Y54E2A.4 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	Y54E2A.4
Entrez Gene ID	190256
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001306606.2	NP_001293535.1	SEC63 domain-containing protein
NM_064659.2	NP_497060.2	hypothetical protein
NM_001306606.1	NP_001293535.1	hypothetical protein
NM_001306607.1	NP_001293536.1	hypothetical protein
NM_064659.3	NP_497060.2	SEC63 domain-containing protein
NM_001306607.2	NP_001293536.1	SEC63 domain-containing protein

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ascc3	XP_002664072.3
Magnaporthe oryzae (rice blast fungus)	MGG_06124	XP_003711991.1
Drosophila melanogaster (fruit fly)	CG5205	NP_650472.2
Saccharomyces cerevisiae (baker's yeast)	SLH1	NP_011787.4
Neurospora crassa	NCU08267	XP_961895.1
Oryza sativa (rice)	Os03g0213400	NP_001049362.1
Bos taurus (cattle)	ASCC3	NP_001193047.1
Mus musculus (house mouse)	Ascc3	NP_932124.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001234	XP_321922.5
Arabidopsis thaliana (thale cress)	AT5G61140	NP_001190584.1
Canis lupus familiaris (dog)	ASCC3	XP_854167.1
Gallus gallus (chicken)	ASCC3	XP_419816.1
Kluyveromyces lactis	KLLA0A09889g	XP_451432.1
Eremothecium gossypii	AGOS_ABR142W	NP_983089.2
Schizosaccharomyces pombe (fission yeast)	mug81	NP_596560.1
Homo sapiens (human)	ASCC3	NP_006819.2
Pan troglodytes (chimpanzee)	ASCC3	XP_518652.3
	ASCC3	XP_002803889.1
Rattus norvegicus (Norway rat)	Ascc3	NP_001263986.1
Caenorhabditis elegans (roundworm)	CELE_Y54E2A.4	NP_497060.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Y54E2A.4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Y54E2A.4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa360032	NM_001306607.2 Latest version!	Caenorhabditis elegans SEC63 domain-containing protein (Y54E2A.4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OCa360031	NM_001306606.2 Latest version!	Caenorhabditis elegans SEC63 domain-containing protein (Y54E2A.4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa360030	NM_064659.3 Latest version!	Caenorhabditis elegans SEC63 domain-containing protein (Y54E2A.4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCa360031	NM_001306606.1	Caenorhabditis elegans Uncharacterized protein (Y54E2A.4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCa360030	NM_064659.2	Caenorhabditis elegans Uncharacterized protein (Y54E2A.4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa360032	NM_001306607.1	Caenorhabditis elegans Uncharacterized protein (Y54E2A.4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Y54E2A.4 ( NM_001306607.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293536.1 Caenorhabditis elegans Uncharacterized protein (Y54E2A.4), partial mRNA.

Price & Availability↑

CloneID	OCa360032
Clone ID Related Accession (Same CDS sequence)	NM_001306607.1 , NM_001306607.2
Accession Version	NM_001306607.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-09
Organism	Caenorhabditis elegans
Product	hypothetical protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGATGGGCC GCGCCGGACG CCCACAATTC GACGATTCGG CCGTTGCCGT GATCTACGTA 
CAGGACGCGA AAAAGACGTT CTATAAGAAG TTCCTCTACG AACCGTTCCC CGTCGAGTCC 
TCGCTCCTTC CAGTGCTGCC GAATCACGTG AACGCCGAGA TTTCCGCCGG AACAATCGAT 
TCGAAGCAGG CGATCGTCGA GTATTTGTCG AAAACCTATC TCTACAGGCG ATTGTTTGCG 
AATCCCAATT ATTACGGTCT GGAGGAGGAT TCCGAGGAGG CGATGCTCAA GTTTATCACG 
AAAATCGTTG ATGACTCTGT CGCAGAGCTC CTAGCTTCCG AGTGTATCCA CGTGGATTCC 
GAACAGGACG TGATCAAGCC GACGCCGTGT GGAAGGATAG CTTCGGTGTA CTATCTTCAG 
CACGAGACCG TCCGTTTTCT AGTAAAATCT CTACATTCTG GATGCTCGGT GGAGAATATG 
CTGAAGATCC TGACCGATGT TCCCGAGTAC GCGGAGATTC CTGTCCGCCA CAACGAGGAT 
CTCATCAATA CGGAACTTCA GAAGAAGCTT CGCATCCGAT TCTCGACGTC TGTGATGGGC 
ACGTCTGCGT GCAAAGCCCA CCTGCTCTTC CAGGCTCATT TCATGCGGAC TGTGCTCCCG 
ACGGATTACC GGACTGATTT GAAGAGTGTG CTGGATCAGT GCATTCGGAT TTTGCAGGCG 
ATGCGTGAAA TGGCTAGGCT CAAGAATTGG CTCAGCGCAA CGATGAACAT TATTCTGTTG 
CAGCAGATGT GCCATTCGGC AAGGTGGCAC GACGATCATC CATTGCTCTG CCTACCTCAT 
CTCTCCCACG AGGATGCTCG TTCAATCGGC GATGGAATGA CAATTCCACA GCTTCAAAAC 
CATCTGGAAA TCGAGAAATC CACGTCATTA GACGACGCGA AACTTGCTCG ACGAGCCCAG 
AAACTCTTCA GAGAGTGCAC CAAGTTAGAT GAAGCTCAAA GCCGGGAAGT GCTGAAAGCT 
CTGTGCAATT GGCCAATTAT CAACATGAAA ATCATGCAAT TAGTGGATTC CCGCGGTAAT 
TGTGTAGATA TTGATGAGAC GAAGAAACCG GTGAAAGTGA CGGCTGGCGA GGTGTACAAA 
TTGAGAATTG TCATGGAACG AGTCGGACCG GGAAAGAACA ACTCATCAAT GCATCTTCCA 
CAATGGCCAA AACCAAAACA GGCTGGATGG ATAATTGTTG TGGGAAACGT GTCGGCCGAC 
ATGATTCTTA ACACGACTAC CGTAACCGGC TCACATTCGA CTCGGTCGAC CGCCAAATTG 
GATATTCGTG CGCCGGCGAC GAAAGGAAAC CACGAGCTCG CCGTCCTAAT TCTCAGCGAT 
TGCTATTTGG GAATCGATCA GGAGTACACT TTGCGATTGG ATGTCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293536.1
CDS	1..1431
Translation	MMGRAGRPQFDDSAVAVIYVQDAKKTFYKKFLYEPFPVESSLLPVLPNHVNAEISAGTIDSKQAIVEYLSKTYLYRRLFANPNYYGLEEDSEEAMLKFITKIVDDSVAELLASECIHVDSEQDVIKPTPCGRIASVYYLQHETVRFLVKSLHSGCSVENMLKILTDVPEYAEIPVRHNEDLINTELQKKLRIRFSTSVMGTSACKAHLLFQAHFMRTVLPTDYRTDLKSVLDQCIRILQAMREMARLKNWLSATMNIILLQQMCHSARWHDDHPLLCLPHLSHEDARSIGDGMTIPQLQNHLEIEKSTSLDDAKLARRAQKLFRECTKLDEAQSREVLKALCNWPIINMKIMQLVDSRGNCVDIDETKKPVKVTAGEVYKLRIVMERVGPGKNNSSMHLPQWPKPKQAGWIIVVGNVSADMILNTTTVTGSHSTRSTAKLDIRAPATKGNHELAVLILSDCYLGIDQEYTLRLDVC

Target ORF information:

RefSeq Version	NM_001306607.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (Y54E2A.4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306607.1

ORF Insert Sequence:

ATGATGGGCC GCGCCGGACG CCCACAATTC GACGATTCGG CCGTTGCCGT GATCTACGTA 
CAGGACGCGA AAAAGACGTT CTATAAGAAG TTCCTCTACG AACCGTTCCC CGTCGAGTCC 
TCGCTCCTTC CAGTGCTGCC GAATCACGTG AACGCCGAGA TTTCCGCCGG AACAATCGAT 
TCGAAGCAGG CGATCGTCGA GTATTTGTCG AAAACCTATC TCTACAGGCG ATTGTTTGCG 
AATCCCAATT ATTACGGTCT GGAGGAGGAT TCCGAGGAGG CGATGCTCAA GTTTATCACG 
AAAATCGTTG ATGACTCTGT CGCAGAGCTC CTAGCTTCCG AGTGTATCCA CGTGGATTCC 
GAACAGGACG TGATCAAGCC GACGCCGTGT GGAAGGATAG CTTCGGTGTA CTATCTTCAG 
CACGAGACCG TCCGTTTTCT AGTAAAATCT CTACATTCTG GATGCTCGGT GGAGAATATG 
CTGAAGATCC TGACCGATGT TCCCGAGTAC GCGGAGATTC CTGTCCGCCA CAACGAGGAT 
CTCATCAATA CGGAACTTCA GAAGAAGCTT CGCATCCGAT TCTCGACGTC TGTGATGGGC 
ACGTCTGCGT GCAAAGCCCA CCTGCTCTTC CAGGCTCATT TCATGCGGAC TGTGCTCCCG 
ACGGATTACC GGACTGATTT GAAGAGTGTG CTGGATCAGT GCATTCGGAT TTTGCAGGCG 
ATGCGTGAAA TGGCTAGGCT CAAGAATTGG CTCAGCGCAA CGATGAACAT TATTCTGTTG 
CAGCAGATGT GCCATTCGGC AAGGTGGCAC GACGATCATC CATTGCTCTG CCTACCTCAT 
CTCTCCCACG AGGATGCTCG TTCAATCGGC GATGGAATGA CAATTCCACA GCTTCAAAAC 
CATCTGGAAA TCGAGAAATC CACGTCATTA GACGACGCGA AACTTGCTCG ACGAGCCCAG 
AAACTCTTCA GAGAGTGCAC CAAGTTAGAT GAAGCTCAAA GCCGGGAAGT GCTGAAAGCT 
CTGTGCAATT GGCCAATTAT CAACATGAAA ATCATGCAAT TAGTGGATTC CCGCGGTAAT 
TGTGTAGATA TTGATGAGAC GAAGAAACCG GTGAAAGTGA CGGCTGGCGA GGTGTACAAA 
TTGAGAATTG TCATGGAACG AGTCGGACCG GGAAAGAACA ACTCATCAAT GCATCTTCCA 
CAATGGCCAA AACCAAAACA GGCTGGATGG ATAATTGTTG TGGGAAACGT GTCGGCCGAC 
ATGATTCTTA ACACGACTAC CGTAACCGGC TCACATTCGA CTCGGTCGAC CGCCAAATTG 
GATATTCGTG CGCCGGCGAC GAAAGGAAAC CACGAGCTCG CCGTCCTAAT TCTCAGCGAT 
TGCTATTTGG GAATCGATCA GGAGTACACT TTGCGATTGG ATGTCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Y54E2A.4 ( NM_001306607.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293536.1 Caenorhabditis elegans SEC63 domain-containing protein (Y54E2A.4), partial mRNA.

Price & Availability↑

CloneID	OCa360032
Clone ID Related Accession (Same CDS sequence)	NM_001306607.1 , NM_001306607.2
Accession Version	NM_001306607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SEC63 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001306607.1. COMPLETENESS: incomplete on the 5' end.

ATGATGGGCC GCGCCGGACG CCCACAATTC GACGATTCGG CCGTTGCCGT GATCTACGTA 
CAGGACGCGA AAAAGACGTT CTATAAGAAG TTCCTCTACG AACCGTTCCC CGTCGAGTCC 
TCGCTCCTTC CAGTGCTGCC GAATCACGTG AACGCCGAGA TTTCCGCCGG AACAATCGAT 
TCGAAGCAGG CGATCGTCGA GTATTTGTCG AAAACCTATC TCTACAGGCG ATTGTTTGCG 
AATCCCAATT ATTACGGTCT GGAGGAGGAT TCCGAGGAGG CGATGCTCAA GTTTATCACG 
AAAATCGTTG ATGACTCTGT CGCAGAGCTC CTAGCTTCCG AGTGTATCCA CGTGGATTCC 
GAACAGGACG TGATCAAGCC GACGCCGTGT GGAAGGATAG CTTCGGTGTA CTATCTTCAG 
CACGAGACCG TCCGTTTTCT AGTAAAATCT CTACATTCTG GATGCTCGGT GGAGAATATG 
CTGAAGATCC TGACCGATGT TCCCGAGTAC GCGGAGATTC CTGTCCGCCA CAACGAGGAT 
CTCATCAATA CGGAACTTCA GAAGAAGCTT CGCATCCGAT TCTCGACGTC TGTGATGGGC 
ACGTCTGCGT GCAAAGCCCA CCTGCTCTTC CAGGCTCATT TCATGCGGAC TGTGCTCCCG 
ACGGATTACC GGACTGATTT GAAGAGTGTG CTGGATCAGT GCATTCGGAT TTTGCAGGCG 
ATGCGTGAAA TGGCTAGGCT CAAGAATTGG CTCAGCGCAA CGATGAACAT TATTCTGTTG 
CAGCAGATGT GCCATTCGGC AAGGTGGCAC GACGATCATC CATTGCTCTG CCTACCTCAT 
CTCTCCCACG AGGATGCTCG TTCAATCGGC GATGGAATGA CAATTCCACA GCTTCAAAAC 
CATCTGGAAA TCGAGAAATC CACGTCATTA GACGACGCGA AACTTGCTCG ACGAGCCCAG 
AAACTCTTCA GAGAGTGCAC CAAGTTAGAT GAAGCTCAAA GCCGGGAAGT GCTGAAAGCT 
CTGTGCAATT GGCCAATTAT CAACATGAAA ATCATGCAAT TAGTGGATTC CCGCGGTAAT 
TGTGTAGATA TTGATGAGAC GAAGAAACCG GTGAAAGTGA CGGCTGGCGA GGTGTACAAA 
TTGAGAATTG TCATGGAACG AGTCGGACCG GGAAAGAACA ACTCATCAAT GCATCTTCCA 
CAATGGCCAA AACCAAAACA GGCTGGATGG ATAATTGTTG TGGGAAACGT GTCGGCCGAC 
ATGATTCTTA ACACGACTAC CGTAACCGGC TCACATTCGA CTCGGTCGAC CGCCAAATTG 
GATATTCGTG CGCCGGCGAC GAAAGGAAAC CACGAGCTCG CCGTCCTAAT TCTCAGCGAT 
TGCTATTTGG GAATCGATCA GGAGTACACT TTGCGATTGG ATGTCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293536.1
CDS	1..1431
Translation	MMGRAGRPQFDDSAVAVIYVQDAKKTFYKKFLYEPFPVESSLLPVLPNHVNAEISAGTIDSKQAIVEYLSKTYLYRRLFANPNYYGLEEDSEEAMLKFITKIVDDSVAELLASECIHVDSEQDVIKPTPCGRIASVYYLQHETVRFLVKSLHSGCSVENMLKILTDVPEYAEIPVRHNEDLINTELQKKLRIRFSTSVMGTSACKAHLLFQAHFMRTVLPTDYRTDLKSVLDQCIRILQAMREMARLKNWLSATMNIILLQQMCHSARWHDDHPLLCLPHLSHEDARSIGDGMTIPQLQNHLEIEKSTSLDDAKLARRAQKLFRECTKLDEAQSREVLKALCNWPIINMKIMQLVDSRGNCVDIDETKKPVKVTAGEVYKLRIVMERVGPGKNNSSMHLPQWPKPKQAGWIIVVGNVSADMILNTTTVTGSHSTRSTAKLDIRAPATKGNHELAVLILSDCYLGIDQEYTLRLDVC

Target ORF information:

RefSeq Version	NM_001306607.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SEC63 domain-containing protein (Y54E2A.4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306607.2

ORF Insert Sequence:

ATGATGGGCC GCGCCGGACG CCCACAATTC GACGATTCGG CCGTTGCCGT GATCTACGTA 
CAGGACGCGA AAAAGACGTT CTATAAGAAG TTCCTCTACG AACCGTTCCC CGTCGAGTCC 
TCGCTCCTTC CAGTGCTGCC GAATCACGTG AACGCCGAGA TTTCCGCCGG AACAATCGAT 
TCGAAGCAGG CGATCGTCGA GTATTTGTCG AAAACCTATC TCTACAGGCG ATTGTTTGCG 
AATCCCAATT ATTACGGTCT GGAGGAGGAT TCCGAGGAGG CGATGCTCAA GTTTATCACG 
AAAATCGTTG ATGACTCTGT CGCAGAGCTC CTAGCTTCCG AGTGTATCCA CGTGGATTCC 
GAACAGGACG TGATCAAGCC GACGCCGTGT GGAAGGATAG CTTCGGTGTA CTATCTTCAG 
CACGAGACCG TCCGTTTTCT AGTAAAATCT CTACATTCTG GATGCTCGGT GGAGAATATG 
CTGAAGATCC TGACCGATGT TCCCGAGTAC GCGGAGATTC CTGTCCGCCA CAACGAGGAT 
CTCATCAATA CGGAACTTCA GAAGAAGCTT CGCATCCGAT TCTCGACGTC TGTGATGGGC 
ACGTCTGCGT GCAAAGCCCA CCTGCTCTTC CAGGCTCATT TCATGCGGAC TGTGCTCCCG 
ACGGATTACC GGACTGATTT GAAGAGTGTG CTGGATCAGT GCATTCGGAT TTTGCAGGCG 
ATGCGTGAAA TGGCTAGGCT CAAGAATTGG CTCAGCGCAA CGATGAACAT TATTCTGTTG 
CAGCAGATGT GCCATTCGGC AAGGTGGCAC GACGATCATC CATTGCTCTG CCTACCTCAT 
CTCTCCCACG AGGATGCTCG TTCAATCGGC GATGGAATGA CAATTCCACA GCTTCAAAAC 
CATCTGGAAA TCGAGAAATC CACGTCATTA GACGACGCGA AACTTGCTCG ACGAGCCCAG 
AAACTCTTCA GAGAGTGCAC CAAGTTAGAT GAAGCTCAAA GCCGGGAAGT GCTGAAAGCT 
CTGTGCAATT GGCCAATTAT CAACATGAAA ATCATGCAAT TAGTGGATTC CCGCGGTAAT 
TGTGTAGATA TTGATGAGAC GAAGAAACCG GTGAAAGTGA CGGCTGGCGA GGTGTACAAA 
TTGAGAATTG TCATGGAACG AGTCGGACCG GGAAAGAACA ACTCATCAAT GCATCTTCCA 
CAATGGCCAA AACCAAAACA GGCTGGATGG ATAATTGTTG TGGGAAACGT GTCGGCCGAC 
ATGATTCTTA ACACGACTAC CGTAACCGGC TCACATTCGA CTCGGTCGAC CGCCAAATTG 
GATATTCGTG CGCCGGCGAC GAAAGGAAAC CACGAGCTCG CCGTCCTAAT TCTCAGCGAT 
TGCTATTTGG GAATCGATCA GGAGTACACT TTGCGATTGG ATGTCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Y54E2A.4 ( NM_001306606.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293535.1 Caenorhabditis elegans SEC63 domain-containing protein (Y54E2A.4), mRNA.

Price & Availability↑

CloneID	OCa360031
Clone ID Related Accession (Same CDS sequence)	NM_001306606.2 , NM_001306606.1
Accession Version	NM_001306606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SEC63 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001306606.1.

ATGCTCGTTC ACCAAAACCC GATTGAAAGT CAATTCTACG CTCGGCTTCA CGATAATTTG 
AACGCTGAAG TGGCCCTGGG TACCGTATCC ACAGTGGATG AGGGTGTTGA ATGGTTGACC 
TATACCTACA TGTACACGAG AGCCCTGAAA AATCCGATGG CTTATGGAAT TGCGTATAAT 
GCAATTGAAC GGGATCCGAA TTTGAGAGAT CATTTTGGAA ATGTGATTCG CGAGGCGGCG 
ATGCAACTGG ATCAGAATAA AATGATTCGA TTTGATATGG CAACCGAATA CCTAAACTCG 
ACGGATCTCG GTCGAATCGC CTCCAATTTC TATGTCAAAT ACGAAACAAT TCAGCTCCTC 
AACGAAGCGG AAAAGGGCGT CGGATTACCA GTAACTTTCA CAAGTTTCAT GCCAGACGAC 
ATGGTCATCG GACTCATCTC AATGTCCACC GAGTTCGCGA ATTTGAAGTG TCGTGAGGAG 
GAGATCGGCG ATCTGGAAGA GCTGATGAGC TACGGATGTA TGATGAACGT TCGTGGTGGT 
GGTCTGGCGA GTGTCGCCGG AAAAGTCAAT GTTCTCCTTC AATCACTGAT CTCTAGAACA 
TCGACGAGAA ACTCGGCGCT CATGTCCGAG CAGCTATACG TTCAGCAGAA CGCCGGTCGG 
CTCTGCAGAG CTATGTTTGA GATGGTTCTG AAGAACGGGT GGAGCCAGGC GGCCAATGCC 
TTTTTGGGAA TTGCAAAATG TGTCGAGAAG CAGATGTGGA TGAATCAGTG CGCGTTGCGG 
CAGTTTATTC AGATCATCAA CATCCCCATC ACATGGATCG AGAAAATCGA GCGTAAAAAA 
GCGAGAGAAT CGGATTTACT CGAGCTCTCC GCCAAAGATC TCGGCTACAT GTTCAGTTGC 
GACGGTGACC GCCTATACAC CTACCTACGC TACCTTCCGA GGATGGATGT GCAAGCAAAG 
TTCAAGCCGA TCACCTACAC GATCGTTCAG GTCGAGGTCA CACTCACGCC GACGTTCATC 
TGGAACGACC AGATCCACGG AAAGTCGGGA CAACAATCGT TCTATCTAGT GCTGGAGAAT 
CTCAACGAGA ATCTTATCAT CCATCAGGAG AGAATCGGCA TCGGGAAGAT GAAGGTGAAC 
CGGCAGGAGA CTCAGAATCT AGTTTTCACT ATTCCAATCG TCGACTGTCA GCTGACGAAC 
AATTTCCAAC TTCGCCTCGC CAGCGAATAC TTTGTCACCG ACGACGTGGT GGTTCCGATG 
TCTCTTCACA ATTGCATTCT ACCAAAGTCC TTCAAAAGCC ACACAGATCT TCTGGACCTT 
GAGCCACTTC CTATCAAAAC TCTCAAAAAC TCAAAGTTCG AGGCGATCTA CAACTTTGAC 
TATTTCAACC CAATTCAAGC GCAAGTTTTC TTTTGCCTGT ACAAAACCGA CAAAAGCGCA 
TTGATCGGAG CGCCGACAGG ATCTGGAAAG ACACTTTGCG CGGAGCTCGC CATGTTCCGC 
CTGCTTCAGG ACCATCCTGG CATGAAAGTC GTGTACATTG CTCCGTTGAA GTCCTTGGTC 
CGAGAGCGAG TTGACGATTG GAAGAAGAAG TTCGAAGATG GAATGGGATA TCGAGTGGTC 
GAGGTGTCTG GGGATGTGAC TCCAGATCCC GAGGAGCTTG CGGCTTCCTC AATTCTTATT 
ACGACACCAG AAAAGTGGGA TGGAATCTCC AGAAGTTGGG CTACGAGGGA ATACGTCCGA 
CGAGTCGGCC TTATCGTGCT CGACGAGGTT CACCTACTCG GCGTAGATCG TGGAGCTGTG 
CTCGAGGCCA TCGTCAGCAG ACTGAAAATG ATCACCAGAA GAAGCCACGT GACAGAGGAG 
CCTGTCAGGT TGCTGGGTCT TTCCACAGCG CTCGCCAACG CAGGAGACGT CGCAGAGTGG 
CTTGGCATCC CCGACGAGGC GTGCTACAAC TTCCGACCTT CAGTTCGACC AGTGCCGATT 
TCAGTTCATA TTCAAGGATT CCCAGGCCAA CATTACTGCC CACGAATGGG ACTGATGAAC 
AAGCCGGCTT ACAAGGCAAT TCTGACCTAT TCCCCAAGGA AGCCTGTGTT GATCTTCGTC 
TCGTCTAGAC GACAAACCCG CCTGACAGCG CTCGCCTTTG TCAATTTGCT CATTGCCGAC 
CACAATCCGA AGCAATGGCT CAACATGGAC ATGCTCGAGT TGGAAGATCT CATGGCCGCA 
ATCAAGGATG AAAATCTCAA GTTGACACTA CCATTCGGAA TCGGAATGCA TCACGCTGGA 
CTCAGCGCTC ACGAGCGAGC CATTGTGGAG CAATTGTTTA TCGAAAAGAA GATTCAAGTG 
CTCATCGCGA CTGCCACATT GGCGTGGGGA ATCAATTGCC CCGCTCATTT AGTTATTGTC 
AAGGGAACCG AATATTTCGA CGGAAAGAAG GGAAAATACG TGGATTTTCC CGTTACAGAC 
GTTCTCCAAA TGATGGGCCG CGCCGGACGC CCACAATTCG ACGATTCGGC CGTTGCCGTG 
ATCTACGTAC AGGACGCGAA AAAGACGTTC TATAAGAAGT TCCTCTACGA ACCGTTCCCC 
GTCGAGTCCT CGCTCCTTCC AGTGCTGCCG AATCACGTGA ACGCCGAGAT TTCCGCCGGA 
ACAATCGATT CGAAGCAGGC GATCGTCGAG TATTTGTCGA AAACCTATCT CTACAGGCGA 
TTGTTTGCGA ATCCCAATTA TTACGGTCTG GAGGAGGATT CCGAGGAGGC GATGCTCAAG 
TTTATCACGA AAATCGTTGA TGACTCTGTC GCAGAGCTCC TAGCTTCCGA GTGTATCCAC 
GTGGATTCCG AACAGGACGT GATCAAGCCG ACGCCGTGTG GAAGGATAGC TTCGGTGTAC 
TATCTTCAGC ACGAGACCGT CCGTTTTCTA GTAAAATCTC TACATTCTGG ATGCTCGGTG 
GAGAATATGC TGAAGATCCT GACCGATGTT CCCGAGTACG CGGAGATTCC TGTCCGCCAC 
AACGAGGATC TCATCAATAC GGAACTTCAG AAGAAGCTTC GCATCCGATT CTCGACGTCT 
GTGATGGGCA CGTCTGCGTG CAAAGCCCAC CTGCTCTTCC AGGCTCATTT CATGCGGACT 
GTGCTCCCGA CGGATTACCG GACTGATTTG AAGAGTGTGC TGGATCAGTG CATTCGGATT 
TTGCAGGCGA TGCGTGAAAT GGCTAGGCTC AAGAATTGGC TCAGCGCAAC GATGAACATT 
ATTCTGTTGC AGCAGATGTG CCATTCGGCA AGGTGGCACG ACGATCATCC ATTGCTCTGC 
CTACCTCATC TCTCCCACGA GGATGCTCGT TCAATCGGCG ATGGAATGAC AATTCCACAG 
CTTCAAAACC ATCTGGAAAT CGAGAAATCC ACGTCATTAG ACGACGCGAA ACTTGCTCGA 
CGAGCCCAGA AACTCTTCAG AGAGTGCACC AAGTTAGATG AAGCTCAAAG CCGGGAAGTG 
CTGAAAGCTC TGTGCAATTG GCCAATTATC AACATGAAAA TCATGCAATT AGTGGATTCC 
CGCGGTAATT GTGTAGATAT TGATGAGACG AAGAAACCGG TGAAAGTGAC GGCTGGCGAG 
GTGTACAAAT TGAGAATTGT CATGGAACGA GTCGGACCGG GAAAGAACAA CTCATCAATG 
CATCTTCCAC AATGGCCAAA ACCAAAACAG GCTGGATGGA TAATTGTTGT GGGAAACGTG 
TCGGCCGACA TGATTCTTAA CACGACTACC GTAACCGGCT CACATTCGAC TCGGTCGACC 
GCCAAATTGG ATATTCGTGC GCCGGCGACG AAAGGAAACC ACGAGCTCGC CGTCCTAATT 
CTCAGCGATT GCTATTTGGG AATCGATCAG GAGTACACTT TGCGATTGGA TGTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293535.1
CDS	3..3902
Translation	MLVHQNPIESQFYARLHDNLNAEVALGTVSTVDEGVEWLTYTYMYTRALKNPMAYGIAYNAIERDPNLRDHFGNVIREAAMQLDQNKMIRFDMATEYLNSTDLGRIASNFYVKYETIQLLNEAEKGVGLPVTFTSFMPDDMVIGLISMSTEFANLKCREEEIGDLEELMSYGCMMNVRGGGLASVAGKVNVLLQSLISRTSTRNSALMSEQLYVQQNAGRLCRAMFEMVLKNGWSQAANAFLGIAKCVEKQMWMNQCALRQFIQIINIPITWIEKIERKKARESDLLELSAKDLGYMFSCDGDRLYTYLRYLPRMDVQAKFKPITYTIVQVEVTLTPTFIWNDQIHGKSGQQSFYLVLENLNENLIIHQERIGIGKMKVNRQETQNLVFTIPIVDCQLTNNFQLRLASEYFVTDDVVVPMSLHNCILPKSFKSHTDLLDLEPLPIKTLKNSKFEAIYNFDYFNPIQAQVFFCLYKTDKSALIGAPTGSGKTLCAELAMFRLLQDHPGMKVVYIAPLKSLVRERVDDWKKKFEDGMGYRVVEVSGDVTPDPEELAASSILITTPEKWDGISRSWATREYVRRVGLIVLDEVHLLGVDRGAVLEAIVSRLKMITRRSHVTEEPVRLLGLSTALANAGDVAEWLGIPDEACYNFRPSVRPVPISVHIQGFPGQHYCPRMGLMNKPAYKAILTYSPRKPVLIFVSSRRQTRLTALAFVNLLIADHNPKQWLNMDMLELEDLMAAIKDENLKLTLPFGIGMHHAGLSAHERAIVEQLFIEKKIQVLIATATLAWGINCPAHLVIVKGTEYFDGKKGKYVDFPVTDVLQMMGRAGRPQFDDSAVAVIYVQDAKKTFYKKFLYEPFPVESSLLPVLPNHVNAEISAGTIDSKQAIVEYLSKTYLYRRLFANPNYYGLEEDSEEAMLKFITKIVDDSVAELLASECIHVDSEQDVIKPTPCGRIASVYYLQHETVRFLVKSLHSGCSVENMLKILTDVPEYAEIPVRHNEDLINTELQKKLRIRFSTSVMGTSACKAHLLFQAHFMRTVLPTDYRTDLKSVLDQCIRILQAMREMARLKNWLSATMNIILLQQMCHSARWHDDHPLLCLPHLSHEDARSIGDGMTIPQLQNHLEIEKSTSLDDAKLARRAQKLFRECTKLDEAQSREVLKALCNWPIINMKIMQLVDSRGNCVDIDETKKPVKVTAGEVYKLRIVMERVGPGKNNSSMHLPQWPKPKQAGWIIVVGNVSADMILNTTTVTGSHSTRSTAKLDIRAPATKGNHELAVLILSDCYLGIDQEYTLRLDVC

Target ORF information:

RefSeq Version	NM_001306606.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SEC63 domain-containing protein (Y54E2A.4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306606.2

ORF Insert Sequence:

ATGCTCGTTC ACCAAAACCC GATTGAAAGT CAATTCTACG CTCGGCTTCA CGATAATTTG 
AACGCTGAAG TGGCCCTGGG TACCGTATCC ACAGTGGATG AGGGTGTTGA ATGGTTGACC 
TATACCTACA TGTACACGAG AGCCCTGAAA AATCCGATGG CTTATGGAAT TGCGTATAAT 
GCAATTGAAC GGGATCCGAA TTTGAGAGAT CATTTTGGAA ATGTGATTCG CGAGGCGGCG 
ATGCAACTGG ATCAGAATAA AATGATTCGA TTTGATATGG CAACCGAATA CCTAAACTCG 
ACGGATCTCG GTCGAATCGC CTCCAATTTC TATGTCAAAT ACGAAACAAT TCAGCTCCTC 
AACGAAGCGG AAAAGGGCGT CGGATTACCA GTAACTTTCA CAAGTTTCAT GCCAGACGAC 
ATGGTCATCG GACTCATCTC AATGTCCACC GAGTTCGCGA ATTTGAAGTG TCGTGAGGAG 
GAGATCGGCG ATCTGGAAGA GCTGATGAGC TACGGATGTA TGATGAACGT TCGTGGTGGT 
GGTCTGGCGA GTGTCGCCGG AAAAGTCAAT GTTCTCCTTC AATCACTGAT CTCTAGAACA 
TCGACGAGAA ACTCGGCGCT CATGTCCGAG CAGCTATACG TTCAGCAGAA CGCCGGTCGG 
CTCTGCAGAG CTATGTTTGA GATGGTTCTG AAGAACGGGT GGAGCCAGGC GGCCAATGCC 
TTTTTGGGAA TTGCAAAATG TGTCGAGAAG CAGATGTGGA TGAATCAGTG CGCGTTGCGG 
CAGTTTATTC AGATCATCAA CATCCCCATC ACATGGATCG AGAAAATCGA GCGTAAAAAA 
GCGAGAGAAT CGGATTTACT CGAGCTCTCC GCCAAAGATC TCGGCTACAT GTTCAGTTGC 
GACGGTGACC GCCTATACAC CTACCTACGC TACCTTCCGA GGATGGATGT GCAAGCAAAG 
TTCAAGCCGA TCACCTACAC GATCGTTCAG GTCGAGGTCA CACTCACGCC GACGTTCATC 
TGGAACGACC AGATCCACGG AAAGTCGGGA CAACAATCGT TCTATCTAGT GCTGGAGAAT 
CTCAACGAGA ATCTTATCAT CCATCAGGAG AGAATCGGCA TCGGGAAGAT GAAGGTGAAC 
CGGCAGGAGA CTCAGAATCT AGTTTTCACT ATTCCAATCG TCGACTGTCA GCTGACGAAC 
AATTTCCAAC TTCGCCTCGC CAGCGAATAC TTTGTCACCG ACGACGTGGT GGTTCCGATG 
TCTCTTCACA ATTGCATTCT ACCAAAGTCC TTCAAAAGCC ACACAGATCT TCTGGACCTT 
GAGCCACTTC CTATCAAAAC TCTCAAAAAC TCAAAGTTCG AGGCGATCTA CAACTTTGAC 
TATTTCAACC CAATTCAAGC GCAAGTTTTC TTTTGCCTGT ACAAAACCGA CAAAAGCGCA 
TTGATCGGAG CGCCGACAGG ATCTGGAAAG ACACTTTGCG CGGAGCTCGC CATGTTCCGC 
CTGCTTCAGG ACCATCCTGG CATGAAAGTC GTGTACATTG CTCCGTTGAA GTCCTTGGTC 
CGAGAGCGAG TTGACGATTG GAAGAAGAAG TTCGAAGATG GAATGGGATA TCGAGTGGTC 
GAGGTGTCTG GGGATGTGAC TCCAGATCCC GAGGAGCTTG CGGCTTCCTC AATTCTTATT 
ACGACACCAG AAAAGTGGGA TGGAATCTCC AGAAGTTGGG CTACGAGGGA ATACGTCCGA 
CGAGTCGGCC TTATCGTGCT CGACGAGGTT CACCTACTCG GCGTAGATCG TGGAGCTGTG 
CTCGAGGCCA TCGTCAGCAG ACTGAAAATG ATCACCAGAA GAAGCCACGT GACAGAGGAG 
CCTGTCAGGT TGCTGGGTCT TTCCACAGCG CTCGCCAACG CAGGAGACGT CGCAGAGTGG 
CTTGGCATCC CCGACGAGGC GTGCTACAAC TTCCGACCTT CAGTTCGACC AGTGCCGATT 
TCAGTTCATA TTCAAGGATT CCCAGGCCAA CATTACTGCC CACGAATGGG ACTGATGAAC 
AAGCCGGCTT ACAAGGCAAT TCTGACCTAT TCCCCAAGGA AGCCTGTGTT GATCTTCGTC 
TCGTCTAGAC GACAAACCCG CCTGACAGCG CTCGCCTTTG TCAATTTGCT CATTGCCGAC 
CACAATCCGA AGCAATGGCT CAACATGGAC ATGCTCGAGT TGGAAGATCT CATGGCCGCA 
ATCAAGGATG AAAATCTCAA GTTGACACTA CCATTCGGAA TCGGAATGCA TCACGCTGGA 
CTCAGCGCTC ACGAGCGAGC CATTGTGGAG CAATTGTTTA TCGAAAAGAA GATTCAAGTG 
CTCATCGCGA CTGCCACATT GGCGTGGGGA ATCAATTGCC CCGCTCATTT AGTTATTGTC 
AAGGGAACCG AATATTTCGA CGGAAAGAAG GGAAAATACG TGGATTTTCC CGTTACAGAC 
GTTCTCCAAA TGATGGGCCG CGCCGGACGC CCACAATTCG ACGATTCGGC CGTTGCCGTG 
ATCTACGTAC AGGACGCGAA AAAGACGTTC TATAAGAAGT TCCTCTACGA ACCGTTCCCC 
GTCGAGTCCT CGCTCCTTCC AGTGCTGCCG AATCACGTGA ACGCCGAGAT TTCCGCCGGA 
ACAATCGATT CGAAGCAGGC GATCGTCGAG TATTTGTCGA AAACCTATCT CTACAGGCGA 
TTGTTTGCGA ATCCCAATTA TTACGGTCTG GAGGAGGATT CCGAGGAGGC GATGCTCAAG 
TTTATCACGA AAATCGTTGA TGACTCTGTC GCAGAGCTCC TAGCTTCCGA GTGTATCCAC 
GTGGATTCCG AACAGGACGT GATCAAGCCG ACGCCGTGTG GAAGGATAGC TTCGGTGTAC 
TATCTTCAGC ACGAGACCGT CCGTTTTCTA GTAAAATCTC TACATTCTGG ATGCTCGGTG 
GAGAATATGC TGAAGATCCT GACCGATGTT CCCGAGTACG CGGAGATTCC TGTCCGCCAC 
AACGAGGATC TCATCAATAC GGAACTTCAG AAGAAGCTTC GCATCCGATT CTCGACGTCT 
GTGATGGGCA CGTCTGCGTG CAAAGCCCAC CTGCTCTTCC AGGCTCATTT CATGCGGACT 
GTGCTCCCGA CGGATTACCG GACTGATTTG AAGAGTGTGC TGGATCAGTG CATTCGGATT 
TTGCAGGCGA TGCGTGAAAT GGCTAGGCTC AAGAATTGGC TCAGCGCAAC GATGAACATT 
ATTCTGTTGC AGCAGATGTG CCATTCGGCA AGGTGGCACG ACGATCATCC ATTGCTCTGC 
CTACCTCATC TCTCCCACGA GGATGCTCGT TCAATCGGCG ATGGAATGAC AATTCCACAG 
CTTCAAAACC ATCTGGAAAT CGAGAAATCC ACGTCATTAG ACGACGCGAA ACTTGCTCGA 
CGAGCCCAGA AACTCTTCAG AGAGTGCACC AAGTTAGATG AAGCTCAAAG CCGGGAAGTG 
CTGAAAGCTC TGTGCAATTG GCCAATTATC AACATGAAAA TCATGCAATT AGTGGATTCC 
CGCGGTAATT GTGTAGATAT TGATGAGACG AAGAAACCGG TGAAAGTGAC GGCTGGCGAG 
GTGTACAAAT TGAGAATTGT CATGGAACGA GTCGGACCGG GAAAGAACAA CTCATCAATG 
CATCTTCCAC AATGGCCAAA ACCAAAACAG GCTGGATGGA TAATTGTTGT GGGAAACGTG 
TCGGCCGACA TGATTCTTAA CACGACTACC GTAACCGGCT CACATTCGAC TCGGTCGACC 
GCCAAATTGG ATATTCGTGC GCCGGCGACG AAAGGAAACC ACGAGCTCGC CGTCCTAATT 
CTCAGCGATT GCTATTTGGG AATCGATCAG GAGTACACTT TGCGATTGGA TGTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Y54E2A.4 ( NM_001306606.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001293535.1 Caenorhabditis elegans Uncharacterized protein (Y54E2A.4), partial mRNA.

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CloneID	OCa360031
Clone ID Related Accession (Same CDS sequence)	NM_001306606.2 , NM_001306606.1
Accession Version	NM_001306606.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-09
Organism	Caenorhabditis elegans
Product	hypothetical protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGCTCGTTC ACCAAAACCC GATTGAAAGT CAATTCTACG CTCGGCTTCA CGATAATTTG 
AACGCTGAAG TGGCCCTGGG TACCGTATCC ACAGTGGATG AGGGTGTTGA ATGGTTGACC 
TATACCTACA TGTACACGAG AGCCCTGAAA AATCCGATGG CTTATGGAAT TGCGTATAAT 
GCAATTGAAC GGGATCCGAA TTTGAGAGAT CATTTTGGAA ATGTGATTCG CGAGGCGGCG 
ATGCAACTGG ATCAGAATAA AATGATTCGA TTTGATATGG CAACCGAATA CCTAAACTCG 
ACGGATCTCG GTCGAATCGC CTCCAATTTC TATGTCAAAT ACGAAACAAT TCAGCTCCTC 
AACGAAGCGG AAAAGGGCGT CGGATTACCA GTAACTTTCA CAAGTTTCAT GCCAGACGAC 
ATGGTCATCG GACTCATCTC AATGTCCACC GAGTTCGCGA ATTTGAAGTG TCGTGAGGAG 
GAGATCGGCG ATCTGGAAGA GCTGATGAGC TACGGATGTA TGATGAACGT TCGTGGTGGT 
GGTCTGGCGA GTGTCGCCGG AAAAGTCAAT GTTCTCCTTC AATCACTGAT CTCTAGAACA 
TCGACGAGAA ACTCGGCGCT CATGTCCGAG CAGCTATACG TTCAGCAGAA CGCCGGTCGG 
CTCTGCAGAG CTATGTTTGA GATGGTTCTG AAGAACGGGT GGAGCCAGGC GGCCAATGCC 
TTTTTGGGAA TTGCAAAATG TGTCGAGAAG CAGATGTGGA TGAATCAGTG CGCGTTGCGG 
CAGTTTATTC AGATCATCAA CATCCCCATC ACATGGATCG AGAAAATCGA GCGTAAAAAA 
GCGAGAGAAT CGGATTTACT CGAGCTCTCC GCCAAAGATC TCGGCTACAT GTTCAGTTGC 
GACGGTGACC GCCTATACAC CTACCTACGC TACCTTCCGA GGATGGATGT GCAAGCAAAG 
TTCAAGCCGA TCACCTACAC GATCGTTCAG GTCGAGGTCA CACTCACGCC GACGTTCATC 
TGGAACGACC AGATCCACGG AAAGTCGGGA CAACAATCGT TCTATCTAGT GCTGGAGAAT 
CTCAACGAGA ATCTTATCAT CCATCAGGAG AGAATCGGCA TCGGGAAGAT GAAGGTGAAC 
CGGCAGGAGA CTCAGAATCT AGTTTTCACT ATTCCAATCG TCGACTGTCA GCTGACGAAC 
AATTTCCAAC TTCGCCTCGC CAGCGAATAC TTTGTCACCG ACGACGTGGT GGTTCCGATG 
TCTCTTCACA ATTGCATTCT ACCAAAGTCC TTCAAAAGCC ACACAGATCT TCTGGACCTT 
GAGCCACTTC CTATCAAAAC TCTCAAAAAC TCAAAGTTCG AGGCGATCTA CAACTTTGAC 
TATTTCAACC CAATTCAAGC GCAAGTTTTC TTTTGCCTGT ACAAAACCGA CAAAAGCGCA 
TTGATCGGAG CGCCGACAGG ATCTGGAAAG ACACTTTGCG CGGAGCTCGC CATGTTCCGC 
CTGCTTCAGG ACCATCCTGG CATGAAAGTC GTGTACATTG CTCCGTTGAA GTCCTTGGTC 
CGAGAGCGAG TTGACGATTG GAAGAAGAAG TTCGAAGATG GAATGGGATA TCGAGTGGTC 
GAGGTGTCTG GGGATGTGAC TCCAGATCCC GAGGAGCTTG CGGCTTCCTC AATTCTTATT 
ACGACACCAG AAAAGTGGGA TGGAATCTCC AGAAGTTGGG CTACGAGGGA ATACGTCCGA 
CGAGTCGGCC TTATCGTGCT CGACGAGGTT CACCTACTCG GCGTAGATCG TGGAGCTGTG 
CTCGAGGCCA TCGTCAGCAG ACTGAAAATG ATCACCAGAA GAAGCCACGT GACAGAGGAG 
CCTGTCAGGT TGCTGGGTCT TTCCACAGCG CTCGCCAACG CAGGAGACGT CGCAGAGTGG 
CTTGGCATCC CCGACGAGGC GTGCTACAAC TTCCGACCTT CAGTTCGACC AGTGCCGATT 
TCAGTTCATA TTCAAGGATT CCCAGGCCAA CATTACTGCC CACGAATGGG ACTGATGAAC 
AAGCCGGCTT ACAAGGCAAT TCTGACCTAT TCCCCAAGGA AGCCTGTGTT GATCTTCGTC 
TCGTCTAGAC GACAAACCCG CCTGACAGCG CTCGCCTTTG TCAATTTGCT CATTGCCGAC 
CACAATCCGA AGCAATGGCT CAACATGGAC ATGCTCGAGT TGGAAGATCT CATGGCCGCA 
ATCAAGGATG AAAATCTCAA GTTGACACTA CCATTCGGAA TCGGAATGCA TCACGCTGGA 
CTCAGCGCTC ACGAGCGAGC CATTGTGGAG CAATTGTTTA TCGAAAAGAA GATTCAAGTG 
CTCATCGCGA CTGCCACATT GGCGTGGGGA ATCAATTGCC CCGCTCATTT AGTTATTGTC 
AAGGGAACCG AATATTTCGA CGGAAAGAAG GGAAAATACG TGGATTTTCC CGTTACAGAC 
GTTCTCCAAA TGATGGGCCG CGCCGGACGC CCACAATTCG ACGATTCGGC CGTTGCCGTG 
ATCTACGTAC AGGACGCGAA AAAGACGTTC TATAAGAAGT TCCTCTACGA ACCGTTCCCC 
GTCGAGTCCT CGCTCCTTCC AGTGCTGCCG AATCACGTGA ACGCCGAGAT TTCCGCCGGA 
ACAATCGATT CGAAGCAGGC GATCGTCGAG TATTTGTCGA AAACCTATCT CTACAGGCGA 
TTGTTTGCGA ATCCCAATTA TTACGGTCTG GAGGAGGATT CCGAGGAGGC GATGCTCAAG 
TTTATCACGA AAATCGTTGA TGACTCTGTC GCAGAGCTCC TAGCTTCCGA GTGTATCCAC 
GTGGATTCCG AACAGGACGT GATCAAGCCG ACGCCGTGTG GAAGGATAGC TTCGGTGTAC 
TATCTTCAGC ACGAGACCGT CCGTTTTCTA GTAAAATCTC TACATTCTGG ATGCTCGGTG 
GAGAATATGC TGAAGATCCT GACCGATGTT CCCGAGTACG CGGAGATTCC TGTCCGCCAC 
AACGAGGATC TCATCAATAC GGAACTTCAG AAGAAGCTTC GCATCCGATT CTCGACGTCT 
GTGATGGGCA CGTCTGCGTG CAAAGCCCAC CTGCTCTTCC AGGCTCATTT CATGCGGACT 
GTGCTCCCGA CGGATTACCG GACTGATTTG AAGAGTGTGC TGGATCAGTG CATTCGGATT 
TTGCAGGCGA TGCGTGAAAT GGCTAGGCTC AAGAATTGGC TCAGCGCAAC GATGAACATT 
ATTCTGTTGC AGCAGATGTG CCATTCGGCA AGGTGGCACG ACGATCATCC ATTGCTCTGC 
CTACCTCATC TCTCCCACGA GGATGCTCGT TCAATCGGCG ATGGAATGAC AATTCCACAG 
CTTCAAAACC ATCTGGAAAT CGAGAAATCC ACGTCATTAG ACGACGCGAA ACTTGCTCGA 
CGAGCCCAGA AACTCTTCAG AGAGTGCACC AAGTTAGATG AAGCTCAAAG CCGGGAAGTG 
CTGAAAGCTC TGTGCAATTG GCCAATTATC AACATGAAAA TCATGCAATT AGTGGATTCC 
CGCGGTAATT GTGTAGATAT TGATGAGACG AAGAAACCGG TGAAAGTGAC GGCTGGCGAG 
GTGTACAAAT TGAGAATTGT CATGGAACGA GTCGGACCGG GAAAGAACAA CTCATCAATG 
CATCTTCCAC AATGGCCAAA ACCAAAACAG GCTGGATGGA TAATTGTTGT GGGAAACGTG 
TCGGCCGACA TGATTCTTAA CACGACTACC GTAACCGGCT CACATTCGAC TCGGTCGACC 
GCCAAATTGG ATATTCGTGC GCCGGCGACG AAAGGAAACC ACGAGCTCGC CGTCCTAATT 
CTCAGCGATT GCTATTTGGG AATCGATCAG GAGTACACTT TGCGATTGGA TGTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293535.1
CDS	1..3900
Translation	MLVHQNPIESQFYARLHDNLNAEVALGTVSTVDEGVEWLTYTYMYTRALKNPMAYGIAYNAIERDPNLRDHFGNVIREAAMQLDQNKMIRFDMATEYLNSTDLGRIASNFYVKYETIQLLNEAEKGVGLPVTFTSFMPDDMVIGLISMSTEFANLKCREEEIGDLEELMSYGCMMNVRGGGLASVAGKVNVLLQSLISRTSTRNSALMSEQLYVQQNAGRLCRAMFEMVLKNGWSQAANAFLGIAKCVEKQMWMNQCALRQFIQIINIPITWIEKIERKKARESDLLELSAKDLGYMFSCDGDRLYTYLRYLPRMDVQAKFKPITYTIVQVEVTLTPTFIWNDQIHGKSGQQSFYLVLENLNENLIIHQERIGIGKMKVNRQETQNLVFTIPIVDCQLTNNFQLRLASEYFVTDDVVVPMSLHNCILPKSFKSHTDLLDLEPLPIKTLKNSKFEAIYNFDYFNPIQAQVFFCLYKTDKSALIGAPTGSGKTLCAELAMFRLLQDHPGMKVVYIAPLKSLVRERVDDWKKKFEDGMGYRVVEVSGDVTPDPEELAASSILITTPEKWDGISRSWATREYVRRVGLIVLDEVHLLGVDRGAVLEAIVSRLKMITRRSHVTEEPVRLLGLSTALANAGDVAEWLGIPDEACYNFRPSVRPVPISVHIQGFPGQHYCPRMGLMNKPAYKAILTYSPRKPVLIFVSSRRQTRLTALAFVNLLIADHNPKQWLNMDMLELEDLMAAIKDENLKLTLPFGIGMHHAGLSAHERAIVEQLFIEKKIQVLIATATLAWGINCPAHLVIVKGTEYFDGKKGKYVDFPVTDVLQMMGRAGRPQFDDSAVAVIYVQDAKKTFYKKFLYEPFPVESSLLPVLPNHVNAEISAGTIDSKQAIVEYLSKTYLYRRLFANPNYYGLEEDSEEAMLKFITKIVDDSVAELLASECIHVDSEQDVIKPTPCGRIASVYYLQHETVRFLVKSLHSGCSVENMLKILTDVPEYAEIPVRHNEDLINTELQKKLRIRFSTSVMGTSACKAHLLFQAHFMRTVLPTDYRTDLKSVLDQCIRILQAMREMARLKNWLSATMNIILLQQMCHSARWHDDHPLLCLPHLSHEDARSIGDGMTIPQLQNHLEIEKSTSLDDAKLARRAQKLFRECTKLDEAQSREVLKALCNWPIINMKIMQLVDSRGNCVDIDETKKPVKVTAGEVYKLRIVMERVGPGKNNSSMHLPQWPKPKQAGWIIVVGNVSADMILNTTTVTGSHSTRSTAKLDIRAPATKGNHELAVLILSDCYLGIDQEYTLRLDVC

