let-99 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	let-99
Entrez Gene ID	178210
Full Name	Protein let-99
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_070006.4	NP_502407.1	Protein let-99
NM_070006.3	NP_502407.1	Protein let-99

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LET-99 acts in a pathway parallel to anillin and is required for myosin enrichment into the contractile ring. These and other results suggest a positive feedback model in which LET-99 localizes to the presumptive cleavage furrow in response to the spindle and myosin. LET-99 enhances myosin accumulation to promote furrowing in both symmetrically and asymmetrically dividing cells.
Title: LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring.

LET-99 acts in the Mes-1/Src-1 pathway for mitotic spindle positioning.
Title: Mitotic Spindle Positioning in the EMS Cell of Caenorhabditis elegans Requires LET-99 and LIN-5/NuMA.

Results indicate that PAR-5 protein acts in a mechanism with PAR-1 protein to regulate LET-99 protein cortical localization.
Title: The 14-3-3 protein PAR-5 regulates the asymmetric localization of the LET-99 spindle positioning protein.

The DEP domain protein LET-99 acts upstream of the heterotrimeric G protein alpha subunit GPA-11 to control activation of the stress kinase JNK-1.
Title: DEPDC1/LET-99 participates in an evolutionarily conserved pathway for anti-tubulin drug-induced apoptosis.

LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in C. elegans embryos
Title: LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in C. elegans embryos.

The following let-99 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the let-99 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa342524	NM_070006.4 Latest version!	Caenorhabditis elegans Protein let-99 (let-99), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OCa342524	NM_070006.3	Caenorhabditis elegans Protein let-99 (let-99), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

let-99 ( NM_070006.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_502407.1 Caenorhabditis elegans Protein let-99 (let-99), mRNA.

Price & Availability↑

CloneID	OCa342524
Clone ID Related Accession (Same CDS sequence)	NM_070006.4 , NM_070006.3
Accession Version	NM_070006.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein let-99
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_070006.3.

