cft-1 cDNA ORF clone, Caenorhabditis elegans

The following cft-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cft-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OCa347068 NM_072062.5
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Caenorhabditis elegans Cystic Fibrosis Transmembrane conductance regulator homolog (cft-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
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OCa347068 NM_072062.4 Caenorhabditis elegans Cystic Fibrosis Transmembrane conductance regulator homolog (cft-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa347068
    Clone ID Related Accession (Same CDS sequence) NM_072062.4 , NM_072062.5
    Accession Version NM_072062.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Cystic Fibrosis Transmembrane conductance regulator homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_072062.3. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTATTATT CAGTGAGTGT GAATCTATCG AAGAACGCGC GGCAAATTCA AAATGAAACT 
    GAAAATGAGC AAGCGAAGAG CTCATTTGCA TCGAAACTTT TAGAGCTTCC GGTCTCAAAG
    TTATTACAGA AATGTAGATC ATCTACGATT ACTGTAGATG ATCTACCCAT TCTTGAAGAA
    AAACAATGTT CAAACCGAGT TATGAGTGGA TTTAAATGGA AGAAACGAGT AATTTTCTCA
    ATTCTAATGA AATTCTGGAA GTCTTTCGCT GGAGCATTCG CCTTCAGAAT GTGTGCCATT
    TTTGCCGATT ACGCCTCCAT TTTGCTTCTC AAATATCTGA TTGATTCTGC AGAAATCTCT
    GCAACTTTAT TCAATTTTTT CATTATCGCT GTGCTGATTC TATTCTTCAT GCAGCTGAAA
    TCTTTACTCC TTGGTGTTCA CACGTATCTT GTTGGAGAAG ACAGCGTGGA AATGGTTACC
    GTTTTGAACA ATATTATAGT GAAAAAGGCT CTTCATCTAA CATCCGAGAA CACCGACTGG
    AACAGATCGA AGATTTCAAA ACTTCTGACA ACACATTCAG AAGCCGTCTC AAACGGTCTT
    GCATATGTTC ATCACTCGTG TTCGGCTCTA ATCGAGCTCA CAATTGCATT GTTTTGGATC
    TGGGAAACAC TTGGAACTCG TACAATCATC GTCGTTTTGA TTGTTGCACT GGTTTATGCC
    GTTTTCAATA TAATTGACTC GTATGTCTAC AAAAAGAGCT TGAAAGTGCA ACTCAAATGC
    CGTGATGAAC GTGTGGAATT TGAGAAAACT GTGTTAAATT CAATGGAAAC CATCAAAATG
    TTCTCGTGGG AACCTCATTT TTGGAAGAAG TTAAAGTCTA TGAGAGGGAA AGAGCTATCT
    GTGTTCCGAA AAACTCTTTC TATCAACAGC TTCATGCATT CTCTGAATGT CACATCTCCT
    TTCGTTATTA CTTTTGTGAG TTTCGCAGTT TACGGACTTC AATATGAAAT CAGTGCTCTT
    CGTTTTGAAG ATGCATTTGT TTTAATTGCA ATTTTCAACT ATATGAGAAG ACCGTTGCAT
    GTCATTATTC CATCTCTGGA AATTGTTGAT AAAGCTCTAC ATTCTGCAAC CATCATCAAC
    CAATTCCTCA TTGTGAAACA ATCTCCAAAA ATTAACACCA AAACTGCACC CCAACCTCCT
    GTTGAACCAG ATTTGGATAT CAAAATCTTG AACGCCAATT TCTCATGGAA CGGAAAAGAT
    GATACATTGA ATGATGTATG TGTTCATGTG AAGAAAGGAG AAAAACATGC GTTCATCGGA
