HLCS cDNA ORF clone, Camelus bactrianus(Bactrian camel)

Gene
Clones
gRNAs

Summary

Gene Symbol	HLCS
Entrez Gene ID	105070401
Full Name	holocarboxylase synthetase
Gene Type	protein-coding
Organism	Camelus bactrianus(Bactrian camel)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010956833.1	XP_010955135.1	biotin--protein ligase

Pathways from BioSystems
Gene Ontology

The following HLCS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLCS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa490737	XM_010956833.1 Latest version!	Camelus bactrianus holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HLCS ( XM_010956833.1 ) cDNA ORF clone, Camelus bactrianus(Bactrian camel) -> XP_010955135.1 Camelus bactrianus holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), mRNA.

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CloneID	OCa490737
Clone ID Related Accession (Same CDS sequence)	XM_010956833.1
Accession Version	XM_010956833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-05
Organism	Camelus bactrianus(Bactrian camel)
Product	biotin--protein ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011514130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Camelus bactrianus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 19% of CDS bases ##RefSeq-Attributes-END##

ATGCGGGAGG GCGCGCGTGC CCTGGGACCA CTTGGGCGGC TGCGGTCGTC ACCAGCGCTG 
GGCCTGGATG CCACAAGTGC TATATTATCA GCAGAGCCCA GCGACAGAAC CCTGAGGGCC 
GCAGGCCTGG ACTCGGGCCC CTCGCTTAAA CAGGATCTGT TCCTCGCCGG AAGCGCCCCC 
GGGGCCCCGC GCCGTCCCTG TTCCCGGGCT GTGCACCTCG GCGGCTCTCT CCGCGAGGCC 
CTGAGGCCGC GGGCTTGGGC TCCCGGGAGG GCCAGATTCC GGGGCTCCAC TGCGTGCGGT 
GCCCGCGGGA GCCTGCAGAA GAGGACCGAA GGCGGAAACT GTGACATGCA ATCTGGTCGC 
CTCTCCACGA GGTGCCGGCG CAAGTGTATG GACGCCTGGC TCCTGGCATG TACCCTTGTG 
TCTGTGCGAT GTGGATTAGC ACCTGGCCGC GGGCTTGGGC TCCCGGGAGG GCCAGATTCC 
GGGGCTCCAC TGCGTGCGGT GCCCGCGGGA GCCTGCAGAA GAGGACCGAA GGCGGAAACT 
GTGACATGCA ATCTGGTCGC CTCTCCACGA GGTGCCGGCG CAAGTGCTGG AGTTTGGTTG 
AACCTGATTT TAGAGAAAGA CAGCAAATCC TCAGCTTTCT GTCAGTGCAT TGATGACTTG 
AACAAATGGG CCCTGTTTCT GGTTTCTCCT TTTATACTTG AAGCAGAGCA CATAGCATTT 
GTGACAGAGA GCATTTGGGT GCAAGGCGAG AATTTACAGA GACCATCATC CTCTTCAGAC 
ACCATTGTAA AGTGGTCGGA CTGTTGTTTG CCATTAGCTT GCAGACCTGG GGATCCTTAT 
CAGTTAATCG CCAGAGCAAG TGTGGATGAC TTCAGCAAGC TGGGGGTGGC GTTCCTGGAA 
GATAGACTCC AGATGGCTAA TGGGCTGATA CCCCAAAAGA TCGTCTCGGT GCACTTGCAG 
GAATCCGCTC TGAAGGAACT TAAGGATCAG GCCTCAGACA CTCAAGCCCG GACCCTACAG 
CCCAACGGGG AGTCTCCGGT TGAGAGTAAG CCTGAGCCAG ACGCTATGGA GCACATGGGC 
AGAAGTGACT CGCAGGTACT TGGATCTGCA CCCACACAGG GGAAAGGCAG TGCCCCTGGA 
GGTGAGCCTG CTGGGGAGAG CGACAGAGAA GTGGGCTCTG TTGAGCACTG TCACCTCCAT 
CTGTCCAGCT GCCATGAGTG TTTGGAGCTG GAGAACAGCA CCATCGAATC CGTCAAGTTT 
GCCTCTGCAG AGAACATTCC AGATCTTCCC TACGATTATA GCAGTGGTTT GGAGGGGATT 
GCTGACCTCA GCCTTGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTTCTCT ATGTGGGCTC CGACTCCCAG GACGCCCAGG GCAGGCTCCA GCTGGTCCGG 
