slo-1 gene cDNA ORF clone, Caenorhabditis elegans

mRNA	Protein	Name
NM_001269875.1	NP_001256804.1	Calcium-activated potassium channel slo-1
NM_001029089.2	NP_001024260.1	Calcium-activated potassium channel slo-1
NM_001269876.2	NP_001256805.1	Ion_trans domain-containing protein
NM_001269877.2	NP_001256806.1	Ion_trans domain-containing protein
NM_001029090.2	NP_001024261.1	Calcium-activated potassium channel slo-1
NM_001269876.1	NP_001256805.1	Calcium-activated potassium channel slo-1
NM_001269880.1	NP_001256809.1	Calcium-activated potassium channel slo-1
NM_001269879.1	NP_001256808.1	Calcium-activated potassium channel slo-1
NM_001029090.3	NP_001024261.1	Calcium-activated potassium channel slo-1
NM_001269874.2	NP_001256803.1	Ion_trans domain-containing protein
NM_001269879.2	NP_001256808.1	Ion_trans domain-containing protein
NM_001269877.1	NP_001256806.1	Calcium-activated potassium channel slo-1
NM_001269872.1	NP_001256801.1	Calcium-activated potassium channel slo-1
NM_001029089.1	NP_001024260.1	Calcium-activated potassium channel slo-1
NM_001269878.1	NP_001256807.1	Calcium-activated potassium channel slo-1
NM_001269875.2	NP_001256804.1	Ion_trans domain-containing protein
NM_001269880.2	NP_001256809.1	Ion_trans domain-containing protein
NM_001029088.1	NP_001024259.1	Calcium-activated potassium channel slo-1
NM_001269881.1	NP_001256810.1	Calcium-activated potassium channel slo-1
NM_001029088.2	NP_001024259.1	Calcium-activated potassium channel slo-1
NM_001269872.2	NP_001256801.1	Ion_trans domain-containing protein
NM_001269873.1	NP_001256802.1	Calcium-activated potassium channel slo-1
NM_001269874.1	NP_001256803.1	Calcium-activated potassium channel slo-1
NM_001269873.2	NP_001256802.1	Ion_trans domain-containing protein
NM_001269878.2	NP_001256807.1	Ion_trans domain-containing protein
NM_001269881.2	NP_001256810.1	Ion_trans domain-containing protein

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa345500	NM_001029088.2 Latest version!	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa346262	NM_001029089.2 Latest version!	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa346263	NM_001029090.3 Latest version!	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa346282	NM_001269872.2 Latest version!	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa346283	NM_001269873.2 Latest version!	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa346284	NM_001269879.2 Latest version!	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa346285	NM_001269877.2 Latest version!	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa346286	NM_001269875.2 Latest version!	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa346287	NM_001269881.2 Latest version!	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa346288	NM_001269878.2 Latest version!	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa346289	NM_001269880.2 Latest version!	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa346290	NM_001269874.2 Latest version!	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa346291	NM_001269876.2 Latest version!	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa346291	NM_001269876.1	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa346263	NM_001029090.2	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa346284	NM_001269879.1	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa346285	NM_001269877.1	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa346262	NM_001029089.1	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa346286	NM_001269875.1	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa346289	NM_001269880.1	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa345500	NM_001029088.1	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa346282	NM_001269872.1	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa346290	NM_001269874.1	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa346283	NM_001269873.1	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa346288	NM_001269878.1	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa346287	NM_001269881.1	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

slo-1 ( NM_001029088.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024259.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Price & Availability↑

CloneID	OCa345500
Clone ID Related Accession (Same CDS sequence)	NM_001029088.1 , NM_001029088.2
Accession Version	NM_001029088.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024259.1
CDS	1..3423
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001029088.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029088.1

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001029088.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024259.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), mRNA.

Price & Availability↑

CloneID	OCa345500
Clone ID Related Accession (Same CDS sequence)	NM_001029088.1 , NM_001029088.2
Accession Version	NM_001029088.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001029088.1.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024259.1
CDS	40..3462
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001029088.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029088.2

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001029089.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024260.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), mRNA.

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CloneID	OCa346262
Clone ID Related Accession (Same CDS sequence)	NM_001029089.2 , NM_001029089.1
Accession Version	NM_001029089.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001029089.1.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024260.1
CDS	40..3396
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001029089.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029089.2

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001029089.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024260.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Price & Availability↑

CloneID	OCa346262
Clone ID Related Accession (Same CDS sequence)	NM_001029089.2 , NM_001029089.1
Accession Version	NM_001029089.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024260.1
CDS	1..3357
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001029089.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029089.1

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001029090.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024261.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Price & Availability↑

CloneID	OCa346263
Clone ID Related Accession (Same CDS sequence)	NM_001029090.2 , NM_001029090.3
Accession Version	NM_001029090.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_001029090.1. COMPLETENESS: incomplete on both ends.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGGCC ATCTTCCGGA GGACGCCGAA ATTCGATGAG TATTCCGCCA 
GACGGCAGGG GTGTCGATTT TAGCAAGGAT TTTGAGCAGC AATTCCAAGA CATGAAATAT 
GACAGTACTG GAATGTTCCA CTGGTGTCCA TCCCGTAACC TAGAGGACTG TGTTCTGGAG 
CGCCACCAGG CCGCTATGAC CGTACTCAAC GGCCACGTGG TGGTTTGTTT GTTCGCTGAT 
CAGGATTCTC CGCTAATTGG GCTCCGGAAC TTCATAATGC CACTAAGAAG CTCAAACTTC 
CATTATCATG AGCTCAAACA CGTGGTTATT GTGGGAGACC TGGAATATTT GAGGAAAGAG 
TGGAAGACGC TGTACAACTT GCCGAAAATC TCGATTTTGA ATGGCTCCCC GCTATCTCGA 
GCAGATTTAC GCGCCGTCAA CATTAACCTC TGCGACATGT GTGTCATAAT CTCTGCCCGG 
GTCCCAAATA CCGAAGATAC CACTTTGGCA GATAAGGAAG CGATTCTGGC ATCATTGAAC 
ATTAAAGCTA TGCAATTTGA TGATACCCTT GGCTTTTTCC CGATGAGGCA TCAAACTGGC 
GATCGGAGCC CGCTGGGCTC CCCGATTTCC ATGCAGAAGA AGGGCGCCAA GTTTGGGACC 
AATGTGCCGA TGATTACGGA GCTTGTTAAC GACTCAAATG TGCAATTCCT CGACCAGGAC 
GACGACGACG ATCCGGACAC CGAGCTCTAC CTCACGCAGC CCTTCGCCTG CGGAACTGCC 
TTCGCCATTT CAGTGTTGGA TTCTTTGATG AGTACTACCT ACTTCAACGA CTCGGCCCTG 
ACCCTCATCC GAACTCTGGT CACTGGTGGA GCCACACCGG AGCTTGAACT TATTCTGGCA 
GAAGGCGCCG GGCTCCGAGG TGGCTACAGT ACCCCGGAGA CCCTCTCAAA CAGAGATCGG 
TGCCGAATTG CTCAGATTTC GCTGCAGGAT AACCCGTACG ATGGAGTTGT GCACAACACT 
ACCTATGGTG CAATGTTCAC GATAGCTCTG AGGAGGTACG GCCAACTTTG TATCGGGCTG 
TATAGGCTTC ACGACCAGGA TAATCCGGAC TCTATGAAGC GATATGTTAT CACTAATCCT 
CCAGCAGAAT TGAGGATTAA AAATACTGAT TACGTCTACG TACTTGAACA ATTCGATCCG 
GGACTGGAGT ACGAGCCGGG CAAACGACAC TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024261.1
CDS	1..3396
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001029090.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029090.2

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGGCC ATCTTCCGGA GGACGCCGAA ATTCGATGAG TATTCCGCCA 
GACGGCAGGG GTGTCGATTT TAGCAAGGAT TTTGAGCAGC AATTCCAAGA CATGAAATAT 
GACAGTACTG GAATGTTCCA CTGGTGTCCA TCCCGTAACC TAGAGGACTG TGTTCTGGAG 
CGCCACCAGG CCGCTATGAC CGTACTCAAC GGCCACGTGG TGGTTTGTTT GTTCGCTGAT 
CAGGATTCTC CGCTAATTGG GCTCCGGAAC TTCATAATGC CACTAAGAAG CTCAAACTTC 
CATTATCATG AGCTCAAACA CGTGGTTATT GTGGGAGACC TGGAATATTT GAGGAAAGAG 
TGGAAGACGC TGTACAACTT GCCGAAAATC TCGATTTTGA ATGGCTCCCC GCTATCTCGA 
GCAGATTTAC GCGCCGTCAA CATTAACCTC TGCGACATGT GTGTCATAAT CTCTGCCCGG 
GTCCCAAATA CCGAAGATAC CACTTTGGCA GATAAGGAAG CGATTCTGGC ATCATTGAAC 
ATTAAAGCTA TGCAATTTGA TGATACCCTT GGCTTTTTCC CGATGAGGCA TCAAACTGGC 
GATCGGAGCC CGCTGGGCTC CCCGATTTCC ATGCAGAAGA AGGGCGCCAA GTTTGGGACC 
AATGTGCCGA TGATTACGGA GCTTGTTAAC GACTCAAATG TGCAATTCCT CGACCAGGAC 
GACGACGACG ATCCGGACAC CGAGCTCTAC CTCACGCAGC CCTTCGCCTG CGGAACTGCC 
TTCGCCATTT CAGTGTTGGA TTCTTTGATG AGTACTACCT ACTTCAACGA CTCGGCCCTG 
ACCCTCATCC GAACTCTGGT CACTGGTGGA GCCACACCGG AGCTTGAACT TATTCTGGCA 
GAAGGCGCCG GGCTCCGAGG TGGCTACAGT ACCCCGGAGA CCCTCTCAAA CAGAGATCGG 
TGCCGAATTG CTCAGATTTC GCTGCAGGAT AACCCGTACG ATGGAGTTGT GCACAACACT 
ACCTATGGTG CAATGTTCAC GATAGCTCTG AGGAGGTACG GCCAACTTTG TATCGGGCTG 
TATAGGCTTC ACGACCAGGA TAATCCGGAC TCTATGAAGC GATATGTTAT CACTAATCCT 
CCAGCAGAAT TGAGGATTAA AAATACTGAT TACGTCTACG TACTTGAACA ATTCGATCCG 
GGACTGGAGT ACGAGCCGGG CAAACGACAC TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001029090.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024261.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), mRNA.

