EFCAB8 gene cDNA ORF clone, Canis lupus familiaris(Dog)

mRNA	Protein	Name
XM_022408909.1	XP_022264617.1	EF-hand calcium-binding domain-containing protein 8 isoform X10
XM_022408911.1	XP_022264619.1	EF-hand calcium-binding domain-containing protein 8 isoform X11
XM_014107219.1	XP_013962694.1	EF-hand calcium-binding domain-containing protein 8 isoform X1
XM_014107220.1	XP_013962695.1	EF-hand calcium-binding domain-containing protein 8 isoform X2
XM_014107225.1	XP_013962700.1	EF-hand calcium-binding domain-containing protein 8 isoform X6
XM_014107227.1	XP_013962702.1	EF-hand calcium-binding domain-containing protein 8 isoform X8
XM_014107220.2	XP_013962695.1	EF-hand calcium-binding domain-containing protein 8 isoform X2
XM_014107226.2	XP_013962701.1	EF-hand calcium-binding domain-containing protein 8 isoform X7
XM_014107227.2	XP_013962702.1	EF-hand calcium-binding domain-containing protein 8 isoform X9
XM_014107228.1	XP_013962703.1	EF-hand calcium-binding domain-containing protein 8 isoform X9
XM_014107222.2	XP_013962697.1	EF-hand calcium-binding domain-containing protein 8 isoform X4
XM_022408907.1	XP_022264615.1	EF-hand calcium-binding domain-containing protein 8 isoform X5
XM_014107222.1	XP_013962697.1	EF-hand calcium-binding domain-containing protein 8 isoform X4
XM_014107221.2	XP_013962696.1	EF-hand calcium-binding domain-containing protein 8 isoform X3
XM_022408910.1	XP_022264618.1	EF-hand calcium-binding domain-containing protein 8 isoform X11
XM_014107221.1	XP_013962696.1	EF-hand calcium-binding domain-containing protein 8 isoform X3
XM_014107226.1	XP_013962701.1	EF-hand calcium-binding domain-containing protein 8 isoform X7
XM_014107219.2	XP_013962694.1	EF-hand calcium-binding domain-containing protein 8 isoform X1
XM_014107224.2	XP_013962699.1	EF-hand calcium-binding domain-containing protein 8 isoform X6
XM_022408908.1	XP_022264616.1	EF-hand calcium-binding domain-containing protein 8 isoform X8
XM_014107224.1	XP_013962699.1	EF-hand calcium-binding domain-containing protein 8 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe296636	XM_022408907.1 Latest version!	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe296637	XM_022408908.1 Latest version!	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe296638	XM_022408909.1 Latest version!	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe296639	XM_022408910.1 Latest version!	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe296639	XM_022408911.1 Latest version!	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe42868	XM_014107219.2 Latest version!	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42869	XM_014107220.2 Latest version!	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42870	XM_014107221.2 Latest version!	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42871	XM_014107222.2 Latest version!	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42872	XM_014107224.2 Latest version!	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42873	XM_014107225.1 Latest version!	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42874	XM_014107226.2 Latest version!	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42875	XM_014107227.2 Latest version!	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42876	XM_014107228.1 Latest version!	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OCe42869	XM_014107220.1	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42875	XM_014107227.1	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42871	XM_014107222.1	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42870	XM_014107221.1	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42874	XM_014107226.1	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42868	XM_014107219.1	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe42872	XM_014107224.1	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

EFCAB8 ( XM_022408907.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022264615.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe296636
Clone ID Related Accession (Same CDS sequence)	XM_022408907.1
Accession Version	XM_022408907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTCG AAAACCAATA TATTTCTGTC AAAAATAGCC AGCAAGGTTA CTTGGGAAGA 
ACAACTTGGG TCAGGGCATC CACCCCAGGT GGATTCCAGA AGAATGAGGA GGTTCTTAGC 
TCCCCAGCAT CAACTGTCAC TCTTAGCCAT CATGAGTCTC AACTGTTTAC CCAGCTACAC 
ATGGTCAAGA TGGAGAAAAT GTTTGAGGAT GATGTTGACT CGACTGGAGC CCTGGACATG 
AAGACTTTCA TTAAGGCCAT AAAGAAGATT CTGAGCAATG CGTCAGATGA GATGCTAGAG 
GCGCTGTTTT TGAAGGTGGA CACAGACTGT AATGGCTCCA TTACCTGGCA GGAGTACGTG 
GATTACGTGA TGCGGGAGTT ACAGAGAAGG GAGAGGATGA GAACAAGCCA GTACCGCCTG 
CACTTCCACC TTCCCATGAG GATCATTCCC CTGAACCATG GATGCGAGAT TGTGAAGGTG 
CAGTTCTTAA CTCAGCGGTT CAAGAAGATG GGGCACCTTC TGACTGTCAC CAAGGACGGG 
ATCTTGCAGA TCTGGTCTGA ATCCTTCTCG CTGCTTAACT CCTTTAGGCT TAACCAGATC 
CATCAGCCCT ATAACCAGCA GATGTGGGTC ACTGATATGG TGTGTCTCCA TAACATGAAC 
CTGATTGCGA TTGCATCAAC TGACCAGAAA ATAGAGTTCT TTGATATTAC CAACCATAAT 
TGTGCCCGGG CCTTCACCTT CATTGATCTG GACAGCTGTG TCCTGACCAT GGACTATTGG 
TCCGACTATC ACAGAGGTGT ATTCTGCTAC GGGGACACCA AAGGCAATGT CATTGTCTTC 
ACCTCTGACA ATGTGACCCA CGGGCTGTTC AACCCTCGAA TCCTTCCCAG GACCTCCAAA 
TGGGATCACT GGACCAAAGT TTCTACACGG AAGCTTCTAA ATGAGAAGTC TCCTTTGTAC 
AGGAGTTATT GGCTAAAGGC TCTCCATCCC AACTGGTGCC AACAGGTCAA GTTCATTCCT 
CAGCTGAACA CAGTGGCCTC CTGTTCGGCC ATTGAGAAGT CCTCTCTGGT GCTAATAGCA 
TTGCCATCTA AAGCCCCAGA GAGCCCCAAG TTTTCAGTGC TGAACTTAAG AAAAGGGATT 
CTTTGCTTCG ATTACTGCCC AGACAAGAAC TTAGTGGTGA CTGGTGGCTA TGACCCCCTC 
ATCCGCCTGT GGAACCCCTT CTTCTCAAAA AAGCCTGTGT GGATGATGAA GGGGCATCAG 
ACTTCCGTGA CGCACATCCT CGTGAACAGC AAGAACAGCA GCATCCTCCT CAGCATCTCC 
AAGGACAAGA ATATCCGTGT GTGGGACATG CAGGACTACA TATGCCTCCA GTCCTTTTCC 
GGGAAGCTTT TTGCCCTGGG AAACTGCCCC ATCACCAGTG TCTACTTCCA CAAGAATGAA 
GACAGTCTCA TCTGTAGCAC CTTTTCAATT GGGATCCTAA AAGGGTACTT GGAGACTGAG 
GGGCCTGGGA AACCAAGGGA GAGGACCACA ACTTATAGCA CACCCCTGTG CGCTGTTCTC 
TACAGCAAGA TTTTTAAGCA GGTGGTAAGC GGCTGCCTGA GCAGCTTGGT GAGTGTGTGG 
GAGATCAAGA CAGGCAAGAG GATGATGGAG TTCTCTGTGA CTGGCGACCA GCAAGTGGAG 
CTGACCACCA TGTGCCTGGA TGAATCCGAG CGGTGCCTGC TCACGGGTTT GCGCGACGGC 
ACCATAAAGA TGTGGAACTA CACTGTGGGT GAATGCCTGC TGACCTTCCC CAACCCGGAC 
CAGCTGGAGA TTAGCGGGAT TGTCCATATG AACAAAGTGT TCTACGTGAC TGGATGGAGT 
AAGAGAATCA CCTGTTTCAT GCTCCATAAG ACCAAGCCAG TCCTCCTGTG CTACCAATGG 
CAGAGCTTCC ACAAGGAAGA TGTTCTGAGC ATGGCCAAGT ACCAAAACCA GTTCCTTGGG 
ACCTCTTCCT ACAATGGAGA TATTCTCTTC TGGAACATCA ACCTGTTCAA GCCCATCCTC 
AATTTCAATG CTTCTAAGAG CCCCTGGCCT TTGCTGCCCA AGAAGATCCA AGAAGTGGAC 
AACTCCCTGG CTGAGAGCCT CCAGTCCAGC AAGCTTTGTG TGAAACATAA GGAGTGGGCG 
TACAAACCAT CAACGCAGCC CCTGGGCCCC AGAGCCAGGG CTGTAGCCAA TGCCAACCTG 
CAACGGAACC TGATGTCAGC TCCCCCAGTG ATGAAGCGCC CCAGAGACAA GGAGCCAGAA 
AAGCCCATGC CCCCGCAGGT GGGAGCGGAG GTGAGGCAGA AACATGTCAG TTCACAAAGC 
CCCAAGCTGT TAAGAATGCC CCATGGCAAA CAGTCCATCA TCAGAGACGC TCAAAGGAAA 
AGAGGGGAAT TCCAGAAGAA GATACTACTG CAATCCAACA CATCGGTGGA GAAGATAATC 
TTCCTGCAGA CCAGGCCTCG CCTGCCACAC ATAGCTGCCC TGCTGAGCAG CTGCATCGAT 
GGCTACATCT ATGCCTGGTC CATCCACGGG AGCGGAGGCC TGTTGGGGAA GTTCCCTGTG 
GACTTCAAAA ACCAGGGGGA TGTGGGTGTG GGTGCCATGG CCACGGATGA GAAAGACTTG 
ATCCTTGTCA CAGGAGATTG TAAAGGGCAC ATCAAGATTT GGGACATCGA GGATTATTGC 
ATGTTCACCG ACAAGACACC ACTTCAACCC AGAGGGACAA ACAAGTTCCA ATTCTTAATT 
CCTGAGCAGG TCCAGATCAA CCTGCCACAC TACATCCCCT TGAAGGAGAA GGAGGTCATG 
GCTGGCCAGA CCATTTCCCT GGTTCCCCCC AAGCTTCTGA ATGCCTGGAA GGGCCATTTG 
GATAGTGTGG CAGACATCCT GTATGTGGAT AGCTTCCAGC TGATTATCAG TGCTGGCCAC 
GATTGTGACG TCAAGGCTTG GAAACTCTCC GGTGACACCA TTGGGACGTT TGGCGTGAGT 
GTTTGGAAAG GGTTGCAGGA TATTCAGACG GTTGGTGATC TTGCACTAAG AAAAAGCTCA 
GAGGAGGAGG CTGACTCTAT AGATGCTGCC CAGAAGGCCT TCCGTCCAGA GCTGCAGGAG 
GAGCGGGCTC TTGCCGAGGC CCTGGTGTAC CAGCGGCGGG AGCAGGTGGC ACTCATGGCT 
CTCCTGAATG GGAAGGCAGA CGCGGAGGCT GAGGCTTGGG GTAAGCTGCA GAGGATAACC 
CAGATGTCCC CATGGGCTGG AGAGCACTAC TCAGAAGACG TTGAGCACAG CTGGCTCGAG 
TGGGAGTCCA AGGGCAAGCA GGTGAGCAAA ATCGTGGGAG GAGCATACAA GGCCAAGGAC 
CGGTCTCGGA GTCCCGGAGT CCTGTCCACT AATGTGCAGT ATGGCTGGAT GAAGCACCAG 
ATTTCACCTC AGATCTACCA AAGCCTGCAC TTCAACCAGC TGTCACCCAC CCACACACCT 
GACTTCCTGG TGCACAATAT CCTAGGGCAT CACGGCCAGC ACATCTACCT GGTGACCCAC 
AATAGCCAAA AGGACCTGGA TCCCGAAGAG ACCAACTCGT CTACTGCCTC TGTCTCCCCC 
AGCGACACCC TCACCCCGAA AGCCTCAGGC TCCGGGTTCC TGGAGTCCGC CTTGTCCACC 
TCCCCGTACC TCCAGCCCTC CCTCTCCCTC CTGTCCCAGC CATCCAGGTA TGTCACGCAG 
AGGTCGGCGC CCCCCATGCC TGTAGCCTCA TCTATCAAAC TGCTCTCAAG ACCTCTGAAG 
GCCGCCCTTT CATCCTCCAT GAAGAAGAGC CACGGCGTCA GGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264615.1
CDS	1154..4981
Translation	MEVENQYISVKNSQQGYLGRTTWVRASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGALDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_022408907.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408907.1

ORF Insert Sequence:

ATGGAAGTCG AAAACCAATA TATTTCTGTC AAAAATAGCC AGCAAGGTTA CTTGGGAAGA 
ACAACTTGGG TCAGGGCATC CACCCCAGGT GGATTCCAGA AGAATGAGGA GGTTCTTAGC 
TCCCCAGCAT CAACTGTCAC TCTTAGCCAT CATGAGTCTC AACTGTTTAC CCAGCTACAC 
ATGGTCAAGA TGGAGAAAAT GTTTGAGGAT GATGTTGACT CGACTGGAGC CCTGGACATG 
AAGACTTTCA TTAAGGCCAT AAAGAAGATT CTGAGCAATG CGTCAGATGA GATGCTAGAG 
GCGCTGTTTT TGAAGGTGGA CACAGACTGT AATGGCTCCA TTACCTGGCA GGAGTACGTG 
GATTACGTGA TGCGGGAGTT ACAGAGAAGG GAGAGGATGA GAACAAGCCA GTACCGCCTG 
CACTTCCACC TTCCCATGAG GATCATTCCC CTGAACCATG GATGCGAGAT TGTGAAGGTG 
CAGTTCTTAA CTCAGCGGTT CAAGAAGATG GGGCACCTTC TGACTGTCAC CAAGGACGGG 
ATCTTGCAGA TCTGGTCTGA ATCCTTCTCG CTGCTTAACT CCTTTAGGCT TAACCAGATC 
CATCAGCCCT ATAACCAGCA GATGTGGGTC ACTGATATGG TGTGTCTCCA TAACATGAAC 
CTGATTGCGA TTGCATCAAC TGACCAGAAA ATAGAGTTCT TTGATATTAC CAACCATAAT 
TGTGCCCGGG CCTTCACCTT CATTGATCTG GACAGCTGTG TCCTGACCAT GGACTATTGG 
TCCGACTATC ACAGAGGTGT ATTCTGCTAC GGGGACACCA AAGGCAATGT CATTGTCTTC 
ACCTCTGACA ATGTGACCCA CGGGCTGTTC AACCCTCGAA TCCTTCCCAG GACCTCCAAA 
TGGGATCACT GGACCAAAGT TTCTACACGG AAGCTTCTAA ATGAGAAGTC TCCTTTGTAC 
AGGAGTTATT GGCTAAAGGC TCTCCATCCC AACTGGTGCC AACAGGTCAA GTTCATTCCT 
CAGCTGAACA CAGTGGCCTC CTGTTCGGCC ATTGAGAAGT CCTCTCTGGT GCTAATAGCA 
TTGCCATCTA AAGCCCCAGA GAGCCCCAAG TTTTCAGTGC TGAACTTAAG AAAAGGGATT 
CTTTGCTTCG ATTACTGCCC AGACAAGAAC TTAGTGGTGA CTGGTGGCTA TGACCCCCTC 
ATCCGCCTGT GGAACCCCTT CTTCTCAAAA AAGCCTGTGT GGATGATGAA GGGGCATCAG 
ACTTCCGTGA CGCACATCCT CGTGAACAGC AAGAACAGCA GCATCCTCCT CAGCATCTCC 
AAGGACAAGA ATATCCGTGT GTGGGACATG CAGGACTACA TATGCCTCCA GTCCTTTTCC 
GGGAAGCTTT TTGCCCTGGG AAACTGCCCC ATCACCAGTG TCTACTTCCA CAAGAATGAA 
GACAGTCTCA TCTGTAGCAC CTTTTCAATT GGGATCCTAA AAGGGTACTT GGAGACTGAG 
GGGCCTGGGA AACCAAGGGA GAGGACCACA ACTTATAGCA CACCCCTGTG CGCTGTTCTC 
TACAGCAAGA TTTTTAAGCA GGTGGTAAGC GGCTGCCTGA GCAGCTTGGT GAGTGTGTGG 
GAGATCAAGA CAGGCAAGAG GATGATGGAG TTCTCTGTGA CTGGCGACCA GCAAGTGGAG 
CTGACCACCA TGTGCCTGGA TGAATCCGAG CGGTGCCTGC TCACGGGTTT GCGCGACGGC 
ACCATAAAGA TGTGGAACTA CACTGTGGGT GAATGCCTGC TGACCTTCCC CAACCCGGAC 
CAGCTGGAGA TTAGCGGGAT TGTCCATATG AACAAAGTGT TCTACGTGAC TGGATGGAGT 
AAGAGAATCA CCTGTTTCAT GCTCCATAAG ACCAAGCCAG TCCTCCTGTG CTACCAATGG 
CAGAGCTTCC ACAAGGAAGA TGTTCTGAGC ATGGCCAAGT ACCAAAACCA GTTCCTTGGG 
ACCTCTTCCT ACAATGGAGA TATTCTCTTC TGGAACATCA ACCTGTTCAA GCCCATCCTC 
AATTTCAATG CTTCTAAGAG CCCCTGGCCT TTGCTGCCCA AGAAGATCCA AGAAGTGGAC 
AACTCCCTGG CTGAGAGCCT CCAGTCCAGC AAGCTTTGTG TGAAACATAA GGAGTGGGCG 
TACAAACCAT CAACGCAGCC CCTGGGCCCC AGAGCCAGGG CTGTAGCCAA TGCCAACCTG 
CAACGGAACC TGATGTCAGC TCCCCCAGTG ATGAAGCGCC CCAGAGACAA GGAGCCAGAA 
AAGCCCATGC CCCCGCAGGT GGGAGCGGAG GTGAGGCAGA AACATGTCAG TTCACAAAGC 
CCCAAGCTGT TAAGAATGCC CCATGGCAAA CAGTCCATCA TCAGAGACGC TCAAAGGAAA 
AGAGGGGAAT TCCAGAAGAA GATACTACTG CAATCCAACA CATCGGTGGA GAAGATAATC 
TTCCTGCAGA CCAGGCCTCG CCTGCCACAC ATAGCTGCCC TGCTGAGCAG CTGCATCGAT 
GGCTACATCT ATGCCTGGTC CATCCACGGG AGCGGAGGCC TGTTGGGGAA GTTCCCTGTG 
GACTTCAAAA ACCAGGGGGA TGTGGGTGTG GGTGCCATGG CCACGGATGA GAAAGACTTG 
ATCCTTGTCA CAGGAGATTG TAAAGGGCAC ATCAAGATTT GGGACATCGA GGATTATTGC 
ATGTTCACCG ACAAGACACC ACTTCAACCC AGAGGGACAA ACAAGTTCCA ATTCTTAATT 
CCTGAGCAGG TCCAGATCAA CCTGCCACAC TACATCCCCT TGAAGGAGAA GGAGGTCATG 
GCTGGCCAGA CCATTTCCCT GGTTCCCCCC AAGCTTCTGA ATGCCTGGAA GGGCCATTTG 
GATAGTGTGG CAGACATCCT GTATGTGGAT AGCTTCCAGC TGATTATCAG TGCTGGCCAC 
GATTGTGACG TCAAGGCTTG GAAACTCTCC GGTGACACCA TTGGGACGTT TGGCGTGAGT 
GTTTGGAAAG GGTTGCAGGA TATTCAGACG GTTGGTGATC TTGCACTAAG AAAAAGCTCA 
GAGGAGGAGG CTGACTCTAT AGATGCTGCC CAGAAGGCCT TCCGTCCAGA GCTGCAGGAG 
GAGCGGGCTC TTGCCGAGGC CCTGGTGTAC CAGCGGCGGG AGCAGGTGGC ACTCATGGCT 
CTCCTGAATG GGAAGGCAGA CGCGGAGGCT GAGGCTTGGG GTAAGCTGCA GAGGATAACC 
CAGATGTCCC CATGGGCTGG AGAGCACTAC TCAGAAGACG TTGAGCACAG CTGGCTCGAG 
TGGGAGTCCA AGGGCAAGCA GGTGAGCAAA ATCGTGGGAG GAGCATACAA GGCCAAGGAC 
CGGTCTCGGA GTCCCGGAGT CCTGTCCACT AATGTGCAGT ATGGCTGGAT GAAGCACCAG 
ATTTCACCTC AGATCTACCA AAGCCTGCAC TTCAACCAGC TGTCACCCAC CCACACACCT 
GACTTCCTGG TGCACAATAT CCTAGGGCAT CACGGCCAGC ACATCTACCT GGTGACCCAC 
AATAGCCAAA AGGACCTGGA TCCCGAAGAG ACCAACTCGT CTACTGCCTC TGTCTCCCCC 
AGCGACACCC TCACCCCGAA AGCCTCAGGC TCCGGGTTCC TGGAGTCCGC CTTGTCCACC 
TCCCCGTACC TCCAGCCCTC CCTCTCCCTC CTGTCCCAGC CATCCAGGTA TGTCACGCAG 
AGGTCGGCGC CCCCCATGCC TGTAGCCTCA TCTATCAAAC TGCTCTCAAG ACCTCTGAAG 
GCCGCCCTTT CATCCTCCAT GAAGAAGAGC CACGGCGTCA GGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_022408908.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022264616.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe296637
Clone ID Related Accession (Same CDS sequence)	XM_022408908.1
Accession Version	XM_022408908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTT TCATTAAGGC CATAAAGAAG ATTCTGAGCA ATGCGTCAGA TGAGATGCTA 
GAGGCGCTGT TTTTGAAGGT GGACACAGAC TGTAATGGCT CCATTACCTG GCAGGAGTAC 
GTGGATTACG TGATGCGGGA GTTACAGAGA AGGGAGAGGA TGAGAACAAG CCAGTACCGC 
CTGCACTTCC ACCTTCCCAT GAGGATCATT CCCCTGAACC ATGGATGCGA GATTGTGAAG 
GTGCAGTTCT TAACTCAGCG GTTCAAGAAG ATGGGGCACC TTCTGACTGT CACCAAGGAC 
GGGATCTTGC AGATCTGGTC TGAATCCTTC TCGCTGCTTA ACTCCTTTAG GCTTAACCAG 
ATCCATCAGC CCTATAACCA GCAGATGTGG GTCACTGATA TGGTGTGTCT CCATAACATG 
AACCTGATTG CGATTGCATC AACTGACCAG AAAATAGAGT TCTTTGATAT TACCAACCAT 
AATTGTGCCC GGGCCTTCAC CTTCATTGAT CTGGACAGCT GTGTCCTGAC CATGGACTAT 
TGGTCCGACT ATCACAGAGG TGTATTCTGC TACGGGGACA CCAAAGGCAA TGTCATTGTC 
TTCACCTCTG ACAATGTGAC CCACGGGCTG TTCAACCCTC GAATCCTTCC CAGGACCTCC 
AAATGGGATC ACTGGACCAA AGTTTCTACA CGGAAGCTTC TAAATGAGAA GTCTCCTTTG 
TACAGGAGTT ATTGGCTAAA GGCTCTCCAT CCCAACTGGT GCCAACAGGT CAAGTTCATT 
CCTCAGCTGA ACACAGTGGC CTCCTGTTCG GCCATTGAGA AGTCCTCTCT GGTGCTAATA 
GCATTGCCAT CTAAAGCCCC AGAGAGCCCC AAGTTTTCAG TGCTGAACTT AAGAAAAGGG 
ATTCTTTGCT TCGATTACTG CCCAGACAAG AACTTAGTGG TGACTGGTGG CTATGACCCC 
CTCATCCGCC TGTGGAACCC CTTCTTCTCA AAAAAGCCTG TGTGGATGAT GAAGGGGCAT 
CAGACTTCCG TGACGCACAT CCTCGTGAAC AGCAAGAACA GCAGCATCCT CCTCAGCATC 
TCCAAGGACA AGAATATCCG TGTGTGGGAC ATGCAGGACT ACATATGCCT CCAGTCCTTT 
TCCGGGAAGC TTTTTGCCCT GGGAAACTGC CCCATCACCA GTGTCTACTT CCACAAGAAT 
GAAGACAGTC TCATCTGTAG CACCTTTTCA ATTGGGATCC TAAAAGGGTA CTTGGAGACT 
GAGGGGCCTG GGAAACCAAG GGAGAGGACC ACAACTTATA GCACACCCCT GTGCGCTGTT 
CTCTACAGCA AGATTTTTAA GCAGGTGGTA AGCGGCTGCC TGAGCAGCTT GGTGAGTGTG 
TGGGAGATCA AGACAGGCAA GAGGATGATG GAGTTCTCTG TGACTGGCGA CCAGCAAGTG 
GAGCTGACCA CCATGTGCCT GGATGAATCC GAGCGGTGCC TGCTCACGGG TTTGCGCGAC 
GGCACCATAA AGATGTGGAA CTACACTGTG GGTGAATGCC TGCTGACCTT CCCCAACCCG 
GACCAGCTGG AGATTAGCGG GATTGTCCAT ATGAACAAAG TGTTCTACGT GACTGGATGG 
AGTAAGAGAA TCACCTGTTT CATGCTCCAT AAGACCAAGC CAGTCCTCCT GTGCTACCAA 
TGGCAGAGCT TCCACAAGGA AGATGTTCTG AGCATGGCCA AGTACCAAAA CCAGTTCCTT 
GGGACCTCTT CCTACAATGG AGATATTCTC TTCTGGAACA TCAACCTGTT CAAGCCCATC 
CTCAATTTCA ATGCTTCTAA GAGCCCCTGG CCTTTGCTGC CCAAGAAGAT CCAAGAAGTG 
GACAACTCCC TGGCTGAGAG CCTCCAGTCC AGCAAGCTTT GTGTGAAACA TAAGGAGTGG 
GCGTACAAAC CATCAACGCA GCCCCTGGGC CCCAGAGCCA GGGCTGTAGC CAATGCCAAC 
CTGCAACGGA ACCTGATGTC AGCTCCCCCA GTGATGAAGC GCCCCAGAGA CAAGGAGCCA 
GAAAAGCCCA TGCCCCCGCA GGTGGGAGCG GAGGTGAGGC AGAAACATGT CAGTTCACAA 
AGCCCCAAGC TGTTAAGAAT GCCCCATGGC AAACAGTCCA TCATCAGAGA CGCTCAAAGG 
AAAAGAGGGG AATTCCAGAA GAAGATACTA CTGCAATCCA ACACATCGGT GGAGAAGATA 
ATCTTCCTGC AGACCAGGCC TCGCCTGCCA CACATAGCTG CCCTGCTGAG CAGCTGCATC 
GATGGCTACA TCTATGCCTG GTCCATCCAC GGGAGCGGAG GCCTGTTGGG GAAGTTCCCT 
GTGGACTTCA AAAACCAGGG GGATGTGGGT GTGGGTGCCA TGGCCACGGA TGAGAAAGAC 
TTGATCCTTG TCACAGGAGA TTGTAAAGGG CACATCAAGA TTTGGGACAT CGAGGATTAT 
TGCATGTTCA CCGACAAGAC ACCACTTCAA CCCAGAGGGA CAAACAAGTT CCAATTCTTA 
ATTCCTGAGC AGGTCCAGAT CAACCTGCCA CACTACATCC CCTTGAAGGA GAAGGAGGTC 
ATGGCTGGCC AGACCATTTC CCTGGTTCCC CCCAAGCTTC TGAATGCCTG GAAGGGCCAT 
TTGGATAGTG TGGCAGACAT CCTGTATGTG GATAGCTTCC AGCTGATTAT CAGTGCTGGC 
CACGATTGTG ACGTCAAGGC TTGGAAACTC TCCGGTGACA CCATTGGGAC GTTTGGCGTG 
AGTGTTTGGA AAGGGTTGCA GGATATTCAG ACGGTTGGTG ATCTTGCACT AAGAAAAAGC 
TCAGAGGAGG AGGCTGACTC TATAGATGCT GCCCAGAAGG CCTTCCGTCC AGAGCTGCAG 
GAGGAGCGGG CTCTTGCCGA GGCCCTGGTG TACCAGCGGC GGGAGCAGGT GGCACTCATG 
GCTCTCCTGA ATGGGAAGGC AGACGCGGAG GCTGAGGCTT GGGGTAAGCT GCAGAGGATA 
ACCCAGATGT CCCCATGGGC TGGAGAGCAC TACTCAGAAG ACGTTGAGCA CAGCTGGCTC 
GAGTGGGAGT CCAAGGGCAA GCAGGTGAGC AAAATCGTGG GAGGAGCATA CAAGGCCAAG 
GACCGGTCTC GGAGTCCCGG AGTCCTGTCC ACTAATGTGC AGTATGGCTG GATGAAGCAC 
CAGATTTCAC CTCAGATCTA CCAAAGCCTG CACTTCAACC AGCTGTCACC CACCCACACA 
CCTGACTTCC TGGTGCACAA TATCCTAGGG CATCACGGCC AGCACATCTA CCTGGTGACC 
CACAATAGCC AAAAGGACCT GGATCCCGAA GAGACCAACT CGTCTACTGC CTCTGTCTCC 
CCCAGCGACA CCCTCACCCC GAAAGCCTCA GGCTCCGGGT TCCTGGAGTC CGCCTTGTCC 
ACCTCCCCGT ACCTCCAGCC CTCCCTCTCC CTCCTGTCCC AGCCATCCAG GTATGTCACG 
CAGAGGTCGG CGCCCCCCAT GCCTGTAGCC TCATCTATCA AACTGCTCTC AAGACCTCTG 
AAGGCCGCCC TTTCATCCTC CATGAAGAAG AGCCACGGCG TCAGGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264616.1
CDS	479..4069
Translation	MKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_022408908.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408908.1

ORF Insert Sequence:

ATGAAGACTT TCATTAAGGC CATAAAGAAG ATTCTGAGCA ATGCGTCAGA TGAGATGCTA 
GAGGCGCTGT TTTTGAAGGT GGACACAGAC TGTAATGGCT CCATTACCTG GCAGGAGTAC 
GTGGATTACG TGATGCGGGA GTTACAGAGA AGGGAGAGGA TGAGAACAAG CCAGTACCGC 
CTGCACTTCC ACCTTCCCAT GAGGATCATT CCCCTGAACC ATGGATGCGA GATTGTGAAG 
GTGCAGTTCT TAACTCAGCG GTTCAAGAAG ATGGGGCACC TTCTGACTGT CACCAAGGAC 
GGGATCTTGC AGATCTGGTC TGAATCCTTC TCGCTGCTTA ACTCCTTTAG GCTTAACCAG 
ATCCATCAGC CCTATAACCA GCAGATGTGG GTCACTGATA TGGTGTGTCT CCATAACATG 
AACCTGATTG CGATTGCATC AACTGACCAG AAAATAGAGT TCTTTGATAT TACCAACCAT 
AATTGTGCCC GGGCCTTCAC CTTCATTGAT CTGGACAGCT GTGTCCTGAC CATGGACTAT 
TGGTCCGACT ATCACAGAGG TGTATTCTGC TACGGGGACA CCAAAGGCAA TGTCATTGTC 
TTCACCTCTG ACAATGTGAC CCACGGGCTG TTCAACCCTC GAATCCTTCC CAGGACCTCC 
AAATGGGATC ACTGGACCAA AGTTTCTACA CGGAAGCTTC TAAATGAGAA GTCTCCTTTG 
TACAGGAGTT ATTGGCTAAA GGCTCTCCAT CCCAACTGGT GCCAACAGGT CAAGTTCATT 
CCTCAGCTGA ACACAGTGGC CTCCTGTTCG GCCATTGAGA AGTCCTCTCT GGTGCTAATA 
GCATTGCCAT CTAAAGCCCC AGAGAGCCCC AAGTTTTCAG TGCTGAACTT AAGAAAAGGG 
ATTCTTTGCT TCGATTACTG CCCAGACAAG AACTTAGTGG TGACTGGTGG CTATGACCCC 
CTCATCCGCC TGTGGAACCC CTTCTTCTCA AAAAAGCCTG TGTGGATGAT GAAGGGGCAT 
CAGACTTCCG TGACGCACAT CCTCGTGAAC AGCAAGAACA GCAGCATCCT CCTCAGCATC 
TCCAAGGACA AGAATATCCG TGTGTGGGAC ATGCAGGACT ACATATGCCT CCAGTCCTTT 
TCCGGGAAGC TTTTTGCCCT GGGAAACTGC CCCATCACCA GTGTCTACTT CCACAAGAAT 
GAAGACAGTC TCATCTGTAG CACCTTTTCA ATTGGGATCC TAAAAGGGTA CTTGGAGACT 
GAGGGGCCTG GGAAACCAAG GGAGAGGACC ACAACTTATA GCACACCCCT GTGCGCTGTT 
CTCTACAGCA AGATTTTTAA GCAGGTGGTA AGCGGCTGCC TGAGCAGCTT GGTGAGTGTG 
TGGGAGATCA AGACAGGCAA GAGGATGATG GAGTTCTCTG TGACTGGCGA CCAGCAAGTG 
GAGCTGACCA CCATGTGCCT GGATGAATCC GAGCGGTGCC TGCTCACGGG TTTGCGCGAC 
GGCACCATAA AGATGTGGAA CTACACTGTG GGTGAATGCC TGCTGACCTT CCCCAACCCG 
GACCAGCTGG AGATTAGCGG GATTGTCCAT ATGAACAAAG TGTTCTACGT GACTGGATGG 
AGTAAGAGAA TCACCTGTTT CATGCTCCAT AAGACCAAGC CAGTCCTCCT GTGCTACCAA 
TGGCAGAGCT TCCACAAGGA AGATGTTCTG AGCATGGCCA AGTACCAAAA CCAGTTCCTT 
GGGACCTCTT CCTACAATGG AGATATTCTC TTCTGGAACA TCAACCTGTT CAAGCCCATC 
CTCAATTTCA ATGCTTCTAA GAGCCCCTGG CCTTTGCTGC CCAAGAAGAT CCAAGAAGTG 
GACAACTCCC TGGCTGAGAG CCTCCAGTCC AGCAAGCTTT GTGTGAAACA TAAGGAGTGG 
GCGTACAAAC CATCAACGCA GCCCCTGGGC CCCAGAGCCA GGGCTGTAGC CAATGCCAAC 
CTGCAACGGA ACCTGATGTC AGCTCCCCCA GTGATGAAGC GCCCCAGAGA CAAGGAGCCA 
GAAAAGCCCA TGCCCCCGCA GGTGGGAGCG GAGGTGAGGC AGAAACATGT CAGTTCACAA 
AGCCCCAAGC TGTTAAGAAT GCCCCATGGC AAACAGTCCA TCATCAGAGA CGCTCAAAGG 
AAAAGAGGGG AATTCCAGAA GAAGATACTA CTGCAATCCA ACACATCGGT GGAGAAGATA 
ATCTTCCTGC AGACCAGGCC TCGCCTGCCA CACATAGCTG CCCTGCTGAG CAGCTGCATC 
GATGGCTACA TCTATGCCTG GTCCATCCAC GGGAGCGGAG GCCTGTTGGG GAAGTTCCCT 
GTGGACTTCA AAAACCAGGG GGATGTGGGT GTGGGTGCCA TGGCCACGGA TGAGAAAGAC 
TTGATCCTTG TCACAGGAGA TTGTAAAGGG CACATCAAGA TTTGGGACAT CGAGGATTAT 
TGCATGTTCA CCGACAAGAC ACCACTTCAA CCCAGAGGGA CAAACAAGTT CCAATTCTTA 
ATTCCTGAGC AGGTCCAGAT CAACCTGCCA CACTACATCC CCTTGAAGGA GAAGGAGGTC 
ATGGCTGGCC AGACCATTTC CCTGGTTCCC CCCAAGCTTC TGAATGCCTG GAAGGGCCAT 
TTGGATAGTG TGGCAGACAT CCTGTATGTG GATAGCTTCC AGCTGATTAT CAGTGCTGGC 
CACGATTGTG ACGTCAAGGC TTGGAAACTC TCCGGTGACA CCATTGGGAC GTTTGGCGTG 
AGTGTTTGGA AAGGGTTGCA GGATATTCAG ACGGTTGGTG ATCTTGCACT AAGAAAAAGC 
TCAGAGGAGG AGGCTGACTC TATAGATGCT GCCCAGAAGG CCTTCCGTCC AGAGCTGCAG 
GAGGAGCGGG CTCTTGCCGA GGCCCTGGTG TACCAGCGGC GGGAGCAGGT GGCACTCATG 
GCTCTCCTGA ATGGGAAGGC AGACGCGGAG GCTGAGGCTT GGGGTAAGCT GCAGAGGATA 
ACCCAGATGT CCCCATGGGC TGGAGAGCAC TACTCAGAAG ACGTTGAGCA CAGCTGGCTC 
GAGTGGGAGT CCAAGGGCAA GCAGGTGAGC AAAATCGTGG GAGGAGCATA CAAGGCCAAG 
GACCGGTCTC GGAGTCCCGG AGTCCTGTCC ACTAATGTGC AGTATGGCTG GATGAAGCAC 
CAGATTTCAC CTCAGATCTA CCAAAGCCTG CACTTCAACC AGCTGTCACC CACCCACACA 
CCTGACTTCC TGGTGCACAA TATCCTAGGG CATCACGGCC AGCACATCTA CCTGGTGACC 
CACAATAGCC AAAAGGACCT GGATCCCGAA GAGACCAACT CGTCTACTGC CTCTGTCTCC 
CCCAGCGACA CCCTCACCCC GAAAGCCTCA GGCTCCGGGT TCCTGGAGTC CGCCTTGTCC 
ACCTCCCCGT ACCTCCAGCC CTCCCTCTCC CTCCTGTCCC AGCCATCCAG GTATGTCACG 
CAGAGGTCGG CGCCCCCCAT GCCTGTAGCC TCATCTATCA AACTGCTCTC AAGACCTCTG 
AAGGCCGCCC TTTCATCCTC CATGAAGAAG AGCCACGGCG TCAGGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_022408909.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022264617.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X10, mRNA.

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CloneID	OCe296638
Clone ID Related Accession (Same CDS sequence)	XM_022408909.1
Accession Version	XM_022408909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGGTGGGA GCGGAGGGTA GGGAGACACT CCCTCACCAT 
GATGATCTGG GAGGCCCATT TTTCTTTCCA GAAACATGTC AGTTCACAAA GCCCCAAGCT 
GTTAAGAATG CCCCATGGCA AACAGTCCAT CATCAGAGAC GCTCAAAGGA AAAGAGGGGA 
ATTCCAGAAG AAGATACTAC TGCAATCCAA CACATCGGTG GAGAAGGGAC GTTTGGCGTG 
AGTGTTTGGA AAGGGTTGCA GGATATTCAG ACGGTTGGTG ATCTTGCACT AAGAAAAAGC 
TCAGAGGAGG AGGCTGACTC TATAGATGCT GCCCAGAAGG CCTTCCGTCC AGAGCTGCAG 
GAGGAGCGGG CTCTTGCCGA GGCCCTGGTG TACCAGCGGC GGGAGCAGGT GGCACTCATG 
GCTCTCCTGA ATGGGAAGGC AGACGCGGAG GCTGAGGCTT GGGGTAAGCT GCAGAGGATA 
ACCCAGATGT CCCCATGGGC TGGAGAGCAC TACTCAGAAG ACGTTGAGCA CAGCTGGCTC 
GAGTGGGAGT CCAAGGGCAA GCAGGTGAGC AAAATCGTGG GAGGAGCATA CAAGGCCAAG 
GACCGGTCTC GGAGTCCCGG AGTCCTGTCC ACTAATGTGC AGTATGGCTG GATGAAGCAC 
CAGATTTCAC CTCAGATCTA CCAAAGCCTG CACTTCAACC AGCTGTCACC CACCCACACA 
CCTGACTTCC TGGTGCACAA TATCCTAGGG CATCACGGCC AGCACATCTA CCTGGTGACC 
CACAATAGCC AAAAGGACCT GGATCCCGAA GAGACCAACT CGTCTACTGC CTCTGTCTCC 
CCCAGCGACA CCCTCACCCC GAAAGCCTCA GGCTCCGGGT TCCTGGAGTC CGCCTTGTCC 
ACCTCCCCGT ACCTCCAGCC CTCCCTCTCC CTCCTGTCCC AGCCATCCAG GTATGTCACG 
CAGAGGTCGG CGCCCCCCAT GCCTGTAGCC TCATCTATCA AACTGCTCTC AAGACCTCTG 
AAGGCCGCCC TTTCATCCTC CATGAAGAAG AGCCACGGCG TCAGGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264617.1
CDS	1140..4550
Translation	MSQLRWKSKTNIFLSKIASKVTWEEQLGSGSKPSSSEDLRESPQSQEASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGALDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEGRETLPHHDDLGGPFFFPETCQFTKPQAVKNAPWQTVHHQRRSKEKRGIPEEDTTAIQHIGGEGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_022408909.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408909.1

ORF Insert Sequence:

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGGTGGGA GCGGAGGGTA GGGAGACACT CCCTCACCAT 
GATGATCTGG GAGGCCCATT TTTCTTTCCA GAAACATGTC AGTTCACAAA GCCCCAAGCT 
GTTAAGAATG CCCCATGGCA AACAGTCCAT CATCAGAGAC GCTCAAAGGA AAAGAGGGGA 
ATTCCAGAAG AAGATACTAC TGCAATCCAA CACATCGGTG GAGAAGGGAC GTTTGGCGTG 
AGTGTTTGGA AAGGGTTGCA GGATATTCAG ACGGTTGGTG ATCTTGCACT AAGAAAAAGC 
TCAGAGGAGG AGGCTGACTC TATAGATGCT GCCCAGAAGG CCTTCCGTCC AGAGCTGCAG 
GAGGAGCGGG CTCTTGCCGA GGCCCTGGTG TACCAGCGGC GGGAGCAGGT GGCACTCATG 
GCTCTCCTGA ATGGGAAGGC AGACGCGGAG GCTGAGGCTT GGGGTAAGCT GCAGAGGATA 
ACCCAGATGT CCCCATGGGC TGGAGAGCAC TACTCAGAAG ACGTTGAGCA CAGCTGGCTC 
GAGTGGGAGT CCAAGGGCAA GCAGGTGAGC AAAATCGTGG GAGGAGCATA CAAGGCCAAG 
GACCGGTCTC GGAGTCCCGG AGTCCTGTCC ACTAATGTGC AGTATGGCTG GATGAAGCAC 
CAGATTTCAC CTCAGATCTA CCAAAGCCTG CACTTCAACC AGCTGTCACC CACCCACACA 
CCTGACTTCC TGGTGCACAA TATCCTAGGG CATCACGGCC AGCACATCTA CCTGGTGACC 
CACAATAGCC AAAAGGACCT GGATCCCGAA GAGACCAACT CGTCTACTGC CTCTGTCTCC 
CCCAGCGACA CCCTCACCCC GAAAGCCTCA GGCTCCGGGT TCCTGGAGTC CGCCTTGTCC 
ACCTCCCCGT ACCTCCAGCC CTCCCTCTCC CTCCTGTCCC AGCCATCCAG GTATGTCACG 
CAGAGGTCGG CGCCCCCCAT GCCTGTAGCC TCATCTATCA AACTGCTCTC AAGACCTCTG 
AAGGCCGCCC TTTCATCCTC CATGAAGAAG AGCCACGGCG TCAGGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_022408911.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022264619.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCe296639
Clone ID Related Accession (Same CDS sequence)	XM_022408911.1 , XM_022408910.1
Accession Version	XM_022408911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCACC TTCTGACTGT CACCAAGGAC GGGATCTTGC AGATCTGGTC TGAATCCTTC 
TCGCTGCTTA ACTCCTTTAG GCTTAACCAG ATCCATCAGC CCTATAACCA GCAGATGTGG 
GTCACTGATA TGGTGTGTCT CCATAACATG AACCTGATTG CGATTGCATC AACTGACCAG 
AAAATAGAGT TCTTTGATAT TACCAACCAT AATTGTGCCC GGGCCTTCAC CTTCATTGAT 
CTGGACAGCT GTGTCCTGAC CATGGACTAT TGGTCCGACT ATCACAGAGG TGTATTCTGC 
TACGGGGACA CCAAAGGCAA TGTCATTGTC TTCACCTCTG ACAATGTGAC CCACGGGCTG 
TTCAACCCTC GAATCCTTCC CAGGACCTCC AAATGGGATC ACTGGACCAA AGTTTCTACA 
CGGAAGCTTC TAAATGAGAA GTCTCCTTTG TACAGGAGTT ATTGGCTAAA GGCTCTCCAT 
CCCAACTGGT GCCAACAGGT CAAGTTCATT CCTCAGCTGA ACACAGTGGC CTCCTGTTCG 
GCCATTGAGA AGTCCTCTCT GGTGCTAATA GCATTGCCAT CTAAAGCCCC AGAGAGCCCC 
AAGTTTTCAG TGCTGAACTT AAGAAAAGGG ATTCTTTGCT TCGATTACTG CCCAGACAAG 
AACTTAGTGG TGACTGGTGG CTATGACCCC CTCATCCGCC TGTGGAACCC CTTCTTCTCA 
AAAAAGCCTG TGTGGATGAT GAAGGGGCAT CAGACTTCCG TGACGCACAT CCTCGTGAAC 
AGCAAGAACA GCAGCATCCT CCTCAGCATC TCCAAGGACA AGAATATCCG TGTGTGGGAC 
ATGCAGGACT ACATATGCCT CCAGTCCTTT TCCGGGAAGC TTTTTGCCCT GGGAAACTGC 
CCCATCACCA GTGTCTACTT CCACAAGAAT GAAGACAGTC TCATCTGTAG CACCTTTTCA 
ATTGGGATCC TAAAAGGGTA CTTGGAGACT GAGGGGCCTG GGAAACCAAG GGAGAGGACC 
ACAACTTATA GCACACCCCT GTGCGCTGTT CTCTACAGCA AGATTTTTAA GCAGGTGGTA 
AGCGGCTGCC TGAGCAGCTT GGTGAGTGTG TGGGAGATCA AGACAGGCAA GAGGATGATG 
GAGTTCTCTG TGACTGGCGA CCAGCAAGTG GAGCTGACCA CCATGTGCCT GGATGAATCC 
GAGCGGTGCC TGCTCACGGG TTTGCGCGAC GGCACCATAA AGATGTGGAA CTACACTGTG 
GGTGAATGCC TGCTGACCTT CCCCAACCCG GACCAGCTGG AGATTAGCGG GATTGTCCAT 
ATGAACAAAG TGTTCTACGT GACTGGATGG AGTAAGAGAA TCACCTGTTT CATGCTCCAT 
AAGACCAAGC CAGTCCTCCT GTGCTACCAA TGGCAGAGCT TCCACAAGGA AGATGTTCTG 
AGCATGGCCA AGTACCAAAA CCAGTTCCTT GGGACCTCTT CCTACAATGG AGATATTCTC 
TTCTGGAACA TCAACCTGTT CAAGCCCATC CTCAATTTCA ATGCTTCTAA GAGCCCCTGG 
CCTTTGCTGC CCAAGAAGAT CCAAGAAGTG GACAACTCCC TGGCTGAGAG CCTCCAGTCC 
AGCAAGCTTT GTGTGAAACA TAAGGAGTGG GCGTACAAAC CATCAACGCA GCCCCTGGGC 
CCCAGAGCCA GGGCTGTAGC CAATGCCAAC CTGCAACGGA ACCTGATGTC AGCTCCCCCA 
GTGATGAAGC GCCCCAGAGA CAAGGAGCCA GAAAAGCCCA TGCCCCCGCA GGTGGGAGCG 
GAGGTGAGGC AGAAACATGT CAGTTCACAA AGCCCCAAGC TGTTAAGAAT GCCCCATGGC 
AAACAGTCCA TCATCAGAGA CGCTCAAAGG AAAAGAGGGG AATTCCAGAA GAAGATACTA 
CTGCAATCCA ACACATCGGT GGAGAAGATA ATCTTCCTGC AGACCAGGCC TCGCCTGCCA 
CACATAGCTG CCCTGCTGAG CAGCTGCATC GATGGCTACA TCTATGCCTG GTCCATCCAC 
GGGAGCGGAG GCCTGTTGGG GAAGTTCCCT GTGGACTTCA AAAACCAGGG GGATGTGGGT 
GTGGGTGCCA TGGCCACGGA TGAGAAAGAC TTGATCCTTG TCACAGGAGA TTGTAAAGGG 
CACATCAAGA TTTGGGACAT CGAGGATTAT TGCATGTTCA CCGACAAGAC ACCACTTCAA 
CCCAGAGGGA CAAACAAGTT CCAATTCTTA ATTCCTGAGC AGGTCCAGAT CAACCTGCCA 
CACTACATCC CCTTGAAGGA GAAGGAGGTC ATGGCTGGCC AGACCATTTC CCTGGTTCCC 
CCCAAGCTTC TGAATGCCTG GAAGGGCCAT TTGGATAGTG TGGCAGACAT CCTGTATGTG 
GATAGCTTCC AGCTGATTAT CAGTGCTGGC CACGATTGTG ACGTCAAGGC TTGGAAACTC 
TCCGGTGACA CCATTGGGAC GTTTGGCGTG AGTGTTTGGA AAGGGTTGCA GGATATTCAG 
ACGGTTGGTG ATCTTGCACT AAGAAAAAGC TCAGAGGAGG AGGCTGACTC TATAGATGCT 
GCCCAGAAGG CCTTCCGTCC AGAGCTGCAG GAGGAGCGGG CTCTTGCCGA GGCCCTGGTG 
TACCAGCGGC GGGAGCAGGT GGCACTCATG GCTCTCCTGA ATGGGAAGGC AGACGCGGAG 
GCTGAGGCTT GGGGTAAGCT GCAGAGGATA ACCCAGATGT CCCCATGGGC TGGAGAGCAC 
TACTCAGAAG ACGTTGAGCA CAGCTGGCTC GAGTGGGAGT CCAAGGGCAA GCAGGTGAGC 
AAAATCGTGG GAGGAGCATA CAAGGCCAAG GACCGGTCTC GGAGTCCCGG AGTCCTGTCC 
ACTAATGTGC AGTATGGCTG GATGAAGCAC CAGATTTCAC CTCAGATCTA CCAAAGCCTG 
CACTTCAACC AGCTGTCACC CACCCACACA CCTGACTTCC TGGTGCACAA TATCCTAGGG 
CATCACGGCC AGCACATCTA CCTGGTGACC CACAATAGCC AAAAGGACCT GGATCCCGAA 
GAGACCAACT CGTCTACTGC CTCTGTCTCC CCCAGCGACA CCCTCACCCC GAAAGCCTCA 
GGCTCCGGGT TCCTGGAGTC CGCCTTGTCC ACCTCCCCGT ACCTCCAGCC CTCCCTCTCC 
CTCCTGTCCC AGCCATCCAG GTATGTCACG CAGAGGTCGG CGCCCCCCAT GCCTGTAGCC 
TCATCTATCA AACTGCTCTC AAGACCTCTG AAGGCCGCCC TTTCATCCTC CATGAAGAAG 
AGCCACGGCG TCAGGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264619.1
CDS	180..3500
Translation	MGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_022408911.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408911.1

ORF Insert Sequence:

ATGGGGCACC TTCTGACTGT CACCAAGGAC GGGATCTTGC AGATCTGGTC TGAATCCTTC 
TCGCTGCTTA ACTCCTTTAG GCTTAACCAG ATCCATCAGC CCTATAACCA GCAGATGTGG 
GTCACTGATA TGGTGTGTCT CCATAACATG AACCTGATTG CGATTGCATC AACTGACCAG 
AAAATAGAGT TCTTTGATAT TACCAACCAT AATTGTGCCC GGGCCTTCAC CTTCATTGAT 
CTGGACAGCT GTGTCCTGAC CATGGACTAT TGGTCCGACT ATCACAGAGG TGTATTCTGC 
TACGGGGACA CCAAAGGCAA TGTCATTGTC TTCACCTCTG ACAATGTGAC CCACGGGCTG 
TTCAACCCTC GAATCCTTCC CAGGACCTCC AAATGGGATC ACTGGACCAA AGTTTCTACA 
CGGAAGCTTC TAAATGAGAA GTCTCCTTTG TACAGGAGTT ATTGGCTAAA GGCTCTCCAT 
CCCAACTGGT GCCAACAGGT CAAGTTCATT CCTCAGCTGA ACACAGTGGC CTCCTGTTCG 
GCCATTGAGA AGTCCTCTCT GGTGCTAATA GCATTGCCAT CTAAAGCCCC AGAGAGCCCC 
AAGTTTTCAG TGCTGAACTT AAGAAAAGGG ATTCTTTGCT TCGATTACTG CCCAGACAAG 
AACTTAGTGG TGACTGGTGG CTATGACCCC CTCATCCGCC TGTGGAACCC CTTCTTCTCA 
AAAAAGCCTG TGTGGATGAT GAAGGGGCAT CAGACTTCCG TGACGCACAT CCTCGTGAAC 
AGCAAGAACA GCAGCATCCT CCTCAGCATC TCCAAGGACA AGAATATCCG TGTGTGGGAC 
ATGCAGGACT ACATATGCCT CCAGTCCTTT TCCGGGAAGC TTTTTGCCCT GGGAAACTGC 
CCCATCACCA GTGTCTACTT CCACAAGAAT GAAGACAGTC TCATCTGTAG CACCTTTTCA 
ATTGGGATCC TAAAAGGGTA CTTGGAGACT GAGGGGCCTG GGAAACCAAG GGAGAGGACC 
ACAACTTATA GCACACCCCT GTGCGCTGTT CTCTACAGCA AGATTTTTAA GCAGGTGGTA 
AGCGGCTGCC TGAGCAGCTT GGTGAGTGTG TGGGAGATCA AGACAGGCAA GAGGATGATG 
GAGTTCTCTG TGACTGGCGA CCAGCAAGTG GAGCTGACCA CCATGTGCCT GGATGAATCC 
GAGCGGTGCC TGCTCACGGG TTTGCGCGAC GGCACCATAA AGATGTGGAA CTACACTGTG 
GGTGAATGCC TGCTGACCTT CCCCAACCCG GACCAGCTGG AGATTAGCGG GATTGTCCAT 
ATGAACAAAG TGTTCTACGT GACTGGATGG AGTAAGAGAA TCACCTGTTT CATGCTCCAT 
AAGACCAAGC CAGTCCTCCT GTGCTACCAA TGGCAGAGCT TCCACAAGGA AGATGTTCTG 
AGCATGGCCA AGTACCAAAA CCAGTTCCTT GGGACCTCTT CCTACAATGG AGATATTCTC 
TTCTGGAACA TCAACCTGTT CAAGCCCATC CTCAATTTCA ATGCTTCTAA GAGCCCCTGG 
CCTTTGCTGC CCAAGAAGAT CCAAGAAGTG GACAACTCCC TGGCTGAGAG CCTCCAGTCC 
AGCAAGCTTT GTGTGAAACA TAAGGAGTGG GCGTACAAAC CATCAACGCA GCCCCTGGGC 
CCCAGAGCCA GGGCTGTAGC CAATGCCAAC CTGCAACGGA ACCTGATGTC AGCTCCCCCA 
GTGATGAAGC GCCCCAGAGA CAAGGAGCCA GAAAAGCCCA TGCCCCCGCA GGTGGGAGCG 
GAGGTGAGGC AGAAACATGT CAGTTCACAA AGCCCCAAGC TGTTAAGAAT GCCCCATGGC 
AAACAGTCCA TCATCAGAGA CGCTCAAAGG AAAAGAGGGG AATTCCAGAA GAAGATACTA 
CTGCAATCCA ACACATCGGT GGAGAAGATA ATCTTCCTGC AGACCAGGCC TCGCCTGCCA 
CACATAGCTG CCCTGCTGAG CAGCTGCATC GATGGCTACA TCTATGCCTG GTCCATCCAC 
GGGAGCGGAG GCCTGTTGGG GAAGTTCCCT GTGGACTTCA AAAACCAGGG GGATGTGGGT 
GTGGGTGCCA TGGCCACGGA TGAGAAAGAC TTGATCCTTG TCACAGGAGA TTGTAAAGGG 
CACATCAAGA TTTGGGACAT CGAGGATTAT TGCATGTTCA CCGACAAGAC ACCACTTCAA 
CCCAGAGGGA CAAACAAGTT CCAATTCTTA ATTCCTGAGC AGGTCCAGAT CAACCTGCCA 
CACTACATCC CCTTGAAGGA GAAGGAGGTC ATGGCTGGCC AGACCATTTC CCTGGTTCCC 
CCCAAGCTTC TGAATGCCTG GAAGGGCCAT TTGGATAGTG TGGCAGACAT CCTGTATGTG 
GATAGCTTCC AGCTGATTAT CAGTGCTGGC CACGATTGTG ACGTCAAGGC TTGGAAACTC 
TCCGGTGACA CCATTGGGAC GTTTGGCGTG AGTGTTTGGA AAGGGTTGCA GGATATTCAG 
ACGGTTGGTG ATCTTGCACT AAGAAAAAGC TCAGAGGAGG AGGCTGACTC TATAGATGCT 
GCCCAGAAGG CCTTCCGTCC AGAGCTGCAG GAGGAGCGGG CTCTTGCCGA GGCCCTGGTG 
TACCAGCGGC GGGAGCAGGT GGCACTCATG GCTCTCCTGA ATGGGAAGGC AGACGCGGAG 
GCTGAGGCTT GGGGTAAGCT GCAGAGGATA ACCCAGATGT CCCCATGGGC TGGAGAGCAC 
TACTCAGAAG ACGTTGAGCA CAGCTGGCTC GAGTGGGAGT CCAAGGGCAA GCAGGTGAGC 
AAAATCGTGG GAGGAGCATA CAAGGCCAAG GACCGGTCTC GGAGTCCCGG AGTCCTGTCC 
ACTAATGTGC AGTATGGCTG GATGAAGCAC CAGATTTCAC CTCAGATCTA CCAAAGCCTG 
CACTTCAACC AGCTGTCACC CACCCACACA CCTGACTTCC TGGTGCACAA TATCCTAGGG 
CATCACGGCC AGCACATCTA CCTGGTGACC CACAATAGCC AAAAGGACCT GGATCCCGAA 
GAGACCAACT CGTCTACTGC CTCTGTCTCC CCCAGCGACA CCCTCACCCC GAAAGCCTCA 
GGCTCCGGGT TCCTGGAGTC CGCCTTGTCC ACCTCCCCGT ACCTCCAGCC CTCCCTCTCC 
CTCCTGTCCC AGCCATCCAG GTATGTCACG CAGAGGTCGG CGCCCCCCAT GCCTGTAGCC 
TCATCTATCA AACTGCTCTC AAGACCTCTG AAGGCCGCCC TTTCATCCTC CATGAAGAAG 
AGCCACGGCG TCAGGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_022408910.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022264618.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe296639
Clone ID Related Accession (Same CDS sequence)	XM_022408911.1 , XM_022408910.1
Accession Version	XM_022408910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCACC TTCTGACTGT CACCAAGGAC GGGATCTTGC AGATCTGGTC TGAATCCTTC 
TCGCTGCTTA ACTCCTTTAG GCTTAACCAG ATCCATCAGC CCTATAACCA GCAGATGTGG 
GTCACTGATA TGGTGTGTCT CCATAACATG AACCTGATTG CGATTGCATC AACTGACCAG 
AAAATAGAGT TCTTTGATAT TACCAACCAT AATTGTGCCC GGGCCTTCAC CTTCATTGAT 
CTGGACAGCT GTGTCCTGAC CATGGACTAT TGGTCCGACT ATCACAGAGG TGTATTCTGC 
TACGGGGACA CCAAAGGCAA TGTCATTGTC TTCACCTCTG ACAATGTGAC CCACGGGCTG 
TTCAACCCTC GAATCCTTCC CAGGACCTCC AAATGGGATC ACTGGACCAA AGTTTCTACA 
CGGAAGCTTC TAAATGAGAA GTCTCCTTTG TACAGGAGTT ATTGGCTAAA GGCTCTCCAT 
CCCAACTGGT GCCAACAGGT CAAGTTCATT CCTCAGCTGA ACACAGTGGC CTCCTGTTCG 
GCCATTGAGA AGTCCTCTCT GGTGCTAATA GCATTGCCAT CTAAAGCCCC AGAGAGCCCC 
AAGTTTTCAG TGCTGAACTT AAGAAAAGGG ATTCTTTGCT TCGATTACTG CCCAGACAAG 
AACTTAGTGG TGACTGGTGG CTATGACCCC CTCATCCGCC TGTGGAACCC CTTCTTCTCA 
AAAAAGCCTG TGTGGATGAT GAAGGGGCAT CAGACTTCCG TGACGCACAT CCTCGTGAAC 
AGCAAGAACA GCAGCATCCT CCTCAGCATC TCCAAGGACA AGAATATCCG TGTGTGGGAC 
ATGCAGGACT ACATATGCCT CCAGTCCTTT TCCGGGAAGC TTTTTGCCCT GGGAAACTGC 
CCCATCACCA GTGTCTACTT CCACAAGAAT GAAGACAGTC TCATCTGTAG CACCTTTTCA 
ATTGGGATCC TAAAAGGGTA CTTGGAGACT GAGGGGCCTG GGAAACCAAG GGAGAGGACC 
ACAACTTATA GCACACCCCT GTGCGCTGTT CTCTACAGCA AGATTTTTAA GCAGGTGGTA 
AGCGGCTGCC TGAGCAGCTT GGTGAGTGTG TGGGAGATCA AGACAGGCAA GAGGATGATG 
GAGTTCTCTG TGACTGGCGA CCAGCAAGTG GAGCTGACCA CCATGTGCCT GGATGAATCC 
GAGCGGTGCC TGCTCACGGG TTTGCGCGAC GGCACCATAA AGATGTGGAA CTACACTGTG 
GGTGAATGCC TGCTGACCTT CCCCAACCCG GACCAGCTGG AGATTAGCGG GATTGTCCAT 
ATGAACAAAG TGTTCTACGT GACTGGATGG AGTAAGAGAA TCACCTGTTT CATGCTCCAT 
AAGACCAAGC CAGTCCTCCT GTGCTACCAA TGGCAGAGCT TCCACAAGGA AGATGTTCTG 
AGCATGGCCA AGTACCAAAA CCAGTTCCTT GGGACCTCTT CCTACAATGG AGATATTCTC 
TTCTGGAACA TCAACCTGTT CAAGCCCATC CTCAATTTCA ATGCTTCTAA GAGCCCCTGG 
CCTTTGCTGC CCAAGAAGAT CCAAGAAGTG GACAACTCCC TGGCTGAGAG CCTCCAGTCC 
AGCAAGCTTT GTGTGAAACA TAAGGAGTGG GCGTACAAAC CATCAACGCA GCCCCTGGGC 
CCCAGAGCCA GGGCTGTAGC CAATGCCAAC CTGCAACGGA ACCTGATGTC AGCTCCCCCA 
GTGATGAAGC GCCCCAGAGA CAAGGAGCCA GAAAAGCCCA TGCCCCCGCA GGTGGGAGCG 
GAGGTGAGGC AGAAACATGT CAGTTCACAA AGCCCCAAGC TGTTAAGAAT GCCCCATGGC 
AAACAGTCCA TCATCAGAGA CGCTCAAAGG AAAAGAGGGG AATTCCAGAA GAAGATACTA 
CTGCAATCCA ACACATCGGT GGAGAAGATA ATCTTCCTGC AGACCAGGCC TCGCCTGCCA 
CACATAGCTG CCCTGCTGAG CAGCTGCATC GATGGCTACA TCTATGCCTG GTCCATCCAC 
GGGAGCGGAG GCCTGTTGGG GAAGTTCCCT GTGGACTTCA AAAACCAGGG GGATGTGGGT 
GTGGGTGCCA TGGCCACGGA TGAGAAAGAC TTGATCCTTG TCACAGGAGA TTGTAAAGGG 
CACATCAAGA TTTGGGACAT CGAGGATTAT TGCATGTTCA CCGACAAGAC ACCACTTCAA 
CCCAGAGGGA CAAACAAGTT CCAATTCTTA ATTCCTGAGC AGGTCCAGAT CAACCTGCCA 
CACTACATCC CCTTGAAGGA GAAGGAGGTC ATGGCTGGCC AGACCATTTC CCTGGTTCCC 
CCCAAGCTTC TGAATGCCTG GAAGGGCCAT TTGGATAGTG TGGCAGACAT CCTGTATGTG 
GATAGCTTCC AGCTGATTAT CAGTGCTGGC CACGATTGTG ACGTCAAGGC TTGGAAACTC 
TCCGGTGACA CCATTGGGAC GTTTGGCGTG AGTGTTTGGA AAGGGTTGCA GGATATTCAG 
ACGGTTGGTG ATCTTGCACT AAGAAAAAGC TCAGAGGAGG AGGCTGACTC TATAGATGCT 
GCCCAGAAGG CCTTCCGTCC AGAGCTGCAG GAGGAGCGGG CTCTTGCCGA GGCCCTGGTG 
TACCAGCGGC GGGAGCAGGT GGCACTCATG GCTCTCCTGA ATGGGAAGGC AGACGCGGAG 
GCTGAGGCTT GGGGTAAGCT GCAGAGGATA ACCCAGATGT CCCCATGGGC TGGAGAGCAC 
TACTCAGAAG ACGTTGAGCA CAGCTGGCTC GAGTGGGAGT CCAAGGGCAA GCAGGTGAGC 
AAAATCGTGG GAGGAGCATA CAAGGCCAAG GACCGGTCTC GGAGTCCCGG AGTCCTGTCC 
ACTAATGTGC AGTATGGCTG GATGAAGCAC CAGATTTCAC CTCAGATCTA CCAAAGCCTG 
CACTTCAACC AGCTGTCACC CACCCACACA CCTGACTTCC TGGTGCACAA TATCCTAGGG 
CATCACGGCC AGCACATCTA CCTGGTGACC CACAATAGCC AAAAGGACCT GGATCCCGAA 
GAGACCAACT CGTCTACTGC CTCTGTCTCC CCCAGCGACA CCCTCACCCC GAAAGCCTCA 
GGCTCCGGGT TCCTGGAGTC CGCCTTGTCC ACCTCCCCGT ACCTCCAGCC CTCCCTCTCC 
CTCCTGTCCC AGCCATCCAG GTATGTCACG CAGAGGTCGG CGCCCCCCAT GCCTGTAGCC 
TCATCTATCA AACTGCTCTC AAGACCTCTG AAGGCCGCCC TTTCATCCTC CATGAAGAAG 
AGCCACGGCG TCAGGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264618.1
CDS	192..3512
Translation	MGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_022408910.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408910.1