    TTCCCCTTAT GCGGAAAATC GTCACTGTTG TTTTCAATCA CTGGAGAGTT GAAAATGACA
    AAAGGAAATA TGTGGGTCTC GAAGGGAATT TCATTTGCTC CTGCAAGTCC ATACATATTC
    TCTCAGTCAC TGAAGGAAAA CATTTTATTT GGAAATGACT ATGTGAAATC CAAATATGAT
    AAGGTAATCA TGGCTTGTGA CCTGAAGAAA GATATATTTT CTCTACCGAG ATGTGAAGCT
    ACAGTTCTAG GAGATAAGGG ATATGTGTTA ACTTCAACTC AAAAAGCTCA AATATCTCTG
    GCGAGATGTC TCTACGAGGA TGCTGATATT TATGCTTTAG ACAAAGCATT TGGGCCAATG
    GATCGGACGA CTTCAAATAA AATTTTTACA AGAGTTCTTG GTGAGAATGG TTATCTACGT
    GATAAAACAG TTATCCTGGC TACAAATAAC ATTGAACTGA TCAAAACTTT CACGATGGTT
    CATGTATTGA AAAATGGGAA AATTGAAACA AGCGGAAGTT ATGATCAAAT CCTGGAGTGT
    AGTGAAATTA TGAGTGATCT TTTGAATCAG AGCCGGATTG ATAAAATCAT TGAGGAAAAG
    TATTCAGATG AAAAACCACC GAGAAAAACT GTAATGTTTG ATGACAAAGT ATTGAAAAAA
    CGAAAAAAGA AGCAATCAAT GGAAGATACA GCTCCAGTAT TTGAGAATAG AAGTGTTTAT
    CCATTCTACT TCCGTAGTGG ATCCTTCATA TTCACAGCTT TTTACATCGT TTTACTGATT
    TGTCGATTCG TCTTCCAGGC GCTTGCCTTC TTTTGGCTCT CTTTCTGGTT GGACCCAGTG
    TGGAAAAAAG TGGAATGTGA AAATTGTCCA CAATACGTGT TCATTCAAAC AATGTCATTT
    TTTGCGATTT CAGCTATAAT ATCCACAATG TTGTCATACA CATTCTTCGT TCTAACAAAT
    GTTCAGACTT CGAGAAAGCT TTATCAACAT ATTACAAGTG CGATTTTTGC GGTGCCAATG
    ATATTTTTAT CGCCGACCAA CAAAGAGTCG TTGATGAATA TGATCACATC TGATCTTGAC
    ATTGTGGACA CCCAGTTTCC TCTATTTTTC AAATTCTCAT TTGAATGCAC CCTCCACATT
    CTTATGATAT TCGCTATAGT CTGTGTGAAT GTGCCAGTTT TTGCGATTTT CGTGGTGGCA
    TTCTTGATAT TCCTTGTCGG ACTACTTCGG TATTTCCTGC CAGCTCTTCA CAAAATTTCG
    AGCCTCGAGG AACAAAAACG AGACTTATTT CTGTGCGGGT CTATTGAAGA TTTTGAAGCA
    AGAATTATGG TGCGAACGTT TGGAAAGACA CGCCAAACGC TTGCAAAAAC TAGTGTGAAT
    GCAGATATAC TGACGAGATG CCGAATGGCA AGATATTCAA CTCTCAGATG GATTTCTCTT
    CGAACTGAGT TCATCTCAAA TCTCATGATT TGTATGTGTT TCTTCATCGC ATCAATTTGT
    TTGAAAGTGG ATTACATTGG AAATGCACAA TTCGCTCTTT CAGTTGCATC AATTCTATGT
    ATTAGCGAAC TTGTTTCAAC ATTCATCCGA ACCACGTGCA TCCTTGAATC TCTAACTCCA
    CACGTTCAAC GTATCAGCAA TGTGAGAGAA TATCCAAAAG AGCCCATGAT TGATAAATGC
    ACCATCCGAG ACTCTTGGCC GGACGACGGA AAAATTGAAA TCAAAAACTT GAATGTATTC
    GCCAACAAGT ACAGACACGT CATTAAAGAT GTCTCCTTGG TAATAGAAGA ACGAGAGAAA
    CTGGGAATTA TCGGTAAAGT TGGATCGGGA AAATCTCAAC TGGCAAAGAC ATTGGTCATG
    ATGAGCTCAG CAGACAAAGA GAGCCATATC GTTGTAGATG ATTTGGACAT TTTCGAGATG
    AGTGTAAAAA CTTTGAGATC AAGGATCACA GTTATACCAC AGAAAGCAAA AATATTCTCG
    GACACTCTCC GTAGCAACAT TGACCCATGT TGCCAGTTTG CTGATAGTGA TATTTGGTTG
    GCAATTGAGG CATGTCAACT CAGAGAATAT GTGAAAAGTC TACCGGATGG TTTGCATCAA
