LIG1 cDNA ORF clone, Canis lupus familiaris(dog)

The following LIG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe01931 XM_014119239.2
Latest version!
Canis lupus familiaris DNA ligase 1 (LIG1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe01931 XM_005616372.3
Latest version!
Canis lupus familiaris DNA ligase 1 (LIG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe01931 XM_005616373.2
Latest version!
Canis lupus familiaris DNA ligase 1 (LIG1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe01931 XM_014119238.2
Latest version!
Canis lupus familiaris DNA ligase 1 (LIG1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe01932 XM_005616374.3
Latest version!
Canis lupus familiaris DNA ligase 1 (LIG1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe01933 XM_005616375.3
Latest version!
Canis lupus familiaris DNA ligase 1 (LIG1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe286225 XM_022424455.1
Latest version!
Canis lupus familiaris DNA ligase 1 (LIG1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
OCe01932 XM_005616374.2 Canis lupus familiaris ligase I, DNA, ATP-dependent (LIG1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe01933 XM_005616375.2 Canis lupus familiaris ligase I, DNA, ATP-dependent (LIG1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe01931 XM_005616372.2 Canis lupus familiaris ligase I, DNA, ATP-dependent (LIG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe01931 XM_014119238.1 Canis lupus familiaris ligase I, DNA, ATP-dependent (LIG1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe01931 XM_014119239.1 Canis lupus familiaris ligase I, DNA, ATP-dependent (LIG1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe01931 XM_005616373.1 Canis lupus familiaris ligase I, DNA, ATP-dependent (LIG1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe01931
    Clone ID Related Accession (Same CDS sequence) XM_005616372.3 , XM_014119239.1 , XM_014119238.1 , XM_005616372.2 , XM_014119238.2 , XM_014119239.2 , XM_005616373.1 , XM_005616373.2
    Accession Version XM_005616372.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA ligase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005616372.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616429.1
    CDS195..2936
    Translation

    Target ORF information:

    RefSeq Version XM_005616372.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA ligase 1 (LIG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616372.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01931
    Clone ID Related Accession (Same CDS sequence) XM_005616372.3 , XM_014119239.1 , XM_014119238.1 , XM_005616372.2 , XM_014119238.2 , XM_014119239.2 , XM_005616373.1 , XM_005616373.2
    Accession Version XM_014119239.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA ligase 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974714.1
    CDS97..2838
    Translation

    Target ORF information:

    RefSeq Version XM_014119239.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ligase I, DNA, ATP-dependent (LIG1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119239.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01931
    Clone ID Related Accession (Same CDS sequence) XM_005616372.3 , XM_014119239.1 , XM_014119238.1 , XM_005616372.2 , XM_014119238.2 , XM_014119239.2 , XM_005616373.1 , XM_005616373.2
    Accession Version XM_014119238.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA ligase 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974713.1
    CDS170..2911
    Translation

    Target ORF information:

    RefSeq Version XM_014119238.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ligase I, DNA, ATP-dependent (LIG1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119238.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01931
    Clone ID Related Accession (Same CDS sequence) XM_005616372.3 , XM_014119239.1 , XM_014119238.1 , XM_005616372.2 , XM_014119238.2 , XM_014119239.2 , XM_005616373.1 , XM_005616373.2
    Accession Version XM_005616372.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA ligase 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005616372.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616429.1
    CDS174..2915
    Translation

    Target ORF information:

    RefSeq Version XM_005616372.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ligase I, DNA, ATP-dependent (LIG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616372.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01931
    Clone ID Related Accession (Same CDS sequence) XM_005616372.3 , XM_014119239.1 , XM_014119238.1 , XM_005616372.2 , XM_014119238.2 , XM_014119239.2 , XM_005616373.1 , XM_005616373.2
    Accession Version XM_014119238.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA ligase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014119238.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974713.1
    CDS171..2912
    Translation

    Target ORF information:

    RefSeq Version XM_014119238.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA ligase 1 (LIG1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119238.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01931
    Clone ID Related Accession (Same CDS sequence) XM_005616372.3 , XM_014119239.1 , XM_014119238.1 , XM_005616372.2 , XM_014119238.2 , XM_014119239.2 , XM_005616373.1 , XM_005616373.2
    Accession Version XM_014119239.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA ligase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014119239.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974714.1
    CDS70..2811
    Translation

    Target ORF information:

    RefSeq Version XM_014119239.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA ligase 1 (LIG1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119239.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01931
    Clone ID Related Accession (Same CDS sequence) XM_005616372.3 , XM_014119239.1 , XM_014119238.1 , XM_005616372.2 , XM_014119238.2 , XM_014119239.2 , XM_005616373.1 , XM_005616373.2
    Accession Version XM_005616373.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA ligase 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616430.1
    CDS133..2874
    Translation

    Target ORF information:

    RefSeq Version XM_005616373.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ligase I, DNA, ATP-dependent (LIG1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01931
    Clone ID Related Accession (Same CDS sequence) XM_005616372.3 , XM_014119239.1 , XM_014119238.1 , XM_005616372.2 , XM_014119238.2 , XM_014119239.2 , XM_005616373.1 , XM_005616373.2
    Accession Version XM_005616373.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA ligase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005616373.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616430.1
    CDS143..2884
    Translation

    Target ORF information:

    RefSeq Version XM_005616373.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA ligase 1 (LIG1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616373.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCCA GAGCCCCCGG TGGATAGCAC GTTGGAGCCT
    GCGGTGTCTG GGAAGCAGGA GGCTCAGAAC GAGGACTTGG CTAAGCCGCC CACCAGAGCC
    CCCAAGACGG TCAGCAGCTT CTTTGCCCCG CGGAAACCGG CAACCAAGAA AGAAGGGAAA
    GAAGAGGACC CTGGCACTCC AAGGCAGGAG GAGACCAAGG GACCCCTGGA CCCTCCCAGC
    TACAATCCTG CCAAGAACAA CTACCATCCC ATTGATGATG CATGCTGGAA GCCAGGCCAG
    AAGGTCCCTT ACCTGGCTGT GGCCCGGACA TTTGAGAAGA TTGAGGAAGT TTCTGCTCGG
    CTCCGGATGG TGGAGACGCT GAGCAACTTG CTGCGCTCTG TGGTGGCCCT GTCACCTCCG
    GACCTCCTTC CTATCCTCTA CCTCAGCCTC AACCGTCTTG GGCCACCCCA GCAGGGTCTG
    GAACTTGGTG TCGGTGATGG TGTTCTCCTC AAGGCGGTGG CCCAGGCCAC AGGGCGGCAG
    CTGGACTCTG TTAAGGCAGA GGCAGCTGAG AAGGGTGACG TGGGGCTGGT GGCCGAAAAC
    AGCCGCAGTA CCCAGAGGCT CATGCTGCCC CCACCTGCTC TCACCGCTGC CGGGGTCTTC
    GCCAAGTTCC GAGACATTGC CCGGCTTGCT GGCAGTGCCT CCACAGCCAA GAAGATAGAT
    GTCATCAAAG GCCTCTTCGT CGCCTGCCGC CACTCAGAAG CCCGATTCAT TGCCAGGGCC
    CTGAGTGGAC GGCTGCGCCT CGGGCTGGCA GAGCAGTCGG TGCTGGCTGC CCTCGCCCAG
    GCCGTGAGCC TCACGCCCCC AGGCCAAGAA TTCCCCCCGG CCGTTGTGGA TGCTGGGAAG
    GGCCGGACAG CAGAAGCCCG AAAGACGTGG CTGGAGGAGC AAGGCATGAT CCTGAAGCAG
    ACGTTCTGCG AGGTGCCCGA CCTGGACCGG ATCATCCCTG TACTGCTGGA GCATGGCCTG
    GAGCACCTCC CTGAGCACTG CAGGTTGAGC CCAGGGGTTC CACTGAAACC AATGCTGGCG
    CACCCCACCC GGGGTGTCAG CGAGGTCCTA AAACGTTTTG AAGAGGCAGC TTTCACCTGC
    GAGTACAAAT ACGATGGGCA GAGGGCACAG ATCCACGTTC TGGAAGGTGG GGAGGTGAAG
    ATCTTCAGCA GGAATCAGGA AGACAATACC GGAAAGTACC CAGACATCAT CAGCCGCATT
    CCCAAGATTA AACTCCCGTC AGTCACGTCT TTCATTCTGG ACACAGAAGC TGTGGCATGG
    GACCGAGAAA AGAAGCAGAT CCAGCCATTC CAAGTGCTCA CCACCCGCAA ACGCAAGGAG
    GTGGATGCAG CAGAGATCCA GGTGCAGGTG TGTCTATATG CCTTCGACCT CATTTACCTC
    AATGGAGAGT CCCTGGTACG TGAGCCTCTT TCCCGACGCC GGCAGCTGCT CCGGGAGAAC
    TTTGTGGAGA CGGAGGGCGA GTTTGTGTTC GCCACCTCCC TGGACACCAA GGACACGGAC
    CAAATTGCCG AGTTCTTAGA GCAGTCTGTG AAAGACTCCT GTGAGGGGCT GATGGTGAAA
    ACTCTGGACG TTGATGCCAC CTATGAGATC GCCAAGAGAT CCCACAACTG GCTCAAGCTG
    AAGAAGGACT ACCTCGATGG TGTGGGCGAC ACCCTGGACC TCGTGGTGAT TGGCGCCTAC
    CTGGGCCGTG GCAAACGGGC TGGCCGCTAT GGGGGCTTCC TACTGGCTGC CTACGACGAG
    GAGAGTGAGG AGCTCCAGGC CATATGCAAG CTCGGAACTG GCTTCAGTGA CGAGGAACTG
    GAGGAGCACT ACCAGAGGCT CCAGGCCCTG GTGCTGCCCA CCCCACGCTC CTATGTCCGG
    GTCGACGGTG CCGTGGCCCC CGATCACTGG CTGGACCCCA GTGCCGTGTG GGAGGTGAAG
    TGTGCGGATC TGTCCTTGTC GCCCATCTAC CCTGCTGCCC GGGGCCTGGT GGATGGTGAG
    AAGGGGATCT CCCTTCGCTT TCCTCGGTTT ATCCGGGTCC GTGAAGACAA GAAGCCAGAG
    GAGGCCACCA CCAGTGGCCA GGTGGCCTGC CTGTACATGA AGCAGAGTCA GATTCAGAAC
    CAGCAGGGCG CGGACTTAGA CTCTGACTCA GATTTCTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01932
    Clone ID Related Accession (Same CDS sequence) XM_005616374.3 , XM_005616374.2
    Accession Version XM_005616374.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA ligase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005616374.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCAG CCCCCGGTGG ATAGCACGTT GGAGCCTGCG
    GTGTCTGGGA AGCAGGAGGC TCAGAACGAG GACTTGGCTA AGCCGCCCAC CAGAGCCCCC
    AAGACGGTCA GCAGCTTCTT TGCCCCGCGG AAACCGGCAA CCAAGAAAGA AGGGAAAGAA
    GAGGACCCTG GCACTCCAAG GCAGGAGGAG ACCAAGGGAC CCCTGGACCC TCCCAGCTAC
    AATCCTGCCA AGAACAACTA CCATCCCATT GATGATGCAT GCTGGAAGCC AGGCCAGAAG
    GTCCCTTACC TGGCTGTGGC CCGGACATTT GAGAAGATTG AGGAAGTTTC TGCTCGGCTC
    CGGATGGTGG AGACGCTGAG CAACTTGCTG CGCTCTGTGG TGGCCCTGTC ACCTCCGGAC
    CTCCTTCCTA TCCTCTACCT CAGCCTCAAC CGTCTTGGGC CACCCCAGCA GGGTCTGGAA
    CTTGGTGTCG GTGATGGTGT TCTCCTCAAG GCGGTGGCCC AGGCCACAGG GCGGCAGCTG
    GACTCTGTTA AGGCAGAGGC AGCTGAGAAG GGTGACGTGG GGCTGGTGGC CGAAAACAGC
    CGCAGTACCC AGAGGCTCAT GCTGCCCCCA CCTGCTCTCA CCGCTGCCGG GGTCTTCGCC
    AAGTTCCGAG ACATTGCCCG GCTTGCTGGC AGTGCCTCCA CAGCCAAGAA GATAGATGTC
    ATCAAAGGCC TCTTCGTCGC CTGCCGCCAC TCAGAAGCCC GATTCATTGC CAGGGCCCTG
    AGTGGACGGC TGCGCCTCGG GCTGGCAGAG CAGTCGGTGC TGGCTGCCCT CGCCCAGGCC
    GTGAGCCTCA CGCCCCCAGG CCAAGAATTC CCCCCGGCCG TTGTGGATGC TGGGAAGGGC
    CGGACAGCAG AAGCCCGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACG
    TTCTGCGAGG TGCCCGACCT GGACCGGATC ATCCCTGTAC TGCTGGAGCA TGGCCTGGAG
    CACCTCCCTG AGCACTGCAG GTTGAGCCCA GGGGTTCCAC TGAAACCAAT GCTGGCGCAC
    CCCACCCGGG GTGTCAGCGA GGTCCTAAAA CGTTTTGAAG AGGCAGCTTT CACCTGCGAG
    TACAAATACG ATGGGCAGAG GGCACAGATC CACGTTCTGG AAGGTGGGGA GGTGAAGATC
    TTCAGCAGGA ATCAGGAAGA CAATACCGGA AAGTACCCAG ACATCATCAG CCGCATTCCC
    AAGATTAAAC TCCCGTCAGT CACGTCTTTC ATTCTGGACA CAGAAGCTGT GGCATGGGAC
    CGAGAAAAGA AGCAGATCCA GCCATTCCAA GTGCTCACCA CCCGCAAACG CAAGGAGGTG
    GATGCAGCAG AGATCCAGGT GCAGGTGTGT CTATATGCCT TCGACCTCAT TTACCTCAAT
    GGAGAGTCCC TGGTACGTGA GCCTCTTTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTT
    GTGGAGACGG AGGGCGAGTT TGTGTTCGCC ACCTCCCTGG ACACCAAGGA CACGGACCAA
    ATTGCCGAGT TCTTAGAGCA GTCTGTGAAA GACTCCTGTG AGGGGCTGAT GGTGAAAACT
    CTGGACGTTG ATGCCACCTA TGAGATCGCC AAGAGATCCC ACAACTGGCT CAAGCTGAAG
    AAGGACTACC TCGATGGTGT GGGCGACACC CTGGACCTCG TGGTGATTGG CGCCTACCTG
    GGCCGTGGCA AACGGGCTGG CCGCTATGGG GGCTTCCTAC TGGCTGCCTA CGACGAGGAG
    AGTGAGGAGC TCCAGGCCAT ATGCAAGCTC GGAACTGGCT TCAGTGACGA GGAACTGGAG
    GAGCACTACC AGAGGCTCCA GGCCCTGGTG CTGCCCACCC CACGCTCCTA TGTCCGGGTC
    GACGGTGCCG TGGCCCCCGA TCACTGGCTG GACCCCAGTG CCGTGTGGGA GGTGAAGTGT
    GCGGATCTGT CCTTGTCGCC CATCTACCCT GCTGCCCGGG GCCTGGTGGA TGGTGAGAAG
    GGGATCTCCC TTCGCTTTCC TCGGTTTATC CGGGTCCGTG AAGACAAGAA GCCAGAGGAG
    GCCACCACCA GTGGCCAGGT GGCCTGCCTG TACATGAAGC AGAGTCAGAT TCAGAACCAG
    CAGGGCGCGG ACTTAGACTC TGACTCAGAT TTCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616431.1
    CDS194..2932
    Translation

    Target ORF information:

    RefSeq Version XM_005616374.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA ligase 1 (LIG1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616374.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCAG CCCCCGGTGG ATAGCACGTT GGAGCCTGCG
    GTGTCTGGGA AGCAGGAGGC TCAGAACGAG GACTTGGCTA AGCCGCCCAC CAGAGCCCCC
    AAGACGGTCA GCAGCTTCTT TGCCCCGCGG AAACCGGCAA CCAAGAAAGA AGGGAAAGAA
    GAGGACCCTG GCACTCCAAG GCAGGAGGAG ACCAAGGGAC CCCTGGACCC TCCCAGCTAC
    AATCCTGCCA AGAACAACTA CCATCCCATT GATGATGCAT GCTGGAAGCC AGGCCAGAAG
    GTCCCTTACC TGGCTGTGGC CCGGACATTT GAGAAGATTG AGGAAGTTTC TGCTCGGCTC
    CGGATGGTGG AGACGCTGAG CAACTTGCTG CGCTCTGTGG TGGCCCTGTC ACCTCCGGAC
    CTCCTTCCTA TCCTCTACCT CAGCCTCAAC CGTCTTGGGC CACCCCAGCA GGGTCTGGAA
    CTTGGTGTCG GTGATGGTGT TCTCCTCAAG GCGGTGGCCC AGGCCACAGG GCGGCAGCTG
    GACTCTGTTA AGGCAGAGGC AGCTGAGAAG GGTGACGTGG GGCTGGTGGC CGAAAACAGC
    CGCAGTACCC AGAGGCTCAT GCTGCCCCCA CCTGCTCTCA CCGCTGCCGG GGTCTTCGCC
    AAGTTCCGAG ACATTGCCCG GCTTGCTGGC AGTGCCTCCA CAGCCAAGAA GATAGATGTC
    ATCAAAGGCC TCTTCGTCGC CTGCCGCCAC TCAGAAGCCC GATTCATTGC CAGGGCCCTG
    AGTGGACGGC TGCGCCTCGG GCTGGCAGAG CAGTCGGTGC TGGCTGCCCT CGCCCAGGCC
    GTGAGCCTCA CGCCCCCAGG CCAAGAATTC CCCCCGGCCG TTGTGGATGC TGGGAAGGGC
    CGGACAGCAG AAGCCCGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACG
    TTCTGCGAGG TGCCCGACCT GGACCGGATC ATCCCTGTAC TGCTGGAGCA TGGCCTGGAG
    CACCTCCCTG AGCACTGCAG GTTGAGCCCA GGGGTTCCAC TGAAACCAAT GCTGGCGCAC
    CCCACCCGGG GTGTCAGCGA GGTCCTAAAA CGTTTTGAAG AGGCAGCTTT CACCTGCGAG
    TACAAATACG ATGGGCAGAG GGCACAGATC CACGTTCTGG AAGGTGGGGA GGTGAAGATC
    TTCAGCAGGA ATCAGGAAGA CAATACCGGA AAGTACCCAG ACATCATCAG CCGCATTCCC
    AAGATTAAAC TCCCGTCAGT CACGTCTTTC ATTCTGGACA CAGAAGCTGT GGCATGGGAC
    CGAGAAAAGA AGCAGATCCA GCCATTCCAA GTGCTCACCA CCCGCAAACG CAAGGAGGTG
    GATGCAGCAG AGATCCAGGT GCAGGTGTGT CTATATGCCT TCGACCTCAT TTACCTCAAT
    GGAGAGTCCC TGGTACGTGA GCCTCTTTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTT
    GTGGAGACGG AGGGCGAGTT TGTGTTCGCC ACCTCCCTGG ACACCAAGGA CACGGACCAA
    ATTGCCGAGT TCTTAGAGCA GTCTGTGAAA GACTCCTGTG AGGGGCTGAT GGTGAAAACT
    CTGGACGTTG ATGCCACCTA TGAGATCGCC AAGAGATCCC ACAACTGGCT CAAGCTGAAG
    AAGGACTACC TCGATGGTGT GGGCGACACC CTGGACCTCG TGGTGATTGG CGCCTACCTG
    GGCCGTGGCA AACGGGCTGG CCGCTATGGG GGCTTCCTAC TGGCTGCCTA CGACGAGGAG
    AGTGAGGAGC TCCAGGCCAT ATGCAAGCTC GGAACTGGCT TCAGTGACGA GGAACTGGAG
    GAGCACTACC AGAGGCTCCA GGCCCTGGTG CTGCCCACCC CACGCTCCTA TGTCCGGGTC
    GACGGTGCCG TGGCCCCCGA TCACTGGCTG GACCCCAGTG CCGTGTGGGA GGTGAAGTGT
    GCGGATCTGT CCTTGTCGCC CATCTACCCT GCTGCCCGGG GCCTGGTGGA TGGTGAGAAG
    GGGATCTCCC TTCGCTTTCC TCGGTTTATC CGGGTCCGTG AAGACAAGAA GCCAGAGGAG
    GCCACCACCA GTGGCCAGGT GGCCTGCCTG TACATGAAGC AGAGTCAGAT TCAGAACCAG
    CAGGGCGCGG ACTTAGACTC TGACTCAGAT TTCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01932
    Clone ID Related Accession (Same CDS sequence) XM_005616374.3 , XM_005616374.2
    Accession Version XM_005616374.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA ligase 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005616374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCAG CCCCCGGTGG ATAGCACGTT GGAGCCTGCG
    GTGTCTGGGA AGCAGGAGGC TCAGAACGAG GACTTGGCTA AGCCGCCCAC CAGAGCCCCC
    AAGACGGTCA GCAGCTTCTT TGCCCCGCGG AAACCGGCAA CCAAGAAAGA AGGGAAAGAA
    GAGGACCCTG GCACTCCAAG GCAGGAGGAG ACCAAGGGAC CCCTGGACCC TCCCAGCTAC
    AATCCTGCCA AGAACAACTA CCATCCCATT GATGATGCAT GCTGGAAGCC AGGCCAGAAG
    GTCCCTTACC TGGCTGTGGC CCGGACATTT GAGAAGATTG AGGAAGTTTC TGCTCGGCTC
    CGGATGGTGG AGACGCTGAG CAACTTGCTG CGCTCTGTGG TGGCCCTGTC ACCTCCGGAC
    CTCCTTCCTA TCCTCTACCT CAGCCTCAAC CGTCTTGGGC CACCCCAGCA GGGTCTGGAA
    CTTGGTGTCG GTGATGGTGT TCTCCTCAAG GCGGTGGCCC AGGCCACAGG GCGGCAGCTG
    GACTCTGTTA AGGCAGAGGC AGCTGAGAAG GGTGACGTGG GGCTGGTGGC CGAAAACAGC
    CGCAGTACCC AGAGGCTCAT GCTGCCCCCA CCTGCTCTCA CCGCTGCCGG GGTCTTCGCC
    AAGTTCCGAG ACATTGCCCG GCTTGCTGGC AGTGCCTCCA CAGCCAAGAA GATAGATGTC
    ATCAAAGGCC TCTTCGTCGC CTGCCGCCAC TCAGAAGCCC GATTCATTGC CAGGGCCCTG
    AGTGGACGGC TGCGCCTCGG GCTGGCAGAG CAGTCGGTGC TGGCTGCCCT CGCCCAGGCC
    GTGAGCCTCA CGCCCCCAGG CCAAGAATTC CCCCCGGCCG TTGTGGATGC TGGGAAGGGC
    CGGACAGCAG AAGCCCGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACG
    TTCTGCGAGG TGCCCGACCT GGACCGGATC ATCCCTGTAC TGCTGGAGCA TGGCCTGGAG
    CACCTCCCTG AGCACTGCAG GTTGAGCCCA GGGGTTCCAC TGAAACCAAT GCTGGCGCAC
    CCCACCCGGG GTGTCAGCGA GGTCCTAAAA CGTTTTGAAG AGGCAGCTTT CACCTGCGAG
    TACAAATACG ATGGGCAGAG GGCACAGATC CACGTTCTGG AAGGTGGGGA GGTGAAGATC
    TTCAGCAGGA ATCAGGAAGA CAATACCGGA AAGTACCCAG ACATCATCAG CCGCATTCCC
    AAGATTAAAC TCCCGTCAGT CACGTCTTTC ATTCTGGACA CAGAAGCTGT GGCATGGGAC
    CGAGAAAAGA AGCAGATCCA GCCATTCCAA GTGCTCACCA CCCGCAAACG CAAGGAGGTG
    GATGCAGCAG AGATCCAGGT GCAGGTGTGT CTATATGCCT TCGACCTCAT TTACCTCAAT
    GGAGAGTCCC TGGTACGTGA GCCTCTTTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTT
    GTGGAGACGG AGGGCGAGTT TGTGTTCGCC ACCTCCCTGG ACACCAAGGA CACGGACCAA
    ATTGCCGAGT TCTTAGAGCA GTCTGTGAAA GACTCCTGTG AGGGGCTGAT GGTGAAAACT
    CTGGACGTTG ATGCCACCTA TGAGATCGCC AAGAGATCCC ACAACTGGCT CAAGCTGAAG
    AAGGACTACC TCGATGGTGT GGGCGACACC CTGGACCTCG TGGTGATTGG CGCCTACCTG
    GGCCGTGGCA AACGGGCTGG CCGCTATGGG GGCTTCCTAC TGGCTGCCTA CGACGAGGAG
    AGTGAGGAGC TCCAGGCCAT ATGCAAGCTC GGAACTGGCT TCAGTGACGA GGAACTGGAG
    GAGCACTACC AGAGGCTCCA GGCCCTGGTG CTGCCCACCC CACGCTCCTA TGTCCGGGTC
    GACGGTGCCG TGGCCCCCGA TCACTGGCTG GACCCCAGTG CCGTGTGGGA GGTGAAGTGT
    GCGGATCTGT CCTTGTCGCC CATCTACCCT GCTGCCCGGG GCCTGGTGGA TGGTGAGAAG
    GGGATCTCCC TTCGCTTTCC TCGGTTTATC CGGGTCCGTG AAGACAAGAA GCCAGAGGAG
    GCCACCACCA GTGGCCAGGT GGCCTGCCTG TACATGAAGC AGAGTCAGAT TCAGAACCAG
    CAGGGCGCGG ACTTAGACTC TGACTCAGAT TTCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616431.1
    CDS173..2911
    Translation