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CloneID	OCa346263
Clone ID Related Accession (Same CDS sequence)	NM_001029090.2 , NM_001029090.3
Accession Version	NM_001029090.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001029090.2.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGGCC ATCTTCCGGA GGACGCCGAA ATTCGATGAG TATTCCGCCA 
GACGGCAGGG GTGTCGATTT TAGCAAGGAT TTTGAGCAGC AATTCCAAGA CATGAAATAT 
GACAGTACTG GAATGTTCCA CTGGTGTCCA TCCCGTAACC TAGAGGACTG TGTTCTGGAG 
CGCCACCAGG CCGCTATGAC CGTACTCAAC GGCCACGTGG TGGTTTGTTT GTTCGCTGAT 
CAGGATTCTC CGCTAATTGG GCTCCGGAAC TTCATAATGC CACTAAGAAG CTCAAACTTC 
CATTATCATG AGCTCAAACA CGTGGTTATT GTGGGAGACC TGGAATATTT GAGGAAAGAG 
TGGAAGACGC TGTACAACTT GCCGAAAATC TCGATTTTGA ATGGCTCCCC GCTATCTCGA 
GCAGATTTAC GCGCCGTCAA CATTAACCTC TGCGACATGT GTGTCATAAT CTCTGCCCGG 
GTCCCAAATA CCGAAGATAC CACTTTGGCA GATAAGGAAG CGATTCTGGC ATCATTGAAC 
ATTAAAGCTA TGCAATTTGA TGATACCCTT GGCTTTTTCC CGATGAGGCA TCAAACTGGC 
GATCGGAGCC CGCTGGGCTC CCCGATTTCC ATGCAGAAGA AGGGCGCCAA GTTTGGGACC 
AATGTGCCGA TGATTACGGA GCTTGTTAAC GACTCAAATG TGCAATTCCT CGACCAGGAC 
GACGACGACG ATCCGGACAC CGAGCTCTAC CTCACGCAGC CCTTCGCCTG CGGAACTGCC 
TTCGCCATTT CAGTGTTGGA TTCTTTGATG AGTACTACCT ACTTCAACGA CTCGGCCCTG 
ACCCTCATCC GAACTCTGGT CACTGGTGGA GCCACACCGG AGCTTGAACT TATTCTGGCA 
GAAGGCGCCG GGCTCCGAGG TGGCTACAGT ACCCCGGAGA CCCTCTCAAA CAGAGATCGG 
TGCCGAATTG CTCAGATTTC GCTGCAGGAT AACCCGTACG ATGGAGTTGT GCACAACACT 
ACCTATGGTG CAATGTTCAC GATAGCTCTG AGGAGGTACG GCCAACTTTG TATCGGGCTG 
TATAGGCTTC ACGACCAGGA TAATCCGGAC TCTATGAAGC GATATGTTAT CACTAATCCT 
CCAGCAGAAT TGAGGATTAA AAATACTGAT TACGTCTACG TACTTGAACA ATTCGATCCG 
GGACTGGAGT ACGAGCCGGG CAAACGACAC TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024261.1
CDS	40..3435
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001029090.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029090.3

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGGCC ATCTTCCGGA GGACGCCGAA ATTCGATGAG TATTCCGCCA 
GACGGCAGGG GTGTCGATTT TAGCAAGGAT TTTGAGCAGC AATTCCAAGA CATGAAATAT 
GACAGTACTG GAATGTTCCA CTGGTGTCCA TCCCGTAACC TAGAGGACTG TGTTCTGGAG 
CGCCACCAGG CCGCTATGAC CGTACTCAAC GGCCACGTGG TGGTTTGTTT GTTCGCTGAT 
CAGGATTCTC CGCTAATTGG GCTCCGGAAC TTCATAATGC CACTAAGAAG CTCAAACTTC 
CATTATCATG AGCTCAAACA CGTGGTTATT GTGGGAGACC TGGAATATTT GAGGAAAGAG 
TGGAAGACGC TGTACAACTT GCCGAAAATC TCGATTTTGA ATGGCTCCCC GCTATCTCGA 
GCAGATTTAC GCGCCGTCAA CATTAACCTC TGCGACATGT GTGTCATAAT CTCTGCCCGG 
GTCCCAAATA CCGAAGATAC CACTTTGGCA GATAAGGAAG CGATTCTGGC ATCATTGAAC 
ATTAAAGCTA TGCAATTTGA TGATACCCTT GGCTTTTTCC CGATGAGGCA TCAAACTGGC 
GATCGGAGCC CGCTGGGCTC CCCGATTTCC ATGCAGAAGA AGGGCGCCAA GTTTGGGACC 
AATGTGCCGA TGATTACGGA GCTTGTTAAC GACTCAAATG TGCAATTCCT CGACCAGGAC 
GACGACGACG ATCCGGACAC CGAGCTCTAC CTCACGCAGC CCTTCGCCTG CGGAACTGCC 
TTCGCCATTT CAGTGTTGGA TTCTTTGATG AGTACTACCT ACTTCAACGA CTCGGCCCTG 
ACCCTCATCC GAACTCTGGT CACTGGTGGA GCCACACCGG AGCTTGAACT TATTCTGGCA 
GAAGGCGCCG GGCTCCGAGG TGGCTACAGT ACCCCGGAGA CCCTCTCAAA CAGAGATCGG 
TGCCGAATTG CTCAGATTTC GCTGCAGGAT AACCCGTACG ATGGAGTTGT GCACAACACT 
ACCTATGGTG CAATGTTCAC GATAGCTCTG AGGAGGTACG GCCAACTTTG TATCGGGCTG 
TATAGGCTTC ACGACCAGGA TAATCCGGAC TCTATGAAGC GATATGTTAT CACTAATCCT 
CCAGCAGAAT TGAGGATTAA AAATACTGAT TACGTCTACG TACTTGAACA ATTCGATCCG 
GGACTGGAGT ACGAGCCGGG CAAACGACAC TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269872.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256801.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

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CloneID	OCa346282
Clone ID Related Accession (Same CDS sequence)	NM_001269872.1 , NM_001269872.2
Accession Version	NM_001269872.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGATT CTCTAGCGAT GCAGCTTTAC AGAAGTATTC GGGACCGCAG GGCCAAGGAC 
TGGGCTGGCG AGCTGATATC CGGACAAAGT CTGACGGGAC GGTTTCTGGT ACTTTTGGTG 
TTCATTTTAT CGATTGGATC GTTGATTATT TATTTTTACG ACGCTAGTTT CCAAAACTTC 
CAAGTAGAAA CGTGCATCCC GTGGCAGGAC AGTCCCTCGC AACAAATCGA CCTGGGCTTC 
AACATATTTT TTCTCGTCTA TTTTTTCATT AGATTCATCG CCGCGTCCGA CAAAGTTTGG 
TTTCTGCTTG AAATGTACAG CTGGATTGAT TTTTTCACGA TTCCGCCAAG CTTCGTGGCA 
ATTTACTTGC AGAGGAATTG GCTCGGATTC CGTTTCCTCC GTGCTCTTCG CCTCATGACC 
GTACCCGACA TTCTACAGTA CCTCAACATC CTGAAAACAT CTTCATCAAT CCGATTGACA 
CAGTTGGTCA CAATTTTCGT GGCGGTTTGT CTGACTGGAG CCGGCTTGGT GCACTTACTG 
GAAAATTCTG GGGACTTCTT CAAAGGATTT ATAAATCCGC ACAGAATCAC TTATGCGGAC 
TCTGTGTACT TTGTACTGGT CACAATGTCT ACTGTCGGAT ATGGAGATAT CTATTGTACG 
ACGCTTTGCG GCCGACTTTT CATGATTTTC TTCATTCTGT TCGGTTTGGC CATGTTTGCA 
AGTTACGTAC CAGAAATTGC CGATTTGATT GGAAACCGGC AAAAATACGG TGGGGAGTAC 
AAAGGAGAGC ACGGGAAGAA GCACATAGTG GTCTGTGGCC ATATCACCTA CGATTCGGTG 
TCCCATTTTC TTCAAGATTT CCTACACGAG GACCGTGATG ACGTGGATGT CGAAGTGGTG 
TTTTTGCATC GTGTCGTGCC GGATTTGGAG CTGGAAGGCT TGTTTAAGCG GCATTTCACA 
AAAGTCGAAT TTTTCACGGG GACTGTCATG GATTCTCTGG ATCTTAGCAG AGTCAAGATC 
GGGGACGCCG ACGCCTGCCT GGTCCTTGCC AACAAGTACA GTACCAATCC GGACGCCGAG 
GACGCCGCAA ACATTATGCG TGTAATTTCG ATCAAAAACT ACTCATCGGA CATCAGAGTT 
ATTGTGCAAC TAATGCAATA TCACAATAAG GCCTACCTTC TAAATATCCC CTCCTGGGAC 
TGGAAACGAG GTGATGACGT CATCTGTCTC GCGGAGCTTA AGCTCGGATT CATTGCTCAA 
AGCTGTCTGG CACCCGGATT CTCTACGATG ATGGCTAATT TATTTGCAAT GAGAAGTTTC 
AAAACGTCAC AAACCACACC GGACTGGCTG AACCTGTACC TTTGCGGTGC CGGAATGGAA 
ATGTACACCG ACACGCTATC CCACTCGTTC GTCGGAATGA CTTTTCCTGA AGCTGTCGAC 
CTATTATTCA ACCGCCTGGG GCTTCTTCTT CTGGCGATTG AGCTTAAAGA TGAGGAAAAT 
AAAGAATGCA ATATTGCAAT CAATCCAGGC CCACATATTG TCATCCAGCC TCAAACTCAA 
GGATTTTTCA TTGCTCAGAG CGCTGACGAG GTGAAGCGGG CGTTTTTCTG GTGTAAGCAG 
TGTCATGACG ATATTAAGGA CGTTTCCTTG ATCAAAAAAT GTAAATGCAA AAATTTGGCC 
CTCTTCCGTC GCAACACCAA ACACTCGACA GCGGCCCGTG ATTATTCGGA CTTTGACGCT 
CTGTTTTATC AGAATGACGC AAGAGCCACC GATGTGCTCC AGCAGTTCCA GCCACAAGCG 
CCAGCAGGTC CGATGGGCCA TTTGGGCCAA CAAGTTCAGC TAAGGATGAT TAATCAACAG 
AGGCCATCTT CCGGAGGACG CCGAAATTCG ATGAGTATTC CGCCAGACGG CAGGGGTGTC 
GATTTTAGCA AGGATTTTGA GCAGCAATTC CAAGACATGA AATATGACAG TACTGGAATG 
TTCCACTGGT GTCCATCCCG TAACCTAGAG GACTGTGTTC TGGAGCGCCA CCAGGCCGCT 
ATGACCGTAC TCAACGGCCA CGTGGTGGTT TGTTTGTTCG CTGATCAGGA TTCTCCGCTA 
ATTGGGCTCC GGAACTTCAT AATGCCACTA AGAAGCTCAA ACTTCCATTA TCATGAGCTC 
AAACACGTGG TTATTGTGGG AGACCTGGAA TATTTGAGGA AAGAGTGGAA GACGCTGTAC 
AACTTGCCGA AAATCTCGAT TTTGAATGGC TCCCCGCTAT CTCGAGCAGA TTTACGCGCC 
GTCAACATTA ACCTCTGCGA CATGTGTGTC ATAATCTCTG CCCGGGTCCC AAATACCGAA 
GATACCACTT TGGCAGATAA GGAAGCGATT CTGGCATCAT TGAACATTAA AGCTATGCAA 
TTTGATGATA CCCTTGGCTT TTTCCCGATG AGGCATCAAA CTGGCGATCG GAGCCCGCTG 
GGCTCCCCGA TTTCCATGCA GAAGAAGGGC GCCAAGTTTG GGACCAATGT GCCGATGATT 
ACGGAGCTTG TTAACGACTC AAATGTGCAA TTCCTCGACC AGGACGACGA CGACGATCCG 
GACACCGAGC TCTACCTCAC GCAGCCCTTC GCCTGCGGAA CTGCCTTCGC CATTTCAGTG 
TTGGATTCTT TGATGAGTAC TACCTACTTC AACGACTCGG CCCTGACCCT CATCCGAACT 
CTGGTCACTG GTGGAGCCAC ACCGGAGCTT GAACTTATTC TGGCAGAAGG CGCCGGGCTC 
CGAGGTGGCT ACAGTACCCC GGAGACCCTC TCAAACAGAG ATCGGTGCCG AATTGCTCAG 
ATTTCGCTGC AGGATAACCC GTACGATGGA GTTGTGCACA ACACTACCTA TGGTGCAATG 
TTCACGATAG CTCTGAGGAG GTACGGCCAA CTTTGTATCG GGCTGTATAG GCTTCACGAC 
CAGGATAATC CGGACTCTAT GAAGCGATAT GTTATCACTA ATCCTCCAGC AGAATTGAGG 
ATTAAAAATA CTGATTACGT CTACGTACTT GAACAATTCG ATCCGGGACT GGAGTACGAG 
CCGGGCAAAC GACACTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256801.1
CDS	1..3441
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEDSLAMQLYRSIRDRRAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269872.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269872.1