    ATGGTGTCTC CTGAAAAAAT GAGTAATGAG CAGAAATGTC AGGTGAATGT TTGCCGAGCA
    CTTCTAAAAG GTGGTCAGAT CTTTGTGATT GATCAATCGA CGAAGATGAT GAAAGAACCA
    ATCAAGTCTT TGGTGAATGA GGCAATAAGA AATAGTTTGA AACAAAGTAC TACAATTTGG
    ATTGGTGAAG ACTTTTCCGA TGTTGAACAT TGTGATAGAG TCGTTGTAAT TGAGAATGGT
    GTAATTCTCA CAACGGACAC TCCACAAAAC TTGATTTCGG AATTCGGATC CCTTCAAAAA
    TGCCTTTTGA ACTTTTCGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_504463.1
    CDS1..3744
    Translation

    Target ORF information:

    RefSeq Version NM_072062.4
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Cystic Fibrosis Transmembrane conductance regulator homolog (cft-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_072062.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTATTATT CAGTGAGTGT GAATCTATCG AAGAACGCGC GGCAAATTCA AAATGAAACT 
    GAAAATGAGC AAGCGAAGAG CTCATTTGCA TCGAAACTTT TAGAGCTTCC GGTCTCAAAG
    TTATTACAGA AATGTAGATC ATCTACGATT ACTGTAGATG ATCTACCCAT TCTTGAAGAA
    AAACAATGTT CAAACCGAGT TATGAGTGGA TTTAAATGGA AGAAACGAGT AATTTTCTCA
    ATTCTAATGA AATTCTGGAA GTCTTTCGCT GGAGCATTCG CCTTCAGAAT GTGTGCCATT
    TTTGCCGATT ACGCCTCCAT TTTGCTTCTC AAATATCTGA TTGATTCTGC AGAAATCTCT
    GCAACTTTAT TCAATTTTTT CATTATCGCT GTGCTGATTC TATTCTTCAT GCAGCTGAAA
    TCTTTACTCC TTGGTGTTCA CACGTATCTT GTTGGAGAAG ACAGCGTGGA AATGGTTACC
    GTTTTGAACA ATATTATAGT GAAAAAGGCT CTTCATCTAA CATCCGAGAA CACCGACTGG
    AACAGATCGA AGATTTCAAA ACTTCTGACA ACACATTCAG AAGCCGTCTC AAACGGTCTT
    GCATATGTTC ATCACTCGTG TTCGGCTCTA ATCGAGCTCA CAATTGCATT GTTTTGGATC
    TGGGAAACAC TTGGAACTCG TACAATCATC GTCGTTTTGA TTGTTGCACT GGTTTATGCC
    GTTTTCAATA TAATTGACTC GTATGTCTAC AAAAAGAGCT TGAAAGTGCA ACTCAAATGC
    CGTGATGAAC GTGTGGAATT TGAGAAAACT GTGTTAAATT CAATGGAAAC CATCAAAATG
    TTCTCGTGGG AACCTCATTT TTGGAAGAAG TTAAAGTCTA TGAGAGGGAA AGAGCTATCT
    GTGTTCCGAA AAACTCTTTC TATCAACAGC TTCATGCATT CTCTGAATGT CACATCTCCT
    TTCGTTATTA CTTTTGTGAG TTTCGCAGTT TACGGACTTC AATATGAAAT CAGTGCTCTT
    CGTTTTGAAG ATGCATTTGT TTTAATTGCA ATTTTCAACT ATATGAGAAG ACCGTTGCAT
    GTCATTATTC CATCTCTGGA AATTGTTGAT AAAGCTCTAC ATTCTGCAAC CATCATCAAC
    CAATTCCTCA TTGTGAAACA ATCTCCAAAA ATTAACACCA AAACTGCACC CCAACCTCCT
    GTTGAACCAG ATTTGGATAT CAAAATCTTG AACGCCAATT TCTCATGGAA CGGAAAAGAT
    GATACATTGA ATGATGTATG TGTTCATGTG AAGAAAGGAG AAAAACATGC GTTCATCGGA
    TTCCCCTTAT GCGGAAAATC GTCACTGTTG TTTTCAATCA CTGGAGAGTT GAAAATGACA
    AAAGGAAATA TGTGGGTCTC GAAGGGAATT TCATTTGCTC CTGCAAGTCC ATACATATTC