    Target ORF information:

    RefSeq Version XM_005616374.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ligase I, DNA, ATP-dependent (LIG1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616374.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGCAGAGAC CCCACCAGAA
    AGCCTGTCAG AATCTGAAGT GACCCAAGAG AAGGAGGCAG AAGGGGAGGA CCAACCTGTG
    ACATCCCCTG AGCACCGAGA GACCTCCCAG CCCCCGGTGG ATAGCACGTT GGAGCCTGCG
    GTGTCTGGGA AGCAGGAGGC TCAGAACGAG GACTTGGCTA AGCCGCCCAC CAGAGCCCCC
    AAGACGGTCA GCAGCTTCTT TGCCCCGCGG AAACCGGCAA CCAAGAAAGA AGGGAAAGAA
    GAGGACCCTG GCACTCCAAG GCAGGAGGAG ACCAAGGGAC CCCTGGACCC TCCCAGCTAC
    AATCCTGCCA AGAACAACTA CCATCCCATT GATGATGCAT GCTGGAAGCC AGGCCAGAAG
    GTCCCTTACC TGGCTGTGGC CCGGACATTT GAGAAGATTG AGGAAGTTTC TGCTCGGCTC
    CGGATGGTGG AGACGCTGAG CAACTTGCTG CGCTCTGTGG TGGCCCTGTC ACCTCCGGAC
    CTCCTTCCTA TCCTCTACCT CAGCCTCAAC CGTCTTGGGC CACCCCAGCA GGGTCTGGAA
    CTTGGTGTCG GTGATGGTGT TCTCCTCAAG GCGGTGGCCC AGGCCACAGG GCGGCAGCTG
    GACTCTGTTA AGGCAGAGGC AGCTGAGAAG GGTGACGTGG GGCTGGTGGC CGAAAACAGC
    CGCAGTACCC AGAGGCTCAT GCTGCCCCCA CCTGCTCTCA CCGCTGCCGG GGTCTTCGCC
    AAGTTCCGAG ACATTGCCCG GCTTGCTGGC AGTGCCTCCA CAGCCAAGAA GATAGATGTC
    ATCAAAGGCC TCTTCGTCGC CTGCCGCCAC TCAGAAGCCC GATTCATTGC CAGGGCCCTG
    AGTGGACGGC TGCGCCTCGG GCTGGCAGAG CAGTCGGTGC TGGCTGCCCT CGCCCAGGCC
    GTGAGCCTCA CGCCCCCAGG CCAAGAATTC CCCCCGGCCG TTGTGGATGC TGGGAAGGGC
    CGGACAGCAG AAGCCCGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACG
    TTCTGCGAGG TGCCCGACCT GGACCGGATC ATCCCTGTAC TGCTGGAGCA TGGCCTGGAG
    CACCTCCCTG AGCACTGCAG GTTGAGCCCA GGGGTTCCAC TGAAACCAAT GCTGGCGCAC
    CCCACCCGGG GTGTCAGCGA GGTCCTAAAA CGTTTTGAAG AGGCAGCTTT CACCTGCGAG
    TACAAATACG ATGGGCAGAG GGCACAGATC CACGTTCTGG AAGGTGGGGA GGTGAAGATC
    TTCAGCAGGA ATCAGGAAGA CAATACCGGA AAGTACCCAG ACATCATCAG CCGCATTCCC
    AAGATTAAAC TCCCGTCAGT CACGTCTTTC ATTCTGGACA CAGAAGCTGT GGCATGGGAC
    CGAGAAAAGA AGCAGATCCA GCCATTCCAA GTGCTCACCA CCCGCAAACG CAAGGAGGTG
    GATGCAGCAG AGATCCAGGT GCAGGTGTGT CTATATGCCT TCGACCTCAT TTACCTCAAT
    GGAGAGTCCC TGGTACGTGA GCCTCTTTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTT
    GTGGAGACGG AGGGCGAGTT TGTGTTCGCC ACCTCCCTGG ACACCAAGGA CACGGACCAA
    ATTGCCGAGT TCTTAGAGCA GTCTGTGAAA GACTCCTGTG AGGGGCTGAT GGTGAAAACT
    CTGGACGTTG ATGCCACCTA TGAGATCGCC AAGAGATCCC ACAACTGGCT CAAGCTGAAG
    AAGGACTACC TCGATGGTGT GGGCGACACC CTGGACCTCG TGGTGATTGG CGCCTACCTG
    GGCCGTGGCA AACGGGCTGG CCGCTATGGG GGCTTCCTAC TGGCTGCCTA CGACGAGGAG
    AGTGAGGAGC TCCAGGCCAT ATGCAAGCTC GGAACTGGCT TCAGTGACGA GGAACTGGAG
    GAGCACTACC AGAGGCTCCA GGCCCTGGTG CTGCCCACCC CACGCTCCTA TGTCCGGGTC
    GACGGTGCCG TGGCCCCCGA TCACTGGCTG GACCCCAGTG CCGTGTGGGA GGTGAAGTGT
    GCGGATCTGT CCTTGTCGCC CATCTACCCT GCTGCCCGGG GCCTGGTGGA TGGTGAGAAG
    GGGATCTCCC TTCGCTTTCC TCGGTTTATC CGGGTCCGTG AAGACAAGAA GCCAGAGGAG
    GCCACCACCA GTGGCCAGGT GGCCTGCCTG TACATGAAGC AGAGTCAGAT TCAGAACCAG
    CAGGGCGCGG ACTTAGACTC TGACTCAGAT TTCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01933
    Clone ID Related Accession (Same CDS sequence) XM_005616375.3 , XM_005616375.2
    Accession Version XM_005616375.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA ligase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005616375.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGAGACCCC ACCAGAAAGC
    CTGTCAGAAT CTGAAGTGAC CCAAGAGAAG GAGGCAGAAG GGGAGGACCA ACCTGTGACA
    TCCCCTGAGC ACCGAGAGAC CTCCCCAGAG CCCCCGGTGG ATAGCACGTT GGAGCCTGCG
    GTGTCTGGGA AGCAGGAGGC TCAGAACGAG GACTTGGCTA AGCCGCCCAC CAGAGCCCCC
    AAGACGGTCA GCAGCTTCTT TGCCCCGCGG AAACCGGCAA CCAAGAAAGA AGGGAAAGAA
    GAGGACCCTG GCACTCCAAG GCAGGAGGAG ACCAAGGGAC CCCTGGACCC TCCCAGCTAC
    AATCCTGCCA AGAACAACTA CCATCCCATT GATGATGCAT GCTGGAAGCC AGGCCAGAAG
    GTCCCTTACC TGGCTGTGGC CCGGACATTT GAGAAGATTG AGGAAGTTTC TGCTCGGCTC
    CGGATGGTGG AGACGCTGAG CAACTTGCTG CGCTCTGTGG TGGCCCTGTC ACCTCCGGAC
    CTCCTTCCTA TCCTCTACCT CAGCCTCAAC CGTCTTGGGC CACCCCAGCA GGGTCTGGAA
    CTTGGTGTCG GTGATGGTGT TCTCCTCAAG GCGGTGGCCC AGGCCACAGG GCGGCAGCTG
    GACTCTGTTA AGGCAGAGGC AGCTGAGAAG GGTGACGTGG GGCTGGTGGC CGAAAACAGC
    CGCAGTACCC AGAGGCTCAT GCTGCCCCCA CCTGCTCTCA CCGCTGCCGG GGTCTTCGCC
    AAGTTCCGAG ACATTGCCCG GCTTGCTGGC AGTGCCTCCA CAGCCAAGAA GATAGATGTC
    ATCAAAGGCC TCTTCGTCGC CTGCCGCCAC TCAGAAGCCC GATTCATTGC CAGGGCCCTG
    AGTGGACGGC TGCGCCTCGG GCTGGCAGAG CAGTCGGTGC TGGCTGCCCT CGCCCAGGCC
    GTGAGCCTCA CGCCCCCAGG CCAAGAATTC CCCCCGGCCG TTGTGGATGC TGGGAAGGGC
    CGGACAGCAG AAGCCCGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACG
    TTCTGCGAGG TGCCCGACCT GGACCGGATC ATCCCTGTAC TGCTGGAGCA TGGCCTGGAG
    CACCTCCCTG AGCACTGCAG GTTGAGCCCA GGGGTTCCAC TGAAACCAAT GCTGGCGCAC
    CCCACCCGGG GTGTCAGCGA GGTCCTAAAA CGTTTTGAAG AGGCAGCTTT CACCTGCGAG
    TACAAATACG ATGGGCAGAG GGCACAGATC CACGTTCTGG AAGGTGGGGA GGTGAAGATC
    TTCAGCAGGA ATCAGGAAGA CAATACCGGA AAGTACCCAG ACATCATCAG CCGCATTCCC
    AAGATTAAAC TCCCGTCAGT CACGTCTTTC ATTCTGGACA CAGAAGCTGT GGCATGGGAC
    CGAGAAAAGA AGCAGATCCA GCCATTCCAA GTGCTCACCA CCCGCAAACG CAAGGAGGTG
    GATGCAGCAG AGATCCAGGT GCAGGTGTGT CTATATGCCT TCGACCTCAT TTACCTCAAT
    GGAGAGTCCC TGGTACGTGA GCCTCTTTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTT
    GTGGAGACGG AGGGCGAGTT TGTGTTCGCC ACCTCCCTGG ACACCAAGGA CACGGACCAA
    ATTGCCGAGT TCTTAGAGCA GTCTGTGAAA GACTCCTGTG AGGGGCTGAT GGTGAAAACT
    CTGGACGTTG ATGCCACCTA TGAGATCGCC AAGAGATCCC ACAACTGGCT CAAGCTGAAG
    AAGGACTACC TCGATGGTGT GGGCGACACC CTGGACCTCG TGGTGATTGG CGCCTACCTG
    GGCCGTGGCA AACGGGCTGG CCGCTATGGG GGCTTCCTAC TGGCTGCCTA CGACGAGGAG
    AGTGAGGAGC TCCAGGCCAT ATGCAAGCTC GGAACTGGCT TCAGTGACGA GGAACTGGAG
    GAGCACTACC AGAGGCTCCA GGCCCTGGTG CTGCCCACCC CACGCTCCTA TGTCCGGGTC
    GACGGTGCCG TGGCCCCCGA TCACTGGCTG GACCCCAGTG CCGTGTGGGA GGTGAAGTGT
    GCGGATCTGT CCTTGTCGCC CATCTACCCT GCTGCCCGGG GCCTGGTGGA TGGTGAGAAG
    GGGATCTCCC TTCGCTTTCC TCGGTTTATC CGGGTCCGTG AAGACAAGAA GCCAGAGGAG
    GCCACCACCA GTGGCCAGGT GGCCTGCCTG TACATGAAGC AGAGTCAGAT TCAGAACCAG
    CAGGGCGCGG ACTTAGACTC TGACTCAGAT TTCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616432.1
    CDS194..2932
    Translation

