dcaf-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	dcaf-1
Entrez Gene ID	177804
Full Name	DDB1 and CUL4-Associated Factor (DCAF1/VprBP) homolog
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001307074.1	NP_001294003.1	DDB1-and CUL4-associated factor homolog 1
NM_069324.5	NP_501725.2	DDB1-and CUL4-associated factor homolog 1
NM_069324.6	NP_501725.2	DDB1-and CUL4-associated factor homolog 1
NM_001307074.2	NP_001294003.1	DDB1-and CUL4-associated factor homolog 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	VPRBP	XP_005632545.1
Drosophila melanogaster (fruit fly)	mahj	NP_001246451.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012134	XP_320393.4
Mus musculus (house mouse)	Vprbp	NP_001015507.1
Rattus norvegicus (Norway rat)	Vprbp	NP_001178727.1
Gallus gallus (chicken)	VPRBP	XP_004944460.1
Xenopus tropicalis (tropical clawed frog)	vprbp	XP_004914132.1
Bos taurus (cattle)	VPRBP	XP_005223061.1
Caenorhabditis elegans (roundworm)	dcaf-1	NP_501725.2
Homo sapiens (human)	VPRBP	NP_055518.1
Pan troglodytes (chimpanzee)	VPRBP	XP_001169544.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dcaf-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dcaf-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa343020	NM_001307074.2 Latest version!	Caenorhabditis elegans DDB1-and CUL4-associated factor homolog 1 (dcaf-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OCa342205	NM_069324.6 Latest version!	Caenorhabditis elegans DDB1-and CUL4-associated factor homolog 1 (dcaf-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa342205	NM_069324.5	Caenorhabditis elegans DDB1-and CUL4-associated factor homolog 1 (dcaf-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCa343020	NM_001307074.1	Caenorhabditis elegans DDB1-and CUL4-associated factor homolog 1 (dcaf-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dcaf-1 ( NM_001307074.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294003.1 Caenorhabditis elegans DDB1-and CUL4-associated factor homolog 1 (dcaf-1), partial mRNA.

Price & Availability↑

CloneID	OCa343020
Clone ID Related Accession (Same CDS sequence)	NM_001307074.1 , NM_001307074.2
Accession Version	NM_001307074.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	DDB1-and CUL4-associated factor homolog 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGGGAGCGG CTATTGATGA TTTGGTAGAT GCAGTTGATG AAGAAGTTGA TGAAGATGAA 
TTGGGAACTG ATGGTGATGA TGATGATAGT GGAAGTTGGA GAACAACTAA TTCCATTGAC 
TCAGAAGACA TTAATCTAGA CGATCTCGAC GAAGAAGAAG CTCGTGTCGT GGAAAATGAG 
GGCAACAACG AACGCCCAGC GCGTCCAGTA GATCCAATAG AAGCAGCAGC TGCTGCTCGT 
CGAGCCATCC TCGGCCGCGG TCTCCGAGAT CTTCGAATGG GAATTAGAGG AGGTAATCGT 
CGTCGAAATG GAGGTGGTTT AGAGCAAGCT GAAATGTTGA ATGCCCGAGC TGAAGAGCAA 
CGAGTTTCGT TCATGGAGGC TCTTGTTAGA GGAGCTGAGG CTGAACGGAG AGAGGAAGAA 
GGTGGCGATG ACGGAGAAAG CAGTAGCAGC AGTAGTAGTG ATACTGATGA ATATCAATCC 
GAAGAGGAGG AGATCAACAG TGTCAGTACA ACCGCCCTCA ATCCAGCTCT GCGTCGAAGA 
AATCGTCGTC CTGACGATGA AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294003.1
CDS	1..567
Translation	MGAAIDDLVDAVDEEVDEDELGTDGDDDDSGSWRTTNSIDSEDINLDDLDEEEARVVENEGNNERPARPVDPIEAAAAARRAILGRGLRDLRMGIRGGNRRRNGGGLEQAEMLNARAEEQRVSFMEALVRGAEAERREEEGGDDGESSSSSSSDTDEYQSEEEEINSVSTTALNPALRRRNRRPDDEA

Target ORF information:

RefSeq Version	NM_001307074.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DDB1-and CUL4-associated factor homolog 1 (dcaf-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307074.1

ORF Insert Sequence:

ATGGGAGCGG CTATTGATGA TTTGGTAGAT GCAGTTGATG AAGAAGTTGA TGAAGATGAA 
TTGGGAACTG ATGGTGATGA TGATGATAGT GGAAGTTGGA GAACAACTAA TTCCATTGAC 
TCAGAAGACA TTAATCTAGA CGATCTCGAC GAAGAAGAAG CTCGTGTCGT GGAAAATGAG 
GGCAACAACG AACGCCCAGC GCGTCCAGTA GATCCAATAG AAGCAGCAGC TGCTGCTCGT 
CGAGCCATCC TCGGCCGCGG TCTCCGAGAT CTTCGAATGG GAATTAGAGG AGGTAATCGT 
CGTCGAAATG GAGGTGGTTT AGAGCAAGCT GAAATGTTGA ATGCCCGAGC TGAAGAGCAA 
CGAGTTTCGT TCATGGAGGC TCTTGTTAGA GGAGCTGAGG CTGAACGGAG AGAGGAAGAA 
GGTGGCGATG ACGGAGAAAG CAGTAGCAGC AGTAGTAGTG ATACTGATGA ATATCAATCC 
GAAGAGGAGG AGATCAACAG TGTCAGTACA ACCGCCCTCA ATCCAGCTCT GCGTCGAAGA 
AATCGTCGTC CTGACGATGA AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dcaf-1 ( NM_001307074.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294003.1 Caenorhabditis elegans DDB1-and CUL4-associated factor homolog 1 (dcaf-1), mRNA.

Price & Availability↑

CloneID	OCa343020
Clone ID Related Accession (Same CDS sequence)	NM_001307074.1 , NM_001307074.2
Accession Version	NM_001307074.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	DDB1-and CUL4-associated factor homolog 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307074.1.