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGATT CTCTAGCGAT GCAGCTTTAC AGAAGTATTC GGGACCGCAG GGCCAAGGAC 
TGGGCTGGCG AGCTGATATC CGGACAAAGT CTGACGGGAC GGTTTCTGGT ACTTTTGGTG 
TTCATTTTAT CGATTGGATC GTTGATTATT TATTTTTACG ACGCTAGTTT CCAAAACTTC 
CAAGTAGAAA CGTGCATCCC GTGGCAGGAC AGTCCCTCGC AACAAATCGA CCTGGGCTTC 
AACATATTTT TTCTCGTCTA TTTTTTCATT AGATTCATCG CCGCGTCCGA CAAAGTTTGG 
TTTCTGCTTG AAATGTACAG CTGGATTGAT TTTTTCACGA TTCCGCCAAG CTTCGTGGCA 
ATTTACTTGC AGAGGAATTG GCTCGGATTC CGTTTCCTCC GTGCTCTTCG CCTCATGACC 
GTACCCGACA TTCTACAGTA CCTCAACATC CTGAAAACAT CTTCATCAAT CCGATTGACA 
CAGTTGGTCA CAATTTTCGT GGCGGTTTGT CTGACTGGAG CCGGCTTGGT GCACTTACTG 
GAAAATTCTG GGGACTTCTT CAAAGGATTT ATAAATCCGC ACAGAATCAC TTATGCGGAC 
TCTGTGTACT TTGTACTGGT CACAATGTCT ACTGTCGGAT ATGGAGATAT CTATTGTACG 
ACGCTTTGCG GCCGACTTTT CATGATTTTC TTCATTCTGT TCGGTTTGGC CATGTTTGCA 
AGTTACGTAC CAGAAATTGC CGATTTGATT GGAAACCGGC AAAAATACGG TGGGGAGTAC 
AAAGGAGAGC ACGGGAAGAA GCACATAGTG GTCTGTGGCC ATATCACCTA CGATTCGGTG 
TCCCATTTTC TTCAAGATTT CCTACACGAG GACCGTGATG ACGTGGATGT CGAAGTGGTG 
TTTTTGCATC GTGTCGTGCC GGATTTGGAG CTGGAAGGCT TGTTTAAGCG GCATTTCACA 
AAAGTCGAAT TTTTCACGGG GACTGTCATG GATTCTCTGG ATCTTAGCAG AGTCAAGATC 
GGGGACGCCG ACGCCTGCCT GGTCCTTGCC AACAAGTACA GTACCAATCC GGACGCCGAG 
GACGCCGCAA ACATTATGCG TGTAATTTCG ATCAAAAACT ACTCATCGGA CATCAGAGTT 
ATTGTGCAAC TAATGCAATA TCACAATAAG GCCTACCTTC TAAATATCCC CTCCTGGGAC 
TGGAAACGAG GTGATGACGT CATCTGTCTC GCGGAGCTTA AGCTCGGATT CATTGCTCAA 
AGCTGTCTGG CACCCGGATT CTCTACGATG ATGGCTAATT TATTTGCAAT GAGAAGTTTC 
AAAACGTCAC AAACCACACC GGACTGGCTG AACCTGTACC TTTGCGGTGC CGGAATGGAA 
ATGTACACCG ACACGCTATC CCACTCGTTC GTCGGAATGA CTTTTCCTGA AGCTGTCGAC 
CTATTATTCA ACCGCCTGGG GCTTCTTCTT CTGGCGATTG AGCTTAAAGA TGAGGAAAAT 
AAAGAATGCA ATATTGCAAT CAATCCAGGC CCACATATTG TCATCCAGCC TCAAACTCAA 
GGATTTTTCA TTGCTCAGAG CGCTGACGAG GTGAAGCGGG CGTTTTTCTG GTGTAAGCAG 
TGTCATGACG ATATTAAGGA CGTTTCCTTG ATCAAAAAAT GTAAATGCAA AAATTTGGCC 
CTCTTCCGTC GCAACACCAA ACACTCGACA GCGGCCCGTG ATTATTCGGA CTTTGACGCT 
CTGTTTTATC AGAATGACGC AAGAGCCACC GATGTGCTCC AGCAGTTCCA GCCACAAGCG 
CCAGCAGGTC CGATGGGCCA TTTGGGCCAA CAAGTTCAGC TAAGGATGAT TAATCAACAG 
AGGCCATCTT CCGGAGGACG CCGAAATTCG ATGAGTATTC CGCCAGACGG CAGGGGTGTC 
GATTTTAGCA AGGATTTTGA GCAGCAATTC CAAGACATGA AATATGACAG TACTGGAATG 
TTCCACTGGT GTCCATCCCG TAACCTAGAG GACTGTGTTC TGGAGCGCCA CCAGGCCGCT 
ATGACCGTAC TCAACGGCCA CGTGGTGGTT TGTTTGTTCG CTGATCAGGA TTCTCCGCTA 
ATTGGGCTCC GGAACTTCAT AATGCCACTA AGAAGCTCAA ACTTCCATTA TCATGAGCTC 
AAACACGTGG TTATTGTGGG AGACCTGGAA TATTTGAGGA AAGAGTGGAA GACGCTGTAC 
AACTTGCCGA AAATCTCGAT TTTGAATGGC TCCCCGCTAT CTCGAGCAGA TTTACGCGCC 
GTCAACATTA ACCTCTGCGA CATGTGTGTC ATAATCTCTG CCCGGGTCCC AAATACCGAA 
GATACCACTT TGGCAGATAA GGAAGCGATT CTGGCATCAT TGAACATTAA AGCTATGCAA 
TTTGATGATA CCCTTGGCTT TTTCCCGATG AGGCATCAAA CTGGCGATCG GAGCCCGCTG 
GGCTCCCCGA TTTCCATGCA GAAGAAGGGC GCCAAGTTTG GGACCAATGT GCCGATGATT 
ACGGAGCTTG TTAACGACTC AAATGTGCAA TTCCTCGACC AGGACGACGA CGACGATCCG 
GACACCGAGC TCTACCTCAC GCAGCCCTTC GCCTGCGGAA CTGCCTTCGC CATTTCAGTG 
TTGGATTCTT TGATGAGTAC TACCTACTTC AACGACTCGG CCCTGACCCT CATCCGAACT 
CTGGTCACTG GTGGAGCCAC ACCGGAGCTT GAACTTATTC TGGCAGAAGG CGCCGGGCTC 
CGAGGTGGCT ACAGTACCCC GGAGACCCTC TCAAACAGAG ATCGGTGCCG AATTGCTCAG 
ATTTCGCTGC AGGATAACCC GTACGATGGA GTTGTGCACA ACACTACCTA TGGTGCAATG 
TTCACGATAG CTCTGAGGAG GTACGGCCAA CTTTGTATCG GGCTGTATAG GCTTCACGAC 
CAGGATAATC CGGACTCTAT GAAGCGATAT GTTATCACTA ATCCTCCAGC AGAATTGAGG 
ATTAAAAATA CTGATTACGT CTACGTACTT GAACAATTCG ATCCGGGACT GGAGTACGAG 
CCGGGCAAAC GACACTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269872.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256801.1 Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

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CloneID	OCa346282
Clone ID Related Accession (Same CDS sequence)	NM_001269872.1 , NM_001269872.2
Accession Version	NM_001269872.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Ion_trans domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269872.1.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGATT CTCTAGCGAT GCAGCTTTAC AGAAGTATTC GGGACCGCAG GGCCAAGGAC 
TGGGCTGGCG AGCTGATATC CGGACAAAGT CTGACGGGAC GGTTTCTGGT ACTTTTGGTG 
TTCATTTTAT CGATTGGATC GTTGATTATT TATTTTTACG ACGCTAGTTT CCAAAACTTC 
CAAGTAGAAA CGTGCATCCC GTGGCAGGAC AGTCCCTCGC AACAAATCGA CCTGGGCTTC 
AACATATTTT TTCTCGTCTA TTTTTTCATT AGATTCATCG CCGCGTCCGA CAAAGTTTGG 
TTTCTGCTTG AAATGTACAG CTGGATTGAT TTTTTCACGA TTCCGCCAAG CTTCGTGGCA 
ATTTACTTGC AGAGGAATTG GCTCGGATTC CGTTTCCTCC GTGCTCTTCG CCTCATGACC 
GTACCCGACA TTCTACAGTA CCTCAACATC CTGAAAACAT CTTCATCAAT CCGATTGACA 
CAGTTGGTCA CAATTTTCGT GGCGGTTTGT CTGACTGGAG CCGGCTTGGT GCACTTACTG 
GAAAATTCTG GGGACTTCTT CAAAGGATTT ATAAATCCGC ACAGAATCAC TTATGCGGAC 
TCTGTGTACT TTGTACTGGT CACAATGTCT ACTGTCGGAT ATGGAGATAT CTATTGTACG 
ACGCTTTGCG GCCGACTTTT CATGATTTTC TTCATTCTGT TCGGTTTGGC CATGTTTGCA 
AGTTACGTAC CAGAAATTGC CGATTTGATT GGAAACCGGC AAAAATACGG TGGGGAGTAC 
AAAGGAGAGC ACGGGAAGAA GCACATAGTG GTCTGTGGCC ATATCACCTA CGATTCGGTG 
TCCCATTTTC TTCAAGATTT CCTACACGAG GACCGTGATG ACGTGGATGT CGAAGTGGTG 
TTTTTGCATC GTGTCGTGCC GGATTTGGAG CTGGAAGGCT TGTTTAAGCG GCATTTCACA 
AAAGTCGAAT TTTTCACGGG GACTGTCATG GATTCTCTGG ATCTTAGCAG AGTCAAGATC 
GGGGACGCCG ACGCCTGCCT GGTCCTTGCC AACAAGTACA GTACCAATCC GGACGCCGAG 
GACGCCGCAA ACATTATGCG TGTAATTTCG ATCAAAAACT ACTCATCGGA CATCAGAGTT 
ATTGTGCAAC TAATGCAATA TCACAATAAG GCCTACCTTC TAAATATCCC CTCCTGGGAC 
TGGAAACGAG GTGATGACGT CATCTGTCTC GCGGAGCTTA AGCTCGGATT CATTGCTCAA 
AGCTGTCTGG CACCCGGATT CTCTACGATG ATGGCTAATT TATTTGCAAT GAGAAGTTTC 
AAAACGTCAC AAACCACACC GGACTGGCTG AACCTGTACC TTTGCGGTGC CGGAATGGAA 
ATGTACACCG ACACGCTATC CCACTCGTTC GTCGGAATGA CTTTTCCTGA AGCTGTCGAC 
CTATTATTCA ACCGCCTGGG GCTTCTTCTT CTGGCGATTG AGCTTAAAGA TGAGGAAAAT 
AAAGAATGCA ATATTGCAAT CAATCCAGGC CCACATATTG TCATCCAGCC TCAAACTCAA 
GGATTTTTCA TTGCTCAGAG CGCTGACGAG GTGAAGCGGG CGTTTTTCTG GTGTAAGCAG 
TGTCATGACG ATATTAAGGA CGTTTCCTTG ATCAAAAAAT GTAAATGCAA AAATTTGGCC 
CTCTTCCGTC GCAACACCAA ACACTCGACA GCGGCCCGTG ATTATTCGGA CTTTGACGCT 
CTGTTTTATC AGAATGACGC AAGAGCCACC GATGTGCTCC AGCAGTTCCA GCCACAAGCG 
CCAGCAGGTC CGATGGGCCA TTTGGGCCAA CAAGTTCAGC TAAGGATGAT TAATCAACAG 
AGGCCATCTT CCGGAGGACG CCGAAATTCG ATGAGTATTC CGCCAGACGG CAGGGGTGTC 
GATTTTAGCA AGGATTTTGA GCAGCAATTC CAAGACATGA AATATGACAG TACTGGAATG 
TTCCACTGGT GTCCATCCCG TAACCTAGAG GACTGTGTTC TGGAGCGCCA CCAGGCCGCT 
ATGACCGTAC TCAACGGCCA CGTGGTGGTT TGTTTGTTCG CTGATCAGGA TTCTCCGCTA 
ATTGGGCTCC GGAACTTCAT AATGCCACTA AGAAGCTCAA ACTTCCATTA TCATGAGCTC 
AAACACGTGG TTATTGTGGG AGACCTGGAA TATTTGAGGA AAGAGTGGAA GACGCTGTAC 
AACTTGCCGA AAATCTCGAT TTTGAATGGC TCCCCGCTAT CTCGAGCAGA TTTACGCGCC 
GTCAACATTA ACCTCTGCGA CATGTGTGTC ATAATCTCTG CCCGGGTCCC AAATACCGAA 
GATACCACTT TGGCAGATAA GGAAGCGATT CTGGCATCAT TGAACATTAA AGCTATGCAA 
TTTGATGATA CCCTTGGCTT TTTCCCGATG AGGCATCAAA CTGGCGATCG GAGCCCGCTG 
GGCTCCCCGA TTTCCATGCA GAAGAAGGGC GCCAAGTTTG GGACCAATGT GCCGATGATT 
ACGGAGCTTG TTAACGACTC AAATGTGCAA TTCCTCGACC AGGACGACGA CGACGATCCG 
GACACCGAGC TCTACCTCAC GCAGCCCTTC GCCTGCGGAA CTGCCTTCGC CATTTCAGTG 
TTGGATTCTT TGATGAGTAC TACCTACTTC AACGACTCGG CCCTGACCCT CATCCGAACT 
CTGGTCACTG GTGGAGCCAC ACCGGAGCTT GAACTTATTC TGGCAGAAGG CGCCGGGCTC 
CGAGGTGGCT ACAGTACCCC GGAGACCCTC TCAAACAGAG ATCGGTGCCG AATTGCTCAG 
ATTTCGCTGC AGGATAACCC GTACGATGGA GTTGTGCACA ACACTACCTA TGGTGCAATG 
TTCACGATAG CTCTGAGGAG GTACGGCCAA CTTTGTATCG GGCTGTATAG GCTTCACGAC 
CAGGATAATC CGGACTCTAT GAAGCGATAT GTTATCACTA ATCCTCCAGC AGAATTGAGG 
ATTAAAAATA CTGATTACGT CTACGTACTT GAACAATTCG ATCCGGGACT GGAGTACGAG 
CCGGGCAAAC GACACTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256801.1
CDS	42..3482
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEDSLAMQLYRSIRDRRAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269872.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269872.2

