BLM cDNA ORF clone, Canis lupus familiaris(dog)

The following BLM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BLM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe287231 XM_022416949.1
Latest version!
Canis lupus familiaris Bloom syndrome RecQ like helicase (BLM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe287232 XM_022416951.1
Latest version!
Canis lupus familiaris Bloom syndrome RecQ like helicase (BLM), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe287233 XM_022416952.1
Latest version!
Canis lupus familiaris Bloom syndrome RecQ like helicase (BLM), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe35734 XM_014112440.1
Latest version!
Canis lupus familiaris Bloom syndrome, RecQ helicase-like (BLM), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe35735 XM_014112441.1
Latest version!
Canis lupus familiaris Bloom syndrome, RecQ helicase-like (BLM), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe35736 XM_014112442.2
Latest version!
Canis lupus familiaris Bloom syndrome RecQ like helicase (BLM), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe04302 XM_003434379.4
Latest version!
Canis lupus familiaris Bloom syndrome RecQ like helicase (BLM), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe04302 XM_003434379.3 Canis lupus familiaris Bloom syndrome, RecQ helicase-like (BLM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe35736 XM_014112442.1 Canis lupus familiaris Bloom syndrome, RecQ helicase-like (BLM), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe287231
    Clone ID Related Accession (Same CDS sequence) XM_022416949.1
    Accession Version XM_022416949.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4272bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product Bloom syndrome protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGCCG TTCCCCAGAA TAATCTACAG GAGCAACTAG AACGCCATTC AGCCAGAAAA 
    CTTAGTAACA AATTAAGTCC TTCCAAACCG AAATCTTCAG TTTTCACTTT TAAGAAGAAA
    ACATCAGCCA ACAATGTGTC TGTCACTAGT GTGTCAGTAG CAAAAACACC TGTGTTAAAT
    GACAAAGATG TTAATGTCAC TGAGGCCTTT CCCTTCAGTG AACCTCTGCC CCACGCCACA
    AGTCAGCAGA CAAGGCCCAG TGACCTCAAA AATACTCCAG CAGGACAGCA AACCAGGAGA
    ACTGTTTCAA AACCATTATT GCCAGATTTG TCACTGGTTT CACGAGTTTT ATGTAATACC
    CAGAACACAC CGATTGTAAA GAAATCCAGT AATGCTACTT TCAAGAAATT GGAATTTAGC
    TCTTCATCAG ATTCTTTTGT TACCATCAAT GATTGGGATG ATATGGATGA CTTTGATACT
    TCTGGAAATT CAAAGCTTGT TACACCATGC AAAAATCACT TTGTGAGAGT TAGCACTGCT
    CAGAAGTCAA AAAAACCTAA GAGAAACTTT TTGAAAGCAC AGCTTCCTCA AGCAAATACA
    GTAAAGGCTG ATTTGACTCC ATCCTCCTTT GAAAGTAAGC AAGCATGTTT GACTAAAAAA
    CAGAACGACG ACTCGGAATG GTTTAGTAAT GATGTGATTT GCATTGATGA TGACCCCAAT
    CCTGAAGAGC TTATACATGG AGATCCTCAG GAGAGTCATT CTTTAAAAAC TCATTTGGAA
    GAGGAAAGAG ATAGCGAAAA GAAGAACTGG GAAGAAACAG AGTTATGTTC ATTGGAGAAG
    TCGCCATGTG TAGAGCTTGA CGAAGATGAT TACGATACAG ATTTTGTTCC ACCTTCTCCA
    GAAGTAATTT CTGCCTCTTC TTCCTCTTTA AAGTGTTTTA GTATGTTAAA GGACCTTGAT
    GCCTCTGGCA CAGAGGATGG TCCTAGCACA TCCGAGGACT TGCCAACACC CAAGGGGATG
    ACAACACAGC AGCCCAATCA AGAGACCAGC ACAAACTGTG ACGGTACAAA AACTAGACAG
    CCAAGTTTAC AGCAGCAGCT TATTCACGTG ATGGACCAGA TCTGTAAATT AGTAGATACT
    ATTCCTGATG ATGAACTGAA AGCTCTGACT TGTGGGAATG CACTGCATCA GCAGCGGGAC
    ATAAGAAGGA AGCTCCTAGC TGAAGCCGAC TTTAATACAA GTGATGCCAG TGTTCTTGCT
    TCGGTGTGGA CACGCGGGCC TGATTCACTG GGAAGCCCTG TGGAGGATAT GGCATCTCTT
    TCTGCTCCTG ATGTGTTTCC AAAGGGCAAT TCCCATCCTA CCGGGAATTC TGTTAAGGAG
    TTAAATTTTC CACACCTTCC CTCACATTCC AGTTCCACTG GGGAGTGTTT ACTGGCTACT
    ACCCCAGGAA AGACAGGATT CTCCGGCACC ATGAAGAATC CCTCTGAAAG ACTGTTATTC
    AGTCCCCATT TACAGAAGTC CTTTGTAAGT AGCAACTGGG CTGAAACACC ACGGATAGAA
    AAAAGGAACG AGAGCTCTTA CTTCCTGGGA AATGTTCTCA CAAGCACGGC TGTGAGAGAT
    CAGAATAAAC GTGCTGCTCC AATAAATGAC TTAGAAATTC AGGCTGCCTG TGATATTGAT
    AATTTTGACA TAGATGGCTT TGATGATGAT GATGACTGGG AAGATATAAT GCATGATTTA
    GCGGCCAGCA GATCTTCCAC AGCTGCCTGT CTACCTATTA AGGAAGGTCG GCCAGTTAAG
    CCAGTATCAG AAAGAATTTC TTCAGCCAAG ACAAACTGTC TTCCAGTGGC ATCTACTGCT
    CAAAATAAAA ACTCAGAGTC AATTCAGAAT TACACTGACA AGTCGGCGCA AAATTTAGCA
    TCCAGAAATC TGAAACATGA ACGTTTCCAA AGTCTTAGTT TTCCTCATAC AAAAGAAATG
    ATGAAGATTT TTCATAAAAA ATTTGGCCTG CATAATTTTA GAACTAATCA GCTAGAGGCG
    ATCAATGCTG CACTGCTGGG TGAAGACTGT TTTATCTTAA TGCCCACTGG AGGTGGTAAG
    AGTCTGTGTT ACCAGCTCCC TGCCTGTGTC TCTCCTGGTG TCACTATTGT CATTTCTCCT
    TTGAGATCAC TTATAGTAGA TCAAGTCCAA AAGCTGACTT CCTTGGATAT TCCAGCTACA
    TATCTGACAG GTGATAAGAC TGACTCGGAA GCAACAAGTA TTTACCTGCA ATTATCCAAG
    AAAGACCCAA TTATAAAACT TCTATATGTT ACTCCAGAGA AGGTCTGTGC AAGTAATAGA
    CTGCTTTCCA CTCTGGAGAA TCTGTATGAG AGGAAGCTCC TGGCACGTTT TGTCATTGAT
    GAAGCGCATT GTGTCAGTCA GTGGGGACAT GATTTTCGCC AAGATTATAA AAGAATGAAT
    ATGCTTCGCC AGAAGTTTCC CTCTGTTCCA GTGATGGCCC TTACTGCCAC AGCTAATCCC
    AGGGTACAGA AGGACATCCT CACTCAGCTG AAGATCCTCC AGCCTCAGGT GTTTAGCATG
    AGCTTTAACA GACATAATCT GAAATACTAC GTGTTACCAA AAAAACCCAA GAAGGTGGCA
    TTTGATTGCT TAGAATGGAT CAGAAAGTAT CACCCACATG ACTCCGGGAT AATCTATTGC
    CTCTCCAGAC GAGAATGTGA TACAATGGCT GACACTTTAC AGAAAGATGG TCTTGCTGCT
    CTTGCTTATC ATGCTGGCCT CAGTGATTCT GCAAGGGATG AAGTACAGCA CAAGTGGATT
    AATCAGGATG GCTGCCAGGT CATCTGTGCA ACAATAGCGT TCGGAATGGG GATTGACAAA
    CCTGATGTGC GATTCGTGAT TCATGCATCT CTCCCTAAGT CTGTGGAGGG CTACTACCAA
    GAATCTGGCA GAGCTGGAAG AGATGGGGAA ATTTCTCACT GCCTGCTGTT CTATACCTAT
    CATGATGTGA CACGACTCAA GAGACTCATC CTGATGGAAA AAGATGGAAA CCGTCATACA
    AGAGAGACTC ACTTCAATAA TTTGTATAGT ATGGTGCATT ACTGTGAAAA TATAACAGAA
    TGCAGGAGAA TACAGCTTTT GGCTTACTTT GGCGAAAGTG GATTTAATCC TGATTTTTGT
    AAGAAATACC CTGATGTTTC TTGTGATAAT TGCTGTAAAA CAAAGGATTA TAAGACAAGA
    GATGTGACTG ATGATGTAAA AAATATTGTA AGATTTGTTC AAGAACATAG TTCATCACAA
    GGAACAAGAA TTAAAAACCA ATTAGGTCCT TCTGGGAGAT TTACTATGAA TATGCTAGTT
    GACATTTTCT TGGGGAGTAA GAGTGCAAAA ATTCAGTCGG GGATATTTGG ACAGGGATCT
    ACTTATTCAC GACACAATGC CGAAAGACTT TTTAAGAAAT TGATACTTGA CAAGATTTTG
    GATGAAGACT TATATATCAA TGCCAATGAC CAACCAATTG CGTATGTGAT GCCAGGAAAT
    AAAGCCCAAA CTGTACTAAA TGGGCATTTA AAGGTAGACT TTATAGAAAC GGAAAATTCT
    ACCAGTATAA AAAAACAGAA AGCTGCTTTA GTGGCAAAGA TGTCTCAGAG AGAAGAAGTG
    GTAAAGAAGT GTCTTGGAGA ACTGACAGAA GTGTGTAAAT CTCTGGGAAA AGTTTTTGGT
    GTCCACTACT TTAATATTTT TAATACTGTG ACTCTCAAGA AGCTTGCAGA ATCTCTATCT
    TCTGATCCCG AGGTTTTGCT TCAAATTGAT GGTGTTACTG AAGACAAACT GGAAAAATAT
    GGTGCAGAAG TGATTCCAAT ATTACAGAAA TACTCTGAGT GGACATTGCC AGCTGACGAT
    GGTTCCCCAC GAATCAGCCC GAGCAGCAGC AGAAGCACCA GGAGAAGTGC CCCTGAGGAG
    TTCGACGAGG AAACATCCGT ATCTTCTCAC TACTTTGCAA ATAAAACCAA AAATGAAAGA
    AAGAGGAAAA GGATGTCAGC CACCCAGAGG TCTAAGAGGA GGAGAACTGG CTTTGGTGGC
    TCCAAGACAA AAGGGGGGTC CACCGCATGC AGAAAGACAT CTTCTAAAAG TAAATCCTCC
    AACATATTTG GACCTCATTC ATCTTTGCAT GGTTCTCAAG CAGCATCAGG AGCCGCTAGA
    AAATTGGGGA TTATGGCTCC CCCAAAGCCT ATAAATAGAC CGTTTCTTAA GCCTTCATAT
    GCATTTTCAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272657.1
    CDS104..4375
    Translation

    Target ORF information:

    RefSeq Version XM_022416949.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Bloom syndrome RecQ like helicase (BLM), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416949.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGCCG TTCCCCAGAA TAATCTACAG GAGCAACTAG AACGCCATTC AGCCAGAAAA 
    CTTAGTAACA AATTAAGTCC TTCCAAACCG AAATCTTCAG TTTTCACTTT TAAGAAGAAA
    ACATCAGCCA ACAATGTGTC TGTCACTAGT GTGTCAGTAG CAAAAACACC TGTGTTAAAT
    GACAAAGATG TTAATGTCAC TGAGGCCTTT CCCTTCAGTG AACCTCTGCC CCACGCCACA
    AGTCAGCAGA CAAGGCCCAG TGACCTCAAA AATACTCCAG CAGGACAGCA AACCAGGAGA
    ACTGTTTCAA AACCATTATT GCCAGATTTG TCACTGGTTT CACGAGTTTT ATGTAATACC
    CAGAACACAC CGATTGTAAA GAAATCCAGT AATGCTACTT TCAAGAAATT GGAATTTAGC
    TCTTCATCAG ATTCTTTTGT TACCATCAAT GATTGGGATG ATATGGATGA CTTTGATACT
    TCTGGAAATT CAAAGCTTGT TACACCATGC AAAAATCACT TTGTGAGAGT TAGCACTGCT
    CAGAAGTCAA AAAAACCTAA GAGAAACTTT TTGAAAGCAC AGCTTCCTCA AGCAAATACA
    GTAAAGGCTG ATTTGACTCC ATCCTCCTTT GAAAGTAAGC AAGCATGTTT GACTAAAAAA
    CAGAACGACG ACTCGGAATG GTTTAGTAAT GATGTGATTT GCATTGATGA TGACCCCAAT
    CCTGAAGAGC TTATACATGG AGATCCTCAG GAGAGTCATT CTTTAAAAAC TCATTTGGAA
    GAGGAAAGAG ATAGCGAAAA GAAGAACTGG GAAGAAACAG AGTTATGTTC ATTGGAGAAG
    TCGCCATGTG TAGAGCTTGA CGAAGATGAT TACGATACAG ATTTTGTTCC ACCTTCTCCA
    GAAGTAATTT CTGCCTCTTC TTCCTCTTTA AAGTGTTTTA GTATGTTAAA GGACCTTGAT
    GCCTCTGGCA CAGAGGATGG TCCTAGCACA TCCGAGGACT TGCCAACACC CAAGGGGATG
    ACAACACAGC AGCCCAATCA AGAGACCAGC ACAAACTGTG ACGGTACAAA AACTAGACAG
    CCAAGTTTAC AGCAGCAGCT TATTCACGTG ATGGACCAGA TCTGTAAATT AGTAGATACT
    ATTCCTGATG ATGAACTGAA AGCTCTGACT TGTGGGAATG CACTGCATCA GCAGCGGGAC
    ATAAGAAGGA AGCTCCTAGC TGAAGCCGAC TTTAATACAA GTGATGCCAG TGTTCTTGCT
    TCGGTGTGGA CACGCGGGCC TGATTCACTG GGAAGCCCTG TGGAGGATAT GGCATCTCTT
    TCTGCTCCTG ATGTGTTTCC AAAGGGCAAT TCCCATCCTA CCGGGAATTC TGTTAAGGAG
    TTAAATTTTC CACACCTTCC CTCACATTCC AGTTCCACTG GGGAGTGTTT ACTGGCTACT
    ACCCCAGGAA AGACAGGATT CTCCGGCACC ATGAAGAATC CCTCTGAAAG ACTGTTATTC
    AGTCCCCATT TACAGAAGTC CTTTGTAAGT AGCAACTGGG CTGAAACACC ACGGATAGAA
    AAAAGGAACG AGAGCTCTTA CTTCCTGGGA AATGTTCTCA CAAGCACGGC TGTGAGAGAT
    CAGAATAAAC GTGCTGCTCC AATAAATGAC TTAGAAATTC AGGCTGCCTG TGATATTGAT
    AATTTTGACA TAGATGGCTT TGATGATGAT GATGACTGGG AAGATATAAT GCATGATTTA
    GCGGCCAGCA GATCTTCCAC AGCTGCCTGT CTACCTATTA AGGAAGGTCG GCCAGTTAAG
    CCAGTATCAG AAAGAATTTC TTCAGCCAAG ACAAACTGTC TTCCAGTGGC ATCTACTGCT
    CAAAATAAAA ACTCAGAGTC AATTCAGAAT TACACTGACA AGTCGGCGCA AAATTTAGCA
    TCCAGAAATC TGAAACATGA ACGTTTCCAA AGTCTTAGTT TTCCTCATAC AAAAGAAATG
    ATGAAGATTT TTCATAAAAA ATTTGGCCTG CATAATTTTA GAACTAATCA GCTAGAGGCG
    ATCAATGCTG CACTGCTGGG TGAAGACTGT TTTATCTTAA TGCCCACTGG AGGTGGTAAG
    AGTCTGTGTT ACCAGCTCCC TGCCTGTGTC TCTCCTGGTG TCACTATTGT CATTTCTCCT
    TTGAGATCAC TTATAGTAGA TCAAGTCCAA AAGCTGACTT CCTTGGATAT TCCAGCTACA
    TATCTGACAG GTGATAAGAC TGACTCGGAA GCAACAAGTA TTTACCTGCA ATTATCCAAG
    AAAGACCCAA TTATAAAACT TCTATATGTT ACTCCAGAGA AGGTCTGTGC AAGTAATAGA
    CTGCTTTCCA CTCTGGAGAA TCTGTATGAG AGGAAGCTCC TGGCACGTTT TGTCATTGAT
    GAAGCGCATT GTGTCAGTCA GTGGGGACAT GATTTTCGCC AAGATTATAA AAGAATGAAT
    ATGCTTCGCC AGAAGTTTCC CTCTGTTCCA GTGATGGCCC TTACTGCCAC AGCTAATCCC
    AGGGTACAGA AGGACATCCT CACTCAGCTG AAGATCCTCC AGCCTCAGGT GTTTAGCATG
    AGCTTTAACA GACATAATCT GAAATACTAC GTGTTACCAA AAAAACCCAA GAAGGTGGCA
    TTTGATTGCT TAGAATGGAT CAGAAAGTAT CACCCACATG ACTCCGGGAT AATCTATTGC
    CTCTCCAGAC GAGAATGTGA TACAATGGCT GACACTTTAC AGAAAGATGG TCTTGCTGCT
    CTTGCTTATC ATGCTGGCCT CAGTGATTCT GCAAGGGATG AAGTACAGCA CAAGTGGATT
    AATCAGGATG GCTGCCAGGT CATCTGTGCA ACAATAGCGT TCGGAATGGG GATTGACAAA
    CCTGATGTGC GATTCGTGAT TCATGCATCT CTCCCTAAGT CTGTGGAGGG CTACTACCAA
    GAATCTGGCA GAGCTGGAAG AGATGGGGAA ATTTCTCACT GCCTGCTGTT CTATACCTAT
    CATGATGTGA CACGACTCAA GAGACTCATC CTGATGGAAA AAGATGGAAA CCGTCATACA
    AGAGAGACTC ACTTCAATAA TTTGTATAGT ATGGTGCATT ACTGTGAAAA TATAACAGAA
    TGCAGGAGAA TACAGCTTTT GGCTTACTTT GGCGAAAGTG GATTTAATCC TGATTTTTGT
    AAGAAATACC CTGATGTTTC TTGTGATAAT TGCTGTAAAA CAAAGGATTA TAAGACAAGA
    GATGTGACTG ATGATGTAAA AAATATTGTA AGATTTGTTC AAGAACATAG TTCATCACAA
    GGAACAAGAA TTAAAAACCA ATTAGGTCCT TCTGGGAGAT TTACTATGAA TATGCTAGTT
    GACATTTTCT TGGGGAGTAA GAGTGCAAAA ATTCAGTCGG GGATATTTGG ACAGGGATCT
    ACTTATTCAC GACACAATGC CGAAAGACTT TTTAAGAAAT TGATACTTGA CAAGATTTTG
    GATGAAGACT TATATATCAA TGCCAATGAC CAACCAATTG CGTATGTGAT GCCAGGAAAT
    AAAGCCCAAA CTGTACTAAA TGGGCATTTA AAGGTAGACT TTATAGAAAC GGAAAATTCT
    ACCAGTATAA AAAAACAGAA AGCTGCTTTA GTGGCAAAGA TGTCTCAGAG AGAAGAAGTG
    GTAAAGAAGT GTCTTGGAGA ACTGACAGAA GTGTGTAAAT CTCTGGGAAA AGTTTTTGGT
    GTCCACTACT TTAATATTTT TAATACTGTG ACTCTCAAGA AGCTTGCAGA ATCTCTATCT
    TCTGATCCCG AGGTTTTGCT TCAAATTGAT GGTGTTACTG AAGACAAACT GGAAAAATAT
    GGTGCAGAAG TGATTCCAAT ATTACAGAAA TACTCTGAGT GGACATTGCC AGCTGACGAT
    GGTTCCCCAC GAATCAGCCC GAGCAGCAGC AGAAGCACCA GGAGAAGTGC CCCTGAGGAG
    TTCGACGAGG AAACATCCGT ATCTTCTCAC TACTTTGCAA ATAAAACCAA AAATGAAAGA
    AAGAGGAAAA GGATGTCAGC CACCCAGAGG TCTAAGAGGA GGAGAACTGG CTTTGGTGGC
    TCCAAGACAA AAGGGGGGTC CACCGCATGC AGAAAGACAT CTTCTAAAAG TAAATCCTCC
    AACATATTTG GACCTCATTC ATCTTTGCAT GGTTCTCAAG CAGCATCAGG AGCCGCTAGA
    AAATTGGGGA TTATGGCTCC CCCAAAGCCT ATAAATAGAC CGTTTCTTAA GCCTTCATAT
    GCATTTTCAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287232
    Clone ID Related Accession (Same CDS sequence) XM_022416951.1
    Accession Version XM_022416951.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product Bloom syndrome protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCTGCCG TTCCCCAGAA TAATCTACAG GAGCAACTAG AACGCCATTC AGCCAGAAAA 
    CTTAGTAACA AATTAAGTCC TTCCAAACCG AAATCTTCAG TTTTCACTTT TAAGAAGAAA
    ACATCAGCCA ACAATGTGTC TGTCACTAGT GTGTCAGTAG CAAAAACACC TGTGTTAAAT
    GACAAAGATG TTAATGTCAC TGAGGCCTTT CCCTTCAGTG AACCTCTGCC CCACGCCACA
    AGTCAGCAGA CAAGGCCCAG TGACCTCAAA AATACTCCAG CAGGACAGCA AACCAGGAGA
    ACTGTTTCAA AACCATTATT GCCAGATTTG TCACTGGTTT CACGAGTTTT ATGTAATACC
    CAGAACACAC CGATTGTAAA GAAATCCAGT AATGCTACTT TCAAGAAATT GGAATTTAGC
    TCTTCATCAG ATTCTTTTGT TACCATCAAT GATTGGGATG ATATGGATGA CTTTGATACT
    TCTGGAAATT CAAAGCTTGT TACACCATGC AAAAATCACT TTGTGAGAGT TAGCACTGCT
    CAGAAGTCAA AAAAACCTAA GAGAAACTTT TTGAAAGCAC AGCTTCCTCA AGCAAATACA
    GTAAAGGCTG ATTTGACTCC ATCCTCCTTT GAAAGTAAGC AAGCATGTTT GACTAAAAAA
    CAGAACGACG ACTCGGAATG GTTTAGTAAT GATGTGATTT GCATTGATGA TGACCCCAAT
    CCTGAAGAGC TTATACATGG AGATCCTCAG GAGAGTCATT CTTTAAAAAC TCATTTGGAA
    GAGGAAAGAG ATAGCGAAAA GAAGAACTGG GAAGAAACAG AGTTATGTTC ATTGGAGAAG
    TCGCCATGTG TAGAGCTTGA CGAAGATGAT TACGATACAG ATTTTGTTCC ACCTTCTCCA
    GAAGTAATTT CTGCCTCTTC TTCCTCTTTA AAGTGTTTTA GTATGTTAAA GGACCTTGAT
    GCCTCTGGCA CAGAGGATGG TCCTAGCACA TCCGAGGACT TGCCAACACC CAAGGGGATG
    ACAACACAGC AGCCCAATCA AGAGACCAGC ACAAACTGTG ACGGTACAAA AACTAGACAG
    CCAAGTTTAC AGCAGCAGCT TATTCACGTG ATGGACCAGA TCTGTAAATT AGTAGATACT
    ATTCCTGATG ATGAACTGAA AGCTCTGACT TGTGGGAATG CACTGCATCA GCAGCGGGAC
    ATAAGAAGGA AGCTCCTAGC TGAAGCCGAC TTTAATACAA GTGATGCCAG TGTTCTTGCT
    TCGGTGTGGA CACGCGGGCC TGATTCACTG GGAAGCCCTG TGGAGGATAT GGCATCTCTT
    TCTGCTCCTG ATGTGTTTCC AAAGGGCAAT TCCCATCCTA CCGGGAATTC TGTTAAGGAG
    TTAAATTTTC CACACCTTCC CTCACATTCC AGTTCCACTG GGGAGTGTTT ACTGGCTACT
    ACCCCAGGAA AGACAGGATT CTCCGGCACC ATGAAGAATC CCTCTGAAAG ACTGTTATTC
    AGTCCCCATT TACAGAAGTC CTTTGTAAGT AGCAACTGGG CTGAAACACC ACGGATAGAA
    AAAAGGAACG AGAGCTCTTA CTTCCTGGGA AATGTTCTCA CAAGCACGGC TGTGAGAGAT
    CAGAATAAAC GTGCTGCTCC AATAAATGAC TTAGAAATTC AGGCTGCCTG TGATATTGAT
    AATTTTGACA TAGATGGCTT TGATGATGAT GATGACTGGG AAGATATAAT GCATGATTTA
    GCGGCCAGCA GATCTTCCAC AGCTGCCTGT CTACCTATTA AGGAAGGTCG GCCAGTTAAG
    CCAGTATCAG AAAGAATTTC TTCAGCCAAG ACAAACTGTC TTCCAGTGGC ATCTACTGCT
    CAAAATAAAA ACTCAGAGTC AATTCAGAAT TACACTGGAG GTGGTAAGAG TCTGTGTTAC
    CAGCTCCCTG CCTGTGTCTC TCCTGGTGTC ACTATTGTCA TTTCTCCTTT GAGATCACTT
    ATAGTAGATC AAGTCCAAAA GCTGACTTCC TTGGATATTC CAGCTACATA TCTGACAGGT
    GATAAGACTG ACTCGGAAGC AACAAGTATT TACCTGCAAT TATCCAAGAA AGACCCAATT
    ATAAAACTTC TATATGTTAC TCCAGAGAAG GTCTGTGCAA GTAATAGACT GCTTTCCACT
    CTGGAGAATC TGTATGAGAG GAAGCTCCTG GCACGTTTTG TCATTGATGA AGCGCATTGT
    GTCAGTCAGT GGGGACATGA TTTTCGCCAA GATTATAAAA GAATGAATAT GCTTCGCCAG
    AAGTTTCCCT CTGTTCCAGT GATGGCCCTT ACTGCCACAG CTAATCCCAG GGTACAGAAG
    GACATCCTCA CTCAGCTGAA GATCCTCCAG CCTCAGGTGT TTAGCATGAG CTTTAACAGA
    CATAATCTGA AATACTACGT GTTACCAAAA AAACCCAAGA AGGTGGCATT TGATTGCTTA
    GAATGGATCA GAAAGTATCA CCCACATGAC TCCGGGATAA TCTATTGCCT CTCCAGACGA
    GAATGTGATA CAATGGCTGA CACTTTACAG AAAGATGGTC TTGCTGCTCT TGCTTATCAT
    GCTGGCCTCA GTGATTCTGC AAGGGATGAA GTACAGCACA AGTGGATTAA TCAGGATGGC
    TGCCAGGTCA TCTGTGCAAC AATAGCGTTC GGAATGGGGA TTGACAAACC TGATGTGCGA
    TTCGTGATTC ATGCATCTCT CCCTAAGTCT GTGGAGGGCT ACTACCAAGA ATCTGGCAGA
    GCTGGAAGAG ATGGGGAAAT TTCTCACTGC CTGCTGTTCT ATACCTATCA TGATGTGACA
    CGACTCAAGA GACTCATCCT GATGGAAAAA GATGGAAACC GTCATACAAG AGAGACTCAC
    TTCAATAATT TGTATAGTAT GGTGCATTAC TGTGAAAATA TAACAGAATG CAGGAGAATA
    CAGCTTTTGG CTTACTTTGG CGAAAGTGGA TTTAATCCTG ATTTTTGTAA GAAATACCCT
    GATGTTTCTT GTGATAATTG CTGTAAAACA AAGGATTATA AGACAAGAGA TGTGACTGAT
    GATGTAAAAA ATATTGTAAG ATTTGTTCAA GAACATAGTT CATCACAAGG AACAAGAATT
    AAAAACCAAT TAGGTCCTTC TGGGAGATTT ACTATGAATA TGCTAGTTGA CATTTTCTTG
    GGGAGTAAGA GTGCAAAAAT TCAGTCGGGG ATATTTGGAC AGGGATCTAC TTATTCACGA
    CACAATGCCG AAAGACTTTT TAAGAAATTG ATACTTGACA AGATTTTGGA TGAAGACTTA
    TATATCAATG CCAATGACCA ACCAATTGCG TATGTGATGC CAGGAAATAA AGCCCAAACT
    GTACTAAATG GGCATTTAAA GGTAGACTTT ATAGAAACGG AAAATTCTAC CAGTATAAAA
    AAACAGAAAG CTGCTTTAGT GGCAAAGATG TCTCAGAGAG AAGAAGTGGT AAAGAAGTGT
    CTTGGAGAAC TGACAGAAGT GTGTAAATCT CTGGGAAAAG TTTTTGGTGT CCACTACTTT
    AATATTTTTA ATACTGTGAC TCTCAAGAAG CTTGCAGAAT CTCTATCTTC TGATCCCGAG
    GTTTTGCTTC AAATTGATGG TGTTACTGAA GACAAACTGG AAAAATATGG TGCAGAAGTG
    ATTCCAATAT TACAGAAATA CTCTGAGTGG ACATTGCCAG CTGACGATGG TTCCCCACGA
    ATCAGCCCGA GCAGCAGCAG AAGCACCAGG AGAAGTGCCC CTGAGGAGTT CGACGAGGAA
    ACATCCGTAT CTTCTCACTA CTTTGCAAAT AAAACCAAAA ATGAAAGAAA GAGGAAAAGG
    ATGTCAGCCA CCCAGAGGTC TAAGAGGAGG AGAACTGGCT TTGGTGGCTC CAAGACAAAA
    GGGGGGTCCA CCGCATGCAG AAAGACATCT TCTAAAAGTA AATCCTCCAA CATATTTGGA
    CCTCATTCAT CTTTGCATGG TTCTCAAGCA GCATCAGGAG CCGCTAGAAA ATTGGGGATT
    ATGGCTCCCC CAAAGCCTAT AAATAGACCG TTTCTTAAGC CTTCATATGC ATTTTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272659.1
    CDS104..4183
    Translation

    Target ORF information:

    RefSeq Version XM_022416951.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Bloom syndrome RecQ like helicase (BLM), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416951.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCTGCCG TTCCCCAGAA TAATCTACAG GAGCAACTAG AACGCCATTC AGCCAGAAAA 
    CTTAGTAACA AATTAAGTCC TTCCAAACCG AAATCTTCAG TTTTCACTTT TAAGAAGAAA
    ACATCAGCCA ACAATGTGTC TGTCACTAGT GTGTCAGTAG CAAAAACACC TGTGTTAAAT
    GACAAAGATG TTAATGTCAC TGAGGCCTTT CCCTTCAGTG AACCTCTGCC CCACGCCACA
    AGTCAGCAGA CAAGGCCCAG TGACCTCAAA AATACTCCAG CAGGACAGCA AACCAGGAGA
    ACTGTTTCAA AACCATTATT GCCAGATTTG TCACTGGTTT CACGAGTTTT ATGTAATACC
    CAGAACACAC CGATTGTAAA GAAATCCAGT AATGCTACTT TCAAGAAATT GGAATTTAGC
    TCTTCATCAG ATTCTTTTGT TACCATCAAT GATTGGGATG ATATGGATGA CTTTGATACT
    TCTGGAAATT CAAAGCTTGT TACACCATGC AAAAATCACT TTGTGAGAGT TAGCACTGCT
    CAGAAGTCAA AAAAACCTAA GAGAAACTTT TTGAAAGCAC AGCTTCCTCA AGCAAATACA
    GTAAAGGCTG ATTTGACTCC ATCCTCCTTT GAAAGTAAGC AAGCATGTTT GACTAAAAAA
    CAGAACGACG ACTCGGAATG GTTTAGTAAT GATGTGATTT GCATTGATGA TGACCCCAAT
    CCTGAAGAGC TTATACATGG AGATCCTCAG GAGAGTCATT CTTTAAAAAC TCATTTGGAA
    GAGGAAAGAG ATAGCGAAAA GAAGAACTGG GAAGAAACAG AGTTATGTTC ATTGGAGAAG
    TCGCCATGTG TAGAGCTTGA CGAAGATGAT TACGATACAG ATTTTGTTCC ACCTTCTCCA
    GAAGTAATTT CTGCCTCTTC TTCCTCTTTA AAGTGTTTTA GTATGTTAAA GGACCTTGAT
    GCCTCTGGCA CAGAGGATGG TCCTAGCACA TCCGAGGACT TGCCAACACC CAAGGGGATG
    ACAACACAGC AGCCCAATCA AGAGACCAGC ACAAACTGTG ACGGTACAAA AACTAGACAG
    CCAAGTTTAC AGCAGCAGCT TATTCACGTG ATGGACCAGA TCTGTAAATT AGTAGATACT
    ATTCCTGATG ATGAACTGAA AGCTCTGACT TGTGGGAATG CACTGCATCA GCAGCGGGAC
    ATAAGAAGGA AGCTCCTAGC TGAAGCCGAC TTTAATACAA GTGATGCCAG TGTTCTTGCT
    TCGGTGTGGA CACGCGGGCC TGATTCACTG GGAAGCCCTG TGGAGGATAT GGCATCTCTT
    TCTGCTCCTG ATGTGTTTCC AAAGGGCAAT TCCCATCCTA CCGGGAATTC TGTTAAGGAG
    TTAAATTTTC CACACCTTCC CTCACATTCC AGTTCCACTG GGGAGTGTTT ACTGGCTACT
    ACCCCAGGAA AGACAGGATT CTCCGGCACC ATGAAGAATC CCTCTGAAAG ACTGTTATTC
    AGTCCCCATT TACAGAAGTC CTTTGTAAGT AGCAACTGGG CTGAAACACC ACGGATAGAA
    AAAAGGAACG AGAGCTCTTA CTTCCTGGGA AATGTTCTCA CAAGCACGGC TGTGAGAGAT
    CAGAATAAAC GTGCTGCTCC AATAAATGAC TTAGAAATTC AGGCTGCCTG TGATATTGAT
    AATTTTGACA TAGATGGCTT TGATGATGAT GATGACTGGG AAGATATAAT GCATGATTTA
    GCGGCCAGCA GATCTTCCAC AGCTGCCTGT CTACCTATTA AGGAAGGTCG GCCAGTTAAG
    CCAGTATCAG AAAGAATTTC TTCAGCCAAG ACAAACTGTC TTCCAGTGGC ATCTACTGCT
    CAAAATAAAA ACTCAGAGTC AATTCAGAAT TACACTGGAG GTGGTAAGAG TCTGTGTTAC
    CAGCTCCCTG CCTGTGTCTC TCCTGGTGTC ACTATTGTCA TTTCTCCTTT GAGATCACTT
    ATAGTAGATC AAGTCCAAAA GCTGACTTCC TTGGATATTC CAGCTACATA TCTGACAGGT
    GATAAGACTG ACTCGGAAGC AACAAGTATT TACCTGCAAT TATCCAAGAA AGACCCAATT
    ATAAAACTTC TATATGTTAC TCCAGAGAAG GTCTGTGCAA GTAATAGACT GCTTTCCACT
    CTGGAGAATC TGTATGAGAG GAAGCTCCTG GCACGTTTTG TCATTGATGA AGCGCATTGT
    GTCAGTCAGT GGGGACATGA TTTTCGCCAA GATTATAAAA GAATGAATAT GCTTCGCCAG
    AAGTTTCCCT CTGTTCCAGT GATGGCCCTT ACTGCCACAG CTAATCCCAG GGTACAGAAG
    GACATCCTCA CTCAGCTGAA GATCCTCCAG CCTCAGGTGT TTAGCATGAG CTTTAACAGA
    CATAATCTGA AATACTACGT GTTACCAAAA AAACCCAAGA AGGTGGCATT TGATTGCTTA
    GAATGGATCA GAAAGTATCA CCCACATGAC TCCGGGATAA TCTATTGCCT CTCCAGACGA
    GAATGTGATA CAATGGCTGA CACTTTACAG AAAGATGGTC TTGCTGCTCT TGCTTATCAT
    GCTGGCCTCA GTGATTCTGC AAGGGATGAA GTACAGCACA AGTGGATTAA TCAGGATGGC
    TGCCAGGTCA TCTGTGCAAC AATAGCGTTC GGAATGGGGA TTGACAAACC TGATGTGCGA
    TTCGTGATTC ATGCATCTCT CCCTAAGTCT GTGGAGGGCT ACTACCAAGA ATCTGGCAGA
    GCTGGAAGAG ATGGGGAAAT TTCTCACTGC CTGCTGTTCT ATACCTATCA TGATGTGACA
    CGACTCAAGA GACTCATCCT GATGGAAAAA GATGGAAACC GTCATACAAG AGAGACTCAC
    TTCAATAATT TGTATAGTAT GGTGCATTAC TGTGAAAATA TAACAGAATG CAGGAGAATA
    CAGCTTTTGG CTTACTTTGG CGAAAGTGGA TTTAATCCTG ATTTTTGTAA GAAATACCCT
    GATGTTTCTT GTGATAATTG CTGTAAAACA AAGGATTATA AGACAAGAGA TGTGACTGAT
    GATGTAAAAA ATATTGTAAG ATTTGTTCAA GAACATAGTT CATCACAAGG AACAAGAATT
    AAAAACCAAT TAGGTCCTTC TGGGAGATTT ACTATGAATA TGCTAGTTGA CATTTTCTTG
    GGGAGTAAGA GTGCAAAAAT TCAGTCGGGG ATATTTGGAC AGGGATCTAC TTATTCACGA
    CACAATGCCG AAAGACTTTT TAAGAAATTG ATACTTGACA AGATTTTGGA TGAAGACTTA
    TATATCAATG CCAATGACCA ACCAATTGCG TATGTGATGC CAGGAAATAA AGCCCAAACT
    GTACTAAATG GGCATTTAAA GGTAGACTTT ATAGAAACGG AAAATTCTAC CAGTATAAAA
    AAACAGAAAG CTGCTTTAGT GGCAAAGATG TCTCAGAGAG AAGAAGTGGT AAAGAAGTGT
    CTTGGAGAAC TGACAGAAGT GTGTAAATCT CTGGGAAAAG TTTTTGGTGT CCACTACTTT
    AATATTTTTA ATACTGTGAC TCTCAAGAAG CTTGCAGAAT CTCTATCTTC TGATCCCGAG
    GTTTTGCTTC AAATTGATGG TGTTACTGAA GACAAACTGG AAAAATATGG TGCAGAAGTG
    ATTCCAATAT TACAGAAATA CTCTGAGTGG ACATTGCCAG CTGACGATGG TTCCCCACGA
    ATCAGCCCGA GCAGCAGCAG AAGCACCAGG AGAAGTGCCC CTGAGGAGTT CGACGAGGAA
    ACATCCGTAT CTTCTCACTA CTTTGCAAAT AAAACCAAAA ATGAAAGAAA GAGGAAAAGG
    ATGTCAGCCA CCCAGAGGTC TAAGAGGAGG AGAACTGGCT TTGGTGGCTC CAAGACAAAA
    GGGGGGTCCA CCGCATGCAG AAAGACATCT TCTAAAAGTA AATCCTCCAA CATATTTGGA
    CCTCATTCAT CTTTGCATGG TTCTCAAGCA GCATCAGGAG CCGCTAGAAA ATTGGGGATT
    ATGGCTCCCC CAAAGCCTAT AAATAGACCG TTTCTTAAGC CTTCATATGC ATTTTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287233
    Clone ID Related Accession (Same CDS sequence) XM_022416952.1
    Accession Version XM_022416952.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product Bloom syndrome protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCTGCCG TTCCCCAGAA TAATCTACAG GAGCAACTAG AACGCCATTC AGCCAGAAAA 
    CTTAGTAACA AATTAAGTCC TTCCAAACCG AAATCTTCAG TTTTCACTTT TAAGAAGAAA
    ACATCAGCCA ACAATGTGTC TGTCACTAGT GTGTCAGTAG CAAAAACACC TGTGTTAAAT
    GACAAAGATG TTAATGTCAC TGAGGCCTTT CCCTTCAGTG AACCTCTGCC CCACGCCACA
    AGTCAGCAGA CAAGGCCCAG TGACCTCAAA AATACTCCAG CAGGACAGCA AACCAGGAGA
    ACTGTTTCAA AACCATTATT GCCAGATTTG TCACTGGTTT CACGAGTTTT ATGTAATACC
    CAGAACACAC CGATTGTAAA GAAATCCAGT AATGCTACTT TCAAGAAATT GGAATTTAGC
    TCTTCATCAG ATTCTTTTGT TACCATCAAT GATTGGGATG ATATGGATGA CTTTGATACT
    TCTGGAAATT CAAAGCTTGT TACACCATGC AAAAATCACT TTGTGAGAGT TAGCACTGCT
    CAGAAGTCAA AAAAACCTAA GAGAAACTTT TTGAAAGCAC AGCTTCCTCA AGCAAATACA
    GTAAAGGCTG ATTTGACTCC ATCCTCCTTT GAAAGTAAGC AAGCATGTTT GACTAAAAAA
    CAGAACGACG ACTCGGAATG GTTTAGTAAT GATGTGATTT GCATTGATGA TGACCCCAAT
    CCTGAAGAGC TTATACATGG AGATCCTCAG GAGAGTCATT CTTTAAAAAC TCATTTGGAA
    GAGGAAAGAG ATAGCGAAAA GAAGAACTGG GAAGAAACAG AGTTATGTTC ATTGGAGAAG
    TCGCCATGTG TAGAGCTTGA CGAAGATGAT TACGATACAG ATTTTGTTCC ACCTTCTCCA
    GAAGTAATTT CTGCCTCTTC TTCCTCTTTA AAGTGTTTTA GTATGTTAAA GGACCTTGAT
    GCCTCTGGCA CAGAGGATGG TCCTAGCACA TCCGAGGACT TGCCAACACC CAAGGGGATG
    ACAACACAGC AGCCCAATCA AGAGACCAGC ACAAACTGTG ACGGTACAAA AACTAGACAG
    CCAAGTTTAC AGCAGCAGCT TATTCACGTG ATGGACCAGA TCTGTAAATT AGTAGATACT
    ATTCCTGATG ATGAACTGAA AGCTCTGACT TGTGGGAATG CACTGCATCA GCAGCGGGAC
    ATAAGAAGGA AGCTCCTAGC TGAAGCCGAC TTTAATACAA GTGATGCCAG TGTTCTTGCT
    TCGGTGTGGA CACGCGGGCC TGATTCACTG GGAAGCCCTG TGGAGGATAT GGCATCTCTT
    TCTGCTCCTG ATGTGTTTCC AAAGGGCAAT TCCCATCCTA CCGGGAATTC TGTTAAGGAG
    TTAAATTTTC CACACCTTCC CTCACATTCC AGTTCCACTG GGGAGTGTTT ACTGGCTACT
    ACCCCAGGAA AGACAGGATT CTCCGGCACC ATGAAGAATC CCTCTGAAAG ACTGTTATTC
    AGTCCCCATT TACAGAAGTC CTTTGTAAGT AGCAACTGGG CTGAAACACC ACGGATAGAA
    AAAAGGAACG AGAGCTCTTA CTTCCTGGGA AATGTTCTCA CAAGCACGGC TGTGAGAGAT
    CAGAATAAAC GTGCTGCTCC AATAAATGAC TTAGAAATTC AGGCTGCCTG TGATATTGAT
    AATTTTGACA TAGATGGCTT TGATGATGAT GATGACTGGG AAGATATAAT GCATGATTTA
    GCGGCCAGCA GATCTTCCAC AGCTGCCTGT CTACCTATTA AGGAAGGTCG GCCAGTTAAG
    CCAGTATCAG AAAGAATTTC TTCAGCCAAG ACAAACTGTC TTCCAGTGGC ATCTACTGCT
    CAAAATAAAA ACTCAGAGTC AATTCAGAAT TACACTGACA AGTCGGCGCA AAATTTAGCA
    TCCAGAAATC TGAAACATGA ACGTTTCCAA AGTCTTAGTT TTCCTCATAC AAAAGAAATG
    ATGAAGATTT TTCATAAAAA ATTTGGCCTG CATAATTTTA GAACTAATCA GCTAGAGGCG
    ATCAATGCTG CACTGCTGGG TGAAGACTGT TTTATCTTAA TGCCCACTGG AGGTGGTAAG
    AGTCTGTGTT ACCAGCTCCC TGCCTGTGTC TCTCCTGGTG TCACTATTGT CATTTCTCCT
    TTGAGATCAC TTATAGTAGA TCAAGTCCAA AAGCTGACTT CCTTGGATAT TCCAGCTACA
    TATCTGACAG GTGATAAGAC TGACTCGGAA GCAACAAGTA TTTACCTGCA ATTATCCAAG
    AAAGACCCAA TTATAAAACT TCTATATGTT ACTCCAGAGA AGGTCTGTGC AAGTAATAGA
    CTGCTTTCCA CTCTGGAGAA TCTGTATGAG AGGAAGCTCC TGGCACGTTT TGTCATTGAT
    GAAGCGCATT GTGTCAGTCA GTGGGGACAT GATTTTCGCC AAGATTATAA AAGAATGAAT
    ATGCTTCGCC AGAAGTTTCC CTCTGTTCCA GTGATGGCCC TTACTGCCAC AGCTAATCCC
    AGGGTACAGA AGGACATCCT CACTCAGCTG AAGATCCTCC AGCCTCAGGT GTTTAGCATG
    AGCTTTAACA GACATAATCT GAAATACTAC GTGTTACCAA AAAAACCCAA GAAGGTGGCA
    TTTGATTGCT TAGAATGGAT CAGAAAGTAT CACCCACATG ACTCCGGGAT AATCTATTGC
    CTCTCCAGAC GAGAATGTGA TACAATGGCT GACACTTTAC AGAAAGATGG TCTTGCTGCT
    CTTGCTTATC ATGCTGGCCT CAGTGATTCT GCAAGGGATG AAGTACAGCA CAAGTGGATT
    AATCAGGATG GCTGCCAGGT CATCTGTGCA ACAATAGCGT TCGGAATGGG GATTGACAAA
    CCTGATGTGC GATTCGTGAT TCATGCATCT CTCCCTAAGT CTGTGGAGGG CTACTACCAA
    GAATCTGGCA GAGCTGGAAG AGATGGGGAA ATTTCTCACT GCCTGCTGTT CTATACCTAT
    CATGATGTGA CACGACTCAA GAGACTCATC CTGATGGAAA AAGATGGAAA CCGTCATACA
    AGAGAGACTC ACTTCAATAA TTTGTATAGT ATGGTGCATT ACTGTGAAAA TATAACAGAA
    TGCAGGAGAA TACAGCTTTT GGCTTACTTT GGCGAAAGTG GATTTAATCC TGATTTTTGT
    AAGAAATACC CTGATGTTTC TTGTGATAAT TGCTGTAAAA CAAAGGATTA TAAGACAAGA
    GATGTGACTG ATGATGTAAA AAATATTGTA AGATTTGTTC AAGAACATAG TTCATCACAA
    GGAACAAGAA TTAAAAACCA ATTAGGTCCT TCTGGGAGAT TTACTATGAA TATGCTAGTT
    GACATTTTCT TGGGGAGTAA GAGTGCAAAA ATTCAGTCGG GGATATTTGG ACAGGGATCT
    ACTTATTCAC GACACAATGC CGAAAGACTT TTTAAGAAAT TGATACTTGA CAAGATTTTG
    GATGAAGACT TATATATCAA TGCCAATGAC CAACCAATTG CGTATGTGAT GCCAGGAAAT
    AAAGCCCAAA CTGTACTAAA TGGGCATTTA AAGACTTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272660.1
    CDS104..3685
    Translation