TCGGCCCTGG CTGACTGCGT GGACACCGAC TGTTACACTC TCTACCACCT GTCCCCGGAA 
AGTGCGCTCA GGGACCCGTG GTCGGACAAC TGTCTGTTGC TGGTCATTGC CACCAGGGAG 
CCAGTCCCCG AGGACCTGTC CCGGAAGTTC ATGGCCTATC TTTCTCAGGG AGGGAAGGTG 
CTGGGTCTGT CTTCATCCTT CACACTTGCC GGCTTCCGAG TGATGAGCAA GGCCCCGCTG 
CAGGACACAG TGCAGAGCCT GGTGTTCTGC AAGGCCGACC AGAGCCGGGT GAAGCTCAGC 
GTCCTGAGCA GCGGTGGTGT TTACGAGGGG GGCCCTGACC AGCTGCTCAG CCTGGGCAAG 
CTCCAGGGCC ACCTGGACAG CGAGGATGAG GACAGGCTGA TTGTGCAAGT GCCTTTTGGA 
ACACGTGGAG GAGAAGCTGT GCTTTGCCAG GTGCACTTGG AGCTACCCCC CAGCTCTCCA 
ATAGTGCAAA CTCAAGAGGA TTTTGATCTG CTCAAATCGA ACAATAGCAG AAGATACGAG 
GTCCTTAAGG AGATCCTGAC GACGCTGGGC CTAAGCTGTG ACTTAACACA AGTTCCTGCC 
TTAACACCTA TTTACTTACT GTCAGTGGCT GAGGAAATCC GGGTTCCTCT TATGCAGTGG 
CTGGGGAAAC ATGTGGATCC TGAAGGAGTA ATAAAATCTA GCAAGCTCTC CCTTAAGTTT 
GTTTCATCCT ACACATCTGA AATAGAAGTC ACCCCATCTG CCATACCTGT GGTGACGGAC 
ATGGAGGCCT TCTCATCAGA AAACTTTGAC TTCGAGATCT ACCGACAAAA TTTGCAGACA 
AAGAAACTTG GGAAAACAGT TCTGTTTGCC GAAGTGACAC CTACCACGAT GAGTCTTCTG 
GATGGGTTGA TGTTTGAGAT GCCGCAGGAA ATGGGTTTAA TAGCCATCGC AGCCCGCCAG 
ACACAGGGCA AAGGGCGGGG AAGAAATGTG TGGCTGAGCC CCAAGGGATG TGCTCTTTCT 
ACCCTGCTTG TCTCCATCCC ACTGAGATCC CATCTGGGAC AGAGGATTCC ATTTGTCCAG 
CATCTGATGT CCCTGGCTGT TGTGGAGGCG GTCAGGTCCA TTCCTGAGTA TCAGGATATC 
AACTTACGAG TGAAATGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCAACACT TATGGGAGAA ACATTTTATA TACTTATCGG CTGTGGATTT 
AACGTGACTA ATAGTAACCC TACCATATGC ATCAACGATC TCATCACAGA ATACAATAAG 
CAACACAGGG CAGGACTGAA GCCCCTCAGA GCTGATTATC TGATCGCCAG AACTGTGACC 
GTGCTGGAGA AACTGATGGA CACGTTTCAG GACGAAGGGC CCAATGGCAT TCTGCCTCTG 
TATTATAAAT ACTGGGTCCA CAGTGCTCAG CAAGTCAGTC TGGGAAGCCC AGAGGGACCA 
AAGGTGTGGA TAGTCGGCCT CGATGATTCC GGGTTCCTTC TGGTTCACCA GGAAAATGGC 
AAGGTGATGA CTGTGCATCC TGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTCATCGTC 
CCCAAACAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010955135.1
CDS	1..3075
Translation	MREGARALGPLGRLRSSPALGLDATSAILSAEPSDRTLRAAGLDSGPSLKQDLFLAGSAPGAPRRPCSRAVHLGGSLREALRPRAWAPGRARFRGSTACGARGSLQKRTEGGNCDMQSGRLSTRCRRKCMDAWLLACTLVSVRCGLAPGRGLGLPGGPDSGAPLRAVPAGACRRGPKAETVTCNLVASPRGAGASAGVWLNLILEKDSKSSAFCQCIDDLNKWALFLVSPFILEAEHIAFVTESIWVQGENLQRPSSSSDTIVKWSDCCLPLACRPGDPYQLIARASVDDFSKLGVAFLEDRLQMANGLIPQKIVSVHLQESALKELKDQASDTQARTLQPNGESPVESKPEPDAMEHMGRSDSQVLGSAPTQGKGSAPGGEPAGESDREVGSVEHCHLHLSSCHECLELENSTIESVKFASAENIPDLPYDYSSGLEGIADLSLEREGRRVNITGKAPNILLYVGSDSQDAQGRLQLVRSALADCVDTDCYTLYHLSPESALRDPWSDNCLLLVIATREPVPEDLSRKFMAYLSQGGKVLGLSSSFTLAGFRVMSKAPLQDTVQSLVFCKADQSRVKLSVLSSGGVYEGGPDQLLSLGKLQGHLDSEDEDRLIVQVPFGTRGGEAVLCQVHLELPPSSPIVQTQEDFDLLKSNNSRRYEVLKEILTTLGLSCDLTQVPALTPIYLLSVAEEIRVPLMQWLGKHVDPEGVIKSSKLSLKFVSSYTSEIEVTPSAIPVVTDMEAFSSENFDFEIYRQNLQTKKLGKTVLFAEVTPTTMSLLDGLMFEMPQEMGLIAIAARQTQGKGRGRNVWLSPKGCALSTLLVSIPLRSHLGQRIPFVQHLMSLAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKLGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHRAGLKPLRADYLIARTVTVLEKLMDTFQDEGPNGILPLYYKYWVHSAQQVSLGSPEGPKVWIVGLDDSGFLLVHQENGKVMTVHPDGNSFDMLRNLIVPKQQ