ORF Insert Sequence:

ATGGGGCACC TTCTGACTGT CACCAAGGAC GGGATCTTGC AGATCTGGTC TGAATCCTTC 
TCGCTGCTTA ACTCCTTTAG GCTTAACCAG ATCCATCAGC CCTATAACCA GCAGATGTGG 
GTCACTGATA TGGTGTGTCT CCATAACATG AACCTGATTG CGATTGCATC AACTGACCAG 
AAAATAGAGT TCTTTGATAT TACCAACCAT AATTGTGCCC GGGCCTTCAC CTTCATTGAT 
CTGGACAGCT GTGTCCTGAC CATGGACTAT TGGTCCGACT ATCACAGAGG TGTATTCTGC 
TACGGGGACA CCAAAGGCAA TGTCATTGTC TTCACCTCTG ACAATGTGAC CCACGGGCTG 
TTCAACCCTC GAATCCTTCC CAGGACCTCC AAATGGGATC ACTGGACCAA AGTTTCTACA 
CGGAAGCTTC TAAATGAGAA GTCTCCTTTG TACAGGAGTT ATTGGCTAAA GGCTCTCCAT 
CCCAACTGGT GCCAACAGGT CAAGTTCATT CCTCAGCTGA ACACAGTGGC CTCCTGTTCG 
GCCATTGAGA AGTCCTCTCT GGTGCTAATA GCATTGCCAT CTAAAGCCCC AGAGAGCCCC 
AAGTTTTCAG TGCTGAACTT AAGAAAAGGG ATTCTTTGCT TCGATTACTG CCCAGACAAG 
AACTTAGTGG TGACTGGTGG CTATGACCCC CTCATCCGCC TGTGGAACCC CTTCTTCTCA 
AAAAAGCCTG TGTGGATGAT GAAGGGGCAT CAGACTTCCG TGACGCACAT CCTCGTGAAC 
AGCAAGAACA GCAGCATCCT CCTCAGCATC TCCAAGGACA AGAATATCCG TGTGTGGGAC 
ATGCAGGACT ACATATGCCT CCAGTCCTTT TCCGGGAAGC TTTTTGCCCT GGGAAACTGC 
CCCATCACCA GTGTCTACTT CCACAAGAAT GAAGACAGTC TCATCTGTAG CACCTTTTCA 
ATTGGGATCC TAAAAGGGTA CTTGGAGACT GAGGGGCCTG GGAAACCAAG GGAGAGGACC 
ACAACTTATA GCACACCCCT GTGCGCTGTT CTCTACAGCA AGATTTTTAA GCAGGTGGTA 
AGCGGCTGCC TGAGCAGCTT GGTGAGTGTG TGGGAGATCA AGACAGGCAA GAGGATGATG 
GAGTTCTCTG TGACTGGCGA CCAGCAAGTG GAGCTGACCA CCATGTGCCT GGATGAATCC 
GAGCGGTGCC TGCTCACGGG TTTGCGCGAC GGCACCATAA AGATGTGGAA CTACACTGTG 
GGTGAATGCC TGCTGACCTT CCCCAACCCG GACCAGCTGG AGATTAGCGG GATTGTCCAT 
ATGAACAAAG TGTTCTACGT GACTGGATGG AGTAAGAGAA TCACCTGTTT CATGCTCCAT 
AAGACCAAGC CAGTCCTCCT GTGCTACCAA TGGCAGAGCT TCCACAAGGA AGATGTTCTG 
AGCATGGCCA AGTACCAAAA CCAGTTCCTT GGGACCTCTT CCTACAATGG AGATATTCTC 
TTCTGGAACA TCAACCTGTT CAAGCCCATC CTCAATTTCA ATGCTTCTAA GAGCCCCTGG 
CCTTTGCTGC CCAAGAAGAT CCAAGAAGTG GACAACTCCC TGGCTGAGAG CCTCCAGTCC 
AGCAAGCTTT GTGTGAAACA TAAGGAGTGG GCGTACAAAC CATCAACGCA GCCCCTGGGC 
CCCAGAGCCA GGGCTGTAGC CAATGCCAAC CTGCAACGGA ACCTGATGTC AGCTCCCCCA 
GTGATGAAGC GCCCCAGAGA CAAGGAGCCA GAAAAGCCCA TGCCCCCGCA GGTGGGAGCG 
GAGGTGAGGC AGAAACATGT CAGTTCACAA AGCCCCAAGC TGTTAAGAAT GCCCCATGGC 
AAACAGTCCA TCATCAGAGA CGCTCAAAGG AAAAGAGGGG AATTCCAGAA GAAGATACTA 
CTGCAATCCA ACACATCGGT GGAGAAGATA ATCTTCCTGC AGACCAGGCC TCGCCTGCCA 
CACATAGCTG CCCTGCTGAG CAGCTGCATC GATGGCTACA TCTATGCCTG GTCCATCCAC 
GGGAGCGGAG GCCTGTTGGG GAAGTTCCCT GTGGACTTCA AAAACCAGGG GGATGTGGGT 
GTGGGTGCCA TGGCCACGGA TGAGAAAGAC TTGATCCTTG TCACAGGAGA TTGTAAAGGG 
CACATCAAGA TTTGGGACAT CGAGGATTAT TGCATGTTCA CCGACAAGAC ACCACTTCAA 
CCCAGAGGGA CAAACAAGTT CCAATTCTTA ATTCCTGAGC AGGTCCAGAT CAACCTGCCA 
CACTACATCC CCTTGAAGGA GAAGGAGGTC ATGGCTGGCC AGACCATTTC CCTGGTTCCC 
CCCAAGCTTC TGAATGCCTG GAAGGGCCAT TTGGATAGTG TGGCAGACAT CCTGTATGTG 
GATAGCTTCC AGCTGATTAT CAGTGCTGGC CACGATTGTG ACGTCAAGGC TTGGAAACTC 
TCCGGTGACA CCATTGGGAC GTTTGGCGTG AGTGTTTGGA AAGGGTTGCA GGATATTCAG 
ACGGTTGGTG ATCTTGCACT AAGAAAAAGC TCAGAGGAGG AGGCTGACTC TATAGATGCT 
GCCCAGAAGG CCTTCCGTCC AGAGCTGCAG GAGGAGCGGG CTCTTGCCGA GGCCCTGGTG 
TACCAGCGGC GGGAGCAGGT GGCACTCATG GCTCTCCTGA ATGGGAAGGC AGACGCGGAG 
GCTGAGGCTT GGGGTAAGCT GCAGAGGATA ACCCAGATGT CCCCATGGGC TGGAGAGCAC 
TACTCAGAAG ACGTTGAGCA CAGCTGGCTC GAGTGGGAGT CCAAGGGCAA GCAGGTGAGC 
AAAATCGTGG GAGGAGCATA CAAGGCCAAG GACCGGTCTC GGAGTCCCGG AGTCCTGTCC 
ACTAATGTGC AGTATGGCTG GATGAAGCAC CAGATTTCAC CTCAGATCTA CCAAAGCCTG 
CACTTCAACC AGCTGTCACC CACCCACACA CCTGACTTCC TGGTGCACAA TATCCTAGGG 
CATCACGGCC AGCACATCTA CCTGGTGACC CACAATAGCC AAAAGGACCT GGATCCCGAA 
GAGACCAACT CGTCTACTGC CTCTGTCTCC CCCAGCGACA CCCTCACCCC GAAAGCCTCA 
GGCTCCGGGT TCCTGGAGTC CGCCTTGTCC ACCTCCCCGT ACCTCCAGCC CTCCCTCTCC 
CTCCTGTCCC AGCCATCCAG GTATGTCACG CAGAGGTCGG CGCCCCCCAT GCCTGTAGCC 
TCATCTATCA AACTGCTCTC AAGACCTCTG AAGGCCGCCC TTTCATCCTC CATGAAGAAG 
AGCCACGGCG TCAGGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107219.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962694.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe42868
Clone ID Related Accession (Same CDS sequence)	XM_014107219.1 , XM_014107219.2
Accession Version	XM_014107219.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGGTGGGA GCGGAGGTGA GGCAGAAACA TGTCAGTTCA 
CAAAGCCCCA AGCTGTTAAG AATGCCCCAT GGCAAACAGT CCATCATCAG AGACGCTCAA 
AGGAAAAGAG GGGAATTCCA GAAGAAGATA CTACTGCAAT CCAACACATC GGTGGAGAAG 
ATAATCTTCC TGCAGACCAG GCCTCGCCTG CCACACATAG CTGCCCTGCT GAGCAGCTGC 
ATCGATGGCT ACATCTATGC CTGGTCCATC CACGGGAGCG GAGGCCTGTT GGGGAAGTTC 
CCTGTGGACT TCAAAAACCA GGGGGATGTG GGTGTGGGTG CCATGGCCAC GGATGAGAAA 
GACTTGATCC TTGTCACAGG AGATTGTAAA GGGCACATCA AGATTTGGGA CATCGAGGAT 
TATTGCATGT TCACCGACAA GACACCACTT CAACCCAGAG GGACAAACAA GTTCCAATTC 
TTAATTCCTG AGCAGGTCCA GATCAACCTG CCACACTACA TCCCCTTGAA GGAGAAGGAG 
GTCATGGCTG GCCAGACCAT TTCCCTGGTT CCCCCCAAGC TTCTGAATGC CTGGAAGGGC 
CATTTGGATA GTGTGGCAGA CATCCTGTAT GTGGATAGCT TCCAGCTGAT TATCAGTGCT 
GGCCACGATT GTGACGTCAA GGCTTGGAAA CTCTCCGGTG ACACCATTGG GACGTTTGGC 
GTGAGTGTTT GGAAAGGGTT GCAGGATATT CAGACGGTTG GTGATCTTGC ACTAAGAAAA 
AGCTCAGAGG AGGAGGCTGA CTCTATAGAT GCTGCCCAGA AGGCCTTCCG TCCAGAGCTG 
CAGGAGGAGC GGGCTCTTGC CGAGGCCCTG GTGTACCAGC GGCGGGAGCA GGTGGCACTC 
ATGGCTCTCC TGAATGGGAA GGCAGACGCG GAGGCTGAGG CTTGGGGTAA GCTGCAGAGG 
ATAACCCAGA TGTCCCCATG GGCTGGAGAG CACTACTCAG AAGACGTTGA GCACAGCTGG 
CTCGAGTGGG AGTCCAAGGG CAAGCAGGTG AGCAAAATCG TGGGAGGAGC ATACAAGGCC 
AAGGACCGGT CTCGGAGTCC CGGAGTCCTG TCCACTAATG TGCAGTATGG CTGGATGAAG 
CACCAGATTT CACCTCAGAT CTACCAAAGC CTGCACTTCA ACCAGCTGTC ACCCACCCAC 
ACACCTGACT TCCTGGTGCA CAATATCCTA GGGCATCACG GCCAGCACAT CTACCTGGTG 
ACCCACAATA GCCAAAAGGA CCTGGATCCC GAAGAGACCA ACTCGTCTAC TGCCTCTGTC 
TCCCCCAGCG ACACCCTCAC CCCGAAAGCC TCAGGCTCCG GGTTCCTGGA GTCCGCCTTG 
TCCACCTCCC CGTACCTCCA GCCCTCCCTC TCCCTCCTGT CCCAGCCATC CAGGTATGTC 
ACGCAGAGGT CGGCGCCCCC CATGCCTGTA GCCTCATCTA TCAAACTGCT CTCAAGACCT 
CTGAAGGCCG CCCTTTCATC CTCCATGAAG AAGAGCCACG GCGTCAGGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962694.1
CDS	1391..5284
Translation	MSQLRWKSKTNIFLSKIASKVTWEEQLGSGSKPSSSEDLRESPQSQEASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGALDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107219.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107219.1

ORF Insert Sequence:

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGGTGGGA GCGGAGGTGA GGCAGAAACA TGTCAGTTCA 
CAAAGCCCCA AGCTGTTAAG AATGCCCCAT GGCAAACAGT CCATCATCAG AGACGCTCAA 
AGGAAAAGAG GGGAATTCCA GAAGAAGATA CTACTGCAAT CCAACACATC GGTGGAGAAG 
ATAATCTTCC TGCAGACCAG GCCTCGCCTG CCACACATAG CTGCCCTGCT GAGCAGCTGC 
ATCGATGGCT ACATCTATGC CTGGTCCATC CACGGGAGCG GAGGCCTGTT GGGGAAGTTC 
CCTGTGGACT TCAAAAACCA GGGGGATGTG GGTGTGGGTG CCATGGCCAC GGATGAGAAA 
GACTTGATCC TTGTCACAGG AGATTGTAAA GGGCACATCA AGATTTGGGA CATCGAGGAT 
TATTGCATGT TCACCGACAA GACACCACTT CAACCCAGAG GGACAAACAA GTTCCAATTC 
TTAATTCCTG AGCAGGTCCA GATCAACCTG CCACACTACA TCCCCTTGAA GGAGAAGGAG 
GTCATGGCTG GCCAGACCAT TTCCCTGGTT CCCCCCAAGC TTCTGAATGC CTGGAAGGGC 
CATTTGGATA GTGTGGCAGA CATCCTGTAT GTGGATAGCT TCCAGCTGAT TATCAGTGCT 
GGCCACGATT GTGACGTCAA GGCTTGGAAA CTCTCCGGTG ACACCATTGG GACGTTTGGC 
GTGAGTGTTT GGAAAGGGTT GCAGGATATT CAGACGGTTG GTGATCTTGC ACTAAGAAAA 
AGCTCAGAGG AGGAGGCTGA CTCTATAGAT GCTGCCCAGA AGGCCTTCCG TCCAGAGCTG 
CAGGAGGAGC GGGCTCTTGC CGAGGCCCTG GTGTACCAGC GGCGGGAGCA GGTGGCACTC 
ATGGCTCTCC TGAATGGGAA GGCAGACGCG GAGGCTGAGG CTTGGGGTAA GCTGCAGAGG 
ATAACCCAGA TGTCCCCATG GGCTGGAGAG CACTACTCAG AAGACGTTGA GCACAGCTGG 
CTCGAGTGGG AGTCCAAGGG CAAGCAGGTG AGCAAAATCG TGGGAGGAGC ATACAAGGCC 
AAGGACCGGT CTCGGAGTCC CGGAGTCCTG TCCACTAATG TGCAGTATGG CTGGATGAAG 
CACCAGATTT CACCTCAGAT CTACCAAAGC CTGCACTTCA ACCAGCTGTC ACCCACCCAC 
ACACCTGACT TCCTGGTGCA CAATATCCTA GGGCATCACG GCCAGCACAT CTACCTGGTG 
ACCCACAATA GCCAAAAGGA CCTGGATCCC GAAGAGACCA ACTCGTCTAC TGCCTCTGTC 
TCCCCCAGCG ACACCCTCAC CCCGAAAGCC TCAGGCTCCG GGTTCCTGGA GTCCGCCTTG 
TCCACCTCCC CGTACCTCCA GCCCTCCCTC TCCCTCCTGT CCCAGCCATC CAGGTATGTC 
ACGCAGAGGT CGGCGCCCCC CATGCCTGTA GCCTCATCTA TCAAACTGCT CTCAAGACCT 
CTGAAGGCCG CCCTTTCATC CTCCATGAAG AAGAGCCACG GCGTCAGGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107219.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962694.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe42868
Clone ID Related Accession (Same CDS sequence)	XM_014107219.1 , XM_014107219.2
Accession Version	XM_014107219.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107219.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGGTGGGA GCGGAGGTGA GGCAGAAACA TGTCAGTTCA 
CAAAGCCCCA AGCTGTTAAG AATGCCCCAT GGCAAACAGT CCATCATCAG AGACGCTCAA 
AGGAAAAGAG GGGAATTCCA GAAGAAGATA CTACTGCAAT CCAACACATC GGTGGAGAAG 
ATAATCTTCC TGCAGACCAG GCCTCGCCTG CCACACATAG CTGCCCTGCT GAGCAGCTGC 
ATCGATGGCT ACATCTATGC CTGGTCCATC CACGGGAGCG GAGGCCTGTT GGGGAAGTTC 
CCTGTGGACT TCAAAAACCA GGGGGATGTG GGTGTGGGTG CCATGGCCAC GGATGAGAAA 
GACTTGATCC TTGTCACAGG AGATTGTAAA GGGCACATCA AGATTTGGGA CATCGAGGAT 
TATTGCATGT TCACCGACAA GACACCACTT CAACCCAGAG GGACAAACAA GTTCCAATTC 
TTAATTCCTG AGCAGGTCCA GATCAACCTG CCACACTACA TCCCCTTGAA GGAGAAGGAG 
GTCATGGCTG GCCAGACCAT TTCCCTGGTT CCCCCCAAGC TTCTGAATGC CTGGAAGGGC 
CATTTGGATA GTGTGGCAGA CATCCTGTAT GTGGATAGCT TCCAGCTGAT TATCAGTGCT 
GGCCACGATT GTGACGTCAA GGCTTGGAAA CTCTCCGGTG ACACCATTGG GACGTTTGGC 
GTGAGTGTTT GGAAAGGGTT GCAGGATATT CAGACGGTTG GTGATCTTGC ACTAAGAAAA 
AGCTCAGAGG AGGAGGCTGA CTCTATAGAT GCTGCCCAGA AGGCCTTCCG TCCAGAGCTG 
CAGGAGGAGC GGGCTCTTGC CGAGGCCCTG GTGTACCAGC GGCGGGAGCA GGTGGCACTC 
ATGGCTCTCC TGAATGGGAA GGCAGACGCG GAGGCTGAGG CTTGGGGTAA GCTGCAGAGG 
ATAACCCAGA TGTCCCCATG GGCTGGAGAG CACTACTCAG AAGACGTTGA GCACAGCTGG 
CTCGAGTGGG AGTCCAAGGG CAAGCAGGTG AGCAAAATCG TGGGAGGAGC ATACAAGGCC 
AAGGACCGGT CTCGGAGTCC CGGAGTCCTG TCCACTAATG TGCAGTATGG CTGGATGAAG 
CACCAGATTT CACCTCAGAT CTACCAAAGC CTGCACTTCA ACCAGCTGTC ACCCACCCAC 
ACACCTGACT TCCTGGTGCA CAATATCCTA GGGCATCACG GCCAGCACAT CTACCTGGTG 
ACCCACAATA GCCAAAAGGA CCTGGATCCC GAAGAGACCA ACTCGTCTAC TGCCTCTGTC 
TCCCCCAGCG ACACCCTCAC CCCGAAAGCC TCAGGCTCCG GGTTCCTGGA GTCCGCCTTG 
TCCACCTCCC CGTACCTCCA GCCCTCCCTC TCCCTCCTGT CCCAGCCATC CAGGTATGTC 
ACGCAGAGGT CGGCGCCCCC CATGCCTGTA GCCTCATCTA TCAAACTGCT CTCAAGACCT 
CTGAAGGCCG CCCTTTCATC CTCCATGAAG AAGAGCCACG GCGTCAGGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962694.1
CDS	1140..5033
Translation	MSQLRWKSKTNIFLSKIASKVTWEEQLGSGSKPSSSEDLRESPQSQEASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGALDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107219.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107219.2

ORF Insert Sequence:

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGGTGGGA GCGGAGGTGA GGCAGAAACA TGTCAGTTCA 
CAAAGCCCCA AGCTGTTAAG AATGCCCCAT GGCAAACAGT CCATCATCAG AGACGCTCAA 
AGGAAAAGAG GGGAATTCCA GAAGAAGATA CTACTGCAAT CCAACACATC GGTGGAGAAG 
ATAATCTTCC TGCAGACCAG GCCTCGCCTG CCACACATAG CTGCCCTGCT GAGCAGCTGC 
ATCGATGGCT ACATCTATGC CTGGTCCATC CACGGGAGCG GAGGCCTGTT GGGGAAGTTC 
CCTGTGGACT TCAAAAACCA GGGGGATGTG GGTGTGGGTG CCATGGCCAC GGATGAGAAA 
GACTTGATCC TTGTCACAGG AGATTGTAAA GGGCACATCA AGATTTGGGA CATCGAGGAT 
TATTGCATGT TCACCGACAA GACACCACTT CAACCCAGAG GGACAAACAA GTTCCAATTC 
TTAATTCCTG AGCAGGTCCA GATCAACCTG CCACACTACA TCCCCTTGAA GGAGAAGGAG 
GTCATGGCTG GCCAGACCAT TTCCCTGGTT CCCCCCAAGC TTCTGAATGC CTGGAAGGGC 
CATTTGGATA GTGTGGCAGA CATCCTGTAT GTGGATAGCT TCCAGCTGAT TATCAGTGCT 
GGCCACGATT GTGACGTCAA GGCTTGGAAA CTCTCCGGTG ACACCATTGG GACGTTTGGC 
GTGAGTGTTT GGAAAGGGTT GCAGGATATT CAGACGGTTG GTGATCTTGC ACTAAGAAAA 
AGCTCAGAGG AGGAGGCTGA CTCTATAGAT GCTGCCCAGA AGGCCTTCCG TCCAGAGCTG 
CAGGAGGAGC GGGCTCTTGC CGAGGCCCTG GTGTACCAGC GGCGGGAGCA GGTGGCACTC 
ATGGCTCTCC TGAATGGGAA GGCAGACGCG GAGGCTGAGG CTTGGGGTAA GCTGCAGAGG 
ATAACCCAGA TGTCCCCATG GGCTGGAGAG CACTACTCAG AAGACGTTGA GCACAGCTGG 
CTCGAGTGGG AGTCCAAGGG CAAGCAGGTG AGCAAAATCG TGGGAGGAGC ATACAAGGCC 
AAGGACCGGT CTCGGAGTCC CGGAGTCCTG TCCACTAATG TGCAGTATGG CTGGATGAAG 
CACCAGATTT CACCTCAGAT CTACCAAAGC CTGCACTTCA ACCAGCTGTC ACCCACCCAC 
ACACCTGACT TCCTGGTGCA CAATATCCTA GGGCATCACG GCCAGCACAT CTACCTGGTG 
ACCCACAATA GCCAAAAGGA CCTGGATCCC GAAGAGACCA ACTCGTCTAC TGCCTCTGTC 
TCCCCCAGCG ACACCCTCAC CCCGAAAGCC TCAGGCTCCG GGTTCCTGGA GTCCGCCTTG 
TCCACCTCCC CGTACCTCCA GCCCTCCCTC TCCCTCCTGT CCCAGCCATC CAGGTATGTC 
ACGCAGAGGT CGGCGCCCCC CATGCCTGTA GCCTCATCTA TCAAACTGCT CTCAAGACCT 
CTGAAGGCCG CCCTTTCATC CTCCATGAAG AAGAGCCACG GCGTCAGGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107220.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962695.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X2, mRNA.

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CloneID	OCe42869
Clone ID Related Accession (Same CDS sequence)	XM_014107220.1 , XM_014107220.2
Accession Version	XM_014107220.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGGTGGGA GCGGAGAAAC ATGTCAGTTC ACAAAGCCCC 
AAGCTGTTAA GAATGCCCCA TGGCAAACAG TCCATCATCA GAGACGCTCA AAGGAAAAGA 
GGGGAATTCC AGAAGAAGAT ACTACTGCAA TCCAACACAT CGGTGGAGAA GATAATCTTC 
CTGCAGACCA GGCCTCGCCT GCCACACATA GCTGCCCTGC TGAGCAGCTG CATCGATGGC 
TACATCTATG CCTGGTCCAT CCACGGGAGC GGAGGCCTGT TGGGGAAGTT CCCTGTGGAC 
TTCAAAAACC AGGGGGATGT GGGTGTGGGT GCCATGGCCA CGGATGAGAA AGACTTGATC 
CTTGTCACAG GAGATTGTAA AGGGCACATC AAGATTTGGG ACATCGAGGA TTATTGCATG 
TTCACCGACA AGACACCACT TCAACCCAGA GGGACAAACA AGTTCCAATT CTTAATTCCT 
GAGCAGGTCC AGATCAACCT GCCACACTAC ATCCCCTTGA AGGAGAAGGA GGTCATGGCT 
GGCCAGACCA TTTCCCTGGT TCCCCCCAAG CTTCTGAATG CCTGGAAGGG CCATTTGGAT 
AGTGTGGCAG ACATCCTGTA TGTGGATAGC TTCCAGCTGA TTATCAGTGC TGGCCACGAT 
TGTGACGTCA AGGCTTGGAA ACTCTCCGGT GACACCATTG GGACGTTTGG CGTGAGTGTT 
TGGAAAGGGT TGCAGGATAT TCAGACGGTT GGTGATCTTG CACTAAGAAA AAGCTCAGAG 
GAGGAGGCTG ACTCTATAGA TGCTGCCCAG AAGGCCTTCC GTCCAGAGCT GCAGGAGGAG 
CGGGCTCTTG CCGAGGCCCT GGTGTACCAG CGGCGGGAGC AGGTGGCACT CATGGCTCTC 
CTGAATGGGA AGGCAGACGC GGAGGCTGAG GCTTGGGGTA AGCTGCAGAG GATAACCCAG 
ATGTCCCCAT GGGCTGGAGA GCACTACTCA GAAGACGTTG AGCACAGCTG GCTCGAGTGG 
GAGTCCAAGG GCAAGCAGGT GAGCAAAATC GTGGGAGGAG CATACAAGGC CAAGGACCGG 
TCTCGGAGTC CCGGAGTCCT GTCCACTAAT GTGCAGTATG GCTGGATGAA GCACCAGATT 
TCACCTCAGA TCTACCAAAG CCTGCACTTC AACCAGCTGT CACCCACCCA CACACCTGAC 
TTCCTGGTGC ACAATATCCT AGGGCATCAC GGCCAGCACA TCTACCTGGT GACCCACAAT 
AGCCAAAAGG ACCTGGATCC CGAAGAGACC AACTCGTCTA CTGCCTCTGT CTCCCCCAGC 
GACACCCTCA CCCCGAAAGC CTCAGGCTCC GGGTTCCTGG AGTCCGCCTT GTCCACCTCC 
CCGTACCTCC AGCCCTCCCT CTCCCTCCTG TCCCAGCCAT CCAGGTATGT CACGCAGAGG 
TCGGCGCCCC CCATGCCTGT AGCCTCATCT ATCAAACTGC TCTCAAGACC TCTGAAGGCC 
GCCCTTTCAT CCTCCATGAA GAAGAGCCAC GGCGTCAGGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962695.1
CDS	1391..5275
Translation	MSQLRWKSKTNIFLSKIASKVTWEEQLGSGSKPSSSEDLRESPQSQEASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGALDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107220.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107220.1

ORF Insert Sequence:

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGGTGGGA GCGGAGAAAC ATGTCAGTTC ACAAAGCCCC 
AAGCTGTTAA GAATGCCCCA TGGCAAACAG TCCATCATCA GAGACGCTCA AAGGAAAAGA 
GGGGAATTCC AGAAGAAGAT ACTACTGCAA TCCAACACAT CGGTGGAGAA GATAATCTTC 
CTGCAGACCA GGCCTCGCCT GCCACACATA GCTGCCCTGC TGAGCAGCTG CATCGATGGC 
TACATCTATG CCTGGTCCAT CCACGGGAGC GGAGGCCTGT TGGGGAAGTT CCCTGTGGAC 
TTCAAAAACC AGGGGGATGT GGGTGTGGGT GCCATGGCCA CGGATGAGAA AGACTTGATC 
CTTGTCACAG GAGATTGTAA AGGGCACATC AAGATTTGGG ACATCGAGGA TTATTGCATG 
TTCACCGACA AGACACCACT TCAACCCAGA GGGACAAACA AGTTCCAATT CTTAATTCCT 
GAGCAGGTCC AGATCAACCT GCCACACTAC ATCCCCTTGA AGGAGAAGGA GGTCATGGCT 
GGCCAGACCA TTTCCCTGGT TCCCCCCAAG CTTCTGAATG CCTGGAAGGG CCATTTGGAT 
AGTGTGGCAG ACATCCTGTA TGTGGATAGC TTCCAGCTGA TTATCAGTGC TGGCCACGAT 
TGTGACGTCA AGGCTTGGAA ACTCTCCGGT GACACCATTG GGACGTTTGG CGTGAGTGTT 
TGGAAAGGGT TGCAGGATAT TCAGACGGTT GGTGATCTTG CACTAAGAAA AAGCTCAGAG 
GAGGAGGCTG ACTCTATAGA TGCTGCCCAG AAGGCCTTCC GTCCAGAGCT GCAGGAGGAG 
CGGGCTCTTG CCGAGGCCCT GGTGTACCAG CGGCGGGAGC AGGTGGCACT CATGGCTCTC 
CTGAATGGGA AGGCAGACGC GGAGGCTGAG GCTTGGGGTA AGCTGCAGAG GATAACCCAG 
ATGTCCCCAT GGGCTGGAGA GCACTACTCA GAAGACGTTG AGCACAGCTG GCTCGAGTGG 
GAGTCCAAGG GCAAGCAGGT GAGCAAAATC GTGGGAGGAG CATACAAGGC CAAGGACCGG 
TCTCGGAGTC CCGGAGTCCT GTCCACTAAT GTGCAGTATG GCTGGATGAA GCACCAGATT 
TCACCTCAGA TCTACCAAAG CCTGCACTTC AACCAGCTGT CACCCACCCA CACACCTGAC 
TTCCTGGTGC ACAATATCCT AGGGCATCAC GGCCAGCACA TCTACCTGGT GACCCACAAT 
AGCCAAAAGG ACCTGGATCC CGAAGAGACC AACTCGTCTA CTGCCTCTGT CTCCCCCAGC 
GACACCCTCA CCCCGAAAGC CTCAGGCTCC GGGTTCCTGG AGTCCGCCTT GTCCACCTCC 
CCGTACCTCC AGCCCTCCCT CTCCCTCCTG TCCCAGCCAT CCAGGTATGT CACGCAGAGG 
TCGGCGCCCC CCATGCCTGT AGCCTCATCT ATCAAACTGC TCTCAAGACC TCTGAAGGCC 
GCCCTTTCAT CCTCCATGAA GAAGAGCCAC GGCGTCAGGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107220.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962695.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X2, mRNA.

