rapgef5 cDNA ORF clone, Callorhinchus milii(elephant shark)

Gene
Clones
gRNAs

Summary

Gene Symbol	rapgef5
Entrez Gene ID	103185006
Full Name	Rap guanine nucleotide exchange factor 5
Gene Type	protein-coding
Organism	Callorhinchus milii(elephant shark)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007903319.1	XP_007901510.1	rap guanine nucleotide exchange factor 5 isoform X1
XM_007903323.1	XP_007901514.1	rap guanine nucleotide exchange factor 5 isoform X2

Pathways from BioSystems
Gene Ontology

The following rapgef5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rapgef5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa370588	XM_007903319.1 Latest version!	Callorhinchus milii Rap guanine nucleotide exchange factor (GEF) 5 (rapgef5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa370589	XM_007903323.1 Latest version!	Callorhinchus milii Rap guanine nucleotide exchange factor (GEF) 5 (rapgef5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rapgef5 ( XM_007903319.1 ) cDNA ORF clone, Callorhinchus milii(elephant shark) -> XP_007901510.1 Callorhinchus milii Rap guanine nucleotide exchange factor (GEF) 5 (rapgef5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa370588
Clone ID Related Accession (Same CDS sequence)	XM_007903319.1
Accession Version	XM_007903319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-08
Organism	Callorhinchus milii(elephant shark)
Product	rap guanine nucleotide exchange factor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006890057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callorhinchus milii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC AGAATGATAT TCCTGGCAGT CTCTGTGTCC CAGAAGTGGG ACTTTTAGCT 
GCTTTAAAAT GGAGCAGAAA TGCAAAGGAA CAAGGACATT TAAAACAGAG GATAAAAGAC 
CTTCCTTCAC TACTAAAAAA TGGATTTAAT TTCAGGAAGA AGCAGGCAGA TATGCAGATG 
GCCAGTTCAG AAAAGAATGC AGAATCGCAC CCATCCAATT CCAACAGAAA GATTTATTCT 
GAGACCATCT ACTGTGCTGG GCGAGCCCTT AGAGACTTCT TCATAATCCT TGCTCCTCAT 
CTCATAAACG ATCGAGTAAC CAGTACAGGA AAATACAAGA GAAGCTGTGT GGGGTTTGAG 
ATGGTGGACT GGCTGCAGGA CCAGTGTCTG TTCATCCTCA ACCGGGCGAA TGCAGAGAGT 
