pek-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	pek-1
Entrez Gene ID	181334
Full Name	Eukaryotic translation initiation factor 2-alpha kinase pek-1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_077511.6	NP_509912.1	Eukaryotic translation initiation factor 2-alpha kinase pek-1
NM_077511.7	NP_509912.1	Eukaryotic translation initiation factor 2-alpha kinase pek-1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In conclusion, we identified PEK-1 as a mediator of hormesis in the mev-1 mutant of C. elegans which is induced by simultaneous inhibition of XBP-1 and its splicing activator IRE-1 and mediated through activation of the proteasome.
Title: PEK-1 is crucial for hormesis induced by inhibition of the IRE-1/XBP-1 pathway in the Caenorhabditis elegans mev-1 mutant.

Our data suggest that the negative feedback loops involving the activation of IRE-1-XBP-1 and PEK-1 pathways serve essential roles, not only at the extremes of ER stress, but also in the maintenance of ER homeostasis under physiological conditions.
Title: Physiological IRE-1-XBP-1 and PEK-1 signaling in Caenorhabditis elegans larval development and immunity.

the salt bridge interaction and dimer interface observed in the PKR structure is critical for the activity of PKR, GCN2, and PERK
Title: Conserved intermolecular salt bridge required for activation of protein kinases PKR, GCN2, and PERK.

The following pek-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pek-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa350155	NM_077511.7 Latest version!	Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase pek-1 (pek-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
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OCa350155	NM_077511.6	Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase pek-1 (pek-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pek-1 ( NM_077511.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_509912.1 Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase pek-1 (pek-1), partial mRNA.

Price & Availability↑

CloneID	OCa350155
Clone ID Related Accession (Same CDS sequence)	NM_077511.6 , NM_077511.7
Accession Version	NM_077511.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Eukaryotic translation initiation factor 2-alpha kinase pek-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_077511.5. COMPLETENESS: incomplete on both ends.