ATGGGAGCGG CTATTGATGA TTTGGTAGAT GCAGTTGATG AAGAAGTTGA TGAAGATGAA 
TTGGGAACTG ATGGTGATGA TGATGATAGT GGAAGTTGGA GAACAACTAA TTCCATTGAC 
TCAGAAGACA TTAATCTAGA CGATCTCGAC GAAGAAGAAG CTCGTGTCGT GGAAAATGAG 
GGCAACAACG AACGCCCAGC GCGTCCAGTA GATCCAATAG AAGCAGCAGC TGCTGCTCGT 
CGAGCCATCC TCGGCCGCGG TCTCCGAGAT CTTCGAATGG GAATTAGAGG AGGTAATCGT 
CGTCGAAATG GAGGTGGTTT AGAGCAAGCT GAAATGTTGA ATGCCCGAGC TGAAGAGCAA 
CGAGTTTCGT TCATGGAGGC TCTTGTTAGA GGAGCTGAGG CTGAACGGAG AGAGGAAGAA 
GGTGGCGATG ACGGAGAAAG CAGTAGCAGC AGTAGTAGTG ATACTGATGA ATATCAATCC 
GAAGAGGAGG AGATCAACAG TGTCAGTACA ACCGCCCTCA ATCCAGCTCT GCGTCGAAGA 
AATCGTCGTC CTGACGATGA AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294003.1
CDS	4..570
Translation	MGAAIDDLVDAVDEEVDEDELGTDGDDDDSGSWRTTNSIDSEDINLDDLDEEEARVVENEGNNERPARPVDPIEAAAAARRAILGRGLRDLRMGIRGGNRRRNGGGLEQAEMLNARAEEQRVSFMEALVRGAEAERREEEGGDDGESSSSSSSDTDEYQSEEEEINSVSTTALNPALRRRNRRPDDEA

Target ORF information:

RefSeq Version	NM_001307074.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DDB1-and CUL4-associated factor homolog 1 (dcaf-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307074.2

ORF Insert Sequence:

ATGGGAGCGG CTATTGATGA TTTGGTAGAT GCAGTTGATG AAGAAGTTGA TGAAGATGAA 
TTGGGAACTG ATGGTGATGA TGATGATAGT GGAAGTTGGA GAACAACTAA TTCCATTGAC 
TCAGAAGACA TTAATCTAGA CGATCTCGAC GAAGAAGAAG CTCGTGTCGT GGAAAATGAG 
GGCAACAACG AACGCCCAGC GCGTCCAGTA GATCCAATAG AAGCAGCAGC TGCTGCTCGT 
CGAGCCATCC TCGGCCGCGG TCTCCGAGAT CTTCGAATGG GAATTAGAGG AGGTAATCGT 
CGTCGAAATG GAGGTGGTTT AGAGCAAGCT GAAATGTTGA ATGCCCGAGC TGAAGAGCAA 
CGAGTTTCGT TCATGGAGGC TCTTGTTAGA GGAGCTGAGG CTGAACGGAG AGAGGAAGAA 
GGTGGCGATG ACGGAGAAAG CAGTAGCAGC AGTAGTAGTG ATACTGATGA ATATCAATCC 
GAAGAGGAGG AGATCAACAG TGTCAGTACA ACCGCCCTCA ATCCAGCTCT GCGTCGAAGA 
AATCGTCGTC CTGACGATGA AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dcaf-1 ( NM_069324.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_501725.2 Caenorhabditis elegans DDB1-and CUL4-associated factor homolog 1 (dcaf-1), mRNA.

Price & Availability↑

CloneID	OCa342205
Clone ID Related Accession (Same CDS sequence)	NM_069324.5 , NM_069324.6
Accession Version	NM_069324.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	DDB1-and CUL4-associated factor homolog 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_069324.5.