    Target ORF information:

    RefSeq Version XM_005616375.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA ligase 1 (LIG1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616375.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGAGACCCC ACCAGAAAGC
    CTGTCAGAAT CTGAAGTGAC CCAAGAGAAG GAGGCAGAAG GGGAGGACCA ACCTGTGACA
    TCCCCTGAGC ACCGAGAGAC CTCCCCAGAG CCCCCGGTGG ATAGCACGTT GGAGCCTGCG
    GTGTCTGGGA AGCAGGAGGC TCAGAACGAG GACTTGGCTA AGCCGCCCAC CAGAGCCCCC
    AAGACGGTCA GCAGCTTCTT TGCCCCGCGG AAACCGGCAA CCAAGAAAGA AGGGAAAGAA
    GAGGACCCTG GCACTCCAAG GCAGGAGGAG ACCAAGGGAC CCCTGGACCC TCCCAGCTAC
    AATCCTGCCA AGAACAACTA CCATCCCATT GATGATGCAT GCTGGAAGCC AGGCCAGAAG
    GTCCCTTACC TGGCTGTGGC CCGGACATTT GAGAAGATTG AGGAAGTTTC TGCTCGGCTC
    CGGATGGTGG AGACGCTGAG CAACTTGCTG CGCTCTGTGG TGGCCCTGTC ACCTCCGGAC
    CTCCTTCCTA TCCTCTACCT CAGCCTCAAC CGTCTTGGGC CACCCCAGCA GGGTCTGGAA
    CTTGGTGTCG GTGATGGTGT TCTCCTCAAG GCGGTGGCCC AGGCCACAGG GCGGCAGCTG
    GACTCTGTTA AGGCAGAGGC AGCTGAGAAG GGTGACGTGG GGCTGGTGGC CGAAAACAGC
    CGCAGTACCC AGAGGCTCAT GCTGCCCCCA CCTGCTCTCA CCGCTGCCGG GGTCTTCGCC
    AAGTTCCGAG ACATTGCCCG GCTTGCTGGC AGTGCCTCCA CAGCCAAGAA GATAGATGTC
    ATCAAAGGCC TCTTCGTCGC CTGCCGCCAC TCAGAAGCCC GATTCATTGC CAGGGCCCTG
    AGTGGACGGC TGCGCCTCGG GCTGGCAGAG CAGTCGGTGC TGGCTGCCCT CGCCCAGGCC
    GTGAGCCTCA CGCCCCCAGG CCAAGAATTC CCCCCGGCCG TTGTGGATGC TGGGAAGGGC
    CGGACAGCAG AAGCCCGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACG
    TTCTGCGAGG TGCCCGACCT GGACCGGATC ATCCCTGTAC TGCTGGAGCA TGGCCTGGAG
    CACCTCCCTG AGCACTGCAG GTTGAGCCCA GGGGTTCCAC TGAAACCAAT GCTGGCGCAC
    CCCACCCGGG GTGTCAGCGA GGTCCTAAAA CGTTTTGAAG AGGCAGCTTT CACCTGCGAG
    TACAAATACG ATGGGCAGAG GGCACAGATC CACGTTCTGG AAGGTGGGGA GGTGAAGATC
    TTCAGCAGGA ATCAGGAAGA CAATACCGGA AAGTACCCAG ACATCATCAG CCGCATTCCC
    AAGATTAAAC TCCCGTCAGT CACGTCTTTC ATTCTGGACA CAGAAGCTGT GGCATGGGAC
    CGAGAAAAGA AGCAGATCCA GCCATTCCAA GTGCTCACCA CCCGCAAACG CAAGGAGGTG
    GATGCAGCAG AGATCCAGGT GCAGGTGTGT CTATATGCCT TCGACCTCAT TTACCTCAAT
    GGAGAGTCCC TGGTACGTGA GCCTCTTTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTT
    GTGGAGACGG AGGGCGAGTT TGTGTTCGCC ACCTCCCTGG ACACCAAGGA CACGGACCAA
    ATTGCCGAGT TCTTAGAGCA GTCTGTGAAA GACTCCTGTG AGGGGCTGAT GGTGAAAACT
    CTGGACGTTG ATGCCACCTA TGAGATCGCC AAGAGATCCC ACAACTGGCT CAAGCTGAAG
    AAGGACTACC TCGATGGTGT GGGCGACACC CTGGACCTCG TGGTGATTGG CGCCTACCTG
    GGCCGTGGCA AACGGGCTGG CCGCTATGGG GGCTTCCTAC TGGCTGCCTA CGACGAGGAG
    AGTGAGGAGC TCCAGGCCAT ATGCAAGCTC GGAACTGGCT TCAGTGACGA GGAACTGGAG
    GAGCACTACC AGAGGCTCCA GGCCCTGGTG CTGCCCACCC CACGCTCCTA TGTCCGGGTC
    GACGGTGCCG TGGCCCCCGA TCACTGGCTG GACCCCAGTG CCGTGTGGGA GGTGAAGTGT
    GCGGATCTGT CCTTGTCGCC CATCTACCCT GCTGCCCGGG GCCTGGTGGA TGGTGAGAAG
    GGGATCTCCC TTCGCTTTCC TCGGTTTATC CGGGTCCGTG AAGACAAGAA GCCAGAGGAG
    GCCACCACCA GTGGCCAGGT GGCCTGCCTG TACATGAAGC AGAGTCAGAT TCAGAACCAG
    CAGGGCGCGG ACTTAGACTC TGACTCAGAT TTCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01933
    Clone ID Related Accession (Same CDS sequence) XM_005616375.3 , XM_005616375.2
    Accession Version XM_005616375.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DNA ligase 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005616375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGAGACCCC ACCAGAAAGC
    CTGTCAGAAT CTGAAGTGAC CCAAGAGAAG GAGGCAGAAG GGGAGGACCA ACCTGTGACA
    TCCCCTGAGC ACCGAGAGAC CTCCCCAGAG CCCCCGGTGG ATAGCACGTT GGAGCCTGCG
    GTGTCTGGGA AGCAGGAGGC TCAGAACGAG GACTTGGCTA AGCCGCCCAC CAGAGCCCCC
    AAGACGGTCA GCAGCTTCTT TGCCCCGCGG AAACCGGCAA CCAAGAAAGA AGGGAAAGAA
    GAGGACCCTG GCACTCCAAG GCAGGAGGAG ACCAAGGGAC CCCTGGACCC TCCCAGCTAC
    AATCCTGCCA AGAACAACTA CCATCCCATT GATGATGCAT GCTGGAAGCC AGGCCAGAAG
    GTCCCTTACC TGGCTGTGGC CCGGACATTT GAGAAGATTG AGGAAGTTTC TGCTCGGCTC
    CGGATGGTGG AGACGCTGAG CAACTTGCTG CGCTCTGTGG TGGCCCTGTC ACCTCCGGAC
    CTCCTTCCTA TCCTCTACCT CAGCCTCAAC CGTCTTGGGC CACCCCAGCA GGGTCTGGAA
    CTTGGTGTCG GTGATGGTGT TCTCCTCAAG GCGGTGGCCC AGGCCACAGG GCGGCAGCTG
    GACTCTGTTA AGGCAGAGGC AGCTGAGAAG GGTGACGTGG GGCTGGTGGC CGAAAACAGC
    CGCAGTACCC AGAGGCTCAT GCTGCCCCCA CCTGCTCTCA CCGCTGCCGG GGTCTTCGCC
    AAGTTCCGAG ACATTGCCCG GCTTGCTGGC AGTGCCTCCA CAGCCAAGAA GATAGATGTC
    ATCAAAGGCC TCTTCGTCGC CTGCCGCCAC TCAGAAGCCC GATTCATTGC CAGGGCCCTG
    AGTGGACGGC TGCGCCTCGG GCTGGCAGAG CAGTCGGTGC TGGCTGCCCT CGCCCAGGCC
    GTGAGCCTCA CGCCCCCAGG CCAAGAATTC CCCCCGGCCG TTGTGGATGC TGGGAAGGGC
    CGGACAGCAG AAGCCCGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACG
    TTCTGCGAGG TGCCCGACCT GGACCGGATC ATCCCTGTAC TGCTGGAGCA TGGCCTGGAG
    CACCTCCCTG AGCACTGCAG GTTGAGCCCA GGGGTTCCAC TGAAACCAAT GCTGGCGCAC
    CCCACCCGGG GTGTCAGCGA GGTCCTAAAA CGTTTTGAAG AGGCAGCTTT CACCTGCGAG
    TACAAATACG ATGGGCAGAG GGCACAGATC CACGTTCTGG AAGGTGGGGA GGTGAAGATC
    TTCAGCAGGA ATCAGGAAGA CAATACCGGA AAGTACCCAG ACATCATCAG CCGCATTCCC
    AAGATTAAAC TCCCGTCAGT CACGTCTTTC ATTCTGGACA CAGAAGCTGT GGCATGGGAC
    CGAGAAAAGA AGCAGATCCA GCCATTCCAA GTGCTCACCA CCCGCAAACG CAAGGAGGTG
    GATGCAGCAG AGATCCAGGT GCAGGTGTGT CTATATGCCT TCGACCTCAT TTACCTCAAT
    GGAGAGTCCC TGGTACGTGA GCCTCTTTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTT
    GTGGAGACGG AGGGCGAGTT TGTGTTCGCC ACCTCCCTGG ACACCAAGGA CACGGACCAA
    ATTGCCGAGT TCTTAGAGCA GTCTGTGAAA GACTCCTGTG AGGGGCTGAT GGTGAAAACT
    CTGGACGTTG ATGCCACCTA TGAGATCGCC AAGAGATCCC ACAACTGGCT CAAGCTGAAG
    AAGGACTACC TCGATGGTGT GGGCGACACC CTGGACCTCG TGGTGATTGG CGCCTACCTG
    GGCCGTGGCA AACGGGCTGG CCGCTATGGG GGCTTCCTAC TGGCTGCCTA CGACGAGGAG
    AGTGAGGAGC TCCAGGCCAT ATGCAAGCTC GGAACTGGCT TCAGTGACGA GGAACTGGAG
    GAGCACTACC AGAGGCTCCA GGCCCTGGTG CTGCCCACCC CACGCTCCTA TGTCCGGGTC
    GACGGTGCCG TGGCCCCCGA TCACTGGCTG GACCCCAGTG CCGTGTGGGA GGTGAAGTGT
    GCGGATCTGT CCTTGTCGCC CATCTACCCT GCTGCCCGGG GCCTGGTGGA TGGTGAGAAG
    GGGATCTCCC TTCGCTTTCC TCGGTTTATC CGGGTCCGTG AAGACAAGAA GCCAGAGGAG
    GCCACCACCA GTGGCCAGGT GGCCTGCCTG TACATGAAGC AGAGTCAGAT TCAGAACCAG
    CAGGGCGCGG ACTTAGACTC TGACTCAGAT TTCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616432.1
    CDS174..2912
    Translation

    Target ORF information:

    RefSeq Version XM_005616375.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ligase I, DNA, ATP-dependent (LIG1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616375.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGAGACCCC ACCAGAAAGC
    CTGTCAGAAT CTGAAGTGAC CCAAGAGAAG GAGGCAGAAG GGGAGGACCA ACCTGTGACA
    TCCCCTGAGC ACCGAGAGAC CTCCCCAGAG CCCCCGGTGG ATAGCACGTT GGAGCCTGCG
    GTGTCTGGGA AGCAGGAGGC TCAGAACGAG GACTTGGCTA AGCCGCCCAC CAGAGCCCCC
    AAGACGGTCA GCAGCTTCTT TGCCCCGCGG AAACCGGCAA CCAAGAAAGA AGGGAAAGAA
    GAGGACCCTG GCACTCCAAG GCAGGAGGAG ACCAAGGGAC CCCTGGACCC TCCCAGCTAC
    AATCCTGCCA AGAACAACTA CCATCCCATT GATGATGCAT GCTGGAAGCC AGGCCAGAAG
    GTCCCTTACC TGGCTGTGGC CCGGACATTT GAGAAGATTG AGGAAGTTTC TGCTCGGCTC
    CGGATGGTGG AGACGCTGAG CAACTTGCTG CGCTCTGTGG TGGCCCTGTC ACCTCCGGAC
    CTCCTTCCTA TCCTCTACCT CAGCCTCAAC CGTCTTGGGC CACCCCAGCA GGGTCTGGAA
    CTTGGTGTCG GTGATGGTGT TCTCCTCAAG GCGGTGGCCC AGGCCACAGG GCGGCAGCTG
    GACTCTGTTA AGGCAGAGGC AGCTGAGAAG GGTGACGTGG GGCTGGTGGC CGAAAACAGC
    CGCAGTACCC AGAGGCTCAT GCTGCCCCCA CCTGCTCTCA CCGCTGCCGG GGTCTTCGCC
    AAGTTCCGAG ACATTGCCCG GCTTGCTGGC AGTGCCTCCA CAGCCAAGAA GATAGATGTC
    ATCAAAGGCC TCTTCGTCGC CTGCCGCCAC TCAGAAGCCC GATTCATTGC CAGGGCCCTG
    AGTGGACGGC TGCGCCTCGG GCTGGCAGAG CAGTCGGTGC TGGCTGCCCT CGCCCAGGCC
    GTGAGCCTCA CGCCCCCAGG CCAAGAATTC CCCCCGGCCG TTGTGGATGC TGGGAAGGGC
    CGGACAGCAG AAGCCCGAAA GACGTGGCTG GAGGAGCAAG GCATGATCCT GAAGCAGACG
    TTCTGCGAGG TGCCCGACCT GGACCGGATC ATCCCTGTAC TGCTGGAGCA TGGCCTGGAG
    CACCTCCCTG AGCACTGCAG GTTGAGCCCA GGGGTTCCAC TGAAACCAAT GCTGGCGCAC
    CCCACCCGGG GTGTCAGCGA GGTCCTAAAA CGTTTTGAAG AGGCAGCTTT CACCTGCGAG
    TACAAATACG ATGGGCAGAG GGCACAGATC CACGTTCTGG AAGGTGGGGA GGTGAAGATC
    TTCAGCAGGA ATCAGGAAGA CAATACCGGA AAGTACCCAG ACATCATCAG CCGCATTCCC
    AAGATTAAAC TCCCGTCAGT CACGTCTTTC ATTCTGGACA CAGAAGCTGT GGCATGGGAC
    CGAGAAAAGA AGCAGATCCA GCCATTCCAA GTGCTCACCA CCCGCAAACG CAAGGAGGTG
    GATGCAGCAG AGATCCAGGT GCAGGTGTGT CTATATGCCT TCGACCTCAT TTACCTCAAT
    GGAGAGTCCC TGGTACGTGA GCCTCTTTCC CGACGCCGGC AGCTGCTCCG GGAGAACTTT
    GTGGAGACGG AGGGCGAGTT TGTGTTCGCC ACCTCCCTGG ACACCAAGGA CACGGACCAA
    ATTGCCGAGT TCTTAGAGCA GTCTGTGAAA GACTCCTGTG AGGGGCTGAT GGTGAAAACT
    CTGGACGTTG ATGCCACCTA TGAGATCGCC AAGAGATCCC ACAACTGGCT CAAGCTGAAG
    AAGGACTACC TCGATGGTGT GGGCGACACC CTGGACCTCG TGGTGATTGG CGCCTACCTG
    GGCCGTGGCA AACGGGCTGG CCGCTATGGG GGCTTCCTAC TGGCTGCCTA CGACGAGGAG
    AGTGAGGAGC TCCAGGCCAT ATGCAAGCTC GGAACTGGCT TCAGTGACGA GGAACTGGAG
    GAGCACTACC AGAGGCTCCA GGCCCTGGTG CTGCCCACCC CACGCTCCTA TGTCCGGGTC
    GACGGTGCCG TGGCCCCCGA TCACTGGCTG GACCCCAGTG CCGTGTGGGA GGTGAAGTGT
    GCGGATCTGT CCTTGTCGCC CATCTACCCT GCTGCCCGGG GCCTGGTGGA TGGTGAGAAG
    GGGATCTCCC TTCGCTTTCC TCGGTTTATC CGGGTCCGTG AAGACAAGAA GCCAGAGGAG
    GCCACCACCA GTGGCCAGGT GGCCTGCCTG TACATGAAGC AGAGTCAGAT TCAGAACCAG
    CAGGGCGCGG ACTTAGACTC TGACTCAGAT TTCTACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286225