    TCTCAGTCAC TGAAGGAAAA CATTTTATTT GGAAATGACT ATGTGAAATC CAAATATGAT
    AAGGTAATCA TGGCTTGTGA CCTGAAGAAA GATATATTTT CTCTACCGAG ATGTGAAGCT
    ACAGTTCTAG GAGATAAGGG ATATGTGTTA ACTTCAACTC AAAAAGCTCA AATATCTCTG
    GCGAGATGTC TCTACGAGGA TGCTGATATT TATGCTTTAG ACAAAGCATT TGGGCCAATG
    GATCGGACGA CTTCAAATAA AATTTTTACA AGAGTTCTTG GTGAGAATGG TTATCTACGT
    GATAAAACAG TTATCCTGGC TACAAATAAC ATTGAACTGA TCAAAACTTT CACGATGGTT
    CATGTATTGA AAAATGGGAA AATTGAAACA AGCGGAAGTT ATGATCAAAT CCTGGAGTGT
    AGTGAAATTA TGAGTGATCT TTTGAATCAG AGCCGGATTG ATAAAATCAT TGAGGAAAAG
    TATTCAGATG AAAAACCACC GAGAAAAACT GTAATGTTTG ATGACAAAGT ATTGAAAAAA
    CGAAAAAAGA AGCAATCAAT GGAAGATACA GCTCCAGTAT TTGAGAATAG AAGTGTTTAT
    CCATTCTACT TCCGTAGTGG ATCCTTCATA TTCACAGCTT TTTACATCGT TTTACTGATT
    TGTCGATTCG TCTTCCAGGC GCTTGCCTTC TTTTGGCTCT CTTTCTGGTT GGACCCAGTG
    TGGAAAAAAG TGGAATGTGA AAATTGTCCA CAATACGTGT TCATTCAAAC AATGTCATTT
    TTTGCGATTT CAGCTATAAT ATCCACAATG TTGTCATACA CATTCTTCGT TCTAACAAAT
    GTTCAGACTT CGAGAAAGCT TTATCAACAT ATTACAAGTG CGATTTTTGC GGTGCCAATG
    ATATTTTTAT CGCCGACCAA CAAAGAGTCG TTGATGAATA TGATCACATC TGATCTTGAC
    ATTGTGGACA CCCAGTTTCC TCTATTTTTC AAATTCTCAT TTGAATGCAC CCTCCACATT
    CTTATGATAT TCGCTATAGT CTGTGTGAAT GTGCCAGTTT TTGCGATTTT CGTGGTGGCA
    TTCTTGATAT TCCTTGTCGG ACTACTTCGG TATTTCCTGC CAGCTCTTCA CAAAATTTCG
    AGCCTCGAGG AACAAAAACG AGACTTATTT CTGTGCGGGT CTATTGAAGA TTTTGAAGCA
    AGAATTATGG TGCGAACGTT TGGAAAGACA CGCCAAACGC TTGCAAAAAC TAGTGTGAAT
    GCAGATATAC TGACGAGATG CCGAATGGCA AGATATTCAA CTCTCAGATG GATTTCTCTT
    CGAACTGAGT TCATCTCAAA TCTCATGATT TGTATGTGTT TCTTCATCGC ATCAATTTGT
    TTGAAAGTGG ATTACATTGG AAATGCACAA TTCGCTCTTT CAGTTGCATC AATTCTATGT
    ATTAGCGAAC TTGTTTCAAC ATTCATCCGA ACCACGTGCA TCCTTGAATC TCTAACTCCA
    CACGTTCAAC GTATCAGCAA TGTGAGAGAA TATCCAAAAG AGCCCATGAT TGATAAATGC
    ACCATCCGAG ACTCTTGGCC GGACGACGGA AAAATTGAAA TCAAAAACTT GAATGTATTC
    GCCAACAAGT ACAGACACGT CATTAAAGAT GTCTCCTTGG TAATAGAAGA ACGAGAGAAA
    CTGGGAATTA TCGGTAAAGT TGGATCGGGA AAATCTCAAC TGGCAAAGAC ATTGGTCATG
    ATGAGCTCAG CAGACAAAGA GAGCCATATC GTTGTAGATG ATTTGGACAT TTTCGAGATG
    AGTGTAAAAA CTTTGAGATC AAGGATCACA GTTATACCAC AGAAAGCAAA AATATTCTCG
    GACACTCTCC GTAGCAACAT TGACCCATGT TGCCAGTTTG CTGATAGTGA TATTTGGTTG
    GCAATTGAGG CATGTCAACT CAGAGAATAT GTGAAAAGTC TACCGGATGG TTTGCATCAA
    ATGGTGTCTC CTGAAAAAAT GAGTAATGAG CAGAAATGTC AGGTGAATGT TTGCCGAGCA
    CTTCTAAAAG GTGGTCAGAT CTTTGTGATT GATCAATCGA CGAAGATGAT GAAAGAACCA