ATGACGAGCA AGGAGATTTA TCCGCTCCGA TCGACAGGAA CAATGATGAT GATATCCGAT 
CAAGATTTGA AGCTGAGCAC GCAATTAATG CTTCGAATAG CTGAATTACT ATTGGAGTTT 
GATGCAAATC ATGAACAATC GTCGTTTGAT CCGATACCGA TACTTAAAAG AATTTCTGAA 
TTGTTGGAAC AAGCTACAGA CATTTTCATT AAAAACGACC CGGATCCTTT GGATGATCGT 
CATCCTCACA GGACTCATCC AGATTCTGCA CTCGGCAACA TTTTGAAAAT TATATTCAAA 
AACGATGATT TCATGACAAA ACTTGTTGTG TCGTATATTC TTGCCAGAGA CAACGTAGAA 
TTGAATATTC AAGGAAGTCG ACTTCTGTTA GCCTGTATTC CTGGTCTCGA TTCAAAAGTA 
GTTTTCTCAG AACCAGACGA TTTCATTCCT CGCCTATACA CATGGGCAGG ATCAGAAGGA 
ACAAATGAGA CTTTACAAGG TTATGCAATG GGACTTCTAG CTGCTGCATT GGAAAATACT 
GAAAATGCGA GTAAATATCG AAATGAAAAT GCTCTATTAG TCCCATTTGG ACTTCGGCGA 
CTCCATGAAT TACAGGGTCG CTCTTTGGAA GAACAGAAGA AAATTGGACA GACTGATTTC 
TCCCAACTGC ATGCTGAACA AAGTACAAGT AACGGAACTT CAATTCCTTC AATCAAAATC 
ACTTCTGTTG ATGGTTCTAC AAAAGAGAAT GAGAAAACCT TTGTACAATC AACAGATCCA 
CCACCACCGA AAAAGCGAAG AACTGAGCCT TGCCTGACAT CTCTTCTTCG AACGGAAATT 
ACTCAGCGAG TTCCATCATT TCATAATCTT CGAAATTTGG ATGATTCAAA CAGTAAATGG 
GATATTCTTC AACCATTTCT AATTGGAGAT CAACAAGTAT ATCCACTTAG TTTAGCTACA 
TATCAACGGT TTATTCTACA ATATTTGGCT GCATGTGGAG AATATCAGGA TCTCCTCCTT 
CAAACTTTTG AAGGAAATGC ATTGGAAATC TTGTTCGACT ACATTGATCT CGAAAAATCA 
AAAGATGTCC GTTTAACCTT TGATGCTTTA AAATATCTCA CTTCTCTATT GGTACATCGT 
AAATTCGCCT TGGAATTCGT CAACAAAGGA GGAATACCGG CTCTTTTGAA AGTTCCAAAA 
ACATCATTGG CTTCAGTTGG AGTTGTTACA TGTCTGTATT ACATTGCATA CAGTAATGAT 
GTCATGGAGA TTTTATGTCA AATGAGCGAT GAGATTGTGG ATGAGACTGT ACAGTATGTT 
CTGTGGTGCT TGGAACACAG TCATGAAAGT GGAATGGCCA GTGCATGTAT GTTCTTTTCT 
CAAGGATTGT TCTACAAGGC TATTTTAAGA AGATTTGATC AATATGATGG TCCACGGAAG 
CTGCATAATT ATATTGCAAC CCTGACACTA ATGCAAAACA ATGAAGATGT CGAACTCACC 
GAAGAACAAA TACATACAAG TACACAGTGT ACAAGAGGAG TTTGTACCAC TTTCAGAAGT 
TACCTCACCG CTCACATTTT CATCAAAGTG GAAAACTATA AAAAGCTATA CGGAAATAAC 
TTACCAACTG GAATGCGATT TCCAGAACTT GTACAAGGAG ATTGTCCAGA TTATAAAAGC 
ATGAAGCCGT ATGAAGAAGT TTGTTGGCAG TGCGAAGCCA TTGTTACGGA AATGCTTCGA 
TTCACCGGAA GTTCATTCAG AGAGGCTGAA AATTTAAGAA AACTAGGAAT GGTTCGAATG 
TTTTTGGCGG TTAGAGTGTT ATCAAGAGAT TGGGAAAACA TTTCTCCAAG TCTCCGCACC 
GAAATGTGTG TACACGCATT GGAAACATTG TGCATGATGT TTTGCCTTCC ATCAATTCAA 
ACTGAACTTA TCACTCAACA TTCCTATAAT CACTCGAATT ATGATGGTTT CACAATTCTT 
CTTCAAACAT CTCTTGGACG ATACGATGAG GATCCATCTC TGAGAATGGC AGCATTGGGA 
TGTATTCAAC GTTGTGTTTA TGTGGAACCA GAATGCTGGA AAGCTATTAT TCAAAGAGTC 
AAAAGTTCAG AGGAAAAGTC AAGCTCCGCG TCGAAAAGAC AATCGAAATA TGAAATAATC 
ATGAATCATC TTGAACGAAT GTGGACAGAA GTTCGAAAAA CTGATGGAAT TATGGCACTC 
GTCAACTTGA TTAACTGCAA AATTCCACTG ACAGAAGCGG ATTCTATCCG AAAAACGGCA 
ACTAACACAT TAACGGGACT GGCAAGGCAT CCGGAAGTTC GACAGATTCT TGCAAAATTA 
CCGTTGATAG CTCATAATGG ACTACAGAAT TTAATGCGAG AACCAGTGTG TTCTGACAAG 
CGAGATATTC ATGCAGCATT CTGCAAAGAA GCAGTTCAAC TTCTTCAGGT CATTTATGGA 
AGAAAAATTC ATGATCAACA AGGAAAAGAA ATTCAATCGT CGGAAAAGAG TCACCGTCAA 
TGGGTCATCG AAAATACACA AGTGAGCTTC AATCAAGCAG AACTTCTTCA ATTAATTCAT 
GATCACTTGT TGAAAAGCAA GTTAGATTCA GTAGCAGCAA TGCTGAAATC TGAAGCAAAG 
CTTCCTGATC GTCCAGCAAG TCGGTCAATC AATACTCCGA TTCTCAATAA GCCATTGCCC 
AGCAGCGGAA ATAATTTTTC AAAAATAAAC GACACTTATC CAACATTGGC TCCAAGGACG 
CTTGAATCGG AAATTGGTGG AATTTCAGCA AGACGGCCCT CAAACGCAGC TTCACTGTCA 
TCTCCCGCAA TGGCTACACG TTCTCATTCA ACAGATGACG ATGTTTTCGC CACACCAACT 
TTACCTAGAA GATATACAAC CAGTGGTGCT TTTCCAAAGA AGTTGATGAT CAGTCCTGCA 
CGTCAAAAAC TGAGACCTCT GACTCCAGGA GAAAGTTCAA GTGGATATCG ACCAATTAAA 
GATCTAAACA GTATTGTTAC TGATTATTTC AGAAACCAAC ATTCAACATG TAAGAATCCA 
GTGACAACGT GTCCTCCATT TTCATTATTT TACCAACACA AATGTCCCGA ATTGTCGTAT 
CAAACGAATG TTGTGAGAAA TATTTCTCTT CGAACACTCG ATCAAGAACT TCTTAGACCT 
CATGAAAGAG TTTATTCACA GTGGACAAAT GAAAGAACTA TTTTCTCAAG ATTCAGGAAC 
TGGAAAACTA TACATGATCA CGATGAATCG TATACGAAAG CCACATTTTC GGTCGACGAT 
GAACATCTTA TCGTTGGTTT ATTCAATGGA GAAGTTCATT GGATAAACGT GGATACTGGA 