ATCTGGCAGA TTCTCCTGGA GCTGGGAATT CTATCTTCAG CAACGGGAAA GCAACAGAAT 
TTTCAGGACG AAGATGCTTA TTACCAGTTT TCGAGGGATG AATGTGATGT CCCTGCCTGC 
CCTTTTGGAA AGGAGGAAGA GTGGAAAAAC GGTGTGAGAC TTCTTGCTCA CATGGTCCCT 
TATGTCCAGT CCAGGACAAC AATGTTAACA TCAGAACGAG AATCAGAAGA TGAGAAAGCA 
GAACCGTGTG ATGAAGTTCT CCAGCTTCAA GCCTTGGCTC TCTTATCAGC AACGGTGAAG 
AAGGAGCTGG CTGCTGTTAT TGCTTCAAAA GGAGGAACAT CATTGTGCGA CCAGGAGGAT 
GAAAGAGTTC TCAACCAACA AGATATGAAA GGCTCTGCAA GTGAGATCGA GATAAATAAG 
GATGCAGTTG TTTGCAGTCT ACAGAAGGGA GAGGAATTAG ATCACACTGG ATTAGTCCAC 
ACACCCCCAG CTGCAGCTAA TGTCATTCTT ACTGAAGAGA GTTACCACAT TCTCCCGGTT 
GTGCAACAAG ATTTGAGCAG GATTTTGAAG AATGACGAAG TGAATACAGT GCGAATGAAA 
GAACGAGGCC AAGATGCACT CGTCCTGAAA AAAGTGTCGT CAGCTAGCCC AGCCCCCACA 
GCAGGGAGTG CCCAGAATGA CTGGAGGTAT GTGGTGGTGT CTGGGACCCC CGAGAAGATT 
CTCGAGCACC TCCTGAATGA TCTACATCTG GAGGAAGGTG AATGCAAAGA GACAGATATC 
TTGCTGGATG ATTTTCTCCT CACTTACACA GTCTTCATGT CAACCAATGA CTTAAGCCAA 
GCATTACTGA GGCACTATTG TTCCAAGCCC TATGCAGAAA GGCCAGGGCA CACAGACAAT 
GGCTTGTACC AAAAGCGCAA AGTGCTGAGA ATTGTGTCGC AATGGGTATC TCTTTACAAG 
AATTTATTGC GAGAAGATGA AAATACTAAA TTGCTTTTGA AGAACCTTTA TAAATGCGTG 
CTTGACGATC TTTTTGAGTA TCCTGGTTTG GAGAAAGAGC TGAAGGAGCT TCAAAACCTA 
TTCCGGATCC CTCGCAGACA TACCATTGAT GAATATTCAC CCCACAAAAA GAACGCAACA 
GTTTTTCACC AACTCAGCTG CAGAGAGAAA TGGCTTCATC CAAAAGGCTG TCCAGGTGAA 
TCTGACGAAG TTTTCTGCCG TGTTTACGTG GCAGATCATT CCTATGTCAG CTTCTGGACT 
AAGGAGTCAA CCTCAGCACA GGAGGTCCTT CACGCTGTAG CTGAAAAGCT CCAGAAACCC 
GAGCAGGATT TGATACTGGT GGCTATCACG TCCTCGGAGG AAAGACAAAT CCTTCAGCCC 
GATGATGTCT CCATTTTCAA ATCTCTTGAT GCCACTACTC GTATATTTGT TTGCAGAAAA 
GATCCCAGCG ATTTCCTGAC TTTTATAGCA GAAGATGATG AAGCACCACA CAGGAGCATC 
CGAATGCTTG GAATGAACAC GTGGCACATG GCCAATGAAC TCACCAGCTT TGATTGGGAC 
TTGTTTAACT GTATCTGTGA GCAGGAGCTG ATTTGCTACA TTTTCCGCAG ACAGGACAGT 
GGGAGGAGTA CAGTCAATCT GAACCTGCTG CTGCAGCGAT GTAATGAAGT CCAGTTCTGG 
GTCGCAACTG AAATCCTCCT GTGCAGCCAG CTGTCCAAAA GAGTTCAGCT GGTCAAAAAG 
TTCATAAAGA TCGCTGCACA CTGCAGGGCA TTGCGGAACC TTAACTCCTT CTTCGCCATT 
ATCATGGGTC TGAACAGCGC TGCTGTCTGC CGACTGGGAC AAACATGGGA GAAAGTGCCT 