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CloneID	OCe42869
Clone ID Related Accession (Same CDS sequence)	XM_014107220.1 , XM_014107220.2
Accession Version	XM_014107220.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107220.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGGTGGGA GCGGAGAAAC ATGTCAGTTC ACAAAGCCCC 
AAGCTGTTAA GAATGCCCCA TGGCAAACAG TCCATCATCA GAGACGCTCA AAGGAAAAGA 
GGGGAATTCC AGAAGAAGAT ACTACTGCAA TCCAACACAT CGGTGGAGAA GATAATCTTC 
CTGCAGACCA GGCCTCGCCT GCCACACATA GCTGCCCTGC TGAGCAGCTG CATCGATGGC 
TACATCTATG CCTGGTCCAT CCACGGGAGC GGAGGCCTGT TGGGGAAGTT CCCTGTGGAC 
TTCAAAAACC AGGGGGATGT GGGTGTGGGT GCCATGGCCA CGGATGAGAA AGACTTGATC 
CTTGTCACAG GAGATTGTAA AGGGCACATC AAGATTTGGG ACATCGAGGA TTATTGCATG 
TTCACCGACA AGACACCACT TCAACCCAGA GGGACAAACA AGTTCCAATT CTTAATTCCT 
GAGCAGGTCC AGATCAACCT GCCACACTAC ATCCCCTTGA AGGAGAAGGA GGTCATGGCT 
GGCCAGACCA TTTCCCTGGT TCCCCCCAAG CTTCTGAATG CCTGGAAGGG CCATTTGGAT 
AGTGTGGCAG ACATCCTGTA TGTGGATAGC TTCCAGCTGA TTATCAGTGC TGGCCACGAT 
TGTGACGTCA AGGCTTGGAA ACTCTCCGGT GACACCATTG GGACGTTTGG CGTGAGTGTT 
TGGAAAGGGT TGCAGGATAT TCAGACGGTT GGTGATCTTG CACTAAGAAA AAGCTCAGAG 
GAGGAGGCTG ACTCTATAGA TGCTGCCCAG AAGGCCTTCC GTCCAGAGCT GCAGGAGGAG 
CGGGCTCTTG CCGAGGCCCT GGTGTACCAG CGGCGGGAGC AGGTGGCACT CATGGCTCTC 
CTGAATGGGA AGGCAGACGC GGAGGCTGAG GCTTGGGGTA AGCTGCAGAG GATAACCCAG 
ATGTCCCCAT GGGCTGGAGA GCACTACTCA GAAGACGTTG AGCACAGCTG GCTCGAGTGG 
GAGTCCAAGG GCAAGCAGGT GAGCAAAATC GTGGGAGGAG CATACAAGGC CAAGGACCGG 
TCTCGGAGTC CCGGAGTCCT GTCCACTAAT GTGCAGTATG GCTGGATGAA GCACCAGATT 
TCACCTCAGA TCTACCAAAG CCTGCACTTC AACCAGCTGT CACCCACCCA CACACCTGAC 
TTCCTGGTGC ACAATATCCT AGGGCATCAC GGCCAGCACA TCTACCTGGT GACCCACAAT 
AGCCAAAAGG ACCTGGATCC CGAAGAGACC AACTCGTCTA CTGCCTCTGT CTCCCCCAGC 
GACACCCTCA CCCCGAAAGC CTCAGGCTCC GGGTTCCTGG AGTCCGCCTT GTCCACCTCC 
CCGTACCTCC AGCCCTCCCT CTCCCTCCTG TCCCAGCCAT CCAGGTATGT CACGCAGAGG 
TCGGCGCCCC CCATGCCTGT AGCCTCATCT ATCAAACTGC TCTCAAGACC TCTGAAGGCC 
GCCCTTTCAT CCTCCATGAA GAAGAGCCAC GGCGTCAGGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962695.1
CDS	1140..5024
Translation	MSQLRWKSKTNIFLSKIASKVTWEEQLGSGSKPSSSEDLRESPQSQEASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGALDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107220.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107220.2

ORF Insert Sequence:

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGGTGGGA GCGGAGAAAC ATGTCAGTTC ACAAAGCCCC 
AAGCTGTTAA GAATGCCCCA TGGCAAACAG TCCATCATCA GAGACGCTCA AAGGAAAAGA 
GGGGAATTCC AGAAGAAGAT ACTACTGCAA TCCAACACAT CGGTGGAGAA GATAATCTTC 
CTGCAGACCA GGCCTCGCCT GCCACACATA GCTGCCCTGC TGAGCAGCTG CATCGATGGC 
TACATCTATG CCTGGTCCAT CCACGGGAGC GGAGGCCTGT TGGGGAAGTT CCCTGTGGAC 
TTCAAAAACC AGGGGGATGT GGGTGTGGGT GCCATGGCCA CGGATGAGAA AGACTTGATC 
CTTGTCACAG GAGATTGTAA AGGGCACATC AAGATTTGGG ACATCGAGGA TTATTGCATG 
TTCACCGACA AGACACCACT TCAACCCAGA GGGACAAACA AGTTCCAATT CTTAATTCCT 
GAGCAGGTCC AGATCAACCT GCCACACTAC ATCCCCTTGA AGGAGAAGGA GGTCATGGCT 
GGCCAGACCA TTTCCCTGGT TCCCCCCAAG CTTCTGAATG CCTGGAAGGG CCATTTGGAT 
AGTGTGGCAG ACATCCTGTA TGTGGATAGC TTCCAGCTGA TTATCAGTGC TGGCCACGAT 
TGTGACGTCA AGGCTTGGAA ACTCTCCGGT GACACCATTG GGACGTTTGG CGTGAGTGTT 
TGGAAAGGGT TGCAGGATAT TCAGACGGTT GGTGATCTTG CACTAAGAAA AAGCTCAGAG 
GAGGAGGCTG ACTCTATAGA TGCTGCCCAG AAGGCCTTCC GTCCAGAGCT GCAGGAGGAG 
CGGGCTCTTG CCGAGGCCCT GGTGTACCAG CGGCGGGAGC AGGTGGCACT CATGGCTCTC 
CTGAATGGGA AGGCAGACGC GGAGGCTGAG GCTTGGGGTA AGCTGCAGAG GATAACCCAG 
ATGTCCCCAT GGGCTGGAGA GCACTACTCA GAAGACGTTG AGCACAGCTG GCTCGAGTGG 
GAGTCCAAGG GCAAGCAGGT GAGCAAAATC GTGGGAGGAG CATACAAGGC CAAGGACCGG 
TCTCGGAGTC CCGGAGTCCT GTCCACTAAT GTGCAGTATG GCTGGATGAA GCACCAGATT 
TCACCTCAGA TCTACCAAAG CCTGCACTTC AACCAGCTGT CACCCACCCA CACACCTGAC 
TTCCTGGTGC ACAATATCCT AGGGCATCAC GGCCAGCACA TCTACCTGGT GACCCACAAT 
AGCCAAAAGG ACCTGGATCC CGAAGAGACC AACTCGTCTA CTGCCTCTGT CTCCCCCAGC 
GACACCCTCA CCCCGAAAGC CTCAGGCTCC GGGTTCCTGG AGTCCGCCTT GTCCACCTCC 
CCGTACCTCC AGCCCTCCCT CTCCCTCCTG TCCCAGCCAT CCAGGTATGT CACGCAGAGG 
TCGGCGCCCC CCATGCCTGT AGCCTCATCT ATCAAACTGC TCTCAAGACC TCTGAAGGCC 
GCCCTTTCAT CCTCCATGAA GAAGAGCCAC GGCGTCAGGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107221.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962696.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe42870
Clone ID Related Accession (Same CDS sequence)	XM_014107221.2 , XM_014107221.1
Accession Version	XM_014107221.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107221.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGAAACAT GTCAGTTCAC AAAGCCCCAA GCTGTTAAGA 
ATGCCCCATG GCAAACAGTC CATCATCAGA GACGCTCAAA GGAAAAGAGG GGAATTCCAG 
AAGAAGATAC TACTGCAATC CAACACATCG GTGGAGAAGA TAATCTTCCT GCAGACCAGG 
CCTCGCCTGC CACACATAGC TGCCCTGCTG AGCAGCTGCA TCGATGGCTA CATCTATGCC 
TGGTCCATCC ACGGGAGCGG AGGCCTGTTG GGGAAGTTCC CTGTGGACTT CAAAAACCAG 
GGGGATGTGG GTGTGGGTGC CATGGCCACG GATGAGAAAG ACTTGATCCT TGTCACAGGA 
GATTGTAAAG GGCACATCAA GATTTGGGAC ATCGAGGATT ATTGCATGTT CACCGACAAG 
ACACCACTTC AACCCAGAGG GACAAACAAG TTCCAATTCT TAATTCCTGA GCAGGTCCAG 
ATCAACCTGC CACACTACAT CCCCTTGAAG GAGAAGGAGG TCATGGCTGG CCAGACCATT 
TCCCTGGTTC CCCCCAAGCT TCTGAATGCC TGGAAGGGCC ATTTGGATAG TGTGGCAGAC 
ATCCTGTATG TGGATAGCTT CCAGCTGATT ATCAGTGCTG GCCACGATTG TGACGTCAAG 
GCTTGGAAAC TCTCCGGTGA CACCATTGGG ACGTTTGGCG TGAGTGTTTG GAAAGGGTTG 
CAGGATATTC AGACGGTTGG TGATCTTGCA CTAAGAAAAA GCTCAGAGGA GGAGGCTGAC 
TCTATAGATG CTGCCCAGAA GGCCTTCCGT CCAGAGCTGC AGGAGGAGCG GGCTCTTGCC 
GAGGCCCTGG TGTACCAGCG GCGGGAGCAG GTGGCACTCA TGGCTCTCCT GAATGGGAAG 
GCAGACGCGG AGGCTGAGGC TTGGGGTAAG CTGCAGAGGA TAACCCAGAT GTCCCCATGG 
GCTGGAGAGC ACTACTCAGA AGACGTTGAG CACAGCTGGC TCGAGTGGGA GTCCAAGGGC 
AAGCAGGTGA GCAAAATCGT GGGAGGAGCA TACAAGGCCA AGGACCGGTC TCGGAGTCCC 
GGAGTCCTGT CCACTAATGT GCAGTATGGC TGGATGAAGC ACCAGATTTC ACCTCAGATC 
TACCAAAGCC TGCACTTCAA CCAGCTGTCA CCCACCCACA CACCTGACTT CCTGGTGCAC 
AATATCCTAG GGCATCACGG CCAGCACATC TACCTGGTGA CCCACAATAG CCAAAAGGAC 
CTGGATCCCG AAGAGACCAA CTCGTCTACT GCCTCTGTCT CCCCCAGCGA CACCCTCACC 
CCGAAAGCCT CAGGCTCCGG GTTCCTGGAG TCCGCCTTGT CCACCTCCCC GTACCTCCAG 
CCCTCCCTCT CCCTCCTGTC CCAGCCATCC AGGTATGTCA CGCAGAGGTC GGCGCCCCCC 
ATGCCTGTAG CCTCATCTAT CAAACTGCTC TCAAGACCTC TGAAGGCCGC CCTTTCATCC 
TCCATGAAGA AGAGCCACGG CGTCAGGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962696.1
CDS	1140..5012
Translation	MSQLRWKSKTNIFLSKIASKVTWEEQLGSGSKPSSSEDLRESPQSQEASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGALDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107221.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107221.2

ORF Insert Sequence:

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGAAACAT GTCAGTTCAC AAAGCCCCAA GCTGTTAAGA 
ATGCCCCATG GCAAACAGTC CATCATCAGA GACGCTCAAA GGAAAAGAGG GGAATTCCAG 
AAGAAGATAC TACTGCAATC CAACACATCG GTGGAGAAGA TAATCTTCCT GCAGACCAGG 
CCTCGCCTGC CACACATAGC TGCCCTGCTG AGCAGCTGCA TCGATGGCTA CATCTATGCC 
TGGTCCATCC ACGGGAGCGG AGGCCTGTTG GGGAAGTTCC CTGTGGACTT CAAAAACCAG 
GGGGATGTGG GTGTGGGTGC CATGGCCACG GATGAGAAAG ACTTGATCCT TGTCACAGGA 
GATTGTAAAG GGCACATCAA GATTTGGGAC ATCGAGGATT ATTGCATGTT CACCGACAAG 
ACACCACTTC AACCCAGAGG GACAAACAAG TTCCAATTCT TAATTCCTGA GCAGGTCCAG 
ATCAACCTGC CACACTACAT CCCCTTGAAG GAGAAGGAGG TCATGGCTGG CCAGACCATT 
TCCCTGGTTC CCCCCAAGCT TCTGAATGCC TGGAAGGGCC ATTTGGATAG TGTGGCAGAC 
ATCCTGTATG TGGATAGCTT CCAGCTGATT ATCAGTGCTG GCCACGATTG TGACGTCAAG 
GCTTGGAAAC TCTCCGGTGA CACCATTGGG ACGTTTGGCG TGAGTGTTTG GAAAGGGTTG 
CAGGATATTC AGACGGTTGG TGATCTTGCA CTAAGAAAAA GCTCAGAGGA GGAGGCTGAC 
TCTATAGATG CTGCCCAGAA GGCCTTCCGT CCAGAGCTGC AGGAGGAGCG GGCTCTTGCC 
GAGGCCCTGG TGTACCAGCG GCGGGAGCAG GTGGCACTCA TGGCTCTCCT GAATGGGAAG 
GCAGACGCGG AGGCTGAGGC TTGGGGTAAG CTGCAGAGGA TAACCCAGAT GTCCCCATGG 
GCTGGAGAGC ACTACTCAGA AGACGTTGAG CACAGCTGGC TCGAGTGGGA GTCCAAGGGC 
AAGCAGGTGA GCAAAATCGT GGGAGGAGCA TACAAGGCCA AGGACCGGTC TCGGAGTCCC 
GGAGTCCTGT CCACTAATGT GCAGTATGGC TGGATGAAGC ACCAGATTTC ACCTCAGATC 
TACCAAAGCC TGCACTTCAA CCAGCTGTCA CCCACCCACA CACCTGACTT CCTGGTGCAC 
AATATCCTAG GGCATCACGG CCAGCACATC TACCTGGTGA CCCACAATAG CCAAAAGGAC 
CTGGATCCCG AAGAGACCAA CTCGTCTACT GCCTCTGTCT CCCCCAGCGA CACCCTCACC 
CCGAAAGCCT CAGGCTCCGG GTTCCTGGAG TCCGCCTTGT CCACCTCCCC GTACCTCCAG 
CCCTCCCTCT CCCTCCTGTC CCAGCCATCC AGGTATGTCA CGCAGAGGTC GGCGCCCCCC 
ATGCCTGTAG CCTCATCTAT CAAACTGCTC TCAAGACCTC TGAAGGCCGC CCTTTCATCC 
TCCATGAAGA AGAGCCACGG CGTCAGGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107221.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962696.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe42870
Clone ID Related Accession (Same CDS sequence)	XM_014107221.2 , XM_014107221.1
Accession Version	XM_014107221.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGAAACAT GTCAGTTCAC AAAGCCCCAA GCTGTTAAGA 
ATGCCCCATG GCAAACAGTC CATCATCAGA GACGCTCAAA GGAAAAGAGG GGAATTCCAG 
AAGAAGATAC TACTGCAATC CAACACATCG GTGGAGAAGA TAATCTTCCT GCAGACCAGG 
CCTCGCCTGC CACACATAGC TGCCCTGCTG AGCAGCTGCA TCGATGGCTA CATCTATGCC 
TGGTCCATCC ACGGGAGCGG AGGCCTGTTG GGGAAGTTCC CTGTGGACTT CAAAAACCAG 
GGGGATGTGG GTGTGGGTGC CATGGCCACG GATGAGAAAG ACTTGATCCT TGTCACAGGA 
GATTGTAAAG GGCACATCAA GATTTGGGAC ATCGAGGATT ATTGCATGTT CACCGACAAG 
ACACCACTTC AACCCAGAGG GACAAACAAG TTCCAATTCT TAATTCCTGA GCAGGTCCAG 
ATCAACCTGC CACACTACAT CCCCTTGAAG GAGAAGGAGG TCATGGCTGG CCAGACCATT 
TCCCTGGTTC CCCCCAAGCT TCTGAATGCC TGGAAGGGCC ATTTGGATAG TGTGGCAGAC 
ATCCTGTATG TGGATAGCTT CCAGCTGATT ATCAGTGCTG GCCACGATTG TGACGTCAAG 
GCTTGGAAAC TCTCCGGTGA CACCATTGGG ACGTTTGGCG TGAGTGTTTG GAAAGGGTTG 
CAGGATATTC AGACGGTTGG TGATCTTGCA CTAAGAAAAA GCTCAGAGGA GGAGGCTGAC 
TCTATAGATG CTGCCCAGAA GGCCTTCCGT CCAGAGCTGC AGGAGGAGCG GGCTCTTGCC 
GAGGCCCTGG TGTACCAGCG GCGGGAGCAG GTGGCACTCA TGGCTCTCCT GAATGGGAAG 
GCAGACGCGG AGGCTGAGGC TTGGGGTAAG CTGCAGAGGA TAACCCAGAT GTCCCCATGG 
GCTGGAGAGC ACTACTCAGA AGACGTTGAG CACAGCTGGC TCGAGTGGGA GTCCAAGGGC 
AAGCAGGTGA GCAAAATCGT GGGAGGAGCA TACAAGGCCA AGGACCGGTC TCGGAGTCCC 
GGAGTCCTGT CCACTAATGT GCAGTATGGC TGGATGAAGC ACCAGATTTC ACCTCAGATC 
TACCAAAGCC TGCACTTCAA CCAGCTGTCA CCCACCCACA CACCTGACTT CCTGGTGCAC 
AATATCCTAG GGCATCACGG CCAGCACATC TACCTGGTGA CCCACAATAG CCAAAAGGAC 
CTGGATCCCG AAGAGACCAA CTCGTCTACT GCCTCTGTCT CCCCCAGCGA CACCCTCACC 
CCGAAAGCCT CAGGCTCCGG GTTCCTGGAG TCCGCCTTGT CCACCTCCCC GTACCTCCAG 
CCCTCCCTCT CCCTCCTGTC CCAGCCATCC AGGTATGTCA CGCAGAGGTC GGCGCCCCCC 
ATGCCTGTAG CCTCATCTAT CAAACTGCTC TCAAGACCTC TGAAGGCCGC CCTTTCATCC 
TCCATGAAGA AGAGCCACGG CGTCAGGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962696.1
CDS	1391..5263
Translation	MSQLRWKSKTNIFLSKIASKVTWEEQLGSGSKPSSSEDLRESPQSQEASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGALDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107221.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107221.1

ORF Insert Sequence:

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGAAACAT GTCAGTTCAC AAAGCCCCAA GCTGTTAAGA 
ATGCCCCATG GCAAACAGTC CATCATCAGA GACGCTCAAA GGAAAAGAGG GGAATTCCAG 
AAGAAGATAC TACTGCAATC CAACACATCG GTGGAGAAGA TAATCTTCCT GCAGACCAGG 
CCTCGCCTGC CACACATAGC TGCCCTGCTG AGCAGCTGCA TCGATGGCTA CATCTATGCC 
TGGTCCATCC ACGGGAGCGG AGGCCTGTTG GGGAAGTTCC CTGTGGACTT CAAAAACCAG 
GGGGATGTGG GTGTGGGTGC CATGGCCACG GATGAGAAAG ACTTGATCCT TGTCACAGGA 
GATTGTAAAG GGCACATCAA GATTTGGGAC ATCGAGGATT ATTGCATGTT CACCGACAAG 
ACACCACTTC AACCCAGAGG GACAAACAAG TTCCAATTCT TAATTCCTGA GCAGGTCCAG 
ATCAACCTGC CACACTACAT CCCCTTGAAG GAGAAGGAGG TCATGGCTGG CCAGACCATT 
TCCCTGGTTC CCCCCAAGCT TCTGAATGCC TGGAAGGGCC ATTTGGATAG TGTGGCAGAC 
ATCCTGTATG TGGATAGCTT CCAGCTGATT ATCAGTGCTG GCCACGATTG TGACGTCAAG 
GCTTGGAAAC TCTCCGGTGA CACCATTGGG ACGTTTGGCG TGAGTGTTTG GAAAGGGTTG 
CAGGATATTC AGACGGTTGG TGATCTTGCA CTAAGAAAAA GCTCAGAGGA GGAGGCTGAC 
TCTATAGATG CTGCCCAGAA GGCCTTCCGT CCAGAGCTGC AGGAGGAGCG GGCTCTTGCC 
GAGGCCCTGG TGTACCAGCG GCGGGAGCAG GTGGCACTCA TGGCTCTCCT GAATGGGAAG 
GCAGACGCGG AGGCTGAGGC TTGGGGTAAG CTGCAGAGGA TAACCCAGAT GTCCCCATGG 
GCTGGAGAGC ACTACTCAGA AGACGTTGAG CACAGCTGGC TCGAGTGGGA GTCCAAGGGC 
AAGCAGGTGA GCAAAATCGT GGGAGGAGCA TACAAGGCCA AGGACCGGTC TCGGAGTCCC 
GGAGTCCTGT CCACTAATGT GCAGTATGGC TGGATGAAGC ACCAGATTTC ACCTCAGATC 
TACCAAAGCC TGCACTTCAA CCAGCTGTCA CCCACCCACA CACCTGACTT CCTGGTGCAC 
AATATCCTAG GGCATCACGG CCAGCACATC TACCTGGTGA CCCACAATAG CCAAAAGGAC 
CTGGATCCCG AAGAGACCAA CTCGTCTACT GCCTCTGTCT CCCCCAGCGA CACCCTCACC 
CCGAAAGCCT CAGGCTCCGG GTTCCTGGAG TCCGCCTTGT CCACCTCCCC GTACCTCCAG 
CCCTCCCTCT CCCTCCTGTC CCAGCCATCC AGGTATGTCA CGCAGAGGTC GGCGCCCCCC 
ATGCCTGTAG CCTCATCTAT CAAACTGCTC TCAAGACCTC TGAAGGCCGC CCTTTCATCC 
TCCATGAAGA AGAGCCACGG CGTCAGGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107222.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962697.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X4, mRNA.

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CloneID	OCe42871
Clone ID Related Accession (Same CDS sequence)	XM_014107222.2 , XM_014107222.1
Accession Version	XM_014107222.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107222.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGT TCTTAGCTCC CCAGCATCAA CTGTCACTCT TAGCCATCAT GAGTCTCAAC 
TGTTTACCCA GCTACACATG GTCAAGATGG AGAAAATGTT TGAGGATGAT GTTGACTCGA 
CTGGAGCTCC TACAGCTGCC CACTGTTCCT GGCCACATGG GACTCACCAA CATGGTGGAT 
TTCAAGGCTC AGAGTAGCCA TGAGATAGCA GCTCCAGCAA GATGTGTGCT ACAATCTTCC 
CTGGACATGA AGACTTTCAT TAAGGCCATA AAGAAGATTC TGAGCAATGC GTCAGATGAG 
ATGCTAGAGG CGCTGTTTTT GAAGGTGGAC ACAGACTGTA ATGGCTCCAT TACCTGGCAG 
GAGTACGTGG ATTACGTGAT GCGGGAGTTA CAGAGAAGGG AGAGGATGAG AACAAGCCAG 
TACCGCCTGC ACTTCCACCT TCCCATGAGG ATCATTCCCC TGAACCATGG ATGCGAGATT 
GTGAAGGTGC AGTTCTTAAC TCAGCGGTTC AAGAAGATGG GGCACCTTCT GACTGTCACC 
AAGGACGGGA TCTTGCAGAT CTGGTCTGAA TCCTTCTCGC TGCTTAACTC CTTTAGGCTT 
AACCAGATCC ATCAGCCCTA TAACCAGCAG ATGTGGGTCA CTGATATGGT GTGTCTCCAT 
AACATGAACC TGATTGCGAT TGCATCAACT GACCAGAAAA TAGAGTTCTT TGATATTACC 
AACCATAATT GTGCCCGGGC CTTCACCTTC ATTGATCTGG ACAGCTGTGT CCTGACCATG 
GACTATTGGT CCGACTATCA CAGAGGTGTA TTCTGCTACG GGGACACCAA AGGCAATGTC 
ATTGTCTTCA CCTCTGACAA TGTGACCCAC GGGCTGTTCA ACCCTCGAAT CCTTCCCAGG 
ACCTCCAAAT GGGATCACTG GACCAAAGTT TCTACACGGA AGCTTCTAAA TGAGAAGTCT 
CCTTTGTACA GGAGTTATTG GCTAAAGGCT CTCCATCCCA ACTGGTGCCA ACAGGTCAAG 
TTCATTCCTC AGCTGAACAC AGTGGCCTCC TGTTCGGCCA TTGAGAAGTC CTCTCTGGTG 
CTAATAGCAT TGCCATCTAA AGCCCCAGAG AGCCCCAAGT TTTCAGTGCT GAACTTAAGA 
AAAGGGATTC TTTGCTTCGA TTACTGCCCA GACAAGAACT TAGTGGTGAC TGGTGGCTAT 
GACCCCCTCA TCCGCCTGTG GAACCCCTTC TTCTCAAAAA AGCCTGTGTG GATGATGAAG 
GGGCATCAGA CTTCCGTGAC GCACATCCTC GTGAACAGCA AGAACAGCAG CATCCTCCTC 
AGCATCTCCA AGGACAAGAA TATCCGTGTG TGGGACATGC AGGACTACAT ATGCCTCCAG 
TCCTTTTCCG GGAAGCTTTT TGCCCTGGGA AACTGCCCCA TCACCAGTGT CTACTTCCAC 
AAGAATGAAG ACAGTCTCAT CTGTAGCACC TTTTCAATTG GGATCCTAAA AGGGTACTTG 
GAGACTGAGG GGCCTGGGAA ACCAAGGGAG AGGACCACAA CTTATAGCAC ACCCCTGTGC 
GCTGTTCTCT ACAGCAAGAT TTTTAAGCAG GTGGTAAGCG GCTGCCTGAG CAGCTTGGTG 
AGTGTGTGGG AGATCAAGAC AGGCAAGAGG ATGATGGAGT TCTCTGTGAC TGGCGACCAG 
CAAGTGGAGC TGACCACCAT GTGCCTGGAT GAATCCGAGC GGTGCCTGCT CACGGGTTTG 
CGCGACGGCA CCATAAAGAT GTGGAACTAC ACTGTGGGTG AATGCCTGCT GACCTTCCCC 
AACCCGGACC AGCTGGAGAT TAGCGGGATT GTCCATATGA ACAAAGTGTT CTACGTGACT 
GGATGGAGTA AGAGAATCAC CTGTTTCATG CTCCATAAGA CCAAGCCAGT CCTCCTGTGC 
TACCAATGGC AGAGCTTCCA CAAGGAAGAT GTTCTGAGCA TGGCCAAGTA CCAAAACCAG 
TTCCTTGGGA CCTCTTCCTA CAATGGAGAT ATTCTCTTCT GGAACATCAA CCTGTTCAAG 
CCCATCCTCA ATTTCAATGC TTCTAAGAGC CCCTGGCCTT TGCTGCCCAA GAAGATCCAA 
GAAGTGGACA ACTCCCTGGC TGAGAGCCTC CAGTCCAGCA AGCTTTGTGT GAAACATAAG 
GAGTGGGCGT ACAAACCATC AACGCAGCCC CTGGGCCCCA GAGCCAGGGC TGTAGCCAAT 
GCCAACCTGC AACGGAACCT GATGTCAGCT CCCCCAGTGA TGAAGCGCCC CAGAGACAAG 
GAGCCAGAAA AGCCCATGCC CCCGCAGGTG GGAGCGGAGG TGAGGCAGAA ACATGTCAGT 
TCACAAAGCC CCAAGCTGTT AAGAATGCCC CATGGCAAAC AGTCCATCAT CAGAGACGCT 
CAAAGGAAAA GAGGGGAATT CCAGAAGAAG ATACTACTGC AATCCAACAC ATCGGTGGAG 
AAGATAATCT TCCTGCAGAC CAGGCCTCGC CTGCCACACA TAGCTGCCCT GCTGAGCAGC 
TGCATCGATG GCTACATCTA TGCCTGGTCC ATCCACGGGA GCGGAGGCCT GTTGGGGAAG 
TTCCCTGTGG ACTTCAAAAA CCAGGGGGAT GTGGGTGTGG GTGCCATGGC CACGGATGAG 
AAAGACTTGA TCCTTGTCAC AGGAGATTGT AAAGGGCACA TCAAGATTTG GGACATCGAG 
GATTATTGCA TGTTCACCGA CAAGACACCA CTTCAACCCA GAGGGACAAA CAAGTTCCAA 
TTCTTAATTC CTGAGCAGGT CCAGATCAAC CTGCCACACT ACATCCCCTT GAAGGAGAAG 
GAGGTCATGG CTGGCCAGAC CATTTCCCTG GTTCCCCCCA AGCTTCTGAA TGCCTGGAAG 
GGCCATTTGG ATAGTGTGGC AGACATCCTG TATGTGGATA GCTTCCAGCT GATTATCAGT 
GCTGGCCACG ATTGTGACGT CAAGGCTTGG AAACTCTCCG GTGACACCAT TGGGACGTTT 
GGCGTGAGTG TTTGGAAAGG GTTGCAGGAT ATTCAGACGG TTGGTGATCT TGCACTAAGA 
AAAAGCTCAG AGGAGGAGGC TGACTCTATA GATGCTGCCC AGAAGGCCTT CCGTCCAGAG 
CTGCAGGAGG AGCGGGCTCT TGCCGAGGCC CTGGTGTACC AGCGGCGGGA GCAGGTGGCA 
CTCATGGCTC TCCTGAATGG GAAGGCAGAC GCGGAGGCTG AGGCTTGGGG TAAGCTGCAG 
AGGATAACCC AGATGTCCCC ATGGGCTGGA GAGCACTACT CAGAAGACGT TGAGCACAGC 
TGGCTCGAGT GGGAGTCCAA GGGCAAGCAG GTGAGCAAAA TCGTGGGAGG AGCATACAAG 
GCCAAGGACC GGTCTCGGAG TCCCGGAGTC CTGTCCACTA ATGTGCAGTA TGGCTGGATG 
AAGCACCAGA TTTCACCTCA GATCTACCAA AGCCTGCACT TCAACCAGCT GTCACCCACC 
CACACACCTG ACTTCCTGGT GCACAATATC CTAGGGCATC ACGGCCAGCA CATCTACCTG 
GTGACCCACA ATAGCCAAAA GGACCTGGAT CCCGAAGAGA CCAACTCGTC TACTGCCTCT 
GTCTCCCCCA GCGACACCCT CACCCCGAAA GCCTCAGGCT CCGGGTTCCT GGAGTCCGCC 
TTGTCCACCT CCCCGTACCT CCAGCCCTCC CTCTCCCTCC TGTCCCAGCC ATCCAGGTAT 
GTCACGCAGA GGTCGGCGCC CCCCATGCCT GTAGCCTCAT CTATCAAACT GCTCTCAAGA 
CCTCTGAAGG CCGCCCTTTC ATCCTCCATG AAGAAGAGCC ACGGCGTCAG GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962697.1
CDS	5..3841
Translation	MRRFLAPQHQLSLLAIMSLNCLPSYTWSRWRKCLRMMLTRLELLQLPTVPGHMGLTNMVDFKAQSSHEIAAPARCVLQSSLDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107222.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107222.2