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGATT CTCTAGCGAT GCAGCTTTAC AGAAGTATTC GGGACCGCAG GGCCAAGGAC 
TGGGCTGGCG AGCTGATATC CGGACAAAGT CTGACGGGAC GGTTTCTGGT ACTTTTGGTG 
TTCATTTTAT CGATTGGATC GTTGATTATT TATTTTTACG ACGCTAGTTT CCAAAACTTC 
CAAGTAGAAA CGTGCATCCC GTGGCAGGAC AGTCCCTCGC AACAAATCGA CCTGGGCTTC 
AACATATTTT TTCTCGTCTA TTTTTTCATT AGATTCATCG CCGCGTCCGA CAAAGTTTGG 
TTTCTGCTTG AAATGTACAG CTGGATTGAT TTTTTCACGA TTCCGCCAAG CTTCGTGGCA 
ATTTACTTGC AGAGGAATTG GCTCGGATTC CGTTTCCTCC GTGCTCTTCG CCTCATGACC 
GTACCCGACA TTCTACAGTA CCTCAACATC CTGAAAACAT CTTCATCAAT CCGATTGACA 
CAGTTGGTCA CAATTTTCGT GGCGGTTTGT CTGACTGGAG CCGGCTTGGT GCACTTACTG 
GAAAATTCTG GGGACTTCTT CAAAGGATTT ATAAATCCGC ACAGAATCAC TTATGCGGAC 
TCTGTGTACT TTGTACTGGT CACAATGTCT ACTGTCGGAT ATGGAGATAT CTATTGTACG 
ACGCTTTGCG GCCGACTTTT CATGATTTTC TTCATTCTGT TCGGTTTGGC CATGTTTGCA 
AGTTACGTAC CAGAAATTGC CGATTTGATT GGAAACCGGC AAAAATACGG TGGGGAGTAC 
AAAGGAGAGC ACGGGAAGAA GCACATAGTG GTCTGTGGCC ATATCACCTA CGATTCGGTG 
TCCCATTTTC TTCAAGATTT CCTACACGAG GACCGTGATG ACGTGGATGT CGAAGTGGTG 
TTTTTGCATC GTGTCGTGCC GGATTTGGAG CTGGAAGGCT TGTTTAAGCG GCATTTCACA 
AAAGTCGAAT TTTTCACGGG GACTGTCATG GATTCTCTGG ATCTTAGCAG AGTCAAGATC 
GGGGACGCCG ACGCCTGCCT GGTCCTTGCC AACAAGTACA GTACCAATCC GGACGCCGAG 
GACGCCGCAA ACATTATGCG TGTAATTTCG ATCAAAAACT ACTCATCGGA CATCAGAGTT 
ATTGTGCAAC TAATGCAATA TCACAATAAG GCCTACCTTC TAAATATCCC CTCCTGGGAC 
TGGAAACGAG GTGATGACGT CATCTGTCTC GCGGAGCTTA AGCTCGGATT CATTGCTCAA 
AGCTGTCTGG CACCCGGATT CTCTACGATG ATGGCTAATT TATTTGCAAT GAGAAGTTTC 
AAAACGTCAC AAACCACACC GGACTGGCTG AACCTGTACC TTTGCGGTGC CGGAATGGAA 
ATGTACACCG ACACGCTATC CCACTCGTTC GTCGGAATGA CTTTTCCTGA AGCTGTCGAC 
CTATTATTCA ACCGCCTGGG GCTTCTTCTT CTGGCGATTG AGCTTAAAGA TGAGGAAAAT 
AAAGAATGCA ATATTGCAAT CAATCCAGGC CCACATATTG TCATCCAGCC TCAAACTCAA 
GGATTTTTCA TTGCTCAGAG CGCTGACGAG GTGAAGCGGG CGTTTTTCTG GTGTAAGCAG 
TGTCATGACG ATATTAAGGA CGTTTCCTTG ATCAAAAAAT GTAAATGCAA AAATTTGGCC 
CTCTTCCGTC GCAACACCAA ACACTCGACA GCGGCCCGTG ATTATTCGGA CTTTGACGCT 
CTGTTTTATC AGAATGACGC AAGAGCCACC GATGTGCTCC AGCAGTTCCA GCCACAAGCG 
CCAGCAGGTC CGATGGGCCA TTTGGGCCAA CAAGTTCAGC TAAGGATGAT TAATCAACAG 
AGGCCATCTT CCGGAGGACG CCGAAATTCG ATGAGTATTC CGCCAGACGG CAGGGGTGTC 
GATTTTAGCA AGGATTTTGA GCAGCAATTC CAAGACATGA AATATGACAG TACTGGAATG 
TTCCACTGGT GTCCATCCCG TAACCTAGAG GACTGTGTTC TGGAGCGCCA CCAGGCCGCT 
ATGACCGTAC TCAACGGCCA CGTGGTGGTT TGTTTGTTCG CTGATCAGGA TTCTCCGCTA 
ATTGGGCTCC GGAACTTCAT AATGCCACTA AGAAGCTCAA ACTTCCATTA TCATGAGCTC 
AAACACGTGG TTATTGTGGG AGACCTGGAA TATTTGAGGA AAGAGTGGAA GACGCTGTAC 
AACTTGCCGA AAATCTCGAT TTTGAATGGC TCCCCGCTAT CTCGAGCAGA TTTACGCGCC 
GTCAACATTA ACCTCTGCGA CATGTGTGTC ATAATCTCTG CCCGGGTCCC AAATACCGAA 
GATACCACTT TGGCAGATAA GGAAGCGATT CTGGCATCAT TGAACATTAA AGCTATGCAA 
TTTGATGATA CCCTTGGCTT TTTCCCGATG AGGCATCAAA CTGGCGATCG GAGCCCGCTG 
GGCTCCCCGA TTTCCATGCA GAAGAAGGGC GCCAAGTTTG GGACCAATGT GCCGATGATT 
ACGGAGCTTG TTAACGACTC AAATGTGCAA TTCCTCGACC AGGACGACGA CGACGATCCG 
GACACCGAGC TCTACCTCAC GCAGCCCTTC GCCTGCGGAA CTGCCTTCGC CATTTCAGTG 
TTGGATTCTT TGATGAGTAC TACCTACTTC AACGACTCGG CCCTGACCCT CATCCGAACT 
CTGGTCACTG GTGGAGCCAC ACCGGAGCTT GAACTTATTC TGGCAGAAGG CGCCGGGCTC 
CGAGGTGGCT ACAGTACCCC GGAGACCCTC TCAAACAGAG ATCGGTGCCG AATTGCTCAG 
ATTTCGCTGC AGGATAACCC GTACGATGGA GTTGTGCACA ACACTACCTA TGGTGCAATG 
TTCACGATAG CTCTGAGGAG GTACGGCCAA CTTTGTATCG GGCTGTATAG GCTTCACGAC 
CAGGATAATC CGGACTCTAT GAAGCGATAT GTTATCACTA ATCCTCCAGC AGAATTGAGG 
ATTAAAAATA CTGATTACGT CTACGTACTT GAACAATTCG ATCCGGGACT GGAGTACGAG 
CCGGGCAAAC GACACTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269873.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256802.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

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CloneID	OCa346283
Clone ID Related Accession (Same CDS sequence)	NM_001269873.1 , NM_001269873.2
Accession Version	NM_001269873.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256802.1
CDS	1..3483
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAAREKNAKISDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269873.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269873.1

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269873.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256802.1 Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

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CloneID	OCa346283
Clone ID Related Accession (Same CDS sequence)	NM_001269873.1 , NM_001269873.2
Accession Version	NM_001269873.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Ion_trans domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269873.1.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256802.1
CDS	40..3522
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAAREKNAKISDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269873.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269873.2

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269879.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256808.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

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CloneID	OCa346284
Clone ID Related Accession (Same CDS sequence)	NM_001269879.1 , NM_001269879.2
Accession Version	NM_001269879.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256808.1
CDS	1..3417
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAAREKNAKISDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269879.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269879.1

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269879.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256808.1 Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

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CloneID	OCa346284
Clone ID Related Accession (Same CDS sequence)	NM_001269879.1 , NM_001269879.2
Accession Version	NM_001269879.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Ion_trans domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269879.1.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256808.1
CDS	40..3456
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAAREKNAKISDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269879.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269879.2

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269877.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256806.1 Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

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CloneID	OCa346285
Clone ID Related Accession (Same CDS sequence)	NM_001269877.2 , NM_001269877.1
Accession Version	NM_001269877.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Ion_trans domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269877.1.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256806.1
CDS	40..3396
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269877.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269877.2

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269877.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256806.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Price & Availability↑

CloneID	OCa346285
Clone ID Related Accession (Same CDS sequence)	NM_001269877.2 , NM_001269877.1
Accession Version	NM_001269877.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256806.1
CDS	1..3357
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269877.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269877.1

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269875.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256804.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Price & Availability↑

CloneID	OCa346286
Clone ID Related Accession (Same CDS sequence)	NM_001269875.1 , NM_001269875.2
Accession Version	NM_001269875.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGTTC GACGAGTGAT ACTCATCTCA ACACGAAATC CCTTCGATTT 
GCCTATGAAA TCAAAAAACT CATGCCATCT TCCGGAGGAC GCCGAAATTC GATGAGTATT 
CCGCCAGACG GCAGGGGTGT CGATTTTAGC AAGGATTTTG AGCAGCAATT CCAAGACATG 
AAATATGACA GTACTGGAAT GTTCCACTGG TGTCCATCCC GTAACCTAGA GGACTGTGTT 
CTGGAGCGCC ACCAGGCCGC TATGACCGTA CTCAACGGCC ACGTGGTGGT TTGTTTGTTC 
GCTGATCAGG ATTCTCCGCT AATTGGGCTC CGGAACTTCA TAATGCCACT AAGAAGCTCA 
AACTTCCATT ATCATGAGCT CAAACACGTG GTTATTGTGG GAGACCTGGA ATATTTGAGG 
AAAGAGTGGA AGACGCTGTA CAACTTGCCG AAAATCTCGA TTTTGAATGG CTCCCCGCTA 
TCTCGAGCAG ATTTACGCGC CGTCAACATT AACCTCTGCG ACATGTGTGT CATAATCTCT 
GCCCGGGTCC CAAATACCGA AGATACCACT TTGGCAGATA AGGAAGCGAT TCTGGCATCA 
TTGAACATTA AAGCTATGCA ATTTGATGAT ACCCTTGGCT TTTTCCCGAT GAGGCATCAA 
ACTGGCGATC GGAGCCCGCT GGGCTCCCCG ATTTCCATGC AGAAGAAGGG CGCCAAGTTT 
GGGACCAATG TGCCGATGAT TACGGAGCTT GTTAACGACT CAAATGTGCA ATTCCTCGAC 
CAGGACGACG ACGACGATCC GGACACCGAG CTCTACCTCA CGCAGCCCTT CGCCTGCGGA 
ACTGCCTTCG CCATTTCAGT GTTGGATTCT TTGATGAGTA CTACCTACTT CAACGACTCG 
GCCCTGACCC TCATCCGAAC TCTGGTCACT GGTGGAGCCA CACCGGAGCT TGAACTTATT 
CTGGCAGAAG GCGCCGGGCT CCGAGGTGGC TACAGTACCC CGGAGACCCT CTCAAACAGA 
GATCGGTGCC GAATTGCTCA GATTTCGCTG CAGGATAACC CGTACGATGG AGTTGTGCAC 
AACACTACCT ATGGTGCAAT GTTCACGATA GCTCTGAGGA GGTACGGCCA ACTTTGTATC 
GGGCTGTATA GGCTTCACGA CCAGGATAAT CCGGACTCTA TGAAGCGATA TGTTATCACT 
AATCCTCCAG CAGAATTGAG GATTAAAAAT ACTGATTACG TCTACGTACT TGAACAATTC 
GATCCGGGAC TGGAGTACGA GCCGGGCAAA CGACACTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256804.1
CDS	1..3462
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269875.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269875.1

