nsy-1 cDNA ORF clone, Caenorhabditis elegans

The following nsy-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nsy-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa339180 NM_001306584.2
Latest version!
Caenorhabditis elegans Mitogen-activated protein kinase kinase kinase nsy-1 (nsy-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCa339547 NM_001313625.2
Latest version!
Caenorhabditis elegans Protein kinase domain-containing protein (nsy-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
View Old Accession Versions >>
OCa339547 NM_001313625.1 Caenorhabditis elegans Mitogen-activated protein kinase kinase kinase nsy-1 (nsy-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCa339180 NM_001306584.1 Caenorhabditis elegans Mitogen-activated protein kinase kinase kinase nsy-1 (nsy-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa339180
    Clone ID Related Accession (Same CDS sequence) NM_001306584.1 , NM_001306584.2
    Accession Version NM_001306584.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Mitogen-activated protein kinase kinase kinase nsy-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGCAGA ACAACAAGAG GCAAGTGCAG CATAATCACG AGATGTCTAA TGATGTGTGT 
    CCTTTGCCAC TACCGCCACG TGGCGCTCCG CCTCCAACCG CATACCACGC AAGTCGAATG
    GCTGCAACTT CATCCTCAAC CAACGGATCT TTCGAAAAGT CAGCGATTCC AGGAAATGCA
    CGAAAACTTC ACGTGGTGAT TGTGATTGAT CAGAAAGTGC AAAAGAATTT ACGCGTTCGA
    GAAATGGCTC TGAAAGATGT ACAGAAAGTG GCAGACACAT TGAATGTTAA TTTAACGCGC
    ATCGATTTTG ACAAATTGGA CTTTGGCGAA ACAGAAACTC TGGATCTATT CTACAATGCT
    GACGTGGCAC TTGTTGATGT TACGGTAACC CATCAACAAC CAAGTCTTTG TTATCATATT
    GGAGTTCGTG AGAGCATGGG ACAGTCATAC AATATGATAC TCACTTATTG GTCTCCTGAT
    CCAGAATATC ATATTATGGA TGCTTTGAAG AAAACTCATG CACATCTTCC AATGATTGTA
    TATATTCATC ATCAAGATTC AAATCAATTG CAGAGTTATG ATAAGAATAA TAACGATGAT
    GATTCAAAAC CTCCATTTGC TAGAACAAAT GTTCCAGCAA AAACAATAAC TTTTCAACAT
    CGAATGAAAC AAGTTTTGAA AAGTGTTCAA GTGGAAGCAT CAGCACATTC TCGCGAGAAG
    TTCATGTCGG ATTTGCGAAA AGCTCGTGAA ATCACAGATG GAGATCAGAA GAATGATTAT
    CTTGATAAAA TGAGAACAAG ACTTGATAAT CCGGATGTAT TACATCCAGA TACCGTATCC
    CTTATGATGC TCTCTTATCG TGATAATCAG AATTATGGAG GAATGATTCG TCTTGTTGAT
    GATTTGAAAA GAATTCCGGA TTGTTTGAAA GTTGTAGACA CGCCGGTTAT TCGATATCAG
    TATGCGTTTG CTCTGAACCG AAGAAACAAG GATGGGGACC GTGATTTGGC ATTAAATACC
    GTACTCAGTT TGGTGGAGGG AACAACTGAA AACGAAGAGA AGAATGGCCC ATTGAGTCCA
    GACGTTGTTT GTCTTGCTGG CCGTATCTAC AAAGATAAAT TCATTGCATC CAACTACGAA
    GACCGTGAAA GCCTGAACAG TGCAATTGAA TGGTATCGCC GTGCATTCGA AATGTCACCT
    CTGGAATATT CAGGAATAAA TCTGACAACT CTTCTTCGAG CAAGTGGAGA ACACTTTGAG
    AATAATCTCG AAATGCAGCA AATCGCTGTT GTCCTCAACT CGTTGCTCGG ACGGAAAGGG
    GCACTTCAGA ACCTAATGGA ATATTGGGAT GTGGCAACTT ATTTCGAAGT TTCTGTTCTA
    GCAGAGAATT ACCAGAAAGC ATGTGAAGCA GCTTTGATGA TGGTCAAGTT GAAACCTCCA
    GTTTGGTATT TAAAGTCTAC GATGGAAAAT ATCAAATTAA TAAATCGATG TGCAGCAACA
    ATCAGTCCAA TCGAGAAGGA GAAGCAACAA TTCCTTTTCT GGAGTGAATT CTTCATGGAG
    GCAACTGAGG CAGACACTGA TATTAGTTGC CCAAGATACC CAGTACTTAT TCTGGAACTC
    AATAAGGAAT TCACACCTTC TTATCTGACT CTGAATAATG AAGAGGGAAC AGTAATTCTG
    AGTCATGTTC TGGAAAACAG CCAACAGAAA AAGATTCATC AATCCGAGTT GAGAGGTATA
    CATCGATGGC ATTTTGCAAG GAATAATATC AAAGCAGTGA CTGAATCGAA ACGAGATGAT
    CGTCAATTAT TCCTTTATGT TCATGAAAAT TCAGATGATT TCAACCTTCT GTTCCCGACA
    AAGGCTCATT GCAAAAAAGC CTACGATGAC ATGAAATCAA TGGCCGATGT TGCTGATGGA
    AACTACCAAG GAAGAGTACT AAGCAATCCG GATAATGAGA AGATCAGATT CGAGTATGAA
    TTGAGTAATT CAAATGAGCG TGTCGTTCTT GGAAAAGGAA CCTATGGAAC TGTGTACGCT
    GCACGAGATA TGGATACTCA GCGACAAATT GTTGTAAAAG AAATTGAAGT GAAGTATGAT
    GAGGAAGTAC AACCGTTGAT GGAAGAAATA AGTCTTCATT CCACGTTGTG CCATGCTAAT
    ATTGTACAAT ATCTTGGATG TGATTTGGTT GGAAAAGATG GCTCAAATGA TCACTTTTTG
    ATTTTTATGG AGCATGTTCC TGGAGGATCA CTCAGTTCAT TGCTTCGTTC CAAGTGGGGT
    CCAATGAATG AAAATGCGAT GAACTACTAC GGGAAGCAAA TTCTGGAGGG TCTTAAGTAT
    CTTCACGAGC TAAAAATTGT GCATCGAGAC ATCAAAGGAG ACAATGTTCT TGTGAACACT
    TACTCGGGAG TCTGCAAAAT TTCCGATTTT GGAACATGCA AACGTCTTGC TGGGCTGAAT
    CCAGTAACCG AAACTTTCAC TGGAACTCTT CAGTATATGG CTCCAGAAGT TATCGATCAT
    GGACAACGTG GATATGGTGC ACCTGCAGAT ATTTGGTCAT TTGGGTGTAC AATGGTTGAA
    ATGGCAACTG GGCGGCCTCC GTTTGTTGAG ATGCAGAATC CGCAGGCGGC AATGTTCAGA
    GTTGGAATGT TCAAAACTCA TCCTCCAATT CCAACGGAAA TTACTGAAAA GTGTAGAAAC
    TTTATCAAAA GTTGTTTCTT GCCAGAAGCA TGTGATCGTC CATCAGCAAA AGATCTTCTC
    CAAGATCCAT TTATTTATCA CAACCATCAT TCTATTTCAA GAACGCGTAG CGGCTCGATC
    AACAAGAAAC CTGCCACCAA AATCGAGTTG AATCATGACA AGGAAAAGAA GGAAAAATCG
    AAAAATCAAC GAGAAATGCT CCGTTCAACA TCGCATATCG GTGGAATGGG AGTTGTTGAA
    AGATCTCCAC CAACACCTGA ACCAATGTCA GCCACTCTTA CTGCTGGATT CTCTCATGTT
    CACAGTCAAA CTGTATCGAA TGCATTGTCA ACTGCACGAG AAGAAAAGAA GCTTCATCTC
    AAGATTGATC ATGCAAGGAA TCGAACATTC AGTTCAAGTA GTCCAGTACC AGATGGTCAA
    TCATCTGCTG GAACTAATAT GTCTCATCCT GGATTCCAAT TGAGTCAGCC ATCAAGTCCT
    ATTGTCGATG ATACAAATCA CCCTCATTTG ATTGTCTCTC CAATTTCTCT GAATACAATG
    GGATCTCCAC TTTCATCAGC TGCACTTCTC AATCGAACAA TTTCCGATGA AAGTTCCAAT
    AGTTCAAGTC GATTCTTCAT GCTTCAAAAG GATTCAGAAC GTAGAAGATC CTTGGGACAA
    TTCATGCAAG ATTACAAAGA CTTGATCATT GACTCTTGGA GCACATTGCT CATTAAACAA
    AGTGACACGG AACTCGTAGT GACTGTCTAT ATGTTGGAAA TGTTACTTGA TGGAATGAGA
    GATTTCTTGT TGAAAAAGGA TAATACAAAG ATGCAAAAAA TGATTGATGA TATTCGAGGT
    CTTTTGGATT ATGATACTGC TAAAATTGGC CAGATAAACC TTGCTCTCTA CCACTTCTCC
    GATTCAATTC AACCTGTTCT CCGTCGCCTT GATATAAAGC CTCATTGGAT GTTTGCACTT
    TCAAATCTAA TCACTTCAGC CGTTCAATGT GCCATTTCAA TTCTAAGTCC AGATCTTTCT
    CTCCTACTTC ATGCTCAAGA TAATCTTCCA TCCACATCAT CAATTGTTGC AATCAGAAAT
    TCCTCACTTT CTGAAGGTGA AGCTCTCATT GAATCTCGAC CTCCAAGTCG TGAAGAACGA
    GTTCGTGAGG ATCGAAAAGA ATTGAGAACT TTACAAGAAG AAAATGAAAT TCTAATTGAA