TTGGATGAAG GACACACCAA TTGCCATGGA AGTGCTCTGA CGAATATTGA ACCTTCGAAG 
GATGGTTCAA TGATGCTCAC GTCTTCAGCA TTCTCTCGTC CACTATCCGC TCTTTGGAGA 
CTCGGAGAAG CTTTACAACG AGTTCATACT TATCGTGAAG ATTCTTGTGT TAAATTTGCT 
AACACAACGA TGCAAAGAAT TGTCGGAACG TGTAGAGATA AGGCAACTGT CTATGACACT 
GAAACAAATC ATGTTTTGGA CACGTATCTC TCTGGAATAG ATGGACTACA GTACGAGAAA 
AATTACGCTT CGTTTTCGCC TGATGACAAG CTAATCTTCA ATGATGGACT TCTCTGGGAT 
GTCAGAAAGA AGAATTCGGC GATCCATGTA TTTGATCGTC TCTCCAAAAT AACATTATTC 
GGAACATTCC ATCCTCATGG AACACAGATT GTTATAAATT CTGAAGTTTA TGATATTCGT 
ACATTCCGGA TGCTTCACCA TGTTCCTGAG CTCAATCGTT GTCAAGTTTC ATTTAATTCC 
ACTGGTAATA TCATGTACGC CACTGAAGTT ACAGACGTTC ATTGTCCAGA CTATGATGAA 
AAGATCTTCT CGTCATTCCG AACGTTCGAG ACCCGTGACT ATTCTGCTCT GACAACTTTC 
GAAGGACGAC GACCTGTCAT TGATCTATGC GCATCACATC AAGATCAGAA AATGTGTGTG 
ATTGAGAAAG TGCGTCCACA AATGTCGGAT TACATGATAC AAGCATCTAC ACAACTGAAA 
ATAGTTGAAA TTGGACGATT GAAGGATAAT GAAGATGAGA ATGACGAAGA GGAAGATGAA 
CAGAGAGAGG ATCACGATGA AGACGAAGAT TCGGATGAAT CTGGAGATGG AGATGATGAT 
GAAGAGATTG GAGGAAATAG TTCAGATCGA GAATCCGTGT TTCGAACTTT AGGAAGACTT 
GGCGATGAAA GTGACAGTGG AAGTAGCGTG GATGACAATG ATACACTAGA CGATTTGGAC 
TTTGAAAATG CACAGAATCG GATTATCAGA CGTCAAGCTC AACGCCGTCG TCAACGCCTA 
AACAGTAGTG AAAATGATGC AGAGCTTCCA GGTTCTGATG AAGGAAGTGA TGAAGATGGA 
GATGATGATG AGGATGGAGA AGGGGATCCA GATTTTGATA TGGGAGCGGC TATTGATGAT 
TTGGTAGATG CAGTTGATGA AGAAGTTGAT GAAGATGAAT TGGGAACTGA TGGTGATGAT 
GATGATAGTG GAAGTTGGAG AACAACTAAT TCCATTGACT CAGAAGACAT TAATCTAGAC 
GATCTCGACG AAGAAGAAGC TCGTGTCGTG GAAAATGAGG GCAACAACGA ACGCCCAGCG 
CGTCCAGTAG ATCCAATAGA AGCAGCAGCT GCTGCTCGTC GAGCCATCCT CGGCCGCGGT 
CTCCGAGATC TTCGAATGGG AATTAGAGGA GGTAATCGTC GTCGAAATGG AGGTGGTTTA 
GAGCAAGCTG AAATGTTGAA TGCCCGAGCT GAAGAGCAAC GAGTTTCGTT CATGGAGGCT 
CTTGTTAGAG GAGCTGAGGC TGAACGGAGA GAGGAAGAAG GTGGCGATGA CGGAGAAAGC 
AGTAGCAGCA GTAGTAGTGA TACTGATGAA TATCAATCCG AAGAGGAGGA GATCAACAGT 
GTCAGTACAA CCGCCCTCAA TCCAGCTCTG CGTCGAAGAA ATCGTCGTCC TGACGATGAA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_501725.2
CDS	6..5111
Translation	MTSKEIYPLRSTGTMMMISDQDLKLSTQLMLRIAELLLEFDANHEQSSFDPIPILKRISELLEQATDIFIKNDPDPLDDRHPHRTHPDSALGNILKIIFKNDDFMTKLVVSYILARDNVELNIQGSRLLLACIPGLDSKVVFSEPDDFIPRLYTWAGSEGTNETLQGYAMGLLAAALENTENASKYRNENALLVPFGLRRLHELQGRSLEEQKKIGQTDFSQLHAEQSTSNGTSIPSIKITSVDGSTKENEKTFVQSTDPPPPKKRRTEPCLTSLLRTEITQRVPSFHNLRNLDDSNSKWDILQPFLIGDQQVYPLSLATYQRFILQYLAACGEYQDLLLQTFEGNALEILFDYIDLEKSKDVRLTFDALKYLTSLLVHRKFALEFVNKGGIPALLKVPKTSLASVGVVTCLYYIAYSNDVMEILCQMSDEIVDETVQYVLWCLEHSHESGMASACMFFSQGLFYKAILRRFDQYDGPRKLHNYIATLTLMQNNEDVELTEEQIHTSTQCTRGVCTTFRSYLTAHIFIKVENYKKLYGNNLPTGMRFPELVQGDCPDYKSMKPYEEVCWQCEAIVTEMLRFTGSSFREAENLRKLGMVRMFLAVRVLSRDWENISPSLRTEMCVHALETLCMMFCLPSIQTELITQHSYNHSNYDGFTILLQTSLGRYDEDPSLRMAALGCIQRCVYVEPECWKAIIQRVKSSEEKSSSASKRQSKYEIIMNHLERMWTEVRKTDGIMALVNLINCKIPLTEADSIRKTATNTLTGLARHPEVRQILAKLPLIAHNGLQNLMREPVCSDKRDIHAAFCKEAVQLLQVIYGRKIHDQQGKEIQSSEKSHRQWVIENTQVSFNQAELLQLIHDHLLKSKLDSVAAMLKSEAKLPDRPASRSINTPILNKPLPSSGNNFSKINDTYPTLAPRTLESEIGGISARRPSNAASLSSPAMATRSHSTDDDVFATPTLPRRYTTSGAFPKKLMISPARQKLRPLTPGESSSGYRPIKDLNSIVTDYFRNQHSTCKNPVTTCPPFSLFYQHKCPELSYQTNVVRNISLRTLDQELLRPHERVYSQWTNERTIFSRFRNWKTIHDHDESYTKATFSVDDEHLIVGLFNGEVHWINVDTGLDEGHTNCHGSALTNIEPSKDGSMMLTSSAFSRPLSALWRLGEALQRVHTYREDSCVKFANTTMQRIVGTCRDKATVYDTETNHVLDTYLSGIDGLQYEKNYASFSPDDKLIFNDGLLWDVRKKNSAIHVFDRLSKITLFGTFHPHGTQIVINSEVYDIRTFRMLHHVPELNRCQVSFNSTGNIMYATEVTDVHCPDYDEKIFSSFRTFETRDYSALTTFEGRRPVIDLCASHQDQKMCVIEKVRPQMSDYMIQASTQLKIVEIGRLKDNEDENDEEEDEQREDHDEDEDSDESGDGDDDEEIGGNSSDRESVFRTLGRLGDESDSGSSVDDNDTLDDLDFENAQNRIIRRQAQRRRQRLNSSENDAELPGSDEGSDEDGDDDEDGEGDPDFDMGAAIDDLVDAVDEEVDEDELGTDGDDDDSGSWRTTNSIDSEDINLDDLDEEEARVVENEGNNERPARPVDPIEAAAAARRAILGRGLRDLRMGIRGGNRRRNGGGLEQAEMLNARAEEQRVSFMEALVRGAEAERREEEGGDDGESSSSSSSDTDEYQSEEEEINSVSTTALNPALRRRNRRPDDEA