ATGAGTGTGT ATTATATAGT TTTAGCTGGG TTCTTGCTCT TCATGGCCTT GGTACCATTC 
AACGCTGGCC AACAGTACAT CGATGATGAC ATAGAAGTTG TATCATCTTG TCAAAATGGT 
TATGTACAAA ATGCGGGTTG TGATCAGAAT AGTCAAGTGA ACATTATTAT CACCGCTACT 
CTGGATGGAG TCGTCACTGC ACTGGATGGA GAAACGGGTG AAATGATTTG GAGATACGAG 
GATGCACCAC TTTTAAGAGG AACGCTGAGC ACATCTGACC CGATTGACAT TGGAGGAACC 
AGTTTACAAC TTATGCCAAC ATTAGATGGA AGATTGTTTT CGTACACACA TAACACAAAT 
CTCATTGAAC CACTCCCAAT CACTACTGAT TCTCTTCTGG AATCTACTAT CCGCCTTGGA 
CAGGACGCCG TAGCTGGTGG AAAAAGTGTT ACTACCAAAG GTTTTGACTT ATTCACAGGA 
GAGCAAAAAT ATGAGTGTTC AATGGAGTCT TGTGGACCCG GAGATACGGA AACTCCAAAA 
AATCCAATAA TTTTAATAAG GCGGACTACG AACTCGATTC GTGCAATGGA CACTCTTCGT 
GGAATTGAAA GATGGAACTT ATCTACAGCT GAGATTGGTG TCACTTTGGC TGGCGGAATA 
ACTAGTCCAA CATTAGTTTC TGACGTCAAA ATATTGCTCC AACCCCCAGA CGGAGTGATT 
GTTGCTGTTG ACAAGTACAA CCGCGAGGAA TGGAAAACGA ACGTAGATGG GCACATTGTT 
AGTGTTTGGC AAGTTTATGG AAATCAAATC GGAGAGATAT CCATCTTCGA CCCAAGTAAT 
ATTTTCACCA CTCAATATGA AGTGATGCAG CGTGAACAAC ACAACTTGCA AACTCAGTCA 
TCTCTCCTTT ACATGGGTAC ATCAAATGGA TTTCCATTCA TCATTCAAAG TCCCAAAGCT 
AAGAATAACT TGAAGCAGAG AATGAACGCA TTGCCAGAGC TTTCAACAAT GACAGAGCTC 
ACGAATCCTC GATTCTGCAC TGCTAATGAA GAAACCAGAA GTCTTGCATA CAATGTGAAG 
GATGAAACCT TGCGATTGGT GCTTCATAAT GCGTTTCGTC ATTCCCAAAG CAAAGCCATT 
GAAGACAAGT CATTAAGTGG CTCTTCTGCT AGACGGAAGT TACAAATCAT TGCTTCGGAT 
ACTGAAGTAT CAGCACAGCG GATTGGAACT GAAAACTTGA GATCTACTTC GGTGTCAAAA 
TCAGGTGATT ACGGTTATCT TGTTTTGGAA TCCGAACCAC AAAGAGTCAA ATTCAAAGTT 
AATTCTCCAA TCACCCTTAT GCAAACGATC TTCAGTTATA TTTTCAACCC GACCGCTGTT 
GTCAGTTTTC TTGCTGGACT AATTGGTGTT ACTGTAGCGG TGGTATACAA TAAAATCGCA 
AAAAGTTCTC CTCGAATGAT TGAACACCTA TCAAGCACCG AAAGTGCAGA AACTGAAAGT 
GCATCCCACA GAACTCGAAC TACGTCGTTT GCACCGACAG ATGATGAAAT TGAACGATTT 
GTTGAAGAAG GATCAGATTT GAGTACCACA CCAATTGGAG CAATACATAG GAAACCGCTA 
ATGCCCATTG AAAAGTCAAA CATTGAGACT CATACTCAAC CTCAAATAAA GCCCGTTCAA 
CGACTGGTAA AAACTGACAT TGACACCGAC GAGGATTCAT TTTCAAATGA TGAGAAAAAG 
CGACTTTTGA GAAATCGGAC GATTTCACGC TCAAGTCTCG AAGGATTCAC AAGTAGATTT 
GCCAACGAAT TTGAGGTCAA GAAGGTCATC GGGCATGGTG GTTTTGGTGT TGTGTTCCGT 
GCTCAAAGTA TTACAGACAT GAACGAGTAC GCTGTCAAGA GAATTGCGGT TGCTGATAAT 
GACAAAGCGA GAAATCGTGT GCTCCGAGAA GCAAGAGCTC TTGCGATGTT TGATCATCCT 
GGAATTATTC GGTACTTCTA TGCTTGGGAG GAAAGGCCGC CGAAGGGATT TCAGGAAAAA 
GAAGATGAGA ATCTTCTTGG GAAAATAAAA GCTGAAAAAC TGGCAAAACT TCATGAAATC 
AAAAAAGCAA AGAAGCACAC AAGTGAAGGG AAACGTGTCA GATCAGCAGA CACTGCATCT 