    Clone ID Related Accession (Same CDS sequence) XM_022424455.1
    Accession Version XM_022424455.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product DNA ligase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGAGACCCC ACCAGAAAGC
    CTGTCAGAAT CTGAAGTGAC CCAAGAGAAG GAGGCAGAAG GGGAGGACCA ACCTGTGACA
    TCCCCTGAGC ACCGAGAGAC CTCCCAGCCC CCGGTGGATA GCACGTTGGA GCCTGCGGTG
    TCTGGGAAGC AGGAGGCTCA GAACGAGGAC TTGGCTAAGC CGCCCACCAG AGCCCCCAAG
    ACGGTCAGCA GCTTCTTTGC CCCGCGGAAA CCGGCAACCA AGAAAGAAGG GAAAGAAGAG
    GACCCTGGCA CTCCAAGGCA GGAGGAGACC AAGGGACCCC TGGACCCTCC CAGCTACAAT
    CCTGCCAAGA ACAACTACCA TCCCATTGAT GATGCATGCT GGAAGCCAGG CCAGAAGGTC
    CCTTACCTGG CTGTGGCCCG GACATTTGAG AAGATTGAGG AAGTTTCTGC TCGGCTCCGG
    ATGGTGGAGA CGCTGAGCAA CTTGCTGCGC TCTGTGGTGG CCCTGTCACC TCCGGACCTC
    CTTCCTATCC TCTACCTCAG CCTCAACCGT CTTGGGCCAC CCCAGCAGGG TCTGGAACTT
    GGTGTCGGTG ATGGTGTTCT CCTCAAGGCG GTGGCCCAGG CCACAGGGCG GCAGCTGGAC
    TCTGTTAAGG CAGAGGCAGC TGAGAAGGGT GACGTGGGGC TGGTGGCCGA AAACAGCCGC
    AGTACCCAGA GGCTCATGCT GCCCCCACCT GCTCTCACCG CTGCCGGGGT CTTCGCCAAG
    TTCCGAGACA TTGCCCGGCT TGCTGGCAGT GCCTCCACAG CCAAGAAGAT AGATGTCATC
    AAAGGCCTCT TCGTCGCCTG CCGCCACTCA GAAGCCCGAT TCATTGCCAG GGCCCTGAGT
    GGACGGCTGC GCCTCGGGCT GGCAGAGCAG TCGGTGCTGG CTGCCCTCGC CCAGGCCGTG
    AGCCTCACGC CCCCAGGCCA AGAATTCCCC CCGGCCGTTG TGGATGCTGG GAAGGGCCGG
    ACAGCAGAAG CCCGAAAGAC GTGGCTGGAG GAGCAAGGCA TGATCCTGAA GCAGACGTTC
    TGCGAGGTGC CCGACCTGGA CCGGATCATC CCTGTACTGC TGGAGCATGG CCTGGAGCAC
    CTCCCTGAGC ACTGCAGGTT GAGCCCAGGG GTTCCACTGA AACCAATGCT GGCGCACCCC
    ACCCGGGGTG TCAGCGAGGT CCTAAAACGT TTTGAAGAGG CAGCTTTCAC CTGCGAGTAC
    AAATACGATG GGCAGAGGGC ACAGATCCAC GTTCTGGAAG GTGGGGAGGT GAAGATCTTC
    AGCAGGAATC AGGAAGACAA TACCGGAAAG TACCCAGACA TCATCAGCCG CATTCCCAAG
    ATTAAACTCC CGTCAGTCAC GTCTTTCATT CTGGACACAG AAGCTGTGGC ATGGGACCGA
    GAAAAGAAGC AGATCCAGCC ATTCCAAGTG CTCACCACCC GCAAACGCAA GGAGGTGGAT
    GCAGCAGAGA TCCAGGTGCA GGTGTGTCTA TATGCCTTCG ACCTCATTTA CCTCAATGGA
    GAGTCCCTGG TACGTGAGCC TCTTTCCCGA CGCCGGCAGC TGCTCCGGGA GAACTTTGTG
    GAGACGGAGG GCGAGTTTGT GTTCGCCACC TCCCTGGACA CCAAGGACAC GGACCAAATT
    GCCGAGTTCT TAGAGCAGTC TGTGAAAGAC TCCTGTGAGG GGCTGATGGT GAAAACTCTG
    GACGTTGATG CCACCTATGA GATCGCCAAG AGATCCCACA ACTGGCTCAA GCTGAAGAAG
    GACTACCTCG ATGGTGTGGG CGACACCCTG GACCTCGTGG TGATTGGCGC CTACCTGGGC
    CGTGGCAAAC GGGCTGGCCG CTATGGGGGC TTCCTACTGG CTGCCTACGA CGAGGAGAGT
    GAGGAGCTCC AGGCCATATG CAAGCTCGGA ACTGGCTTCA GTGACGAGGA ACTGGAGGAG
    CACTACCAGA GGCTCCAGGC CCTGGTGCTG CCCACCCCAC GCTCCTATGT CCGGGTCGAC
    GGTGCCGTGG CCCCCGATCA CTGGCTGGAC CCCAGTGCCG TGTGGGAGGT GAAGTGTGCG
    GATCTGTCCT TGTCGCCCAT CTACCCTGCT GCCCGGGGCC TGGTGGATGG TGAGAAGGGG
    ATCTCCCTTC GCTTTCCTCG GTTTATCCGG GTCCGTGAAG ACAAGAAGCC AGAGGAGGCC
    ACCACCAGTG GCCAGGTGGC CTGCCTGTAC ATGAAGCAGA GTCAGATTCA GAACCAGCAG
    GGCGCGGACT TAGACTCTGA CTCAGATTTC TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280163.1
    CDS194..2929
    Translation