Target ORF information:

RefSeq Version	XM_010956833.1
Organism	Camelus bactrianus(Bactrian camel)
Definition	Camelus bactrianus holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010956833.1

ORF Insert Sequence:

ATGCGGGAGG GCGCGCGTGC CCTGGGACCA CTTGGGCGGC TGCGGTCGTC ACCAGCGCTG 
GGCCTGGATG CCACAAGTGC TATATTATCA GCAGAGCCCA GCGACAGAAC CCTGAGGGCC 
GCAGGCCTGG ACTCGGGCCC CTCGCTTAAA CAGGATCTGT TCCTCGCCGG AAGCGCCCCC 
GGGGCCCCGC GCCGTCCCTG TTCCCGGGCT GTGCACCTCG GCGGCTCTCT CCGCGAGGCC 
CTGAGGCCGC GGGCTTGGGC TCCCGGGAGG GCCAGATTCC GGGGCTCCAC TGCGTGCGGT 
GCCCGCGGGA GCCTGCAGAA GAGGACCGAA GGCGGAAACT GTGACATGCA ATCTGGTCGC 
CTCTCCACGA GGTGCCGGCG CAAGTGTATG GACGCCTGGC TCCTGGCATG TACCCTTGTG 
TCTGTGCGAT GTGGATTAGC ACCTGGCCGC GGGCTTGGGC TCCCGGGAGG GCCAGATTCC 
GGGGCTCCAC TGCGTGCGGT GCCCGCGGGA GCCTGCAGAA GAGGACCGAA GGCGGAAACT 
GTGACATGCA ATCTGGTCGC CTCTCCACGA GGTGCCGGCG CAAGTGCTGG AGTTTGGTTG 
AACCTGATTT TAGAGAAAGA CAGCAAATCC TCAGCTTTCT GTCAGTGCAT TGATGACTTG 
AACAAATGGG CCCTGTTTCT GGTTTCTCCT TTTATACTTG AAGCAGAGCA CATAGCATTT 
GTGACAGAGA GCATTTGGGT GCAAGGCGAG AATTTACAGA GACCATCATC CTCTTCAGAC 
ACCATTGTAA AGTGGTCGGA CTGTTGTTTG CCATTAGCTT GCAGACCTGG GGATCCTTAT 
CAGTTAATCG CCAGAGCAAG TGTGGATGAC TTCAGCAAGC TGGGGGTGGC GTTCCTGGAA 
GATAGACTCC AGATGGCTAA TGGGCTGATA CCCCAAAAGA TCGTCTCGGT GCACTTGCAG 
GAATCCGCTC TGAAGGAACT TAAGGATCAG GCCTCAGACA CTCAAGCCCG GACCCTACAG 
CCCAACGGGG AGTCTCCGGT TGAGAGTAAG CCTGAGCCAG ACGCTATGGA GCACATGGGC 
AGAAGTGACT CGCAGGTACT TGGATCTGCA CCCACACAGG GGAAAGGCAG TGCCCCTGGA 
GGTGAGCCTG CTGGGGAGAG CGACAGAGAA GTGGGCTCTG TTGAGCACTG TCACCTCCAT 
CTGTCCAGCT GCCATGAGTG TTTGGAGCTG GAGAACAGCA CCATCGAATC CGTCAAGTTT 
GCCTCTGCAG AGAACATTCC AGATCTTCCC TACGATTATA GCAGTGGTTT GGAGGGGATT 
GCTGACCTCA GCCTTGAGAG AGAAGGGAGG AGAGTCAACA TCACAGGAAA GGCACCCAAC 
ATCCTTCTCT ATGTGGGCTC CGACTCCCAG GACGCCCAGG GCAGGCTCCA GCTGGTCCGG 