TTTGCGGAGT CATTTGCAAT GCCTCCCGTT GTTGGAAATA CAACTGATGC TGAGAATAGT 
TGGAGTACGT CTGCAAAGCC ACAAGAAGTC GGAGCAAAAC GCACAACTTC CGAAAGCAAG 
CTTGGATTGC ATGGAGGATC TGACAGAACT ACTGCCGAGC TGAAAGAAGA GAGCGTTGCC 
TTCTCAGAAA GTGATGAAGA ATCGGACACT ACAGAGGATA GTAGCTCTAG TGATGAATCA 
CCTTCCTCAT CTTCCGGCTC TTCTATTGAT GATGAGCCCA AGAAGTATAA CTCTTCATCA 
GGAGGAATTG AGTTTGTCGA TGGCTCAGAT GATGTTGATA ATGAAGCTGT GAAAGAAACA 
AAGAAGGAGA TTGCTGTGAT TGAAGAACTC TCAATCCATA ACAGAGCGAT GATCGTTGAG 
ACGGAAAATC AAGAACTGGA GGTTCGAGAA AGAAATGATA CTGGTGACTG TGCCTATTTG 
TATATTGTAA TGCAATTATG TGCCGAGAAG ACCCTTGAAG ATTGGATCCG CCGCAGCAAA 
ACCATGGAAA GTCGTCCGCT ATTCACGATG AAAAATTGGA TTAAACAATT GGCGAGCGGT 
CTTGAATACC TTCACAACAA AGGATTCATC CACAGAGATT TGAAACCAGG AAATGTATTT 
TTCTCTCTCG ACTCAACACA TGGTCATCAA ATCCTCAAAA TTGGAGATCT TGGATTGGCA 
ACTAAAACTG ATGGAGCTCC CAAAATAACA GTTCGCCAGG ATTCAGACAG CAGTGCAAAG 
CATACGAAAA ACGTCGGCAC TCGATCCTAC ATGTCTCCAG AGCAATTGAA ACATCAACAA 
TACACGGAGA AAGTGGATAT TTTCGCACTG GGACTAGTCG CAACAGAGCT CATCATTTCA 
TTTTCTACTG CTAGCGAGAG AATTCACACA TTTGCTGATT TCCAGAAAGG AGATATTCCA 
GCCATTTTGG ACAATGTGCC AGAGTCGCGG GATTTCCTTC TCCAACTGAC CAGCTTGGAA 
CCATCAGAAC GTCCTACTGC CCATGAAGTA GCTACTCATA AATTTCTCCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_509912.1
CDS	1..3234
Translation	MSVYYIVLAGFLLFMALVPFNAGQQYIDDDIEVVSSCQNGYVQNAGCDQNSQVNIIITATLDGVVTALDGETGEMIWRYEDAPLLRGTLSTSDPIDIGGTSLQLMPTLDGRLFSYTHNTNLIEPLPITTDSLLESTIRLGQDAVAGGKSVTTKGFDLFTGEQKYECSMESCGPGDTETPKNPIILIRRTTNSIRAMDTLRGIERWNLSTAEIGVTLAGGITSPTLVSDVKILLQPPDGVIVAVDKYNREEWKTNVDGHIVSVWQVYGNQIGEISIFDPSNIFTTQYEVMQREQHNLQTQSSLLYMGTSNGFPFIIQSPKAKNNLKQRMNALPELSTMTELTNPRFCTANEETRSLAYNVKDETLRLVLHNAFRHSQSKAIEDKSLSGSSARRKLQIIASDTEVSAQRIGTENLRSTSVSKSGDYGYLVLESEPQRVKFKVNSPITLMQTIFSYIFNPTAVVSFLAGLIGVTVAVVYNKIAKSSPRMIEHLSSTESAETESASHRTRTTSFAPTDDEIERFVEEGSDLSTTPIGAIHRKPLMPIEKSNIETHTQPQIKPVQRLVKTDIDTDEDSFSNDEKKRLLRNRTISRSSLEGFTSRFANEFEVKKVIGHGGFGVVFRAQSITDMNEYAVKRIAVADNDKARNRVLREARALAMFDHPGIIRYFYAWEERPPKGFQEKEDENLLGKIKAEKLAKLHEIKKAKKHTSEGKRVRSADTASFAESFAMPPVVGNTTDAENSWSTSAKPQEVGAKRTTSESKLGLHGGSDRTTAELKEESVAFSESDEESDTTEDSSSSDESPSSSSGSSIDDEPKKYNSSSGGIEFVDGSDDVDNEAVKETKKEIAVIEELSIHNRAMIVETENQELEVRERNDTGDCAYLYIVMQLCAEKTLEDWIRRSKTMESRPLFTMKNWIKQLASGLEYLHNKGFIHRDLKPGNVFFSLDSTHGHQILKIGDLGLATKTDGAPKITVRQDSDSSAKHTKNVGTRSYMSPEQLKHQQYTEKVDIFALGLVATELIISFSTASERIHTFADFQKGDIPAILDNVPESRDFLLQLTSLEPSERPTAHEVATHKFLQ