ATGTCTGCCG ACTACTCATC GGAGAAGTTC AAGGCGACGC ATCTGTTCGA CGAAATTCTA 
TGGCATTTTC GTTCAAACTT ATCACTCAAA ACGAACAGAA GAGGATTGGC CACTGCGGAA 
AACAGTTTCA GCGGGAAAGA AGCTGTGGAT TTTCTGATGA TTGAAATGCC ACGAATCATC 
CCTAACAATG TTCCGGAACG AGACAAGATG CAGAAGTTCC TGGAGTTCAT GATGGACATG 
AACGTGATTT CAGAAGCCTT CCCGAAGAAA GTCAAGCAAA GGAGGCCATT CAGTGAATCG 
AGAATATATT TGTTCATGAA GACTTTGGAT GAGTTGAAGC ATCCCAAGCC CCGATCACGT 
CGATCAGCGT CTTTCAGTGG TGCTCGCAAA AGTGCAAAAG TTGCTCAACC TGCCTCTCCT 
GCCGCGACAA TGCACCGGCC ACCAAAGGCA AGGCTCCCAC GAAGATTATC GCGATCTAAT 
GGCAACATTG ACAAAGCAGG CATCGATAAT TCTTCAGGCG GCGTTGAGAA TCACGGTTTC 
GATGATCATA AAGACGATGA AATCCCGAAG AAACAACGAA CTCCGAAGAT TTTGAACAGA 
TCACTTGAAA GTATCTGTAC AGAAGAATAT ACTGAAAAAC GAGAAGTTTC TGAGATGAAA 
GAGAAGGTTT ATGATTGGTT ACCGTTTTTC AAATCAAGAC GAAATCACAC GAAAGTCAAT 
CAACCAACCC GTCGTTCAGC ATCCCTTGAT CGAAATCATT GTGTTTTGGA ACAAGAAAAA 
GCTGAAGCTG CTCTTCGTAG TCAGAAAGTA ACCACTCCGC CAATTCGAGA AGCCCCGAAA 
GACGTCGTCT TTATGCATCC ACAAGGACCG TTGCCTGCAG TTCCTAGCAG ATATCAAAAT 
CATCGGACAA GCATCGCAGG ATCAAATCCA GCATTGTTAT CGCGTGGAAG AATGTACGAA 
TCCATCATGA GAAGATCGTC GGTTGTTCCA GTTGCAGACT CTGTCCAGGC AAACAACGAA 
TGCTCATTTT GGAAAACAGA GCTGCTTGGA CGATTGGAAC AAATTTACGA TCGCACTCTT 
CCCTGTGAAT GGGCATCTAA AGTCGATGGA TACGATATTC AGTGGAACAT GATTGAAATA 
GATCATGCTG ATGGAATCGT CAAATCGAGA TGTCAGGGAC TGCAGCCCGA TTATCCCCAA 
ACTGTTATTC AATTCATGGA TTATCTTGTT CGATATCCCT TCGTCACGCA TAAAAAAATG 
GACACAGGGT TGGAATACAA CGTCAATCGG ATTTTCATAA CTCTAGTCAA TCGTCTCGAA 
GACTTGAATG CTCCTCTTCA ATTCGATGAA TGCTCTCTTA TTGTTAATCT CCTCTGCAAA 
ATCGATAGCT TCGCGGCAAT GCTTGACAAT GGGCCTGCAA GGCGATGGTC GAAAGTGATG 
ATTTCTTCGT CTGCGTCTAG TATTGAGGAG GCTGGATTGA TGGTCGATGG ATTCTCCCGT 
GATCTTCCAG CATGCGGAAT TCGAGCTTCA AAGTATCGAC GACGTGCGTT ATCACCATTT 
GATAATAGAG TGAATTTGGA AATACAAGAC GAAAAATCCT ACGAAATCCG AGAGCAATGG 
TTGATCGAAG CAATTCAATT GGTACTTCTT TCGCTTCCAA CTTCACGACG TCGGAAACTT 
CACAAATTTG TTACATTCAT TCAATCGATC GAAACGAATG CAGTTTTCGA CCTCGCTGAT 
CCTTCTAACG GATCTTCCAA CAATCGGGAA GCGGCAATCA TCGGACTCTG GACAGGTGTT 
TGTAGTAAAT GCCGAAAGCA ACAAGGAATG CTGATAACCG CTGTCCTCCT CGCAAACTTT 
CAATCTCTTT TTGCAGTACC TGTTGAATTT ATCGAACAGG TCAAACGATT GGAATGTGAA 
GAAAAGGACA GATATAGTGG TCCTCGGTAC GCAAAGATCA CCAGAAGTCG AAATGATTGG 
CAACCACCAG TTCCGGATAA ACCGCAAGCA TCTCCGGCAG TTTTTAAGAA ACCACTAAGA 
GAAGTGGCAT GTGAGAAAAC GAAGAAAGGA TTGTTCACAA GATTGCTGCG TAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_502407.1
CDS	15..2111
Translation	MSADYSSEKFKATHLFDEILWHFRSNLSLKTNRRGLATAENSFSGKEAVDFLMIEMPRIIPNNVPERDKMQKFLEFMMDMNVISEAFPKKVKQRRPFSESRIYLFMKTLDELKHPKPRSRRSASFSGARKSAKVAQPASPAATMHRPPKARLPRRLSRSNGNIDKAGIDNSSGGVENHGFDDHKDDEIPKKQRTPKILNRSLESICTEEYTEKREVSEMKEKVYDWLPFFKSRRNHTKVNQPTRRSASLDRNHCVLEQEKAEAALRSQKVTTPPIREAPKDVVFMHPQGPLPAVPSRYQNHRTSIAGSNPALLSRGRMYESIMRRSSVVPVADSVQANNECSFWKTELLGRLEQIYDRTLPCEWASKVDGYDIQWNMIEIDHADGIVKSRCQGLQPDYPQTVIQFMDYLVRYPFVTHKKMDTGLEYNVNRIFITLVNRLEDLNAPLQFDECSLIVNLLCKIDSFAAMLDNGPARRWSKVMISSSASSIEEAGLMVDGFSRDLPACGIRASKYRRRALSPFDNRVNLEIQDEKSYEIREQWLIEAIQLVLLSLPTSRRRKLHKFVTFIQSIETNAVFDLADPSNGSSNNREAAIIGLWTGVCSKCRKQQGMLITAVLLANFQSLFAVPVEFIEQVKRLECEEKDRYSGPRYAKITRSRNDWQPPVPDKPQASPAVFKKPLREVACEKTKKGLFTRLLRK

Target ORF information:

RefSeq Version	NM_070006.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein let-99 (let-99), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_070006.4

ORF Insert Sequence:

ATGTCTGCCG ACTACTCATC GGAGAAGTTC AAGGCGACGC ATCTGTTCGA CGAAATTCTA 
TGGCATTTTC GTTCAAACTT ATCACTCAAA ACGAACAGAA GAGGATTGGC CACTGCGGAA 
AACAGTTTCA GCGGGAAAGA AGCTGTGGAT TTTCTGATGA TTGAAATGCC ACGAATCATC 
CCTAACAATG TTCCGGAACG AGACAAGATG CAGAAGTTCC TGGAGTTCAT GATGGACATG 
AACGTGATTT CAGAAGCCTT CCCGAAGAAA GTCAAGCAAA GGAGGCCATT CAGTGAATCG 
AGAATATATT TGTTCATGAA GACTTTGGAT GAGTTGAAGC ATCCCAAGCC CCGATCACGT 
CGATCAGCGT CTTTCAGTGG TGCTCGCAAA AGTGCAAAAG TTGCTCAACC TGCCTCTCCT 
GCCGCGACAA TGCACCGGCC ACCAAAGGCA AGGCTCCCAC GAAGATTATC GCGATCTAAT 
GGCAACATTG ACAAAGCAGG CATCGATAAT TCTTCAGGCG GCGTTGAGAA TCACGGTTTC 
GATGATCATA AAGACGATGA AATCCCGAAG AAACAACGAA CTCCGAAGAT TTTGAACAGA 
TCACTTGAAA GTATCTGTAC AGAAGAATAT ACTGAAAAAC GAGAAGTTTC TGAGATGAAA 
GAGAAGGTTT ATGATTGGTT ACCGTTTTTC AAATCAAGAC GAAATCACAC GAAAGTCAAT 
CAACCAACCC GTCGTTCAGC ATCCCTTGAT CGAAATCATT GTGTTTTGGA ACAAGAAAAA 
GCTGAAGCTG CTCTTCGTAG TCAGAAAGTA ACCACTCCGC CAATTCGAGA AGCCCCGAAA 
GACGTCGTCT TTATGCATCC ACAAGGACCG TTGCCTGCAG TTCCTAGCAG ATATCAAAAT 
CATCGGACAA GCATCGCAGG ATCAAATCCA GCATTGTTAT CGCGTGGAAG AATGTACGAA 
TCCATCATGA GAAGATCGTC GGTTGTTCCA GTTGCAGACT CTGTCCAGGC AAACAACGAA 
TGCTCATTTT GGAAAACAGA GCTGCTTGGA CGATTGGAAC AAATTTACGA TCGCACTCTT 
CCCTGTGAAT GGGCATCTAA AGTCGATGGA TACGATATTC AGTGGAACAT GATTGAAATA 
GATCATGCTG ATGGAATCGT CAAATCGAGA TGTCAGGGAC TGCAGCCCGA TTATCCCCAA 
ACTGTTATTC AATTCATGGA TTATCTTGTT CGATATCCCT TCGTCACGCA TAAAAAAATG 
GACACAGGGT TGGAATACAA CGTCAATCGG ATTTTCATAA CTCTAGTCAA TCGTCTCGAA 
GACTTGAATG CTCCTCTTCA ATTCGATGAA TGCTCTCTTA TTGTTAATCT CCTCTGCAAA 
ATCGATAGCT TCGCGGCAAT GCTTGACAAT GGGCCTGCAA GGCGATGGTC GAAAGTGATG 
ATTTCTTCGT CTGCGTCTAG TATTGAGGAG GCTGGATTGA TGGTCGATGG ATTCTCCCGT 
GATCTTCCAG CATGCGGAAT TCGAGCTTCA AAGTATCGAC GACGTGCGTT ATCACCATTT 
GATAATAGAG TGAATTTGGA AATACAAGAC GAAAAATCCT ACGAAATCCG AGAGCAATGG 
TTGATCGAAG CAATTCAATT GGTACTTCTT TCGCTTCCAA CTTCACGACG TCGGAAACTT 
CACAAATTTG TTACATTCAT TCAATCGATC GAAACGAATG CAGTTTTCGA CCTCGCTGAT 
CCTTCTAACG GATCTTCCAA CAATCGGGAA GCGGCAATCA TCGGACTCTG GACAGGTGTT 
TGTAGTAAAT GCCGAAAGCA ACAAGGAATG CTGATAACCG CTGTCCTCCT CGCAAACTTT 
CAATCTCTTT TTGCAGTACC TGTTGAATTT ATCGAACAGG TCAAACGATT GGAATGTGAA 
GAAAAGGACA GATATAGTGG TCCTCGGTAC GCAAAGATCA CCAGAAGTCG AAATGATTGG 
CAACCACCAG TTCCGGATAA ACCGCAAGCA TCTCCGGCAG TTTTTAAGAA ACCACTAAGA 
GAAGTGGCAT GTGAGAAAAC GAAGAAAGGA TTGTTCACAA GATTGCTGCG TAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