TCGGCCCTGG CTGACTGCGT GGACACCGAC TGTTACACTC TCTACCACCT GTCCCCGGAA 
AGTGCGCTCA GGGACCCGTG GTCGGACAAC TGTCTGTTGC TGGTCATTGC CACCAGGGAG 
CCAGTCCCCG AGGACCTGTC CCGGAAGTTC ATGGCCTATC TTTCTCAGGG AGGGAAGGTG 
CTGGGTCTGT CTTCATCCTT CACACTTGCC GGCTTCCGAG TGATGAGCAA GGCCCCGCTG 
CAGGACACAG TGCAGAGCCT GGTGTTCTGC AAGGCCGACC AGAGCCGGGT GAAGCTCAGC 
GTCCTGAGCA GCGGTGGTGT TTACGAGGGG GGCCCTGACC AGCTGCTCAG CCTGGGCAAG 
CTCCAGGGCC ACCTGGACAG CGAGGATGAG GACAGGCTGA TTGTGCAAGT GCCTTTTGGA 
ACACGTGGAG GAGAAGCTGT GCTTTGCCAG GTGCACTTGG AGCTACCCCC CAGCTCTCCA 
ATAGTGCAAA CTCAAGAGGA TTTTGATCTG CTCAAATCGA ACAATAGCAG AAGATACGAG 
GTCCTTAAGG AGATCCTGAC GACGCTGGGC CTAAGCTGTG ACTTAACACA AGTTCCTGCC 
TTAACACCTA TTTACTTACT GTCAGTGGCT GAGGAAATCC GGGTTCCTCT TATGCAGTGG 
CTGGGGAAAC ATGTGGATCC TGAAGGAGTA ATAAAATCTA GCAAGCTCTC CCTTAAGTTT 
GTTTCATCCT ACACATCTGA AATAGAAGTC ACCCCATCTG CCATACCTGT GGTGACGGAC 
ATGGAGGCCT TCTCATCAGA AAACTTTGAC TTCGAGATCT ACCGACAAAA TTTGCAGACA 
AAGAAACTTG GGAAAACAGT TCTGTTTGCC GAAGTGACAC CTACCACGAT GAGTCTTCTG 
GATGGGTTGA TGTTTGAGAT GCCGCAGGAA ATGGGTTTAA TAGCCATCGC AGCCCGCCAG 
ACACAGGGCA AAGGGCGGGG AAGAAATGTG TGGCTGAGCC CCAAGGGATG TGCTCTTTCT 
ACCCTGCTTG TCTCCATCCC ACTGAGATCC CATCTGGGAC AGAGGATTCC ATTTGTCCAG 
CATCTGATGT CCCTGGCTGT TGTGGAGGCG GTCAGGTCCA TTCCTGAGTA TCAGGATATC 
AACTTACGAG TGAAATGGCC CAACGATATT TATTACAGTG ACCTCATGAA GCTTGGTGGA 
GTTCTGGTTA ACTCAACACT TATGGGAGAA ACATTTTATA TACTTATCGG CTGTGGATTT 
AACGTGACTA ATAGTAACCC TACCATATGC ATCAACGATC TCATCACAGA ATACAATAAG 
CAACACAGGG CAGGACTGAA GCCCCTCAGA GCTGATTATC TGATCGCCAG AACTGTGACC 
GTGCTGGAGA AACTGATGGA CACGTTTCAG GACGAAGGGC CCAATGGCAT TCTGCCTCTG 
TATTATAAAT ACTGGGTCCA CAGTGCTCAG CAAGTCAGTC TGGGAAGCCC AGAGGGACCA 
AAGGTGTGGA TAGTCGGCCT CGATGATTCC GGGTTCCTTC TGGTTCACCA GGAAAATGGC 
AAGGTGATGA CTGTGCATCC TGATGGCAAC TCCTTCGACA TGCTGAGAAA CCTCATCGTC 
CCCAAACAGC AGTAG