    CGATTGCTTC AAGTGGAAAG AGAATTAAAT GCTCAACTCA AATCCGGAAT CACCAGAGCC
    AATCGTTTCC GAGATTTTGC CATGTACCGT AACACATATC CACCATTCCG TACTCCACCA
    GTCGCTCATG CTCCCCCAAC TCCTCCATTC AGTGCTTCAT GTGGTGCTCA ACCGTCAGGA
    ACATTTACAA ATCAACCTCC GAGCTTCGCC TCAATAAAAC CAATTGCTCA GAAAATCATA
    ATGCCACCCG GAACCGAGAA TAATTATCAA GTGACACGTG TTCAGGAGGA GCTTGTCAGT
    TGGCTACGTG GATTGGAAAT TGACGAACGA TCGATTGCTC TGATCGCCTC CGAAGCTTAT
    ACAAAGAGCG ATATGATGGA TTTTGTGACA AGAGACGAGC TGCTCAGTAT TGGTGTTGGC
    GGAGGTTCAT CTTGTCGTAT AATGCGTGCA ATTGGTGAAG TTCGTGAACG TCAACGCCGT
    CAGCCAGTCT TCCTGTCTCC AATGCGAAGT CGAGATGATT CTTTGGATGA TTATCATTCA
    AGCAGTGCCG ATGATATGTA CACTGGCGCT GCTGCTGAAA CTTCATCTGG AAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293513.1
    CDS1..4497
    Translation

    Target ORF information:

    RefSeq Version NM_001306584.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Mitogen-activated protein kinase kinase kinase nsy-1 (nsy-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306584.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGCAGA ACAACAAGAG GCAAGTGCAG CATAATCACG AGATGTCTAA TGATGTGTGT 
    CCTTTGCCAC TACCGCCACG TGGCGCTCCG CCTCCAACCG CATACCACGC AAGTCGAATG
    GCTGCAACTT CATCCTCAAC CAACGGATCT TTCGAAAAGT CAGCGATTCC AGGAAATGCA
    CGAAAACTTC ACGTGGTGAT TGTGATTGAT CAGAAAGTGC AAAAGAATTT ACGCGTTCGA
    GAAATGGCTC TGAAAGATGT ACAGAAAGTG GCAGACACAT TGAATGTTAA TTTAACGCGC
    ATCGATTTTG ACAAATTGGA CTTTGGCGAA ACAGAAACTC TGGATCTATT CTACAATGCT
    GACGTGGCAC TTGTTGATGT TACGGTAACC CATCAACAAC CAAGTCTTTG TTATCATATT
    GGAGTTCGTG AGAGCATGGG ACAGTCATAC AATATGATAC TCACTTATTG GTCTCCTGAT
    CCAGAATATC ATATTATGGA TGCTTTGAAG AAAACTCATG CACATCTTCC AATGATTGTA
    TATATTCATC ATCAAGATTC AAATCAATTG CAGAGTTATG ATAAGAATAA TAACGATGAT
    GATTCAAAAC CTCCATTTGC TAGAACAAAT GTTCCAGCAA AAACAATAAC TTTTCAACAT
    CGAATGAAAC AAGTTTTGAA AAGTGTTCAA GTGGAAGCAT CAGCACATTC TCGCGAGAAG
    TTCATGTCGG ATTTGCGAAA AGCTCGTGAA ATCACAGATG GAGATCAGAA GAATGATTAT
    CTTGATAAAA TGAGAACAAG ACTTGATAAT CCGGATGTAT TACATCCAGA TACCGTATCC
    CTTATGATGC TCTCTTATCG TGATAATCAG AATTATGGAG GAATGATTCG TCTTGTTGAT
    GATTTGAAAA GAATTCCGGA TTGTTTGAAA GTTGTAGACA CGCCGGTTAT TCGATATCAG
    TATGCGTTTG CTCTGAACCG AAGAAACAAG GATGGGGACC GTGATTTGGC ATTAAATACC
    GTACTCAGTT TGGTGGAGGG AACAACTGAA AACGAAGAGA AGAATGGCCC ATTGAGTCCA
    GACGTTGTTT GTCTTGCTGG CCGTATCTAC AAAGATAAAT TCATTGCATC CAACTACGAA
    GACCGTGAAA GCCTGAACAG TGCAATTGAA TGGTATCGCC GTGCATTCGA AATGTCACCT
    CTGGAATATT CAGGAATAAA TCTGACAACT CTTCTTCGAG CAAGTGGAGA ACACTTTGAG
    AATAATCTCG AAATGCAGCA AATCGCTGTT GTCCTCAACT CGTTGCTCGG ACGGAAAGGG
    GCACTTCAGA ACCTAATGGA ATATTGGGAT GTGGCAACTT ATTTCGAAGT TTCTGTTCTA
    GCAGAGAATT ACCAGAAAGC ATGTGAAGCA GCTTTGATGA TGGTCAAGTT GAAACCTCCA
    GTTTGGTATT TAAAGTCTAC GATGGAAAAT ATCAAATTAA TAAATCGATG TGCAGCAACA
    ATCAGTCCAA TCGAGAAGGA GAAGCAACAA TTCCTTTTCT GGAGTGAATT CTTCATGGAG
    GCAACTGAGG CAGACACTGA TATTAGTTGC CCAAGATACC CAGTACTTAT TCTGGAACTC
    AATAAGGAAT TCACACCTTC TTATCTGACT CTGAATAATG AAGAGGGAAC AGTAATTCTG
    AGTCATGTTC TGGAAAACAG CCAACAGAAA AAGATTCATC AATCCGAGTT GAGAGGTATA
    CATCGATGGC ATTTTGCAAG GAATAATATC AAAGCAGTGA CTGAATCGAA ACGAGATGAT
    CGTCAATTAT TCCTTTATGT TCATGAAAAT TCAGATGATT TCAACCTTCT GTTCCCGACA
    AAGGCTCATT GCAAAAAAGC CTACGATGAC ATGAAATCAA TGGCCGATGT TGCTGATGGA
    AACTACCAAG GAAGAGTACT AAGCAATCCG GATAATGAGA AGATCAGATT CGAGTATGAA
    TTGAGTAATT CAAATGAGCG TGTCGTTCTT GGAAAAGGAA CCTATGGAAC TGTGTACGCT
    GCACGAGATA TGGATACTCA GCGACAAATT GTTGTAAAAG AAATTGAAGT GAAGTATGAT
    GAGGAAGTAC AACCGTTGAT GGAAGAAATA AGTCTTCATT CCACGTTGTG CCATGCTAAT
    ATTGTACAAT ATCTTGGATG TGATTTGGTT GGAAAAGATG GCTCAAATGA TCACTTTTTG
    ATTTTTATGG AGCATGTTCC TGGAGGATCA CTCAGTTCAT TGCTTCGTTC CAAGTGGGGT
    CCAATGAATG AAAATGCGAT GAACTACTAC GGGAAGCAAA TTCTGGAGGG TCTTAAGTAT
    CTTCACGAGC TAAAAATTGT GCATCGAGAC ATCAAAGGAG ACAATGTTCT TGTGAACACT
    TACTCGGGAG TCTGCAAAAT TTCCGATTTT GGAACATGCA AACGTCTTGC TGGGCTGAAT
    CCAGTAACCG AAACTTTCAC TGGAACTCTT CAGTATATGG CTCCAGAAGT TATCGATCAT
    GGACAACGTG GATATGGTGC ACCTGCAGAT ATTTGGTCAT TTGGGTGTAC AATGGTTGAA
    ATGGCAACTG GGCGGCCTCC GTTTGTTGAG ATGCAGAATC CGCAGGCGGC AATGTTCAGA
    GTTGGAATGT TCAAAACTCA TCCTCCAATT CCAACGGAAA TTACTGAAAA GTGTAGAAAC
    TTTATCAAAA GTTGTTTCTT GCCAGAAGCA TGTGATCGTC CATCAGCAAA AGATCTTCTC
    CAAGATCCAT TTATTTATCA CAACCATCAT TCTATTTCAA GAACGCGTAG CGGCTCGATC
    AACAAGAAAC CTGCCACCAA AATCGAGTTG AATCATGACA AGGAAAAGAA GGAAAAATCG
    AAAAATCAAC GAGAAATGCT CCGTTCAACA TCGCATATCG GTGGAATGGG AGTTGTTGAA
    AGATCTCCAC CAACACCTGA ACCAATGTCA GCCACTCTTA CTGCTGGATT CTCTCATGTT
    CACAGTCAAA CTGTATCGAA TGCATTGTCA ACTGCACGAG AAGAAAAGAA GCTTCATCTC
    AAGATTGATC ATGCAAGGAA TCGAACATTC AGTTCAAGTA GTCCAGTACC AGATGGTCAA
    TCATCTGCTG GAACTAATAT GTCTCATCCT GGATTCCAAT TGAGTCAGCC ATCAAGTCCT
    ATTGTCGATG ATACAAATCA CCCTCATTTG ATTGTCTCTC CAATTTCTCT GAATACAATG
    GGATCTCCAC TTTCATCAGC TGCACTTCTC AATCGAACAA TTTCCGATGA AAGTTCCAAT
    AGTTCAAGTC GATTCTTCAT GCTTCAAAAG GATTCAGAAC GTAGAAGATC CTTGGGACAA
    TTCATGCAAG ATTACAAAGA CTTGATCATT GACTCTTGGA GCACATTGCT CATTAAACAA
    AGTGACACGG AACTCGTAGT GACTGTCTAT ATGTTGGAAA TGTTACTTGA TGGAATGAGA
    GATTTCTTGT TGAAAAAGGA TAATACAAAG ATGCAAAAAA TGATTGATGA TATTCGAGGT
    CTTTTGGATT ATGATACTGC TAAAATTGGC CAGATAAACC TTGCTCTCTA CCACTTCTCC
    GATTCAATTC AACCTGTTCT CCGTCGCCTT GATATAAAGC CTCATTGGAT GTTTGCACTT
    TCAAATCTAA TCACTTCAGC CGTTCAATGT GCCATTTCAA TTCTAAGTCC AGATCTTTCT
    CTCCTACTTC ATGCTCAAGA TAATCTTCCA TCCACATCAT CAATTGTTGC AATCAGAAAT
    TCCTCACTTT CTGAAGGTGA AGCTCTCATT GAATCTCGAC CTCCAAGTCG TGAAGAACGA
    GTTCGTGAGG ATCGAAAAGA ATTGAGAACT TTACAAGAAG AAAATGAAAT TCTAATTGAA
    CGATTGCTTC AAGTGGAAAG AGAATTAAAT GCTCAACTCA AATCCGGAAT CACCAGAGCC
