ORF » Species Summary » Caenorhabditis elegans » denn-4 cDNA ORF clone

denn-4 cDNA ORF clone, Caenorhabditis elegans

The following denn-4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the denn-4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa344509 NM_001307455.2
Latest version!		 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCa344510 NM_001307456.2
Latest version!		 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCa344511 NM_001307457.2
Latest version!		 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCa344512 NM_001307458.2
Latest version!		 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCa344513 NM_001307459.2
Latest version!		 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCa344514 NM_001307460.2
Latest version!		 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCa344515 NM_001307461.2
Latest version!		 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCa344516 NM_001307462.2
Latest version!		 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
View Old Accession Versions >>
OCa344514 NM_001307460.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCa344516 NM_001307462.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCa344510 NM_001307456.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCa344513 NM_001307459.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCa344509 NM_001307455.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCa344515 NM_001307461.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCa344511 NM_001307457.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCa344512 NM_001307458.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    denn-4 ( NM_001307455.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294384.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA.
    Price & Availability↑
    CloneID OCa344509
    Clone ID Related Accession (Same CDS sequence) NM_001307455.2 , NM_001307455.1
    Accession Version NM_001307455.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307455.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    GTCTTCATCC CGGCACCGGA GCCATTTTTG AATCAGGAGA CTGGCGAAGA GCTGCAATTT 
    GTCTATGGGG AATTCCCGTC GAAAATGCGA AGCGAGTACT TCCAACTGGA TCGACTTGAG 
    AAAAATGGGA AAGATGGGGA AAAGGTTCCC ATGCATTTTG TTTCAGAAGC ATCCCGTTGC 
    TCGGCAGTTC GCACAAAACC GGAAACCCGT TCCTCGTTGC TAGCCGCCAC AAATGCTGTG 
    AAAACCAACC CGCTTCATTG GTCAAAAACG CTTCTGTTCT ACTCATATTC ACTATGGTTC 
    ATGCAATTGG ATAGCCTCCT CACCGCTGCT CCAAACAAGA AGAAAATACT TCGTCTGGCA 
    TTTAATGTGC TCGATCGAAT GGAAAAAACC GAGATTTTCC CGCTCGATCA AGTATGCTAT 
    CGAATTCTTA TCGAACTTTG CGGAAAATAT TCGGAGCCCG AGATGGCTGT CAAGGTTATG 
    AGAGCCATGC AAAGAGCTGG GCTTGAACAG AACGCCGTCA CCTATGGAAT CTACCACCGC 
    GCCGTTATGG ATGCCACGTG GCCGTCGCCA GCTCGCCAAA AAGCCATAAA AATGTGGAAT 
    TTGCTGAGAA TTGTGCTCAC CGGCGCCGAA ACTCTCCGAA AATGGGCGAA TTTGGAAAGA 
    AGACCGCTGA AAATTGATGA AAAATCGGTG GAAAACACGG AAAATGCTGA GAATGTGCAG 
    GCGGAGATCA CGGTGGATTA TCAGATTACC AGCTATCCAC TGGATTCTGT GGAAAATTCG 
    CCGAAAATGA CCCCAAAACG GCACAATTTG GAGCAAAAAG AGCCGCTGAT GGATCCACTG 
    GGTGCTCTGG GCCCCCTGGA AGCTGAAAAT TTGGAAGTTT CACGCGAAAA AACCAAGGAA 
    CGATCAAAAT CGCTGGGAAA AGTTGGAATG TCGCCAGCGA GAGCGAAATT CTTTGCCGAT 
    CACGCTACAC TGCCATTTTC CAACGAAAAT ACGCCGAAAT CCGATAAAAA AGAGGCAAAA 
    AGTGGATGGT TTAAGGGAAT TGCGAATAGT CCAATGTTTA AAATGATTAA AAGTACTACT 
    TTTGATTCTT CCAAACAAAA CTCTGCTGAA TCATCAAGTG AAACTAGCTC AATAACCGGA 
    GCTTCTCCAT CATTCCATTC CGTAGTAAAT CAATTCAAAA AGGGATACGA TGACGTCATG 
    CCGTCTCGAT TCCGTCTCGG CGTTTCCACA CTATTGAACA AATCCGTCGG TGCAATGGGC 
    TCTGGAATGA TTTATTCGGA CGATCGGGCT TCTGCGGAAA ATCCGTTCTT TGTGAATCCA 
    AAAGATCCGG CTTATGTGCT AGATTCGGGC GTACCGAACT GTCTGCTGCG GGAGGAATAT 
    TGGATGCGTG AGGTGTATTT GCAGTTGAAA CGGAGCCGTG AGCGGGTTGA AAAGGGTCAG 
    AAAAGCGAGG AAAACGAGGA GATTTCCGAT GGAATAGCGG ATTTCCCCCA ATTCTCCGCA 
    AAAACTCCAG GAAAACTGGA GGTGATCCTG TGCACCGCCA CCTACTGCCC GTCATGCCGA 
    ACAATGGTCT ACGATGAGGA AATCATGGCC GGCTGGAAAG TTGACGATCA GAATTTGAAC 
    ACAATATGCC CACACTGCTC GGGAAGCGGA ACGGCGACAG ACGCCGTCTC CAACGAAACC 
    GCAGCCATTC AGGAGAGCGT TTTCGCGCCG AGACTCAAAA TTCGAATGAG ATGGATTGAG 
    GAGATGAAGA GTAGTTGGTA TCAGCCTGGT GGACTGCAGG AGACACCGAT TTCGACTCCC 
    GAAAAAACGA CGTCCACTCC CTCGGATATC CAGCTTGAAG TTGCATTTGT AAGCCCATTG 
    GTGCTCCGTC GTGAGCTTGA AACACTCCTA TCCACTGATA AGGACGCGAT GAGGAATGAA 
    TCGCTGAAAA CGACGCATCC GGTGGTTTTC TGGAATTTGA TATACTATTT GAGAAGACTC 
    TCCCTTCCGA GCCATTTATT CTCGTGGATT GCCGAACGTC ATCACATTCG TTGCGTCTTT 
    GATCTACCAA CACAACACGA CGAAACCCGT CTCCCATTGT ACTTTTACAA CCCGAATCAC 
    GATGGAAACG CGCCAAGTTT CGGCGATTTT ACCGAAAAAA TCACCCGAAA ATTGGAGGAT 
    GGCTCGGATT CGACGGGCTA TGCTACATGG AAGACGGTCA CCGACTCGGT GAACAAGAAT 
    TTGCTGTTTA AGGCAGTTCA GACACTGGTC AACTCGTCGC GATTGGTGAA AAATGGAAGT 
    GTTCATGTTG GAGCACATTT TCCCATTTTT AGAGACATTC AATTCGCATC GATTGACGTA 
    TTTGGCAGAG CACTGCTTCG TGATGCTCTC GACACTCAAT ACGACTCGGA ATCCAACAAA 
    TTACCGCCGA AAATCAAGGA AATCCTTCCG CTTCAGGATC ATCCGTTGTC CCATGTCACC 
    CGAGCGTGCC GCAAAGTTTT CATGCCATTG GACCTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294384.1
    CDS8..4609
    Translation

    Target ORF information:

    RefSeq Version NM_001307455.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307455.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    GTCTTCATCC CGGCACCGGA GCCATTTTTG AATCAGGAGA CTGGCGAAGA GCTGCAATTT 
    GTCTATGGGG AATTCCCGTC GAAAATGCGA AGCGAGTACT TCCAACTGGA TCGACTTGAG 
    AAAAATGGGA AAGATGGGGA AAAGGTTCCC ATGCATTTTG TTTCAGAAGC ATCCCGTTGC 
    TCGGCAGTTC GCACAAAACC GGAAACCCGT TCCTCGTTGC TAGCCGCCAC AAATGCTGTG 
    AAAACCAACC CGCTTCATTG GTCAAAAACG CTTCTGTTCT ACTCATATTC ACTATGGTTC 
    ATGCAATTGG ATAGCCTCCT CACCGCTGCT CCAAACAAGA AGAAAATACT TCGTCTGGCA 
    TTTAATGTGC TCGATCGAAT GGAAAAAACC GAGATTTTCC CGCTCGATCA AGTATGCTAT 
    CGAATTCTTA TCGAACTTTG CGGAAAATAT TCGGAGCCCG AGATGGCTGT CAAGGTTATG 
    AGAGCCATGC AAAGAGCTGG GCTTGAACAG AACGCCGTCA CCTATGGAAT CTACCACCGC 
    GCCGTTATGG ATGCCACGTG GCCGTCGCCA GCTCGCCAAA AAGCCATAAA AATGTGGAAT 
    TTGCTGAGAA TTGTGCTCAC CGGCGCCGAA ACTCTCCGAA AATGGGCGAA TTTGGAAAGA 
    AGACCGCTGA AAATTGATGA AAAATCGGTG GAAAACACGG AAAATGCTGA GAATGTGCAG 
    GCGGAGATCA CGGTGGATTA TCAGATTACC AGCTATCCAC TGGATTCTGT GGAAAATTCG 
    CCGAAAATGA CCCCAAAACG GCACAATTTG GAGCAAAAAG AGCCGCTGAT GGATCCACTG 
    GGTGCTCTGG GCCCCCTGGA AGCTGAAAAT TTGGAAGTTT CACGCGAAAA AACCAAGGAA 
    CGATCAAAAT CGCTGGGAAA AGTTGGAATG TCGCCAGCGA GAGCGAAATT CTTTGCCGAT 
    CACGCTACAC TGCCATTTTC CAACGAAAAT ACGCCGAAAT CCGATAAAAA AGAGGCAAAA 
    AGTGGATGGT TTAAGGGAAT TGCGAATAGT CCAATGTTTA AAATGATTAA AAGTACTACT 
    TTTGATTCTT CCAAACAAAA CTCTGCTGAA TCATCAAGTG AAACTAGCTC AATAACCGGA 
    GCTTCTCCAT CATTCCATTC CGTAGTAAAT CAATTCAAAA AGGGATACGA TGACGTCATG 
    CCGTCTCGAT TCCGTCTCGG CGTTTCCACA CTATTGAACA AATCCGTCGG TGCAATGGGC 
    TCTGGAATGA TTTATTCGGA CGATCGGGCT TCTGCGGAAA ATCCGTTCTT TGTGAATCCA 
    AAAGATCCGG CTTATGTGCT AGATTCGGGC GTACCGAACT GTCTGCTGCG GGAGGAATAT 
    TGGATGCGTG AGGTGTATTT GCAGTTGAAA CGGAGCCGTG AGCGGGTTGA AAAGGGTCAG 
    AAAAGCGAGG AAAACGAGGA GATTTCCGAT GGAATAGCGG ATTTCCCCCA ATTCTCCGCA 
    AAAACTCCAG GAAAACTGGA GGTGATCCTG TGCACCGCCA CCTACTGCCC GTCATGCCGA 
    ACAATGGTCT ACGATGAGGA AATCATGGCC GGCTGGAAAG TTGACGATCA GAATTTGAAC 
    ACAATATGCC CACACTGCTC GGGAAGCGGA ACGGCGACAG ACGCCGTCTC CAACGAAACC 
    GCAGCCATTC AGGAGAGCGT TTTCGCGCCG AGACTCAAAA TTCGAATGAG ATGGATTGAG 
    GAGATGAAGA GTAGTTGGTA TCAGCCTGGT GGACTGCAGG AGACACCGAT TTCGACTCCC 
    GAAAAAACGA CGTCCACTCC CTCGGATATC CAGCTTGAAG TTGCATTTGT AAGCCCATTG 
    GTGCTCCGTC GTGAGCTTGA AACACTCCTA TCCACTGATA AGGACGCGAT GAGGAATGAA 
    TCGCTGAAAA CGACGCATCC GGTGGTTTTC TGGAATTTGA TATACTATTT GAGAAGACTC 
    TCCCTTCCGA GCCATTTATT CTCGTGGATT GCCGAACGTC ATCACATTCG TTGCGTCTTT 
    GATCTACCAA CACAACACGA CGAAACCCGT CTCCCATTGT ACTTTTACAA CCCGAATCAC 
    GATGGAAACG CGCCAAGTTT CGGCGATTTT ACCGAAAAAA TCACCCGAAA ATTGGAGGAT 
    GGCTCGGATT CGACGGGCTA TGCTACATGG AAGACGGTCA CCGACTCGGT GAACAAGAAT 
    TTGCTGTTTA AGGCAGTTCA GACACTGGTC AACTCGTCGC GATTGGTGAA AAATGGAAGT 
    GTTCATGTTG GAGCACATTT TCCCATTTTT AGAGACATTC AATTCGCATC GATTGACGTA 
    TTTGGCAGAG CACTGCTTCG TGATGCTCTC GACACTCAAT ACGACTCGGA ATCCAACAAA 
    TTACCGCCGA AAATCAAGGA AATCCTTCCG CTTCAGGATC ATCCGTTGTC CCATGTCACC 
    CGAGCGTGCC GCAAAGTTTT CATGCCATTG GACCTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307455.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294384.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.
    Price & Availability↑
    CloneID OCa344509
    Clone ID Related Accession (Same CDS sequence) NM_001307455.2 , NM_001307455.1
    Accession Version NM_001307455.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    GTCTTCATCC CGGCACCGGA GCCATTTTTG AATCAGGAGA CTGGCGAAGA GCTGCAATTT 
    GTCTATGGGG AATTCCCGTC GAAAATGCGA AGCGAGTACT TCCAACTGGA TCGACTTGAG 
    AAAAATGGGA AAGATGGGGA AAAGGTTCCC ATGCATTTTG TTTCAGAAGC ATCCCGTTGC 
    TCGGCAGTTC GCACAAAACC GGAAACCCGT TCCTCGTTGC TAGCCGCCAC AAATGCTGTG 
    AAAACCAACC CGCTTCATTG GTCAAAAACG CTTCTGTTCT ACTCATATTC ACTATGGTTC 
    ATGCAATTGG ATAGCCTCCT CACCGCTGCT CCAAACAAGA AGAAAATACT TCGTCTGGCA 
    TTTAATGTGC TCGATCGAAT GGAAAAAACC GAGATTTTCC CGCTCGATCA AGTATGCTAT 
    CGAATTCTTA TCGAACTTTG CGGAAAATAT TCGGAGCCCG AGATGGCTGT CAAGGTTATG 
    AGAGCCATGC AAAGAGCTGG GCTTGAACAG AACGCCGTCA CCTATGGAAT CTACCACCGC 
    GCCGTTATGG ATGCCACGTG GCCGTCGCCA GCTCGCCAAA AAGCCATAAA AATGTGGAAT 
    TTGCTGAGAA TTGTGCTCAC CGGCGCCGAA ACTCTCCGAA AATGGGCGAA TTTGGAAAGA 
    AGACCGCTGA AAATTGATGA AAAATCGGTG GAAAACACGG AAAATGCTGA GAATGTGCAG 
    GCGGAGATCA CGGTGGATTA TCAGATTACC AGCTATCCAC TGGATTCTGT GGAAAATTCG 
    CCGAAAATGA CCCCAAAACG GCACAATTTG GAGCAAAAAG AGCCGCTGAT GGATCCACTG 
    GGTGCTCTGG GCCCCCTGGA AGCTGAAAAT TTGGAAGTTT CACGCGAAAA AACCAAGGAA 
    CGATCAAAAT CGCTGGGAAA AGTTGGAATG TCGCCAGCGA GAGCGAAATT CTTTGCCGAT 
    CACGCTACAC TGCCATTTTC CAACGAAAAT ACGCCGAAAT CCGATAAAAA AGAGGCAAAA 
    AGTGGATGGT TTAAGGGAAT TGCGAATAGT CCAATGTTTA AAATGATTAA AAGTACTACT 
    TTTGATTCTT CCAAACAAAA CTCTGCTGAA TCATCAAGTG AAACTAGCTC AATAACCGGA 
    GCTTCTCCAT CATTCCATTC CGTAGTAAAT CAATTCAAAA AGGGATACGA TGACGTCATG 
    CCGTCTCGAT TCCGTCTCGG CGTTTCCACA CTATTGAACA AATCCGTCGG TGCAATGGGC 
    TCTGGAATGA TTTATTCGGA CGATCGGGCT TCTGCGGAAA ATCCGTTCTT TGTGAATCCA 
    AAAGATCCGG CTTATGTGCT AGATTCGGGC GTACCGAACT GTCTGCTGCG GGAGGAATAT 
    TGGATGCGTG AGGTGTATTT GCAGTTGAAA CGGAGCCGTG AGCGGGTTGA AAAGGGTCAG 
    AAAAGCGAGG AAAACGAGGA GATTTCCGAT GGAATAGCGG ATTTCCCCCA ATTCTCCGCA 
    AAAACTCCAG GAAAACTGGA GGTGATCCTG TGCACCGCCA CCTACTGCCC GTCATGCCGA 
    ACAATGGTCT ACGATGAGGA AATCATGGCC GGCTGGAAAG TTGACGATCA GAATTTGAAC 
    ACAATATGCC CACACTGCTC GGGAAGCGGA ACGGCGACAG ACGCCGTCTC CAACGAAACC 
    GCAGCCATTC AGGAGAGCGT TTTCGCGCCG AGACTCAAAA TTCGAATGAG ATGGATTGAG 
    GAGATGAAGA GTAGTTGGTA TCAGCCTGGT GGACTGCAGG AGACACCGAT TTCGACTCCC 
    GAAAAAACGA CGTCCACTCC CTCGGATATC CAGCTTGAAG TTGCATTTGT AAGCCCATTG 
    GTGCTCCGTC GTGAGCTTGA AACACTCCTA TCCACTGATA AGGACGCGAT GAGGAATGAA 
    TCGCTGAAAA CGACGCATCC GGTGGTTTTC TGGAATTTGA TATACTATTT GAGAAGACTC 
    TCCCTTCCGA GCCATTTATT CTCGTGGATT GCCGAACGTC ATCACATTCG TTGCGTCTTT 
    GATCTACCAA CACAACACGA CGAAACCCGT CTCCCATTGT ACTTTTACAA CCCGAATCAC 
    GATGGAAACG CGCCAAGTTT CGGCGATTTT ACCGAAAAAA TCACCCGAAA ATTGGAGGAT 
    GGCTCGGATT CGACGGGCTA TGCTACATGG AAGACGGTCA CCGACTCGGT GAACAAGAAT 
    TTGCTGTTTA AGGCAGTTCA GACACTGGTC AACTCGTCGC GATTGGTGAA AAATGGAAGT 
    GTTCATGTTG GAGCACATTT TCCCATTTTT AGAGACATTC AATTCGCATC GATTGACGTA 
    TTTGGCAGAG CACTGCTTCG TGATGCTCTC GACACTCAAT ACGACTCGGA ATCCAACAAA 
    TTACCGCCGA AAATCAAGGA AATCCTTCCG CTTCAGGATC ATCCGTTGTC CCATGTCACC 
    CGAGCGTGCC GCAAAGTTTT CATGCCATTG GACCTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294384.1
    CDS1..4602
    Translation

    Target ORF information:

    RefSeq Version NM_001307455.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307455.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    GTCTTCATCC CGGCACCGGA GCCATTTTTG AATCAGGAGA CTGGCGAAGA GCTGCAATTT 
    GTCTATGGGG AATTCCCGTC GAAAATGCGA AGCGAGTACT TCCAACTGGA TCGACTTGAG 
    AAAAATGGGA AAGATGGGGA AAAGGTTCCC ATGCATTTTG TTTCAGAAGC ATCCCGTTGC 
    TCGGCAGTTC GCACAAAACC GGAAACCCGT TCCTCGTTGC TAGCCGCCAC AAATGCTGTG 
    AAAACCAACC CGCTTCATTG GTCAAAAACG CTTCTGTTCT ACTCATATTC ACTATGGTTC 
    ATGCAATTGG ATAGCCTCCT CACCGCTGCT CCAAACAAGA AGAAAATACT TCGTCTGGCA 
    TTTAATGTGC TCGATCGAAT GGAAAAAACC GAGATTTTCC CGCTCGATCA AGTATGCTAT 
    CGAATTCTTA TCGAACTTTG CGGAAAATAT TCGGAGCCCG AGATGGCTGT CAAGGTTATG 
    AGAGCCATGC AAAGAGCTGG GCTTGAACAG AACGCCGTCA CCTATGGAAT CTACCACCGC 
    GCCGTTATGG ATGCCACGTG GCCGTCGCCA GCTCGCCAAA AAGCCATAAA AATGTGGAAT 
    TTGCTGAGAA TTGTGCTCAC CGGCGCCGAA ACTCTCCGAA AATGGGCGAA TTTGGAAAGA 
    AGACCGCTGA AAATTGATGA AAAATCGGTG GAAAACACGG AAAATGCTGA GAATGTGCAG 
    GCGGAGATCA CGGTGGATTA TCAGATTACC AGCTATCCAC TGGATTCTGT GGAAAATTCG 
    CCGAAAATGA CCCCAAAACG GCACAATTTG GAGCAAAAAG AGCCGCTGAT GGATCCACTG 
    GGTGCTCTGG GCCCCCTGGA AGCTGAAAAT TTGGAAGTTT CACGCGAAAA AACCAAGGAA 
    CGATCAAAAT CGCTGGGAAA AGTTGGAATG TCGCCAGCGA GAGCGAAATT CTTTGCCGAT 
    CACGCTACAC TGCCATTTTC CAACGAAAAT ACGCCGAAAT CCGATAAAAA AGAGGCAAAA 
    AGTGGATGGT TTAAGGGAAT TGCGAATAGT CCAATGTTTA AAATGATTAA AAGTACTACT 
    TTTGATTCTT CCAAACAAAA CTCTGCTGAA TCATCAAGTG AAACTAGCTC AATAACCGGA 
    GCTTCTCCAT CATTCCATTC CGTAGTAAAT CAATTCAAAA AGGGATACGA TGACGTCATG 
    CCGTCTCGAT TCCGTCTCGG CGTTTCCACA CTATTGAACA AATCCGTCGG TGCAATGGGC 
    TCTGGAATGA TTTATTCGGA CGATCGGGCT TCTGCGGAAA ATCCGTTCTT TGTGAATCCA 
    AAAGATCCGG CTTATGTGCT AGATTCGGGC GTACCGAACT GTCTGCTGCG GGAGGAATAT 
    TGGATGCGTG AGGTGTATTT GCAGTTGAAA CGGAGCCGTG AGCGGGTTGA AAAGGGTCAG 
    AAAAGCGAGG AAAACGAGGA GATTTCCGAT GGAATAGCGG ATTTCCCCCA ATTCTCCGCA 
    AAAACTCCAG GAAAACTGGA GGTGATCCTG TGCACCGCCA CCTACTGCCC GTCATGCCGA 
    ACAATGGTCT ACGATGAGGA AATCATGGCC GGCTGGAAAG TTGACGATCA GAATTTGAAC 
    ACAATATGCC CACACTGCTC GGGAAGCGGA ACGGCGACAG ACGCCGTCTC CAACGAAACC 
    GCAGCCATTC AGGAGAGCGT TTTCGCGCCG AGACTCAAAA TTCGAATGAG ATGGATTGAG 
    GAGATGAAGA GTAGTTGGTA TCAGCCTGGT GGACTGCAGG AGACACCGAT TTCGACTCCC 
    GAAAAAACGA CGTCCACTCC CTCGGATATC CAGCTTGAAG TTGCATTTGT AAGCCCATTG 
    GTGCTCCGTC GTGAGCTTGA AACACTCCTA TCCACTGATA AGGACGCGAT GAGGAATGAA 
    TCGCTGAAAA CGACGCATCC GGTGGTTTTC TGGAATTTGA TATACTATTT GAGAAGACTC 
    TCCCTTCCGA GCCATTTATT CTCGTGGATT GCCGAACGTC ATCACATTCG TTGCGTCTTT 
    GATCTACCAA CACAACACGA CGAAACCCGT CTCCCATTGT ACTTTTACAA CCCGAATCAC 
    GATGGAAACG CGCCAAGTTT CGGCGATTTT ACCGAAAAAA TCACCCGAAA ATTGGAGGAT 
    GGCTCGGATT CGACGGGCTA TGCTACATGG AAGACGGTCA CCGACTCGGT GAACAAGAAT 
    TTGCTGTTTA AGGCAGTTCA GACACTGGTC AACTCGTCGC GATTGGTGAA AAATGGAAGT 
    GTTCATGTTG GAGCACATTT TCCCATTTTT AGAGACATTC AATTCGCATC GATTGACGTA 
    TTTGGCAGAG CACTGCTTCG TGATGCTCTC GACACTCAAT ACGACTCGGA ATCCAACAAA 
    TTACCGCCGA AAATCAAGGA AATCCTTCCG CTTCAGGATC ATCCGTTGTC CCATGTCACC 
    CGAGCGTGCC GCAAAGTTTT CATGCCATTG GACCTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307456.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294385.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.
    Price & Availability↑
    CloneID OCa344510
    Clone ID Related Accession (Same CDS sequence) NM_001307456.1 , NM_001307456.2
    Accession Version NM_001307456.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4608bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGGTCT TCATCCCGGC ACCGGAGCCA TTTTTGAATC AGGAGACTGG CGAAGAGCTG 
    CAATTTGTCT ATGGGGAATT CCCGTCGAAA ATGCGAAGCG AGTACTTCCA ACTGGATCGA 
    CTTGAGAAAA ATGGGAAAGA TGGGGAAAAG GTTCCCATGC ATTTTGTTTC AGAAGCATCC 
    CGTTGCTCGG CAGTTCGCAC AAAACCGGAA ACCCGTTCCT CGTTGCTAGC CGCCACAAAT 
    GCTGTGAAAA CCAACCCGCT TCATTGGTCA AAAACGCTTC TGTTCTACTC ATATTCACTA 
    TGGTTCATGC AATTGGATAG CCTCCTCACC GCTGCTCCAA ACAAGAAGAA AATACTTCGT 
    CTGGCATTTA ATGTGCTCGA TCGAATGGAA AAAACCGAGA TTTTCCCGCT CGATCAAGTA 
    TGCTATCGAA TTCTTATCGA ACTTTGCGGA AAATATTCGG AGCCCGAGAT GGCTGTCAAG 
    GTTATGAGAG CCATGCAAAG AGCTGGGCTT GAACAGAACG CCGTCACCTA TGGAATCTAC 
    CACCGCGCCG TTATGGATGC CACGTGGCCG TCGCCAGCTC GCCAAAAAGC CATAAAAATG 
    TGGAATTTGC TGAGAATTGT GCTCACCGGC GCCGAAACTC TCCGAAAATG GGCGAATTTG 
    GAAAGAAGAC CGCTGAAAAT TGATGAAAAA TCGGTGGAAA ACACGGAAAA TGCTGAGAAT 
    GTGCAGGCGG AGATCACGGT GGATTATCAG ATTACCAGCT ATCCACTGGA TTCTGTGGAA 
    AATTCGCCGA AAATGACCCC AAAACGGCAC AATTTGGAGC AAAAAGAGCC GCTGATGGAT 
    CCACTGGGTG CTCTGGGCCC CCTGGAAGCT GAAAATTTGG AAGTTTCACG CGAAAAAACC 
    AAGGAACGAT CAAAATCGCT GGGAAAAGTT GGAATGTCGC CAGCGAGAGC GAAATTCTTT 
    GCCGATCACG CTACACTGCC ATTTTCCAAC GAAAATACGC CGAAATCCGA TAAAAAAGAG 
    GCAAAAAGTG GATGGTTTAA GGGAATTGCG AATAGTCCAA TGTTTAAAAT GATTAAAAGT 
    ACTACTTTTG ATTCTTCCAA ACAAAACTCT GCTGAATCAT CAAGTGAAAC TAGCTCAATA 
    ACCGGAGCTT CTCCATCATT CCATTCCGTA GTAAATCAAT TCAAAAAGGG ATACGATGAC 
    GTCATGCCGT CTCGATTCCG TCTCGGCGTT TCCACACTAT TGAACAAATC CGTCGGTGCA 
    ATGGGCTCTG GAATGATTTA TTCGGACGAT CGGGCTTCTG CGGAAAATCC GTTCTTTGTG 
    AATCCAAAAG ATCCGGCTTA TGTGCTAGAT TCGGGCGTAC CGAACTGTCT GCTGCGGGAG 
    GAATATTGGA TGCGTGAGGT GTATTTGCAG TTGAAACGGA GCCGTGAGCG GGTTGAAAAG 
    GGTCAGAAAA GCGAGGAAAA CGAGGAGATT TCCGATGGAA TAGCGGATTT CCCCCAATTC 
    TCCGCAAAAA CTCCAGGAAA ACTGGAGGTG ATCCTGTGCA CCGCCACCTA CTGCCCGTCA 
    TGCCGAACAA TGGTCTACGA TGAGGAAATC ATGGCCGGCT GGAAAGTTGA CGATCAGAAT 
    TTGAACACAA TATGCCCACA CTGCTCGGGA AGCGGAACGG CGACAGACGC CGTCTCCAAC 
    GAAACCGCAG CCATTCAGGA GAGCGTTTTC GCGCCGAGAC TCAAAATTCG AATGAGATGG 
    ATTGAGGAGA TGAAGAGTAG TTGGTATCAG CCTGGTGGAC TGCAGGAGAC ACCGATTTCG 
    ACTCCCGAAA AAACGACGTC CACTCCCTCG GATATCCAGC TTGAAGTTGC ATTTGTAAGC 
    CCATTGGTGC TCCGTCGTGA GCTTGAAACA CTCCTATCCA CTGATAAGGA CGCGATGAGG 
    AATGAATCGC TGAAAACGAC GCATCCGGTG GTTTTCTGGA ATTTGATATA CTATTTGAGA 
    AGACTCTCCC TTCCGAGCCA TTTATTCTCG TGGATTGCCG AACGTCATCA CATTCGTTGC 
    GTCTTTGATC TACCAACACA ACACGACGAA ACCCGTCTCC CATTGTACTT TTACAACCCG 
    AATCACGATG GAAACGCGCC AAGTTTCGGC GATTTTACCG AAAAAATCAC CCGAAAATTG 
    GAGGATGGCT CGGATTCGAC GGGCTATGCT ACATGGAAGA CGGTCACCGA CTCGGTGAAC 
    AAGAATTTGC TGTTTAAGGC AGTTCAGACA CTGGTCAACT CGTCGCGATT GGTGAAAAAT 
    GGAAGTGTTC ATGTTGGAGC ACATTTTCCC ATTTTTAGAG ACATTCAATT CGCATCGATT 
    GACGTATTTG GCAGAGCACT GCTTCGTGAT GCTCTCGACA CTCAATACGA CTCGGAATCC 
    AACAAATTAC CGCCGAAAAT CAAGGAAATC CTTCCGCTTC AGGATCATCC GTTGTCCCAT 
    GTCACCCGAG CGTGCCGCAA AGTTTTCATG CCATTGGACC TATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294385.1
    CDS1..4608
    Translation

