twk-11 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	twk-11
Entrez Gene ID	179088
Full Name	TWiK family of potassium channels
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_182339.2	NP_872139.2	TWiK family of potassium channels
NM_072381.5	NP_504782.2	TWiK family of potassium channels
NM_001356689.1	NP_001343632.1	TWiK family of potassium channels
NM_001356689.2	NP_001343632.1	TWiK family of potassium channels
NM_072382.6	NP_504783.2	TWiK family of potassium channels
NM_072382.7	NP_504783.2	TWiK family of potassium channels
NM_182339.3	NP_872139.2	TWiK family of potassium channels

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following twk-11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the twk-11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa347228	NM_182339.3 Latest version!	Caenorhabditis elegans TWiK family of potassium channels (twk-11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa529871	NM_001356689.2 Latest version!	Caenorhabditis elegans TWiK family of potassium channels (twk-11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCa347226	NM_072381.5 Latest version!	Caenorhabditis elegans TWiK family of potassium channels (twk-11), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa347227	NM_072382.7 Latest version!	Caenorhabditis elegans TWiK family of potassium channels (twk-11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa529871	NM_001356689.1	Caenorhabditis elegans TWiK family of potassium channels (twk-11), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa347227	NM_072382.6	Caenorhabditis elegans TWiK family of potassium channels (twk-11), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa347228	NM_182339.2	Caenorhabditis elegans TWiK family of potassium channels (twk-11), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

twk-11 ( NM_182339.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_872139.2 Caenorhabditis elegans TWiK family of potassium channels (twk-11), partial mRNA.

Price & Availability↑

CloneID	OCa347228
Clone ID Related Accession (Same CDS sequence)	NM_182339.2 , NM_182339.3
Accession Version	NM_182339.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	TWiK family of potassium channels
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Oct 5, 2006 this sequence version replaced NM_182339.1. COMPLETENESS: incomplete on both ends.

ATGTCCAATG ATAACTTGGC GATTGAAATC GACCCTCCGG ACGATGTGGA GTATGATGTG 
GAAGCAGGCT TCAACTCTTC CACATGCCAT TTAATGCACA ATTTCGAAGA GGAACGTCCA 
ACCTCTCTCG TAGCCTCCAT AAAGGAAAGT GCCATTAGCT CAAAGCGAAA ACTAAAATAC 
TACTGCTCGT TCATATGCAC CCCAATCAAG AAGTTGTTAT CCACTTTCAA ACTACTCATC 
ATAATCGGTC TTTACTCATT TATTGGCGCT CATATATTCA TGTACTTGGA AGTTCCAACT 
GATTTAGAAG CAAGAGAAGA TGGATTTCAT CAAAGAAAAA TAGCGAGGGA AGTGATGGTG 
CTGAATCTCC GTGCAATTTT CTACGACAAC CGCGAAGATC GCGAGGAACG ATGGAAGCAT 
GCCATTTTGA AGTTCGAAGA GGACATTGGC TTAGAAGAAC CTGTCATTGA AACTGTATGG 
ACTTTTTGGA TGTCTTTCCT CTATGCAGGA ACTATTTTCA CAACAATAGG ATATGGAAAC 
ATTGCATGTA AGACGCGTGC TGGCCAGATA GCTACAATGG TATATGCTTT TGTCGGAATT 
CCGATCATGC TGGTGATGCT CACCTCGCTC AACAATTTTT TGTTAAAATG GATAAAACTT 
ATTACGAATG GAGTTAGTGA TATGACGCTT TATATTGGTG TTCGCTTGGG AATAACAGTT 
ATTCGGCAGG ACGAAGTCCA GAAGCGCCTT CGCTACACAA AACTTGCCAA GACAATGAAA 
AGGTGGAAAC TTTCAAAACA CGGAGCTCCA AGTAGTATAG CGATTAGCAA TTCGGAAGAA 
AACCGGTTAA ATTCAACACC TGAAGACGAC GAAGAAGAGG AAGAAATTCA TCAGGATCCT 
CCAGTTCTTT CGACTCTAAT TGCAACTGTT GCCTGGATTA TTCTTTCGGC TGCTGTGTTT 
TGTCTTTTTG AGGACTGGAC ATTTTTCACC AGTTTTTATT TTTGTTTCAT CTCTCTGACA 
ACAATCGGTC TTGGAGATGT GACACCTGCT AATCCAGAAT ATATGATTGC AACATTTGGA 
GTTGTGATTG TCGGTTTATC AATGCTCACT GTCTGTATCG ACGTCTTACA AGAAAAACTT 
GCTCAAATGT ATATGGCTCT ACTTCAGAAG TTATTGACAG AGTACATGGA AGCTGTGAAA 
AGCGGAGACC CAAATGCTGC ATCAGCTATG ATGGCTGGTT TTCAAGGAAG GGCAAAGTTT 
TTGATGCCGT TGATTAGTAA AAACGAAGGA GCAAAAGTTA TGGATAAGTT CAAACAAGAT 
TGTTCAAAGA AGGGAATCGA TCCACCACCG GTTTTAACAA ATATCAACCC GGAGACCGGA 
ATGCCAGCTT TTGCAAATGC TCCGAAAGAA GATTTTAGAG ATTATATTGA AGTTGCAGAT 
GAAAAATTTG CTGATGAACA ACAGAGGCTC TCTCAAACCC CGTTATCAAA ATCCACCGAC 
TTTTTACAAT CACCCACACC ACGTCCAACT GTCACTGTAG CTGTTACTCA AACAGCTAGA 
CCACAGGCAT GCGTATCACT AGAAGTACAG GCTGGAACCA CAATGGGAAA GTTCCATGAT 
TATGGATCAC AGACACAGAC GGAAAGTTCT GACGAAGGAA TTCAGACGGA TTATAGCATT 
GATTTTGATG ATGAAAAAAA CAAGGAGCCA GAGAAAGTTA TGGCGGACAT TGGCACTCAA 
TATGAAATCG TTCTATTAAC AAGTATTGGA ATTCAACCAG AAGTCTCATC AATTTATATT 
GGAGCCGGCT TATCTGATCA TTCGGACGAA GAAAGTGGAT GTGAATCAGA AGACGATTCG 
GATGACTCGG ATGTATCATC CGATGAGGAA AGTGAACTTA AATCTTCTGC GAAATACAAA 
GAAACACCTC TTCCATTTGA AGTCGAAGAT CCAGGAATGA TGTCGCTCGG ATTACAGTCT 
CCACCAATTG AAATAACTCC CTTCACGGAT GATGAATCAG CTGCAAGCGA CGCAAGAAGT 
TCTCCTGTCC CACCAACATC TTCCAAGCGA TCCAAGTTGA CACCAAAGAC TATAAAAGTT 
GGCAAATCGT TATCAATGGA CTCGACTGAT ACAGTTATTC ATGTGAAACC AGTTGGCGTT 
AATGATGATG AGAAAAGAAG TGAAGAAAAA GAAAGTAAAG ATCAAGAAAC AAATGATGAC 
CTAAAAACTG AAAGTGGACA GGAGAAAAAG AGAAGAAAGA AAAAGAAGAA GCTTCGTTCA 
AAGAAGACAA GAGAGCATGA GCTGAGATTT TTGAAGAATA GCGGAAATCA AGCATCTCCC 
CTTCTTATGG ATTGCTCTAC ACAATATGAG ATATTTACAG TGGAAAGTGG GTCGCAATAC 
GATGTGAAAG GAAGAAGCCG AAAATGTCAG ACAAAACTTT CCAGTATAAA TCATGAGCAG 
TTACAAGGGA TTCGAGAAAC AGTTCAAAAG TTGAGAAGAA GAGAAAGTGA AATGAGTAAG 
GTGTCGAATG TTTCTGAAAG TGACTGGGAT AGTGAAACTG GAAAAGAACC GGAAGTCGCT 
GAAATCAGTT TAGATCTTTC TGTAGTTGCT CTCGGAGTTG ATGAATCCGG GAAAGTTGTT 
GATATTCCTA AGGAAGTACC TAAAGCTGTT CAACGACATG AATCATTTTC ACCAACATCG 
GAGCTCACCA CTGCGTCCGA ATCACTTTCC GCTGAGAAAG TACATCTGGA TTCAGAAGAT 
GAGGAGTTAG AAAGTCTTGA AGAACTCGAA CTGTATACCA CAGACGCCGG AATTCAGACT 
GAAACAAAGG TCATGCTGGA CAAGCAAGAT AATGCAACTC CAAAGTTGCT GGAAATTATT 
AAAAACTGCA GCACTCAGAC AACATTCAGC ACATTCCACA TTCCGCAGAA GGTATATGAG 
TCGGCGAGCA CCTCAAGTGA CGAGGAAGAT CATAAAAACG ATGGTGAAGA TGAAAAACAG 
AAGAAATCTA AACAGGAAGT GTTCTTTATG ACCAACACTG GAGTACAGAC GATGCATACA 
TCGTTCCATA TTGATGAAAA ACCGAAATTT GTTGAAGAAA ATCTTCAAAC TCAAAATACC 
CACGACGGCA TAAAAGAACA AAGTGCCGTT GATGAAGAAA TGCCTCTGTT CTTAAAAAAT 
TCGGATTGTC AAACCGTTGA AGTAATATCA TTCGAATGTG CCACATCAAC AGAGAATGTT 
AGTTTTGTTG GAGACGTTGA AACTTCTGAA GGATCCAGTC AGACAGCCAT GCAAGTTTCT 
GAAACTGAAG TACAAACAAT GCAACAAGAA TTTGTGGTAG CAACAATGCA AACCGACGAG 
CCACTGTTGG TATCTGATGA GTCACAGACT GAGCAAACAA AATCTTCGGA AATTGATTCT 
CAAACTGATC CACCATCCAC AAAATCATTT ATATGTCAGA CAGAATTGAT AACAAGCTCG 
GAAGTTGAAG TTCAAGCAGT TCGAGCTGTA GAGTTTTCAC TTGTCGAATG CCAAACAGAT 
GCGCTAAATC ATGAAGAAGT AGAAGTTCAA ACAGAACTGG ATATTTCGGT TTCGCAGTCC 
CAAACTGATA ATGTTGATGT AGGCCACATT GAAATGCAAA CTGATATGAC AATAACAAAT 
GCATCGGAAT CTCAAACCGA TGTGCTCAGT GTAGGGGACA CGGAGATGCA AACAGACGAG 
AAAGAAATAT TCACAATAGA ATCGCAAACC GAACAACCAT CCACAGAACT GCAAGAGACT 
CAAACTGACG ATGTTTCTTT TGCTGGCGAT GTTCCAACAA CCGACCAAAT CACACAATCG 
GAATCTGCTC AAGTTTTCGA TTCTGAAACT CAATATGCTT TATCCATAGA AGAAAAGGAC 
GTCCAGGCAA CGCCAATCTC ATTTGAAACT CAGACACAAC ATTCCATTGA ACTTGAAGTG 
AAATCTTGCC AAACCGAAGG AGTTCGAATG AAAAACAAAA AGAGTCAGGT ATTCTGGAAT 
GCTAATTCAA TTGATTTACC AATAATCTTT ATTCGTGTGA TTCCCTATTT CCAAAAAATT 
TGTTTAGGTA GAAGACGACT CAATGCTGTC AGTTCTCAAA ATAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_872139.2
CDS	1..4128
Translation	MSNDNLAIEIDPPDDVEYDVEAGFNSSTCHLMHNFEEERPTSLVASIKESAISSKRKLKYYCSFICTPIKKLLSTFKLLIIIGLYSFIGAHIFMYLEVPTDLEAREDGFHQRKIAREVMVLNLRAIFYDNREDREERWKHAILKFEEDIGLEEPVIETVWTFWMSFLYAGTIFTTIGYGNIACKTRAGQIATMVYAFVGIPIMLVMLTSLNNFLLKWIKLITNGVSDMTLYIGVRLGITVIRQDEVQKRLRYTKLAKTMKRWKLSKHGAPSSIAISNSEENRLNSTPEDDEEEEEIHQDPPVLSTLIATVAWIILSAAVFCLFEDWTFFTSFYFCFISLTTIGLGDVTPANPEYMIATFGVVIVGLSMLTVCIDVLQEKLAQMYMALLQKLLTEYMEAVKSGDPNAASAMMAGFQGRAKFLMPLISKNEGAKVMDKFKQDCSKKGIDPPPVLTNINPETGMPAFANAPKEDFRDYIEVADEKFADEQQRLSQTPLSKSTDFLQSPTPRPTVTVAVTQTARPQACVSLEVQAGTTMGKFHDYGSQTQTESSDEGIQTDYSIDFDDEKNKEPEKVMADIGTQYEIVLLTSIGIQPEVSSIYIGAGLSDHSDEESGCESEDDSDDSDVSSDEESELKSSAKYKETPLPFEVEDPGMMSLGLQSPPIEITPFTDDESAASDARSSPVPPTSSKRSKLTPKTIKVGKSLSMDSTDTVIHVKPVGVNDDEKRSEEKESKDQETNDDLKTESGQEKKRRKKKKKLRSKKTREHELRFLKNSGNQASPLLMDCSTQYEIFTVESGSQYDVKGRSRKCQTKLSSINHEQLQGIRETVQKLRRRESEMSKVSNVSESDWDSETGKEPEVAEISLDLSVVALGVDESGKVVDIPKEVPKAVQRHESFSPTSELTTASESLSAEKVHLDSEDEELESLEELELYTTDAGIQTETKVMLDKQDNATPKLLEIIKNCSTQTTFSTFHIPQKVYESASTSSDEEDHKNDGEDEKQKKSKQEVFFMTNTGVQTMHTSFHIDEKPKFVEENLQTQNTHDGIKEQSAVDEEMPLFLKNSDCQTVEVISFECATSTENVSFVGDVETSEGSSQTAMQVSETEVQTMQQEFVVATMQTDEPLLVSDESQTEQTKSSEIDSQTDPPSTKSFICQTELITSSEVEVQAVRAVEFSLVECQTDALNHEEVEVQTELDISVSQSQTDNVDVGHIEMQTDMTITNASESQTDVLSVGDTEMQTDEKEIFTIESQTEQPSTELQETQTDDVSFAGDVPTTDQITQSESAQVFDSETQYALSIEEKDVQATPISFETQTQHSIELEVKSCQTEGVRMKNKKSQVFWNANSIDLPIIFIRVIPYFQKICLGRRRLNAVSSQNR

Target ORF information:

RefSeq Version	NM_182339.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TWiK family of potassium channels (twk-11), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182339.2

ORF Insert Sequence:

ATGTCCAATG ATAACTTGGC GATTGAAATC GACCCTCCGG ACGATGTGGA GTATGATGTG 
GAAGCAGGCT TCAACTCTTC CACATGCCAT TTAATGCACA ATTTCGAAGA GGAACGTCCA 
ACCTCTCTCG TAGCCTCCAT AAAGGAAAGT GCCATTAGCT CAAAGCGAAA ACTAAAATAC 
TACTGCTCGT TCATATGCAC CCCAATCAAG AAGTTGTTAT CCACTTTCAA ACTACTCATC 
ATAATCGGTC TTTACTCATT TATTGGCGCT CATATATTCA TGTACTTGGA AGTTCCAACT 
GATTTAGAAG CAAGAGAAGA TGGATTTCAT CAAAGAAAAA TAGCGAGGGA AGTGATGGTG 
CTGAATCTCC GTGCAATTTT CTACGACAAC CGCGAAGATC GCGAGGAACG ATGGAAGCAT 
GCCATTTTGA AGTTCGAAGA GGACATTGGC TTAGAAGAAC CTGTCATTGA AACTGTATGG 
ACTTTTTGGA TGTCTTTCCT CTATGCAGGA ACTATTTTCA CAACAATAGG ATATGGAAAC 
ATTGCATGTA AGACGCGTGC TGGCCAGATA GCTACAATGG TATATGCTTT TGTCGGAATT 
CCGATCATGC TGGTGATGCT CACCTCGCTC AACAATTTTT TGTTAAAATG GATAAAACTT 
ATTACGAATG GAGTTAGTGA TATGACGCTT TATATTGGTG TTCGCTTGGG AATAACAGTT 
ATTCGGCAGG ACGAAGTCCA GAAGCGCCTT CGCTACACAA AACTTGCCAA GACAATGAAA 
AGGTGGAAAC TTTCAAAACA CGGAGCTCCA AGTAGTATAG CGATTAGCAA TTCGGAAGAA 
AACCGGTTAA ATTCAACACC TGAAGACGAC GAAGAAGAGG AAGAAATTCA TCAGGATCCT 
CCAGTTCTTT CGACTCTAAT TGCAACTGTT GCCTGGATTA TTCTTTCGGC TGCTGTGTTT 
TGTCTTTTTG AGGACTGGAC ATTTTTCACC AGTTTTTATT TTTGTTTCAT CTCTCTGACA 
ACAATCGGTC TTGGAGATGT GACACCTGCT AATCCAGAAT ATATGATTGC AACATTTGGA 
GTTGTGATTG TCGGTTTATC AATGCTCACT GTCTGTATCG ACGTCTTACA AGAAAAACTT 
GCTCAAATGT ATATGGCTCT ACTTCAGAAG TTATTGACAG AGTACATGGA AGCTGTGAAA 
AGCGGAGACC CAAATGCTGC ATCAGCTATG ATGGCTGGTT TTCAAGGAAG GGCAAAGTTT 
TTGATGCCGT TGATTAGTAA AAACGAAGGA GCAAAAGTTA TGGATAAGTT CAAACAAGAT 
TGTTCAAAGA AGGGAATCGA TCCACCACCG GTTTTAACAA ATATCAACCC GGAGACCGGA 
ATGCCAGCTT TTGCAAATGC TCCGAAAGAA GATTTTAGAG ATTATATTGA AGTTGCAGAT 
GAAAAATTTG CTGATGAACA ACAGAGGCTC TCTCAAACCC CGTTATCAAA ATCCACCGAC 
TTTTTACAAT CACCCACACC ACGTCCAACT GTCACTGTAG CTGTTACTCA AACAGCTAGA 
CCACAGGCAT GCGTATCACT AGAAGTACAG GCTGGAACCA CAATGGGAAA GTTCCATGAT 
TATGGATCAC AGACACAGAC GGAAAGTTCT GACGAAGGAA TTCAGACGGA TTATAGCATT 
GATTTTGATG ATGAAAAAAA CAAGGAGCCA GAGAAAGTTA TGGCGGACAT TGGCACTCAA 
TATGAAATCG TTCTATTAAC AAGTATTGGA ATTCAACCAG AAGTCTCATC AATTTATATT 
GGAGCCGGCT TATCTGATCA TTCGGACGAA GAAAGTGGAT GTGAATCAGA AGACGATTCG 
GATGACTCGG ATGTATCATC CGATGAGGAA AGTGAACTTA AATCTTCTGC GAAATACAAA 
GAAACACCTC TTCCATTTGA AGTCGAAGAT CCAGGAATGA TGTCGCTCGG ATTACAGTCT 
CCACCAATTG AAATAACTCC CTTCACGGAT GATGAATCAG CTGCAAGCGA CGCAAGAAGT 
TCTCCTGTCC CACCAACATC TTCCAAGCGA TCCAAGTTGA CACCAAAGAC TATAAAAGTT 
GGCAAATCGT TATCAATGGA CTCGACTGAT ACAGTTATTC ATGTGAAACC AGTTGGCGTT 
AATGATGATG AGAAAAGAAG TGAAGAAAAA GAAAGTAAAG ATCAAGAAAC AAATGATGAC 
CTAAAAACTG AAAGTGGACA GGAGAAAAAG AGAAGAAAGA AAAAGAAGAA GCTTCGTTCA 
AAGAAGACAA GAGAGCATGA GCTGAGATTT TTGAAGAATA GCGGAAATCA AGCATCTCCC 
CTTCTTATGG ATTGCTCTAC ACAATATGAG ATATTTACAG TGGAAAGTGG GTCGCAATAC 
GATGTGAAAG GAAGAAGCCG AAAATGTCAG ACAAAACTTT CCAGTATAAA TCATGAGCAG 
TTACAAGGGA TTCGAGAAAC AGTTCAAAAG TTGAGAAGAA GAGAAAGTGA AATGAGTAAG 
GTGTCGAATG TTTCTGAAAG TGACTGGGAT AGTGAAACTG GAAAAGAACC GGAAGTCGCT 
GAAATCAGTT TAGATCTTTC TGTAGTTGCT CTCGGAGTTG ATGAATCCGG GAAAGTTGTT 
GATATTCCTA AGGAAGTACC TAAAGCTGTT CAACGACATG AATCATTTTC ACCAACATCG 
GAGCTCACCA CTGCGTCCGA ATCACTTTCC GCTGAGAAAG TACATCTGGA TTCAGAAGAT 
GAGGAGTTAG AAAGTCTTGA AGAACTCGAA CTGTATACCA CAGACGCCGG AATTCAGACT 
GAAACAAAGG TCATGCTGGA CAAGCAAGAT AATGCAACTC CAAAGTTGCT GGAAATTATT 
AAAAACTGCA GCACTCAGAC AACATTCAGC ACATTCCACA TTCCGCAGAA GGTATATGAG 
TCGGCGAGCA CCTCAAGTGA CGAGGAAGAT CATAAAAACG ATGGTGAAGA TGAAAAACAG 
AAGAAATCTA AACAGGAAGT GTTCTTTATG ACCAACACTG GAGTACAGAC GATGCATACA 
TCGTTCCATA TTGATGAAAA ACCGAAATTT GTTGAAGAAA ATCTTCAAAC TCAAAATACC 
CACGACGGCA TAAAAGAACA AAGTGCCGTT GATGAAGAAA TGCCTCTGTT CTTAAAAAAT 
TCGGATTGTC AAACCGTTGA AGTAATATCA TTCGAATGTG CCACATCAAC AGAGAATGTT 
AGTTTTGTTG GAGACGTTGA AACTTCTGAA GGATCCAGTC AGACAGCCAT GCAAGTTTCT 
GAAACTGAAG TACAAACAAT GCAACAAGAA TTTGTGGTAG CAACAATGCA AACCGACGAG 
CCACTGTTGG TATCTGATGA GTCACAGACT GAGCAAACAA AATCTTCGGA AATTGATTCT 
CAAACTGATC CACCATCCAC AAAATCATTT ATATGTCAGA CAGAATTGAT AACAAGCTCG 
GAAGTTGAAG TTCAAGCAGT TCGAGCTGTA GAGTTTTCAC TTGTCGAATG CCAAACAGAT 
GCGCTAAATC ATGAAGAAGT AGAAGTTCAA ACAGAACTGG ATATTTCGGT TTCGCAGTCC 
CAAACTGATA ATGTTGATGT AGGCCACATT GAAATGCAAA CTGATATGAC AATAACAAAT 
GCATCGGAAT CTCAAACCGA TGTGCTCAGT GTAGGGGACA CGGAGATGCA AACAGACGAG 
AAAGAAATAT TCACAATAGA ATCGCAAACC GAACAACCAT CCACAGAACT GCAAGAGACT 
CAAACTGACG ATGTTTCTTT TGCTGGCGAT GTTCCAACAA CCGACCAAAT CACACAATCG 
GAATCTGCTC AAGTTTTCGA TTCTGAAACT CAATATGCTT TATCCATAGA AGAAAAGGAC 
GTCCAGGCAA CGCCAATCTC ATTTGAAACT CAGACACAAC ATTCCATTGA ACTTGAAGTG 
AAATCTTGCC AAACCGAAGG AGTTCGAATG AAAAACAAAA AGAGTCAGGT ATTCTGGAAT 
GCTAATTCAA TTGATTTACC AATAATCTTT ATTCGTGTGA TTCCCTATTT CCAAAAAATT 
TGTTTAGGTA GAAGACGACT CAATGCTGTC AGTTCTCAAA ATAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twk-11 ( NM_182339.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_872139.2 Caenorhabditis elegans TWiK family of potassium channels (twk-11), mRNA.