    AATCGTTTCC GAGATTTTGC CATGTACCGT AACACATATC CACCATTCCG TACTCCACCA
    GTCGCTCATG CTCCCCCAAC TCCTCCATTC AGTGCTTCAT GTGGTGCTCA ACCGTCAGGA
    ACATTTACAA ATCAACCTCC GAGCTTCGCC TCAATAAAAC CAATTGCTCA GAAAATCATA
    ATGCCACCCG GAACCGAGAA TAATTATCAA GTGACACGTG TTCAGGAGGA GCTTGTCAGT
    TGGCTACGTG GATTGGAAAT TGACGAACGA TCGATTGCTC TGATCGCCTC CGAAGCTTAT
    ACAAAGAGCG ATATGATGGA TTTTGTGACA AGAGACGAGC TGCTCAGTAT TGGTGTTGGC
    GGAGGTTCAT CTTGTCGTAT AATGCGTGCA ATTGGTGAAG TTCGTGAACG TCAACGCCGT
    CAGCCAGTCT TCCTGTCTCC AATGCGAAGT CGAGATGATT CTTTGGATGA TTATCATTCA
    AGCAGTGCCG ATGATATGTA CACTGGCGCT GCTGCTGAAA CTTCATCTGG AAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa339180
    Clone ID Related Accession (Same CDS sequence) NM_001306584.1 , NM_001306584.2
    Accession Version NM_001306584.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Mitogen-activated protein kinase kinase kinase nsy-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001306584.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGCAGA ACAACAAGAG GCAAGTGCAG CATAATCACG AGATGTCTAA TGATGTGTGT 
    CCTTTGCCAC TACCGCCACG TGGCGCTCCG CCTCCAACCG CATACCACGC AAGTCGAATG
    GCTGCAACTT CATCCTCAAC CAACGGATCT TTCGAAAAGT CAGCGATTCC AGGAAATGCA
    CGAAAACTTC ACGTGGTGAT TGTGATTGAT CAGAAAGTGC AAAAGAATTT ACGCGTTCGA
    GAAATGGCTC TGAAAGATGT ACAGAAAGTG GCAGACACAT TGAATGTTAA TTTAACGCGC
    ATCGATTTTG ACAAATTGGA CTTTGGCGAA ACAGAAACTC TGGATCTATT CTACAATGCT
    GACGTGGCAC TTGTTGATGT TACGGTAACC CATCAACAAC CAAGTCTTTG TTATCATATT
    GGAGTTCGTG AGAGCATGGG ACAGTCATAC AATATGATAC TCACTTATTG GTCTCCTGAT
    CCAGAATATC ATATTATGGA TGCTTTGAAG AAAACTCATG CACATCTTCC AATGATTGTA
    TATATTCATC ATCAAGATTC AAATCAATTG CAGAGTTATG ATAAGAATAA TAACGATGAT
    GATTCAAAAC CTCCATTTGC TAGAACAAAT GTTCCAGCAA AAACAATAAC TTTTCAACAT
    CGAATGAAAC AAGTTTTGAA AAGTGTTCAA GTGGAAGCAT CAGCACATTC TCGCGAGAAG
    TTCATGTCGG ATTTGCGAAA AGCTCGTGAA ATCACAGATG GAGATCAGAA GAATGATTAT
    CTTGATAAAA TGAGAACAAG ACTTGATAAT CCGGATGTAT TACATCCAGA TACCGTATCC
    CTTATGATGC TCTCTTATCG TGATAATCAG AATTATGGAG GAATGATTCG TCTTGTTGAT
    GATTTGAAAA GAATTCCGGA TTGTTTGAAA GTTGTAGACA CGCCGGTTAT TCGATATCAG
    TATGCGTTTG CTCTGAACCG AAGAAACAAG GATGGGGACC GTGATTTGGC ATTAAATACC
    GTACTCAGTT TGGTGGAGGG AACAACTGAA AACGAAGAGA AGAATGGCCC ATTGAGTCCA
    GACGTTGTTT GTCTTGCTGG CCGTATCTAC AAAGATAAAT TCATTGCATC CAACTACGAA
    GACCGTGAAA GCCTGAACAG TGCAATTGAA TGGTATCGCC GTGCATTCGA AATGTCACCT
    CTGGAATATT CAGGAATAAA TCTGACAACT CTTCTTCGAG CAAGTGGAGA ACACTTTGAG
    AATAATCTCG AAATGCAGCA AATCGCTGTT GTCCTCAACT CGTTGCTCGG ACGGAAAGGG
    GCACTTCAGA ACCTAATGGA ATATTGGGAT GTGGCAACTT ATTTCGAAGT TTCTGTTCTA
    GCAGAGAATT ACCAGAAAGC ATGTGAAGCA GCTTTGATGA TGGTCAAGTT GAAACCTCCA
    GTTTGGTATT TAAAGTCTAC GATGGAAAAT ATCAAATTAA TAAATCGATG TGCAGCAACA
    ATCAGTCCAA TCGAGAAGGA GAAGCAACAA TTCCTTTTCT GGAGTGAATT CTTCATGGAG
    GCAACTGAGG CAGACACTGA TATTAGTTGC CCAAGATACC CAGTACTTAT TCTGGAACTC
    AATAAGGAAT TCACACCTTC TTATCTGACT CTGAATAATG AAGAGGGAAC AGTAATTCTG
    AGTCATGTTC TGGAAAACAG CCAACAGAAA AAGATTCATC AATCCGAGTT GAGAGGTATA
    CATCGATGGC ATTTTGCAAG GAATAATATC AAAGCAGTGA CTGAATCGAA ACGAGATGAT
    CGTCAATTAT TCCTTTATGT TCATGAAAAT TCAGATGATT TCAACCTTCT GTTCCCGACA
    AAGGCTCATT GCAAAAAAGC CTACGATGAC ATGAAATCAA TGGCCGATGT TGCTGATGGA
    AACTACCAAG GAAGAGTACT AAGCAATCCG GATAATGAGA AGATCAGATT CGAGTATGAA
    TTGAGTAATT CAAATGAGCG TGTCGTTCTT GGAAAAGGAA CCTATGGAAC TGTGTACGCT
    GCACGAGATA TGGATACTCA GCGACAAATT GTTGTAAAAG AAATTGAAGT GAAGTATGAT
    GAGGAAGTAC AACCGTTGAT GGAAGAAATA AGTCTTCATT CCACGTTGTG CCATGCTAAT
    ATTGTACAAT ATCTTGGATG TGATTTGGTT GGAAAAGATG GCTCAAATGA TCACTTTTTG
    ATTTTTATGG AGCATGTTCC TGGAGGATCA CTCAGTTCAT TGCTTCGTTC CAAGTGGGGT
    CCAATGAATG AAAATGCGAT GAACTACTAC GGGAAGCAAA TTCTGGAGGG TCTTAAGTAT
    CTTCACGAGC TAAAAATTGT GCATCGAGAC ATCAAAGGAG ACAATGTTCT TGTGAACACT
    TACTCGGGAG TCTGCAAAAT TTCCGATTTT GGAACATGCA AACGTCTTGC TGGGCTGAAT
    CCAGTAACCG AAACTTTCAC TGGAACTCTT CAGTATATGG CTCCAGAAGT TATCGATCAT
    GGACAACGTG GATATGGTGC ACCTGCAGAT ATTTGGTCAT TTGGGTGTAC AATGGTTGAA
    ATGGCAACTG GGCGGCCTCC GTTTGTTGAG ATGCAGAATC CGCAGGCGGC AATGTTCAGA
    GTTGGAATGT TCAAAACTCA TCCTCCAATT CCAACGGAAA TTACTGAAAA GTGTAGAAAC
    TTTATCAAAA GTTGTTTCTT GCCAGAAGCA TGTGATCGTC CATCAGCAAA AGATCTTCTC
    CAAGATCCAT TTATTTATCA CAACCATCAT TCTATTTCAA GAACGCGTAG CGGCTCGATC
    AACAAGAAAC CTGCCACCAA AATCGAGTTG AATCATGACA AGGAAAAGAA GGAAAAATCG
    AAAAATCAAC GAGAAATGCT CCGTTCAACA TCGCATATCG GTGGAATGGG AGTTGTTGAA
    AGATCTCCAC CAACACCTGA ACCAATGTCA GCCACTCTTA CTGCTGGATT CTCTCATGTT
    CACAGTCAAA CTGTATCGAA TGCATTGTCA ACTGCACGAG AAGAAAAGAA GCTTCATCTC
    AAGATTGATC ATGCAAGGAA TCGAACATTC AGTTCAAGTA GTCCAGTACC AGATGGTCAA
    TCATCTGCTG GAACTAATAT GTCTCATCCT GGATTCCAAT TGAGTCAGCC ATCAAGTCCT
    ATTGTCGATG ATACAAATCA CCCTCATTTG ATTGTCTCTC CAATTTCTCT GAATACAATG
    GGATCTCCAC TTTCATCAGC TGCACTTCTC AATCGAACAA TTTCCGATGA AAGTTCCAAT
    AGTTCAAGTC GATTCTTCAT GCTTCAAAAG GATTCAGAAC GTAGAAGATC CTTGGGACAA
    TTCATGCAAG ATTACAAAGA CTTGATCATT GACTCTTGGA GCACATTGCT CATTAAACAA
    AGTGACACGG AACTCGTAGT GACTGTCTAT ATGTTGGAAA TGTTACTTGA TGGAATGAGA
    GATTTCTTGT TGAAAAAGGA TAATACAAAG ATGCAAAAAA TGATTGATGA TATTCGAGGT
    CTTTTGGATT ATGATACTGC TAAAATTGGC CAGATAAACC TTGCTCTCTA CCACTTCTCC
    GATTCAATTC AACCTGTTCT CCGTCGCCTT GATATAAAGC CTCATTGGAT GTTTGCACTT
    TCAAATCTAA TCACTTCAGC CGTTCAATGT GCCATTTCAA TTCTAAGTCC AGATCTTTCT
    CTCCTACTTC ATGCTCAAGA TAATCTTCCA TCCACATCAT CAATTGTTGC AATCAGAAAT
    TCCTCACTTT CTGAAGGTGA AGCTCTCATT GAATCTCGAC CTCCAAGTCG TGAAGAACGA
    GTTCGTGAGG ATCGAAAAGA ATTGAGAACT TTACAAGAAG AAAATGAAAT TCTAATTGAA
    CGATTGCTTC AAGTGGAAAG AGAATTAAAT GCTCAACTCA AATCCGGAAT CACCAGAGCC
    AATCGTTTCC GAGATTTTGC CATGTACCGT AACACATATC CACCATTCCG TACTCCACCA