GGGAAATTTA AAAGGCTTTT CTCTGAACTT GAAAATTTAA CAGACCCGTC ACTGAACCAT 
AAAGCATACA GAGACGCCTT CAAGAAAATG CAAGCACCAA AGATTCCCTT TATGCCTCTG 
CTACTAAAAG ATGTGACGTT TATCCACGAA GGAAACAAAA CTTTTTTGGA CAATCTTGTC 
AACTTTCAAA AACTGCACAT GATTGCCGAC ACCGTCAGAT TGCTGAGACA CTGCAGGAGT 
AACCAGAGCA GCACAGAGGG AACGTATAAA GAGCACCAGG ATGTGAAGAC TTACATCCAG 
TACCTGCACA TTATCGACAA CCAGCAGATG CTGTTTCAGC TTTCCCACAG ATTGGAGCCA 
AGGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007901510.1
CDS	143..2791
Translation	MAQQNDIPGSLCVPEVGLLAALKWSRNAKEQGHLKQRIKDLPSLLKNGFNFRKKQADMQMASSEKNAESHPSNSNRKIYSETIYCAGRALRDFFIILAPHLINDRVTSTGKYKRSCVGFEMVDWLQDQCLFILNRANAESIWQILLELGILSSATGKQQNFQDEDAYYQFSRDECDVPACPFGKEEEWKNGVRLLAHMVPYVQSRTTMLTSERESEDEKAEPCDEVLQLQALALLSATVKKELAAVIASKGGTSLCDQEDERVLNQQDMKGSASEIEINKDAVVCSLQKGEELDHTGLVHTPPAAANVILTEESYHILPVVQQDLSRILKNDEVNTVRMKERGQDALVLKKVSSASPAPTAGSAQNDWRYVVVSGTPEKILEHLLNDLHLEEGECKETDILLDDFLLTYTVFMSTNDLSQALLRHYCSKPYAERPGHTDNGLYQKRKVLRIVSQWVSLYKNLLREDENTKLLLKNLYKCVLDDLFEYPGLEKELKELQNLFRIPRRHTIDEYSPHKKNATVFHQLSCREKWLHPKGCPGESDEVFCRVYVADHSYVSFWTKESTSAQEVLHAVAEKLQKPEQDLILVAITSSEERQILQPDDVSIFKSLDATTRIFVCRKDPSDFLTFIAEDDEAPHRSIRMLGMNTWHMANELTSFDWDLFNCICEQELICYIFRRQDSGRSTVNLNLLLQRCNEVQFWVATEILLCSQLSKRVQLVKKFIKIAAHCRALRNLNSFFAIIMGLNSAAVCRLGQTWEKVPGKFKRLFSELENLTDPSLNHKAYRDAFKKMQAPKIPFMPLLLKDVTFIHEGNKTFLDNLVNFQKLHMIADTVRLLRHCRSNQSSTEGTYKEHQDVKTYIQYLHIIDNQQMLFQLSHRLEPRT

Target ORF information:

RefSeq Version	XM_007903319.1
Organism	Callorhinchus milii(elephant shark)
Definition	Callorhinchus milii Rap guanine nucleotide exchange factor (GEF) 5 (rapgef5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007903319.1

ORF Insert Sequence:

ATGGCTCAGC AGAATGATAT TCCTGGCAGT CTCTGTGTCC CAGAAGTGGG ACTTTTAGCT 
GCTTTAAAAT GGAGCAGAAA TGCAAAGGAA CAAGGACATT TAAAACAGAG GATAAAAGAC 
CTTCCTTCAC TACTAAAAAA TGGATTTAAT TTCAGGAAGA AGCAGGCAGA TATGCAGATG 
GCCAGTTCAG AAAAGAATGC AGAATCGCAC CCATCCAATT CCAACAGAAA GATTTATTCT 
GAGACCATCT ACTGTGCTGG GCGAGCCCTT AGAGACTTCT TCATAATCCT TGCTCCTCAT 
CTCATAAACG ATCGAGTAAC CAGTACAGGA AAATACAAGA GAAGCTGTGT GGGGTTTGAG 