ORF Insert Sequence:

ATGAGGAGGT TCTTAGCTCC CCAGCATCAA CTGTCACTCT TAGCCATCAT GAGTCTCAAC 
TGTTTACCCA GCTACACATG GTCAAGATGG AGAAAATGTT TGAGGATGAT GTTGACTCGA 
CTGGAGCTCC TACAGCTGCC CACTGTTCCT GGCCACATGG GACTCACCAA CATGGTGGAT 
TTCAAGGCTC AGAGTAGCCA TGAGATAGCA GCTCCAGCAA GATGTGTGCT ACAATCTTCC 
CTGGACATGA AGACTTTCAT TAAGGCCATA AAGAAGATTC TGAGCAATGC GTCAGATGAG 
ATGCTAGAGG CGCTGTTTTT GAAGGTGGAC ACAGACTGTA ATGGCTCCAT TACCTGGCAG 
GAGTACGTGG ATTACGTGAT GCGGGAGTTA CAGAGAAGGG AGAGGATGAG AACAAGCCAG 
TACCGCCTGC ACTTCCACCT TCCCATGAGG ATCATTCCCC TGAACCATGG ATGCGAGATT 
GTGAAGGTGC AGTTCTTAAC TCAGCGGTTC AAGAAGATGG GGCACCTTCT GACTGTCACC 
AAGGACGGGA TCTTGCAGAT CTGGTCTGAA TCCTTCTCGC TGCTTAACTC CTTTAGGCTT 
AACCAGATCC ATCAGCCCTA TAACCAGCAG ATGTGGGTCA CTGATATGGT GTGTCTCCAT 
AACATGAACC TGATTGCGAT TGCATCAACT GACCAGAAAA TAGAGTTCTT TGATATTACC 
AACCATAATT GTGCCCGGGC CTTCACCTTC ATTGATCTGG ACAGCTGTGT CCTGACCATG 
GACTATTGGT CCGACTATCA CAGAGGTGTA TTCTGCTACG GGGACACCAA AGGCAATGTC 
ATTGTCTTCA CCTCTGACAA TGTGACCCAC GGGCTGTTCA ACCCTCGAAT CCTTCCCAGG 
ACCTCCAAAT GGGATCACTG GACCAAAGTT TCTACACGGA AGCTTCTAAA TGAGAAGTCT 
CCTTTGTACA GGAGTTATTG GCTAAAGGCT CTCCATCCCA ACTGGTGCCA ACAGGTCAAG 
TTCATTCCTC AGCTGAACAC AGTGGCCTCC TGTTCGGCCA TTGAGAAGTC CTCTCTGGTG 
CTAATAGCAT TGCCATCTAA AGCCCCAGAG AGCCCCAAGT TTTCAGTGCT GAACTTAAGA 
AAAGGGATTC TTTGCTTCGA TTACTGCCCA GACAAGAACT TAGTGGTGAC TGGTGGCTAT 
GACCCCCTCA TCCGCCTGTG GAACCCCTTC TTCTCAAAAA AGCCTGTGTG GATGATGAAG 
GGGCATCAGA CTTCCGTGAC GCACATCCTC GTGAACAGCA AGAACAGCAG CATCCTCCTC 
AGCATCTCCA AGGACAAGAA TATCCGTGTG TGGGACATGC AGGACTACAT ATGCCTCCAG 
TCCTTTTCCG GGAAGCTTTT TGCCCTGGGA AACTGCCCCA TCACCAGTGT CTACTTCCAC 
AAGAATGAAG ACAGTCTCAT CTGTAGCACC TTTTCAATTG GGATCCTAAA AGGGTACTTG 
GAGACTGAGG GGCCTGGGAA ACCAAGGGAG AGGACCACAA CTTATAGCAC ACCCCTGTGC 
GCTGTTCTCT ACAGCAAGAT TTTTAAGCAG GTGGTAAGCG GCTGCCTGAG CAGCTTGGTG 
AGTGTGTGGG AGATCAAGAC AGGCAAGAGG ATGATGGAGT TCTCTGTGAC TGGCGACCAG 
CAAGTGGAGC TGACCACCAT GTGCCTGGAT GAATCCGAGC GGTGCCTGCT CACGGGTTTG 
CGCGACGGCA CCATAAAGAT GTGGAACTAC ACTGTGGGTG AATGCCTGCT GACCTTCCCC 
AACCCGGACC AGCTGGAGAT TAGCGGGATT GTCCATATGA ACAAAGTGTT CTACGTGACT 
GGATGGAGTA AGAGAATCAC CTGTTTCATG CTCCATAAGA CCAAGCCAGT CCTCCTGTGC 
TACCAATGGC AGAGCTTCCA CAAGGAAGAT GTTCTGAGCA TGGCCAAGTA CCAAAACCAG 
TTCCTTGGGA CCTCTTCCTA CAATGGAGAT ATTCTCTTCT GGAACATCAA CCTGTTCAAG 
CCCATCCTCA ATTTCAATGC TTCTAAGAGC CCCTGGCCTT TGCTGCCCAA GAAGATCCAA 
GAAGTGGACA ACTCCCTGGC TGAGAGCCTC CAGTCCAGCA AGCTTTGTGT GAAACATAAG 
GAGTGGGCGT ACAAACCATC AACGCAGCCC CTGGGCCCCA GAGCCAGGGC TGTAGCCAAT 
GCCAACCTGC AACGGAACCT GATGTCAGCT CCCCCAGTGA TGAAGCGCCC CAGAGACAAG 
GAGCCAGAAA AGCCCATGCC CCCGCAGGTG GGAGCGGAGG TGAGGCAGAA ACATGTCAGT 
TCACAAAGCC CCAAGCTGTT AAGAATGCCC CATGGCAAAC AGTCCATCAT CAGAGACGCT 
CAAAGGAAAA GAGGGGAATT CCAGAAGAAG ATACTACTGC AATCCAACAC ATCGGTGGAG 
AAGATAATCT TCCTGCAGAC CAGGCCTCGC CTGCCACACA TAGCTGCCCT GCTGAGCAGC 
TGCATCGATG GCTACATCTA TGCCTGGTCC ATCCACGGGA GCGGAGGCCT GTTGGGGAAG 
TTCCCTGTGG ACTTCAAAAA CCAGGGGGAT GTGGGTGTGG GTGCCATGGC CACGGATGAG 
AAAGACTTGA TCCTTGTCAC AGGAGATTGT AAAGGGCACA TCAAGATTTG GGACATCGAG 
GATTATTGCA TGTTCACCGA CAAGACACCA CTTCAACCCA GAGGGACAAA CAAGTTCCAA 
TTCTTAATTC CTGAGCAGGT CCAGATCAAC CTGCCACACT ACATCCCCTT GAAGGAGAAG 
GAGGTCATGG CTGGCCAGAC CATTTCCCTG GTTCCCCCCA AGCTTCTGAA TGCCTGGAAG 
GGCCATTTGG ATAGTGTGGC AGACATCCTG TATGTGGATA GCTTCCAGCT GATTATCAGT 
GCTGGCCACG ATTGTGACGT CAAGGCTTGG AAACTCTCCG GTGACACCAT TGGGACGTTT 
GGCGTGAGTG TTTGGAAAGG GTTGCAGGAT ATTCAGACGG TTGGTGATCT TGCACTAAGA 
AAAAGCTCAG AGGAGGAGGC TGACTCTATA GATGCTGCCC AGAAGGCCTT CCGTCCAGAG 
CTGCAGGAGG AGCGGGCTCT TGCCGAGGCC CTGGTGTACC AGCGGCGGGA GCAGGTGGCA 
CTCATGGCTC TCCTGAATGG GAAGGCAGAC GCGGAGGCTG AGGCTTGGGG TAAGCTGCAG 
AGGATAACCC AGATGTCCCC ATGGGCTGGA GAGCACTACT CAGAAGACGT TGAGCACAGC 
TGGCTCGAGT GGGAGTCCAA GGGCAAGCAG GTGAGCAAAA TCGTGGGAGG AGCATACAAG 
GCCAAGGACC GGTCTCGGAG TCCCGGAGTC CTGTCCACTA ATGTGCAGTA TGGCTGGATG 
AAGCACCAGA TTTCACCTCA GATCTACCAA AGCCTGCACT TCAACCAGCT GTCACCCACC 
CACACACCTG ACTTCCTGGT GCACAATATC CTAGGGCATC ACGGCCAGCA CATCTACCTG 
GTGACCCACA ATAGCCAAAA GGACCTGGAT CCCGAAGAGA CCAACTCGTC TACTGCCTCT 
GTCTCCCCCA GCGACACCCT CACCCCGAAA GCCTCAGGCT CCGGGTTCCT GGAGTCCGCC 
TTGTCCACCT CCCCGTACCT CCAGCCCTCC CTCTCCCTCC TGTCCCAGCC ATCCAGGTAT 
GTCACGCAGA GGTCGGCGCC CCCCATGCCT GTAGCCTCAT CTATCAAACT GCTCTCAAGA 
CCTCTGAAGG CCGCCCTTTC ATCCTCCATG AAGAAGAGCC ACGGCGTCAG GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107222.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962697.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe42871
Clone ID Related Accession (Same CDS sequence)	XM_014107222.2 , XM_014107222.1
Accession Version	XM_014107222.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGT TCTTAGCTCC CCAGCATCAA CTGTCACTCT TAGCCATCAT GAGTCTCAAC 
TGTTTACCCA GCTACACATG GTCAAGATGG AGAAAATGTT TGAGGATGAT GTTGACTCGA 
CTGGAGCTCC TACAGCTGCC CACTGTTCCT GGCCACATGG GACTCACCAA CATGGTGGAT 
TTCAAGGCTC AGAGTAGCCA TGAGATAGCA GCTCCAGCAA GATGTGTGCT ACAATCTTCC 
CTGGACATGA AGACTTTCAT TAAGGCCATA AAGAAGATTC TGAGCAATGC GTCAGATGAG 
ATGCTAGAGG CGCTGTTTTT GAAGGTGGAC ACAGACTGTA ATGGCTCCAT TACCTGGCAG 
GAGTACGTGG ATTACGTGAT GCGGGAGTTA CAGAGAAGGG AGAGGATGAG AACAAGCCAG 
TACCGCCTGC ACTTCCACCT TCCCATGAGG ATCATTCCCC TGAACCATGG ATGCGAGATT 
GTGAAGGTGC AGTTCTTAAC TCAGCGGTTC AAGAAGATGG GGCACCTTCT GACTGTCACC 
AAGGACGGGA TCTTGCAGAT CTGGTCTGAA TCCTTCTCGC TGCTTAACTC CTTTAGGCTT 
AACCAGATCC ATCAGCCCTA TAACCAGCAG ATGTGGGTCA CTGATATGGT GTGTCTCCAT 
AACATGAACC TGATTGCGAT TGCATCAACT GACCAGAAAA TAGAGTTCTT TGATATTACC 
AACCATAATT GTGCCCGGGC CTTCACCTTC ATTGATCTGG ACAGCTGTGT CCTGACCATG 
GACTATTGGT CCGACTATCA CAGAGGTGTA TTCTGCTACG GGGACACCAA AGGCAATGTC 
ATTGTCTTCA CCTCTGACAA TGTGACCCAC GGGCTGTTCA ACCCTCGAAT CCTTCCCAGG 
ACCTCCAAAT GGGATCACTG GACCAAAGTT TCTACACGGA AGCTTCTAAA TGAGAAGTCT 
CCTTTGTACA GGAGTTATTG GCTAAAGGCT CTCCATCCCA ACTGGTGCCA ACAGGTCAAG 
TTCATTCCTC AGCTGAACAC AGTGGCCTCC TGTTCGGCCA TTGAGAAGTC CTCTCTGGTG 
CTAATAGCAT TGCCATCTAA AGCCCCAGAG AGCCCCAAGT TTTCAGTGCT GAACTTAAGA 
AAAGGGATTC TTTGCTTCGA TTACTGCCCA GACAAGAACT TAGTGGTGAC TGGTGGCTAT 
GACCCCCTCA TCCGCCTGTG GAACCCCTTC TTCTCAAAAA AGCCTGTGTG GATGATGAAG 
GGGCATCAGA CTTCCGTGAC GCACATCCTC GTGAACAGCA AGAACAGCAG CATCCTCCTC 
AGCATCTCCA AGGACAAGAA TATCCGTGTG TGGGACATGC AGGACTACAT ATGCCTCCAG 
TCCTTTTCCG GGAAGCTTTT TGCCCTGGGA AACTGCCCCA TCACCAGTGT CTACTTCCAC 
AAGAATGAAG ACAGTCTCAT CTGTAGCACC TTTTCAATTG GGATCCTAAA AGGGTACTTG 
GAGACTGAGG GGCCTGGGAA ACCAAGGGAG AGGACCACAA CTTATAGCAC ACCCCTGTGC 
GCTGTTCTCT ACAGCAAGAT TTTTAAGCAG GTGGTAAGCG GCTGCCTGAG CAGCTTGGTG 
AGTGTGTGGG AGATCAAGAC AGGCAAGAGG ATGATGGAGT TCTCTGTGAC TGGCGACCAG 
CAAGTGGAGC TGACCACCAT GTGCCTGGAT GAATCCGAGC GGTGCCTGCT CACGGGTTTG 
CGCGACGGCA CCATAAAGAT GTGGAACTAC ACTGTGGGTG AATGCCTGCT GACCTTCCCC 
AACCCGGACC AGCTGGAGAT TAGCGGGATT GTCCATATGA ACAAAGTGTT CTACGTGACT 
GGATGGAGTA AGAGAATCAC CTGTTTCATG CTCCATAAGA CCAAGCCAGT CCTCCTGTGC 
TACCAATGGC AGAGCTTCCA CAAGGAAGAT GTTCTGAGCA TGGCCAAGTA CCAAAACCAG 
TTCCTTGGGA CCTCTTCCTA CAATGGAGAT ATTCTCTTCT GGAACATCAA CCTGTTCAAG 
CCCATCCTCA ATTTCAATGC TTCTAAGAGC CCCTGGCCTT TGCTGCCCAA GAAGATCCAA 
GAAGTGGACA ACTCCCTGGC TGAGAGCCTC CAGTCCAGCA AGCTTTGTGT GAAACATAAG 
GAGTGGGCGT ACAAACCATC AACGCAGCCC CTGGGCCCCA GAGCCAGGGC TGTAGCCAAT 
GCCAACCTGC AACGGAACCT GATGTCAGCT CCCCCAGTGA TGAAGCGCCC CAGAGACAAG 
GAGCCAGAAA AGCCCATGCC CCCGCAGGTG GGAGCGGAGG TGAGGCAGAA ACATGTCAGT 
TCACAAAGCC CCAAGCTGTT AAGAATGCCC CATGGCAAAC AGTCCATCAT CAGAGACGCT 
CAAAGGAAAA GAGGGGAATT CCAGAAGAAG ATACTACTGC AATCCAACAC ATCGGTGGAG 
AAGATAATCT TCCTGCAGAC CAGGCCTCGC CTGCCACACA TAGCTGCCCT GCTGAGCAGC 
TGCATCGATG GCTACATCTA TGCCTGGTCC ATCCACGGGA GCGGAGGCCT GTTGGGGAAG 
TTCCCTGTGG ACTTCAAAAA CCAGGGGGAT GTGGGTGTGG GTGCCATGGC CACGGATGAG 
AAAGACTTGA TCCTTGTCAC AGGAGATTGT AAAGGGCACA TCAAGATTTG GGACATCGAG 
GATTATTGCA TGTTCACCGA CAAGACACCA CTTCAACCCA GAGGGACAAA CAAGTTCCAA 
TTCTTAATTC CTGAGCAGGT CCAGATCAAC CTGCCACACT ACATCCCCTT GAAGGAGAAG 
GAGGTCATGG CTGGCCAGAC CATTTCCCTG GTTCCCCCCA AGCTTCTGAA TGCCTGGAAG 
GGCCATTTGG ATAGTGTGGC AGACATCCTG TATGTGGATA GCTTCCAGCT GATTATCAGT 
GCTGGCCACG ATTGTGACGT CAAGGCTTGG AAACTCTCCG GTGACACCAT TGGGACGTTT 
GGCGTGAGTG TTTGGAAAGG GTTGCAGGAT ATTCAGACGG TTGGTGATCT TGCACTAAGA 
AAAAGCTCAG AGGAGGAGGC TGACTCTATA GATGCTGCCC AGAAGGCCTT CCGTCCAGAG 
CTGCAGGAGG AGCGGGCTCT TGCCGAGGCC CTGGTGTACC AGCGGCGGGA GCAGGTGGCA 
CTCATGGCTC TCCTGAATGG GAAGGCAGAC GCGGAGGCTG AGGCTTGGGG TAAGCTGCAG 
AGGATAACCC AGATGTCCCC ATGGGCTGGA GAGCACTACT CAGAAGACGT TGAGCACAGC 
TGGCTCGAGT GGGAGTCCAA GGGCAAGCAG GTGAGCAAAA TCGTGGGAGG AGCATACAAG 
GCCAAGGACC GGTCTCGGAG TCCCGGAGTC CTGTCCACTA ATGTGCAGTA TGGCTGGATG 
AAGCACCAGA TTTCACCTCA GATCTACCAA AGCCTGCACT TCAACCAGCT GTCACCCACC 
CACACACCTG ACTTCCTGGT GCACAATATC CTAGGGCATC ACGGCCAGCA CATCTACCTG 
GTGACCCACA ATAGCCAAAA GGACCTGGAT CCCGAAGAGA CCAACTCGTC TACTGCCTCT 
GTCTCCCCCA GCGACACCCT CACCCCGAAA GCCTCAGGCT CCGGGTTCCT GGAGTCCGCC 
TTGTCCACCT CCCCGTACCT CCAGCCCTCC CTCTCCCTCC TGTCCCAGCC ATCCAGGTAT 
GTCACGCAGA GGTCGGCGCC CCCCATGCCT GTAGCCTCAT CTATCAAACT GCTCTCAAGA 
CCTCTGAAGG CCGCCCTTTC ATCCTCCATG AAGAAGAGCC ACGGCGTCAG GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962697.1
CDS	2348..6184
Translation	MRRFLAPQHQLSLLAIMSLNCLPSYTWSRWRKCLRMMLTRLELLQLPTVPGHMGLTNMVDFKAQSSHEIAAPARCVLQSSLDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107222.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107222.1

ORF Insert Sequence:

ATGAGGAGGT TCTTAGCTCC CCAGCATCAA CTGTCACTCT TAGCCATCAT GAGTCTCAAC 
TGTTTACCCA GCTACACATG GTCAAGATGG AGAAAATGTT TGAGGATGAT GTTGACTCGA 
CTGGAGCTCC TACAGCTGCC CACTGTTCCT GGCCACATGG GACTCACCAA CATGGTGGAT 
TTCAAGGCTC AGAGTAGCCA TGAGATAGCA GCTCCAGCAA GATGTGTGCT ACAATCTTCC 
CTGGACATGA AGACTTTCAT TAAGGCCATA AAGAAGATTC TGAGCAATGC GTCAGATGAG 
ATGCTAGAGG CGCTGTTTTT GAAGGTGGAC ACAGACTGTA ATGGCTCCAT TACCTGGCAG 
GAGTACGTGG ATTACGTGAT GCGGGAGTTA CAGAGAAGGG AGAGGATGAG AACAAGCCAG 
TACCGCCTGC ACTTCCACCT TCCCATGAGG ATCATTCCCC TGAACCATGG ATGCGAGATT 
GTGAAGGTGC AGTTCTTAAC TCAGCGGTTC AAGAAGATGG GGCACCTTCT GACTGTCACC 
AAGGACGGGA TCTTGCAGAT CTGGTCTGAA TCCTTCTCGC TGCTTAACTC CTTTAGGCTT 
AACCAGATCC ATCAGCCCTA TAACCAGCAG ATGTGGGTCA CTGATATGGT GTGTCTCCAT 
AACATGAACC TGATTGCGAT TGCATCAACT GACCAGAAAA TAGAGTTCTT TGATATTACC 
AACCATAATT GTGCCCGGGC CTTCACCTTC ATTGATCTGG ACAGCTGTGT CCTGACCATG 
GACTATTGGT CCGACTATCA CAGAGGTGTA TTCTGCTACG GGGACACCAA AGGCAATGTC 
ATTGTCTTCA CCTCTGACAA TGTGACCCAC GGGCTGTTCA ACCCTCGAAT CCTTCCCAGG 
ACCTCCAAAT GGGATCACTG GACCAAAGTT TCTACACGGA AGCTTCTAAA TGAGAAGTCT 
CCTTTGTACA GGAGTTATTG GCTAAAGGCT CTCCATCCCA ACTGGTGCCA ACAGGTCAAG 
TTCATTCCTC AGCTGAACAC AGTGGCCTCC TGTTCGGCCA TTGAGAAGTC CTCTCTGGTG 
CTAATAGCAT TGCCATCTAA AGCCCCAGAG AGCCCCAAGT TTTCAGTGCT GAACTTAAGA 
AAAGGGATTC TTTGCTTCGA TTACTGCCCA GACAAGAACT TAGTGGTGAC TGGTGGCTAT 
GACCCCCTCA TCCGCCTGTG GAACCCCTTC TTCTCAAAAA AGCCTGTGTG GATGATGAAG 
GGGCATCAGA CTTCCGTGAC GCACATCCTC GTGAACAGCA AGAACAGCAG CATCCTCCTC 
AGCATCTCCA AGGACAAGAA TATCCGTGTG TGGGACATGC AGGACTACAT ATGCCTCCAG 
TCCTTTTCCG GGAAGCTTTT TGCCCTGGGA AACTGCCCCA TCACCAGTGT CTACTTCCAC 
AAGAATGAAG ACAGTCTCAT CTGTAGCACC TTTTCAATTG GGATCCTAAA AGGGTACTTG 
GAGACTGAGG GGCCTGGGAA ACCAAGGGAG AGGACCACAA CTTATAGCAC ACCCCTGTGC 
GCTGTTCTCT ACAGCAAGAT TTTTAAGCAG GTGGTAAGCG GCTGCCTGAG CAGCTTGGTG 
AGTGTGTGGG AGATCAAGAC AGGCAAGAGG ATGATGGAGT TCTCTGTGAC TGGCGACCAG 
CAAGTGGAGC TGACCACCAT GTGCCTGGAT GAATCCGAGC GGTGCCTGCT CACGGGTTTG 
CGCGACGGCA CCATAAAGAT GTGGAACTAC ACTGTGGGTG AATGCCTGCT GACCTTCCCC 
AACCCGGACC AGCTGGAGAT TAGCGGGATT GTCCATATGA ACAAAGTGTT CTACGTGACT 
GGATGGAGTA AGAGAATCAC CTGTTTCATG CTCCATAAGA CCAAGCCAGT CCTCCTGTGC 
TACCAATGGC AGAGCTTCCA CAAGGAAGAT GTTCTGAGCA TGGCCAAGTA CCAAAACCAG 
TTCCTTGGGA CCTCTTCCTA CAATGGAGAT ATTCTCTTCT GGAACATCAA CCTGTTCAAG 
CCCATCCTCA ATTTCAATGC TTCTAAGAGC CCCTGGCCTT TGCTGCCCAA GAAGATCCAA 
GAAGTGGACA ACTCCCTGGC TGAGAGCCTC CAGTCCAGCA AGCTTTGTGT GAAACATAAG 
GAGTGGGCGT ACAAACCATC AACGCAGCCC CTGGGCCCCA GAGCCAGGGC TGTAGCCAAT 
GCCAACCTGC AACGGAACCT GATGTCAGCT CCCCCAGTGA TGAAGCGCCC CAGAGACAAG 
GAGCCAGAAA AGCCCATGCC CCCGCAGGTG GGAGCGGAGG TGAGGCAGAA ACATGTCAGT 
TCACAAAGCC CCAAGCTGTT AAGAATGCCC CATGGCAAAC AGTCCATCAT CAGAGACGCT 
CAAAGGAAAA GAGGGGAATT CCAGAAGAAG ATACTACTGC AATCCAACAC ATCGGTGGAG 
AAGATAATCT TCCTGCAGAC CAGGCCTCGC CTGCCACACA TAGCTGCCCT GCTGAGCAGC 
TGCATCGATG GCTACATCTA TGCCTGGTCC ATCCACGGGA GCGGAGGCCT GTTGGGGAAG 
TTCCCTGTGG ACTTCAAAAA CCAGGGGGAT GTGGGTGTGG GTGCCATGGC CACGGATGAG 
AAAGACTTGA TCCTTGTCAC AGGAGATTGT AAAGGGCACA TCAAGATTTG GGACATCGAG 
GATTATTGCA TGTTCACCGA CAAGACACCA CTTCAACCCA GAGGGACAAA CAAGTTCCAA 
TTCTTAATTC CTGAGCAGGT CCAGATCAAC CTGCCACACT ACATCCCCTT GAAGGAGAAG 
GAGGTCATGG CTGGCCAGAC CATTTCCCTG GTTCCCCCCA AGCTTCTGAA TGCCTGGAAG 
GGCCATTTGG ATAGTGTGGC AGACATCCTG TATGTGGATA GCTTCCAGCT GATTATCAGT 
GCTGGCCACG ATTGTGACGT CAAGGCTTGG AAACTCTCCG GTGACACCAT TGGGACGTTT 
GGCGTGAGTG TTTGGAAAGG GTTGCAGGAT ATTCAGACGG TTGGTGATCT TGCACTAAGA 
AAAAGCTCAG AGGAGGAGGC TGACTCTATA GATGCTGCCC AGAAGGCCTT CCGTCCAGAG 
CTGCAGGAGG AGCGGGCTCT TGCCGAGGCC CTGGTGTACC AGCGGCGGGA GCAGGTGGCA 
CTCATGGCTC TCCTGAATGG GAAGGCAGAC GCGGAGGCTG AGGCTTGGGG TAAGCTGCAG 
AGGATAACCC AGATGTCCCC ATGGGCTGGA GAGCACTACT CAGAAGACGT TGAGCACAGC 
TGGCTCGAGT GGGAGTCCAA GGGCAAGCAG GTGAGCAAAA TCGTGGGAGG AGCATACAAG 
GCCAAGGACC GGTCTCGGAG TCCCGGAGTC CTGTCCACTA ATGTGCAGTA TGGCTGGATG 
AAGCACCAGA TTTCACCTCA GATCTACCAA AGCCTGCACT TCAACCAGCT GTCACCCACC 
CACACACCTG ACTTCCTGGT GCACAATATC CTAGGGCATC ACGGCCAGCA CATCTACCTG 
GTGACCCACA ATAGCCAAAA GGACCTGGAT CCCGAAGAGA CCAACTCGTC TACTGCCTCT 
GTCTCCCCCA GCGACACCCT CACCCCGAAA GCCTCAGGCT CCGGGTTCCT GGAGTCCGCC 
TTGTCCACCT CCCCGTACCT CCAGCCCTCC CTCTCCCTCC TGTCCCAGCC ATCCAGGTAT 
GTCACGCAGA GGTCGGCGCC CCCCATGCCT GTAGCCTCAT CTATCAAACT GCTCTCAAGA 
CCTCTGAAGG CCGCCCTTTC ATCCTCCATG AAGAAGAGCC ACGGCGTCAG GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107224.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962699.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe42872
Clone ID Related Accession (Same CDS sequence)	XM_014107224.2 , XM_014107224.1
Accession Version	XM_014107224.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107224.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGATTGG GATCCTAAAA GGGTACTTGG AGACTGAGGG GCCTGGGAAA 
CCAAGGGAGA GGACCACAAC TTATAGCACA CCCCTGTGCG CTGTTCTCTA CAGCAAGATT 
TTTAAGCAGG TGGTAAGCGG CTGCCTGAGC AGCTTGGTGA GTGTGTGGGA GATCAAGACA 
GGCAAGAGGA TGATGGAGTT CTCTGTGACT GGCGACCAGC AAGTGGAGCT GACCACCATG 
TGCCTGGATG AATCCGAGCG GTGCCTGCTC ACGGGTTTGC GCGACGGCAC CATAAAGATG 
TGGAACTACA CTGTGGGTGA ATGCCTGCTG ACCTTCCCCA ACCCGGACCA GCTGGAGATT 
AGCGGGATTG TCCATATGAA CAAAGTGTTC TACGTGACTG GATGGAGTAA GAGAATCACC 
TGTTTCATGC TCCATAAGAC CAAGCCAGTC CTCCTGTGCT ACCAATGGCA GAGCTTCCAC 
AAGGAAGATG TTCTGAGCAT GGCCAAGTAC CAAAACCAGT TCCTTGGGAC CTCTTCCTAC 
AATGGAGATA TTCTCTTCTG GAACATCAAC CTGTTCAAGC CCATCCTCAA TTTCAATGCT 
TCTAAGAGCC CCTGGCCTTT GCTGCCCAAG AAGATCCAAG AAGTGGACAA CTCCCTGGCT 
GAGAGCCTCC AGTCCAGCAA GCTTTGTGTG AAACATAAGG AGTGGGCGTA CAAACCATCA 
ACGCAGCCCC TGGGCCCCAG AGCCAGGGCT GTAGCCAATG CCAACCTGCA ACGGAACCTG 
ATGTCAGCTC CCCCAGTGAT GAAGCGCCCC AGAGACAAGG AGCCAGAAAA GCCCATGCCC 
CCGCAGGTGG GAGCGGAGGT GAGGCAGAAA CATGTCAGTT CACAAAGCCC CAAGCTGTTA 
AGAATGCCCC ATGGCAAACA GTCCATCATC AGAGACGCTC AAAGGAAAAG AGGGGAATTC 
CAGAAGAAGA TACTACTGCA ATCCAACACA TCGGTGGAGA AGATAATCTT CCTGCAGACC 
AGGCCTCGCC TGCCACACAT AGCTGCCCTG CTGAGCAGCT GCATCGATGG CTACATCTAT 
GCCTGGTCCA TCCACGGGAG CGGAGGCCTG TTGGGGAAGT TCCCTGTGGA CTTCAAAAAC 
CAGGGGGATG TGGGTGTGGG TGCCATGGCC ACGGATGAGA AAGACTTGAT CCTTGTCACA 
GGAGATTGTA AAGGGCACAT CAAGATTTGG GACATCGAGG ATTATTGCAT GTTCACCGAC 
AAGACACCAC TTCAACCCAG AGGGACAAAC AAGTTCCAAT TCTTAATTCC TGAGCAGGTC 
CAGATCAACC TGCCACACTA CATCCCCTTG AAGGAGAAGG AGGTCATGGC TGGCCAGACC 
ATTTCCCTGG TTCCCCCCAA GCTTCTGAAT GCCTGGAAGG GCCATTTGGA TAGTGTGGCA 
GACATCCTGT ATGTGGATAG CTTCCAGCTG ATTATCAGTG CTGGCCACGA TTGTGACGTC 
AAGGCTTGGA AACTCTCCGG TGACACCATT GGGACGTTTG GCGTGAGTGT TTGGAAAGGG 
TTGCAGGATA TTCAGACGGT TGGTGATCTT GCACTAAGAA AAAGCTCAGA GGAGGAGGCT 
GACTCTATAG ATGCTGCCCA GAAGGCCTTC CGTCCAGAGC TGCAGGAGGA GCGGGCTCTT 
GCCGAGGCCC TGGTGTACCA GCGGCGGGAG CAGGTGGCAC TCATGGCTCT CCTGAATGGG 
AAGGCAGACG CGGAGGCTGA GGCTTGGGGT AAGCTGCAGA GGATAACCCA GATGTCCCCA 
TGGGCTGGAG AGCACTACTC AGAAGACGTT GAGCACAGCT GGCTCGAGTG GGAGTCCAAG 
GGCAAGCAGG TGAGCAAAAT CGTGGGAGGA GCATACAAGG CCAAGGACCG GTCTCGGAGT 
CCCGGAGTCC TGTCCACTAA TGTGCAGTAT GGCTGGATGA AGCACCAGAT TTCACCTCAG 
ATCTACCAAA GCCTGCACTT CAACCAGCTG TCACCCACCC ACACACCTGA CTTCCTGGTG 
CACAATATCC TAGGGCATCA CGGCCAGCAC ATCTACCTGG TGACCCACAA TAGCCAAAAG 
GACCTGGATC CCGAAGAGAC CAACTCGTCT ACTGCCTCTG TCTCCCCCAG CGACACCCTC 
ACCCCGAAAG CCTCAGGCTC CGGGTTCCTG GAGTCCGCCT TGTCCACCTC CCCGTACCTC 
CAGCCCTCCC TCTCCCTCCT GTCCCAGCCA TCCAGGTATG TCACGCAGAG GTCGGCGCCC 
CCCATGCCTG TAGCCTCATC TATCAAACTG CTCTCAAGAC CTCTGAAGGC CGCCCTTTCA 
TCCTCCATGA AGAAGAGCCA CGGCGTCAGG TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962699.1
CDS	1140..4895
Translation	MSQLRWKSKTNIFLSKIASKVTWEEQLGSGSKPSSSEDLRESPQSQEASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGALDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107224.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107224.2