    GTCGCTCATG CTCCCCCAAC TCCTCCATTC AGTGCTTCAT GTGGTGCTCA ACCGTCAGGA
    ACATTTACAA ATCAACCTCC GAGCTTCGCC TCAATAAAAC CAATTGCTCA GAAAATCATA
    ATGCCACCCG GAACCGAGAA TAATTATCAA GTGACACGTG TTCAGGAGGA GCTTGTCAGT
    TGGCTACGTG GATTGGAAAT TGACGAACGA TCGATTGCTC TGATCGCCTC CGAAGCTTAT
    ACAAAGAGCG ATATGATGGA TTTTGTGACA AGAGACGAGC TGCTCAGTAT TGGTGTTGGC
    GGAGGTTCAT CTTGTCGTAT AATGCGTGCA ATTGGTGAAG TTCGTGAACG TCAACGCCGT
    CAGCCAGTCT TCCTGTCTCC AATGCGAAGT CGAGATGATT CTTTGGATGA TTATCATTCA
    AGCAGTGCCG ATGATATGTA CACTGGCGCT GCTGCTGAAA CTTCATCTGG AAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001293513.1
    CDS151..4647
    Translation

    Target ORF information:

    RefSeq Version NM_001306584.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Mitogen-activated protein kinase kinase kinase nsy-1 (nsy-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001306584.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGCAGA ACAACAAGAG GCAAGTGCAG CATAATCACG AGATGTCTAA TGATGTGTGT 
    CCTTTGCCAC TACCGCCACG TGGCGCTCCG CCTCCAACCG CATACCACGC AAGTCGAATG
    GCTGCAACTT CATCCTCAAC CAACGGATCT TTCGAAAAGT CAGCGATTCC AGGAAATGCA
    CGAAAACTTC ACGTGGTGAT TGTGATTGAT CAGAAAGTGC AAAAGAATTT ACGCGTTCGA
    GAAATGGCTC TGAAAGATGT ACAGAAAGTG GCAGACACAT TGAATGTTAA TTTAACGCGC
    ATCGATTTTG ACAAATTGGA CTTTGGCGAA ACAGAAACTC TGGATCTATT CTACAATGCT
    GACGTGGCAC TTGTTGATGT TACGGTAACC CATCAACAAC CAAGTCTTTG TTATCATATT
    GGAGTTCGTG AGAGCATGGG ACAGTCATAC AATATGATAC TCACTTATTG GTCTCCTGAT
    CCAGAATATC ATATTATGGA TGCTTTGAAG AAAACTCATG CACATCTTCC AATGATTGTA
    TATATTCATC ATCAAGATTC AAATCAATTG CAGAGTTATG ATAAGAATAA TAACGATGAT
    GATTCAAAAC CTCCATTTGC TAGAACAAAT GTTCCAGCAA AAACAATAAC TTTTCAACAT
    CGAATGAAAC AAGTTTTGAA AAGTGTTCAA GTGGAAGCAT CAGCACATTC TCGCGAGAAG
    TTCATGTCGG ATTTGCGAAA AGCTCGTGAA ATCACAGATG GAGATCAGAA GAATGATTAT
    CTTGATAAAA TGAGAACAAG ACTTGATAAT CCGGATGTAT TACATCCAGA TACCGTATCC
    CTTATGATGC TCTCTTATCG TGATAATCAG AATTATGGAG GAATGATTCG TCTTGTTGAT
    GATTTGAAAA GAATTCCGGA TTGTTTGAAA GTTGTAGACA CGCCGGTTAT TCGATATCAG
    TATGCGTTTG CTCTGAACCG AAGAAACAAG GATGGGGACC GTGATTTGGC ATTAAATACC
    GTACTCAGTT TGGTGGAGGG AACAACTGAA AACGAAGAGA AGAATGGCCC ATTGAGTCCA
    GACGTTGTTT GTCTTGCTGG CCGTATCTAC AAAGATAAAT TCATTGCATC CAACTACGAA
    GACCGTGAAA GCCTGAACAG TGCAATTGAA TGGTATCGCC GTGCATTCGA AATGTCACCT
    CTGGAATATT CAGGAATAAA TCTGACAACT CTTCTTCGAG CAAGTGGAGA ACACTTTGAG
    AATAATCTCG AAATGCAGCA AATCGCTGTT GTCCTCAACT CGTTGCTCGG ACGGAAAGGG
    GCACTTCAGA ACCTAATGGA ATATTGGGAT GTGGCAACTT ATTTCGAAGT TTCTGTTCTA
    GCAGAGAATT ACCAGAAAGC ATGTGAAGCA GCTTTGATGA TGGTCAAGTT GAAACCTCCA
    GTTTGGTATT TAAAGTCTAC GATGGAAAAT ATCAAATTAA TAAATCGATG TGCAGCAACA
    ATCAGTCCAA TCGAGAAGGA GAAGCAACAA TTCCTTTTCT GGAGTGAATT CTTCATGGAG
    GCAACTGAGG CAGACACTGA TATTAGTTGC CCAAGATACC CAGTACTTAT TCTGGAACTC
    AATAAGGAAT TCACACCTTC TTATCTGACT CTGAATAATG AAGAGGGAAC AGTAATTCTG
    AGTCATGTTC TGGAAAACAG CCAACAGAAA AAGATTCATC AATCCGAGTT GAGAGGTATA
    CATCGATGGC ATTTTGCAAG GAATAATATC AAAGCAGTGA CTGAATCGAA ACGAGATGAT
    CGTCAATTAT TCCTTTATGT TCATGAAAAT TCAGATGATT TCAACCTTCT GTTCCCGACA
    AAGGCTCATT GCAAAAAAGC CTACGATGAC ATGAAATCAA TGGCCGATGT TGCTGATGGA
    AACTACCAAG GAAGAGTACT AAGCAATCCG GATAATGAGA AGATCAGATT CGAGTATGAA
    TTGAGTAATT CAAATGAGCG TGTCGTTCTT GGAAAAGGAA CCTATGGAAC TGTGTACGCT
    GCACGAGATA TGGATACTCA GCGACAAATT GTTGTAAAAG AAATTGAAGT GAAGTATGAT
    GAGGAAGTAC AACCGTTGAT GGAAGAAATA AGTCTTCATT CCACGTTGTG CCATGCTAAT
    ATTGTACAAT ATCTTGGATG TGATTTGGTT GGAAAAGATG GCTCAAATGA TCACTTTTTG
    ATTTTTATGG AGCATGTTCC TGGAGGATCA CTCAGTTCAT TGCTTCGTTC CAAGTGGGGT
    CCAATGAATG AAAATGCGAT GAACTACTAC GGGAAGCAAA TTCTGGAGGG TCTTAAGTAT
    CTTCACGAGC TAAAAATTGT GCATCGAGAC ATCAAAGGAG ACAATGTTCT TGTGAACACT
    TACTCGGGAG TCTGCAAAAT TTCCGATTTT GGAACATGCA AACGTCTTGC TGGGCTGAAT
    CCAGTAACCG AAACTTTCAC TGGAACTCTT CAGTATATGG CTCCAGAAGT TATCGATCAT
    GGACAACGTG GATATGGTGC ACCTGCAGAT ATTTGGTCAT TTGGGTGTAC AATGGTTGAA
    ATGGCAACTG GGCGGCCTCC GTTTGTTGAG ATGCAGAATC CGCAGGCGGC AATGTTCAGA
    GTTGGAATGT TCAAAACTCA TCCTCCAATT CCAACGGAAA TTACTGAAAA GTGTAGAAAC
    TTTATCAAAA GTTGTTTCTT GCCAGAAGCA TGTGATCGTC CATCAGCAAA AGATCTTCTC
    CAAGATCCAT TTATTTATCA CAACCATCAT TCTATTTCAA GAACGCGTAG CGGCTCGATC
    AACAAGAAAC CTGCCACCAA AATCGAGTTG AATCATGACA AGGAAAAGAA GGAAAAATCG
    AAAAATCAAC GAGAAATGCT CCGTTCAACA TCGCATATCG GTGGAATGGG AGTTGTTGAA
    AGATCTCCAC CAACACCTGA ACCAATGTCA GCCACTCTTA CTGCTGGATT CTCTCATGTT
    CACAGTCAAA CTGTATCGAA TGCATTGTCA ACTGCACGAG AAGAAAAGAA GCTTCATCTC
    AAGATTGATC ATGCAAGGAA TCGAACATTC AGTTCAAGTA GTCCAGTACC AGATGGTCAA
    TCATCTGCTG GAACTAATAT GTCTCATCCT GGATTCCAAT TGAGTCAGCC ATCAAGTCCT
    ATTGTCGATG ATACAAATCA CCCTCATTTG ATTGTCTCTC CAATTTCTCT GAATACAATG
    GGATCTCCAC TTTCATCAGC TGCACTTCTC AATCGAACAA TTTCCGATGA AAGTTCCAAT
    AGTTCAAGTC GATTCTTCAT GCTTCAAAAG GATTCAGAAC GTAGAAGATC CTTGGGACAA
    TTCATGCAAG ATTACAAAGA CTTGATCATT GACTCTTGGA GCACATTGCT CATTAAACAA
    AGTGACACGG AACTCGTAGT GACTGTCTAT ATGTTGGAAA TGTTACTTGA TGGAATGAGA
    GATTTCTTGT TGAAAAAGGA TAATACAAAG ATGCAAAAAA TGATTGATGA TATTCGAGGT
    CTTTTGGATT ATGATACTGC TAAAATTGGC CAGATAAACC TTGCTCTCTA CCACTTCTCC
    GATTCAATTC AACCTGTTCT CCGTCGCCTT GATATAAAGC CTCATTGGAT GTTTGCACTT
    TCAAATCTAA TCACTTCAGC CGTTCAATGT GCCATTTCAA TTCTAAGTCC AGATCTTTCT
    CTCCTACTTC ATGCTCAAGA TAATCTTCCA TCCACATCAT CAATTGTTGC AATCAGAAAT
    TCCTCACTTT CTGAAGGTGA AGCTCTCATT GAATCTCGAC CTCCAAGTCG TGAAGAACGA
    GTTCGTGAGG ATCGAAAAGA ATTGAGAACT TTACAAGAAG AAAATGAAAT TCTAATTGAA
    CGATTGCTTC AAGTGGAAAG AGAATTAAAT GCTCAACTCA AATCCGGAAT CACCAGAGCC
    AATCGTTTCC GAGATTTTGC CATGTACCGT AACACATATC CACCATTCCG TACTCCACCA
    GTCGCTCATG CTCCCCCAAC TCCTCCATTC AGTGCTTCAT GTGGTGCTCA ACCGTCAGGA