ATGGTGGACT GGCTGCAGGA CCAGTGTCTG TTCATCCTCA ACCGGGCGAA TGCAGAGAGT 
ATCTGGCAGA TTCTCCTGGA GCTGGGAATT CTATCTTCAG CAACGGGAAA GCAACAGAAT 
TTTCAGGACG AAGATGCTTA TTACCAGTTT TCGAGGGATG AATGTGATGT CCCTGCCTGC 
CCTTTTGGAA AGGAGGAAGA GTGGAAAAAC GGTGTGAGAC TTCTTGCTCA CATGGTCCCT 
TATGTCCAGT CCAGGACAAC AATGTTAACA TCAGAACGAG AATCAGAAGA TGAGAAAGCA 
GAACCGTGTG ATGAAGTTCT CCAGCTTCAA GCCTTGGCTC TCTTATCAGC AACGGTGAAG 
AAGGAGCTGG CTGCTGTTAT TGCTTCAAAA GGAGGAACAT CATTGTGCGA CCAGGAGGAT 
GAAAGAGTTC TCAACCAACA AGATATGAAA GGCTCTGCAA GTGAGATCGA GATAAATAAG 
GATGCAGTTG TTTGCAGTCT ACAGAAGGGA GAGGAATTAG ATCACACTGG ATTAGTCCAC 
ACACCCCCAG CTGCAGCTAA TGTCATTCTT ACTGAAGAGA GTTACCACAT TCTCCCGGTT 
GTGCAACAAG ATTTGAGCAG GATTTTGAAG AATGACGAAG TGAATACAGT GCGAATGAAA 
GAACGAGGCC AAGATGCACT CGTCCTGAAA AAAGTGTCGT CAGCTAGCCC AGCCCCCACA 
GCAGGGAGTG CCCAGAATGA CTGGAGGTAT GTGGTGGTGT CTGGGACCCC CGAGAAGATT 
CTCGAGCACC TCCTGAATGA TCTACATCTG GAGGAAGGTG AATGCAAAGA GACAGATATC 
TTGCTGGATG ATTTTCTCCT CACTTACACA GTCTTCATGT CAACCAATGA CTTAAGCCAA 
GCATTACTGA GGCACTATTG TTCCAAGCCC TATGCAGAAA GGCCAGGGCA CACAGACAAT 
GGCTTGTACC AAAAGCGCAA AGTGCTGAGA ATTGTGTCGC AATGGGTATC TCTTTACAAG 
AATTTATTGC GAGAAGATGA AAATACTAAA TTGCTTTTGA AGAACCTTTA TAAATGCGTG 
CTTGACGATC TTTTTGAGTA TCCTGGTTTG GAGAAAGAGC TGAAGGAGCT TCAAAACCTA 
TTCCGGATCC CTCGCAGACA TACCATTGAT GAATATTCAC CCCACAAAAA GAACGCAACA 
GTTTTTCACC AACTCAGCTG CAGAGAGAAA TGGCTTCATC CAAAAGGCTG TCCAGGTGAA 
TCTGACGAAG TTTTCTGCCG TGTTTACGTG GCAGATCATT CCTATGTCAG CTTCTGGACT 
AAGGAGTCAA CCTCAGCACA GGAGGTCCTT CACGCTGTAG CTGAAAAGCT CCAGAAACCC 
GAGCAGGATT TGATACTGGT GGCTATCACG TCCTCGGAGG AAAGACAAAT CCTTCAGCCC 
GATGATGTCT CCATTTTCAA ATCTCTTGAT GCCACTACTC GTATATTTGT TTGCAGAAAA 
GATCCCAGCG ATTTCCTGAC TTTTATAGCA GAAGATGATG AAGCACCACA CAGGAGCATC 
CGAATGCTTG GAATGAACAC GTGGCACATG GCCAATGAAC TCACCAGCTT TGATTGGGAC 
TTGTTTAACT GTATCTGTGA GCAGGAGCTG ATTTGCTACA TTTTCCGCAG ACAGGACAGT 
GGGAGGAGTA CAGTCAATCT GAACCTGCTG CTGCAGCGAT GTAATGAAGT CCAGTTCTGG 
GTCGCAACTG AAATCCTCCT GTGCAGCCAG CTGTCCAAAA GAGTTCAGCT GGTCAAAAAG 
TTCATAAAGA TCGCTGCACA CTGCAGGGCA TTGCGGAACC TTAACTCCTT CTTCGCCATT 