Target ORF information:

RefSeq Version	NM_001306606.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (Y54E2A.4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306606.1

ORF Insert Sequence:

ATGCTCGTTC ACCAAAACCC GATTGAAAGT CAATTCTACG CTCGGCTTCA CGATAATTTG 
AACGCTGAAG TGGCCCTGGG TACCGTATCC ACAGTGGATG AGGGTGTTGA ATGGTTGACC 
TATACCTACA TGTACACGAG AGCCCTGAAA AATCCGATGG CTTATGGAAT TGCGTATAAT 
GCAATTGAAC GGGATCCGAA TTTGAGAGAT CATTTTGGAA ATGTGATTCG CGAGGCGGCG 
ATGCAACTGG ATCAGAATAA AATGATTCGA TTTGATATGG CAACCGAATA CCTAAACTCG 
ACGGATCTCG GTCGAATCGC CTCCAATTTC TATGTCAAAT ACGAAACAAT TCAGCTCCTC 
AACGAAGCGG AAAAGGGCGT CGGATTACCA GTAACTTTCA CAAGTTTCAT GCCAGACGAC 
ATGGTCATCG GACTCATCTC AATGTCCACC GAGTTCGCGA ATTTGAAGTG TCGTGAGGAG 
GAGATCGGCG ATCTGGAAGA GCTGATGAGC TACGGATGTA TGATGAACGT TCGTGGTGGT 
GGTCTGGCGA GTGTCGCCGG AAAAGTCAAT GTTCTCCTTC AATCACTGAT CTCTAGAACA 
TCGACGAGAA ACTCGGCGCT CATGTCCGAG CAGCTATACG TTCAGCAGAA CGCCGGTCGG 
CTCTGCAGAG CTATGTTTGA GATGGTTCTG AAGAACGGGT GGAGCCAGGC GGCCAATGCC 
TTTTTGGGAA TTGCAAAATG TGTCGAGAAG CAGATGTGGA TGAATCAGTG CGCGTTGCGG 
CAGTTTATTC AGATCATCAA CATCCCCATC ACATGGATCG AGAAAATCGA GCGTAAAAAA 
GCGAGAGAAT CGGATTTACT CGAGCTCTCC GCCAAAGATC TCGGCTACAT GTTCAGTTGC 
GACGGTGACC GCCTATACAC CTACCTACGC TACCTTCCGA GGATGGATGT GCAAGCAAAG 
TTCAAGCCGA TCACCTACAC GATCGTTCAG GTCGAGGTCA CACTCACGCC GACGTTCATC 
TGGAACGACC AGATCCACGG AAAGTCGGGA CAACAATCGT TCTATCTAGT GCTGGAGAAT 
CTCAACGAGA ATCTTATCAT CCATCAGGAG AGAATCGGCA TCGGGAAGAT GAAGGTGAAC 
CGGCAGGAGA CTCAGAATCT AGTTTTCACT ATTCCAATCG TCGACTGTCA GCTGACGAAC 
AATTTCCAAC TTCGCCTCGC CAGCGAATAC TTTGTCACCG ACGACGTGGT GGTTCCGATG 
TCTCTTCACA ATTGCATTCT ACCAAAGTCC TTCAAAAGCC ACACAGATCT TCTGGACCTT 
GAGCCACTTC CTATCAAAAC TCTCAAAAAC TCAAAGTTCG AGGCGATCTA CAACTTTGAC 
TATTTCAACC CAATTCAAGC GCAAGTTTTC TTTTGCCTGT ACAAAACCGA CAAAAGCGCA 
TTGATCGGAG CGCCGACAGG ATCTGGAAAG ACACTTTGCG CGGAGCTCGC CATGTTCCGC 
CTGCTTCAGG ACCATCCTGG CATGAAAGTC GTGTACATTG CTCCGTTGAA GTCCTTGGTC 
CGAGAGCGAG TTGACGATTG GAAGAAGAAG TTCGAAGATG GAATGGGATA TCGAGTGGTC 
GAGGTGTCTG GGGATGTGAC TCCAGATCCC GAGGAGCTTG CGGCTTCCTC AATTCTTATT 
ACGACACCAG AAAAGTGGGA TGGAATCTCC AGAAGTTGGG CTACGAGGGA ATACGTCCGA 
CGAGTCGGCC TTATCGTGCT CGACGAGGTT CACCTACTCG GCGTAGATCG TGGAGCTGTG 
CTCGAGGCCA TCGTCAGCAG ACTGAAAATG ATCACCAGAA GAAGCCACGT GACAGAGGAG 
CCTGTCAGGT TGCTGGGTCT TTCCACAGCG CTCGCCAACG CAGGAGACGT CGCAGAGTGG 
CTTGGCATCC CCGACGAGGC GTGCTACAAC TTCCGACCTT CAGTTCGACC AGTGCCGATT 
TCAGTTCATA TTCAAGGATT CCCAGGCCAA CATTACTGCC CACGAATGGG ACTGATGAAC 
AAGCCGGCTT ACAAGGCAAT TCTGACCTAT TCCCCAAGGA AGCCTGTGTT GATCTTCGTC 
TCGTCTAGAC GACAAACCCG CCTGACAGCG CTCGCCTTTG TCAATTTGCT CATTGCCGAC 
CACAATCCGA AGCAATGGCT CAACATGGAC ATGCTCGAGT TGGAAGATCT CATGGCCGCA 
ATCAAGGATG AAAATCTCAA GTTGACACTA CCATTCGGAA TCGGAATGCA TCACGCTGGA 
CTCAGCGCTC ACGAGCGAGC CATTGTGGAG CAATTGTTTA TCGAAAAGAA GATTCAAGTG 
CTCATCGCGA CTGCCACATT GGCGTGGGGA ATCAATTGCC CCGCTCATTT AGTTATTGTC 
AAGGGAACCG AATATTTCGA CGGAAAGAAG GGAAAATACG TGGATTTTCC CGTTACAGAC 
GTTCTCCAAA TGATGGGCCG CGCCGGACGC CCACAATTCG ACGATTCGGC CGTTGCCGTG 
ATCTACGTAC AGGACGCGAA AAAGACGTTC TATAAGAAGT TCCTCTACGA ACCGTTCCCC 
GTCGAGTCCT CGCTCCTTCC AGTGCTGCCG AATCACGTGA ACGCCGAGAT TTCCGCCGGA 
ACAATCGATT CGAAGCAGGC GATCGTCGAG TATTTGTCGA AAACCTATCT CTACAGGCGA 
TTGTTTGCGA ATCCCAATTA TTACGGTCTG GAGGAGGATT CCGAGGAGGC GATGCTCAAG 
TTTATCACGA AAATCGTTGA TGACTCTGTC GCAGAGCTCC TAGCTTCCGA GTGTATCCAC 
GTGGATTCCG AACAGGACGT GATCAAGCCG ACGCCGTGTG GAAGGATAGC TTCGGTGTAC 
TATCTTCAGC ACGAGACCGT CCGTTTTCTA GTAAAATCTC TACATTCTGG ATGCTCGGTG 
GAGAATATGC TGAAGATCCT GACCGATGTT CCCGAGTACG CGGAGATTCC TGTCCGCCAC 
AACGAGGATC TCATCAATAC GGAACTTCAG AAGAAGCTTC GCATCCGATT CTCGACGTCT 
GTGATGGGCA CGTCTGCGTG CAAAGCCCAC CTGCTCTTCC AGGCTCATTT CATGCGGACT 
GTGCTCCCGA CGGATTACCG GACTGATTTG AAGAGTGTGC TGGATCAGTG CATTCGGATT 
TTGCAGGCGA TGCGTGAAAT GGCTAGGCTC AAGAATTGGC TCAGCGCAAC GATGAACATT 
ATTCTGTTGC AGCAGATGTG CCATTCGGCA AGGTGGCACG ACGATCATCC ATTGCTCTGC 
CTACCTCATC TCTCCCACGA GGATGCTCGT TCAATCGGCG ATGGAATGAC AATTCCACAG 
CTTCAAAACC ATCTGGAAAT CGAGAAATCC ACGTCATTAG ACGACGCGAA ACTTGCTCGA 
CGAGCCCAGA AACTCTTCAG AGAGTGCACC AAGTTAGATG AAGCTCAAAG CCGGGAAGTG 
CTGAAAGCTC TGTGCAATTG GCCAATTATC AACATGAAAA TCATGCAATT AGTGGATTCC 
CGCGGTAATT GTGTAGATAT TGATGAGACG AAGAAACCGG TGAAAGTGAC GGCTGGCGAG 
GTGTACAAAT TGAGAATTGT CATGGAACGA GTCGGACCGG GAAAGAACAA CTCATCAATG 
CATCTTCCAC AATGGCCAAA ACCAAAACAG GCTGGATGGA TAATTGTTGT GGGAAACGTG 
TCGGCCGACA TGATTCTTAA CACGACTACC GTAACCGGCT CACATTCGAC TCGGTCGACC 
GCCAAATTGG ATATTCGTGC GCCGGCGACG AAAGGAAACC ACGAGCTCGC CGTCCTAATT 
CTCAGCGATT GCTATTTGGG AATCGATCAG GAGTACACTT TGCGATTGGA TGTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Y54E2A.4 ( NM_064659.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_497060.2 Caenorhabditis elegans Uncharacterized protein (Y54E2A.4), partial mRNA.

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CloneID	OCa360030
Clone ID Related Accession (Same CDS sequence)	NM_064659.2 , NM_064659.3
Accession Version	NM_064659.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-09
Organism	Caenorhabditis elegans
Product	hypothetical protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_064659.1. COMPLETENESS: incomplete on both ends.