    Target ORF information:

    RefSeq Version NM_001307456.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307456.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGGTCT TCATCCCGGC ACCGGAGCCA TTTTTGAATC AGGAGACTGG CGAAGAGCTG 
    CAATTTGTCT ATGGGGAATT CCCGTCGAAA ATGCGAAGCG AGTACTTCCA ACTGGATCGA 
    CTTGAGAAAA ATGGGAAAGA TGGGGAAAAG GTTCCCATGC ATTTTGTTTC AGAAGCATCC 
    CGTTGCTCGG CAGTTCGCAC AAAACCGGAA ACCCGTTCCT CGTTGCTAGC CGCCACAAAT 
    GCTGTGAAAA CCAACCCGCT TCATTGGTCA AAAACGCTTC TGTTCTACTC ATATTCACTA 
    TGGTTCATGC AATTGGATAG CCTCCTCACC GCTGCTCCAA ACAAGAAGAA AATACTTCGT 
    CTGGCATTTA ATGTGCTCGA TCGAATGGAA AAAACCGAGA TTTTCCCGCT CGATCAAGTA 
    TGCTATCGAA TTCTTATCGA ACTTTGCGGA AAATATTCGG AGCCCGAGAT GGCTGTCAAG 
    GTTATGAGAG CCATGCAAAG AGCTGGGCTT GAACAGAACG CCGTCACCTA TGGAATCTAC 
    CACCGCGCCG TTATGGATGC CACGTGGCCG TCGCCAGCTC GCCAAAAAGC CATAAAAATG 
    TGGAATTTGC TGAGAATTGT GCTCACCGGC GCCGAAACTC TCCGAAAATG GGCGAATTTG 
    GAAAGAAGAC CGCTGAAAAT TGATGAAAAA TCGGTGGAAA ACACGGAAAA TGCTGAGAAT 
    GTGCAGGCGG AGATCACGGT GGATTATCAG ATTACCAGCT ATCCACTGGA TTCTGTGGAA 
    AATTCGCCGA AAATGACCCC AAAACGGCAC AATTTGGAGC AAAAAGAGCC GCTGATGGAT 
    CCACTGGGTG CTCTGGGCCC CCTGGAAGCT GAAAATTTGG AAGTTTCACG CGAAAAAACC 
    AAGGAACGAT CAAAATCGCT GGGAAAAGTT GGAATGTCGC CAGCGAGAGC GAAATTCTTT 
    GCCGATCACG CTACACTGCC ATTTTCCAAC GAAAATACGC CGAAATCCGA TAAAAAAGAG 
    GCAAAAAGTG GATGGTTTAA GGGAATTGCG AATAGTCCAA TGTTTAAAAT GATTAAAAGT 
    ACTACTTTTG ATTCTTCCAA ACAAAACTCT GCTGAATCAT CAAGTGAAAC TAGCTCAATA 
    ACCGGAGCTT CTCCATCATT CCATTCCGTA GTAAATCAAT TCAAAAAGGG ATACGATGAC 
    GTCATGCCGT CTCGATTCCG TCTCGGCGTT TCCACACTAT TGAACAAATC CGTCGGTGCA 
    ATGGGCTCTG GAATGATTTA TTCGGACGAT CGGGCTTCTG CGGAAAATCC GTTCTTTGTG 
    AATCCAAAAG ATCCGGCTTA TGTGCTAGAT TCGGGCGTAC CGAACTGTCT GCTGCGGGAG 
    GAATATTGGA TGCGTGAGGT GTATTTGCAG TTGAAACGGA GCCGTGAGCG GGTTGAAAAG 
    GGTCAGAAAA GCGAGGAAAA CGAGGAGATT TCCGATGGAA TAGCGGATTT CCCCCAATTC 
    TCCGCAAAAA CTCCAGGAAA ACTGGAGGTG ATCCTGTGCA CCGCCACCTA CTGCCCGTCA 
    TGCCGAACAA TGGTCTACGA TGAGGAAATC ATGGCCGGCT GGAAAGTTGA CGATCAGAAT 
    TTGAACACAA TATGCCCACA CTGCTCGGGA AGCGGAACGG CGACAGACGC CGTCTCCAAC 
    GAAACCGCAG CCATTCAGGA GAGCGTTTTC GCGCCGAGAC TCAAAATTCG AATGAGATGG 
    ATTGAGGAGA TGAAGAGTAG TTGGTATCAG CCTGGTGGAC TGCAGGAGAC ACCGATTTCG 
    ACTCCCGAAA AAACGACGTC CACTCCCTCG GATATCCAGC TTGAAGTTGC ATTTGTAAGC 
    CCATTGGTGC TCCGTCGTGA GCTTGAAACA CTCCTATCCA CTGATAAGGA CGCGATGAGG 
    AATGAATCGC TGAAAACGAC GCATCCGGTG GTTTTCTGGA ATTTGATATA CTATTTGAGA 
    AGACTCTCCC TTCCGAGCCA TTTATTCTCG TGGATTGCCG AACGTCATCA CATTCGTTGC 
    GTCTTTGATC TACCAACACA ACACGACGAA ACCCGTCTCC CATTGTACTT TTACAACCCG 
    AATCACGATG GAAACGCGCC AAGTTTCGGC GATTTTACCG AAAAAATCAC CCGAAAATTG 
    GAGGATGGCT CGGATTCGAC GGGCTATGCT ACATGGAAGA CGGTCACCGA CTCGGTGAAC 
    AAGAATTTGC TGTTTAAGGC AGTTCAGACA CTGGTCAACT CGTCGCGATT GGTGAAAAAT 
    GGAAGTGTTC ATGTTGGAGC ACATTTTCCC ATTTTTAGAG ACATTCAATT CGCATCGATT 
    GACGTATTTG GCAGAGCACT GCTTCGTGAT GCTCTCGACA CTCAATACGA CTCGGAATCC 
    AACAAATTAC CGCCGAAAAT CAAGGAAATC CTTCCGCTTC AGGATCATCC GTTGTCCCAT 
    GTCACCCGAG CGTGCCGCAA AGTTTTCATG CCATTGGACC TATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307456.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294385.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA.
    Price & Availability↑
    CloneID OCa344510
    Clone ID Related Accession (Same CDS sequence) NM_001307456.1 , NM_001307456.2
    Accession Version NM_001307456.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4608bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307456.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGGTCT TCATCCCGGC ACCGGAGCCA TTTTTGAATC AGGAGACTGG CGAAGAGCTG 
    CAATTTGTCT ATGGGGAATT CCCGTCGAAA ATGCGAAGCG AGTACTTCCA ACTGGATCGA 
    CTTGAGAAAA ATGGGAAAGA TGGGGAAAAG GTTCCCATGC ATTTTGTTTC AGAAGCATCC 
    CGTTGCTCGG CAGTTCGCAC AAAACCGGAA ACCCGTTCCT CGTTGCTAGC CGCCACAAAT 
    GCTGTGAAAA CCAACCCGCT TCATTGGTCA AAAACGCTTC TGTTCTACTC ATATTCACTA 
    TGGTTCATGC AATTGGATAG CCTCCTCACC GCTGCTCCAA ACAAGAAGAA AATACTTCGT 
    CTGGCATTTA ATGTGCTCGA TCGAATGGAA AAAACCGAGA TTTTCCCGCT CGATCAAGTA 
    TGCTATCGAA TTCTTATCGA ACTTTGCGGA AAATATTCGG AGCCCGAGAT GGCTGTCAAG 
    GTTATGAGAG CCATGCAAAG AGCTGGGCTT GAACAGAACG CCGTCACCTA TGGAATCTAC 
    CACCGCGCCG TTATGGATGC CACGTGGCCG TCGCCAGCTC GCCAAAAAGC CATAAAAATG 
    TGGAATTTGC TGAGAATTGT GCTCACCGGC GCCGAAACTC TCCGAAAATG GGCGAATTTG 
    GAAAGAAGAC CGCTGAAAAT TGATGAAAAA TCGGTGGAAA ACACGGAAAA TGCTGAGAAT 
    GTGCAGGCGG AGATCACGGT GGATTATCAG ATTACCAGCT ATCCACTGGA TTCTGTGGAA 
    AATTCGCCGA AAATGACCCC AAAACGGCAC AATTTGGAGC AAAAAGAGCC GCTGATGGAT 
    CCACTGGGTG CTCTGGGCCC CCTGGAAGCT GAAAATTTGG AAGTTTCACG CGAAAAAACC 
    AAGGAACGAT CAAAATCGCT GGGAAAAGTT GGAATGTCGC CAGCGAGAGC GAAATTCTTT 
    GCCGATCACG CTACACTGCC ATTTTCCAAC GAAAATACGC CGAAATCCGA TAAAAAAGAG 
    GCAAAAAGTG GATGGTTTAA GGGAATTGCG AATAGTCCAA TGTTTAAAAT GATTAAAAGT 
    ACTACTTTTG ATTCTTCCAA ACAAAACTCT GCTGAATCAT CAAGTGAAAC TAGCTCAATA 
    ACCGGAGCTT CTCCATCATT CCATTCCGTA GTAAATCAAT TCAAAAAGGG ATACGATGAC 
    GTCATGCCGT CTCGATTCCG TCTCGGCGTT TCCACACTAT TGAACAAATC CGTCGGTGCA 
    ATGGGCTCTG GAATGATTTA TTCGGACGAT CGGGCTTCTG CGGAAAATCC GTTCTTTGTG 
    AATCCAAAAG ATCCGGCTTA TGTGCTAGAT TCGGGCGTAC CGAACTGTCT GCTGCGGGAG 
    GAATATTGGA TGCGTGAGGT GTATTTGCAG TTGAAACGGA GCCGTGAGCG GGTTGAAAAG 
    GGTCAGAAAA GCGAGGAAAA CGAGGAGATT TCCGATGGAA TAGCGGATTT CCCCCAATTC 
    TCCGCAAAAA CTCCAGGAAA ACTGGAGGTG ATCCTGTGCA CCGCCACCTA CTGCCCGTCA 
    TGCCGAACAA TGGTCTACGA TGAGGAAATC ATGGCCGGCT GGAAAGTTGA CGATCAGAAT 
    TTGAACACAA TATGCCCACA CTGCTCGGGA AGCGGAACGG CGACAGACGC CGTCTCCAAC 
    GAAACCGCAG CCATTCAGGA GAGCGTTTTC GCGCCGAGAC TCAAAATTCG AATGAGATGG 
    ATTGAGGAGA TGAAGAGTAG TTGGTATCAG CCTGGTGGAC TGCAGGAGAC ACCGATTTCG 
    ACTCCCGAAA AAACGACGTC CACTCCCTCG GATATCCAGC TTGAAGTTGC ATTTGTAAGC 
    CCATTGGTGC TCCGTCGTGA GCTTGAAACA CTCCTATCCA CTGATAAGGA CGCGATGAGG 
    AATGAATCGC TGAAAACGAC GCATCCGGTG GTTTTCTGGA ATTTGATATA CTATTTGAGA 
    AGACTCTCCC TTCCGAGCCA TTTATTCTCG TGGATTGCCG AACGTCATCA CATTCGTTGC 
    GTCTTTGATC TACCAACACA ACACGACGAA ACCCGTCTCC CATTGTACTT TTACAACCCG 
    AATCACGATG GAAACGCGCC AAGTTTCGGC GATTTTACCG AAAAAATCAC CCGAAAATTG 
    GAGGATGGCT CGGATTCGAC GGGCTATGCT ACATGGAAGA CGGTCACCGA CTCGGTGAAC 
    AAGAATTTGC TGTTTAAGGC AGTTCAGACA CTGGTCAACT CGTCGCGATT GGTGAAAAAT 
    GGAAGTGTTC ATGTTGGAGC ACATTTTCCC ATTTTTAGAG ACATTCAATT CGCATCGATT 
    GACGTATTTG GCAGAGCACT GCTTCGTGAT GCTCTCGACA CTCAATACGA CTCGGAATCC 
    AACAAATTAC CGCCGAAAAT CAAGGAAATC CTTCCGCTTC AGGATCATCC GTTGTCCCAT 
    GTCACCCGAG CGTGCCGCAA AGTTTTCATG CCATTGGACC TATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294385.1
    CDS8..4615
    Translation

    Target ORF information:

    RefSeq Version NM_001307456.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307456.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGGTCT TCATCCCGGC ACCGGAGCCA TTTTTGAATC AGGAGACTGG CGAAGAGCTG 
    CAATTTGTCT ATGGGGAATT CCCGTCGAAA ATGCGAAGCG AGTACTTCCA ACTGGATCGA 
    CTTGAGAAAA ATGGGAAAGA TGGGGAAAAG GTTCCCATGC ATTTTGTTTC AGAAGCATCC 
    CGTTGCTCGG CAGTTCGCAC AAAACCGGAA ACCCGTTCCT CGTTGCTAGC CGCCACAAAT 
    GCTGTGAAAA CCAACCCGCT TCATTGGTCA AAAACGCTTC TGTTCTACTC ATATTCACTA 
    TGGTTCATGC AATTGGATAG CCTCCTCACC GCTGCTCCAA ACAAGAAGAA AATACTTCGT 
    CTGGCATTTA ATGTGCTCGA TCGAATGGAA AAAACCGAGA TTTTCCCGCT CGATCAAGTA 
    TGCTATCGAA TTCTTATCGA ACTTTGCGGA AAATATTCGG AGCCCGAGAT GGCTGTCAAG 
    GTTATGAGAG CCATGCAAAG AGCTGGGCTT GAACAGAACG CCGTCACCTA TGGAATCTAC 
    CACCGCGCCG TTATGGATGC CACGTGGCCG TCGCCAGCTC GCCAAAAAGC CATAAAAATG 
    TGGAATTTGC TGAGAATTGT GCTCACCGGC GCCGAAACTC TCCGAAAATG GGCGAATTTG 
    GAAAGAAGAC CGCTGAAAAT TGATGAAAAA TCGGTGGAAA ACACGGAAAA TGCTGAGAAT 
    GTGCAGGCGG AGATCACGGT GGATTATCAG ATTACCAGCT ATCCACTGGA TTCTGTGGAA 
    AATTCGCCGA AAATGACCCC AAAACGGCAC AATTTGGAGC AAAAAGAGCC GCTGATGGAT 
    CCACTGGGTG CTCTGGGCCC CCTGGAAGCT GAAAATTTGG AAGTTTCACG CGAAAAAACC 
    AAGGAACGAT CAAAATCGCT GGGAAAAGTT GGAATGTCGC CAGCGAGAGC GAAATTCTTT 
    GCCGATCACG CTACACTGCC ATTTTCCAAC GAAAATACGC CGAAATCCGA TAAAAAAGAG 
    GCAAAAAGTG GATGGTTTAA GGGAATTGCG AATAGTCCAA TGTTTAAAAT GATTAAAAGT 
    ACTACTTTTG ATTCTTCCAA ACAAAACTCT GCTGAATCAT CAAGTGAAAC TAGCTCAATA 
    ACCGGAGCTT CTCCATCATT CCATTCCGTA GTAAATCAAT TCAAAAAGGG ATACGATGAC 
    GTCATGCCGT CTCGATTCCG TCTCGGCGTT TCCACACTAT TGAACAAATC CGTCGGTGCA 
    ATGGGCTCTG GAATGATTTA TTCGGACGAT CGGGCTTCTG CGGAAAATCC GTTCTTTGTG 
    AATCCAAAAG ATCCGGCTTA TGTGCTAGAT TCGGGCGTAC CGAACTGTCT GCTGCGGGAG 
    GAATATTGGA TGCGTGAGGT GTATTTGCAG TTGAAACGGA GCCGTGAGCG GGTTGAAAAG 
    GGTCAGAAAA GCGAGGAAAA CGAGGAGATT TCCGATGGAA TAGCGGATTT CCCCCAATTC 
    TCCGCAAAAA CTCCAGGAAA ACTGGAGGTG ATCCTGTGCA CCGCCACCTA CTGCCCGTCA 
    TGCCGAACAA TGGTCTACGA TGAGGAAATC ATGGCCGGCT GGAAAGTTGA CGATCAGAAT 
    TTGAACACAA TATGCCCACA CTGCTCGGGA AGCGGAACGG CGACAGACGC CGTCTCCAAC 
    GAAACCGCAG CCATTCAGGA GAGCGTTTTC GCGCCGAGAC TCAAAATTCG AATGAGATGG 
    ATTGAGGAGA TGAAGAGTAG TTGGTATCAG CCTGGTGGAC TGCAGGAGAC ACCGATTTCG 
    ACTCCCGAAA AAACGACGTC CACTCCCTCG GATATCCAGC TTGAAGTTGC ATTTGTAAGC 
    CCATTGGTGC TCCGTCGTGA GCTTGAAACA CTCCTATCCA CTGATAAGGA CGCGATGAGG 
    AATGAATCGC TGAAAACGAC GCATCCGGTG GTTTTCTGGA ATTTGATATA CTATTTGAGA 
    AGACTCTCCC TTCCGAGCCA TTTATTCTCG TGGATTGCCG AACGTCATCA CATTCGTTGC 
    GTCTTTGATC TACCAACACA ACACGACGAA ACCCGTCTCC CATTGTACTT TTACAACCCG 
    AATCACGATG GAAACGCGCC AAGTTTCGGC GATTTTACCG AAAAAATCAC CCGAAAATTG 
    GAGGATGGCT CGGATTCGAC GGGCTATGCT ACATGGAAGA CGGTCACCGA CTCGGTGAAC 
    AAGAATTTGC TGTTTAAGGC AGTTCAGACA CTGGTCAACT CGTCGCGATT GGTGAAAAAT 
    GGAAGTGTTC ATGTTGGAGC ACATTTTCCC ATTTTTAGAG ACATTCAATT CGCATCGATT 
    GACGTATTTG GCAGAGCACT GCTTCGTGAT GCTCTCGACA CTCAATACGA CTCGGAATCC 
    AACAAATTAC CGCCGAAAAT CAAGGAAATC CTTCCGCTTC AGGATCATCC GTTGTCCCAT 
    GTCACCCGAG CGTGCCGCAA AGTTTTCATG CCATTGGACC TATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307457.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294386.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.
    Price & Availability↑
    CloneID OCa344511
    Clone ID Related Accession (Same CDS sequence) NM_001307457.1 , NM_001307457.2
    Accession Version NM_001307457.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    AGTACGATTA ACGCGTTAGC TGATACAAGG ACGTCTATGG TCTTCATCCC GGCACCGGAG 
    CCATTTTTGA ATCAGGAGAC TGGCGAAGAG CTGCAATTTG TCTATGGGGA ATTCCCGTCG 
    AAAATGCGAA GCGAGTACTT CCAACTGGAT CGACTTGAGA AAAATGGGAA AGATGGGGAA 
    AAGGTTCCCA TGCATTTTGT TTCAGAAGCA TCCCGTTGCT CGGCAGTTCG CACAAAACCG 
    GAAACCCGTT CCTCGTTGCT AGCCGCCACA AATGCTGTGA AAACCAACCC GCTTCATTGG 
    TCAAAAACGC TTCTGTTCTA CTCATATTCA CTATGGTTCA TGCAATTGGA TAGCCTCCTC 
    ACCGCTGCTC CAAACAAGAA GAAAATACTT CGTCTGGCAT TTAATGTGCT CGATCGAATG 
    GAAAAAACCG AGATTTTCCC GCTCGATCAA GTATGCTATC GAATTCTTAT CGAACTTTGC 
    GGAAAATATT CGGAGCCCGA GATGGCTGTC AAGGTTATGA GAGCCATGCA AAGAGCTGGG 
    CTTGAACAGA ACGCCGTCAC CTATGGAATC TACCACCGCG CCGTTATGGA TGCCACGTGG 
    CCGTCGCCAG CTCGCCAAAA AGCCATAAAA ATGTGGAATT TGCTGAGAAT TGTGCTCACC 
    GGCGCCGAAA CTCTCCGAAA ATGGGCGAAT TTGGAAAGAA GACCGCTGAA AATTGATGAA 
    AAATCGGTGG AAAACACGGA AAATGCTGAG AATGTGCAGG CGGAGATCAC GGTGGATTAT 
    CAGATTACCA GCTATCCACT GGATTCTGTG GAAAATTCGC CGAAAATGAC CCCAAAACGG 
    CACAATTTGG AGCAAAAAGA GCCGCTGATG GATCCACTGG GTGCTCTGGG CCCCCTGGAA 
    GCTGAAAATT TGGAAGTTTC ACGCGAAAAA ACCAAGGAAC GATCAAAATC GCTGGGAAAA 
    GTTGGAATGT CGCCAGCGAG AGCGAAATTC TTTGCCGATC ACGCTACACT GCCATTTTCC 
    AACGAAAATA CGCCGAAATC CGATAAAAAA GAGGCAAAAA GTGGATGGTT TAAGGGAATT 
    GCGAATAGTC CAATGTTTAA AATGATTAAA AGTACTACTT TTGATTCTTC CAAACAAAAC 
    TCTGCTGAAT CATCAAGTGA AACTAGCTCA ATAACCGGAG CTTCTCCATC ATTCCATTCC 
    GTAGTAAATC AATTCAAAAA GGGATACGAT GACGTCATGC CGTCTCGATT CCGTCTCGGC 
    GTTTCCACAC TATTGAACAA ATCCGTCGGT GCAATGGGCT CTGGAATGAT TTATTCGGAC 
    GATCGGGCTT CTGCGGAAAA TCCGTTCTTT GTGAATCCAA AAGATCCGGC TTATGTGCTA 
    GATTCGGGCG TACCGAACTG TCTGCTGCGG GAGGAATATT GGATGCGTGA GGTGTATTTG 
    CAGTTGAAAC GGAGCCGTGA GCGGGTTGAA AAGGGTCAGA AAAGCGAGGA AAACGAGGAG 
    ATTTCCGATG GAATAGCGGA TTTCCCCCAA TTCTCCGCAA AAACTCCAGG AAAACTGGAG 
    GTGATCCTGT GCACCGCCAC CTACTGCCCG TCATGCCGAA CAATGGTCTA CGATGAGGAA 
    ATCATGGCCG GCTGGAAAGT TGACGATCAG AATTTGAACA CAATATGCCC ACACTGCTCG 
    GGAAGCGGAA CGGCGACAGA CGCCGTCTCC AACGAAACCG CAGCCATTCA GGAGAGCGTT 
    TTCGCGCCGA GACTCAAAAT TCGAATGAGA TGGATTGAGG AGATGAAGAG TAGTTGGTAT 
    CAGCCTGGTG GACTGCAGGA GACACCGATT TCGACTCCCG AAAAAACGAC GTCCACTCCC 
    TCGGATATCC AGCTTGAAGT TGCATTTGTA AGCCCATTGG TGCTCCGTCG TGAGCTTGAA 
    ACACTCCTAT CCACTGATAA GGACGCGATG AGGAATGAAT CGCTGAAAAC GACGCATCCG 
    GTGGTTTTCT GGAATTTGAT ATACTATTTG AGAAGACTCT CCCTTCCGAG CCATTTATTC 
    TCGTGGATTG CCGAACGTCA TCACATTCGT TGCGTCTTTG ATCTACCAAC ACAACACGAC 
    GAAACCCGTC TCCCATTGTA CTTTTACAAC CCGAATCACG ATGGAAACGC GCCAAGTTTC 
    GGCGATTTTA CCGAAAAAAT CACCCGAAAA TTGGAGGATG GCTCGGATTC GACGGGCTAT 
    GCTACATGGA AGACGGTCAC CGACTCGGTG AACAAGAATT TGCTGTTTAA GGCAGTTCAG 
    ACACTGGTCA ACTCGTCGCG ATTGGTGAAA AATGGAAGTG TTCATGTTGG AGCACATTTT 
    CCCATTTTTA GAGACATTCA ATTCGCATCG ATTGACGTAT TTGGCAGAGC ACTGCTTCGT 
    GATGCTCTCG ACACTCAATA CGACTCGGAA TCCAACAAAT TACCGCCGAA AATCAAGGAA 
    ATCCTTCCGC TTCAGGATCA TCCGTTGTCC CATGTCACCC GAGCGTGCCG CAAAGTTTTC 
    ATGCCATTGG ACCTATTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294386.1
    CDS1..4641
    Translation