let-99 ( NM_070006.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_502407.1 Caenorhabditis elegans Protein let-99 (let-99), partial mRNA.

Price & Availability↑

CloneID	OCa342524
Clone ID Related Accession (Same CDS sequence)	NM_070006.4 , NM_070006.3
Accession Version	NM_070006.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Protein let-99
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_070006.2. COMPLETENESS: incomplete on both ends.

ATGTCTGCCG ACTACTCATC GGAGAAGTTC AAGGCGACGC ATCTGTTCGA CGAAATTCTA 
TGGCATTTTC GTTCAAACTT ATCACTCAAA ACGAACAGAA GAGGATTGGC CACTGCGGAA 
AACAGTTTCA GCGGGAAAGA AGCTGTGGAT TTTCTGATGA TTGAAATGCC ACGAATCATC 
CCTAACAATG TTCCGGAACG AGACAAGATG CAGAAGTTCC TGGAGTTCAT GATGGACATG 
AACGTGATTT CAGAAGCCTT CCCGAAGAAA GTCAAGCAAA GGAGGCCATT CAGTGAATCG 
AGAATATATT TGTTCATGAA GACTTTGGAT GAGTTGAAGC ATCCCAAGCC CCGATCACGT 
CGATCAGCGT CTTTCAGTGG TGCTCGCAAA AGTGCAAAAG TTGCTCAACC TGCCTCTCCT 
GCCGCGACAA TGCACCGGCC ACCAAAGGCA AGGCTCCCAC GAAGATTATC GCGATCTAAT 
GGCAACATTG ACAAAGCAGG CATCGATAAT TCTTCAGGCG GCGTTGAGAA TCACGGTTTC 
GATGATCATA AAGACGATGA AATCCCGAAG AAACAACGAA CTCCGAAGAT TTTGAACAGA 
TCACTTGAAA GTATCTGTAC AGAAGAATAT ACTGAAAAAC GAGAAGTTTC TGAGATGAAA 
GAGAAGGTTT ATGATTGGTT ACCGTTTTTC AAATCAAGAC GAAATCACAC GAAAGTCAAT 
CAACCAACCC GTCGTTCAGC ATCCCTTGAT CGAAATCATT GTGTTTTGGA ACAAGAAAAA 
GCTGAAGCTG CTCTTCGTAG TCAGAAAGTA ACCACTCCGC CAATTCGAGA AGCCCCGAAA 
GACGTCGTCT TTATGCATCC ACAAGGACCG TTGCCTGCAG TTCCTAGCAG ATATCAAAAT 
CATCGGACAA GCATCGCAGG ATCAAATCCA GCATTGTTAT CGCGTGGAAG AATGTACGAA 
TCCATCATGA GAAGATCGTC GGTTGTTCCA GTTGCAGACT CTGTCCAGGC AAACAACGAA 
TGCTCATTTT GGAAAACAGA GCTGCTTGGA CGATTGGAAC AAATTTACGA TCGCACTCTT 
CCCTGTGAAT GGGCATCTAA AGTCGATGGA TACGATATTC AGTGGAACAT GATTGAAATA 
GATCATGCTG ATGGAATCGT CAAATCGAGA TGTCAGGGAC TGCAGCCCGA TTATCCCCAA 
ACTGTTATTC AATTCATGGA TTATCTTGTT CGATATCCCT TCGTCACGCA TAAAAAAATG 