    ACATTTACAA ATCAACCTCC GAGCTTCGCC TCAATAAAAC CAATTGCTCA GAAAATCATA
    ATGCCACCCG GAACCGAGAA TAATTATCAA GTGACACGTG TTCAGGAGGA GCTTGTCAGT
    TGGCTACGTG GATTGGAAAT TGACGAACGA TCGATTGCTC TGATCGCCTC CGAAGCTTAT
    ACAAAGAGCG ATATGATGGA TTTTGTGACA AGAGACGAGC TGCTCAGTAT TGGTGTTGGC
    GGAGGTTCAT CTTGTCGTAT AATGCGTGCA ATTGGTGAAG TTCGTGAACG TCAACGCCGT
    CAGCCAGTCT TCCTGTCTCC AATGCGAAGT CGAGATGATT CTTTGGATGA TTATCATTCA
    AGCAGTGCCG ATGATATGTA CACTGGCGCT GCTGCTGAAA CTTCATCTGG AAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa339547
    Clone ID Related Accession (Same CDS sequence) NM_001313625.2 , NM_001313625.1
    Accession Version NM_001313625.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Protein kinase domain-containing protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001313625.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTAATG ATGTGTGTCC TTTGCCACTA CCGCCACGTG GCGCTCCGCC TCCAACCGCA 
    TACCACGCAA GTCGAATGGC TGCAACTTCA TCCTCAACCA ACGGATCTTT CGAAAAGTCA
    GCGATTCCAG GAAATGCACG AAAACTTCAC GTGGTGATTG TGATTGATCA GAAAGTGCAA
    AAGAATTTAC GCGTTCGAGA AATGGCTCTG AAAGATGTAC AGAAAGTGGC AGACACATTG
    AATGTTAATT TAACGCGCAT CGATTTTGAC AAATTGGACT TTGGCGAAAC AGAAACTCTG
    GATCTATTCT ACAATGCTGA CGTGGCACTT GTTGATGTTA CGGTAACCCA TCAACAACCA
    AGTCTTTGTT ATCATATTGG AGTTCGTGAG AGCATGGGAC AGTCATACAA TATGATACTC
    ACTTATTGGT CTCCTGATCC AGAATATCAT ATTATGGATG CTTTGAAGAA AACTCATGCA
    CATCTTCCAA TGATTGTATA TATTCATCAT CAAGATTCAA ATCAATTGCA GAGTTATGAT
    AAGAATAATA ACGATGATGA TTCAAAACCT CCATTTGCTA GAACAAATGT TCCAGCAAAA
    ACAATAACTT TTCAACATCG AATGAAACAA GTTTTGAAAA GTGTTCAAGT GGAAGCATCA
    GCACATTCTC GCGAGAAGTT CATGTCGGAT TTGCGAAAAG CTCGTGAAAT CACAGATGGA
    GATCAGAAGA ATGATTATCT TGATAAAATG AGAACAAGAC TTGATAATCC GGATGTATTA
    CATCCAGATA CCGTATCCCT TATGATGCTC TCTTATCGTG ATAATCAGAA TTATGGAGGA
    ATGATTCGTC TTGTTGATGA TTTGAAAAGA ATTCCGGATT GTTTGAAAGT TGTAGACACG
    CCGGTTATTC GATATCAGTA TGCGTTTGCT CTGAACCGAA GAAACAAGGA TGGGGACCGT
    GATTTGGCAT TAAATACCGT ACTCAGTTTG GTGGAGGGAA CAACTGAAAA CGAAGAGAAG
    AATGGCCCAT TGAGTCCAGA CGTTGTTTGT CTTGCTGGCC GTATCTACAA AGATAAATTC
    ATTGCATCCA ACTACGAAGA CCGTGAAAGC CTGAACAGTG CAATTGAATG GTATCGCCGT
    GCATTCGAAA TGTCACCTCT GGAATATTCA GGAATAAATC TGACAACTCT TCTTCGAGCA
    AGTGGAGAAC ACTTTGAGAA TAATCTCGAA ATGCAGCAAA TCGCTGTTGT CCTCAACTCG
    TTGCTCGGAC GGAAAGGGGC ACTTCAGAAC CTAATGGAAT ATTGGGATGT GGCAACTTAT
    TTCGAAGTTT CTGTTCTAGC AGAGAATTAC CAGAAAGCAT GTGAAGCAGC TTTGATGATG
    GTCAAGTTGA AACCTCCAGT TTGGTATTTA AAGTCTACGA TGGAAAATAT CAAATTAATA
    AATCGATGTG CAGCAACAAT CAGTCCAATC GAGAAGGAGA AGCAACAATT CCTTTTCTGG
    AGTGAATTCT TCATGGAGGC AACTGAGGCA GACACTGATA TTAGTTGCCC AAGATACCCA
    GTACTTATTC TGGAACTCAA TAAGGAATTC ACACCTTCTT ATCTGACTCT GAATAATGAA
    GAGGGAACAG TAATTCTGAG TCATGTTCTG GAAAACAGCC AACAGAAAAA GATTCATCAA
    TCCGAGTTGA GAGGTATACA TCGATGGCAT TTTGCAAGGA ATAATATCAA AGCAGTGACT
    GAATCGAAAC GAGATGATCG TCAATTATTC CTTTATGTTC ATGAAAATTC AGATGATTTC
    AACCTTCTGT TCCCGACAAA GGCTCATTGC AAAAAAGCCT ACGATGACAT GAAATCAATG
    GCCGATGTTG CTGATGGAAA CTACCAAGGA AGAGTACTAA GCAATCCGGA TAATGAGAAG
    ATCAGATTCG AGTATGAATT GAGTAATTCA AATGAGCGTG TCGTTCTTGG AAAAGGAACC
    TATGGAACTG TGTACGCTGC ACGAGATATG GATACTCAGC GACAAATTGT TGTAAAAGAA
    ATTGAAGTGA AGTATGATGA GGAAGTACAA CCGTTGATGG AAGAAATAAG TCTTCATTCC
    ACGTTGTGCC ATGCTAATAT TGTACAATAT CTTGGATGTG ATTTGGTTGG AAAAGATGGC
    TCAAATGATC ACTTTTTGAT TTTTATGGAG CATGTTCCTG GAGGATCACT CAGTTCATTG
    CTTCGTTCCA AGTGGGGTCC AATGAATGAA AATGCGATGA ACTACTACGG GAAGCAAATT
    CTGGAGGGTC TTAAGTATCT TCACGAGCTA AAAATTGTGC ATCGAGACAT CAAAGGAGAC
    AATGTTCTTG TGAACACTTA CTCGGGAGTC TGCAAAATTT CCGATTTTGG AACATGCAAA
    CGTCTTGCTG GGCTGAATCC AGTAACCGAA ACTTTCACTG GAACTCTTCA GTATATGGCT
    CCAGAAGTTA TCGATCATGG ACAACGTGGA TATGGTGCAC CTGCAGATAT TTGGTCATTT
    GGGTGTACAA TGGTTGAAAT GGCAACTGGG CGGCCTCCGT TTGTTGAGAT GCAGAATCCG
    CAGGCGGCAA TGTTCAGAGT TGGAATGTTC AAAACTCATC CTCCAATTCC AACGGAAATT
    ACTGAAAAGT GTAGAAACTT TATCAAAAGT TGTTTCTTGC CAGAAGCATG TGATCGTCCA
    TCAGCAAAAG ATCTTCTCCA AGATCCATTT ATTTATCACA ACCATCATTC AACGCGTAGC
    GGCTCGATCA ACAAGAAACC TGCCACCAAA ATCGAGTTGA ATCATGACAA GGAAAAGAAG
    GAAAAATCGA AAAATCAACG AGAAATGCTC CGTTCAACAT CGCATATCGG TGGAATGGGA
    GTTGTTGAAA GATCTCCACC AACACCTGAA CCAATGTCAG CCACTCTTAC TGCTGGATTC
    TCTCATGTTC ACAGTCAAAC TGTATCGAAT GCATTGTCAA CTGCACGAGA AGAAAAGAAG
    CTTCATCTCA AGATTGATCA TGCAAGGAAT CGAACATTCA GTTCAAGTAG TCCAGTACCA
    GATGGTCAAT CATCTGCTGG AACTAATATG TCTCATCCTG GATTCCAATT GAGTCAGCCA
    TCAAGTCCTA TTGTCGATGA TACAAATCAC CCTCATTTGA TTGTCTCTCC AATTTCTCTG
    AATACAATGG GATCTCCACT TTCATCAGCT GCACTTCTCA ATCGAACAAT TTCCGATGAA
    AGTTCCAATA GTTCAAGTCG ATTCTTCATG CTTCAAAAGG ATTCAGAACG TAGAAGATCC
    TTGGGACAAT TCATGCAAGA TTACAAAGAC TTGATCATTG ACTCTTGGAG CACATTGCTC
    ATTAAACAAA GTGACACGGA ACTCGTAGTG ACTGTCTATA TGTTGGAAAT GTTACTTGAT
    GGAATGAGAG ATTTCTTGTT GAAAAAGGAT AATACAAAGA TGCAAAAAAT GATTGATGAT
    ATTCGAGGTC TTTTGGATTA TGATACTGCT AAAATTGGCC AGATAAACCT TGCTCTCTAC
    CACTTCTCCG ATTCAATTCA ACCTGTTCTC CGTCGCCTTG ATATAAAGCC TCATTGGATG
    TTTGCACTTT CAAATCTAAT CACTTCAGCC GTTCAATGTG CCATTTCAAT TCTAAGTCCA
    GATCTTTCTC TCCTACTTCA TGCTCAAGAT AATCTTCCAT CCACATCATC AATTGTTGCA
    ATCAGAAATT CCTCACTTTC TGAAGGTGAA GCTCTCATTG AATCTCGACC TCCAAGTCGT
    GAAGAACGAG TTCGTGAGGA TCGAAAAGAA TTGAGAACTT TACAAGAAGA AAATGAAATT
    CTAATTGAAC GATTGCTTCA AGTGGAAAGA GAATTAAATG CTCAACTCAA ATCCGGAATC
    ACCAGAGCCA ATCGTTTCCG AGATTTTGCC ATGTACCGTA ACACATATCC ACCATTCCGT
    ACTCCACCAG TCGCTCATGC TCCCCCAACT CCTCCATTCA GTGCTTCATG TGGTGCTCAA
    CCGTCAGGAA CATTTACAAA TCAACCTCCG AGCTTCGCCT CAATAAAACC AATTGCTCAG
    AAAATCATAA TGCCACCCGG AACCGAGAAT AATTATCAAG TGACACGTGT TCAGGAGGAG