ATGGATCTTC TCAACTATAT CGAACGAATT GGAGATGATC CACCCTCACC ATCACGGGTA 
GCAAGCTTCC ACCAGGAAAT TCAAATTCGA TTTCCATCGG AATTTCTAGA ATGGTCGAAT 
TGTGAAGCTG AACAAGAAGG TTTTTCAGAA GCTCCAGAAG CTCTTTCCCT ACCCCAAGTG 
CCTTCTACCT CGGGCTCTGG GAGCATTTTT CAGCCGATTG CTGACACATT GCTTATATAT 
TTTCCAGATA CGTTTGAGAC AATATCCCAA AACCTTCTAC AAATGTTGTG CTCGACACGT 
TCCGATATGG ATCTACAGGG CGAGCTCATC GATCTTCTCG GCGTCGAATT GTTCGAATTA 
GCCGGAGATA TACTCGAAAA ACGGAATAAA ATAGTTTTCG AGGTGAAGAG CCAGGAGCTG 
GCGAAAGTTG AGCATGCGAG GCAGGCGAAG GCGAAGCCCG CCGTGCCCGG TTTCCTGCAA 
ACTGTCAGCG TACAATCCAC ATCACAAAAC GAGAAACGAC GTGAACTTCG GAAAGAGGAG 
AAGCGAACTC GTCGAGAGCT CAACCGAGTG ATCCATGCGT TCGGCGACGA GGAGAAGCTT 
GAGTTGGAGC TGGCGCAAAA GGAAATCCAA CGCCAACGAC AACTGGAAAT CGATCAAATG 
AAGTGGAAAC CGTCGCTAGT TGCCGGCGGC CCGCGCATCG AGGAAATCTA CCCGTACGTG 
TTCGACGCGA GACTTCAGAA CGTCGGTCAC ACCATGTTCG ACATCAATGG AATGAAGTTC 
GCGCTTCCAG AAGGTTCCAA GCGGGACACC TTTAAGACGC ATGAGAGTGT CTACGTGCCT 
CCGTCGAATA AAGGAGACAT TGAGAAGATT CAGCATGTCT ACGTAAAAGA TATGGATGAG 
CTTGGACAGA AGGGGTTTAA AGGATTTGAA AAACTCAATG TGATTCAATC GATTGTATTC 
GAACAAGCCT ACAAGACCAA GGAGAATCTT CTGATTTGCG CGCCAACCGG AGCCGGAAAG 
ACCAATATTG CGATGCTAAC GATTCTGAAC ACAATCCACG AGCATCAAAA TTCACGAGGA 
GATATTATGA AGGACGACTT TAAAATCATC TACATCGCCC CAATGAAAGC GCTGGCGACC 
GAGATGACCG AGTCATTCGG TAAACGGTTA GCACCGCTTG GCTTGAAAGT GAAGGAGCTC 
ACCGGAGACA CACAACTCTC GAGAAATGAG GTAGCGGATA CTCAGATGTT GGTGCTGACG 
CCTGAGAAAT GGGATGTGAT CACGAGAAAA TCGACCAGTG ACAACTCGCT CATAAATGTG 
GTCCGGCTGC TGATCATCGA CGAAGTTCAT CTGCTTCACG ATGAACGTGG TCCGGTTATT 
GAGACCCTGG TCGCCAGAAC GCTCCGACAG GTCGAGATGT CACAGTCCGG GATTCGAATT 
GTCGGATTGT CGGCGACGCT GCCGAATTTC ATCGATGTCG CCAGATTCTT ACGGGTCAAT 
CCCTACAAGG GCTTGTTCTT CTTCGATGGA AGGTTCAGGC CGGTGCCATT GACTCAGAAA 
TTCATCGGAA CACGAAAAGC GGGCAACTTC CGTGACAATC TCACTCTAAT GGACAACGTG 
TGCTACGACG AAGTGGTGGA TTTTGTGAAA CGCGGCCATC AGGTCCTTGT CTTCGTGCAC 
ACGCGAAACG GAACCGCAAA GCTCGGAGAA GCGTTCTGTG CTCGAGCTTC AGTCCTCGGA 
CAGATGGACC TATTCCTGCC GAAAGATCGA GCGTCGAGCA AGTACATACA AGCGGACAAG 
GCGATCAATA TTTGCCGGAA TCGGGCTCAA ATCTCGCCAC TTTTCCAACG TGGAATGGGA 
ATTCATCACG CTGGACTCTG TCGACAGGAT CGTATTCTCA TGGAGCGATG CTTTGCCGAG 
GGGCATGTTT CTGTGCTGTT CTGTACGGCA ACTCTGGCGT GGGGAGTCAA TTTGCCGGCT 
CATGCGGTTG TTATCAAGGG AACCGATGTT TTTGATGCGG AGAAGGGTGT TTTTACGGAT 
TTGGGTGTGC TCGACGTGCA GCAAATCTTC GGAAGAGCTG GTCGTCCGCA ATTCGAGAAC 
GAAGGTCACG GAATTATCAT CACAACTCGT GACAAAATCG ACAAATATCT AACAATGCTC 
GTTCACCAAA ACCCGATTGA AAGTCAATTC TACGCTCGGC TTCACGATAA TTTGAACGCT 
GAAGTGGCCC TGGGTACCGT ATCCACAGTG GATGAGGGTG TTGAATGGTT GACCTATACC 
TACATGTACA CGAGAGCCCT GAAAAATCCG ATGGCTTATG GAATTGCGTA TAATGCAATT 
GAACGGGATC CGAATTTGAG AGATCATTTT GGAAATGTGA TTCGCGAGGC GGCGATGCAA 
CTGGATCAGA ATAAAATGAT TCGATTTGAT ATGGCAACCG AATACCTAAA CTCGACGGAT 
CTCGGTCGAA TCGCCTCCAA TTTCTATGTC AAATACGAAA CAATTCAGCT CCTCAACGAA 
GCGGAAAAGG GCGTCGGATT ACCAGTAACT TTCACAAGTT TCATGCCAGA CGACATGGTC 
ATCGGACTCA TCTCAATGTC CACCGAGTTC GCGAATTTGA AGTGTCGTGA GGAGGAGATC 
GGCGATCTGG AAGAGCTGAT GAGCTACGGA TGTATGATGA ACGTTCGTGG TGGTGGTCTG 
GCGAGTGTCG CCGGAAAAGT CAATGTTCTC CTTCAATCAC TGATCTCTAG AACATCGACG 
AGAAACTCGG CGCTCATGTC CGAGCAGCTA TACGTTCAGC AGAACGCCGG TCGGCTCTGC 
AGAGCTATGT TTGAGATGGT TCTGAAGAAC GGGTGGAGCC AGGCGGCCAA TGCCTTTTTG 
GGAATTGCAA AATGTGTCGA GAAGCAGATG TGGATGAATC AGTGCGCGTT GCGGCAGTTT 
ATTCAGATCA TCAACATCCC CATCACATGG ATCGAGAAAA TCGAGCGTAA AAAAGCGAGA 
GAATCGGATT TACTCGAGCT CTCCGCCAAA GATCTCGGCT ACATGTTCAG TTGCGACGGT 
GACCGCCTAT ACACCTACCT ACGCTACCTT CCGAGGATGG ATGTGCAAGC AAAGTTCAAG 
CCGATCACCT ACACGATCGT TCAGGTCGAG GTCACACTCA CGCCGACGTT CATCTGGAAC 
GACCAGATCC ACGGAAAGTC GGGACAACAA TCGTTCTATC TAGTGCTGGA GAATCTCAAC 
GAGAATCTTA TCATCCATCA GGAGAGAATC GGCATCGGGA AGATGAAGGT GAACCGGCAG 
GAGACTCAGA ATCTAGTTTT CACTATTCCA ATCGTCGACT GTCAGCTGAC GAACAATTTC 
CAACTTCGCC TCGCCAGCGA ATACTTTGTC ACCGACGACG TGGTGGTTCC GATGTCTCTT 
CACAATTGCA TTCTACCAAA GTCCTTCAAA AGCCACACAG ATCTTCTGGA CCTTGAGCCA 
CTTCCTATCA AAACTCTCAA AAACTCAAAG TTCGAGGCGA TCTACAACTT TGACTATTTC 
AACCCAATTC AAGCGCAAGT TTTCTTTTGC CTGTACAAAA CCGACAAAAG CGCATTGATC 
GGAGCGCCGA CAGGATCTGG AAAGACACTT TGCGCGGAGC TCGCCATGTT CCGCCTGCTT 
CAGGACCATC CTGGCATGAA AGTCGTGTAC ATTGCTCCGT TGAAGTCCTT GGTCCGAGAG 
CGAGTTGACG ATTGGAAGAA GAAGTTCGAA GATGGAATGG GATATCGAGT GGTCGAGGTG 
TCTGGGGATG TGACTCCAGA TCCCGAGGAG CTTGCGGCTT CCTCAATTCT TATTACGACA 
CCAGAAAAGT GGGATGGAAT CTCCAGAAGT TGGGCTACGA GGGAATACGT CCGACGAGTC 
GGCCTTATCG TGCTCGACGA GGTTCACCTA CTCGGCGTAG ATCGTGGAGC TGTGCTCGAG 
GCCATCGTCA GCAGACTGAA AATGATCACC AGAAGAAGCC ACGTGACAGA GGAGCCTGTC 
AGGTTGCTGG GTCTTTCCAC AGCGCTCGCC AACGCAGGAG ACGTCGCAGA GTGGCTTGGC 
ATCCCCGACG AGGCGTGCTA CAACTTCCGA CCTTCAGTTC GACCAGTGCC GATTTCAGTT 
CATATTCAAG GATTCCCAGG CCAACATTAC TGCCCACGAA TGGGACTGAT GAACAAGCCG 
GCTTACAAGG CAATTCTGAC CTATTCCCCA AGGAAGCCTG TGTTGATCTT CGTCTCGTCT 
AGACGACAAA CCCGCCTGAC AGCGCTCGCC TTTGTCAATT TGCTCATTGC CGACCACAAT 
CCGAAGCAAT GGCTCAACAT GGACATGCTC GAGTTGGAAG ATCTCATGGC CGCAATCAAG 
GATGAAAATC TCAAGTTGAC ACTACCATTC GGAATCGGAA TGCATCACGC TGGACTCAGC 
GCTCACGAGC GAGCCATTGT GGAGCAATTG TTTATCGAAA AGAAGATTCA AGTGCTCATC 
GCGACTGCCA CATTGGCGTG GGGAATCAAT TGCCCCGCTC ATTTAGTTAT TGTCAAGGGA 
ACCGAATATT TCGACGGAAA GAAGGGAAAA TACGTGGATT TTCCCGTTAC AGACGTTCTC 
CAAATGATGG GCCGCGCCGG ACGCCCACAA TTCGACGATT CGGCCGTTGC CGTGATCTAC 
GTACAGGACG CGAAAAAGAC GTTCTATAAG AAGTTCCTCT ACGAACCGTT CCCCGTCGAG 
TCCTCGCTCC TTCCAGTGCT GCCGAATCAC GTGAACGCCG AGATTTCCGC CGGAACAATC 
GATTCGAAGC AGGCGATCGT CGAGTATTTG TCGAAAACCT ATCTCTACAG GCGATTGTTT 
GCGAATCCCA ATTATTACGG TCTGGAGGAG GATTCCGAGG AGGCGATGCT CAAGTTTATC 
ACGAAAATCG TTGATGACTC TGTCGCAGAG CTCCTAGCTT CCGAGTGTAT CCACGTGGAT 
TCCGAACAGG ACGTGATCAA GCCGACGCCG TGTGGAAGGA TAGCTTCGGT GTACTATCTT 
CAGCACGAGA CCGTCCGTTT TCTAGTAAAA TCTCTACATT CTGGATGCTC GGTGGAGAAT 
ATGCTGAAGA TCCTGACCGA TGTTCCCGAG TACGCGGAGA TTCCTGTCCG CCACAACGAG 
GATCTCATCA ATACGGAACT TCAGAAGAAG CTTCGCATCC GATTCTCGAC GTCTGTGATG 
GGCACGTCTG CGTGCAAAGC CCACCTGCTC TTCCAGGCTC ATTTCATGCG GACTGTGCTC 
CCGACGGATT ACCGGACTGA TTTGAAGAGT GTGCTGGATC AGTGCATTCG GATTTTGCAG 
GCGATGCGTG AAATGGCTAG GCTCAAGAAT TGGCTCAGCG CAACGATGAA CATTATTCTG 
TTGCAGCAGA TGTGCCATTC GGCAAGGTGG CACGACGATC ATCCATTGCT CTGCCTACCT 
CATCTCTCCC ACGAGGATGC TCGTTCAATC GGCGATGGAA TGACAATTCC ACAGCTTCAA 
AACCATCTGG AAATCGAGAA ATCCACGTCA TTAGACGACG CGAAACTTGC TCGACGAGCC 
CAGAAACTCT TCAGAGAGTG CACCAAGTTA GATGAAGCTC AAAGCCGGGA AGTGCTGAAA 
GCTCTGTGCA ATTGGCCAAT TATCAACATG AAAATCATGC AATTAGTGGA TTCCCGCGGT 
AATTGTGTAG ATATTGATGA GACGAAGAAA CCGGTGAAAG TGACGGCTGG CGAGGTGTAC 
AAATTGAGAA TTGTCATGGA ACGAGTCGGA CCGGGAAAGA ACAACTCATC AATGCATCTT 
CCACAATGGC CAAAACCAAA ACAGGCTGGA TGGATAATTG TTGTGGGAAA CGTGTCGGCC 
GACATGATTC TTAACACGAC TACCGTAACC GGCTCACATT CGACTCGGTC GACCGCCAAA 
TTGGATATTC GTGCGCCGGC GACGAAAGGA AACCACGAGC TCGCCGTCCT AATTCTCAGC 
GATTGCTATT TGGGAATCGA TCAGGAGTAC ACTTTGCGAT TGGATGTCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_497060.2
CDS	1..6054
Translation	MDLLNYIERIGDDPPSPSRVASFHQEIQIRFPSEFLEWSNCEAEQEGFSEAPEALSLPQVPSTSGSGSIFQPIADTLLIYFPDTFETISQNLLQMLCSTRSDMDLQGELIDLLGVELFELAGDILEKRNKIVFEVKSQELAKVEHARQAKAKPAVPGFLQTVSVQSTSQNEKRRELRKEEKRTRRELNRVIHAFGDEEKLELELAQKEIQRQRQLEIDQMKWKPSLVAGGPRIEEIYPYVFDARLQNVGHTMFDINGMKFALPEGSKRDTFKTHESVYVPPSNKGDIEKIQHVYVKDMDELGQKGFKGFEKLNVIQSIVFEQAYKTKENLLICAPTGAGKTNIAMLTILNTIHEHQNSRGDIMKDDFKIIYIAPMKALATEMTESFGKRLAPLGLKVKELTGDTQLSRNEVADTQMLVLTPEKWDVITRKSTSDNSLINVVRLLIIDEVHLLHDERGPVIETLVARTLRQVEMSQSGIRIVGLSATLPNFIDVARFLRVNPYKGLFFFDGRFRPVPLTQKFIGTRKAGNFRDNLTLMDNVCYDEVVDFVKRGHQVLVFVHTRNGTAKLGEAFCARASVLGQMDLFLPKDRASSKYIQADKAINICRNRAQISPLFQRGMGIHHAGLCRQDRILMERCFAEGHVSVLFCTATLAWGVNLPAHAVVIKGTDVFDAEKGVFTDLGVLDVQQIFGRAGRPQFENEGHGIIITTRDKIDKYLTMLVHQNPIESQFYARLHDNLNAEVALGTVSTVDEGVEWLTYTYMYTRALKNPMAYGIAYNAIERDPNLRDHFGNVIREAAMQLDQNKMIRFDMATEYLNSTDLGRIASNFYVKYETIQLLNEAEKGVGLPVTFTSFMPDDMVIGLISMSTEFANLKCREEEIGDLEELMSYGCMMNVRGGGLASVAGKVNVLLQSLISRTSTRNSALMSEQLYVQQNAGRLCRAMFEMVLKNGWSQAANAFLGIAKCVEKQMWMNQCALRQFIQIINIPITWIEKIERKKARESDLLELSAKDLGYMFSCDGDRLYTYLRYLPRMDVQAKFKPITYTIVQVEVTLTPTFIWNDQIHGKSGQQSFYLVLENLNENLIIHQERIGIGKMKVNRQETQNLVFTIPIVDCQLTNNFQLRLASEYFVTDDVVVPMSLHNCILPKSFKSHTDLLDLEPLPIKTLKNSKFEAIYNFDYFNPIQAQVFFCLYKTDKSALIGAPTGSGKTLCAELAMFRLLQDHPGMKVVYIAPLKSLVRERVDDWKKKFEDGMGYRVVEVSGDVTPDPEELAASSILITTPEKWDGISRSWATREYVRRVGLIVLDEVHLLGVDRGAVLEAIVSRLKMITRRSHVTEEPVRLLGLSTALANAGDVAEWLGIPDEACYNFRPSVRPVPISVHIQGFPGQHYCPRMGLMNKPAYKAILTYSPRKPVLIFVSSRRQTRLTALAFVNLLIADHNPKQWLNMDMLELEDLMAAIKDENLKLTLPFGIGMHHAGLSAHERAIVEQLFIEKKIQVLIATATLAWGINCPAHLVIVKGTEYFDGKKGKYVDFPVTDVLQMMGRAGRPQFDDSAVAVIYVQDAKKTFYKKFLYEPFPVESSLLPVLPNHVNAEISAGTIDSKQAIVEYLSKTYLYRRLFANPNYYGLEEDSEEAMLKFITKIVDDSVAELLASECIHVDSEQDVIKPTPCGRIASVYYLQHETVRFLVKSLHSGCSVENMLKILTDVPEYAEIPVRHNEDLINTELQKKLRIRFSTSVMGTSACKAHLLFQAHFMRTVLPTDYRTDLKSVLDQCIRILQAMREMARLKNWLSATMNIILLQQMCHSARWHDDHPLLCLPHLSHEDARSIGDGMTIPQLQNHLEIEKSTSLDDAKLARRAQKLFRECTKLDEAQSREVLKALCNWPIINMKIMQLVDSRGNCVDIDETKKPVKVTAGEVYKLRIVMERVGPGKNNSSMHLPQWPKPKQAGWIIVVGNVSADMILNTTTVTGSHSTRSTAKLDIRAPATKGNHELAVLILSDCYLGIDQEYTLRLDVC

