGGAAGA AGCTGTCTGA TGACAATACC
    ATTGGCAAGG AGGAGATTCA GCAGCGTCTG GCCCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CGGCAGCTCG GGGTCAGCGG CTAGAGGAGT CCTTGGAGTA TCAGCAGTTT
    GTAGCCAATG TGGAAGAAGA AGAAGCCTGG ATTAATGAGA AGATGACCCT GGTAGCCAGT
    GAGGATTATG GAGACACGCT TGCCGCTATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT
    GAGACAGACT TCACTGTGCA CAAGGATCGT GTGAACGACG TCTGCAGTAA TGGACAAGAC
    CTCATCAAGA AGAACAATCA CCACGAGGAG AACATCTCTT CAAAGATGAA GGGCTTGAAT
    GGCAAAGTGT CAGACTTGGA GAAAGCTGCT GCCCAGAGGA AGGCCAAGCT TGAGGAGAAC
    TCGGCCTTCC TTCAGTTCAA CTGGAAGGCT GATGTAGTGG AATCCTGGAT CGGTGAGAAG
    GAAAACAGCC TGAAGACAGA CGATTATGGC CGAGACCTGT CCTCCGTGCA GACTCTCCTC
    ACCAAACAGG AAACTTTTGA CGCTGGTCTA CAGGCCTTCC AGCAGGAGGG CATTGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACA TTCAGTCAAA GGCCATTGAA
    GCCCGGCACG CCTCTCTCAT GAAGAGATGG AGCCAGCTTC TGGCCAACTC GGCCACCCGC
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAAGACCT CTTCCTGACC
    TTCGCCAAAA AGGCATCGGC CTTCAACAGC TGGTTTGAAA ACGCCGAAGA GGATCTGACT
    GATCCTGTGC GTTGCAACTC CCTGGAGGAG ATCAGAGCTC TGCGTGAGGC CCACGACGCC
    TTCCGCTCAT CCCTCAGCTC TGCCCAGGCC GACTTCAGCC AGCTGGCTGA GCTGGACCGC
    CAGATCAAGA GCTTCCGTGT GGCCTCTAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACGT GGAGGAACCT GCAGAAGATC ATCAAGGAGC GGGAGCTGGA GCTGCAAAAG
    GAGCAGCGGC GACAGGAGGA GAATGACAAG CTACGCCAGG AGTTCGCCCA GCACGCTAAC
    GCCTTCCACC AGTGGATACA GGAAACCAGA ACATATCTCC TCGATGGCAT AGCGTATCGT
    CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG
    GTGGAAGAAT CCGGGACCCT GGAATCCCAG CTTGAGGCTA CCAAACGCAA GCACCAGGAG
    ATTCGAGCCA TGAGAAGTCA GCTCAAGAAG ATTGAGGACC TGGGGGCTGC CATGGAGGAG
    GCCCTGATCC TGGATAACAA GTATACAGAG CACAGCACCG TGGGCCTGGC CCAACAGTGG
    GACCAGCTCG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC
    AGGAACACAA CAGGAGTGAC GGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC
    TTCGACAAGG ACAAGTCAGG CCGGCTGAAC CACCAGGAGT TCAAGTCCTG CCTGCGCTCC
    CTGGGTTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCTG ACCCCGAGTT CGAGGCAATC
    CTAGACACCG TGGATCCAAA CAGGGATGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTC
    ATGATCAGCC GTGAAACGGA GAACGTTAAA TCCAGTGAGG AGATTGAGAG CGCCTTCCGG
    GCCCTGAGCT CCGAGGGAAA GCCCTACGTG ACCAAGGAGG AGCTGTATCA GAACCTGACT
    CGAGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGATGG CAAGGGCCGC
    GAACTCCCCA CTGCCTTCGA CTACGTGGAG TTCACCCGCT CGCTCTTTGT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_537823.4
    CDS109..7605
    Translation

    Target ORF information:

    RefSeq Version XM_537823.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_537823.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTAG ACAGATACCA CCGCTTCAAG GAGCTCTCAA CCCTTAGGCG CCAGAAGCTT
    GAAGATTCCT ATCGATTCCA GTTTTTCCAG AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAAC TTCAGATCGC GTCAGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGC
    AAGCTTCAGA AGCATCAGGC CTTCGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT
    AAACTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCGTC TGAAACCATA
    AGGACCCGTT TAATGGAGCT GCACCGCCAG TGGGAGCTGC TTTTGGAGAA GATGCGAGAG
    AAGGGAGTCA AACTGCTGCA GGCCCAGAAG CTGGTGCAGT ATTTGCGAGA GTGTGAGGAT
    GTGATGGACT GGATCAACGA CAAGGAAGCA ATTGTGACTT CTGAGGAGCT GGGCCAAGAC
    CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCC
    CATGAAGAAA GAGTTAATGA AGTTAATCAG TTTGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCCGAGGAGG AGACCATCAA GACCAAGCAA GATGAAGTAA ATGCGGCCTG GCAGCGGCTG
    AAGGGACTGG CCCTTCAGAG ACAGGGGAAG CTCTTCGGAG CTGCTGAGGT TCAGCGCTTT
    AACAGGGATG TGGATGAAAC CATCAGCTGG ATTAAAGAAA AGGAGCAATT AATGGCCTCT
    GACGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TGCGGAAGCA CGAAGGCTTG
    GAGAGAGATC TTGCTGCTTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCCGACCGC
    CTGCAGCAGT CCCACCCTCT GAGTGCTGCA CAGATTCAAG TGAAGCGAGA GGAGCTGATT
    GCCAACTGGG AGCAGATCCG GACTTTGGCA GCTGAGAGAC ATGCCCGCCT CAATGACTCC
    TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTTA CCAGCTGGGT AACTGAGATG
    AAAGCCCTTA TCAATGCAGA TGAGCTTGCC AATGATGTGG CCGGCGCCGA AGCCTTGCTG
    GACAGGCATC AAGAACACAA GGGTGAGATT GATGCTCACG AGGACAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCTGCT GGGCATTATG CCTCAGATGA AGTGAGGGAG
    AAGCTGAGCA TCCTGGCTGA GGAGAGGGCC GCCCTGCTGG AGCTGTGGGA GCTGCGCCGG
    CAGCAGTATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCCTTCCTG CTGAATGAGG ACTTGGGAGA CTCTCTGGAT
    AGCGTGGAGG CGCTCCTCAA GAAGCATGAG GACTTTGAGA AATCCCTCAG CGCCCAGGAG
    GAAAAAATTA CAGCACTGGA TGAATTTGCA ACCAAGCTGA TTCAGAACAA CCACTACGCC
    ATGGAAGACG TGGCTACCCG CCGAGATGCG CTCCTGAGCC GCCGCAACGC TCTTCATGAA
    AGAGCCATGT GTCGTCGCGC CCAACTGGCA GACTCTTTCC ACCTGCAGCA ATTTTTCCGT
    GACTCTGATG AGCTCAAGAG CTGGGTCAAT GAGAAGATGA AAACCGCCAC AGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG
    CTCTCGGCCA ACCAGAGCCG CATTGACGCC CTGGAGAAAG CAGGGCAAAA GCTGATTGAT
    GTCAACCACT ATGCCAAAGA TGAGGTAGCA GCTCGTATGA ACGAGGTCAT CAGTTTGTGG
    AAGAAATTGC TTGAAGCCAC TGAACTGAAG GGGATAAAGC TTCGTGAGGC GAACCAGCAG
    CAGCAGTTCA ACCGCAATGT TGAGGATATT GAGCTATGGC TGTATGAAGT GGAAGGTCAC
    TTGGCTTCAG ACGATTATGG CAAAGACCTT ACCAATGTCC AGAACCTCCA GAAGAAGCAT
    GCCCTGCTAG AGGCCGATGT GGCTGCTCAC CAGGATCGAA TCGATGGCAT CACCATTCAG
    GCCCGCCAGT TCCAGGATGC TGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAA
    GCCCTTGTGG CTCGCTATGA GGCTCTCAAA GAACCCATGA TTGCCCGAAA GCAGAAGCTG
    GCTGATTCTC TACGCTTGCA GCAGCTCTTC CGTGATGTTG AGGATGAGGA GACGTGGATT
    AGGGAGAAAG AGCCCATCGC TGCGTCCACC AACAGAGGCA AAGATTTGAT TGGAGTCCAA
    AATCTGCTGA AGAAACACCA AGCCCTACAA GCAGAAATTG CTGGGCATGA ACCCCGCATC
    AAGGCGGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC CGCTGAGGAT
    GTGAAGGCTA AGCTCAGCGA ACTGAACCAG AAGTGGGAGT CCCTCAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA AGACTCCTTG CAGGCCCAGC AGTACTTTGC TGACGCCAAC
    GAGGCAGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACTGA CTACGGGAAG
    GACGAGGACT CCGCTGAGGC TCTACTGAAG AAACATGAAG CTTTGATGTC AGATCTCAGC
    GCCTACGGCA GCAGCATCCA GGCCCTGCGG GACCAGGCTC AGTCCTGCCG GCAACAAGTG
    GCCCCCATGG ATGACGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG
    AAGAGCCCTC GAGAGGTTAC CATGAAGAAA GGGGACATTC TCACTTTACT CAACAGCACC
    AACAAGGACT GGTGGAAGGT GGAAGTGAAC GACCGTCAGG GTTTCGTGCC AGCCGCGTAT
    GTGAAGAAGC TGGACCCTGC CCAGTCTGCT TCCCGAGAGA ACCTTCTGGA GGAGCAGGGC
    AGCATTGCTG TGCGGCAGGA GCAGATTGAC CACCAGACGC GCATAACTAA GGAGGCCGGC
    AGTGTGTCTC TGCGTATGAA ACAGGTGGAG GAACTGTATC ACTCTCTGCT GGAGCTGGGC
    GAGAAGCGCA AGGGCATGTT GGAGAAGAGC TGTAAGCGGT TCATGTTGTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAACGAGAAG GAGGCAGCTC TGACCAGCGA GGAGGTGGGC
    GCTGACCTGG AGCAGGTCGA AGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG
    AAGGCCAATG AGTCACGGCT GAAAGACATT AACAAGGTGG CTGAAGACCT GGAGTCTGAA
    GGTCTGATGG CTGAGGAGGT GCAGGCAGTA CAGCAGCAGG AGGTGTATGG CATGATGCCC
    AGGGATGACA CGGATTCCAA GACTGCATCC CCTTGGAAGT CTGCTCGTCT GATGGTTCAT
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GTTGGCGGTC CCTGCAGCAG
    CTGGCCGAGG AGCGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA
    GATGCCGATG AAACCAAAGA GTGGATTGAG GAGAAGAATC AGGCCCTAAA CACAGACAAC
    TATGGCCACG ATCTGGCCAG CGTACAAGCT CTGCAGCGCA AGCACGAGGG CTTTGAGAGG
    GACCTCGCGG CTCTTGGTGA CAAGGTCAAC TCCCTGGGGG AAACAGCCGA GCGCCTGATC
    CAGTCCCACC CGGAGTCGGC CGAGGACCTG CAGGAAAAGT GCACGGAGTT GGACCAGGCG
    TGGAGCAGCC TGGGGAAGCG CGCAGACCAG CGCAAGGCCA AGCTGGGTGA TTCCCACGAC
    CTGCAACGCT TCCTTAGTGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG
    TTGGTGTCCT CAGACGAGTT AGCCAAGGAT GTCACCGGAG CAGAAGCTTT GCTGGAACGG
    CACCAGGAGC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC CTTTGAGCAG
    TTTGGGCAGC AGCTGCTGGC TCATGGGCAC TACGCCAGCC CAGAGGTCAA AGAGAAACTA
    GATATTCTAG ATCGGGAGCG TGCGGGTCTG GAGAAGGCCT GGGCCCAGCG TAGGATGATG
    TTGGATCAGT GCCTTGAACT GCAGCTGTTT CATCGGGACT GTGAACAAGC TGAGAACTGG
    ATGGCCGCCC GGGAGGCCTT TCTGAATACT GAGGACAAAG GAGACTCACT GGACAGCGTG
    GAGGCTCTGA TCAAAAAACA CGAGGATTTT GACAAAGCGA TCAATGTCCA GGAGGAGAAG
    ATTGCCGCCT TGCAGTCCTT TGCTGACCAA CTTATTGCTG CCGGCCACTA TGCCAAGGGT
    GACATCTGCA GTCGGCGCAA TGAGGTTTTG GACAGGTGGC GACGCCTAAA AGCTCAGATG
    ATTGAGAAGA GATCGAAGCT GGGAGAATCA CAAACCCTCC AGCAGTTCAG CCGGGACGTG
    GATGAGATCG AGGCTTGGAT CAGTGAGAAA TTACAGACTG CGAGTGATGA GTCGTACAAA
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAAC ACCAGAAGCA CCAGGCCTTT
    GAGGCGGAAC TGCATGCCAA TGCTGACCGG ATCCGCGGGG TCATCGACAC GGGCAACTCC
    CTCATCGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG GCTGGCTGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAA AAGTCAGCCG AAAAGAGCCA GAAACTAAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGAATCAAGG ACTTTGACTT CTGGCTTTCT
    GAGGTGGAGG CCCTTCTGGC ATCTGAGGAC TACGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTGAAGA AACATCAACT GCTAGAGGCG GACATATCTG CCCACGAGGA TCGCCTGAAG
    GACCTGAATA GCCAAGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTG
    AAGGATAAGC GGGACACCAT CAATGGTCGT TTCCAGAAGA TCAAGAACAT GGCGGCCTCC
    CGGCGAGCCA GGCTGAATGA ATCCCATCGC CTACACCAGT TCTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA GAAAAAGTTG CTGGTGAGCT CAGAGGACTA TGGCCGAGAC
    CTTACAGGAG TTCAGAACCT GAGGAAGAAA CACAAGCGGC TGGAGGCGGA ACTGGCTGCC
    CATGAGCCAG CGATCCAGGG TGTCCTGGAC ACTGGGAAGA AGCTGTCTGA TGACAATACC
    ATTGGCAAGG AGGAGATTCA GCAGCGTCTG GCCCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CGGCAGCTCG GGGTCAGCGG CTAGAGGAGT CCTTGGAGTA TCAGCAGTTT
    GTAGCCAATG TGGAAGAAGA AGAAGCCTGG ATTAATGAGA AGATGACCCT GGTAGCCAGT
    GAGGATTATG GAGACACGCT TGCCGCTATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT
    GAGACAGACT TCACTGTGCA CAAGGATCGT GTGAACGACG TCTGCAGTAA TGGACAAGAC
    CTCATCAAGA AGAACAATCA CCACGAGGAG AACATCTCTT CAAAGATGAA GGGCTTGAAT
    GGCAAAGTGT CAGACTTGGA GAAAGCTGCT GCCCAGAGGA AGGCCAAGCT TGAGGAGAAC
    TCGGCCTTCC TTCAGTTCAA CTGGAAGGCT GATGTAGTGG AATCCTGGAT CGGTGAGAAG
    GAAAACAGCC TGAAGACAGA CGATTATGGC CGAGACCTGT CCTCCGTGCA GACTCTCCTC
    ACCAAACAGG AAACTTTTGA CGCTGGTCTA CAGGCCTTCC AGCAGGAGGG CATTGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACA TTCAGTCAAA GGCCATTGAA
    GCCCGGCACG CCTCTCTCAT GAAGAGATGG AGCCAGCTTC TGGCCAACTC GGCCACCCGC
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAAGACCT CTTCCTGACC
    TTCGCCAAAA AGGCATCGGC CTTCAACAGC TGGTTTGAAA ACGCCGAAGA GGATCTGACT
    GATCCTGTGC GTTGCAACTC CCTGGAGGAG ATCAGAGCTC TGCGTGAGGC CCACGACGCC
    TTCCGCTCAT CCCTCAGCTC TGCCCAGGCC GACTTCAGCC AGCTGGCTGA GCTGGACCGC
    CAGATCAAGA GCTTCCGTGT GGCCTCTAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACGT GGAGGAACCT GCAGAAGATC ATCAAGGAGC GGGAGCTGGA GCTGCAAAAG
    GAGCAGCGGC GACAGGAGGA GAATGACAAG CTACGCCAGG AGTTCGCCCA GCACGCTAAC
    GCCTTCCACC AGTGGATACA GGAAACCAGA ACATATCTCC TCGATGGCAT AGCGTATCGT
    CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG
    GTGGAAGAAT CCGGGACCCT GGAATCCCAG CTTGAGGCTA CCAAACGCAA GCACCAGGAG
    ATTCGAGCCA TGAGAAGTCA GCTCAAGAAG ATTGAGGACC TGGGGGCTGC CATGGAGGAG
    GCCCTGATCC TGGATAACAA GTATACAGAG CACAGCACCG TGGGCCTGGC CCAACAGTGG
    GACCAGCTCG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC
    AGGAACACAA CAGGAGTGAC GGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC
    TTCGACAAGG ACAAGTCAGG CCGGCTGAAC CACCAGGAGT TCAAGTCCTG CCTGCGCTCC
    CTGGGTTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCTG ACCCCGAGTT CGAGGCAATC
    CTAGACACCG TGGATCCAAA CAGGGATGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTC
    ATGATCAGCC GTGAAACGGA GAACGTTAAA TCCAGTGAGG AGATTGAGAG CGCCTTCCGG
    GCCCTGAGCT CCGAGGGAAA GCCCTACGTG ACCAAGGAGG AGCTGTATCA GAACCTGACT
    CGAGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGATGG CAAGGGCCGC
    GAACTCCCCA CTGCCTTCGA CTACGTGGAG TTCACCCGCT CGCTCTTTGT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12756
    Clone ID Related Accession (Same CDS sequence) XM_537823.5 , XM_537823.6
    Accession Version XM_537823.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_537823.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTAG ACAGATACCA CCGCTTCAAG GAGCTCTCAA CCCTTAGGCG CCAGAAGCTT
    GAAGATTCCT ATCGATTCCA GTTTTTCCAG AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAAC TTCAGATCGC GTCAGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGC
    AAGCTTCAGA AGCATCAGGC CTTCGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT
    AAACTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCGTC TGAAACCATA
    AGGACCCGTT TAATGGAGCT GCACCGCCAG TGGGAGCTGC TTTTGGAGAA GATGCGAGAG
    AAGGGAGTCA AACTGCTGCA GGCCCAGAAG CTGGTGCAGT ATTTGCGAGA GTGTGAGGAT
    GTGATGGACT GGATCAACGA CAAGGAAGCA ATTGTGACTT CTGAGGAGCT GGGCCAAGAC
    CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCC
    CATGAAGAAA GAGTTAATGA AGTTAATCAG TTTGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCCGAGGAGG AGACCATCAA GACCAAGCAA GATGAAGTAA ATGCGGCCTG GCAGCGGCTG
    AAGGGACTGG CCCTTCAGAG ACAGGGGAAG CTCTTCGGAG CTGCTGAGGT TCAGCGCTTT
    AACAGGGATG TGGATGAAAC CATCAGCTGG ATTAAAGAAA AGGAGCAATT AATGGCCTCT
    GACGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TGCGGAAGCA CGAAGGCTTG
    GAGAGAGATC TTGCTGCTTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCCGACCGC
    CTGCAGCAGT CCCACCCTCT GAGTGCTGCA CAGATTCAAG TGAAGCGAGA GGAGCTGATT
    GCCAACTGGG AGCAGATCCG GACTTTGGCA GCTGAGAGAC ATGCCCGCCT CAATGACTCC
    TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTTA CCAGCTGGGT AACTGAGATG
    AAAGCCCTTA TCAATGCAGA TGAGCTTGCC AATGATGTGG CCGGCGCCGA AGCCTTGCTG
    GACAGGCATC AAGAACACAA GGGTGAGATT GATGCTCACG AGGACAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCTGCT GGGCATTATG CCTCAGATGA AGTGAGGGAG
    AAGCTGAGCA TCCTGGCTGA GGAGAGGGCC GCCCTGCTGG AGCTGTGGGA GCTGCGCCGG
    CAGCAGTATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCCTTCCTG CTGAATGAGG ACTTGGGAGA CTCTCTGGAT
    AGCGTGGAGG CGCTCCTCAA GAAGCATGAG GACTTTGAGA AATCCCTCAG CGCCCAGGAG
    GAAAAAATTA CAGCACTGGA TGAATTTGCA ACCAAGCTGA TTCAGAACAA CCACTACGCC
    ATGGAAGACG TGGCTACCCG CCGAGATGCG CTCCTGAGCC GCCGCAACGC TCTTCATGAA
    AGAGCCATGT GTCGTCGCGC CCAACTGGCA GACTCTTTCC ACCTGCAGCA ATTTTTCCGT
    GACTCTGATG AGCTCAAGAG CTGGGTCAAT GAGAAGATGA AAACCGCCAC AGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG
    CTCTCGGCCA ACCAGAGCCG CATTGACGCC CTGGAGAAAG CAGGGCAAAA GCTGATTGAT
    GTCAACCACT ATGCCAAAGA TGAGGTAGCA GCTCGTATGA ACGAGGTCAT CAGTTTGTGG
    AAGAAATTGC TTGAAGCCAC TGAACTGAAG GGGATAAAGC TTCGTGAGGC GAACCAGCAG
    CAGCAGTTCA ACCGCAATGT TGAGGATATT GAGCTATGGC TGTATGAAGT GGAAGGTCAC
    TTGGCTTCAG ACGATTATGG CAAAGACCTT ACCAATGTCC AGAACCTCCA GAAGAAGCAT
    GCCCTGCTAG AGGCCGATGT GGCTGCTCAC CAGGATCGAA TCGATGGCAT CACCATTCAG
    GCCCGCCAGT TCCAGGATGC TGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAA
    GCCCTTGTGG CTCGCTATGA GGCTCTCAAA GAACCCATGA TTGCCCGAAA GCAGAAGCTG
    GCTGATTCTC TACGCTTGCA GCAGCTCTTC CGTGATGTTG AGGATGAGGA GACGTGGATT
    AGGGAGAAAG AGCCCATCGC TGCGTCCACC AACAGAGGCA AAGATTTGAT TGGAGTCCAA
    AATCTGCTGA AGAAACACCA AGCCCTACAA GCAGAAATTG CTGGGCATGA ACCCCGCATC
    AAGGCGGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC CGCTGAGGAT
    GTGAAGGCTA AGCTCAGCGA ACTGAACCAG AAGTGGGAGT CCCTCAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA AGACTCCTTG CAGGCCCAGC AGTACTTTGC TGACGCCAAC
    GAGGCAGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACTGA CTACGGGAAG
    GACGAGGACT CCGCTGAGGC TCTACTGAAG AAACATGAAG CTTTGATGTC AGATCTCAGC
    GCCTACGGCA GCAGCATCCA GGCCCTGCGG GACCAGGCTC AGTCCTGCCG GCAACAAGTG
    GCCCCCATGG ATGACGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG
    AAGAGCCCTC GAGAGGTTAC CATGAAGAAA GGGGACATTC TCACTTTACT CAACAGCACC
    AACAAGGACT GGTGGAAGGT GGAAGTGAAC GACCGTCAGG GTTTCGTGCC AGCCGCGTAT
    GTGAAGAAGC TGGACCCTGC CCAGTCTGCT TCCCGAGAGA ACCTTCTGGA GGAGCAGGGC
    AGCATTGCTG TGCGGCAGGA GCAGATTGAC CACCAGACGC GCATAACTAA GGAGGCCGGC
    AGTGTGTCTC TGCGTATGAA ACAGGTGGAG GAACTGTATC ACTCTCTGCT GGAGCTGGGC
    GAGAAGCGCA AGGGCATGTT GGAGAAGAGC TGTAAGCGGT TCATGTTGTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAACGAGAAG GAGGCAGCTC TGACCAGCGA GGAGGTGGGC
    GCTGACCTGG AGCAGGTCGA AGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG
    AAGGCCAATG AGTCACGGCT GAAAGACATT AACAAGGTGG CTGAAGACCT GGAGTCTGAA
    GGTCTGATGG CTGAGGAGGT GCAGGCAGTA CAGCAGCAGG AGGTGTATGG CATGATGCCC
    AGGGATGACA CGGATTCCAA GACTGCATCC CCTTGGAAGT CTGCTCGTCT GATGGTTCAT
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GTTGGCGGTC CCTGCAGCAG
    CTGGCCGAGG AGCGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA
    GATGCCGATG AAACCAAAGA GTGGATTGAG GAGAAGAATC AGGCCCTAAA CACAGACAAC
    TATGGCCACG ATCTGGCCAG CGTACAAGCT CTGCAGCGCA AGCACGAGGG CTTTGAGAGG
    GACCTCGCGG CTCTTGGTGA CAAGGTCAAC TCCCTGGGGG AAACAGCCGA GCGCCTGATC
    CAGTCCCACC CGGAGTCGGC CGAGGACCTG CAGGAAAAGT GCACGGAGTT GGACCAGGCG
    TGGAGCAGCC TGGGGAAGCG CGCAGACCAG CGCAAGGCCA AGCTGGGTGA TTCCCACGAC
    CTGCAACGCT TCCTTAGTGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG
    TTGGTGTCCT CAGACGAGTT AGCCAAGGAT GTCACCGGAG CAGAAGCTTT GCTGGAACGG
    CACCAGGAGC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC CTTTGAGCAG
    TTTGGGCAGC AGCTGCTGGC TCATGGGCAC TACGCCAGCC CAGAGGTCAA AGAGAAACTA
    GATATTCTAG ATCGGGAGCG TGCGGGTCTG GAGAAGGCCT GGGCCCAGCG TAGGATGATG
    TTGGATCAGT GCCTTGAACT GCAGCTGTTT CATCGGGACT GTGAACAAGC TGAGAACTGG
    ATGGCCGCCC GGGAGGCCTT TCTGAATACT GAGGACAAAG GAGACTCACT GGACAGCGTG
    GAGGCTCTGA TCAAAAAACA CGAGGATTTT GACAAAGCGA TCAATGTCCA GGAGGAGAAG
    ATTGCCGCCT TGCAGTCCTT TGCTGACCAA CTTATTGCTG CCGGCCACTA TGCCAAGGGT
    GACATCTGCA GTCGGCGCAA TGAGGTTTTG GACAGGTGGC GACGCCTAAA AGCTCAGATG
    ATTGAGAAGA GATCGAAGCT GGGAGAATCA CAAACCCTCC AGCAGTTCAG CCGGGACGTG
    GATGAGATCG AGGCTTGGAT CAGTGAGAAA TTACAGACTG CGAGTGATGA GTCGTACAAA
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAAC ACCAGAAGCA CCAGGCCTTT
    GAGGCGGAAC TGCATGCCAA TGCTGACCGG ATCCGCGGGG TCATCGACAC GGGCAACTCC
    CTCATCGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG GCTGGCTGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAA AAGTCAGCCG AAAAGAGCCA GAAACTAAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGAATCAAGG ACTTTGACTT CTGGCTTTCT
    GAGGTGGAGG CCCTTCTGGC ATCTGAGGAC TACGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTGAAGA AACATCAACT GCTAGAGGCG GACATATCTG CCCACGAGGA TCGCCTGAAG
    GACCTGAATA GCCAAGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTG
    AAGGATAAGC GGGACACCAT CAATGGTCGT TTCCAGAAGA TCAAGAACAT GGCGGCCTCC
    CGGCGAGCCA GGCTGAATGA ATCCCATCGC CTACACCAGT TCTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA GAAAAAGTTG CTGGTGAGCT CAGAGGACTA TGGCCGAGAC
    CTTACAGGAG TTCAGAACCT GAGGAAGAAA CACAAGCGGC TGGAGGCGGA ACTGGCTGCC
    CATGAGCCAG CGATCCAGGG TGTCCTGGAC ACTGGGAAGA AGCTGTCTGA TGACAATACC
    ATTGGCAAGG AGGAGATTCA GCAGCGTCTG GCCCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CGGCAGCTCG GGGTCAGCGG CTAGAGGAGT CCTTGGAGTA TCAGCAGTTT
    GTAGCCAATG TGGAAGAAGA AGAAGCCTGG ATTAATGAGA AGATGACCCT GGTAGCCAGT
    GAGGATTATG GAGACACGCT TGCCGCTATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT
    GAGACAGACT TCACTGTGCA CAAGGATCGT GTGAACGACG TCTGCAGTAA TGGACAAGAC
    CTCATCAAGA AGAACAATCA CCACGAGGAG AACATCTCTT CAAAGATGAA GGGCTTGAAT
    GGCAAAGTGT CAGACTTGGA GAAAGCTGCT GCCCAGAGGA AGGCCAAGCT TGAGGAGAAC
    TCGGCCTTCC TTCAGTTCAA CTGGAAGGCT GATGTAGTGG AATCCTGGAT CGGTGAGAAG
    GAAAACAGCC TGAAGACAGA CGATTATGGC CGAGACCTGT CCTCCGTGCA GACTCTCCTC
    ACCAAACAGG AAACTTTTGA CGCTGGTCTA CAGGCCTTCC AGCAGGAGGG CATTGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACA TTCAGTCAAA GGCCATTGAA
    GCCCGGCACG CCTCTCTCAT GAAGAGATGG AGCCAGCTTC TGGCCAACTC GGCCACCCGC
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAAGACCT CTTCCTGACC
    TTCGCCAAAA AGGCATCGGC CTTCAACAGC TGGTTTGAAA ACGCCGAAGA GGATCTGACT
    GATCCTGTGC GTTGCAACTC CCTGGAGGAG ATCAGAGCTC TGCGTGAGGC CCACGACGCC
    TTCCGCTCAT CCCTCAGCTC TGCCCAGGCC GACTTCAGCC AGCTGGCTGA GCTGGACCGC
    CAGATCAAGA GCTTCCGTGT GGCCTCTAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACGT GGAGGAACCT GCAGAAGATC ATCAAGGAGC GGGAGCTGGA GCTGCAAAAG
    GAGCAGCGGC GACAGGAGGA GAATGACAAG CTACGCCAGG AGTTCGCCCA GCACGCTAAC
    GCCTTCCACC AGTGGATACA GGAAACCAGA ACATATCTCC TCGATGGCAT AGCGTATCGT
    CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG
    GTGGAAGAAT CCGGGACCCT GGAATCCCAG CTTGAGGCTA CCAAACGCAA GCACCAGGAG
    ATTCGAGCCA TGAGAAGTCA GCTCAAGAAG ATTGAGGACC TGGGGGCTGC CATGGAGGAG
    GCCCTGATCC TGGATAACAA GTATACAGAG CACAGCACCG TGGGCCTGGC CCAACAGTGG
    GACCAGCTCG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC
    AGGAACACAA CAGGAGTGAC GGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC
    TTCGACAAGG ACAAGTCAGG CCGGCTGAAC CACCAGGAGT TCAAGTCCTG CCTGCGCTCC
    CTGGGTTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCTG ACCCCGAGTT CGAGGCAATC
    CTAGACACCG TGGATCCAAA CAGGGATGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTC
    ATGATCAGCC GTGAAACGGA GAACGTTAAA TCCAGTGAGG AGATTGAGAG CGCCTTCCGG
    GCCCTGAGCT CCGAGGGAAA GCCCTACGTG ACCAAGGAGG AGCTGTATCA GAACCTGACT
    CGAGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGATGG CAAGGGCCGC
    GAACTCCCCA CTGCCTTCGA CTACGTGGAG TTCACCCGCT CGCTCTTTGT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_537823.4
    CDS152..7648
    Translation