    Target ORF information:

    RefSeq Version NM_001307457.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307457.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    AGTACGATTA ACGCGTTAGC TGATACAAGG ACGTCTATGG TCTTCATCCC GGCACCGGAG 
    CCATTTTTGA ATCAGGAGAC TGGCGAAGAG CTGCAATTTG TCTATGGGGA ATTCCCGTCG 
    AAAATGCGAA GCGAGTACTT CCAACTGGAT CGACTTGAGA AAAATGGGAA AGATGGGGAA 
    AAGGTTCCCA TGCATTTTGT TTCAGAAGCA TCCCGTTGCT CGGCAGTTCG CACAAAACCG 
    GAAACCCGTT CCTCGTTGCT AGCCGCCACA AATGCTGTGA AAACCAACCC GCTTCATTGG 
    TCAAAAACGC TTCTGTTCTA CTCATATTCA CTATGGTTCA TGCAATTGGA TAGCCTCCTC 
    ACCGCTGCTC CAAACAAGAA GAAAATACTT CGTCTGGCAT TTAATGTGCT CGATCGAATG 
    GAAAAAACCG AGATTTTCCC GCTCGATCAA GTATGCTATC GAATTCTTAT CGAACTTTGC 
    GGAAAATATT CGGAGCCCGA GATGGCTGTC AAGGTTATGA GAGCCATGCA AAGAGCTGGG 
    CTTGAACAGA ACGCCGTCAC CTATGGAATC TACCACCGCG CCGTTATGGA TGCCACGTGG 
    CCGTCGCCAG CTCGCCAAAA AGCCATAAAA ATGTGGAATT TGCTGAGAAT TGTGCTCACC 
    GGCGCCGAAA CTCTCCGAAA ATGGGCGAAT TTGGAAAGAA GACCGCTGAA AATTGATGAA 
    AAATCGGTGG AAAACACGGA AAATGCTGAG AATGTGCAGG CGGAGATCAC GGTGGATTAT 
    CAGATTACCA GCTATCCACT GGATTCTGTG GAAAATTCGC CGAAAATGAC CCCAAAACGG 
    CACAATTTGG AGCAAAAAGA GCCGCTGATG GATCCACTGG GTGCTCTGGG CCCCCTGGAA 
    GCTGAAAATT TGGAAGTTTC ACGCGAAAAA ACCAAGGAAC GATCAAAATC GCTGGGAAAA 
    GTTGGAATGT CGCCAGCGAG AGCGAAATTC TTTGCCGATC ACGCTACACT GCCATTTTCC 
    AACGAAAATA CGCCGAAATC CGATAAAAAA GAGGCAAAAA GTGGATGGTT TAAGGGAATT 
    GCGAATAGTC CAATGTTTAA AATGATTAAA AGTACTACTT TTGATTCTTC CAAACAAAAC 
    TCTGCTGAAT CATCAAGTGA AACTAGCTCA ATAACCGGAG CTTCTCCATC ATTCCATTCC 
    GTAGTAAATC AATTCAAAAA GGGATACGAT GACGTCATGC CGTCTCGATT CCGTCTCGGC 
    GTTTCCACAC TATTGAACAA ATCCGTCGGT GCAATGGGCT CTGGAATGAT TTATTCGGAC 
    GATCGGGCTT CTGCGGAAAA TCCGTTCTTT GTGAATCCAA AAGATCCGGC TTATGTGCTA 
    GATTCGGGCG TACCGAACTG TCTGCTGCGG GAGGAATATT GGATGCGTGA GGTGTATTTG 
    CAGTTGAAAC GGAGCCGTGA GCGGGTTGAA AAGGGTCAGA AAAGCGAGGA AAACGAGGAG 
    ATTTCCGATG GAATAGCGGA TTTCCCCCAA TTCTCCGCAA AAACTCCAGG AAAACTGGAG 
    GTGATCCTGT GCACCGCCAC CTACTGCCCG TCATGCCGAA CAATGGTCTA CGATGAGGAA 
    ATCATGGCCG GCTGGAAAGT TGACGATCAG AATTTGAACA CAATATGCCC ACACTGCTCG 
    GGAAGCGGAA CGGCGACAGA CGCCGTCTCC AACGAAACCG CAGCCATTCA GGAGAGCGTT 
    TTCGCGCCGA GACTCAAAAT TCGAATGAGA TGGATTGAGG AGATGAAGAG TAGTTGGTAT 
    CAGCCTGGTG GACTGCAGGA GACACCGATT TCGACTCCCG AAAAAACGAC GTCCACTCCC 
    TCGGATATCC AGCTTGAAGT TGCATTTGTA AGCCCATTGG TGCTCCGTCG TGAGCTTGAA 
    ACACTCCTAT CCACTGATAA GGACGCGATG AGGAATGAAT CGCTGAAAAC GACGCATCCG 
    GTGGTTTTCT GGAATTTGAT ATACTATTTG AGAAGACTCT CCCTTCCGAG CCATTTATTC 
    TCGTGGATTG CCGAACGTCA TCACATTCGT TGCGTCTTTG ATCTACCAAC ACAACACGAC 
    GAAACCCGTC TCCCATTGTA CTTTTACAAC CCGAATCACG ATGGAAACGC GCCAAGTTTC 
    GGCGATTTTA CCGAAAAAAT CACCCGAAAA TTGGAGGATG GCTCGGATTC GACGGGCTAT 
    GCTACATGGA AGACGGTCAC CGACTCGGTG AACAAGAATT TGCTGTTTAA GGCAGTTCAG 
    ACACTGGTCA ACTCGTCGCG ATTGGTGAAA AATGGAAGTG TTCATGTTGG AGCACATTTT 
    CCCATTTTTA GAGACATTCA ATTCGCATCG ATTGACGTAT TTGGCAGAGC ACTGCTTCGT 
    GATGCTCTCG ACACTCAATA CGACTCGGAA TCCAACAAAT TACCGCCGAA AATCAAGGAA 
    ATCCTTCCGC TTCAGGATCA TCCGTTGTCC CATGTCACCC GAGCGTGCCG CAAAGTTTTC 
    ATGCCATTGG ACCTATTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307457.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294386.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.
    Price & Availability↑
    CloneID OCa344511
    Clone ID Related Accession (Same CDS sequence) NM_001307457.1 , NM_001307457.2
    Accession Version NM_001307457.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307457.1. COMPLETENESS: incomplete on the 5' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    AGTACGATTA ACGCGTTAGC TGATACAAGG ACGTCTATGG TCTTCATCCC GGCACCGGAG 
    CCATTTTTGA ATCAGGAGAC TGGCGAAGAG CTGCAATTTG TCTATGGGGA ATTCCCGTCG 
    AAAATGCGAA GCGAGTACTT CCAACTGGAT CGACTTGAGA AAAATGGGAA AGATGGGGAA 
    AAGGTTCCCA TGCATTTTGT TTCAGAAGCA TCCCGTTGCT CGGCAGTTCG CACAAAACCG 
    GAAACCCGTT CCTCGTTGCT AGCCGCCACA AATGCTGTGA AAACCAACCC GCTTCATTGG 
    TCAAAAACGC TTCTGTTCTA CTCATATTCA CTATGGTTCA TGCAATTGGA TAGCCTCCTC 
    ACCGCTGCTC CAAACAAGAA GAAAATACTT CGTCTGGCAT TTAATGTGCT CGATCGAATG 
    GAAAAAACCG AGATTTTCCC GCTCGATCAA GTATGCTATC GAATTCTTAT CGAACTTTGC 
    GGAAAATATT CGGAGCCCGA GATGGCTGTC AAGGTTATGA GAGCCATGCA AAGAGCTGGG 
    CTTGAACAGA ACGCCGTCAC CTATGGAATC TACCACCGCG CCGTTATGGA TGCCACGTGG 
    CCGTCGCCAG CTCGCCAAAA AGCCATAAAA ATGTGGAATT TGCTGAGAAT TGTGCTCACC 
    GGCGCCGAAA CTCTCCGAAA ATGGGCGAAT TTGGAAAGAA GACCGCTGAA AATTGATGAA 
    AAATCGGTGG AAAACACGGA AAATGCTGAG AATGTGCAGG CGGAGATCAC GGTGGATTAT 
    CAGATTACCA GCTATCCACT GGATTCTGTG GAAAATTCGC CGAAAATGAC CCCAAAACGG 
    CACAATTTGG AGCAAAAAGA GCCGCTGATG GATCCACTGG GTGCTCTGGG CCCCCTGGAA 
    GCTGAAAATT TGGAAGTTTC ACGCGAAAAA ACCAAGGAAC GATCAAAATC GCTGGGAAAA 
    GTTGGAATGT CGCCAGCGAG AGCGAAATTC TTTGCCGATC ACGCTACACT GCCATTTTCC 
    AACGAAAATA CGCCGAAATC CGATAAAAAA GAGGCAAAAA GTGGATGGTT TAAGGGAATT 
    GCGAATAGTC CAATGTTTAA AATGATTAAA AGTACTACTT TTGATTCTTC CAAACAAAAC 
    TCTGCTGAAT CATCAAGTGA AACTAGCTCA ATAACCGGAG CTTCTCCATC ATTCCATTCC 
    GTAGTAAATC AATTCAAAAA GGGATACGAT GACGTCATGC CGTCTCGATT CCGTCTCGGC 
    GTTTCCACAC TATTGAACAA ATCCGTCGGT GCAATGGGCT CTGGAATGAT TTATTCGGAC 
    GATCGGGCTT CTGCGGAAAA TCCGTTCTTT GTGAATCCAA AAGATCCGGC TTATGTGCTA 
    GATTCGGGCG TACCGAACTG TCTGCTGCGG GAGGAATATT GGATGCGTGA GGTGTATTTG 
    CAGTTGAAAC GGAGCCGTGA GCGGGTTGAA AAGGGTCAGA AAAGCGAGGA AAACGAGGAG 
    ATTTCCGATG GAATAGCGGA TTTCCCCCAA TTCTCCGCAA AAACTCCAGG AAAACTGGAG 
    GTGATCCTGT GCACCGCCAC CTACTGCCCG TCATGCCGAA CAATGGTCTA CGATGAGGAA 
    ATCATGGCCG GCTGGAAAGT TGACGATCAG AATTTGAACA CAATATGCCC ACACTGCTCG 
    GGAAGCGGAA CGGCGACAGA CGCCGTCTCC AACGAAACCG CAGCCATTCA GGAGAGCGTT 
    TTCGCGCCGA GACTCAAAAT TCGAATGAGA TGGATTGAGG AGATGAAGAG TAGTTGGTAT 
    CAGCCTGGTG GACTGCAGGA GACACCGATT TCGACTCCCG AAAAAACGAC GTCCACTCCC 
    TCGGATATCC AGCTTGAAGT TGCATTTGTA AGCCCATTGG TGCTCCGTCG TGAGCTTGAA 
    ACACTCCTAT CCACTGATAA GGACGCGATG AGGAATGAAT CGCTGAAAAC GACGCATCCG 
    GTGGTTTTCT GGAATTTGAT ATACTATTTG AGAAGACTCT CCCTTCCGAG CCATTTATTC 
    TCGTGGATTG CCGAACGTCA TCACATTCGT TGCGTCTTTG ATCTACCAAC ACAACACGAC 
    GAAACCCGTC TCCCATTGTA CTTTTACAAC CCGAATCACG ATGGAAACGC GCCAAGTTTC 
    GGCGATTTTA CCGAAAAAAT CACCCGAAAA TTGGAGGATG GCTCGGATTC GACGGGCTAT 
    GCTACATGGA AGACGGTCAC CGACTCGGTG AACAAGAATT TGCTGTTTAA GGCAGTTCAG 
    ACACTGGTCA ACTCGTCGCG ATTGGTGAAA AATGGAAGTG TTCATGTTGG AGCACATTTT 
    CCCATTTTTA GAGACATTCA ATTCGCATCG ATTGACGTAT TTGGCAGAGC ACTGCTTCGT 
    GATGCTCTCG ACACTCAATA CGACTCGGAA TCCAACAAAT TACCGCCGAA AATCAAGGAA 
    ATCCTTCCGC TTCAGGATCA TCCGTTGTCC CATGTCACCC GAGCGTGCCG CAAAGTTTTC 
    ATGCCATTGG ACCTATTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294386.1
    CDS1..4641
    Translation

    Target ORF information:

    RefSeq Version NM_001307457.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307457.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    AGTACGATTA ACGCGTTAGC TGATACAAGG ACGTCTATGG TCTTCATCCC GGCACCGGAG 
    CCATTTTTGA ATCAGGAGAC TGGCGAAGAG CTGCAATTTG TCTATGGGGA ATTCCCGTCG 
    AAAATGCGAA GCGAGTACTT CCAACTGGAT CGACTTGAGA AAAATGGGAA AGATGGGGAA 
    AAGGTTCCCA TGCATTTTGT TTCAGAAGCA TCCCGTTGCT CGGCAGTTCG CACAAAACCG 
    GAAACCCGTT CCTCGTTGCT AGCCGCCACA AATGCTGTGA AAACCAACCC GCTTCATTGG 
    TCAAAAACGC TTCTGTTCTA CTCATATTCA CTATGGTTCA TGCAATTGGA TAGCCTCCTC 
    ACCGCTGCTC CAAACAAGAA GAAAATACTT CGTCTGGCAT TTAATGTGCT CGATCGAATG 
    GAAAAAACCG AGATTTTCCC GCTCGATCAA GTATGCTATC GAATTCTTAT CGAACTTTGC 
    GGAAAATATT CGGAGCCCGA GATGGCTGTC AAGGTTATGA GAGCCATGCA AAGAGCTGGG 
    CTTGAACAGA ACGCCGTCAC CTATGGAATC TACCACCGCG CCGTTATGGA TGCCACGTGG 
    CCGTCGCCAG CTCGCCAAAA AGCCATAAAA ATGTGGAATT TGCTGAGAAT TGTGCTCACC 
    GGCGCCGAAA CTCTCCGAAA ATGGGCGAAT TTGGAAAGAA GACCGCTGAA AATTGATGAA 
    AAATCGGTGG AAAACACGGA AAATGCTGAG AATGTGCAGG CGGAGATCAC GGTGGATTAT 
    CAGATTACCA GCTATCCACT GGATTCTGTG GAAAATTCGC CGAAAATGAC CCCAAAACGG 
    CACAATTTGG AGCAAAAAGA GCCGCTGATG GATCCACTGG GTGCTCTGGG CCCCCTGGAA 
    GCTGAAAATT TGGAAGTTTC ACGCGAAAAA ACCAAGGAAC GATCAAAATC GCTGGGAAAA 
    GTTGGAATGT CGCCAGCGAG AGCGAAATTC TTTGCCGATC ACGCTACACT GCCATTTTCC 
    AACGAAAATA CGCCGAAATC CGATAAAAAA GAGGCAAAAA GTGGATGGTT TAAGGGAATT 
    GCGAATAGTC CAATGTTTAA AATGATTAAA AGTACTACTT TTGATTCTTC CAAACAAAAC 
    TCTGCTGAAT CATCAAGTGA AACTAGCTCA ATAACCGGAG CTTCTCCATC ATTCCATTCC 
    GTAGTAAATC AATTCAAAAA GGGATACGAT GACGTCATGC CGTCTCGATT CCGTCTCGGC 
    GTTTCCACAC TATTGAACAA ATCCGTCGGT GCAATGGGCT CTGGAATGAT TTATTCGGAC 
    GATCGGGCTT CTGCGGAAAA TCCGTTCTTT GTGAATCCAA AAGATCCGGC TTATGTGCTA 
    GATTCGGGCG TACCGAACTG TCTGCTGCGG GAGGAATATT GGATGCGTGA GGTGTATTTG 
    CAGTTGAAAC GGAGCCGTGA GCGGGTTGAA AAGGGTCAGA AAAGCGAGGA AAACGAGGAG 
    ATTTCCGATG GAATAGCGGA TTTCCCCCAA TTCTCCGCAA AAACTCCAGG AAAACTGGAG 
    GTGATCCTGT GCACCGCCAC CTACTGCCCG TCATGCCGAA CAATGGTCTA CGATGAGGAA 
    ATCATGGCCG GCTGGAAAGT TGACGATCAG AATTTGAACA CAATATGCCC ACACTGCTCG 
    GGAAGCGGAA CGGCGACAGA CGCCGTCTCC AACGAAACCG CAGCCATTCA GGAGAGCGTT 
    TTCGCGCCGA GACTCAAAAT TCGAATGAGA TGGATTGAGG AGATGAAGAG TAGTTGGTAT 
    CAGCCTGGTG GACTGCAGGA GACACCGATT TCGACTCCCG AAAAAACGAC GTCCACTCCC 
    TCGGATATCC AGCTTGAAGT TGCATTTGTA AGCCCATTGG TGCTCCGTCG TGAGCTTGAA 
    ACACTCCTAT CCACTGATAA GGACGCGATG AGGAATGAAT CGCTGAAAAC GACGCATCCG 
    GTGGTTTTCT GGAATTTGAT ATACTATTTG AGAAGACTCT CCCTTCCGAG CCATTTATTC 
    TCGTGGATTG CCGAACGTCA TCACATTCGT TGCGTCTTTG ATCTACCAAC ACAACACGAC 
    GAAACCCGTC TCCCATTGTA CTTTTACAAC CCGAATCACG ATGGAAACGC GCCAAGTTTC 
    GGCGATTTTA CCGAAAAAAT CACCCGAAAA TTGGAGGATG GCTCGGATTC GACGGGCTAT 
    GCTACATGGA AGACGGTCAC CGACTCGGTG AACAAGAATT TGCTGTTTAA GGCAGTTCAG 
    ACACTGGTCA ACTCGTCGCG ATTGGTGAAA AATGGAAGTG TTCATGTTGG AGCACATTTT 
    CCCATTTTTA GAGACATTCA ATTCGCATCG ATTGACGTAT TTGGCAGAGC ACTGCTTCGT 
    GATGCTCTCG ACACTCAATA CGACTCGGAA TCCAACAAAT TACCGCCGAA AATCAAGGAA 
    ATCCTTCCGC TTCAGGATCA TCCGTTGTCC CATGTCACCC GAGCGTGCCG CAAAGTTTTC 
    ATGCCATTGG ACCTATTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307458.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294387.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.
    Price & Availability↑
    CloneID OCa344512
    Clone ID Related Accession (Same CDS sequence) NM_001307458.1 , NM_001307458.2
    Accession Version NM_001307458.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4647bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGAGTA CGATTAACGC GTTAGCTGAT ACAAGGACGT CTATGGTCTT CATCCCGGCA 
    CCGGAGCCAT TTTTGAATCA GGAGACTGGC GAAGAGCTGC AATTTGTCTA TGGGGAATTC 
    CCGTCGAAAA TGCGAAGCGA GTACTTCCAA CTGGATCGAC TTGAGAAAAA TGGGAAAGAT 
    GGGGAAAAGG TTCCCATGCA TTTTGTTTCA GAAGCATCCC GTTGCTCGGC AGTTCGCACA 
    AAACCGGAAA CCCGTTCCTC GTTGCTAGCC GCCACAAATG CTGTGAAAAC CAACCCGCTT 
    CATTGGTCAA AAACGCTTCT GTTCTACTCA TATTCACTAT GGTTCATGCA ATTGGATAGC 
    CTCCTCACCG CTGCTCCAAA CAAGAAGAAA ATACTTCGTC TGGCATTTAA TGTGCTCGAT 
    CGAATGGAAA AAACCGAGAT TTTCCCGCTC GATCAAGTAT GCTATCGAAT TCTTATCGAA 
    CTTTGCGGAA AATATTCGGA GCCCGAGATG GCTGTCAAGG TTATGAGAGC CATGCAAAGA 
    GCTGGGCTTG AACAGAACGC CGTCACCTAT GGAATCTACC ACCGCGCCGT TATGGATGCC 
    ACGTGGCCGT CGCCAGCTCG CCAAAAAGCC ATAAAAATGT GGAATTTGCT GAGAATTGTG 
    CTCACCGGCG CCGAAACTCT CCGAAAATGG GCGAATTTGG AAAGAAGACC GCTGAAAATT 
    GATGAAAAAT CGGTGGAAAA CACGGAAAAT GCTGAGAATG TGCAGGCGGA GATCACGGTG 
    GATTATCAGA TTACCAGCTA TCCACTGGAT TCTGTGGAAA ATTCGCCGAA AATGACCCCA 
    AAACGGCACA ATTTGGAGCA AAAAGAGCCG CTGATGGATC CACTGGGTGC TCTGGGCCCC 
    CTGGAAGCTG AAAATTTGGA AGTTTCACGC GAAAAAACCA AGGAACGATC AAAATCGCTG 
    GGAAAAGTTG GAATGTCGCC AGCGAGAGCG AAATTCTTTG CCGATCACGC TACACTGCCA 
    TTTTCCAACG AAAATACGCC GAAATCCGAT AAAAAAGAGG CAAAAAGTGG ATGGTTTAAG 
    GGAATTGCGA ATAGTCCAAT GTTTAAAATG ATTAAAAGTA CTACTTTTGA TTCTTCCAAA 
    CAAAACTCTG CTGAATCATC AAGTGAAACT AGCTCAATAA CCGGAGCTTC TCCATCATTC 
    CATTCCGTAG TAAATCAATT CAAAAAGGGA TACGATGACG TCATGCCGTC TCGATTCCGT 
    CTCGGCGTTT CCACACTATT GAACAAATCC GTCGGTGCAA TGGGCTCTGG AATGATTTAT 
    TCGGACGATC GGGCTTCTGC GGAAAATCCG TTCTTTGTGA ATCCAAAAGA TCCGGCTTAT 
    GTGCTAGATT CGGGCGTACC GAACTGTCTG CTGCGGGAGG AATATTGGAT GCGTGAGGTG 
    TATTTGCAGT TGAAACGGAG CCGTGAGCGG GTTGAAAAGG GTCAGAAAAG CGAGGAAAAC 
    GAGGAGATTT CCGATGGAAT AGCGGATTTC CCCCAATTCT CCGCAAAAAC TCCAGGAAAA 
    CTGGAGGTGA TCCTGTGCAC CGCCACCTAC TGCCCGTCAT GCCGAACAAT GGTCTACGAT 
    GAGGAAATCA TGGCCGGCTG GAAAGTTGAC GATCAGAATT TGAACACAAT ATGCCCACAC 
    TGCTCGGGAA GCGGAACGGC GACAGACGCC GTCTCCAACG AAACCGCAGC CATTCAGGAG 
    AGCGTTTTCG CGCCGAGACT CAAAATTCGA ATGAGATGGA TTGAGGAGAT GAAGAGTAGT 
    TGGTATCAGC CTGGTGGACT GCAGGAGACA CCGATTTCGA CTCCCGAAAA AACGACGTCC 
    ACTCCCTCGG ATATCCAGCT TGAAGTTGCA TTTGTAAGCC CATTGGTGCT CCGTCGTGAG 
    CTTGAAACAC TCCTATCCAC TGATAAGGAC GCGATGAGGA ATGAATCGCT GAAAACGACG 
    CATCCGGTGG TTTTCTGGAA TTTGATATAC TATTTGAGAA GACTCTCCCT TCCGAGCCAT 
    TTATTCTCGT GGATTGCCGA ACGTCATCAC ATTCGTTGCG TCTTTGATCT ACCAACACAA 
    CACGACGAAA CCCGTCTCCC ATTGTACTTT TACAACCCGA ATCACGATGG AAACGCGCCA 
    AGTTTCGGCG ATTTTACCGA AAAAATCACC CGAAAATTGG AGGATGGCTC GGATTCGACG 
    GGCTATGCTA CATGGAAGAC GGTCACCGAC TCGGTGAACA AGAATTTGCT GTTTAAGGCA 
    GTTCAGACAC TGGTCAACTC GTCGCGATTG GTGAAAAATG GAAGTGTTCA TGTTGGAGCA 
    CATTTTCCCA TTTTTAGAGA CATTCAATTC GCATCGATTG ACGTATTTGG CAGAGCACTG 
    CTTCGTGATG CTCTCGACAC TCAATACGAC TCGGAATCCA ACAAATTACC GCCGAAAATC 
    AAGGAAATCC TTCCGCTTCA GGATCATCCG TTGTCCCATG TCACCCGAGC GTGCCGCAAA 
    GTTTTCATGC CATTGGACCT ATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294387.1
    CDS1..4647
    Translation