    Target ORF information:

    RefSeq Version XM_022416952.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Bloom syndrome RecQ like helicase (BLM), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416952.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCTGCCG TTCCCCAGAA TAATCTACAG GAGCAACTAG AACGCCATTC AGCCAGAAAA 
    CTTAGTAACA AATTAAGTCC TTCCAAACCG AAATCTTCAG TTTTCACTTT TAAGAAGAAA
    ACATCAGCCA ACAATGTGTC TGTCACTAGT GTGTCAGTAG CAAAAACACC TGTGTTAAAT
    GACAAAGATG TTAATGTCAC TGAGGCCTTT CCCTTCAGTG AACCTCTGCC CCACGCCACA
    AGTCAGCAGA CAAGGCCCAG TGACCTCAAA AATACTCCAG CAGGACAGCA AACCAGGAGA
    ACTGTTTCAA AACCATTATT GCCAGATTTG TCACTGGTTT CACGAGTTTT ATGTAATACC
    CAGAACACAC CGATTGTAAA GAAATCCAGT AATGCTACTT TCAAGAAATT GGAATTTAGC
    TCTTCATCAG ATTCTTTTGT TACCATCAAT GATTGGGATG ATATGGATGA CTTTGATACT
    TCTGGAAATT CAAAGCTTGT TACACCATGC AAAAATCACT TTGTGAGAGT TAGCACTGCT
    CAGAAGTCAA AAAAACCTAA GAGAAACTTT TTGAAAGCAC AGCTTCCTCA AGCAAATACA
    GTAAAGGCTG ATTTGACTCC ATCCTCCTTT GAAAGTAAGC AAGCATGTTT GACTAAAAAA
    CAGAACGACG ACTCGGAATG GTTTAGTAAT GATGTGATTT GCATTGATGA TGACCCCAAT
    CCTGAAGAGC TTATACATGG AGATCCTCAG GAGAGTCATT CTTTAAAAAC TCATTTGGAA
    GAGGAAAGAG ATAGCGAAAA GAAGAACTGG GAAGAAACAG AGTTATGTTC ATTGGAGAAG
    TCGCCATGTG TAGAGCTTGA CGAAGATGAT TACGATACAG ATTTTGTTCC ACCTTCTCCA
    GAAGTAATTT CTGCCTCTTC TTCCTCTTTA AAGTGTTTTA GTATGTTAAA GGACCTTGAT
    GCCTCTGGCA CAGAGGATGG TCCTAGCACA TCCGAGGACT TGCCAACACC CAAGGGGATG
    ACAACACAGC AGCCCAATCA AGAGACCAGC ACAAACTGTG ACGGTACAAA AACTAGACAG
    CCAAGTTTAC AGCAGCAGCT TATTCACGTG ATGGACCAGA TCTGTAAATT AGTAGATACT
    ATTCCTGATG ATGAACTGAA AGCTCTGACT TGTGGGAATG CACTGCATCA GCAGCGGGAC
    ATAAGAAGGA AGCTCCTAGC TGAAGCCGAC TTTAATACAA GTGATGCCAG TGTTCTTGCT
    TCGGTGTGGA CACGCGGGCC TGATTCACTG GGAAGCCCTG TGGAGGATAT GGCATCTCTT
    TCTGCTCCTG ATGTGTTTCC AAAGGGCAAT TCCCATCCTA CCGGGAATTC TGTTAAGGAG
    TTAAATTTTC CACACCTTCC CTCACATTCC AGTTCCACTG GGGAGTGTTT ACTGGCTACT
    ACCCCAGGAA AGACAGGATT CTCCGGCACC ATGAAGAATC CCTCTGAAAG ACTGTTATTC
    AGTCCCCATT TACAGAAGTC CTTTGTAAGT AGCAACTGGG CTGAAACACC ACGGATAGAA
    AAAAGGAACG AGAGCTCTTA CTTCCTGGGA AATGTTCTCA CAAGCACGGC TGTGAGAGAT
    CAGAATAAAC GTGCTGCTCC AATAAATGAC TTAGAAATTC AGGCTGCCTG TGATATTGAT
    AATTTTGACA TAGATGGCTT TGATGATGAT GATGACTGGG AAGATATAAT GCATGATTTA
    GCGGCCAGCA GATCTTCCAC AGCTGCCTGT CTACCTATTA AGGAAGGTCG GCCAGTTAAG
    CCAGTATCAG AAAGAATTTC TTCAGCCAAG ACAAACTGTC TTCCAGTGGC ATCTACTGCT
    CAAAATAAAA ACTCAGAGTC AATTCAGAAT TACACTGACA AGTCGGCGCA AAATTTAGCA
    TCCAGAAATC TGAAACATGA ACGTTTCCAA AGTCTTAGTT TTCCTCATAC AAAAGAAATG
    ATGAAGATTT TTCATAAAAA ATTTGGCCTG CATAATTTTA GAACTAATCA GCTAGAGGCG
    ATCAATGCTG CACTGCTGGG TGAAGACTGT TTTATCTTAA TGCCCACTGG AGGTGGTAAG
    AGTCTGTGTT ACCAGCTCCC TGCCTGTGTC TCTCCTGGTG TCACTATTGT CATTTCTCCT
    TTGAGATCAC TTATAGTAGA TCAAGTCCAA AAGCTGACTT CCTTGGATAT TCCAGCTACA
    TATCTGACAG GTGATAAGAC TGACTCGGAA GCAACAAGTA TTTACCTGCA ATTATCCAAG
    AAAGACCCAA TTATAAAACT TCTATATGTT ACTCCAGAGA AGGTCTGTGC AAGTAATAGA
    CTGCTTTCCA CTCTGGAGAA TCTGTATGAG AGGAAGCTCC TGGCACGTTT TGTCATTGAT
    GAAGCGCATT GTGTCAGTCA GTGGGGACAT GATTTTCGCC AAGATTATAA AAGAATGAAT
    ATGCTTCGCC AGAAGTTTCC CTCTGTTCCA GTGATGGCCC TTACTGCCAC AGCTAATCCC
    AGGGTACAGA AGGACATCCT CACTCAGCTG AAGATCCTCC AGCCTCAGGT GTTTAGCATG
    AGCTTTAACA GACATAATCT GAAATACTAC GTGTTACCAA AAAAACCCAA GAAGGTGGCA
    TTTGATTGCT TAGAATGGAT CAGAAAGTAT CACCCACATG ACTCCGGGAT AATCTATTGC
    CTCTCCAGAC GAGAATGTGA TACAATGGCT GACACTTTAC AGAAAGATGG TCTTGCTGCT
    CTTGCTTATC ATGCTGGCCT CAGTGATTCT GCAAGGGATG AAGTACAGCA CAAGTGGATT
    AATCAGGATG GCTGCCAGGT CATCTGTGCA ACAATAGCGT TCGGAATGGG GATTGACAAA
    CCTGATGTGC GATTCGTGAT TCATGCATCT CTCCCTAAGT CTGTGGAGGG CTACTACCAA
    GAATCTGGCA GAGCTGGAAG AGATGGGGAA ATTTCTCACT GCCTGCTGTT CTATACCTAT
    CATGATGTGA CACGACTCAA GAGACTCATC CTGATGGAAA AAGATGGAAA CCGTCATACA
    AGAGAGACTC ACTTCAATAA TTTGTATAGT ATGGTGCATT ACTGTGAAAA TATAACAGAA
    TGCAGGAGAA TACAGCTTTT GGCTTACTTT GGCGAAAGTG GATTTAATCC TGATTTTTGT
    AAGAAATACC CTGATGTTTC TTGTGATAAT TGCTGTAAAA CAAAGGATTA TAAGACAAGA
    GATGTGACTG ATGATGTAAA AAATATTGTA AGATTTGTTC AAGAACATAG TTCATCACAA
    GGAACAAGAA TTAAAAACCA ATTAGGTCCT TCTGGGAGAT TTACTATGAA TATGCTAGTT
    GACATTTTCT TGGGGAGTAA GAGTGCAAAA ATTCAGTCGG GGATATTTGG ACAGGGATCT
    ACTTATTCAC GACACAATGC CGAAAGACTT TTTAAGAAAT TGATACTTGA CAAGATTTTG
    GATGAAGACT TATATATCAA TGCCAATGAC CAACCAATTG CGTATGTGAT GCCAGGAAAT
    AAAGCCCAAA CTGTACTAAA TGGGCATTTA AAGACTTTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35734
    Clone ID Related Accession (Same CDS sequence) XM_014112440.1
    Accession Version XM_014112440.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product Bloom syndrome protein isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTGCCG TTCCCCAGAA TAATCTACAG GAGCAACTAG AACGCCATTC AGCCAGAAAA 
    CTTAGTAACA AATTAAGTCC TTCCAAACCG AAATCTTCAG TTTTCACTTT TAAGAAGAAA
    ACATCAGCCA ACAATGTGTC TGTCACTAGT GTGTCAGTAG CAAAAACACC TGTGTTAAAT
    GACAAAGATG TTAATGTCAC TGAGGCCTTT CCCTTCAGTG AACCTCTGCC CCACGCCACA
    AGTCAGCAGA CAAGGCCCAG TGACCTCAAA AATACTCCAG CAGGACAGCA AACCAGGAGA
    ACTGTTTCAA AACCATTATT GCCAGATTTG TCACTGGTTT CACGAGTTTT ATGTAATACC
    CAGAACACAC CGATTGTAAA GAAATCCAGT AATGCTACTT TCAAGAAATT GGAATTTAGC
    TCTTCATCAG ATTCTTTTGT TACCATCAAT GATTGGGATG ATATGGATGA CTTTGATACT
    TCTGGAAATT CAAAGCTTGT TACACCATGC AAAAATCACT TTGTGAGAGT TAGCACTGCT
    CAGAAGTCAA AAAAACCTAA GAGAAACTTT TTGAAAGCAC AGCTTCCTCA AGCAAATACA
    GTAAAGGCTG ATTTGACTCC ATCCTCCTTT GAAAGTAAGC AAGCATGTTT GACTAAAAAA
    CAGAACGACG ACTCGGAATG GTTTAGTAAT GATGTGATTT GCATTGATGA TGACCCCAAT
    CCTGAAGAGC TTATACATGG AGATCCTCAG GAGAGTCATT CTTTAAAAAC TCATTTGGAA
    GAGGAAAGAG ATAGCGAAAA GAAGAACTGG GAAGAAACAG AGTTATGTTC ATTGGAGAAG
    TCGCCATGTG TAGAGCTTGA CGAAGATGAT TACGATACAG ATTTTGTTCC ACCTTCTCCA
    GAAGTAATTT CTGCCTCTTC TTCCTCTTTA AAGTGTTTTA GACAGCCAAG TTTACAGCAG
    CAGCTTATTC ACGTGATGGA CCAGATCTGT AAATTAGTAG ATACTATTCC TGATGATGAA
    CTGAAAGCTC TGACTTGTGG GAATGCACTG CATCAGCAGC GGGACATAAG AAGGAAGCTC
    CTAGCTGAAG CCGACTTTAA TACAAGTGAT GCCAGTGTTC TTGCTTCGGT GTGGACACGC
    GGGCCTGATT CACTGGGAAG CCCTGTGGAG GATATGGCAT CTCTTTCTGC TCCTGATGTG
    TTTCCAAAGG GCAATTCCCA TCCTACCGGG AATTCTGTTA AGGAGTTAAA TTTTCCACAC
    CTTCCCTCAC ATTCCAGTTC CACTGGGGAG TGTTTACTGG CTACTACCCC AGGAAAGACA
    GGATTCTCCG GCACCATGAA GAATCCCTCT GAAAGACTGT TATTCAGTCC CCATTTACAG
    AAGTCCTTTG TAAGTAGCAA CTGGGCTGAA ACACCACGGA TAGAAAAAAG GAACGAGAGC
    TCTTACTTCC TGGGAAATGT TCTCACAAGC ACGGCTGTGA GAGATCAGAA TAAACGTGCT
    GCTCCAATAA ATGACTTAGA AATTCAGGCT GCCTGTGATA TTGATAATTT TGACATAGAT
    GGCTTTGATG ATGATGATGA CTGGGAAGAT ATAATGCATG ATTTAGCGGC CAGCAGATCT
    TCCACAGCTG CCTGTCTACC TATTAAGGAA GGTCGGCCAG TTAAGCCAGT ATCAGAAAGA
    ATTTCTTCAG CCAAGACAAA CTGTCTTCCA GTGGCATCTA CTGCTCAAAA TAAAAACTCA
    GAGTCAATTC AGAATTACAC TGACAAGTCG GCGCAAAATT TAGCATCCAG AAATCTGAAA
    CATGAACGTT TCCAAAGTCT TAGTTTTCCT CATACAAAAG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTGGGTGAAG ACTGTTTTAT CTTAATGCCC ACTGGAGGTG GTAAGAGTCT GTGTTACCAG
    CTCCCTGCCT GTGTCTCTCC TGGTGTCACT ATTGTCATTT CTCCTTTGAG ATCACTTATA
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CGGAAGCAAC AAGTATTTAC CTGCAATTAT CCAAGAAAGA CCCAATTATA
    AAACTTCTAT ATGTTACTCC AGAGAAGGTC TGTGCAAGTA ATAGACTGCT TTCCACTCTG
    GAGAATCTGT ATGAGAGGAA GCTCCTGGCA CGTTTTGTCA TTGATGAAGC GCATTGTGTC
    AGTCAGTGGG GACATGATTT TCGCCAAGAT TATAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCCTCTG TTCCAGTGAT GGCCCTTACT GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTCACTC AGCTGAAGAT CCTCCAGCCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
    AATCTGAAAT ACTACGTGTT ACCAAAAAAA CCCAAGAAGG TGGCATTTGA TTGCTTAGAA
    TGGATCAGAA AGTATCACCC ACATGACTCC GGGATAATCT ATTGCCTCTC CAGACGAGAA
    TGTGATACAA TGGCTGACAC TTTACAGAAA GATGGTCTTG CTGCTCTTGC TTATCATGCT
    GGCCTCAGTG ATTCTGCAAG GGATGAAGTA CAGCACAAGT GGATTAATCA GGATGGCTGC
    CAGGTCATCT GTGCAACAAT AGCGTTCGGA ATGGGGATTG ACAAACCTGA TGTGCGATTC
    GTGATTCATG CATCTCTCCC TAAGTCTGTG GAGGGCTACT ACCAAGAATC TGGCAGAGCT
    GGAAGAGATG GGGAAATTTC TCACTGCCTG CTGTTCTATA CCTATCATGA TGTGACACGA
    CTCAAGAGAC TCATCCTGAT GGAAAAAGAT GGAAACCGTC ATACAAGAGA GACTCACTTC
    AATAATTTGT ATAGTATGGT GCATTACTGT GAAAATATAA CAGAATGCAG GAGAATACAG
    CTTTTGGCTT ACTTTGGCGA AAGTGGATTT AATCCTGATT TTTGTAAGAA ATACCCTGAT
    GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAGA CAAGAGATGT GACTGATGAT
    GTAAAAAATA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAC AAGAATTAAA
    AACCAATTAG GTCCTTCTGG GAGATTTACT ATGAATATGC TAGTTGACAT TTTCTTGGGG
    AGTAAGAGTG CAAAAATTCA GTCGGGGATA TTTGGACAGG GATCTACTTA TTCACGACAC
    AATGCCGAAA GACTTTTTAA GAAATTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGACCAACC AATTGCGTAT GTGATGCCAG GAAATAAAGC CCAAACTGTA
    CTAAATGGGC ATTTAAAGGT AGACTTTATA GAAACGGAAA ATTCTACCAG TATAAAAAAA
    CAGAAAGCTG CTTTAGTGGC AAAGATGTCT CAGAGAGAAG AAGTGGTAAA GAAGTGTCTT
    GGAGAACTGA CAGAAGTGTG TAAATCTCTG GGAAAAGTTT TTGGTGTCCA CTACTTTAAT
    ATTTTTAATA CTGTGACTCT CAAGAAGCTT GCAGAATCTC TATCTTCTGA TCCCGAGGTT
    TTGCTTCAAA TTGATGGTGT TACTGAAGAC AAACTGGAAA AATATGGTGC AGAAGTGATT
    CCAATATTAC AGAAATACTC TGAGTGGACA TTGCCAGCTG ACGATGGTTC CCCACGAATC
    AGCCCGAGCA GCAGCAGAAG CACCAGGAGA AGTGCCCCTG AGGAGTTCGA CGAGGAAACA
    TCCGTATCTT CTCACTACTT TGCAAATAAA ACCAAAAATG AAAGAAAGAG GAAAAGGATG
    TCAGCCACCC AGAGGTCTAA GAGGAGGAGA ACTGGCTTTG GTGGCTCCAA GACAAAAGGG
    GGGTCCACCG CATGCAGAAA GACATCTTCT AAAAGTAAAT CCTCCAACAT ATTTGGACCT
    CATTCATCTT TGCATGGTTC TCAAGCAGCA TCAGGAGCCG CTAGAAAATT GGGGATTATG
    GCTCCCCCAA AGCCTATAAA TAGACCGTTT CTTAAGCCTT CATATGCATT TTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967915.1
    CDS77..4213
    Translation