    Target ORF information:

    RefSeq Version XM_537823.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_537823.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGACCCAA GTGGGGTCAA AGTGCTGGAA ACAGCAGAGG ACATCCAGGA GAGGCGGCAG 
    CAGGTCCTAG ACAGATACCA CCGCTTCAAG GAGCTCTCAA CCCTTAGGCG CCAGAAGCTT
    GAAGATTCCT ATCGATTCCA GTTTTTCCAG AGAGATGCTG AGGAGCTGGA GAAATGGATT
    CAGGAGAAAC TTCAGATCGC GTCAGATGAG AATTATAAAG ACCCAACCAA CTTGCAGGGC
    AAGCTTCAGA AGCATCAGGC CTTCGAAGCT GAAGTGCAGG CCAACTCAGG AGCCATTGTT
    AAACTGGATG AAACTGGAAA CCTGATGATC TCAGAAGGGC ATTTTGCGTC TGAAACCATA
    AGGACCCGTT TAATGGAGCT GCACCGCCAG TGGGAGCTGC TTTTGGAGAA GATGCGAGAG
    AAGGGAGTCA AACTGCTGCA GGCCCAGAAG CTGGTGCAGT ATTTGCGAGA GTGTGAGGAT
    GTGATGGACT GGATCAACGA CAAGGAAGCA ATTGTGACTT CTGAGGAGCT GGGCCAAGAC
    CTGGAGCATG TAGAGGTTTT ACAGAAGAAA TTTGAAGAGT TTCAAACAGA TATGGCTGCC
    CATGAAGAAA GAGTTAATGA AGTTAATCAG TTTGCTGCCA AGCTCATTCA GGAGCAGCAC
    CCCGAGGAGG AGACCATCAA GACCAAGCAA GATGAAGTAA ATGCGGCCTG GCAGCGGCTG
    AAGGGACTGG CCCTTCAGAG ACAGGGGAAG CTCTTCGGAG CTGCTGAGGT TCAGCGCTTT
    AACAGGGATG TGGATGAAAC CATCAGCTGG ATTAAAGAAA AGGAGCAATT AATGGCCTCT
    GACGATTTTG GCCGAGACCT GGCAAGTGTT CAGGCTCTGC TGCGGAAGCA CGAAGGCTTG
    GAGAGAGATC TTGCTGCTTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCCGACCGC
    CTGCAGCAGT CCCACCCTCT GAGTGCTGCA CAGATTCAAG TGAAGCGAGA GGAGCTGATT
    GCCAACTGGG AGCAGATCCG GACTTTGGCA GCTGAGAGAC ATGCCCGCCT CAATGACTCC
    TACAGGCTTC AACGCTTCCT TGCTGACTTC CGTGACCTTA CCAGCTGGGT AACTGAGATG
    AAAGCCCTTA TCAATGCAGA TGAGCTTGCC AATGATGTGG CCGGCGCCGA AGCCTTGCTG
    GACAGGCATC AAGAACACAA GGGTGAGATT GATGCTCACG AGGACAGCTT TAAATCTGCA
    GATGAGTCTG GACAGGCCCT GCTTGCTGCT GGGCATTATG CCTCAGATGA AGTGAGGGAG
    AAGCTGAGCA TCCTGGCTGA GGAGAGGGCC GCCCTGCTGG AGCTGTGGGA GCTGCGCCGG
    CAGCAGTATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCCTTCCTG CTGAATGAGG ACTTGGGAGA CTCTCTGGAT
    AGCGTGGAGG CGCTCCTCAA GAAGCATGAG GACTTTGAGA AATCCCTCAG CGCCCAGGAG
    GAAAAAATTA CAGCACTGGA TGAATTTGCA ACCAAGCTGA TTCAGAACAA CCACTACGCC
    ATGGAAGACG TGGCTACCCG CCGAGATGCG CTCCTGAGCC GCCGCAACGC TCTTCATGAA
    AGAGCCATGT GTCGTCGCGC CCAACTGGCA GACTCTTTCC ACCTGCAGCA ATTTTTCCGT
    GACTCTGATG AGCTCAAGAG CTGGGTCAAT GAGAAGATGA AAACCGCCAC AGATGAAGCT
    TATAAAGACC CATCCAACCT ACAAGGAAAA GTACAGAAGC ATCAGGCTTT TGAGGCTGAG
    CTCTCGGCCA ACCAGAGCCG CATTGACGCC CTGGAGAAAG CAGGGCAAAA GCTGATTGAT
    GTCAACCACT ATGCCAAAGA TGAGGTAGCA GCTCGTATGA ACGAGGTCAT CAGTTTGTGG
    AAGAAATTGC TTGAAGCCAC TGAACTGAAG GGGATAAAGC TTCGTGAGGC GAACCAGCAG
    CAGCAGTTCA ACCGCAATGT TGAGGATATT GAGCTATGGC TGTATGAAGT GGAAGGTCAC
    TTGGCTTCAG ACGATTATGG CAAAGACCTT ACCAATGTCC AGAACCTCCA GAAGAAGCAT
    GCCCTGCTAG AGGCCGATGT GGCTGCTCAC CAGGATCGAA TCGATGGCAT CACCATTCAG
    GCCCGCCAGT TCCAGGATGC TGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAA
    GCCCTTGTGG CTCGCTATGA GGCTCTCAAA GAACCCATGA TTGCCCGAAA GCAGAAGCTG
    GCTGATTCTC TACGCTTGCA GCAGCTCTTC CGTGATGTTG AGGATGAGGA GACGTGGATT
    AGGGAGAAAG AGCCCATCGC TGCGTCCACC AACAGAGGCA AAGATTTGAT TGGAGTCCAA
    AATCTGCTGA AGAAACACCA AGCCCTACAA GCAGAAATTG CTGGGCATGA ACCCCGCATC
    AAGGCGGTTA CACAGAAGGG GAATGCCATG GTGGAGGAAG GCCATTTTGC CGCTGAGGAT
    GTGAAGGCTA AGCTCAGCGA ACTGAACCAG AAGTGGGAGT CCCTCAAGTC CAAGGCCTCC
    CAGCGGCGGC AGGACCTGGA AGACTCCTTG CAGGCCCAGC AGTACTTTGC TGACGCCAAC
    GAGGCAGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACTGA CTACGGGAAG
    GACGAGGACT CCGCTGAGGC TCTACTGAAG AAACATGAAG CTTTGATGTC AGATCTCAGC
    GCCTACGGCA GCAGCATCCA GGCCCTGCGG GACCAGGCTC AGTCCTGCCG GCAACAAGTG
    GCCCCCATGG ATGACGAGAC TGGGAAGGAG CTGGTCTTGG CTCTCTACGA CTATCAGGAG
    AAGAGCCCTC GAGAGGTTAC CATGAAGAAA GGGGACATTC TCACTTTACT CAACAGCACC
    AACAAGGACT GGTGGAAGGT GGAAGTGAAC GACCGTCAGG GTTTCGTGCC AGCCGCGTAT
    GTGAAGAAGC TGGACCCTGC CCAGTCTGCT TCCCGAGAGA ACCTTCTGGA GGAGCAGGGC
    AGCATTGCTG TGCGGCAGGA GCAGATTGAC CACCAGACGC GCATAACTAA GGAGGCCGGC
    AGTGTGTCTC TGCGTATGAA ACAGGTGGAG GAACTGTATC ACTCTCTGCT GGAGCTGGGC
    GAGAAGCGCA AGGGCATGTT GGAGAAGAGC TGTAAGCGGT TCATGTTGTT CCGTGAGGCG
    AACGAGCTGC AGCAGTGGAT CAACGAGAAG GAGGCAGCTC TGACCAGCGA GGAGGTGGGC
    GCTGACCTGG AGCAGGTCGA AGTGCTGCAG AAGAAGTTCG ATGACTTCCA GAAGGATCTG
    AAGGCCAATG AGTCACGGCT GAAAGACATT AACAAGGTGG CTGAAGACCT GGAGTCTGAA
    GGTCTGATGG CTGAGGAGGT GCAGGCAGTA CAGCAGCAGG AGGTGTATGG CATGATGCCC
    AGGGATGACA CGGATTCCAA GACTGCATCC CCTTGGAAGT CTGCTCGTCT GATGGTTCAT
    ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTGAACGAGC GTTGGCGGTC CCTGCAGCAG
    CTGGCCGAGG AGCGGAGCCA GCTCTTGGGC AGCGCCCATG AAGTACAGAG GTTCCACAGA
    GATGCCGATG AAACCAAAGA GTGGATTGAG GAGAAGAATC AGGCCCTAAA CACAGACAAC
    TATGGCCACG ATCTGGCCAG CGTACAAGCT CTGCAGCGCA AGCACGAGGG CTTTGAGAGG
    GACCTCGCGG CTCTTGGTGA CAAGGTCAAC TCCCTGGGGG AAACAGCCGA GCGCCTGATC
    CAGTCCCACC CGGAGTCGGC CGAGGACCTG CAGGAAAAGT GCACGGAGTT GGACCAGGCG
    TGGAGCAGCC TGGGGAAGCG CGCAGACCAG CGCAAGGCCA AGCTGGGTGA TTCCCACGAC
    CTGCAACGCT TCCTTAGTGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG
    TTGGTGTCCT CAGACGAGTT AGCCAAGGAT GTCACCGGAG CAGAAGCTTT GCTGGAACGG
    CACCAGGAGC ACCGGACAGA AATCGATGCC AGGGCTGGCA CTTTCCAGGC CTTTGAGCAG
    TTTGGGCAGC AGCTGCTGGC TCATGGGCAC TACGCCAGCC CAGAGGTCAA AGAGAAACTA
    GATATTCTAG ATCGGGAGCG TGCGGGTCTG GAGAAGGCCT GGGCCCAGCG TAGGATGATG
    TTGGATCAGT GCCTTGAACT GCAGCTGTTT CATCGGGACT GTGAACAAGC TGAGAACTGG
    ATGGCCGCCC GGGAGGCCTT TCTGAATACT GAGGACAAAG GAGACTCACT GGACAGCGTG
    GAGGCTCTGA TCAAAAAACA CGAGGATTTT GACAAAGCGA TCAATGTCCA GGAGGAGAAG
    ATTGCCGCCT TGCAGTCCTT TGCTGACCAA CTTATTGCTG CCGGCCACTA TGCCAAGGGT
    GACATCTGCA GTCGGCGCAA TGAGGTTTTG GACAGGTGGC GACGCCTAAA AGCTCAGATG
    ATTGAGAAGA GATCGAAGCT GGGAGAATCA CAAACCCTCC AGCAGTTCAG CCGGGACGTG
    GATGAGATCG AGGCTTGGAT CAGTGAGAAA TTACAGACTG CGAGTGATGA GTCGTACAAA
    GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAAC ACCAGAAGCA CCAGGCCTTT
    GAGGCGGAAC TGCATGCCAA TGCTGACCGG ATCCGCGGGG TCATCGACAC GGGCAACTCC
    CTCATCGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG GCTGGCTGCC
    TTAGCTGACC AGTGGCAGTT CCTGGTGCAA AAGTCAGCCG AAAAGAGCCA GAAACTAAAA
    GAAGCCAACA AGCAGCAGAA CTTCAACACT GGAATCAAGG ACTTTGACTT CTGGCTTTCT
    GAGGTGGAGG CCCTTCTGGC ATCTGAGGAC TACGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTGAAGA AACATCAACT GCTAGAGGCG GACATATCTG CCCACGAGGA TCGCCTGAAG
    GACCTGAATA GCCAAGCAGA CAGCCTGATG ACCAGCAGTG CCTTCGACAC CTCCCAAGTG
    AAGGATAAGC GGGACACCAT CAATGGTCGT TTCCAGAAGA TCAAGAACAT GGCGGCCTCC
    CGGCGAGCCA GGCTGAATGA ATCCCATCGC CTACACCAGT TCTTCCGGGA CATGGATGAT
    GAGGAGTCCT GGATCAAGGA GAAAAAGTTG CTGGTGAGCT CAGAGGACTA TGGCCGAGAC
    CTTACAGGAG TTCAGAACCT GAGGAAGAAA CACAAGCGGC TGGAGGCGGA ACTGGCTGCC
    CATGAGCCAG CGATCCAGGG TGTCCTGGAC ACTGGGAAGA AGCTGTCTGA TGACAATACC
    ATTGGCAAGG AGGAGATTCA GCAGCGTCTG GCCCAGTTCG TGGAGCACTG GAAGGAGCTG
    AAGCAGCTGG CGGCAGCTCG GGGTCAGCGG CTAGAGGAGT CCTTGGAGTA TCAGCAGTTT
    GTAGCCAATG TGGAAGAAGA AGAAGCCTGG ATTAATGAGA AGATGACCCT GGTAGCCAGT
    GAGGATTATG GAGACACGCT TGCCGCTATC CAGGGCTTAC TGAAGAAACA TGAAGCTTTT
    GAGACAGACT TCACTGTGCA CAAGGATCGT GTGAACGACG TCTGCAGTAA TGGACAAGAC
    CTCATCAAGA AGAACAATCA CCACGAGGAG AACATCTCTT CAAAGATGAA GGGCTTGAAT
    GGCAAAGTGT CAGACTTGGA GAAAGCTGCT GCCCAGAGGA AGGCCAAGCT TGAGGAGAAC
    TCGGCCTTCC TTCAGTTCAA CTGGAAGGCT GATGTAGTGG AATCCTGGAT CGGTGAGAAG
    GAAAACAGCC TGAAGACAGA CGATTATGGC CGAGACCTGT CCTCCGTGCA GACTCTCCTC
    ACCAAACAGG AAACTTTTGA CGCTGGTCTA CAGGCCTTCC AGCAGGAGGG CATTGCCAAC
    ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCACA TTCAGTCAAA GGCCATTGAA
    GCCCGGCACG CCTCTCTCAT GAAGAGATGG AGCCAGCTTC TGGCCAACTC GGCCACCCGC
    AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAAGACCT CTTCCTGACC
    TTCGCCAAAA AGGCATCGGC CTTCAACAGC TGGTTTGAAA ACGCCGAAGA GGATCTGACT
    GATCCTGTGC GTTGCAACTC CCTGGAGGAG ATCAGAGCTC TGCGTGAGGC CCACGACGCC
    TTCCGCTCAT CCCTCAGCTC TGCCCAGGCC GACTTCAGCC AGCTGGCTGA GCTGGACCGC
    CAGATCAAGA GCTTCCGTGT GGCCTCTAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG
    GAGGAGACGT GGAGGAACCT GCAGAAGATC ATCAAGGAGC GGGAGCTGGA GCTGCAAAAG
    GAGCAGCGGC GACAGGAGGA GAATGACAAG CTACGCCAGG AGTTCGCCCA GCACGCTAAC
    GCCTTCCACC AGTGGATACA GGAAACCAGA ACATATCTCC TCGATGGCAT AGCGTATCGT
    CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG
    GTGGAAGAAT CCGGGACCCT GGAATCCCAG CTTGAGGCTA CCAAACGCAA GCACCAGGAG
    ATTCGAGCCA TGAGAAGTCA GCTCAAGAAG ATTGAGGACC TGGGGGCTGC CATGGAGGAG
    GCCCTGATCC TGGATAACAA GTATACAGAG CACAGCACCG TGGGCCTGGC CCAACAGTGG
    GACCAGCTCG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC
    AGGAACACAA CAGGAGTGAC GGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC
    TTCGACAAGG ACAAGTCAGG CCGGCTGAAC CACCAGGAGT TCAAGTCCTG CCTGCGCTCC
    CTGGGTTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCTG ACCCCGAGTT CGAGGCAATC
    CTAGACACCG TGGATCCAAA CAGGGATGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTC
    ATGATCAGCC GTGAAACGGA GAACGTTAAA TCCAGTGAGG AGATTGAGAG CGCCTTCCGG
    GCCCTGAGCT CCGAGGGAAA GCCCTACGTG ACCAAGGAGG AGCTGTATCA GAACCTGACT
    CGAGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGATGG CAAGGGCCGC
    GAACTCCCCA CTGCCTTCGA CTACGTGGAG TTCACCCGCT CGCTCTTTGT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12757
    Clone ID Related Accession (Same CDS sequence) XM_005625283.2 , XM_005625283.3
    Accession Version XM_005625283.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTGGAA
    TCCCAGCTTG AGGCTACCAA ACGCAAGCAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC
    AAGAAGATTG AGGACCTGGG GGCTGCCATG GAGGAGGCCC TGATCCTGGA TAACAAGTAT
    ACAGAGCACA GCACCGTGGG CCTGGCCCAA CAGTGGGACC AGCTCGACCA GCTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG AGTGACGGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCAGGCCGG
    CTGAACCACC AGGAGTTCAA GTCCTGCCTG CGCTCCCTGG GTTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCTGACCC CGAGTTCGAG GCAATCCTAG ACACCGTGGA TCCAAACAGG
    GATGGCCACG TCTCCCTGCA AGAGTACATG GCCTTCATGA TCAGCCGTGA AACGGAGAAC
    GTTAAATCCA GTGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA GGGAAAGCCC
    TACGTGACCA AGGAGGAGCT GTATCAGAAC CTGACTCGAG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGATGGCAAG GGCCGCGAAC TCCCCACTGC CTTCGACTAC
    GTGGAGTTCA CCCGCTCGCT CTTTGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625340.1
    CDS119..7591
    Translation