    Target ORF information:

    RefSeq Version NM_001307458.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307458.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGAGTA CGATTAACGC GTTAGCTGAT ACAAGGACGT CTATGGTCTT CATCCCGGCA 
    CCGGAGCCAT TTTTGAATCA GGAGACTGGC GAAGAGCTGC AATTTGTCTA TGGGGAATTC 
    CCGTCGAAAA TGCGAAGCGA GTACTTCCAA CTGGATCGAC TTGAGAAAAA TGGGAAAGAT 
    GGGGAAAAGG TTCCCATGCA TTTTGTTTCA GAAGCATCCC GTTGCTCGGC AGTTCGCACA 
    AAACCGGAAA CCCGTTCCTC GTTGCTAGCC GCCACAAATG CTGTGAAAAC CAACCCGCTT 
    CATTGGTCAA AAACGCTTCT GTTCTACTCA TATTCACTAT GGTTCATGCA ATTGGATAGC 
    CTCCTCACCG CTGCTCCAAA CAAGAAGAAA ATACTTCGTC TGGCATTTAA TGTGCTCGAT 
    CGAATGGAAA AAACCGAGAT TTTCCCGCTC GATCAAGTAT GCTATCGAAT TCTTATCGAA 
    CTTTGCGGAA AATATTCGGA GCCCGAGATG GCTGTCAAGG TTATGAGAGC CATGCAAAGA 
    GCTGGGCTTG AACAGAACGC CGTCACCTAT GGAATCTACC ACCGCGCCGT TATGGATGCC 
    ACGTGGCCGT CGCCAGCTCG CCAAAAAGCC ATAAAAATGT GGAATTTGCT GAGAATTGTG 
    CTCACCGGCG CCGAAACTCT CCGAAAATGG GCGAATTTGG AAAGAAGACC GCTGAAAATT 
    GATGAAAAAT CGGTGGAAAA CACGGAAAAT GCTGAGAATG TGCAGGCGGA GATCACGGTG 
    GATTATCAGA TTACCAGCTA TCCACTGGAT TCTGTGGAAA ATTCGCCGAA AATGACCCCA 
    AAACGGCACA ATTTGGAGCA AAAAGAGCCG CTGATGGATC CACTGGGTGC TCTGGGCCCC 
    CTGGAAGCTG AAAATTTGGA AGTTTCACGC GAAAAAACCA AGGAACGATC AAAATCGCTG 
    GGAAAAGTTG GAATGTCGCC AGCGAGAGCG AAATTCTTTG CCGATCACGC TACACTGCCA 
    TTTTCCAACG AAAATACGCC GAAATCCGAT AAAAAAGAGG CAAAAAGTGG ATGGTTTAAG 
    GGAATTGCGA ATAGTCCAAT GTTTAAAATG ATTAAAAGTA CTACTTTTGA TTCTTCCAAA 
    CAAAACTCTG CTGAATCATC AAGTGAAACT AGCTCAATAA CCGGAGCTTC TCCATCATTC 
    CATTCCGTAG TAAATCAATT CAAAAAGGGA TACGATGACG TCATGCCGTC TCGATTCCGT 
    CTCGGCGTTT CCACACTATT GAACAAATCC GTCGGTGCAA TGGGCTCTGG AATGATTTAT 
    TCGGACGATC GGGCTTCTGC GGAAAATCCG TTCTTTGTGA ATCCAAAAGA TCCGGCTTAT 
    GTGCTAGATT CGGGCGTACC GAACTGTCTG CTGCGGGAGG AATATTGGAT GCGTGAGGTG 
    TATTTGCAGT TGAAACGGAG CCGTGAGCGG GTTGAAAAGG GTCAGAAAAG CGAGGAAAAC 
    GAGGAGATTT CCGATGGAAT AGCGGATTTC CCCCAATTCT CCGCAAAAAC TCCAGGAAAA 
    CTGGAGGTGA TCCTGTGCAC CGCCACCTAC TGCCCGTCAT GCCGAACAAT GGTCTACGAT 
    GAGGAAATCA TGGCCGGCTG GAAAGTTGAC GATCAGAATT TGAACACAAT ATGCCCACAC 
    TGCTCGGGAA GCGGAACGGC GACAGACGCC GTCTCCAACG AAACCGCAGC CATTCAGGAG 
    AGCGTTTTCG CGCCGAGACT CAAAATTCGA ATGAGATGGA TTGAGGAGAT GAAGAGTAGT 
    TGGTATCAGC CTGGTGGACT GCAGGAGACA CCGATTTCGA CTCCCGAAAA AACGACGTCC 
    ACTCCCTCGG ATATCCAGCT TGAAGTTGCA TTTGTAAGCC CATTGGTGCT CCGTCGTGAG 
    CTTGAAACAC TCCTATCCAC TGATAAGGAC GCGATGAGGA ATGAATCGCT GAAAACGACG 
    CATCCGGTGG TTTTCTGGAA TTTGATATAC TATTTGAGAA GACTCTCCCT TCCGAGCCAT 
    TTATTCTCGT GGATTGCCGA ACGTCATCAC ATTCGTTGCG TCTTTGATCT ACCAACACAA 
    CACGACGAAA CCCGTCTCCC ATTGTACTTT TACAACCCGA ATCACGATGG AAACGCGCCA 
    AGTTTCGGCG ATTTTACCGA AAAAATCACC CGAAAATTGG AGGATGGCTC GGATTCGACG 
    GGCTATGCTA CATGGAAGAC GGTCACCGAC TCGGTGAACA AGAATTTGCT GTTTAAGGCA 
    GTTCAGACAC TGGTCAACTC GTCGCGATTG GTGAAAAATG GAAGTGTTCA TGTTGGAGCA 
    CATTTTCCCA TTTTTAGAGA CATTCAATTC GCATCGATTG ACGTATTTGG CAGAGCACTG 
    CTTCGTGATG CTCTCGACAC TCAATACGAC TCGGAATCCA ACAAATTACC GCCGAAAATC 
    AAGGAAATCC TTCCGCTTCA GGATCATCCG TTGTCCCATG TCACCCGAGC GTGCCGCAAA 
    GTTTTCATGC CATTGGACCT ATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307458.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294387.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.
    Price & Availability↑
    CloneID OCa344512
    Clone ID Related Accession (Same CDS sequence) NM_001307458.1 , NM_001307458.2
    Accession Version NM_001307458.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4647bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307458.1. COMPLETENESS: incomplete on the 5' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGAGTA CGATTAACGC GTTAGCTGAT ACAAGGACGT CTATGGTCTT CATCCCGGCA 
    CCGGAGCCAT TTTTGAATCA GGAGACTGGC GAAGAGCTGC AATTTGTCTA TGGGGAATTC 
    CCGTCGAAAA TGCGAAGCGA GTACTTCCAA CTGGATCGAC TTGAGAAAAA TGGGAAAGAT 
    GGGGAAAAGG TTCCCATGCA TTTTGTTTCA GAAGCATCCC GTTGCTCGGC AGTTCGCACA 
    AAACCGGAAA CCCGTTCCTC GTTGCTAGCC GCCACAAATG CTGTGAAAAC CAACCCGCTT 
    CATTGGTCAA AAACGCTTCT GTTCTACTCA TATTCACTAT GGTTCATGCA ATTGGATAGC 
    CTCCTCACCG CTGCTCCAAA CAAGAAGAAA ATACTTCGTC TGGCATTTAA TGTGCTCGAT 
    CGAATGGAAA AAACCGAGAT TTTCCCGCTC GATCAAGTAT GCTATCGAAT TCTTATCGAA 
    CTTTGCGGAA AATATTCGGA GCCCGAGATG GCTGTCAAGG TTATGAGAGC CATGCAAAGA 
    GCTGGGCTTG AACAGAACGC CGTCACCTAT GGAATCTACC ACCGCGCCGT TATGGATGCC 
    ACGTGGCCGT CGCCAGCTCG CCAAAAAGCC ATAAAAATGT GGAATTTGCT GAGAATTGTG 
    CTCACCGGCG CCGAAACTCT CCGAAAATGG GCGAATTTGG AAAGAAGACC GCTGAAAATT 
    GATGAAAAAT CGGTGGAAAA CACGGAAAAT GCTGAGAATG TGCAGGCGGA GATCACGGTG 
    GATTATCAGA TTACCAGCTA TCCACTGGAT TCTGTGGAAA ATTCGCCGAA AATGACCCCA 
    AAACGGCACA ATTTGGAGCA AAAAGAGCCG CTGATGGATC CACTGGGTGC TCTGGGCCCC 
    CTGGAAGCTG AAAATTTGGA AGTTTCACGC GAAAAAACCA AGGAACGATC AAAATCGCTG 
    GGAAAAGTTG GAATGTCGCC AGCGAGAGCG AAATTCTTTG CCGATCACGC TACACTGCCA 
    TTTTCCAACG AAAATACGCC GAAATCCGAT AAAAAAGAGG CAAAAAGTGG ATGGTTTAAG 
    GGAATTGCGA ATAGTCCAAT GTTTAAAATG ATTAAAAGTA CTACTTTTGA TTCTTCCAAA 
    CAAAACTCTG CTGAATCATC AAGTGAAACT AGCTCAATAA CCGGAGCTTC TCCATCATTC 
    CATTCCGTAG TAAATCAATT CAAAAAGGGA TACGATGACG TCATGCCGTC TCGATTCCGT 
    CTCGGCGTTT CCACACTATT GAACAAATCC GTCGGTGCAA TGGGCTCTGG AATGATTTAT 
    TCGGACGATC GGGCTTCTGC GGAAAATCCG TTCTTTGTGA ATCCAAAAGA TCCGGCTTAT 
    GTGCTAGATT CGGGCGTACC GAACTGTCTG CTGCGGGAGG AATATTGGAT GCGTGAGGTG 
    TATTTGCAGT TGAAACGGAG CCGTGAGCGG GTTGAAAAGG GTCAGAAAAG CGAGGAAAAC 
    GAGGAGATTT CCGATGGAAT AGCGGATTTC CCCCAATTCT CCGCAAAAAC TCCAGGAAAA 
    CTGGAGGTGA TCCTGTGCAC CGCCACCTAC TGCCCGTCAT GCCGAACAAT GGTCTACGAT 
    GAGGAAATCA TGGCCGGCTG GAAAGTTGAC GATCAGAATT TGAACACAAT ATGCCCACAC 
    TGCTCGGGAA GCGGAACGGC GACAGACGCC GTCTCCAACG AAACCGCAGC CATTCAGGAG 
    AGCGTTTTCG CGCCGAGACT CAAAATTCGA ATGAGATGGA TTGAGGAGAT GAAGAGTAGT 
    TGGTATCAGC CTGGTGGACT GCAGGAGACA CCGATTTCGA CTCCCGAAAA AACGACGTCC 
    ACTCCCTCGG ATATCCAGCT TGAAGTTGCA TTTGTAAGCC CATTGGTGCT CCGTCGTGAG 
    CTTGAAACAC TCCTATCCAC TGATAAGGAC GCGATGAGGA ATGAATCGCT GAAAACGACG 
    CATCCGGTGG TTTTCTGGAA TTTGATATAC TATTTGAGAA GACTCTCCCT TCCGAGCCAT 
    TTATTCTCGT GGATTGCCGA ACGTCATCAC ATTCGTTGCG TCTTTGATCT ACCAACACAA 
    CACGACGAAA CCCGTCTCCC ATTGTACTTT TACAACCCGA ATCACGATGG AAACGCGCCA 
    AGTTTCGGCG ATTTTACCGA AAAAATCACC CGAAAATTGG AGGATGGCTC GGATTCGACG 
    GGCTATGCTA CATGGAAGAC GGTCACCGAC TCGGTGAACA AGAATTTGCT GTTTAAGGCA 
    GTTCAGACAC TGGTCAACTC GTCGCGATTG GTGAAAAATG GAAGTGTTCA TGTTGGAGCA 
    CATTTTCCCA TTTTTAGAGA CATTCAATTC GCATCGATTG ACGTATTTGG CAGAGCACTG 
    CTTCGTGATG CTCTCGACAC TCAATACGAC TCGGAATCCA ACAAATTACC GCCGAAAATC 
    AAGGAAATCC TTCCGCTTCA GGATCATCCG TTGTCCCATG TCACCCGAGC GTGCCGCAAA 
    GTTTTCATGC CATTGGACCT ATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294387.1
    CDS1..4647
    Translation

    Target ORF information:

    RefSeq Version NM_001307458.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307458.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGAGTA CGATTAACGC GTTAGCTGAT ACAAGGACGT CTATGGTCTT CATCCCGGCA 
    CCGGAGCCAT TTTTGAATCA GGAGACTGGC GAAGAGCTGC AATTTGTCTA TGGGGAATTC 
    CCGTCGAAAA TGCGAAGCGA GTACTTCCAA CTGGATCGAC TTGAGAAAAA TGGGAAAGAT 
    GGGGAAAAGG TTCCCATGCA TTTTGTTTCA GAAGCATCCC GTTGCTCGGC AGTTCGCACA 
    AAACCGGAAA CCCGTTCCTC GTTGCTAGCC GCCACAAATG CTGTGAAAAC CAACCCGCTT 
    CATTGGTCAA AAACGCTTCT GTTCTACTCA TATTCACTAT GGTTCATGCA ATTGGATAGC 
    CTCCTCACCG CTGCTCCAAA CAAGAAGAAA ATACTTCGTC TGGCATTTAA TGTGCTCGAT 
    CGAATGGAAA AAACCGAGAT TTTCCCGCTC GATCAAGTAT GCTATCGAAT TCTTATCGAA 
    CTTTGCGGAA AATATTCGGA GCCCGAGATG GCTGTCAAGG TTATGAGAGC CATGCAAAGA 
    GCTGGGCTTG AACAGAACGC CGTCACCTAT GGAATCTACC ACCGCGCCGT TATGGATGCC 
    ACGTGGCCGT CGCCAGCTCG CCAAAAAGCC ATAAAAATGT GGAATTTGCT GAGAATTGTG 
    CTCACCGGCG CCGAAACTCT CCGAAAATGG GCGAATTTGG AAAGAAGACC GCTGAAAATT 
    GATGAAAAAT CGGTGGAAAA CACGGAAAAT GCTGAGAATG TGCAGGCGGA GATCACGGTG 
    GATTATCAGA TTACCAGCTA TCCACTGGAT TCTGTGGAAA ATTCGCCGAA AATGACCCCA 
    AAACGGCACA ATTTGGAGCA AAAAGAGCCG CTGATGGATC CACTGGGTGC TCTGGGCCCC 
    CTGGAAGCTG AAAATTTGGA AGTTTCACGC GAAAAAACCA AGGAACGATC AAAATCGCTG 
    GGAAAAGTTG GAATGTCGCC AGCGAGAGCG AAATTCTTTG CCGATCACGC TACACTGCCA 
    TTTTCCAACG AAAATACGCC GAAATCCGAT AAAAAAGAGG CAAAAAGTGG ATGGTTTAAG 
    GGAATTGCGA ATAGTCCAAT GTTTAAAATG ATTAAAAGTA CTACTTTTGA TTCTTCCAAA 
    CAAAACTCTG CTGAATCATC AAGTGAAACT AGCTCAATAA CCGGAGCTTC TCCATCATTC 
    CATTCCGTAG TAAATCAATT CAAAAAGGGA TACGATGACG TCATGCCGTC TCGATTCCGT 
    CTCGGCGTTT CCACACTATT GAACAAATCC GTCGGTGCAA TGGGCTCTGG AATGATTTAT 
    TCGGACGATC GGGCTTCTGC GGAAAATCCG TTCTTTGTGA ATCCAAAAGA TCCGGCTTAT 
    GTGCTAGATT CGGGCGTACC GAACTGTCTG CTGCGGGAGG AATATTGGAT GCGTGAGGTG 
    TATTTGCAGT TGAAACGGAG CCGTGAGCGG GTTGAAAAGG GTCAGAAAAG CGAGGAAAAC 
    GAGGAGATTT CCGATGGAAT AGCGGATTTC CCCCAATTCT CCGCAAAAAC TCCAGGAAAA 
    CTGGAGGTGA TCCTGTGCAC CGCCACCTAC TGCCCGTCAT GCCGAACAAT GGTCTACGAT 
    GAGGAAATCA TGGCCGGCTG GAAAGTTGAC GATCAGAATT TGAACACAAT ATGCCCACAC 
    TGCTCGGGAA GCGGAACGGC GACAGACGCC GTCTCCAACG AAACCGCAGC CATTCAGGAG 
    AGCGTTTTCG CGCCGAGACT CAAAATTCGA ATGAGATGGA TTGAGGAGAT GAAGAGTAGT 
    TGGTATCAGC CTGGTGGACT GCAGGAGACA CCGATTTCGA CTCCCGAAAA AACGACGTCC 
    ACTCCCTCGG ATATCCAGCT TGAAGTTGCA TTTGTAAGCC CATTGGTGCT CCGTCGTGAG 
    CTTGAAACAC TCCTATCCAC TGATAAGGAC GCGATGAGGA ATGAATCGCT GAAAACGACG 
    CATCCGGTGG TTTTCTGGAA TTTGATATAC TATTTGAGAA GACTCTCCCT TCCGAGCCAT 
    TTATTCTCGT GGATTGCCGA ACGTCATCAC ATTCGTTGCG TCTTTGATCT ACCAACACAA 
    CACGACGAAA CCCGTCTCCC ATTGTACTTT TACAACCCGA ATCACGATGG AAACGCGCCA 
    AGTTTCGGCG ATTTTACCGA AAAAATCACC CGAAAATTGG AGGATGGCTC GGATTCGACG 
    GGCTATGCTA CATGGAAGAC GGTCACCGAC TCGGTGAACA AGAATTTGCT GTTTAAGGCA 
    GTTCAGACAC TGGTCAACTC GTCGCGATTG GTGAAAAATG GAAGTGTTCA TGTTGGAGCA 
    CATTTTCCCA TTTTTAGAGA CATTCAATTC GCATCGATTG ACGTATTTGG CAGAGCACTG 
    CTTCGTGATG CTCTCGACAC TCAATACGAC TCGGAATCCA ACAAATTACC GCCGAAAATC 
    AAGGAAATCC TTCCGCTTCA GGATCATCCG TTGTCCCATG TCACCCGAGC GTGCCGCAAA 
    GTTTTCATGC CATTGGACCT ATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307459.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294388.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.
    Price & Availability↑
    CloneID OCa344513
    Clone ID Related Accession (Same CDS sequence) NM_001307459.1 , NM_001307459.2
    Accession Version NM_001307459.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    GTCTTCATCC CGGCACCGGA GCCATTTTTG AATCAGGAGA CTGGCGAAGA GCTGCAATTT 
    GTCTATGGGG AATTCCCGTC GAAAATGCGA AGCGAGTACT TCCAACTGGA TCGACTTGAG 
    AAAAATGGGA AAGATGGGGA AAAGGTTCCC ATGCATTTTG AAGCATCCCG TTGCTCGGCA 
    GTTCGCACAA AACCGGAAAC CCGTTCCTCG TTGCTAGCCG CCACAAATGC TGTGAAAACC 
    AACCCGCTTC ATTGGTCAAA AACGCTTCTG TTCTACTCAT ATTCACTATG GTTCATGCAA 
    TTGGATAGCC TCCTCACCGC TGCTCCAAAC AAGAAGAAAA TACTTCGTCT GGCATTTAAT 
    GTGCTCGATC GAATGGAAAA AACCGAGATT TTCCCGCTCG ATCAAGTATG CTATCGAATT 
    CTTATCGAAC TTTGCGGAAA ATATTCGGAG CCCGAGATGG CTGTCAAGGT TATGAGAGCC 
    ATGCAAAGAG CTGGGCTTGA ACAGAACGCC GTCACCTATG GAATCTACCA CCGCGCCGTT 
    ATGGATGCCA CGTGGCCGTC GCCAGCTCGC CAAAAAGCCA TAAAAATGTG GAATTTGCTG 
    AGAATTGTGC TCACCGGCGC CGAAACTCTC CGAAAATGGG CGAATTTGGA AAGAAGACCG 
    CTGAAAATTG ATGAAAAATC GGTGGAAAAC ACGGAAAATG CTGAGAATGT GCAGGCGGAG 
    ATCACGGTGG ATTATCAGAT TACCAGCTAT CCACTGGATT CTGTGGAAAA TTCGCCGAAA 
    ATGACCCCAA AACGGCACAA TTTGGAGCAA AAAGAGCCGC TGATGGATCC ACTGGGTGCT 
    CTGGGCCCCC TGGAAGCTGA AAATTTGGAA GTTTCACGCG AAAAAACCAA GGAACGATCA 
    AAATCGCTGG GAAAAGTTGG AATGTCGCCA GCGAGAGCGA AATTCTTTGC CGATCACGCT 
    ACACTGCCAT TTTCCAACGA AAATACGCCG AAATCCGATA AAAAAGAGGC AAAAAGTGGA 
    TGGTTTAAGG GAATTGCGAA TAGTCCAATG TTTAAAATGA TTAAAAGTAC TACTTTTGAT 
    TCTTCCAAAC AAAACTCTGC TGAATCATCA AGTGAAACTA GCTCAATAAC CGGAGCTTCT 
    CCATCATTCC ATTCCGTAGT AAATCAATTC AAAAAGGGAT ACGATGACGT CATGCCGTCT 
    CGATTCCGTC TCGGCGTTTC CACACTATTG AACAAATCCG TCGGTGCAAT GGGCTCTGGA 
    ATGATTTATT CGGACGATCG GGCTTCTGCG GAAAATCCGT TCTTTGTGAA TCCAAAAGAT 
    CCGGCTTATG TGCTAGATTC GGGCGTACCG AACTGTCTGC TGCGGGAGGA ATATTGGATG 
    CGTGAGGTGT ATTTGCAGTT GAAACGGAGC CGTGAGCGGG TTGAAAAGGG TCAGAAAAGC 
    GAGGAAAACG AGGAGATTTC CGATGGAATA GCGGATTTCC CCCAATTCTC CGCAAAAACT 
    CCAGGAAAAC TGGAGGTGAT CCTGTGCACC GCCACCTACT GCCCGTCATG CCGAACAATG 
    GTCTACGATG AGGAAATCAT GGCCGGCTGG AAAGTTGACG ATCAGAATTT GAACACAATA 
    TGCCCACACT GCTCGGGAAG CGGAACGGCG ACAGACGCCG TCTCCAACGA AACCGCAGCC 
    ATTCAGGAGA GCGTTTTCGC GCCGAGACTC AAAATTCGAA TGAGATGGAT TGAGGAGATG 
    AAGAGTAGTT GGTATCAGCC TGGTGGACTG CAGGAGACAC CGATTTCGAC TCCCGAAAAA 
    ACGACGTCCA CTCCCTCGGA TATCCAGCTT GAAGTTGCAT TTGTAAGCCC ATTGGTGCTC 
    CGTCGTGAGC TTGAAACACT CCTATCCACT GATAAGGACG CGATGAGGAA TGAATCGCTG 
    AAAACGACGC ATCCGGTGGT TTTCTGGAAT TTGATATACT ATTTGAGAAG ACTCTCCCTT 
    CCGAGCCATT TATTCTCGTG GATTGCCGAA CGTCATCACA TTCGTTGCGT CTTTGATCTA 
    CCAACACAAC ACGACGAAAC CCGTCTCCCA TTGTACTTTT ACAACCCGAA TCACGATGGA 
    AACGCGCCAA GTTTCGGCGA TTTTACCGAA AAAATCACCC GAAAATTGGA GGATGGCTCG 
    GATTCGACGG GCTATGCTAC ATGGAAGACG GTCACCGACT CGGTGAACAA GAATTTGCTG 
    TTTAAGGCAG TTCAGACACT GGTCAACTCG TCGCGATTGG TGAAAAATGG AAGTGTTCAT 
    GTTGGAGCAC ATTTTCCCAT TTTTAGAGAC ATTCAATTCG CATCGATTGA CGTATTTGGC 
    AGAGCACTGC TTCGTGATGC TCTCGACACT CAATACGACT CGGAATCCAA CAAATTACCG 
    CCGAAAATCA AGGAAATCCT TCCGCTTCAG GATCATCCGT TGTCCCATGT CACCCGAGCG 
    TGCCGCAAAG TTTTCATGCC ATTGGACCTA TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294388.1
    CDS1..4596
    Translation