ATCATGGGTC TGAACAGCGC TGCTGTCTGC CGACTGGGAC AAACATGGGA GAAAGTGCCT 
GGGAAATTTA AAAGGCTTTT CTCTGAACTT GAAAATTTAA CAGACCCGTC ACTGAACCAT 
AAAGCATACA GAGACGCCTT CAAGAAAATG CAAGCACCAA AGATTCCCTT TATGCCTCTG 
CTACTAAAAG ATGTGACGTT TATCCACGAA GGAAACAAAA CTTTTTTGGA CAATCTTGTC 
AACTTTCAAA AACTGCACAT GATTGCCGAC ACCGTCAGAT TGCTGAGACA CTGCAGGAGT 
AACCAGAGCA GCACAGAGGG AACGTATAAA GAGCACCAGG ATGTGAAGAC TTACATCCAG 
TACCTGCACA TTATCGACAA CCAGCAGATG CTGTTTCAGC TTTCCCACAG ATTGGAGCCA 
AGGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef5 ( XM_007903323.1 ) cDNA ORF clone, Callorhinchus milii(elephant shark) -> XP_007901514.1 Callorhinchus milii Rap guanine nucleotide exchange factor (GEF) 5 (rapgef5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa370589
Clone ID Related Accession (Same CDS sequence)	XM_007903323.1
Accession Version	XM_007903323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-08
Organism	Callorhinchus milii(elephant shark)
Product	rap guanine nucleotide exchange factor 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006890057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callorhinchus milii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGC AGAATGATAT TCCTGGCAGT CTCTGTGTCC CAGAAGTGGG ACTTTTAGCT 
GCTTTAAAAT GGAGCAGAAA TGCAAAGGAA CAAGGACATT TAAAACAGAG GATAAAAGAC 
CTTCCTTCAC TACTAAAAAA TGGATTTAAT TTCAGGAAGA AGCAGGCAGA TATGATGGCC 
AGTTCAGAAA AGAATGCAGA ATCGCACCCA TCCAATTCCA ACAGAAAGAT TTATTCTGAG 
ACCATCTACT GTGCTGGGCG AGCCCTTAGA GACTTCTTCA TAATCCTTGC TCCTCATCTC 
ATAAACGATC GAGTAACCAG TACAGGAAAA TACAAGAGAA GCTGTGTGGG GTTTGAGATG 
GTGGACTGGC TGCAGGACCA GTGTCTGTTC ATCCTCAACC GGGCGAATGC AGAGAGTATC 
TGGCAGATTC TCCTGGAGCT GGGAATTCTA TCTTCAGCAA CGGGAAAGCA ACAGAATTTT 
CAGGACGAAG ATGCTTATTA CCAGTTTTCG AGGGATGAAT GTGATGTCCC TGCCTGCCCT 
TTTGGAAAGG AGGAAGAGTG GAAAAACGGT GTGAGACTTC TTGCTCACAT GGTCCCTTAT 
GTCCAGTCCA GGACAACAAT GTTAACATCA GAACGAGAAT CAGAAGATGA GAAAGCAGAA 
CCGTGTGATG AAGTTCTCCA GCTTCAAGCC TTGGCTCTCT TATCAGCAAC GGTGAAGAAG 
GAGCTGGCTG CTGTTATTGC TTCAAAAGGA GGAACATCAT TGTGCGACCA GGAGGATGAA 
AGAGTTCTCA ACCAACAAGA TATGAAAGGC TCTGCAAGTG AGATCGAGAT AAATAAGGAT 