Price & Availability↑

CloneID	OCa347228
Clone ID Related Accession (Same CDS sequence)	NM_182339.2 , NM_182339.3
Accession Version	NM_182339.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	TWiK family of potassium channels
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_182339.2.

ATGTCCAATG ATAACTTGGC GATTGAAATC GACCCTCCGG ACGATGTGGA GTATGATGTG 
GAAGCAGGCT TCAACTCTTC CACATGCCAT TTAATGCACA ATTTCGAAGA GGAACGTCCA 
ACCTCTCTCG TAGCCTCCAT AAAGGAAAGT GCCATTAGCT CAAAGCGAAA ACTAAAATAC 
TACTGCTCGT TCATATGCAC CCCAATCAAG AAGTTGTTAT CCACTTTCAA ACTACTCATC 
ATAATCGGTC TTTACTCATT TATTGGCGCT CATATATTCA TGTACTTGGA AGTTCCAACT 
GATTTAGAAG CAAGAGAAGA TGGATTTCAT CAAAGAAAAA TAGCGAGGGA AGTGATGGTG 
CTGAATCTCC GTGCAATTTT CTACGACAAC CGCGAAGATC GCGAGGAACG ATGGAAGCAT 
GCCATTTTGA AGTTCGAAGA GGACATTGGC TTAGAAGAAC CTGTCATTGA AACTGTATGG 
ACTTTTTGGA TGTCTTTCCT CTATGCAGGA ACTATTTTCA CAACAATAGG ATATGGAAAC 
ATTGCATGTA AGACGCGTGC TGGCCAGATA GCTACAATGG TATATGCTTT TGTCGGAATT 
CCGATCATGC TGGTGATGCT CACCTCGCTC AACAATTTTT TGTTAAAATG GATAAAACTT 
ATTACGAATG GAGTTAGTGA TATGACGCTT TATATTGGTG TTCGCTTGGG AATAACAGTT 
ATTCGGCAGG ACGAAGTCCA GAAGCGCCTT CGCTACACAA AACTTGCCAA GACAATGAAA 
AGGTGGAAAC TTTCAAAACA CGGAGCTCCA AGTAGTATAG CGATTAGCAA TTCGGAAGAA 
AACCGGTTAA ATTCAACACC TGAAGACGAC GAAGAAGAGG AAGAAATTCA TCAGGATCCT 
CCAGTTCTTT CGACTCTAAT TGCAACTGTT GCCTGGATTA TTCTTTCGGC TGCTGTGTTT 
TGTCTTTTTG AGGACTGGAC ATTTTTCACC AGTTTTTATT TTTGTTTCAT CTCTCTGACA 
ACAATCGGTC TTGGAGATGT GACACCTGCT AATCCAGAAT ATATGATTGC AACATTTGGA 
GTTGTGATTG TCGGTTTATC AATGCTCACT GTCTGTATCG ACGTCTTACA AGAAAAACTT 
GCTCAAATGT ATATGGCTCT ACTTCAGAAG TTATTGACAG AGTACATGGA AGCTGTGAAA 
AGCGGAGACC CAAATGCTGC ATCAGCTATG ATGGCTGGTT TTCAAGGAAG GGCAAAGTTT 
TTGATGCCGT TGATTAGTAA AAACGAAGGA GCAAAAGTTA TGGATAAGTT CAAACAAGAT 
TGTTCAAAGA AGGGAATCGA TCCACCACCG GTTTTAACAA ATATCAACCC GGAGACCGGA 
ATGCCAGCTT TTGCAAATGC TCCGAAAGAA GATTTTAGAG ATTATATTGA AGTTGCAGAT 
GAAAAATTTG CTGATGAACA ACAGAGGCTC TCTCAAACCC CGTTATCAAA ATCCACCGAC 
TTTTTACAAT CACCCACACC ACGTCCAACT GTCACTGTAG CTGTTACTCA AACAGCTAGA 
CCACAGGCAT GCGTATCACT AGAAGTACAG GCTGGAACCA CAATGGGAAA GTTCCATGAT 
TATGGATCAC AGACACAGAC GGAAAGTTCT GACGAAGGAA TTCAGACGGA TTATAGCATT 
GATTTTGATG ATGAAAAAAA CAAGGAGCCA GAGAAAGTTA TGGCGGACAT TGGCACTCAA 
TATGAAATCG TTCTATTAAC AAGTATTGGA ATTCAACCAG AAGTCTCATC AATTTATATT 
GGAGCCGGCT TATCTGATCA TTCGGACGAA GAAAGTGGAT GTGAATCAGA AGACGATTCG 
GATGACTCGG ATGTATCATC CGATGAGGAA AGTGAACTTA AATCTTCTGC GAAATACAAA 
GAAACACCTC TTCCATTTGA AGTCGAAGAT CCAGGAATGA TGTCGCTCGG ATTACAGTCT 
CCACCAATTG AAATAACTCC CTTCACGGAT GATGAATCAG CTGCAAGCGA CGCAAGAAGT 
TCTCCTGTCC CACCAACATC TTCCAAGCGA TCCAAGTTGA CACCAAAGAC TATAAAAGTT 
GGCAAATCGT TATCAATGGA CTCGACTGAT ACAGTTATTC ATGTGAAACC AGTTGGCGTT 
AATGATGATG AGAAAAGAAG TGAAGAAAAA GAAAGTAAAG ATCAAGAAAC AAATGATGAC 
CTAAAAACTG AAAGTGGACA GGAGAAAAAG AGAAGAAAGA AAAAGAAGAA GCTTCGTTCA 
AAGAAGACAA GAGAGCATGA GCTGAGATTT TTGAAGAATA GCGGAAATCA AGCATCTCCC 
CTTCTTATGG ATTGCTCTAC ACAATATGAG ATATTTACAG TGGAAAGTGG GTCGCAATAC 
GATGTGAAAG GAAGAAGCCG AAAATGTCAG ACAAAACTTT CCAGTATAAA TCATGAGCAG 
TTACAAGGGA TTCGAGAAAC AGTTCAAAAG TTGAGAAGAA GAGAAAGTGA AATGAGTAAG 
GTGTCGAATG TTTCTGAAAG TGACTGGGAT AGTGAAACTG GAAAAGAACC GGAAGTCGCT 
GAAATCAGTT TAGATCTTTC TGTAGTTGCT CTCGGAGTTG ATGAATCCGG GAAAGTTGTT 
GATATTCCTA AGGAAGTACC TAAAGCTGTT CAACGACATG AATCATTTTC ACCAACATCG 
GAGCTCACCA CTGCGTCCGA ATCACTTTCC GCTGAGAAAG TACATCTGGA TTCAGAAGAT 
GAGGAGTTAG AAAGTCTTGA AGAACTCGAA CTGTATACCA CAGACGCCGG AATTCAGACT 
GAAACAAAGG TCATGCTGGA CAAGCAAGAT AATGCAACTC CAAAGTTGCT GGAAATTATT 
AAAAACTGCA GCACTCAGAC AACATTCAGC ACATTCCACA TTCCGCAGAA GGTATATGAG 
TCGGCGAGCA CCTCAAGTGA CGAGGAAGAT CATAAAAACG ATGGTGAAGA TGAAAAACAG 
AAGAAATCTA AACAGGAAGT GTTCTTTATG ACCAACACTG GAGTACAGAC GATGCATACA 
TCGTTCCATA TTGATGAAAA ACCGAAATTT GTTGAAGAAA ATCTTCAAAC TCAAAATACC 
CACGACGGCA TAAAAGAACA AAGTGCCGTT GATGAAGAAA TGCCTCTGTT CTTAAAAAAT 
TCGGATTGTC AAACCGTTGA AGTAATATCA TTCGAATGTG CCACATCAAC AGAGAATGTT 
AGTTTTGTTG GAGACGTTGA AACTTCTGAA GGATCCAGTC AGACAGCCAT GCAAGTTTCT 
GAAACTGAAG TACAAACAAT GCAACAAGAA TTTGTGGTAG CAACAATGCA AACCGACGAG 
CCACTGTTGG TATCTGATGA GTCACAGACT GAGCAAACAA AATCTTCGGA AATTGATTCT 
CAAACTGATC CACCATCCAC AAAATCATTT ATATGTCAGA CAGAATTGAT AACAAGCTCG 
GAAGTTGAAG TTCAAGCAGT TCGAGCTGTA GAGTTTTCAC TTGTCGAATG CCAAACAGAT 
GCGCTAAATC ATGAAGAAGT AGAAGTTCAA ACAGAACTGG ATATTTCGGT TTCGCAGTCC 
CAAACTGATA ATGTTGATGT AGGCCACATT GAAATGCAAA CTGATATGAC AATAACAAAT 
GCATCGGAAT CTCAAACCGA TGTGCTCAGT GTAGGGGACA CGGAGATGCA AACAGACGAG 
AAAGAAATAT TCACAATAGA ATCGCAAACC GAACAACCAT CCACAGAACT GCAAGAGACT 
CAAACTGACG ATGTTTCTTT TGCTGGCGAT GTTCCAACAA CCGACCAAAT CACACAATCG 
GAATCTGCTC AAGTTTTCGA TTCTGAAACT CAATATGCTT TATCCATAGA AGAAAAGGAC 
GTCCAGGCAA CGCCAATCTC ATTTGAAACT CAGACACAAC ATTCCATTGA ACTTGAAGTG 
AAATCTTGCC AAACCGAAGG AGTTCGAATG AAAAACAAAA AGAGTCAGGT ATTCTGGAAT 
GCTAATTCAA TTGATTTACC AATAATCTTT ATTCGTGTGA TTCCCTATTT CCAAAAAATT 
TGTTTAGGTA GAAGACGACT CAATGCTGTC AGTTCTCAAA ATAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_872139.2
CDS	129..4256
Translation	MSNDNLAIEIDPPDDVEYDVEAGFNSSTCHLMHNFEEERPTSLVASIKESAISSKRKLKYYCSFICTPIKKLLSTFKLLIIIGLYSFIGAHIFMYLEVPTDLEAREDGFHQRKIAREVMVLNLRAIFYDNREDREERWKHAILKFEEDIGLEEPVIETVWTFWMSFLYAGTIFTTIGYGNIACKTRAGQIATMVYAFVGIPIMLVMLTSLNNFLLKWIKLITNGVSDMTLYIGVRLGITVIRQDEVQKRLRYTKLAKTMKRWKLSKHGAPSSIAISNSEENRLNSTPEDDEEEEEIHQDPPVLSTLIATVAWIILSAAVFCLFEDWTFFTSFYFCFISLTTIGLGDVTPANPEYMIATFGVVIVGLSMLTVCIDVLQEKLAQMYMALLQKLLTEYMEAVKSGDPNAASAMMAGFQGRAKFLMPLISKNEGAKVMDKFKQDCSKKGIDPPPVLTNINPETGMPAFANAPKEDFRDYIEVADEKFADEQQRLSQTPLSKSTDFLQSPTPRPTVTVAVTQTARPQACVSLEVQAGTTMGKFHDYGSQTQTESSDEGIQTDYSIDFDDEKNKEPEKVMADIGTQYEIVLLTSIGIQPEVSSIYIGAGLSDHSDEESGCESEDDSDDSDVSSDEESELKSSAKYKETPLPFEVEDPGMMSLGLQSPPIEITPFTDDESAASDARSSPVPPTSSKRSKLTPKTIKVGKSLSMDSTDTVIHVKPVGVNDDEKRSEEKESKDQETNDDLKTESGQEKKRRKKKKKLRSKKTREHELRFLKNSGNQASPLLMDCSTQYEIFTVESGSQYDVKGRSRKCQTKLSSINHEQLQGIRETVQKLRRRESEMSKVSNVSESDWDSETGKEPEVAEISLDLSVVALGVDESGKVVDIPKEVPKAVQRHESFSPTSELTTASESLSAEKVHLDSEDEELESLEELELYTTDAGIQTETKVMLDKQDNATPKLLEIIKNCSTQTTFSTFHIPQKVYESASTSSDEEDHKNDGEDEKQKKSKQEVFFMTNTGVQTMHTSFHIDEKPKFVEENLQTQNTHDGIKEQSAVDEEMPLFLKNSDCQTVEVISFECATSTENVSFVGDVETSEGSSQTAMQVSETEVQTMQQEFVVATMQTDEPLLVSDESQTEQTKSSEIDSQTDPPSTKSFICQTELITSSEVEVQAVRAVEFSLVECQTDALNHEEVEVQTELDISVSQSQTDNVDVGHIEMQTDMTITNASESQTDVLSVGDTEMQTDEKEIFTIESQTEQPSTELQETQTDDVSFAGDVPTTDQITQSESAQVFDSETQYALSIEEKDVQATPISFETQTQHSIELEVKSCQTEGVRMKNKKSQVFWNANSIDLPIIFIRVIPYFQKICLGRRRLNAVSSQNR

Target ORF information:

RefSeq Version	NM_182339.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TWiK family of potassium channels (twk-11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182339.3

ORF Insert Sequence:

ATGTCCAATG ATAACTTGGC GATTGAAATC GACCCTCCGG ACGATGTGGA GTATGATGTG 
GAAGCAGGCT TCAACTCTTC CACATGCCAT TTAATGCACA ATTTCGAAGA GGAACGTCCA 
ACCTCTCTCG TAGCCTCCAT AAAGGAAAGT GCCATTAGCT CAAAGCGAAA ACTAAAATAC 
TACTGCTCGT TCATATGCAC CCCAATCAAG AAGTTGTTAT CCACTTTCAA ACTACTCATC 
ATAATCGGTC TTTACTCATT TATTGGCGCT CATATATTCA TGTACTTGGA AGTTCCAACT 
GATTTAGAAG CAAGAGAAGA TGGATTTCAT CAAAGAAAAA TAGCGAGGGA AGTGATGGTG 
CTGAATCTCC GTGCAATTTT CTACGACAAC CGCGAAGATC GCGAGGAACG ATGGAAGCAT 
GCCATTTTGA AGTTCGAAGA GGACATTGGC TTAGAAGAAC CTGTCATTGA AACTGTATGG 
ACTTTTTGGA TGTCTTTCCT CTATGCAGGA ACTATTTTCA CAACAATAGG ATATGGAAAC 
ATTGCATGTA AGACGCGTGC TGGCCAGATA GCTACAATGG TATATGCTTT TGTCGGAATT 
CCGATCATGC TGGTGATGCT CACCTCGCTC AACAATTTTT TGTTAAAATG GATAAAACTT 
ATTACGAATG GAGTTAGTGA TATGACGCTT TATATTGGTG TTCGCTTGGG AATAACAGTT 
ATTCGGCAGG ACGAAGTCCA GAAGCGCCTT CGCTACACAA AACTTGCCAA GACAATGAAA 
AGGTGGAAAC TTTCAAAACA CGGAGCTCCA AGTAGTATAG CGATTAGCAA TTCGGAAGAA 
AACCGGTTAA ATTCAACACC TGAAGACGAC GAAGAAGAGG AAGAAATTCA TCAGGATCCT 
CCAGTTCTTT CGACTCTAAT TGCAACTGTT GCCTGGATTA TTCTTTCGGC TGCTGTGTTT 
TGTCTTTTTG AGGACTGGAC ATTTTTCACC AGTTTTTATT TTTGTTTCAT CTCTCTGACA 
ACAATCGGTC TTGGAGATGT GACACCTGCT AATCCAGAAT ATATGATTGC AACATTTGGA 
GTTGTGATTG TCGGTTTATC AATGCTCACT GTCTGTATCG ACGTCTTACA AGAAAAACTT 
GCTCAAATGT ATATGGCTCT ACTTCAGAAG TTATTGACAG AGTACATGGA AGCTGTGAAA 
AGCGGAGACC CAAATGCTGC ATCAGCTATG ATGGCTGGTT TTCAAGGAAG GGCAAAGTTT 
TTGATGCCGT TGATTAGTAA AAACGAAGGA GCAAAAGTTA TGGATAAGTT CAAACAAGAT 
TGTTCAAAGA AGGGAATCGA TCCACCACCG GTTTTAACAA ATATCAACCC GGAGACCGGA 
ATGCCAGCTT TTGCAAATGC TCCGAAAGAA GATTTTAGAG ATTATATTGA AGTTGCAGAT 
GAAAAATTTG CTGATGAACA ACAGAGGCTC TCTCAAACCC CGTTATCAAA ATCCACCGAC 
TTTTTACAAT CACCCACACC ACGTCCAACT GTCACTGTAG CTGTTACTCA AACAGCTAGA 
CCACAGGCAT GCGTATCACT AGAAGTACAG GCTGGAACCA CAATGGGAAA GTTCCATGAT 
TATGGATCAC AGACACAGAC GGAAAGTTCT GACGAAGGAA TTCAGACGGA TTATAGCATT 
GATTTTGATG ATGAAAAAAA CAAGGAGCCA GAGAAAGTTA TGGCGGACAT TGGCACTCAA 
TATGAAATCG TTCTATTAAC AAGTATTGGA ATTCAACCAG AAGTCTCATC AATTTATATT 
GGAGCCGGCT TATCTGATCA TTCGGACGAA GAAAGTGGAT GTGAATCAGA AGACGATTCG 
GATGACTCGG ATGTATCATC CGATGAGGAA AGTGAACTTA AATCTTCTGC GAAATACAAA 
GAAACACCTC TTCCATTTGA AGTCGAAGAT CCAGGAATGA TGTCGCTCGG ATTACAGTCT 
CCACCAATTG AAATAACTCC CTTCACGGAT GATGAATCAG CTGCAAGCGA CGCAAGAAGT 
TCTCCTGTCC CACCAACATC TTCCAAGCGA TCCAAGTTGA CACCAAAGAC TATAAAAGTT 
GGCAAATCGT TATCAATGGA CTCGACTGAT ACAGTTATTC ATGTGAAACC AGTTGGCGTT 
AATGATGATG AGAAAAGAAG TGAAGAAAAA GAAAGTAAAG ATCAAGAAAC AAATGATGAC 
CTAAAAACTG AAAGTGGACA GGAGAAAAAG AGAAGAAAGA AAAAGAAGAA GCTTCGTTCA 
AAGAAGACAA GAGAGCATGA GCTGAGATTT TTGAAGAATA GCGGAAATCA AGCATCTCCC 
CTTCTTATGG ATTGCTCTAC ACAATATGAG ATATTTACAG TGGAAAGTGG GTCGCAATAC 
GATGTGAAAG GAAGAAGCCG AAAATGTCAG ACAAAACTTT CCAGTATAAA TCATGAGCAG 
TTACAAGGGA TTCGAGAAAC AGTTCAAAAG TTGAGAAGAA GAGAAAGTGA AATGAGTAAG 
GTGTCGAATG TTTCTGAAAG TGACTGGGAT AGTGAAACTG GAAAAGAACC GGAAGTCGCT 
GAAATCAGTT TAGATCTTTC TGTAGTTGCT CTCGGAGTTG ATGAATCCGG GAAAGTTGTT 
GATATTCCTA AGGAAGTACC TAAAGCTGTT CAACGACATG AATCATTTTC ACCAACATCG 
GAGCTCACCA CTGCGTCCGA ATCACTTTCC GCTGAGAAAG TACATCTGGA TTCAGAAGAT 
GAGGAGTTAG AAAGTCTTGA AGAACTCGAA CTGTATACCA CAGACGCCGG AATTCAGACT 
GAAACAAAGG TCATGCTGGA CAAGCAAGAT AATGCAACTC CAAAGTTGCT GGAAATTATT 
AAAAACTGCA GCACTCAGAC AACATTCAGC ACATTCCACA TTCCGCAGAA GGTATATGAG 
TCGGCGAGCA CCTCAAGTGA CGAGGAAGAT CATAAAAACG ATGGTGAAGA TGAAAAACAG 
AAGAAATCTA AACAGGAAGT GTTCTTTATG ACCAACACTG GAGTACAGAC GATGCATACA 
TCGTTCCATA TTGATGAAAA ACCGAAATTT GTTGAAGAAA ATCTTCAAAC TCAAAATACC 
CACGACGGCA TAAAAGAACA AAGTGCCGTT GATGAAGAAA TGCCTCTGTT CTTAAAAAAT 
TCGGATTGTC AAACCGTTGA AGTAATATCA TTCGAATGTG CCACATCAAC AGAGAATGTT 
AGTTTTGTTG GAGACGTTGA AACTTCTGAA GGATCCAGTC AGACAGCCAT GCAAGTTTCT 
GAAACTGAAG TACAAACAAT GCAACAAGAA TTTGTGGTAG CAACAATGCA AACCGACGAG 
CCACTGTTGG TATCTGATGA GTCACAGACT GAGCAAACAA AATCTTCGGA AATTGATTCT 
CAAACTGATC CACCATCCAC AAAATCATTT ATATGTCAGA CAGAATTGAT AACAAGCTCG 
GAAGTTGAAG TTCAAGCAGT TCGAGCTGTA GAGTTTTCAC TTGTCGAATG CCAAACAGAT 
GCGCTAAATC ATGAAGAAGT AGAAGTTCAA ACAGAACTGG ATATTTCGGT TTCGCAGTCC 
CAAACTGATA ATGTTGATGT AGGCCACATT GAAATGCAAA CTGATATGAC AATAACAAAT 
GCATCGGAAT CTCAAACCGA TGTGCTCAGT GTAGGGGACA CGGAGATGCA AACAGACGAG 
AAAGAAATAT TCACAATAGA ATCGCAAACC GAACAACCAT CCACAGAACT GCAAGAGACT 
CAAACTGACG ATGTTTCTTT TGCTGGCGAT GTTCCAACAA CCGACCAAAT CACACAATCG 
GAATCTGCTC AAGTTTTCGA TTCTGAAACT CAATATGCTT TATCCATAGA AGAAAAGGAC 
GTCCAGGCAA CGCCAATCTC ATTTGAAACT CAGACACAAC ATTCCATTGA ACTTGAAGTG 
AAATCTTGCC AAACCGAAGG AGTTCGAATG AAAAACAAAA AGAGTCAGGT ATTCTGGAAT 
GCTAATTCAA TTGATTTACC AATAATCTTT ATTCGTGTGA TTCCCTATTT CCAAAAAATT 
TGTTTAGGTA GAAGACGACT CAATGCTGTC AGTTCTCAAA ATAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twk-11 ( NM_001356689.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001343632.1 Caenorhabditis elegans TWiK family of potassium channels (twk-11), partial mRNA.