    Target ORF information:

    RefSeq Version NM_001307459.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307459.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    GTCTTCATCC CGGCACCGGA GCCATTTTTG AATCAGGAGA CTGGCGAAGA GCTGCAATTT 
    GTCTATGGGG AATTCCCGTC GAAAATGCGA AGCGAGTACT TCCAACTGGA TCGACTTGAG 
    AAAAATGGGA AAGATGGGGA AAAGGTTCCC ATGCATTTTG AAGCATCCCG TTGCTCGGCA 
    GTTCGCACAA AACCGGAAAC CCGTTCCTCG TTGCTAGCCG CCACAAATGC TGTGAAAACC 
    AACCCGCTTC ATTGGTCAAA AACGCTTCTG TTCTACTCAT ATTCACTATG GTTCATGCAA 
    TTGGATAGCC TCCTCACCGC TGCTCCAAAC AAGAAGAAAA TACTTCGTCT GGCATTTAAT 
    GTGCTCGATC GAATGGAAAA AACCGAGATT TTCCCGCTCG ATCAAGTATG CTATCGAATT 
    CTTATCGAAC TTTGCGGAAA ATATTCGGAG CCCGAGATGG CTGTCAAGGT TATGAGAGCC 
    ATGCAAAGAG CTGGGCTTGA ACAGAACGCC GTCACCTATG GAATCTACCA CCGCGCCGTT 
    ATGGATGCCA CGTGGCCGTC GCCAGCTCGC CAAAAAGCCA TAAAAATGTG GAATTTGCTG 
    AGAATTGTGC TCACCGGCGC CGAAACTCTC CGAAAATGGG CGAATTTGGA AAGAAGACCG 
    CTGAAAATTG ATGAAAAATC GGTGGAAAAC ACGGAAAATG CTGAGAATGT GCAGGCGGAG 
    ATCACGGTGG ATTATCAGAT TACCAGCTAT CCACTGGATT CTGTGGAAAA TTCGCCGAAA 
    ATGACCCCAA AACGGCACAA TTTGGAGCAA AAAGAGCCGC TGATGGATCC ACTGGGTGCT 
    CTGGGCCCCC TGGAAGCTGA AAATTTGGAA GTTTCACGCG AAAAAACCAA GGAACGATCA 
    AAATCGCTGG GAAAAGTTGG AATGTCGCCA GCGAGAGCGA AATTCTTTGC CGATCACGCT 
    ACACTGCCAT TTTCCAACGA AAATACGCCG AAATCCGATA AAAAAGAGGC AAAAAGTGGA 
    TGGTTTAAGG GAATTGCGAA TAGTCCAATG TTTAAAATGA TTAAAAGTAC TACTTTTGAT 
    TCTTCCAAAC AAAACTCTGC TGAATCATCA AGTGAAACTA GCTCAATAAC CGGAGCTTCT 
    CCATCATTCC ATTCCGTAGT AAATCAATTC AAAAAGGGAT ACGATGACGT CATGCCGTCT 
    CGATTCCGTC TCGGCGTTTC CACACTATTG AACAAATCCG TCGGTGCAAT GGGCTCTGGA 
    ATGATTTATT CGGACGATCG GGCTTCTGCG GAAAATCCGT TCTTTGTGAA TCCAAAAGAT 
    CCGGCTTATG TGCTAGATTC GGGCGTACCG AACTGTCTGC TGCGGGAGGA ATATTGGATG 
    CGTGAGGTGT ATTTGCAGTT GAAACGGAGC CGTGAGCGGG TTGAAAAGGG TCAGAAAAGC 
    GAGGAAAACG AGGAGATTTC CGATGGAATA GCGGATTTCC CCCAATTCTC CGCAAAAACT 
    CCAGGAAAAC TGGAGGTGAT CCTGTGCACC GCCACCTACT GCCCGTCATG CCGAACAATG 
    GTCTACGATG AGGAAATCAT GGCCGGCTGG AAAGTTGACG ATCAGAATTT GAACACAATA 
    TGCCCACACT GCTCGGGAAG CGGAACGGCG ACAGACGCCG TCTCCAACGA AACCGCAGCC 
    ATTCAGGAGA GCGTTTTCGC GCCGAGACTC AAAATTCGAA TGAGATGGAT TGAGGAGATG 
    AAGAGTAGTT GGTATCAGCC TGGTGGACTG CAGGAGACAC CGATTTCGAC TCCCGAAAAA 
    ACGACGTCCA CTCCCTCGGA TATCCAGCTT GAAGTTGCAT TTGTAAGCCC ATTGGTGCTC 
    CGTCGTGAGC TTGAAACACT CCTATCCACT GATAAGGACG CGATGAGGAA TGAATCGCTG 
    AAAACGACGC ATCCGGTGGT TTTCTGGAAT TTGATATACT ATTTGAGAAG ACTCTCCCTT 
    CCGAGCCATT TATTCTCGTG GATTGCCGAA CGTCATCACA TTCGTTGCGT CTTTGATCTA 
    CCAACACAAC ACGACGAAAC CCGTCTCCCA TTGTACTTTT ACAACCCGAA TCACGATGGA 
    AACGCGCCAA GTTTCGGCGA TTTTACCGAA AAAATCACCC GAAAATTGGA GGATGGCTCG 
    GATTCGACGG GCTATGCTAC ATGGAAGACG GTCACCGACT CGGTGAACAA GAATTTGCTG 
    TTTAAGGCAG TTCAGACACT GGTCAACTCG TCGCGATTGG TGAAAAATGG AAGTGTTCAT 
    GTTGGAGCAC ATTTTCCCAT TTTTAGAGAC ATTCAATTCG CATCGATTGA CGTATTTGGC 
    AGAGCACTGC TTCGTGATGC TCTCGACACT CAATACGACT CGGAATCCAA CAAATTACCG 
    CCGAAAATCA AGGAAATCCT TCCGCTTCAG GATCATCCGT TGTCCCATGT CACCCGAGCG 
    TGCCGCAAAG TTTTCATGCC ATTGGACCTA TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307459.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294388.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA.
    Price & Availability↑
    CloneID OCa344513
    Clone ID Related Accession (Same CDS sequence) NM_001307459.1 , NM_001307459.2
    Accession Version NM_001307459.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307459.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    GTCTTCATCC CGGCACCGGA GCCATTTTTG AATCAGGAGA CTGGCGAAGA GCTGCAATTT 
    GTCTATGGGG AATTCCCGTC GAAAATGCGA AGCGAGTACT TCCAACTGGA TCGACTTGAG 
    AAAAATGGGA AAGATGGGGA AAAGGTTCCC ATGCATTTTG AAGCATCCCG TTGCTCGGCA 
    GTTCGCACAA AACCGGAAAC CCGTTCCTCG TTGCTAGCCG CCACAAATGC TGTGAAAACC 
    AACCCGCTTC ATTGGTCAAA AACGCTTCTG TTCTACTCAT ATTCACTATG GTTCATGCAA 
    TTGGATAGCC TCCTCACCGC TGCTCCAAAC AAGAAGAAAA TACTTCGTCT GGCATTTAAT 
    GTGCTCGATC GAATGGAAAA AACCGAGATT TTCCCGCTCG ATCAAGTATG CTATCGAATT 
    CTTATCGAAC TTTGCGGAAA ATATTCGGAG CCCGAGATGG CTGTCAAGGT TATGAGAGCC 
    ATGCAAAGAG CTGGGCTTGA ACAGAACGCC GTCACCTATG GAATCTACCA CCGCGCCGTT 
    ATGGATGCCA CGTGGCCGTC GCCAGCTCGC CAAAAAGCCA TAAAAATGTG GAATTTGCTG 
    AGAATTGTGC TCACCGGCGC CGAAACTCTC CGAAAATGGG CGAATTTGGA AAGAAGACCG 
    CTGAAAATTG ATGAAAAATC GGTGGAAAAC ACGGAAAATG CTGAGAATGT GCAGGCGGAG 
    ATCACGGTGG ATTATCAGAT TACCAGCTAT CCACTGGATT CTGTGGAAAA TTCGCCGAAA 
    ATGACCCCAA AACGGCACAA TTTGGAGCAA AAAGAGCCGC TGATGGATCC ACTGGGTGCT 
    CTGGGCCCCC TGGAAGCTGA AAATTTGGAA GTTTCACGCG AAAAAACCAA GGAACGATCA 
    AAATCGCTGG GAAAAGTTGG AATGTCGCCA GCGAGAGCGA AATTCTTTGC CGATCACGCT 
    ACACTGCCAT TTTCCAACGA AAATACGCCG AAATCCGATA AAAAAGAGGC AAAAAGTGGA 
    TGGTTTAAGG GAATTGCGAA TAGTCCAATG TTTAAAATGA TTAAAAGTAC TACTTTTGAT 
    TCTTCCAAAC AAAACTCTGC TGAATCATCA AGTGAAACTA GCTCAATAAC CGGAGCTTCT 
    CCATCATTCC ATTCCGTAGT AAATCAATTC AAAAAGGGAT ACGATGACGT CATGCCGTCT 
    CGATTCCGTC TCGGCGTTTC CACACTATTG AACAAATCCG TCGGTGCAAT GGGCTCTGGA 
    ATGATTTATT CGGACGATCG GGCTTCTGCG GAAAATCCGT TCTTTGTGAA TCCAAAAGAT 
    CCGGCTTATG TGCTAGATTC GGGCGTACCG AACTGTCTGC TGCGGGAGGA ATATTGGATG 
    CGTGAGGTGT ATTTGCAGTT GAAACGGAGC CGTGAGCGGG TTGAAAAGGG TCAGAAAAGC 
    GAGGAAAACG AGGAGATTTC CGATGGAATA GCGGATTTCC CCCAATTCTC CGCAAAAACT 
    CCAGGAAAAC TGGAGGTGAT CCTGTGCACC GCCACCTACT GCCCGTCATG CCGAACAATG 
    GTCTACGATG AGGAAATCAT GGCCGGCTGG AAAGTTGACG ATCAGAATTT GAACACAATA 
    TGCCCACACT GCTCGGGAAG CGGAACGGCG ACAGACGCCG TCTCCAACGA AACCGCAGCC 
    ATTCAGGAGA GCGTTTTCGC GCCGAGACTC AAAATTCGAA TGAGATGGAT TGAGGAGATG 
    AAGAGTAGTT GGTATCAGCC TGGTGGACTG CAGGAGACAC CGATTTCGAC TCCCGAAAAA 
    ACGACGTCCA CTCCCTCGGA TATCCAGCTT GAAGTTGCAT TTGTAAGCCC ATTGGTGCTC 
    CGTCGTGAGC TTGAAACACT CCTATCCACT GATAAGGACG CGATGAGGAA TGAATCGCTG 
    AAAACGACGC ATCCGGTGGT TTTCTGGAAT TTGATATACT ATTTGAGAAG ACTCTCCCTT 
    CCGAGCCATT TATTCTCGTG GATTGCCGAA CGTCATCACA TTCGTTGCGT CTTTGATCTA 
    CCAACACAAC ACGACGAAAC CCGTCTCCCA TTGTACTTTT ACAACCCGAA TCACGATGGA 
    AACGCGCCAA GTTTCGGCGA TTTTACCGAA AAAATCACCC GAAAATTGGA GGATGGCTCG 
    GATTCGACGG GCTATGCTAC ATGGAAGACG GTCACCGACT CGGTGAACAA GAATTTGCTG 
    TTTAAGGCAG TTCAGACACT GGTCAACTCG TCGCGATTGG TGAAAAATGG AAGTGTTCAT 
    GTTGGAGCAC ATTTTCCCAT TTTTAGAGAC ATTCAATTCG CATCGATTGA CGTATTTGGC 
    AGAGCACTGC TTCGTGATGC TCTCGACACT CAATACGACT CGGAATCCAA CAAATTACCG 
    CCGAAAATCA AGGAAATCCT TCCGCTTCAG GATCATCCGT TGTCCCATGT CACCCGAGCG 
    TGCCGCAAAG TTTTCATGCC ATTGGACCTA TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294388.1
    CDS8..4603
    Translation

    Target ORF information:

    RefSeq Version NM_001307459.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307459.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    GTCTTCATCC CGGCACCGGA GCCATTTTTG AATCAGGAGA CTGGCGAAGA GCTGCAATTT 
    GTCTATGGGG AATTCCCGTC GAAAATGCGA AGCGAGTACT TCCAACTGGA TCGACTTGAG 
    AAAAATGGGA AAGATGGGGA AAAGGTTCCC ATGCATTTTG AAGCATCCCG TTGCTCGGCA 
    GTTCGCACAA AACCGGAAAC CCGTTCCTCG TTGCTAGCCG CCACAAATGC TGTGAAAACC 
    AACCCGCTTC ATTGGTCAAA AACGCTTCTG TTCTACTCAT ATTCACTATG GTTCATGCAA 
    TTGGATAGCC TCCTCACCGC TGCTCCAAAC AAGAAGAAAA TACTTCGTCT GGCATTTAAT 
    GTGCTCGATC GAATGGAAAA AACCGAGATT TTCCCGCTCG ATCAAGTATG CTATCGAATT 
    CTTATCGAAC TTTGCGGAAA ATATTCGGAG CCCGAGATGG CTGTCAAGGT TATGAGAGCC 
    ATGCAAAGAG CTGGGCTTGA ACAGAACGCC GTCACCTATG GAATCTACCA CCGCGCCGTT 
    ATGGATGCCA CGTGGCCGTC GCCAGCTCGC CAAAAAGCCA TAAAAATGTG GAATTTGCTG 
    AGAATTGTGC TCACCGGCGC CGAAACTCTC CGAAAATGGG CGAATTTGGA AAGAAGACCG 
    CTGAAAATTG ATGAAAAATC GGTGGAAAAC ACGGAAAATG CTGAGAATGT GCAGGCGGAG 
    ATCACGGTGG ATTATCAGAT TACCAGCTAT CCACTGGATT CTGTGGAAAA TTCGCCGAAA 
    ATGACCCCAA AACGGCACAA TTTGGAGCAA AAAGAGCCGC TGATGGATCC ACTGGGTGCT 
    CTGGGCCCCC TGGAAGCTGA AAATTTGGAA GTTTCACGCG AAAAAACCAA GGAACGATCA 
    AAATCGCTGG GAAAAGTTGG AATGTCGCCA GCGAGAGCGA AATTCTTTGC CGATCACGCT 
    ACACTGCCAT TTTCCAACGA AAATACGCCG AAATCCGATA AAAAAGAGGC AAAAAGTGGA 
    TGGTTTAAGG GAATTGCGAA TAGTCCAATG TTTAAAATGA TTAAAAGTAC TACTTTTGAT 
    TCTTCCAAAC AAAACTCTGC TGAATCATCA AGTGAAACTA GCTCAATAAC CGGAGCTTCT 
    CCATCATTCC ATTCCGTAGT AAATCAATTC AAAAAGGGAT ACGATGACGT CATGCCGTCT 
    CGATTCCGTC TCGGCGTTTC CACACTATTG AACAAATCCG TCGGTGCAAT GGGCTCTGGA 
    ATGATTTATT CGGACGATCG GGCTTCTGCG GAAAATCCGT TCTTTGTGAA TCCAAAAGAT 
    CCGGCTTATG TGCTAGATTC GGGCGTACCG AACTGTCTGC TGCGGGAGGA ATATTGGATG 
    CGTGAGGTGT ATTTGCAGTT GAAACGGAGC CGTGAGCGGG TTGAAAAGGG TCAGAAAAGC 
    GAGGAAAACG AGGAGATTTC CGATGGAATA GCGGATTTCC CCCAATTCTC CGCAAAAACT 
    CCAGGAAAAC TGGAGGTGAT CCTGTGCACC GCCACCTACT GCCCGTCATG CCGAACAATG 
    GTCTACGATG AGGAAATCAT GGCCGGCTGG AAAGTTGACG ATCAGAATTT GAACACAATA 
    TGCCCACACT GCTCGGGAAG CGGAACGGCG ACAGACGCCG TCTCCAACGA AACCGCAGCC 
    ATTCAGGAGA GCGTTTTCGC GCCGAGACTC AAAATTCGAA TGAGATGGAT TGAGGAGATG 
    AAGAGTAGTT GGTATCAGCC TGGTGGACTG CAGGAGACAC CGATTTCGAC TCCCGAAAAA 
    ACGACGTCCA CTCCCTCGGA TATCCAGCTT GAAGTTGCAT TTGTAAGCCC ATTGGTGCTC 
    CGTCGTGAGC TTGAAACACT CCTATCCACT GATAAGGACG CGATGAGGAA TGAATCGCTG 
    AAAACGACGC ATCCGGTGGT TTTCTGGAAT TTGATATACT ATTTGAGAAG ACTCTCCCTT 
    CCGAGCCATT TATTCTCGTG GATTGCCGAA CGTCATCACA TTCGTTGCGT CTTTGATCTA 
    CCAACACAAC ACGACGAAAC CCGTCTCCCA TTGTACTTTT ACAACCCGAA TCACGATGGA 
    AACGCGCCAA GTTTCGGCGA TTTTACCGAA AAAATCACCC GAAAATTGGA GGATGGCTCG 
    GATTCGACGG GCTATGCTAC ATGGAAGACG GTCACCGACT CGGTGAACAA GAATTTGCTG 
    TTTAAGGCAG TTCAGACACT GGTCAACTCG TCGCGATTGG TGAAAAATGG AAGTGTTCAT 
    GTTGGAGCAC ATTTTCCCAT TTTTAGAGAC ATTCAATTCG CATCGATTGA CGTATTTGGC 
    AGAGCACTGC TTCGTGATGC TCTCGACACT CAATACGACT CGGAATCCAA CAAATTACCG 
    CCGAAAATCA AGGAAATCCT TCCGCTTCAG GATCATCCGT TGTCCCATGT CACCCGAGCG 
    TGCCGCAAAG TTTTCATGCC ATTGGACCTA TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307460.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294389.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.
    Price & Availability↑
    CloneID OCa344514
    Clone ID Related Accession (Same CDS sequence) NM_001307460.1 , NM_001307460.2
    Accession Version NM_001307460.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGGTCT TCATCCCGGC ACCGGAGCCA TTTTTGAATC AGGAGACTGG CGAAGAGCTG 
    CAATTTGTCT ATGGGGAATT CCCGTCGAAA ATGCGAAGCG AGTACTTCCA ACTGGATCGA 
    CTTGAGAAAA ATGGGAAAGA TGGGGAAAAG GTTCCCATGC ATTTTGAAGC ATCCCGTTGC 
    TCGGCAGTTC GCACAAAACC GGAAACCCGT TCCTCGTTGC TAGCCGCCAC AAATGCTGTG 
    AAAACCAACC CGCTTCATTG GTCAAAAACG CTTCTGTTCT ACTCATATTC ACTATGGTTC 
    ATGCAATTGG ATAGCCTCCT CACCGCTGCT CCAAACAAGA AGAAAATACT TCGTCTGGCA 
    TTTAATGTGC TCGATCGAAT GGAAAAAACC GAGATTTTCC CGCTCGATCA AGTATGCTAT 
    CGAATTCTTA TCGAACTTTG CGGAAAATAT TCGGAGCCCG AGATGGCTGT CAAGGTTATG 
    AGAGCCATGC AAAGAGCTGG GCTTGAACAG AACGCCGTCA CCTATGGAAT CTACCACCGC 
    GCCGTTATGG ATGCCACGTG GCCGTCGCCA GCTCGCCAAA AAGCCATAAA AATGTGGAAT 
    TTGCTGAGAA TTGTGCTCAC CGGCGCCGAA ACTCTCCGAA AATGGGCGAA TTTGGAAAGA 
    AGACCGCTGA AAATTGATGA AAAATCGGTG GAAAACACGG AAAATGCTGA GAATGTGCAG 
    GCGGAGATCA CGGTGGATTA TCAGATTACC AGCTATCCAC TGGATTCTGT GGAAAATTCG 
    CCGAAAATGA CCCCAAAACG GCACAATTTG GAGCAAAAAG AGCCGCTGAT GGATCCACTG 
    GGTGCTCTGG GCCCCCTGGA AGCTGAAAAT TTGGAAGTTT CACGCGAAAA AACCAAGGAA 
    CGATCAAAAT CGCTGGGAAA AGTTGGAATG TCGCCAGCGA GAGCGAAATT CTTTGCCGAT 
    CACGCTACAC TGCCATTTTC CAACGAAAAT ACGCCGAAAT CCGATAAAAA AGAGGCAAAA 
    AGTGGATGGT TTAAGGGAAT TGCGAATAGT CCAATGTTTA AAATGATTAA AAGTACTACT 
    TTTGATTCTT CCAAACAAAA CTCTGCTGAA TCATCAAGTG AAACTAGCTC AATAACCGGA 
    GCTTCTCCAT CATTCCATTC CGTAGTAAAT CAATTCAAAA AGGGATACGA TGACGTCATG 
    CCGTCTCGAT TCCGTCTCGG CGTTTCCACA CTATTGAACA AATCCGTCGG TGCAATGGGC 
    TCTGGAATGA TTTATTCGGA CGATCGGGCT TCTGCGGAAA ATCCGTTCTT TGTGAATCCA 
    AAAGATCCGG CTTATGTGCT AGATTCGGGC GTACCGAACT GTCTGCTGCG GGAGGAATAT 
    TGGATGCGTG AGGTGTATTT GCAGTTGAAA CGGAGCCGTG AGCGGGTTGA AAAGGGTCAG 
    AAAAGCGAGG AAAACGAGGA GATTTCCGAT GGAATAGCGG ATTTCCCCCA ATTCTCCGCA 
    AAAACTCCAG GAAAACTGGA GGTGATCCTG TGCACCGCCA CCTACTGCCC GTCATGCCGA 
    ACAATGGTCT ACGATGAGGA AATCATGGCC GGCTGGAAAG TTGACGATCA GAATTTGAAC 
    ACAATATGCC CACACTGCTC GGGAAGCGGA ACGGCGACAG ACGCCGTCTC CAACGAAACC 
    GCAGCCATTC AGGAGAGCGT TTTCGCGCCG AGACTCAAAA TTCGAATGAG ATGGATTGAG 
    GAGATGAAGA GTAGTTGGTA TCAGCCTGGT GGACTGCAGG AGACACCGAT TTCGACTCCC 
    GAAAAAACGA CGTCCACTCC CTCGGATATC CAGCTTGAAG TTGCATTTGT AAGCCCATTG 
    GTGCTCCGTC GTGAGCTTGA AACACTCCTA TCCACTGATA AGGACGCGAT GAGGAATGAA 
    TCGCTGAAAA CGACGCATCC GGTGGTTTTC TGGAATTTGA TATACTATTT GAGAAGACTC 
    TCCCTTCCGA GCCATTTATT CTCGTGGATT GCCGAACGTC ATCACATTCG TTGCGTCTTT 
    GATCTACCAA CACAACACGA CGAAACCCGT CTCCCATTGT ACTTTTACAA CCCGAATCAC 
    GATGGAAACG CGCCAAGTTT CGGCGATTTT ACCGAAAAAA TCACCCGAAA ATTGGAGGAT 
    GGCTCGGATT CGACGGGCTA TGCTACATGG AAGACGGTCA CCGACTCGGT GAACAAGAAT 
    TTGCTGTTTA AGGCAGTTCA GACACTGGTC AACTCGTCGC GATTGGTGAA AAATGGAAGT 
    GTTCATGTTG GAGCACATTT TCCCATTTTT AGAGACATTC AATTCGCATC GATTGACGTA 
    TTTGGCAGAG CACTGCTTCG TGATGCTCTC GACACTCAAT ACGACTCGGA ATCCAACAAA 
    TTACCGCCGA AAATCAAGGA AATCCTTCCG CTTCAGGATC ATCCGTTGTC CCATGTCACC 
    CGAGCGTGCC GCAAAGTTTT CATGCCATTG GACCTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294389.1
    CDS1..4602
    Translation