    ATCAAGTCTT TGGTGAATGA GGCAATAAGA AATAGTTTGA AACAAAGTAC TACAATTTGG
    ATTGGTGAAG ACTTTTCCGA TGTTGAACAT TGTGATAGAG TCGTTGTAAT TGAGAATGGT
    GTAATTCTCA CAACGGACAC TCCACAAAAC TTGATTTCGG AATTCGGATC CCTTCAAAAA
    TGCCTTTTGA ACTTTTCGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa347068
    Clone ID Related Accession (Same CDS sequence) NM_072062.4 , NM_072062.5
    Accession Version NM_072062.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Cystic Fibrosis Transmembrane conductance regulator homolog
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_072062.4. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTATTATT CAGTGAGTGT GAATCTATCG AAGAACGCGC GGCAAATTCA AAATGAAACT 
    GAAAATGAGC AAGCGAAGAG CTCATTTGCA TCGAAACTTT TAGAGCTTCC GGTCTCAAAG
    TTATTACAGA AATGTAGATC ATCTACGATT ACTGTAGATG ATCTACCCAT TCTTGAAGAA
    AAACAATGTT CAAACCGAGT TATGAGTGGA TTTAAATGGA AGAAACGAGT AATTTTCTCA
    ATTCTAATGA AATTCTGGAA GTCTTTCGCT GGAGCATTCG CCTTCAGAAT GTGTGCCATT
    TTTGCCGATT ACGCCTCCAT TTTGCTTCTC AAATATCTGA TTGATTCTGC AGAAATCTCT
    GCAACTTTAT TCAATTTTTT CATTATCGCT GTGCTGATTC TATTCTTCAT GCAGCTGAAA
    TCTTTACTCC TTGGTGTTCA CACGTATCTT GTTGGAGAAG ACAGCGTGGA AATGGTTACC
    GTTTTGAACA ATATTATAGT GAAAAAGGCT CTTCATCTAA CATCCGAGAA CACCGACTGG
    AACAGATCGA AGATTTCAAA ACTTCTGACA ACACATTCAG AAGCCGTCTC AAACGGTCTT
    GCATATGTTC ATCACTCGTG TTCGGCTCTA ATCGAGCTCA CAATTGCATT GTTTTGGATC
    TGGGAAACAC TTGGAACTCG TACAATCATC GTCGTTTTGA TTGTTGCACT GGTTTATGCC
    GTTTTCAATA TAATTGACTC GTATGTCTAC AAAAAGAGCT TGAAAGTGCA ACTCAAATGC
    CGTGATGAAC GTGTGGAATT TGAGAAAACT GTGTTAAATT CAATGGAAAC CATCAAAATG
    TTCTCGTGGG AACCTCATTT TTGGAAGAAG TTAAAGTCTA TGAGAGGGAA AGAGCTATCT
    GTGTTCCGAA AAACTCTTTC TATCAACAGC TTCATGCATT CTCTGAATGT CACATCTCCT
    TTCGTTATTA CTTTTGTGAG TTTCGCAGTT TACGGACTTC AATATGAAAT CAGTGCTCTT
    CGTTTTGAAG ATGCATTTGT TTTAATTGCA ATTTTCAACT ATATGAGAAG ACCGTTGCAT
    GTCATTATTC CATCTCTGGA AATTGTTGAT AAAGCTCTAC ATTCTGCAAC CATCATCAAC
    CAATTCCTCA TTGTGAAACA ATCTCCAAAA ATTAACACCA AAACTGCACC CCAACCTCCT
    GTTGAACCAG ATTTGGATAT CAAAATCTTG AACGCCAATT TCTCATGGAA CGGAAAAGAT
    GATACATTGA ATGATGTATG TGTTCATGTG AAGAAAGGAG AAAAACATGC GTTCATCGGA
    TTCCCCTTAT GCGGAAAATC GTCACTGTTG TTTTCAATCA CTGGAGAGTT GAAAATGACA
    AAAGGAAATA TGTGGGTCTC GAAGGGAATT TCATTTGCTC CTGCAAGTCC ATACATATTC
    TCTCAGTCAC TGAAGGAAAA CATTTTATTT GGAAATGACT ATGTGAAATC CAAATATGAT
    AAGGTAATCA TGGCTTGTGA CCTGAAGAAA GATATATTTT CTCTACCGAG ATGTGAAGCT