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CloneID	OCa529871
Clone ID Related Accession (Same CDS sequence)	NM_001356689.1 , NM_001356689.2
Accession Version	NM_001356689.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	TWiK family of potassium channels
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGTCCAATG ATAACTTGGC GATTGAAATC GACCCTCCGG ACGATGTGGA GTATGATGTG 
GAAGCAGGCT TCAACTCTTC CACATGCCAT TTAATGCACA ATTTCGAAGA GGAACGTCCA 
ACCTCTCTCG TAGCCTCCAT AAAGGAAAGT GCCATTAGCT CAAAGCGAAA ACTAAAATAC 
TACTGCTCGT TCATATGCAC CCCAATCAAG AAGTTGTTAT CCACTTTCAA ACTACTCATC 
ATAATCGGTC TTTACTCATT TATTGGCGCT CATATATTCA TGTACTTGGA AGTTCCAACT 
GATTTAGAAG CAAGAGAAGA TGGATTTCAT CAAAGAAAAA TAGCGAGGGA AGTGATGGTG 
CTGAATCTCC GTGCAATTTT CTACGACAAC CGCGAAGATC GCGAGGAACG ATGGAAGCAT 
GCCATTTTGA AGTTCGAAGA GGACATTGGC TTAGAAGAAC CTGTCATTGA AACTGTATGG 
ACTTTTTGGA TGTCTTTCCT CTATGCAGGA ACTATTTTCA CAACAATAGG ATATGGAAAC 
ATTGCATGTA AGACGCGTGC TGGCCAGATA GCTACAATGG TATATGCTTT TGTCGGAATT 
CCGATCATGC TGGTGATGCT CACCTCGCTC AACAATTTTT TGTTAAAATG GATAAAACTT 
ATTACGAATG GAGTTAGTGA TATGACGCTT TATATTGGTG TTCGCTTGGG AATAACAGTT 
ATTCGGCAGG ACGAAGTCCA GAAGCGCCTT CGCTACACAA AACTTGCCAA GACAATGAAA 
AGGTGGAAAC TTTCAAAACA CGGAGCTCCA AGTAGTATAG CGATTAGCAA TTCGGAAGAA 
AACCGGTTAA ATTCAACACC TGAAGACGAC GAAGAAGAGG AAGAAATTCA TCAGGATCCT 
CCAGTTCTTT CGACTCTAAT TGCAACTGTT GCCTGGATTA TTCTTTCGGC TGCTGTGTTT 
TGTCTTTTTG AGGACTGGAC ATTTTTCACC AGTTTTTATT TTTGTTTCAT CTCTCTGACA 
ACAATCGGTC TTGGAGATGT GACACCTGCT AATCCAGAAT ATATGATTGC AACATTTGGA 
GTTGTGATTG TCGGTTTATC AATGCTCACT GTCTGTATCG ACGTCTTACA AGAAAAACTT 
GCTCAAATGT ATATGGCTCT ACTTCAGAAG TTATTGACAG AGTACATGGA AGCTGTGAAA 
AGCGGAGACC CAAATGCTGC ATCAGCTATG ATGGCTGGTT TTCAAGGAAG GGCAAAGTTT 
TTGATGCCGT TGATTAGTAA AAACGAAGGA GCAAAAGTTA TGGATAAGTT CAAACAAGAT 
TGTTCAAAGA AGGGAATCGA TCCACCACCG GTTTTAACAA ATATCAACCC GGAGACCGGA 
ATGCCAGCTT TTGCAAATGC TCCGAAAGAA GATTTTAGAG ATTATATTGA AGTTGCAGAT 
GAAAAATTTG CTGATGAACA ACAGAGGCTC TCTCAAACCC CGTTATCAAA ATCCACCGAC 
TTTTTACAAT CACCCACACC ACGTCCAACT GTCACTGTAG CTGTTACTCA AACAGCTAGA 
CCACAGGCAT GCGTATCACT AGAAGTACAG GCTGGAACCA CAATGGGAAA GTTCCATGAT 
TATGGATCAC AGACACAGAC GGAAAGTTCT GACGAAGGAA TTCAGACGGA TTATAGCATT 
GATTTTGATG ATGAAAAAAA CAAGGAGCCA GAGAAAGTTA TGGCGGACAT TGGCACTCAA 
TATGAAATCG TTCTATTAAC AAGTATTGGA ATTCAACCAG AAGTCTCATC AATTTATATT 
GGAGCCGGCT TATCTGATCA TTCGGACGAA GAAAGTGGAT GTGAATCAGA AGACGATTCG 
GATGACTCGG ATGTATCATC CGATGAGGAA AGTGAACTTA AATCTTCTGC GAAATACAAA 
GAAACACCTC TTCCATTTGA AGTCGAAGAT CCAGGAATGA TGTCGCTCGG ATTACAGTCT 
CCACCAATTG AAATAACTCC CTTCACGGAT GATGAATCAG CTGCAAGCGA CGCAAGAAGT 
TCTCCTGTCC CACCAACATC TTCCAAGCGA TCCAAGTTGA CACCAAAGAC TATAAAAGTT 
GGCAAATCGT TATCAATGGA CTCGACTGAT ACAGTTATTC ATGTGAAACC AGTTGGCGTT 
AATGATGATG AGAAAAGAAG TGAAGAAAAA GAAAGTAAAG ATCAAGAAAC AAATGATGAC 
CTAAAAACTG AAAGTGGACA GGAGAAAAAG AGAAGAAAGA AAAAGAAGAA GCTTCGTTCA 
AAGAAGACAA GAGAGCATGA GCTGAGATTT TTGAAGAATA GCGGAAATCA AGCATCTCCC 
CTTCTTATGG ATTGCTCTAC ACAATATGAG ATATTTACAG TGGAAAGTGG GTCGCAATAC 
GATGTGAAAG GAAGAAGCCG AAAATGTCAG ACAAAACTTT CCAGTATAAA TCATGAGCAG 
TTACAAGGGA TTCGAGAAAC AGTTCAAAAG TTGAGAAGAA GAGAAAGTGA AATGAGTAAG 
GTGTCGAATG TTTCTGAAAG TGACTGGGAT AGTGAAACTG GAAAAGAACC GGAAGTCGCT 
GAAATCAGTT TAGATCTTTC TGTAGTTGCT CTCGGAGTTG ATGAATCCGG GAAAGTTGTT 
GATATTCCTA AGGAAGTACC TAAAGCTGTT CAACGACATG AATCATTTTC ACCAACATCG 
GAGCTCACCA CTGCGTCCGA ATCACTTTCC GCTGAGAAAG TACATCTGGA TTCAGAAGAT 
GAGGAGTTAG AAAGTCTTGA AGAACTCGAA CTGTATACCA CAGACGCCGG AATTCAGACT 
GAAACAAAGG TCATGCTGGA CAAGCAAGAT AATGCAACTC CAAAGTTGCT GGAAATTATT 
AAAAACTGCA GCACTCAGAC AACATTCAGC ACATTCCACA TTCCGCAGAA GGTATATGAG 
TCGGCGAGCA CCTCAAGTGA CGAGGAAGAT CATAAAAACG ATGGTGAAGA TGAAAAACAG 
AAGAAATCTA AACAGGAAGT GTTCTTTATG ACCAACACTG GAGTACAGAC GATGCATACA 
TCGTTCCATA TTGATGAAAA ACCGAAATTT GTTGAAGAAA ATCTTCAAAC TCAAAATACC 
CACGACGGCA TAAAAGAACA AAGTGCCGTT GATGAAGAAA TGCCTCTGTT CTTAAAAAAT 
TCGGATTGTC AAACCGTTGA AGTAATATCA TTCGAATGTG CCACATCAAC AGAGAATGTT 
AGTTTTGTTG GAGACGTTGA AACTTCTGAA GGATCCAGTC AGACAGCCAT GCAAGTTTCT 
GAAACTGAAG TACAAACAAT GCAACAAGAA TTTGTGGTAG CAACAATGCA AACCGACGAG 
CCACTGTTGG TATCTGATGA GTCACAGACT GAGCAAACAA AATCTTCGGA AATTGATTCT 
CAAACTGATC CACCATCCAC AAAATCATTT ATATGTCAGA CAGAATTGAT AACAAGCTCG 
GAAGTTGAAG TTCAAGCAGT TCGAGCTGTA GAGTTTTCAC TTGTCGAATG CCAAACAGAT 
GCGCTAAATC ATGAAGAAGT AGAAGTTCAA ACAGAACTGG ATATTTCGGT TTCGCAGTCC 
CAAACTGATA ATGTTGATGT AGGCCACATT GAAATGCAAA CTGATATGAC AATAACAAAT 
GCATCGGAAT CTCAAACCGA TGTGCTCAGT GTAGGGGACA CGGAGATGCA AACAGACGAG 
AAAGAAATAT TCACAATAGA ATCGCAAACC GAACAACCAT CCACAGAACT GCAAGAGACT 
CAAACTGACG ATGTTTCTTT TGCTGGCGAT GTTCCAACAA CCGACCAAAT CACACAATCG 
GAATCTGCTC AAGTTTTCGA TTCTGAAACT CAATATGCTT TATCCATAGA AGAAAAGGAC 
GTCCAGGCAA CGCCAATCTC ATTTGAAACT CAGACACAAC ATTCCATTGA ACTTGAAGTG 
AAATCTTGCC AAACCGAAGG AGTTCGAATG AAAAACAAAA AGAGTCAGGT GTATGTTCAA 
GAACTCGATG AAGAACGTCG AATGCGTATG TCAGACATTG GCATACAAAC CGGTGTTCTG 
GCACGTGTTC AACACATTTA TACCCGTCCA ATATCTGATG ATGTGGGACC ATCTTCATCT 
GCTCCAATCA CTCTTCGAAA GTCTTCCGAC TCCTCGGAAC ACATATCAAC GAAAACTGCG 
AAAAGCAGCG AAGAGTCATT GGCGCCATCC GCTTCCGGGA CAGCGCTCTC TGCAGCTGAA 
TCAATGGATG ATCATTTATG GCCAGAGAGA GCAAGTCCAT TTGCATCGAG GCATGGCTCA 
TTGAAGAGAA GAACGAGGGA ACTTGATCCC GAGAAAAAGG GGGACAAAAC AAATATTGTT 
CAAGATCTAC GTGCAAGATT TGAAATGAAA TCTCCACCAA AATCACCACC GAAAGATGAT 
GAAGAAGGAA CATCTACGGA TCCCAAACAT TTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343632.1
CDS	1..4479
Translation	MSNDNLAIEIDPPDDVEYDVEAGFNSSTCHLMHNFEEERPTSLVASIKESAISSKRKLKYYCSFICTPIKKLLSTFKLLIIIGLYSFIGAHIFMYLEVPTDLEAREDGFHQRKIAREVMVLNLRAIFYDNREDREERWKHAILKFEEDIGLEEPVIETVWTFWMSFLYAGTIFTTIGYGNIACKTRAGQIATMVYAFVGIPIMLVMLTSLNNFLLKWIKLITNGVSDMTLYIGVRLGITVIRQDEVQKRLRYTKLAKTMKRWKLSKHGAPSSIAISNSEENRLNSTPEDDEEEEEIHQDPPVLSTLIATVAWIILSAAVFCLFEDWTFFTSFYFCFISLTTIGLGDVTPANPEYMIATFGVVIVGLSMLTVCIDVLQEKLAQMYMALLQKLLTEYMEAVKSGDPNAASAMMAGFQGRAKFLMPLISKNEGAKVMDKFKQDCSKKGIDPPPVLTNINPETGMPAFANAPKEDFRDYIEVADEKFADEQQRLSQTPLSKSTDFLQSPTPRPTVTVAVTQTARPQACVSLEVQAGTTMGKFHDYGSQTQTESSDEGIQTDYSIDFDDEKNKEPEKVMADIGTQYEIVLLTSIGIQPEVSSIYIGAGLSDHSDEESGCESEDDSDDSDVSSDEESELKSSAKYKETPLPFEVEDPGMMSLGLQSPPIEITPFTDDESAASDARSSPVPPTSSKRSKLTPKTIKVGKSLSMDSTDTVIHVKPVGVNDDEKRSEEKESKDQETNDDLKTESGQEKKRRKKKKKLRSKKTREHELRFLKNSGNQASPLLMDCSTQYEIFTVESGSQYDVKGRSRKCQTKLSSINHEQLQGIRETVQKLRRRESEMSKVSNVSESDWDSETGKEPEVAEISLDLSVVALGVDESGKVVDIPKEVPKAVQRHESFSPTSELTTASESLSAEKVHLDSEDEELESLEELELYTTDAGIQTETKVMLDKQDNATPKLLEIIKNCSTQTTFSTFHIPQKVYESASTSSDEEDHKNDGEDEKQKKSKQEVFFMTNTGVQTMHTSFHIDEKPKFVEENLQTQNTHDGIKEQSAVDEEMPLFLKNSDCQTVEVISFECATSTENVSFVGDVETSEGSSQTAMQVSETEVQTMQQEFVVATMQTDEPLLVSDESQTEQTKSSEIDSQTDPPSTKSFICQTELITSSEVEVQAVRAVEFSLVECQTDALNHEEVEVQTELDISVSQSQTDNVDVGHIEMQTDMTITNASESQTDVLSVGDTEMQTDEKEIFTIESQTEQPSTELQETQTDDVSFAGDVPTTDQITQSESAQVFDSETQYALSIEEKDVQATPISFETQTQHSIELEVKSCQTEGVRMKNKKSQVYVQELDEERRMRMSDIGIQTGVLARVQHIYTRPISDDVGPSSSAPITLRKSSDSSEHISTKTAKSSEESLAPSASGTALSAAESMDDHLWPERASPFASRHGSLKRRTRELDPEKKGDKTNIVQDLRARFEMKSPPKSPPKDDEEGTSTDPKHFF

Target ORF information:

RefSeq Version	NM_001356689.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TWiK family of potassium channels (twk-11), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356689.1

ORF Insert Sequence:

ATGTCCAATG ATAACTTGGC GATTGAAATC GACCCTCCGG ACGATGTGGA GTATGATGTG 
GAAGCAGGCT TCAACTCTTC CACATGCCAT TTAATGCACA ATTTCGAAGA GGAACGTCCA 
ACCTCTCTCG TAGCCTCCAT AAAGGAAAGT GCCATTAGCT CAAAGCGAAA ACTAAAATAC 
TACTGCTCGT TCATATGCAC CCCAATCAAG AAGTTGTTAT CCACTTTCAA ACTACTCATC 
ATAATCGGTC TTTACTCATT TATTGGCGCT CATATATTCA TGTACTTGGA AGTTCCAACT 
GATTTAGAAG CAAGAGAAGA TGGATTTCAT CAAAGAAAAA TAGCGAGGGA AGTGATGGTG 
CTGAATCTCC GTGCAATTTT CTACGACAAC CGCGAAGATC GCGAGGAACG ATGGAAGCAT 
GCCATTTTGA AGTTCGAAGA GGACATTGGC TTAGAAGAAC CTGTCATTGA AACTGTATGG 
ACTTTTTGGA TGTCTTTCCT CTATGCAGGA ACTATTTTCA CAACAATAGG ATATGGAAAC 
ATTGCATGTA AGACGCGTGC TGGCCAGATA GCTACAATGG TATATGCTTT TGTCGGAATT 
CCGATCATGC TGGTGATGCT CACCTCGCTC AACAATTTTT TGTTAAAATG GATAAAACTT 
ATTACGAATG GAGTTAGTGA TATGACGCTT TATATTGGTG TTCGCTTGGG AATAACAGTT 
ATTCGGCAGG ACGAAGTCCA GAAGCGCCTT CGCTACACAA AACTTGCCAA GACAATGAAA 
AGGTGGAAAC TTTCAAAACA CGGAGCTCCA AGTAGTATAG CGATTAGCAA TTCGGAAGAA 
AACCGGTTAA ATTCAACACC TGAAGACGAC GAAGAAGAGG AAGAAATTCA TCAGGATCCT 
CCAGTTCTTT CGACTCTAAT TGCAACTGTT GCCTGGATTA TTCTTTCGGC TGCTGTGTTT 
TGTCTTTTTG AGGACTGGAC ATTTTTCACC AGTTTTTATT TTTGTTTCAT CTCTCTGACA 
ACAATCGGTC TTGGAGATGT GACACCTGCT AATCCAGAAT ATATGATTGC AACATTTGGA 
GTTGTGATTG TCGGTTTATC AATGCTCACT GTCTGTATCG ACGTCTTACA AGAAAAACTT 
GCTCAAATGT ATATGGCTCT ACTTCAGAAG TTATTGACAG AGTACATGGA AGCTGTGAAA 
AGCGGAGACC CAAATGCTGC ATCAGCTATG ATGGCTGGTT TTCAAGGAAG GGCAAAGTTT 
TTGATGCCGT TGATTAGTAA AAACGAAGGA GCAAAAGTTA TGGATAAGTT CAAACAAGAT 
TGTTCAAAGA AGGGAATCGA TCCACCACCG GTTTTAACAA ATATCAACCC GGAGACCGGA 
ATGCCAGCTT TTGCAAATGC TCCGAAAGAA GATTTTAGAG ATTATATTGA AGTTGCAGAT 
GAAAAATTTG CTGATGAACA ACAGAGGCTC TCTCAAACCC CGTTATCAAA ATCCACCGAC 
TTTTTACAAT CACCCACACC ACGTCCAACT GTCACTGTAG CTGTTACTCA AACAGCTAGA 
CCACAGGCAT GCGTATCACT AGAAGTACAG GCTGGAACCA CAATGGGAAA GTTCCATGAT 
TATGGATCAC AGACACAGAC GGAAAGTTCT GACGAAGGAA TTCAGACGGA TTATAGCATT 
GATTTTGATG ATGAAAAAAA CAAGGAGCCA GAGAAAGTTA TGGCGGACAT TGGCACTCAA 
TATGAAATCG TTCTATTAAC AAGTATTGGA ATTCAACCAG AAGTCTCATC AATTTATATT 
GGAGCCGGCT TATCTGATCA TTCGGACGAA GAAAGTGGAT GTGAATCAGA AGACGATTCG 
GATGACTCGG ATGTATCATC CGATGAGGAA AGTGAACTTA AATCTTCTGC GAAATACAAA 
GAAACACCTC TTCCATTTGA AGTCGAAGAT CCAGGAATGA TGTCGCTCGG ATTACAGTCT 
CCACCAATTG AAATAACTCC CTTCACGGAT GATGAATCAG CTGCAAGCGA CGCAAGAAGT 
TCTCCTGTCC CACCAACATC TTCCAAGCGA TCCAAGTTGA CACCAAAGAC TATAAAAGTT 
GGCAAATCGT TATCAATGGA CTCGACTGAT ACAGTTATTC ATGTGAAACC AGTTGGCGTT 
AATGATGATG AGAAAAGAAG TGAAGAAAAA GAAAGTAAAG ATCAAGAAAC AAATGATGAC 
CTAAAAACTG AAAGTGGACA GGAGAAAAAG AGAAGAAAGA AAAAGAAGAA GCTTCGTTCA 
AAGAAGACAA GAGAGCATGA GCTGAGATTT TTGAAGAATA GCGGAAATCA AGCATCTCCC 
CTTCTTATGG ATTGCTCTAC ACAATATGAG ATATTTACAG TGGAAAGTGG GTCGCAATAC 
GATGTGAAAG GAAGAAGCCG AAAATGTCAG ACAAAACTTT CCAGTATAAA TCATGAGCAG 
TTACAAGGGA TTCGAGAAAC AGTTCAAAAG TTGAGAAGAA GAGAAAGTGA AATGAGTAAG 
GTGTCGAATG TTTCTGAAAG TGACTGGGAT AGTGAAACTG GAAAAGAACC GGAAGTCGCT 
GAAATCAGTT TAGATCTTTC TGTAGTTGCT CTCGGAGTTG ATGAATCCGG GAAAGTTGTT 
GATATTCCTA AGGAAGTACC TAAAGCTGTT CAACGACATG AATCATTTTC ACCAACATCG 
GAGCTCACCA CTGCGTCCGA ATCACTTTCC GCTGAGAAAG TACATCTGGA TTCAGAAGAT 
GAGGAGTTAG AAAGTCTTGA AGAACTCGAA CTGTATACCA CAGACGCCGG AATTCAGACT 
GAAACAAAGG TCATGCTGGA CAAGCAAGAT AATGCAACTC CAAAGTTGCT GGAAATTATT 
AAAAACTGCA GCACTCAGAC AACATTCAGC ACATTCCACA TTCCGCAGAA GGTATATGAG 
TCGGCGAGCA CCTCAAGTGA CGAGGAAGAT CATAAAAACG ATGGTGAAGA TGAAAAACAG 
AAGAAATCTA AACAGGAAGT GTTCTTTATG ACCAACACTG GAGTACAGAC GATGCATACA 
TCGTTCCATA TTGATGAAAA ACCGAAATTT GTTGAAGAAA ATCTTCAAAC TCAAAATACC 
CACGACGGCA TAAAAGAACA AAGTGCCGTT GATGAAGAAA TGCCTCTGTT CTTAAAAAAT 
TCGGATTGTC AAACCGTTGA AGTAATATCA TTCGAATGTG CCACATCAAC AGAGAATGTT 
AGTTTTGTTG GAGACGTTGA AACTTCTGAA GGATCCAGTC AGACAGCCAT GCAAGTTTCT 
GAAACTGAAG TACAAACAAT GCAACAAGAA TTTGTGGTAG CAACAATGCA AACCGACGAG 
CCACTGTTGG TATCTGATGA GTCACAGACT GAGCAAACAA AATCTTCGGA AATTGATTCT 
CAAACTGATC CACCATCCAC AAAATCATTT ATATGTCAGA CAGAATTGAT AACAAGCTCG 
GAAGTTGAAG TTCAAGCAGT TCGAGCTGTA GAGTTTTCAC TTGTCGAATG CCAAACAGAT 
GCGCTAAATC ATGAAGAAGT AGAAGTTCAA ACAGAACTGG ATATTTCGGT TTCGCAGTCC 
CAAACTGATA ATGTTGATGT AGGCCACATT GAAATGCAAA CTGATATGAC AATAACAAAT 
GCATCGGAAT CTCAAACCGA TGTGCTCAGT GTAGGGGACA CGGAGATGCA AACAGACGAG 
AAAGAAATAT TCACAATAGA ATCGCAAACC GAACAACCAT CCACAGAACT GCAAGAGACT 
CAAACTGACG ATGTTTCTTT TGCTGGCGAT GTTCCAACAA CCGACCAAAT CACACAATCG 
GAATCTGCTC AAGTTTTCGA TTCTGAAACT CAATATGCTT TATCCATAGA AGAAAAGGAC 
GTCCAGGCAA CGCCAATCTC ATTTGAAACT CAGACACAAC ATTCCATTGA ACTTGAAGTG 
AAATCTTGCC AAACCGAAGG AGTTCGAATG AAAAACAAAA AGAGTCAGGT GTATGTTCAA 
GAACTCGATG AAGAACGTCG AATGCGTATG TCAGACATTG GCATACAAAC CGGTGTTCTG 
GCACGTGTTC AACACATTTA TACCCGTCCA ATATCTGATG ATGTGGGACC ATCTTCATCT 
GCTCCAATCA CTCTTCGAAA GTCTTCCGAC TCCTCGGAAC ACATATCAAC GAAAACTGCG 
AAAAGCAGCG AAGAGTCATT GGCGCCATCC GCTTCCGGGA CAGCGCTCTC TGCAGCTGAA 
TCAATGGATG ATCATTTATG GCCAGAGAGA GCAAGTCCAT TTGCATCGAG GCATGGCTCA 
TTGAAGAGAA GAACGAGGGA ACTTGATCCC GAGAAAAAGG GGGACAAAAC AAATATTGTT 
CAAGATCTAC GTGCAAGATT TGAAATGAAA TCTCCACCAA AATCACCACC GAAAGATGAT 
GAAGAAGGAA CATCTACGGA TCCCAAACAT TTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twk-11 ( NM_001356689.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001343632.1 Caenorhabditis elegans TWiK family of potassium channels (twk-11), mRNA.