    Target ORF information:

    RefSeq Version NM_001307460.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307460.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGGTCT TCATCCCGGC ACCGGAGCCA TTTTTGAATC AGGAGACTGG CGAAGAGCTG 
    CAATTTGTCT ATGGGGAATT CCCGTCGAAA ATGCGAAGCG AGTACTTCCA ACTGGATCGA 
    CTTGAGAAAA ATGGGAAAGA TGGGGAAAAG GTTCCCATGC ATTTTGAAGC ATCCCGTTGC 
    TCGGCAGTTC GCACAAAACC GGAAACCCGT TCCTCGTTGC TAGCCGCCAC AAATGCTGTG 
    AAAACCAACC CGCTTCATTG GTCAAAAACG CTTCTGTTCT ACTCATATTC ACTATGGTTC 
    ATGCAATTGG ATAGCCTCCT CACCGCTGCT CCAAACAAGA AGAAAATACT TCGTCTGGCA 
    TTTAATGTGC TCGATCGAAT GGAAAAAACC GAGATTTTCC CGCTCGATCA AGTATGCTAT 
    CGAATTCTTA TCGAACTTTG CGGAAAATAT TCGGAGCCCG AGATGGCTGT CAAGGTTATG 
    AGAGCCATGC AAAGAGCTGG GCTTGAACAG AACGCCGTCA CCTATGGAAT CTACCACCGC 
    GCCGTTATGG ATGCCACGTG GCCGTCGCCA GCTCGCCAAA AAGCCATAAA AATGTGGAAT 
    TTGCTGAGAA TTGTGCTCAC CGGCGCCGAA ACTCTCCGAA AATGGGCGAA TTTGGAAAGA 
    AGACCGCTGA AAATTGATGA AAAATCGGTG GAAAACACGG AAAATGCTGA GAATGTGCAG 
    GCGGAGATCA CGGTGGATTA TCAGATTACC AGCTATCCAC TGGATTCTGT GGAAAATTCG 
    CCGAAAATGA CCCCAAAACG GCACAATTTG GAGCAAAAAG AGCCGCTGAT GGATCCACTG 
    GGTGCTCTGG GCCCCCTGGA AGCTGAAAAT TTGGAAGTTT CACGCGAAAA AACCAAGGAA 
    CGATCAAAAT CGCTGGGAAA AGTTGGAATG TCGCCAGCGA GAGCGAAATT CTTTGCCGAT 
    CACGCTACAC TGCCATTTTC CAACGAAAAT ACGCCGAAAT CCGATAAAAA AGAGGCAAAA 
    AGTGGATGGT TTAAGGGAAT TGCGAATAGT CCAATGTTTA AAATGATTAA AAGTACTACT 
    TTTGATTCTT CCAAACAAAA CTCTGCTGAA TCATCAAGTG AAACTAGCTC AATAACCGGA 
    GCTTCTCCAT CATTCCATTC CGTAGTAAAT CAATTCAAAA AGGGATACGA TGACGTCATG 
    CCGTCTCGAT TCCGTCTCGG CGTTTCCACA CTATTGAACA AATCCGTCGG TGCAATGGGC 
    TCTGGAATGA TTTATTCGGA CGATCGGGCT TCTGCGGAAA ATCCGTTCTT TGTGAATCCA 
    AAAGATCCGG CTTATGTGCT AGATTCGGGC GTACCGAACT GTCTGCTGCG GGAGGAATAT 
    TGGATGCGTG AGGTGTATTT GCAGTTGAAA CGGAGCCGTG AGCGGGTTGA AAAGGGTCAG 
    AAAAGCGAGG AAAACGAGGA GATTTCCGAT GGAATAGCGG ATTTCCCCCA ATTCTCCGCA 
    AAAACTCCAG GAAAACTGGA GGTGATCCTG TGCACCGCCA CCTACTGCCC GTCATGCCGA 
    ACAATGGTCT ACGATGAGGA AATCATGGCC GGCTGGAAAG TTGACGATCA GAATTTGAAC 
    ACAATATGCC CACACTGCTC GGGAAGCGGA ACGGCGACAG ACGCCGTCTC CAACGAAACC 
    GCAGCCATTC AGGAGAGCGT TTTCGCGCCG AGACTCAAAA TTCGAATGAG ATGGATTGAG 
    GAGATGAAGA GTAGTTGGTA TCAGCCTGGT GGACTGCAGG AGACACCGAT TTCGACTCCC 
    GAAAAAACGA CGTCCACTCC CTCGGATATC CAGCTTGAAG TTGCATTTGT AAGCCCATTG 
    GTGCTCCGTC GTGAGCTTGA AACACTCCTA TCCACTGATA AGGACGCGAT GAGGAATGAA 
    TCGCTGAAAA CGACGCATCC GGTGGTTTTC TGGAATTTGA TATACTATTT GAGAAGACTC 
    TCCCTTCCGA GCCATTTATT CTCGTGGATT GCCGAACGTC ATCACATTCG TTGCGTCTTT 
    GATCTACCAA CACAACACGA CGAAACCCGT CTCCCATTGT ACTTTTACAA CCCGAATCAC 
    GATGGAAACG CGCCAAGTTT CGGCGATTTT ACCGAAAAAA TCACCCGAAA ATTGGAGGAT 
    GGCTCGGATT CGACGGGCTA TGCTACATGG AAGACGGTCA CCGACTCGGT GAACAAGAAT 
    TTGCTGTTTA AGGCAGTTCA GACACTGGTC AACTCGTCGC GATTGGTGAA AAATGGAAGT 
    GTTCATGTTG GAGCACATTT TCCCATTTTT AGAGACATTC AATTCGCATC GATTGACGTA 
    TTTGGCAGAG CACTGCTTCG TGATGCTCTC GACACTCAAT ACGACTCGGA ATCCAACAAA 
    TTACCGCCGA AAATCAAGGA AATCCTTCCG CTTCAGGATC ATCCGTTGTC CCATGTCACC 
    CGAGCGTGCC GCAAAGTTTT CATGCCATTG GACCTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307460.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294389.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA.
    Price & Availability↑
    CloneID OCa344514
    Clone ID Related Accession (Same CDS sequence) NM_001307460.1 , NM_001307460.2
    Accession Version NM_001307460.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307460.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGGTCT TCATCCCGGC ACCGGAGCCA TTTTTGAATC AGGAGACTGG CGAAGAGCTG 
    CAATTTGTCT ATGGGGAATT CCCGTCGAAA ATGCGAAGCG AGTACTTCCA ACTGGATCGA 
    CTTGAGAAAA ATGGGAAAGA TGGGGAAAAG GTTCCCATGC ATTTTGAAGC ATCCCGTTGC 
    TCGGCAGTTC GCACAAAACC GGAAACCCGT TCCTCGTTGC TAGCCGCCAC AAATGCTGTG 
    AAAACCAACC CGCTTCATTG GTCAAAAACG CTTCTGTTCT ACTCATATTC ACTATGGTTC 
    ATGCAATTGG ATAGCCTCCT CACCGCTGCT CCAAACAAGA AGAAAATACT TCGTCTGGCA 
    TTTAATGTGC TCGATCGAAT GGAAAAAACC GAGATTTTCC CGCTCGATCA AGTATGCTAT 
    CGAATTCTTA TCGAACTTTG CGGAAAATAT TCGGAGCCCG AGATGGCTGT CAAGGTTATG 
    AGAGCCATGC AAAGAGCTGG GCTTGAACAG AACGCCGTCA CCTATGGAAT CTACCACCGC 
    GCCGTTATGG ATGCCACGTG GCCGTCGCCA GCTCGCCAAA AAGCCATAAA AATGTGGAAT 
    TTGCTGAGAA TTGTGCTCAC CGGCGCCGAA ACTCTCCGAA AATGGGCGAA TTTGGAAAGA 
    AGACCGCTGA AAATTGATGA AAAATCGGTG GAAAACACGG AAAATGCTGA GAATGTGCAG 
    GCGGAGATCA CGGTGGATTA TCAGATTACC AGCTATCCAC TGGATTCTGT GGAAAATTCG 
    CCGAAAATGA CCCCAAAACG GCACAATTTG GAGCAAAAAG AGCCGCTGAT GGATCCACTG 
    GGTGCTCTGG GCCCCCTGGA AGCTGAAAAT TTGGAAGTTT CACGCGAAAA AACCAAGGAA 
    CGATCAAAAT CGCTGGGAAA AGTTGGAATG TCGCCAGCGA GAGCGAAATT CTTTGCCGAT 
    CACGCTACAC TGCCATTTTC CAACGAAAAT ACGCCGAAAT CCGATAAAAA AGAGGCAAAA 
    AGTGGATGGT TTAAGGGAAT TGCGAATAGT CCAATGTTTA AAATGATTAA AAGTACTACT 
    TTTGATTCTT CCAAACAAAA CTCTGCTGAA TCATCAAGTG AAACTAGCTC AATAACCGGA 
    GCTTCTCCAT CATTCCATTC CGTAGTAAAT CAATTCAAAA AGGGATACGA TGACGTCATG 
    CCGTCTCGAT TCCGTCTCGG CGTTTCCACA CTATTGAACA AATCCGTCGG TGCAATGGGC 
    TCTGGAATGA TTTATTCGGA CGATCGGGCT TCTGCGGAAA ATCCGTTCTT TGTGAATCCA 
    AAAGATCCGG CTTATGTGCT AGATTCGGGC GTACCGAACT GTCTGCTGCG GGAGGAATAT 
    TGGATGCGTG AGGTGTATTT GCAGTTGAAA CGGAGCCGTG AGCGGGTTGA AAAGGGTCAG 
    AAAAGCGAGG AAAACGAGGA GATTTCCGAT GGAATAGCGG ATTTCCCCCA ATTCTCCGCA 
    AAAACTCCAG GAAAACTGGA GGTGATCCTG TGCACCGCCA CCTACTGCCC GTCATGCCGA 
    ACAATGGTCT ACGATGAGGA AATCATGGCC GGCTGGAAAG TTGACGATCA GAATTTGAAC 
    ACAATATGCC CACACTGCTC GGGAAGCGGA ACGGCGACAG ACGCCGTCTC CAACGAAACC 
    GCAGCCATTC AGGAGAGCGT TTTCGCGCCG AGACTCAAAA TTCGAATGAG ATGGATTGAG 
    GAGATGAAGA GTAGTTGGTA TCAGCCTGGT GGACTGCAGG AGACACCGAT TTCGACTCCC 
    GAAAAAACGA CGTCCACTCC CTCGGATATC CAGCTTGAAG TTGCATTTGT AAGCCCATTG 
    GTGCTCCGTC GTGAGCTTGA AACACTCCTA TCCACTGATA AGGACGCGAT GAGGAATGAA 
    TCGCTGAAAA CGACGCATCC GGTGGTTTTC TGGAATTTGA TATACTATTT GAGAAGACTC 
    TCCCTTCCGA GCCATTTATT CTCGTGGATT GCCGAACGTC ATCACATTCG TTGCGTCTTT 
    GATCTACCAA CACAACACGA CGAAACCCGT CTCCCATTGT ACTTTTACAA CCCGAATCAC 
    GATGGAAACG CGCCAAGTTT CGGCGATTTT ACCGAAAAAA TCACCCGAAA ATTGGAGGAT 
    GGCTCGGATT CGACGGGCTA TGCTACATGG AAGACGGTCA CCGACTCGGT GAACAAGAAT 
    TTGCTGTTTA AGGCAGTTCA GACACTGGTC AACTCGTCGC GATTGGTGAA AAATGGAAGT 
    GTTCATGTTG GAGCACATTT TCCCATTTTT AGAGACATTC AATTCGCATC GATTGACGTA 
    TTTGGCAGAG CACTGCTTCG TGATGCTCTC GACACTCAAT ACGACTCGGA ATCCAACAAA 
    TTACCGCCGA AAATCAAGGA AATCCTTCCG CTTCAGGATC ATCCGTTGTC CCATGTCACC 
    CGAGCGTGCC GCAAAGTTTT CATGCCATTG GACCTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294389.1
    CDS8..4609
    Translation

    Target ORF information:

    RefSeq Version NM_001307460.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307460.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGGTCT TCATCCCGGC ACCGGAGCCA TTTTTGAATC AGGAGACTGG CGAAGAGCTG 
    CAATTTGTCT ATGGGGAATT CCCGTCGAAA ATGCGAAGCG AGTACTTCCA ACTGGATCGA 
    CTTGAGAAAA ATGGGAAAGA TGGGGAAAAG GTTCCCATGC ATTTTGAAGC ATCCCGTTGC 
    TCGGCAGTTC GCACAAAACC GGAAACCCGT TCCTCGTTGC TAGCCGCCAC AAATGCTGTG 
    AAAACCAACC CGCTTCATTG GTCAAAAACG CTTCTGTTCT ACTCATATTC ACTATGGTTC 
    ATGCAATTGG ATAGCCTCCT CACCGCTGCT CCAAACAAGA AGAAAATACT TCGTCTGGCA 
    TTTAATGTGC TCGATCGAAT GGAAAAAACC GAGATTTTCC CGCTCGATCA AGTATGCTAT 
    CGAATTCTTA TCGAACTTTG CGGAAAATAT TCGGAGCCCG AGATGGCTGT CAAGGTTATG 
    AGAGCCATGC AAAGAGCTGG GCTTGAACAG AACGCCGTCA CCTATGGAAT CTACCACCGC 
    GCCGTTATGG ATGCCACGTG GCCGTCGCCA GCTCGCCAAA AAGCCATAAA AATGTGGAAT 
    TTGCTGAGAA TTGTGCTCAC CGGCGCCGAA ACTCTCCGAA AATGGGCGAA TTTGGAAAGA 
    AGACCGCTGA AAATTGATGA AAAATCGGTG GAAAACACGG AAAATGCTGA GAATGTGCAG 
    GCGGAGATCA CGGTGGATTA TCAGATTACC AGCTATCCAC TGGATTCTGT GGAAAATTCG 
    CCGAAAATGA CCCCAAAACG GCACAATTTG GAGCAAAAAG AGCCGCTGAT GGATCCACTG 
    GGTGCTCTGG GCCCCCTGGA AGCTGAAAAT TTGGAAGTTT CACGCGAAAA AACCAAGGAA 
    CGATCAAAAT CGCTGGGAAA AGTTGGAATG TCGCCAGCGA GAGCGAAATT CTTTGCCGAT 
    CACGCTACAC TGCCATTTTC CAACGAAAAT ACGCCGAAAT CCGATAAAAA AGAGGCAAAA 
    AGTGGATGGT TTAAGGGAAT TGCGAATAGT CCAATGTTTA AAATGATTAA AAGTACTACT 
    TTTGATTCTT CCAAACAAAA CTCTGCTGAA TCATCAAGTG AAACTAGCTC AATAACCGGA 
    GCTTCTCCAT CATTCCATTC CGTAGTAAAT CAATTCAAAA AGGGATACGA TGACGTCATG 
    CCGTCTCGAT TCCGTCTCGG CGTTTCCACA CTATTGAACA AATCCGTCGG TGCAATGGGC 
    TCTGGAATGA TTTATTCGGA CGATCGGGCT TCTGCGGAAA ATCCGTTCTT TGTGAATCCA 
    AAAGATCCGG CTTATGTGCT AGATTCGGGC GTACCGAACT GTCTGCTGCG GGAGGAATAT 
    TGGATGCGTG AGGTGTATTT GCAGTTGAAA CGGAGCCGTG AGCGGGTTGA AAAGGGTCAG 
    AAAAGCGAGG AAAACGAGGA GATTTCCGAT GGAATAGCGG ATTTCCCCCA ATTCTCCGCA 
    AAAACTCCAG GAAAACTGGA GGTGATCCTG TGCACCGCCA CCTACTGCCC GTCATGCCGA 
    ACAATGGTCT ACGATGAGGA AATCATGGCC GGCTGGAAAG TTGACGATCA GAATTTGAAC 
    ACAATATGCC CACACTGCTC GGGAAGCGGA ACGGCGACAG ACGCCGTCTC CAACGAAACC 
    GCAGCCATTC AGGAGAGCGT TTTCGCGCCG AGACTCAAAA TTCGAATGAG ATGGATTGAG 
    GAGATGAAGA GTAGTTGGTA TCAGCCTGGT GGACTGCAGG AGACACCGAT TTCGACTCCC 
    GAAAAAACGA CGTCCACTCC CTCGGATATC CAGCTTGAAG TTGCATTTGT AAGCCCATTG 
    GTGCTCCGTC GTGAGCTTGA AACACTCCTA TCCACTGATA AGGACGCGAT GAGGAATGAA 
    TCGCTGAAAA CGACGCATCC GGTGGTTTTC TGGAATTTGA TATACTATTT GAGAAGACTC 
    TCCCTTCCGA GCCATTTATT CTCGTGGATT GCCGAACGTC ATCACATTCG TTGCGTCTTT 
    GATCTACCAA CACAACACGA CGAAACCCGT CTCCCATTGT ACTTTTACAA CCCGAATCAC 
    GATGGAAACG CGCCAAGTTT CGGCGATTTT ACCGAAAAAA TCACCCGAAA ATTGGAGGAT 
    GGCTCGGATT CGACGGGCTA TGCTACATGG AAGACGGTCA CCGACTCGGT GAACAAGAAT 
    TTGCTGTTTA AGGCAGTTCA GACACTGGTC AACTCGTCGC GATTGGTGAA AAATGGAAGT 
    GTTCATGTTG GAGCACATTT TCCCATTTTT AGAGACATTC AATTCGCATC GATTGACGTA 
    TTTGGCAGAG CACTGCTTCG TGATGCTCTC GACACTCAAT ACGACTCGGA ATCCAACAAA 
    TTACCGCCGA AAATCAAGGA AATCCTTCCG CTTCAGGATC ATCCGTTGTC CCATGTCACC 
    CGAGCGTGCC GCAAAGTTTT CATGCCATTG GACCTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307461.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294390.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA.
    Price & Availability↑
    CloneID OCa344515
    Clone ID Related Accession (Same CDS sequence) NM_001307461.2 , NM_001307461.1
    Accession Version NM_001307461.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307461.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    AGTACGATTA ACGCGTTAGC TGATACAAGG ACGTCTATGG TCTTCATCCC GGCACCGGAG 
    CCATTTTTGA ATCAGGAGAC TGGCGAAGAG CTGCAATTTG TCTATGGGGA ATTCCCGTCG 
    AAAATGCGAA GCGAGTACTT CCAACTGGAT CGACTTGAGA AAAATGGGAA AGATGGGGAA 
    AAGGTTCCCA TGCATTTTGA AGCATCCCGT TGCTCGGCAG TTCGCACAAA ACCGGAAACC 
    CGTTCCTCGT TGCTAGCCGC CACAAATGCT GTGAAAACCA ACCCGCTTCA TTGGTCAAAA 
    ACGCTTCTGT TCTACTCATA TTCACTATGG TTCATGCAAT TGGATAGCCT CCTCACCGCT 
    GCTCCAAACA AGAAGAAAAT ACTTCGTCTG GCATTTAATG TGCTCGATCG AATGGAAAAA 
    ACCGAGATTT TCCCGCTCGA TCAAGTATGC TATCGAATTC TTATCGAACT TTGCGGAAAA 
    TATTCGGAGC CCGAGATGGC TGTCAAGGTT ATGAGAGCCA TGCAAAGAGC TGGGCTTGAA 
    CAGAACGCCG TCACCTATGG AATCTACCAC CGCGCCGTTA TGGATGCCAC GTGGCCGTCG 
    CCAGCTCGCC AAAAAGCCAT AAAAATGTGG AATTTGCTGA GAATTGTGCT CACCGGCGCC 
    GAAACTCTCC GAAAATGGGC GAATTTGGAA AGAAGACCGC TGAAAATTGA TGAAAAATCG 
    GTGGAAAACA CGGAAAATGC TGAGAATGTG CAGGCGGAGA TCACGGTGGA TTATCAGATT 
    ACCAGCTATC CACTGGATTC TGTGGAAAAT TCGCCGAAAA TGACCCCAAA ACGGCACAAT 
    TTGGAGCAAA AAGAGCCGCT GATGGATCCA CTGGGTGCTC TGGGCCCCCT GGAAGCTGAA 
    AATTTGGAAG TTTCACGCGA AAAAACCAAG GAACGATCAA AATCGCTGGG AAAAGTTGGA 
    ATGTCGCCAG CGAGAGCGAA ATTCTTTGCC GATCACGCTA CACTGCCATT TTCCAACGAA 
    AATACGCCGA AATCCGATAA AAAAGAGGCA AAAAGTGGAT GGTTTAAGGG AATTGCGAAT 
    AGTCCAATGT TTAAAATGAT TAAAAGTACT ACTTTTGATT CTTCCAAACA AAACTCTGCT 
    GAATCATCAA GTGAAACTAG CTCAATAACC GGAGCTTCTC CATCATTCCA TTCCGTAGTA 
    AATCAATTCA AAAAGGGATA CGATGACGTC ATGCCGTCTC GATTCCGTCT CGGCGTTTCC 
    ACACTATTGA ACAAATCCGT CGGTGCAATG GGCTCTGGAA TGATTTATTC GGACGATCGG 
    GCTTCTGCGG AAAATCCGTT CTTTGTGAAT CCAAAAGATC CGGCTTATGT GCTAGATTCG 
    GGCGTACCGA ACTGTCTGCT GCGGGAGGAA TATTGGATGC GTGAGGTGTA TTTGCAGTTG 
    AAACGGAGCC GTGAGCGGGT TGAAAAGGGT CAGAAAAGCG AGGAAAACGA GGAGATTTCC 
    GATGGAATAG CGGATTTCCC CCAATTCTCC GCAAAAACTC CAGGAAAACT GGAGGTGATC 
    CTGTGCACCG CCACCTACTG CCCGTCATGC CGAACAATGG TCTACGATGA GGAAATCATG 
    GCCGGCTGGA AAGTTGACGA TCAGAATTTG AACACAATAT GCCCACACTG CTCGGGAAGC 
    GGAACGGCGA CAGACGCCGT CTCCAACGAA ACCGCAGCCA TTCAGGAGAG CGTTTTCGCG 
    CCGAGACTCA AAATTCGAAT GAGATGGATT GAGGAGATGA AGAGTAGTTG GTATCAGCCT 
    GGTGGACTGC AGGAGACACC GATTTCGACT CCCGAAAAAA CGACGTCCAC TCCCTCGGAT 
    ATCCAGCTTG AAGTTGCATT TGTAAGCCCA TTGGTGCTCC GTCGTGAGCT TGAAACACTC 
    CTATCCACTG ATAAGGACGC GATGAGGAAT GAATCGCTGA AAACGACGCA TCCGGTGGTT 
    TTCTGGAATT TGATATACTA TTTGAGAAGA CTCTCCCTTC CGAGCCATTT ATTCTCGTGG 
    ATTGCCGAAC GTCATCACAT TCGTTGCGTC TTTGATCTAC CAACACAACA CGACGAAACC 
    CGTCTCCCAT TGTACTTTTA CAACCCGAAT CACGATGGAA ACGCGCCAAG TTTCGGCGAT 
    TTTACCGAAA AAATCACCCG AAAATTGGAG GATGGCTCGG ATTCGACGGG CTATGCTACA 
    TGGAAGACGG TCACCGACTC GGTGAACAAG AATTTGCTGT TTAAGGCAGT TCAGACACTG 
    GTCAACTCGT CGCGATTGGT GAAAAATGGA AGTGTTCATG TTGGAGCACA TTTTCCCATT 
    TTTAGAGACA TTCAATTCGC ATCGATTGAC GTATTTGGCA GAGCACTGCT TCGTGATGCT 
    CTCGACACTC AATACGACTC GGAATCCAAC AAATTACCGC CGAAAATCAA GGAAATCCTT 
    CCGCTTCAGG ATCATCCGTT GTCCCATGTC ACCCGAGCGT GCCGCAAAGT TTTCATGCCA 
    TTGGACCTAT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294390.1
    CDS7..4641
    Translation