GCAGTTGTTT GCAGTCTACA GAAGGGAGAG GAATTAGATC ACACTGGATT AGTCCACACA 
CCCCCAGCTG CAGCTAATGT CATTCTTACT GAAGAGAGTT ACCACATTCT CCCGGTTGTG 
CAACAAGATT TGAGCAGGAT TTTGAAGAAT GACGAAGTGA ATACAGTGCG AATGAAAGAA 
CGAGGCCAAG ATGCACTCGT CCTGAAAAAA GTGTCGTCAG CTAGCCCAGC CCCCACAGCA 
GGGAGTGCCC AGAATGACTG GAGGTATGTG GTGGTGTCTG GGACCCCCGA GAAGATTCTC 
GAGCACCTCC TGAATGATCT ACATCTGGAG GAAGGTGAAT GCAAAGAGAC AGATATCTTG 
CTGGATGATT TTCTCCTCAC TTACACAGTC TTCATGTCAA CCAATGACTT AAGCCAAGCA 
TTACTGAGGC ACTATTGTTC CAAGCCCTAT GCAGAAAGGC CAGGGCACAC AGACAATGGC 
TTGTACCAAA AGCGCAAAGT GCTGAGAATT GTGTCGCAAT GGGTATCTCT TTACAAGAAT 
TTATTGCGAG AAGATGAAAA TACTAAATTG CTTTTGAAGA ACCTTTATAA ATGCGTGCTT 
GACGATCTTT TTGAGTATCC TGGTTTGGAG AAAGAGCTGA AGGAGCTTCA AAACCTATTC 
CGGATCCCTC GCAGACATAC CATTGATGAA TATTCACCCC ACAAAAAGAA CGCAACAGTT 
TTTCACCAAC TCAGCTGCAG AGAGAAATGG CTTCATCCAA AAGGCTGTCC AGGTGAATCT 
GACGAAGTTT TCTGCCGTGT TTACGTGGCA GATCATTCCT ATGTCAGCTT CTGGACTAAG 
GAGTCAACCT CAGCACAGGA GGTCCTTCAC GCTGTAGCTG AAAAGCTCCA GAAACCCGAG 
CAGGATTTGA TACTGGTGGC TATCACGTCC TCGGAGGAAA GACAAATCCT TCAGCCCGAT 
GATGTCTCCA TTTTCAAATC TCTTGATGCC ACTACTCGTA TATTTGTTTG CAGAAAAGAT 
CCCAGCGATT TCCTGACTTT TATAGCAGAA GATGATGAAG CACCACACAG GAGCATCCGA 
ATGCTTGGAA TGAACACGTG GCACATGGCC AATGAACTCA CCAGCTTTGA TTGGGACTTG 
TTTAACTGTA TCTGTGAGCA GGAGCTGATT TGCTACATTT TCCGCAGACA GGACAGTGGG 
AGGAGTACAG TCAATCTGAA CCTGCTGCTG CAGCGATGTA ATGAAGTCCA GTTCTGGGTC 
GCAACTGAAA TCCTCCTGTG CAGCCAGCTG TCCAAAAGAG TTCAGCTGGT CAAAAAGTTC 
ATAAAGATCG CTGCACACTG CAGGGCATTG CGGAACCTTA ACTCCTTCTT CGCCATTATC 
ATGGGTCTGA ACAGCGCTGC TGTCTGCCGA CTGGGACAAA CATGGGAGAA AGTGCCTGGG 
AAATTTAAAA GGCTTTTCTC TGAACTTGAA AATTTAACAG ACCCGTCACT GAACCATAAA 
GCATACAGAG ACGCCTTCAA GAAAATGCAA GCACCAAAGA TTCCCTTTAT GCCTCTGCTA 
CTAAAAGATG TGACGTTTAT CCACGAAGGA AACAAAACTT TTTTGGACAA TCTTGTCAAC 
TTTCAAAAAC TGCACATGAT TGCCGACACC GTCAGATTGC TGAGACACTG CAGGAGTAAC 
CAGAGCAGCA CAGAGGGAAC GTATAAAGAG CACCAGGATG TGAAGACTTA CATCCAGTAC 
CTGCACATTA TCGACAACCA GCAGATGCTG TTTCAGCTTT CCCACAGATT GGAGCCAAGG 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007901514.1