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CloneID	OCa529871
Clone ID Related Accession (Same CDS sequence)	NM_001356689.1 , NM_001356689.2
Accession Version	NM_001356689.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	TWiK family of potassium channels
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001356689.1.

ATGTCCAATG ATAACTTGGC GATTGAAATC GACCCTCCGG ACGATGTGGA GTATGATGTG 
GAAGCAGGCT TCAACTCTTC CACATGCCAT TTAATGCACA ATTTCGAAGA GGAACGTCCA 
ACCTCTCTCG TAGCCTCCAT AAAGGAAAGT GCCATTAGCT CAAAGCGAAA ACTAAAATAC 
TACTGCTCGT TCATATGCAC CCCAATCAAG AAGTTGTTAT CCACTTTCAA ACTACTCATC 
ATAATCGGTC TTTACTCATT TATTGGCGCT CATATATTCA TGTACTTGGA AGTTCCAACT 
GATTTAGAAG CAAGAGAAGA TGGATTTCAT CAAAGAAAAA TAGCGAGGGA AGTGATGGTG 
CTGAATCTCC GTGCAATTTT CTACGACAAC CGCGAAGATC GCGAGGAACG ATGGAAGCAT 
GCCATTTTGA AGTTCGAAGA GGACATTGGC TTAGAAGAAC CTGTCATTGA AACTGTATGG 
ACTTTTTGGA TGTCTTTCCT CTATGCAGGA ACTATTTTCA CAACAATAGG ATATGGAAAC 
ATTGCATGTA AGACGCGTGC TGGCCAGATA GCTACAATGG TATATGCTTT TGTCGGAATT 
CCGATCATGC TGGTGATGCT CACCTCGCTC AACAATTTTT TGTTAAAATG GATAAAACTT 
ATTACGAATG GAGTTAGTGA TATGACGCTT TATATTGGTG TTCGCTTGGG AATAACAGTT 
ATTCGGCAGG ACGAAGTCCA GAAGCGCCTT CGCTACACAA AACTTGCCAA GACAATGAAA 
AGGTGGAAAC TTTCAAAACA CGGAGCTCCA AGTAGTATAG CGATTAGCAA TTCGGAAGAA 
AACCGGTTAA ATTCAACACC TGAAGACGAC GAAGAAGAGG AAGAAATTCA TCAGGATCCT 
CCAGTTCTTT CGACTCTAAT TGCAACTGTT GCCTGGATTA TTCTTTCGGC TGCTGTGTTT 
TGTCTTTTTG AGGACTGGAC ATTTTTCACC AGTTTTTATT TTTGTTTCAT CTCTCTGACA 
ACAATCGGTC TTGGAGATGT GACACCTGCT AATCCAGAAT ATATGATTGC AACATTTGGA 
GTTGTGATTG TCGGTTTATC AATGCTCACT GTCTGTATCG ACGTCTTACA AGAAAAACTT 
GCTCAAATGT ATATGGCTCT ACTTCAGAAG TTATTGACAG AGTACATGGA AGCTGTGAAA 
AGCGGAGACC CAAATGCTGC ATCAGCTATG ATGGCTGGTT TTCAAGGAAG GGCAAAGTTT 
TTGATGCCGT TGATTAGTAA AAACGAAGGA GCAAAAGTTA TGGATAAGTT CAAACAAGAT 
TGTTCAAAGA AGGGAATCGA TCCACCACCG GTTTTAACAA ATATCAACCC GGAGACCGGA 
ATGCCAGCTT TTGCAAATGC TCCGAAAGAA GATTTTAGAG ATTATATTGA AGTTGCAGAT 
GAAAAATTTG CTGATGAACA ACAGAGGCTC TCTCAAACCC CGTTATCAAA ATCCACCGAC 
TTTTTACAAT CACCCACACC ACGTCCAACT GTCACTGTAG CTGTTACTCA AACAGCTAGA 
CCACAGGCAT GCGTATCACT AGAAGTACAG GCTGGAACCA CAATGGGAAA GTTCCATGAT 
TATGGATCAC AGACACAGAC GGAAAGTTCT GACGAAGGAA TTCAGACGGA TTATAGCATT 
GATTTTGATG ATGAAAAAAA CAAGGAGCCA GAGAAAGTTA TGGCGGACAT TGGCACTCAA 
TATGAAATCG TTCTATTAAC AAGTATTGGA ATTCAACCAG AAGTCTCATC AATTTATATT 
GGAGCCGGCT TATCTGATCA TTCGGACGAA GAAAGTGGAT GTGAATCAGA AGACGATTCG 
GATGACTCGG ATGTATCATC CGATGAGGAA AGTGAACTTA AATCTTCTGC GAAATACAAA 
GAAACACCTC TTCCATTTGA AGTCGAAGAT CCAGGAATGA TGTCGCTCGG ATTACAGTCT 
CCACCAATTG AAATAACTCC CTTCACGGAT GATGAATCAG CTGCAAGCGA CGCAAGAAGT 
TCTCCTGTCC CACCAACATC TTCCAAGCGA TCCAAGTTGA CACCAAAGAC TATAAAAGTT 
GGCAAATCGT TATCAATGGA CTCGACTGAT ACAGTTATTC ATGTGAAACC AGTTGGCGTT 
AATGATGATG AGAAAAGAAG TGAAGAAAAA GAAAGTAAAG ATCAAGAAAC AAATGATGAC 
CTAAAAACTG AAAGTGGACA GGAGAAAAAG AGAAGAAAGA AAAAGAAGAA GCTTCGTTCA 
AAGAAGACAA GAGAGCATGA GCTGAGATTT TTGAAGAATA GCGGAAATCA AGCATCTCCC 
CTTCTTATGG ATTGCTCTAC ACAATATGAG ATATTTACAG TGGAAAGTGG GTCGCAATAC 
GATGTGAAAG GAAGAAGCCG AAAATGTCAG ACAAAACTTT CCAGTATAAA TCATGAGCAG 
TTACAAGGGA TTCGAGAAAC AGTTCAAAAG TTGAGAAGAA GAGAAAGTGA AATGAGTAAG 
GTGTCGAATG TTTCTGAAAG TGACTGGGAT AGTGAAACTG GAAAAGAACC GGAAGTCGCT 
GAAATCAGTT TAGATCTTTC TGTAGTTGCT CTCGGAGTTG ATGAATCCGG GAAAGTTGTT 
GATATTCCTA AGGAAGTACC TAAAGCTGTT CAACGACATG AATCATTTTC ACCAACATCG 
GAGCTCACCA CTGCGTCCGA ATCACTTTCC GCTGAGAAAG TACATCTGGA TTCAGAAGAT 
GAGGAGTTAG AAAGTCTTGA AGAACTCGAA CTGTATACCA CAGACGCCGG AATTCAGACT 
GAAACAAAGG TCATGCTGGA CAAGCAAGAT AATGCAACTC CAAAGTTGCT GGAAATTATT 
AAAAACTGCA GCACTCAGAC AACATTCAGC ACATTCCACA TTCCGCAGAA GGTATATGAG 
TCGGCGAGCA CCTCAAGTGA CGAGGAAGAT CATAAAAACG ATGGTGAAGA TGAAAAACAG 
AAGAAATCTA AACAGGAAGT GTTCTTTATG ACCAACACTG GAGTACAGAC GATGCATACA 
TCGTTCCATA TTGATGAAAA ACCGAAATTT GTTGAAGAAA ATCTTCAAAC TCAAAATACC 
CACGACGGCA TAAAAGAACA AAGTGCCGTT GATGAAGAAA TGCCTCTGTT CTTAAAAAAT 
TCGGATTGTC AAACCGTTGA AGTAATATCA TTCGAATGTG CCACATCAAC AGAGAATGTT 
AGTTTTGTTG GAGACGTTGA AACTTCTGAA GGATCCAGTC AGACAGCCAT GCAAGTTTCT 
GAAACTGAAG TACAAACAAT GCAACAAGAA TTTGTGGTAG CAACAATGCA AACCGACGAG 
CCACTGTTGG TATCTGATGA GTCACAGACT GAGCAAACAA AATCTTCGGA AATTGATTCT 
CAAACTGATC CACCATCCAC AAAATCATTT ATATGTCAGA CAGAATTGAT AACAAGCTCG 
GAAGTTGAAG TTCAAGCAGT TCGAGCTGTA GAGTTTTCAC TTGTCGAATG CCAAACAGAT 
GCGCTAAATC ATGAAGAAGT AGAAGTTCAA ACAGAACTGG ATATTTCGGT TTCGCAGTCC 
CAAACTGATA ATGTTGATGT AGGCCACATT GAAATGCAAA CTGATATGAC AATAACAAAT 
GCATCGGAAT CTCAAACCGA TGTGCTCAGT GTAGGGGACA CGGAGATGCA AACAGACGAG 
AAAGAAATAT TCACAATAGA ATCGCAAACC GAACAACCAT CCACAGAACT GCAAGAGACT 
CAAACTGACG ATGTTTCTTT TGCTGGCGAT GTTCCAACAA CCGACCAAAT CACACAATCG 
GAATCTGCTC AAGTTTTCGA TTCTGAAACT CAATATGCTT TATCCATAGA AGAAAAGGAC 
GTCCAGGCAA CGCCAATCTC ATTTGAAACT CAGACACAAC ATTCCATTGA ACTTGAAGTG 
AAATCTTGCC AAACCGAAGG AGTTCGAATG AAAAACAAAA AGAGTCAGGT GTATGTTCAA 
GAACTCGATG AAGAACGTCG AATGCGTATG TCAGACATTG GCATACAAAC CGGTGTTCTG 
GCACGTGTTC AACACATTTA TACCCGTCCA ATATCTGATG ATGTGGGACC ATCTTCATCT 
GCTCCAATCA CTCTTCGAAA GTCTTCCGAC TCCTCGGAAC ACATATCAAC GAAAACTGCG 
AAAAGCAGCG AAGAGTCATT GGCGCCATCC GCTTCCGGGA CAGCGCTCTC TGCAGCTGAA 
TCAATGGATG ATCATTTATG GCCAGAGAGA GCAAGTCCAT TTGCATCGAG GCATGGCTCA 
TTGAAGAGAA GAACGAGGGA ACTTGATCCC GAGAAAAAGG GGGACAAAAC AAATATTGTT 
CAAGATCTAC GTGCAAGATT TGAAATGAAA TCTCCACCAA AATCACCACC GAAAGATGAT 
GAAGAAGGAA CATCTACGGA TCCCAAACAT TTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343632.1
CDS	129..4607
Translation	MSNDNLAIEIDPPDDVEYDVEAGFNSSTCHLMHNFEEERPTSLVASIKESAISSKRKLKYYCSFICTPIKKLLSTFKLLIIIGLYSFIGAHIFMYLEVPTDLEAREDGFHQRKIAREVMVLNLRAIFYDNREDREERWKHAILKFEEDIGLEEPVIETVWTFWMSFLYAGTIFTTIGYGNIACKTRAGQIATMVYAFVGIPIMLVMLTSLNNFLLKWIKLITNGVSDMTLYIGVRLGITVIRQDEVQKRLRYTKLAKTMKRWKLSKHGAPSSIAISNSEENRLNSTPEDDEEEEEIHQDPPVLSTLIATVAWIILSAAVFCLFEDWTFFTSFYFCFISLTTIGLGDVTPANPEYMIATFGVVIVGLSMLTVCIDVLQEKLAQMYMALLQKLLTEYMEAVKSGDPNAASAMMAGFQGRAKFLMPLISKNEGAKVMDKFKQDCSKKGIDPPPVLTNINPETGMPAFANAPKEDFRDYIEVADEKFADEQQRLSQTPLSKSTDFLQSPTPRPTVTVAVTQTARPQACVSLEVQAGTTMGKFHDYGSQTQTESSDEGIQTDYSIDFDDEKNKEPEKVMADIGTQYEIVLLTSIGIQPEVSSIYIGAGLSDHSDEESGCESEDDSDDSDVSSDEESELKSSAKYKETPLPFEVEDPGMMSLGLQSPPIEITPFTDDESAASDARSSPVPPTSSKRSKLTPKTIKVGKSLSMDSTDTVIHVKPVGVNDDEKRSEEKESKDQETNDDLKTESGQEKKRRKKKKKLRSKKTREHELRFLKNSGNQASPLLMDCSTQYEIFTVESGSQYDVKGRSRKCQTKLSSINHEQLQGIRETVQKLRRRESEMSKVSNVSESDWDSETGKEPEVAEISLDLSVVALGVDESGKVVDIPKEVPKAVQRHESFSPTSELTTASESLSAEKVHLDSEDEELESLEELELYTTDAGIQTETKVMLDKQDNATPKLLEIIKNCSTQTTFSTFHIPQKVYESASTSSDEEDHKNDGEDEKQKKSKQEVFFMTNTGVQTMHTSFHIDEKPKFVEENLQTQNTHDGIKEQSAVDEEMPLFLKNSDCQTVEVISFECATSTENVSFVGDVETSEGSSQTAMQVSETEVQTMQQEFVVATMQTDEPLLVSDESQTEQTKSSEIDSQTDPPSTKSFICQTELITSSEVEVQAVRAVEFSLVECQTDALNHEEVEVQTELDISVSQSQTDNVDVGHIEMQTDMTITNASESQTDVLSVGDTEMQTDEKEIFTIESQTEQPSTELQETQTDDVSFAGDVPTTDQITQSESAQVFDSETQYALSIEEKDVQATPISFETQTQHSIELEVKSCQTEGVRMKNKKSQVYVQELDEERRMRMSDIGIQTGVLARVQHIYTRPISDDVGPSSSAPITLRKSSDSSEHISTKTAKSSEESLAPSASGTALSAAESMDDHLWPERASPFASRHGSLKRRTRELDPEKKGDKTNIVQDLRARFEMKSPPKSPPKDDEEGTSTDPKHFF

Target ORF information:

RefSeq Version	NM_001356689.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TWiK family of potassium channels (twk-11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356689.2

ORF Insert Sequence:

ATGTCCAATG ATAACTTGGC GATTGAAATC GACCCTCCGG ACGATGTGGA GTATGATGTG 
GAAGCAGGCT TCAACTCTTC CACATGCCAT TTAATGCACA ATTTCGAAGA GGAACGTCCA 
ACCTCTCTCG TAGCCTCCAT AAAGGAAAGT GCCATTAGCT CAAAGCGAAA ACTAAAATAC 
TACTGCTCGT TCATATGCAC CCCAATCAAG AAGTTGTTAT CCACTTTCAA ACTACTCATC 
ATAATCGGTC TTTACTCATT TATTGGCGCT CATATATTCA TGTACTTGGA AGTTCCAACT 
GATTTAGAAG CAAGAGAAGA TGGATTTCAT CAAAGAAAAA TAGCGAGGGA AGTGATGGTG 
CTGAATCTCC GTGCAATTTT CTACGACAAC CGCGAAGATC GCGAGGAACG ATGGAAGCAT 
GCCATTTTGA AGTTCGAAGA GGACATTGGC TTAGAAGAAC CTGTCATTGA AACTGTATGG 
ACTTTTTGGA TGTCTTTCCT CTATGCAGGA ACTATTTTCA CAACAATAGG ATATGGAAAC 
ATTGCATGTA AGACGCGTGC TGGCCAGATA GCTACAATGG TATATGCTTT TGTCGGAATT 
CCGATCATGC TGGTGATGCT CACCTCGCTC AACAATTTTT TGTTAAAATG GATAAAACTT 
ATTACGAATG GAGTTAGTGA TATGACGCTT TATATTGGTG TTCGCTTGGG AATAACAGTT 
ATTCGGCAGG ACGAAGTCCA GAAGCGCCTT CGCTACACAA AACTTGCCAA GACAATGAAA 
AGGTGGAAAC TTTCAAAACA CGGAGCTCCA AGTAGTATAG CGATTAGCAA TTCGGAAGAA 
AACCGGTTAA ATTCAACACC TGAAGACGAC GAAGAAGAGG AAGAAATTCA TCAGGATCCT 
CCAGTTCTTT CGACTCTAAT TGCAACTGTT GCCTGGATTA TTCTTTCGGC TGCTGTGTTT 
TGTCTTTTTG AGGACTGGAC ATTTTTCACC AGTTTTTATT TTTGTTTCAT CTCTCTGACA 
ACAATCGGTC TTGGAGATGT GACACCTGCT AATCCAGAAT ATATGATTGC AACATTTGGA 
GTTGTGATTG TCGGTTTATC AATGCTCACT GTCTGTATCG ACGTCTTACA AGAAAAACTT 
GCTCAAATGT ATATGGCTCT ACTTCAGAAG TTATTGACAG AGTACATGGA AGCTGTGAAA 
AGCGGAGACC CAAATGCTGC ATCAGCTATG ATGGCTGGTT TTCAAGGAAG GGCAAAGTTT 
TTGATGCCGT TGATTAGTAA AAACGAAGGA GCAAAAGTTA TGGATAAGTT CAAACAAGAT 
TGTTCAAAGA AGGGAATCGA TCCACCACCG GTTTTAACAA ATATCAACCC GGAGACCGGA 
ATGCCAGCTT TTGCAAATGC TCCGAAAGAA GATTTTAGAG ATTATATTGA AGTTGCAGAT 
GAAAAATTTG CTGATGAACA ACAGAGGCTC TCTCAAACCC CGTTATCAAA ATCCACCGAC 
TTTTTACAAT CACCCACACC ACGTCCAACT GTCACTGTAG CTGTTACTCA AACAGCTAGA 
CCACAGGCAT GCGTATCACT AGAAGTACAG GCTGGAACCA CAATGGGAAA GTTCCATGAT 
TATGGATCAC AGACACAGAC GGAAAGTTCT GACGAAGGAA TTCAGACGGA TTATAGCATT 
GATTTTGATG ATGAAAAAAA CAAGGAGCCA GAGAAAGTTA TGGCGGACAT TGGCACTCAA 
TATGAAATCG TTCTATTAAC AAGTATTGGA ATTCAACCAG AAGTCTCATC AATTTATATT 
GGAGCCGGCT TATCTGATCA TTCGGACGAA GAAAGTGGAT GTGAATCAGA AGACGATTCG 
GATGACTCGG ATGTATCATC CGATGAGGAA AGTGAACTTA AATCTTCTGC GAAATACAAA 
GAAACACCTC TTCCATTTGA AGTCGAAGAT CCAGGAATGA TGTCGCTCGG ATTACAGTCT 
CCACCAATTG AAATAACTCC CTTCACGGAT GATGAATCAG CTGCAAGCGA CGCAAGAAGT 
TCTCCTGTCC CACCAACATC TTCCAAGCGA TCCAAGTTGA CACCAAAGAC TATAAAAGTT 
GGCAAATCGT TATCAATGGA CTCGACTGAT ACAGTTATTC ATGTGAAACC AGTTGGCGTT 
AATGATGATG AGAAAAGAAG TGAAGAAAAA GAAAGTAAAG ATCAAGAAAC AAATGATGAC 
CTAAAAACTG AAAGTGGACA GGAGAAAAAG AGAAGAAAGA AAAAGAAGAA GCTTCGTTCA 
AAGAAGACAA GAGAGCATGA GCTGAGATTT TTGAAGAATA GCGGAAATCA AGCATCTCCC 
CTTCTTATGG ATTGCTCTAC ACAATATGAG ATATTTACAG TGGAAAGTGG GTCGCAATAC 
GATGTGAAAG GAAGAAGCCG AAAATGTCAG ACAAAACTTT CCAGTATAAA TCATGAGCAG 
TTACAAGGGA TTCGAGAAAC AGTTCAAAAG TTGAGAAGAA GAGAAAGTGA AATGAGTAAG 
GTGTCGAATG TTTCTGAAAG TGACTGGGAT AGTGAAACTG GAAAAGAACC GGAAGTCGCT 
GAAATCAGTT TAGATCTTTC TGTAGTTGCT CTCGGAGTTG ATGAATCCGG GAAAGTTGTT 
GATATTCCTA AGGAAGTACC TAAAGCTGTT CAACGACATG AATCATTTTC ACCAACATCG 
GAGCTCACCA CTGCGTCCGA ATCACTTTCC GCTGAGAAAG TACATCTGGA TTCAGAAGAT 
GAGGAGTTAG AAAGTCTTGA AGAACTCGAA CTGTATACCA CAGACGCCGG AATTCAGACT 
GAAACAAAGG TCATGCTGGA CAAGCAAGAT AATGCAACTC CAAAGTTGCT GGAAATTATT 
AAAAACTGCA GCACTCAGAC AACATTCAGC ACATTCCACA TTCCGCAGAA GGTATATGAG 
TCGGCGAGCA CCTCAAGTGA CGAGGAAGAT CATAAAAACG ATGGTGAAGA TGAAAAACAG 
AAGAAATCTA AACAGGAAGT GTTCTTTATG ACCAACACTG GAGTACAGAC GATGCATACA 
TCGTTCCATA TTGATGAAAA ACCGAAATTT GTTGAAGAAA ATCTTCAAAC TCAAAATACC 
CACGACGGCA TAAAAGAACA AAGTGCCGTT GATGAAGAAA TGCCTCTGTT CTTAAAAAAT 
TCGGATTGTC AAACCGTTGA AGTAATATCA TTCGAATGTG CCACATCAAC AGAGAATGTT 
AGTTTTGTTG GAGACGTTGA AACTTCTGAA GGATCCAGTC AGACAGCCAT GCAAGTTTCT 
GAAACTGAAG TACAAACAAT GCAACAAGAA TTTGTGGTAG CAACAATGCA AACCGACGAG 
CCACTGTTGG TATCTGATGA GTCACAGACT GAGCAAACAA AATCTTCGGA AATTGATTCT 
CAAACTGATC CACCATCCAC AAAATCATTT ATATGTCAGA CAGAATTGAT AACAAGCTCG 
GAAGTTGAAG TTCAAGCAGT TCGAGCTGTA GAGTTTTCAC TTGTCGAATG CCAAACAGAT 
GCGCTAAATC ATGAAGAAGT AGAAGTTCAA ACAGAACTGG ATATTTCGGT TTCGCAGTCC 
CAAACTGATA ATGTTGATGT AGGCCACATT GAAATGCAAA CTGATATGAC AATAACAAAT 
GCATCGGAAT CTCAAACCGA TGTGCTCAGT GTAGGGGACA CGGAGATGCA AACAGACGAG 
AAAGAAATAT TCACAATAGA ATCGCAAACC GAACAACCAT CCACAGAACT GCAAGAGACT 
CAAACTGACG ATGTTTCTTT TGCTGGCGAT GTTCCAACAA CCGACCAAAT CACACAATCG 
GAATCTGCTC AAGTTTTCGA TTCTGAAACT CAATATGCTT TATCCATAGA AGAAAAGGAC 
GTCCAGGCAA CGCCAATCTC ATTTGAAACT CAGACACAAC ATTCCATTGA ACTTGAAGTG 
AAATCTTGCC AAACCGAAGG AGTTCGAATG AAAAACAAAA AGAGTCAGGT GTATGTTCAA 
GAACTCGATG AAGAACGTCG AATGCGTATG TCAGACATTG GCATACAAAC CGGTGTTCTG 
GCACGTGTTC AACACATTTA TACCCGTCCA ATATCTGATG ATGTGGGACC ATCTTCATCT 
GCTCCAATCA CTCTTCGAAA GTCTTCCGAC TCCTCGGAAC ACATATCAAC GAAAACTGCG 
AAAAGCAGCG AAGAGTCATT GGCGCCATCC GCTTCCGGGA CAGCGCTCTC TGCAGCTGAA 
TCAATGGATG ATCATTTATG GCCAGAGAGA GCAAGTCCAT TTGCATCGAG GCATGGCTCA 
TTGAAGAGAA GAACGAGGGA ACTTGATCCC GAGAAAAAGG GGGACAAAAC AAATATTGTT 
CAAGATCTAC GTGCAAGATT TGAAATGAAA TCTCCACCAA AATCACCACC GAAAGATGAT 
GAAGAAGGAA CATCTACGGA TCCCAAACAT TTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twk-11 ( NM_072381.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_504782.2 Caenorhabditis elegans TWiK family of potassium channels (twk-11), partial mRNA.

Price & Availability↑

CloneID	OCa347226
Clone ID Related Accession (Same CDS sequence)	NM_072381.5
Accession Version	NM_072381.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-12
Organism	Caenorhabditis elegans
Product	TWiK family of potassium channels
Comment	REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_072381.4. COMPLETENESS: incomplete on both ends.

ATGTCCAATG ATAACTTGGC GATTGAAATC GACCCTCCGG ACGATGTGGA GTATGATGTG 
GAAGCAGGCT TCAACTCTTC CACATGCCAT TTAATGCACA ATTTCGAAGA GGAACGTCCA 
ACCTCTCTCG TAGCCTCCAT AAAGGAAAGT GCCATTAGCT CAAAGCGAAA ACTAAAATAC 
TACTGCTCGT TCATATGCAC CCCAATCAAG AAGTTGTTAT CCACTTTCAA ACTACTCATC 
ATAATCGGTC TTTACTCATT TATTGGCGCT CATATATTCA TGTACTTGGA AGTTCCAACT 
GATTTAGAAG CAAGAGAAGA TGGATTTCAT CAAAGAAAAA TAGCGAGGGA AGTGATGGTG 
CTGAATCTCC GTGCAATTTT CTACGACAAC CGCGAAGATC GCGAGGAACG ATGGAAGCAT 
GCCATTTTGA AGTTCGAAGA GGACATTGGC TTAGAAGAAC CTGTCATTGA AACTGTATGG 
ACTTTTTGGA TGTCTTTCCT CTATGCAGGA ACTATTTTCA CAACAATAGG ATATGGAAAC 
ATTGCATGTA AGACGCGTGC TGGCCAGATA GCTACAATGG TATATGCTTT TGTCGGAATT 
CCGATCATGC TGGTGATGCT CACCTCGCTC AACAATTTTT TGTTAAAATG GATAAAACTT 
ATTACGAATG GAGTTAGTGA TATGACGCTT TATATTGGTG TTCGCTTGGG AATAACAGTT 
ATTCGGCAGG ACGAAGTCCA GAAGCGCCTT CGCTACACAA AACTTGCCAA GACAATGAAA 
AGGTGGAAAC TTTCAAAACA CGGAGCTCCA AGTAGTATAG CGATTAGCAA TTCGGAAGAA 
AACCGGTTAA ATTCAACACC TGAAGACGAC GAAGAAGAGG AAGAAATTCA TCAGGATCCT 
CCAGTTCTTT CGACTCTAAT TGCAACTGTT GCCTGGATTA TTCTTTCGGC TGCTGTGTTT 
TGTCTTTTTG AGGACTGGAC ATTTTTCACC AGTTTTTATT TTTGTTTCAT CTCTCTGACA 
ACAATCGGTC TTGGAGATGT GACACCTGCT AATCCAGAAT ATATGATTGC AACATTTGGA 
GTTGTGATTG TCGGTTTATC AATGCTCACT GTCTGTATCG ACGTCTTACA AGAAAAACTT 
GCTCAAATGT ATATGGCTCT ACTTCAGAAG TTATTGACAG AGTACATGGA AGCTGTGAAA 
AGCGGAGACC CAAATGCTGC ATCAGCTATG ATGGCTGGTT TTCAAGGAAG GGCAAAGTTT 
TTGATGCCGT TGATTAGTAA AAACGAAGGA GCAAAAGTTA TGGATAAGTT CAAACAAGAT 
TGTTCAAAGA AGGGAATCGA TCCACCACCG GTTTTAACAA ATATCAACCC GGAGACCGGA 
ATGCCAGCTT TTGCAAATGC TCCGAAAGAA GATTTTAGAG ATTATATTGA AGTTGCAGAT 
GAAAAATTTG CTGATGAACA ACAGAGGCTC TCTCAAACCC CGTTATCAAA ATCCACCGAC 
TTTTTACAAT CACCCACACC ACGTCCAACT GTCACTGTAG CTGTTACTCA AACAGCTAGA 
CCACAGGCAT GCGTATCACT AGAAGTACAG GCTGGAACCA CAATGGGAAA GTTCCATGAT 
TATGGATCAC AGACACAGAC GGAAAGTTCT GACGAAGGAA TTCAGACGGA TTATAGCATT 
GATTTTGATG ATGAAAAAAA CAAGGAGCCA GAGAAAGTTA TGGCGGACAT TGGCACTCAA 
TATGAAATCG TTCTATTAAC AAGTATTGGA ATTCAACCAG AAGTCTCATC AATTTATATT 
GGAGCCGGCT TATCTGATCA TTCGGACGAA GAAAGTGGAT GTGAATCAGA AGACGATTCG 
GATGACTCGG ATGTATCATC CGATGAGGAA AGTGAACTTA AATCTTCTGC GAAATACAAA 
GAAACACCTC TTCCATTTGA AGTCGAAGAT CCAGGAATGA TGTCGCTCGG ATTACAGTCT 
CCACCAATTG AAATAACTCC CTTCACGGAT GATGAATCAG CTGCAAGCGA CGCAAGAAGT 
TCTCCTGTCC CACCAACATC TTCCAAGCGA TCCAAGTTGA CACCAAAGAC TATAAAAGTT 
GGCAAATCGT TATCAATGGA CTCGACTGAT ACAGTTATTC ATGTGAAACC AGTTGGCGTT 
AATGATGATG AGAAAAGAAG TGAAGAAAAA GAAAGTAAAG ATCAAGAAAC AAATGATGAC 
CTAAAAACTG AAAGTGGACA GGAGAAAAAG AGAAGAAAGA AAAAGAAGAA GCTTCGTTCA 
AAGAAGACAA GAGAGCATGA GCTGAGATTT TTGAAGAATA GCGGAAATCA AGCATCTCCC 
CTTCTTATGG ATTGCTCTAC ACAATATGAG ATATTTACAG TGGAAAGTGG GTCGCAATAC 
GATGTGAAAG GAAGAAGCCG AAAATGTCAG ACAAAACTTT CCAGTATAAA TCATGAGCAG 
TTACAAGGGA TTCGAGAAAC AGTTCAAAAG TTGAGAAGAA GAGAAAGTGA AATGAGTAAG 
GTGTCGAATG TTTCTGAAAG TGACTGGGAT AGTGAAACTG GAAAAGAACC GGAAGTCGCT 
GAAATCAGTT TAGATCTTTC TGTAGTTGCT CTCGGAGTTG ATGAATCCGG GAAAGTTGTT 
GATATTCCTA AGGAAGTACC TAAAGCTGTT CAACGACATG AATCATTTTC ACCAACATCG 
GAGCTCACCA CTGCGTCCGA ATCACTTTCC GCTGAGAAAG TACATCTGGA TTCAGAAGAT 
GAGGAGTTAG AAAGTCTTGA AGAACTCGAA CTGTATACCA CAGACGCCGG AATTCAGACT 
GAAACAAAGG TCATGCTGGA CAAGCAAGAT AATGCAACTC CAAAGTTGCT GGAAATTATT 
AAAAACTGCA GCACTCAGAC AACATTCAGC ACATTCCACA TTCCGCAGAA GGTATATGAG 
TCGGCGAGCA CCTCAAGTGA CGAGGAAGAT CATAAAAACG ATGGTGAAGA TGAAAAACAG 
AAGAAATCTA AACAGGAAGT GTTCTTTATG ACCAACACTG GAGTACAGAC GATGCATACA 
TCGTTCCATA TTGATGAAAA ACCGAAATTT GTTGAAGAAA ATCTTCAAAC TCAAAATACC 
CACGACGGCA TAAAAGAACA AAGTGCCGTT GATGAAGAAA TGCCTCTGTT CTTAAAAAAT 
TCGGATTGTC AAACCGTTGA AGTAATATCA TTCGAATGTG CCACATCAAC AGAGAATGTT 
AGTTTTGTTG GAGACGTTGA AACTTCTGAA GGATCCAGTC AGACAGCCAT GCAAGTTTCT 
GAAACTGAAG TACAAACAAT GCAACAAGAA TTTGTGGTAG CAACAATGCA AACCGACGAG 
CCACTGTTGG TATCTGATGA GTCACAGACT GAGCAAACAA AATCTTCGGA AATTGATTCT 
CAAACTGATC CACCATCCAC AAAATCATTT ATATGTCAGA CAGAATTGAT AACAAGCTCG 
GAAGTTGAAG TTCAAGCAGT TCGAGCTGTA GAGTTTTCAC TTGTCGAATG CCAAACAGAT 
GCGCTAAATC ATGAAGAAGT AGAAGTTCAA ACAGAACTGG ATATTTCGGT TTCGCAGTCC 
CAAACTGATA ATGTTGATGT AGGCCACATT GAAATGCAAA CTGATATGAC AATAACAAAT 
GCATCGGAAT CTCAAACCGA TGTGCTCAGT GTAGGGGACA CGGAGATGCA AACAGACGAG 
GTGGATTCTA TTATTTTCAA ACTGATTTTC GTGGATGTGC ACGAAATTAC ATTTTTTCGA 
AAATTGATAA GACATTCACA TACAAAATTT GGTCCGGTAG TTGTTCTTAT GTTAGTTGAG 
ATATTTCAAT CTGAGAAATC AACCAATTTT TGGAAAAAAG AAATATTCAC AATAGAATCG 
CAAACCGAAC AACCATCCAC AGAACTGCAA GAGACTCAAA CTGACGATGT TTCTTTTGCT 
GGCGATGTTC CAACAACCGA CCAAATCACA CAATCGGAAT CTGCTCAAGT TTTCGATTCT 
GAAACTCAAT ATGCTTTATC CATAGAAGAA AAGGACGTCC AGGCAACGCC AATCTCATTT 
GAAACTCAGA CACAACATTC CATTGAACTT GAAGTGAAAT CTTGCCAAAC CGAAGGAGTT 
CGAATGAAAA ACAAAAAGAG TCAGGTGTAT GTTCAAGAAC TCGATGAAGA ACGTCGAATG 
CGTATGTCAG ACATTGGCAT ACAAACCGGT GTTCTGGCAC GTGTTCAACA CATTTATACC 
CGTCCAATAT CTGATGATGT GGGACCATCT TCATCTGCTC CAATCACTCT TCGAAAGTCT 
TCCGACTCCT CGGAACACAT ATCAACGAAA ACTGCGAAAA GCAGCGAAGA GTCATTGGCG 
CCATCCGCTT CCGGGACAGC GCTCTCTGCA GCTGAATCAA TGGATGATCA TTTATGGCCA 
GAGAGAGCAA GTCCATTTGC ATCGAGGCAT GGCTCATTGA AGAGAAGAAC GAGGGAACTT 
GATCCCGAGA AAAAGGGGGA CAAAACAAAT ATTGTTCAAG ATCTACGTGC AAGATTTGAA 
ATGAAATCTC CACCAAAATC ACCACCGAAA GATGATGAAG AAGGAACATC TACGGATCCC 
AAACATTTCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_504782.2
CDS	1..4635
Translation	MSNDNLAIEIDPPDDVEYDVEAGFNSSTCHLMHNFEEERPTSLVASIKESAISSKRKLKYYCSFICTPIKKLLSTFKLLIIIGLYSFIGAHIFMYLEVPTDLEAREDGFHQRKIAREVMVLNLRAIFYDNREDREERWKHAILKFEEDIGLEEPVIETVWTFWMSFLYAGTIFTTIGYGNIACKTRAGQIATMVYAFVGIPIMLVMLTSLNNFLLKWIKLITNGVSDMTLYIGVRLGITVIRQDEVQKRLRYTKLAKTMKRWKLSKHGAPSSIAISNSEENRLNSTPEDDEEEEEIHQDPPVLSTLIATVAWIILSAAVFCLFEDWTFFTSFYFCFISLTTIGLGDVTPANPEYMIATFGVVIVGLSMLTVCIDVLQEKLAQMYMALLQKLLTEYMEAVKSGDPNAASAMMAGFQGRAKFLMPLISKNEGAKVMDKFKQDCSKKGIDPPPVLTNINPETGMPAFANAPKEDFRDYIEVADEKFADEQQRLSQTPLSKSTDFLQSPTPRPTVTVAVTQTARPQACVSLEVQAGTTMGKFHDYGSQTQTESSDEGIQTDYSIDFDDEKNKEPEKVMADIGTQYEIVLLTSIGIQPEVSSIYIGAGLSDHSDEESGCESEDDSDDSDVSSDEESELKSSAKYKETPLPFEVEDPGMMSLGLQSPPIEITPFTDDESAASDARSSPVPPTSSKRSKLTPKTIKVGKSLSMDSTDTVIHVKPVGVNDDEKRSEEKESKDQETNDDLKTESGQEKKRRKKKKKLRSKKTREHELRFLKNSGNQASPLLMDCSTQYEIFTVESGSQYDVKGRSRKCQTKLSSINHEQLQGIRETVQKLRRRESEMSKVSNVSESDWDSETGKEPEVAEISLDLSVVALGVDESGKVVDIPKEVPKAVQRHESFSPTSELTTASESLSAEKVHLDSEDEELESLEELELYTTDAGIQTETKVMLDKQDNATPKLLEIIKNCSTQTTFSTFHIPQKVYESASTSSDEEDHKNDGEDEKQKKSKQEVFFMTNTGVQTMHTSFHIDEKPKFVEENLQTQNTHDGIKEQSAVDEEMPLFLKNSDCQTVEVISFECATSTENVSFVGDVETSEGSSQTAMQVSETEVQTMQQEFVVATMQTDEPLLVSDESQTEQTKSSEIDSQTDPPSTKSFICQTELITSSEVEVQAVRAVEFSLVECQTDALNHEEVEVQTELDISVSQSQTDNVDVGHIEMQTDMTITNASESQTDVLSVGDTEMQTDEVDSIIFKLIFVDVHEITFFRKLIRHSHTKFGPVVVLMLVEIFQSEKSTNFWKKEIFTIESQTEQPSTELQETQTDDVSFAGDVPTTDQITQSESAQVFDSETQYALSIEEKDVQATPISFETQTQHSIELEVKSCQTEGVRMKNKKSQVYVQELDEERRMRMSDIGIQTGVLARVQHIYTRPISDDVGPSSSAPITLRKSSDSSEHISTKTAKSSEESLAPSASGTALSAAESMDDHLWPERASPFASRHGSLKRRTRELDPEKKGDKTNIVQDLRARFEMKSPPKSPPKDDEEGTSTDPKHFF

Target ORF information:

RefSeq Version	NM_072381.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TWiK family of potassium channels (twk-11), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_072381.5

ORF Insert Sequence:

ATGTCCAATG ATAACTTGGC GATTGAAATC GACCCTCCGG ACGATGTGGA GTATGATGTG 
GAAGCAGGCT TCAACTCTTC CACATGCCAT TTAATGCACA ATTTCGAAGA GGAACGTCCA 
ACCTCTCTCG TAGCCTCCAT AAAGGAAAGT GCCATTAGCT CAAAGCGAAA ACTAAAATAC 
TACTGCTCGT TCATATGCAC CCCAATCAAG AAGTTGTTAT CCACTTTCAA ACTACTCATC 
ATAATCGGTC TTTACTCATT TATTGGCGCT CATATATTCA TGTACTTGGA AGTTCCAACT 
GATTTAGAAG CAAGAGAAGA TGGATTTCAT CAAAGAAAAA TAGCGAGGGA AGTGATGGTG 
CTGAATCTCC GTGCAATTTT CTACGACAAC CGCGAAGATC GCGAGGAACG ATGGAAGCAT 
GCCATTTTGA AGTTCGAAGA GGACATTGGC TTAGAAGAAC CTGTCATTGA AACTGTATGG 
ACTTTTTGGA TGTCTTTCCT CTATGCAGGA ACTATTTTCA CAACAATAGG ATATGGAAAC 
ATTGCATGTA AGACGCGTGC TGGCCAGATA GCTACAATGG TATATGCTTT TGTCGGAATT 
CCGATCATGC TGGTGATGCT CACCTCGCTC AACAATTTTT TGTTAAAATG GATAAAACTT 
ATTACGAATG GAGTTAGTGA TATGACGCTT TATATTGGTG TTCGCTTGGG AATAACAGTT 
ATTCGGCAGG ACGAAGTCCA GAAGCGCCTT CGCTACACAA AACTTGCCAA GACAATGAAA 
AGGTGGAAAC TTTCAAAACA CGGAGCTCCA AGTAGTATAG CGATTAGCAA TTCGGAAGAA 
AACCGGTTAA ATTCAACACC TGAAGACGAC GAAGAAGAGG AAGAAATTCA TCAGGATCCT 
CCAGTTCTTT CGACTCTAAT TGCAACTGTT GCCTGGATTA TTCTTTCGGC TGCTGTGTTT 
TGTCTTTTTG AGGACTGGAC ATTTTTCACC AGTTTTTATT TTTGTTTCAT CTCTCTGACA 
ACAATCGGTC TTGGAGATGT GACACCTGCT AATCCAGAAT ATATGATTGC AACATTTGGA 
GTTGTGATTG TCGGTTTATC AATGCTCACT GTCTGTATCG ACGTCTTACA AGAAAAACTT 
GCTCAAATGT ATATGGCTCT ACTTCAGAAG TTATTGACAG AGTACATGGA AGCTGTGAAA 
AGCGGAGACC CAAATGCTGC ATCAGCTATG ATGGCTGGTT TTCAAGGAAG GGCAAAGTTT 
TTGATGCCGT TGATTAGTAA AAACGAAGGA GCAAAAGTTA TGGATAAGTT CAAACAAGAT 
TGTTCAAAGA AGGGAATCGA TCCACCACCG GTTTTAACAA ATATCAACCC GGAGACCGGA 
ATGCCAGCTT TTGCAAATGC TCCGAAAGAA GATTTTAGAG ATTATATTGA AGTTGCAGAT 
GAAAAATTTG CTGATGAACA ACAGAGGCTC TCTCAAACCC CGTTATCAAA ATCCACCGAC 
TTTTTACAAT CACCCACACC ACGTCCAACT GTCACTGTAG CTGTTACTCA AACAGCTAGA 
CCACAGGCAT GCGTATCACT AGAAGTACAG GCTGGAACCA CAATGGGAAA GTTCCATGAT 
TATGGATCAC AGACACAGAC GGAAAGTTCT GACGAAGGAA TTCAGACGGA TTATAGCATT 
GATTTTGATG ATGAAAAAAA CAAGGAGCCA GAGAAAGTTA TGGCGGACAT TGGCACTCAA 
TATGAAATCG TTCTATTAAC AAGTATTGGA ATTCAACCAG AAGTCTCATC AATTTATATT 
GGAGCCGGCT TATCTGATCA TTCGGACGAA GAAAGTGGAT GTGAATCAGA AGACGATTCG 
GATGACTCGG ATGTATCATC CGATGAGGAA AGTGAACTTA AATCTTCTGC GAAATACAAA 
GAAACACCTC TTCCATTTGA AGTCGAAGAT CCAGGAATGA TGTCGCTCGG ATTACAGTCT 
CCACCAATTG AAATAACTCC CTTCACGGAT GATGAATCAG CTGCAAGCGA CGCAAGAAGT 
TCTCCTGTCC CACCAACATC TTCCAAGCGA TCCAAGTTGA CACCAAAGAC TATAAAAGTT 
GGCAAATCGT TATCAATGGA CTCGACTGAT ACAGTTATTC ATGTGAAACC AGTTGGCGTT 
AATGATGATG AGAAAAGAAG TGAAGAAAAA GAAAGTAAAG ATCAAGAAAC AAATGATGAC 
CTAAAAACTG AAAGTGGACA GGAGAAAAAG AGAAGAAAGA AAAAGAAGAA GCTTCGTTCA 
AAGAAGACAA GAGAGCATGA GCTGAGATTT TTGAAGAATA GCGGAAATCA AGCATCTCCC 
CTTCTTATGG ATTGCTCTAC ACAATATGAG ATATTTACAG TGGAAAGTGG GTCGCAATAC 
GATGTGAAAG GAAGAAGCCG AAAATGTCAG ACAAAACTTT CCAGTATAAA TCATGAGCAG 
TTACAAGGGA TTCGAGAAAC AGTTCAAAAG TTGAGAAGAA GAGAAAGTGA AATGAGTAAG 
GTGTCGAATG TTTCTGAAAG TGACTGGGAT AGTGAAACTG GAAAAGAACC GGAAGTCGCT 
GAAATCAGTT TAGATCTTTC TGTAGTTGCT CTCGGAGTTG ATGAATCCGG GAAAGTTGTT 
GATATTCCTA AGGAAGTACC TAAAGCTGTT CAACGACATG AATCATTTTC ACCAACATCG 
GAGCTCACCA CTGCGTCCGA ATCACTTTCC GCTGAGAAAG TACATCTGGA TTCAGAAGAT 
GAGGAGTTAG AAAGTCTTGA AGAACTCGAA CTGTATACCA CAGACGCCGG AATTCAGACT 
GAAACAAAGG TCATGCTGGA CAAGCAAGAT AATGCAACTC CAAAGTTGCT GGAAATTATT 
AAAAACTGCA GCACTCAGAC AACATTCAGC ACATTCCACA TTCCGCAGAA GGTATATGAG 
TCGGCGAGCA CCTCAAGTGA CGAGGAAGAT CATAAAAACG ATGGTGAAGA TGAAAAACAG 
AAGAAATCTA AACAGGAAGT GTTCTTTATG ACCAACACTG GAGTACAGAC GATGCATACA 
TCGTTCCATA TTGATGAAAA ACCGAAATTT GTTGAAGAAA ATCTTCAAAC TCAAAATACC 
CACGACGGCA TAAAAGAACA AAGTGCCGTT GATGAAGAAA TGCCTCTGTT CTTAAAAAAT 
TCGGATTGTC AAACCGTTGA AGTAATATCA TTCGAATGTG CCACATCAAC AGAGAATGTT 
AGTTTTGTTG GAGACGTTGA AACTTCTGAA GGATCCAGTC AGACAGCCAT GCAAGTTTCT 
GAAACTGAAG TACAAACAAT GCAACAAGAA TTTGTGGTAG CAACAATGCA AACCGACGAG 
CCACTGTTGG TATCTGATGA GTCACAGACT GAGCAAACAA AATCTTCGGA AATTGATTCT 
CAAACTGATC CACCATCCAC AAAATCATTT ATATGTCAGA CAGAATTGAT AACAAGCTCG 
GAAGTTGAAG TTCAAGCAGT TCGAGCTGTA GAGTTTTCAC TTGTCGAATG CCAAACAGAT 
GCGCTAAATC ATGAAGAAGT AGAAGTTCAA ACAGAACTGG ATATTTCGGT TTCGCAGTCC 
CAAACTGATA ATGTTGATGT AGGCCACATT GAAATGCAAA CTGATATGAC AATAACAAAT 
GCATCGGAAT CTCAAACCGA TGTGCTCAGT GTAGGGGACA CGGAGATGCA AACAGACGAG 
GTGGATTCTA TTATTTTCAA ACTGATTTTC GTGGATGTGC ACGAAATTAC ATTTTTTCGA 
AAATTGATAA GACATTCACA TACAAAATTT GGTCCGGTAG TTGTTCTTAT GTTAGTTGAG 
ATATTTCAAT CTGAGAAATC AACCAATTTT TGGAAAAAAG AAATATTCAC AATAGAATCG 
CAAACCGAAC AACCATCCAC AGAACTGCAA GAGACTCAAA CTGACGATGT TTCTTTTGCT 
GGCGATGTTC CAACAACCGA CCAAATCACA CAATCGGAAT CTGCTCAAGT TTTCGATTCT 
GAAACTCAAT ATGCTTTATC CATAGAAGAA AAGGACGTCC AGGCAACGCC AATCTCATTT 
GAAACTCAGA CACAACATTC CATTGAACTT GAAGTGAAAT CTTGCCAAAC CGAAGGAGTT 
CGAATGAAAA ACAAAAAGAG TCAGGTGTAT GTTCAAGAAC TCGATGAAGA ACGTCGAATG 
CGTATGTCAG ACATTGGCAT ACAAACCGGT GTTCTGGCAC GTGTTCAACA CATTTATACC 
CGTCCAATAT CTGATGATGT GGGACCATCT TCATCTGCTC CAATCACTCT TCGAAAGTCT 
TCCGACTCCT CGGAACACAT ATCAACGAAA ACTGCGAAAA GCAGCGAAGA GTCATTGGCG 
CCATCCGCTT CCGGGACAGC GCTCTCTGCA GCTGAATCAA TGGATGATCA TTTATGGCCA 
GAGAGAGCAA GTCCATTTGC ATCGAGGCAT GGCTCATTGA AGAGAAGAAC GAGGGAACTT 
GATCCCGAGA AAAAGGGGGA CAAAACAAAT ATTGTTCAAG ATCTACGTGC AAGATTTGAA 
ATGAAATCTC CACCAAAATC ACCACCGAAA GATGATGAAG AAGGAACATC TACGGATCCC 
AAACATTTCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twk-11 ( NM_072382.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_504783.2 Caenorhabditis elegans TWiK family of potassium channels (twk-11), partial mRNA.

Price & Availability↑

CloneID	OCa347227
Clone ID Related Accession (Same CDS sequence)	NM_072382.6 , NM_072382.7
Accession Version	NM_072382.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	TWiK family of potassium channels
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_072382.5. COMPLETENESS: incomplete on both ends.

ATGTCCAATG ATAACTTGGC GATTGAAATC GACCCTCCGG ACGATGTGGA GTATGATGTG 
GAAGCAGGCT TCAACTCTTC CACATGCCAT TTAATGCACA ATTTCGAAGA GGAACGTCCA 
ACCTCTCTCG TAGCCTCCAT AAAGGAAAGT GCCATTAGCT CAAAGCGAAA ACTAAAATAC 
TACTGCTCGT TCATATGCAC CCCAATCAAG AAGTTGTTAT CCACTTTCAA ACTACTCATC 
ATAATCGGTC TTTACTCATT TATTGGCGCT CATATATTCA TGTACTTGGA AGTTCCAACT 
GATTTAGAAG CAAGAGAAGA TGGATTTCAT CAAAGAAAAA TAGCGAGGGA AGTGATGGTG 
CTGAATCTCC GTGCAATTTT CTACGACAAC CGCGAAGATC GCGAGGAACG ATGGAAGCAT 
GCCATTTTGA AGTTCGAAGA GGACATTGGC TTAGAAGAAC CTGTCATTGA AACTGTATGG 
ACTTTTTGGA TGTCTTTCCT CTATGCAGGA ACTATTTTCA CAACAATAGG ATATGGAAAC 
ATTGCATGTA AGACGCGTGC TGGCCAGATA GCTACAATGG TATATGCTTT TGTCGGAATT 
CCGATCATGC TGGTGATGCT CACCTCGCTC AACAATTTTT TGTTAAAATG GATAAAACTT 
ATTACGAATG GAGTTAGTGA TATGACGCTT TATATTGGTG TTCGCTTGGG AATAACAGTT 
ATTCGGCAGG ACGAAGTCCA GAAGCGCCTT CGCTACACAA AACTTGCCAA GACAATGAAA 
AGGTGGAAAC TTTCAAAACA CGGAGCTCCA AGTAGTATAG CGATTAGCAA TTCGGAAGAA 
AACCGGTTAA ATTCAACACC TGAAGACGAC GAAGAAGAGG AAGAAATTCA TCAGGATCCT 
CCAGTTCTTT CGACTCTAAT TGCAACTGTT GCCTGGATTA TTCTTTCGGC TGCTGTGTTT 
TGTCTTTTTG AGGACTGGAC ATTTTTCACC AGTTTTTATT TTTGTTTCAT CTCTCTGACA 
ACAATCGGTC TTGGAGATGT GACACCTGCT AATCCAGAAT ATATGATTGC AACATTTGGA 
GTTGTGATTG TCGGTTTATC AATGCTCACT GTCTGTATCG ACGTCTTACA AGAAAAACTT 
GCTCAAATGT ATATGGCTCT ACTTCAGAAG TTATTGACAG AGTACATGGA AGCTGTGAAA 
AGCGGAGACC CAAATGCTGC ATCAGCTATG ATGGCTGGTT TTCAAGGAAG GGCAAAGTTT 
TTGATGCCGT TGATTAGTAA AAACGAAGGA GCAAAAGTTA TGGATAAGTT CAAACAAGAT 
TGTTCAAAGA AGGGAATCGA TCCACCACCG GTTTTAACAA ATATCAACCC GGAGACCGGA 
ATGCCAGCTT TTGCAAATGC TCCGAAAGAA GATTTTAGAG ATTATATTGA AGTTGCAGAT 
GAAAAATTTG CTGATGAACA ACAGAGGCTC TCTCAAACCC CGTTATCAAA ATCCACCGAC 
TTTTTACAAT CACCCACACC ACGTCCAACT GTCACTGTAG CTGTTACTCA AACAGCTAGA 
CCACAGGCAT GCGTATCACT AGAAGTACAG GCTGGAACCA CAATGGGAAA GTTCCATGAT 
TATGGATCAC AGACACAGAC GGAAAGTTCT GACGAAGGAA TTCAGACGGA TTATAGCATT 
GATTTTGATG ATGAAAAAAA CAAGGAGCCA GAGAAAGTTA TGGCGGACAT TGGCACTCAA 
TATGAAATCG TTCTATTAAC AAGTATTGGA ATTCAACCAG AAGTCTCATC AATTTATATT 
GGAGCCGGCT TATCTGATCA TTCGGACGAA GAAAGTGGAT GTGAATCAGA AGACGATTCG 
GATGACTCGG ATGTATCATC CGATGAGGAA AGTGAACTTA AATCTTCTGC GAAATACAAA 
GAAACACCTC TTCCATTTGA AGTCGAAGAT CCAGGAATGA TGTCGCTCGG ATTACAGTCT 
CCACCAATTG AAATAACTCC CTTCACGGAT GATGAATCAG CTGCAAGCGA CGCAAGAAGT 
TCTCCTGTCC CACCAACATC TTCCAAGCGA TCCAAGTTGA CACCAAAGAC TATAAAAGTT 
GGCAAATCGT TATCAATGGA CTCGACTGAT ACAGTTATTC ATGTGAAACC AGTTGGCGTT 
AATGATGATG AGAAAAGAAG TGAAGAAAAA GAAAGTAAAG ATCAAGAAAC AAATGATGAC 
CTAAAAACTG AAAGTGGACA GGAGAAAAAG AGAAGAAAGA AAAAGAAGAA GCTTCGTTCA 
AAGAAGACAA GAGAGCATGA GCTGAGATTT TTGAAGAATA GCGGAAATCA AGCATCTCCC 
CTTCTTATGG ATTGCTCTAC ACAATATGAG ATATTTACAG TGGAAAGTGG GTCGCAATAC 
GATGTGAAAG GAAGAAGCCG AAAATGTCAG ACAAAACTTT CCAGTATAAA TCATGAGCAG 
TTACAAGGGA TTCGAGAAAC AGTTCAAAAG TTGAGAAGAA GAGAAAGTGA AATGAGTAAG 
GTGTCGAATG TTTCTGAAAG TGACTGGGAT AGTGAAACTG GAAAAGAACC GGAAGTCGCT 
GAAATCAGTT TAGATCTTTC TGTAGTTGCT CTCGGAGTTG ATGAATCCGG GAAAGTTGTT 
GATATTCCTA AGGAAGTACC TAAAGCTGTT CAACGACATG AATCATTTTC ACCAACATCG 
GAGCTCACCA CTGCGTCCGA ATCACTTTCC GCTGAGAAAG TACATCTGGA TTCAGAAGAT 
GAGGAGTTAG AAAGTCTTGA AGAACTCGAA CTGTATACCA CAGACGCCGG AATTCAGACT 
GAAACAAAGG TCATGCTGGA CAAGCAAGAT AATGCAACTC CAAAGTTGCT GGAAATTATT 
AAAAACTGCA GCACTCAGAC AACATTCAGC ACATTCCACA TTCCGCAGAA GGTATATGAG 
TCGGCGAGCA CCTCAAGTGA CGAGGAAGAT CATAAAAACG ATGGTGAAGA TGAAAAACAG 
AAGAAATCTA AACAGGAAGT GTTCTTTATG ACCAACACTG GAGTACAGAC GATGCATACA 
TCGTTCCATA TTGATGAAAA ACCGAAATTT GTTGAAGAAA ATCTTCAAAC TCAAAATACC 
CACGACGGCA TAAAAGAACA AAGTGCCGTT GATGAAGAAA TGCCTCTGTT CTTAAAAAAT 
TCGGATTGTC AAACCGTTGA AGTAATATCA TTCGAATGTG CCACATCAAC AGAGAATGTT 
AGTTTTGTTG GAGACGTTGA AACTTCTGAA GGATCCAGTC AGACAGCCAT GCAAGTTTCT 
GAAACTGAAG TACAAACAAT GCAACAAGAA TTTGTGGTAG CAACAATGCA AACCGACGAG 
CCACTGTTGG TATCTGATGA GTCACAGACT GAGCAAACAA AATCTTCGGA AATTGATTCT 
CAAACTGATC CACCATCCAC AAAATCATTT ATATGTCAGA CAGAATTGAT AACAAGCTCG 
GAAGTTGAAG TTCAAGCAGT TCGAGCTGTA GAGTTTTCAC TTGTCGAATG CCAAACAGAT 
GCGCTAAATC ATGAAGAAGT AGAAGTTCAA ACAGAACTGG ATATTTCGGT TTCGCAGTCC 
CAAACTGATA ATGTTGATGT AGGCCACATT GAAATGCAAA CTGATATGAC AATAACAAAT 
GCATCGGAAT CTCAAACCGA TGTGCTCAGT GTAGGGGACA CGGAGATGCA AACAGACGAG 
AAAGAAATAT TCACAATAGA ATCGCAAACC GAACAACCAT CCACAGAACT GCAAGAGACT 
CAAACTGACG ATGTTTCTTT TGCTGGCGAT GTTCCAACAA CCGACCAAAT CACACAATCG 
GAATCTGCTC AAGTTTTCGA TTCTGAAACT CAATATGCTT TATCCATAGA AGAAAAGGAC 
GTCCAGGCAA CGCCAATCTC ATTTGAAACT CAGACACAAC ATTCCATTGA ACTTGAAGTG 
AAATCTTGCC AAACCGAAGG AGTTCGAATG AAAAACAAAA AGAGTCAGGT AGAAGACGAC 
TCAATGCTGT CAGTTCTCAA AATAGATAGT GAAAGTCAAA TAGAAGTGGA CGTTTCGTCC 
ATTAATCTTT CTCCAATTAG ATTAGATGTC TCCAATATGT TCACAATGAC AGAGCCAGGA 
GCTTCTGAAG AGAAAGAAGT ACAAGTTGAT GAGGACTGTT TTGAACCGGA GGAAACTTCA 
CGTAATTATT ATGCAGGACC GCTTTGGAGT GATTTTGATT ATACGCCGGT GATAGGCGAA 
GATGGGGAAG AAGTTATGGA CGGAGAGACT CAGACACAGG ATGGAAGCGA GCCAGGTGAT 
CCAGAATTTG ATGAACAGAG AGGACAGGAA CAAGAAGAAG GTGAAGATGA TAAAGAAAGA 
GCTCATGATC AAACTGCTAT CGGTAGATCA TCTCGTAGAA AGAAGATTAG AAAAAGTCAA 
TGCATCTCAT CAGAAAACTT GCTGGAAACA CCTTCAAGTG TCCAACAATC ATCAGATAGT 
CTTGCTTCTG AATCAGCAGT TCAATGTTCC CCCGAATCAT ATGATATGAA AATCCAAACG 
TCCAAGTTTA AAAAGAAAAA GAAGGTTGAG AAGAACATAG AAGCCAAGGT TGAAATGAGC 
ACTTCAACTG TGCAAGCAAC CACTGAAATG TGCGAGCAAA GTGCTGGAAG CATTGAAGAC 
GACTGGCTTC AGGTGTATGT TCAAGAACTC GATGAAGAAC GTCGAATGCG TATGTCAGAC 
ATTGGCATAC AAACCGGTGT TCTGGCACGT GTTCAACACA TTTATACCCG TCCAATATCT 
GATGATGTGG GACCATCTTC ATCTGCTCCA ATCACTCTTC GAAAGTCTTC CGACTCCTCG 
GAACACATAT CAACGAAAAC TGCGAAAAGC AGCGAAGAGT CATTGGCGCC ATCCGCTTCC 
GGGACAGCGC TCTCTGCAGC TGAATCAATG GATGATCATT TATGGCCAGA GAGAGCAAGT 
CCATTTGCAT CGAGGCATGG CTCATTGAAG AGAAGAACGA GGGAACTTGA TCCCGAGAAA 
AAGGGGGACA AAACAAATAT TGTTCAAGAT CTACGTGCAA GATTTGAAAT GAAATCTCCA 
CCAAAATCAC CACCGAAAGA TGATGAAGAA GGAACATCTA CGGATCCCAA ACATTTCTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_504783.2
CDS	1..5163
Translation	MSNDNLAIEIDPPDDVEYDVEAGFNSSTCHLMHNFEEERPTSLVASIKESAISSKRKLKYYCSFICTPIKKLLSTFKLLIIIGLYSFIGAHIFMYLEVPTDLEAREDGFHQRKIAREVMVLNLRAIFYDNREDREERWKHAILKFEEDIGLEEPVIETVWTFWMSFLYAGTIFTTIGYGNIACKTRAGQIATMVYAFVGIPIMLVMLTSLNNFLLKWIKLITNGVSDMTLYIGVRLGITVIRQDEVQKRLRYTKLAKTMKRWKLSKHGAPSSIAISNSEENRLNSTPEDDEEEEEIHQDPPVLSTLIATVAWIILSAAVFCLFEDWTFFTSFYFCFISLTTIGLGDVTPANPEYMIATFGVVIVGLSMLTVCIDVLQEKLAQMYMALLQKLLTEYMEAVKSGDPNAASAMMAGFQGRAKFLMPLISKNEGAKVMDKFKQDCSKKGIDPPPVLTNINPETGMPAFANAPKEDFRDYIEVADEKFADEQQRLSQTPLSKSTDFLQSPTPRPTVTVAVTQTARPQACVSLEVQAGTTMGKFHDYGSQTQTESSDEGIQTDYSIDFDDEKNKEPEKVMADIGTQYEIVLLTSIGIQPEVSSIYIGAGLSDHSDEESGCESEDDSDDSDVSSDEESELKSSAKYKETPLPFEVEDPGMMSLGLQSPPIEITPFTDDESAASDARSSPVPPTSSKRSKLTPKTIKVGKSLSMDSTDTVIHVKPVGVNDDEKRSEEKESKDQETNDDLKTESGQEKKRRKKKKKLRSKKTREHELRFLKNSGNQASPLLMDCSTQYEIFTVESGSQYDVKGRSRKCQTKLSSINHEQLQGIRETVQKLRRRESEMSKVSNVSESDWDSETGKEPEVAEISLDLSVVALGVDESGKVVDIPKEVPKAVQRHESFSPTSELTTASESLSAEKVHLDSEDEELESLEELELYTTDAGIQTETKVMLDKQDNATPKLLEIIKNCSTQTTFSTFHIPQKVYESASTSSDEEDHKNDGEDEKQKKSKQEVFFMTNTGVQTMHTSFHIDEKPKFVEENLQTQNTHDGIKEQSAVDEEMPLFLKNSDCQTVEVISFECATSTENVSFVGDVETSEGSSQTAMQVSETEVQTMQQEFVVATMQTDEPLLVSDESQTEQTKSSEIDSQTDPPSTKSFICQTELITSSEVEVQAVRAVEFSLVECQTDALNHEEVEVQTELDISVSQSQTDNVDVGHIEMQTDMTITNASESQTDVLSVGDTEMQTDEKEIFTIESQTEQPSTELQETQTDDVSFAGDVPTTDQITQSESAQVFDSETQYALSIEEKDVQATPISFETQTQHSIELEVKSCQTEGVRMKNKKSQVEDDSMLSVLKIDSESQIEVDVSSINLSPIRLDVSNMFTMTEPGASEEKEVQVDEDCFEPEETSRNYYAGPLWSDFDYTPVIGEDGEEVMDGETQTQDGSEPGDPEFDEQRGQEQEEGEDDKERAHDQTAIGRSSRRKKIRKSQCISSENLLETPSSVQQSSDSLASESAVQCSPESYDMKIQTSKFKKKKKVEKNIEAKVEMSTSTVQATTEMCEQSAGSIEDDWLQVYVQELDEERRMRMSDIGIQTGVLARVQHIYTRPISDDVGPSSSAPITLRKSSDSSEHISTKTAKSSEESLAPSASGTALSAAESMDDHLWPERASPFASRHGSLKRRTRELDPEKKGDKTNIVQDLRARFEMKSPPKSPPKDDEEGTSTDPKHFF