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGTTC GACGAGTGAT ACTCATCTCA ACACGAAATC CCTTCGATTT 
GCCTATGAAA TCAAAAAACT CATGCCATCT TCCGGAGGAC GCCGAAATTC GATGAGTATT 
CCGCCAGACG GCAGGGGTGT CGATTTTAGC AAGGATTTTG AGCAGCAATT CCAAGACATG 
AAATATGACA GTACTGGAAT GTTCCACTGG TGTCCATCCC GTAACCTAGA GGACTGTGTT 
CTGGAGCGCC ACCAGGCCGC TATGACCGTA CTCAACGGCC ACGTGGTGGT TTGTTTGTTC 
GCTGATCAGG ATTCTCCGCT AATTGGGCTC CGGAACTTCA TAATGCCACT AAGAAGCTCA 
AACTTCCATT ATCATGAGCT CAAACACGTG GTTATTGTGG GAGACCTGGA ATATTTGAGG 
AAAGAGTGGA AGACGCTGTA CAACTTGCCG AAAATCTCGA TTTTGAATGG CTCCCCGCTA 
TCTCGAGCAG ATTTACGCGC CGTCAACATT AACCTCTGCG ACATGTGTGT CATAATCTCT 
GCCCGGGTCC CAAATACCGA AGATACCACT TTGGCAGATA AGGAAGCGAT TCTGGCATCA 
TTGAACATTA AAGCTATGCA ATTTGATGAT ACCCTTGGCT TTTTCCCGAT GAGGCATCAA 
ACTGGCGATC GGAGCCCGCT GGGCTCCCCG ATTTCCATGC AGAAGAAGGG CGCCAAGTTT 
GGGACCAATG TGCCGATGAT TACGGAGCTT GTTAACGACT CAAATGTGCA ATTCCTCGAC 
CAGGACGACG ACGACGATCC GGACACCGAG CTCTACCTCA CGCAGCCCTT CGCCTGCGGA 
ACTGCCTTCG CCATTTCAGT GTTGGATTCT TTGATGAGTA CTACCTACTT CAACGACTCG 
GCCCTGACCC TCATCCGAAC TCTGGTCACT GGTGGAGCCA CACCGGAGCT TGAACTTATT 
CTGGCAGAAG GCGCCGGGCT CCGAGGTGGC TACAGTACCC CGGAGACCCT CTCAAACAGA 
GATCGGTGCC GAATTGCTCA GATTTCGCTG CAGGATAACC CGTACGATGG AGTTGTGCAC 
AACACTACCT ATGGTGCAAT GTTCACGATA GCTCTGAGGA GGTACGGCCA ACTTTGTATC 
GGGCTGTATA GGCTTCACGA CCAGGATAAT CCGGACTCTA TGAAGCGATA TGTTATCACT 
AATCCTCCAG CAGAATTGAG GATTAAAAAT ACTGATTACG TCTACGTACT TGAACAATTC 
GATCCGGGAC TGGAGTACGA GCCGGGCAAA CGACACTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269875.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256804.1 Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

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CloneID	OCa346286
Clone ID Related Accession (Same CDS sequence)	NM_001269875.1 , NM_001269875.2
Accession Version	NM_001269875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Ion_trans domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269875.1.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGTTC GACGAGTGAT ACTCATCTCA ACACGAAATC CCTTCGATTT 
GCCTATGAAA TCAAAAAACT CATGCCATCT TCCGGAGGAC GCCGAAATTC GATGAGTATT 
CCGCCAGACG GCAGGGGTGT CGATTTTAGC AAGGATTTTG AGCAGCAATT CCAAGACATG 
AAATATGACA GTACTGGAAT GTTCCACTGG TGTCCATCCC GTAACCTAGA GGACTGTGTT 
CTGGAGCGCC ACCAGGCCGC TATGACCGTA CTCAACGGCC ACGTGGTGGT TTGTTTGTTC 
GCTGATCAGG ATTCTCCGCT AATTGGGCTC CGGAACTTCA TAATGCCACT AAGAAGCTCA 
AACTTCCATT ATCATGAGCT CAAACACGTG GTTATTGTGG GAGACCTGGA ATATTTGAGG 
AAAGAGTGGA AGACGCTGTA CAACTTGCCG AAAATCTCGA TTTTGAATGG CTCCCCGCTA 
TCTCGAGCAG ATTTACGCGC CGTCAACATT AACCTCTGCG ACATGTGTGT CATAATCTCT 
GCCCGGGTCC CAAATACCGA AGATACCACT TTGGCAGATA AGGAAGCGAT TCTGGCATCA 
TTGAACATTA AAGCTATGCA ATTTGATGAT ACCCTTGGCT TTTTCCCGAT GAGGCATCAA 
ACTGGCGATC GGAGCCCGCT GGGCTCCCCG ATTTCCATGC AGAAGAAGGG CGCCAAGTTT 
GGGACCAATG TGCCGATGAT TACGGAGCTT GTTAACGACT CAAATGTGCA ATTCCTCGAC 
CAGGACGACG ACGACGATCC GGACACCGAG CTCTACCTCA CGCAGCCCTT CGCCTGCGGA 
ACTGCCTTCG CCATTTCAGT GTTGGATTCT TTGATGAGTA CTACCTACTT CAACGACTCG 
GCCCTGACCC TCATCCGAAC TCTGGTCACT GGTGGAGCCA CACCGGAGCT TGAACTTATT 
CTGGCAGAAG GCGCCGGGCT CCGAGGTGGC TACAGTACCC CGGAGACCCT CTCAAACAGA 
GATCGGTGCC GAATTGCTCA GATTTCGCTG CAGGATAACC CGTACGATGG AGTTGTGCAC 
AACACTACCT ATGGTGCAAT GTTCACGATA GCTCTGAGGA GGTACGGCCA ACTTTGTATC 
GGGCTGTATA GGCTTCACGA CCAGGATAAT CCGGACTCTA TGAAGCGATA TGTTATCACT 
AATCCTCCAG CAGAATTGAG GATTAAAAAT ACTGATTACG TCTACGTACT TGAACAATTC 
GATCCGGGAC TGGAGTACGA GCCGGGCAAA CGACACTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256804.1
CDS	40..3501
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269875.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269875.2

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGTTC GACGAGTGAT ACTCATCTCA ACACGAAATC CCTTCGATTT 
GCCTATGAAA TCAAAAAACT CATGCCATCT TCCGGAGGAC GCCGAAATTC GATGAGTATT 
CCGCCAGACG GCAGGGGTGT CGATTTTAGC AAGGATTTTG AGCAGCAATT CCAAGACATG 
AAATATGACA GTACTGGAAT GTTCCACTGG TGTCCATCCC GTAACCTAGA GGACTGTGTT 
CTGGAGCGCC ACCAGGCCGC TATGACCGTA CTCAACGGCC ACGTGGTGGT TTGTTTGTTC 
GCTGATCAGG ATTCTCCGCT AATTGGGCTC CGGAACTTCA TAATGCCACT AAGAAGCTCA 
AACTTCCATT ATCATGAGCT CAAACACGTG GTTATTGTGG GAGACCTGGA ATATTTGAGG 
AAAGAGTGGA AGACGCTGTA CAACTTGCCG AAAATCTCGA TTTTGAATGG CTCCCCGCTA 
TCTCGAGCAG ATTTACGCGC CGTCAACATT AACCTCTGCG ACATGTGTGT CATAATCTCT 
GCCCGGGTCC CAAATACCGA AGATACCACT TTGGCAGATA AGGAAGCGAT TCTGGCATCA 
TTGAACATTA AAGCTATGCA ATTTGATGAT ACCCTTGGCT TTTTCCCGAT GAGGCATCAA 
ACTGGCGATC GGAGCCCGCT GGGCTCCCCG ATTTCCATGC AGAAGAAGGG CGCCAAGTTT 
GGGACCAATG TGCCGATGAT TACGGAGCTT GTTAACGACT CAAATGTGCA ATTCCTCGAC 
CAGGACGACG ACGACGATCC GGACACCGAG CTCTACCTCA CGCAGCCCTT CGCCTGCGGA 
ACTGCCTTCG CCATTTCAGT GTTGGATTCT TTGATGAGTA CTACCTACTT CAACGACTCG 
GCCCTGACCC TCATCCGAAC TCTGGTCACT GGTGGAGCCA CACCGGAGCT TGAACTTATT 
CTGGCAGAAG GCGCCGGGCT CCGAGGTGGC TACAGTACCC CGGAGACCCT CTCAAACAGA 
GATCGGTGCC GAATTGCTCA GATTTCGCTG CAGGATAACC CGTACGATGG AGTTGTGCAC 
AACACTACCT ATGGTGCAAT GTTCACGATA GCTCTGAGGA GGTACGGCCA ACTTTGTATC 
GGGCTGTATA GGCTTCACGA CCAGGATAAT CCGGACTCTA TGAAGCGATA TGTTATCACT 
AATCCTCCAG CAGAATTGAG GATTAAAAAT ACTGATTACG TCTACGTACT TGAACAATTC 
GATCCGGGAC TGGAGTACGA GCCGGGCAAA CGACACTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269881.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256810.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

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CloneID	OCa346287
Clone ID Related Accession (Same CDS sequence)	NM_001269881.1 , NM_001269881.2
Accession Version	NM_001269881.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGGCC ATCTTCCGGA GGACGCCGAA ATTCGATGAG TATTCCGCCA 
GACGGCAGGG GTGTCGATTT TAGCAAGGAT TTTGAGCAGC AATTCCAAGA CATGAAATAT 
GACAGTACTG GAATGTTCCA CTGGTGTCCA TCCCGTAACC TAGAGGACTG TGTTCTGGAG 
CGCCACCAGG CCGCTATGAC CGTACTCAAC GGCCACGTGG TGGTTTGTTT GTTCGCTGAT 
CAGGATTCTC CGCTAATTGG GCTCCGGAAC TTCATAATGC CACTAAGAAG CTCAAACTTC 
CATTATCATG AGCTCAAACA CGTGGTTATT GTGGGAGACC TGGAATATTT GAGGAAAGAG 
TGGAAGACGC TGTACAACTT GCCGAAAATC TCGATTTTGA ATGGCTCCCC GCTATCTCGA 
GCAGATTTAC GCGCCGTCAA CATTAACCTC TGCGACATGT GTGTCATAAT CTCTGCCCGG 
GTCCCAAATA CCGAAGATAC CACTTTGGCA GATAAGGAAG CGATTCTGGC ATCATTGAAC 
ATTAAAGCTA TGCAATTTGA TGATACCCTT GGCTTTTTCC CGATGAGGCA TCAAACTGGC 
GATCGGAGCC CGCTGGGCTC CCCGATTTCC ATGCAGAAGA AGGGCGCCAA GTTTGGGACC 
AATGTGCCGA TGATTACGGA GCTTGTTAAC GACTCAAATG TGCAATTCCT CGACCAGGAC 
GACGACGACG ATCCGGACAC CGAGCTCTAC CTCACGCAGC CCTTCGCCTG CGGAACTGCC 
TTCGCCATTT CAGTGTTGGA TTCTTTGATG AGTACTACCT ACTTCAACGA CTCGGCCCTG 
ACCCTCATCC GAACTCTGGT CACTGGTGGA GCCACACCGG AGCTTGAACT TATTCTGGCA 
GAAGGCGCCG GGCTCCGAGG TGGCTACAGT ACCCCGGAGA CCCTCTCAAA CAGAGATCGG 
TGCCGAATTG CTCAGATTTC GCTGCAGGAT AACCCGTACG ATGGAGTTGT GCACAACACT 
ACCTATGGTG CAATGTTCAC GATAGCTCTG AGGAGGTACG GCCAACTTTG TATCGGGCTG 
TATAGGCTTC ACGACCAGGA TAATCCGGAC TCTATGAAGC GATATGTTAT CACTAATCCT 
CCAGCAGAAT TGAGGATTAA AAATACTGAT TACGTCTACG TACTTGAACA ATTCGATCCG 
GGACTGGAGT ACGAGCCGGG CAAACGACAC TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256810.1
CDS	1..3396
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269881.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269881.1