Target ORF information:

RefSeq Version	NM_069324.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DDB1-and CUL4-associated factor homolog 1 (dcaf-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069324.6

ORF Insert Sequence:

ATGACGAGCA AGGAGATTTA TCCGCTCCGA TCGACAGGAA CAATGATGAT GATATCCGAT 
CAAGATTTGA AGCTGAGCAC GCAATTAATG CTTCGAATAG CTGAATTACT ATTGGAGTTT 
GATGCAAATC ATGAACAATC GTCGTTTGAT CCGATACCGA TACTTAAAAG AATTTCTGAA 
TTGTTGGAAC AAGCTACAGA CATTTTCATT AAAAACGACC CGGATCCTTT GGATGATCGT 
CATCCTCACA GGACTCATCC AGATTCTGCA CTCGGCAACA TTTTGAAAAT TATATTCAAA 
AACGATGATT TCATGACAAA ACTTGTTGTG TCGTATATTC TTGCCAGAGA CAACGTAGAA 
TTGAATATTC AAGGAAGTCG ACTTCTGTTA GCCTGTATTC CTGGTCTCGA TTCAAAAGTA 
GTTTTCTCAG AACCAGACGA TTTCATTCCT CGCCTATACA CATGGGCAGG ATCAGAAGGA 
ACAAATGAGA CTTTACAAGG TTATGCAATG GGACTTCTAG CTGCTGCATT GGAAAATACT 
GAAAATGCGA GTAAATATCG AAATGAAAAT GCTCTATTAG TCCCATTTGG ACTTCGGCGA 
CTCCATGAAT TACAGGGTCG CTCTTTGGAA GAACAGAAGA AAATTGGACA GACTGATTTC 
TCCCAACTGC ATGCTGAACA AAGTACAAGT AACGGAACTT CAATTCCTTC AATCAAAATC 
ACTTCTGTTG ATGGTTCTAC AAAAGAGAAT GAGAAAACCT TTGTACAATC AACAGATCCA 
CCACCACCGA AAAAGCGAAG AACTGAGCCT TGCCTGACAT CTCTTCTTCG AACGGAAATT 
ACTCAGCGAG TTCCATCATT TCATAATCTT CGAAATTTGG ATGATTCAAA CAGTAAATGG 
GATATTCTTC AACCATTTCT AATTGGAGAT CAACAAGTAT ATCCACTTAG TTTAGCTACA 
TATCAACGGT TTATTCTACA ATATTTGGCT GCATGTGGAG AATATCAGGA TCTCCTCCTT 
CAAACTTTTG AAGGAAATGC ATTGGAAATC TTGTTCGACT ACATTGATCT CGAAAAATCA 
AAAGATGTCC GTTTAACCTT TGATGCTTTA AAATATCTCA CTTCTCTATT GGTACATCGT 
AAATTCGCCT TGGAATTCGT CAACAAAGGA GGAATACCGG CTCTTTTGAA AGTTCCAAAA 
ACATCATTGG CTTCAGTTGG AGTTGTTACA TGTCTGTATT ACATTGCATA CAGTAATGAT 
GTCATGGAGA TTTTATGTCA AATGAGCGAT GAGATTGTGG ATGAGACTGT ACAGTATGTT 
CTGTGGTGCT TGGAACACAG TCATGAAAGT GGAATGGCCA GTGCATGTAT GTTCTTTTCT 
CAAGGATTGT TCTACAAGGC TATTTTAAGA AGATTTGATC AATATGATGG TCCACGGAAG 
CTGCATAATT ATATTGCAAC CCTGACACTA ATGCAAAACA ATGAAGATGT CGAACTCACC 
GAAGAACAAA TACATACAAG TACACAGTGT ACAAGAGGAG TTTGTACCAC TTTCAGAAGT 
TACCTCACCG CTCACATTTT CATCAAAGTG GAAAACTATA AAAAGCTATA CGGAAATAAC 
TTACCAACTG GAATGCGATT TCCAGAACTT GTACAAGGAG ATTGTCCAGA TTATAAAAGC 
ATGAAGCCGT ATGAAGAAGT TTGTTGGCAG TGCGAAGCCA TTGTTACGGA AATGCTTCGA 
TTCACCGGAA GTTCATTCAG AGAGGCTGAA AATTTAAGAA AACTAGGAAT GGTTCGAATG 
TTTTTGGCGG TTAGAGTGTT ATCAAGAGAT TGGGAAAACA TTTCTCCAAG TCTCCGCACC 
GAAATGTGTG TACACGCATT GGAAACATTG TGCATGATGT TTTGCCTTCC ATCAATTCAA 
ACTGAACTTA TCACTCAACA TTCCTATAAT CACTCGAATT ATGATGGTTT CACAATTCTT 
CTTCAAACAT CTCTTGGACG ATACGATGAG GATCCATCTC TGAGAATGGC AGCATTGGGA 
TGTATTCAAC GTTGTGTTTA TGTGGAACCA GAATGCTGGA AAGCTATTAT TCAAAGAGTC 
AAAAGTTCAG AGGAAAAGTC AAGCTCCGCG TCGAAAAGAC AATCGAAATA TGAAATAATC 
ATGAATCATC TTGAACGAAT GTGGACAGAA GTTCGAAAAA CTGATGGAAT TATGGCACTC 
GTCAACTTGA TTAACTGCAA AATTCCACTG ACAGAAGCGG ATTCTATCCG AAAAACGGCA 
ACTAACACAT TAACGGGACT GGCAAGGCAT CCGGAAGTTC GACAGATTCT TGCAAAATTA 
CCGTTGATAG CTCATAATGG ACTACAGAAT TTAATGCGAG AACCAGTGTG TTCTGACAAG 
CGAGATATTC ATGCAGCATT CTGCAAAGAA GCAGTTCAAC TTCTTCAGGT CATTTATGGA 
AGAAAAATTC ATGATCAACA AGGAAAAGAA ATTCAATCGT CGGAAAAGAG TCACCGTCAA 
TGGGTCATCG AAAATACACA AGTGAGCTTC AATCAAGCAG AACTTCTTCA ATTAATTCAT 
GATCACTTGT TGAAAAGCAA GTTAGATTCA GTAGCAGCAA TGCTGAAATC TGAAGCAAAG 
CTTCCTGATC GTCCAGCAAG TCGGTCAATC AATACTCCGA TTCTCAATAA GCCATTGCCC 
AGCAGCGGAA ATAATTTTTC AAAAATAAAC GACACTTATC CAACATTGGC TCCAAGGACG 
CTTGAATCGG AAATTGGTGG AATTTCAGCA AGACGGCCCT CAAACGCAGC TTCACTGTCA 
TCTCCCGCAA TGGCTACACG TTCTCATTCA ACAGATGACG ATGTTTTCGC CACACCAACT 
TTACCTAGAA GATATACAAC CAGTGGTGCT TTTCCAAAGA AGTTGATGAT CAGTCCTGCA 
CGTCAAAAAC TGAGACCTCT GACTCCAGGA GAAAGTTCAA GTGGATATCG ACCAATTAAA 
GATCTAAACA GTATTGTTAC TGATTATTTC AGAAACCAAC ATTCAACATG TAAGAATCCA 
GTGACAACGT GTCCTCCATT TTCATTATTT TACCAACACA AATGTCCCGA ATTGTCGTAT 
CAAACGAATG TTGTGAGAAA TATTTCTCTT CGAACACTCG ATCAAGAACT TCTTAGACCT 
CATGAAAGAG TTTATTCACA GTGGACAAAT GAAAGAACTA TTTTCTCAAG ATTCAGGAAC 
TGGAAAACTA TACATGATCA CGATGAATCG TATACGAAAG CCACATTTTC GGTCGACGAT 
GAACATCTTA TCGTTGGTTT ATTCAATGGA GAAGTTCATT GGATAAACGT GGATACTGGA 
TTGGATGAAG GACACACCAA TTGCCATGGA AGTGCTCTGA CGAATATTGA ACCTTCGAAG 
GATGGTTCAA TGATGCTCAC GTCTTCAGCA TTCTCTCGTC CACTATCCGC TCTTTGGAGA 
CTCGGAGAAG CTTTACAACG AGTTCATACT TATCGTGAAG ATTCTTGTGT TAAATTTGCT 
AACACAACGA TGCAAAGAAT TGTCGGAACG TGTAGAGATA AGGCAACTGT CTATGACACT 
GAAACAAATC ATGTTTTGGA CACGTATCTC TCTGGAATAG ATGGACTACA GTACGAGAAA 
AATTACGCTT CGTTTTCGCC TGATGACAAG CTAATCTTCA ATGATGGACT TCTCTGGGAT 
GTCAGAAAGA AGAATTCGGC GATCCATGTA TTTGATCGTC TCTCCAAAAT AACATTATTC 
GGAACATTCC ATCCTCATGG AACACAGATT GTTATAAATT CTGAAGTTTA TGATATTCGT 
ACATTCCGGA TGCTTCACCA TGTTCCTGAG CTCAATCGTT GTCAAGTTTC ATTTAATTCC 
ACTGGTAATA TCATGTACGC CACTGAAGTT ACAGACGTTC ATTGTCCAGA CTATGATGAA 
AAGATCTTCT CGTCATTCCG AACGTTCGAG ACCCGTGACT ATTCTGCTCT GACAACTTTC 
GAAGGACGAC GACCTGTCAT TGATCTATGC GCATCACATC AAGATCAGAA AATGTGTGTG 
ATTGAGAAAG TGCGTCCACA AATGTCGGAT TACATGATAC AAGCATCTAC ACAACTGAAA 
ATAGTTGAAA TTGGACGATT GAAGGATAAT GAAGATGAGA ATGACGAAGA GGAAGATGAA 
CAGAGAGAGG ATCACGATGA AGACGAAGAT TCGGATGAAT CTGGAGATGG AGATGATGAT 
GAAGAGATTG GAGGAAATAG TTCAGATCGA GAATCCGTGT TTCGAACTTT AGGAAGACTT 
GGCGATGAAA GTGACAGTGG AAGTAGCGTG GATGACAATG ATACACTAGA CGATTTGGAC 
TTTGAAAATG CACAGAATCG GATTATCAGA CGTCAAGCTC AACGCCGTCG TCAACGCCTA 
AACAGTAGTG AAAATGATGC AGAGCTTCCA GGTTCTGATG AAGGAAGTGA TGAAGATGGA 
GATGATGATG AGGATGGAGA AGGGGATCCA GATTTTGATA TGGGAGCGGC TATTGATGAT 
TTGGTAGATG CAGTTGATGA AGAAGTTGAT GAAGATGAAT TGGGAACTGA TGGTGATGAT 
GATGATAGTG GAAGTTGGAG AACAACTAAT TCCATTGACT CAGAAGACAT TAATCTAGAC 
GATCTCGACG AAGAAGAAGC TCGTGTCGTG GAAAATGAGG GCAACAACGA ACGCCCAGCG 
CGTCCAGTAG ATCCAATAGA AGCAGCAGCT GCTGCTCGTC GAGCCATCCT CGGCCGCGGT 
CTCCGAGATC TTCGAATGGG AATTAGAGGA GGTAATCGTC GTCGAAATGG AGGTGGTTTA 
GAGCAAGCTG AAATGTTGAA TGCCCGAGCT GAAGAGCAAC GAGTTTCGTT CATGGAGGCT 
CTTGTTAGAG GAGCTGAGGC TGAACGGAGA GAGGAAGAAG GTGGCGATGA CGGAGAAAGC 
AGTAGCAGCA GTAGTAGTGA TACTGATGAA TATCAATCCG AAGAGGAGGA GATCAACAGT 
GTCAGTACAA CCGCCCTCAA TCCAGCTCTG CGTCGAAGAA ATCGTCGTCC TGACGATGAA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dcaf-1 ( NM_069324.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_501725.2 Caenorhabditis elegans DDB1-and CUL4-associated factor homolog 1 (dcaf-1), partial mRNA.