GACACAGGGT TGGAATACAA CGTCAATCGG ATTTTCATAA CTCTAGTCAA TCGTCTCGAA 
GACTTGAATG CTCCTCTTCA ATTCGATGAA TGCTCTCTTA TTGTTAATCT CCTCTGCAAA 
ATCGATAGCT TCGCGGCAAT GCTTGACAAT GGGCCTGCAA GGCGATGGTC GAAAGTGATG 
ATTTCTTCGT CTGCGTCTAG TATTGAGGAG GCTGGATTGA TGGTCGATGG ATTCTCCCGT 
GATCTTCCAG CATGCGGAAT TCGAGCTTCA AAGTATCGAC GACGTGCGTT ATCACCATTT 
GATAATAGAG TGAATTTGGA AATACAAGAC GAAAAATCCT ACGAAATCCG AGAGCAATGG 
TTGATCGAAG CAATTCAATT GGTACTTCTT TCGCTTCCAA CTTCACGACG TCGGAAACTT 
CACAAATTTG TTACATTCAT TCAATCGATC GAAACGAATG CAGTTTTCGA CCTCGCTGAT 
CCTTCTAACG GATCTTCCAA CAATCGGGAA GCGGCAATCA TCGGACTCTG GACAGGTGTT 
TGTAGTAAAT GCCGAAAGCA ACAAGGAATG CTGATAACCG CTGTCCTCCT CGCAAACTTT 
CAATCTCTTT TTGCAGTACC TGTTGAATTT ATCGAACAGG TCAAACGATT GGAATGTGAA 
GAAAAGGACA GATATAGTGG TCCTCGGTAC GCAAAGATCA CCAGAAGTCG AAATGATTGG 
CAACCACCAG TTCCGGATAA ACCGCAAGCA TCTCCGGCAG TTTTTAAGAA ACCACTAAGA 
GAAGTGGCAT GTGAGAAAAC GAAGAAAGGA TTGTTCACAA GATTGCTGCG TAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_502407.1
CDS	1..2097
Translation	MSADYSSEKFKATHLFDEILWHFRSNLSLKTNRRGLATAENSFSGKEAVDFLMIEMPRIIPNNVPERDKMQKFLEFMMDMNVISEAFPKKVKQRRPFSESRIYLFMKTLDELKHPKPRSRRSASFSGARKSAKVAQPASPAATMHRPPKARLPRRLSRSNGNIDKAGIDNSSGGVENHGFDDHKDDEIPKKQRTPKILNRSLESICTEEYTEKREVSEMKEKVYDWLPFFKSRRNHTKVNQPTRRSASLDRNHCVLEQEKAEAALRSQKVTTPPIREAPKDVVFMHPQGPLPAVPSRYQNHRTSIAGSNPALLSRGRMYESIMRRSSVVPVADSVQANNECSFWKTELLGRLEQIYDRTLPCEWASKVDGYDIQWNMIEIDHADGIVKSRCQGLQPDYPQTVIQFMDYLVRYPFVTHKKMDTGLEYNVNRIFITLVNRLEDLNAPLQFDECSLIVNLLCKIDSFAAMLDNGPARRWSKVMISSSASSIEEAGLMVDGFSRDLPACGIRASKYRRRALSPFDNRVNLEIQDEKSYEIREQWLIEAIQLVLLSLPTSRRRKLHKFVTFIQSIETNAVFDLADPSNGSSNNREAAIIGLWTGVCSKCRKQQGMLITAVLLANFQSLFAVPVEFIEQVKRLECEEKDRYSGPRYAKITRSRNDWQPPVPDKPQASPAVFKKPLREVACEKTKKGLFTRLLRK

Target ORF information:

RefSeq Version	NM_070006.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein let-99 (let-99), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_070006.3

ORF Insert Sequence:

ATGTCTGCCG ACTACTCATC GGAGAAGTTC AAGGCGACGC ATCTGTTCGA CGAAATTCTA 
TGGCATTTTC GTTCAAACTT ATCACTCAAA ACGAACAGAA GAGGATTGGC CACTGCGGAA 
AACAGTTTCA GCGGGAAAGA AGCTGTGGAT TTTCTGATGA TTGAAATGCC ACGAATCATC 
CCTAACAATG TTCCGGAACG AGACAAGATG CAGAAGTTCC TGGAGTTCAT GATGGACATG 
AACGTGATTT CAGAAGCCTT CCCGAAGAAA GTCAAGCAAA GGAGGCCATT CAGTGAATCG 
AGAATATATT TGTTCATGAA GACTTTGGAT GAGTTGAAGC ATCCCAAGCC CCGATCACGT 
CGATCAGCGT CTTTCAGTGG TGCTCGCAAA AGTGCAAAAG TTGCTCAACC TGCCTCTCCT 
GCCGCGACAA TGCACCGGCC ACCAAAGGCA AGGCTCCCAC GAAGATTATC GCGATCTAAT 
GGCAACATTG ACAAAGCAGG CATCGATAAT TCTTCAGGCG GCGTTGAGAA TCACGGTTTC 
GATGATCATA AAGACGATGA AATCCCGAAG AAACAACGAA CTCCGAAGAT TTTGAACAGA 
TCACTTGAAA GTATCTGTAC AGAAGAATAT ACTGAAAAAC GAGAAGTTTC TGAGATGAAA 
GAGAAGGTTT ATGATTGGTT ACCGTTTTTC AAATCAAGAC GAAATCACAC GAAAGTCAAT 
CAACCAACCC GTCGTTCAGC ATCCCTTGAT CGAAATCATT GTGTTTTGGA ACAAGAAAAA 
GCTGAAGCTG CTCTTCGTAG TCAGAAAGTA ACCACTCCGC CAATTCGAGA AGCCCCGAAA 
GACGTCGTCT TTATGCATCC ACAAGGACCG TTGCCTGCAG TTCCTAGCAG ATATCAAAAT 
CATCGGACAA GCATCGCAGG ATCAAATCCA GCATTGTTAT CGCGTGGAAG AATGTACGAA 
TCCATCATGA GAAGATCGTC GGTTGTTCCA GTTGCAGACT CTGTCCAGGC AAACAACGAA 
TGCTCATTTT GGAAAACAGA GCTGCTTGGA CGATTGGAAC AAATTTACGA TCGCACTCTT 
CCCTGTGAAT GGGCATCTAA AGTCGATGGA TACGATATTC AGTGGAACAT GATTGAAATA 
GATCATGCTG ATGGAATCGT CAAATCGAGA TGTCAGGGAC TGCAGCCCGA TTATCCCCAA 
ACTGTTATTC AATTCATGGA TTATCTTGTT CGATATCCCT TCGTCACGCA TAAAAAAATG 
GACACAGGGT TGGAATACAA CGTCAATCGG ATTTTCATAA CTCTAGTCAA TCGTCTCGAA 
GACTTGAATG CTCCTCTTCA ATTCGATGAA TGCTCTCTTA TTGTTAATCT CCTCTGCAAA 
ATCGATAGCT TCGCGGCAAT GCTTGACAAT GGGCCTGCAA GGCGATGGTC GAAAGTGATG 
ATTTCTTCGT CTGCGTCTAG TATTGAGGAG GCTGGATTGA TGGTCGATGG ATTCTCCCGT 
GATCTTCCAG CATGCGGAAT TCGAGCTTCA AAGTATCGAC GACGTGCGTT ATCACCATTT 
GATAATAGAG TGAATTTGGA AATACAAGAC GAAAAATCCT ACGAAATCCG AGAGCAATGG 
TTGATCGAAG CAATTCAATT GGTACTTCTT TCGCTTCCAA CTTCACGACG TCGGAAACTT 
CACAAATTTG TTACATTCAT TCAATCGATC GAAACGAATG CAGTTTTCGA CCTCGCTGAT 
CCTTCTAACG GATCTTCCAA CAATCGGGAA GCGGCAATCA TCGGACTCTG GACAGGTGTT 
TGTAGTAAAT GCCGAAAGCA ACAAGGAATG CTGATAACCG CTGTCCTCCT CGCAAACTTT 
CAATCTCTTT TTGCAGTACC TGTTGAATTT ATCGAACAGG TCAAACGATT GGAATGTGAA 
GAAAAGGACA GATATAGTGG TCCTCGGTAC GCAAAGATCA CCAGAAGTCG AAATGATTGG 
CAACCACCAG TTCCGGATAA ACCGCAAGCA TCTCCGGCAG TTTTTAAGAA ACCACTAAGA 
GAAGTGGCAT GTGAGAAAAC GAAGAAAGGA TTGTTCACAA GATTGCTGCG TAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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let-99 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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