    Target ORF information:

    RefSeq Version XM_005625283.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625283.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTGGAA
    TCCCAGCTTG AGGCTACCAA ACGCAAGCAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC
    AAGAAGATTG AGGACCTGGG GGCTGCCATG GAGGAGGCCC TGATCCTGGA TAACAAGTAT
    ACAGAGCACA GCACCGTGGG CCTGGCCCAA CAGTGGGACC AGCTCGACCA GCTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG AGTGACGGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCAGGCCGG
    CTGAACCACC AGGAGTTCAA GTCCTGCCTG CGCTCCCTGG GTTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCTGACCC CGAGTTCGAG GCAATCCTAG ACACCGTGGA TCCAAACAGG
    GATGGCCACG TCTCCCTGCA AGAGTACATG GCCTTCATGA TCAGCCGTGA AACGGAGAAC
    GTTAAATCCA GTGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA GGGAAAGCCC
    TACGTGACCA AGGAGGAGCT GTATCAGAAC CTGACTCGAG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGATGGCAAG GGCCGCGAAC TCCCCACTGC CTTCGACTAC
    GTGGAGTTCA CCCGCTCGCT CTTTGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12757
    Clone ID Related Accession (Same CDS sequence) XM_005625283.2 , XM_005625283.3
    Accession Version XM_005625283.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625283.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTGGAA
    TCCCAGCTTG AGGCTACCAA ACGCAAGCAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC
    AAGAAGATTG AGGACCTGGG GGCTGCCATG GAGGAGGCCC TGATCCTGGA TAACAAGTAT
    ACAGAGCACA GCACCGTGGG CCTGGCCCAA CAGTGGGACC AGCTCGACCA GCTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG AGTGACGGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCAGGCCGG
    CTGAACCACC AGGAGTTCAA GTCCTGCCTG CGCTCCCTGG GTTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCTGACCC CGAGTTCGAG GCAATCCTAG ACACCGTGGA TCCAAACAGG
    GATGGCCACG TCTCCCTGCA AGAGTACATG GCCTTCATGA TCAGCCGTGA AACGGAGAAC
    GTTAAATCCA GTGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA GGGAAAGCCC
    TACGTGACCA AGGAGGAGCT GTATCAGAAC CTGACTCGAG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGATGGCAAG GGCCGCGAAC TCCCCACTGC CTTCGACTAC
    GTGGAGTTCA CCCGCTCGCT CTTTGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625340.1
    CDS137..7609
    Translation

    Target ORF information:

    RefSeq Version XM_005625283.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625283.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCCGG GACCCTGGAA
    TCCCAGCTTG AGGCTACCAA ACGCAAGCAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC
    AAGAAGATTG AGGACCTGGG GGCTGCCATG GAGGAGGCCC TGATCCTGGA TAACAAGTAT
    ACAGAGCACA GCACCGTGGG CCTGGCCCAA CAGTGGGACC AGCTCGACCA GCTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG AGTGACGGAG
    GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCAGGCCGG
    CTGAACCACC AGGAGTTCAA GTCCTGCCTG CGCTCCCTGG GTTATGACCT GCCCATGGTG
    GAGGAAGGGG AGCCTGACCC CGAGTTCGAG GCAATCCTAG ACACCGTGGA TCCAAACAGG
    GATGGCCACG TCTCCCTGCA AGAGTACATG GCCTTCATGA TCAGCCGTGA AACGGAGAAC
    GTTAAATCCA GTGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA GGGAAAGCCC
    TACGTGACCA AGGAGGAGCT GTATCAGAAC CTGACTCGAG AACAAGCCGA CTACTGCGTC
    TCCCACATGA AGCCCTACGT GGATGGCAAG GGCCGCGAAC TCCCCACTGC CTTCGACTAC
    GTGGAGTTCA CCCGCTCGCT CTTTGTGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12758
    Clone ID Related Accession (Same CDS sequence) XM_005625284.3 , XM_005625284.2
    Accession Version XM_005625284.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625284.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCCGGGAC CCTGGAATCC
    CAGCTTGAGG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG
    AAGATTGAGG ACCTGGGGGC TGCCATGGAG GAGGCCCTGA TCCTGGATAA CAAGTATACA
    GAGCACAGCA CCGTGGGCCT GGCCCAACAG TGGGACCAGC TCGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACGGAGGAG
    GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC AGGCCGGCTG
    AACCACCAGG AGTTCAAGTC CTGCCTGCGC TCCCTGGGTT ATGACCTGCC CATGGTGGAG
    GAAGGGGAGC CTGACCCCGA GTTCGAGGCA ATCCTAGACA CCGTGGATCC AAACAGGGAT
    GGCCACGTCT CCCTGCAAGA GTACATGGCC TTCATGATCA GCCGTGAAAC GGAGAACGTT
    AAATCCAGTG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAGGG AAAGCCCTAC
    GTGACCAAGG AGGAGCTGTA TCAGAACCTG ACTCGAGAAC AAGCCGACTA CTGCGTCTCC
    CACATGAAGC CCTACGTGGA TGGCAAGGGC CGCGAACTCC CCACTGCCTT CGACTACGTG
    GAGTTCACCC GCTCGCTCTT TGTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625341.1
    CDS137..7606
    Translation

    Target ORF information:

    RefSeq Version XM_005625284.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625284.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCCGGGAC CCTGGAATCC
    CAGCTTGAGG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG
    AAGATTGAGG ACCTGGGGGC TGCCATGGAG GAGGCCCTGA TCCTGGATAA CAAGTATACA
    GAGCACAGCA CCGTGGGCCT GGCCCAACAG TGGGACCAGC TCGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACGGAGGAG
    GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC AGGCCGGCTG
    AACCACCAGG AGTTCAAGTC CTGCCTGCGC TCCCTGGGTT ATGACCTGCC CATGGTGGAG
    GAAGGGGAGC CTGACCCCGA GTTCGAGGCA ATCCTAGACA CCGTGGATCC AAACAGGGAT
    GGCCACGTCT CCCTGCAAGA GTACATGGCC TTCATGATCA GCCGTGAAAC GGAGAACGTT
    AAATCCAGTG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAGGG AAAGCCCTAC
    GTGACCAAGG AGGAGCTGTA TCAGAACCTG ACTCGAGAAC AAGCCGACTA CTGCGTCTCC
    CACATGAAGC CCTACGTGGA TGGCAAGGGC CGCGAACTCC CCACTGCCTT CGACTACGTG
    GAGTTCACCC GCTCGCTCTT TGTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12758
    Clone ID Related Accession (Same CDS sequence) XM_005625284.3 , XM_005625284.2
    Accession Version XM_005625284.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCCGGGAC CCTGGAATCC
    CAGCTTGAGG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG
    AAGATTGAGG ACCTGGGGGC TGCCATGGAG GAGGCCCTGA TCCTGGATAA CAAGTATACA
    GAGCACAGCA CCGTGGGCCT GGCCCAACAG TGGGACCAGC TCGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACGGAGGAG
    GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC AGGCCGGCTG
    AACCACCAGG AGTTCAAGTC CTGCCTGCGC TCCCTGGGTT ATGACCTGCC CATGGTGGAG
    GAAGGGGAGC CTGACCCCGA GTTCGAGGCA ATCCTAGACA CCGTGGATCC AAACAGGGAT
    GGCCACGTCT CCCTGCAAGA GTACATGGCC TTCATGATCA GCCGTGAAAC GGAGAACGTT
    AAATCCAGTG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAGGG AAAGCCCTAC
    GTGACCAAGG AGGAGCTGTA TCAGAACCTG ACTCGAGAAC AAGCCGACTA CTGCGTCTCC
    CACATGAAGC CCTACGTGGA TGGCAAGGGC CGCGAACTCC CCACTGCCTT CGACTACGTG
    GAGTTCACCC GCTCGCTCTT TGTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625341.1
    CDS119..7588
    Translation

    Target ORF information:

    RefSeq Version XM_005625284.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625284.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCCGGGAC CCTGGAATCC
    CAGCTTGAGG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG
    AAGATTGAGG ACCTGGGGGC TGCCATGGAG GAGGCCCTGA TCCTGGATAA CAAGTATACA
    GAGCACAGCA CCGTGGGCCT GGCCCAACAG TGGGACCAGC TCGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACGGAGGAG
    GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC AGGCCGGCTG
    AACCACCAGG AGTTCAAGTC CTGCCTGCGC TCCCTGGGTT ATGACCTGCC CATGGTGGAG
    GAAGGGGAGC CTGACCCCGA GTTCGAGGCA ATCCTAGACA CCGTGGATCC AAACAGGGAT
    GGCCACGTCT CCCTGCAAGA GTACATGGCC TTCATGATCA GCCGTGAAAC GGAGAACGTT
    AAATCCAGTG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAGGG AAAGCCCTAC
    GTGACCAAGG AGGAGCTGTA TCAGAACCTG ACTCGAGAAC AAGCCGACTA CTGCGTCTCC
    CACATGAAGC CCTACGTGGA TGGCAAGGGC CGCGAACTCC CCACTGCCTT CGACTACGTG
    GAGTTCACCC GCTCGCTCTT TGTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12759
    Clone ID Related Accession (Same CDS sequence) XM_005625285.2 , XM_005625285.3
    Accession Version XM_005625285.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGAGCAAA
    CACCAGAAGC ACCAGGCCTT TGAGGCGGAA CTGCATGCCA ATGCTGACCG GATCCGCGGG
    GTCATCGACA CGGGCAACTC CCTCATCGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GGCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA AAAGTCAGCC
    GAAAAGAGCC AGAAACTAAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGAATCAAG
    GACTTTGACT TCTGGCTTTC TGAGGTGGAG GCCCTTCTGG CATCTGAGGA CTACGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAG AAACATCAAC TGCTAGAGGC GGACATATCT
    GCCCACGAGG ATCGCCTGAA GGACCTGAAT AGCCAAGCAG ACAGCCTGAT GACCAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAGGATAAG CGGGACACCA TCAATGGTCG TTTCCAGAAG
    ATCAAGAACA TGGCGGCCTC CCGGCGAGCC AGGCTGAATG AATCCCATCG CCTACACCAG
    TTCTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AGAAAAAGTT GCTGGTGAGC
    TCAGAGGACT ATGGCCGAGA CCTTACAGGA GTTCAGAACC TGAGGAAGAA ACACAAGCGG
    CTGGAGGCGG AACTGGCTGC CCATGAGCCA GCGATCCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAATAC CATTGGCAAG GAGGAGATTC AGCAGCGTCT GGCCCAGTTC
    GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCGGCAGCTC GGGGTCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAT GTGGAAGAAG AAGAAGCCTG GATTAATGAG
    AAGATGACCC TGGTAGCCAG TGAGGATTAT GGAGACACGC TTGCCGCTAT CCAGGGCTTA
    CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACTGTGC ACAAGGATCG TGTGAACGAC
    GTCTGCAGTA ATGGACAAGA CCTCATCAAG AAGAACAATC ACCACGAGGA GAACATCTCT
    TCAAAGATGA AGGGCTTGAA TGGCAAAGTG TCAGACTTGG AGAAAGCTGC TGCCCAGAGG
    AAGGCCAAGC TTGAGGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC TGATGTAGTG
    GAATCCTGGA TCGGTGAGAA GGAAAACAGC CTGAAGACAG ACGATTATGG CCGAGACCTG
    TCCTCCGTGC AGACTCTCCT CACCAAACAG GAAACTTTTG ACGCTGGTCT ACAGGCCTTC
    CAGCAGGAGG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC
    ATTCAGTCAA AGGCCATTGA AGCCCGGCAC GCCTCTCTCA TGAAGAGATG GAGCCAGCTT
    CTGGCCAACT CGGCCACCCG CAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG
    GTGGAAGACC TCTTCCTGAC CTTCGCCAAA AAGGCATCGG CCTTCAACAG CTGGTTTGAA
    AACGCCGAAG AGGATCTGAC TGATCCTGTG CGTTGCAACT CCCTGGAGGA GATCAGAGCT
    CTGCGTGAGG CCCACGACGC CTTCCGCTCA TCCCTCAGCT CTGCCCAGGC CGACTTCAGC
    CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGTG TGGCCTCTAA CCCCTACACC
    TGGTTCACCA TGGAGGCCCT GGAGGAGACG TGGAGGAACC TGCAGAAGAT CATCAAGGAG
    CGGGAGCTGG AGCTGCAAAA GGAGCAGCGG CGACAGGAGG AGAATGACAA GCTACGCCAG
    GAGTTCGCCC AGCACGCTAA CGCCTTCCAC CAGTGGATAC AGGAAACCAG AACATATCTC
    CTCGATGGGT CCTGCATGGT GGAAGAATCC GGGACCCTGG AATCCCAGCT TGAGGCTACC
    AAACGCAAGC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAGGACCTG
    GGGGCTGCCA TGGAGGAGGC CCTGATCCTG GATAACAAGT ATACAGAGCA CAGCACCGTG
    GGCCTGGCCC AACAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG
    GAGCAGCAGA TCCAGGCCAG GAACACAACA GGAGTGACGG AGGAGGCCCT CAAAGAATTC
    AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCAGGCC GGCTGAACCA CCAGGAGTTC
    AAGTCCTGCC TGCGCTCCCT GGGTTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCTGAC
    CCCGAGTTCG AGGCAATCCT AGACACCGTG GATCCAAACA GGGATGGCCA CGTCTCCCTG
    CAAGAGTACA TGGCCTTCAT GATCAGCCGT GAAACGGAGA ACGTTAAATC CAGTGAGGAG
    ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAGGGAAAGC CCTACGTGAC CAAGGAGGAG
    CTGTATCAGA ACCTGACTCG AGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC
    GTGGATGGCA AGGGCCGCGA ACTCCCCACT GCCTTCGACT ACGTGGAGTT CACCCGCTCG
    CTCTTTGTGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625342.1
    CDS119..7573
    Translation

    Target ORF information:

    RefSeq Version XM_005625285.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625285.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGAGCAAA
    CACCAGAAGC ACCAGGCCTT TGAGGCGGAA CTGCATGCCA ATGCTGACCG GATCCGCGGG
    GTCATCGACA CGGGCAACTC CCTCATCGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GGCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA AAAGTCAGCC
    GAAAAGAGCC AGAAACTAAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGAATCAAG
    GACTTTGACT TCTGGCTTTC TGAGGTGGAG GCCCTTCTGG CATCTGAGGA CTACGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAG AAACATCAAC TGCTAGAGGC GGACATATCT
    GCCCACGAGG ATCGCCTGAA GGACCTGAAT AGCCAAGCAG ACAGCCTGAT GACCAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAGGATAAG CGGGACACCA TCAATGGTCG TTTCCAGAAG
    ATCAAGAACA TGGCGGCCTC CCGGCGAGCC AGGCTGAATG AATCCCATCG CCTACACCAG
    TTCTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AGAAAAAGTT GCTGGTGAGC
    TCAGAGGACT ATGGCCGAGA CCTTACAGGA GTTCAGAACC TGAGGAAGAA ACACAAGCGG
    CTGGAGGCGG AACTGGCTGC CCATGAGCCA GCGATCCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAATAC CATTGGCAAG GAGGAGATTC AGCAGCGTCT GGCCCAGTTC
    GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCGGCAGCTC GGGGTCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAT GTGGAAGAAG AAGAAGCCTG GATTAATGAG
    AAGATGACCC TGGTAGCCAG TGAGGATTAT GGAGACACGC TTGCCGCTAT CCAGGGCTTA
    CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACTGTGC ACAAGGATCG TGTGAACGAC
    GTCTGCAGTA ATGGACAAGA CCTCATCAAG AAGAACAATC ACCACGAGGA GAACATCTCT
    TCAAAGATGA AGGGCTTGAA TGGCAAAGTG TCAGACTTGG AGAAAGCTGC TGCCCAGAGG
    AAGGCCAAGC TTGAGGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC TGATGTAGTG
    GAATCCTGGA TCGGTGAGAA GGAAAACAGC CTGAAGACAG ACGATTATGG CCGAGACCTG
    TCCTCCGTGC AGACTCTCCT CACCAAACAG GAAACTTTTG ACGCTGGTCT ACAGGCCTTC
    CAGCAGGAGG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC
    ATTCAGTCAA AGGCCATTGA AGCCCGGCAC GCCTCTCTCA TGAAGAGATG GAGCCAGCTT
    CTGGCCAACT CGGCCACCCG CAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG
    GTGGAAGACC TCTTCCTGAC CTTCGCCAAA AAGGCATCGG CCTTCAACAG CTGGTTTGAA
    AACGCCGAAG AGGATCTGAC TGATCCTGTG CGTTGCAACT CCCTGGAGGA GATCAGAGCT
    CTGCGTGAGG CCCACGACGC CTTCCGCTCA TCCCTCAGCT CTGCCCAGGC CGACTTCAGC
    CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGTG TGGCCTCTAA CCCCTACACC
    TGGTTCACCA TGGAGGCCCT GGAGGAGACG TGGAGGAACC TGCAGAAGAT CATCAAGGAG
    CGGGAGCTGG AGCTGCAAAA GGAGCAGCGG CGACAGGAGG AGAATGACAA GCTACGCCAG
    GAGTTCGCCC AGCACGCTAA CGCCTTCCAC CAGTGGATAC AGGAAACCAG AACATATCTC
    CTCGATGGGT CCTGCATGGT GGAAGAATCC GGGACCCTGG AATCCCAGCT TGAGGCTACC
    AAACGCAAGC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAGGACCTG
    GGGGCTGCCA TGGAGGAGGC CCTGATCCTG GATAACAAGT ATACAGAGCA CAGCACCGTG
    GGCCTGGCCC AACAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG
    GAGCAGCAGA TCCAGGCCAG GAACACAACA GGAGTGACGG AGGAGGCCCT CAAAGAATTC
    AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCAGGCC GGCTGAACCA CCAGGAGTTC
    AAGTCCTGCC TGCGCTCCCT GGGTTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCTGAC
    CCCGAGTTCG AGGCAATCCT AGACACCGTG GATCCAAACA GGGATGGCCA CGTCTCCCTG
    CAAGAGTACA TGGCCTTCAT GATCAGCCGT GAAACGGAGA ACGTTAAATC CAGTGAGGAG
    ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAGGGAAAGC CCTACGTGAC CAAGGAGGAG
    CTGTATCAGA ACCTGACTCG AGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC
    GTGGATGGCA AGGGCCGCGA ACTCCCCACT GCCTTCGACT ACGTGGAGTT CACCCGCTCG
    CTCTTTGTGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12759
    Clone ID Related Accession (Same CDS sequence) XM_005625285.2 , XM_005625285.3
    Accession Version XM_005625285.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625285.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2521
    2581
    2641
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    3001
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    3181
    3241
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    4141
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    4441
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    4681
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    4921
    4981
    5041
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    5341
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    5641
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    5881
    5941
    6001
    6061
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    6181
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    6301
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    6421
    6481
    6541
    6601
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    6781
    6841
    6901
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    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGAGCAAA
    CACCAGAAGC ACCAGGCCTT TGAGGCGGAA CTGCATGCCA ATGCTGACCG GATCCGCGGG
    GTCATCGACA CGGGCAACTC CCTCATCGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GGCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA AAAGTCAGCC
    GAAAAGAGCC AGAAACTAAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGAATCAAG
    GACTTTGACT TCTGGCTTTC TGAGGTGGAG GCCCTTCTGG CATCTGAGGA CTACGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAG AAACATCAAC TGCTAGAGGC GGACATATCT
    GCCCACGAGG ATCGCCTGAA GGACCTGAAT AGCCAAGCAG ACAGCCTGAT GACCAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAGGATAAG CGGGACACCA TCAATGGTCG TTTCCAGAAG
    ATCAAGAACA TGGCGGCCTC CCGGCGAGCC AGGCTGAATG AATCCCATCG CCTACACCAG
    TTCTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AGAAAAAGTT GCTGGTGAGC
    TCAGAGGACT ATGGCCGAGA CCTTACAGGA GTTCAGAACC TGAGGAAGAA ACACAAGCGG
    CTGGAGGCGG AACTGGCTGC CCATGAGCCA GCGATCCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAATAC CATTGGCAAG GAGGAGATTC AGCAGCGTCT GGCCCAGTTC
    GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCGGCAGCTC GGGGTCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAT GTGGAAGAAG AAGAAGCCTG GATTAATGAG
    AAGATGACCC TGGTAGCCAG TGAGGATTAT GGAGACACGC TTGCCGCTAT CCAGGGCTTA
    CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACTGTGC ACAAGGATCG TGTGAACGAC
    GTCTGCAGTA ATGGACAAGA CCTCATCAAG AAGAACAATC ACCACGAGGA GAACATCTCT
    TCAAAGATGA AGGGCTTGAA TGGCAAAGTG TCAGACTTGG AGAAAGCTGC TGCCCAGAGG
    AAGGCCAAGC TTGAGGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC TGATGTAGTG
    GAATCCTGGA TCGGTGAGAA GGAAAACAGC CTGAAGACAG ACGATTATGG CCGAGACCTG
    TCCTCCGTGC AGACTCTCCT CACCAAACAG GAAACTTTTG ACGCTGGTCT ACAGGCCTTC
    CAGCAGGAGG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC
    ATTCAGTCAA AGGCCATTGA AGCCCGGCAC GCCTCTCTCA TGAAGAGATG GAGCCAGCTT
    CTGGCCAACT CGGCCACCCG CAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG
    GTGGAAGACC TCTTCCTGAC CTTCGCCAAA AAGGCATCGG CCTTCAACAG CTGGTTTGAA
    AACGCCGAAG AGGATCTGAC TGATCCTGTG CGTTGCAACT CCCTGGAGGA GATCAGAGCT
    CTGCGTGAGG CCCACGACGC CTTCCGCTCA TCCCTCAGCT CTGCCCAGGC CGACTTCAGC
    CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGTG TGGCCTCTAA CCCCTACACC
    TGGTTCACCA TGGAGGCCCT GGAGGAGACG TGGAGGAACC TGCAGAAGAT CATCAAGGAG
    CGGGAGCTGG AGCTGCAAAA GGAGCAGCGG CGACAGGAGG AGAATGACAA GCTACGCCAG
    GAGTTCGCCC AGCACGCTAA CGCCTTCCAC CAGTGGATAC AGGAAACCAG AACATATCTC
    CTCGATGGGT CCTGCATGGT GGAAGAATCC GGGACCCTGG AATCCCAGCT TGAGGCTACC
    AAACGCAAGC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAGGACCTG
    GGGGCTGCCA TGGAGGAGGC CCTGATCCTG GATAACAAGT ATACAGAGCA CAGCACCGTG
    GGCCTGGCCC AACAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG
    GAGCAGCAGA TCCAGGCCAG GAACACAACA GGAGTGACGG AGGAGGCCCT CAAAGAATTC
    AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCAGGCC GGCTGAACCA CCAGGAGTTC
    AAGTCCTGCC TGCGCTCCCT GGGTTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCTGAC
    CCCGAGTTCG AGGCAATCCT AGACACCGTG GATCCAAACA GGGATGGCCA CGTCTCCCTG
    CAAGAGTACA TGGCCTTCAT GATCAGCCGT GAAACGGAGA ACGTTAAATC CAGTGAGGAG
    ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAGGGAAAGC CCTACGTGAC CAAGGAGGAG
    CTGTATCAGA ACCTGACTCG AGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC
    GTGGATGGCA AGGGCCGCGA ACTCCCCACT GCCTTCGACT ACGTGGAGTT CACCCGCTCG
    CTCTTTGTGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625342.1
    CDS137..7591
    Translation