ORF Insert Sequence:

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGATTGG GATCCTAAAA GGGTACTTGG AGACTGAGGG GCCTGGGAAA 
CCAAGGGAGA GGACCACAAC TTATAGCACA CCCCTGTGCG CTGTTCTCTA CAGCAAGATT 
TTTAAGCAGG TGGTAAGCGG CTGCCTGAGC AGCTTGGTGA GTGTGTGGGA GATCAAGACA 
GGCAAGAGGA TGATGGAGTT CTCTGTGACT GGCGACCAGC AAGTGGAGCT GACCACCATG 
TGCCTGGATG AATCCGAGCG GTGCCTGCTC ACGGGTTTGC GCGACGGCAC CATAAAGATG 
TGGAACTACA CTGTGGGTGA ATGCCTGCTG ACCTTCCCCA ACCCGGACCA GCTGGAGATT 
AGCGGGATTG TCCATATGAA CAAAGTGTTC TACGTGACTG GATGGAGTAA GAGAATCACC 
TGTTTCATGC TCCATAAGAC CAAGCCAGTC CTCCTGTGCT ACCAATGGCA GAGCTTCCAC 
AAGGAAGATG TTCTGAGCAT GGCCAAGTAC CAAAACCAGT TCCTTGGGAC CTCTTCCTAC 
AATGGAGATA TTCTCTTCTG GAACATCAAC CTGTTCAAGC CCATCCTCAA TTTCAATGCT 
TCTAAGAGCC CCTGGCCTTT GCTGCCCAAG AAGATCCAAG AAGTGGACAA CTCCCTGGCT 
GAGAGCCTCC AGTCCAGCAA GCTTTGTGTG AAACATAAGG AGTGGGCGTA CAAACCATCA 
ACGCAGCCCC TGGGCCCCAG AGCCAGGGCT GTAGCCAATG CCAACCTGCA ACGGAACCTG 
ATGTCAGCTC CCCCAGTGAT GAAGCGCCCC AGAGACAAGG AGCCAGAAAA GCCCATGCCC 
CCGCAGGTGG GAGCGGAGGT GAGGCAGAAA CATGTCAGTT CACAAAGCCC CAAGCTGTTA 
AGAATGCCCC ATGGCAAACA GTCCATCATC AGAGACGCTC AAAGGAAAAG AGGGGAATTC 
CAGAAGAAGA TACTACTGCA ATCCAACACA TCGGTGGAGA AGATAATCTT CCTGCAGACC 
AGGCCTCGCC TGCCACACAT AGCTGCCCTG CTGAGCAGCT GCATCGATGG CTACATCTAT 
GCCTGGTCCA TCCACGGGAG CGGAGGCCTG TTGGGGAAGT TCCCTGTGGA CTTCAAAAAC 
CAGGGGGATG TGGGTGTGGG TGCCATGGCC ACGGATGAGA AAGACTTGAT CCTTGTCACA 
GGAGATTGTA AAGGGCACAT CAAGATTTGG GACATCGAGG ATTATTGCAT GTTCACCGAC 
AAGACACCAC TTCAACCCAG AGGGACAAAC AAGTTCCAAT TCTTAATTCC TGAGCAGGTC 
CAGATCAACC TGCCACACTA CATCCCCTTG AAGGAGAAGG AGGTCATGGC TGGCCAGACC 
ATTTCCCTGG TTCCCCCCAA GCTTCTGAAT GCCTGGAAGG GCCATTTGGA TAGTGTGGCA 
GACATCCTGT ATGTGGATAG CTTCCAGCTG ATTATCAGTG CTGGCCACGA TTGTGACGTC 
AAGGCTTGGA AACTCTCCGG TGACACCATT GGGACGTTTG GCGTGAGTGT TTGGAAAGGG 
TTGCAGGATA TTCAGACGGT TGGTGATCTT GCACTAAGAA AAAGCTCAGA GGAGGAGGCT 
GACTCTATAG ATGCTGCCCA GAAGGCCTTC CGTCCAGAGC TGCAGGAGGA GCGGGCTCTT 
GCCGAGGCCC TGGTGTACCA GCGGCGGGAG CAGGTGGCAC TCATGGCTCT CCTGAATGGG 
AAGGCAGACG CGGAGGCTGA GGCTTGGGGT AAGCTGCAGA GGATAACCCA GATGTCCCCA 
TGGGCTGGAG AGCACTACTC AGAAGACGTT GAGCACAGCT GGCTCGAGTG GGAGTCCAAG 
GGCAAGCAGG TGAGCAAAAT CGTGGGAGGA GCATACAAGG CCAAGGACCG GTCTCGGAGT 
CCCGGAGTCC TGTCCACTAA TGTGCAGTAT GGCTGGATGA AGCACCAGAT TTCACCTCAG 
ATCTACCAAA GCCTGCACTT CAACCAGCTG TCACCCACCC ACACACCTGA CTTCCTGGTG 
CACAATATCC TAGGGCATCA CGGCCAGCAC ATCTACCTGG TGACCCACAA TAGCCAAAAG 
GACCTGGATC CCGAAGAGAC CAACTCGTCT ACTGCCTCTG TCTCCCCCAG CGACACCCTC 
ACCCCGAAAG CCTCAGGCTC CGGGTTCCTG GAGTCCGCCT TGTCCACCTC CCCGTACCTC 
CAGCCCTCCC TCTCCCTCCT GTCCCAGCCA TCCAGGTATG TCACGCAGAG GTCGGCGCCC 
CCCATGCCTG TAGCCTCATC TATCAAACTG CTCTCAAGAC CTCTGAAGGC CGCCCTTTCA 
TCCTCCATGA AGAAGAGCCA CGGCGTCAGG TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107224.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962699.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X5, mRNA.

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CloneID	OCe42872
Clone ID Related Accession (Same CDS sequence)	XM_014107224.2 , XM_014107224.1
Accession Version	XM_014107224.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGATTGG GATCCTAAAA GGGTACTTGG AGACTGAGGG GCCTGGGAAA 
CCAAGGGAGA GGACCACAAC TTATAGCACA CCCCTGTGCG CTGTTCTCTA CAGCAAGATT 
TTTAAGCAGG TGGTAAGCGG CTGCCTGAGC AGCTTGGTGA GTGTGTGGGA GATCAAGACA 
GGCAAGAGGA TGATGGAGTT CTCTGTGACT GGCGACCAGC AAGTGGAGCT GACCACCATG 
TGCCTGGATG AATCCGAGCG GTGCCTGCTC ACGGGTTTGC GCGACGGCAC CATAAAGATG 
TGGAACTACA CTGTGGGTGA ATGCCTGCTG ACCTTCCCCA ACCCGGACCA GCTGGAGATT 
AGCGGGATTG TCCATATGAA CAAAGTGTTC TACGTGACTG GATGGAGTAA GAGAATCACC 
TGTTTCATGC TCCATAAGAC CAAGCCAGTC CTCCTGTGCT ACCAATGGCA GAGCTTCCAC 
AAGGAAGATG TTCTGAGCAT GGCCAAGTAC CAAAACCAGT TCCTTGGGAC CTCTTCCTAC 
AATGGAGATA TTCTCTTCTG GAACATCAAC CTGTTCAAGC CCATCCTCAA TTTCAATGCT 
TCTAAGAGCC CCTGGCCTTT GCTGCCCAAG AAGATCCAAG AAGTGGACAA CTCCCTGGCT 
GAGAGCCTCC AGTCCAGCAA GCTTTGTGTG AAACATAAGG AGTGGGCGTA CAAACCATCA 
ACGCAGCCCC TGGGCCCCAG AGCCAGGGCT GTAGCCAATG CCAACCTGCA ACGGAACCTG 
ATGTCAGCTC CCCCAGTGAT GAAGCGCCCC AGAGACAAGG AGCCAGAAAA GCCCATGCCC 
CCGCAGGTGG GAGCGGAGGT GAGGCAGAAA CATGTCAGTT CACAAAGCCC CAAGCTGTTA 
AGAATGCCCC ATGGCAAACA GTCCATCATC AGAGACGCTC AAAGGAAAAG AGGGGAATTC 
CAGAAGAAGA TACTACTGCA ATCCAACACA TCGGTGGAGA AGATAATCTT CCTGCAGACC 
AGGCCTCGCC TGCCACACAT AGCTGCCCTG CTGAGCAGCT GCATCGATGG CTACATCTAT 
GCCTGGTCCA TCCACGGGAG CGGAGGCCTG TTGGGGAAGT TCCCTGTGGA CTTCAAAAAC 
CAGGGGGATG TGGGTGTGGG TGCCATGGCC ACGGATGAGA AAGACTTGAT CCTTGTCACA 
GGAGATTGTA AAGGGCACAT CAAGATTTGG GACATCGAGG ATTATTGCAT GTTCACCGAC 
AAGACACCAC TTCAACCCAG AGGGACAAAC AAGTTCCAAT TCTTAATTCC TGAGCAGGTC 
CAGATCAACC TGCCACACTA CATCCCCTTG AAGGAGAAGG AGGTCATGGC TGGCCAGACC 
ATTTCCCTGG TTCCCCCCAA GCTTCTGAAT GCCTGGAAGG GCCATTTGGA TAGTGTGGCA 
GACATCCTGT ATGTGGATAG CTTCCAGCTG ATTATCAGTG CTGGCCACGA TTGTGACGTC 
AAGGCTTGGA AACTCTCCGG TGACACCATT GGGACGTTTG GCGTGAGTGT TTGGAAAGGG 
TTGCAGGATA TTCAGACGGT TGGTGATCTT GCACTAAGAA AAAGCTCAGA GGAGGAGGCT 
GACTCTATAG ATGCTGCCCA GAAGGCCTTC CGTCCAGAGC TGCAGGAGGA GCGGGCTCTT 
GCCGAGGCCC TGGTGTACCA GCGGCGGGAG CAGGTGGCAC TCATGGCTCT CCTGAATGGG 
AAGGCAGACG CGGAGGCTGA GGCTTGGGGT AAGCTGCAGA GGATAACCCA GATGTCCCCA 
TGGGCTGGAG AGCACTACTC AGAAGACGTT GAGCACAGCT GGCTCGAGTG GGAGTCCAAG 
GGCAAGCAGG TGAGCAAAAT CGTGGGAGGA GCATACAAGG CCAAGGACCG GTCTCGGAGT 
CCCGGAGTCC TGTCCACTAA TGTGCAGTAT GGCTGGATGA AGCACCAGAT TTCACCTCAG 
ATCTACCAAA GCCTGCACTT CAACCAGCTG TCACCCACCC ACACACCTGA CTTCCTGGTG 
CACAATATCC TAGGGCATCA CGGCCAGCAC ATCTACCTGG TGACCCACAA TAGCCAAAAG 
GACCTGGATC CCGAAGAGAC CAACTCGTCT ACTGCCTCTG TCTCCCCCAG CGACACCCTC 
ACCCCGAAAG CCTCAGGCTC CGGGTTCCTG GAGTCCGCCT TGTCCACCTC CCCGTACCTC 
CAGCCCTCCC TCTCCCTCCT GTCCCAGCCA TCCAGGTATG TCACGCAGAG GTCGGCGCCC 
CCCATGCCTG TAGCCTCATC TATCAAACTG CTCTCAAGAC CTCTGAAGGC CGCCCTTTCA 
TCCTCCATGA AGAAGAGCCA CGGCGTCAGG TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962699.1
CDS	1391..5146
Translation	MSQLRWKSKTNIFLSKIASKVTWEEQLGSGSKPSSSEDLRESPQSQEASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGALDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107224.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107224.1

ORF Insert Sequence:

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGATTGG GATCCTAAAA GGGTACTTGG AGACTGAGGG GCCTGGGAAA 
CCAAGGGAGA GGACCACAAC TTATAGCACA CCCCTGTGCG CTGTTCTCTA CAGCAAGATT 
TTTAAGCAGG TGGTAAGCGG CTGCCTGAGC AGCTTGGTGA GTGTGTGGGA GATCAAGACA 
GGCAAGAGGA TGATGGAGTT CTCTGTGACT GGCGACCAGC AAGTGGAGCT GACCACCATG 
TGCCTGGATG AATCCGAGCG GTGCCTGCTC ACGGGTTTGC GCGACGGCAC CATAAAGATG 
TGGAACTACA CTGTGGGTGA ATGCCTGCTG ACCTTCCCCA ACCCGGACCA GCTGGAGATT 
AGCGGGATTG TCCATATGAA CAAAGTGTTC TACGTGACTG GATGGAGTAA GAGAATCACC 
TGTTTCATGC TCCATAAGAC CAAGCCAGTC CTCCTGTGCT ACCAATGGCA GAGCTTCCAC 
AAGGAAGATG TTCTGAGCAT GGCCAAGTAC CAAAACCAGT TCCTTGGGAC CTCTTCCTAC 
AATGGAGATA TTCTCTTCTG GAACATCAAC CTGTTCAAGC CCATCCTCAA TTTCAATGCT 
TCTAAGAGCC CCTGGCCTTT GCTGCCCAAG AAGATCCAAG AAGTGGACAA CTCCCTGGCT 
GAGAGCCTCC AGTCCAGCAA GCTTTGTGTG AAACATAAGG AGTGGGCGTA CAAACCATCA 
ACGCAGCCCC TGGGCCCCAG AGCCAGGGCT GTAGCCAATG CCAACCTGCA ACGGAACCTG 
ATGTCAGCTC CCCCAGTGAT GAAGCGCCCC AGAGACAAGG AGCCAGAAAA GCCCATGCCC 
CCGCAGGTGG GAGCGGAGGT GAGGCAGAAA CATGTCAGTT CACAAAGCCC CAAGCTGTTA 
AGAATGCCCC ATGGCAAACA GTCCATCATC AGAGACGCTC AAAGGAAAAG AGGGGAATTC 
CAGAAGAAGA TACTACTGCA ATCCAACACA TCGGTGGAGA AGATAATCTT CCTGCAGACC 
AGGCCTCGCC TGCCACACAT AGCTGCCCTG CTGAGCAGCT GCATCGATGG CTACATCTAT 
GCCTGGTCCA TCCACGGGAG CGGAGGCCTG TTGGGGAAGT TCCCTGTGGA CTTCAAAAAC 
CAGGGGGATG TGGGTGTGGG TGCCATGGCC ACGGATGAGA AAGACTTGAT CCTTGTCACA 
GGAGATTGTA AAGGGCACAT CAAGATTTGG GACATCGAGG ATTATTGCAT GTTCACCGAC 
AAGACACCAC TTCAACCCAG AGGGACAAAC AAGTTCCAAT TCTTAATTCC TGAGCAGGTC 
CAGATCAACC TGCCACACTA CATCCCCTTG AAGGAGAAGG AGGTCATGGC TGGCCAGACC 
ATTTCCCTGG TTCCCCCCAA GCTTCTGAAT GCCTGGAAGG GCCATTTGGA TAGTGTGGCA 
GACATCCTGT ATGTGGATAG CTTCCAGCTG ATTATCAGTG CTGGCCACGA TTGTGACGTC 
AAGGCTTGGA AACTCTCCGG TGACACCATT GGGACGTTTG GCGTGAGTGT TTGGAAAGGG 
TTGCAGGATA TTCAGACGGT TGGTGATCTT GCACTAAGAA AAAGCTCAGA GGAGGAGGCT 
GACTCTATAG ATGCTGCCCA GAAGGCCTTC CGTCCAGAGC TGCAGGAGGA GCGGGCTCTT 
GCCGAGGCCC TGGTGTACCA GCGGCGGGAG CAGGTGGCAC TCATGGCTCT CCTGAATGGG 
AAGGCAGACG CGGAGGCTGA GGCTTGGGGT AAGCTGCAGA GGATAACCCA GATGTCCCCA 
TGGGCTGGAG AGCACTACTC AGAAGACGTT GAGCACAGCT GGCTCGAGTG GGAGTCCAAG 
GGCAAGCAGG TGAGCAAAAT CGTGGGAGGA GCATACAAGG CCAAGGACCG GTCTCGGAGT 
CCCGGAGTCC TGTCCACTAA TGTGCAGTAT GGCTGGATGA AGCACCAGAT TTCACCTCAG 
ATCTACCAAA GCCTGCACTT CAACCAGCTG TCACCCACCC ACACACCTGA CTTCCTGGTG 
CACAATATCC TAGGGCATCA CGGCCAGCAC ATCTACCTGG TGACCCACAA TAGCCAAAAG 
GACCTGGATC CCGAAGAGAC CAACTCGTCT ACTGCCTCTG TCTCCCCCAG CGACACCCTC 
ACCCCGAAAG CCTCAGGCTC CGGGTTCCTG GAGTCCGCCT TGTCCACCTC CCCGTACCTC 
CAGCCCTCCC TCTCCCTCCT GTCCCAGCCA TCCAGGTATG TCACGCAGAG GTCGGCGCCC 
CCCATGCCTG TAGCCTCATC TATCAAACTG CTCTCAAGAC CTCTGAAGGC CGCCCTTTCA 
TCCTCCATGA AGAAGAGCCA CGGCGTCAGG TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107225.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962700.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X6, mRNA.

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CloneID	OCe42873
Clone ID Related Accession (Same CDS sequence)	XM_014107225.1
Accession Version	XM_014107225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTCA CCAACATGGT GGATTTCAAG GCTCAGAGTA GCCATGAGAT AGCAGCTCCA 
GCAAGATGTG TGCTACAATC TTCCCTGGAC ATGAAGACTT TCATTAAGGC CATAAAGAAG 
ATTCTGAGCA ATGCGTCAGA TGAGATGCTA GAGGCGCTGT TTTTGAAGGT GGACACAGAC 
TGTAATGGCT CCATTACCTG GCAGGAGTAC GTGGATTACG TGATGCGGGA GTTACAGAGA 
AGGGAGAGGA TGAGAACAAG CCAGTACCGC CTGCACTTCC ACCTTCCCAT GAGGATCATT 
CCCCTGAACC ATGGATGCGA GATTGTGAAG GTGCAGTTCT TAACTCAGCG GTTCAAGAAG 
ATGGGGCACC TTCTGACTGT CACCAAGGAC GGGATCTTGC AGATCTGGTC TGAATCCTTC 
TCGCTGCTTA ACTCCTTTAG GCTTAACCAG ATCCATCAGC CCTATAACCA GCAGATGTGG 
GTCACTGATA TGGTGTGTCT CCATAACATG AACCTGATTG CGATTGCATC AACTGACCAG 
AAAATAGAGT TCTTTGATAT TACCAACCAT AATTGTGCCC GGGCCTTCAC CTTCATTGAT 
CTGGACAGCT GTGTCCTGAC CATGGACTAT TGGTCCGACT ATCACAGAGG TGTATTCTGC 
TACGGGGACA CCAAAGGCAA TGTCATTGTC TTCACCTCTG ACAATGTGAC CCACGGGCTG 
TTCAACCCTC GAATCCTTCC CAGGACCTCC AAATGGGATC ACTGGACCAA AGTTTCTACA 
CGGAAGCTTC TAAATGAGAA GTCTCCTTTG TACAGGAGTT ATTGGCTAAA GGCTCTCCAT 
CCCAACTGGT GCCAACAGGT CAAGTTCATT CCTCAGCTGA ACACAGTGGC CTCCTGTTCG 
GCCATTGAGA AGTCCTCTCT GGTGCTAATA GCATTGCCAT CTAAAGCCCC AGAGAGCCCC 
AAGTTTTCAG TGCTGAACTT AAGAAAAGGG ATTCTTTGCT TCGATTACTG CCCAGACAAG 
AACTTAGTGG TGACTGGTGG CTATGACCCC CTCATCCGCC TGTGGAACCC CTTCTTCTCA 
AAAAAGCCTG TGTGGATGAT GAAGGGGCAT CAGACTTCCG TGACGCACAT CCTCGTGAAC 
AGCAAGAACA GCAGCATCCT CCTCAGCATC TCCAAGGACA AGAATATCCG TGTGTGGGAC 
ATGCAGGACT ACATATGCCT CCAGTCCTTT TCCGGGAAGC TTTTTGCCCT GGGAAACTGC 
CCCATCACCA GTGTCTACTT CCACAAGAAT GAAGACAGTC TCATCTGTAG CACCTTTTCA 
ATTGGGATCC TAAAAGGGTA CTTGGAGACT GAGGGGCCTG GGAAACCAAG GGAGAGGACC 
ACAACTTATA GCACACCCCT GTGCGCTGTT CTCTACAGCA AGATTTTTAA GCAGGTGGTA 
AGCGGCTGCC TGAGCAGCTT GGTGAGTGTG TGGGAGATCA AGACAGGCAA GAGGATGATG 
GAGTTCTCTG TGACTGGCGA CCAGCAAGTG GAGCTGACCA CCATGTGCCT GGATGAATCC 
GAGCGGTGCC TGCTCACGGG TTTGCGCGAC GGCACCATAA AGATGTGGAA CTACACTGTG 
GGTGAATGCC TGCTGACCTT CCCCAACCCG GACCAGCTGG AGATTAGCGG GATTGTCCAT 
ATGAACAAAG TGTTCTACGT GACTGGATGG AGTAAGAGAA TCACCTGTTT CATGCTCCAT 
AAGACCAAGC CAGTCCTCCT GTGCTACCAA TGGCAGAGCT TCCACAAGGA AGATGTTCTG 
AGCATGGCCA AGTACCAAAA CCAGTTCCTT GGGACCTCTT CCTACAATGG AGATATTCTC 
TTCTGGAACA TCAACCTGTT CAAGCCCATC CTCAATTTCA ATGCTTCTAA GAGCCCCTGG 
CCTTTGCTGC CCAAGAAGAT CCAAGAAGTG GACAACTCCC TGGCTGAGAG CCTCCAGTCC 
AGCAAGCTTT GTGTGAAACA TAAGGAGTGG GCGTACAAAC CATCAACGCA GCCCCTGGGC 
CCCAGAGCCA GGGCTGTAGC CAATGCCAAC CTGCAACGGA ACCTGATGTC AGCTCCCCCA 
GTGATGAAGC GCCCCAGAGA CAAGGAGCCA GAAAAGCCCA TGCCCCCGCA GGTGGGAGCG 
GAGGTGAGGC AGAAACATGT CAGTTCACAA AGCCCCAAGC TGTTAAGAAT GCCCCATGGC 
AAACAGTCCA TCATCAGAGA CGCTCAAAGG AAAAGAGGGG AATTCCAGAA GAAGATACTA 
CTGCAATCCA ACACATCGGT GGAGAAGATA ATCTTCCTGC AGACCAGGCC TCGCCTGCCA 
CACATAGCTG CCCTGCTGAG CAGCTGCATC GATGGCTACA TCTATGCCTG GTCCATCCAC 
GGGAGCGGAG GCCTGTTGGG GAAGTTCCCT GTGGACTTCA AAAACCAGGG GGATGTGGGT 
GTGGGTGCCA TGGCCACGGA TGAGAAAGAC TTGATCCTTG TCACAGGAGA TTGTAAAGGG 
CACATCAAGA TTTGGGACAT CGAGGATTAT TGCATGTTCA CCGACAAGAC ACCACTTCAA 
CCCAGAGGGA CAAACAAGTT CCAATTCTTA ATTCCTGAGC AGGTCCAGAT CAACCTGCCA 
CACTACATCC CCTTGAAGGA GAAGGAGGTC ATGGCTGGCC AGACCATTTC CCTGGTTCCC 
CCCAAGCTTC TGAATGCCTG GAAGGGCCAT TTGGATAGTG TGGCAGACAT CCTGTATGTG 
GATAGCTTCC AGCTGATTAT CAGTGCTGGC CACGATTGTG ACGTCAAGGC TTGGAAACTC 
TCCGGTGACA CCATTGGGAC GTTTGGCGTG AGTGTTTGGA AAGGGTTGCA GGATATTCAG 
ACGGTTGGTG ATCTTGCACT AAGAAAAAGC TCAGAGGAGG AGGCTGACTC TATAGATGCT 
GCCCAGAAGG CCTTCCGTCC AGAGCTGCAG GAGGAGCGGG CTCTTGCCGA GGCCCTGGTG 
TACCAGCGGC GGGAGCAGGT GGCACTCATG GCTCTCCTGA ATGGGAAGGC AGACGCGGAG 
GCTGAGGCTT GGGGTAAGCT GCAGAGGATA ACCCAGATGT CCCCATGGGC TGGAGAGCAC 
TACTCAGAAG ACGTTGAGCA CAGCTGGCTC GAGTGGGAGT CCAAGGGCAA GCAGGTGAGC 
AAAATCGTGG GAGGAGCATA CAAGGCCAAG GACCGGTCTC GGAGTCCCGG AGTCCTGTCC 
ACTAATGTGC AGTATGGCTG GATGAAGCAC CAGATTTCAC CTCAGATCTA CCAAAGCCTG 
CACTTCAACC AGCTGTCACC CACCCACACA CCTGACTTCC TGGTGCACAA TATCCTAGGG 
CATCACGGCC AGCACATCTA CCTGGTGACC CACAATAGCC AAAAGGACCT GGATCCCGAA 
GAGACCAACT CGTCTACTGC CTCTGTCTCC CCCAGCGACA CCCTCACCCC GAAAGCCTCA 
GGCTCCGGGT TCCTGGAGTC CGCCTTGTCC ACCTCCCCGT ACCTCCAGCC CTCCCTCTCC 
CTCCTGTCCC AGCCATCCAG GTATGTCACG CAGAGGTCGG CGCCCCCCAT GCCTGTAGCC 
TCATCTATCA AACTGCTCTC AAGACCTCTG AAGGCCGCCC TTTCATCCTC CATGAAGAAG 
AGCCACGGCG TCAGGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962700.1
CDS	3038..6718
Translation	MGLTNMVDFKAQSSHEIAAPARCVLQSSLDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107225.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107225.1

ORF Insert Sequence:

ATGGGACTCA CCAACATGGT GGATTTCAAG GCTCAGAGTA GCCATGAGAT AGCAGCTCCA 
GCAAGATGTG TGCTACAATC TTCCCTGGAC ATGAAGACTT TCATTAAGGC CATAAAGAAG 
ATTCTGAGCA ATGCGTCAGA TGAGATGCTA GAGGCGCTGT TTTTGAAGGT GGACACAGAC 
TGTAATGGCT CCATTACCTG GCAGGAGTAC GTGGATTACG TGATGCGGGA GTTACAGAGA 
AGGGAGAGGA TGAGAACAAG CCAGTACCGC CTGCACTTCC ACCTTCCCAT GAGGATCATT 
CCCCTGAACC ATGGATGCGA GATTGTGAAG GTGCAGTTCT TAACTCAGCG GTTCAAGAAG 
ATGGGGCACC TTCTGACTGT CACCAAGGAC GGGATCTTGC AGATCTGGTC TGAATCCTTC 
TCGCTGCTTA ACTCCTTTAG GCTTAACCAG ATCCATCAGC CCTATAACCA GCAGATGTGG 
GTCACTGATA TGGTGTGTCT CCATAACATG AACCTGATTG CGATTGCATC AACTGACCAG 
AAAATAGAGT TCTTTGATAT TACCAACCAT AATTGTGCCC GGGCCTTCAC CTTCATTGAT 
CTGGACAGCT GTGTCCTGAC CATGGACTAT TGGTCCGACT ATCACAGAGG TGTATTCTGC 
TACGGGGACA CCAAAGGCAA TGTCATTGTC TTCACCTCTG ACAATGTGAC CCACGGGCTG 
TTCAACCCTC GAATCCTTCC CAGGACCTCC AAATGGGATC ACTGGACCAA AGTTTCTACA 
CGGAAGCTTC TAAATGAGAA GTCTCCTTTG TACAGGAGTT ATTGGCTAAA GGCTCTCCAT 
CCCAACTGGT GCCAACAGGT CAAGTTCATT CCTCAGCTGA ACACAGTGGC CTCCTGTTCG 
GCCATTGAGA AGTCCTCTCT GGTGCTAATA GCATTGCCAT CTAAAGCCCC AGAGAGCCCC 
AAGTTTTCAG TGCTGAACTT AAGAAAAGGG ATTCTTTGCT TCGATTACTG CCCAGACAAG 
AACTTAGTGG TGACTGGTGG CTATGACCCC CTCATCCGCC TGTGGAACCC CTTCTTCTCA 
AAAAAGCCTG TGTGGATGAT GAAGGGGCAT CAGACTTCCG TGACGCACAT CCTCGTGAAC 
AGCAAGAACA GCAGCATCCT CCTCAGCATC TCCAAGGACA AGAATATCCG TGTGTGGGAC 
ATGCAGGACT ACATATGCCT CCAGTCCTTT TCCGGGAAGC TTTTTGCCCT GGGAAACTGC 
CCCATCACCA GTGTCTACTT CCACAAGAAT GAAGACAGTC TCATCTGTAG CACCTTTTCA 
ATTGGGATCC TAAAAGGGTA CTTGGAGACT GAGGGGCCTG GGAAACCAAG GGAGAGGACC 
ACAACTTATA GCACACCCCT GTGCGCTGTT CTCTACAGCA AGATTTTTAA GCAGGTGGTA 
AGCGGCTGCC TGAGCAGCTT GGTGAGTGTG TGGGAGATCA AGACAGGCAA GAGGATGATG 
GAGTTCTCTG TGACTGGCGA CCAGCAAGTG GAGCTGACCA CCATGTGCCT GGATGAATCC 
GAGCGGTGCC TGCTCACGGG TTTGCGCGAC GGCACCATAA AGATGTGGAA CTACACTGTG 
GGTGAATGCC TGCTGACCTT CCCCAACCCG GACCAGCTGG AGATTAGCGG GATTGTCCAT 
ATGAACAAAG TGTTCTACGT GACTGGATGG AGTAAGAGAA TCACCTGTTT CATGCTCCAT 
AAGACCAAGC CAGTCCTCCT GTGCTACCAA TGGCAGAGCT TCCACAAGGA AGATGTTCTG 
AGCATGGCCA AGTACCAAAA CCAGTTCCTT GGGACCTCTT CCTACAATGG AGATATTCTC 
TTCTGGAACA TCAACCTGTT CAAGCCCATC CTCAATTTCA ATGCTTCTAA GAGCCCCTGG 
CCTTTGCTGC CCAAGAAGAT CCAAGAAGTG GACAACTCCC TGGCTGAGAG CCTCCAGTCC 
AGCAAGCTTT GTGTGAAACA TAAGGAGTGG GCGTACAAAC CATCAACGCA GCCCCTGGGC 
CCCAGAGCCA GGGCTGTAGC CAATGCCAAC CTGCAACGGA ACCTGATGTC AGCTCCCCCA 
GTGATGAAGC GCCCCAGAGA CAAGGAGCCA GAAAAGCCCA TGCCCCCGCA GGTGGGAGCG 
GAGGTGAGGC AGAAACATGT CAGTTCACAA AGCCCCAAGC TGTTAAGAAT GCCCCATGGC 
AAACAGTCCA TCATCAGAGA CGCTCAAAGG AAAAGAGGGG AATTCCAGAA GAAGATACTA 
CTGCAATCCA ACACATCGGT GGAGAAGATA ATCTTCCTGC AGACCAGGCC TCGCCTGCCA 
CACATAGCTG CCCTGCTGAG CAGCTGCATC GATGGCTACA TCTATGCCTG GTCCATCCAC 
GGGAGCGGAG GCCTGTTGGG GAAGTTCCCT GTGGACTTCA AAAACCAGGG GGATGTGGGT 
GTGGGTGCCA TGGCCACGGA TGAGAAAGAC TTGATCCTTG TCACAGGAGA TTGTAAAGGG 
CACATCAAGA TTTGGGACAT CGAGGATTAT TGCATGTTCA CCGACAAGAC ACCACTTCAA 
CCCAGAGGGA CAAACAAGTT CCAATTCTTA ATTCCTGAGC AGGTCCAGAT CAACCTGCCA 
CACTACATCC CCTTGAAGGA GAAGGAGGTC ATGGCTGGCC AGACCATTTC CCTGGTTCCC 
CCCAAGCTTC TGAATGCCTG GAAGGGCCAT TTGGATAGTG TGGCAGACAT CCTGTATGTG 
GATAGCTTCC AGCTGATTAT CAGTGCTGGC CACGATTGTG ACGTCAAGGC TTGGAAACTC 
TCCGGTGACA CCATTGGGAC GTTTGGCGTG AGTGTTTGGA AAGGGTTGCA GGATATTCAG 
ACGGTTGGTG ATCTTGCACT AAGAAAAAGC TCAGAGGAGG AGGCTGACTC TATAGATGCT 
GCCCAGAAGG CCTTCCGTCC AGAGCTGCAG GAGGAGCGGG CTCTTGCCGA GGCCCTGGTG 
TACCAGCGGC GGGAGCAGGT GGCACTCATG GCTCTCCTGA ATGGGAAGGC AGACGCGGAG 
GCTGAGGCTT GGGGTAAGCT GCAGAGGATA ACCCAGATGT CCCCATGGGC TGGAGAGCAC 
TACTCAGAAG ACGTTGAGCA CAGCTGGCTC GAGTGGGAGT CCAAGGGCAA GCAGGTGAGC 
AAAATCGTGG GAGGAGCATA CAAGGCCAAG GACCGGTCTC GGAGTCCCGG AGTCCTGTCC 
ACTAATGTGC AGTATGGCTG GATGAAGCAC CAGATTTCAC CTCAGATCTA CCAAAGCCTG 
CACTTCAACC AGCTGTCACC CACCCACACA CCTGACTTCC TGGTGCACAA TATCCTAGGG 
CATCACGGCC AGCACATCTA CCTGGTGACC CACAATAGCC AAAAGGACCT GGATCCCGAA 
GAGACCAACT CGTCTACTGC CTCTGTCTCC CCCAGCGACA CCCTCACCCC GAAAGCCTCA 
GGCTCCGGGT TCCTGGAGTC CGCCTTGTCC ACCTCCCCGT ACCTCCAGCC CTCCCTCTCC 
CTCCTGTCCC AGCCATCCAG GTATGTCACG CAGAGGTCGG CGCCCCCCAT GCCTGTAGCC 
TCATCTATCA AACTGCTCTC AAGACCTCTG AAGGCCGCCC TTTCATCCTC CATGAAGAAG 
AGCCACGGCG TCAGGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107226.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962701.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X7, mRNA.

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CloneID	OCe42874
Clone ID Related Accession (Same CDS sequence)	XM_014107226.2 , XM_014107226.1
Accession Version	XM_014107226.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107226.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGATT AGCGGGATTG TCCATATGAA CAAAGTGTTC 
TACGTGACTG GATGGAGTAA GAGAATCACC TGTTTCATGC TCCATAAGAC CAAGCCAGTC 
CTCCTGTGCT ACCAATGGCA GAGCTTCCAC AAGGAAGATG TTCTGAGCAT GGCCAAGTAC 
CAAAACCAGT TCCTTGGGAC CTCTTCCTAC AATGGAGATA TTCTCTTCTG GAACATCAAC 
CTGTTCAAGC CCATCCTCAA TTTCAATGCT TCTAAGAGCC CCTGGCCTTT GCTGCCCAAG 
AAGATCCAAG AAGTGGACAA CTCCCTGGCT GAGAGCCTCC AGTCCAGCAA GCTTTGTGTG 
AAACATAAGG AGTGGGCGTA CAAACCATCA ACGCAGCCCC TGGGCCCCAG AGCCAGGGCT 
GTAGCCAATG CCAACCTGCA ACGGAACCTG ATGTCAGCTC CCCCAGTGAT GAAGCGCCCC 
AGAGACAAGG AGCCAGAAAA GCCCATGCCC CCGCAGGTGG GAGCGGAGGT GAGGCAGAAA 
CATGTCAGTT CACAAAGCCC CAAGCTGTTA AGAATGCCCC ATGGCAAACA GTCCATCATC 
AGAGACGCTC AAAGGAAAAG AGGGGAATTC CAGAAGAAGA TACTACTGCA ATCCAACACA 
TCGGTGGAGA AGATAATCTT CCTGCAGACC AGGCCTCGCC TGCCACACAT AGCTGCCCTG 
CTGAGCAGCT GCATCGATGG CTACATCTAT GCCTGGTCCA TCCACGGGAG CGGAGGCCTG 
TTGGGGAAGT TCCCTGTGGA CTTCAAAAAC CAGGGGGATG TGGGTGTGGG TGCCATGGCC 
ACGGATGAGA AAGACTTGAT CCTTGTCACA GGAGATTGTA AAGGGCACAT CAAGATTTGG 
GACATCGAGG ATTATTGCAT GTTCACCGAC AAGACACCAC TTCAACCCAG AGGGACAAAC 
AAGTTCCAAT TCTTAATTCC TGAGCAGGTC CAGATCAACC TGCCACACTA CATCCCCTTG 
AAGGAGAAGG AGGTCATGGC TGGCCAGACC ATTTCCCTGG TTCCCCCCAA GCTTCTGAAT 
GCCTGGAAGG GCCATTTGGA TAGTGTGGCA GACATCCTGT ATGTGGATAG CTTCCAGCTG 
ATTATCAGTG CTGGCCACGA TTGTGACGTC AAGGCTTGGA AACTCTCCGG TGACACCATT 
GGGACGTTTG GCGTGAGTGT TTGGAAAGGG TTGCAGGATA TTCAGACGGT TGGTGATCTT 
GCACTAAGAA AAAGCTCAGA GGAGGAGGCT GACTCTATAG ATGCTGCCCA GAAGGCCTTC 
CGTCCAGAGC TGCAGGAGGA GCGGGCTCTT GCCGAGGCCC TGGTGTACCA GCGGCGGGAG 
CAGGTGGCAC TCATGGCTCT CCTGAATGGG AAGGCAGACG CGGAGGCTGA GGCTTGGGGT 
AAGCTGCAGA GGATAACCCA GATGTCCCCA TGGGCTGGAG AGCACTACTC AGAAGACGTT 
GAGCACAGCT GGCTCGAGTG GGAGTCCAAG GGCAAGCAGG TGAGCAAAAT CGTGGGAGGA 
GCATACAAGG CCAAGGACCG GTCTCGGAGT CCCGGAGTCC TGTCCACTAA TGTGCAGTAT 
GGCTGGATGA AGCACCAGAT TTCACCTCAG ATCTACCAAA GCCTGCACTT CAACCAGCTG 
TCACCCACCC ACACACCTGA CTTCCTGGTG CACAATATCC TAGGGCATCA CGGCCAGCAC 
ATCTACCTGG TGACCCACAA TAGCCAAAAG GACCTGGATC CCGAAGAGAC CAACTCGTCT 
ACTGCCTCTG TCTCCCCCAG CGACACCCTC ACCCCGAAAG CCTCAGGCTC CGGGTTCCTG 
GAGTCCGCCT TGTCCACCTC CCCGTACCTC CAGCCCTCCC TCTCCCTCCT GTCCCAGCCA 
TCCAGGTATG TCACGCAGAG GTCGGCGCCC CCCATGCCTG TAGCCTCATC TATCAAACTG 
CTCTCAAGAC CTCTGAAGGC CGCCCTTTCA TCCTCCATGA AGAAGAGCCA CGGCGTCAGG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962701.1
CDS	1140..4805
Translation	MSQLRWKSKTNIFLSKIASKVTWEEQLGSGSKPSSSEDLRESPQSQEASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGALDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107226.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107226.2

ORF Insert Sequence:

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGATT AGCGGGATTG TCCATATGAA CAAAGTGTTC 
TACGTGACTG GATGGAGTAA GAGAATCACC TGTTTCATGC TCCATAAGAC CAAGCCAGTC 
CTCCTGTGCT ACCAATGGCA GAGCTTCCAC AAGGAAGATG TTCTGAGCAT GGCCAAGTAC 
CAAAACCAGT TCCTTGGGAC CTCTTCCTAC AATGGAGATA TTCTCTTCTG GAACATCAAC 
CTGTTCAAGC CCATCCTCAA TTTCAATGCT TCTAAGAGCC CCTGGCCTTT GCTGCCCAAG 
AAGATCCAAG AAGTGGACAA CTCCCTGGCT GAGAGCCTCC AGTCCAGCAA GCTTTGTGTG 
AAACATAAGG AGTGGGCGTA CAAACCATCA ACGCAGCCCC TGGGCCCCAG AGCCAGGGCT 
GTAGCCAATG CCAACCTGCA ACGGAACCTG ATGTCAGCTC CCCCAGTGAT GAAGCGCCCC 
AGAGACAAGG AGCCAGAAAA GCCCATGCCC CCGCAGGTGG GAGCGGAGGT GAGGCAGAAA 
CATGTCAGTT CACAAAGCCC CAAGCTGTTA AGAATGCCCC ATGGCAAACA GTCCATCATC 
AGAGACGCTC AAAGGAAAAG AGGGGAATTC CAGAAGAAGA TACTACTGCA ATCCAACACA 
TCGGTGGAGA AGATAATCTT CCTGCAGACC AGGCCTCGCC TGCCACACAT AGCTGCCCTG 
CTGAGCAGCT GCATCGATGG CTACATCTAT GCCTGGTCCA TCCACGGGAG CGGAGGCCTG 
TTGGGGAAGT TCCCTGTGGA CTTCAAAAAC CAGGGGGATG TGGGTGTGGG TGCCATGGCC 
ACGGATGAGA AAGACTTGAT CCTTGTCACA GGAGATTGTA AAGGGCACAT CAAGATTTGG 
GACATCGAGG ATTATTGCAT GTTCACCGAC AAGACACCAC TTCAACCCAG AGGGACAAAC 
AAGTTCCAAT TCTTAATTCC TGAGCAGGTC CAGATCAACC TGCCACACTA CATCCCCTTG 
AAGGAGAAGG AGGTCATGGC TGGCCAGACC ATTTCCCTGG TTCCCCCCAA GCTTCTGAAT 
GCCTGGAAGG GCCATTTGGA TAGTGTGGCA GACATCCTGT ATGTGGATAG CTTCCAGCTG 
ATTATCAGTG CTGGCCACGA TTGTGACGTC AAGGCTTGGA AACTCTCCGG TGACACCATT 
GGGACGTTTG GCGTGAGTGT TTGGAAAGGG TTGCAGGATA TTCAGACGGT TGGTGATCTT 
GCACTAAGAA AAAGCTCAGA GGAGGAGGCT GACTCTATAG ATGCTGCCCA GAAGGCCTTC 
CGTCCAGAGC TGCAGGAGGA GCGGGCTCTT GCCGAGGCCC TGGTGTACCA GCGGCGGGAG 
CAGGTGGCAC TCATGGCTCT CCTGAATGGG AAGGCAGACG CGGAGGCTGA GGCTTGGGGT 
AAGCTGCAGA GGATAACCCA GATGTCCCCA TGGGCTGGAG AGCACTACTC AGAAGACGTT 
GAGCACAGCT GGCTCGAGTG GGAGTCCAAG GGCAAGCAGG TGAGCAAAAT CGTGGGAGGA 
GCATACAAGG CCAAGGACCG GTCTCGGAGT CCCGGAGTCC TGTCCACTAA TGTGCAGTAT 
GGCTGGATGA AGCACCAGAT TTCACCTCAG ATCTACCAAA GCCTGCACTT CAACCAGCTG 
TCACCCACCC ACACACCTGA CTTCCTGGTG CACAATATCC TAGGGCATCA CGGCCAGCAC 
ATCTACCTGG TGACCCACAA TAGCCAAAAG GACCTGGATC CCGAAGAGAC CAACTCGTCT 
ACTGCCTCTG TCTCCCCCAG CGACACCCTC ACCCCGAAAG CCTCAGGCTC CGGGTTCCTG 
GAGTCCGCCT TGTCCACCTC CCCGTACCTC CAGCCCTCCC TCTCCCTCCT GTCCCAGCCA 
TCCAGGTATG TCACGCAGAG GTCGGCGCCC CCCATGCCTG TAGCCTCATC TATCAAACTG 
CTCTCAAGAC CTCTGAAGGC CGCCCTTTCA TCCTCCATGA AGAAGAGCCA CGGCGTCAGG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107226.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962701.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe42874
Clone ID Related Accession (Same CDS sequence)	XM_014107226.2 , XM_014107226.1
Accession Version	XM_014107226.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGATT AGCGGGATTG TCCATATGAA CAAAGTGTTC 
TACGTGACTG GATGGAGTAA GAGAATCACC TGTTTCATGC TCCATAAGAC CAAGCCAGTC 
CTCCTGTGCT ACCAATGGCA GAGCTTCCAC AAGGAAGATG TTCTGAGCAT GGCCAAGTAC 
CAAAACCAGT TCCTTGGGAC CTCTTCCTAC AATGGAGATA TTCTCTTCTG GAACATCAAC 
CTGTTCAAGC CCATCCTCAA TTTCAATGCT TCTAAGAGCC CCTGGCCTTT GCTGCCCAAG 
AAGATCCAAG AAGTGGACAA CTCCCTGGCT GAGAGCCTCC AGTCCAGCAA GCTTTGTGTG 
AAACATAAGG AGTGGGCGTA CAAACCATCA ACGCAGCCCC TGGGCCCCAG AGCCAGGGCT 
GTAGCCAATG CCAACCTGCA ACGGAACCTG ATGTCAGCTC CCCCAGTGAT GAAGCGCCCC 
AGAGACAAGG AGCCAGAAAA GCCCATGCCC CCGCAGGTGG GAGCGGAGGT GAGGCAGAAA 
CATGTCAGTT CACAAAGCCC CAAGCTGTTA AGAATGCCCC ATGGCAAACA GTCCATCATC 
AGAGACGCTC AAAGGAAAAG AGGGGAATTC CAGAAGAAGA TACTACTGCA ATCCAACACA 
TCGGTGGAGA AGATAATCTT CCTGCAGACC AGGCCTCGCC TGCCACACAT AGCTGCCCTG 
CTGAGCAGCT GCATCGATGG CTACATCTAT GCCTGGTCCA TCCACGGGAG CGGAGGCCTG 
TTGGGGAAGT TCCCTGTGGA CTTCAAAAAC CAGGGGGATG TGGGTGTGGG TGCCATGGCC 
ACGGATGAGA AAGACTTGAT CCTTGTCACA GGAGATTGTA AAGGGCACAT CAAGATTTGG 
GACATCGAGG ATTATTGCAT GTTCACCGAC AAGACACCAC TTCAACCCAG AGGGACAAAC 
AAGTTCCAAT TCTTAATTCC TGAGCAGGTC CAGATCAACC TGCCACACTA CATCCCCTTG 
AAGGAGAAGG AGGTCATGGC TGGCCAGACC ATTTCCCTGG TTCCCCCCAA GCTTCTGAAT 
GCCTGGAAGG GCCATTTGGA TAGTGTGGCA GACATCCTGT ATGTGGATAG CTTCCAGCTG 
ATTATCAGTG CTGGCCACGA TTGTGACGTC AAGGCTTGGA AACTCTCCGG TGACACCATT 
GGGACGTTTG GCGTGAGTGT TTGGAAAGGG TTGCAGGATA TTCAGACGGT TGGTGATCTT 
GCACTAAGAA AAAGCTCAGA GGAGGAGGCT GACTCTATAG ATGCTGCCCA GAAGGCCTTC 
CGTCCAGAGC TGCAGGAGGA GCGGGCTCTT GCCGAGGCCC TGGTGTACCA GCGGCGGGAG 
CAGGTGGCAC TCATGGCTCT CCTGAATGGG AAGGCAGACG CGGAGGCTGA GGCTTGGGGT 
AAGCTGCAGA GGATAACCCA GATGTCCCCA TGGGCTGGAG AGCACTACTC AGAAGACGTT 
GAGCACAGCT GGCTCGAGTG GGAGTCCAAG GGCAAGCAGG TGAGCAAAAT CGTGGGAGGA 
GCATACAAGG CCAAGGACCG GTCTCGGAGT CCCGGAGTCC TGTCCACTAA TGTGCAGTAT 
GGCTGGATGA AGCACCAGAT TTCACCTCAG ATCTACCAAA GCCTGCACTT CAACCAGCTG 
TCACCCACCC ACACACCTGA CTTCCTGGTG CACAATATCC TAGGGCATCA CGGCCAGCAC 
ATCTACCTGG TGACCCACAA TAGCCAAAAG GACCTGGATC CCGAAGAGAC CAACTCGTCT 
ACTGCCTCTG TCTCCCCCAG CGACACCCTC ACCCCGAAAG CCTCAGGCTC CGGGTTCCTG 
GAGTCCGCCT TGTCCACCTC CCCGTACCTC CAGCCCTCCC TCTCCCTCCT GTCCCAGCCA 
TCCAGGTATG TCACGCAGAG GTCGGCGCCC CCCATGCCTG TAGCCTCATC TATCAAACTG 
CTCTCAAGAC CTCTGAAGGC CGCCCTTTCA TCCTCCATGA AGAAGAGCCA CGGCGTCAGG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962701.1
CDS	1391..5056
Translation	MSQLRWKSKTNIFLSKIASKVTWEEQLGSGSKPSSSEDLRESPQSQEASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGALDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107226.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107226.1

ORF Insert Sequence:

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGATT AGCGGGATTG TCCATATGAA CAAAGTGTTC 
TACGTGACTG GATGGAGTAA GAGAATCACC TGTTTCATGC TCCATAAGAC CAAGCCAGTC 
CTCCTGTGCT ACCAATGGCA GAGCTTCCAC AAGGAAGATG TTCTGAGCAT GGCCAAGTAC 
CAAAACCAGT TCCTTGGGAC CTCTTCCTAC AATGGAGATA TTCTCTTCTG GAACATCAAC 
CTGTTCAAGC CCATCCTCAA TTTCAATGCT TCTAAGAGCC CCTGGCCTTT GCTGCCCAAG 
AAGATCCAAG AAGTGGACAA CTCCCTGGCT GAGAGCCTCC AGTCCAGCAA GCTTTGTGTG 
AAACATAAGG AGTGGGCGTA CAAACCATCA ACGCAGCCCC TGGGCCCCAG AGCCAGGGCT 
GTAGCCAATG CCAACCTGCA ACGGAACCTG ATGTCAGCTC CCCCAGTGAT GAAGCGCCCC 
AGAGACAAGG AGCCAGAAAA GCCCATGCCC CCGCAGGTGG GAGCGGAGGT GAGGCAGAAA 
CATGTCAGTT CACAAAGCCC CAAGCTGTTA AGAATGCCCC ATGGCAAACA GTCCATCATC 
AGAGACGCTC AAAGGAAAAG AGGGGAATTC CAGAAGAAGA TACTACTGCA ATCCAACACA 
TCGGTGGAGA AGATAATCTT CCTGCAGACC AGGCCTCGCC TGCCACACAT AGCTGCCCTG 
CTGAGCAGCT GCATCGATGG CTACATCTAT GCCTGGTCCA TCCACGGGAG CGGAGGCCTG 
TTGGGGAAGT TCCCTGTGGA CTTCAAAAAC CAGGGGGATG TGGGTGTGGG TGCCATGGCC 
ACGGATGAGA AAGACTTGAT CCTTGTCACA GGAGATTGTA AAGGGCACAT CAAGATTTGG 
GACATCGAGG ATTATTGCAT GTTCACCGAC AAGACACCAC TTCAACCCAG AGGGACAAAC 
AAGTTCCAAT TCTTAATTCC TGAGCAGGTC CAGATCAACC TGCCACACTA CATCCCCTTG 
AAGGAGAAGG AGGTCATGGC TGGCCAGACC ATTTCCCTGG TTCCCCCCAA GCTTCTGAAT 
GCCTGGAAGG GCCATTTGGA TAGTGTGGCA GACATCCTGT ATGTGGATAG CTTCCAGCTG 
ATTATCAGTG CTGGCCACGA TTGTGACGTC AAGGCTTGGA AACTCTCCGG TGACACCATT 
GGGACGTTTG GCGTGAGTGT TTGGAAAGGG TTGCAGGATA TTCAGACGGT TGGTGATCTT 
GCACTAAGAA AAAGCTCAGA GGAGGAGGCT GACTCTATAG ATGCTGCCCA GAAGGCCTTC 
CGTCCAGAGC TGCAGGAGGA GCGGGCTCTT GCCGAGGCCC TGGTGTACCA GCGGCGGGAG 
CAGGTGGCAC TCATGGCTCT CCTGAATGGG AAGGCAGACG CGGAGGCTGA GGCTTGGGGT 
AAGCTGCAGA GGATAACCCA GATGTCCCCA TGGGCTGGAG AGCACTACTC AGAAGACGTT 
GAGCACAGCT GGCTCGAGTG GGAGTCCAAG GGCAAGCAGG TGAGCAAAAT CGTGGGAGGA 
GCATACAAGG CCAAGGACCG GTCTCGGAGT CCCGGAGTCC TGTCCACTAA TGTGCAGTAT 
GGCTGGATGA AGCACCAGAT TTCACCTCAG ATCTACCAAA GCCTGCACTT CAACCAGCTG 
TCACCCACCC ACACACCTGA CTTCCTGGTG CACAATATCC TAGGGCATCA CGGCCAGCAC 
ATCTACCTGG TGACCCACAA TAGCCAAAAG GACCTGGATC CCGAAGAGAC CAACTCGTCT 
ACTGCCTCTG TCTCCCCCAG CGACACCCTC ACCCCGAAAG CCTCAGGCTC CGGGTTCCTG 
GAGTCCGCCT TGTCCACCTC CCCGTACCTC CAGCCCTCCC TCTCCCTCCT GTCCCAGCCA 
TCCAGGTATG TCACGCAGAG GTCGGCGCCC CCCATGCCTG TAGCCTCATC TATCAAACTG 
CTCTCAAGAC CTCTGAAGGC CGCCCTTTCA TCCTCCATGA AGAAGAGCCA CGGCGTCAGG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107227.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962702.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe42875
Clone ID Related Accession (Same CDS sequence)	XM_014107227.1 , XM_014107227.2
Accession Version	XM_014107227.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGGTGGGA GCGGAGGTGA GGCAGAAACA TGTCAGTTCA 
CAAAGCCCCA AGCTGTTAAG AATGCCCCAT GGCAAACAGT CCATCATCAG AGACGCTCAA 
AGGAAAAGAG GGGAATTCCA GAAGAAGATA CTACTGCAAT CCAACACATC GGTGGAGAAG 
GTCATGGCTG GCCAGACCAT TTCCCTGGTT CCCCCCAAGC TTCTGAATGC CTGGAAGGGC 
CATTTGGATA GTGTGGCAGA CATCCTGTAT GTGGATAGCT TCCAGCTGAT TATCAGTGCT 
GGCCACGATT GTGACGTCAA GGCTTGGAAA CTCTCCGGTG ACACCATTGG GACGTTTGGC 
GTGAGTGTTT GGAAAGGGTT GCAGGATATT CAGACGGTTG GTGATCTTGC ACTAAGAAAA 
AGCTCAGAGG AGGAGGCTGA CTCTATAGAT GCTGCCCAGA AGGCCTTCCG TCCAGAGCTG 
CAGGAGGAGC GGGCTCTTGC CGAGGCCCTG GTGTACCAGC GGCGGGAGCA GGTGGCACTC 
ATGGCTCTCC TGAATGGGAA GGCAGACGCG GAGGCTGAGG CTTGGGGTAA GCTGCAGAGG 
ATAACCCAGA TGTCCCCATG GGCTGGAGAG CACTACTCAG AAGACGTTGA GCACAGCTGG 
CTCGAGTGGG AGTCCAAGGG CAAGCAGGTG AGCAAAATCG TGGGAGGAGC ATACAAGGCC 
AAGGACCGGT CTCGGAGTCC CGGAGTCCTG TCCACTAATG TGCAGTATGG CTGGATGAAG 
CACCAGATTT CACCTCAGAT CTACCAAAGC CTGCACTTCA ACCAGCTGTC ACCCACCCAC 
ACACCTGACT TCCTGGTGCA CAATATCCTA GGGCATCACG GCCAGCACAT CTACCTGGTG 
ACCCACAATA GCCAAAAGGA CCTGGATCCC GAAGAGACCA ACTCGTCTAC TGCCTCTGTC 
TCCCCCAGCG ACACCCTCAC CCCGAAAGCC TCAGGCTCCG GGTTCCTGGA GTCCGCCTTG 
TCCACCTCCC CGTACCTCCA GCCCTCCCTC TCCCTCCTGT CCCAGCCATC CAGGTATGTC 
ACGCAGAGGT CGGCGCCCCC CATGCCTGTA GCCTCATCTA TCAAACTGCT CTCAAGACCT 
CTGAAGGCCG CCCTTTCATC CTCCATGAAG AAGAGCCACG GCGTCAGGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962702.1
CDS	1391..4924
Translation	MSQLRWKSKTNIFLSKIASKVTWEEQLGSGSKPSSSEDLRESPQSQEASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGALDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107227.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107227.1

ORF Insert Sequence:

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGGTGGGA GCGGAGGTGA GGCAGAAACA TGTCAGTTCA 
CAAAGCCCCA AGCTGTTAAG AATGCCCCAT GGCAAACAGT CCATCATCAG AGACGCTCAA 
AGGAAAAGAG GGGAATTCCA GAAGAAGATA CTACTGCAAT CCAACACATC GGTGGAGAAG 
GTCATGGCTG GCCAGACCAT TTCCCTGGTT CCCCCCAAGC TTCTGAATGC CTGGAAGGGC 
CATTTGGATA GTGTGGCAGA CATCCTGTAT GTGGATAGCT TCCAGCTGAT TATCAGTGCT 
GGCCACGATT GTGACGTCAA GGCTTGGAAA CTCTCCGGTG ACACCATTGG GACGTTTGGC 
GTGAGTGTTT GGAAAGGGTT GCAGGATATT CAGACGGTTG GTGATCTTGC ACTAAGAAAA 
AGCTCAGAGG AGGAGGCTGA CTCTATAGAT GCTGCCCAGA AGGCCTTCCG TCCAGAGCTG 
CAGGAGGAGC GGGCTCTTGC CGAGGCCCTG GTGTACCAGC GGCGGGAGCA GGTGGCACTC 
ATGGCTCTCC TGAATGGGAA GGCAGACGCG GAGGCTGAGG CTTGGGGTAA GCTGCAGAGG 
ATAACCCAGA TGTCCCCATG GGCTGGAGAG CACTACTCAG AAGACGTTGA GCACAGCTGG 
CTCGAGTGGG AGTCCAAGGG CAAGCAGGTG AGCAAAATCG TGGGAGGAGC ATACAAGGCC 
AAGGACCGGT CTCGGAGTCC CGGAGTCCTG TCCACTAATG TGCAGTATGG CTGGATGAAG 
CACCAGATTT CACCTCAGAT CTACCAAAGC CTGCACTTCA ACCAGCTGTC ACCCACCCAC 
ACACCTGACT TCCTGGTGCA CAATATCCTA GGGCATCACG GCCAGCACAT CTACCTGGTG 
ACCCACAATA GCCAAAAGGA CCTGGATCCC GAAGAGACCA ACTCGTCTAC TGCCTCTGTC 
TCCCCCAGCG ACACCCTCAC CCCGAAAGCC TCAGGCTCCG GGTTCCTGGA GTCCGCCTTG 
TCCACCTCCC CGTACCTCCA GCCCTCCCTC TCCCTCCTGT CCCAGCCATC CAGGTATGTC 
ACGCAGAGGT CGGCGCCCCC CATGCCTGTA GCCTCATCTA TCAAACTGCT CTCAAGACCT 
CTGAAGGCCG CCCTTTCATC CTCCATGAAG AAGAGCCACG GCGTCAGGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107227.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962702.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X9, mRNA.