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGGCC ATCTTCCGGA GGACGCCGAA ATTCGATGAG TATTCCGCCA 
GACGGCAGGG GTGTCGATTT TAGCAAGGAT TTTGAGCAGC AATTCCAAGA CATGAAATAT 
GACAGTACTG GAATGTTCCA CTGGTGTCCA TCCCGTAACC TAGAGGACTG TGTTCTGGAG 
CGCCACCAGG CCGCTATGAC CGTACTCAAC GGCCACGTGG TGGTTTGTTT GTTCGCTGAT 
CAGGATTCTC CGCTAATTGG GCTCCGGAAC TTCATAATGC CACTAAGAAG CTCAAACTTC 
CATTATCATG AGCTCAAACA CGTGGTTATT GTGGGAGACC TGGAATATTT GAGGAAAGAG 
TGGAAGACGC TGTACAACTT GCCGAAAATC TCGATTTTGA ATGGCTCCCC GCTATCTCGA 
GCAGATTTAC GCGCCGTCAA CATTAACCTC TGCGACATGT GTGTCATAAT CTCTGCCCGG 
GTCCCAAATA CCGAAGATAC CACTTTGGCA GATAAGGAAG CGATTCTGGC ATCATTGAAC 
ATTAAAGCTA TGCAATTTGA TGATACCCTT GGCTTTTTCC CGATGAGGCA TCAAACTGGC 
GATCGGAGCC CGCTGGGCTC CCCGATTTCC ATGCAGAAGA AGGGCGCCAA GTTTGGGACC 
AATGTGCCGA TGATTACGGA GCTTGTTAAC GACTCAAATG TGCAATTCCT CGACCAGGAC 
GACGACGACG ATCCGGACAC CGAGCTCTAC CTCACGCAGC CCTTCGCCTG CGGAACTGCC 
TTCGCCATTT CAGTGTTGGA TTCTTTGATG AGTACTACCT ACTTCAACGA CTCGGCCCTG 
ACCCTCATCC GAACTCTGGT CACTGGTGGA GCCACACCGG AGCTTGAACT TATTCTGGCA 
GAAGGCGCCG GGCTCCGAGG TGGCTACAGT ACCCCGGAGA CCCTCTCAAA CAGAGATCGG 
TGCCGAATTG CTCAGATTTC GCTGCAGGAT AACCCGTACG ATGGAGTTGT GCACAACACT 
ACCTATGGTG CAATGTTCAC GATAGCTCTG AGGAGGTACG GCCAACTTTG TATCGGGCTG 
TATAGGCTTC ACGACCAGGA TAATCCGGAC TCTATGAAGC GATATGTTAT CACTAATCCT 
CCAGCAGAAT TGAGGATTAA AAATACTGAT TACGTCTACG TACTTGAACA ATTCGATCCG 
GGACTGGAGT ACGAGCCGGG CAAACGACAC TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269881.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256810.1 Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

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CloneID	OCa346287
Clone ID Related Accession (Same CDS sequence)	NM_001269881.1 , NM_001269881.2
Accession Version	NM_001269881.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Ion_trans domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269881.1.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGGCC ATCTTCCGGA GGACGCCGAA ATTCGATGAG TATTCCGCCA 
GACGGCAGGG GTGTCGATTT TAGCAAGGAT TTTGAGCAGC AATTCCAAGA CATGAAATAT 
GACAGTACTG GAATGTTCCA CTGGTGTCCA TCCCGTAACC TAGAGGACTG TGTTCTGGAG 
CGCCACCAGG CCGCTATGAC CGTACTCAAC GGCCACGTGG TGGTTTGTTT GTTCGCTGAT 
CAGGATTCTC CGCTAATTGG GCTCCGGAAC TTCATAATGC CACTAAGAAG CTCAAACTTC 
CATTATCATG AGCTCAAACA CGTGGTTATT GTGGGAGACC TGGAATATTT GAGGAAAGAG 
TGGAAGACGC TGTACAACTT GCCGAAAATC TCGATTTTGA ATGGCTCCCC GCTATCTCGA 
GCAGATTTAC GCGCCGTCAA CATTAACCTC TGCGACATGT GTGTCATAAT CTCTGCCCGG 
GTCCCAAATA CCGAAGATAC CACTTTGGCA GATAAGGAAG CGATTCTGGC ATCATTGAAC 
ATTAAAGCTA TGCAATTTGA TGATACCCTT GGCTTTTTCC CGATGAGGCA TCAAACTGGC 
GATCGGAGCC CGCTGGGCTC CCCGATTTCC ATGCAGAAGA AGGGCGCCAA GTTTGGGACC 
AATGTGCCGA TGATTACGGA GCTTGTTAAC GACTCAAATG TGCAATTCCT CGACCAGGAC 
GACGACGACG ATCCGGACAC CGAGCTCTAC CTCACGCAGC CCTTCGCCTG CGGAACTGCC 
TTCGCCATTT CAGTGTTGGA TTCTTTGATG AGTACTACCT ACTTCAACGA CTCGGCCCTG 
ACCCTCATCC GAACTCTGGT CACTGGTGGA GCCACACCGG AGCTTGAACT TATTCTGGCA 
GAAGGCGCCG GGCTCCGAGG TGGCTACAGT ACCCCGGAGA CCCTCTCAAA CAGAGATCGG 
TGCCGAATTG CTCAGATTTC GCTGCAGGAT AACCCGTACG ATGGAGTTGT GCACAACACT 
ACCTATGGTG CAATGTTCAC GATAGCTCTG AGGAGGTACG GCCAACTTTG TATCGGGCTG 
TATAGGCTTC ACGACCAGGA TAATCCGGAC TCTATGAAGC GATATGTTAT CACTAATCCT 
CCAGCAGAAT TGAGGATTAA AAATACTGAT TACGTCTACG TACTTGAACA ATTCGATCCG 
GGACTGGAGT ACGAGCCGGG CAAACGACAC TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256810.1
CDS	40..3435
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269881.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269881.2

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGGCC ATCTTCCGGA GGACGCCGAA ATTCGATGAG TATTCCGCCA 
GACGGCAGGG GTGTCGATTT TAGCAAGGAT TTTGAGCAGC AATTCCAAGA CATGAAATAT 
GACAGTACTG GAATGTTCCA CTGGTGTCCA TCCCGTAACC TAGAGGACTG TGTTCTGGAG 
CGCCACCAGG CCGCTATGAC CGTACTCAAC GGCCACGTGG TGGTTTGTTT GTTCGCTGAT 
CAGGATTCTC CGCTAATTGG GCTCCGGAAC TTCATAATGC CACTAAGAAG CTCAAACTTC 
CATTATCATG AGCTCAAACA CGTGGTTATT GTGGGAGACC TGGAATATTT GAGGAAAGAG 
TGGAAGACGC TGTACAACTT GCCGAAAATC TCGATTTTGA ATGGCTCCCC GCTATCTCGA 
GCAGATTTAC GCGCCGTCAA CATTAACCTC TGCGACATGT GTGTCATAAT CTCTGCCCGG 
GTCCCAAATA CCGAAGATAC CACTTTGGCA GATAAGGAAG CGATTCTGGC ATCATTGAAC 
ATTAAAGCTA TGCAATTTGA TGATACCCTT GGCTTTTTCC CGATGAGGCA TCAAACTGGC 
GATCGGAGCC CGCTGGGCTC CCCGATTTCC ATGCAGAAGA AGGGCGCCAA GTTTGGGACC 
AATGTGCCGA TGATTACGGA GCTTGTTAAC GACTCAAATG TGCAATTCCT CGACCAGGAC 
GACGACGACG ATCCGGACAC CGAGCTCTAC CTCACGCAGC CCTTCGCCTG CGGAACTGCC 
TTCGCCATTT CAGTGTTGGA TTCTTTGATG AGTACTACCT ACTTCAACGA CTCGGCCCTG 
ACCCTCATCC GAACTCTGGT CACTGGTGGA GCCACACCGG AGCTTGAACT TATTCTGGCA 
GAAGGCGCCG GGCTCCGAGG TGGCTACAGT ACCCCGGAGA CCCTCTCAAA CAGAGATCGG 
TGCCGAATTG CTCAGATTTC GCTGCAGGAT AACCCGTACG ATGGAGTTGT GCACAACACT 
ACCTATGGTG CAATGTTCAC GATAGCTCTG AGGAGGTACG GCCAACTTTG TATCGGGCTG 
TATAGGCTTC ACGACCAGGA TAATCCGGAC TCTATGAAGC GATATGTTAT CACTAATCCT 
CCAGCAGAAT TGAGGATTAA AAATACTGAT TACGTCTACG TACTTGAACA ATTCGATCCG 
GGACTGGAGT ACGAGCCGGG CAAACGACAC TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269878.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256807.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

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CloneID	OCa346288
Clone ID Related Accession (Same CDS sequence)	NM_001269878.1 , NM_001269878.2
Accession Version	NM_001269878.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256807.1
CDS	1..3483
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAAREKNAKISDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269878.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269878.1

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269878.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256807.1 Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

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CloneID	OCa346288
Clone ID Related Accession (Same CDS sequence)	NM_001269878.1 , NM_001269878.2
Accession Version	NM_001269878.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Ion_trans domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269878.1.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256807.1
CDS	40..3522
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAAREKNAKISDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269878.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269878.2

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269880.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256809.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

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CloneID	OCa346289
Clone ID Related Accession (Same CDS sequence)	NM_001269880.1 , NM_001269880.2
Accession Version	NM_001269880.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGTTC GACGAGTGAT ACTCATCTCA ACACGAAATC CCTTCGATTT 
GCCTATGAAA TCAAAAAACT CATGCCATCT TCCGGAGGAC GCCGAAATTC GATGAGTATT 
CCGCCAGACG GCAGGGGTGT CGATTTTAGC AAGGATTTTG AGCAGCAATT CCAAGACATG 
AAATATGACA GTACTGGAAT GTTCCACTGG TGTCCATCCC GTAACCTAGA GGACTGTGTT 
CTGGAGCGCC ACCAGGCCGC TATGACCGTA CTCAACGGCC ACGTGGTGGT TTGTTTGTTC 
GCTGATCAGG ATTCTCCGCT AATTGGGCTC CGGAACTTCA TAATGCCACT AAGAAGCTCA 
AACTTCCATT ATCATGAGCT CAAACACGTG GTTATTGTGG GAGACCTGGA ATATTTGAGG 
AAAGAGTGGA AGACGCTGTA CAACTTGCCG AAAATCTCGA TTTTGAATGG CTCCCCGCTA 
TCTCGAGCAG ATTTACGCGC CGTCAACATT AACCTCTGCG ACATGTGTGT CATAATCTCT 
GCCCGGGTCC CAAATACCGA AGATACCACT TTGGCAGATA AGGAAGCGAT TCTGGCATCA 
TTGAACATTA AAGCTATGCA ATTTGATGAT ACCCTTGGCT TTTTCCCGAT GAGGCATCAA 
ACTGGCGATC GGAGCCCGCT GGGCTCCCCG ATTTCCATGC AGAAGAAGGG CGCCAAGTTT 
GGGACCAATG TGCCGATGAT TACGGAGCTT GTTAACGACT CAAATGTGCA ATTCCTCGAC 
CAGGACGACG ACGACGATCC GGACACCGAG CTCTACCTCA CGCAGCCCTT CGCCTGCGGA 
ACTGCCTTCG CCATTTCAGT GTTGGATTCT TTGATGAGTA CTACCTACTT CAACGACTCG 
GCCCTGACCC TCATCCGAAC TCTGGTCACT GGTGGAGCCA CACCGGAGCT TGAACTTATT 
CTGGCAGAAG GCGCCGGGCT CCGAGGTGGC TACAGTACCC CGGAGACCCT CTCAAACAGA 
GATCGGTGCC GAATTGCTCA GATTTCGCTG CAGGATAACC CGTACGATGG AGTTGTGCAC 
AACACTACCT ATGGTGCAAT GTTCACGATA GCTCTGAGGA GGTACGGCCA ACTTTGTATC 
GGGCTGTATA GGCTTCACGA CCAGGATAAT CCGGACTCTA TGAAGCGATA TGTTATCACT 
AATCCTCCAG CAGAATTGAG GATTAAAAAT ACTGATTACG TCTACGTACT TGAACAATTC 
GATCCGGGAC TGGAGTACGA GCCGGGCAAA CGACACTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256809.1
CDS	1..3462
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269880.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269880.1