    Target ORF information:

    RefSeq Version XM_022424455.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DNA ligase 1 (LIG1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424455.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGGA GTATCATGTC ATTTTTCTAC CCCAAGAAGG AGGGCAAAGC GAAAAAGCCA 
    GAGAAGGAGA CCTCCAACAG CATCAGAGAG ACAGAGCCAC CCCCAAAGGT GGCACTGAAG
    GAGCGGAATG GAGAGGTGCC TGAAAGCGAT TCTCCAGTGA AGAGGCCAGG GCGGAAGGTG
    GCCCGGGTCC TGGGCAGTGA AGGGGAGGAG GAGGACGAAG CCCTCACGCC ATCCAAAGGC
    CAGAAACCTG CCCCAGGCTC TCCACAAGTC TCCCCTCCCA GCCCTGTCAC ATATCCCGAG
    AGCAGCCCTT CCCTCTCCAG TACCTCTCCT GTGGACATCT CAGCAGGGAT CCCAAAGCGG
    CGCACTGCTC GGAAGCAGCT TCCTAAGCGG ACAATACAGG ATGTCCTGGA AGAGCAGAGC
    AAGGATGAGG ACAGAGAGGC CAAGAAGAAG AAGGAAGAAG AAGAGACCCC ACCAGAAAGC
    CTGTCAGAAT CTGAAGTGAC CCAAGAGAAG GAGGCAGAAG GGGAGGACCA ACCTGTGACA
    TCCCCTGAGC ACCGAGAGAC CTCCCAGCCC CCGGTGGATA GCACGTTGGA GCCTGCGGTG
    TCTGGGAAGC AGGAGGCTCA GAACGAGGAC TTGGCTAAGC CGCCCACCAG AGCCCCCAAG
    ACGGTCAGCA GCTTCTTTGC CCCGCGGAAA CCGGCAACCA AGAAAGAAGG GAAAGAAGAG
    GACCCTGGCA CTCCAAGGCA GGAGGAGACC AAGGGACCCC TGGACCCTCC CAGCTACAAT
    CCTGCCAAGA ACAACTACCA TCCCATTGAT GATGCATGCT GGAAGCCAGG CCAGAAGGTC
    CCTTACCTGG CTGTGGCCCG GACATTTGAG AAGATTGAGG AAGTTTCTGC TCGGCTCCGG
    ATGGTGGAGA CGCTGAGCAA CTTGCTGCGC TCTGTGGTGG CCCTGTCACC TCCGGACCTC
    CTTCCTATCC TCTACCTCAG CCTCAACCGT CTTGGGCCAC CCCAGCAGGG TCTGGAACTT
    GGTGTCGGTG ATGGTGTTCT CCTCAAGGCG GTGGCCCAGG CCACAGGGCG GCAGCTGGAC
    TCTGTTAAGG CAGAGGCAGC TGAGAAGGGT GACGTGGGGC TGGTGGCCGA AAACAGCCGC
    AGTACCCAGA GGCTCATGCT GCCCCCACCT GCTCTCACCG CTGCCGGGGT CTTCGCCAAG
    TTCCGAGACA TTGCCCGGCT TGCTGGCAGT GCCTCCACAG CCAAGAAGAT AGATGTCATC
    AAAGGCCTCT TCGTCGCCTG CCGCCACTCA GAAGCCCGAT TCATTGCCAG GGCCCTGAGT
    GGACGGCTGC GCCTCGGGCT GGCAGAGCAG TCGGTGCTGG CTGCCCTCGC CCAGGCCGTG
    AGCCTCACGC CCCCAGGCCA AGAATTCCCC CCGGCCGTTG TGGATGCTGG GAAGGGCCGG
    ACAGCAGAAG CCCGAAAGAC GTGGCTGGAG GAGCAAGGCA TGATCCTGAA GCAGACGTTC
    TGCGAGGTGC CCGACCTGGA CCGGATCATC CCTGTACTGC TGGAGCATGG CCTGGAGCAC
    CTCCCTGAGC ACTGCAGGTT GAGCCCAGGG GTTCCACTGA AACCAATGCT GGCGCACCCC
    ACCCGGGGTG TCAGCGAGGT CCTAAAACGT TTTGAAGAGG CAGCTTTCAC CTGCGAGTAC
    AAATACGATG GGCAGAGGGC ACAGATCCAC GTTCTGGAAG GTGGGGAGGT GAAGATCTTC
    AGCAGGAATC AGGAAGACAA TACCGGAAAG TACCCAGACA TCATCAGCCG CATTCCCAAG
    ATTAAACTCC CGTCAGTCAC GTCTTTCATT CTGGACACAG AAGCTGTGGC ATGGGACCGA
    GAAAAGAAGC AGATCCAGCC ATTCCAAGTG CTCACCACCC GCAAACGCAA GGAGGTGGAT
    GCAGCAGAGA TCCAGGTGCA GGTGTGTCTA TATGCCTTCG ACCTCATTTA CCTCAATGGA
    GAGTCCCTGG TACGTGAGCC TCTTTCCCGA CGCCGGCAGC TGCTCCGGGA GAACTTTGTG
    GAGACGGAGG GCGAGTTTGT GTTCGCCACC TCCCTGGACA CCAAGGACAC GGACCAAATT
    GCCGAGTTCT TAGAGCAGTC TGTGAAAGAC TCCTGTGAGG GGCTGATGGT GAAAACTCTG
    GACGTTGATG CCACCTATGA GATCGCCAAG AGATCCCACA ACTGGCTCAA GCTGAAGAAG
    GACTACCTCG ATGGTGTGGG CGACACCCTG GACCTCGTGG TGATTGGCGC CTACCTGGGC
    CGTGGCAAAC GGGCTGGCCG CTATGGGGGC TTCCTACTGG CTGCCTACGA CGAGGAGAGT
    GAGGAGCTCC AGGCCATATG CAAGCTCGGA ACTGGCTTCA GTGACGAGGA ACTGGAGGAG
    CACTACCAGA GGCTCCAGGC CCTGGTGCTG CCCACCCCAC GCTCCTATGT CCGGGTCGAC
    GGTGCCGTGG CCCCCGATCA CTGGCTGGAC CCCAGTGCCG TGTGGGAGGT GAAGTGTGCG
    GATCTGTCCT TGTCGCCCAT CTACCCTGCT GCCCGGGGCC TGGTGGATGG TGAGAAGGGG
    ATCTCCCTTC GCTTTCCTCG GTTTATCCGG GTCCGTGAAG ACAAGAAGCC AGAGGAGGCC
    ACCACCAGTG GCCAGGTGGC CTGCCTGTAC ATGAAGCAGA GTCAGATTCA GAACCAGCAG
    GGCGCGGACT TAGACTCTGA CTCAGATTTC TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.