Target ORF information:

RefSeq Version	NM_064659.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (Y54E2A.4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064659.2

ORF Insert Sequence:

ATGGATCTTC TCAACTATAT CGAACGAATT GGAGATGATC CACCCTCACC ATCACGGGTA 
GCAAGCTTCC ACCAGGAAAT TCAAATTCGA TTTCCATCGG AATTTCTAGA ATGGTCGAAT 
TGTGAAGCTG AACAAGAAGG TTTTTCAGAA GCTCCAGAAG CTCTTTCCCT ACCCCAAGTG 
CCTTCTACCT CGGGCTCTGG GAGCATTTTT CAGCCGATTG CTGACACATT GCTTATATAT 
TTTCCAGATA CGTTTGAGAC AATATCCCAA AACCTTCTAC AAATGTTGTG CTCGACACGT 
TCCGATATGG ATCTACAGGG CGAGCTCATC GATCTTCTCG GCGTCGAATT GTTCGAATTA 
GCCGGAGATA TACTCGAAAA ACGGAATAAA ATAGTTTTCG AGGTGAAGAG CCAGGAGCTG 
GCGAAAGTTG AGCATGCGAG GCAGGCGAAG GCGAAGCCCG CCGTGCCCGG TTTCCTGCAA 
ACTGTCAGCG TACAATCCAC ATCACAAAAC GAGAAACGAC GTGAACTTCG GAAAGAGGAG 
AAGCGAACTC GTCGAGAGCT CAACCGAGTG ATCCATGCGT TCGGCGACGA GGAGAAGCTT 
GAGTTGGAGC TGGCGCAAAA GGAAATCCAA CGCCAACGAC AACTGGAAAT CGATCAAATG 
AAGTGGAAAC CGTCGCTAGT TGCCGGCGGC CCGCGCATCG AGGAAATCTA CCCGTACGTG 
TTCGACGCGA GACTTCAGAA CGTCGGTCAC ACCATGTTCG ACATCAATGG AATGAAGTTC 
GCGCTTCCAG AAGGTTCCAA GCGGGACACC TTTAAGACGC ATGAGAGTGT CTACGTGCCT 
CCGTCGAATA AAGGAGACAT TGAGAAGATT CAGCATGTCT ACGTAAAAGA TATGGATGAG 
CTTGGACAGA AGGGGTTTAA AGGATTTGAA AAACTCAATG TGATTCAATC GATTGTATTC 
GAACAAGCCT ACAAGACCAA GGAGAATCTT CTGATTTGCG CGCCAACCGG AGCCGGAAAG 
ACCAATATTG CGATGCTAAC GATTCTGAAC ACAATCCACG AGCATCAAAA TTCACGAGGA 
GATATTATGA AGGACGACTT TAAAATCATC TACATCGCCC CAATGAAAGC GCTGGCGACC 
GAGATGACCG AGTCATTCGG TAAACGGTTA GCACCGCTTG GCTTGAAAGT GAAGGAGCTC 
ACCGGAGACA CACAACTCTC GAGAAATGAG GTAGCGGATA CTCAGATGTT GGTGCTGACG 
CCTGAGAAAT GGGATGTGAT CACGAGAAAA TCGACCAGTG ACAACTCGCT CATAAATGTG 
GTCCGGCTGC TGATCATCGA CGAAGTTCAT CTGCTTCACG ATGAACGTGG TCCGGTTATT 
GAGACCCTGG TCGCCAGAAC GCTCCGACAG GTCGAGATGT CACAGTCCGG GATTCGAATT 
GTCGGATTGT CGGCGACGCT GCCGAATTTC ATCGATGTCG CCAGATTCTT ACGGGTCAAT 
CCCTACAAGG GCTTGTTCTT CTTCGATGGA AGGTTCAGGC CGGTGCCATT GACTCAGAAA 
TTCATCGGAA CACGAAAAGC GGGCAACTTC CGTGACAATC TCACTCTAAT GGACAACGTG 
TGCTACGACG AAGTGGTGGA TTTTGTGAAA CGCGGCCATC AGGTCCTTGT CTTCGTGCAC 
ACGCGAAACG GAACCGCAAA GCTCGGAGAA GCGTTCTGTG CTCGAGCTTC AGTCCTCGGA 
CAGATGGACC TATTCCTGCC GAAAGATCGA GCGTCGAGCA AGTACATACA AGCGGACAAG 
GCGATCAATA TTTGCCGGAA TCGGGCTCAA ATCTCGCCAC TTTTCCAACG TGGAATGGGA 
ATTCATCACG CTGGACTCTG TCGACAGGAT CGTATTCTCA TGGAGCGATG CTTTGCCGAG 
GGGCATGTTT CTGTGCTGTT CTGTACGGCA ACTCTGGCGT GGGGAGTCAA TTTGCCGGCT 
CATGCGGTTG TTATCAAGGG AACCGATGTT TTTGATGCGG AGAAGGGTGT TTTTACGGAT 
TTGGGTGTGC TCGACGTGCA GCAAATCTTC GGAAGAGCTG GTCGTCCGCA ATTCGAGAAC 
GAAGGTCACG GAATTATCAT CACAACTCGT GACAAAATCG ACAAATATCT AACAATGCTC 
GTTCACCAAA ACCCGATTGA AAGTCAATTC TACGCTCGGC TTCACGATAA TTTGAACGCT 
GAAGTGGCCC TGGGTACCGT ATCCACAGTG GATGAGGGTG TTGAATGGTT GACCTATACC 
TACATGTACA CGAGAGCCCT GAAAAATCCG ATGGCTTATG GAATTGCGTA TAATGCAATT 
GAACGGGATC CGAATTTGAG AGATCATTTT GGAAATGTGA TTCGCGAGGC GGCGATGCAA 
CTGGATCAGA ATAAAATGAT TCGATTTGAT ATGGCAACCG AATACCTAAA CTCGACGGAT 
CTCGGTCGAA TCGCCTCCAA TTTCTATGTC AAATACGAAA CAATTCAGCT CCTCAACGAA 
GCGGAAAAGG GCGTCGGATT ACCAGTAACT TTCACAAGTT TCATGCCAGA CGACATGGTC 
ATCGGACTCA TCTCAATGTC CACCGAGTTC GCGAATTTGA AGTGTCGTGA GGAGGAGATC 
GGCGATCTGG AAGAGCTGAT GAGCTACGGA TGTATGATGA ACGTTCGTGG TGGTGGTCTG 
GCGAGTGTCG CCGGAAAAGT CAATGTTCTC CTTCAATCAC TGATCTCTAG AACATCGACG 
AGAAACTCGG CGCTCATGTC CGAGCAGCTA TACGTTCAGC AGAACGCCGG TCGGCTCTGC 
AGAGCTATGT TTGAGATGGT TCTGAAGAAC GGGTGGAGCC AGGCGGCCAA TGCCTTTTTG 
GGAATTGCAA AATGTGTCGA GAAGCAGATG TGGATGAATC AGTGCGCGTT GCGGCAGTTT 
ATTCAGATCA TCAACATCCC CATCACATGG ATCGAGAAAA TCGAGCGTAA AAAAGCGAGA 
GAATCGGATT TACTCGAGCT CTCCGCCAAA GATCTCGGCT ACATGTTCAG TTGCGACGGT 
GACCGCCTAT ACACCTACCT ACGCTACCTT CCGAGGATGG ATGTGCAAGC AAAGTTCAAG 
CCGATCACCT ACACGATCGT TCAGGTCGAG GTCACACTCA CGCCGACGTT CATCTGGAAC 
GACCAGATCC ACGGAAAGTC GGGACAACAA TCGTTCTATC TAGTGCTGGA GAATCTCAAC 
GAGAATCTTA TCATCCATCA GGAGAGAATC GGCATCGGGA AGATGAAGGT GAACCGGCAG 
GAGACTCAGA ATCTAGTTTT CACTATTCCA ATCGTCGACT GTCAGCTGAC GAACAATTTC 
CAACTTCGCC TCGCCAGCGA ATACTTTGTC ACCGACGACG TGGTGGTTCC GATGTCTCTT 
CACAATTGCA TTCTACCAAA GTCCTTCAAA AGCCACACAG ATCTTCTGGA CCTTGAGCCA 
CTTCCTATCA AAACTCTCAA AAACTCAAAG TTCGAGGCGA TCTACAACTT TGACTATTTC 
AACCCAATTC AAGCGCAAGT TTTCTTTTGC CTGTACAAAA CCGACAAAAG CGCATTGATC 
GGAGCGCCGA CAGGATCTGG AAAGACACTT TGCGCGGAGC TCGCCATGTT CCGCCTGCTT 
CAGGACCATC CTGGCATGAA AGTCGTGTAC ATTGCTCCGT TGAAGTCCTT GGTCCGAGAG 
CGAGTTGACG ATTGGAAGAA GAAGTTCGAA GATGGAATGG GATATCGAGT GGTCGAGGTG 
TCTGGGGATG TGACTCCAGA TCCCGAGGAG CTTGCGGCTT CCTCAATTCT TATTACGACA 
CCAGAAAAGT GGGATGGAAT CTCCAGAAGT TGGGCTACGA GGGAATACGT CCGACGAGTC 
GGCCTTATCG TGCTCGACGA GGTTCACCTA CTCGGCGTAG ATCGTGGAGC TGTGCTCGAG 
GCCATCGTCA GCAGACTGAA AATGATCACC AGAAGAAGCC ACGTGACAGA GGAGCCTGTC 
AGGTTGCTGG GTCTTTCCAC AGCGCTCGCC AACGCAGGAG ACGTCGCAGA GTGGCTTGGC 
ATCCCCGACG AGGCGTGCTA CAACTTCCGA CCTTCAGTTC GACCAGTGCC GATTTCAGTT 
CATATTCAAG GATTCCCAGG CCAACATTAC TGCCCACGAA TGGGACTGAT GAACAAGCCG 
GCTTACAAGG CAATTCTGAC CTATTCCCCA AGGAAGCCTG TGTTGATCTT CGTCTCGTCT 
AGACGACAAA CCCGCCTGAC AGCGCTCGCC TTTGTCAATT TGCTCATTGC CGACCACAAT 
CCGAAGCAAT GGCTCAACAT GGACATGCTC GAGTTGGAAG ATCTCATGGC CGCAATCAAG 
GATGAAAATC TCAAGTTGAC ACTACCATTC GGAATCGGAA TGCATCACGC TGGACTCAGC 
GCTCACGAGC GAGCCATTGT GGAGCAATTG TTTATCGAAA AGAAGATTCA AGTGCTCATC 
GCGACTGCCA CATTGGCGTG GGGAATCAAT TGCCCCGCTC ATTTAGTTAT TGTCAAGGGA 
ACCGAATATT TCGACGGAAA GAAGGGAAAA TACGTGGATT TTCCCGTTAC AGACGTTCTC 
CAAATGATGG GCCGCGCCGG ACGCCCACAA TTCGACGATT CGGCCGTTGC CGTGATCTAC 
GTACAGGACG CGAAAAAGAC GTTCTATAAG AAGTTCCTCT ACGAACCGTT CCCCGTCGAG 
TCCTCGCTCC TTCCAGTGCT GCCGAATCAC GTGAACGCCG AGATTTCCGC CGGAACAATC 
GATTCGAAGC AGGCGATCGT CGAGTATTTG TCGAAAACCT ATCTCTACAG GCGATTGTTT 
GCGAATCCCA ATTATTACGG TCTGGAGGAG GATTCCGAGG AGGCGATGCT CAAGTTTATC 
ACGAAAATCG TTGATGACTC TGTCGCAGAG CTCCTAGCTT CCGAGTGTAT CCACGTGGAT 
TCCGAACAGG ACGTGATCAA GCCGACGCCG TGTGGAAGGA TAGCTTCGGT GTACTATCTT 
CAGCACGAGA CCGTCCGTTT TCTAGTAAAA TCTCTACATT CTGGATGCTC GGTGGAGAAT 
ATGCTGAAGA TCCTGACCGA TGTTCCCGAG TACGCGGAGA TTCCTGTCCG CCACAACGAG 
GATCTCATCA ATACGGAACT TCAGAAGAAG CTTCGCATCC GATTCTCGAC GTCTGTGATG 
GGCACGTCTG CGTGCAAAGC CCACCTGCTC TTCCAGGCTC ATTTCATGCG GACTGTGCTC 
CCGACGGATT ACCGGACTGA TTTGAAGAGT GTGCTGGATC AGTGCATTCG GATTTTGCAG 
GCGATGCGTG AAATGGCTAG GCTCAAGAAT TGGCTCAGCG CAACGATGAA CATTATTCTG 
TTGCAGCAGA TGTGCCATTC GGCAAGGTGG CACGACGATC ATCCATTGCT CTGCCTACCT 
CATCTCTCCC ACGAGGATGC TCGTTCAATC GGCGATGGAA TGACAATTCC ACAGCTTCAA 
AACCATCTGG AAATCGAGAA ATCCACGTCA TTAGACGACG CGAAACTTGC TCGACGAGCC 
CAGAAACTCT TCAGAGAGTG CACCAAGTTA GATGAAGCTC AAAGCCGGGA AGTGCTGAAA 
GCTCTGTGCA ATTGGCCAAT TATCAACATG AAAATCATGC AATTAGTGGA TTCCCGCGGT 
AATTGTGTAG ATATTGATGA GACGAAGAAA CCGGTGAAAG TGACGGCTGG CGAGGTGTAC 
AAATTGAGAA TTGTCATGGA ACGAGTCGGA CCGGGAAAGA ACAACTCATC AATGCATCTT 
CCACAATGGC CAAAACCAAA ACAGGCTGGA TGGATAATTG TTGTGGGAAA CGTGTCGGCC 
GACATGATTC TTAACACGAC TACCGTAACC GGCTCACATT CGACTCGGTC GACCGCCAAA 
TTGGATATTC GTGCGCCGGC GACGAAAGGA AACCACGAGC TCGCCGTCCT AATTCTCAGC 
GATTGCTATT TGGGAATCGA TCAGGAGTAC ACTTTGCGAT TGGATGTCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Y54E2A.4 ( NM_064659.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_497060.2 Caenorhabditis elegans SEC63 domain-containing protein (Y54E2A.4), mRNA.

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CloneID	OCa360030
Clone ID Related Accession (Same CDS sequence)	NM_064659.2 , NM_064659.3
Accession Version	NM_064659.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SEC63 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_064659.2.