    ACAGTTCTAG GAGATAAGGG ATATGTGTTA ACTTCAACTC AAAAAGCTCA AATATCTCTG
    GCGAGATGTC TCTACGAGGA TGCTGATATT TATGCTTTAG ACAAAGCATT TGGGCCAATG
    GATCGGACGA CTTCAAATAA AATTTTTACA AGAGTTCTTG GTGAGAATGG TTATCTACGT
    GATAAAACAG TTATCCTGGC TACAAATAAC ATTGAACTGA TCAAAACTTT CACGATGGTT
    CATGTATTGA AAAATGGGAA AATTGAAACA AGCGGAAGTT ATGATCAAAT CCTGGAGTGT
    AGTGAAATTA TGAGTGATCT TTTGAATCAG AGCCGGATTG ATAAAATCAT TGAGGAAAAG
    TATTCAGATG AAAAACCACC GAGAAAAACT GTAATGTTTG ATGACAAAGT ATTGAAAAAA
    CGAAAAAAGA AGCAATCAAT GGAAGATACA GCTCCAGTAT TTGAGAATAG AAGTGTTTAT
    CCATTCTACT TCCGTAGTGG ATCCTTCATA TTCACAGCTT TTTACATCGT TTTACTGATT
    TGTCGATTCG TCTTCCAGGC GCTTGCCTTC TTTTGGCTCT CTTTCTGGTT GGACCCAGTG
    TGGAAAAAAG TGGAATGTGA AAATTGTCCA CAATACGTGT TCATTCAAAC AATGTCATTT
    TTTGCGATTT CAGCTATAAT ATCCACAATG TTGTCATACA CATTCTTCGT TCTAACAAAT
    GTTCAGACTT CGAGAAAGCT TTATCAACAT ATTACAAGTG CGATTTTTGC GGTGCCAATG
    ATATTTTTAT CGCCGACCAA CAAAGAGTCG TTGATGAATA TGATCACATC TGATCTTGAC
    ATTGTGGACA CCCAGTTTCC TCTATTTTTC AAATTCTCAT TTGAATGCAC CCTCCACATT
    CTTATGATAT TCGCTATAGT CTGTGTGAAT GTGCCAGTTT TTGCGATTTT CGTGGTGGCA
    TTCTTGATAT TCCTTGTCGG ACTACTTCGG TATTTCCTGC CAGCTCTTCA CAAAATTTCG
    AGCCTCGAGG AACAAAAACG AGACTTATTT CTGTGCGGGT CTATTGAAGA TTTTGAAGCA
    AGAATTATGG TGCGAACGTT TGGAAAGACA CGCCAAACGC TTGCAAAAAC TAGTGTGAAT
    GCAGATATAC TGACGAGATG CCGAATGGCA AGATATTCAA CTCTCAGATG GATTTCTCTT
    CGAACTGAGT TCATCTCAAA TCTCATGATT TGTATGTGTT TCTTCATCGC ATCAATTTGT
    TTGAAAGTGG ATTACATTGG AAATGCACAA TTCGCTCTTT CAGTTGCATC AATTCTATGT
    ATTAGCGAAC TTGTTTCAAC ATTCATCCGA ACCACGTGCA TCCTTGAATC TCTAACTCCA
    CACGTTCAAC GTATCAGCAA TGTGAGAGAA TATCCAAAAG AGCCCATGAT TGATAAATGC
    ACCATCCGAG ACTCTTGGCC GGACGACGGA AAAATTGAAA TCAAAAACTT GAATGTATTC
    GCCAACAAGT ACAGACACGT CATTAAAGAT GTCTCCTTGG TAATAGAAGA ACGAGAGAAA
    CTGGGAATTA TCGGTAAAGT TGGATCGGGA AAATCTCAAC TGGCAAAGAC ATTGGTCATG
    ATGAGCTCAG CAGACAAAGA GAGCCATATC GTTGTAGATG ATTTGGACAT TTTCGAGATG
    AGTGTAAAAA CTTTGAGATC AAGGATCACA GTTATACCAC AGAAAGCAAA AATATTCTCG
    GACACTCTCC GTAGCAACAT TGACCCATGT TGCCAGTTTG CTGATAGTGA TATTTGGTTG
    GCAATTGAGG CATGTCAACT CAGAGAATAT GTGAAAAGTC TACCGGATGG TTTGCATCAA
    ATGGTGTCTC CTGAAAAAAT GAGTAATGAG CAGAAATGTC AGGTGAATGT TTGCCGAGCA
    CTTCTAAAAG GTGGTCAGAT CTTTGTGATT GATCAATCGA CGAAGATGAT GAAAGAACCA
    ATCAAGTCTT TGGTGAATGA GGCAATAAGA AATAGTTTGA AACAAAGTAC TACAATTTGG
    ATTGGTGAAG ACTTTTCCGA TGTTGAACAT TGTGATAGAG TCGTTGTAAT TGAGAATGGT