Target ORF information:

RefSeq Version	NM_077511.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase pek-1 (pek-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_077511.6

ORF Insert Sequence:

ATGAGTGTGT ATTATATAGT TTTAGCTGGG TTCTTGCTCT TCATGGCCTT GGTACCATTC 
AACGCTGGCC AACAGTACAT CGATGATGAC ATAGAAGTTG TATCATCTTG TCAAAATGGT 
TATGTACAAA ATGCGGGTTG TGATCAGAAT AGTCAAGTGA ACATTATTAT CACCGCTACT 
CTGGATGGAG TCGTCACTGC ACTGGATGGA GAAACGGGTG AAATGATTTG GAGATACGAG 
GATGCACCAC TTTTAAGAGG AACGCTGAGC ACATCTGACC CGATTGACAT TGGAGGAACC 
AGTTTACAAC TTATGCCAAC ATTAGATGGA AGATTGTTTT CGTACACACA TAACACAAAT 
CTCATTGAAC CACTCCCAAT CACTACTGAT TCTCTTCTGG AATCTACTAT CCGCCTTGGA 
CAGGACGCCG TAGCTGGTGG AAAAAGTGTT ACTACCAAAG GTTTTGACTT ATTCACAGGA 
GAGCAAAAAT ATGAGTGTTC AATGGAGTCT TGTGGACCCG GAGATACGGA AACTCCAAAA 
AATCCAATAA TTTTAATAAG GCGGACTACG AACTCGATTC GTGCAATGGA CACTCTTCGT 
GGAATTGAAA GATGGAACTT ATCTACAGCT GAGATTGGTG TCACTTTGGC TGGCGGAATA 
ACTAGTCCAA CATTAGTTTC TGACGTCAAA ATATTGCTCC AACCCCCAGA CGGAGTGATT 
GTTGCTGTTG ACAAGTACAA CCGCGAGGAA TGGAAAACGA ACGTAGATGG GCACATTGTT 
AGTGTTTGGC AAGTTTATGG AAATCAAATC GGAGAGATAT CCATCTTCGA CCCAAGTAAT 
ATTTTCACCA CTCAATATGA AGTGATGCAG CGTGAACAAC ACAACTTGCA AACTCAGTCA 
TCTCTCCTTT ACATGGGTAC ATCAAATGGA TTTCCATTCA TCATTCAAAG TCCCAAAGCT 
AAGAATAACT TGAAGCAGAG AATGAACGCA TTGCCAGAGC TTTCAACAAT GACAGAGCTC 
ACGAATCCTC GATTCTGCAC TGCTAATGAA GAAACCAGAA GTCTTGCATA CAATGTGAAG 
GATGAAACCT TGCGATTGGT GCTTCATAAT GCGTTTCGTC ATTCCCAAAG CAAAGCCATT 
GAAGACAAGT CATTAAGTGG CTCTTCTGCT AGACGGAAGT TACAAATCAT TGCTTCGGAT 
ACTGAAGTAT CAGCACAGCG GATTGGAACT GAAAACTTGA GATCTACTTC GGTGTCAAAA 
TCAGGTGATT ACGGTTATCT TGTTTTGGAA TCCGAACCAC AAAGAGTCAA ATTCAAAGTT 
AATTCTCCAA TCACCCTTAT GCAAACGATC TTCAGTTATA TTTTCAACCC GACCGCTGTT 
GTCAGTTTTC TTGCTGGACT AATTGGTGTT ACTGTAGCGG TGGTATACAA TAAAATCGCA 
AAAAGTTCTC CTCGAATGAT TGAACACCTA TCAAGCACCG AAAGTGCAGA AACTGAAAGT 
GCATCCCACA GAACTCGAAC TACGTCGTTT GCACCGACAG ATGATGAAAT TGAACGATTT 
GTTGAAGAAG GATCAGATTT GAGTACCACA CCAATTGGAG CAATACATAG GAAACCGCTA 