CDS	143..2788
Translation	MAQQNDIPGSLCVPEVGLLAALKWSRNAKEQGHLKQRIKDLPSLLKNGFNFRKKQADMMASSEKNAESHPSNSNRKIYSETIYCAGRALRDFFIILAPHLINDRVTSTGKYKRSCVGFEMVDWLQDQCLFILNRANAESIWQILLELGILSSATGKQQNFQDEDAYYQFSRDECDVPACPFGKEEEWKNGVRLLAHMVPYVQSRTTMLTSERESEDEKAEPCDEVLQLQALALLSATVKKELAAVIASKGGTSLCDQEDERVLNQQDMKGSASEIEINKDAVVCSLQKGEELDHTGLVHTPPAAANVILTEESYHILPVVQQDLSRILKNDEVNTVRMKERGQDALVLKKVSSASPAPTAGSAQNDWRYVVVSGTPEKILEHLLNDLHLEEGECKETDILLDDFLLTYTVFMSTNDLSQALLRHYCSKPYAERPGHTDNGLYQKRKVLRIVSQWVSLYKNLLREDENTKLLLKNLYKCVLDDLFEYPGLEKELKELQNLFRIPRRHTIDEYSPHKKNATVFHQLSCREKWLHPKGCPGESDEVFCRVYVADHSYVSFWTKESTSAQEVLHAVAEKLQKPEQDLILVAITSSEERQILQPDDVSIFKSLDATTRIFVCRKDPSDFLTFIAEDDEAPHRSIRMLGMNTWHMANELTSFDWDLFNCICEQELICYIFRRQDSGRSTVNLNLLLQRCNEVQFWVATEILLCSQLSKRVQLVKKFIKIAAHCRALRNLNSFFAIIMGLNSAAVCRLGQTWEKVPGKFKRLFSELENLTDPSLNHKAYRDAFKKMQAPKIPFMPLLLKDVTFIHEGNKTFLDNLVNFQKLHMIADTVRLLRHCRSNQSSTEGTYKEHQDVKTYIQYLHIIDNQQMLFQLSHRLEPRT

Target ORF information:

RefSeq Version	XM_007903323.1
Organism	Callorhinchus milii(elephant shark)
Definition	Callorhinchus milii Rap guanine nucleotide exchange factor (GEF) 5 (rapgef5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007903323.1

ORF Insert Sequence:

ATGGCTCAGC AGAATGATAT TCCTGGCAGT CTCTGTGTCC CAGAAGTGGG ACTTTTAGCT 
GCTTTAAAAT GGAGCAGAAA TGCAAAGGAA CAAGGACATT TAAAACAGAG GATAAAAGAC 
CTTCCTTCAC TACTAAAAAA TGGATTTAAT TTCAGGAAGA AGCAGGCAGA TATGATGGCC 
AGTTCAGAAA AGAATGCAGA ATCGCACCCA TCCAATTCCA ACAGAAAGAT TTATTCTGAG 
ACCATCTACT GTGCTGGGCG AGCCCTTAGA GACTTCTTCA TAATCCTTGC TCCTCATCTC 
ATAAACGATC GAGTAACCAG TACAGGAAAA TACAAGAGAA GCTGTGTGGG GTTTGAGATG 
GTGGACTGGC TGCAGGACCA GTGTCTGTTC ATCCTCAACC GGGCGAATGC AGAGAGTATC 
TGGCAGATTC TCCTGGAGCT GGGAATTCTA TCTTCAGCAA CGGGAAAGCA ACAGAATTTT 
CAGGACGAAG ATGCTTATTA CCAGTTTTCG AGGGATGAAT GTGATGTCCC TGCCTGCCCT 
TTTGGAAAGG AGGAAGAGTG GAAAAACGGT GTGAGACTTC TTGCTCACAT GGTCCCTTAT 
GTCCAGTCCA GGACAACAAT GTTAACATCA GAACGAGAAT CAGAAGATGA GAAAGCAGAA 
CCGTGTGATG AAGTTCTCCA GCTTCAAGCC TTGGCTCTCT TATCAGCAAC GGTGAAGAAG 
GAGCTGGCTG CTGTTATTGC TTCAAAAGGA GGAACATCAT TGTGCGACCA GGAGGATGAA 