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CloneID	OCa342205
Clone ID Related Accession (Same CDS sequence)	NM_069324.5 , NM_069324.6
Accession Version	NM_069324.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	DDB1-and CUL4-associated factor homolog 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_069324.4. COMPLETENESS: incomplete on both ends.

ATGACGAGCA AGGAGATTTA TCCGCTCCGA TCGACAGGAA CAATGATGAT GATATCCGAT 
CAAGATTTGA AGCTGAGCAC GCAATTAATG CTTCGAATAG CTGAATTACT ATTGGAGTTT 
GATGCAAATC ATGAACAATC GTCGTTTGAT CCGATACCGA TACTTAAAAG AATTTCTGAA 
TTGTTGGAAC AAGCTACAGA CATTTTCATT AAAAACGACC CGGATCCTTT GGATGATCGT 
CATCCTCACA GGACTCATCC AGATTCTGCA CTCGGCAACA TTTTGAAAAT TATATTCAAA 
AACGATGATT TCATGACAAA ACTTGTTGTG TCGTATATTC TTGCCAGAGA CAACGTAGAA 
TTGAATATTC AAGGAAGTCG ACTTCTGTTA GCCTGTATTC CTGGTCTCGA TTCAAAAGTA 
GTTTTCTCAG AACCAGACGA TTTCATTCCT CGCCTATACA CATGGGCAGG ATCAGAAGGA 
ACAAATGAGA CTTTACAAGG TTATGCAATG GGACTTCTAG CTGCTGCATT GGAAAATACT 
GAAAATGCGA GTAAATATCG AAATGAAAAT GCTCTATTAG TCCCATTTGG ACTTCGGCGA 
CTCCATGAAT TACAGGGTCG CTCTTTGGAA GAACAGAAGA AAATTGGACA GACTGATTTC 
TCCCAACTGC ATGCTGAACA AAGTACAAGT AACGGAACTT CAATTCCTTC AATCAAAATC 
ACTTCTGTTG ATGGTTCTAC AAAAGAGAAT GAGAAAACCT TTGTACAATC AACAGATCCA 
CCACCACCGA AAAAGCGAAG AACTGAGCCT TGCCTGACAT CTCTTCTTCG AACGGAAATT 
ACTCAGCGAG TTCCATCATT TCATAATCTT CGAAATTTGG ATGATTCAAA CAGTAAATGG 
GATATTCTTC AACCATTTCT AATTGGAGAT CAACAAGTAT ATCCACTTAG TTTAGCTACA 
TATCAACGGT TTATTCTACA ATATTTGGCT GCATGTGGAG AATATCAGGA TCTCCTCCTT 
CAAACTTTTG AAGGAAATGC ATTGGAAATC TTGTTCGACT ACATTGATCT CGAAAAATCA 
AAAGATGTCC GTTTAACCTT TGATGCTTTA AAATATCTCA CTTCTCTATT GGTACATCGT 
AAATTCGCCT TGGAATTCGT CAACAAAGGA GGAATACCGG CTCTTTTGAA AGTTCCAAAA 
ACATCATTGG CTTCAGTTGG AGTTGTTACA TGTCTGTATT ACATTGCATA CAGTAATGAT 
GTCATGGAGA TTTTATGTCA AATGAGCGAT GAGATTGTGG ATGAGACTGT ACAGTATGTT 
CTGTGGTGCT TGGAACACAG TCATGAAAGT GGAATGGCCA GTGCATGTAT GTTCTTTTCT 
CAAGGATTGT TCTACAAGGC TATTTTAAGA AGATTTGATC AATATGATGG TCCACGGAAG 
CTGCATAATT ATATTGCAAC CCTGACACTA ATGCAAAACA ATGAAGATGT CGAACTCACC 
GAAGAACAAA TACATACAAG TACACAGTGT ACAAGAGGAG TTTGTACCAC TTTCAGAAGT 
TACCTCACCG CTCACATTTT CATCAAAGTG GAAAACTATA AAAAGCTATA CGGAAATAAC 
TTACCAACTG GAATGCGATT TCCAGAACTT GTACAAGGAG ATTGTCCAGA TTATAAAAGC 
ATGAAGCCGT ATGAAGAAGT TTGTTGGCAG TGCGAAGCCA TTGTTACGGA AATGCTTCGA 
TTCACCGGAA GTTCATTCAG AGAGGCTGAA AATTTAAGAA AACTAGGAAT GGTTCGAATG 
TTTTTGGCGG TTAGAGTGTT ATCAAGAGAT TGGGAAAACA TTTCTCCAAG TCTCCGCACC 
GAAATGTGTG TACACGCATT GGAAACATTG TGCATGATGT TTTGCCTTCC ATCAATTCAA 
ACTGAACTTA TCACTCAACA TTCCTATAAT CACTCGAATT ATGATGGTTT CACAATTCTT 
CTTCAAACAT CTCTTGGACG ATACGATGAG GATCCATCTC TGAGAATGGC AGCATTGGGA 
TGTATTCAAC GTTGTGTTTA TGTGGAACCA GAATGCTGGA AAGCTATTAT TCAAAGAGTC 
AAAAGTTCAG AGGAAAAGTC AAGCTCCGCG TCGAAAAGAC AATCGAAATA TGAAATAATC 
ATGAATCATC TTGAACGAAT GTGGACAGAA GTTCGAAAAA CTGATGGAAT TATGGCACTC 
GTCAACTTGA TTAACTGCAA AATTCCACTG ACAGAAGCGG ATTCTATCCG AAAAACGGCA 
ACTAACACAT TAACGGGACT GGCAAGGCAT CCGGAAGTTC GACAGATTCT TGCAAAATTA 
CCGTTGATAG CTCATAATGG ACTACAGAAT TTAATGCGAG AACCAGTGTG TTCTGACAAG 
CGAGATATTC ATGCAGCATT CTGCAAAGAA GCAGTTCAAC TTCTTCAGGT CATTTATGGA 
AGAAAAATTC ATGATCAACA AGGAAAAGAA ATTCAATCGT CGGAAAAGAG TCACCGTCAA 
TGGGTCATCG AAAATACACA AGTGAGCTTC AATCAAGCAG AACTTCTTCA ATTAATTCAT 
GATCACTTGT TGAAAAGCAA GTTAGATTCA GTAGCAGCAA TGCTGAAATC TGAAGCAAAG 
CTTCCTGATC GTCCAGCAAG TCGGTCAATC AATACTCCGA TTCTCAATAA GCCATTGCCC 
AGCAGCGGAA ATAATTTTTC AAAAATAAAC GACACTTATC CAACATTGGC TCCAAGGACG 
CTTGAATCGG AAATTGGTGG AATTTCAGCA AGACGGCCCT CAAACGCAGC TTCACTGTCA 
TCTCCCGCAA TGGCTACACG TTCTCATTCA ACAGATGACG ATGTTTTCGC CACACCAACT 
TTACCTAGAA GATATACAAC CAGTGGTGCT TTTCCAAAGA AGTTGATGAT CAGTCCTGCA 
CGTCAAAAAC TGAGACCTCT GACTCCAGGA GAAAGTTCAA GTGGATATCG ACCAATTAAA 
GATCTAAACA GTATTGTTAC TGATTATTTC AGAAACCAAC ATTCAACATG TAAGAATCCA 
GTGACAACGT GTCCTCCATT TTCATTATTT TACCAACACA AATGTCCCGA ATTGTCGTAT 
CAAACGAATG TTGTGAGAAA TATTTCTCTT CGAACACTCG ATCAAGAACT TCTTAGACCT 