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGTTC GACGAGTGAT ACTCATCTCA ACACGAAATC CCTTCGATTT 
GCCTATGAAA TCAAAAAACT CATGCCATCT TCCGGAGGAC GCCGAAATTC GATGAGTATT 
CCGCCAGACG GCAGGGGTGT CGATTTTAGC AAGGATTTTG AGCAGCAATT CCAAGACATG 
AAATATGACA GTACTGGAAT GTTCCACTGG TGTCCATCCC GTAACCTAGA GGACTGTGTT 
CTGGAGCGCC ACCAGGCCGC TATGACCGTA CTCAACGGCC ACGTGGTGGT TTGTTTGTTC 
GCTGATCAGG ATTCTCCGCT AATTGGGCTC CGGAACTTCA TAATGCCACT AAGAAGCTCA 
AACTTCCATT ATCATGAGCT CAAACACGTG GTTATTGTGG GAGACCTGGA ATATTTGAGG 
AAAGAGTGGA AGACGCTGTA CAACTTGCCG AAAATCTCGA TTTTGAATGG CTCCCCGCTA 
TCTCGAGCAG ATTTACGCGC CGTCAACATT AACCTCTGCG ACATGTGTGT CATAATCTCT 
GCCCGGGTCC CAAATACCGA AGATACCACT TTGGCAGATA AGGAAGCGAT TCTGGCATCA 
TTGAACATTA AAGCTATGCA ATTTGATGAT ACCCTTGGCT TTTTCCCGAT GAGGCATCAA 
ACTGGCGATC GGAGCCCGCT GGGCTCCCCG ATTTCCATGC AGAAGAAGGG CGCCAAGTTT 
GGGACCAATG TGCCGATGAT TACGGAGCTT GTTAACGACT CAAATGTGCA ATTCCTCGAC 
CAGGACGACG ACGACGATCC GGACACCGAG CTCTACCTCA CGCAGCCCTT CGCCTGCGGA 
ACTGCCTTCG CCATTTCAGT GTTGGATTCT TTGATGAGTA CTACCTACTT CAACGACTCG 
GCCCTGACCC TCATCCGAAC TCTGGTCACT GGTGGAGCCA CACCGGAGCT TGAACTTATT 
CTGGCAGAAG GCGCCGGGCT CCGAGGTGGC TACAGTACCC CGGAGACCCT CTCAAACAGA 
GATCGGTGCC GAATTGCTCA GATTTCGCTG CAGGATAACC CGTACGATGG AGTTGTGCAC 
AACACTACCT ATGGTGCAAT GTTCACGATA GCTCTGAGGA GGTACGGCCA ACTTTGTATC 
GGGCTGTATA GGCTTCACGA CCAGGATAAT CCGGACTCTA TGAAGCGATA TGTTATCACT 
AATCCTCCAG CAGAATTGAG GATTAAAAAT ACTGATTACG TCTACGTACT TGAACAATTC 
GATCCGGGAC TGGAGTACGA GCCGGGCAAA CGACACTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269880.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256809.1 Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

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CloneID	OCa346289
Clone ID Related Accession (Same CDS sequence)	NM_001269880.1 , NM_001269880.2
Accession Version	NM_001269880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Ion_trans domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269880.1.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGTTC GACGAGTGAT ACTCATCTCA ACACGAAATC CCTTCGATTT 
GCCTATGAAA TCAAAAAACT CATGCCATCT TCCGGAGGAC GCCGAAATTC GATGAGTATT 
CCGCCAGACG GCAGGGGTGT CGATTTTAGC AAGGATTTTG AGCAGCAATT CCAAGACATG 
AAATATGACA GTACTGGAAT GTTCCACTGG TGTCCATCCC GTAACCTAGA GGACTGTGTT 
CTGGAGCGCC ACCAGGCCGC TATGACCGTA CTCAACGGCC ACGTGGTGGT TTGTTTGTTC 
GCTGATCAGG ATTCTCCGCT AATTGGGCTC CGGAACTTCA TAATGCCACT AAGAAGCTCA 
AACTTCCATT ATCATGAGCT CAAACACGTG GTTATTGTGG GAGACCTGGA ATATTTGAGG 
AAAGAGTGGA AGACGCTGTA CAACTTGCCG AAAATCTCGA TTTTGAATGG CTCCCCGCTA 
TCTCGAGCAG ATTTACGCGC CGTCAACATT AACCTCTGCG ACATGTGTGT CATAATCTCT 
GCCCGGGTCC CAAATACCGA AGATACCACT TTGGCAGATA AGGAAGCGAT TCTGGCATCA 
TTGAACATTA AAGCTATGCA ATTTGATGAT ACCCTTGGCT TTTTCCCGAT GAGGCATCAA 
ACTGGCGATC GGAGCCCGCT GGGCTCCCCG ATTTCCATGC AGAAGAAGGG CGCCAAGTTT 
GGGACCAATG TGCCGATGAT TACGGAGCTT GTTAACGACT CAAATGTGCA ATTCCTCGAC 
CAGGACGACG ACGACGATCC GGACACCGAG CTCTACCTCA CGCAGCCCTT CGCCTGCGGA 
ACTGCCTTCG CCATTTCAGT GTTGGATTCT TTGATGAGTA CTACCTACTT CAACGACTCG 
GCCCTGACCC TCATCCGAAC TCTGGTCACT GGTGGAGCCA CACCGGAGCT TGAACTTATT 
CTGGCAGAAG GCGCCGGGCT CCGAGGTGGC TACAGTACCC CGGAGACCCT CTCAAACAGA 
GATCGGTGCC GAATTGCTCA GATTTCGCTG CAGGATAACC CGTACGATGG AGTTGTGCAC 
AACACTACCT ATGGTGCAAT GTTCACGATA GCTCTGAGGA GGTACGGCCA ACTTTGTATC 
GGGCTGTATA GGCTTCACGA CCAGGATAAT CCGGACTCTA TGAAGCGATA TGTTATCACT 
AATCCTCCAG CAGAATTGAG GATTAAAAAT ACTGATTACG TCTACGTACT TGAACAATTC 
GATCCGGGAC TGGAGTACGA GCCGGGCAAA CGACACTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256809.1
CDS	40..3501
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTPDWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269880.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269880.2

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCACAAACC ACACCGGACT GGCTGAACCT GTACCTTTGC 
GGTGCCGGAA TGGAAATGTA CACCGACACG CTATCCCACT CGTTCGTCGG AATGACTTTT 
CCTGAAGCTG TCGACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGATTAT 
TCGGACTTTG ACGCTCTGTT TTATCAGAAT GACGCAAGAG CCACCGATGT GCTCCAGCAG 
TTCCAGCCAC AAGCGCCAGC AGGTCCGATG GGCCATTTGG GCCAACAAGT TCAGCTAAGG 
ATGATTAATC AACAGAGTTC GACGAGTGAT ACTCATCTCA ACACGAAATC CCTTCGATTT 
GCCTATGAAA TCAAAAAACT CATGCCATCT TCCGGAGGAC GCCGAAATTC GATGAGTATT 
CCGCCAGACG GCAGGGGTGT CGATTTTAGC AAGGATTTTG AGCAGCAATT CCAAGACATG 
AAATATGACA GTACTGGAAT GTTCCACTGG TGTCCATCCC GTAACCTAGA GGACTGTGTT 
CTGGAGCGCC ACCAGGCCGC TATGACCGTA CTCAACGGCC ACGTGGTGGT TTGTTTGTTC 
GCTGATCAGG ATTCTCCGCT AATTGGGCTC CGGAACTTCA TAATGCCACT AAGAAGCTCA 
AACTTCCATT ATCATGAGCT CAAACACGTG GTTATTGTGG GAGACCTGGA ATATTTGAGG 
AAAGAGTGGA AGACGCTGTA CAACTTGCCG AAAATCTCGA TTTTGAATGG CTCCCCGCTA 
TCTCGAGCAG ATTTACGCGC CGTCAACATT AACCTCTGCG ACATGTGTGT CATAATCTCT 
GCCCGGGTCC CAAATACCGA AGATACCACT TTGGCAGATA AGGAAGCGAT TCTGGCATCA 
TTGAACATTA AAGCTATGCA ATTTGATGAT ACCCTTGGCT TTTTCCCGAT GAGGCATCAA 
ACTGGCGATC GGAGCCCGCT GGGCTCCCCG ATTTCCATGC AGAAGAAGGG CGCCAAGTTT 
GGGACCAATG TGCCGATGAT TACGGAGCTT GTTAACGACT CAAATGTGCA ATTCCTCGAC 
CAGGACGACG ACGACGATCC GGACACCGAG CTCTACCTCA CGCAGCCCTT CGCCTGCGGA 
ACTGCCTTCG CCATTTCAGT GTTGGATTCT TTGATGAGTA CTACCTACTT CAACGACTCG 
GCCCTGACCC TCATCCGAAC TCTGGTCACT GGTGGAGCCA CACCGGAGCT TGAACTTATT 
CTGGCAGAAG GCGCCGGGCT CCGAGGTGGC TACAGTACCC CGGAGACCCT CTCAAACAGA 
GATCGGTGCC GAATTGCTCA GATTTCGCTG CAGGATAACC CGTACGATGG AGTTGTGCAC 
AACACTACCT ATGGTGCAAT GTTCACGATA GCTCTGAGGA GGTACGGCCA ACTTTGTATC 
GGGCTGTATA GGCTTCACGA CCAGGATAAT CCGGACTCTA TGAAGCGATA TGTTATCACT 
AATCCTCCAG CAGAATTGAG GATTAAAAAT ACTGATTACG TCTACGTACT TGAACAATTC 
GATCCGGGAC TGGAGTACGA GCCGGGCAAA CGACACTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269874.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256803.1 Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

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CloneID	OCa346290
Clone ID Related Accession (Same CDS sequence)	NM_001269874.2 , NM_001269874.1
Accession Version	NM_001269874.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Ion_trans domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269874.1.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256803.1
CDS	40..3456
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAAREKNAKISDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269874.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269874.2

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269874.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256803.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

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CloneID	OCa346290
Clone ID Related Accession (Same CDS sequence)	NM_001269874.2 , NM_001269874.1
Accession Version	NM_001269874.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256803.1
CDS	1..3417
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAAREKNAKISDYSDFDALFYQNDARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269874.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269874.1