    CTTGTCAGTT GGCTACGTGG ATTGGAAATT GACGAACGAT CGATTGCTCT GATCGCCTCC
    GAAGCTTATA CAAAGAGCGA TATGATGGAT TTTGTGACAA GAGACGAGCT GCTCAGTATT
    GGTGTTGGCG GAGGTTCATC TTGTCGTATA ATGCGTGCAA TTGGTGAAGT TCGTGAACGT
    CAACGCCGTC AGCCAGTCTT CCTGTCTCCA ATGCGAAGTC GAGATGATTC TTTGGATGAT
    TATCATTCAA GCAGTGCCGA TGATATGTAC ACTGGCGCTG CTGCTGAAAC TTCATCTGGA
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001300554.1
    CDS1..4446
    Translation

    Target ORF information:

    RefSeq Version NM_001313625.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Protein kinase domain-containing protein (nsy-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001313625.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTAATG ATGTGTGTCC TTTGCCACTA CCGCCACGTG GCGCTCCGCC TCCAACCGCA 
    TACCACGCAA GTCGAATGGC TGCAACTTCA TCCTCAACCA ACGGATCTTT CGAAAAGTCA
    GCGATTCCAG GAAATGCACG AAAACTTCAC GTGGTGATTG TGATTGATCA GAAAGTGCAA
    AAGAATTTAC GCGTTCGAGA AATGGCTCTG AAAGATGTAC AGAAAGTGGC AGACACATTG
    AATGTTAATT TAACGCGCAT CGATTTTGAC AAATTGGACT TTGGCGAAAC AGAAACTCTG
    GATCTATTCT ACAATGCTGA CGTGGCACTT GTTGATGTTA CGGTAACCCA TCAACAACCA
    AGTCTTTGTT ATCATATTGG AGTTCGTGAG AGCATGGGAC AGTCATACAA TATGATACTC
    ACTTATTGGT CTCCTGATCC AGAATATCAT ATTATGGATG CTTTGAAGAA AACTCATGCA
    CATCTTCCAA TGATTGTATA TATTCATCAT CAAGATTCAA ATCAATTGCA GAGTTATGAT
    AAGAATAATA ACGATGATGA TTCAAAACCT CCATTTGCTA GAACAAATGT TCCAGCAAAA
    ACAATAACTT TTCAACATCG AATGAAACAA GTTTTGAAAA GTGTTCAAGT GGAAGCATCA
    GCACATTCTC GCGAGAAGTT CATGTCGGAT TTGCGAAAAG CTCGTGAAAT CACAGATGGA
    GATCAGAAGA ATGATTATCT TGATAAAATG AGAACAAGAC TTGATAATCC GGATGTATTA
    CATCCAGATA CCGTATCCCT TATGATGCTC TCTTATCGTG ATAATCAGAA TTATGGAGGA
    ATGATTCGTC TTGTTGATGA TTTGAAAAGA ATTCCGGATT GTTTGAAAGT TGTAGACACG
    CCGGTTATTC GATATCAGTA TGCGTTTGCT CTGAACCGAA GAAACAAGGA TGGGGACCGT
    GATTTGGCAT TAAATACCGT ACTCAGTTTG GTGGAGGGAA CAACTGAAAA CGAAGAGAAG
    AATGGCCCAT TGAGTCCAGA CGTTGTTTGT CTTGCTGGCC GTATCTACAA AGATAAATTC
    ATTGCATCCA ACTACGAAGA CCGTGAAAGC CTGAACAGTG CAATTGAATG GTATCGCCGT
    GCATTCGAAA TGTCACCTCT GGAATATTCA GGAATAAATC TGACAACTCT TCTTCGAGCA
    AGTGGAGAAC ACTTTGAGAA TAATCTCGAA ATGCAGCAAA TCGCTGTTGT CCTCAACTCG
    TTGCTCGGAC GGAAAGGGGC ACTTCAGAAC CTAATGGAAT ATTGGGATGT GGCAACTTAT
    TTCGAAGTTT CTGTTCTAGC AGAGAATTAC CAGAAAGCAT GTGAAGCAGC TTTGATGATG
    GTCAAGTTGA AACCTCCAGT TTGGTATTTA AAGTCTACGA TGGAAAATAT CAAATTAATA
    AATCGATGTG CAGCAACAAT CAGTCCAATC GAGAAGGAGA AGCAACAATT CCTTTTCTGG
    AGTGAATTCT TCATGGAGGC AACTGAGGCA GACACTGATA TTAGTTGCCC AAGATACCCA
    GTACTTATTC TGGAACTCAA TAAGGAATTC ACACCTTCTT ATCTGACTCT GAATAATGAA
    GAGGGAACAG TAATTCTGAG TCATGTTCTG GAAAACAGCC AACAGAAAAA GATTCATCAA
    TCCGAGTTGA GAGGTATACA TCGATGGCAT TTTGCAAGGA ATAATATCAA AGCAGTGACT
    GAATCGAAAC GAGATGATCG TCAATTATTC CTTTATGTTC ATGAAAATTC AGATGATTTC
    AACCTTCTGT TCCCGACAAA GGCTCATTGC AAAAAAGCCT ACGATGACAT GAAATCAATG
    GCCGATGTTG CTGATGGAAA CTACCAAGGA AGAGTACTAA GCAATCCGGA TAATGAGAAG
    ATCAGATTCG AGTATGAATT GAGTAATTCA AATGAGCGTG TCGTTCTTGG AAAAGGAACC
    TATGGAACTG TGTACGCTGC ACGAGATATG GATACTCAGC GACAAATTGT TGTAAAAGAA
    ATTGAAGTGA AGTATGATGA GGAAGTACAA CCGTTGATGG AAGAAATAAG TCTTCATTCC
    ACGTTGTGCC ATGCTAATAT TGTACAATAT CTTGGATGTG ATTTGGTTGG AAAAGATGGC
    TCAAATGATC ACTTTTTGAT TTTTATGGAG CATGTTCCTG GAGGATCACT CAGTTCATTG
    CTTCGTTCCA AGTGGGGTCC AATGAATGAA AATGCGATGA ACTACTACGG GAAGCAAATT
    CTGGAGGGTC TTAAGTATCT TCACGAGCTA AAAATTGTGC ATCGAGACAT CAAAGGAGAC
    AATGTTCTTG TGAACACTTA CTCGGGAGTC TGCAAAATTT CCGATTTTGG AACATGCAAA
    CGTCTTGCTG GGCTGAATCC AGTAACCGAA ACTTTCACTG GAACTCTTCA GTATATGGCT
    CCAGAAGTTA TCGATCATGG ACAACGTGGA TATGGTGCAC CTGCAGATAT TTGGTCATTT
    GGGTGTACAA TGGTTGAAAT GGCAACTGGG CGGCCTCCGT TTGTTGAGAT GCAGAATCCG
    CAGGCGGCAA TGTTCAGAGT TGGAATGTTC AAAACTCATC CTCCAATTCC AACGGAAATT
    ACTGAAAAGT GTAGAAACTT TATCAAAAGT TGTTTCTTGC CAGAAGCATG TGATCGTCCA
    TCAGCAAAAG ATCTTCTCCA AGATCCATTT ATTTATCACA ACCATCATTC AACGCGTAGC
    GGCTCGATCA ACAAGAAACC TGCCACCAAA ATCGAGTTGA ATCATGACAA GGAAAAGAAG
    GAAAAATCGA AAAATCAACG AGAAATGCTC CGTTCAACAT CGCATATCGG TGGAATGGGA
    GTTGTTGAAA GATCTCCACC AACACCTGAA CCAATGTCAG CCACTCTTAC TGCTGGATTC
    TCTCATGTTC ACAGTCAAAC TGTATCGAAT GCATTGTCAA CTGCACGAGA AGAAAAGAAG
    CTTCATCTCA AGATTGATCA TGCAAGGAAT CGAACATTCA GTTCAAGTAG TCCAGTACCA
    GATGGTCAAT CATCTGCTGG AACTAATATG TCTCATCCTG GATTCCAATT GAGTCAGCCA
    TCAAGTCCTA TTGTCGATGA TACAAATCAC CCTCATTTGA TTGTCTCTCC AATTTCTCTG
    AATACAATGG GATCTCCACT TTCATCAGCT GCACTTCTCA ATCGAACAAT TTCCGATGAA
    AGTTCCAATA GTTCAAGTCG ATTCTTCATG CTTCAAAAGG ATTCAGAACG TAGAAGATCC
    TTGGGACAAT TCATGCAAGA TTACAAAGAC TTGATCATTG ACTCTTGGAG CACATTGCTC
    ATTAAACAAA GTGACACGGA ACTCGTAGTG ACTGTCTATA TGTTGGAAAT GTTACTTGAT
    GGAATGAGAG ATTTCTTGTT GAAAAAGGAT AATACAAAGA TGCAAAAAAT GATTGATGAT
    ATTCGAGGTC TTTTGGATTA TGATACTGCT AAAATTGGCC AGATAAACCT TGCTCTCTAC
    CACTTCTCCG ATTCAATTCA ACCTGTTCTC CGTCGCCTTG ATATAAAGCC TCATTGGATG
    TTTGCACTTT CAAATCTAAT CACTTCAGCC GTTCAATGTG CCATTTCAAT TCTAAGTCCA
    GATCTTTCTC TCCTACTTCA TGCTCAAGAT AATCTTCCAT CCACATCATC AATTGTTGCA
    ATCAGAAATT CCTCACTTTC TGAAGGTGAA GCTCTCATTG AATCTCGACC TCCAAGTCGT
    GAAGAACGAG TTCGTGAGGA TCGAAAAGAA TTGAGAACTT TACAAGAAGA AAATGAAATT
    CTAATTGAAC GATTGCTTCA AGTGGAAAGA GAATTAAATG CTCAACTCAA ATCCGGAATC
    ACCAGAGCCA ATCGTTTCCG AGATTTTGCC ATGTACCGTA ACACATATCC ACCATTCCGT
    ACTCCACCAG TCGCTCATGC TCCCCCAACT CCTCCATTCA GTGCTTCATG TGGTGCTCAA
    CCGTCAGGAA CATTTACAAA TCAACCTCCG AGCTTCGCCT CAATAAAACC AATTGCTCAG
    AAAATCATAA TGCCACCCGG AACCGAGAAT AATTATCAAG TGACACGTGT TCAGGAGGAG
    CTTGTCAGTT GGCTACGTGG ATTGGAAATT GACGAACGAT CGATTGCTCT GATCGCCTCC