ATGGATCTTC TCAACTATAT CGAACGAATT GGAGATGATC CACCCTCACC ATCACGGGTA 
GCAAGCTTCC ACCAGGAAAT TCAAATTCGA TTTCCATCGG AATTTCTAGA ATGGTCGAAT 
TGTGAAGCTG AACAAGAAGG TTTTTCAGAA GCTCCAGAAG CTCTTTCCCT ACCCCAAGTG 
CCTTCTACCT CGGGCTCTGG GAGCATTTTT CAGCCGATTG CTGACACATT GCTTATATAT 
TTTCCAGATA CGTTTGAGAC AATATCCCAA AACCTTCTAC AAATGTTGTG CTCGACACGT 
TCCGATATGG ATCTACAGGG CGAGCTCATC GATCTTCTCG GCGTCGAATT GTTCGAATTA 
GCCGGAGATA TACTCGAAAA ACGGAATAAA ATAGTTTTCG AGGTGAAGAG CCAGGAGCTG 
GCGAAAGTTG AGCATGCGAG GCAGGCGAAG GCGAAGCCCG CCGTGCCCGG TTTCCTGCAA 
ACTGTCAGCG TACAATCCAC ATCACAAAAC GAGAAACGAC GTGAACTTCG GAAAGAGGAG 
AAGCGAACTC GTCGAGAGCT CAACCGAGTG ATCCATGCGT TCGGCGACGA GGAGAAGCTT 
GAGTTGGAGC TGGCGCAAAA GGAAATCCAA CGCCAACGAC AACTGGAAAT CGATCAAATG 
AAGTGGAAAC CGTCGCTAGT TGCCGGCGGC CCGCGCATCG AGGAAATCTA CCCGTACGTG 
TTCGACGCGA GACTTCAGAA CGTCGGTCAC ACCATGTTCG ACATCAATGG AATGAAGTTC 
GCGCTTCCAG AAGGTTCCAA GCGGGACACC TTTAAGACGC ATGAGAGTGT CTACGTGCCT 
CCGTCGAATA AAGGAGACAT TGAGAAGATT CAGCATGTCT ACGTAAAAGA TATGGATGAG 
CTTGGACAGA AGGGGTTTAA AGGATTTGAA AAACTCAATG TGATTCAATC GATTGTATTC 
GAACAAGCCT ACAAGACCAA GGAGAATCTT CTGATTTGCG CGCCAACCGG AGCCGGAAAG 
ACCAATATTG CGATGCTAAC GATTCTGAAC ACAATCCACG AGCATCAAAA TTCACGAGGA 
GATATTATGA AGGACGACTT TAAAATCATC TACATCGCCC CAATGAAAGC GCTGGCGACC 
GAGATGACCG AGTCATTCGG TAAACGGTTA GCACCGCTTG GCTTGAAAGT GAAGGAGCTC 
ACCGGAGACA CACAACTCTC GAGAAATGAG GTAGCGGATA CTCAGATGTT GGTGCTGACG 
CCTGAGAAAT GGGATGTGAT CACGAGAAAA TCGACCAGTG ACAACTCGCT CATAAATGTG 
GTCCGGCTGC TGATCATCGA CGAAGTTCAT CTGCTTCACG ATGAACGTGG TCCGGTTATT 
GAGACCCTGG TCGCCAGAAC GCTCCGACAG GTCGAGATGT CACAGTCCGG GATTCGAATT 
GTCGGATTGT CGGCGACGCT GCCGAATTTC ATCGATGTCG CCAGATTCTT ACGGGTCAAT 
CCCTACAAGG GCTTGTTCTT CTTCGATGGA AGGTTCAGGC CGGTGCCATT GACTCAGAAA 
TTCATCGGAA CACGAAAAGC GGGCAACTTC CGTGACAATC TCACTCTAAT GGACAACGTG 
TGCTACGACG AAGTGGTGGA TTTTGTGAAA CGCGGCCATC AGGTCCTTGT CTTCGTGCAC 
ACGCGAAACG GAACCGCAAA GCTCGGAGAA GCGTTCTGTG CTCGAGCTTC AGTCCTCGGA 
CAGATGGACC TATTCCTGCC GAAAGATCGA GCGTCGAGCA AGTACATACA AGCGGACAAG 
GCGATCAATA TTTGCCGGAA TCGGGCTCAA ATCTCGCCAC TTTTCCAACG TGGAATGGGA 
ATTCATCACG CTGGACTCTG TCGACAGGAT CGTATTCTCA TGGAGCGATG CTTTGCCGAG 
GGGCATGTTT CTGTGCTGTT CTGTACGGCA ACTCTGGCGT GGGGAGTCAA TTTGCCGGCT 
CATGCGGTTG TTATCAAGGG AACCGATGTT TTTGATGCGG AGAAGGGTGT TTTTACGGAT 
TTGGGTGTGC TCGACGTGCA GCAAATCTTC GGAAGAGCTG GTCGTCCGCA ATTCGAGAAC 
GAAGGTCACG GAATTATCAT CACAACTCGT GACAAAATCG ACAAATATCT AACAATGCTC 
GTTCACCAAA ACCCGATTGA AAGTCAATTC TACGCTCGGC TTCACGATAA TTTGAACGCT 
GAAGTGGCCC TGGGTACCGT ATCCACAGTG GATGAGGGTG TTGAATGGTT GACCTATACC 
TACATGTACA CGAGAGCCCT GAAAAATCCG ATGGCTTATG GAATTGCGTA TAATGCAATT 
GAACGGGATC CGAATTTGAG AGATCATTTT GGAAATGTGA TTCGCGAGGC GGCGATGCAA 
CTGGATCAGA ATAAAATGAT TCGATTTGAT ATGGCAACCG AATACCTAAA CTCGACGGAT 
CTCGGTCGAA TCGCCTCCAA TTTCTATGTC AAATACGAAA CAATTCAGCT CCTCAACGAA 
GCGGAAAAGG GCGTCGGATT ACCAGTAACT TTCACAAGTT TCATGCCAGA CGACATGGTC 
ATCGGACTCA TCTCAATGTC CACCGAGTTC GCGAATTTGA AGTGTCGTGA GGAGGAGATC 
GGCGATCTGG AAGAGCTGAT GAGCTACGGA TGTATGATGA ACGTTCGTGG TGGTGGTCTG 
GCGAGTGTCG CCGGAAAAGT CAATGTTCTC CTTCAATCAC TGATCTCTAG AACATCGACG 
AGAAACTCGG CGCTCATGTC CGAGCAGCTA TACGTTCAGC AGAACGCCGG TCGGCTCTGC 
AGAGCTATGT TTGAGATGGT TCTGAAGAAC GGGTGGAGCC AGGCGGCCAA TGCCTTTTTG 
GGAATTGCAA AATGTGTCGA GAAGCAGATG TGGATGAATC AGTGCGCGTT GCGGCAGTTT 
ATTCAGATCA TCAACATCCC CATCACATGG ATCGAGAAAA TCGAGCGTAA AAAAGCGAGA 
GAATCGGATT TACTCGAGCT CTCCGCCAAA GATCTCGGCT ACATGTTCAG TTGCGACGGT 
GACCGCCTAT ACACCTACCT ACGCTACCTT CCGAGGATGG ATGTGCAAGC AAAGTTCAAG 
CCGATCACCT ACACGATCGT TCAGGTCGAG GTCACACTCA CGCCGACGTT CATCTGGAAC 
GACCAGATCC ACGGAAAGTC GGGACAACAA TCGTTCTATC TAGTGCTGGA GAATCTCAAC 
GAGAATCTTA TCATCCATCA GGAGAGAATC GGCATCGGGA AGATGAAGGT GAACCGGCAG 
GAGACTCAGA ATCTAGTTTT CACTATTCCA ATCGTCGACT GTCAGCTGAC GAACAATTTC 
CAACTTCGCC TCGCCAGCGA ATACTTTGTC ACCGACGACG TGGTGGTTCC GATGTCTCTT 
CACAATTGCA TTCTACCAAA GTCCTTCAAA AGCCACACAG ATCTTCTGGA CCTTGAGCCA 
CTTCCTATCA AAACTCTCAA AAACTCAAAG TTCGAGGCGA TCTACAACTT TGACTATTTC 
AACCCAATTC AAGCGCAAGT TTTCTTTTGC CTGTACAAAA CCGACAAAAG CGCATTGATC 
GGAGCGCCGA CAGGATCTGG AAAGACACTT TGCGCGGAGC TCGCCATGTT CCGCCTGCTT 
CAGGACCATC CTGGCATGAA AGTCGTGTAC ATTGCTCCGT TGAAGTCCTT GGTCCGAGAG 
CGAGTTGACG ATTGGAAGAA GAAGTTCGAA GATGGAATGG GATATCGAGT GGTCGAGGTG 
TCTGGGGATG TGACTCCAGA TCCCGAGGAG CTTGCGGCTT CCTCAATTCT TATTACGACA 
CCAGAAAAGT GGGATGGAAT CTCCAGAAGT TGGGCTACGA GGGAATACGT CCGACGAGTC 
GGCCTTATCG TGCTCGACGA GGTTCACCTA CTCGGCGTAG ATCGTGGAGC TGTGCTCGAG 
GCCATCGTCA GCAGACTGAA AATGATCACC AGAAGAAGCC ACGTGACAGA GGAGCCTGTC 
AGGTTGCTGG GTCTTTCCAC AGCGCTCGCC AACGCAGGAG ACGTCGCAGA GTGGCTTGGC 
ATCCCCGACG AGGCGTGCTA CAACTTCCGA CCTTCAGTTC GACCAGTGCC GATTTCAGTT 
CATATTCAAG GATTCCCAGG CCAACATTAC TGCCCACGAA TGGGACTGAT GAACAAGCCG 
GCTTACAAGG CAATTCTGAC CTATTCCCCA AGGAAGCCTG TGTTGATCTT CGTCTCGTCT 
AGACGACAAA CCCGCCTGAC AGCGCTCGCC TTTGTCAATT TGCTCATTGC CGACCACAAT 
CCGAAGCAAT GGCTCAACAT GGACATGCTC GAGTTGGAAG ATCTCATGGC CGCAATCAAG 
GATGAAAATC TCAAGTTGAC ACTACCATTC GGAATCGGAA TGCATCACGC TGGACTCAGC 
GCTCACGAGC GAGCCATTGT GGAGCAATTG TTTATCGAAA AGAAGATTCA AGTGCTCATC 
GCGACTGCCA CATTGGCGTG GGGAATCAAT TGCCCCGCTC ATTTAGTTAT TGTCAAGGGA 
ACCGAATATT TCGACGGAAA GAAGGGAAAA TACGTGGATT TTCCCGTTAC AGACGTTCTC 
CAAATGATGG GCCGCGCCGG ACGCCCACAA TTCGACGATT CGGCCGTTGC CGTGATCTAC 
GTACAGGACG CGAAAAAGAC GTTCTATAAG AAGTTCCTCT ACGAACCGTT CCCCGTCGAG 
TCCTCGCTCC TTCCAGTGCT GCCGAATCAC GTGAACGCCG AGATTTCCGC CGGAACAATC 
GATTCGAAGC AGGCGATCGT CGAGTATTTG TCGAAAACCT ATCTCTACAG GCGATTGTTT 
GCGAATCCCA ATTATTACGG TCTGGAGGAG GATTCCGAGG AGGCGATGCT CAAGTTTATC 
ACGAAAATCG TTGATGACTC TGTCGCAGAG CTCCTAGCTT CCGAGTGTAT CCACGTGGAT 
TCCGAACAGG ACGTGATCAA GCCGACGCCG TGTGGAAGGA TAGCTTCGGT GTACTATCTT 
CAGCACGAGA CCGTCCGTTT TCTAGTAAAA TCTCTACATT CTGGATGCTC GGTGGAGAAT 
ATGCTGAAGA TCCTGACCGA TGTTCCCGAG TACGCGGAGA TTCCTGTCCG CCACAACGAG 
GATCTCATCA ATACGGAACT TCAGAAGAAG CTTCGCATCC GATTCTCGAC GTCTGTGATG 
GGCACGTCTG CGTGCAAAGC CCACCTGCTC TTCCAGGCTC ATTTCATGCG GACTGTGCTC 
CCGACGGATT ACCGGACTGA TTTGAAGAGT GTGCTGGATC AGTGCATTCG GATTTTGCAG 
GCGATGCGTG AAATGGCTAG GCTCAAGAAT TGGCTCAGCG CAACGATGAA CATTATTCTG 
TTGCAGCAGA TGTGCCATTC GGCAAGGTGG CACGACGATC ATCCATTGCT CTGCCTACCT 
CATCTCTCCC ACGAGGATGC TCGTTCAATC GGCGATGGAA TGACAATTCC ACAGCTTCAA 
AACCATCTGG AAATCGAGAA ATCCACGTCA TTAGACGACG CGAAACTTGC TCGACGAGCC 
CAGAAACTCT TCAGAGAGTG CACCAAGTTA GATGAAGCTC AAAGCCGGGA AGTGCTGAAA 
GCTCTGTGCA ATTGGCCAAT TATCAACATG AAAATCATGC AATTAGTGGA TTCCCGCGGT 
AATTGTGTAG ATATTGATGA GACGAAGAAA CCGGTGAAAG TGACGGCTGG CGAGGTGTAC 
AAATTGAGAA TTGTCATGGA ACGAGTCGGA CCGGGAAAGA ACAACTCATC AATGCATCTT 
CCACAATGGC CAAAACCAAA ACAGGCTGGA TGGATAATTG TTGTGGGAAA CGTGTCGGCC 
GACATGATTC TTAACACGAC TACCGTAACC GGCTCACATT CGACTCGGTC GACCGCCAAA 
TTGGATATTC GTGCGCCGGC GACGAAAGGA AACCACGAGC TCGCCGTCCT AATTCTCAGC 
GATTGCTATT TGGGAATCGA TCAGGAGTAC ACTTTGCGAT TGGATGTCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_497060.2
CDS	48..6101
Translation	MDLLNYIERIGDDPPSPSRVASFHQEIQIRFPSEFLEWSNCEAEQEGFSEAPEALSLPQVPSTSGSGSIFQPIADTLLIYFPDTFETISQNLLQMLCSTRSDMDLQGELIDLLGVELFELAGDILEKRNKIVFEVKSQELAKVEHARQAKAKPAVPGFLQTVSVQSTSQNEKRRELRKEEKRTRRELNRVIHAFGDEEKLELELAQKEIQRQRQLEIDQMKWKPSLVAGGPRIEEIYPYVFDARLQNVGHTMFDINGMKFALPEGSKRDTFKTHESVYVPPSNKGDIEKIQHVYVKDMDELGQKGFKGFEKLNVIQSIVFEQAYKTKENLLICAPTGAGKTNIAMLTILNTIHEHQNSRGDIMKDDFKIIYIAPMKALATEMTESFGKRLAPLGLKVKELTGDTQLSRNEVADTQMLVLTPEKWDVITRKSTSDNSLINVVRLLIIDEVHLLHDERGPVIETLVARTLRQVEMSQSGIRIVGLSATLPNFIDVARFLRVNPYKGLFFFDGRFRPVPLTQKFIGTRKAGNFRDNLTLMDNVCYDEVVDFVKRGHQVLVFVHTRNGTAKLGEAFCARASVLGQMDLFLPKDRASSKYIQADKAINICRNRAQISPLFQRGMGIHHAGLCRQDRILMERCFAEGHVSVLFCTATLAWGVNLPAHAVVIKGTDVFDAEKGVFTDLGVLDVQQIFGRAGRPQFENEGHGIIITTRDKIDKYLTMLVHQNPIESQFYARLHDNLNAEVALGTVSTVDEGVEWLTYTYMYTRALKNPMAYGIAYNAIERDPNLRDHFGNVIREAAMQLDQNKMIRFDMATEYLNSTDLGRIASNFYVKYETIQLLNEAEKGVGLPVTFTSFMPDDMVIGLISMSTEFANLKCREEEIGDLEELMSYGCMMNVRGGGLASVAGKVNVLLQSLISRTSTRNSALMSEQLYVQQNAGRLCRAMFEMVLKNGWSQAANAFLGIAKCVEKQMWMNQCALRQFIQIINIPITWIEKIERKKARESDLLELSAKDLGYMFSCDGDRLYTYLRYLPRMDVQAKFKPITYTIVQVEVTLTPTFIWNDQIHGKSGQQSFYLVLENLNENLIIHQERIGIGKMKVNRQETQNLVFTIPIVDCQLTNNFQLRLASEYFVTDDVVVPMSLHNCILPKSFKSHTDLLDLEPLPIKTLKNSKFEAIYNFDYFNPIQAQVFFCLYKTDKSALIGAPTGSGKTLCAELAMFRLLQDHPGMKVVYIAPLKSLVRERVDDWKKKFEDGMGYRVVEVSGDVTPDPEELAASSILITTPEKWDGISRSWATREYVRRVGLIVLDEVHLLGVDRGAVLEAIVSRLKMITRRSHVTEEPVRLLGLSTALANAGDVAEWLGIPDEACYNFRPSVRPVPISVHIQGFPGQHYCPRMGLMNKPAYKAILTYSPRKPVLIFVSSRRQTRLTALAFVNLLIADHNPKQWLNMDMLELEDLMAAIKDENLKLTLPFGIGMHHAGLSAHERAIVEQLFIEKKIQVLIATATLAWGINCPAHLVIVKGTEYFDGKKGKYVDFPVTDVLQMMGRAGRPQFDDSAVAVIYVQDAKKTFYKKFLYEPFPVESSLLPVLPNHVNAEISAGTIDSKQAIVEYLSKTYLYRRLFANPNYYGLEEDSEEAMLKFITKIVDDSVAELLASECIHVDSEQDVIKPTPCGRIASVYYLQHETVRFLVKSLHSGCSVENMLKILTDVPEYAEIPVRHNEDLINTELQKKLRIRFSTSVMGTSACKAHLLFQAHFMRTVLPTDYRTDLKSVLDQCIRILQAMREMARLKNWLSATMNIILLQQMCHSARWHDDHPLLCLPHLSHEDARSIGDGMTIPQLQNHLEIEKSTSLDDAKLARRAQKLFRECTKLDEAQSREVLKALCNWPIINMKIMQLVDSRGNCVDIDETKKPVKVTAGEVYKLRIVMERVGPGKNNSSMHLPQWPKPKQAGWIIVVGNVSADMILNTTTVTGSHSTRSTAKLDIRAPATKGNHELAVLILSDCYLGIDQEYTLRLDVC