ATGCCCATTG AAAAGTCAAA CATTGAGACT CATACTCAAC CTCAAATAAA GCCCGTTCAA 
CGACTGGTAA AAACTGACAT TGACACCGAC GAGGATTCAT TTTCAAATGA TGAGAAAAAG 
CGACTTTTGA GAAATCGGAC GATTTCACGC TCAAGTCTCG AAGGATTCAC AAGTAGATTT 
GCCAACGAAT TTGAGGTCAA GAAGGTCATC GGGCATGGTG GTTTTGGTGT TGTGTTCCGT 
GCTCAAAGTA TTACAGACAT GAACGAGTAC GCTGTCAAGA GAATTGCGGT TGCTGATAAT 
GACAAAGCGA GAAATCGTGT GCTCCGAGAA GCAAGAGCTC TTGCGATGTT TGATCATCCT 
GGAATTATTC GGTACTTCTA TGCTTGGGAG GAAAGGCCGC CGAAGGGATT TCAGGAAAAA 
GAAGATGAGA ATCTTCTTGG GAAAATAAAA GCTGAAAAAC TGGCAAAACT TCATGAAATC 
AAAAAAGCAA AGAAGCACAC AAGTGAAGGG AAACGTGTCA GATCAGCAGA CACTGCATCT 
TTTGCGGAGT CATTTGCAAT GCCTCCCGTT GTTGGAAATA CAACTGATGC TGAGAATAGT 
TGGAGTACGT CTGCAAAGCC ACAAGAAGTC GGAGCAAAAC GCACAACTTC CGAAAGCAAG 
CTTGGATTGC ATGGAGGATC TGACAGAACT ACTGCCGAGC TGAAAGAAGA GAGCGTTGCC 
TTCTCAGAAA GTGATGAAGA ATCGGACACT ACAGAGGATA GTAGCTCTAG TGATGAATCA 
CCTTCCTCAT CTTCCGGCTC TTCTATTGAT GATGAGCCCA AGAAGTATAA CTCTTCATCA 
GGAGGAATTG AGTTTGTCGA TGGCTCAGAT GATGTTGATA ATGAAGCTGT GAAAGAAACA 
AAGAAGGAGA TTGCTGTGAT TGAAGAACTC TCAATCCATA ACAGAGCGAT GATCGTTGAG 
ACGGAAAATC AAGAACTGGA GGTTCGAGAA AGAAATGATA CTGGTGACTG TGCCTATTTG 
TATATTGTAA TGCAATTATG TGCCGAGAAG ACCCTTGAAG ATTGGATCCG CCGCAGCAAA 
ACCATGGAAA GTCGTCCGCT ATTCACGATG AAAAATTGGA TTAAACAATT GGCGAGCGGT 
CTTGAATACC TTCACAACAA AGGATTCATC CACAGAGATT TGAAACCAGG AAATGTATTT 
TTCTCTCTCG ACTCAACACA TGGTCATCAA ATCCTCAAAA TTGGAGATCT TGGATTGGCA 
ACTAAAACTG ATGGAGCTCC CAAAATAACA GTTCGCCAGG ATTCAGACAG CAGTGCAAAG 
CATACGAAAA ACGTCGGCAC TCGATCCTAC ATGTCTCCAG AGCAATTGAA ACATCAACAA 
TACACGGAGA AAGTGGATAT TTTCGCACTG GGACTAGTCG CAACAGAGCT CATCATTTCA 
TTTTCTACTG CTAGCGAGAG AATTCACACA TTTGCTGATT TCCAGAAAGG AGATATTCCA 
GCCATTTTGG ACAATGTGCC AGAGTCGCGG GATTTCCTTC TCCAACTGAC CAGCTTGGAA 
CCATCAGAAC GTCCTACTGC CCATGAAGTA GCTACTCATA AATTTCTCCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pek-1 ( NM_077511.7 ) cDNA ORF clone, Caenorhabditis elegans -> NP_509912.1 Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase pek-1 (pek-1), mRNA.