    Target ORF information:

    RefSeq Version XM_005625285.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625285.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGACGCGCAT AACTAAGGAG GCCGGCAGTG TGTCTCTGCG TATGAAACAG
    GTGGAGGAAC TGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGAGCAAA
    CACCAGAAGC ACCAGGCCTT TGAGGCGGAA CTGCATGCCA ATGCTGACCG GATCCGCGGG
    GTCATCGACA CGGGCAACTC CCTCATCGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GGCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA AAAGTCAGCC
    GAAAAGAGCC AGAAACTAAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGAATCAAG
    GACTTTGACT TCTGGCTTTC TGAGGTGGAG GCCCTTCTGG CATCTGAGGA CTACGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAG AAACATCAAC TGCTAGAGGC GGACATATCT
    GCCCACGAGG ATCGCCTGAA GGACCTGAAT AGCCAAGCAG ACAGCCTGAT GACCAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAGGATAAG CGGGACACCA TCAATGGTCG TTTCCAGAAG
    ATCAAGAACA TGGCGGCCTC CCGGCGAGCC AGGCTGAATG AATCCCATCG CCTACACCAG
    TTCTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AGAAAAAGTT GCTGGTGAGC
    TCAGAGGACT ATGGCCGAGA CCTTACAGGA GTTCAGAACC TGAGGAAGAA ACACAAGCGG
    CTGGAGGCGG AACTGGCTGC CCATGAGCCA GCGATCCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAATAC CATTGGCAAG GAGGAGATTC AGCAGCGTCT GGCCCAGTTC
    GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCGGCAGCTC GGGGTCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAT GTGGAAGAAG AAGAAGCCTG GATTAATGAG
    AAGATGACCC TGGTAGCCAG TGAGGATTAT GGAGACACGC TTGCCGCTAT CCAGGGCTTA
    CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACTGTGC ACAAGGATCG TGTGAACGAC
    GTCTGCAGTA ATGGACAAGA CCTCATCAAG AAGAACAATC ACCACGAGGA GAACATCTCT
    TCAAAGATGA AGGGCTTGAA TGGCAAAGTG TCAGACTTGG AGAAAGCTGC TGCCCAGAGG
    AAGGCCAAGC TTGAGGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC TGATGTAGTG
    GAATCCTGGA TCGGTGAGAA GGAAAACAGC CTGAAGACAG ACGATTATGG CCGAGACCTG
    TCCTCCGTGC AGACTCTCCT CACCAAACAG GAAACTTTTG ACGCTGGTCT ACAGGCCTTC
    CAGCAGGAGG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC
    ATTCAGTCAA AGGCCATTGA AGCCCGGCAC GCCTCTCTCA TGAAGAGATG GAGCCAGCTT
    CTGGCCAACT CGGCCACCCG CAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG
    GTGGAAGACC TCTTCCTGAC CTTCGCCAAA AAGGCATCGG CCTTCAACAG CTGGTTTGAA
    AACGCCGAAG AGGATCTGAC TGATCCTGTG CGTTGCAACT CCCTGGAGGA GATCAGAGCT
    CTGCGTGAGG CCCACGACGC CTTCCGCTCA TCCCTCAGCT CTGCCCAGGC CGACTTCAGC
    CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGTG TGGCCTCTAA CCCCTACACC
    TGGTTCACCA TGGAGGCCCT GGAGGAGACG TGGAGGAACC TGCAGAAGAT CATCAAGGAG
    CGGGAGCTGG AGCTGCAAAA GGAGCAGCGG CGACAGGAGG AGAATGACAA GCTACGCCAG
    GAGTTCGCCC AGCACGCTAA CGCCTTCCAC CAGTGGATAC AGGAAACCAG AACATATCTC
    CTCGATGGGT CCTGCATGGT GGAAGAATCC GGGACCCTGG AATCCCAGCT TGAGGCTACC
    AAACGCAAGC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAGGACCTG
    GGGGCTGCCA TGGAGGAGGC CCTGATCCTG GATAACAAGT ATACAGAGCA CAGCACCGTG
    GGCCTGGCCC AACAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG
    GAGCAGCAGA TCCAGGCCAG GAACACAACA GGAGTGACGG AGGAGGCCCT CAAAGAATTC
    AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCAGGCC GGCTGAACCA CCAGGAGTTC
    AAGTCCTGCC TGCGCTCCCT GGGTTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCTGAC
    CCCGAGTTCG AGGCAATCCT AGACACCGTG GATCCAAACA GGGATGGCCA CGTCTCCCTG
    CAAGAGTACA TGGCCTTCAT GATCAGCCGT GAAACGGAGA ACGTTAAATC CAGTGAGGAG
    ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAGGGAAAGC CCTACGTGAC CAAGGAGGAG
    CTGTATCAGA ACCTGACTCG AGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC
    GTGGATGGCA AGGGCCGCGA ACTCCCCACT GCCTTCGACT ACGTGGAGTT CACCCGCTCG
    CTCTTTGTGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12760
    Clone ID Related Accession (Same CDS sequence) XM_005625286.2 , XM_005625286.3
    Accession Version XM_005625286.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625286.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCCGGGAC CCTGGAATCC
    CAGCTTGAGG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG
    AAGATTGAGG ACCTGGGGGC TGCCATGGAG GAGGCCCTGA TCCTGGATAA CAAGTATACA
    GAGCACAGCA CCGTGGGCCT GGCCCAACAG TGGGACCAGC TCGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACGGAGGAG
    GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC AGGCCGGCTG
    AACCACCAGG AGTTCAAGTC CTGCCTGCGC TCCCTGGGTT ATGACCTGCC CATGGTGGAG
    GAAGGGGAGC CTGACCCCGA GTTCGAGGCA ATCCTAGACA CCGTGGATCC AAACAGGGAT
    GGCCACGTCT CCCTGCAAGA GTACATGGCC TTCATGATCA GCCGTGAAAC GGAGAACGTT
    AAATCCAGTG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAGGG AAAGCCCTAC
    GTGACCAAGG AGGAGCTGTA TCAGAACCTG ACTCGAGAAC AAGCCGACTA CTGCGTCTCC
    CACATGAAGC CCTACGTGGA TGGCAAGGGC CGCGAACTCC CCACTGCCTT CGACTACGTG
    GAGTTCACCC GCTCGCTCTT TGTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625343.1
    CDS119..7528
    Translation

    Target ORF information:

    RefSeq Version XM_005625286.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625286.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCCGGGAC CCTGGAATCC
    CAGCTTGAGG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG
    AAGATTGAGG ACCTGGGGGC TGCCATGGAG GAGGCCCTGA TCCTGGATAA CAAGTATACA
    GAGCACAGCA CCGTGGGCCT GGCCCAACAG TGGGACCAGC TCGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACGGAGGAG
    GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC AGGCCGGCTG
    AACCACCAGG AGTTCAAGTC CTGCCTGCGC TCCCTGGGTT ATGACCTGCC CATGGTGGAG
    GAAGGGGAGC CTGACCCCGA GTTCGAGGCA ATCCTAGACA CCGTGGATCC AAACAGGGAT
    GGCCACGTCT CCCTGCAAGA GTACATGGCC TTCATGATCA GCCGTGAAAC GGAGAACGTT
    AAATCCAGTG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAGGG AAAGCCCTAC
    GTGACCAAGG AGGAGCTGTA TCAGAACCTG ACTCGAGAAC AAGCCGACTA CTGCGTCTCC
    CACATGAAGC CCTACGTGGA TGGCAAGGGC CGCGAACTCC CCACTGCCTT CGACTACGTG
    GAGTTCACCC GCTCGCTCTT TGTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12760
    Clone ID Related Accession (Same CDS sequence) XM_005625286.2 , XM_005625286.3
    Accession Version XM_005625286.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625286.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCCGGGAC CCTGGAATCC
    CAGCTTGAGG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG
    AAGATTGAGG ACCTGGGGGC TGCCATGGAG GAGGCCCTGA TCCTGGATAA CAAGTATACA
    GAGCACAGCA CCGTGGGCCT GGCCCAACAG TGGGACCAGC TCGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACGGAGGAG
    GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC AGGCCGGCTG
    AACCACCAGG AGTTCAAGTC CTGCCTGCGC TCCCTGGGTT ATGACCTGCC CATGGTGGAG
    GAAGGGGAGC CTGACCCCGA GTTCGAGGCA ATCCTAGACA CCGTGGATCC AAACAGGGAT
    GGCCACGTCT CCCTGCAAGA GTACATGGCC TTCATGATCA GCCGTGAAAC GGAGAACGTT
    AAATCCAGTG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAGGG AAAGCCCTAC
    GTGACCAAGG AGGAGCTGTA TCAGAACCTG ACTCGAGAAC AAGCCGACTA CTGCGTCTCC
    CACATGAAGC CCTACGTGGA TGGCAAGGGC CGCGAACTCC CCACTGCCTT CGACTACGTG
    GAGTTCACCC GCTCGCTCTT TGTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625343.1
    CDS137..7546
    Translation

    Target ORF information:

    RefSeq Version XM_005625286.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625286.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGCTTTCC
    AAGCTGCTGA GCAAACACCA GAAGCACCAG GCCTTTGAGG CGGAACTGCA TGCCAATGCT
    GACCGGATCC GCGGGGTCAT CGACACGGGC AACTCCCTCA TCGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGGCTG GCTGCCTTAG CTGACCAGTG GCAGTTCCTG
    GTGCAAAAGT CAGCCGAAAA GAGCCAGAAA CTAAAAGAAG CCAACAAGCA GCAGAACTTC
    AACACTGGAA TCAAGGACTT TGACTTCTGG CTTTCTGAGG TGGAGGCCCT TCTGGCATCT
    GAGGACTACG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAGAAACA TCAACTGCTA
    GAGGCGGACA TATCTGCCCA CGAGGATCGC CTGAAGGACC TGAATAGCCA AGCAGACAGC
    CTGATGACCA GCAGTGCCTT CGACACCTCC CAAGTGAAGG ATAAGCGGGA CACCATCAAT
    GGTCGTTTCC AGAAGATCAA GAACATGGCG GCCTCCCGGC GAGCCAGGCT GAATGAATCC
    CATCGCCTAC ACCAGTTCTT CCGGGACATG GATGATGAGG AGTCCTGGAT CAAGGAGAAA
    AAGTTGCTGG TGAGCTCAGA GGACTATGGC CGAGACCTTA CAGGAGTTCA GAACCTGAGG
    AAGAAACACA AGCGGCTGGA GGCGGAACTG GCTGCCCATG AGCCAGCGAT CCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AATACCATTG GCAAGGAGGA GATTCAGCAG
    CGTCTGGCCC AGTTCGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCGGC AGCTCGGGGT
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAATGTGGA AGAAGAAGAA
    GCCTGGATTA ATGAGAAGAT GACCCTGGTA GCCAGTGAGG ATTATGGAGA CACGCTTGCC
    GCTATCCAGG GCTTACTGAA GAAACATGAA GCTTTTGAGA CAGACTTCAC TGTGCACAAG
    GATCGTGTGA ACGACGTCTG CAGTAATGGA CAAGACCTCA TCAAGAAGAA CAATCACCAC
    GAGGAGAACA TCTCTTCAAA GATGAAGGGC TTGAATGGCA AAGTGTCAGA CTTGGAGAAA
    GCTGCTGCCC AGAGGAAGGC CAAGCTTGAG GAGAACTCGG CCTTCCTTCA GTTCAACTGG
    AAGGCTGATG TAGTGGAATC CTGGATCGGT GAGAAGGAAA ACAGCCTGAA GACAGACGAT
    TATGGCCGAG ACCTGTCCTC CGTGCAGACT CTCCTCACCA AACAGGAAAC TTTTGACGCT
    GGTCTACAGG CCTTCCAGCA GGAGGGCATT GCCAACATCA CTGCCCTCAA AGATCAGCTG
    CTGGCCGCCA AGCACATTCA GTCAAAGGCC ATTGAAGCCC GGCACGCCTC TCTCATGAAG
    AGATGGAGCC AGCTTCTGGC CAACTCGGCC ACCCGCAAGA AGAAGCTGCT GGAGGCACAG
    AGTCACTTCC GCAAGGTGGA AGACCTCTTC CTGACCTTCG CCAAAAAGGC ATCGGCCTTC
    AACAGCTGGT TTGAAAACGC CGAAGAGGAT CTGACTGATC CTGTGCGTTG CAACTCCCTG
    GAGGAGATCA GAGCTCTGCG TGAGGCCCAC GACGCCTTCC GCTCATCCCT CAGCTCTGCC
    CAGGCCGACT TCAGCCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGTGTGGCC
    TCTAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACGTGGAG GAACCTGCAG
    AAGATCATCA AGGAGCGGGA GCTGGAGCTG CAAAAGGAGC AGCGGCGACA GGAGGAGAAT
    GACAAGCTAC GCCAGGAGTT CGCCCAGCAC GCTAACGCCT TCCACCAGTG GATACAGGAA
    ACCAGAACAT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCCGGGAC CCTGGAATCC
    CAGCTTGAGG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG
    AAGATTGAGG ACCTGGGGGC TGCCATGGAG GAGGCCCTGA TCCTGGATAA CAAGTATACA
    GAGCACAGCA CCGTGGGCCT GGCCCAACAG TGGGACCAGC TCGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACGGAGGAG
    GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC AGGCCGGCTG
    AACCACCAGG AGTTCAAGTC CTGCCTGCGC TCCCTGGGTT ATGACCTGCC CATGGTGGAG
    GAAGGGGAGC CTGACCCCGA GTTCGAGGCA ATCCTAGACA CCGTGGATCC AAACAGGGAT
    GGCCACGTCT CCCTGCAAGA GTACATGGCC TTCATGATCA GCCGTGAAAC GGAGAACGTT
    AAATCCAGTG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAGGG AAAGCCCTAC
    GTGACCAAGG AGGAGCTGTA TCAGAACCTG ACTCGAGAAC AAGCCGACTA CTGCGTCTCC
    CACATGAAGC CCTACGTGGA TGGCAAGGGC CGCGAACTCC CCACTGCCTT CGACTACGTG
    GAGTTCACCC GCTCGCTCTT TGTGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12761
    Clone ID Related Accession (Same CDS sequence) XM_005625287.3 , XM_005625287.2
    Accession Version XM_005625287.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625287.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGAGCAAA
    CACCAGAAGC ACCAGGCCTT TGAGGCGGAA CTGCATGCCA ATGCTGACCG GATCCGCGGG
    GTCATCGACA CGGGCAACTC CCTCATCGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GGCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA AAAGTCAGCC
    GAAAAGAGCC AGAAACTAAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGAATCAAG
    GACTTTGACT TCTGGCTTTC TGAGGTGGAG GCCCTTCTGG CATCTGAGGA CTACGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAG AAACATCAAC TGCTAGAGGC GGACATATCT
    GCCCACGAGG ATCGCCTGAA GGACCTGAAT AGCCAAGCAG ACAGCCTGAT GACCAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAGGATAAG CGGGACACCA TCAATGGTCG TTTCCAGAAG
    ATCAAGAACA TGGCGGCCTC CCGGCGAGCC AGGCTGAATG AATCCCATCG CCTACACCAG
    TTCTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AGAAAAAGTT GCTGGTGAGC
    TCAGAGGACT ATGGCCGAGA CCTTACAGGA GTTCAGAACC TGAGGAAGAA ACACAAGCGG
    CTGGAGGCGG AACTGGCTGC CCATGAGCCA GCGATCCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAATAC CATTGGCAAG GAGGAGATTC AGCAGCGTCT GGCCCAGTTC
    GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCGGCAGCTC GGGGTCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAT GTGGAAGAAG AAGAAGCCTG GATTAATGAG
    AAGATGACCC TGGTAGCCAG TGAGGATTAT GGAGACACGC TTGCCGCTAT CCAGGGCTTA
    CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACTGTGC ACAAGGATCG TGTGAACGAC
    GTCTGCAGTA ATGGACAAGA CCTCATCAAG AAGAACAATC ACCACGAGGA GAACATCTCT
    TCAAAGATGA AGGGCTTGAA TGGCAAAGTG TCAGACTTGG AGAAAGCTGC TGCCCAGAGG
    AAGGCCAAGC TTGAGGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC TGATGTAGTG
    GAATCCTGGA TCGGTGAGAA GGAAAACAGC CTGAAGACAG ACGATTATGG CCGAGACCTG
    TCCTCCGTGC AGACTCTCCT CACCAAACAG GAAACTTTTG ACGCTGGTCT ACAGGCCTTC
    CAGCAGGAGG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC
    ATTCAGTCAA AGGCCATTGA AGCCCGGCAC GCCTCTCTCA TGAAGAGATG GAGCCAGCTT
    CTGGCCAACT CGGCCACCCG CAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG
    GTGGAAGACC TCTTCCTGAC CTTCGCCAAA AAGGCATCGG CCTTCAACAG CTGGTTTGAA
    AACGCCGAAG AGGATCTGAC TGATCCTGTG CGTTGCAACT CCCTGGAGGA GATCAGAGCT
    CTGCGTGAGG CCCACGACGC CTTCCGCTCA TCCCTCAGCT CTGCCCAGGC CGACTTCAGC
    CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGTG TGGCCTCTAA CCCCTACACC
    TGGTTCACCA TGGAGGCCCT GGAGGAGACG TGGAGGAACC TGCAGAAGAT CATCAAGGAG
    CGGGAGCTGG AGCTGCAAAA GGAGCAGCGG CGACAGGAGG AGAATGACAA GCTACGCCAG
    GAGTTCGCCC AGCACGCTAA CGCCTTCCAC CAGTGGATAC AGGAAACCAG AACATATCTC
    CTCGATGGGT CCTGCATGGT GGAAGAATCC GGGACCCTGG AATCCCAGCT TGAGGCTACC
    AAACGCAAGC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAGGACCTG
    GGGGCTGCCA TGGAGGAGGC CCTGATCCTG GATAACAAGT ATACAGAGCA CAGCACCGTG
    GGCCTGGCCC AACAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG
    GAGCAGCAGA TCCAGGCCAG GAACACAACA GGAGTGACGG AGGAGGCCCT CAAAGAATTC
    AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCAGGCC GGCTGAACCA CCAGGAGTTC
    AAGTCCTGCC TGCGCTCCCT GGGTTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCTGAC
    CCCGAGTTCG AGGCAATCCT AGACACCGTG GATCCAAACA GGGATGGCCA CGTCTCCCTG
    CAAGAGTACA TGGCCTTCAT GATCAGCCGT GAAACGGAGA ACGTTAAATC CAGTGAGGAG
    ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAGGGAAAGC CCTACGTGAC CAAGGAGGAG
    CTGTATCAGA ACCTGACTCG AGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC
    GTGGATGGCA AGGGCCGCGA ACTCCCCACT GCCTTCGACT ACGTGGAGTT CACCCGCTCG
    CTCTTTGTGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625344.1
    CDS137..7531
    Translation