Target ORF information:

RefSeq Version	NM_064659.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SEC63 domain-containing protein (Y54E2A.4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064659.3

ORF Insert Sequence:

ATGGATCTTC TCAACTATAT CGAACGAATT GGAGATGATC CACCCTCACC ATCACGGGTA 
GCAAGCTTCC ACCAGGAAAT TCAAATTCGA TTTCCATCGG AATTTCTAGA ATGGTCGAAT 
TGTGAAGCTG AACAAGAAGG TTTTTCAGAA GCTCCAGAAG CTCTTTCCCT ACCCCAAGTG 
CCTTCTACCT CGGGCTCTGG GAGCATTTTT CAGCCGATTG CTGACACATT GCTTATATAT 
TTTCCAGATA CGTTTGAGAC AATATCCCAA AACCTTCTAC AAATGTTGTG CTCGACACGT 
TCCGATATGG ATCTACAGGG CGAGCTCATC GATCTTCTCG GCGTCGAATT GTTCGAATTA 
GCCGGAGATA TACTCGAAAA ACGGAATAAA ATAGTTTTCG AGGTGAAGAG CCAGGAGCTG 
GCGAAAGTTG AGCATGCGAG GCAGGCGAAG GCGAAGCCCG CCGTGCCCGG TTTCCTGCAA 
ACTGTCAGCG TACAATCCAC ATCACAAAAC GAGAAACGAC GTGAACTTCG GAAAGAGGAG 
AAGCGAACTC GTCGAGAGCT CAACCGAGTG ATCCATGCGT TCGGCGACGA GGAGAAGCTT 
GAGTTGGAGC TGGCGCAAAA GGAAATCCAA CGCCAACGAC AACTGGAAAT CGATCAAATG 
AAGTGGAAAC CGTCGCTAGT TGCCGGCGGC CCGCGCATCG AGGAAATCTA CCCGTACGTG 
TTCGACGCGA GACTTCAGAA CGTCGGTCAC ACCATGTTCG ACATCAATGG AATGAAGTTC 
GCGCTTCCAG AAGGTTCCAA GCGGGACACC TTTAAGACGC ATGAGAGTGT CTACGTGCCT 
CCGTCGAATA AAGGAGACAT TGAGAAGATT CAGCATGTCT ACGTAAAAGA TATGGATGAG 
CTTGGACAGA AGGGGTTTAA AGGATTTGAA AAACTCAATG TGATTCAATC GATTGTATTC 
GAACAAGCCT ACAAGACCAA GGAGAATCTT CTGATTTGCG CGCCAACCGG AGCCGGAAAG 
ACCAATATTG CGATGCTAAC GATTCTGAAC ACAATCCACG AGCATCAAAA TTCACGAGGA 
GATATTATGA AGGACGACTT TAAAATCATC TACATCGCCC CAATGAAAGC GCTGGCGACC 
GAGATGACCG AGTCATTCGG TAAACGGTTA GCACCGCTTG GCTTGAAAGT GAAGGAGCTC 
ACCGGAGACA CACAACTCTC GAGAAATGAG GTAGCGGATA CTCAGATGTT GGTGCTGACG 
CCTGAGAAAT GGGATGTGAT CACGAGAAAA TCGACCAGTG ACAACTCGCT CATAAATGTG 
GTCCGGCTGC TGATCATCGA CGAAGTTCAT CTGCTTCACG ATGAACGTGG TCCGGTTATT 
GAGACCCTGG TCGCCAGAAC GCTCCGACAG GTCGAGATGT CACAGTCCGG GATTCGAATT 
GTCGGATTGT CGGCGACGCT GCCGAATTTC ATCGATGTCG CCAGATTCTT ACGGGTCAAT 
CCCTACAAGG GCTTGTTCTT CTTCGATGGA AGGTTCAGGC CGGTGCCATT GACTCAGAAA 
TTCATCGGAA CACGAAAAGC GGGCAACTTC CGTGACAATC TCACTCTAAT GGACAACGTG 
TGCTACGACG AAGTGGTGGA TTTTGTGAAA CGCGGCCATC AGGTCCTTGT CTTCGTGCAC 
ACGCGAAACG GAACCGCAAA GCTCGGAGAA GCGTTCTGTG CTCGAGCTTC AGTCCTCGGA 
CAGATGGACC TATTCCTGCC GAAAGATCGA GCGTCGAGCA AGTACATACA AGCGGACAAG 
GCGATCAATA TTTGCCGGAA TCGGGCTCAA ATCTCGCCAC TTTTCCAACG TGGAATGGGA 
ATTCATCACG CTGGACTCTG TCGACAGGAT CGTATTCTCA TGGAGCGATG CTTTGCCGAG 
GGGCATGTTT CTGTGCTGTT CTGTACGGCA ACTCTGGCGT GGGGAGTCAA TTTGCCGGCT 
CATGCGGTTG TTATCAAGGG AACCGATGTT TTTGATGCGG AGAAGGGTGT TTTTACGGAT 
TTGGGTGTGC TCGACGTGCA GCAAATCTTC GGAAGAGCTG GTCGTCCGCA ATTCGAGAAC 
GAAGGTCACG GAATTATCAT CACAACTCGT GACAAAATCG ACAAATATCT AACAATGCTC 
GTTCACCAAA ACCCGATTGA AAGTCAATTC TACGCTCGGC TTCACGATAA TTTGAACGCT 
GAAGTGGCCC TGGGTACCGT ATCCACAGTG GATGAGGGTG TTGAATGGTT GACCTATACC 
TACATGTACA CGAGAGCCCT GAAAAATCCG ATGGCTTATG GAATTGCGTA TAATGCAATT 
GAACGGGATC CGAATTTGAG AGATCATTTT GGAAATGTGA TTCGCGAGGC GGCGATGCAA 
CTGGATCAGA ATAAAATGAT TCGATTTGAT ATGGCAACCG AATACCTAAA CTCGACGGAT 
CTCGGTCGAA TCGCCTCCAA TTTCTATGTC AAATACGAAA CAATTCAGCT CCTCAACGAA 
GCGGAAAAGG GCGTCGGATT ACCAGTAACT TTCACAAGTT TCATGCCAGA CGACATGGTC 
ATCGGACTCA TCTCAATGTC CACCGAGTTC GCGAATTTGA AGTGTCGTGA GGAGGAGATC 
GGCGATCTGG AAGAGCTGAT GAGCTACGGA TGTATGATGA ACGTTCGTGG TGGTGGTCTG 
GCGAGTGTCG CCGGAAAAGT CAATGTTCTC CTTCAATCAC TGATCTCTAG AACATCGACG 
AGAAACTCGG CGCTCATGTC CGAGCAGCTA TACGTTCAGC AGAACGCCGG TCGGCTCTGC 
AGAGCTATGT TTGAGATGGT TCTGAAGAAC GGGTGGAGCC AGGCGGCCAA TGCCTTTTTG 
GGAATTGCAA AATGTGTCGA GAAGCAGATG TGGATGAATC AGTGCGCGTT GCGGCAGTTT 
ATTCAGATCA TCAACATCCC CATCACATGG ATCGAGAAAA TCGAGCGTAA AAAAGCGAGA 
GAATCGGATT TACTCGAGCT CTCCGCCAAA GATCTCGGCT ACATGTTCAG TTGCGACGGT 
GACCGCCTAT ACACCTACCT ACGCTACCTT CCGAGGATGG ATGTGCAAGC AAAGTTCAAG 
CCGATCACCT ACACGATCGT TCAGGTCGAG GTCACACTCA CGCCGACGTT CATCTGGAAC 
GACCAGATCC ACGGAAAGTC GGGACAACAA TCGTTCTATC TAGTGCTGGA GAATCTCAAC 
GAGAATCTTA TCATCCATCA GGAGAGAATC GGCATCGGGA AGATGAAGGT GAACCGGCAG 
GAGACTCAGA ATCTAGTTTT CACTATTCCA ATCGTCGACT GTCAGCTGAC GAACAATTTC 
CAACTTCGCC TCGCCAGCGA ATACTTTGTC ACCGACGACG TGGTGGTTCC GATGTCTCTT 
CACAATTGCA TTCTACCAAA GTCCTTCAAA AGCCACACAG ATCTTCTGGA CCTTGAGCCA 
CTTCCTATCA AAACTCTCAA AAACTCAAAG TTCGAGGCGA TCTACAACTT TGACTATTTC 
AACCCAATTC AAGCGCAAGT TTTCTTTTGC CTGTACAAAA CCGACAAAAG CGCATTGATC 
GGAGCGCCGA CAGGATCTGG AAAGACACTT TGCGCGGAGC TCGCCATGTT CCGCCTGCTT 
CAGGACCATC CTGGCATGAA AGTCGTGTAC ATTGCTCCGT TGAAGTCCTT GGTCCGAGAG 
CGAGTTGACG ATTGGAAGAA GAAGTTCGAA GATGGAATGG GATATCGAGT GGTCGAGGTG 
TCTGGGGATG TGACTCCAGA TCCCGAGGAG CTTGCGGCTT CCTCAATTCT TATTACGACA 
CCAGAAAAGT GGGATGGAAT CTCCAGAAGT TGGGCTACGA GGGAATACGT CCGACGAGTC 
GGCCTTATCG TGCTCGACGA GGTTCACCTA CTCGGCGTAG ATCGTGGAGC TGTGCTCGAG 
GCCATCGTCA GCAGACTGAA AATGATCACC AGAAGAAGCC ACGTGACAGA GGAGCCTGTC 
AGGTTGCTGG GTCTTTCCAC AGCGCTCGCC AACGCAGGAG ACGTCGCAGA GTGGCTTGGC 
ATCCCCGACG AGGCGTGCTA CAACTTCCGA CCTTCAGTTC GACCAGTGCC GATTTCAGTT 
CATATTCAAG GATTCCCAGG CCAACATTAC TGCCCACGAA TGGGACTGAT GAACAAGCCG 
GCTTACAAGG CAATTCTGAC CTATTCCCCA AGGAAGCCTG TGTTGATCTT CGTCTCGTCT 
AGACGACAAA CCCGCCTGAC AGCGCTCGCC TTTGTCAATT TGCTCATTGC CGACCACAAT 
CCGAAGCAAT GGCTCAACAT GGACATGCTC GAGTTGGAAG ATCTCATGGC CGCAATCAAG 
GATGAAAATC TCAAGTTGAC ACTACCATTC GGAATCGGAA TGCATCACGC TGGACTCAGC 
GCTCACGAGC GAGCCATTGT GGAGCAATTG TTTATCGAAA AGAAGATTCA AGTGCTCATC 
GCGACTGCCA CATTGGCGTG GGGAATCAAT TGCCCCGCTC ATTTAGTTAT TGTCAAGGGA 
ACCGAATATT TCGACGGAAA GAAGGGAAAA TACGTGGATT TTCCCGTTAC AGACGTTCTC 
CAAATGATGG GCCGCGCCGG ACGCCCACAA TTCGACGATT CGGCCGTTGC CGTGATCTAC 
GTACAGGACG CGAAAAAGAC GTTCTATAAG AAGTTCCTCT ACGAACCGTT CCCCGTCGAG 
TCCTCGCTCC TTCCAGTGCT GCCGAATCAC GTGAACGCCG AGATTTCCGC CGGAACAATC 
GATTCGAAGC AGGCGATCGT CGAGTATTTG TCGAAAACCT ATCTCTACAG GCGATTGTTT 
GCGAATCCCA ATTATTACGG TCTGGAGGAG GATTCCGAGG AGGCGATGCT CAAGTTTATC 
ACGAAAATCG TTGATGACTC TGTCGCAGAG CTCCTAGCTT CCGAGTGTAT CCACGTGGAT 
TCCGAACAGG ACGTGATCAA GCCGACGCCG TGTGGAAGGA TAGCTTCGGT GTACTATCTT 
CAGCACGAGA CCGTCCGTTT TCTAGTAAAA TCTCTACATT CTGGATGCTC GGTGGAGAAT 
ATGCTGAAGA TCCTGACCGA TGTTCCCGAG TACGCGGAGA TTCCTGTCCG CCACAACGAG 
GATCTCATCA ATACGGAACT TCAGAAGAAG CTTCGCATCC GATTCTCGAC GTCTGTGATG 
GGCACGTCTG CGTGCAAAGC CCACCTGCTC TTCCAGGCTC ATTTCATGCG GACTGTGCTC 
CCGACGGATT ACCGGACTGA TTTGAAGAGT GTGCTGGATC AGTGCATTCG GATTTTGCAG 
GCGATGCGTG AAATGGCTAG GCTCAAGAAT TGGCTCAGCG CAACGATGAA CATTATTCTG 
TTGCAGCAGA TGTGCCATTC GGCAAGGTGG CACGACGATC ATCCATTGCT CTGCCTACCT 
CATCTCTCCC ACGAGGATGC TCGTTCAATC GGCGATGGAA TGACAATTCC ACAGCTTCAA 
AACCATCTGG AAATCGAGAA ATCCACGTCA TTAGACGACG CGAAACTTGC TCGACGAGCC 
CAGAAACTCT TCAGAGAGTG CACCAAGTTA GATGAAGCTC AAAGCCGGGA AGTGCTGAAA 
GCTCTGTGCA ATTGGCCAAT TATCAACATG AAAATCATGC AATTAGTGGA TTCCCGCGGT 
AATTGTGTAG ATATTGATGA GACGAAGAAA CCGGTGAAAG TGACGGCTGG CGAGGTGTAC 
AAATTGAGAA TTGTCATGGA ACGAGTCGGA CCGGGAAAGA ACAACTCATC AATGCATCTT 
CCACAATGGC CAAAACCAAA ACAGGCTGGA TGGATAATTG TTGTGGGAAA CGTGTCGGCC 
GACATGATTC TTAACACGAC TACCGTAACC GGCTCACATT CGACTCGGTC GACCGCCAAA 
TTGGATATTC GTGCGCCGGC GACGAAAGGA AACCACGAGC TCGCCGTCCT AATTCTCAGC 
GATTGCTATT TGGGAATCGA TCAGGAGTAC ACTTTGCGAT TGGATGTCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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Y54E2A.4 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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