AGAGTTCTCA ACCAACAAGA TATGAAAGGC TCTGCAAGTG AGATCGAGAT AAATAAGGAT 
GCAGTTGTTT GCAGTCTACA GAAGGGAGAG GAATTAGATC ACACTGGATT AGTCCACACA 
CCCCCAGCTG CAGCTAATGT CATTCTTACT GAAGAGAGTT ACCACATTCT CCCGGTTGTG 
CAACAAGATT TGAGCAGGAT TTTGAAGAAT GACGAAGTGA ATACAGTGCG AATGAAAGAA 
CGAGGCCAAG ATGCACTCGT CCTGAAAAAA GTGTCGTCAG CTAGCCCAGC CCCCACAGCA 
GGGAGTGCCC AGAATGACTG GAGGTATGTG GTGGTGTCTG GGACCCCCGA GAAGATTCTC 
GAGCACCTCC TGAATGATCT ACATCTGGAG GAAGGTGAAT GCAAAGAGAC AGATATCTTG 
CTGGATGATT TTCTCCTCAC TTACACAGTC TTCATGTCAA CCAATGACTT AAGCCAAGCA 
TTACTGAGGC ACTATTGTTC CAAGCCCTAT GCAGAAAGGC CAGGGCACAC AGACAATGGC 
TTGTACCAAA AGCGCAAAGT GCTGAGAATT GTGTCGCAAT GGGTATCTCT TTACAAGAAT 
TTATTGCGAG AAGATGAAAA TACTAAATTG CTTTTGAAGA ACCTTTATAA ATGCGTGCTT 
GACGATCTTT TTGAGTATCC TGGTTTGGAG AAAGAGCTGA AGGAGCTTCA AAACCTATTC 
CGGATCCCTC GCAGACATAC CATTGATGAA TATTCACCCC ACAAAAAGAA CGCAACAGTT 
TTTCACCAAC TCAGCTGCAG AGAGAAATGG CTTCATCCAA AAGGCTGTCC AGGTGAATCT 
GACGAAGTTT TCTGCCGTGT TTACGTGGCA GATCATTCCT ATGTCAGCTT CTGGACTAAG 
GAGTCAACCT CAGCACAGGA GGTCCTTCAC GCTGTAGCTG AAAAGCTCCA GAAACCCGAG 
CAGGATTTGA TACTGGTGGC TATCACGTCC TCGGAGGAAA GACAAATCCT TCAGCCCGAT 
GATGTCTCCA TTTTCAAATC TCTTGATGCC ACTACTCGTA TATTTGTTTG CAGAAAAGAT 
CCCAGCGATT TCCTGACTTT TATAGCAGAA GATGATGAAG CACCACACAG GAGCATCCGA 
ATGCTTGGAA TGAACACGTG GCACATGGCC AATGAACTCA CCAGCTTTGA TTGGGACTTG 
TTTAACTGTA TCTGTGAGCA GGAGCTGATT TGCTACATTT TCCGCAGACA GGACAGTGGG 
AGGAGTACAG TCAATCTGAA CCTGCTGCTG CAGCGATGTA ATGAAGTCCA GTTCTGGGTC 
GCAACTGAAA TCCTCCTGTG CAGCCAGCTG TCCAAAAGAG TTCAGCTGGT CAAAAAGTTC 
ATAAAGATCG CTGCACACTG CAGGGCATTG CGGAACCTTA ACTCCTTCTT CGCCATTATC 
ATGGGTCTGA ACAGCGCTGC TGTCTGCCGA CTGGGACAAA CATGGGAGAA AGTGCCTGGG 
AAATTTAAAA GGCTTTTCTC TGAACTTGAA AATTTAACAG ACCCGTCACT GAACCATAAA 
GCATACAGAG ACGCCTTCAA GAAAATGCAA GCACCAAAGA TTCCCTTTAT GCCTCTGCTA 
CTAAAAGATG TGACGTTTAT CCACGAAGGA AACAAAACTT TTTTGGACAA TCTTGTCAAC 
TTTCAAAAAC TGCACATGAT TGCCGACACC GTCAGATTGC TGAGACACTG CAGGAGTAAC 
CAGAGCAGCA CAGAGGGAAC GTATAAAGAG CACCAGGATG TGAAGACTTA CATCCAGTAC 
CTGCACATTA TCGACAACCA GCAGATGCTG TTTCAGCTTT CCCACAGATT GGAGCCAAGG 
ACCTAA