    Target ORF information:

    RefSeq Version XM_014112440.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Bloom syndrome, RecQ helicase-like (BLM), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112440.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTGCCG TTCCCCAGAA TAATCTACAG GAGCAACTAG AACGCCATTC AGCCAGAAAA 
    CTTAGTAACA AATTAAGTCC TTCCAAACCG AAATCTTCAG TTTTCACTTT TAAGAAGAAA
    ACATCAGCCA ACAATGTGTC TGTCACTAGT GTGTCAGTAG CAAAAACACC TGTGTTAAAT
    GACAAAGATG TTAATGTCAC TGAGGCCTTT CCCTTCAGTG AACCTCTGCC CCACGCCACA
    AGTCAGCAGA CAAGGCCCAG TGACCTCAAA AATACTCCAG CAGGACAGCA AACCAGGAGA
    ACTGTTTCAA AACCATTATT GCCAGATTTG TCACTGGTTT CACGAGTTTT ATGTAATACC
    CAGAACACAC CGATTGTAAA GAAATCCAGT AATGCTACTT TCAAGAAATT GGAATTTAGC
    TCTTCATCAG ATTCTTTTGT TACCATCAAT GATTGGGATG ATATGGATGA CTTTGATACT
    TCTGGAAATT CAAAGCTTGT TACACCATGC AAAAATCACT TTGTGAGAGT TAGCACTGCT
    CAGAAGTCAA AAAAACCTAA GAGAAACTTT TTGAAAGCAC AGCTTCCTCA AGCAAATACA
    GTAAAGGCTG ATTTGACTCC ATCCTCCTTT GAAAGTAAGC AAGCATGTTT GACTAAAAAA
    CAGAACGACG ACTCGGAATG GTTTAGTAAT GATGTGATTT GCATTGATGA TGACCCCAAT
    CCTGAAGAGC TTATACATGG AGATCCTCAG GAGAGTCATT CTTTAAAAAC TCATTTGGAA
    GAGGAAAGAG ATAGCGAAAA GAAGAACTGG GAAGAAACAG AGTTATGTTC ATTGGAGAAG
    TCGCCATGTG TAGAGCTTGA CGAAGATGAT TACGATACAG ATTTTGTTCC ACCTTCTCCA
    GAAGTAATTT CTGCCTCTTC TTCCTCTTTA AAGTGTTTTA GACAGCCAAG TTTACAGCAG
    CAGCTTATTC ACGTGATGGA CCAGATCTGT AAATTAGTAG ATACTATTCC TGATGATGAA
    CTGAAAGCTC TGACTTGTGG GAATGCACTG CATCAGCAGC GGGACATAAG AAGGAAGCTC
    CTAGCTGAAG CCGACTTTAA TACAAGTGAT GCCAGTGTTC TTGCTTCGGT GTGGACACGC
    GGGCCTGATT CACTGGGAAG CCCTGTGGAG GATATGGCAT CTCTTTCTGC TCCTGATGTG
    TTTCCAAAGG GCAATTCCCA TCCTACCGGG AATTCTGTTA AGGAGTTAAA TTTTCCACAC
    CTTCCCTCAC ATTCCAGTTC CACTGGGGAG TGTTTACTGG CTACTACCCC AGGAAAGACA
    GGATTCTCCG GCACCATGAA GAATCCCTCT GAAAGACTGT TATTCAGTCC CCATTTACAG
    AAGTCCTTTG TAAGTAGCAA CTGGGCTGAA ACACCACGGA TAGAAAAAAG GAACGAGAGC
    TCTTACTTCC TGGGAAATGT TCTCACAAGC ACGGCTGTGA GAGATCAGAA TAAACGTGCT
    GCTCCAATAA ATGACTTAGA AATTCAGGCT GCCTGTGATA TTGATAATTT TGACATAGAT
    GGCTTTGATG ATGATGATGA CTGGGAAGAT ATAATGCATG ATTTAGCGGC CAGCAGATCT
    TCCACAGCTG CCTGTCTACC TATTAAGGAA GGTCGGCCAG TTAAGCCAGT ATCAGAAAGA
    ATTTCTTCAG CCAAGACAAA CTGTCTTCCA GTGGCATCTA CTGCTCAAAA TAAAAACTCA
    GAGTCAATTC AGAATTACAC TGACAAGTCG GCGCAAAATT TAGCATCCAG AAATCTGAAA
    CATGAACGTT TCCAAAGTCT TAGTTTTCCT CATACAAAAG AAATGATGAA GATTTTTCAT
    AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
    CTGGGTGAAG ACTGTTTTAT CTTAATGCCC ACTGGAGGTG GTAAGAGTCT GTGTTACCAG
    CTCCCTGCCT GTGTCTCTCC TGGTGTCACT ATTGTCATTT CTCCTTTGAG ATCACTTATA
    GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
    AAGACTGACT CGGAAGCAAC AAGTATTTAC CTGCAATTAT CCAAGAAAGA CCCAATTATA
    AAACTTCTAT ATGTTACTCC AGAGAAGGTC TGTGCAAGTA ATAGACTGCT TTCCACTCTG
    GAGAATCTGT ATGAGAGGAA GCTCCTGGCA CGTTTTGTCA TTGATGAAGC GCATTGTGTC
    AGTCAGTGGG GACATGATTT TCGCCAAGAT TATAAAAGAA TGAATATGCT TCGCCAGAAG
    TTTCCCTCTG TTCCAGTGAT GGCCCTTACT GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
    ATCCTCACTC AGCTGAAGAT CCTCCAGCCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
    AATCTGAAAT ACTACGTGTT ACCAAAAAAA CCCAAGAAGG TGGCATTTGA TTGCTTAGAA
    TGGATCAGAA AGTATCACCC ACATGACTCC GGGATAATCT ATTGCCTCTC CAGACGAGAA
    TGTGATACAA TGGCTGACAC TTTACAGAAA GATGGTCTTG CTGCTCTTGC TTATCATGCT
    GGCCTCAGTG ATTCTGCAAG GGATGAAGTA CAGCACAAGT GGATTAATCA GGATGGCTGC
    CAGGTCATCT GTGCAACAAT AGCGTTCGGA ATGGGGATTG ACAAACCTGA TGTGCGATTC
    GTGATTCATG CATCTCTCCC TAAGTCTGTG GAGGGCTACT ACCAAGAATC TGGCAGAGCT
    GGAAGAGATG GGGAAATTTC TCACTGCCTG CTGTTCTATA CCTATCATGA TGTGACACGA
    CTCAAGAGAC TCATCCTGAT GGAAAAAGAT GGAAACCGTC ATACAAGAGA GACTCACTTC
    AATAATTTGT ATAGTATGGT GCATTACTGT GAAAATATAA CAGAATGCAG GAGAATACAG
    CTTTTGGCTT ACTTTGGCGA AAGTGGATTT AATCCTGATT TTTGTAAGAA ATACCCTGAT
    GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAGA CAAGAGATGT GACTGATGAT
    GTAAAAAATA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAC AAGAATTAAA
    AACCAATTAG GTCCTTCTGG GAGATTTACT ATGAATATGC TAGTTGACAT TTTCTTGGGG
    AGTAAGAGTG CAAAAATTCA GTCGGGGATA TTTGGACAGG GATCTACTTA TTCACGACAC
    AATGCCGAAA GACTTTTTAA GAAATTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT
    ATCAATGCCA ATGACCAACC AATTGCGTAT GTGATGCCAG GAAATAAAGC CCAAACTGTA
    CTAAATGGGC ATTTAAAGGT AGACTTTATA GAAACGGAAA ATTCTACCAG TATAAAAAAA
    CAGAAAGCTG CTTTAGTGGC AAAGATGTCT CAGAGAGAAG AAGTGGTAAA GAAGTGTCTT
    GGAGAACTGA CAGAAGTGTG TAAATCTCTG GGAAAAGTTT TTGGTGTCCA CTACTTTAAT
    ATTTTTAATA CTGTGACTCT CAAGAAGCTT GCAGAATCTC TATCTTCTGA TCCCGAGGTT
    TTGCTTCAAA TTGATGGTGT TACTGAAGAC AAACTGGAAA AATATGGTGC AGAAGTGATT
    CCAATATTAC AGAAATACTC TGAGTGGACA TTGCCAGCTG ACGATGGTTC CCCACGAATC
    AGCCCGAGCA GCAGCAGAAG CACCAGGAGA AGTGCCCCTG AGGAGTTCGA CGAGGAAACA
    TCCGTATCTT CTCACTACTT TGCAAATAAA ACCAAAAATG AAAGAAAGAG GAAAAGGATG
    TCAGCCACCC AGAGGTCTAA GAGGAGGAGA ACTGGCTTTG GTGGCTCCAA GACAAAAGGG
    GGGTCCACCG CATGCAGAAA GACATCTTCT AAAAGTAAAT CCTCCAACAT ATTTGGACCT
    CATTCATCTT TGCATGGTTC TCAAGCAGCA TCAGGAGCCG CTAGAAAATT GGGGATTATG
    GCTCCCCCAA AGCCTATAAA TAGACCGTTT CTTAAGCCTT CATATGCATT TTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35735
    Clone ID Related Accession (Same CDS sequence) XM_014112441.1
    Accession Version XM_014112441.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product Bloom syndrome protein isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCTGCCG TTCCCCAGAA TAATCTACAG GAGCAACTAG AACGCCATTC AGCCAGAAAA 
    CTTAGTAACA AATTAAGTCC TTCCAAACCG AAATCTTCAG TTTTCACTTT TAAGAAGAAA
    ACATCAGCCA ACAATGTGTC TGTCACTAGT GTGTCAGTAG CAAAAACACC TGTGTTAAAT
    GACAAAGATG TTAATGTCAC TGAGGCCTTT CCCTTCAGTG AACCTCTGCC CCACGCCACA
    AGTCAGCAGA CAAGGCCCAG TGACCTCAAA AATACTCCAG CAGGACAGCA AACCAGGAGA
    ACTGTTTCAA AACCATTATT GCCAGATTTG TCACTGGTTT CACGAGTTTT ATGTAATACC
    CAGAACACAC CGATTGTAAA GAAATCCAGT AATGCTACTT TCAAGAAATT GGAATTTAGC
    TCTTCATCAG ATTCTTTTGT TACCATCAAT GATTGGGATG ATATGGATGA CTTTGATACT
    TCTGGAAATT CAAAGCTTGT TACACCATGC AAAAATCACT TTGTGAGAGT TAGCACTGCT
    CAGAAGTCAA AAAAACCTAA GAGAAACTTT TTGAAAGCAC AGCTTCCTCA AGCAAATACA
    GTAAAGGCTG ATTTGACTCC ATCCTCCTTT GAAAGTAAGC AAGCATGTTT GACTAAAAAA
    CAGAACGACG ACTCGGAATG GTTTAGTAAT GATGTGATTT GCATTGATGA TGACCCCAAT
    CCTGAAGAGC TTATACATGG AGATCCTCAG GAGAGTCATT CTTTAAAAAC TCATTTGGAA
    GAGGAAAGAG CTAGACAGCC AAGTTTACAG CAGCAGCTTA TTCACGTGAT GGACCAGATC
    TGTAAATTAG TAGATACTAT TCCTGATGAT GAACTGAAAG CTCTGACTTG TGGGAATGCA
    CTGCATCAGC AGCGGGACAT AAGAAGGAAG CTCCTAGCTG AAGCCGACTT TAATACAAGT
    GATGCCAGTG TTCTTGCTTC GGTGTGGACA CGCGGGCCTG ATTCACTGGG AAGCCCTGTG
    GAGGATATGG CATCTCTTTC TGCTCCTGAT GTGTTTCCAA AGGGCAATTC CCATCCTACC
    GGGAATTCTG TTAAGGAGTT AAATTTTCCA CACCTTCCCT CACATTCCAG TTCCACTGGG
    GAGTGTTTAC TGGCTACTAC CCCAGGAAAG ACAGGATTCT CCGGCACCAT GAAGAATCCC
    TCTGAAAGAC TGTTATTCAG TCCCCATTTA CAGAAGTCCT TTGTAAGTAG CAACTGGGCT
    GAAACACCAC GGATAGAAAA AAGGAACGAG AGCTCTTACT TCCTGGGAAA TGTTCTCACA
    AGCACGGCTG TGAGAGATCA GAATAAACGT GCTGCTCCAA TAAATGACTT AGAAATTCAG
    GCTGCCTGTG ATATTGATAA TTTTGACATA GATGGCTTTG ATGATGATGA TGACTGGGAA
    GATATAATGC ATGATTTAGC GGCCAGCAGA TCTTCCACAG CTGCCTGTCT ACCTATTAAG
    GAAGGTCGGC CAGTTAAGCC AGTATCAGAA AGAATTTCTT CAGCCAAGAC AAACTGTCTT
    CCAGTGGCAT CTACTGCTCA AAATAAAAAC TCAGAGTCAA TTCAGAATTA CACTGACAAG
    TCGGCGCAAA ATTTAGCATC CAGAAATCTG AAACATGAAC GTTTCCAAAG TCTTAGTTTT
    CCTCATACAA AAGAAATGAT GAAGATTTTT CATAAAAAAT TTGGCCTGCA TAATTTTAGA
    ACTAATCAGC TAGAGGCGAT CAATGCTGCA CTGCTGGGTG AAGACTGTTT TATCTTAATG
    CCCACTGGAG GTGGTAAGAG TCTGTGTTAC CAGCTCCCTG CCTGTGTCTC TCCTGGTGTC
    ACTATTGTCA TTTCTCCTTT GAGATCACTT ATAGTAGATC AAGTCCAAAA GCTGACTTCC
    TTGGATATTC CAGCTACATA TCTGACAGGT GATAAGACTG ACTCGGAAGC AACAAGTATT
    TACCTGCAAT TATCCAAGAA AGACCCAATT ATAAAACTTC TATATGTTAC TCCAGAGAAG
    GTCTGTGCAA GTAATAGACT GCTTTCCACT CTGGAGAATC TGTATGAGAG GAAGCTCCTG
    GCACGTTTTG TCATTGATGA AGCGCATTGT GTCAGTCAGT GGGGACATGA TTTTCGCCAA
    GATTATAAAA GAATGAATAT GCTTCGCCAG AAGTTTCCCT CTGTTCCAGT GATGGCCCTT
    ACTGCCACAG CTAATCCCAG GGTACAGAAG GACATCCTCA CTCAGCTGAA GATCCTCCAG
    CCTCAGGTGT TTAGCATGAG CTTTAACAGA CATAATCTGA AATACTACGT GTTACCAAAA
    AAACCCAAGA AGGTGGCATT TGATTGCTTA GAATGGATCA GAAAGTATCA CCCACATGAC
    TCCGGGATAA TCTATTGCCT CTCCAGACGA GAATGTGATA CAATGGCTGA CACTTTACAG
    AAAGATGGTC TTGCTGCTCT TGCTTATCAT GCTGGCCTCA GTGATTCTGC AAGGGATGAA
    GTACAGCACA AGTGGATTAA TCAGGATGGC TGCCAGGTCA TCTGTGCAAC AATAGCGTTC
    GGAATGGGGA TTGACAAACC TGATGTGCGA TTCGTGATTC ATGCATCTCT CCCTAAGTCT
    GTGGAGGGCT ACTACCAAGA ATCTGGCAGA GCTGGAAGAG ATGGGGAAAT TTCTCACTGC
    CTGCTGTTCT ATACCTATCA TGATGTGACA CGACTCAAGA GACTCATCCT GATGGAAAAA
    GATGGAAACC GTCATACAAG AGAGACTCAC TTCAATAATT TGTATAGTAT GGTGCATTAC
    TGTGAAAATA TAACAGAATG CAGGAGAATA CAGCTTTTGG CTTACTTTGG CGAAAGTGGA
    TTTAATCCTG ATTTTTGTAA GAAATACCCT GATGTTTCTT GTGATAATTG CTGTAAAACA
    AAGGATTATA AGACAAGAGA TGTGACTGAT GATGTAAAAA ATATTGTAAG ATTTGTTCAA
    GAACATAGTT CATCACAAGG AACAAGAATT AAAAACCAAT TAGGTCCTTC TGGGAGATTT
    ACTATGAATA TGCTAGTTGA CATTTTCTTG GGGAGTAAGA GTGCAAAAAT TCAGTCGGGG
    ATATTTGGAC AGGGATCTAC TTATTCACGA CACAATGCCG AAAGACTTTT TAAGAAATTG
    ATACTTGACA AGATTTTGGA TGAAGACTTA TATATCAATG CCAATGACCA ACCAATTGCG
    TATGTGATGC CAGGAAATAA AGCCCAAACT GTACTAAATG GGCATTTAAA GGTAGACTTT
    ATAGAAACGG AAAATTCTAC CAGTATAAAA AAACAGAAAG CTGCTTTAGT GGCAAAGATG
    TCTCAGAGAG AAGAAGTGGT AAAGAAGTGT CTTGGAGAAC TGACAGAAGT GTGTAAATCT
    CTGGGAAAAG TTTTTGGTGT CCACTACTTT AATATTTTTA ATACTGTGAC TCTCAAGAAG
    CTTGCAGAAT CTCTATCTTC TGATCCCGAG GTTTTGCTTC AAATTGATGG TGTTACTGAA
    GACAAACTGG AAAAATATGG TGCAGAAGTG ATTCCAATAT TACAGAAATA CTCTGAGTGG
    ACATTGCCAG CTGACGATGG TTCCCCACGA ATCAGCCCGA GCAGCAGCAG AAGCACCAGG
    AGAAGTGCCC CTGAGGAGTT CGACGAGGAA ACATCCGTAT CTTCTCACTA CTTTGCAAAT
    AAAACCAAAA ATGAAAGAAA GAGGAAAAGG ATGTCAGCCA CCCAGAGGTC TAAGAGGAGG
    AGAACTGGCT TTGGTGGCTC CAAGACAAAA GGGGGGTCCA CCGCATGCAG AAAGACATCT
    TCTAAAAGTA AATCCTCCAA CATATTTGGA CCTCATTCAT CTTTGCATGG TTCTCAAGCA
    GCATCAGGAG CCGCTAGAAA ATTGGGGATT ATGGCTCCCC CAAAGCCTAT AAATAGACCG
    TTTCTTAAGC CTTCATATGC ATTTTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967916.1
    CDS77..4066
    Translation