Price & Availability↑

CloneID	OCa350155
Clone ID Related Accession (Same CDS sequence)	NM_077511.6 , NM_077511.7
Accession Version	NM_077511.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Eukaryotic translation initiation factor 2-alpha kinase pek-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_077511.6.

ATGAGTGTGT ATTATATAGT TTTAGCTGGG TTCTTGCTCT TCATGGCCTT GGTACCATTC 
AACGCTGGCC AACAGTACAT CGATGATGAC ATAGAAGTTG TATCATCTTG TCAAAATGGT 
TATGTACAAA ATGCGGGTTG TGATCAGAAT AGTCAAGTGA ACATTATTAT CACCGCTACT 
CTGGATGGAG TCGTCACTGC ACTGGATGGA GAAACGGGTG AAATGATTTG GAGATACGAG 
GATGCACCAC TTTTAAGAGG AACGCTGAGC ACATCTGACC CGATTGACAT TGGAGGAACC 
AGTTTACAAC TTATGCCAAC ATTAGATGGA AGATTGTTTT CGTACACACA TAACACAAAT 
CTCATTGAAC CACTCCCAAT CACTACTGAT TCTCTTCTGG AATCTACTAT CCGCCTTGGA 
CAGGACGCCG TAGCTGGTGG AAAAAGTGTT ACTACCAAAG GTTTTGACTT ATTCACAGGA 
GAGCAAAAAT ATGAGTGTTC AATGGAGTCT TGTGGACCCG GAGATACGGA AACTCCAAAA 
AATCCAATAA TTTTAATAAG GCGGACTACG AACTCGATTC GTGCAATGGA CACTCTTCGT 
GGAATTGAAA GATGGAACTT ATCTACAGCT GAGATTGGTG TCACTTTGGC TGGCGGAATA 
ACTAGTCCAA CATTAGTTTC TGACGTCAAA ATATTGCTCC AACCCCCAGA CGGAGTGATT 
GTTGCTGTTG ACAAGTACAA CCGCGAGGAA TGGAAAACGA ACGTAGATGG GCACATTGTT 
AGTGTTTGGC AAGTTTATGG AAATCAAATC GGAGAGATAT CCATCTTCGA CCCAAGTAAT 
ATTTTCACCA CTCAATATGA AGTGATGCAG CGTGAACAAC ACAACTTGCA AACTCAGTCA 
TCTCTCCTTT ACATGGGTAC ATCAAATGGA TTTCCATTCA TCATTCAAAG TCCCAAAGCT 
AAGAATAACT TGAAGCAGAG AATGAACGCA TTGCCAGAGC TTTCAACAAT GACAGAGCTC 
ACGAATCCTC GATTCTGCAC TGCTAATGAA GAAACCAGAA GTCTTGCATA CAATGTGAAG 
GATGAAACCT TGCGATTGGT GCTTCATAAT GCGTTTCGTC ATTCCCAAAG CAAAGCCATT 
GAAGACAAGT CATTAAGTGG CTCTTCTGCT AGACGGAAGT TACAAATCAT TGCTTCGGAT 
ACTGAAGTAT CAGCACAGCG GATTGGAACT GAAAACTTGA GATCTACTTC GGTGTCAAAA 
TCAGGTGATT ACGGTTATCT TGTTTTGGAA TCCGAACCAC AAAGAGTCAA ATTCAAAGTT 
AATTCTCCAA TCACCCTTAT GCAAACGATC TTCAGTTATA TTTTCAACCC GACCGCTGTT 
GTCAGTTTTC TTGCTGGACT AATTGGTGTT ACTGTAGCGG TGGTATACAA TAAAATCGCA 
AAAAGTTCTC CTCGAATGAT TGAACACCTA TCAAGCACCG AAAGTGCAGA AACTGAAAGT 
GCATCCCACA GAACTCGAAC TACGTCGTTT GCACCGACAG ATGATGAAAT TGAACGATTT 
GTTGAAGAAG GATCAGATTT GAGTACCACA CCAATTGGAG CAATACATAG GAAACCGCTA 
ATGCCCATTG AAAAGTCAAA CATTGAGACT CATACTCAAC CTCAAATAAA GCCCGTTCAA 
CGACTGGTAA AAACTGACAT TGACACCGAC GAGGATTCAT TTTCAAATGA TGAGAAAAAG 
CGACTTTTGA GAAATCGGAC GATTTCACGC TCAAGTCTCG AAGGATTCAC AAGTAGATTT 
GCCAACGAAT TTGAGGTCAA GAAGGTCATC GGGCATGGTG GTTTTGGTGT TGTGTTCCGT 
GCTCAAAGTA TTACAGACAT GAACGAGTAC GCTGTCAAGA GAATTGCGGT TGCTGATAAT 
GACAAAGCGA GAAATCGTGT GCTCCGAGAA GCAAGAGCTC TTGCGATGTT TGATCATCCT 