Target ORF information:

RefSeq Version	NM_072382.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TWiK family of potassium channels (twk-11), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_072382.6

ORF Insert Sequence:

ATGTCCAATG ATAACTTGGC GATTGAAATC GACCCTCCGG ACGATGTGGA GTATGATGTG 
GAAGCAGGCT TCAACTCTTC CACATGCCAT TTAATGCACA ATTTCGAAGA GGAACGTCCA 
ACCTCTCTCG TAGCCTCCAT AAAGGAAAGT GCCATTAGCT CAAAGCGAAA ACTAAAATAC 
TACTGCTCGT TCATATGCAC CCCAATCAAG AAGTTGTTAT CCACTTTCAA ACTACTCATC 
ATAATCGGTC TTTACTCATT TATTGGCGCT CATATATTCA TGTACTTGGA AGTTCCAACT 
GATTTAGAAG CAAGAGAAGA TGGATTTCAT CAAAGAAAAA TAGCGAGGGA AGTGATGGTG 
CTGAATCTCC GTGCAATTTT CTACGACAAC CGCGAAGATC GCGAGGAACG ATGGAAGCAT 
GCCATTTTGA AGTTCGAAGA GGACATTGGC TTAGAAGAAC CTGTCATTGA AACTGTATGG 
ACTTTTTGGA TGTCTTTCCT CTATGCAGGA ACTATTTTCA CAACAATAGG ATATGGAAAC 
ATTGCATGTA AGACGCGTGC TGGCCAGATA GCTACAATGG TATATGCTTT TGTCGGAATT 
CCGATCATGC TGGTGATGCT CACCTCGCTC AACAATTTTT TGTTAAAATG GATAAAACTT 
ATTACGAATG GAGTTAGTGA TATGACGCTT TATATTGGTG TTCGCTTGGG AATAACAGTT 
ATTCGGCAGG ACGAAGTCCA GAAGCGCCTT CGCTACACAA AACTTGCCAA GACAATGAAA 
AGGTGGAAAC TTTCAAAACA CGGAGCTCCA AGTAGTATAG CGATTAGCAA TTCGGAAGAA 
AACCGGTTAA ATTCAACACC TGAAGACGAC GAAGAAGAGG AAGAAATTCA TCAGGATCCT 
CCAGTTCTTT CGACTCTAAT TGCAACTGTT GCCTGGATTA TTCTTTCGGC TGCTGTGTTT 
TGTCTTTTTG AGGACTGGAC ATTTTTCACC AGTTTTTATT TTTGTTTCAT CTCTCTGACA 
ACAATCGGTC TTGGAGATGT GACACCTGCT AATCCAGAAT ATATGATTGC AACATTTGGA 
GTTGTGATTG TCGGTTTATC AATGCTCACT GTCTGTATCG ACGTCTTACA AGAAAAACTT 
GCTCAAATGT ATATGGCTCT ACTTCAGAAG TTATTGACAG AGTACATGGA AGCTGTGAAA 
AGCGGAGACC CAAATGCTGC ATCAGCTATG ATGGCTGGTT TTCAAGGAAG GGCAAAGTTT 
TTGATGCCGT TGATTAGTAA AAACGAAGGA GCAAAAGTTA TGGATAAGTT CAAACAAGAT 
TGTTCAAAGA AGGGAATCGA TCCACCACCG GTTTTAACAA ATATCAACCC GGAGACCGGA 
ATGCCAGCTT TTGCAAATGC TCCGAAAGAA GATTTTAGAG ATTATATTGA AGTTGCAGAT 
GAAAAATTTG CTGATGAACA ACAGAGGCTC TCTCAAACCC CGTTATCAAA ATCCACCGAC 
TTTTTACAAT CACCCACACC ACGTCCAACT GTCACTGTAG CTGTTACTCA AACAGCTAGA 
CCACAGGCAT GCGTATCACT AGAAGTACAG GCTGGAACCA CAATGGGAAA GTTCCATGAT 
TATGGATCAC AGACACAGAC GGAAAGTTCT GACGAAGGAA TTCAGACGGA TTATAGCATT 
GATTTTGATG ATGAAAAAAA CAAGGAGCCA GAGAAAGTTA TGGCGGACAT TGGCACTCAA 
TATGAAATCG TTCTATTAAC AAGTATTGGA ATTCAACCAG AAGTCTCATC AATTTATATT 
GGAGCCGGCT TATCTGATCA TTCGGACGAA GAAAGTGGAT GTGAATCAGA AGACGATTCG 
GATGACTCGG ATGTATCATC CGATGAGGAA AGTGAACTTA AATCTTCTGC GAAATACAAA 
GAAACACCTC TTCCATTTGA AGTCGAAGAT CCAGGAATGA TGTCGCTCGG ATTACAGTCT 
CCACCAATTG AAATAACTCC CTTCACGGAT GATGAATCAG CTGCAAGCGA CGCAAGAAGT 
TCTCCTGTCC CACCAACATC TTCCAAGCGA TCCAAGTTGA CACCAAAGAC TATAAAAGTT 
GGCAAATCGT TATCAATGGA CTCGACTGAT ACAGTTATTC ATGTGAAACC AGTTGGCGTT 
AATGATGATG AGAAAAGAAG TGAAGAAAAA GAAAGTAAAG ATCAAGAAAC AAATGATGAC 
CTAAAAACTG AAAGTGGACA GGAGAAAAAG AGAAGAAAGA AAAAGAAGAA GCTTCGTTCA 
AAGAAGACAA GAGAGCATGA GCTGAGATTT TTGAAGAATA GCGGAAATCA AGCATCTCCC 
CTTCTTATGG ATTGCTCTAC ACAATATGAG ATATTTACAG TGGAAAGTGG GTCGCAATAC 
GATGTGAAAG GAAGAAGCCG AAAATGTCAG ACAAAACTTT CCAGTATAAA TCATGAGCAG 
TTACAAGGGA TTCGAGAAAC AGTTCAAAAG TTGAGAAGAA GAGAAAGTGA AATGAGTAAG 
GTGTCGAATG TTTCTGAAAG TGACTGGGAT AGTGAAACTG GAAAAGAACC GGAAGTCGCT 
GAAATCAGTT TAGATCTTTC TGTAGTTGCT CTCGGAGTTG ATGAATCCGG GAAAGTTGTT 
GATATTCCTA AGGAAGTACC TAAAGCTGTT CAACGACATG AATCATTTTC ACCAACATCG 
GAGCTCACCA CTGCGTCCGA ATCACTTTCC GCTGAGAAAG TACATCTGGA TTCAGAAGAT 
GAGGAGTTAG AAAGTCTTGA AGAACTCGAA CTGTATACCA CAGACGCCGG AATTCAGACT 
GAAACAAAGG TCATGCTGGA CAAGCAAGAT AATGCAACTC CAAAGTTGCT GGAAATTATT 
AAAAACTGCA GCACTCAGAC AACATTCAGC ACATTCCACA TTCCGCAGAA GGTATATGAG 
TCGGCGAGCA CCTCAAGTGA CGAGGAAGAT CATAAAAACG ATGGTGAAGA TGAAAAACAG 
AAGAAATCTA AACAGGAAGT GTTCTTTATG ACCAACACTG GAGTACAGAC GATGCATACA 
TCGTTCCATA TTGATGAAAA ACCGAAATTT GTTGAAGAAA ATCTTCAAAC TCAAAATACC 
CACGACGGCA TAAAAGAACA AAGTGCCGTT GATGAAGAAA TGCCTCTGTT CTTAAAAAAT 
TCGGATTGTC AAACCGTTGA AGTAATATCA TTCGAATGTG CCACATCAAC AGAGAATGTT 
AGTTTTGTTG GAGACGTTGA AACTTCTGAA GGATCCAGTC AGACAGCCAT GCAAGTTTCT 
GAAACTGAAG TACAAACAAT GCAACAAGAA TTTGTGGTAG CAACAATGCA AACCGACGAG 
CCACTGTTGG TATCTGATGA GTCACAGACT GAGCAAACAA AATCTTCGGA AATTGATTCT 
CAAACTGATC CACCATCCAC AAAATCATTT ATATGTCAGA CAGAATTGAT AACAAGCTCG 
GAAGTTGAAG TTCAAGCAGT TCGAGCTGTA GAGTTTTCAC TTGTCGAATG CCAAACAGAT 
GCGCTAAATC ATGAAGAAGT AGAAGTTCAA ACAGAACTGG ATATTTCGGT TTCGCAGTCC 
CAAACTGATA ATGTTGATGT AGGCCACATT GAAATGCAAA CTGATATGAC AATAACAAAT 
GCATCGGAAT CTCAAACCGA TGTGCTCAGT GTAGGGGACA CGGAGATGCA AACAGACGAG 
AAAGAAATAT TCACAATAGA ATCGCAAACC GAACAACCAT CCACAGAACT GCAAGAGACT 
CAAACTGACG ATGTTTCTTT TGCTGGCGAT GTTCCAACAA CCGACCAAAT CACACAATCG 
GAATCTGCTC AAGTTTTCGA TTCTGAAACT CAATATGCTT TATCCATAGA AGAAAAGGAC 
GTCCAGGCAA CGCCAATCTC ATTTGAAACT CAGACACAAC ATTCCATTGA ACTTGAAGTG 
AAATCTTGCC AAACCGAAGG AGTTCGAATG AAAAACAAAA AGAGTCAGGT AGAAGACGAC 
TCAATGCTGT CAGTTCTCAA AATAGATAGT GAAAGTCAAA TAGAAGTGGA CGTTTCGTCC 
ATTAATCTTT CTCCAATTAG ATTAGATGTC TCCAATATGT TCACAATGAC AGAGCCAGGA 
GCTTCTGAAG AGAAAGAAGT ACAAGTTGAT GAGGACTGTT TTGAACCGGA GGAAACTTCA 
CGTAATTATT ATGCAGGACC GCTTTGGAGT GATTTTGATT ATACGCCGGT GATAGGCGAA 
GATGGGGAAG AAGTTATGGA CGGAGAGACT CAGACACAGG ATGGAAGCGA GCCAGGTGAT 
CCAGAATTTG ATGAACAGAG AGGACAGGAA CAAGAAGAAG GTGAAGATGA TAAAGAAAGA 
GCTCATGATC AAACTGCTAT CGGTAGATCA TCTCGTAGAA AGAAGATTAG AAAAAGTCAA 
TGCATCTCAT CAGAAAACTT GCTGGAAACA CCTTCAAGTG TCCAACAATC ATCAGATAGT 
CTTGCTTCTG AATCAGCAGT TCAATGTTCC CCCGAATCAT ATGATATGAA AATCCAAACG 
TCCAAGTTTA AAAAGAAAAA GAAGGTTGAG AAGAACATAG AAGCCAAGGT TGAAATGAGC 
ACTTCAACTG TGCAAGCAAC CACTGAAATG TGCGAGCAAA GTGCTGGAAG CATTGAAGAC 
GACTGGCTTC AGGTGTATGT TCAAGAACTC GATGAAGAAC GTCGAATGCG TATGTCAGAC 
ATTGGCATAC AAACCGGTGT TCTGGCACGT GTTCAACACA TTTATACCCG TCCAATATCT 
GATGATGTGG GACCATCTTC ATCTGCTCCA ATCACTCTTC GAAAGTCTTC CGACTCCTCG 
GAACACATAT CAACGAAAAC TGCGAAAAGC AGCGAAGAGT CATTGGCGCC ATCCGCTTCC 
GGGACAGCGC TCTCTGCAGC TGAATCAATG GATGATCATT TATGGCCAGA GAGAGCAAGT 
CCATTTGCAT CGAGGCATGG CTCATTGAAG AGAAGAACGA GGGAACTTGA TCCCGAGAAA 
AAGGGGGACA AAACAAATAT TGTTCAAGAT CTACGTGCAA GATTTGAAAT GAAATCTCCA 
CCAAAATCAC CACCGAAAGA TGATGAAGAA GGAACATCTA CGGATCCCAA ACATTTCTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twk-11 ( NM_072382.7 ) cDNA ORF clone, Caenorhabditis elegans -> NP_504783.2 Caenorhabditis elegans TWiK family of potassium channels (twk-11), mRNA.

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CloneID	OCa347227
Clone ID Related Accession (Same CDS sequence)	NM_072382.6 , NM_072382.7
Accession Version	NM_072382.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	TWiK family of potassium channels
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_072382.6.