    Target ORF information:

    RefSeq Version XM_014112441.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Bloom syndrome, RecQ helicase-like (BLM), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112441.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCTGCCG TTCCCCAGAA TAATCTACAG GAGCAACTAG AACGCCATTC AGCCAGAAAA 
    CTTAGTAACA AATTAAGTCC TTCCAAACCG AAATCTTCAG TTTTCACTTT TAAGAAGAAA
    ACATCAGCCA ACAATGTGTC TGTCACTAGT GTGTCAGTAG CAAAAACACC TGTGTTAAAT
    GACAAAGATG TTAATGTCAC TGAGGCCTTT CCCTTCAGTG AACCTCTGCC CCACGCCACA
    AGTCAGCAGA CAAGGCCCAG TGACCTCAAA AATACTCCAG CAGGACAGCA AACCAGGAGA
    ACTGTTTCAA AACCATTATT GCCAGATTTG TCACTGGTTT CACGAGTTTT ATGTAATACC
    CAGAACACAC CGATTGTAAA GAAATCCAGT AATGCTACTT TCAAGAAATT GGAATTTAGC
    TCTTCATCAG ATTCTTTTGT TACCATCAAT GATTGGGATG ATATGGATGA CTTTGATACT
    TCTGGAAATT CAAAGCTTGT TACACCATGC AAAAATCACT TTGTGAGAGT TAGCACTGCT
    CAGAAGTCAA AAAAACCTAA GAGAAACTTT TTGAAAGCAC AGCTTCCTCA AGCAAATACA
    GTAAAGGCTG ATTTGACTCC ATCCTCCTTT GAAAGTAAGC AAGCATGTTT GACTAAAAAA
    CAGAACGACG ACTCGGAATG GTTTAGTAAT GATGTGATTT GCATTGATGA TGACCCCAAT
    CCTGAAGAGC TTATACATGG AGATCCTCAG GAGAGTCATT CTTTAAAAAC TCATTTGGAA
    GAGGAAAGAG CTAGACAGCC AAGTTTACAG CAGCAGCTTA TTCACGTGAT GGACCAGATC
    TGTAAATTAG TAGATACTAT TCCTGATGAT GAACTGAAAG CTCTGACTTG TGGGAATGCA
    CTGCATCAGC AGCGGGACAT AAGAAGGAAG CTCCTAGCTG AAGCCGACTT TAATACAAGT
    GATGCCAGTG TTCTTGCTTC GGTGTGGACA CGCGGGCCTG ATTCACTGGG AAGCCCTGTG
    GAGGATATGG CATCTCTTTC TGCTCCTGAT GTGTTTCCAA AGGGCAATTC CCATCCTACC
    GGGAATTCTG TTAAGGAGTT AAATTTTCCA CACCTTCCCT CACATTCCAG TTCCACTGGG
    GAGTGTTTAC TGGCTACTAC CCCAGGAAAG ACAGGATTCT CCGGCACCAT GAAGAATCCC
    TCTGAAAGAC TGTTATTCAG TCCCCATTTA CAGAAGTCCT TTGTAAGTAG CAACTGGGCT
    GAAACACCAC GGATAGAAAA AAGGAACGAG AGCTCTTACT TCCTGGGAAA TGTTCTCACA
    AGCACGGCTG TGAGAGATCA GAATAAACGT GCTGCTCCAA TAAATGACTT AGAAATTCAG
    GCTGCCTGTG ATATTGATAA TTTTGACATA GATGGCTTTG ATGATGATGA TGACTGGGAA
    GATATAATGC ATGATTTAGC GGCCAGCAGA TCTTCCACAG CTGCCTGTCT ACCTATTAAG
    GAAGGTCGGC CAGTTAAGCC AGTATCAGAA AGAATTTCTT CAGCCAAGAC AAACTGTCTT
    CCAGTGGCAT CTACTGCTCA AAATAAAAAC TCAGAGTCAA TTCAGAATTA CACTGACAAG
    TCGGCGCAAA ATTTAGCATC CAGAAATCTG AAACATGAAC GTTTCCAAAG TCTTAGTTTT
    CCTCATACAA AAGAAATGAT GAAGATTTTT CATAAAAAAT TTGGCCTGCA TAATTTTAGA
    ACTAATCAGC TAGAGGCGAT CAATGCTGCA CTGCTGGGTG AAGACTGTTT TATCTTAATG
    CCCACTGGAG GTGGTAAGAG TCTGTGTTAC CAGCTCCCTG CCTGTGTCTC TCCTGGTGTC
    ACTATTGTCA TTTCTCCTTT GAGATCACTT ATAGTAGATC AAGTCCAAAA GCTGACTTCC
    TTGGATATTC CAGCTACATA TCTGACAGGT GATAAGACTG ACTCGGAAGC AACAAGTATT
    TACCTGCAAT TATCCAAGAA AGACCCAATT ATAAAACTTC TATATGTTAC TCCAGAGAAG
    GTCTGTGCAA GTAATAGACT GCTTTCCACT CTGGAGAATC TGTATGAGAG GAAGCTCCTG
    GCACGTTTTG TCATTGATGA AGCGCATTGT GTCAGTCAGT GGGGACATGA TTTTCGCCAA
    GATTATAAAA GAATGAATAT GCTTCGCCAG AAGTTTCCCT CTGTTCCAGT GATGGCCCTT
    ACTGCCACAG CTAATCCCAG GGTACAGAAG GACATCCTCA CTCAGCTGAA GATCCTCCAG
    CCTCAGGTGT TTAGCATGAG CTTTAACAGA CATAATCTGA AATACTACGT GTTACCAAAA
    AAACCCAAGA AGGTGGCATT TGATTGCTTA GAATGGATCA GAAAGTATCA CCCACATGAC
    TCCGGGATAA TCTATTGCCT CTCCAGACGA GAATGTGATA CAATGGCTGA CACTTTACAG
    AAAGATGGTC TTGCTGCTCT TGCTTATCAT GCTGGCCTCA GTGATTCTGC AAGGGATGAA
    GTACAGCACA AGTGGATTAA TCAGGATGGC TGCCAGGTCA TCTGTGCAAC AATAGCGTTC
    GGAATGGGGA TTGACAAACC TGATGTGCGA TTCGTGATTC ATGCATCTCT CCCTAAGTCT
    GTGGAGGGCT ACTACCAAGA ATCTGGCAGA GCTGGAAGAG ATGGGGAAAT TTCTCACTGC
    CTGCTGTTCT ATACCTATCA TGATGTGACA CGACTCAAGA GACTCATCCT GATGGAAAAA
    GATGGAAACC GTCATACAAG AGAGACTCAC TTCAATAATT TGTATAGTAT GGTGCATTAC
    TGTGAAAATA TAACAGAATG CAGGAGAATA CAGCTTTTGG CTTACTTTGG CGAAAGTGGA
    TTTAATCCTG ATTTTTGTAA GAAATACCCT GATGTTTCTT GTGATAATTG CTGTAAAACA
    AAGGATTATA AGACAAGAGA TGTGACTGAT GATGTAAAAA ATATTGTAAG ATTTGTTCAA
    GAACATAGTT CATCACAAGG AACAAGAATT AAAAACCAAT TAGGTCCTTC TGGGAGATTT
    ACTATGAATA TGCTAGTTGA CATTTTCTTG GGGAGTAAGA GTGCAAAAAT TCAGTCGGGG
    ATATTTGGAC AGGGATCTAC TTATTCACGA CACAATGCCG AAAGACTTTT TAAGAAATTG
    ATACTTGACA AGATTTTGGA TGAAGACTTA TATATCAATG CCAATGACCA ACCAATTGCG
    TATGTGATGC CAGGAAATAA AGCCCAAACT GTACTAAATG GGCATTTAAA GGTAGACTTT
    ATAGAAACGG AAAATTCTAC CAGTATAAAA AAACAGAAAG CTGCTTTAGT GGCAAAGATG
    TCTCAGAGAG AAGAAGTGGT AAAGAAGTGT CTTGGAGAAC TGACAGAAGT GTGTAAATCT
    CTGGGAAAAG TTTTTGGTGT CCACTACTTT AATATTTTTA ATACTGTGAC TCTCAAGAAG
    CTTGCAGAAT CTCTATCTTC TGATCCCGAG GTTTTGCTTC AAATTGATGG TGTTACTGAA
    GACAAACTGG AAAAATATGG TGCAGAAGTG ATTCCAATAT TACAGAAATA CTCTGAGTGG
    ACATTGCCAG CTGACGATGG TTCCCCACGA ATCAGCCCGA GCAGCAGCAG AAGCACCAGG
    AGAAGTGCCC CTGAGGAGTT CGACGAGGAA ACATCCGTAT CTTCTCACTA CTTTGCAAAT
    AAAACCAAAA ATGAAAGAAA GAGGAAAAGG ATGTCAGCCA CCCAGAGGTC TAAGAGGAGG
    AGAACTGGCT TTGGTGGCTC CAAGACAAAA GGGGGGTCCA CCGCATGCAG AAAGACATCT
    TCTAAAAGTA AATCCTCCAA CATATTTGGA CCTCATTCAT CTTTGCATGG TTCTCAAGCA
    GCATCAGGAG CCGCTAGAAA ATTGGGGATT ATGGCTCCCC CAAAGCCTAT AAATAGACCG
    TTTCTTAAGC CTTCATATGC ATTTTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35736
    Clone ID Related Accession (Same CDS sequence) XM_014112442.1 , XM_014112442.2
    Accession Version XM_014112442.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product Bloom syndrome protein isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACCAGA TCTGTAAATT AGTAGATACT ATTCCTGATG ATGAACTGAA AGCTCTGACT 
    TGTGGGAATG CACTGCATCA GCAGCGGGAC ATAAGAAGGA AGCTCCTAGC TGAAGCCGAC
    TTTAATACAA GTGATGCCAG TGTTCTTGCT TCGGTGTGGA CACGCGGGCC TGATTCACTG
    GGAAGCCCTG TGGAGGATAT GGCATCTCTT TCTGCTCCTG ATGTGTTTCC AAAGGGCAAT
    TCCCATCCTA CCGGGAATTC TGTTAAGGAG TTAAATTTTC CACACCTTCC CTCACATTCC
    AGTTCCACTG GGGAGTGTTT ACTGGCTACT ACCCCAGGAA AGACAGGATT CTCCGGCACC
    ATGAAGAATC CCTCTGAAAG ACTGTTATTC AGTCCCCATT TACAGAAGTC CTTTGTAAGT
    AGCAACTGGG CTGAAACACC ACGGATAGAA AAAAGGAACG AGAGCTCTTA CTTCCTGGGA
    AATGTTCTCA CAAGCACGGC TGTGAGAGAT CAGAATAAAC GTGCTGCTCC AATAAATGAC
    TTAGAAATTC AGGCTGCCTG TGATATTGAT AATTTTGACA TAGATGGCTT TGATGATGAT
    GATGACTGGG AAGATATAAT GCATGATTTA GCGGCCAGCA GATCTTCCAC AGCTGCCTGT
    CTACCTATTA AGGAAGGTCG GCCAGTTAAG CCAGTATCAG AAAGAATTTC TTCAGCCAAG
    ACAAACTGTC TTCCAGTGGC ATCTACTGCT CAAAATAAAA ACTCAGAGTC AATTCAGAAT
    TACACTGACA AGTCGGCGCA AAATTTAGCA TCCAGAAATC TGAAACATGA ACGTTTCCAA
    AGTCTTAGTT TTCCTCATAC AAAAGAAATG ATGAAGATTT TTCATAAAAA ATTTGGCCTG
    CATAATTTTA GAACTAATCA GCTAGAGGCG ATCAATGCTG CACTGCTGGG TGAAGACTGT
    TTTATCTTAA TGCCCACTGG AGGTGGTAAG AGTCTGTGTT ACCAGCTCCC TGCCTGTGTC
    TCTCCTGGTG TCACTATTGT CATTTCTCCT TTGAGATCAC TTATAGTAGA TCAAGTCCAA
    AAGCTGACTT CCTTGGATAT TCCAGCTACA TATCTGACAG GTGATAAGAC TGACTCGGAA
    GCAACAAGTA TTTACCTGCA ATTATCCAAG AAAGACCCAA TTATAAAACT TCTATATGTT
    ACTCCAGAGA AGGTCTGTGC AAGTAATAGA CTGCTTTCCA CTCTGGAGAA TCTGTATGAG
    AGGAAGCTCC TGGCACGTTT TGTCATTGAT GAAGCGCATT GTGTCAGTCA GTGGGGACAT
    GATTTTCGCC AAGATTATAA AAGAATGAAT ATGCTTCGCC AGAAGTTTCC CTCTGTTCCA
    GTGATGGCCC TTACTGCCAC AGCTAATCCC AGGGTACAGA AGGACATCCT CACTCAGCTG
    AAGATCCTCC AGCCTCAGGT GTTTAGCATG AGCTTTAACA GACATAATCT GAAATACTAC
    GTGTTACCAA AAAAACCCAA GAAGGTGGCA TTTGATTGCT TAGAATGGAT CAGAAAGTAT
    CACCCACATG ACTCCGGGAT AATCTATTGC CTCTCCAGAC GAGAATGTGA TACAATGGCT
    GACACTTTAC AGAAAGATGG TCTTGCTGCT CTTGCTTATC ATGCTGGCCT CAGTGATTCT
    GCAAGGGATG AAGTACAGCA CAAGTGGATT AATCAGGATG GCTGCCAGGT CATCTGTGCA
    ACAATAGCGT TCGGAATGGG GATTGACAAA CCTGATGTGC GATTCGTGAT TCATGCATCT
    CTCCCTAAGT CTGTGGAGGG CTACTACCAA GAATCTGGCA GAGCTGGAAG AGATGGGGAA
    ATTTCTCACT GCCTGCTGTT CTATACCTAT CATGATGTGA CACGACTCAA GAGACTCATC
    CTGATGGAAA AAGATGGAAA CCGTCATACA AGAGAGACTC ACTTCAATAA TTTGTATAGT
    ATGGTGCATT ACTGTGAAAA TATAACAGAA TGCAGGAGAA TACAGCTTTT GGCTTACTTT
    GGCGAAAGTG GATTTAATCC TGATTTTTGT AAGAAATACC CTGATGTTTC TTGTGATAAT
    TGCTGTAAAA CAAAGGATTA TAAGACAAGA GATGTGACTG ATGATGTAAA AAATATTGTA
    AGATTTGTTC AAGAACATAG TTCATCACAA GGAACAAGAA TTAAAAACCA ATTAGGTCCT
    TCTGGGAGAT TTACTATGAA TATGCTAGTT GACATTTTCT TGGGGAGTAA GAGTGCAAAA
    ATTCAGTCGG GGATATTTGG ACAGGGATCT ACTTATTCAC GACACAATGC CGAAAGACTT
    TTTAAGAAAT TGATACTTGA CAAGATTTTG GATGAAGACT TATATATCAA TGCCAATGAC
    CAACCAATTG CGTATGTGAT GCCAGGAAAT AAAGCCCAAA CTGTACTAAA TGGGCATTTA
    AAGGTAGACT TTATAGAAAC GGAAAATTCT ACCAGTATAA AAAAACAGAA AGCTGCTTTA
    GTGGCAAAGA TGTCTCAGAG AGAAGAAGTG GTAAAGAAGT GTCTTGGAGA ACTGACAGAA
    GTGTGTAAAT CTCTGGGAAA AGTTTTTGGT GTCCACTACT TTAATATTTT TAATACTGTG
    ACTCTCAAGA AGCTTGCAGA ATCTCTATCT TCTGATCCCG AGGTTTTGCT TCAAATTGAT
    GGTGTTACTG AAGACAAACT GGAAAAATAT GGTGCAGAAG TGATTCCAAT ATTACAGAAA
    TACTCTGAGT GGACATTGCC AGCTGACGAT GGTTCCCCAC GAATCAGCCC GAGCAGCAGC
    AGAAGCACCA GGAGAAGTGC CCCTGAGGAG TTCGACGAGG AAACATCCGT ATCTTCTCAC
    TACTTTGCAA ATAAAACCAA AAATGAAAGA AAGAGGAAAA GGATGTCAGC CACCCAGAGG
    TCTAAGAGGA GGAGAACTGG CTTTGGTGGC TCCAAGACAA AAGGGGGGTC CACCGCATGC
    AGAAAGACAT CTTCTAAAAG TAAATCCTCC AACATATTTG GACCTCATTC ATCTTTGCAT
    GGTTCTCAAG CAGCATCAGG AGCCGCTAGA AAATTGGGGA TTATGGCTCC CCCAAAGCCT
    ATAAATAGAC CGTTTCTTAA GCCTTCATAT GCATTTTCAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967917.1
    CDS114..3275
    Translation

    Target ORF information:

    RefSeq Version XM_014112442.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Bloom syndrome, RecQ helicase-like (BLM), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112442.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACCAGA TCTGTAAATT AGTAGATACT ATTCCTGATG ATGAACTGAA AGCTCTGACT 
    TGTGGGAATG CACTGCATCA GCAGCGGGAC ATAAGAAGGA AGCTCCTAGC TGAAGCCGAC
    TTTAATACAA GTGATGCCAG TGTTCTTGCT TCGGTGTGGA CACGCGGGCC TGATTCACTG
    GGAAGCCCTG TGGAGGATAT GGCATCTCTT TCTGCTCCTG ATGTGTTTCC AAAGGGCAAT
    TCCCATCCTA CCGGGAATTC TGTTAAGGAG TTAAATTTTC CACACCTTCC CTCACATTCC
    AGTTCCACTG GGGAGTGTTT ACTGGCTACT ACCCCAGGAA AGACAGGATT CTCCGGCACC
    ATGAAGAATC CCTCTGAAAG ACTGTTATTC AGTCCCCATT TACAGAAGTC CTTTGTAAGT
    AGCAACTGGG CTGAAACACC ACGGATAGAA AAAAGGAACG AGAGCTCTTA CTTCCTGGGA
    AATGTTCTCA CAAGCACGGC TGTGAGAGAT CAGAATAAAC GTGCTGCTCC AATAAATGAC
    TTAGAAATTC AGGCTGCCTG TGATATTGAT AATTTTGACA TAGATGGCTT TGATGATGAT
    GATGACTGGG AAGATATAAT GCATGATTTA GCGGCCAGCA GATCTTCCAC AGCTGCCTGT
    CTACCTATTA AGGAAGGTCG GCCAGTTAAG CCAGTATCAG AAAGAATTTC TTCAGCCAAG
    ACAAACTGTC TTCCAGTGGC ATCTACTGCT CAAAATAAAA ACTCAGAGTC AATTCAGAAT
    TACACTGACA AGTCGGCGCA AAATTTAGCA TCCAGAAATC TGAAACATGA ACGTTTCCAA
    AGTCTTAGTT TTCCTCATAC AAAAGAAATG ATGAAGATTT TTCATAAAAA ATTTGGCCTG
    CATAATTTTA GAACTAATCA GCTAGAGGCG ATCAATGCTG CACTGCTGGG TGAAGACTGT
    TTTATCTTAA TGCCCACTGG AGGTGGTAAG AGTCTGTGTT ACCAGCTCCC TGCCTGTGTC
    TCTCCTGGTG TCACTATTGT CATTTCTCCT TTGAGATCAC TTATAGTAGA TCAAGTCCAA
    AAGCTGACTT CCTTGGATAT TCCAGCTACA TATCTGACAG GTGATAAGAC TGACTCGGAA
    GCAACAAGTA TTTACCTGCA ATTATCCAAG AAAGACCCAA TTATAAAACT TCTATATGTT
    ACTCCAGAGA AGGTCTGTGC AAGTAATAGA CTGCTTTCCA CTCTGGAGAA TCTGTATGAG
    AGGAAGCTCC TGGCACGTTT TGTCATTGAT GAAGCGCATT GTGTCAGTCA GTGGGGACAT
    GATTTTCGCC AAGATTATAA AAGAATGAAT ATGCTTCGCC AGAAGTTTCC CTCTGTTCCA
    GTGATGGCCC TTACTGCCAC AGCTAATCCC AGGGTACAGA AGGACATCCT CACTCAGCTG
    AAGATCCTCC AGCCTCAGGT GTTTAGCATG AGCTTTAACA GACATAATCT GAAATACTAC
    GTGTTACCAA AAAAACCCAA GAAGGTGGCA TTTGATTGCT TAGAATGGAT CAGAAAGTAT
    CACCCACATG ACTCCGGGAT AATCTATTGC CTCTCCAGAC GAGAATGTGA TACAATGGCT
    GACACTTTAC AGAAAGATGG TCTTGCTGCT CTTGCTTATC ATGCTGGCCT CAGTGATTCT
    GCAAGGGATG AAGTACAGCA CAAGTGGATT AATCAGGATG GCTGCCAGGT CATCTGTGCA
    ACAATAGCGT TCGGAATGGG GATTGACAAA CCTGATGTGC GATTCGTGAT TCATGCATCT
    CTCCCTAAGT CTGTGGAGGG CTACTACCAA GAATCTGGCA GAGCTGGAAG AGATGGGGAA
    ATTTCTCACT GCCTGCTGTT CTATACCTAT CATGATGTGA CACGACTCAA GAGACTCATC
    CTGATGGAAA AAGATGGAAA CCGTCATACA AGAGAGACTC ACTTCAATAA TTTGTATAGT
    ATGGTGCATT ACTGTGAAAA TATAACAGAA TGCAGGAGAA TACAGCTTTT GGCTTACTTT
    GGCGAAAGTG GATTTAATCC TGATTTTTGT AAGAAATACC CTGATGTTTC TTGTGATAAT
    TGCTGTAAAA CAAAGGATTA TAAGACAAGA GATGTGACTG ATGATGTAAA AAATATTGTA
    AGATTTGTTC AAGAACATAG TTCATCACAA GGAACAAGAA TTAAAAACCA ATTAGGTCCT
    TCTGGGAGAT TTACTATGAA TATGCTAGTT GACATTTTCT TGGGGAGTAA GAGTGCAAAA
    ATTCAGTCGG GGATATTTGG ACAGGGATCT ACTTATTCAC GACACAATGC CGAAAGACTT
    TTTAAGAAAT TGATACTTGA CAAGATTTTG GATGAAGACT TATATATCAA TGCCAATGAC
    CAACCAATTG CGTATGTGAT GCCAGGAAAT AAAGCCCAAA CTGTACTAAA TGGGCATTTA
    AAGGTAGACT TTATAGAAAC GGAAAATTCT ACCAGTATAA AAAAACAGAA AGCTGCTTTA
    GTGGCAAAGA TGTCTCAGAG AGAAGAAGTG GTAAAGAAGT GTCTTGGAGA ACTGACAGAA
    GTGTGTAAAT CTCTGGGAAA AGTTTTTGGT GTCCACTACT TTAATATTTT TAATACTGTG
    ACTCTCAAGA AGCTTGCAGA ATCTCTATCT TCTGATCCCG AGGTTTTGCT TCAAATTGAT
    GGTGTTACTG AAGACAAACT GGAAAAATAT GGTGCAGAAG TGATTCCAAT ATTACAGAAA
    TACTCTGAGT GGACATTGCC AGCTGACGAT GGTTCCCCAC GAATCAGCCC GAGCAGCAGC
    AGAAGCACCA GGAGAAGTGC CCCTGAGGAG TTCGACGAGG AAACATCCGT ATCTTCTCAC
    TACTTTGCAA ATAAAACCAA AAATGAAAGA AAGAGGAAAA GGATGTCAGC CACCCAGAGG
    TCTAAGAGGA GGAGAACTGG CTTTGGTGGC TCCAAGACAA AAGGGGGGTC CACCGCATGC
    AGAAAGACAT CTTCTAAAAG TAAATCCTCC AACATATTTG GACCTCATTC ATCTTTGCAT
    GGTTCTCAAG CAGCATCAGG AGCCGCTAGA AAATTGGGGA TTATGGCTCC CCCAAAGCCT
    ATAAATAGAC CGTTTCTTAA GCCTTCATAT GCATTTTCAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35736
    Clone ID Related Accession (Same CDS sequence) XM_014112442.1 , XM_014112442.2
    Accession Version XM_014112442.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product Bloom syndrome protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACCAGA TCTGTAAATT AGTAGATACT ATTCCTGATG ATGAACTGAA AGCTCTGACT 
    TGTGGGAATG CACTGCATCA GCAGCGGGAC ATAAGAAGGA AGCTCCTAGC TGAAGCCGAC
    TTTAATACAA GTGATGCCAG TGTTCTTGCT TCGGTGTGGA CACGCGGGCC TGATTCACTG
    GGAAGCCCTG TGGAGGATAT GGCATCTCTT TCTGCTCCTG ATGTGTTTCC AAAGGGCAAT
    TCCCATCCTA CCGGGAATTC TGTTAAGGAG TTAAATTTTC CACACCTTCC CTCACATTCC
    AGTTCCACTG GGGAGTGTTT ACTGGCTACT ACCCCAGGAA AGACAGGATT CTCCGGCACC
    ATGAAGAATC CCTCTGAAAG ACTGTTATTC AGTCCCCATT TACAGAAGTC CTTTGTAAGT
    AGCAACTGGG CTGAAACACC ACGGATAGAA AAAAGGAACG AGAGCTCTTA CTTCCTGGGA
    AATGTTCTCA CAAGCACGGC TGTGAGAGAT CAGAATAAAC GTGCTGCTCC AATAAATGAC
    TTAGAAATTC AGGCTGCCTG TGATATTGAT AATTTTGACA TAGATGGCTT TGATGATGAT
    GATGACTGGG AAGATATAAT GCATGATTTA GCGGCCAGCA GATCTTCCAC AGCTGCCTGT
    CTACCTATTA AGGAAGGTCG GCCAGTTAAG CCAGTATCAG AAAGAATTTC TTCAGCCAAG
    ACAAACTGTC TTCCAGTGGC ATCTACTGCT CAAAATAAAA ACTCAGAGTC AATTCAGAAT
    TACACTGACA AGTCGGCGCA AAATTTAGCA TCCAGAAATC TGAAACATGA ACGTTTCCAA
    AGTCTTAGTT TTCCTCATAC AAAAGAAATG ATGAAGATTT TTCATAAAAA ATTTGGCCTG
    CATAATTTTA GAACTAATCA GCTAGAGGCG ATCAATGCTG CACTGCTGGG TGAAGACTGT
    TTTATCTTAA TGCCCACTGG AGGTGGTAAG AGTCTGTGTT ACCAGCTCCC TGCCTGTGTC
    TCTCCTGGTG TCACTATTGT CATTTCTCCT TTGAGATCAC TTATAGTAGA TCAAGTCCAA
    AAGCTGACTT CCTTGGATAT TCCAGCTACA TATCTGACAG GTGATAAGAC TGACTCGGAA
    GCAACAAGTA TTTACCTGCA ATTATCCAAG AAAGACCCAA TTATAAAACT TCTATATGTT
    ACTCCAGAGA AGGTCTGTGC AAGTAATAGA CTGCTTTCCA CTCTGGAGAA TCTGTATGAG
    AGGAAGCTCC TGGCACGTTT TGTCATTGAT GAAGCGCATT GTGTCAGTCA GTGGGGACAT
    GATTTTCGCC AAGATTATAA AAGAATGAAT ATGCTTCGCC AGAAGTTTCC CTCTGTTCCA
    GTGATGGCCC TTACTGCCAC AGCTAATCCC AGGGTACAGA AGGACATCCT CACTCAGCTG
    AAGATCCTCC AGCCTCAGGT GTTTAGCATG AGCTTTAACA GACATAATCT GAAATACTAC
    GTGTTACCAA AAAAACCCAA GAAGGTGGCA TTTGATTGCT TAGAATGGAT CAGAAAGTAT
    CACCCACATG ACTCCGGGAT AATCTATTGC CTCTCCAGAC GAGAATGTGA TACAATGGCT
    GACACTTTAC AGAAAGATGG TCTTGCTGCT CTTGCTTATC ATGCTGGCCT CAGTGATTCT
    GCAAGGGATG AAGTACAGCA CAAGTGGATT AATCAGGATG GCTGCCAGGT CATCTGTGCA
    ACAATAGCGT TCGGAATGGG GATTGACAAA CCTGATGTGC GATTCGTGAT TCATGCATCT
    CTCCCTAAGT CTGTGGAGGG CTACTACCAA GAATCTGGCA GAGCTGGAAG AGATGGGGAA
    ATTTCTCACT GCCTGCTGTT CTATACCTAT CATGATGTGA CACGACTCAA GAGACTCATC
    CTGATGGAAA AAGATGGAAA CCGTCATACA AGAGAGACTC ACTTCAATAA TTTGTATAGT
    ATGGTGCATT ACTGTGAAAA TATAACAGAA TGCAGGAGAA TACAGCTTTT GGCTTACTTT
    GGCGAAAGTG GATTTAATCC TGATTTTTGT AAGAAATACC CTGATGTTTC TTGTGATAAT
    TGCTGTAAAA CAAAGGATTA TAAGACAAGA GATGTGACTG ATGATGTAAA AAATATTGTA
    AGATTTGTTC AAGAACATAG TTCATCACAA GGAACAAGAA TTAAAAACCA ATTAGGTCCT
    TCTGGGAGAT TTACTATGAA TATGCTAGTT GACATTTTCT TGGGGAGTAA GAGTGCAAAA
    ATTCAGTCGG GGATATTTGG ACAGGGATCT ACTTATTCAC GACACAATGC CGAAAGACTT
    TTTAAGAAAT TGATACTTGA CAAGATTTTG GATGAAGACT TATATATCAA TGCCAATGAC
    CAACCAATTG CGTATGTGAT GCCAGGAAAT AAAGCCCAAA CTGTACTAAA TGGGCATTTA
    AAGGTAGACT TTATAGAAAC GGAAAATTCT ACCAGTATAA AAAAACAGAA AGCTGCTTTA
    GTGGCAAAGA TGTCTCAGAG AGAAGAAGTG GTAAAGAAGT GTCTTGGAGA ACTGACAGAA
    GTGTGTAAAT CTCTGGGAAA AGTTTTTGGT GTCCACTACT TTAATATTTT TAATACTGTG
    ACTCTCAAGA AGCTTGCAGA ATCTCTATCT TCTGATCCCG AGGTTTTGCT TCAAATTGAT
    GGTGTTACTG AAGACAAACT GGAAAAATAT GGTGCAGAAG TGATTCCAAT ATTACAGAAA
    TACTCTGAGT GGACATTGCC AGCTGACGAT GGTTCCCCAC GAATCAGCCC GAGCAGCAGC
    AGAAGCACCA GGAGAAGTGC CCCTGAGGAG TTCGACGAGG AAACATCCGT ATCTTCTCAC
    TACTTTGCAA ATAAAACCAA AAATGAAAGA AAGAGGAAAA GGATGTCAGC CACCCAGAGG
    TCTAAGAGGA GGAGAACTGG CTTTGGTGGC TCCAAGACAA AAGGGGGGTC CACCGCATGC
    AGAAAGACAT CTTCTAAAAG TAAATCCTCC AACATATTTG GACCTCATTC ATCTTTGCAT
    GGTTCTCAAG CAGCATCAGG AGCCGCTAGA AAATTGGGGA TTATGGCTCC CCCAAAGCCT
    ATAAATAGAC CGTTTCTTAA GCCTTCATAT GCATTTTCAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967917.1
    CDS155..3316
    Translation

    Target ORF information:

    RefSeq Version XM_014112442.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Bloom syndrome RecQ like helicase (BLM), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112442.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGACCAGA TCTGTAAATT AGTAGATACT ATTCCTGATG ATGAACTGAA AGCTCTGACT 
    TGTGGGAATG CACTGCATCA GCAGCGGGAC ATAAGAAGGA AGCTCCTAGC TGAAGCCGAC
    TTTAATACAA GTGATGCCAG TGTTCTTGCT TCGGTGTGGA CACGCGGGCC TGATTCACTG
    GGAAGCCCTG TGGAGGATAT GGCATCTCTT TCTGCTCCTG ATGTGTTTCC AAAGGGCAAT
    TCCCATCCTA CCGGGAATTC TGTTAAGGAG TTAAATTTTC CACACCTTCC CTCACATTCC
    AGTTCCACTG GGGAGTGTTT ACTGGCTACT ACCCCAGGAA AGACAGGATT CTCCGGCACC
    ATGAAGAATC CCTCTGAAAG ACTGTTATTC AGTCCCCATT TACAGAAGTC CTTTGTAAGT
    AGCAACTGGG CTGAAACACC ACGGATAGAA AAAAGGAACG AGAGCTCTTA CTTCCTGGGA
    AATGTTCTCA CAAGCACGGC TGTGAGAGAT CAGAATAAAC GTGCTGCTCC AATAAATGAC
    TTAGAAATTC AGGCTGCCTG TGATATTGAT AATTTTGACA TAGATGGCTT TGATGATGAT
    GATGACTGGG AAGATATAAT GCATGATTTA GCGGCCAGCA GATCTTCCAC AGCTGCCTGT
    CTACCTATTA AGGAAGGTCG GCCAGTTAAG CCAGTATCAG AAAGAATTTC TTCAGCCAAG
    ACAAACTGTC TTCCAGTGGC ATCTACTGCT CAAAATAAAA ACTCAGAGTC AATTCAGAAT
    TACACTGACA AGTCGGCGCA AAATTTAGCA TCCAGAAATC TGAAACATGA ACGTTTCCAA
    AGTCTTAGTT TTCCTCATAC AAAAGAAATG ATGAAGATTT TTCATAAAAA ATTTGGCCTG
    CATAATTTTA GAACTAATCA GCTAGAGGCG ATCAATGCTG CACTGCTGGG TGAAGACTGT
    TTTATCTTAA TGCCCACTGG AGGTGGTAAG AGTCTGTGTT ACCAGCTCCC TGCCTGTGTC
    TCTCCTGGTG TCACTATTGT CATTTCTCCT TTGAGATCAC TTATAGTAGA TCAAGTCCAA
    AAGCTGACTT CCTTGGATAT TCCAGCTACA TATCTGACAG GTGATAAGAC TGACTCGGAA
    GCAACAAGTA TTTACCTGCA ATTATCCAAG AAAGACCCAA TTATAAAACT TCTATATGTT
    ACTCCAGAGA AGGTCTGTGC AAGTAATAGA CTGCTTTCCA CTCTGGAGAA TCTGTATGAG
    AGGAAGCTCC TGGCACGTTT TGTCATTGAT GAAGCGCATT GTGTCAGTCA GTGGGGACAT
    GATTTTCGCC AAGATTATAA AAGAATGAAT ATGCTTCGCC AGAAGTTTCC CTCTGTTCCA
    GTGATGGCCC TTACTGCCAC AGCTAATCCC AGGGTACAGA AGGACATCCT CACTCAGCTG
    AAGATCCTCC AGCCTCAGGT GTTTAGCATG AGCTTTAACA GACATAATCT GAAATACTAC
    GTGTTACCAA AAAAACCCAA GAAGGTGGCA TTTGATTGCT TAGAATGGAT CAGAAAGTAT
    CACCCACATG ACTCCGGGAT AATCTATTGC CTCTCCAGAC GAGAATGTGA TACAATGGCT
    GACACTTTAC AGAAAGATGG TCTTGCTGCT CTTGCTTATC ATGCTGGCCT CAGTGATTCT
    GCAAGGGATG AAGTACAGCA CAAGTGGATT AATCAGGATG GCTGCCAGGT CATCTGTGCA
    ACAATAGCGT TCGGAATGGG GATTGACAAA CCTGATGTGC GATTCGTGAT TCATGCATCT
    CTCCCTAAGT CTGTGGAGGG CTACTACCAA GAATCTGGCA GAGCTGGAAG AGATGGGGAA
    ATTTCTCACT GCCTGCTGTT CTATACCTAT CATGATGTGA CACGACTCAA GAGACTCATC
    CTGATGGAAA AAGATGGAAA CCGTCATACA AGAGAGACTC ACTTCAATAA TTTGTATAGT
    ATGGTGCATT ACTGTGAAAA TATAACAGAA TGCAGGAGAA TACAGCTTTT GGCTTACTTT
    GGCGAAAGTG GATTTAATCC TGATTTTTGT AAGAAATACC CTGATGTTTC TTGTGATAAT
    TGCTGTAAAA CAAAGGATTA TAAGACAAGA GATGTGACTG ATGATGTAAA AAATATTGTA
    AGATTTGTTC AAGAACATAG TTCATCACAA GGAACAAGAA TTAAAAACCA ATTAGGTCCT
    TCTGGGAGAT TTACTATGAA TATGCTAGTT GACATTTTCT TGGGGAGTAA GAGTGCAAAA
    ATTCAGTCGG GGATATTTGG ACAGGGATCT ACTTATTCAC GACACAATGC CGAAAGACTT
    TTTAAGAAAT TGATACTTGA CAAGATTTTG GATGAAGACT TATATATCAA TGCCAATGAC
    CAACCAATTG CGTATGTGAT GCCAGGAAAT AAAGCCCAAA CTGTACTAAA TGGGCATTTA
    AAGGTAGACT TTATAGAAAC GGAAAATTCT ACCAGTATAA AAAAACAGAA AGCTGCTTTA
    GTGGCAAAGA TGTCTCAGAG AGAAGAAGTG GTAAAGAAGT GTCTTGGAGA ACTGACAGAA
    GTGTGTAAAT CTCTGGGAAA AGTTTTTGGT GTCCACTACT TTAATATTTT TAATACTGTG
    ACTCTCAAGA AGCTTGCAGA ATCTCTATCT TCTGATCCCG AGGTTTTGCT TCAAATTGAT
    GGTGTTACTG AAGACAAACT GGAAAAATAT GGTGCAGAAG TGATTCCAAT ATTACAGAAA
    TACTCTGAGT GGACATTGCC AGCTGACGAT GGTTCCCCAC GAATCAGCCC GAGCAGCAGC
    AGAAGCACCA GGAGAAGTGC CCCTGAGGAG TTCGACGAGG AAACATCCGT ATCTTCTCAC
    TACTTTGCAA ATAAAACCAA AAATGAAAGA AAGAGGAAAA GGATGTCAGC CACCCAGAGG
    TCTAAGAGGA GGAGAACTGG CTTTGGTGGC TCCAAGACAA AAGGGGGGTC CACCGCATGC
    AGAAAGACAT CTTCTAAAAG TAAATCCTCC AACATATTTG GACCTCATTC ATCTTTGCAT
    GGTTCTCAAG CAGCATCAGG AGCCGCTAGA AAATTGGGGA TTATGGCTCC CCCAAAGCCT
    ATAAATAGAC CGTTTCTTAA GCCTTCATAT GCATTTTCAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04302
    Clone ID Related Accession (Same CDS sequence) XM_003434379.4 , XM_003434379.3
    Accession Version XM_003434379.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4263bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product Bloom syndrome protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003434379.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGCCG TTCCCCAGAA TAATCTACAG GAGCAACTAG AACGCCATTC AGCCAGAAAA 
    CTTAGTAACA AATTAAGTCC TTCCAAACCG AAATCTTCAG TTTTCACTTT TAAGAAGAAA
    ACATCAGCCA ACAATGTGTC TGTCACTAGT GTGTCAGTAG CAAAAACACC TGTGTTAAAT
    GACAAAGATG TTAATGTCAC TGAGGCCTTT CCCTTCAGTG AACCTCTGCC CCACGCCACA
    AGTCAGCAGA CAAGGCCCAG TGACCTCAAA AATACTCCAG CAGGACAGCA AACCAGGAGA
    ACTGTTTCAA AACCATTATT GCCAGATTTG TCACTGGTTT CACGAGTTTT ATGTAATACC
    CAGAACACAC CGATTGTAAA GAAATCCAGT AATGCTACTT TCAAGAAATT GGAATTTAGC
    TCTTCATCAG ATTCTTTTGT TACCATCAAT GATTGGGATG ATATGGATGA CTTTGATACT
    TCTGGAAATT CAAAGCTTGT TACACCATGC AAAAATCACT TTGTGAGAGT TAGCACTGCT
    CAGAAGTCAA AAAAACCTAA GAGAAACTTT TTGAAAGCAC AGCTTCCTCA AGCAAATACA
    GTAAAGGCTG ATTTGACTCC ATCCTCCTTT GAAAGTAAGC AAGCATGTTT GACTAAAAAA
    CAGAACGACG ACTCGGAATG GTTTAGTAAT GATGTGATTT GCATTGATGA TGACCCCAAT
    CCTGAAGAGC TTATACATGG AGATCCTCAG GAGAGTCATT CTTTAAAAAC TCATTTGGAA
    GAGGAAAGAG ATAGCGAAAA GAAGAACTGG GAAGAAACAG AGTTATGTTC ATTGGAGAAG
    TCGCCATGTG TAGAGCTTGA CGAAGATGAT TACGATACAG ATTTTGTTCC ACCTTCTCCA
    GAAGTAATTT CTGCCTCTTC TTCCTCTTTA AAGTGTTTTA GTATGTTAAA GGACCTTGAT
    GCCTCTGGCA CAGAGGATGG TCCTAGCACA TCCGAGGACT TGCCAACACC CAAGGGGATG
    ACAACACAGC AGCCCAATCA AGAGACCAGC ACAAACTGTG ACGCTAGACA GCCAAGTTTA
    CAGCAGCAGC TTATTCACGT GATGGACCAG ATCTGTAAAT TAGTAGATAC TATTCCTGAT
    GATGAACTGA AAGCTCTGAC TTGTGGGAAT GCACTGCATC AGCAGCGGGA CATAAGAAGG
    AAGCTCCTAG CTGAAGCCGA CTTTAATACA AGTGATGCCA GTGTTCTTGC TTCGGTGTGG
    ACACGCGGGC CTGATTCACT GGGAAGCCCT GTGGAGGATA TGGCATCTCT TTCTGCTCCT
    GATGTGTTTC CAAAGGGCAA TTCCCATCCT ACCGGGAATT CTGTTAAGGA GTTAAATTTT
    CCACACCTTC CCTCACATTC CAGTTCCACT GGGGAGTGTT TACTGGCTAC TACCCCAGGA
    AAGACAGGAT TCTCCGGCAC CATGAAGAAT CCCTCTGAAA GACTGTTATT CAGTCCCCAT
    TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CACGGATAGA AAAAAGGAAC
    GAGAGCTCTT ACTTCCTGGG AAATGTTCTC ACAAGCACGG CTGTGAGAGA TCAGAATAAA
    CGTGCTGCTC CAATAAATGA CTTAGAAATT CAGGCTGCCT GTGATATTGA TAATTTTGAC
    ATAGATGGCT TTGATGATGA TGATGACTGG GAAGATATAA TGCATGATTT AGCGGCCAGC
    AGATCTTCCA CAGCTGCCTG TCTACCTATT AAGGAAGGTC GGCCAGTTAA GCCAGTATCA
    GAAAGAATTT CTTCAGCCAA GACAAACTGT CTTCCAGTGG CATCTACTGC TCAAAATAAA
    AACTCAGAGT CAATTCAGAA TTACACTGAC AAGTCGGCGC AAAATTTAGC ATCCAGAAAT
    CTGAAACATG AACGTTTCCA AAGTCTTAGT TTTCCTCATA CAAAAGAAAT GATGAAGATT
    TTTCATAAAA AATTTGGCCT GCATAATTTT AGAACTAATC AGCTAGAGGC GATCAATGCT
    GCACTGCTGG GTGAAGACTG TTTTATCTTA ATGCCCACTG GAGGTGGTAA GAGTCTGTGT
    TACCAGCTCC CTGCCTGTGT CTCTCCTGGT GTCACTATTG TCATTTCTCC TTTGAGATCA
    CTTATAGTAG ATCAAGTCCA AAAGCTGACT TCCTTGGATA TTCCAGCTAC ATATCTGACA
    GGTGATAAGA CTGACTCGGA AGCAACAAGT ATTTACCTGC AATTATCCAA GAAAGACCCA
    ATTATAAAAC TTCTATATGT TACTCCAGAG AAGGTCTGTG CAAGTAATAG ACTGCTTTCC
    ACTCTGGAGA ATCTGTATGA GAGGAAGCTC CTGGCACGTT TTGTCATTGA TGAAGCGCAT
    TGTGTCAGTC AGTGGGGACA TGATTTTCGC CAAGATTATA AAAGAATGAA TATGCTTCGC
    CAGAAGTTTC CCTCTGTTCC AGTGATGGCC CTTACTGCCA CAGCTAATCC CAGGGTACAG
    AAGGACATCC TCACTCAGCT GAAGATCCTC CAGCCTCAGG TGTTTAGCAT GAGCTTTAAC
    AGACATAATC TGAAATACTA CGTGTTACCA AAAAAACCCA AGAAGGTGGC ATTTGATTGC
    TTAGAATGGA TCAGAAAGTA TCACCCACAT GACTCCGGGA TAATCTATTG CCTCTCCAGA
    CGAGAATGTG ATACAATGGC TGACACTTTA CAGAAAGATG GTCTTGCTGC TCTTGCTTAT
    CATGCTGGCC TCAGTGATTC TGCAAGGGAT GAAGTACAGC ACAAGTGGAT TAATCAGGAT
    GGCTGCCAGG TCATCTGTGC AACAATAGCG TTCGGAATGG GGATTGACAA ACCTGATGTG
    CGATTCGTGA TTCATGCATC TCTCCCTAAG TCTGTGGAGG GCTACTACCA AGAATCTGGC
    AGAGCTGGAA GAGATGGGGA AATTTCTCAC TGCCTGCTGT TCTATACCTA TCATGATGTG
    ACACGACTCA AGAGACTCAT CCTGATGGAA AAAGATGGAA ACCGTCATAC AAGAGAGACT
    CACTTCAATA ATTTGTATAG TATGGTGCAT TACTGTGAAA ATATAACAGA ATGCAGGAGA
    ATACAGCTTT TGGCTTACTT TGGCGAAAGT GGATTTAATC CTGATTTTTG TAAGAAATAC
    CCTGATGTTT CTTGTGATAA TTGCTGTAAA ACAAAGGATT ATAAGACAAG AGATGTGACT
    GATGATGTAA AAAATATTGT AAGATTTGTT CAAGAACATA GTTCATCACA AGGAACAAGA
    ATTAAAAACC AATTAGGTCC TTCTGGGAGA TTTACTATGA ATATGCTAGT TGACATTTTC
    TTGGGGAGTA AGAGTGCAAA AATTCAGTCG GGGATATTTG GACAGGGATC TACTTATTCA
    CGACACAATG CCGAAAGACT TTTTAAGAAA TTGATACTTG ACAAGATTTT GGATGAAGAC
    TTATATATCA ATGCCAATGA CCAACCAATT GCGTATGTGA TGCCAGGAAA TAAAGCCCAA
    ACTGTACTAA ATGGGCATTT AAAGGTAGAC TTTATAGAAA CGGAAAATTC TACCAGTATA
    AAAAAACAGA AAGCTGCTTT AGTGGCAAAG ATGTCTCAGA GAGAAGAAGT GGTAAAGAAG
    TGTCTTGGAG AACTGACAGA AGTGTGTAAA TCTCTGGGAA AAGTTTTTGG TGTCCACTAC
    TTTAATATTT TTAATACTGT GACTCTCAAG AAGCTTGCAG AATCTCTATC TTCTGATCCC
    GAGGTTTTGC TTCAAATTGA TGGTGTTACT GAAGACAAAC TGGAAAAATA TGGTGCAGAA
    GTGATTCCAA TATTACAGAA ATACTCTGAG TGGACATTGC CAGCTGACGA TGGTTCCCCA
    CGAATCAGCC CGAGCAGCAG CAGAAGCACC AGGAGAAGTG CCCCTGAGGA GTTCGACGAG
    GAAACATCCG TATCTTCTCA CTACTTTGCA AATAAAACCA AAAATGAAAG AAAGAGGAAA
    AGGATGTCAG CCACCCAGAG GTCTAAGAGG AGGAGAACTG GCTTTGGTGG CTCCAAGACA
    AAAGGGGGGT CCACCGCATG CAGAAAGACA TCTTCTAAAA GTAAATCCTC CAACATATTT
    GGACCTCATT CATCTTTGCA TGGTTCTCAA GCAGCATCAG GAGCCGCTAG AAAATTGGGG
    ATTATGGCTC CCCCAAAGCC TATAAATAGA CCGTTTCTTA AGCCTTCATA TGCATTTTCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003434427.1
    CDS104..4366
    Translation

    Target ORF information:

    RefSeq Version XM_003434379.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Bloom syndrome RecQ like helicase (BLM), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003434379.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGCCG TTCCCCAGAA TAATCTACAG GAGCAACTAG AACGCCATTC AGCCAGAAAA 
    CTTAGTAACA AATTAAGTCC TTCCAAACCG AAATCTTCAG TTTTCACTTT TAAGAAGAAA
    ACATCAGCCA ACAATGTGTC TGTCACTAGT GTGTCAGTAG CAAAAACACC TGTGTTAAAT
    GACAAAGATG TTAATGTCAC TGAGGCCTTT CCCTTCAGTG AACCTCTGCC CCACGCCACA
    AGTCAGCAGA CAAGGCCCAG TGACCTCAAA AATACTCCAG CAGGACAGCA AACCAGGAGA
    ACTGTTTCAA AACCATTATT GCCAGATTTG TCACTGGTTT CACGAGTTTT ATGTAATACC
    CAGAACACAC CGATTGTAAA GAAATCCAGT AATGCTACTT TCAAGAAATT GGAATTTAGC
    TCTTCATCAG ATTCTTTTGT TACCATCAAT GATTGGGATG ATATGGATGA CTTTGATACT
    TCTGGAAATT CAAAGCTTGT TACACCATGC AAAAATCACT TTGTGAGAGT TAGCACTGCT
    CAGAAGTCAA AAAAACCTAA GAGAAACTTT TTGAAAGCAC AGCTTCCTCA AGCAAATACA
    GTAAAGGCTG ATTTGACTCC ATCCTCCTTT GAAAGTAAGC AAGCATGTTT GACTAAAAAA
    CAGAACGACG ACTCGGAATG GTTTAGTAAT GATGTGATTT GCATTGATGA TGACCCCAAT
    CCTGAAGAGC TTATACATGG AGATCCTCAG GAGAGTCATT CTTTAAAAAC TCATTTGGAA
    GAGGAAAGAG ATAGCGAAAA GAAGAACTGG GAAGAAACAG AGTTATGTTC ATTGGAGAAG
    TCGCCATGTG TAGAGCTTGA CGAAGATGAT TACGATACAG ATTTTGTTCC ACCTTCTCCA
    GAAGTAATTT CTGCCTCTTC TTCCTCTTTA AAGTGTTTTA GTATGTTAAA GGACCTTGAT
    GCCTCTGGCA CAGAGGATGG TCCTAGCACA TCCGAGGACT TGCCAACACC CAAGGGGATG
    ACAACACAGC AGCCCAATCA AGAGACCAGC ACAAACTGTG ACGCTAGACA GCCAAGTTTA
    CAGCAGCAGC TTATTCACGT GATGGACCAG ATCTGTAAAT TAGTAGATAC TATTCCTGAT
    GATGAACTGA AAGCTCTGAC TTGTGGGAAT GCACTGCATC AGCAGCGGGA CATAAGAAGG
    AAGCTCCTAG CTGAAGCCGA CTTTAATACA AGTGATGCCA GTGTTCTTGC TTCGGTGTGG
    ACACGCGGGC CTGATTCACT GGGAAGCCCT GTGGAGGATA TGGCATCTCT TTCTGCTCCT
    GATGTGTTTC CAAAGGGCAA TTCCCATCCT ACCGGGAATT CTGTTAAGGA GTTAAATTTT
    CCACACCTTC CCTCACATTC CAGTTCCACT GGGGAGTGTT TACTGGCTAC TACCCCAGGA
    AAGACAGGAT TCTCCGGCAC CATGAAGAAT CCCTCTGAAA GACTGTTATT CAGTCCCCAT
    TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CACGGATAGA AAAAAGGAAC
    GAGAGCTCTT ACTTCCTGGG AAATGTTCTC ACAAGCACGG CTGTGAGAGA TCAGAATAAA
    CGTGCTGCTC CAATAAATGA CTTAGAAATT CAGGCTGCCT GTGATATTGA TAATTTTGAC
    ATAGATGGCT TTGATGATGA TGATGACTGG GAAGATATAA TGCATGATTT AGCGGCCAGC
    AGATCTTCCA CAGCTGCCTG TCTACCTATT AAGGAAGGTC GGCCAGTTAA GCCAGTATCA
    GAAAGAATTT CTTCAGCCAA GACAAACTGT CTTCCAGTGG CATCTACTGC TCAAAATAAA
    AACTCAGAGT CAATTCAGAA TTACACTGAC AAGTCGGCGC AAAATTTAGC ATCCAGAAAT
    CTGAAACATG AACGTTTCCA AAGTCTTAGT TTTCCTCATA CAAAAGAAAT GATGAAGATT
    TTTCATAAAA AATTTGGCCT GCATAATTTT AGAACTAATC AGCTAGAGGC GATCAATGCT
    GCACTGCTGG GTGAAGACTG TTTTATCTTA ATGCCCACTG GAGGTGGTAA GAGTCTGTGT
    TACCAGCTCC CTGCCTGTGT CTCTCCTGGT GTCACTATTG TCATTTCTCC TTTGAGATCA
    CTTATAGTAG ATCAAGTCCA AAAGCTGACT TCCTTGGATA TTCCAGCTAC ATATCTGACA
    GGTGATAAGA CTGACTCGGA AGCAACAAGT ATTTACCTGC AATTATCCAA GAAAGACCCA
    ATTATAAAAC TTCTATATGT TACTCCAGAG AAGGTCTGTG CAAGTAATAG ACTGCTTTCC
    ACTCTGGAGA ATCTGTATGA GAGGAAGCTC CTGGCACGTT TTGTCATTGA TGAAGCGCAT
    TGTGTCAGTC AGTGGGGACA TGATTTTCGC CAAGATTATA AAAGAATGAA TATGCTTCGC
    CAGAAGTTTC CCTCTGTTCC AGTGATGGCC CTTACTGCCA CAGCTAATCC CAGGGTACAG
    AAGGACATCC TCACTCAGCT GAAGATCCTC CAGCCTCAGG TGTTTAGCAT GAGCTTTAAC
    AGACATAATC TGAAATACTA CGTGTTACCA AAAAAACCCA AGAAGGTGGC ATTTGATTGC
    TTAGAATGGA TCAGAAAGTA TCACCCACAT GACTCCGGGA TAATCTATTG CCTCTCCAGA
    CGAGAATGTG ATACAATGGC TGACACTTTA CAGAAAGATG GTCTTGCTGC TCTTGCTTAT
    CATGCTGGCC TCAGTGATTC TGCAAGGGAT GAAGTACAGC ACAAGTGGAT TAATCAGGAT
    GGCTGCCAGG TCATCTGTGC AACAATAGCG TTCGGAATGG GGATTGACAA ACCTGATGTG
    CGATTCGTGA TTCATGCATC TCTCCCTAAG TCTGTGGAGG GCTACTACCA AGAATCTGGC
    AGAGCTGGAA GAGATGGGGA AATTTCTCAC TGCCTGCTGT TCTATACCTA TCATGATGTG
    ACACGACTCA AGAGACTCAT CCTGATGGAA AAAGATGGAA ACCGTCATAC AAGAGAGACT
    CACTTCAATA ATTTGTATAG TATGGTGCAT TACTGTGAAA ATATAACAGA ATGCAGGAGA
    ATACAGCTTT TGGCTTACTT TGGCGAAAGT GGATTTAATC CTGATTTTTG TAAGAAATAC
    CCTGATGTTT CTTGTGATAA TTGCTGTAAA ACAAAGGATT ATAAGACAAG AGATGTGACT
    GATGATGTAA AAAATATTGT AAGATTTGTT CAAGAACATA GTTCATCACA AGGAACAAGA
    ATTAAAAACC AATTAGGTCC TTCTGGGAGA TTTACTATGA ATATGCTAGT TGACATTTTC
    TTGGGGAGTA AGAGTGCAAA AATTCAGTCG GGGATATTTG GACAGGGATC TACTTATTCA
    CGACACAATG CCGAAAGACT TTTTAAGAAA TTGATACTTG ACAAGATTTT GGATGAAGAC
    TTATATATCA ATGCCAATGA CCAACCAATT GCGTATGTGA TGCCAGGAAA TAAAGCCCAA
    ACTGTACTAA ATGGGCATTT AAAGGTAGAC TTTATAGAAA CGGAAAATTC TACCAGTATA
    AAAAAACAGA AAGCTGCTTT AGTGGCAAAG ATGTCTCAGA GAGAAGAAGT GGTAAAGAAG
    TGTCTTGGAG AACTGACAGA AGTGTGTAAA TCTCTGGGAA AAGTTTTTGG TGTCCACTAC
    TTTAATATTT TTAATACTGT GACTCTCAAG AAGCTTGCAG AATCTCTATC TTCTGATCCC
    GAGGTTTTGC TTCAAATTGA TGGTGTTACT GAAGACAAAC TGGAAAAATA TGGTGCAGAA
    GTGATTCCAA TATTACAGAA ATACTCTGAG TGGACATTGC CAGCTGACGA TGGTTCCCCA
    CGAATCAGCC CGAGCAGCAG CAGAAGCACC AGGAGAAGTG CCCCTGAGGA GTTCGACGAG
    GAAACATCCG TATCTTCTCA CTACTTTGCA AATAAAACCA AAAATGAAAG AAAGAGGAAA
    AGGATGTCAG CCACCCAGAG GTCTAAGAGG AGGAGAACTG GCTTTGGTGG CTCCAAGACA
    AAAGGGGGGT CCACCGCATG CAGAAAGACA TCTTCTAAAA GTAAATCCTC CAACATATTT
    GGACCTCATT CATCTTTGCA TGGTTCTCAA GCAGCATCAG GAGCCGCTAG AAAATTGGGG
    ATTATGGCTC CCCCAAAGCC TATAAATAGA CCGTTTCTTA AGCCTTCATA TGCATTTTCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04302
    Clone ID Related Accession (Same CDS sequence) XM_003434379.4 , XM_003434379.3
    Accession Version XM_003434379.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4263bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product Bloom syndrome protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003434379.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGCCG TTCCCCAGAA TAATCTACAG GAGCAACTAG AACGCCATTC AGCCAGAAAA 
    CTTAGTAACA AATTAAGTCC TTCCAAACCG AAATCTTCAG TTTTCACTTT TAAGAAGAAA
    ACATCAGCCA ACAATGTGTC TGTCACTAGT GTGTCAGTAG CAAAAACACC TGTGTTAAAT
    GACAAAGATG TTAATGTCAC TGAGGCCTTT CCCTTCAGTG AACCTCTGCC CCACGCCACA
    AGTCAGCAGA CAAGGCCCAG TGACCTCAAA AATACTCCAG CAGGACAGCA AACCAGGAGA
    ACTGTTTCAA AACCATTATT GCCAGATTTG TCACTGGTTT CACGAGTTTT ATGTAATACC
    CAGAACACAC CGATTGTAAA GAAATCCAGT AATGCTACTT TCAAGAAATT GGAATTTAGC
    TCTTCATCAG ATTCTTTTGT TACCATCAAT GATTGGGATG ATATGGATGA CTTTGATACT
    TCTGGAAATT CAAAGCTTGT TACACCATGC AAAAATCACT TTGTGAGAGT TAGCACTGCT
    CAGAAGTCAA AAAAACCTAA GAGAAACTTT TTGAAAGCAC AGCTTCCTCA AGCAAATACA
    GTAAAGGCTG ATTTGACTCC ATCCTCCTTT GAAAGTAAGC AAGCATGTTT GACTAAAAAA
    CAGAACGACG ACTCGGAATG GTTTAGTAAT GATGTGATTT GCATTGATGA TGACCCCAAT
    CCTGAAGAGC TTATACATGG AGATCCTCAG GAGAGTCATT CTTTAAAAAC TCATTTGGAA
    GAGGAAAGAG ATAGCGAAAA GAAGAACTGG GAAGAAACAG AGTTATGTTC ATTGGAGAAG
    TCGCCATGTG TAGAGCTTGA CGAAGATGAT TACGATACAG ATTTTGTTCC ACCTTCTCCA
    GAAGTAATTT CTGCCTCTTC TTCCTCTTTA AAGTGTTTTA GTATGTTAAA GGACCTTGAT
    GCCTCTGGCA CAGAGGATGG TCCTAGCACA TCCGAGGACT TGCCAACACC CAAGGGGATG
    ACAACACAGC AGCCCAATCA AGAGACCAGC ACAAACTGTG ACGCTAGACA GCCAAGTTTA
    CAGCAGCAGC TTATTCACGT GATGGACCAG ATCTGTAAAT TAGTAGATAC TATTCCTGAT
    GATGAACTGA AAGCTCTGAC TTGTGGGAAT GCACTGCATC AGCAGCGGGA CATAAGAAGG
    AAGCTCCTAG CTGAAGCCGA CTTTAATACA AGTGATGCCA GTGTTCTTGC TTCGGTGTGG
    ACACGCGGGC CTGATTCACT GGGAAGCCCT GTGGAGGATA TGGCATCTCT TTCTGCTCCT
    GATGTGTTTC CAAAGGGCAA TTCCCATCCT ACCGGGAATT CTGTTAAGGA GTTAAATTTT
    CCACACCTTC CCTCACATTC CAGTTCCACT GGGGAGTGTT TACTGGCTAC TACCCCAGGA
    AAGACAGGAT TCTCCGGCAC CATGAAGAAT CCCTCTGAAA GACTGTTATT CAGTCCCCAT
    TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CACGGATAGA AAAAAGGAAC
    GAGAGCTCTT ACTTCCTGGG AAATGTTCTC ACAAGCACGG CTGTGAGAGA TCAGAATAAA
    CGTGCTGCTC CAATAAATGA CTTAGAAATT CAGGCTGCCT GTGATATTGA TAATTTTGAC
    ATAGATGGCT TTGATGATGA TGATGACTGG GAAGATATAA TGCATGATTT AGCGGCCAGC
    AGATCTTCCA CAGCTGCCTG TCTACCTATT AAGGAAGGTC GGCCAGTTAA GCCAGTATCA
    GAAAGAATTT CTTCAGCCAA GACAAACTGT CTTCCAGTGG CATCTACTGC TCAAAATAAA
    AACTCAGAGT CAATTCAGAA TTACACTGAC AAGTCGGCGC AAAATTTAGC ATCCAGAAAT
    CTGAAACATG AACGTTTCCA AAGTCTTAGT TTTCCTCATA CAAAAGAAAT GATGAAGATT
    TTTCATAAAA AATTTGGCCT GCATAATTTT AGAACTAATC AGCTAGAGGC GATCAATGCT
    GCACTGCTGG GTGAAGACTG TTTTATCTTA ATGCCCACTG GAGGTGGTAA GAGTCTGTGT
    TACCAGCTCC CTGCCTGTGT CTCTCCTGGT GTCACTATTG TCATTTCTCC TTTGAGATCA
    CTTATAGTAG ATCAAGTCCA AAAGCTGACT TCCTTGGATA TTCCAGCTAC ATATCTGACA
    GGTGATAAGA CTGACTCGGA AGCAACAAGT ATTTACCTGC AATTATCCAA GAAAGACCCA
    ATTATAAAAC TTCTATATGT TACTCCAGAG AAGGTCTGTG CAAGTAATAG ACTGCTTTCC
    ACTCTGGAGA ATCTGTATGA GAGGAAGCTC CTGGCACGTT TTGTCATTGA TGAAGCGCAT
    TGTGTCAGTC AGTGGGGACA TGATTTTCGC CAAGATTATA AAAGAATGAA TATGCTTCGC
    CAGAAGTTTC CCTCTGTTCC AGTGATGGCC CTTACTGCCA CAGCTAATCC CAGGGTACAG
    AAGGACATCC TCACTCAGCT GAAGATCCTC CAGCCTCAGG TGTTTAGCAT GAGCTTTAAC
    AGACATAATC TGAAATACTA CGTGTTACCA AAAAAACCCA AGAAGGTGGC ATTTGATTGC
    TTAGAATGGA TCAGAAAGTA TCACCCACAT GACTCCGGGA TAATCTATTG CCTCTCCAGA
    CGAGAATGTG ATACAATGGC TGACACTTTA CAGAAAGATG GTCTTGCTGC TCTTGCTTAT
    CATGCTGGCC TCAGTGATTC TGCAAGGGAT GAAGTACAGC ACAAGTGGAT TAATCAGGAT
    GGCTGCCAGG TCATCTGTGC AACAATAGCG TTCGGAATGG GGATTGACAA ACCTGATGTG
    CGATTCGTGA TTCATGCATC TCTCCCTAAG TCTGTGGAGG GCTACTACCA AGAATCTGGC
    AGAGCTGGAA GAGATGGGGA AATTTCTCAC TGCCTGCTGT TCTATACCTA TCATGATGTG
    ACACGACTCA AGAGACTCAT CCTGATGGAA AAAGATGGAA ACCGTCATAC AAGAGAGACT
    CACTTCAATA ATTTGTATAG TATGGTGCAT TACTGTGAAA ATATAACAGA ATGCAGGAGA
    ATACAGCTTT TGGCTTACTT TGGCGAAAGT GGATTTAATC CTGATTTTTG TAAGAAATAC
    CCTGATGTTT CTTGTGATAA TTGCTGTAAA ACAAAGGATT ATAAGACAAG AGATGTGACT
    GATGATGTAA AAAATATTGT AAGATTTGTT CAAGAACATA GTTCATCACA AGGAACAAGA
    ATTAAAAACC AATTAGGTCC TTCTGGGAGA TTTACTATGA ATATGCTAGT TGACATTTTC
    TTGGGGAGTA AGAGTGCAAA AATTCAGTCG GGGATATTTG GACAGGGATC TACTTATTCA
    CGACACAATG CCGAAAGACT TTTTAAGAAA TTGATACTTG ACAAGATTTT GGATGAAGAC
    TTATATATCA ATGCCAATGA CCAACCAATT GCGTATGTGA TGCCAGGAAA TAAAGCCCAA
    ACTGTACTAA ATGGGCATTT AAAGGTAGAC TTTATAGAAA CGGAAAATTC TACCAGTATA
    AAAAAACAGA AAGCTGCTTT AGTGGCAAAG ATGTCTCAGA GAGAAGAAGT GGTAAAGAAG
    TGTCTTGGAG AACTGACAGA AGTGTGTAAA TCTCTGGGAA AAGTTTTTGG TGTCCACTAC
    TTTAATATTT TTAATACTGT GACTCTCAAG AAGCTTGCAG AATCTCTATC TTCTGATCCC
    GAGGTTTTGC TTCAAATTGA TGGTGTTACT GAAGACAAAC TGGAAAAATA TGGTGCAGAA
    GTGATTCCAA TATTACAGAA ATACTCTGAG TGGACATTGC CAGCTGACGA TGGTTCCCCA
    CGAATCAGCC CGAGCAGCAG CAGAAGCACC AGGAGAAGTG CCCCTGAGGA GTTCGACGAG
    GAAACATCCG TATCTTCTCA CTACTTTGCA AATAAAACCA AAAATGAAAG AAAGAGGAAA
    AGGATGTCAG CCACCCAGAG GTCTAAGAGG AGGAGAACTG GCTTTGGTGG CTCCAAGACA
    AAAGGGGGGT CCACCGCATG CAGAAAGACA TCTTCTAAAA GTAAATCCTC CAACATATTT
    GGACCTCATT CATCTTTGCA TGGTTCTCAA GCAGCATCAG GAGCCGCTAG AAAATTGGGG
    ATTATGGCTC CCCCAAAGCC TATAAATAGA CCGTTTCTTA AGCCTTCATA TGCATTTTCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003434427.1
    CDS77..4339
    Translation

    Target ORF information:

    RefSeq Version XM_003434379.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Bloom syndrome, RecQ helicase-like (BLM), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003434379.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTGCCG TTCCCCAGAA TAATCTACAG GAGCAACTAG AACGCCATTC AGCCAGAAAA 
    CTTAGTAACA AATTAAGTCC TTCCAAACCG AAATCTTCAG TTTTCACTTT TAAGAAGAAA
    ACATCAGCCA ACAATGTGTC TGTCACTAGT GTGTCAGTAG CAAAAACACC TGTGTTAAAT
    GACAAAGATG TTAATGTCAC TGAGGCCTTT CCCTTCAGTG AACCTCTGCC CCACGCCACA
    AGTCAGCAGA CAAGGCCCAG TGACCTCAAA AATACTCCAG CAGGACAGCA AACCAGGAGA
    ACTGTTTCAA AACCATTATT GCCAGATTTG TCACTGGTTT CACGAGTTTT ATGTAATACC
    CAGAACACAC CGATTGTAAA GAAATCCAGT AATGCTACTT TCAAGAAATT GGAATTTAGC
    TCTTCATCAG ATTCTTTTGT TACCATCAAT GATTGGGATG ATATGGATGA CTTTGATACT
    TCTGGAAATT CAAAGCTTGT TACACCATGC AAAAATCACT TTGTGAGAGT TAGCACTGCT
    CAGAAGTCAA AAAAACCTAA GAGAAACTTT TTGAAAGCAC AGCTTCCTCA AGCAAATACA
    GTAAAGGCTG ATTTGACTCC ATCCTCCTTT GAAAGTAAGC AAGCATGTTT GACTAAAAAA
    CAGAACGACG ACTCGGAATG GTTTAGTAAT GATGTGATTT GCATTGATGA TGACCCCAAT
    CCTGAAGAGC TTATACATGG AGATCCTCAG GAGAGTCATT CTTTAAAAAC TCATTTGGAA
    GAGGAAAGAG ATAGCGAAAA GAAGAACTGG GAAGAAACAG AGTTATGTTC ATTGGAGAAG
    TCGCCATGTG TAGAGCTTGA CGAAGATGAT TACGATACAG ATTTTGTTCC ACCTTCTCCA
    GAAGTAATTT CTGCCTCTTC TTCCTCTTTA AAGTGTTTTA GTATGTTAAA GGACCTTGAT
    GCCTCTGGCA CAGAGGATGG TCCTAGCACA TCCGAGGACT TGCCAACACC CAAGGGGATG
    ACAACACAGC AGCCCAATCA AGAGACCAGC ACAAACTGTG ACGCTAGACA GCCAAGTTTA
    CAGCAGCAGC TTATTCACGT GATGGACCAG ATCTGTAAAT TAGTAGATAC TATTCCTGAT
    GATGAACTGA AAGCTCTGAC TTGTGGGAAT GCACTGCATC AGCAGCGGGA CATAAGAAGG
    AAGCTCCTAG CTGAAGCCGA CTTTAATACA AGTGATGCCA GTGTTCTTGC TTCGGTGTGG
    ACACGCGGGC CTGATTCACT GGGAAGCCCT GTGGAGGATA TGGCATCTCT TTCTGCTCCT
    GATGTGTTTC CAAAGGGCAA TTCCCATCCT ACCGGGAATT CTGTTAAGGA GTTAAATTTT
    CCACACCTTC CCTCACATTC CAGTTCCACT GGGGAGTGTT TACTGGCTAC TACCCCAGGA
    AAGACAGGAT TCTCCGGCAC CATGAAGAAT CCCTCTGAAA GACTGTTATT CAGTCCCCAT
    TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CACGGATAGA AAAAAGGAAC
    GAGAGCTCTT ACTTCCTGGG AAATGTTCTC ACAAGCACGG CTGTGAGAGA TCAGAATAAA
    CGTGCTGCTC CAATAAATGA CTTAGAAATT CAGGCTGCCT GTGATATTGA TAATTTTGAC
    ATAGATGGCT TTGATGATGA TGATGACTGG GAAGATATAA TGCATGATTT AGCGGCCAGC
    AGATCTTCCA CAGCTGCCTG TCTACCTATT AAGGAAGGTC GGCCAGTTAA GCCAGTATCA
    GAAAGAATTT CTTCAGCCAA GACAAACTGT CTTCCAGTGG CATCTACTGC TCAAAATAAA
    AACTCAGAGT CAATTCAGAA TTACACTGAC AAGTCGGCGC AAAATTTAGC ATCCAGAAAT
    CTGAAACATG AACGTTTCCA AAGTCTTAGT TTTCCTCATA CAAAAGAAAT GATGAAGATT
    TTTCATAAAA AATTTGGCCT GCATAATTTT AGAACTAATC AGCTAGAGGC GATCAATGCT
    GCACTGCTGG GTGAAGACTG TTTTATCTTA ATGCCCACTG GAGGTGGTAA GAGTCTGTGT
    TACCAGCTCC CTGCCTGTGT CTCTCCTGGT GTCACTATTG TCATTTCTCC TTTGAGATCA
    CTTATAGTAG ATCAAGTCCA AAAGCTGACT TCCTTGGATA TTCCAGCTAC ATATCTGACA
    GGTGATAAGA CTGACTCGGA AGCAACAAGT ATTTACCTGC AATTATCCAA GAAAGACCCA
    ATTATAAAAC TTCTATATGT TACTCCAGAG AAGGTCTGTG CAAGTAATAG ACTGCTTTCC
    ACTCTGGAGA ATCTGTATGA GAGGAAGCTC CTGGCACGTT TTGTCATTGA TGAAGCGCAT
    TGTGTCAGTC AGTGGGGACA TGATTTTCGC CAAGATTATA AAAGAATGAA TATGCTTCGC
    CAGAAGTTTC CCTCTGTTCC AGTGATGGCC CTTACTGCCA CAGCTAATCC CAGGGTACAG
    AAGGACATCC TCACTCAGCT GAAGATCCTC CAGCCTCAGG TGTTTAGCAT GAGCTTTAAC
    AGACATAATC TGAAATACTA CGTGTTACCA AAAAAACCCA AGAAGGTGGC ATTTGATTGC
    TTAGAATGGA TCAGAAAGTA TCACCCACAT GACTCCGGGA TAATCTATTG CCTCTCCAGA
    CGAGAATGTG ATACAATGGC TGACACTTTA CAGAAAGATG GTCTTGCTGC TCTTGCTTAT
    CATGCTGGCC TCAGTGATTC TGCAAGGGAT GAAGTACAGC ACAAGTGGAT TAATCAGGAT
    GGCTGCCAGG TCATCTGTGC AACAATAGCG TTCGGAATGG GGATTGACAA ACCTGATGTG
    CGATTCGTGA TTCATGCATC TCTCCCTAAG TCTGTGGAGG GCTACTACCA AGAATCTGGC
    AGAGCTGGAA GAGATGGGGA AATTTCTCAC TGCCTGCTGT TCTATACCTA TCATGATGTG
    ACACGACTCA AGAGACTCAT CCTGATGGAA AAAGATGGAA ACCGTCATAC AAGAGAGACT
    CACTTCAATA ATTTGTATAG TATGGTGCAT TACTGTGAAA ATATAACAGA ATGCAGGAGA
    ATACAGCTTT TGGCTTACTT TGGCGAAAGT GGATTTAATC CTGATTTTTG TAAGAAATAC
    CCTGATGTTT CTTGTGATAA TTGCTGTAAA ACAAAGGATT ATAAGACAAG AGATGTGACT
    GATGATGTAA AAAATATTGT AAGATTTGTT CAAGAACATA GTTCATCACA AGGAACAAGA
    ATTAAAAACC AATTAGGTCC TTCTGGGAGA TTTACTATGA ATATGCTAGT TGACATTTTC
    TTGGGGAGTA AGAGTGCAAA AATTCAGTCG GGGATATTTG GACAGGGATC TACTTATTCA
    CGACACAATG CCGAAAGACT TTTTAAGAAA TTGATACTTG ACAAGATTTT GGATGAAGAC
    TTATATATCA ATGCCAATGA CCAACCAATT GCGTATGTGA TGCCAGGAAA TAAAGCCCAA
    ACTGTACTAA ATGGGCATTT AAAGGTAGAC TTTATAGAAA CGGAAAATTC TACCAGTATA
    AAAAAACAGA AAGCTGCTTT AGTGGCAAAG ATGTCTCAGA GAGAAGAAGT GGTAAAGAAG
    TGTCTTGGAG AACTGACAGA AGTGTGTAAA TCTCTGGGAA AAGTTTTTGG TGTCCACTAC
    TTTAATATTT TTAATACTGT GACTCTCAAG AAGCTTGCAG AATCTCTATC TTCTGATCCC
    GAGGTTTTGC TTCAAATTGA TGGTGTTACT GAAGACAAAC TGGAAAAATA TGGTGCAGAA
    GTGATTCCAA TATTACAGAA ATACTCTGAG TGGACATTGC CAGCTGACGA TGGTTCCCCA
    CGAATCAGCC CGAGCAGCAG CAGAAGCACC AGGAGAAGTG CCCCTGAGGA GTTCGACGAG
    GAAACATCCG TATCTTCTCA CTACTTTGCA AATAAAACCA AAAATGAAAG AAAGAGGAAA
    AGGATGTCAG CCACCCAGAG GTCTAAGAGG AGGAGAACTG GCTTTGGTGG CTCCAAGACA
    AAAGGGGGGT CCACCGCATG CAGAAAGACA TCTTCTAAAA GTAAATCCTC CAACATATTT
    GGACCTCATT CATCTTTGCA TGGTTCTCAA GCAGCATCAG GAGCCGCTAG AAAATTGGGG
    ATTATGGCTC CCCCAAAGCC TATAAATAGA CCGTTTCTTA AGCCTTCATA TGCATTTTCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.