GGAATTATTC GGTACTTCTA TGCTTGGGAG GAAAGGCCGC CGAAGGGATT TCAGGAAAAA 
GAAGATGAGA ATCTTCTTGG GAAAATAAAA GCTGAAAAAC TGGCAAAACT TCATGAAATC 
AAAAAAGCAA AGAAGCACAC AAGTGAAGGG AAACGTGTCA GATCAGCAGA CACTGCATCT 
TTTGCGGAGT CATTTGCAAT GCCTCCCGTT GTTGGAAATA CAACTGATGC TGAGAATAGT 
TGGAGTACGT CTGCAAAGCC ACAAGAAGTC GGAGCAAAAC GCACAACTTC CGAAAGCAAG 
CTTGGATTGC ATGGAGGATC TGACAGAACT ACTGCCGAGC TGAAAGAAGA GAGCGTTGCC 
TTCTCAGAAA GTGATGAAGA ATCGGACACT ACAGAGGATA GTAGCTCTAG TGATGAATCA 
CCTTCCTCAT CTTCCGGCTC TTCTATTGAT GATGAGCCCA AGAAGTATAA CTCTTCATCA 
GGAGGAATTG AGTTTGTCGA TGGCTCAGAT GATGTTGATA ATGAAGCTGT GAAAGAAACA 
AAGAAGGAGA TTGCTGTGAT TGAAGAACTC TCAATCCATA ACAGAGCGAT GATCGTTGAG 
ACGGAAAATC AAGAACTGGA GGTTCGAGAA AGAAATGATA CTGGTGACTG TGCCTATTTG 
TATATTGTAA TGCAATTATG TGCCGAGAAG ACCCTTGAAG ATTGGATCCG CCGCAGCAAA 
ACCATGGAAA GTCGTCCGCT ATTCACGATG AAAAATTGGA TTAAACAATT GGCGAGCGGT 
CTTGAATACC TTCACAACAA AGGATTCATC CACAGAGATT TGAAACCAGG AAATGTATTT 
TTCTCTCTCG ACTCAACACA TGGTCATCAA ATCCTCAAAA TTGGAGATCT TGGATTGGCA 
ACTAAAACTG ATGGAGCTCC CAAAATAACA GTTCGCCAGG ATTCAGACAG CAGTGCAAAG 
CATACGAAAA ACGTCGGCAC TCGATCCTAC ATGTCTCCAG AGCAATTGAA ACATCAACAA 
TACACGGAGA AAGTGGATAT TTTCGCACTG GGACTAGTCG CAACAGAGCT CATCATTTCA 
TTTTCTACTG CTAGCGAGAG AATTCACACA TTTGCTGATT TCCAGAAAGG AGATATTCCA 
GCCATTTTGG ACAATGTGCC AGAGTCGCGG GATTTCCTTC TCCAACTGAC CAGCTTGGAA 
CCATCAGAAC GTCCTACTGC CCATGAAGTA GCTACTCATA AATTTCTCCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_509912.1
CDS	77..3310
Translation	MSVYYIVLAGFLLFMALVPFNAGQQYIDDDIEVVSSCQNGYVQNAGCDQNSQVNIIITATLDGVVTALDGETGEMIWRYEDAPLLRGTLSTSDPIDIGGTSLQLMPTLDGRLFSYTHNTNLIEPLPITTDSLLESTIRLGQDAVAGGKSVTTKGFDLFTGEQKYECSMESCGPGDTETPKNPIILIRRTTNSIRAMDTLRGIERWNLSTAEIGVTLAGGITSPTLVSDVKILLQPPDGVIVAVDKYNREEWKTNVDGHIVSVWQVYGNQIGEISIFDPSNIFTTQYEVMQREQHNLQTQSSLLYMGTSNGFPFIIQSPKAKNNLKQRMNALPELSTMTELTNPRFCTANEETRSLAYNVKDETLRLVLHNAFRHSQSKAIEDKSLSGSSARRKLQIIASDTEVSAQRIGTENLRSTSVSKSGDYGYLVLESEPQRVKFKVNSPITLMQTIFSYIFNPTAVVSFLAGLIGVTVAVVYNKIAKSSPRMIEHLSSTESAETESASHRTRTTSFAPTDDEIERFVEEGSDLSTTPIGAIHRKPLMPIEKSNIETHTQPQIKPVQRLVKTDIDTDEDSFSNDEKKRLLRNRTISRSSLEGFTSRFANEFEVKKVIGHGGFGVVFRAQSITDMNEYAVKRIAVADNDKARNRVLREARALAMFDHPGIIRYFYAWEERPPKGFQEKEDENLLGKIKAEKLAKLHEIKKAKKHTSEGKRVRSADTASFAESFAMPPVVGNTTDAENSWSTSAKPQEVGAKRTTSESKLGLHGGSDRTTAELKEESVAFSESDEESDTTEDSSSSDESPSSSSGSSIDDEPKKYNSSSGGIEFVDGSDDVDNEAVKETKKEIAVIEELSIHNRAMIVETENQELEVRERNDTGDCAYLYIVMQLCAEKTLEDWIRRSKTMESRPLFTMKNWIKQLASGLEYLHNKGFIHRDLKPGNVFFSLDSTHGHQILKIGDLGLATKTDGAPKITVRQDSDSSAKHTKNVGTRSYMSPEQLKHQQYTEKVDIFALGLVATELIISFSTASERIHTFADFQKGDIPAILDNVPESRDFLLQLTSLEPSERPTAHEVATHKFLQ