    GAAGCTTATA CAAAGAGCGA TATGATGGAT TTTGTGACAA GAGACGAGCT GCTCAGTATT
    GGTGTTGGCG GAGGTTCATC TTGTCGTATA ATGCGTGCAA TTGGTGAAGT TCGTGAACGT
    CAACGCCGTC AGCCAGTCTT CCTGTCTCCA ATGCGAAGTC GAGATGATTC TTTGGATGAT
    TATCATTCAA GCAGTGCCGA TGATATGTAC ACTGGCGCTG CTGCTGAAAC TTCATCTGGA
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa339547
    Clone ID Related Accession (Same CDS sequence) NM_001313625.2 , NM_001313625.1
    Accession Version NM_001313625.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Mitogen-activated protein kinase kinase kinase nsy-1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTAATG ATGTGTGTCC TTTGCCACTA CCGCCACGTG GCGCTCCGCC TCCAACCGCA 
    TACCACGCAA GTCGAATGGC TGCAACTTCA TCCTCAACCA ACGGATCTTT CGAAAAGTCA
    GCGATTCCAG GAAATGCACG AAAACTTCAC GTGGTGATTG TGATTGATCA GAAAGTGCAA
    AAGAATTTAC GCGTTCGAGA AATGGCTCTG AAAGATGTAC AGAAAGTGGC AGACACATTG
    AATGTTAATT TAACGCGCAT CGATTTTGAC AAATTGGACT TTGGCGAAAC AGAAACTCTG
    GATCTATTCT ACAATGCTGA CGTGGCACTT GTTGATGTTA CGGTAACCCA TCAACAACCA
    AGTCTTTGTT ATCATATTGG AGTTCGTGAG AGCATGGGAC AGTCATACAA TATGATACTC
    ACTTATTGGT CTCCTGATCC AGAATATCAT ATTATGGATG CTTTGAAGAA AACTCATGCA
    CATCTTCCAA TGATTGTATA TATTCATCAT CAAGATTCAA ATCAATTGCA GAGTTATGAT
    AAGAATAATA ACGATGATGA TTCAAAACCT CCATTTGCTA GAACAAATGT TCCAGCAAAA
    ACAATAACTT TTCAACATCG AATGAAACAA GTTTTGAAAA GTGTTCAAGT GGAAGCATCA
    GCACATTCTC GCGAGAAGTT CATGTCGGAT TTGCGAAAAG CTCGTGAAAT CACAGATGGA
    GATCAGAAGA ATGATTATCT TGATAAAATG AGAACAAGAC TTGATAATCC GGATGTATTA
    CATCCAGATA CCGTATCCCT TATGATGCTC TCTTATCGTG ATAATCAGAA TTATGGAGGA
    ATGATTCGTC TTGTTGATGA TTTGAAAAGA ATTCCGGATT GTTTGAAAGT TGTAGACACG
    CCGGTTATTC GATATCAGTA TGCGTTTGCT CTGAACCGAA GAAACAAGGA TGGGGACCGT
    GATTTGGCAT TAAATACCGT ACTCAGTTTG GTGGAGGGAA CAACTGAAAA CGAAGAGAAG
    AATGGCCCAT TGAGTCCAGA CGTTGTTTGT CTTGCTGGCC GTATCTACAA AGATAAATTC
    ATTGCATCCA ACTACGAAGA CCGTGAAAGC CTGAACAGTG CAATTGAATG GTATCGCCGT
    GCATTCGAAA TGTCACCTCT GGAATATTCA GGAATAAATC TGACAACTCT TCTTCGAGCA
    AGTGGAGAAC ACTTTGAGAA TAATCTCGAA ATGCAGCAAA TCGCTGTTGT CCTCAACTCG
    TTGCTCGGAC GGAAAGGGGC ACTTCAGAAC CTAATGGAAT ATTGGGATGT GGCAACTTAT
    TTCGAAGTTT CTGTTCTAGC AGAGAATTAC CAGAAAGCAT GTGAAGCAGC TTTGATGATG
    GTCAAGTTGA AACCTCCAGT TTGGTATTTA AAGTCTACGA TGGAAAATAT CAAATTAATA
    AATCGATGTG CAGCAACAAT CAGTCCAATC GAGAAGGAGA AGCAACAATT CCTTTTCTGG
    AGTGAATTCT TCATGGAGGC AACTGAGGCA GACACTGATA TTAGTTGCCC AAGATACCCA
    GTACTTATTC TGGAACTCAA TAAGGAATTC ACACCTTCTT ATCTGACTCT GAATAATGAA
    GAGGGAACAG TAATTCTGAG TCATGTTCTG GAAAACAGCC AACAGAAAAA GATTCATCAA
    TCCGAGTTGA GAGGTATACA TCGATGGCAT TTTGCAAGGA ATAATATCAA AGCAGTGACT
    GAATCGAAAC GAGATGATCG TCAATTATTC CTTTATGTTC ATGAAAATTC AGATGATTTC
    AACCTTCTGT TCCCGACAAA GGCTCATTGC AAAAAAGCCT ACGATGACAT GAAATCAATG
    GCCGATGTTG CTGATGGAAA CTACCAAGGA AGAGTACTAA GCAATCCGGA TAATGAGAAG
    ATCAGATTCG AGTATGAATT GAGTAATTCA AATGAGCGTG TCGTTCTTGG AAAAGGAACC
    TATGGAACTG TGTACGCTGC ACGAGATATG GATACTCAGC GACAAATTGT TGTAAAAGAA
    ATTGAAGTGA AGTATGATGA GGAAGTACAA CCGTTGATGG AAGAAATAAG TCTTCATTCC
    ACGTTGTGCC ATGCTAATAT TGTACAATAT CTTGGATGTG ATTTGGTTGG AAAAGATGGC
    TCAAATGATC ACTTTTTGAT TTTTATGGAG CATGTTCCTG GAGGATCACT CAGTTCATTG
    CTTCGTTCCA AGTGGGGTCC AATGAATGAA AATGCGATGA ACTACTACGG GAAGCAAATT
    CTGGAGGGTC TTAAGTATCT TCACGAGCTA AAAATTGTGC ATCGAGACAT CAAAGGAGAC
    AATGTTCTTG TGAACACTTA CTCGGGAGTC TGCAAAATTT CCGATTTTGG AACATGCAAA
    CGTCTTGCTG GGCTGAATCC AGTAACCGAA ACTTTCACTG GAACTCTTCA GTATATGGCT
    CCAGAAGTTA TCGATCATGG ACAACGTGGA TATGGTGCAC CTGCAGATAT TTGGTCATTT
    GGGTGTACAA TGGTTGAAAT GGCAACTGGG CGGCCTCCGT TTGTTGAGAT GCAGAATCCG
    CAGGCGGCAA TGTTCAGAGT TGGAATGTTC AAAACTCATC CTCCAATTCC AACGGAAATT
    ACTGAAAAGT GTAGAAACTT TATCAAAAGT TGTTTCTTGC CAGAAGCATG TGATCGTCCA
    TCAGCAAAAG ATCTTCTCCA AGATCCATTT ATTTATCACA ACCATCATTC AACGCGTAGC
    GGCTCGATCA ACAAGAAACC TGCCACCAAA ATCGAGTTGA ATCATGACAA GGAAAAGAAG
    GAAAAATCGA AAAATCAACG AGAAATGCTC CGTTCAACAT CGCATATCGG TGGAATGGGA
    GTTGTTGAAA GATCTCCACC AACACCTGAA CCAATGTCAG CCACTCTTAC TGCTGGATTC
    TCTCATGTTC ACAGTCAAAC TGTATCGAAT GCATTGTCAA CTGCACGAGA AGAAAAGAAG
    CTTCATCTCA AGATTGATCA TGCAAGGAAT CGAACATTCA GTTCAAGTAG TCCAGTACCA
    GATGGTCAAT CATCTGCTGG AACTAATATG TCTCATCCTG GATTCCAATT GAGTCAGCCA
    TCAAGTCCTA TTGTCGATGA TACAAATCAC CCTCATTTGA TTGTCTCTCC AATTTCTCTG
    AATACAATGG GATCTCCACT TTCATCAGCT GCACTTCTCA ATCGAACAAT TTCCGATGAA
    AGTTCCAATA GTTCAAGTCG ATTCTTCATG CTTCAAAAGG ATTCAGAACG TAGAAGATCC
    TTGGGACAAT TCATGCAAGA TTACAAAGAC TTGATCATTG ACTCTTGGAG CACATTGCTC
    ATTAAACAAA GTGACACGGA ACTCGTAGTG ACTGTCTATA TGTTGGAAAT GTTACTTGAT
    GGAATGAGAG ATTTCTTGTT GAAAAAGGAT AATACAAAGA TGCAAAAAAT GATTGATGAT
    ATTCGAGGTC TTTTGGATTA TGATACTGCT AAAATTGGCC AGATAAACCT TGCTCTCTAC
    CACTTCTCCG ATTCAATTCA ACCTGTTCTC CGTCGCCTTG ATATAAAGCC TCATTGGATG
    TTTGCACTTT CAAATCTAAT CACTTCAGCC GTTCAATGTG CCATTTCAAT TCTAAGTCCA
    GATCTTTCTC TCCTACTTCA TGCTCAAGAT AATCTTCCAT CCACATCATC AATTGTTGCA
    ATCAGAAATT CCTCACTTTC TGAAGGTGAA GCTCTCATTG AATCTCGACC TCCAAGTCGT
    GAAGAACGAG TTCGTGAGGA TCGAAAAGAA TTGAGAACTT TACAAGAAGA AAATGAAATT
    CTAATTGAAC GATTGCTTCA AGTGGAAAGA GAATTAAATG CTCAACTCAA ATCCGGAATC
    ACCAGAGCCA ATCGTTTCCG AGATTTTGCC ATGTACCGTA ACACATATCC ACCATTCCGT
    ACTCCACCAG TCGCTCATGC TCCCCCAACT CCTCCATTCA GTGCTTCATG TGGTGCTCAA
    CCGTCAGGAA CATTTACAAA TCAACCTCCG AGCTTCGCCT CAATAAAACC AATTGCTCAG
    AAAATCATAA TGCCACCCGG AACCGAGAAT AATTATCAAG TGACACGTGT TCAGGAGGAG
    CTTGTCAGTT GGCTACGTGG ATTGGAAATT GACGAACGAT CGATTGCTCT GATCGCCTCC
    GAAGCTTATA CAAAGAGCGA TATGATGGAT TTTGTGACAA GAGACGAGCT GCTCAGTATT
    GGTGTTGGCG GAGGTTCATC TTGTCGTATA ATGCGTGCAA TTGGTGAAGT TCGTGAACGT
    CAACGCCGTC AGCCAGTCTT CCTGTCTCCA ATGCGAAGTC GAGATGATTC TTTGGATGAT