    Target ORF information:

    RefSeq Version NM_001307461.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307461.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    AGTACGATTA ACGCGTTAGC TGATACAAGG ACGTCTATGG TCTTCATCCC GGCACCGGAG 
    CCATTTTTGA ATCAGGAGAC TGGCGAAGAG CTGCAATTTG TCTATGGGGA ATTCCCGTCG 
    AAAATGCGAA GCGAGTACTT CCAACTGGAT CGACTTGAGA AAAATGGGAA AGATGGGGAA 
    AAGGTTCCCA TGCATTTTGA AGCATCCCGT TGCTCGGCAG TTCGCACAAA ACCGGAAACC 
    CGTTCCTCGT TGCTAGCCGC CACAAATGCT GTGAAAACCA ACCCGCTTCA TTGGTCAAAA 
    ACGCTTCTGT TCTACTCATA TTCACTATGG TTCATGCAAT TGGATAGCCT CCTCACCGCT 
    GCTCCAAACA AGAAGAAAAT ACTTCGTCTG GCATTTAATG TGCTCGATCG AATGGAAAAA 
    ACCGAGATTT TCCCGCTCGA TCAAGTATGC TATCGAATTC TTATCGAACT TTGCGGAAAA 
    TATTCGGAGC CCGAGATGGC TGTCAAGGTT ATGAGAGCCA TGCAAAGAGC TGGGCTTGAA 
    CAGAACGCCG TCACCTATGG AATCTACCAC CGCGCCGTTA TGGATGCCAC GTGGCCGTCG 
    CCAGCTCGCC AAAAAGCCAT AAAAATGTGG AATTTGCTGA GAATTGTGCT CACCGGCGCC 
    GAAACTCTCC GAAAATGGGC GAATTTGGAA AGAAGACCGC TGAAAATTGA TGAAAAATCG 
    GTGGAAAACA CGGAAAATGC TGAGAATGTG CAGGCGGAGA TCACGGTGGA TTATCAGATT 
    ACCAGCTATC CACTGGATTC TGTGGAAAAT TCGCCGAAAA TGACCCCAAA ACGGCACAAT 
    TTGGAGCAAA AAGAGCCGCT GATGGATCCA CTGGGTGCTC TGGGCCCCCT GGAAGCTGAA 
    AATTTGGAAG TTTCACGCGA AAAAACCAAG GAACGATCAA AATCGCTGGG AAAAGTTGGA 
    ATGTCGCCAG CGAGAGCGAA ATTCTTTGCC GATCACGCTA CACTGCCATT TTCCAACGAA 
    AATACGCCGA AATCCGATAA AAAAGAGGCA AAAAGTGGAT GGTTTAAGGG AATTGCGAAT 
    AGTCCAATGT TTAAAATGAT TAAAAGTACT ACTTTTGATT CTTCCAAACA AAACTCTGCT 
    GAATCATCAA GTGAAACTAG CTCAATAACC GGAGCTTCTC CATCATTCCA TTCCGTAGTA 
    AATCAATTCA AAAAGGGATA CGATGACGTC ATGCCGTCTC GATTCCGTCT CGGCGTTTCC 
    ACACTATTGA ACAAATCCGT CGGTGCAATG GGCTCTGGAA TGATTTATTC GGACGATCGG 
    GCTTCTGCGG AAAATCCGTT CTTTGTGAAT CCAAAAGATC CGGCTTATGT GCTAGATTCG 
    GGCGTACCGA ACTGTCTGCT GCGGGAGGAA TATTGGATGC GTGAGGTGTA TTTGCAGTTG 
    AAACGGAGCC GTGAGCGGGT TGAAAAGGGT CAGAAAAGCG AGGAAAACGA GGAGATTTCC 
    GATGGAATAG CGGATTTCCC CCAATTCTCC GCAAAAACTC CAGGAAAACT GGAGGTGATC 
    CTGTGCACCG CCACCTACTG CCCGTCATGC CGAACAATGG TCTACGATGA GGAAATCATG 
    GCCGGCTGGA AAGTTGACGA TCAGAATTTG AACACAATAT GCCCACACTG CTCGGGAAGC 
    GGAACGGCGA CAGACGCCGT CTCCAACGAA ACCGCAGCCA TTCAGGAGAG CGTTTTCGCG 
    CCGAGACTCA AAATTCGAAT GAGATGGATT GAGGAGATGA AGAGTAGTTG GTATCAGCCT 
    GGTGGACTGC AGGAGACACC GATTTCGACT CCCGAAAAAA CGACGTCCAC TCCCTCGGAT 
    ATCCAGCTTG AAGTTGCATT TGTAAGCCCA TTGGTGCTCC GTCGTGAGCT TGAAACACTC 
    CTATCCACTG ATAAGGACGC GATGAGGAAT GAATCGCTGA AAACGACGCA TCCGGTGGTT 
    TTCTGGAATT TGATATACTA TTTGAGAAGA CTCTCCCTTC CGAGCCATTT ATTCTCGTGG 
    ATTGCCGAAC GTCATCACAT TCGTTGCGTC TTTGATCTAC CAACACAACA CGACGAAACC 
    CGTCTCCCAT TGTACTTTTA CAACCCGAAT CACGATGGAA ACGCGCCAAG TTTCGGCGAT 
    TTTACCGAAA AAATCACCCG AAAATTGGAG GATGGCTCGG ATTCGACGGG CTATGCTACA 
    TGGAAGACGG TCACCGACTC GGTGAACAAG AATTTGCTGT TTAAGGCAGT TCAGACACTG 
    GTCAACTCGT CGCGATTGGT GAAAAATGGA AGTGTTCATG TTGGAGCACA TTTTCCCATT 
    TTTAGAGACA TTCAATTCGC ATCGATTGAC GTATTTGGCA GAGCACTGCT TCGTGATGCT 
    CTCGACACTC AATACGACTC GGAATCCAAC AAATTACCGC CGAAAATCAA GGAAATCCTT 
    CCGCTTCAGG ATCATCCGTT GTCCCATGTC ACCCGAGCGT GCCGCAAAGT TTTCATGCCA 
    TTGGACCTAT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307461.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294390.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.
    Price & Availability↑
    CloneID OCa344515
    Clone ID Related Accession (Same CDS sequence) NM_001307461.2 , NM_001307461.1
    Accession Version NM_001307461.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    AGTACGATTA ACGCGTTAGC TGATACAAGG ACGTCTATGG TCTTCATCCC GGCACCGGAG 
    CCATTTTTGA ATCAGGAGAC TGGCGAAGAG CTGCAATTTG TCTATGGGGA ATTCCCGTCG 
    AAAATGCGAA GCGAGTACTT CCAACTGGAT CGACTTGAGA AAAATGGGAA AGATGGGGAA 
    AAGGTTCCCA TGCATTTTGA AGCATCCCGT TGCTCGGCAG TTCGCACAAA ACCGGAAACC 
    CGTTCCTCGT TGCTAGCCGC CACAAATGCT GTGAAAACCA ACCCGCTTCA TTGGTCAAAA 
    ACGCTTCTGT TCTACTCATA TTCACTATGG TTCATGCAAT TGGATAGCCT CCTCACCGCT 
    GCTCCAAACA AGAAGAAAAT ACTTCGTCTG GCATTTAATG TGCTCGATCG AATGGAAAAA 
    ACCGAGATTT TCCCGCTCGA TCAAGTATGC TATCGAATTC TTATCGAACT TTGCGGAAAA 
    TATTCGGAGC CCGAGATGGC TGTCAAGGTT ATGAGAGCCA TGCAAAGAGC TGGGCTTGAA 
    CAGAACGCCG TCACCTATGG AATCTACCAC CGCGCCGTTA TGGATGCCAC GTGGCCGTCG 
    CCAGCTCGCC AAAAAGCCAT AAAAATGTGG AATTTGCTGA GAATTGTGCT CACCGGCGCC 
    GAAACTCTCC GAAAATGGGC GAATTTGGAA AGAAGACCGC TGAAAATTGA TGAAAAATCG 
    GTGGAAAACA CGGAAAATGC TGAGAATGTG CAGGCGGAGA TCACGGTGGA TTATCAGATT 
    ACCAGCTATC CACTGGATTC TGTGGAAAAT TCGCCGAAAA TGACCCCAAA ACGGCACAAT 
    TTGGAGCAAA AAGAGCCGCT GATGGATCCA CTGGGTGCTC TGGGCCCCCT GGAAGCTGAA 
    AATTTGGAAG TTTCACGCGA AAAAACCAAG GAACGATCAA AATCGCTGGG AAAAGTTGGA 
    ATGTCGCCAG CGAGAGCGAA ATTCTTTGCC GATCACGCTA CACTGCCATT TTCCAACGAA 
    AATACGCCGA AATCCGATAA AAAAGAGGCA AAAAGTGGAT GGTTTAAGGG AATTGCGAAT 
    AGTCCAATGT TTAAAATGAT TAAAAGTACT ACTTTTGATT CTTCCAAACA AAACTCTGCT 
    GAATCATCAA GTGAAACTAG CTCAATAACC GGAGCTTCTC CATCATTCCA TTCCGTAGTA 
    AATCAATTCA AAAAGGGATA CGATGACGTC ATGCCGTCTC GATTCCGTCT CGGCGTTTCC 
    ACACTATTGA ACAAATCCGT CGGTGCAATG GGCTCTGGAA TGATTTATTC GGACGATCGG 
    GCTTCTGCGG AAAATCCGTT CTTTGTGAAT CCAAAAGATC CGGCTTATGT GCTAGATTCG 
    GGCGTACCGA ACTGTCTGCT GCGGGAGGAA TATTGGATGC GTGAGGTGTA TTTGCAGTTG 
    AAACGGAGCC GTGAGCGGGT TGAAAAGGGT CAGAAAAGCG AGGAAAACGA GGAGATTTCC 
    GATGGAATAG CGGATTTCCC CCAATTCTCC GCAAAAACTC CAGGAAAACT GGAGGTGATC 
    CTGTGCACCG CCACCTACTG CCCGTCATGC CGAACAATGG TCTACGATGA GGAAATCATG 
    GCCGGCTGGA AAGTTGACGA TCAGAATTTG AACACAATAT GCCCACACTG CTCGGGAAGC 
    GGAACGGCGA CAGACGCCGT CTCCAACGAA ACCGCAGCCA TTCAGGAGAG CGTTTTCGCG 
    CCGAGACTCA AAATTCGAAT GAGATGGATT GAGGAGATGA AGAGTAGTTG GTATCAGCCT 
    GGTGGACTGC AGGAGACACC GATTTCGACT CCCGAAAAAA CGACGTCCAC TCCCTCGGAT 
    ATCCAGCTTG AAGTTGCATT TGTAAGCCCA TTGGTGCTCC GTCGTGAGCT TGAAACACTC 
    CTATCCACTG ATAAGGACGC GATGAGGAAT GAATCGCTGA AAACGACGCA TCCGGTGGTT 
    TTCTGGAATT TGATATACTA TTTGAGAAGA CTCTCCCTTC CGAGCCATTT ATTCTCGTGG 
    ATTGCCGAAC GTCATCACAT TCGTTGCGTC TTTGATCTAC CAACACAACA CGACGAAACC 
    CGTCTCCCAT TGTACTTTTA CAACCCGAAT CACGATGGAA ACGCGCCAAG TTTCGGCGAT 
    TTTACCGAAA AAATCACCCG AAAATTGGAG GATGGCTCGG ATTCGACGGG CTATGCTACA 
    TGGAAGACGG TCACCGACTC GGTGAACAAG AATTTGCTGT TTAAGGCAGT TCAGACACTG 
    GTCAACTCGT CGCGATTGGT GAAAAATGGA AGTGTTCATG TTGGAGCACA TTTTCCCATT 
    TTTAGAGACA TTCAATTCGC ATCGATTGAC GTATTTGGCA GAGCACTGCT TCGTGATGCT 
    CTCGACACTC AATACGACTC GGAATCCAAC AAATTACCGC CGAAAATCAA GGAAATCCTT 
    CCGCTTCAGG ATCATCCGTT GTCCCATGTC ACCCGAGCGT GCCGCAAAGT TTTCATGCCA 
    TTGGACCTAT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294390.1
    CDS1..4635
    Translation

    Target ORF information:

    RefSeq Version NM_001307461.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307461.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATA TTCTCTTCTA CAAAAATGTG TCAATATGTC TGATATCGCG TTTTCCGTTC 
    TTCAACTCAT TCAAACGATT TCTCTTCTTT CTACACAGAA TGTCATCGAC ACCGAGTTGT 
    TCACATCCAG TGCCAATTGA ACGATACATT TCTCATTTGA TGTACGAAGT TTCGTTCCCA 
    ACACCCCGCC GCCCACGCGT TTTCATGCAA CTTGGCGCTG AAAATATCAG TTTTGACTCA 
    CATGATGATA GTCAACTCCC GTTGAACGGA GCTCAACTAA TCGATACTCT AAAATATCTT 
    GGAAGTGAAA ATCTCATGTA TTTGATGCTT CTGGCGCTGC TCGAGCAGAA AATTTTGATT 
    CATAGTTTAC GACCATGGAT GCTTGCCGCC GTAGCCGAAT CAGTGTGTGC TCTGATGTTC 
    CCATTCCATT GGCAATGCCC ATACATCCCA CAATGTCCGT TGGGACTTGC CGGCGTACTC 
    CATGCCCCGC TTCCATTCAT CGCCGGCGTC GATTCCCGAT ACTTTGAGCT TTACGAAGAT 
    CCACCTGATG ACGTCACCTG CTTCGATTTG GACACAAGTA CGATCAGTTT TTCGACAGTC 
    CGCGCCACTT TCAAAACGTC GATGTTGCCG AAAAAACCGG CAAAACAGCT GAAATCGGCG 
    TTGGAGGATA TATTTGGAAA GCTGCAGAAA CAGGATTATT CGACAAATTC AAAAAGTGAT 
    GGAGGATTTA TTCCAGTAGA TCAGGATTTG GTAAAACAGC GACAACGACG AGAGTTCGAG 
    AATGAAATTC ACGATGCTTT TTTGAAATTC ATCGCGAGTA TTATGAAGGG ATATCAAGCG 
    TTTTTAAGGC CTATCAAGGG GGCTCCAATG ACTGAACATG CTACAGATAC TGGGAATTTG 
    TTCGATTTGG ATGGGTTTTT AAGATCCCGT GACCGTTCGT CTGCAGACTT TTACAAACGA 
    TTCTCGGAGA CGCAGTCTTT TATGAGATTT ATAGAGGAAC GAAGCTTTGT ATCGGATAAA 
    AACACATATA ATGCATTCTT CGATGATTGT ATTGCAAAGT TGACGAGCGC AGTGGAAGAC 
    GGTCGAGATA TCAGTGGAGT TCAACTTTTA GAGCCCGACA CATCGCACAG CCACACTACG 
    AGTACGATTA ACGCGTTAGC TGATACAAGG ACGTCTATGG TCTTCATCCC GGCACCGGAG 
    CCATTTTTGA ATCAGGAGAC TGGCGAAGAG CTGCAATTTG TCTATGGGGA ATTCCCGTCG 
    AAAATGCGAA GCGAGTACTT CCAACTGGAT CGACTTGAGA AAAATGGGAA AGATGGGGAA 
    AAGGTTCCCA TGCATTTTGA AGCATCCCGT TGCTCGGCAG TTCGCACAAA ACCGGAAACC 
    CGTTCCTCGT TGCTAGCCGC CACAAATGCT GTGAAAACCA ACCCGCTTCA TTGGTCAAAA 
    ACGCTTCTGT TCTACTCATA TTCACTATGG TTCATGCAAT TGGATAGCCT CCTCACCGCT 
    GCTCCAAACA AGAAGAAAAT ACTTCGTCTG GCATTTAATG TGCTCGATCG AATGGAAAAA 
    ACCGAGATTT TCCCGCTCGA TCAAGTATGC TATCGAATTC TTATCGAACT TTGCGGAAAA 
    TATTCGGAGC CCGAGATGGC TGTCAAGGTT ATGAGAGCCA TGCAAAGAGC TGGGCTTGAA 
    CAGAACGCCG TCACCTATGG AATCTACCAC CGCGCCGTTA TGGATGCCAC GTGGCCGTCG 
    CCAGCTCGCC AAAAAGCCAT AAAAATGTGG AATTTGCTGA GAATTGTGCT CACCGGCGCC 
    GAAACTCTCC GAAAATGGGC GAATTTGGAA AGAAGACCGC TGAAAATTGA TGAAAAATCG 
    GTGGAAAACA CGGAAAATGC TGAGAATGTG CAGGCGGAGA TCACGGTGGA TTATCAGATT 
    ACCAGCTATC CACTGGATTC TGTGGAAAAT TCGCCGAAAA TGACCCCAAA ACGGCACAAT 
    TTGGAGCAAA AAGAGCCGCT GATGGATCCA CTGGGTGCTC TGGGCCCCCT GGAAGCTGAA 
    AATTTGGAAG TTTCACGCGA AAAAACCAAG GAACGATCAA AATCGCTGGG AAAAGTTGGA 
    ATGTCGCCAG CGAGAGCGAA ATTCTTTGCC GATCACGCTA CACTGCCATT TTCCAACGAA 
    AATACGCCGA AATCCGATAA AAAAGAGGCA AAAAGTGGAT GGTTTAAGGG AATTGCGAAT 
    AGTCCAATGT TTAAAATGAT TAAAAGTACT ACTTTTGATT CTTCCAAACA AAACTCTGCT 
    GAATCATCAA GTGAAACTAG CTCAATAACC GGAGCTTCTC CATCATTCCA TTCCGTAGTA 
    AATCAATTCA AAAAGGGATA CGATGACGTC ATGCCGTCTC GATTCCGTCT CGGCGTTTCC 
    ACACTATTGA ACAAATCCGT CGGTGCAATG GGCTCTGGAA TGATTTATTC GGACGATCGG 
    GCTTCTGCGG AAAATCCGTT CTTTGTGAAT CCAAAAGATC CGGCTTATGT GCTAGATTCG 
    GGCGTACCGA ACTGTCTGCT GCGGGAGGAA TATTGGATGC GTGAGGTGTA TTTGCAGTTG 
    AAACGGAGCC GTGAGCGGGT TGAAAAGGGT CAGAAAAGCG AGGAAAACGA GGAGATTTCC 
    GATGGAATAG CGGATTTCCC CCAATTCTCC GCAAAAACTC CAGGAAAACT GGAGGTGATC 
    CTGTGCACCG CCACCTACTG CCCGTCATGC CGAACAATGG TCTACGATGA GGAAATCATG 
    GCCGGCTGGA AAGTTGACGA TCAGAATTTG AACACAATAT GCCCACACTG CTCGGGAAGC 
    GGAACGGCGA CAGACGCCGT CTCCAACGAA ACCGCAGCCA TTCAGGAGAG CGTTTTCGCG 
    CCGAGACTCA AAATTCGAAT GAGATGGATT GAGGAGATGA AGAGTAGTTG GTATCAGCCT 
    GGTGGACTGC AGGAGACACC GATTTCGACT CCCGAAAAAA CGACGTCCAC TCCCTCGGAT 
    ATCCAGCTTG AAGTTGCATT TGTAAGCCCA TTGGTGCTCC GTCGTGAGCT TGAAACACTC 
    CTATCCACTG ATAAGGACGC GATGAGGAAT GAATCGCTGA AAACGACGCA TCCGGTGGTT 
    TTCTGGAATT TGATATACTA TTTGAGAAGA CTCTCCCTTC CGAGCCATTT ATTCTCGTGG 
    ATTGCCGAAC GTCATCACAT TCGTTGCGTC TTTGATCTAC CAACACAACA CGACGAAACC 
    CGTCTCCCAT TGTACTTTTA CAACCCGAAT CACGATGGAA ACGCGCCAAG TTTCGGCGAT 
    TTTACCGAAA AAATCACCCG AAAATTGGAG GATGGCTCGG ATTCGACGGG CTATGCTACA 
    TGGAAGACGG TCACCGACTC GGTGAACAAG AATTTGCTGT TTAAGGCAGT TCAGACACTG 
    GTCAACTCGT CGCGATTGGT GAAAAATGGA AGTGTTCATG TTGGAGCACA TTTTCCCATT 
    TTTAGAGACA TTCAATTCGC ATCGATTGAC GTATTTGGCA GAGCACTGCT TCGTGATGCT 
    CTCGACACTC AATACGACTC GGAATCCAAC AAATTACCGC CGAAAATCAA GGAAATCCTT 
    CCGCTTCAGG ATCATCCGTT GTCCCATGTC ACCCGAGCGT GCCGCAAAGT TTTCATGCCA 
    TTGGACCTAT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307462.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294391.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.
    Price & Availability↑
    CloneID OCa344516
    Clone ID Related Accession (Same CDS sequence) NM_001307462.1 , NM_001307462.2
    Accession Version NM_001307462.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGAGTA CGATTAACGC GTTAGCTGAT ACAAGGACGT CTATGGTCTT CATCCCGGCA 
    CCGGAGCCAT TTTTGAATCA GGAGACTGGC GAAGAGCTGC AATTTGTCTA TGGGGAATTC 
    CCGTCGAAAA TGCGAAGCGA GTACTTCCAA CTGGATCGAC TTGAGAAAAA TGGGAAAGAT 
    GGGGAAAAGG TTCCCATGCA TTTTGAAGCA TCCCGTTGCT CGGCAGTTCG CACAAAACCG 
    GAAACCCGTT CCTCGTTGCT AGCCGCCACA AATGCTGTGA AAACCAACCC GCTTCATTGG 
    TCAAAAACGC TTCTGTTCTA CTCATATTCA CTATGGTTCA TGCAATTGGA TAGCCTCCTC 
    ACCGCTGCTC CAAACAAGAA GAAAATACTT CGTCTGGCAT TTAATGTGCT CGATCGAATG 
    GAAAAAACCG AGATTTTCCC GCTCGATCAA GTATGCTATC GAATTCTTAT CGAACTTTGC 
    GGAAAATATT CGGAGCCCGA GATGGCTGTC AAGGTTATGA GAGCCATGCA AAGAGCTGGG 
    CTTGAACAGA ACGCCGTCAC CTATGGAATC TACCACCGCG CCGTTATGGA TGCCACGTGG 
    CCGTCGCCAG CTCGCCAAAA AGCCATAAAA ATGTGGAATT TGCTGAGAAT TGTGCTCACC 
    GGCGCCGAAA CTCTCCGAAA ATGGGCGAAT TTGGAAAGAA GACCGCTGAA AATTGATGAA 
    AAATCGGTGG AAAACACGGA AAATGCTGAG AATGTGCAGG CGGAGATCAC GGTGGATTAT 
    CAGATTACCA GCTATCCACT GGATTCTGTG GAAAATTCGC CGAAAATGAC CCCAAAACGG 
    CACAATTTGG AGCAAAAAGA GCCGCTGATG GATCCACTGG GTGCTCTGGG CCCCCTGGAA 
    GCTGAAAATT TGGAAGTTTC ACGCGAAAAA ACCAAGGAAC GATCAAAATC GCTGGGAAAA 
    GTTGGAATGT CGCCAGCGAG AGCGAAATTC TTTGCCGATC ACGCTACACT GCCATTTTCC 
    AACGAAAATA CGCCGAAATC CGATAAAAAA GAGGCAAAAA GTGGATGGTT TAAGGGAATT 
    GCGAATAGTC CAATGTTTAA AATGATTAAA AGTACTACTT TTGATTCTTC CAAACAAAAC 
    TCTGCTGAAT CATCAAGTGA AACTAGCTCA ATAACCGGAG CTTCTCCATC ATTCCATTCC 
    GTAGTAAATC AATTCAAAAA GGGATACGAT GACGTCATGC CGTCTCGATT CCGTCTCGGC 
    GTTTCCACAC TATTGAACAA ATCCGTCGGT GCAATGGGCT CTGGAATGAT TTATTCGGAC 
    GATCGGGCTT CTGCGGAAAA TCCGTTCTTT GTGAATCCAA AAGATCCGGC TTATGTGCTA 
    GATTCGGGCG TACCGAACTG TCTGCTGCGG GAGGAATATT GGATGCGTGA GGTGTATTTG 
    CAGTTGAAAC GGAGCCGTGA GCGGGTTGAA AAGGGTCAGA AAAGCGAGGA AAACGAGGAG 
    ATTTCCGATG GAATAGCGGA TTTCCCCCAA TTCTCCGCAA AAACTCCAGG AAAACTGGAG 
    GTGATCCTGT GCACCGCCAC CTACTGCCCG TCATGCCGAA CAATGGTCTA CGATGAGGAA 
    ATCATGGCCG GCTGGAAAGT TGACGATCAG AATTTGAACA CAATATGCCC ACACTGCTCG 
    GGAAGCGGAA CGGCGACAGA CGCCGTCTCC AACGAAACCG CAGCCATTCA GGAGAGCGTT 
    TTCGCGCCGA GACTCAAAAT TCGAATGAGA TGGATTGAGG AGATGAAGAG TAGTTGGTAT 
    CAGCCTGGTG GACTGCAGGA GACACCGATT TCGACTCCCG AAAAAACGAC GTCCACTCCC 
    TCGGATATCC AGCTTGAAGT TGCATTTGTA AGCCCATTGG TGCTCCGTCG TGAGCTTGAA 
    ACACTCCTAT CCACTGATAA GGACGCGATG AGGAATGAAT CGCTGAAAAC GACGCATCCG 
    GTGGTTTTCT GGAATTTGAT ATACTATTTG AGAAGACTCT CCCTTCCGAG CCATTTATTC 
    TCGTGGATTG CCGAACGTCA TCACATTCGT TGCGTCTTTG ATCTACCAAC ACAACACGAC 
    GAAACCCGTC TCCCATTGTA CTTTTACAAC CCGAATCACG ATGGAAACGC GCCAAGTTTC 
    GGCGATTTTA CCGAAAAAAT CACCCGAAAA TTGGAGGATG GCTCGGATTC GACGGGCTAT 
    GCTACATGGA AGACGGTCAC CGACTCGGTG AACAAGAATT TGCTGTTTAA GGCAGTTCAG 
    ACACTGGTCA ACTCGTCGCG ATTGGTGAAA AATGGAAGTG TTCATGTTGG AGCACATTTT 
    CCCATTTTTA GAGACATTCA ATTCGCATCG ATTGACGTAT TTGGCAGAGC ACTGCTTCGT 
    GATGCTCTCG ACACTCAATA CGACTCGGAA TCCAACAAAT TACCGCCGAA AATCAAGGAA 
    ATCCTTCCGC TTCAGGATCA TCCGTTGTCC CATGTCACCC GAGCGTGCCG CAAAGTTTTC 
    ATGCCATTGG ACCTATTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294391.1
    CDS1..4641
    Translation