    GTAATTCTCA CAACGGACAC TCCACAAAAC TTGATTTCGG AATTCGGATC CCTTCAAAAA
    TGCCTTTTGA ACTTTTCGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_504463.1
    CDS1..3744
    Translation

    Target ORF information:

    RefSeq Version NM_072062.5
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Cystic Fibrosis Transmembrane conductance regulator homolog (cft-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_072062.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTATTATT CAGTGAGTGT GAATCTATCG AAGAACGCGC GGCAAATTCA AAATGAAACT 
    GAAAATGAGC AAGCGAAGAG CTCATTTGCA TCGAAACTTT TAGAGCTTCC GGTCTCAAAG
    TTATTACAGA AATGTAGATC ATCTACGATT ACTGTAGATG ATCTACCCAT TCTTGAAGAA
    AAACAATGTT CAAACCGAGT TATGAGTGGA TTTAAATGGA AGAAACGAGT AATTTTCTCA
    ATTCTAATGA AATTCTGGAA GTCTTTCGCT GGAGCATTCG CCTTCAGAAT GTGTGCCATT
    TTTGCCGATT ACGCCTCCAT TTTGCTTCTC AAATATCTGA TTGATTCTGC AGAAATCTCT
    GCAACTTTAT TCAATTTTTT CATTATCGCT GTGCTGATTC TATTCTTCAT GCAGCTGAAA
    TCTTTACTCC TTGGTGTTCA CACGTATCTT GTTGGAGAAG ACAGCGTGGA AATGGTTACC
    GTTTTGAACA ATATTATAGT GAAAAAGGCT CTTCATCTAA CATCCGAGAA CACCGACTGG
    AACAGATCGA AGATTTCAAA ACTTCTGACA ACACATTCAG AAGCCGTCTC AAACGGTCTT
    GCATATGTTC ATCACTCGTG TTCGGCTCTA ATCGAGCTCA CAATTGCATT GTTTTGGATC
    TGGGAAACAC TTGGAACTCG TACAATCATC GTCGTTTTGA TTGTTGCACT GGTTTATGCC
    GTTTTCAATA TAATTGACTC GTATGTCTAC AAAAAGAGCT TGAAAGTGCA ACTCAAATGC
    CGTGATGAAC GTGTGGAATT TGAGAAAACT GTGTTAAATT CAATGGAAAC CATCAAAATG
    TTCTCGTGGG AACCTCATTT TTGGAAGAAG TTAAAGTCTA TGAGAGGGAA AGAGCTATCT
    GTGTTCCGAA AAACTCTTTC TATCAACAGC TTCATGCATT CTCTGAATGT CACATCTCCT
    TTCGTTATTA CTTTTGTGAG TTTCGCAGTT TACGGACTTC AATATGAAAT CAGTGCTCTT
    CGTTTTGAAG ATGCATTTGT TTTAATTGCA ATTTTCAACT ATATGAGAAG ACCGTTGCAT
    GTCATTATTC CATCTCTGGA AATTGTTGAT AAAGCTCTAC ATTCTGCAAC CATCATCAAC
    CAATTCCTCA TTGTGAAACA ATCTCCAAAA ATTAACACCA AAACTGCACC CCAACCTCCT
    GTTGAACCAG ATTTGGATAT CAAAATCTTG AACGCCAATT TCTCATGGAA CGGAAAAGAT
    GATACATTGA ATGATGTATG TGTTCATGTG AAGAAAGGAG AAAAACATGC GTTCATCGGA
    TTCCCCTTAT GCGGAAAATC GTCACTGTTG TTTTCAATCA CTGGAGAGTT GAAAATGACA
    AAAGGAAATA TGTGGGTCTC GAAGGGAATT TCATTTGCTC CTGCAAGTCC ATACATATTC
    TCTCAGTCAC TGAAGGAAAA CATTTTATTT GGAAATGACT ATGTGAAATC CAAATATGAT
    AAGGTAATCA TGGCTTGTGA CCTGAAGAAA GATATATTTT CTCTACCGAG ATGTGAAGCT
    ACAGTTCTAG GAGATAAGGG ATATGTGTTA ACTTCAACTC AAAAAGCTCA AATATCTCTG
    GCGAGATGTC TCTACGAGGA TGCTGATATT TATGCTTTAG ACAAAGCATT TGGGCCAATG