ATGTCCAATG ATAACTTGGC GATTGAAATC GACCCTCCGG ACGATGTGGA GTATGATGTG 
GAAGCAGGCT TCAACTCTTC CACATGCCAT TTAATGCACA ATTTCGAAGA GGAACGTCCA 
ACCTCTCTCG TAGCCTCCAT AAAGGAAAGT GCCATTAGCT CAAAGCGAAA ACTAAAATAC 
TACTGCTCGT TCATATGCAC CCCAATCAAG AAGTTGTTAT CCACTTTCAA ACTACTCATC 
ATAATCGGTC TTTACTCATT TATTGGCGCT CATATATTCA TGTACTTGGA AGTTCCAACT 
GATTTAGAAG CAAGAGAAGA TGGATTTCAT CAAAGAAAAA TAGCGAGGGA AGTGATGGTG 
CTGAATCTCC GTGCAATTTT CTACGACAAC CGCGAAGATC GCGAGGAACG ATGGAAGCAT 
GCCATTTTGA AGTTCGAAGA GGACATTGGC TTAGAAGAAC CTGTCATTGA AACTGTATGG 
ACTTTTTGGA TGTCTTTCCT CTATGCAGGA ACTATTTTCA CAACAATAGG ATATGGAAAC 
ATTGCATGTA AGACGCGTGC TGGCCAGATA GCTACAATGG TATATGCTTT TGTCGGAATT 
CCGATCATGC TGGTGATGCT CACCTCGCTC AACAATTTTT TGTTAAAATG GATAAAACTT 
ATTACGAATG GAGTTAGTGA TATGACGCTT TATATTGGTG TTCGCTTGGG AATAACAGTT 
ATTCGGCAGG ACGAAGTCCA GAAGCGCCTT CGCTACACAA AACTTGCCAA GACAATGAAA 
AGGTGGAAAC TTTCAAAACA CGGAGCTCCA AGTAGTATAG CGATTAGCAA TTCGGAAGAA 
AACCGGTTAA ATTCAACACC TGAAGACGAC GAAGAAGAGG AAGAAATTCA TCAGGATCCT 
CCAGTTCTTT CGACTCTAAT TGCAACTGTT GCCTGGATTA TTCTTTCGGC TGCTGTGTTT 
TGTCTTTTTG AGGACTGGAC ATTTTTCACC AGTTTTTATT TTTGTTTCAT CTCTCTGACA 
ACAATCGGTC TTGGAGATGT GACACCTGCT AATCCAGAAT ATATGATTGC AACATTTGGA 
GTTGTGATTG TCGGTTTATC AATGCTCACT GTCTGTATCG ACGTCTTACA AGAAAAACTT 
GCTCAAATGT ATATGGCTCT ACTTCAGAAG TTATTGACAG AGTACATGGA AGCTGTGAAA 
AGCGGAGACC CAAATGCTGC ATCAGCTATG ATGGCTGGTT TTCAAGGAAG GGCAAAGTTT 
TTGATGCCGT TGATTAGTAA AAACGAAGGA GCAAAAGTTA TGGATAAGTT CAAACAAGAT 
TGTTCAAAGA AGGGAATCGA TCCACCACCG GTTTTAACAA ATATCAACCC GGAGACCGGA 
ATGCCAGCTT TTGCAAATGC TCCGAAAGAA GATTTTAGAG ATTATATTGA AGTTGCAGAT 
GAAAAATTTG CTGATGAACA ACAGAGGCTC TCTCAAACCC CGTTATCAAA ATCCACCGAC 
TTTTTACAAT CACCCACACC ACGTCCAACT GTCACTGTAG CTGTTACTCA AACAGCTAGA 
CCACAGGCAT GCGTATCACT AGAAGTACAG GCTGGAACCA CAATGGGAAA GTTCCATGAT 
TATGGATCAC AGACACAGAC GGAAAGTTCT GACGAAGGAA TTCAGACGGA TTATAGCATT 
GATTTTGATG ATGAAAAAAA CAAGGAGCCA GAGAAAGTTA TGGCGGACAT TGGCACTCAA 
TATGAAATCG TTCTATTAAC AAGTATTGGA ATTCAACCAG AAGTCTCATC AATTTATATT 
GGAGCCGGCT TATCTGATCA TTCGGACGAA GAAAGTGGAT GTGAATCAGA AGACGATTCG 
GATGACTCGG ATGTATCATC CGATGAGGAA AGTGAACTTA AATCTTCTGC GAAATACAAA 
GAAACACCTC TTCCATTTGA AGTCGAAGAT CCAGGAATGA TGTCGCTCGG ATTACAGTCT 
CCACCAATTG AAATAACTCC CTTCACGGAT GATGAATCAG CTGCAAGCGA CGCAAGAAGT 
TCTCCTGTCC CACCAACATC TTCCAAGCGA TCCAAGTTGA CACCAAAGAC TATAAAAGTT 
GGCAAATCGT TATCAATGGA CTCGACTGAT ACAGTTATTC ATGTGAAACC AGTTGGCGTT 
AATGATGATG AGAAAAGAAG TGAAGAAAAA GAAAGTAAAG ATCAAGAAAC AAATGATGAC 
CTAAAAACTG AAAGTGGACA GGAGAAAAAG AGAAGAAAGA AAAAGAAGAA GCTTCGTTCA 
AAGAAGACAA GAGAGCATGA GCTGAGATTT TTGAAGAATA GCGGAAATCA AGCATCTCCC 
CTTCTTATGG ATTGCTCTAC ACAATATGAG ATATTTACAG TGGAAAGTGG GTCGCAATAC 
GATGTGAAAG GAAGAAGCCG AAAATGTCAG ACAAAACTTT CCAGTATAAA TCATGAGCAG 
TTACAAGGGA TTCGAGAAAC AGTTCAAAAG TTGAGAAGAA GAGAAAGTGA AATGAGTAAG 
GTGTCGAATG TTTCTGAAAG TGACTGGGAT AGTGAAACTG GAAAAGAACC GGAAGTCGCT 
GAAATCAGTT TAGATCTTTC TGTAGTTGCT CTCGGAGTTG ATGAATCCGG GAAAGTTGTT 
GATATTCCTA AGGAAGTACC TAAAGCTGTT CAACGACATG AATCATTTTC ACCAACATCG 
GAGCTCACCA CTGCGTCCGA ATCACTTTCC GCTGAGAAAG TACATCTGGA TTCAGAAGAT 
GAGGAGTTAG AAAGTCTTGA AGAACTCGAA CTGTATACCA CAGACGCCGG AATTCAGACT 
GAAACAAAGG TCATGCTGGA CAAGCAAGAT AATGCAACTC CAAAGTTGCT GGAAATTATT 
AAAAACTGCA GCACTCAGAC AACATTCAGC ACATTCCACA TTCCGCAGAA GGTATATGAG 
TCGGCGAGCA CCTCAAGTGA CGAGGAAGAT CATAAAAACG ATGGTGAAGA TGAAAAACAG 
AAGAAATCTA AACAGGAAGT GTTCTTTATG ACCAACACTG GAGTACAGAC GATGCATACA 
TCGTTCCATA TTGATGAAAA ACCGAAATTT GTTGAAGAAA ATCTTCAAAC TCAAAATACC 
CACGACGGCA TAAAAGAACA AAGTGCCGTT GATGAAGAAA TGCCTCTGTT CTTAAAAAAT 
TCGGATTGTC AAACCGTTGA AGTAATATCA TTCGAATGTG CCACATCAAC AGAGAATGTT 
AGTTTTGTTG GAGACGTTGA AACTTCTGAA GGATCCAGTC AGACAGCCAT GCAAGTTTCT 
GAAACTGAAG TACAAACAAT GCAACAAGAA TTTGTGGTAG CAACAATGCA AACCGACGAG 
CCACTGTTGG TATCTGATGA GTCACAGACT GAGCAAACAA AATCTTCGGA AATTGATTCT 
CAAACTGATC CACCATCCAC AAAATCATTT ATATGTCAGA CAGAATTGAT AACAAGCTCG 
GAAGTTGAAG TTCAAGCAGT TCGAGCTGTA GAGTTTTCAC TTGTCGAATG CCAAACAGAT 
GCGCTAAATC ATGAAGAAGT AGAAGTTCAA ACAGAACTGG ATATTTCGGT TTCGCAGTCC 
CAAACTGATA ATGTTGATGT AGGCCACATT GAAATGCAAA CTGATATGAC AATAACAAAT 
GCATCGGAAT CTCAAACCGA TGTGCTCAGT GTAGGGGACA CGGAGATGCA AACAGACGAG 
AAAGAAATAT TCACAATAGA ATCGCAAACC GAACAACCAT CCACAGAACT GCAAGAGACT 
CAAACTGACG ATGTTTCTTT TGCTGGCGAT GTTCCAACAA CCGACCAAAT CACACAATCG 
GAATCTGCTC AAGTTTTCGA TTCTGAAACT CAATATGCTT TATCCATAGA AGAAAAGGAC 
GTCCAGGCAA CGCCAATCTC ATTTGAAACT CAGACACAAC ATTCCATTGA ACTTGAAGTG 
AAATCTTGCC AAACCGAAGG AGTTCGAATG AAAAACAAAA AGAGTCAGGT AGAAGACGAC 
TCAATGCTGT CAGTTCTCAA AATAGATAGT GAAAGTCAAA TAGAAGTGGA CGTTTCGTCC 
ATTAATCTTT CTCCAATTAG ATTAGATGTC TCCAATATGT TCACAATGAC AGAGCCAGGA 
GCTTCTGAAG AGAAAGAAGT ACAAGTTGAT GAGGACTGTT TTGAACCGGA GGAAACTTCA 
CGTAATTATT ATGCAGGACC GCTTTGGAGT GATTTTGATT ATACGCCGGT GATAGGCGAA 
GATGGGGAAG AAGTTATGGA CGGAGAGACT CAGACACAGG ATGGAAGCGA GCCAGGTGAT 
CCAGAATTTG ATGAACAGAG AGGACAGGAA CAAGAAGAAG GTGAAGATGA TAAAGAAAGA 
GCTCATGATC AAACTGCTAT CGGTAGATCA TCTCGTAGAA AGAAGATTAG AAAAAGTCAA 
TGCATCTCAT CAGAAAACTT GCTGGAAACA CCTTCAAGTG TCCAACAATC ATCAGATAGT 
CTTGCTTCTG AATCAGCAGT TCAATGTTCC CCCGAATCAT ATGATATGAA AATCCAAACG 
TCCAAGTTTA AAAAGAAAAA GAAGGTTGAG AAGAACATAG AAGCCAAGGT TGAAATGAGC 
ACTTCAACTG TGCAAGCAAC CACTGAAATG TGCGAGCAAA GTGCTGGAAG CATTGAAGAC 
GACTGGCTTC AGGTGTATGT TCAAGAACTC GATGAAGAAC GTCGAATGCG TATGTCAGAC 
ATTGGCATAC AAACCGGTGT TCTGGCACGT GTTCAACACA TTTATACCCG TCCAATATCT 
GATGATGTGG GACCATCTTC ATCTGCTCCA ATCACTCTTC GAAAGTCTTC CGACTCCTCG 
GAACACATAT CAACGAAAAC TGCGAAAAGC AGCGAAGAGT CATTGGCGCC ATCCGCTTCC 
GGGACAGCGC TCTCTGCAGC TGAATCAATG GATGATCATT TATGGCCAGA GAGAGCAAGT 
CCATTTGCAT CGAGGCATGG CTCATTGAAG AGAAGAACGA GGGAACTTGA TCCCGAGAAA 
AAGGGGGACA AAACAAATAT TGTTCAAGAT CTACGTGCAA GATTTGAAAT GAAATCTCCA 
CCAAAATCAC CACCGAAAGA TGATGAAGAA GGAACATCTA CGGATCCCAA ACATTTCTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_504783.2
CDS	129..5291
Translation	MSNDNLAIEIDPPDDVEYDVEAGFNSSTCHLMHNFEEERPTSLVASIKESAISSKRKLKYYCSFICTPIKKLLSTFKLLIIIGLYSFIGAHIFMYLEVPTDLEAREDGFHQRKIAREVMVLNLRAIFYDNREDREERWKHAILKFEEDIGLEEPVIETVWTFWMSFLYAGTIFTTIGYGNIACKTRAGQIATMVYAFVGIPIMLVMLTSLNNFLLKWIKLITNGVSDMTLYIGVRLGITVIRQDEVQKRLRYTKLAKTMKRWKLSKHGAPSSIAISNSEENRLNSTPEDDEEEEEIHQDPPVLSTLIATVAWIILSAAVFCLFEDWTFFTSFYFCFISLTTIGLGDVTPANPEYMIATFGVVIVGLSMLTVCIDVLQEKLAQMYMALLQKLLTEYMEAVKSGDPNAASAMMAGFQGRAKFLMPLISKNEGAKVMDKFKQDCSKKGIDPPPVLTNINPETGMPAFANAPKEDFRDYIEVADEKFADEQQRLSQTPLSKSTDFLQSPTPRPTVTVAVTQTARPQACVSLEVQAGTTMGKFHDYGSQTQTESSDEGIQTDYSIDFDDEKNKEPEKVMADIGTQYEIVLLTSIGIQPEVSSIYIGAGLSDHSDEESGCESEDDSDDSDVSSDEESELKSSAKYKETPLPFEVEDPGMMSLGLQSPPIEITPFTDDESAASDARSSPVPPTSSKRSKLTPKTIKVGKSLSMDSTDTVIHVKPVGVNDDEKRSEEKESKDQETNDDLKTESGQEKKRRKKKKKLRSKKTREHELRFLKNSGNQASPLLMDCSTQYEIFTVESGSQYDVKGRSRKCQTKLSSINHEQLQGIRETVQKLRRRESEMSKVSNVSESDWDSETGKEPEVAEISLDLSVVALGVDESGKVVDIPKEVPKAVQRHESFSPTSELTTASESLSAEKVHLDSEDEELESLEELELYTTDAGIQTETKVMLDKQDNATPKLLEIIKNCSTQTTFSTFHIPQKVYESASTSSDEEDHKNDGEDEKQKKSKQEVFFMTNTGVQTMHTSFHIDEKPKFVEENLQTQNTHDGIKEQSAVDEEMPLFLKNSDCQTVEVISFECATSTENVSFVGDVETSEGSSQTAMQVSETEVQTMQQEFVVATMQTDEPLLVSDESQTEQTKSSEIDSQTDPPSTKSFICQTELITSSEVEVQAVRAVEFSLVECQTDALNHEEVEVQTELDISVSQSQTDNVDVGHIEMQTDMTITNASESQTDVLSVGDTEMQTDEKEIFTIESQTEQPSTELQETQTDDVSFAGDVPTTDQITQSESAQVFDSETQYALSIEEKDVQATPISFETQTQHSIELEVKSCQTEGVRMKNKKSQVEDDSMLSVLKIDSESQIEVDVSSINLSPIRLDVSNMFTMTEPGASEEKEVQVDEDCFEPEETSRNYYAGPLWSDFDYTPVIGEDGEEVMDGETQTQDGSEPGDPEFDEQRGQEQEEGEDDKERAHDQTAIGRSSRRKKIRKSQCISSENLLETPSSVQQSSDSLASESAVQCSPESYDMKIQTSKFKKKKKVEKNIEAKVEMSTSTVQATTEMCEQSAGSIEDDWLQVYVQELDEERRMRMSDIGIQTGVLARVQHIYTRPISDDVGPSSSAPITLRKSSDSSEHISTKTAKSSEESLAPSASGTALSAAESMDDHLWPERASPFASRHGSLKRRTRELDPEKKGDKTNIVQDLRARFEMKSPPKSPPKDDEEGTSTDPKHFF

Target ORF information:

RefSeq Version	NM_072382.7
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TWiK family of potassium channels (twk-11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_072382.7

ORF Insert Sequence:

ATGTCCAATG ATAACTTGGC GATTGAAATC GACCCTCCGG ACGATGTGGA GTATGATGTG 
GAAGCAGGCT TCAACTCTTC CACATGCCAT TTAATGCACA ATTTCGAAGA GGAACGTCCA 
ACCTCTCTCG TAGCCTCCAT AAAGGAAAGT GCCATTAGCT CAAAGCGAAA ACTAAAATAC 
TACTGCTCGT TCATATGCAC CCCAATCAAG AAGTTGTTAT CCACTTTCAA ACTACTCATC 
ATAATCGGTC TTTACTCATT TATTGGCGCT CATATATTCA TGTACTTGGA AGTTCCAACT 
GATTTAGAAG CAAGAGAAGA TGGATTTCAT CAAAGAAAAA TAGCGAGGGA AGTGATGGTG 
CTGAATCTCC GTGCAATTTT CTACGACAAC CGCGAAGATC GCGAGGAACG ATGGAAGCAT 
GCCATTTTGA AGTTCGAAGA GGACATTGGC TTAGAAGAAC CTGTCATTGA AACTGTATGG 
ACTTTTTGGA TGTCTTTCCT CTATGCAGGA ACTATTTTCA CAACAATAGG ATATGGAAAC 
ATTGCATGTA AGACGCGTGC TGGCCAGATA GCTACAATGG TATATGCTTT TGTCGGAATT 
CCGATCATGC TGGTGATGCT CACCTCGCTC AACAATTTTT TGTTAAAATG GATAAAACTT 
ATTACGAATG GAGTTAGTGA TATGACGCTT TATATTGGTG TTCGCTTGGG AATAACAGTT 
ATTCGGCAGG ACGAAGTCCA GAAGCGCCTT CGCTACACAA AACTTGCCAA GACAATGAAA 
AGGTGGAAAC TTTCAAAACA CGGAGCTCCA AGTAGTATAG CGATTAGCAA TTCGGAAGAA 
AACCGGTTAA ATTCAACACC TGAAGACGAC GAAGAAGAGG AAGAAATTCA TCAGGATCCT 
CCAGTTCTTT CGACTCTAAT TGCAACTGTT GCCTGGATTA TTCTTTCGGC TGCTGTGTTT 
TGTCTTTTTG AGGACTGGAC ATTTTTCACC AGTTTTTATT TTTGTTTCAT CTCTCTGACA 
ACAATCGGTC TTGGAGATGT GACACCTGCT AATCCAGAAT ATATGATTGC AACATTTGGA 
GTTGTGATTG TCGGTTTATC AATGCTCACT GTCTGTATCG ACGTCTTACA AGAAAAACTT 
GCTCAAATGT ATATGGCTCT ACTTCAGAAG TTATTGACAG AGTACATGGA AGCTGTGAAA 
AGCGGAGACC CAAATGCTGC ATCAGCTATG ATGGCTGGTT TTCAAGGAAG GGCAAAGTTT 
TTGATGCCGT TGATTAGTAA AAACGAAGGA GCAAAAGTTA TGGATAAGTT CAAACAAGAT 
TGTTCAAAGA AGGGAATCGA TCCACCACCG GTTTTAACAA ATATCAACCC GGAGACCGGA 
ATGCCAGCTT TTGCAAATGC TCCGAAAGAA GATTTTAGAG ATTATATTGA AGTTGCAGAT 
GAAAAATTTG CTGATGAACA ACAGAGGCTC TCTCAAACCC CGTTATCAAA ATCCACCGAC 
TTTTTACAAT CACCCACACC ACGTCCAACT GTCACTGTAG CTGTTACTCA AACAGCTAGA 
CCACAGGCAT GCGTATCACT AGAAGTACAG GCTGGAACCA CAATGGGAAA GTTCCATGAT 
TATGGATCAC AGACACAGAC GGAAAGTTCT GACGAAGGAA TTCAGACGGA TTATAGCATT 
GATTTTGATG ATGAAAAAAA CAAGGAGCCA GAGAAAGTTA TGGCGGACAT TGGCACTCAA 
TATGAAATCG TTCTATTAAC AAGTATTGGA ATTCAACCAG AAGTCTCATC AATTTATATT 
GGAGCCGGCT TATCTGATCA TTCGGACGAA GAAAGTGGAT GTGAATCAGA AGACGATTCG 
GATGACTCGG ATGTATCATC CGATGAGGAA AGTGAACTTA AATCTTCTGC GAAATACAAA 
GAAACACCTC TTCCATTTGA AGTCGAAGAT CCAGGAATGA TGTCGCTCGG ATTACAGTCT 
CCACCAATTG AAATAACTCC CTTCACGGAT GATGAATCAG CTGCAAGCGA CGCAAGAAGT 
TCTCCTGTCC CACCAACATC TTCCAAGCGA TCCAAGTTGA CACCAAAGAC TATAAAAGTT 
GGCAAATCGT TATCAATGGA CTCGACTGAT ACAGTTATTC ATGTGAAACC AGTTGGCGTT 
AATGATGATG AGAAAAGAAG TGAAGAAAAA GAAAGTAAAG ATCAAGAAAC AAATGATGAC 
CTAAAAACTG AAAGTGGACA GGAGAAAAAG AGAAGAAAGA AAAAGAAGAA GCTTCGTTCA 
AAGAAGACAA GAGAGCATGA GCTGAGATTT TTGAAGAATA GCGGAAATCA AGCATCTCCC 
CTTCTTATGG ATTGCTCTAC ACAATATGAG ATATTTACAG TGGAAAGTGG GTCGCAATAC 
GATGTGAAAG GAAGAAGCCG AAAATGTCAG ACAAAACTTT CCAGTATAAA TCATGAGCAG 
TTACAAGGGA TTCGAGAAAC AGTTCAAAAG TTGAGAAGAA GAGAAAGTGA AATGAGTAAG 
GTGTCGAATG TTTCTGAAAG TGACTGGGAT AGTGAAACTG GAAAAGAACC GGAAGTCGCT 
GAAATCAGTT TAGATCTTTC TGTAGTTGCT CTCGGAGTTG ATGAATCCGG GAAAGTTGTT 
GATATTCCTA AGGAAGTACC TAAAGCTGTT CAACGACATG AATCATTTTC ACCAACATCG 
GAGCTCACCA CTGCGTCCGA ATCACTTTCC GCTGAGAAAG TACATCTGGA TTCAGAAGAT 
GAGGAGTTAG AAAGTCTTGA AGAACTCGAA CTGTATACCA CAGACGCCGG AATTCAGACT 
GAAACAAAGG TCATGCTGGA CAAGCAAGAT AATGCAACTC CAAAGTTGCT GGAAATTATT 
AAAAACTGCA GCACTCAGAC AACATTCAGC ACATTCCACA TTCCGCAGAA GGTATATGAG 
TCGGCGAGCA CCTCAAGTGA CGAGGAAGAT CATAAAAACG ATGGTGAAGA TGAAAAACAG 
AAGAAATCTA AACAGGAAGT GTTCTTTATG ACCAACACTG GAGTACAGAC GATGCATACA 
TCGTTCCATA TTGATGAAAA ACCGAAATTT GTTGAAGAAA ATCTTCAAAC TCAAAATACC 
CACGACGGCA TAAAAGAACA AAGTGCCGTT GATGAAGAAA TGCCTCTGTT CTTAAAAAAT 
TCGGATTGTC AAACCGTTGA AGTAATATCA TTCGAATGTG CCACATCAAC AGAGAATGTT 
AGTTTTGTTG GAGACGTTGA AACTTCTGAA GGATCCAGTC AGACAGCCAT GCAAGTTTCT 
GAAACTGAAG TACAAACAAT GCAACAAGAA TTTGTGGTAG CAACAATGCA AACCGACGAG 
CCACTGTTGG TATCTGATGA GTCACAGACT GAGCAAACAA AATCTTCGGA AATTGATTCT 
CAAACTGATC CACCATCCAC AAAATCATTT ATATGTCAGA CAGAATTGAT AACAAGCTCG 
GAAGTTGAAG TTCAAGCAGT TCGAGCTGTA GAGTTTTCAC TTGTCGAATG CCAAACAGAT 
GCGCTAAATC ATGAAGAAGT AGAAGTTCAA ACAGAACTGG ATATTTCGGT TTCGCAGTCC 
CAAACTGATA ATGTTGATGT AGGCCACATT GAAATGCAAA CTGATATGAC AATAACAAAT 
GCATCGGAAT CTCAAACCGA TGTGCTCAGT GTAGGGGACA CGGAGATGCA AACAGACGAG 
AAAGAAATAT TCACAATAGA ATCGCAAACC GAACAACCAT CCACAGAACT GCAAGAGACT 
CAAACTGACG ATGTTTCTTT TGCTGGCGAT GTTCCAACAA CCGACCAAAT CACACAATCG 
GAATCTGCTC AAGTTTTCGA TTCTGAAACT CAATATGCTT TATCCATAGA AGAAAAGGAC 
GTCCAGGCAA CGCCAATCTC ATTTGAAACT CAGACACAAC ATTCCATTGA ACTTGAAGTG 
AAATCTTGCC AAACCGAAGG AGTTCGAATG AAAAACAAAA AGAGTCAGGT AGAAGACGAC 
TCAATGCTGT CAGTTCTCAA AATAGATAGT GAAAGTCAAA TAGAAGTGGA CGTTTCGTCC 
ATTAATCTTT CTCCAATTAG ATTAGATGTC TCCAATATGT TCACAATGAC AGAGCCAGGA 
GCTTCTGAAG AGAAAGAAGT ACAAGTTGAT GAGGACTGTT TTGAACCGGA GGAAACTTCA 
CGTAATTATT ATGCAGGACC GCTTTGGAGT GATTTTGATT ATACGCCGGT GATAGGCGAA 
GATGGGGAAG AAGTTATGGA CGGAGAGACT CAGACACAGG ATGGAAGCGA GCCAGGTGAT 
CCAGAATTTG ATGAACAGAG AGGACAGGAA CAAGAAGAAG GTGAAGATGA TAAAGAAAGA 
GCTCATGATC AAACTGCTAT CGGTAGATCA TCTCGTAGAA AGAAGATTAG AAAAAGTCAA 
TGCATCTCAT CAGAAAACTT GCTGGAAACA CCTTCAAGTG TCCAACAATC ATCAGATAGT 
CTTGCTTCTG AATCAGCAGT TCAATGTTCC CCCGAATCAT ATGATATGAA AATCCAAACG 
TCCAAGTTTA AAAAGAAAAA GAAGGTTGAG AAGAACATAG AAGCCAAGGT TGAAATGAGC 
ACTTCAACTG TGCAAGCAAC CACTGAAATG TGCGAGCAAA GTGCTGGAAG CATTGAAGAC 
GACTGGCTTC AGGTGTATGT TCAAGAACTC GATGAAGAAC GTCGAATGCG TATGTCAGAC 
ATTGGCATAC AAACCGGTGT TCTGGCACGT GTTCAACACA TTTATACCCG TCCAATATCT 
GATGATGTGG GACCATCTTC ATCTGCTCCA ATCACTCTTC GAAAGTCTTC CGACTCCTCG 
GAACACATAT CAACGAAAAC TGCGAAAAGC AGCGAAGAGT CATTGGCGCC ATCCGCTTCC 
GGGACAGCGC TCTCTGCAGC TGAATCAATG GATGATCATT TATGGCCAGA GAGAGCAAGT 
CCATTTGCAT CGAGGCATGG CTCATTGAAG AGAAGAACGA GGGAACTTGA TCCCGAGAAA 
AAGGGGGACA AAACAAATAT TGTTCAAGAT CTACGTGCAA GATTTGAAAT GAAATCTCCA 
CCAAAATCAC CACCGAAAGA TGATGAAGAA GGAACATCTA CGGATCCCAA ACATTTCTTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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twk-11 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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