CATGAAAGAG TTTATTCACA GTGGACAAAT GAAAGAACTA TTTTCTCAAG ATTCAGGAAC 
TGGAAAACTA TACATGATCA CGATGAATCG TATACGAAAG CCACATTTTC GGTCGACGAT 
GAACATCTTA TCGTTGGTTT ATTCAATGGA GAAGTTCATT GGATAAACGT GGATACTGGA 
TTGGATGAAG GACACACCAA TTGCCATGGA AGTGCTCTGA CGAATATTGA ACCTTCGAAG 
GATGGTTCAA TGATGCTCAC GTCTTCAGCA TTCTCTCGTC CACTATCCGC TCTTTGGAGA 
CTCGGAGAAG CTTTACAACG AGTTCATACT TATCGTGAAG ATTCTTGTGT TAAATTTGCT 
AACACAACGA TGCAAAGAAT TGTCGGAACG TGTAGAGATA AGGCAACTGT CTATGACACT 
GAAACAAATC ATGTTTTGGA CACGTATCTC TCTGGAATAG ATGGACTACA GTACGAGAAA 
AATTACGCTT CGTTTTCGCC TGATGACAAG CTAATCTTCA ATGATGGACT TCTCTGGGAT 
GTCAGAAAGA AGAATTCGGC GATCCATGTA TTTGATCGTC TCTCCAAAAT AACATTATTC 
GGAACATTCC ATCCTCATGG AACACAGATT GTTATAAATT CTGAAGTTTA TGATATTCGT 
ACATTCCGGA TGCTTCACCA TGTTCCTGAG CTCAATCGTT GTCAAGTTTC ATTTAATTCC 
ACTGGTAATA TCATGTACGC CACTGAAGTT ACAGACGTTC ATTGTCCAGA CTATGATGAA 
AAGATCTTCT CGTCATTCCG AACGTTCGAG ACCCGTGACT ATTCTGCTCT GACAACTTTC 
GAAGGACGAC GACCTGTCAT TGATCTATGC GCATCACATC AAGATCAGAA AATGTGTGTG 
ATTGAGAAAG TGCGTCCACA AATGTCGGAT TACATGATAC AAGCATCTAC ACAACTGAAA 
ATAGTTGAAA TTGGACGATT GAAGGATAAT GAAGATGAGA ATGACGAAGA GGAAGATGAA 
CAGAGAGAGG ATCACGATGA AGACGAAGAT TCGGATGAAT CTGGAGATGG AGATGATGAT 
GAAGAGATTG GAGGAAATAG TTCAGATCGA GAATCCGTGT TTCGAACTTT AGGAAGACTT 
GGCGATGAAA GTGACAGTGG AAGTAGCGTG GATGACAATG ATACACTAGA CGATTTGGAC 
TTTGAAAATG CACAGAATCG GATTATCAGA CGTCAAGCTC AACGCCGTCG TCAACGCCTA 
AACAGTAGTG AAAATGATGC AGAGCTTCCA GGTTCTGATG AAGGAAGTGA TGAAGATGGA 
GATGATGATG AGGATGGAGA AGGGGATCCA GATTTTGATA TGGGAGCGGC TATTGATGAT 
TTGGTAGATG CAGTTGATGA AGAAGTTGAT GAAGATGAAT TGGGAACTGA TGGTGATGAT 
GATGATAGTG GAAGTTGGAG AACAACTAAT TCCATTGACT CAGAAGACAT TAATCTAGAC 
GATCTCGACG AAGAAGAAGC TCGTGTCGTG GAAAATGAGG GCAACAACGA ACGCCCAGCG 
CGTCCAGTAG ATCCAATAGA AGCAGCAGCT GCTGCTCGTC GAGCCATCCT CGGCCGCGGT 
CTCCGAGATC TTCGAATGGG AATTAGAGGA GGTAATCGTC GTCGAAATGG AGGTGGTTTA 
GAGCAAGCTG AAATGTTGAA TGCCCGAGCT GAAGAGCAAC GAGTTTCGTT CATGGAGGCT 
CTTGTTAGAG GAGCTGAGGC TGAACGGAGA GAGGAAGAAG GTGGCGATGA CGGAGAAAGC 
AGTAGCAGCA GTAGTAGTGA TACTGATGAA TATCAATCCG AAGAGGAGGA GATCAACAGT 
GTCAGTACAA CCGCCCTCAA TCCAGCTCTG CGTCGAAGAA ATCGTCGTCC TGACGATGAA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_501725.2
CDS	1..5106
Translation	MTSKEIYPLRSTGTMMMISDQDLKLSTQLMLRIAELLLEFDANHEQSSFDPIPILKRISELLEQATDIFIKNDPDPLDDRHPHRTHPDSALGNILKIIFKNDDFMTKLVVSYILARDNVELNIQGSRLLLACIPGLDSKVVFSEPDDFIPRLYTWAGSEGTNETLQGYAMGLLAAALENTENASKYRNENALLVPFGLRRLHELQGRSLEEQKKIGQTDFSQLHAEQSTSNGTSIPSIKITSVDGSTKENEKTFVQSTDPPPPKKRRTEPCLTSLLRTEITQRVPSFHNLRNLDDSNSKWDILQPFLIGDQQVYPLSLATYQRFILQYLAACGEYQDLLLQTFEGNALEILFDYIDLEKSKDVRLTFDALKYLTSLLVHRKFALEFVNKGGIPALLKVPKTSLASVGVVTCLYYIAYSNDVMEILCQMSDEIVDETVQYVLWCLEHSHESGMASACMFFSQGLFYKAILRRFDQYDGPRKLHNYIATLTLMQNNEDVELTEEQIHTSTQCTRGVCTTFRSYLTAHIFIKVENYKKLYGNNLPTGMRFPELVQGDCPDYKSMKPYEEVCWQCEAIVTEMLRFTGSSFREAENLRKLGMVRMFLAVRVLSRDWENISPSLRTEMCVHALETLCMMFCLPSIQTELITQHSYNHSNYDGFTILLQTSLGRYDEDPSLRMAALGCIQRCVYVEPECWKAIIQRVKSSEEKSSSASKRQSKYEIIMNHLERMWTEVRKTDGIMALVNLINCKIPLTEADSIRKTATNTLTGLARHPEVRQILAKLPLIAHNGLQNLMREPVCSDKRDIHAAFCKEAVQLLQVIYGRKIHDQQGKEIQSSEKSHRQWVIENTQVSFNQAELLQLIHDHLLKSKLDSVAAMLKSEAKLPDRPASRSINTPILNKPLPSSGNNFSKINDTYPTLAPRTLESEIGGISARRPSNAASLSSPAMATRSHSTDDDVFATPTLPRRYTTSGAFPKKLMISPARQKLRPLTPGESSSGYRPIKDLNSIVTDYFRNQHSTCKNPVTTCPPFSLFYQHKCPELSYQTNVVRNISLRTLDQELLRPHERVYSQWTNERTIFSRFRNWKTIHDHDESYTKATFSVDDEHLIVGLFNGEVHWINVDTGLDEGHTNCHGSALTNIEPSKDGSMMLTSSAFSRPLSALWRLGEALQRVHTYREDSCVKFANTTMQRIVGTCRDKATVYDTETNHVLDTYLSGIDGLQYEKNYASFSPDDKLIFNDGLLWDVRKKNSAIHVFDRLSKITLFGTFHPHGTQIVINSEVYDIRTFRMLHHVPELNRCQVSFNSTGNIMYATEVTDVHCPDYDEKIFSSFRTFETRDYSALTTFEGRRPVIDLCASHQDQKMCVIEKVRPQMSDYMIQASTQLKIVEIGRLKDNEDENDEEEDEQREDHDEDEDSDESGDGDDDEEIGGNSSDRESVFRTLGRLGDESDSGSSVDDNDTLDDLDFENAQNRIIRRQAQRRRQRLNSSENDAELPGSDEGSDEDGDDDEDGEGDPDFDMGAAIDDLVDAVDEEVDEDELGTDGDDDDSGSWRTTNSIDSEDINLDDLDEEEARVVENEGNNERPARPVDPIEAAAAARRAILGRGLRDLRMGIRGGNRRRNGGGLEQAEMLNARAEEQRVSFMEALVRGAEAERREEEGGDDGESSSSSSSDTDEYQSEEEEINSVSTTALNPALRRRNRRPDDEA

