ARHGEF1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGEF1
Entrez Gene ID	100856354
Full Name	Rho guanine nucleotide exchange factor 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014120090.2	XP_013975565.1	rho guanine nucleotide exchange factor 1 isoform X4
XM_014120090.1	XP_013975565.1	rho guanine nucleotide exchange factor 1 isoform X4
XM_014120086.2	XP_013975561.1	rho guanine nucleotide exchange factor 1 isoform X3
XM_014120077.1	XP_013975552.1	rho guanine nucleotide exchange factor 1 isoform X1
XM_014120083.1	XP_013975558.1	rho guanine nucleotide exchange factor 1 isoform X2
XM_014120077.2	XP_013975552.1	rho guanine nucleotide exchange factor 1 isoform X1
XM_014120083.2	XP_013975558.1	rho guanine nucleotide exchange factor 1 isoform X2
XM_014120086.1	XP_013975561.1	rho guanine nucleotide exchange factor 1 isoform X3

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	arhgef1	XP_002941974.2
Canis lupus familiaris (dog)	ARHGEF1	XP_005616572.1
Canis lupus familiaris (dog)	LOC100856005	XP_003640357.2
Pan troglodytes (chimpanzee)	ARHGEF1	XP_003953551.1
Mus musculus (house mouse)	Arhgef1	NP_032514.1
Rattus norvegicus (Norway rat)	Arhgef1	NP_067726.3
Homo sapiens (human)	ARHGEF1	NP_945353.1
Bos taurus (cattle)	ARHGEF1	XP_005219451.1

Gene Ontology

The following ARHGEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe02096	XM_014120077.2 Latest version!	Canis lupus familiaris Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe02097	XM_014120083.2 Latest version!	Canis lupus familiaris Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe02098	XM_014120086.2 Latest version!	Canis lupus familiaris Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe35012	XM_014120090.2 Latest version!	Canis lupus familiaris Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OCe35012	XM_014120090.1	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe02096	XM_014120077.1	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe02097	XM_014120083.1	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OCe02098	XM_014120086.1	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGEF1 ( XM_014120077.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013975552.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe02096
Clone ID Related Accession (Same CDS sequence)	XM_014120077.1 , XM_014120077.2
Accession Version	XM_014120077.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rho guanine nucleotide exchange factor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGAAGGTG GAAGAGGCTG 
TCGGGGCGTC TGGGGCGCTC GGAGAGCCTG CGGGTGAGTG ACCGCCGCCG GCCTTCCCGG 
GGCAGCCTCG GGGCTAAGGG CCGGGGTGGG GGCCGCTCCC GGAGTGACGT GGACATGGAC 
CCCAGCTCTG CCACGGCCGT GCTTGGCCCT GCCCGACGAG CCACCCCTGA GCCTGGAGAC 
GAAGGGGACC CTGGGCGGTC AGGACTAGAG CTGGAACCAG AAGAGCCCCC TGGCTGGCGG 
GAGCTCATCC CCCCAGGCAC CCTGCACAGC CTGCCTAAGA GCCAGGTGAA GCGGCAGGAG 
GTCATCAGCG AGCTACTGGT GACAGAGGCT GCCCATGTGC GCATGCTCCG GGTGCTGCAT 
GACCTCTTCT ACCAGCCCAT GTTGGAAGGG AACTTCTTCT CCACGGAGGA TCTACAGAAC 
ATCTTCCCCA GCCTGGATGA GCTCATCGAG GTGCATTCCC TGTTCCTCGA TCGACTGATG 
AAGCGGAGGC AGGATAGTGG CTGCCTCATT GAGGAGATCG GAGATGTACT GCTGGCCCGG 
TTTGATGGTG CCGAAGGCAA ATGGTTCCAG AAAATCTCCT CCCGCTTCTG CAGCCGCCAG 
TCATTTGCCT TAGAGCAGCT CAAAGCCAAG CAGCGCAAGG ATCCTCGGTT CTGTGCCTTT 
GTGCAGGAGG CCGAGAGCCG CCCCCGGTGC CGCCGCCTGC AGCTGAAGGA TATGATCCCC 
ACGGAGATGC AGCGTCTGAC CAAGTACCCC CTGCTCCTGC AGAGCATTGG GCAGAACACA 
GAAGAGTCAG CAGAACGGGA GAAAGTAGAA CTGGCAGCTG AGTGCTGCCG GGAAATTCTG 
CACCATGTTA ACCAAGCCGT GCGTGACATG GAGGACCTGC TGCGGCTCAA GGATTATCAG 
AAGCGCCTGG ACTTGTCCCT CCTGCGGCAG AGCAGCGACC CCATGCTAAG CGAGTTCAAG 
AACCTGGACA TCACCAAAAA GAAGCTGATC TACGAGGGTC CGCTGACGTG GCGGCTGACG 
AAGGACAAGG CTGTAGAGGT ACACGTGCTG CTGCTGGATG ACCTGCTGCT GCTGCTCCAG 
CGCCAGGACG ATCGGCTGCT GCTCAAGTCC CACAGCCGGA CGCTGACACC CACACCCGAC 
GGCAAGACCA TGCTGCGGCC GGTGCTGCGG CTCACCTCCG CCATAACCCG CGAGGTGGCT 
ACCGATCACA AAGCCTTCTA TGTCCTTTTT ACCTGGGACC AGGAGGCCCA GATATATGAG 
CTGGTGGCCC AGACGGTGTC GGAACGGAAG AACTGGTGTT CCCTCATCAC TGAAACTGCT 
GGATCCTTGA AGGTCCCTGC CCCCGCCTCT CGCCCCAAAC CCCGGCCCAG CCCAAGCAGC 
ACCCGAGAAC CCCTGCTCAG CAGCTCAGAG AATGGCAACG GTGGTGGCCG AGAGATGCCC 
CCAGCTGACG GCCAGACGGA GAGAATCCTC AGCACCCTCC TGCCCTACTG CAGGCCCCGC 
CCTGAGGGCC AGCTTGCTGC CAAGGCCCTT GAGAAAGTGC TGTCCCTGAA GCAGCTCCTG 
TTTCCCTCGG AGGAAGACAG TGGGGCAGGG CCTCCCCAGG AAGGGGATGG GGTCCCTGGG 
GGTGGCCCCC TGAACCCGAC ACAGACCCAC ACCCAGGAAA TTCGGGAAAA GCTGCTCAGC 
CTGGAGGAGA CCATGAAACA GCTGGAGGAG GTGGAGGAGG AATTTTGTCA CCTGCGACTC 
CTCCTGTCTC AGCTTGGGGA GAACAGCACC CCCCAGCCTG GGTGTACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975552.1
CDS	160..3090
Translation	MEADNVARGLAPGPPRPGLVPVSIIGAEDEDFENELETNTEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTERRWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPARRATPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQKRLDLSLLRQSSDPMLSEFKNLDITKKKLIYEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDDRLLLKSHSRTLTPTPDGKTMLRPVLRLTSAITREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCSLITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGGREMPPADGQTERILSTLLPYCRPRPEGQLAAKALEKVLSLKQLLFPSEEDSGAGPPQEGDGVPGGGPLNPTQTHTQEIREKLLSLEETMKQLEEVEEEFCHLRLLLSQLGENSTPQPGCT

Target ORF information:

RefSeq Version	XM_014120077.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120077.1

ORF Insert Sequence:

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGAAGGTG GAAGAGGCTG 
TCGGGGCGTC TGGGGCGCTC GGAGAGCCTG CGGGTGAGTG ACCGCCGCCG GCCTTCCCGG 
GGCAGCCTCG GGGCTAAGGG CCGGGGTGGG GGCCGCTCCC GGAGTGACGT GGACATGGAC 
CCCAGCTCTG CCACGGCCGT GCTTGGCCCT GCCCGACGAG CCACCCCTGA GCCTGGAGAC 
GAAGGGGACC CTGGGCGGTC AGGACTAGAG CTGGAACCAG AAGAGCCCCC TGGCTGGCGG 
GAGCTCATCC CCCCAGGCAC CCTGCACAGC CTGCCTAAGA GCCAGGTGAA GCGGCAGGAG 
GTCATCAGCG AGCTACTGGT GACAGAGGCT GCCCATGTGC GCATGCTCCG GGTGCTGCAT 
GACCTCTTCT ACCAGCCCAT GTTGGAAGGG AACTTCTTCT CCACGGAGGA TCTACAGAAC 
ATCTTCCCCA GCCTGGATGA GCTCATCGAG GTGCATTCCC TGTTCCTCGA TCGACTGATG 
AAGCGGAGGC AGGATAGTGG CTGCCTCATT GAGGAGATCG GAGATGTACT GCTGGCCCGG 
TTTGATGGTG CCGAAGGCAA ATGGTTCCAG AAAATCTCCT CCCGCTTCTG CAGCCGCCAG 
TCATTTGCCT TAGAGCAGCT CAAAGCCAAG CAGCGCAAGG ATCCTCGGTT CTGTGCCTTT 
GTGCAGGAGG CCGAGAGCCG CCCCCGGTGC CGCCGCCTGC AGCTGAAGGA TATGATCCCC 
ACGGAGATGC AGCGTCTGAC CAAGTACCCC CTGCTCCTGC AGAGCATTGG GCAGAACACA 
GAAGAGTCAG CAGAACGGGA GAAAGTAGAA CTGGCAGCTG AGTGCTGCCG GGAAATTCTG 
CACCATGTTA ACCAAGCCGT GCGTGACATG GAGGACCTGC TGCGGCTCAA GGATTATCAG 
AAGCGCCTGG ACTTGTCCCT CCTGCGGCAG AGCAGCGACC CCATGCTAAG CGAGTTCAAG 
AACCTGGACA TCACCAAAAA GAAGCTGATC TACGAGGGTC CGCTGACGTG GCGGCTGACG 
AAGGACAAGG CTGTAGAGGT ACACGTGCTG CTGCTGGATG ACCTGCTGCT GCTGCTCCAG 
CGCCAGGACG ATCGGCTGCT GCTCAAGTCC CACAGCCGGA CGCTGACACC CACACCCGAC 
GGCAAGACCA TGCTGCGGCC GGTGCTGCGG CTCACCTCCG CCATAACCCG CGAGGTGGCT 
ACCGATCACA AAGCCTTCTA TGTCCTTTTT ACCTGGGACC AGGAGGCCCA GATATATGAG 
CTGGTGGCCC AGACGGTGTC GGAACGGAAG AACTGGTGTT CCCTCATCAC TGAAACTGCT 
GGATCCTTGA AGGTCCCTGC CCCCGCCTCT CGCCCCAAAC CCCGGCCCAG CCCAAGCAGC 
ACCCGAGAAC CCCTGCTCAG CAGCTCAGAG AATGGCAACG GTGGTGGCCG AGAGATGCCC 
CCAGCTGACG GCCAGACGGA GAGAATCCTC AGCACCCTCC TGCCCTACTG CAGGCCCCGC 
CCTGAGGGCC AGCTTGCTGC CAAGGCCCTT GAGAAAGTGC TGTCCCTGAA GCAGCTCCTG 
TTTCCCTCGG AGGAAGACAG TGGGGCAGGG CCTCCCCAGG AAGGGGATGG GGTCCCTGGG 
GGTGGCCCCC TGAACCCGAC ACAGACCCAC ACCCAGGAAA TTCGGGAAAA GCTGCTCAGC 
CTGGAGGAGA CCATGAAACA GCTGGAGGAG GTGGAGGAGG AATTTTGTCA CCTGCGACTC 
CTCCTGTCTC AGCTTGGGGA GAACAGCACC CCCCAGCCTG GGTGTACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_014120077.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013975552.1 Canis lupus familiaris Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe02096
Clone ID Related Accession (Same CDS sequence)	XM_014120077.1 , XM_014120077.2
Accession Version	XM_014120077.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rho guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120077.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGAAGGTG GAAGAGGCTG 
TCGGGGCGTC TGGGGCGCTC GGAGAGCCTG CGGGTGAGTG ACCGCCGCCG GCCTTCCCGG 
GGCAGCCTCG GGGCTAAGGG CCGGGGTGGG GGCCGCTCCC GGAGTGACGT GGACATGGAC 
CCCAGCTCTG CCACGGCCGT GCTTGGCCCT GCCCGACGAG CCACCCCTGA GCCTGGAGAC 
GAAGGGGACC CTGGGCGGTC AGGACTAGAG CTGGAACCAG AAGAGCCCCC TGGCTGGCGG 
GAGCTCATCC CCCCAGGCAC CCTGCACAGC CTGCCTAAGA GCCAGGTGAA GCGGCAGGAG 
GTCATCAGCG AGCTACTGGT GACAGAGGCT GCCCATGTGC GCATGCTCCG GGTGCTGCAT 
GACCTCTTCT ACCAGCCCAT GTTGGAAGGG AACTTCTTCT CCACGGAGGA TCTACAGAAC 
ATCTTCCCCA GCCTGGATGA GCTCATCGAG GTGCATTCCC TGTTCCTCGA TCGACTGATG 
AAGCGGAGGC AGGATAGTGG CTGCCTCATT GAGGAGATCG GAGATGTACT GCTGGCCCGG 
TTTGATGGTG CCGAAGGCAA ATGGTTCCAG AAAATCTCCT CCCGCTTCTG CAGCCGCCAG 
TCATTTGCCT TAGAGCAGCT CAAAGCCAAG CAGCGCAAGG ATCCTCGGTT CTGTGCCTTT 
GTGCAGGAGG CCGAGAGCCG CCCCCGGTGC CGCCGCCTGC AGCTGAAGGA TATGATCCCC 
ACGGAGATGC AGCGTCTGAC CAAGTACCCC CTGCTCCTGC AGAGCATTGG GCAGAACACA 
GAAGAGTCAG CAGAACGGGA GAAAGTAGAA CTGGCAGCTG AGTGCTGCCG GGAAATTCTG 
CACCATGTTA ACCAAGCCGT GCGTGACATG GAGGACCTGC TGCGGCTCAA GGATTATCAG 
AAGCGCCTGG ACTTGTCCCT CCTGCGGCAG AGCAGCGACC CCATGCTAAG CGAGTTCAAG 
AACCTGGACA TCACCAAAAA GAAGCTGATC TACGAGGGTC CGCTGACGTG GCGGCTGACG 
AAGGACAAGG CTGTAGAGGT ACACGTGCTG CTGCTGGATG ACCTGCTGCT GCTGCTCCAG 
CGCCAGGACG ATCGGCTGCT GCTCAAGTCC CACAGCCGGA CGCTGACACC CACACCCGAC 
GGCAAGACCA TGCTGCGGCC GGTGCTGCGG CTCACCTCCG CCATAACCCG CGAGGTGGCT 
ACCGATCACA AAGCCTTCTA TGTCCTTTTT ACCTGGGACC AGGAGGCCCA GATATATGAG 
CTGGTGGCCC AGACGGTGTC GGAACGGAAG AACTGGTGTT CCCTCATCAC TGAAACTGCT 
GGATCCTTGA AGGTCCCTGC CCCCGCCTCT CGCCCCAAAC CCCGGCCCAG CCCAAGCAGC 
ACCCGAGAAC CCCTGCTCAG CAGCTCAGAG AATGGCAACG GTGGTGGCCG AGAGATGCCC 
CCAGCTGACG GCCAGACGGA GAGAATCCTC AGCACCCTCC TGCCCTACTG CAGGCCCCGC 
CCTGAGGGCC AGCTTGCTGC CAAGGCCCTT GAGAAAGTGC TGTCCCTGAA GCAGCTCCTG 
TTTCCCTCGG AGGAAGACAG TGGGGCAGGG CCTCCCCAGG AAGGGGATGG GGTCCCTGGG 
GGTGGCCCCC TGAACCCGAC ACAGACCCAC ACCCAGGAAA TTCGGGAAAA GCTGCTCAGC 
CTGGAGGAGA CCATGAAACA GCTGGAGGAG GTGGAGGAGG AATTTTGTCA CCTGCGACTC 
CTCCTGTCTC AGCTTGGGGA GAACAGCACC CCCCAGCCTG GGTGTACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975552.1
CDS	129..3059
Translation	MEADNVARGLAPGPPRPGLVPVSIIGAEDEDFENELETNTEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTERRWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPARRATPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQKRLDLSLLRQSSDPMLSEFKNLDITKKKLIYEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDDRLLLKSHSRTLTPTPDGKTMLRPVLRLTSAITREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCSLITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGGREMPPADGQTERILSTLLPYCRPRPEGQLAAKALEKVLSLKQLLFPSEEDSGAGPPQEGDGVPGGGPLNPTQTHTQEIREKLLSLEETMKQLEEVEEEFCHLRLLLSQLGENSTPQPGCT

Target ORF information:

RefSeq Version	XM_014120077.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120077.2

ORF Insert Sequence:

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGAAGGTG GAAGAGGCTG 
TCGGGGCGTC TGGGGCGCTC GGAGAGCCTG CGGGTGAGTG ACCGCCGCCG GCCTTCCCGG 
GGCAGCCTCG GGGCTAAGGG CCGGGGTGGG GGCCGCTCCC GGAGTGACGT GGACATGGAC 
CCCAGCTCTG CCACGGCCGT GCTTGGCCCT GCCCGACGAG CCACCCCTGA GCCTGGAGAC 
GAAGGGGACC CTGGGCGGTC AGGACTAGAG CTGGAACCAG AAGAGCCCCC TGGCTGGCGG 
GAGCTCATCC CCCCAGGCAC CCTGCACAGC CTGCCTAAGA GCCAGGTGAA GCGGCAGGAG 
GTCATCAGCG AGCTACTGGT GACAGAGGCT GCCCATGTGC GCATGCTCCG GGTGCTGCAT 
GACCTCTTCT ACCAGCCCAT GTTGGAAGGG AACTTCTTCT CCACGGAGGA TCTACAGAAC 
ATCTTCCCCA GCCTGGATGA GCTCATCGAG GTGCATTCCC TGTTCCTCGA TCGACTGATG 
AAGCGGAGGC AGGATAGTGG CTGCCTCATT GAGGAGATCG GAGATGTACT GCTGGCCCGG 
TTTGATGGTG CCGAAGGCAA ATGGTTCCAG AAAATCTCCT CCCGCTTCTG CAGCCGCCAG 
TCATTTGCCT TAGAGCAGCT CAAAGCCAAG CAGCGCAAGG ATCCTCGGTT CTGTGCCTTT 
GTGCAGGAGG CCGAGAGCCG CCCCCGGTGC CGCCGCCTGC AGCTGAAGGA TATGATCCCC 
ACGGAGATGC AGCGTCTGAC CAAGTACCCC CTGCTCCTGC AGAGCATTGG GCAGAACACA 
GAAGAGTCAG CAGAACGGGA GAAAGTAGAA CTGGCAGCTG AGTGCTGCCG GGAAATTCTG 
CACCATGTTA ACCAAGCCGT GCGTGACATG GAGGACCTGC TGCGGCTCAA GGATTATCAG 
AAGCGCCTGG ACTTGTCCCT CCTGCGGCAG AGCAGCGACC CCATGCTAAG CGAGTTCAAG 
AACCTGGACA TCACCAAAAA GAAGCTGATC TACGAGGGTC CGCTGACGTG GCGGCTGACG 
AAGGACAAGG CTGTAGAGGT ACACGTGCTG CTGCTGGATG ACCTGCTGCT GCTGCTCCAG 
CGCCAGGACG ATCGGCTGCT GCTCAAGTCC CACAGCCGGA CGCTGACACC CACACCCGAC 
GGCAAGACCA TGCTGCGGCC GGTGCTGCGG CTCACCTCCG CCATAACCCG CGAGGTGGCT 
ACCGATCACA AAGCCTTCTA TGTCCTTTTT ACCTGGGACC AGGAGGCCCA GATATATGAG 
CTGGTGGCCC AGACGGTGTC GGAACGGAAG AACTGGTGTT CCCTCATCAC TGAAACTGCT 
GGATCCTTGA AGGTCCCTGC CCCCGCCTCT CGCCCCAAAC CCCGGCCCAG CCCAAGCAGC 
ACCCGAGAAC CCCTGCTCAG CAGCTCAGAG AATGGCAACG GTGGTGGCCG AGAGATGCCC 
CCAGCTGACG GCCAGACGGA GAGAATCCTC AGCACCCTCC TGCCCTACTG CAGGCCCCGC 
CCTGAGGGCC AGCTTGCTGC CAAGGCCCTT GAGAAAGTGC TGTCCCTGAA GCAGCTCCTG 
TTTCCCTCGG AGGAAGACAG TGGGGCAGGG CCTCCCCAGG AAGGGGATGG GGTCCCTGGG 
GGTGGCCCCC TGAACCCGAC ACAGACCCAC ACCCAGGAAA TTCGGGAAAA GCTGCTCAGC 
CTGGAGGAGA CCATGAAACA GCTGGAGGAG GTGGAGGAGG AATTTTGTCA CCTGCGACTC 
CTCCTGTCTC AGCTTGGGGA GAACAGCACC CCCCAGCCTG GGTGTACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_014120083.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013975558.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe02097
Clone ID Related Accession (Same CDS sequence)	XM_014120083.1 , XM_014120083.2
Accession Version	XM_014120083.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rho guanine nucleotide exchange factor 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGGCTGTC GGGGCGTCTG 
GGGCGCTCGG AGAGCCTGCG GGTGAGTGAC CGCCGCCGGC CTTCCCGGGG CAGCCTCGGG 
GCTAAGGGCC GGGGTGGGGG CCGCTCCCGG AGTGACGTGG ACATGGACCC CAGCTCTGCC 
ACGGCCGTGC TTGGCCCTGC CCGACGAGCC ACCCCTGAGC CTGGAGACGA AGGGGACCCT 
GGGCGGTCAG GACTAGAGCT GGAACCAGAA GAGCCCCCTG GCTGGCGGGA GCTCATCCCC 
CCAGGCACCC TGCACAGCCT GCCTAAGAGC CAGGTGAAGC GGCAGGAGGT CATCAGCGAG 
CTACTGGTGA CAGAGGCTGC CCATGTGCGC ATGCTCCGGG TGCTGCATGA CCTCTTCTAC 
CAGCCCATGT TGGAAGGGAA CTTCTTCTCC ACGGAGGATC TACAGAACAT CTTCCCCAGC 
CTGGATGAGC TCATCGAGGT GCATTCCCTG TTCCTCGATC GACTGATGAA GCGGAGGCAG 
GATAGTGGCT GCCTCATTGA GGAGATCGGA GATGTACTGC TGGCCCGGTT TGATGGTGCC 
GAAGGCAAAT GGTTCCAGAA AATCTCCTCC CGCTTCTGCA GCCGCCAGTC ATTTGCCTTA 
GAGCAGCTCA AAGCCAAGCA GCGCAAGGAT CCTCGGTTCT GTGCCTTTGT GCAGGAGGCC 
GAGAGCCGCC CCCGGTGCCG CCGCCTGCAG CTGAAGGATA TGATCCCCAC GGAGATGCAG 
CGTCTGACCA AGTACCCCCT GCTCCTGCAG AGCATTGGGC AGAACACAGA AGAGTCAGCA 
GAACGGGAGA AAGTAGAACT GGCAGCTGAG TGCTGCCGGG AAATTCTGCA CCATGTTAAC 
CAAGCCGTGC GTGACATGGA GGACCTGCTG CGGCTCAAGG ATTATCAGAA GCGCCTGGAC 
TTGTCCCTCC TGCGGCAGAG CAGCGACCCC ATGCTAAGCG AGTTCAAGAA CCTGGACATC 
ACCAAAAAGA AGCTGATCTA CGAGGGTCCG CTGACGTGGC GGCTGACGAA GGACAAGGCT 
GTAGAGGTAC ACGTGCTGCT GCTGGATGAC CTGCTGCTGC TGCTCCAGCG CCAGGACGAT 
CGGCTGCTGC TCAAGTCCCA CAGCCGGACG CTGACACCCA CACCCGACGG CAAGACCATG 
CTGCGGCCGG TGCTGCGGCT CACCTCCGCC ATAACCCGCG AGGTGGCTAC CGATCACAAA 
GCCTTCTATG TCCTTTTTAC CTGGGACCAG GAGGCCCAGA TATATGAGCT GGTGGCCCAG 
ACGGTGTCGG AACGGAAGAA CTGGTGTTCC CTCATCACTG AAACTGCTGG ATCCTTGAAG 
GTCCCTGCCC CCGCCTCTCG CCCCAAACCC CGGCCCAGCC CAAGCAGCAC CCGAGAACCC 
CTGCTCAGCA GCTCAGAGAA TGGCAACGGT GGTGGCCGAG AGATGCCCCC AGCTGACGGC 
CAGACGGAGA GAATCCTCAG CACCCTCCTG CCCTACTGCA GGCCCCGCCC TGAGGGCCAG 
CTTGCTGCCA AGGCCCTTGA GAAAGTGCTG TCCCTGAAGC AGCTCCTGTT TCCCTCGGAG 
GAAGACAGTG GGGCAGGGCC TCCCCAGGAA GGGGATGGGG TCCCTGGGGG TGGCCCCCTG 
AACCCGACAC AGACCCACAC CCAGGAAATT CGGGAAAAGC TGCTCAGCCT GGAGGAGACC 
ATGAAACAGC TGGAGGAGGT GGAGGAGGAA TTTTGTCACC TGCGACTCCT CCTGTCTCAG 
CTTGGGGAGA ACAGCACCCC CCAGCCTGGG TGTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975558.1
CDS	160..3078
Translation	MEADNVARGLAPGPPRPGLVPVSIIGAEDEDFENELETNTEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTERLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPARRATPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQKRLDLSLLRQSSDPMLSEFKNLDITKKKLIYEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDDRLLLKSHSRTLTPTPDGKTMLRPVLRLTSAITREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCSLITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGGREMPPADGQTERILSTLLPYCRPRPEGQLAAKALEKVLSLKQLLFPSEEDSGAGPPQEGDGVPGGGPLNPTQTHTQEIREKLLSLEETMKQLEEVEEEFCHLRLLLSQLGENSTPQPGCT

Target ORF information:

RefSeq Version	XM_014120083.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120083.1

ORF Insert Sequence:

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGGCTGTC GGGGCGTCTG 
GGGCGCTCGG AGAGCCTGCG GGTGAGTGAC CGCCGCCGGC CTTCCCGGGG CAGCCTCGGG 
GCTAAGGGCC GGGGTGGGGG CCGCTCCCGG AGTGACGTGG ACATGGACCC CAGCTCTGCC 
ACGGCCGTGC TTGGCCCTGC CCGACGAGCC ACCCCTGAGC CTGGAGACGA AGGGGACCCT 
GGGCGGTCAG GACTAGAGCT GGAACCAGAA GAGCCCCCTG GCTGGCGGGA GCTCATCCCC 
CCAGGCACCC TGCACAGCCT GCCTAAGAGC CAGGTGAAGC GGCAGGAGGT CATCAGCGAG 
CTACTGGTGA CAGAGGCTGC CCATGTGCGC ATGCTCCGGG TGCTGCATGA CCTCTTCTAC 
CAGCCCATGT TGGAAGGGAA CTTCTTCTCC ACGGAGGATC TACAGAACAT CTTCCCCAGC 
CTGGATGAGC TCATCGAGGT GCATTCCCTG TTCCTCGATC GACTGATGAA GCGGAGGCAG 
GATAGTGGCT GCCTCATTGA GGAGATCGGA GATGTACTGC TGGCCCGGTT TGATGGTGCC 
GAAGGCAAAT GGTTCCAGAA AATCTCCTCC CGCTTCTGCA GCCGCCAGTC ATTTGCCTTA 
GAGCAGCTCA AAGCCAAGCA GCGCAAGGAT CCTCGGTTCT GTGCCTTTGT GCAGGAGGCC 
GAGAGCCGCC CCCGGTGCCG CCGCCTGCAG CTGAAGGATA TGATCCCCAC GGAGATGCAG 
CGTCTGACCA AGTACCCCCT GCTCCTGCAG AGCATTGGGC AGAACACAGA AGAGTCAGCA 
GAACGGGAGA AAGTAGAACT GGCAGCTGAG TGCTGCCGGG AAATTCTGCA CCATGTTAAC 
CAAGCCGTGC GTGACATGGA GGACCTGCTG CGGCTCAAGG ATTATCAGAA GCGCCTGGAC 
TTGTCCCTCC TGCGGCAGAG CAGCGACCCC ATGCTAAGCG AGTTCAAGAA CCTGGACATC 
ACCAAAAAGA AGCTGATCTA CGAGGGTCCG CTGACGTGGC GGCTGACGAA GGACAAGGCT 
GTAGAGGTAC ACGTGCTGCT GCTGGATGAC CTGCTGCTGC TGCTCCAGCG CCAGGACGAT 
CGGCTGCTGC TCAAGTCCCA CAGCCGGACG CTGACACCCA CACCCGACGG CAAGACCATG 
CTGCGGCCGG TGCTGCGGCT CACCTCCGCC ATAACCCGCG AGGTGGCTAC CGATCACAAA 
GCCTTCTATG TCCTTTTTAC CTGGGACCAG GAGGCCCAGA TATATGAGCT GGTGGCCCAG 
ACGGTGTCGG AACGGAAGAA CTGGTGTTCC CTCATCACTG AAACTGCTGG ATCCTTGAAG 
GTCCCTGCCC CCGCCTCTCG CCCCAAACCC CGGCCCAGCC CAAGCAGCAC CCGAGAACCC 
CTGCTCAGCA GCTCAGAGAA TGGCAACGGT GGTGGCCGAG AGATGCCCCC AGCTGACGGC 
CAGACGGAGA GAATCCTCAG CACCCTCCTG CCCTACTGCA GGCCCCGCCC TGAGGGCCAG 
CTTGCTGCCA AGGCCCTTGA GAAAGTGCTG TCCCTGAAGC AGCTCCTGTT TCCCTCGGAG 
GAAGACAGTG GGGCAGGGCC TCCCCAGGAA GGGGATGGGG TCCCTGGGGG TGGCCCCCTG 
AACCCGACAC AGACCCACAC CCAGGAAATT CGGGAAAAGC TGCTCAGCCT GGAGGAGACC 
ATGAAACAGC TGGAGGAGGT GGAGGAGGAA TTTTGTCACC TGCGACTCCT CCTGTCTCAG 
CTTGGGGAGA ACAGCACCCC CCAGCCTGGG TGTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_014120083.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013975558.1 Canis lupus familiaris Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe02097
Clone ID Related Accession (Same CDS sequence)	XM_014120083.1 , XM_014120083.2
Accession Version	XM_014120083.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rho guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGGCTGTC GGGGCGTCTG 
GGGCGCTCGG AGAGCCTGCG GGTGAGTGAC CGCCGCCGGC CTTCCCGGGG CAGCCTCGGG 
GCTAAGGGCC GGGGTGGGGG CCGCTCCCGG AGTGACGTGG ACATGGACCC CAGCTCTGCC 
ACGGCCGTGC TTGGCCCTGC CCGACGAGCC ACCCCTGAGC CTGGAGACGA AGGGGACCCT 
GGGCGGTCAG GACTAGAGCT GGAACCAGAA GAGCCCCCTG GCTGGCGGGA GCTCATCCCC 
CCAGGCACCC TGCACAGCCT GCCTAAGAGC CAGGTGAAGC GGCAGGAGGT CATCAGCGAG 
CTACTGGTGA CAGAGGCTGC CCATGTGCGC ATGCTCCGGG TGCTGCATGA CCTCTTCTAC 
CAGCCCATGT TGGAAGGGAA CTTCTTCTCC ACGGAGGATC TACAGAACAT CTTCCCCAGC 
CTGGATGAGC TCATCGAGGT GCATTCCCTG TTCCTCGATC GACTGATGAA GCGGAGGCAG 
GATAGTGGCT GCCTCATTGA GGAGATCGGA GATGTACTGC TGGCCCGGTT TGATGGTGCC 
GAAGGCAAAT GGTTCCAGAA AATCTCCTCC CGCTTCTGCA GCCGCCAGTC ATTTGCCTTA 
GAGCAGCTCA AAGCCAAGCA GCGCAAGGAT CCTCGGTTCT GTGCCTTTGT GCAGGAGGCC 
GAGAGCCGCC CCCGGTGCCG CCGCCTGCAG CTGAAGGATA TGATCCCCAC GGAGATGCAG 
CGTCTGACCA AGTACCCCCT GCTCCTGCAG AGCATTGGGC AGAACACAGA AGAGTCAGCA 
GAACGGGAGA AAGTAGAACT GGCAGCTGAG TGCTGCCGGG AAATTCTGCA CCATGTTAAC 
CAAGCCGTGC GTGACATGGA GGACCTGCTG CGGCTCAAGG ATTATCAGAA GCGCCTGGAC 
TTGTCCCTCC TGCGGCAGAG CAGCGACCCC ATGCTAAGCG AGTTCAAGAA CCTGGACATC 
ACCAAAAAGA AGCTGATCTA CGAGGGTCCG CTGACGTGGC GGCTGACGAA GGACAAGGCT 
GTAGAGGTAC ACGTGCTGCT GCTGGATGAC CTGCTGCTGC TGCTCCAGCG CCAGGACGAT 
CGGCTGCTGC TCAAGTCCCA CAGCCGGACG CTGACACCCA CACCCGACGG CAAGACCATG 
CTGCGGCCGG TGCTGCGGCT CACCTCCGCC ATAACCCGCG AGGTGGCTAC CGATCACAAA 
GCCTTCTATG TCCTTTTTAC CTGGGACCAG GAGGCCCAGA TATATGAGCT GGTGGCCCAG 
ACGGTGTCGG AACGGAAGAA CTGGTGTTCC CTCATCACTG AAACTGCTGG ATCCTTGAAG 
GTCCCTGCCC CCGCCTCTCG CCCCAAACCC CGGCCCAGCC CAAGCAGCAC CCGAGAACCC 
CTGCTCAGCA GCTCAGAGAA TGGCAACGGT GGTGGCCGAG AGATGCCCCC AGCTGACGGC 
CAGACGGAGA GAATCCTCAG CACCCTCCTG CCCTACTGCA GGCCCCGCCC TGAGGGCCAG 
CTTGCTGCCA AGGCCCTTGA GAAAGTGCTG TCCCTGAAGC AGCTCCTGTT TCCCTCGGAG 
GAAGACAGTG GGGCAGGGCC TCCCCAGGAA GGGGATGGGG TCCCTGGGGG TGGCCCCCTG 
AACCCGACAC AGACCCACAC CCAGGAAATT CGGGAAAAGC TGCTCAGCCT GGAGGAGACC 
ATGAAACAGC TGGAGGAGGT GGAGGAGGAA TTTTGTCACC TGCGACTCCT CCTGTCTCAG 
CTTGGGGAGA ACAGCACCCC CCAGCCTGGG TGTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975558.1
CDS	129..3047
Translation	MEADNVARGLAPGPPRPGLVPVSIIGAEDEDFENELETNTEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTERLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPARRATPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQKRLDLSLLRQSSDPMLSEFKNLDITKKKLIYEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDDRLLLKSHSRTLTPTPDGKTMLRPVLRLTSAITREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCSLITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGGREMPPADGQTERILSTLLPYCRPRPEGQLAAKALEKVLSLKQLLFPSEEDSGAGPPQEGDGVPGGGPLNPTQTHTQEIREKLLSLEETMKQLEEVEEEFCHLRLLLSQLGENSTPQPGCT

Target ORF information:

RefSeq Version	XM_014120083.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120083.2

ORF Insert Sequence:

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGGCTGTC GGGGCGTCTG 
GGGCGCTCGG AGAGCCTGCG GGTGAGTGAC CGCCGCCGGC CTTCCCGGGG CAGCCTCGGG 
GCTAAGGGCC GGGGTGGGGG CCGCTCCCGG AGTGACGTGG ACATGGACCC CAGCTCTGCC 
ACGGCCGTGC TTGGCCCTGC CCGACGAGCC ACCCCTGAGC CTGGAGACGA AGGGGACCCT 
GGGCGGTCAG GACTAGAGCT GGAACCAGAA GAGCCCCCTG GCTGGCGGGA GCTCATCCCC 
CCAGGCACCC TGCACAGCCT GCCTAAGAGC CAGGTGAAGC GGCAGGAGGT CATCAGCGAG 
CTACTGGTGA CAGAGGCTGC CCATGTGCGC ATGCTCCGGG TGCTGCATGA CCTCTTCTAC 
CAGCCCATGT TGGAAGGGAA CTTCTTCTCC ACGGAGGATC TACAGAACAT CTTCCCCAGC 
CTGGATGAGC TCATCGAGGT GCATTCCCTG TTCCTCGATC GACTGATGAA GCGGAGGCAG 
GATAGTGGCT GCCTCATTGA GGAGATCGGA GATGTACTGC TGGCCCGGTT TGATGGTGCC 
GAAGGCAAAT GGTTCCAGAA AATCTCCTCC CGCTTCTGCA GCCGCCAGTC ATTTGCCTTA 
GAGCAGCTCA AAGCCAAGCA GCGCAAGGAT CCTCGGTTCT GTGCCTTTGT GCAGGAGGCC 
GAGAGCCGCC CCCGGTGCCG CCGCCTGCAG CTGAAGGATA TGATCCCCAC GGAGATGCAG 
CGTCTGACCA AGTACCCCCT GCTCCTGCAG AGCATTGGGC AGAACACAGA AGAGTCAGCA 
GAACGGGAGA AAGTAGAACT GGCAGCTGAG TGCTGCCGGG AAATTCTGCA CCATGTTAAC 
CAAGCCGTGC GTGACATGGA GGACCTGCTG CGGCTCAAGG ATTATCAGAA GCGCCTGGAC 
TTGTCCCTCC TGCGGCAGAG CAGCGACCCC ATGCTAAGCG AGTTCAAGAA CCTGGACATC 
ACCAAAAAGA AGCTGATCTA CGAGGGTCCG CTGACGTGGC GGCTGACGAA GGACAAGGCT 
GTAGAGGTAC ACGTGCTGCT GCTGGATGAC CTGCTGCTGC TGCTCCAGCG CCAGGACGAT 
CGGCTGCTGC TCAAGTCCCA CAGCCGGACG CTGACACCCA CACCCGACGG CAAGACCATG 
CTGCGGCCGG TGCTGCGGCT CACCTCCGCC ATAACCCGCG AGGTGGCTAC CGATCACAAA 
GCCTTCTATG TCCTTTTTAC CTGGGACCAG GAGGCCCAGA TATATGAGCT GGTGGCCCAG 
ACGGTGTCGG AACGGAAGAA CTGGTGTTCC CTCATCACTG AAACTGCTGG ATCCTTGAAG 
GTCCCTGCCC CCGCCTCTCG CCCCAAACCC CGGCCCAGCC CAAGCAGCAC CCGAGAACCC 
CTGCTCAGCA GCTCAGAGAA TGGCAACGGT GGTGGCCGAG AGATGCCCCC AGCTGACGGC 
CAGACGGAGA GAATCCTCAG CACCCTCCTG CCCTACTGCA GGCCCCGCCC TGAGGGCCAG 
CTTGCTGCCA AGGCCCTTGA GAAAGTGCTG TCCCTGAAGC AGCTCCTGTT TCCCTCGGAG 
GAAGACAGTG GGGCAGGGCC TCCCCAGGAA GGGGATGGGG TCCCTGGGGG TGGCCCCCTG 
AACCCGACAC AGACCCACAC CCAGGAAATT CGGGAAAAGC TGCTCAGCCT GGAGGAGACC 
ATGAAACAGC TGGAGGAGGT GGAGGAGGAA TTTTGTCACC TGCGACTCCT CCTGTCTCAG 
CTTGGGGAGA ACAGCACCCC CCAGCCTGGG TGTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_014120086.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013975561.1 Canis lupus familiaris Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe02098
Clone ID Related Accession (Same CDS sequence)	XM_014120086.2 , XM_014120086.1
Accession Version	XM_014120086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rho guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGCCCTGA GCCTGGAGAC 
GAAGGGGACC CTGGGCGGTC AGGACTAGAG CTGGAACCAG AAGAGCCCCC TGGCTGGCGG 
GAGCTCATCC CCCCAGGCAC CCTGCACAGC CTGCCTAAGA GCCAGGTGAA GCGGCAGGAG 
GTCATCAGCG AGCTACTGGT GACAGAGGCT GCCCATGTGC GCATGCTCCG GGTGCTGCAT 
GACCTCTTCT ACCAGCCCAT GTTGGAAGGG AACTTCTTCT CCACGGAGGA TCTACAGAAC 
ATCTTCCCCA GCCTGGATGA GCTCATCGAG GTGCATTCCC TGTTCCTCGA TCGACTGATG 
AAGCGGAGGC AGGATAGTGG CTGCCTCATT GAGGAGATCG GAGATGTACT GCTGGCCCGG 
TTTGATGGTG CCGAAGGCAA ATGGTTCCAG AAAATCTCCT CCCGCTTCTG CAGCCGCCAG 
TCATTTGCCT TAGAGCAGCT CAAAGCCAAG CAGCGCAAGG ATCCTCGGTT CTGTGCCTTT 
GTGCAGGAGG CCGAGAGCCG CCCCCGGTGC CGCCGCCTGC AGCTGAAGGA TATGATCCCC 
ACGGAGATGC AGCGTCTGAC CAAGTACCCC CTGCTCCTGC AGAGCATTGG GCAGAACACA 
GAAGAGTCAG CAGAACGGGA GAAAGTAGAA CTGGCAGCTG AGTGCTGCCG GGAAATTCTG 
CACCATGTTA ACCAAGCCGT GCGTGACATG GAGGACCTGC TGCGGCTCAA GGATTATCAG 
AAGCGCCTGG ACTTGTCCCT CCTGCGGCAG AGCAGCGACC CCATGCTAAG CGAGTTCAAG 
AACCTGGACA TCACCAAAAA GAAGCTGATC TACGAGGGTC CGCTGACGTG GCGGCTGACG 
AAGGACAAGG CTGTAGAGGT ACACGTGCTG CTGCTGGATG ACCTGCTGCT GCTGCTCCAG 
CGCCAGGACG ATCGGCTGCT GCTCAAGTCC CACAGCCGGA CGCTGACACC CACACCCGAC 
GGCAAGACCA TGCTGCGGCC GGTGCTGCGG CTCACCTCCG CCATAACCCG CGAGGTGGCT 
ACCGATCACA AAGCCTTCTA TGTCCTTTTT ACCTGGGACC AGGAGGCCCA GATATATGAG 
CTGGTGGCCC AGACGGTGTC GGAACGGAAG AACTGGTGTT CCCTCATCAC TGAAACTGCT 
GGATCCTTGA AGGTCCCTGC CCCCGCCTCT CGCCCCAAAC CCCGGCCCAG CCCAAGCAGC 
ACCCGAGAAC CCCTGCTCAG CAGCTCAGAG AATGGCAACG GTGGTGGCCG AGAGATGCCC 
CCAGCTGACG GCCAGACGGA GAGAATCCTC AGCACCCTCC TGCCCTACTG CAGGCCCCGC 
CCTGAGGGCC AGCTTGCTGC CAAGGCCCTT GAGAAAGTGC TGTCCCTGAA GCAGCTCCTG 
TTTCCCTCGG AGGAAGACAG TGGGGCAGGG CCTCCCCAGG AAGGGGATGG GGTCCCTGGG 
GGTGGCCCCC TGAACCCGAC ACAGACCCAC ACCCAGGAAA TTCGGGAAAA GCTGCTCAGC 
CTGGAGGAGA CCATGAAACA GCTGGAGGAG GTGGAGGAGG AATTTTGTCA CCTGCGACTC 
CTCCTGTCTC AGCTTGGGGA GAACAGCACC CCCCAGCCTG GGTGTACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975561.1
CDS	128..2878
Translation	MEADNVARGLAPGPPRPGLVPVSIIGAEDEDFENELETNTEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTESPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQKRLDLSLLRQSSDPMLSEFKNLDITKKKLIYEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDDRLLLKSHSRTLTPTPDGKTMLRPVLRLTSAITREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCSLITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGGREMPPADGQTERILSTLLPYCRPRPEGQLAAKALEKVLSLKQLLFPSEEDSGAGPPQEGDGVPGGGPLNPTQTHTQEIREKLLSLEETMKQLEEVEEEFCHLRLLLSQLGENSTPQPGCT

Target ORF information:

RefSeq Version	XM_014120086.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120086.2

ORF Insert Sequence:

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGCCCTGA GCCTGGAGAC 
GAAGGGGACC CTGGGCGGTC AGGACTAGAG CTGGAACCAG AAGAGCCCCC TGGCTGGCGG 
GAGCTCATCC CCCCAGGCAC CCTGCACAGC CTGCCTAAGA GCCAGGTGAA GCGGCAGGAG 
GTCATCAGCG AGCTACTGGT GACAGAGGCT GCCCATGTGC GCATGCTCCG GGTGCTGCAT 
GACCTCTTCT ACCAGCCCAT GTTGGAAGGG AACTTCTTCT CCACGGAGGA TCTACAGAAC 
ATCTTCCCCA GCCTGGATGA GCTCATCGAG GTGCATTCCC TGTTCCTCGA TCGACTGATG 
AAGCGGAGGC AGGATAGTGG CTGCCTCATT GAGGAGATCG GAGATGTACT GCTGGCCCGG 
TTTGATGGTG CCGAAGGCAA ATGGTTCCAG AAAATCTCCT CCCGCTTCTG CAGCCGCCAG 
TCATTTGCCT TAGAGCAGCT CAAAGCCAAG CAGCGCAAGG ATCCTCGGTT CTGTGCCTTT 
GTGCAGGAGG CCGAGAGCCG CCCCCGGTGC CGCCGCCTGC AGCTGAAGGA TATGATCCCC 
ACGGAGATGC AGCGTCTGAC CAAGTACCCC CTGCTCCTGC AGAGCATTGG GCAGAACACA 
GAAGAGTCAG CAGAACGGGA GAAAGTAGAA CTGGCAGCTG AGTGCTGCCG GGAAATTCTG 
CACCATGTTA ACCAAGCCGT GCGTGACATG GAGGACCTGC TGCGGCTCAA GGATTATCAG 
AAGCGCCTGG ACTTGTCCCT CCTGCGGCAG AGCAGCGACC CCATGCTAAG CGAGTTCAAG 
AACCTGGACA TCACCAAAAA GAAGCTGATC TACGAGGGTC CGCTGACGTG GCGGCTGACG 
AAGGACAAGG CTGTAGAGGT ACACGTGCTG CTGCTGGATG ACCTGCTGCT GCTGCTCCAG 
CGCCAGGACG ATCGGCTGCT GCTCAAGTCC CACAGCCGGA CGCTGACACC CACACCCGAC 
GGCAAGACCA TGCTGCGGCC GGTGCTGCGG CTCACCTCCG CCATAACCCG CGAGGTGGCT 
ACCGATCACA AAGCCTTCTA TGTCCTTTTT ACCTGGGACC AGGAGGCCCA GATATATGAG 
CTGGTGGCCC AGACGGTGTC GGAACGGAAG AACTGGTGTT CCCTCATCAC TGAAACTGCT 
GGATCCTTGA AGGTCCCTGC CCCCGCCTCT CGCCCCAAAC CCCGGCCCAG CCCAAGCAGC 
ACCCGAGAAC CCCTGCTCAG CAGCTCAGAG AATGGCAACG GTGGTGGCCG AGAGATGCCC 
CCAGCTGACG GCCAGACGGA GAGAATCCTC AGCACCCTCC TGCCCTACTG CAGGCCCCGC 
CCTGAGGGCC AGCTTGCTGC CAAGGCCCTT GAGAAAGTGC TGTCCCTGAA GCAGCTCCTG 
TTTCCCTCGG AGGAAGACAG TGGGGCAGGG CCTCCCCAGG AAGGGGATGG GGTCCCTGGG 
GGTGGCCCCC TGAACCCGAC ACAGACCCAC ACCCAGGAAA TTCGGGAAAA GCTGCTCAGC 
CTGGAGGAGA CCATGAAACA GCTGGAGGAG GTGGAGGAGG AATTTTGTCA CCTGCGACTC 
CTCCTGTCTC AGCTTGGGGA GAACAGCACC CCCCAGCCTG GGTGTACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_014120086.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013975561.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe02098
Clone ID Related Accession (Same CDS sequence)	XM_014120086.2 , XM_014120086.1
Accession Version	XM_014120086.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rho guanine nucleotide exchange factor 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGCCCTGA GCCTGGAGAC 
GAAGGGGACC CTGGGCGGTC AGGACTAGAG CTGGAACCAG AAGAGCCCCC TGGCTGGCGG 
GAGCTCATCC CCCCAGGCAC CCTGCACAGC CTGCCTAAGA GCCAGGTGAA GCGGCAGGAG 
GTCATCAGCG AGCTACTGGT GACAGAGGCT GCCCATGTGC GCATGCTCCG GGTGCTGCAT 
GACCTCTTCT ACCAGCCCAT GTTGGAAGGG AACTTCTTCT CCACGGAGGA TCTACAGAAC 
ATCTTCCCCA GCCTGGATGA GCTCATCGAG GTGCATTCCC TGTTCCTCGA TCGACTGATG 
AAGCGGAGGC AGGATAGTGG CTGCCTCATT GAGGAGATCG GAGATGTACT GCTGGCCCGG 
TTTGATGGTG CCGAAGGCAA ATGGTTCCAG AAAATCTCCT CCCGCTTCTG CAGCCGCCAG 
TCATTTGCCT TAGAGCAGCT CAAAGCCAAG CAGCGCAAGG ATCCTCGGTT CTGTGCCTTT 
GTGCAGGAGG CCGAGAGCCG CCCCCGGTGC CGCCGCCTGC AGCTGAAGGA TATGATCCCC 
ACGGAGATGC AGCGTCTGAC CAAGTACCCC CTGCTCCTGC AGAGCATTGG GCAGAACACA 
GAAGAGTCAG CAGAACGGGA GAAAGTAGAA CTGGCAGCTG AGTGCTGCCG GGAAATTCTG 
CACCATGTTA ACCAAGCCGT GCGTGACATG GAGGACCTGC TGCGGCTCAA GGATTATCAG 
AAGCGCCTGG ACTTGTCCCT CCTGCGGCAG AGCAGCGACC CCATGCTAAG CGAGTTCAAG 
AACCTGGACA TCACCAAAAA GAAGCTGATC TACGAGGGTC CGCTGACGTG GCGGCTGACG 
AAGGACAAGG CTGTAGAGGT ACACGTGCTG CTGCTGGATG ACCTGCTGCT GCTGCTCCAG 
CGCCAGGACG ATCGGCTGCT GCTCAAGTCC CACAGCCGGA CGCTGACACC CACACCCGAC 
GGCAAGACCA TGCTGCGGCC GGTGCTGCGG CTCACCTCCG CCATAACCCG CGAGGTGGCT 
ACCGATCACA AAGCCTTCTA TGTCCTTTTT ACCTGGGACC AGGAGGCCCA GATATATGAG 
CTGGTGGCCC AGACGGTGTC GGAACGGAAG AACTGGTGTT CCCTCATCAC TGAAACTGCT 
GGATCCTTGA AGGTCCCTGC CCCCGCCTCT CGCCCCAAAC CCCGGCCCAG CCCAAGCAGC 
ACCCGAGAAC CCCTGCTCAG CAGCTCAGAG AATGGCAACG GTGGTGGCCG AGAGATGCCC 
CCAGCTGACG GCCAGACGGA GAGAATCCTC AGCACCCTCC TGCCCTACTG CAGGCCCCGC 
CCTGAGGGCC AGCTTGCTGC CAAGGCCCTT GAGAAAGTGC TGTCCCTGAA GCAGCTCCTG 
TTTCCCTCGG AGGAAGACAG TGGGGCAGGG CCTCCCCAGG AAGGGGATGG GGTCCCTGGG 
GGTGGCCCCC TGAACCCGAC ACAGACCCAC ACCCAGGAAA TTCGGGAAAA GCTGCTCAGC 
CTGGAGGAGA CCATGAAACA GCTGGAGGAG GTGGAGGAGG AATTTTGTCA CCTGCGACTC 
CTCCTGTCTC AGCTTGGGGA GAACAGCACC CCCCAGCCTG GGTGTACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975561.1
CDS	158..2908
Translation	MEADNVARGLAPGPPRPGLVPVSIIGAEDEDFENELETNTEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTESPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQKRLDLSLLRQSSDPMLSEFKNLDITKKKLIYEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDDRLLLKSHSRTLTPTPDGKTMLRPVLRLTSAITREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCSLITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGGREMPPADGQTERILSTLLPYCRPRPEGQLAAKALEKVLSLKQLLFPSEEDSGAGPPQEGDGVPGGGPLNPTQTHTQEIREKLLSLEETMKQLEEVEEEFCHLRLLLSQLGENSTPQPGCT

Target ORF information:

RefSeq Version	XM_014120086.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120086.1

ORF Insert Sequence:

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGCCCTGA GCCTGGAGAC 
GAAGGGGACC CTGGGCGGTC AGGACTAGAG CTGGAACCAG AAGAGCCCCC TGGCTGGCGG 
GAGCTCATCC CCCCAGGCAC CCTGCACAGC CTGCCTAAGA GCCAGGTGAA GCGGCAGGAG 
GTCATCAGCG AGCTACTGGT GACAGAGGCT GCCCATGTGC GCATGCTCCG GGTGCTGCAT 
GACCTCTTCT ACCAGCCCAT GTTGGAAGGG AACTTCTTCT CCACGGAGGA TCTACAGAAC 
ATCTTCCCCA GCCTGGATGA GCTCATCGAG GTGCATTCCC TGTTCCTCGA TCGACTGATG 
AAGCGGAGGC AGGATAGTGG CTGCCTCATT GAGGAGATCG GAGATGTACT GCTGGCCCGG 
TTTGATGGTG CCGAAGGCAA ATGGTTCCAG AAAATCTCCT CCCGCTTCTG CAGCCGCCAG 
TCATTTGCCT TAGAGCAGCT CAAAGCCAAG CAGCGCAAGG ATCCTCGGTT CTGTGCCTTT 
GTGCAGGAGG CCGAGAGCCG CCCCCGGTGC CGCCGCCTGC AGCTGAAGGA TATGATCCCC 
ACGGAGATGC AGCGTCTGAC CAAGTACCCC CTGCTCCTGC AGAGCATTGG GCAGAACACA 
GAAGAGTCAG CAGAACGGGA GAAAGTAGAA CTGGCAGCTG AGTGCTGCCG GGAAATTCTG 
CACCATGTTA ACCAAGCCGT GCGTGACATG GAGGACCTGC TGCGGCTCAA GGATTATCAG 
AAGCGCCTGG ACTTGTCCCT CCTGCGGCAG AGCAGCGACC CCATGCTAAG CGAGTTCAAG 
AACCTGGACA TCACCAAAAA GAAGCTGATC TACGAGGGTC CGCTGACGTG GCGGCTGACG 
AAGGACAAGG CTGTAGAGGT ACACGTGCTG CTGCTGGATG ACCTGCTGCT GCTGCTCCAG 
CGCCAGGACG ATCGGCTGCT GCTCAAGTCC CACAGCCGGA CGCTGACACC CACACCCGAC 
GGCAAGACCA TGCTGCGGCC GGTGCTGCGG CTCACCTCCG CCATAACCCG CGAGGTGGCT 
ACCGATCACA AAGCCTTCTA TGTCCTTTTT ACCTGGGACC AGGAGGCCCA GATATATGAG 
CTGGTGGCCC AGACGGTGTC GGAACGGAAG AACTGGTGTT CCCTCATCAC TGAAACTGCT 
GGATCCTTGA AGGTCCCTGC CCCCGCCTCT CGCCCCAAAC CCCGGCCCAG CCCAAGCAGC 
ACCCGAGAAC CCCTGCTCAG CAGCTCAGAG AATGGCAACG GTGGTGGCCG AGAGATGCCC 
CCAGCTGACG GCCAGACGGA GAGAATCCTC AGCACCCTCC TGCCCTACTG CAGGCCCCGC 
CCTGAGGGCC AGCTTGCTGC CAAGGCCCTT GAGAAAGTGC TGTCCCTGAA GCAGCTCCTG 
TTTCCCTCGG AGGAAGACAG TGGGGCAGGG CCTCCCCAGG AAGGGGATGG GGTCCCTGGG 
GGTGGCCCCC TGAACCCGAC ACAGACCCAC ACCCAGGAAA TTCGGGAAAA GCTGCTCAGC 
CTGGAGGAGA CCATGAAACA GCTGGAGGAG GTGGAGGAGG AATTTTGTCA CCTGCGACTC 
CTCCTGTCTC AGCTTGGGGA GAACAGCACC CCCCAGCCTG GGTGTACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_014120090.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013975565.1 Canis lupus familiaris Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X4, mRNA.

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CloneID	OCe35012
Clone ID Related Accession (Same CDS sequence)	XM_014120090.2 , XM_014120090.1
Accession Version	XM_014120090.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rho guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120090.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGAAGGTG GAAGAGGCTG 
TCGGGGCGTC TGGGGCGCTC GGAGAGCCTG CGGGTGAGTG ACCGCCGCCG GCCTTCCCGG 
GGCAGCCTCG GGGCTAAGGG CCGGGGTGGG GGCCGCTCCC GGAGTGACGT GGACATGGAC 
CCCAGCTCTG CCACGGCCGT GCTTGGCCCT GCCCGACGAG CCACCCCTGA GCCTGGAGAC 
GAAGGGGACC CTGGGCGGTC AGGACTAGAG CTGGAACCAG AAGAGCCCCC TGGCTGGCGG 
GAGCTCATCC CCCCAGGCAC CCTGCACAGC CTGCCTAAGA GCCAGGTGAA GCGGCAGGAG 
GTCATCAGCG AGCTACTGGT GACAGAGGCT GCCCATGTGC GCATGCTCCG GGTGCTGCAT 
GACCTCTTCT ACCAGCCCAT GTTGGAAGGG AACTTCTTCT CCACGGAGGA TCTACAGAAC 
ATCTTCCCCA GCCTGGATGA GCTCATCGAG GTGCATTCCC TGTTCCTCGA TCGACTGATG 
AAGCGGAGGC AGGATAGTGG CTGCCTCATT GAGGAGATCG GAGATGTACT GCTGGCCCGG 
TTTGATGGTG CCGAAGGCAA ATGGTTCCAG AAAATCTCCT CCCGCTTCTG CAGCCGCCAG 
TCATTTGCCT TAGAGCAGCT CAAAGCCAAG CAGCGCAAGG ATCCTCGGTT CTGTGCCTTT 
GTGCAGGAGG CCGAGAGCCG CCCCCGGTGC CGCCGCCTGC AGCTGAAGGA TATGATCCCC 
ACGGAGATGC AGCGTCTGAC CAAGTACCCC CTGCTCCTGC AGAGCATTGG GCAGAACACA 
GAAGAGTCAG CAGAACGGGA GAAAGTAGAA CTGGCAGCTG AGTGCTGCCG GGAAATTCTG 
CACCATGTTA ACCAAGCCGT GCGTGACATG GAGGACCTGC TGGGTCACTG TGGGTTAAGC 
CCAGCCCTCG GCGTGTCCCC TGACTCTCCC TTCCCTGCCC CAGCGGCTCA AGGATTATCA 
GAAGCGCCTG GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA 
GAACCTGGAC ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC 
GAAGGACAAG GCTGTAGAGG TACACGTGCT GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975565.1
CDS	137..2398
Translation	MEADNVARGLAPGPPRPGLVPVSIIGAEDEDFENELETNTEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTERRWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPARRATPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLGHCGLSPALGVSPDSPFPAPAAQGLSEAPGLVPPAAEQRPHAKRVQEPGHHQKEADLRGSADVAADEGQGCRGTRAAAG

Target ORF information:

RefSeq Version	XM_014120090.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120090.2

ORF Insert Sequence:

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGAAGGTG GAAGAGGCTG 
TCGGGGCGTC TGGGGCGCTC GGAGAGCCTG CGGGTGAGTG ACCGCCGCCG GCCTTCCCGG 
GGCAGCCTCG GGGCTAAGGG CCGGGGTGGG GGCCGCTCCC GGAGTGACGT GGACATGGAC 
CCCAGCTCTG CCACGGCCGT GCTTGGCCCT GCCCGACGAG CCACCCCTGA GCCTGGAGAC 
GAAGGGGACC CTGGGCGGTC AGGACTAGAG CTGGAACCAG AAGAGCCCCC TGGCTGGCGG 
GAGCTCATCC CCCCAGGCAC CCTGCACAGC CTGCCTAAGA GCCAGGTGAA GCGGCAGGAG 
GTCATCAGCG AGCTACTGGT GACAGAGGCT GCCCATGTGC GCATGCTCCG GGTGCTGCAT 
GACCTCTTCT ACCAGCCCAT GTTGGAAGGG AACTTCTTCT CCACGGAGGA TCTACAGAAC 
ATCTTCCCCA GCCTGGATGA GCTCATCGAG GTGCATTCCC TGTTCCTCGA TCGACTGATG 
AAGCGGAGGC AGGATAGTGG CTGCCTCATT GAGGAGATCG GAGATGTACT GCTGGCCCGG 
TTTGATGGTG CCGAAGGCAA ATGGTTCCAG AAAATCTCCT CCCGCTTCTG CAGCCGCCAG 
TCATTTGCCT TAGAGCAGCT CAAAGCCAAG CAGCGCAAGG ATCCTCGGTT CTGTGCCTTT 
GTGCAGGAGG CCGAGAGCCG CCCCCGGTGC CGCCGCCTGC AGCTGAAGGA TATGATCCCC 
ACGGAGATGC AGCGTCTGAC CAAGTACCCC CTGCTCCTGC AGAGCATTGG GCAGAACACA 
GAAGAGTCAG CAGAACGGGA GAAAGTAGAA CTGGCAGCTG AGTGCTGCCG GGAAATTCTG 
CACCATGTTA ACCAAGCCGT GCGTGACATG GAGGACCTGC TGGGTCACTG TGGGTTAAGC 
CCAGCCCTCG GCGTGTCCCC TGACTCTCCC TTCCCTGCCC CAGCGGCTCA AGGATTATCA 
GAAGCGCCTG GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA 
GAACCTGGAC ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC 
GAAGGACAAG GCTGTAGAGG TACACGTGCT GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_014120090.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013975565.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe35012
Clone ID Related Accession (Same CDS sequence)	XM_014120090.2 , XM_014120090.1
Accession Version	XM_014120090.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rho guanine nucleotide exchange factor 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGAAGGTG GAAGAGGCTG 
TCGGGGCGTC TGGGGCGCTC GGAGAGCCTG CGGGTGAGTG ACCGCCGCCG GCCTTCCCGG 
GGCAGCCTCG GGGCTAAGGG CCGGGGTGGG GGCCGCTCCC GGAGTGACGT GGACATGGAC 
CCCAGCTCTG CCACGGCCGT GCTTGGCCCT GCCCGACGAG CCACCCCTGA GCCTGGAGAC 
GAAGGGGACC CTGGGCGGTC AGGACTAGAG CTGGAACCAG AAGAGCCCCC TGGCTGGCGG 
GAGCTCATCC CCCCAGGCAC CCTGCACAGC CTGCCTAAGA GCCAGGTGAA GCGGCAGGAG 
GTCATCAGCG AGCTACTGGT GACAGAGGCT GCCCATGTGC GCATGCTCCG GGTGCTGCAT 
GACCTCTTCT ACCAGCCCAT GTTGGAAGGG AACTTCTTCT CCACGGAGGA TCTACAGAAC 
ATCTTCCCCA GCCTGGATGA GCTCATCGAG GTGCATTCCC TGTTCCTCGA TCGACTGATG 
AAGCGGAGGC AGGATAGTGG CTGCCTCATT GAGGAGATCG GAGATGTACT GCTGGCCCGG 
TTTGATGGTG CCGAAGGCAA ATGGTTCCAG AAAATCTCCT CCCGCTTCTG CAGCCGCCAG 
TCATTTGCCT TAGAGCAGCT CAAAGCCAAG CAGCGCAAGG ATCCTCGGTT CTGTGCCTTT 
GTGCAGGAGG CCGAGAGCCG CCCCCGGTGC CGCCGCCTGC AGCTGAAGGA TATGATCCCC 
ACGGAGATGC AGCGTCTGAC CAAGTACCCC CTGCTCCTGC AGAGCATTGG GCAGAACACA 
GAAGAGTCAG CAGAACGGGA GAAAGTAGAA CTGGCAGCTG AGTGCTGCCG GGAAATTCTG 
CACCATGTTA ACCAAGCCGT GCGTGACATG GAGGACCTGC TGGGTCACTG TGGGTTAAGC 
CCAGCCCTCG GCGTGTCCCC TGACTCTCCC TTCCCTGCCC CAGCGGCTCA AGGATTATCA 
GAAGCGCCTG GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA 
GAACCTGGAC ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC 
GAAGGACAAG GCTGTAGAGG TACACGTGCT GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975565.1
CDS	162..2423
Translation	MEADNVARGLAPGPPRPGLVPVSIIGAEDEDFENELETNTEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLSSLGPKEAKKAFLDFCHCFLDKTAVLRVLVPPAVAFELDRTRPEVISEDLQRQFVQEVVQSQQAAVSRLLEDFRSKRLMGMTPWEQDLTQLEAWVGRDRASFEARERHLAERLLAHLEEMQHTISNDEEKSAAVVNAISLYMRHLGVRTKSGDKKSGMNFFRKKVMGNRRSDEPTKTKKGLSIFLDPARWNRGEPQASDFRHLKVETDAEKPGLTERKGGLGVPSRDRNVGAPGQDVPGVSLHPLPGDSPDREPGVDTPPELGDPPPQGPASLEPPGPSESTEEGPDTERRWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPARRATPEPGDEGDPGRSGLELEPEEPPGWRELIPPGTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMLEGNFFSTEDLQNIFPSLDELIEVHSLFLDRLMKRRQDSGCLIEEIGDVLLARFDGAEGKWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEESAEREKVELAAECCREILHHVNQAVRDMEDLLGHCGLSPALGVSPDSPFPAPAAQGLSEAPGLVPPAAEQRPHAKRVQEPGHHQKEADLRGSADVAADEGQGCRGTRAAAG

Target ORF information:

RefSeq Version	XM_014120090.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 1 (ARHGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120090.1

ORF Insert Sequence:

ATGGAAGCAG ACAACGTTGC CCGAGGGCTG GCACCAGGAC CACCGCGGCC TGGCCTGGTG 
CCAGTCAGCA TCATTGGGGC TGAGGACGAG GATTTTGAGA ATGAGCTGGA GACGAACACA 
GAGGAGCAAA ACAGCCAGTT CCAGAGCCTG GAGCAGGTGA AGCGGCGGCC TGCCCACCTC 
ATGGCCCTCT TGCAGCACGT CGCCCTGCAG TTTGAACCAG GACCTCTGCT CTGCTGCCTG 
CACGCGGACA TGCTGAGTTC CCTGGGCCCC AAGGAGGCCA AGAAGGCCTT CCTCGACTTC 
TGCCATTGCT TCCTGGACAA GACGGCGGTT TTGCGGGTGC TAGTCCCTCC CGCCGTCGCC 
TTTGAACTTG ACCGCACGCG GCCCGAGGTC ATCTCCGAGG ACTTGCAGCG GCAGTTTGTA 
CAGGAGGTAG TGCAGAGCCA GCAGGCAGCT GTTAGCCGGC TGCTGGAGGA CTTCCGCTCC 
AAGCGGCTCA TGGGCATGAC ACCCTGGGAG CAGGACCTGA CCCAGCTGGA GGCCTGGGTT 
GGGCGGGACC GTGCCAGCTT TGAGGCCCGG GAGCGGCACC TGGCTGAACG GCTGCTGGCC 
CACCTGGAGG AGATGCAACA CACCATCTCT AACGATGAGG AAAAGAGTGC CGCTGTGGTC 
AATGCCATCA GCCTGTACAT GCGCCACCTT GGGGTGCGGA CCAAGAGCGG AGACAAGAAG 
TCAGGGATGA ATTTCTTCCG CAAAAAGGTG ATGGGGAATC GGCGGTCAGA TGAGCCAACC 
AAGACCAAAA AAGGCCTGAG CATCTTCCTA GACCCTGCTC GCTGGAACCG GGGAGAGCCT 
CAGGCCTCGG ATTTCCGACA CCTCAAAGTC GAGACTGATG CTGAGAAACC AGGCCTTACG 
GAAAGGAAGG GAGGCCTGGG GGTGCCCTCC CGGGACCGGA ATGTTGGGGC TCCTGGGCAG 
GATGTCCCTG GAGTTTCTCT GCACCCTCTG CCCGGGGACA GCCCTGACCG GGAACCAGGT 
GTTGACACCC CGCCAGAGCT GGGGGACCCA CCCCCCCAGG GCCCGGCCAG CCTGGAGCCC 
CCGGGGCCCT CGGAGAGCAC TGAGGAGGGT CCTGACACAG AGAGAAGGTG GAAGAGGCTG 
TCGGGGCGTC TGGGGCGCTC GGAGAGCCTG CGGGTGAGTG ACCGCCGCCG GCCTTCCCGG 
GGCAGCCTCG GGGCTAAGGG CCGGGGTGGG GGCCGCTCCC GGAGTGACGT GGACATGGAC 
CCCAGCTCTG CCACGGCCGT GCTTGGCCCT GCCCGACGAG CCACCCCTGA GCCTGGAGAC 
GAAGGGGACC CTGGGCGGTC AGGACTAGAG CTGGAACCAG AAGAGCCCCC TGGCTGGCGG 
GAGCTCATCC CCCCAGGCAC CCTGCACAGC CTGCCTAAGA GCCAGGTGAA GCGGCAGGAG 
GTCATCAGCG AGCTACTGGT GACAGAGGCT GCCCATGTGC GCATGCTCCG GGTGCTGCAT 
GACCTCTTCT ACCAGCCCAT GTTGGAAGGG AACTTCTTCT CCACGGAGGA TCTACAGAAC 
ATCTTCCCCA GCCTGGATGA GCTCATCGAG GTGCATTCCC TGTTCCTCGA TCGACTGATG 
AAGCGGAGGC AGGATAGTGG CTGCCTCATT GAGGAGATCG GAGATGTACT GCTGGCCCGG 
TTTGATGGTG CCGAAGGCAA ATGGTTCCAG AAAATCTCCT CCCGCTTCTG CAGCCGCCAG 
TCATTTGCCT TAGAGCAGCT CAAAGCCAAG CAGCGCAAGG ATCCTCGGTT CTGTGCCTTT 
GTGCAGGAGG CCGAGAGCCG CCCCCGGTGC CGCCGCCTGC AGCTGAAGGA TATGATCCCC 
ACGGAGATGC AGCGTCTGAC CAAGTACCCC CTGCTCCTGC AGAGCATTGG GCAGAACACA 
GAAGAGTCAG CAGAACGGGA GAAAGTAGAA CTGGCAGCTG AGTGCTGCCG GGAAATTCTG 
CACCATGTTA ACCAAGCCGT GCGTGACATG GAGGACCTGC TGGGTCACTG TGGGTTAAGC 
CCAGCCCTCG GCGTGTCCCC TGACTCTCCC TTCCCTGCCC CAGCGGCTCA AGGATTATCA 
GAAGCGCCTG GACTTGTCCC TCCTGCGGCA GAGCAGCGAC CCCATGCTAA GCGAGTTCAA 
GAACCTGGAC ATCACCAAAA AGAAGCTGAT CTACGAGGGT CCGCTGACGT GGCGGCTGAC 
GAAGGACAAG GCTGTAGAGG TACACGTGCT GCTGCTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ARHGEF1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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