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CloneID	OCe42875
Clone ID Related Accession (Same CDS sequence)	XM_014107227.1 , XM_014107227.2
Accession Version	XM_014107227.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107227.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGGTGGGA GCGGAGGTGA GGCAGAAACA TGTCAGTTCA 
CAAAGCCCCA AGCTGTTAAG AATGCCCCAT GGCAAACAGT CCATCATCAG AGACGCTCAA 
AGGAAAAGAG GGGAATTCCA GAAGAAGATA CTACTGCAAT CCAACACATC GGTGGAGAAG 
GTCATGGCTG GCCAGACCAT TTCCCTGGTT CCCCCCAAGC TTCTGAATGC CTGGAAGGGC 
CATTTGGATA GTGTGGCAGA CATCCTGTAT GTGGATAGCT TCCAGCTGAT TATCAGTGCT 
GGCCACGATT GTGACGTCAA GGCTTGGAAA CTCTCCGGTG ACACCATTGG GACGTTTGGC 
GTGAGTGTTT GGAAAGGGTT GCAGGATATT CAGACGGTTG GTGATCTTGC ACTAAGAAAA 
AGCTCAGAGG AGGAGGCTGA CTCTATAGAT GCTGCCCAGA AGGCCTTCCG TCCAGAGCTG 
CAGGAGGAGC GGGCTCTTGC CGAGGCCCTG GTGTACCAGC GGCGGGAGCA GGTGGCACTC 
ATGGCTCTCC TGAATGGGAA GGCAGACGCG GAGGCTGAGG CTTGGGGTAA GCTGCAGAGG 
ATAACCCAGA TGTCCCCATG GGCTGGAGAG CACTACTCAG AAGACGTTGA GCACAGCTGG 
CTCGAGTGGG AGTCCAAGGG CAAGCAGGTG AGCAAAATCG TGGGAGGAGC ATACAAGGCC 
AAGGACCGGT CTCGGAGTCC CGGAGTCCTG TCCACTAATG TGCAGTATGG CTGGATGAAG 
CACCAGATTT CACCTCAGAT CTACCAAAGC CTGCACTTCA ACCAGCTGTC ACCCACCCAC 
ACACCTGACT TCCTGGTGCA CAATATCCTA GGGCATCACG GCCAGCACAT CTACCTGGTG 
ACCCACAATA GCCAAAAGGA CCTGGATCCC GAAGAGACCA ACTCGTCTAC TGCCTCTGTC 
TCCCCCAGCG ACACCCTCAC CCCGAAAGCC TCAGGCTCCG GGTTCCTGGA GTCCGCCTTG 
TCCACCTCCC CGTACCTCCA GCCCTCCCTC TCCCTCCTGT CCCAGCCATC CAGGTATGTC 
ACGCAGAGGT CGGCGCCCCC CATGCCTGTA GCCTCATCTA TCAAACTGCT CTCAAGACCT 
CTGAAGGCCG CCCTTTCATC CTCCATGAAG AAGAGCCACG GCGTCAGGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962702.1
CDS	1140..4673
Translation	MSQLRWKSKTNIFLSKIASKVTWEEQLGSGSKPSSSEDLRESPQSQEASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGALDMKTFIKAIKKILSNASDEMLEALFLKVDTDCNGSITWQEYVDYVMRELQRRERMRTSQYRLHFHLPMRIIPLNHGCEIVKVQFLTQRFKKMGHLLTVTKDGILQIWSESFSLLNSFRLNQIHQPYNQQMWVTDMVCLHNMNLIAIASTDQKIEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107227.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107227.2

ORF Insert Sequence:

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGCCCTG 
GACATGAAGA CTTTCATTAA GGCCATAAAG AAGATTCTGA GCAATGCGTC AGATGAGATG 
CTAGAGGCGC TGTTTTTGAA GGTGGACACA GACTGTAATG GCTCCATTAC CTGGCAGGAG 
TACGTGGATT ACGTGATGCG GGAGTTACAG AGAAGGGAGA GGATGAGAAC AAGCCAGTAC 
CGCCTGCACT TCCACCTTCC CATGAGGATC ATTCCCCTGA ACCATGGATG CGAGATTGTG 
AAGGTGCAGT TCTTAACTCA GCGGTTCAAG AAGATGGGGC ACCTTCTGAC TGTCACCAAG 
GACGGGATCT TGCAGATCTG GTCTGAATCC TTCTCGCTGC TTAACTCCTT TAGGCTTAAC 
CAGATCCATC AGCCCTATAA CCAGCAGATG TGGGTCACTG ATATGGTGTG TCTCCATAAC 
ATGAACCTGA TTGCGATTGC ATCAACTGAC CAGAAAATAG AGTTCTTTGA TATTACCAAC 
CATAATTGTG CCCGGGCCTT CACCTTCATT GATCTGGACA GCTGTGTCCT GACCATGGAC 
TATTGGTCCG ACTATCACAG AGGTGTATTC TGCTACGGGG ACACCAAAGG CAATGTCATT 
GTCTTCACCT CTGACAATGT GACCCACGGG CTGTTCAACC CTCGAATCCT TCCCAGGACC 
TCCAAATGGG ATCACTGGAC CAAAGTTTCT ACACGGAAGC TTCTAAATGA GAAGTCTCCT 
TTGTACAGGA GTTATTGGCT AAAGGCTCTC CATCCCAACT GGTGCCAACA GGTCAAGTTC 
ATTCCTCAGC TGAACACAGT GGCCTCCTGT TCGGCCATTG AGAAGTCCTC TCTGGTGCTA 
ATAGCATTGC CATCTAAAGC CCCAGAGAGC CCCAAGTTTT CAGTGCTGAA CTTAAGAAAA 
GGGATTCTTT GCTTCGATTA CTGCCCAGAC AAGAACTTAG TGGTGACTGG TGGCTATGAC 
CCCCTCATCC GCCTGTGGAA CCCCTTCTTC TCAAAAAAGC CTGTGTGGAT GATGAAGGGG 
CATCAGACTT CCGTGACGCA CATCCTCGTG AACAGCAAGA ACAGCAGCAT CCTCCTCAGC 
ATCTCCAAGG ACAAGAATAT CCGTGTGTGG GACATGCAGG ACTACATATG CCTCCAGTCC 
TTTTCCGGGA AGCTTTTTGC CCTGGGAAAC TGCCCCATCA CCAGTGTCTA CTTCCACAAG 
AATGAAGACA GTCTCATCTG TAGCACCTTT TCAATTGGGA TCCTAAAAGG GTACTTGGAG 
ACTGAGGGGC CTGGGAAACC AAGGGAGAGG ACCACAACTT ATAGCACACC CCTGTGCGCT 
GTTCTCTACA GCAAGATTTT TAAGCAGGTG GTAAGCGGCT GCCTGAGCAG CTTGGTGAGT 
GTGTGGGAGA TCAAGACAGG CAAGAGGATG ATGGAGTTCT CTGTGACTGG CGACCAGCAA 
GTGGAGCTGA CCACCATGTG CCTGGATGAA TCCGAGCGGT GCCTGCTCAC GGGTTTGCGC 
GACGGCACCA TAAAGATGTG GAACTACACT GTGGGTGAAT GCCTGCTGAC CTTCCCCAAC 
CCGGACCAGC TGGAGATTAG CGGGATTGTC CATATGAACA AAGTGTTCTA CGTGACTGGA 
TGGAGTAAGA GAATCACCTG TTTCATGCTC CATAAGACCA AGCCAGTCCT CCTGTGCTAC 
CAATGGCAGA GCTTCCACAA GGAAGATGTT CTGAGCATGG CCAAGTACCA AAACCAGTTC 
CTTGGGACCT CTTCCTACAA TGGAGATATT CTCTTCTGGA ACATCAACCT GTTCAAGCCC 
ATCCTCAATT TCAATGCTTC TAAGAGCCCC TGGCCTTTGC TGCCCAAGAA GATCCAAGAA 
GTGGACAACT CCCTGGCTGA GAGCCTCCAG TCCAGCAAGC TTTGTGTGAA ACATAAGGAG 
TGGGCGTACA AACCATCAAC GCAGCCCCTG GGCCCCAGAG CCAGGGCTGT AGCCAATGCC 
AACCTGCAAC GGAACCTGAT GTCAGCTCCC CCAGTGATGA AGCGCCCCAG AGACAAGGAG 
CCAGAAAAGC CCATGCCCCC GCAGGTGGGA GCGGAGGTGA GGCAGAAACA TGTCAGTTCA 
CAAAGCCCCA AGCTGTTAAG AATGCCCCAT GGCAAACAGT CCATCATCAG AGACGCTCAA 
AGGAAAAGAG GGGAATTCCA GAAGAAGATA CTACTGCAAT CCAACACATC GGTGGAGAAG 
GTCATGGCTG GCCAGACCAT TTCCCTGGTT CCCCCCAAGC TTCTGAATGC CTGGAAGGGC 
CATTTGGATA GTGTGGCAGA CATCCTGTAT GTGGATAGCT TCCAGCTGAT TATCAGTGCT 
GGCCACGATT GTGACGTCAA GGCTTGGAAA CTCTCCGGTG ACACCATTGG GACGTTTGGC 
GTGAGTGTTT GGAAAGGGTT GCAGGATATT CAGACGGTTG GTGATCTTGC ACTAAGAAAA 
AGCTCAGAGG AGGAGGCTGA CTCTATAGAT GCTGCCCAGA AGGCCTTCCG TCCAGAGCTG 
CAGGAGGAGC GGGCTCTTGC CGAGGCCCTG GTGTACCAGC GGCGGGAGCA GGTGGCACTC 
ATGGCTCTCC TGAATGGGAA GGCAGACGCG GAGGCTGAGG CTTGGGGTAA GCTGCAGAGG 
ATAACCCAGA TGTCCCCATG GGCTGGAGAG CACTACTCAG AAGACGTTGA GCACAGCTGG 
CTCGAGTGGG AGTCCAAGGG CAAGCAGGTG AGCAAAATCG TGGGAGGAGC ATACAAGGCC 
AAGGACCGGT CTCGGAGTCC CGGAGTCCTG TCCACTAATG TGCAGTATGG CTGGATGAAG 
CACCAGATTT CACCTCAGAT CTACCAAAGC CTGCACTTCA ACCAGCTGTC ACCCACCCAC 
ACACCTGACT TCCTGGTGCA CAATATCCTA GGGCATCACG GCCAGCACAT CTACCTGGTG 
ACCCACAATA GCCAAAAGGA CCTGGATCCC GAAGAGACCA ACTCGTCTAC TGCCTCTGTC 
TCCCCCAGCG ACACCCTCAC CCCGAAAGCC TCAGGCTCCG GGTTCCTGGA GTCCGCCTTG 
TCCACCTCCC CGTACCTCCA GCCCTCCCTC TCCCTCCTGT CCCAGCCATC CAGGTATGTC 
ACGCAGAGGT CGGCGCCCCC CATGCCTGTA GCCTCATCTA TCAAACTGCT CTCAAGACCT 
CTGAAGGCCG CCCTTTCATC CTCCATGAAG AAGAGCCACG GCGTCAGGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB8 ( XM_014107228.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013962703.1 Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X9, mRNA.

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CloneID	OCe42876
Clone ID Related Accession (Same CDS sequence)	XM_014107228.1
Accession Version	XM_014107228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	EF-hand calcium-binding domain-containing protein 8 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGAGTTC 
TTTGATATTA CCAACCATAA TTGTGCCCGG GCCTTCACCT TCATTGATCT GGACAGCTGT 
GTCCTGACCA TGGACTATTG GTCCGACTAT CACAGAGGTG TATTCTGCTA CGGGGACACC 
AAAGGCAATG TCATTGTCTT CACCTCTGAC AATGTGACCC ACGGGCTGTT CAACCCTCGA 
ATCCTTCCCA GGACCTCCAA ATGGGATCAC TGGACCAAAG TTTCTACACG GAAGCTTCTA 
AATGAGAAGT CTCCTTTGTA CAGGAGTTAT TGGCTAAAGG CTCTCCATCC CAACTGGTGC 
CAACAGGTCA AGTTCATTCC TCAGCTGAAC ACAGTGGCCT CCTGTTCGGC CATTGAGAAG 
TCCTCTCTGG TGCTAATAGC ATTGCCATCT AAAGCCCCAG AGAGCCCCAA GTTTTCAGTG 
CTGAACTTAA GAAAAGGGAT TCTTTGCTTC GATTACTGCC CAGACAAGAA CTTAGTGGTG 
ACTGGTGGCT ATGACCCCCT CATCCGCCTG TGGAACCCCT TCTTCTCAAA AAAGCCTGTG 
TGGATGATGA AGGGGCATCA GACTTCCGTG ACGCACATCC TCGTGAACAG CAAGAACAGC 
AGCATCCTCC TCAGCATCTC CAAGGACAAG AATATCCGTG TGTGGGACAT GCAGGACTAC 
ATATGCCTCC AGTCCTTTTC CGGGAAGCTT TTTGCCCTGG GAAACTGCCC CATCACCAGT 
GTCTACTTCC ACAAGAATGA AGACAGTCTC ATCTGTAGCA CCTTTTCAAT TGGGATCCTA 
AAAGGGTACT TGGAGACTGA GGGGCCTGGG AAACCAAGGG AGAGGACCAC AACTTATAGC 
ACACCCCTGT GCGCTGTTCT CTACAGCAAG ATTTTTAAGC AGGTGGTAAG CGGCTGCCTG 
AGCAGCTTGG TGAGTGTGTG GGAGATCAAG ACAGGCAAGA GGATGATGGA GTTCTCTGTG 
ACTGGCGACC AGCAAGTGGA GCTGACCACC ATGTGCCTGG ATGAATCCGA GCGGTGCCTG 
CTCACGGGTT TGCGCGACGG CACCATAAAG ATGTGGAACT ACACTGTGGG TGAATGCCTG 
CTGACCTTCC CCAACCCGGA CCAGCTGGAG ATTAGCGGGA TTGTCCATAT GAACAAAGTG 
TTCTACGTGA CTGGATGGAG TAAGAGAATC ACCTGTTTCA TGCTCCATAA GACCAAGCCA 
GTCCTCCTGT GCTACCAATG GCAGAGCTTC CACAAGGAAG ATGTTCTGAG CATGGCCAAG 
TACCAAAACC AGTTCCTTGG GACCTCTTCC TACAATGGAG ATATTCTCTT CTGGAACATC 
AACCTGTTCA AGCCCATCCT CAATTTCAAT GCTTCTAAGA GCCCCTGGCC TTTGCTGCCC 
AAGAAGATCC AAGAAGTGGA CAACTCCCTG GCTGAGAGCC TCCAGTCCAG CAAGCTTTGT 
GTGAAACATA AGGAGTGGGC GTACAAACCA TCAACGCAGC CCCTGGGCCC CAGAGCCAGG 
GCTGTAGCCA ATGCCAACCT GCAACGGAAC CTGATGTCAG CTCCCCCAGT GATGAAGCGC 
CCCAGAGACA AGGAGCCAGA AAAGCCCATG CCCCCGCAGG TGGGAGCGGA GGTGAGGCAG 
AAACATGTCA GTTCACAAAG CCCCAAGCTG TTAAGAATGC CCCATGGCAA ACAGTCCATC 
ATCAGAGACG CTCAAAGGAA AAGAGGGGAA TTCCAGAAGA AGATACTACT GCAATCCAAC 
ACATCGGTGG AGAAGATAAT CTTCCTGCAG ACCAGGCCTC GCCTGCCACA CATAGCTGCC 
CTGCTGAGCA GCTGCATCGA TGGCTACATC TATGCCTGGT CCATCCACGG GAGCGGAGGC 
CTGTTGGGGA AGTTCCCTGT GGACTTCAAA AACCAGGGGG ATGTGGGTGT GGGTGCCATG 
GCCACGGATG AGAAAGACTT GATCCTTGTC ACAGGAGATT GTAAAGGGCA CATCAAGATT 
TGGGACATCG AGGATTATTG CATGTTCACC GACAAGACAC CACTTCAACC CAGAGGGACA 
AACAAGTTCC AATTCTTAAT TCCTGAGCAG GTCCAGATCA ACCTGCCACA CTACATCCCC 
TTGAAGGAGA AGGAGGTCAT GGCTGGCCAG ACCATTTCCC TGGTTCCCCC CAAGCTTCTG 
AATGCCTGGA AGGGCCATTT GGATAGTGTG GCAGACATCC TGTATGTGGA TAGCTTCCAG 
CTGATTATCA GTGCTGGCCA CGATTGTGAC GTCAAGGCTT GGAAACTCTC CGGTGACACC 
ATTGGGACGT TTGGCGTGAG TGTTTGGAAA GGGTTGCAGG ATATTCAGAC GGTTGGTGAT 
CTTGCACTAA GAAAAAGCTC AGAGGAGGAG GCTGACTCTA TAGATGCTGC CCAGAAGGCC 
TTCCGTCCAG AGCTGCAGGA GGAGCGGGCT CTTGCCGAGG CCCTGGTGTA CCAGCGGCGG 
GAGCAGGTGG CACTCATGGC TCTCCTGAAT GGGAAGGCAG ACGCGGAGGC TGAGGCTTGG 
GGTAAGCTGC AGAGGATAAC CCAGATGTCC CCATGGGCTG GAGAGCACTA CTCAGAAGAC 
GTTGAGCACA GCTGGCTCGA GTGGGAGTCC AAGGGCAAGC AGGTGAGCAA AATCGTGGGA 
GGAGCATACA AGGCCAAGGA CCGGTCTCGG AGTCCCGGAG TCCTGTCCAC TAATGTGCAG 
TATGGCTGGA TGAAGCACCA GATTTCACCT CAGATCTACC AAAGCCTGCA CTTCAACCAG 
CTGTCACCCA CCCACACACC TGACTTCCTG GTGCACAATA TCCTAGGGCA TCACGGCCAG 
CACATCTACC TGGTGACCCA CAATAGCCAA AAGGACCTGG ATCCCGAAGA GACCAACTCG 
TCTACTGCCT CTGTCTCCCC CAGCGACACC CTCACCCCGA AAGCCTCAGG CTCCGGGTTC 
CTGGAGTCCG CCTTGTCCAC CTCCCCGTAC CTCCAGCCCT CCCTCTCCCT CCTGTCCCAG 
CCATCCAGGT ATGTCACGCA GAGGTCGGCG CCCCCCATGC CTGTAGCCTC ATCTATCAAA 
CTGCTCTCAA GACCTCTGAA GGCCGCCCTT TCATCCTCCA TGAAGAAGAG CCACGGCGTC 
AGGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962703.1
CDS	1391..4819
Translation	MSQLRWKSKTNIFLSKIASKVTWEEQLGSGSKPSSSEDLRESPQSQEASTPGGFQKNEEVLSSPASTVTLSHHESQLFTQLHMVKMEKMFEDDVDSTGEFFDITNHNCARAFTFIDLDSCVLTMDYWSDYHRGVFCYGDTKGNVIVFTSDNVTHGLFNPRILPRTSKWDHWTKVSTRKLLNEKSPLYRSYWLKALHPNWCQQVKFIPQLNTVASCSAIEKSSLVLIALPSKAPESPKFSVLNLRKGILCFDYCPDKNLVVTGGYDPLIRLWNPFFSKKPVWMMKGHQTSVTHILVNSKNSSILLSISKDKNIRVWDMQDYICLQSFSGKLFALGNCPITSVYFHKNEDSLICSTFSIGILKGYLETEGPGKPRERTTTYSTPLCAVLYSKIFKQVVSGCLSSLVSVWEIKTGKRMMEFSVTGDQQVELTTMCLDESERCLLTGLRDGTIKMWNYTVGECLLTFPNPDQLEISGIVHMNKVFYVTGWSKRITCFMLHKTKPVLLCYQWQSFHKEDVLSMAKYQNQFLGTSSYNGDILFWNINLFKPILNFNASKSPWPLLPKKIQEVDNSLAESLQSSKLCVKHKEWAYKPSTQPLGPRARAVANANLQRNLMSAPPVMKRPRDKEPEKPMPPQVGAEVRQKHVSSQSPKLLRMPHGKQSIIRDAQRKRGEFQKKILLQSNTSVEKIIFLQTRPRLPHIAALLSSCIDGYIYAWSIHGSGGLLGKFPVDFKNQGDVGVGAMATDEKDLILVTGDCKGHIKIWDIEDYCMFTDKTPLQPRGTNKFQFLIPEQVQINLPHYIPLKEKEVMAGQTISLVPPKLLNAWKGHLDSVADILYVDSFQLIISAGHDCDVKAWKLSGDTIGTFGVSVWKGLQDIQTVGDLALRKSSEEEADSIDAAQKAFRPELQEERALAEALVYQRREQVALMALLNGKADAEAEAWGKLQRITQMSPWAGEHYSEDVEHSWLEWESKGKQVSKIVGGAYKAKDRSRSPGVLSTNVQYGWMKHQISPQIYQSLHFNQLSPTHTPDFLVHNILGHHGQHIYLVTHNSQKDLDPEETNSSTASVSPSDTLTPKASGSGFLESALSTSPYLQPSLSLLSQPSRYVTQRSAPPMPVASSIKLLSRPLKAALSSSMKKSHGVRF

Target ORF information:

RefSeq Version	XM_014107228.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris EF-hand calcium binding domain 8 (EFCAB8), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107228.1

ORF Insert Sequence:

ATGTCTCAGT TGAGATGGAA GTCGAAAACC AATATATTTC TGTCAAAAAT AGCCAGCAAG 
GTTACTTGGG AAGAACAACT TGGGTCAGGG TCAAAACCAT CATCTTCTGA GGACTTAAGA 
GAGAGCCCTC AGTCTCAAGA GGCATCCACC CCAGGTGGAT TCCAGAAGAA TGAGGAGGTT 
CTTAGCTCCC CAGCATCAAC TGTCACTCTT AGCCATCATG AGTCTCAACT GTTTACCCAG 
CTACACATGG TCAAGATGGA GAAAATGTTT GAGGATGATG TTGACTCGAC TGGAGAGTTC 
TTTGATATTA CCAACCATAA TTGTGCCCGG GCCTTCACCT TCATTGATCT GGACAGCTGT 
GTCCTGACCA TGGACTATTG GTCCGACTAT CACAGAGGTG TATTCTGCTA CGGGGACACC 
AAAGGCAATG TCATTGTCTT CACCTCTGAC AATGTGACCC ACGGGCTGTT CAACCCTCGA 
ATCCTTCCCA GGACCTCCAA ATGGGATCAC TGGACCAAAG TTTCTACACG GAAGCTTCTA 
AATGAGAAGT CTCCTTTGTA CAGGAGTTAT TGGCTAAAGG CTCTCCATCC CAACTGGTGC 
CAACAGGTCA AGTTCATTCC TCAGCTGAAC ACAGTGGCCT CCTGTTCGGC CATTGAGAAG 
TCCTCTCTGG TGCTAATAGC ATTGCCATCT AAAGCCCCAG AGAGCCCCAA GTTTTCAGTG 
CTGAACTTAA GAAAAGGGAT TCTTTGCTTC GATTACTGCC CAGACAAGAA CTTAGTGGTG 
ACTGGTGGCT ATGACCCCCT CATCCGCCTG TGGAACCCCT TCTTCTCAAA AAAGCCTGTG 
TGGATGATGA AGGGGCATCA GACTTCCGTG ACGCACATCC TCGTGAACAG CAAGAACAGC 
AGCATCCTCC TCAGCATCTC CAAGGACAAG AATATCCGTG TGTGGGACAT GCAGGACTAC 
ATATGCCTCC AGTCCTTTTC CGGGAAGCTT TTTGCCCTGG GAAACTGCCC CATCACCAGT 
GTCTACTTCC ACAAGAATGA AGACAGTCTC ATCTGTAGCA CCTTTTCAAT TGGGATCCTA 
AAAGGGTACT TGGAGACTGA GGGGCCTGGG AAACCAAGGG AGAGGACCAC AACTTATAGC 
ACACCCCTGT GCGCTGTTCT CTACAGCAAG ATTTTTAAGC AGGTGGTAAG CGGCTGCCTG 
AGCAGCTTGG TGAGTGTGTG GGAGATCAAG ACAGGCAAGA GGATGATGGA GTTCTCTGTG 
ACTGGCGACC AGCAAGTGGA GCTGACCACC ATGTGCCTGG ATGAATCCGA GCGGTGCCTG 
CTCACGGGTT TGCGCGACGG CACCATAAAG ATGTGGAACT ACACTGTGGG TGAATGCCTG 
CTGACCTTCC CCAACCCGGA CCAGCTGGAG ATTAGCGGGA TTGTCCATAT GAACAAAGTG 
TTCTACGTGA CTGGATGGAG TAAGAGAATC ACCTGTTTCA TGCTCCATAA GACCAAGCCA 
GTCCTCCTGT GCTACCAATG GCAGAGCTTC CACAAGGAAG ATGTTCTGAG CATGGCCAAG 
TACCAAAACC AGTTCCTTGG GACCTCTTCC TACAATGGAG ATATTCTCTT CTGGAACATC 
AACCTGTTCA AGCCCATCCT CAATTTCAAT GCTTCTAAGA GCCCCTGGCC TTTGCTGCCC 
AAGAAGATCC AAGAAGTGGA CAACTCCCTG GCTGAGAGCC TCCAGTCCAG CAAGCTTTGT 
GTGAAACATA AGGAGTGGGC GTACAAACCA TCAACGCAGC CCCTGGGCCC CAGAGCCAGG 
GCTGTAGCCA ATGCCAACCT GCAACGGAAC CTGATGTCAG CTCCCCCAGT GATGAAGCGC 
CCCAGAGACA AGGAGCCAGA AAAGCCCATG CCCCCGCAGG TGGGAGCGGA GGTGAGGCAG 
AAACATGTCA GTTCACAAAG CCCCAAGCTG TTAAGAATGC CCCATGGCAA ACAGTCCATC 
ATCAGAGACG CTCAAAGGAA AAGAGGGGAA TTCCAGAAGA AGATACTACT GCAATCCAAC 
ACATCGGTGG AGAAGATAAT CTTCCTGCAG ACCAGGCCTC GCCTGCCACA CATAGCTGCC 
CTGCTGAGCA GCTGCATCGA TGGCTACATC TATGCCTGGT CCATCCACGG GAGCGGAGGC 
CTGTTGGGGA AGTTCCCTGT GGACTTCAAA AACCAGGGGG ATGTGGGTGT GGGTGCCATG 
GCCACGGATG AGAAAGACTT GATCCTTGTC ACAGGAGATT GTAAAGGGCA CATCAAGATT 
TGGGACATCG AGGATTATTG CATGTTCACC GACAAGACAC CACTTCAACC CAGAGGGACA 
AACAAGTTCC AATTCTTAAT TCCTGAGCAG GTCCAGATCA ACCTGCCACA CTACATCCCC 
TTGAAGGAGA AGGAGGTCAT GGCTGGCCAG ACCATTTCCC TGGTTCCCCC CAAGCTTCTG 
AATGCCTGGA AGGGCCATTT GGATAGTGTG GCAGACATCC TGTATGTGGA TAGCTTCCAG 
CTGATTATCA GTGCTGGCCA CGATTGTGAC GTCAAGGCTT GGAAACTCTC CGGTGACACC 
ATTGGGACGT TTGGCGTGAG TGTTTGGAAA GGGTTGCAGG ATATTCAGAC GGTTGGTGAT 
CTTGCACTAA GAAAAAGCTC AGAGGAGGAG GCTGACTCTA TAGATGCTGC CCAGAAGGCC 
TTCCGTCCAG AGCTGCAGGA GGAGCGGGCT CTTGCCGAGG CCCTGGTGTA CCAGCGGCGG 
GAGCAGGTGG CACTCATGGC TCTCCTGAAT GGGAAGGCAG ACGCGGAGGC TGAGGCTTGG 
GGTAAGCTGC AGAGGATAAC CCAGATGTCC CCATGGGCTG GAGAGCACTA CTCAGAAGAC 
GTTGAGCACA GCTGGCTCGA GTGGGAGTCC AAGGGCAAGC AGGTGAGCAA AATCGTGGGA 
GGAGCATACA AGGCCAAGGA CCGGTCTCGG AGTCCCGGAG TCCTGTCCAC TAATGTGCAG 
TATGGCTGGA TGAAGCACCA GATTTCACCT CAGATCTACC AAAGCCTGCA CTTCAACCAG 
CTGTCACCCA CCCACACACC TGACTTCCTG GTGCACAATA TCCTAGGGCA TCACGGCCAG 
CACATCTACC TGGTGACCCA CAATAGCCAA AAGGACCTGG ATCCCGAAGA GACCAACTCG 
TCTACTGCCT CTGTCTCCCC CAGCGACACC CTCACCCCGA AAGCCTCAGG CTCCGGGTTC 
CTGGAGTCCG CCTTGTCCAC CTCCCCGTAC CTCCAGCCCT CCCTCTCCCT CCTGTCCCAG 
CCATCCAGGT ATGTCACGCA GAGGTCGGCG CCCCCCATGC CTGTAGCCTC ATCTATCAAA 
CTGCTCTCAA GACCTCTGAA GGCCGCCCTT TCATCCTCCA TGAAGAAGAG CCACGGCGTC 
AGGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	EFCAB8
Entrez Gene ID	102156949
Full Name	EF-hand calcium binding domain 8
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

EFCAB8 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

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