    Target ORF information:

    RefSeq Version XM_005625287.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625287.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGAGCAAA
    CACCAGAAGC ACCAGGCCTT TGAGGCGGAA CTGCATGCCA ATGCTGACCG GATCCGCGGG
    GTCATCGACA CGGGCAACTC CCTCATCGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GGCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA AAAGTCAGCC
    GAAAAGAGCC AGAAACTAAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGAATCAAG
    GACTTTGACT TCTGGCTTTC TGAGGTGGAG GCCCTTCTGG CATCTGAGGA CTACGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAG AAACATCAAC TGCTAGAGGC GGACATATCT
    GCCCACGAGG ATCGCCTGAA GGACCTGAAT AGCCAAGCAG ACAGCCTGAT GACCAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAGGATAAG CGGGACACCA TCAATGGTCG TTTCCAGAAG
    ATCAAGAACA TGGCGGCCTC CCGGCGAGCC AGGCTGAATG AATCCCATCG CCTACACCAG
    TTCTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AGAAAAAGTT GCTGGTGAGC
    TCAGAGGACT ATGGCCGAGA CCTTACAGGA GTTCAGAACC TGAGGAAGAA ACACAAGCGG
    CTGGAGGCGG AACTGGCTGC CCATGAGCCA GCGATCCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAATAC CATTGGCAAG GAGGAGATTC AGCAGCGTCT GGCCCAGTTC
    GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCGGCAGCTC GGGGTCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAT GTGGAAGAAG AAGAAGCCTG GATTAATGAG
    AAGATGACCC TGGTAGCCAG TGAGGATTAT GGAGACACGC TTGCCGCTAT CCAGGGCTTA
    CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACTGTGC ACAAGGATCG TGTGAACGAC
    GTCTGCAGTA ATGGACAAGA CCTCATCAAG AAGAACAATC ACCACGAGGA GAACATCTCT
    TCAAAGATGA AGGGCTTGAA TGGCAAAGTG TCAGACTTGG AGAAAGCTGC TGCCCAGAGG
    AAGGCCAAGC TTGAGGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC TGATGTAGTG
    GAATCCTGGA TCGGTGAGAA GGAAAACAGC CTGAAGACAG ACGATTATGG CCGAGACCTG
    TCCTCCGTGC AGACTCTCCT CACCAAACAG GAAACTTTTG ACGCTGGTCT ACAGGCCTTC
    CAGCAGGAGG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC
    ATTCAGTCAA AGGCCATTGA AGCCCGGCAC GCCTCTCTCA TGAAGAGATG GAGCCAGCTT
    CTGGCCAACT CGGCCACCCG CAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG
    GTGGAAGACC TCTTCCTGAC CTTCGCCAAA AAGGCATCGG CCTTCAACAG CTGGTTTGAA
    AACGCCGAAG AGGATCTGAC TGATCCTGTG CGTTGCAACT CCCTGGAGGA GATCAGAGCT
    CTGCGTGAGG CCCACGACGC CTTCCGCTCA TCCCTCAGCT CTGCCCAGGC CGACTTCAGC
    CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGTG TGGCCTCTAA CCCCTACACC
    TGGTTCACCA TGGAGGCCCT GGAGGAGACG TGGAGGAACC TGCAGAAGAT CATCAAGGAG
    CGGGAGCTGG AGCTGCAAAA GGAGCAGCGG CGACAGGAGG AGAATGACAA GCTACGCCAG
    GAGTTCGCCC AGCACGCTAA CGCCTTCCAC CAGTGGATAC AGGAAACCAG AACATATCTC
    CTCGATGGGT CCTGCATGGT GGAAGAATCC GGGACCCTGG AATCCCAGCT TGAGGCTACC
    AAACGCAAGC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAGGACCTG
    GGGGCTGCCA TGGAGGAGGC CCTGATCCTG GATAACAAGT ATACAGAGCA CAGCACCGTG
    GGCCTGGCCC AACAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG
    GAGCAGCAGA TCCAGGCCAG GAACACAACA GGAGTGACGG AGGAGGCCCT CAAAGAATTC
    AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCAGGCC GGCTGAACCA CCAGGAGTTC
    AAGTCCTGCC TGCGCTCCCT GGGTTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCTGAC
    CCCGAGTTCG AGGCAATCCT AGACACCGTG GATCCAAACA GGGATGGCCA CGTCTCCCTG
    CAAGAGTACA TGGCCTTCAT GATCAGCCGT GAAACGGAGA ACGTTAAATC CAGTGAGGAG
    ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAGGGAAAGC CCTACGTGAC CAAGGAGGAG
    CTGTATCAGA ACCTGACTCG AGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC
    GTGGATGGCA AGGGCCGCGA ACTCCCCACT GCCTTCGACT ACGTGGAGTT CACCCGCTCG
    CTCTTTGTGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe12761
    Clone ID Related Accession (Same CDS sequence) XM_005625287.3 , XM_005625287.2
    Accession Version XM_005625287.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726076.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGAGCAAA
    CACCAGAAGC ACCAGGCCTT TGAGGCGGAA CTGCATGCCA ATGCTGACCG GATCCGCGGG
    GTCATCGACA CGGGCAACTC CCTCATCGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GGCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA AAAGTCAGCC
    GAAAAGAGCC AGAAACTAAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGAATCAAG
    GACTTTGACT TCTGGCTTTC TGAGGTGGAG GCCCTTCTGG CATCTGAGGA CTACGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAG AAACATCAAC TGCTAGAGGC GGACATATCT
    GCCCACGAGG ATCGCCTGAA GGACCTGAAT AGCCAAGCAG ACAGCCTGAT GACCAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAGGATAAG CGGGACACCA TCAATGGTCG TTTCCAGAAG
    ATCAAGAACA TGGCGGCCTC CCGGCGAGCC AGGCTGAATG AATCCCATCG CCTACACCAG
    TTCTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AGAAAAAGTT GCTGGTGAGC
    TCAGAGGACT ATGGCCGAGA CCTTACAGGA GTTCAGAACC TGAGGAAGAA ACACAAGCGG
    CTGGAGGCGG AACTGGCTGC CCATGAGCCA GCGATCCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAATAC CATTGGCAAG GAGGAGATTC AGCAGCGTCT GGCCCAGTTC
    GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCGGCAGCTC GGGGTCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAT GTGGAAGAAG AAGAAGCCTG GATTAATGAG
    AAGATGACCC TGGTAGCCAG TGAGGATTAT GGAGACACGC TTGCCGCTAT CCAGGGCTTA
    CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACTGTGC ACAAGGATCG TGTGAACGAC
    GTCTGCAGTA ATGGACAAGA CCTCATCAAG AAGAACAATC ACCACGAGGA GAACATCTCT
    TCAAAGATGA AGGGCTTGAA TGGCAAAGTG TCAGACTTGG AGAAAGCTGC TGCCCAGAGG
    AAGGCCAAGC TTGAGGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC TGATGTAGTG
    GAATCCTGGA TCGGTGAGAA GGAAAACAGC CTGAAGACAG ACGATTATGG CCGAGACCTG
    TCCTCCGTGC AGACTCTCCT CACCAAACAG GAAACTTTTG ACGCTGGTCT ACAGGCCTTC
    CAGCAGGAGG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC
    ATTCAGTCAA AGGCCATTGA AGCCCGGCAC GCCTCTCTCA TGAAGAGATG GAGCCAGCTT
    CTGGCCAACT CGGCCACCCG CAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG
    GTGGAAGACC TCTTCCTGAC CTTCGCCAAA AAGGCATCGG CCTTCAACAG CTGGTTTGAA
    AACGCCGAAG AGGATCTGAC TGATCCTGTG CGTTGCAACT CCCTGGAGGA GATCAGAGCT
    CTGCGTGAGG CCCACGACGC CTTCCGCTCA TCCCTCAGCT CTGCCCAGGC CGACTTCAGC
    CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGTG TGGCCTCTAA CCCCTACACC
    TGGTTCACCA TGGAGGCCCT GGAGGAGACG TGGAGGAACC TGCAGAAGAT CATCAAGGAG
    CGGGAGCTGG AGCTGCAAAA GGAGCAGCGG CGACAGGAGG AGAATGACAA GCTACGCCAG
    GAGTTCGCCC AGCACGCTAA CGCCTTCCAC CAGTGGATAC AGGAAACCAG AACATATCTC
    CTCGATGGGT CCTGCATGGT GGAAGAATCC GGGACCCTGG AATCCCAGCT TGAGGCTACC
    AAACGCAAGC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAGGACCTG
    GGGGCTGCCA TGGAGGAGGC CCTGATCCTG GATAACAAGT ATACAGAGCA CAGCACCGTG
    GGCCTGGCCC AACAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG
    GAGCAGCAGA TCCAGGCCAG GAACACAACA GGAGTGACGG AGGAGGCCCT CAAAGAATTC
    AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCAGGCC GGCTGAACCA CCAGGAGTTC
    AAGTCCTGCC TGCGCTCCCT GGGTTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCTGAC
    CCCGAGTTCG AGGCAATCCT AGACACCGTG GATCCAAACA GGGATGGCCA CGTCTCCCTG
    CAAGAGTACA TGGCCTTCAT GATCAGCCGT GAAACGGAGA ACGTTAAATC CAGTGAGGAG
    ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAGGGAAAGC CCTACGTGAC CAAGGAGGAG
    CTGTATCAGA ACCTGACTCG AGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC
    GTGGATGGCA AGGGCCGCGA ACTCCCCACT GCCTTCGACT ACGTGGAGTT CACCCGCTCG
    CTCTTTGTGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625344.1
    CDS119..7513
    Translation

    Target ORF information:

    RefSeq Version XM_005625287.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625287.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CAGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTAGACAG ATACCACCGC
    TTCAAGGAGC TCTCAACCCT TAGGCGCCAG AAGCTTGAAG ATTCCTATCG ATTCCAGTTT
    TTCCAGAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAACTTCA GATCGCGTCA
    GATGAGAATT ATAAAGACCC AACCAACTTG CAGGGCAAGC TTCAGAAGCA TCAGGCCTTC
    GAAGCTGAAG TGCAGGCCAA CTCAGGAGCC ATTGTTAAAC TGGATGAAAC TGGAAACCTG
    ATGATCTCAG AAGGGCATTT TGCGTCTGAA ACCATAAGGA CCCGTTTAAT GGAGCTGCAC
    CGCCAGTGGG AGCTGCTTTT GGAGAAGATG CGAGAGAAGG GAGTCAAACT GCTGCAGGCC
    CAGAAGCTGG TGCAGTATTT GCGAGAGTGT GAGGATGTGA TGGACTGGAT CAACGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAAGACCTGG AGCATGTAGA GGTTTTACAG
    AAGAAATTTG AAGAGTTTCA AACAGATATG GCTGCCCATG AAGAAAGAGT TAATGAAGTT
    AATCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCCG AGGAGGAGAC CATCAAGACC
    AAGCAAGATG AAGTAAATGC GGCCTGGCAG CGGCTGAAGG GACTGGCCCT TCAGAGACAG
    GGGAAGCTCT TCGGAGCTGC TGAGGTTCAG CGCTTTAACA GGGATGTGGA TGAAACCATC
    AGCTGGATTA AAGAAAAGGA GCAATTAATG GCCTCTGACG ATTTTGGCCG AGACCTGGCA
    AGTGTTCAGG CTCTGCTGCG GAAGCACGAA GGCTTGGAGA GAGATCTTGC TGCTTTGGAG
    GACAAGGTCA AAGCCCTGTG TGCCGAGGCC GACCGCCTGC AGCAGTCCCA CCCTCTGAGT
    GCTGCACAGA TTCAAGTGAA GCGAGAGGAG CTGATTGCCA ACTGGGAGCA GATCCGGACT
    TTGGCAGCTG AGAGACATGC CCGCCTCAAT GACTCCTACA GGCTTCAACG CTTCCTTGCT
    GACTTCCGTG ACCTTACCAG CTGGGTAACT GAGATGAAAG CCCTTATCAA TGCAGATGAG
    CTTGCCAATG ATGTGGCCGG CGCCGAAGCC TTGCTGGACA GGCATCAAGA ACACAAGGGT
    GAGATTGATG CTCACGAGGA CAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT
    GCTGCTGGGC ATTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT GGCTGAGGAG
    AGGGCCGCCC TGCTGGAGCT GTGGGAGCTG CGCCGGCAGC AGTATGAGCA GTGCATGGAC
    CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCC
    TTCCTGCTGA ATGAGGACTT GGGAGACTCT CTGGATAGCG TGGAGGCGCT CCTCAAGAAG
    CATGAGGACT TTGAGAAATC CCTCAGCGCC CAGGAGGAAA AAATTACAGC ACTGGATGAA
    TTTGCAACCA AGCTGATTCA GAACAACCAC TACGCCATGG AAGACGTGGC TACCCGCCGA
    GATGCGCTCC TGAGCCGCCG CAACGCTCTT CATGAAAGAG CCATGTGTCG TCGCGCCCAA
    CTGGCAGACT CTTTCCACCT GCAGCAATTT TTCCGTGACT CTGATGAGCT CAAGAGCTGG
    GTCAATGAGA AGATGAAAAC CGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA
    GGAAAAGTAC AGAAGCATCA GGCTTTTGAG GCTGAGCTCT CGGCCAACCA GAGCCGCATT
    GACGCCCTGG AGAAAGCAGG GCAAAAGCTG ATTGATGTCA ACCACTATGC CAAAGATGAG
    GTAGCAGCTC GTATGAACGA GGTCATCAGT TTGTGGAAGA AATTGCTTGA AGCCACTGAA
    CTGAAGGGGA TAAAGCTTCG TGAGGCGAAC CAGCAGCAGC AGTTCAACCG CAATGTTGAG
    GATATTGAGC TATGGCTGTA TGAAGTGGAA GGTCACTTGG CTTCAGACGA TTATGGCAAA
    GACCTTACCA ATGTCCAGAA CCTCCAGAAG AAGCATGCCC TGCTAGAGGC CGATGTGGCT
    GCTCACCAGG ATCGAATCGA TGGCATCACC ATTCAGGCCC GCCAGTTCCA GGATGCTGGC
    CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAAGCCC TTGTGGCTCG CTATGAGGCT
    CTCAAAGAAC CCATGATTGC CCGAAAGCAG AAGCTGGCTG ATTCTCTACG CTTGCAGCAG
    CTCTTCCGTG ATGTTGAGGA TGAGGAGACG TGGATTAGGG AGAAAGAGCC CATCGCTGCG
    TCCACCAACA GAGGCAAAGA TTTGATTGGA GTCCAAAATC TGCTGAAGAA ACACCAAGCC
    CTACAAGCAG AAATTGCTGG GCATGAACCC CGCATCAAGG CGGTTACACA GAAGGGGAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCCGCT GAGGATGTGA AGGCTAAGCT CAGCGAACTG
    AACCAGAAGT GGGAGTCCCT CAAGTCCAAG GCCTCCCAGC GGCGGCAGGA CCTGGAAGAC
    TCCTTGCAGG CCCAGCAGTA CTTTGCTGAC GCCAACGAGG CAGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TCGTGGGCAG CACTGACTAC GGGAAGGACG AGGACTCCGC TGAGGCTCTA
    CTGAAGAAAC ATGAAGCTTT GATGTCAGAT CTCAGCGCCT ACGGCAGCAG CATCCAGGCC
    CTGCGGGACC AGGCTCAGTC CTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG
    AAGGAGCTGG TCTTGGCTCT CTACGACTAT CAGGAGAAGA GCCCTCGAGA GGTTACCATG
    AAGAAAGGGG ACATTCTCAC TTTACTCAAC AGCACCAACA AGGACTGGTG GAAGGTGGAA
    GTGAACGACC GTCAGGGTTT CGTGCCAGCC GCGTATGTGA AGAAGCTGGA CCCTGCCCAG
    TCTGCTTCCC GAGAGAACCT TCTGGAGGAG CAGGGCAGCA TTGCTGTGCG GCAGGAGCAG
    ATTGACCACC AGTATCACTC TCTGCTGGAG CTGGGCGAGA AGCGCAAGGG CATGTTGGAG
    AAGAGCTGTA AGCGGTTCAT GTTGTTCCGT GAGGCGAACG AGCTGCAGCA GTGGATCAAC
    GAGAAGGAGG CAGCTCTGAC CAGCGAGGAG GTGGGCGCTG ACCTGGAGCA GGTCGAAGTG
    CTGCAGAAGA AGTTCGATGA CTTCCAGAAG GATCTGAAGG CCAATGAGTC ACGGCTGAAA
    GACATTAACA AGGTGGCTGA AGACCTGGAG TCTGAAGGTC TGATGGCTGA GGAGGTGCAG
    GCAGTACAGC AGCAGGAGGT GTATGGCATG ATGCCCAGGG ATGACACGGA TTCCAAGACT
    GCATCCCCTT GGAAGTCTGC TCGTCTGATG GTTCATACCG TGGCCACCTT TAATTCCATC
    AAGGAGCTGA ACGAGCGTTG GCGGTCCCTG CAGCAGCTGG CCGAGGAGCG GAGCCAGCTC
    TTGGGCAGCG CCCATGAAGT ACAGAGGTTC CACAGAGATG CCGATGAAAC CAAAGAGTGG
    ATTGAGGAGA AGAATCAGGC CCTAAACACA GACAACTATG GCCACGATCT GGCCAGCGTA
    CAAGCTCTGC AGCGCAAGCA CGAGGGCTTT GAGAGGGACC TCGCGGCTCT TGGTGACAAG
    GTCAACTCCC TGGGGGAAAC AGCCGAGCGC CTGATCCAGT CCCACCCGGA GTCGGCCGAG
    GACCTGCAGG AAAAGTGCAC GGAGTTGGAC CAGGCGTGGA GCAGCCTGGG GAAGCGCGCA
    GACCAGCGCA AGGCCAAGCT GGGTGATTCC CACGACCTGC AACGCTTCCT TAGTGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TGTCCTCAGA CGAGTTAGCC
    AAGGATGTCA CCGGAGCAGA AGCTTTGCTG GAACGGCACC AGGAGCACCG GACAGAAATC
    GATGCCAGGG CTGGCACTTT CCAGGCCTTT GAGCAGTTTG GGCAGCAGCT GCTGGCTCAT
    GGGCACTACG CCAGCCCAGA GGTCAAAGAG AAACTAGATA TTCTAGATCG GGAGCGTGCG
    GGTCTGGAGA AGGCCTGGGC CCAGCGTAGG ATGATGTTGG ATCAGTGCCT TGAACTGCAG
    CTGTTTCATC GGGACTGTGA ACAAGCTGAG AACTGGATGG CCGCCCGGGA GGCCTTTCTG
    AATACTGAGG ACAAAGGAGA CTCACTGGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG
    GATTTTGACA AAGCGATCAA TGTCCAGGAG GAGAAGATTG CCGCCTTGCA GTCCTTTGCT
    GACCAACTTA TTGCTGCCGG CCACTATGCC AAGGGTGACA TCTGCAGTCG GCGCAATGAG
    GTTTTGGACA GGTGGCGACG CCTAAAAGCT CAGATGATTG AGAAGAGATC GAAGCTGGGA
    GAATCACAAA CCCTCCAGCA GTTCAGCCGG GACGTGGATG AGATCGAGGC TTGGATCAGT
    GAGAAATTAC AGACTGCGAG TGATGAGTCG TACAAAGACC CCACCAACAT CCAGAGCAAA
    CACCAGAAGC ACCAGGCCTT TGAGGCGGAA CTGCATGCCA ATGCTGACCG GATCCGCGGG
    GTCATCGACA CGGGCAACTC CCTCATCGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GGCTGGCTGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA AAAGTCAGCC
    GAAAAGAGCC AGAAACTAAA AGAAGCCAAC AAGCAGCAGA ACTTCAACAC TGGAATCAAG
    GACTTTGACT TCTGGCTTTC TGAGGTGGAG GCCCTTCTGG CATCTGAGGA CTACGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAG AAACATCAAC TGCTAGAGGC GGACATATCT
    GCCCACGAGG ATCGCCTGAA GGACCTGAAT AGCCAAGCAG ACAGCCTGAT GACCAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAGGATAAG CGGGACACCA TCAATGGTCG TTTCCAGAAG
    ATCAAGAACA TGGCGGCCTC CCGGCGAGCC AGGCTGAATG AATCCCATCG CCTACACCAG
    TTCTTCCGGG ACATGGATGA TGAGGAGTCC TGGATCAAGG AGAAAAAGTT GCTGGTGAGC
    TCAGAGGACT ATGGCCGAGA CCTTACAGGA GTTCAGAACC TGAGGAAGAA ACACAAGCGG
    CTGGAGGCGG AACTGGCTGC CCATGAGCCA GCGATCCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAATAC CATTGGCAAG GAGGAGATTC AGCAGCGTCT GGCCCAGTTC
    GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCGGCAGCTC GGGGTCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAT GTGGAAGAAG AAGAAGCCTG GATTAATGAG
    AAGATGACCC TGGTAGCCAG TGAGGATTAT GGAGACACGC TTGCCGCTAT CCAGGGCTTA
    CTGAAGAAAC ATGAAGCTTT TGAGACAGAC TTCACTGTGC ACAAGGATCG TGTGAACGAC
    GTCTGCAGTA ATGGACAAGA CCTCATCAAG AAGAACAATC ACCACGAGGA GAACATCTCT
    TCAAAGATGA AGGGCTTGAA TGGCAAAGTG TCAGACTTGG AGAAAGCTGC TGCCCAGAGG
    AAGGCCAAGC TTGAGGAGAA CTCGGCCTTC CTTCAGTTCA ACTGGAAGGC TGATGTAGTG
    GAATCCTGGA TCGGTGAGAA GGAAAACAGC CTGAAGACAG ACGATTATGG CCGAGACCTG
    TCCTCCGTGC AGACTCTCCT CACCAAACAG GAAACTTTTG ACGCTGGTCT ACAGGCCTTC
    CAGCAGGAGG GCATTGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAC
    ATTCAGTCAA AGGCCATTGA AGCCCGGCAC GCCTCTCTCA TGAAGAGATG GAGCCAGCTT
    CTGGCCAACT CGGCCACCCG CAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG
    GTGGAAGACC TCTTCCTGAC CTTCGCCAAA AAGGCATCGG CCTTCAACAG CTGGTTTGAA
    AACGCCGAAG AGGATCTGAC TGATCCTGTG CGTTGCAACT CCCTGGAGGA GATCAGAGCT
    CTGCGTGAGG CCCACGACGC CTTCCGCTCA TCCCTCAGCT CTGCCCAGGC CGACTTCAGC
    CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGTG TGGCCTCTAA CCCCTACACC
    TGGTTCACCA TGGAGGCCCT GGAGGAGACG TGGAGGAACC TGCAGAAGAT CATCAAGGAG
    CGGGAGCTGG AGCTGCAAAA GGAGCAGCGG CGACAGGAGG AGAATGACAA GCTACGCCAG
    GAGTTCGCCC AGCACGCTAA CGCCTTCCAC CAGTGGATAC AGGAAACCAG AACATATCTC
    CTCGATGGGT CCTGCATGGT GGAAGAATCC GGGACCCTGG AATCCCAGCT TGAGGCTACC
    AAACGCAAGC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAGGACCTG
    GGGGCTGCCA TGGAGGAGGC CCTGATCCTG GATAACAAGT ATACAGAGCA CAGCACCGTG
    GGCCTGGCCC AACAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG
    GAGCAGCAGA TCCAGGCCAG GAACACAACA GGAGTGACGG AGGAGGCCCT CAAAGAATTC
    AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCAGGCC GGCTGAACCA CCAGGAGTTC
    AAGTCCTGCC TGCGCTCCCT GGGTTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCTGAC
    CCCGAGTTCG AGGCAATCCT AGACACCGTG GATCCAAACA GGGATGGCCA CGTCTCCCTG
    CAAGAGTACA TGGCCTTCAT GATCAGCCGT GAAACGGAGA ACGTTAAATC CAGTGAGGAG
    ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAGGGAAAGC CCTACGTGAC CAAGGAGGAG
    CTGTATCAGA ACCTGACTCG AGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC
    GTGGATGGCA AGGGCCGCGA ACTCCCCACT GCCTTCGACT ACGTGGAGTT CACCCGCTCG
    CTCTTTGTGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.