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGAAAAA 
AATGCCAAAA TTTCAGATTA TTCGGACTTT GACGCTCTGT TTTATCAGAA TGACGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGGC CATCTTCCGG AGGACGCCGA 
AATTCGATGA GTATTCCGCC AGACGGCAGG GGTGTCGATT TTAGCAAGGA TTTTGAGCAG 
CAATTCCAAG ACATGAAATA TGACAGTACT GGAATGTTCC ACTGGTGTCC ATCCCGTAAC 
CTAGAGGACT GTGTTCTGGA GCGCCACCAG GCCGCTATGA CCGTACTCAA CGGCCACGTG 
GTGGTTTGTT TGTTCGCTGA TCAGGATTCT CCGCTAATTG GGCTCCGGAA CTTCATAATG 
CCACTAAGAA GCTCAAACTT CCATTATCAT GAGCTCAAAC ACGTGGTTAT TGTGGGAGAC 
CTGGAATATT TGAGGAAAGA GTGGAAGACG CTGTACAACT TGCCGAAAAT CTCGATTTTG 
AATGGCTCCC CGCTATCTCG AGCAGATTTA CGCGCCGTCA ACATTAACCT CTGCGACATG 
TGTGTCATAA TCTCTGCCCG GGTCCCAAAT ACCGAAGATA CCACTTTGGC AGATAAGGAA 
GCGATTCTGG CATCATTGAA CATTAAAGCT ATGCAATTTG ATGATACCCT TGGCTTTTTC 
CCGATGAGGC ATCAAACTGG CGATCGGAGC CCGCTGGGCT CCCCGATTTC CATGCAGAAG 
AAGGGCGCCA AGTTTGGGAC CAATGTGCCG ATGATTACGG AGCTTGTTAA CGACTCAAAT 
GTGCAATTCC TCGACCAGGA CGACGACGAC GATCCGGACA CCGAGCTCTA CCTCACGCAG 
CCCTTCGCCT GCGGAACTGC CTTCGCCATT TCAGTGTTGG ATTCTTTGAT GAGTACTACC 
TACTTCAACG ACTCGGCCCT GACCCTCATC CGAACTCTGG TCACTGGTGG AGCCACACCG 
GAGCTTGAAC TTATTCTGGC AGAAGGCGCC GGGCTCCGAG GTGGCTACAG TACCCCGGAG 
ACCCTCTCAA ACAGAGATCG GTGCCGAATT GCTCAGATTT CGCTGCAGGA TAACCCGTAC 
GATGGAGTTG TGCACAACAC TACCTATGGT GCAATGTTCA CGATAGCTCT GAGGAGGTAC 
GGCCAACTTT GTATCGGGCT GTATAGGCTT CACGACCAGG ATAATCCGGA CTCTATGAAG 
CGATATGTTA TCACTAATCC TCCAGCAGAA TTGAGGATTA AAAATACTGA TTACGTCTAC 
GTACTTGAAC AATTCGATCC GGGACTGGAG TACGAGCCGG GCAAACGACA CTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269876.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256805.1 Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

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CloneID	OCa346291
Clone ID Related Accession (Same CDS sequence)	NM_001269876.2 , NM_001269876.1
Accession Version	NM_001269876.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Ion_trans domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269876.1.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256805.1
CDS	40..3462
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269876.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Ion_trans domain-containing protein (slo-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269876.2

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slo-1 ( NM_001269876.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256805.1 Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Price & Availability↑

CloneID	OCa346291
Clone ID Related Accession (Same CDS sequence)	NM_001269876.2 , NM_001269876.1
Accession Version	NM_001269876.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Calcium-activated potassium channel slo-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256805.1
CDS	1..3423
Translation	MGEIYSPSQSKGFNQPYGYPMNCNLSRVFMEMTEEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSETDPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKGFINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQSSTSDTHLNTKSLRFAYEIKKLMPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWCPSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTEDTTLADKEAILASLNIKAMQFDDTLGFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRDRCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYRLHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQFDPGLEYEPGKRHF

Target ORF information:

RefSeq Version	NM_001269876.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calcium-activated potassium channel slo-1 (slo-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269876.1

ORF Insert Sequence:

ATGGGCGAGA TTTACTCGCC TTCGCAGTCG AAGGGCTTCA ATCAACCATA TGGATACCCG 
ATGAATTGTA ACCTTAGTCG AGTATTCATG GAGATGACTG AAGAAGATCG AAAGTGCCTG 
GAGGAGCGCA AATATTGGTG TTTTTTACTG AGCAGCATCA CCACATTCTG CGCCTCAATG 
ATATTGGTCG TAATATGGCG AGTGGTGACA CACCTGTGCT GTCAACGCCG AGAAAAAGAG 
TTTGTGGAGC CCATACCTGC TCCGGAAGCC GTCCAAATAA ATATGAACGG GTCGAAGCAC 
GCGCCAAGTG AAACCGACCC GTTTTTGAAG CAACAAGAGG AAAAACACTT GGGATGGATG 
ACAGAAGCCA AGGACTGGGC TGGCGAGCTG ATATCCGGAC AAAGTCTGAC GGGACGGTTT 
CTGGTACTTT TGGTGTTCAT TTTATCGATT GGATCGTTGA TTATTTATTT TTACGACGCT 
AGTTTCCAAA ACTTCCAAGT AGAAACGTGC ATCCCGTGGC AGGACAGTCC CTCGCAACAA 
ATCGACCTGG GCTTCAACAT ATTTTTTCTC GTCTATTTTT TCATTAGATT CATCGCCGCG 
TCCGACAAAG TTTGGTTTCT GCTTGAAATG TACAGCTGGA TTGATTTTTT CACGATTCCG 
CCAAGCTTCG TGGCAATTTA CTTGCAGAGG AATTGGCTCG GATTCCGTTT CCTCCGTGCT 
CTTCGCCTCA TGACCGTACC CGACATTCTA CAGTACCTCA ACATCCTGAA AACATCTTCA 
TCAATCCGAT TGACACAGTT GGTCACAATT TTCGTGGCGG TTTGTCTGAC TGGAGCCGGC 
TTGGTGCACT TACTGGAAAA TTCTGGGGAC TTCTTCAAAG GATTTATAAA TCCGCACAGA 
ATCACTTATG CGGACTCTGT GTACTTTGTA CTGGTCACAA TGTCTACTGT CGGATATGGA 
GATATCTATT GTACGACGCT TTGCGGCCGA CTTTTCATGA TTTTCTTCAT TCTGTTCGGT 
TTGGCCATGT TTGCAAGTTA CGTACCAGAA ATTGCCGATT TGATTGGAAA CCGGCAAAAA 
TACGGTGGGG AGTACAAAGG AGAGCACGGG AAGAAGCACA TAGTGGTCTG TGGCCATATC 
ACCTACGATT CGGTGTCCCA TTTTCTTCAA GATTTCCTAC ACGAGGACCG TGATGACGTG 
GATGTCGAAG TGGTGTTTTT GCATCGTGTC GTGCCGGATT TGGAGCTGGA AGGCTTGTTT 
AAGCGGCATT TCACAAAAGT CGAATTTTTC ACGGGGACTG TCATGGATTC TCTGGATCTT 
AGCAGAGTCA AGATCGGGGA CGCCGACGCC TGCCTGGTCC TTGCCAACAA GTACAGTACC 
AATCCGGACG CCGAGGACGC CGCAAACATT ATGCGTGTAA TTTCGATCAA AAACTACTCA 
TCGGACATCA GAGTTATTGT GCAACTAATG CAATATCACA ATAAGGCCTA CCTTCTAAAT 
ATCCCCTCCT GGGACTGGAA ACGAGGTGAT GACGTCATCT GTCTCGCGGA GCTTAAGCTC 
GGATTCATTG CTCAAAGCTG TCTGGCACCC GGATTCTCTA CGATGATGGC TAATTTATTT 
GCAATGAGAA GTTTCAAAAC GTCTCCTCAC ACCCCTCTCT GGCTTAACGA CTACTTACGT 
GGTGCCGGAA TGGAAATGTA CACGGAAAGT CTGAGTCCTT CGTTTGCGAA TATGTCATTT 
CCAGAAGCTG CAAACCTATT ATTCAACCGC CTGGGGCTTC TTCTTCTGGC GATTGAGCTT 
AAAGATGAGG AAAATAAAGA ATGCAATATT GCAATCAATC CAGGCCCACA TATTGTCATC 
CAGCCTCAAA CTCAAGGATT TTTCATTGCT CAGAGCGCTG ACGAGGTGAA GCGGGCGTTT 
TTCTGGTGTA AGCAGTGTCA TGACGATATT AAGGACGTTT CCTTGATCAA AAAATGTAAA 
TGCAAAAATT TGGCCCTCTT CCGTCGCAAC ACCAAACACT CGACAGCGGC CCGTGCAAGA 
GCCACCGATG TGCTCCAGCA GTTCCAGCCA CAAGCGCCAG CAGGTCCGAT GGGCCATTTG 
GGCCAACAAG TTCAGCTAAG GATGATTAAT CAACAGAGTT CGACGAGTGA TACTCATCTC 
AACACGAAAT CCCTTCGATT TGCCTATGAA ATCAAAAAAC TCATGCCATC TTCCGGAGGA 
CGCCGAAATT CGATGAGTAT TCCGCCAGAC GGCAGGGGTG TCGATTTTAG CAAGGATTTT 
GAGCAGCAAT TCCAAGACAT GAAATATGAC AGTACTGGAA TGTTCCACTG GTGTCCATCC 
CGTAACCTAG AGGACTGTGT TCTGGAGCGC CACCAGGCCG CTATGACCGT ACTCAACGGC 
CACGTGGTGG TTTGTTTGTT CGCTGATCAG GATTCTCCGC TAATTGGGCT CCGGAACTTC 
ATAATGCCAC TAAGAAGCTC AAACTTCCAT TATCATGAGC TCAAACACGT GGTTATTGTG 
GGAGACCTGG AATATTTGAG GAAAGAGTGG AAGACGCTGT ACAACTTGCC GAAAATCTCG 
ATTTTGAATG GCTCCCCGCT ATCTCGAGCA GATTTACGCG CCGTCAACAT TAACCTCTGC 
GACATGTGTG TCATAATCTC TGCCCGGGTC CCAAATACCG AAGATACCAC TTTGGCAGAT 
AAGGAAGCGA TTCTGGCATC ATTGAACATT AAAGCTATGC AATTTGATGA TACCCTTGGC 
TTTTTCCCGA TGAGGCATCA AACTGGCGAT CGGAGCCCGC TGGGCTCCCC GATTTCCATG 
CAGAAGAAGG GCGCCAAGTT TGGGACCAAT GTGCCGATGA TTACGGAGCT TGTTAACGAC 
TCAAATGTGC AATTCCTCGA CCAGGACGAC GACGACGATC CGGACACCGA GCTCTACCTC 
ACGCAGCCCT TCGCCTGCGG AACTGCCTTC GCCATTTCAG TGTTGGATTC TTTGATGAGT 
ACTACCTACT TCAACGACTC GGCCCTGACC CTCATCCGAA CTCTGGTCAC TGGTGGAGCC 
ACACCGGAGC TTGAACTTAT TCTGGCAGAA GGCGCCGGGC TCCGAGGTGG CTACAGTACC 
CCGGAGACCC TCTCAAACAG AGATCGGTGC CGAATTGCTC AGATTTCGCT GCAGGATAAC 
CCGTACGATG GAGTTGTGCA CAACACTACC TATGGTGCAA TGTTCACGAT AGCTCTGAGG 
AGGTACGGCC AACTTTGTAT CGGGCTGTAT AGGCTTCACG ACCAGGATAA TCCGGACTCT 
ATGAAGCGAT ATGTTATCAC TAATCCTCCA GCAGAATTGA GGATTAAAAA TACTGATTAC 
GTCTACGTAC TTGAACAATT CGATCCGGGA CTGGAGTACG AGCCGGGCAA ACGACACTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	slo-1
Entrez Gene ID	180203
Full Name	Calcium-activated potassium channel slo-1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

Pan troglodytes (chimpanzee)	KCNMA1	XP_003312698.2
Mus musculus (house mouse)	Kcnma1	NP_001240293.1
Gallus gallus (chicken)	KCNMA1	NP_989555.1
Bos taurus (cattle)	KCNMA1	NP_777105.1
Danio rerio (zebrafish)	kcnma1a	NP_001139072.1
Drosophila melanogaster (fruit fly)	slo	NP_001014652.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003709	XP_003436577.1
Canis lupus familiaris (dog)	KCNMA1	NP_001003300.1
Rattus norvegicus (Norway rat)	Kcnma1	NP_114016.1
Homo sapiens (human)	KCNMA1	NP_001154824.1
Caenorhabditis elegans (roundworm)	slo-1	NP_001024259.1
Xenopus tropicalis (tropical clawed frog)	kcnma1	XP_004916071.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

slo-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

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Target ORF information:

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Target ORF information:

Target ORF information:

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