    GATCGGACGA CTTCAAATAA AATTTTTACA AGAGTTCTTG GTGAGAATGG TTATCTACGT
    GATAAAACAG TTATCCTGGC TACAAATAAC ATTGAACTGA TCAAAACTTT CACGATGGTT
    CATGTATTGA AAAATGGGAA AATTGAAACA AGCGGAAGTT ATGATCAAAT CCTGGAGTGT
    AGTGAAATTA TGAGTGATCT TTTGAATCAG AGCCGGATTG ATAAAATCAT TGAGGAAAAG
    TATTCAGATG AAAAACCACC GAGAAAAACT GTAATGTTTG ATGACAAAGT ATTGAAAAAA
    CGAAAAAAGA AGCAATCAAT GGAAGATACA GCTCCAGTAT TTGAGAATAG AAGTGTTTAT
    CCATTCTACT TCCGTAGTGG ATCCTTCATA TTCACAGCTT TTTACATCGT TTTACTGATT
    TGTCGATTCG TCTTCCAGGC GCTTGCCTTC TTTTGGCTCT CTTTCTGGTT GGACCCAGTG
    TGGAAAAAAG TGGAATGTGA AAATTGTCCA CAATACGTGT TCATTCAAAC AATGTCATTT
    TTTGCGATTT CAGCTATAAT ATCCACAATG TTGTCATACA CATTCTTCGT TCTAACAAAT
    GTTCAGACTT CGAGAAAGCT TTATCAACAT ATTACAAGTG CGATTTTTGC GGTGCCAATG
    ATATTTTTAT CGCCGACCAA CAAAGAGTCG TTGATGAATA TGATCACATC TGATCTTGAC
    ATTGTGGACA CCCAGTTTCC TCTATTTTTC AAATTCTCAT TTGAATGCAC CCTCCACATT
    CTTATGATAT TCGCTATAGT CTGTGTGAAT GTGCCAGTTT TTGCGATTTT CGTGGTGGCA
    TTCTTGATAT TCCTTGTCGG ACTACTTCGG TATTTCCTGC CAGCTCTTCA CAAAATTTCG
    AGCCTCGAGG AACAAAAACG AGACTTATTT CTGTGCGGGT CTATTGAAGA TTTTGAAGCA
    AGAATTATGG TGCGAACGTT TGGAAAGACA CGCCAAACGC TTGCAAAAAC TAGTGTGAAT
    GCAGATATAC TGACGAGATG CCGAATGGCA AGATATTCAA CTCTCAGATG GATTTCTCTT
    CGAACTGAGT TCATCTCAAA TCTCATGATT TGTATGTGTT TCTTCATCGC ATCAATTTGT
    TTGAAAGTGG ATTACATTGG AAATGCACAA TTCGCTCTTT CAGTTGCATC AATTCTATGT
    ATTAGCGAAC TTGTTTCAAC ATTCATCCGA ACCACGTGCA TCCTTGAATC TCTAACTCCA
    CACGTTCAAC GTATCAGCAA TGTGAGAGAA TATCCAAAAG AGCCCATGAT TGATAAATGC
    ACCATCCGAG ACTCTTGGCC GGACGACGGA AAAATTGAAA TCAAAAACTT GAATGTATTC
    GCCAACAAGT ACAGACACGT CATTAAAGAT GTCTCCTTGG TAATAGAAGA ACGAGAGAAA
    CTGGGAATTA TCGGTAAAGT TGGATCGGGA AAATCTCAAC TGGCAAAGAC ATTGGTCATG
    ATGAGCTCAG CAGACAAAGA GAGCCATATC GTTGTAGATG ATTTGGACAT TTTCGAGATG
    AGTGTAAAAA CTTTGAGATC AAGGATCACA GTTATACCAC AGAAAGCAAA AATATTCTCG
    GACACTCTCC GTAGCAACAT TGACCCATGT TGCCAGTTTG CTGATAGTGA TATTTGGTTG
    GCAATTGAGG CATGTCAACT CAGAGAATAT GTGAAAAGTC TACCGGATGG TTTGCATCAA
    ATGGTGTCTC CTGAAAAAAT GAGTAATGAG CAGAAATGTC AGGTGAATGT TTGCCGAGCA
    CTTCTAAAAG GTGGTCAGAT CTTTGTGATT GATCAATCGA CGAAGATGAT GAAAGAACCA
    ATCAAGTCTT TGGTGAATGA GGCAATAAGA AATAGTTTGA AACAAAGTAC TACAATTTGG
    ATTGGTGAAG ACTTTTCCGA TGTTGAACAT TGTGATAGAG TCGTTGTAAT TGAGAATGGT
    GTAATTCTCA CAACGGACAC TCCACAAAAC TTGATTTCGG AATTCGGATC CCTTCAAAAA
    TGCCTTTTGA ACTTTTCGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.