Target ORF information:

RefSeq Version	NM_077511.7
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Eukaryotic translation initiation factor 2-alpha kinase pek-1 (pek-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_077511.7

ORF Insert Sequence:

ATGAGTGTGT ATTATATAGT TTTAGCTGGG TTCTTGCTCT TCATGGCCTT GGTACCATTC 
AACGCTGGCC AACAGTACAT CGATGATGAC ATAGAAGTTG TATCATCTTG TCAAAATGGT 
TATGTACAAA ATGCGGGTTG TGATCAGAAT AGTCAAGTGA ACATTATTAT CACCGCTACT 
CTGGATGGAG TCGTCACTGC ACTGGATGGA GAAACGGGTG AAATGATTTG GAGATACGAG 
GATGCACCAC TTTTAAGAGG AACGCTGAGC ACATCTGACC CGATTGACAT TGGAGGAACC 
AGTTTACAAC TTATGCCAAC ATTAGATGGA AGATTGTTTT CGTACACACA TAACACAAAT 
CTCATTGAAC CACTCCCAAT CACTACTGAT TCTCTTCTGG AATCTACTAT CCGCCTTGGA 
CAGGACGCCG TAGCTGGTGG AAAAAGTGTT ACTACCAAAG GTTTTGACTT ATTCACAGGA 
GAGCAAAAAT ATGAGTGTTC AATGGAGTCT TGTGGACCCG GAGATACGGA AACTCCAAAA 
AATCCAATAA TTTTAATAAG GCGGACTACG AACTCGATTC GTGCAATGGA CACTCTTCGT 
GGAATTGAAA GATGGAACTT ATCTACAGCT GAGATTGGTG TCACTTTGGC TGGCGGAATA 
ACTAGTCCAA CATTAGTTTC TGACGTCAAA ATATTGCTCC AACCCCCAGA CGGAGTGATT 
GTTGCTGTTG ACAAGTACAA CCGCGAGGAA TGGAAAACGA ACGTAGATGG GCACATTGTT 
AGTGTTTGGC AAGTTTATGG AAATCAAATC GGAGAGATAT CCATCTTCGA CCCAAGTAAT 
ATTTTCACCA CTCAATATGA AGTGATGCAG CGTGAACAAC ACAACTTGCA AACTCAGTCA 
TCTCTCCTTT ACATGGGTAC ATCAAATGGA TTTCCATTCA TCATTCAAAG TCCCAAAGCT 
AAGAATAACT TGAAGCAGAG AATGAACGCA TTGCCAGAGC TTTCAACAAT GACAGAGCTC 
ACGAATCCTC GATTCTGCAC TGCTAATGAA GAAACCAGAA GTCTTGCATA CAATGTGAAG 
GATGAAACCT TGCGATTGGT GCTTCATAAT GCGTTTCGTC ATTCCCAAAG CAAAGCCATT 
GAAGACAAGT CATTAAGTGG CTCTTCTGCT AGACGGAAGT TACAAATCAT TGCTTCGGAT 
ACTGAAGTAT CAGCACAGCG GATTGGAACT GAAAACTTGA GATCTACTTC GGTGTCAAAA 
TCAGGTGATT ACGGTTATCT TGTTTTGGAA TCCGAACCAC AAAGAGTCAA ATTCAAAGTT 
AATTCTCCAA TCACCCTTAT GCAAACGATC TTCAGTTATA TTTTCAACCC GACCGCTGTT 
GTCAGTTTTC TTGCTGGACT AATTGGTGTT ACTGTAGCGG TGGTATACAA TAAAATCGCA 
AAAAGTTCTC CTCGAATGAT TGAACACCTA TCAAGCACCG AAAGTGCAGA AACTGAAAGT 
GCATCCCACA GAACTCGAAC TACGTCGTTT GCACCGACAG ATGATGAAAT TGAACGATTT 
GTTGAAGAAG GATCAGATTT GAGTACCACA CCAATTGGAG CAATACATAG GAAACCGCTA 
ATGCCCATTG AAAAGTCAAA CATTGAGACT CATACTCAAC CTCAAATAAA GCCCGTTCAA 
CGACTGGTAA AAACTGACAT TGACACCGAC GAGGATTCAT TTTCAAATGA TGAGAAAAAG 
CGACTTTTGA GAAATCGGAC GATTTCACGC TCAAGTCTCG AAGGATTCAC AAGTAGATTT 
GCCAACGAAT TTGAGGTCAA GAAGGTCATC GGGCATGGTG GTTTTGGTGT TGTGTTCCGT 
GCTCAAAGTA TTACAGACAT GAACGAGTAC GCTGTCAAGA GAATTGCGGT TGCTGATAAT 
GACAAAGCGA GAAATCGTGT GCTCCGAGAA GCAAGAGCTC TTGCGATGTT TGATCATCCT 
GGAATTATTC GGTACTTCTA TGCTTGGGAG GAAAGGCCGC CGAAGGGATT TCAGGAAAAA 
GAAGATGAGA ATCTTCTTGG GAAAATAAAA GCTGAAAAAC TGGCAAAACT TCATGAAATC 
AAAAAAGCAA AGAAGCACAC AAGTGAAGGG AAACGTGTCA GATCAGCAGA CACTGCATCT 
TTTGCGGAGT CATTTGCAAT GCCTCCCGTT GTTGGAAATA CAACTGATGC TGAGAATAGT 
TGGAGTACGT CTGCAAAGCC ACAAGAAGTC GGAGCAAAAC GCACAACTTC CGAAAGCAAG 
CTTGGATTGC ATGGAGGATC TGACAGAACT ACTGCCGAGC TGAAAGAAGA GAGCGTTGCC 
TTCTCAGAAA GTGATGAAGA ATCGGACACT ACAGAGGATA GTAGCTCTAG TGATGAATCA 
CCTTCCTCAT CTTCCGGCTC TTCTATTGAT GATGAGCCCA AGAAGTATAA CTCTTCATCA 
GGAGGAATTG AGTTTGTCGA TGGCTCAGAT GATGTTGATA ATGAAGCTGT GAAAGAAACA 
AAGAAGGAGA TTGCTGTGAT TGAAGAACTC TCAATCCATA ACAGAGCGAT GATCGTTGAG 
ACGGAAAATC AAGAACTGGA GGTTCGAGAA AGAAATGATA CTGGTGACTG TGCCTATTTG 
TATATTGTAA TGCAATTATG TGCCGAGAAG ACCCTTGAAG ATTGGATCCG CCGCAGCAAA 
ACCATGGAAA GTCGTCCGCT ATTCACGATG AAAAATTGGA TTAAACAATT GGCGAGCGGT 
CTTGAATACC TTCACAACAA AGGATTCATC CACAGAGATT TGAAACCAGG AAATGTATTT 
TTCTCTCTCG ACTCAACACA TGGTCATCAA ATCCTCAAAA TTGGAGATCT TGGATTGGCA 
ACTAAAACTG ATGGAGCTCC CAAAATAACA GTTCGCCAGG ATTCAGACAG CAGTGCAAAG 
CATACGAAAA ACGTCGGCAC TCGATCCTAC ATGTCTCCAG AGCAATTGAA ACATCAACAA 
TACACGGAGA AAGTGGATAT TTTCGCACTG GGACTAGTCG CAACAGAGCT CATCATTTCA 
TTTTCTACTG CTAGCGAGAG AATTCACACA TTTGCTGATT TCCAGAAAGG AGATATTCCA 
GCCATTTTGG ACAATGTGCC AGAGTCGCGG GATTTCCTTC TCCAACTGAC CAGCTTGGAA 
CCATCAGAAC GTCCTACTGC CCATGAAGTA GCTACTCATA AATTTCTCCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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pek-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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