    Target ORF information:

    RefSeq Version NM_001307462.1
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307462.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGAGTA CGATTAACGC GTTAGCTGAT ACAAGGACGT CTATGGTCTT CATCCCGGCA 
    CCGGAGCCAT TTTTGAATCA GGAGACTGGC GAAGAGCTGC AATTTGTCTA TGGGGAATTC 
    CCGTCGAAAA TGCGAAGCGA GTACTTCCAA CTGGATCGAC TTGAGAAAAA TGGGAAAGAT 
    GGGGAAAAGG TTCCCATGCA TTTTGAAGCA TCCCGTTGCT CGGCAGTTCG CACAAAACCG 
    GAAACCCGTT CCTCGTTGCT AGCCGCCACA AATGCTGTGA AAACCAACCC GCTTCATTGG 
    TCAAAAACGC TTCTGTTCTA CTCATATTCA CTATGGTTCA TGCAATTGGA TAGCCTCCTC 
    ACCGCTGCTC CAAACAAGAA GAAAATACTT CGTCTGGCAT TTAATGTGCT CGATCGAATG 
    GAAAAAACCG AGATTTTCCC GCTCGATCAA GTATGCTATC GAATTCTTAT CGAACTTTGC 
    GGAAAATATT CGGAGCCCGA GATGGCTGTC AAGGTTATGA GAGCCATGCA AAGAGCTGGG 
    CTTGAACAGA ACGCCGTCAC CTATGGAATC TACCACCGCG CCGTTATGGA TGCCACGTGG 
    CCGTCGCCAG CTCGCCAAAA AGCCATAAAA ATGTGGAATT TGCTGAGAAT TGTGCTCACC 
    GGCGCCGAAA CTCTCCGAAA ATGGGCGAAT TTGGAAAGAA GACCGCTGAA AATTGATGAA 
    AAATCGGTGG AAAACACGGA AAATGCTGAG AATGTGCAGG CGGAGATCAC GGTGGATTAT 
    CAGATTACCA GCTATCCACT GGATTCTGTG GAAAATTCGC CGAAAATGAC CCCAAAACGG 
    CACAATTTGG AGCAAAAAGA GCCGCTGATG GATCCACTGG GTGCTCTGGG CCCCCTGGAA 
    GCTGAAAATT TGGAAGTTTC ACGCGAAAAA ACCAAGGAAC GATCAAAATC GCTGGGAAAA 
    GTTGGAATGT CGCCAGCGAG AGCGAAATTC TTTGCCGATC ACGCTACACT GCCATTTTCC 
    AACGAAAATA CGCCGAAATC CGATAAAAAA GAGGCAAAAA GTGGATGGTT TAAGGGAATT 
    GCGAATAGTC CAATGTTTAA AATGATTAAA AGTACTACTT TTGATTCTTC CAAACAAAAC 
    TCTGCTGAAT CATCAAGTGA AACTAGCTCA ATAACCGGAG CTTCTCCATC ATTCCATTCC 
    GTAGTAAATC AATTCAAAAA GGGATACGAT GACGTCATGC CGTCTCGATT CCGTCTCGGC 
    GTTTCCACAC TATTGAACAA ATCCGTCGGT GCAATGGGCT CTGGAATGAT TTATTCGGAC 
    GATCGGGCTT CTGCGGAAAA TCCGTTCTTT GTGAATCCAA AAGATCCGGC TTATGTGCTA 
    GATTCGGGCG TACCGAACTG TCTGCTGCGG GAGGAATATT GGATGCGTGA GGTGTATTTG 
    CAGTTGAAAC GGAGCCGTGA GCGGGTTGAA AAGGGTCAGA AAAGCGAGGA AAACGAGGAG 
    ATTTCCGATG GAATAGCGGA TTTCCCCCAA TTCTCCGCAA AAACTCCAGG AAAACTGGAG 
    GTGATCCTGT GCACCGCCAC CTACTGCCCG TCATGCCGAA CAATGGTCTA CGATGAGGAA 
    ATCATGGCCG GCTGGAAAGT TGACGATCAG AATTTGAACA CAATATGCCC ACACTGCTCG 
    GGAAGCGGAA CGGCGACAGA CGCCGTCTCC AACGAAACCG CAGCCATTCA GGAGAGCGTT 
    TTCGCGCCGA GACTCAAAAT TCGAATGAGA TGGATTGAGG AGATGAAGAG TAGTTGGTAT 
    CAGCCTGGTG GACTGCAGGA GACACCGATT TCGACTCCCG AAAAAACGAC GTCCACTCCC 
    TCGGATATCC AGCTTGAAGT TGCATTTGTA AGCCCATTGG TGCTCCGTCG TGAGCTTGAA 
    ACACTCCTAT CCACTGATAA GGACGCGATG AGGAATGAAT CGCTGAAAAC GACGCATCCG 
    GTGGTTTTCT GGAATTTGAT ATACTATTTG AGAAGACTCT CCCTTCCGAG CCATTTATTC 
    TCGTGGATTG CCGAACGTCA TCACATTCGT TGCGTCTTTG ATCTACCAAC ACAACACGAC 
    GAAACCCGTC TCCCATTGTA CTTTTACAAC CCGAATCACG ATGGAAACGC GCCAAGTTTC 
    GGCGATTTTA CCGAAAAAAT CACCCGAAAA TTGGAGGATG GCTCGGATTC GACGGGCTAT 
    GCTACATGGA AGACGGTCAC CGACTCGGTG AACAAGAATT TGCTGTTTAA GGCAGTTCAG 
    ACACTGGTCA ACTCGTCGCG ATTGGTGAAA AATGGAAGTG TTCATGTTGG AGCACATTTT 
    CCCATTTTTA GAGACATTCA ATTCGCATCG ATTGACGTAT TTGGCAGAGC ACTGCTTCGT 
    GATGCTCTCG ACACTCAATA CGACTCGGAA TCCAACAAAT TACCGCCGAA AATCAAGGAA 
    ATCCTTCCGC TTCAGGATCA TCCGTTGTCC CATGTCACCC GAGCGTGCCG CAAAGTTTTC 
    ATGCCATTGG ACCTATTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    denn-4 ( NM_001307462.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294391.1 Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.
    Price & Availability↑
    CloneID OCa344516
    Clone ID Related Accession (Same CDS sequence) NM_001307462.1 , NM_001307462.2
    Accession Version NM_001307462.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product DENN domain type RAB GEF
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307462.1. COMPLETENESS: incomplete on the 5' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGAGTA CGATTAACGC GTTAGCTGAT ACAAGGACGT CTATGGTCTT CATCCCGGCA 
    CCGGAGCCAT TTTTGAATCA GGAGACTGGC GAAGAGCTGC AATTTGTCTA TGGGGAATTC 
    CCGTCGAAAA TGCGAAGCGA GTACTTCCAA CTGGATCGAC TTGAGAAAAA TGGGAAAGAT 
    GGGGAAAAGG TTCCCATGCA TTTTGAAGCA TCCCGTTGCT CGGCAGTTCG CACAAAACCG 
    GAAACCCGTT CCTCGTTGCT AGCCGCCACA AATGCTGTGA AAACCAACCC GCTTCATTGG 
    TCAAAAACGC TTCTGTTCTA CTCATATTCA CTATGGTTCA TGCAATTGGA TAGCCTCCTC 
    ACCGCTGCTC CAAACAAGAA GAAAATACTT CGTCTGGCAT TTAATGTGCT CGATCGAATG 
    GAAAAAACCG AGATTTTCCC GCTCGATCAA GTATGCTATC GAATTCTTAT CGAACTTTGC 
    GGAAAATATT CGGAGCCCGA GATGGCTGTC AAGGTTATGA GAGCCATGCA AAGAGCTGGG 
    CTTGAACAGA ACGCCGTCAC CTATGGAATC TACCACCGCG CCGTTATGGA TGCCACGTGG 
    CCGTCGCCAG CTCGCCAAAA AGCCATAAAA ATGTGGAATT TGCTGAGAAT TGTGCTCACC 
    GGCGCCGAAA CTCTCCGAAA ATGGGCGAAT TTGGAAAGAA GACCGCTGAA AATTGATGAA 
    AAATCGGTGG AAAACACGGA AAATGCTGAG AATGTGCAGG CGGAGATCAC GGTGGATTAT 
    CAGATTACCA GCTATCCACT GGATTCTGTG GAAAATTCGC CGAAAATGAC CCCAAAACGG 
    CACAATTTGG AGCAAAAAGA GCCGCTGATG GATCCACTGG GTGCTCTGGG CCCCCTGGAA 
    GCTGAAAATT TGGAAGTTTC ACGCGAAAAA ACCAAGGAAC GATCAAAATC GCTGGGAAAA 
    GTTGGAATGT CGCCAGCGAG AGCGAAATTC TTTGCCGATC ACGCTACACT GCCATTTTCC 
    AACGAAAATA CGCCGAAATC CGATAAAAAA GAGGCAAAAA GTGGATGGTT TAAGGGAATT 
    GCGAATAGTC CAATGTTTAA AATGATTAAA AGTACTACTT TTGATTCTTC CAAACAAAAC 
    TCTGCTGAAT CATCAAGTGA AACTAGCTCA ATAACCGGAG CTTCTCCATC ATTCCATTCC 
    GTAGTAAATC AATTCAAAAA GGGATACGAT GACGTCATGC CGTCTCGATT CCGTCTCGGC 
    GTTTCCACAC TATTGAACAA ATCCGTCGGT GCAATGGGCT CTGGAATGAT TTATTCGGAC 
    GATCGGGCTT CTGCGGAAAA TCCGTTCTTT GTGAATCCAA AAGATCCGGC TTATGTGCTA 
    GATTCGGGCG TACCGAACTG TCTGCTGCGG GAGGAATATT GGATGCGTGA GGTGTATTTG 
    CAGTTGAAAC GGAGCCGTGA GCGGGTTGAA AAGGGTCAGA AAAGCGAGGA AAACGAGGAG 
    ATTTCCGATG GAATAGCGGA TTTCCCCCAA TTCTCCGCAA AAACTCCAGG AAAACTGGAG 
    GTGATCCTGT GCACCGCCAC CTACTGCCCG TCATGCCGAA CAATGGTCTA CGATGAGGAA 
    ATCATGGCCG GCTGGAAAGT TGACGATCAG AATTTGAACA CAATATGCCC ACACTGCTCG 
    GGAAGCGGAA CGGCGACAGA CGCCGTCTCC AACGAAACCG CAGCCATTCA GGAGAGCGTT 
    TTCGCGCCGA GACTCAAAAT TCGAATGAGA TGGATTGAGG AGATGAAGAG TAGTTGGTAT 
    CAGCCTGGTG GACTGCAGGA GACACCGATT TCGACTCCCG AAAAAACGAC GTCCACTCCC 
    TCGGATATCC AGCTTGAAGT TGCATTTGTA AGCCCATTGG TGCTCCGTCG TGAGCTTGAA 
    ACACTCCTAT CCACTGATAA GGACGCGATG AGGAATGAAT CGCTGAAAAC GACGCATCCG 
    GTGGTTTTCT GGAATTTGAT ATACTATTTG AGAAGACTCT CCCTTCCGAG CCATTTATTC 
    TCGTGGATTG CCGAACGTCA TCACATTCGT TGCGTCTTTG ATCTACCAAC ACAACACGAC 
    GAAACCCGTC TCCCATTGTA CTTTTACAAC CCGAATCACG ATGGAAACGC GCCAAGTTTC 
    GGCGATTTTA CCGAAAAAAT CACCCGAAAA TTGGAGGATG GCTCGGATTC GACGGGCTAT 
    GCTACATGGA AGACGGTCAC CGACTCGGTG AACAAGAATT TGCTGTTTAA GGCAGTTCAG 
    ACACTGGTCA ACTCGTCGCG ATTGGTGAAA AATGGAAGTG TTCATGTTGG AGCACATTTT 
    CCCATTTTTA GAGACATTCA ATTCGCATCG ATTGACGTAT TTGGCAGAGC ACTGCTTCGT 
    GATGCTCTCG ACACTCAATA CGACTCGGAA TCCAACAAAT TACCGCCGAA AATCAAGGAA 
    ATCCTTCCGC TTCAGGATCA TCCGTTGTCC CATGTCACCC GAGCGTGCCG CAAAGTTTTC 
    ATGCCATTGG ACCTATTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001294391.1
    CDS1..4641
    Translation

    Target ORF information:

    RefSeq Version NM_001307462.2
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans DENN domain type RAB GEF (denn-4), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001307462.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTAGTG GTGCCGGGTT AACCGGATCA CACGATGAAC GACGACTTTT CGAACATTTT 
    GTCATAGCCG GTTTGGCCGA AAATCAGCCG CTGGAACGTC TAAAACCGTC GTCCGAGGAA 
    TGTGGGCTCC GAAATACGCA ACCACTGGCT CCGATCACCG ACATTGCCGT CATTTTCCCG 
    AGTCTCGGTG AAAAACCGCC CGATGGTTTC GACGTTATCG AATCGACTAC ACTGGGCTAC 
    CCGGCAGACC TGAATCACGG CTCCATAGGC CAAAAATCAG TGTTTTTATG CTACAAAAGA 
    GGTTATAACA AGCTTCCACT AGTTGATATT GGAATTTTGG ACGAAGGACG TGGTGATAAG 
    CCAATGATTG ATTCGAATGT AGTTCAGACG ACACCGTTTG GGCGGTGTGC AAGTGTTAAT 
    AACGCTTCAC AGGGAATCTA CCTCACCTAT CGACGAGCTC CACCAAACTC TTCACAGAGC 
    CAATTCGTAG TGACAGATAT CAAAGTTATT CTGGCGAATA AGGGAGAAAT CCCACCGCAC 
    ACCTACTACA AAATCACCAA AAACCTGAAT AAAGGTCTCG TCGGTTCGGA TGTCTTCATT 
    TGTTATAAGA AATCACAGGG AACAGCGAAA CGGTTGGCCT ATAAGCCGGC AGTTTTGGAT 
    TATTTCCCCC GAAGTGATCA TTCGGGTGAA ATGGAAGATT TTAAGCTCGC ACAGAACGTT 
    GCAATGTTCT GCCTACCGAT GGGAGCACTT ATCGAGTGTT GGCCTACGAA ATGTGCACCT 
    CCTGATCGCA GTTTTTCCAC TTTTGTGCTC ACTGATGAGA ATGGTACAAA ATTCTACGGC 
    TCCGCAGTAA CGTTTTACGA AAAATATACA AAACAGTTGA GCGAAGATCA ATTGGAACAA 
    TTGGACCTGA AAAGTGACGA AGGTGGAACT GTGGAGGATT CTGGCAGTAA TAATGATCCA 
    GCTGATGATT TGCAGATTCT CTTCTACAAA AATGTGTCAA TATGTCTGAT ATCGCGTTTT 
    CCGTTCTTCA ACTCATTCAA ACGATTTCTC TTCTTTCTAC ACAGAATGTC ATCGACACCG 
    AGTTGTTCAC ATCCAGTGCC AATTGAACGA TACATTTCTC ATTTGATGTA CGAAGTTTCG 
    TTCCCAACAC CCCGCCGCCC ACGCGTTTTC ATGCAACTTG GCGCTGAAAA TATCAGTTTT 
    GACTCACATG ATGATAGTCA ACTCCCGTTG AACGGAGCTC AACTAATCGA TACTCTAAAA 
    TATCTTGGAA GTGAAAATCT CATGTATTTG ATGCTTCTGG CGCTGCTCGA GCAGAAAATT 
    TTGATTCATA GTTTACGACC ATGGATGCTT GCCGCCGTAG CCGAATCAGT GTGTGCTCTG 
    ATGTTCCCAT TCCATTGGCA ATGCCCATAC ATCCCACAAT GTCCGTTGGG ACTTGCCGGC 
    GTACTCCATG CCCCGCTTCC ATTCATCGCC GGCGTCGATT CCCGATACTT TGAGCTTTAC 
    GAAGATCCAC CTGATGACGT CACCTGCTTC GATTTGGACA CAAGTACGAT CAGTTTTTCG 
    ACAGTCCGCG CCACTTTCAA AACGTCGATG TTGCCGAAAA AACCGGCAAA ACAGCTGAAA 
    TCGGCGTTGG AGGATATATT TGGAAAGCTG CAGAAACAGG ATTATTCGAC AAATTCAAAA 
    AGTGATGGAG GATTTATTCC AGTAGATCAG GATTTGGTAA AACAGCGACA ACGACGAGAG 
    TTCGAGAATG AAATTCACGA TGCTTTTTTG AAATTCATCG CGAGTATTAT GAAGGGATAT 
    CAAGCGTTTT TAAGGCCTAT CAAGGGGGCT CCAATGACTG AACATGCTAC AGATACTGGG 
    AATTTGTTCG ATTTGGATGG GTTTTTAAGA TCCCGTGACC GTTCGTCTGC AGACTTTTAC 
    AAACGATTCT CGGAGACGCA GTCTTTTATG AGATTTATAG AGGAACGAAG CTTTGTATCG 
    GATAAAAACA CATATAATGC ATTCTTCGAT GATTGTATTG CAAAGTTGAC GAGCGCAGTG 
    GAAGACGGTC GAGATATCAG TGGAGTTCAA CTTTTAGAGC CCGACACATC GCACAGCCAC 
    ACTACGAGTA CGATTAACGC GTTAGCTGAT ACAAGGACGT CTATGGTCTT CATCCCGGCA 
    CCGGAGCCAT TTTTGAATCA GGAGACTGGC GAAGAGCTGC AATTTGTCTA TGGGGAATTC 
    CCGTCGAAAA TGCGAAGCGA GTACTTCCAA CTGGATCGAC TTGAGAAAAA TGGGAAAGAT 
    GGGGAAAAGG TTCCCATGCA TTTTGAAGCA TCCCGTTGCT CGGCAGTTCG CACAAAACCG 
    GAAACCCGTT CCTCGTTGCT AGCCGCCACA AATGCTGTGA AAACCAACCC GCTTCATTGG 
    TCAAAAACGC TTCTGTTCTA CTCATATTCA CTATGGTTCA TGCAATTGGA TAGCCTCCTC 
    ACCGCTGCTC CAAACAAGAA GAAAATACTT CGTCTGGCAT TTAATGTGCT CGATCGAATG 
    GAAAAAACCG AGATTTTCCC GCTCGATCAA GTATGCTATC GAATTCTTAT CGAACTTTGC 
    GGAAAATATT CGGAGCCCGA GATGGCTGTC AAGGTTATGA GAGCCATGCA AAGAGCTGGG 
    CTTGAACAGA ACGCCGTCAC CTATGGAATC TACCACCGCG CCGTTATGGA TGCCACGTGG 
    CCGTCGCCAG CTCGCCAAAA AGCCATAAAA ATGTGGAATT TGCTGAGAAT TGTGCTCACC 
    GGCGCCGAAA CTCTCCGAAA ATGGGCGAAT TTGGAAAGAA GACCGCTGAA AATTGATGAA 
    AAATCGGTGG AAAACACGGA AAATGCTGAG AATGTGCAGG CGGAGATCAC GGTGGATTAT 
    CAGATTACCA GCTATCCACT GGATTCTGTG GAAAATTCGC CGAAAATGAC CCCAAAACGG 
    CACAATTTGG AGCAAAAAGA GCCGCTGATG GATCCACTGG GTGCTCTGGG CCCCCTGGAA 
    GCTGAAAATT TGGAAGTTTC ACGCGAAAAA ACCAAGGAAC GATCAAAATC GCTGGGAAAA 
    GTTGGAATGT CGCCAGCGAG AGCGAAATTC TTTGCCGATC ACGCTACACT GCCATTTTCC 
    AACGAAAATA CGCCGAAATC CGATAAAAAA GAGGCAAAAA GTGGATGGTT TAAGGGAATT 
    GCGAATAGTC CAATGTTTAA AATGATTAAA AGTACTACTT TTGATTCTTC CAAACAAAAC 
    TCTGCTGAAT CATCAAGTGA AACTAGCTCA ATAACCGGAG CTTCTCCATC ATTCCATTCC 
    GTAGTAAATC AATTCAAAAA GGGATACGAT GACGTCATGC CGTCTCGATT CCGTCTCGGC 
    GTTTCCACAC TATTGAACAA ATCCGTCGGT GCAATGGGCT CTGGAATGAT TTATTCGGAC 
    GATCGGGCTT CTGCGGAAAA TCCGTTCTTT GTGAATCCAA AAGATCCGGC TTATGTGCTA 
    GATTCGGGCG TACCGAACTG TCTGCTGCGG GAGGAATATT GGATGCGTGA GGTGTATTTG 
    CAGTTGAAAC GGAGCCGTGA GCGGGTTGAA AAGGGTCAGA AAAGCGAGGA AAACGAGGAG 
    ATTTCCGATG GAATAGCGGA TTTCCCCCAA TTCTCCGCAA AAACTCCAGG AAAACTGGAG 
    GTGATCCTGT GCACCGCCAC CTACTGCCCG TCATGCCGAA CAATGGTCTA CGATGAGGAA 
    ATCATGGCCG GCTGGAAAGT TGACGATCAG AATTTGAACA CAATATGCCC ACACTGCTCG 
    GGAAGCGGAA CGGCGACAGA CGCCGTCTCC AACGAAACCG CAGCCATTCA GGAGAGCGTT 
    TTCGCGCCGA GACTCAAAAT TCGAATGAGA TGGATTGAGG AGATGAAGAG TAGTTGGTAT 
    CAGCCTGGTG GACTGCAGGA GACACCGATT TCGACTCCCG AAAAAACGAC GTCCACTCCC 
    TCGGATATCC AGCTTGAAGT TGCATTTGTA AGCCCATTGG TGCTCCGTCG TGAGCTTGAA 
    ACACTCCTAT CCACTGATAA GGACGCGATG AGGAATGAAT CGCTGAAAAC GACGCATCCG 
    GTGGTTTTCT GGAATTTGAT ATACTATTTG AGAAGACTCT CCCTTCCGAG CCATTTATTC 
    TCGTGGATTG CCGAACGTCA TCACATTCGT TGCGTCTTTG ATCTACCAAC ACAACACGAC 
    GAAACCCGTC TCCCATTGTA CTTTTACAAC CCGAATCACG ATGGAAACGC GCCAAGTTTC 
    GGCGATTTTA CCGAAAAAAT CACCCGAAAA TTGGAGGATG GCTCGGATTC GACGGGCTAT 
    GCTACATGGA AGACGGTCAC CGACTCGGTG AACAAGAATT TGCTGTTTAA GGCAGTTCAG 
    ACACTGGTCA ACTCGTCGCG ATTGGTGAAA AATGGAAGTG TTCATGTTGG AGCACATTTT 
    CCCATTTTTA GAGACATTCA ATTCGCATCG ATTGACGTAT TTGGCAGAGC ACTGCTTCGT 
    GATGCTCTCG ACACTCAATA CGACTCGGAA TCCAACAAAT TACCGCCGAA AATCAAGGAA 
    ATCCTTCCGC TTCAGGATCA TCCGTTGTCC CATGTCACCC GAGCGTGCCG CAAAGTTTTC 
    ATGCCATTGG ACCTATTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

見積依頼

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