    TATCATTCAA GCAGTGCCGA TGATATGTAC ACTGGCGCTG CTGCTGAAAC TTCATCTGGA
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001300554.1
    CDS1..4446
    Translation

    Target ORF information:

    RefSeq Version NM_001313625.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Mitogen-activated protein kinase kinase kinase nsy-1 (nsy-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001313625.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTAATG ATGTGTGTCC TTTGCCACTA CCGCCACGTG GCGCTCCGCC TCCAACCGCA 
    TACCACGCAA GTCGAATGGC TGCAACTTCA TCCTCAACCA ACGGATCTTT CGAAAAGTCA
    GCGATTCCAG GAAATGCACG AAAACTTCAC GTGGTGATTG TGATTGATCA GAAAGTGCAA
    AAGAATTTAC GCGTTCGAGA AATGGCTCTG AAAGATGTAC AGAAAGTGGC AGACACATTG
    AATGTTAATT TAACGCGCAT CGATTTTGAC AAATTGGACT TTGGCGAAAC AGAAACTCTG
    GATCTATTCT ACAATGCTGA CGTGGCACTT GTTGATGTTA CGGTAACCCA TCAACAACCA
    AGTCTTTGTT ATCATATTGG AGTTCGTGAG AGCATGGGAC AGTCATACAA TATGATACTC
    ACTTATTGGT CTCCTGATCC AGAATATCAT ATTATGGATG CTTTGAAGAA AACTCATGCA
    CATCTTCCAA TGATTGTATA TATTCATCAT CAAGATTCAA ATCAATTGCA GAGTTATGAT
    AAGAATAATA ACGATGATGA TTCAAAACCT CCATTTGCTA GAACAAATGT TCCAGCAAAA
    ACAATAACTT TTCAACATCG AATGAAACAA GTTTTGAAAA GTGTTCAAGT GGAAGCATCA
    GCACATTCTC GCGAGAAGTT CATGTCGGAT TTGCGAAAAG CTCGTGAAAT CACAGATGGA
    GATCAGAAGA ATGATTATCT TGATAAAATG AGAACAAGAC TTGATAATCC GGATGTATTA
    CATCCAGATA CCGTATCCCT TATGATGCTC TCTTATCGTG ATAATCAGAA TTATGGAGGA
    ATGATTCGTC TTGTTGATGA TTTGAAAAGA ATTCCGGATT GTTTGAAAGT TGTAGACACG
    CCGGTTATTC GATATCAGTA TGCGTTTGCT CTGAACCGAA GAAACAAGGA TGGGGACCGT
    GATTTGGCAT TAAATACCGT ACTCAGTTTG GTGGAGGGAA CAACTGAAAA CGAAGAGAAG
    AATGGCCCAT TGAGTCCAGA CGTTGTTTGT CTTGCTGGCC GTATCTACAA AGATAAATTC
    ATTGCATCCA ACTACGAAGA CCGTGAAAGC CTGAACAGTG CAATTGAATG GTATCGCCGT
    GCATTCGAAA TGTCACCTCT GGAATATTCA GGAATAAATC TGACAACTCT TCTTCGAGCA
    AGTGGAGAAC ACTTTGAGAA TAATCTCGAA ATGCAGCAAA TCGCTGTTGT CCTCAACTCG
    TTGCTCGGAC GGAAAGGGGC ACTTCAGAAC CTAATGGAAT ATTGGGATGT GGCAACTTAT
    TTCGAAGTTT CTGTTCTAGC AGAGAATTAC CAGAAAGCAT GTGAAGCAGC TTTGATGATG
    GTCAAGTTGA AACCTCCAGT TTGGTATTTA AAGTCTACGA TGGAAAATAT CAAATTAATA
    AATCGATGTG CAGCAACAAT CAGTCCAATC GAGAAGGAGA AGCAACAATT CCTTTTCTGG
    AGTGAATTCT TCATGGAGGC AACTGAGGCA GACACTGATA TTAGTTGCCC AAGATACCCA
    GTACTTATTC TGGAACTCAA TAAGGAATTC ACACCTTCTT ATCTGACTCT GAATAATGAA
    GAGGGAACAG TAATTCTGAG TCATGTTCTG GAAAACAGCC AACAGAAAAA GATTCATCAA
    TCCGAGTTGA GAGGTATACA TCGATGGCAT TTTGCAAGGA ATAATATCAA AGCAGTGACT
    GAATCGAAAC GAGATGATCG TCAATTATTC CTTTATGTTC ATGAAAATTC AGATGATTTC
    AACCTTCTGT TCCCGACAAA GGCTCATTGC AAAAAAGCCT ACGATGACAT GAAATCAATG
    GCCGATGTTG CTGATGGAAA CTACCAAGGA AGAGTACTAA GCAATCCGGA TAATGAGAAG
    ATCAGATTCG AGTATGAATT GAGTAATTCA AATGAGCGTG TCGTTCTTGG AAAAGGAACC
    TATGGAACTG TGTACGCTGC ACGAGATATG GATACTCAGC GACAAATTGT TGTAAAAGAA
    ATTGAAGTGA AGTATGATGA GGAAGTACAA CCGTTGATGG AAGAAATAAG TCTTCATTCC
    ACGTTGTGCC ATGCTAATAT TGTACAATAT CTTGGATGTG ATTTGGTTGG AAAAGATGGC
    TCAAATGATC ACTTTTTGAT TTTTATGGAG CATGTTCCTG GAGGATCACT CAGTTCATTG
    CTTCGTTCCA AGTGGGGTCC AATGAATGAA AATGCGATGA ACTACTACGG GAAGCAAATT
    CTGGAGGGTC TTAAGTATCT TCACGAGCTA AAAATTGTGC ATCGAGACAT CAAAGGAGAC
    AATGTTCTTG TGAACACTTA CTCGGGAGTC TGCAAAATTT CCGATTTTGG AACATGCAAA
    CGTCTTGCTG GGCTGAATCC AGTAACCGAA ACTTTCACTG GAACTCTTCA GTATATGGCT
    CCAGAAGTTA TCGATCATGG ACAACGTGGA TATGGTGCAC CTGCAGATAT TTGGTCATTT
    GGGTGTACAA TGGTTGAAAT GGCAACTGGG CGGCCTCCGT TTGTTGAGAT GCAGAATCCG
    CAGGCGGCAA TGTTCAGAGT TGGAATGTTC AAAACTCATC CTCCAATTCC AACGGAAATT
    ACTGAAAAGT GTAGAAACTT TATCAAAAGT TGTTTCTTGC CAGAAGCATG TGATCGTCCA
    TCAGCAAAAG ATCTTCTCCA AGATCCATTT ATTTATCACA ACCATCATTC AACGCGTAGC
    GGCTCGATCA ACAAGAAACC TGCCACCAAA ATCGAGTTGA ATCATGACAA GGAAAAGAAG
    GAAAAATCGA AAAATCAACG AGAAATGCTC CGTTCAACAT CGCATATCGG TGGAATGGGA
    GTTGTTGAAA GATCTCCACC AACACCTGAA CCAATGTCAG CCACTCTTAC TGCTGGATTC
    TCTCATGTTC ACAGTCAAAC TGTATCGAAT GCATTGTCAA CTGCACGAGA AGAAAAGAAG
    CTTCATCTCA AGATTGATCA TGCAAGGAAT CGAACATTCA GTTCAAGTAG TCCAGTACCA
    GATGGTCAAT CATCTGCTGG AACTAATATG TCTCATCCTG GATTCCAATT GAGTCAGCCA
    TCAAGTCCTA TTGTCGATGA TACAAATCAC CCTCATTTGA TTGTCTCTCC AATTTCTCTG
    AATACAATGG GATCTCCACT TTCATCAGCT GCACTTCTCA ATCGAACAAT TTCCGATGAA
    AGTTCCAATA GTTCAAGTCG ATTCTTCATG CTTCAAAAGG ATTCAGAACG TAGAAGATCC
    TTGGGACAAT TCATGCAAGA TTACAAAGAC TTGATCATTG ACTCTTGGAG CACATTGCTC
    ATTAAACAAA GTGACACGGA ACTCGTAGTG ACTGTCTATA TGTTGGAAAT GTTACTTGAT
    GGAATGAGAG ATTTCTTGTT GAAAAAGGAT AATACAAAGA TGCAAAAAAT GATTGATGAT
    ATTCGAGGTC TTTTGGATTA TGATACTGCT AAAATTGGCC AGATAAACCT TGCTCTCTAC
    CACTTCTCCG ATTCAATTCA ACCTGTTCTC CGTCGCCTTG ATATAAAGCC TCATTGGATG
    TTTGCACTTT CAAATCTAAT CACTTCAGCC GTTCAATGTG CCATTTCAAT TCTAAGTCCA
    GATCTTTCTC TCCTACTTCA TGCTCAAGAT AATCTTCCAT CCACATCATC AATTGTTGCA
    ATCAGAAATT CCTCACTTTC TGAAGGTGAA GCTCTCATTG AATCTCGACC TCCAAGTCGT
    GAAGAACGAG TTCGTGAGGA TCGAAAAGAA TTGAGAACTT TACAAGAAGA AAATGAAATT
    CTAATTGAAC GATTGCTTCA AGTGGAAAGA GAATTAAATG CTCAACTCAA ATCCGGAATC
    ACCAGAGCCA ATCGTTTCCG AGATTTTGCC ATGTACCGTA ACACATATCC ACCATTCCGT
    ACTCCACCAG TCGCTCATGC TCCCCCAACT CCTCCATTCA GTGCTTCATG TGGTGCTCAA
    CCGTCAGGAA CATTTACAAA TCAACCTCCG AGCTTCGCCT CAATAAAACC AATTGCTCAG
    AAAATCATAA TGCCACCCGG AACCGAGAAT AATTATCAAG TGACACGTGT TCAGGAGGAG
    CTTGTCAGTT GGCTACGTGG ATTGGAAATT GACGAACGAT CGATTGCTCT GATCGCCTCC
    GAAGCTTATA CAAAGAGCGA TATGATGGAT TTTGTGACAA GAGACGAGCT GCTCAGTATT
    GGTGTTGGCG GAGGTTCATC TTGTCGTATA ATGCGTGCAA TTGGTGAAGT TCGTGAACGT
    CAACGCCGTC AGCCAGTCTT CCTGTCTCCA ATGCGAAGTC GAGATGATTC TTTGGATGAT
    TATCATTCAA GCAGTGCCGA TGATATGTAC ACTGGCGCTG CTGCTGAAAC TTCATCTGGA
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.