Target ORF information:

RefSeq Version	NM_069324.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DDB1-and CUL4-associated factor homolog 1 (dcaf-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069324.5

ORF Insert Sequence:

ATGACGAGCA AGGAGATTTA TCCGCTCCGA TCGACAGGAA CAATGATGAT GATATCCGAT 
CAAGATTTGA AGCTGAGCAC GCAATTAATG CTTCGAATAG CTGAATTACT ATTGGAGTTT 
GATGCAAATC ATGAACAATC GTCGTTTGAT CCGATACCGA TACTTAAAAG AATTTCTGAA 
TTGTTGGAAC AAGCTACAGA CATTTTCATT AAAAACGACC CGGATCCTTT GGATGATCGT 
CATCCTCACA GGACTCATCC AGATTCTGCA CTCGGCAACA TTTTGAAAAT TATATTCAAA 
AACGATGATT TCATGACAAA ACTTGTTGTG TCGTATATTC TTGCCAGAGA CAACGTAGAA 
TTGAATATTC AAGGAAGTCG ACTTCTGTTA GCCTGTATTC CTGGTCTCGA TTCAAAAGTA 
GTTTTCTCAG AACCAGACGA TTTCATTCCT CGCCTATACA CATGGGCAGG ATCAGAAGGA 
ACAAATGAGA CTTTACAAGG TTATGCAATG GGACTTCTAG CTGCTGCATT GGAAAATACT 
GAAAATGCGA GTAAATATCG AAATGAAAAT GCTCTATTAG TCCCATTTGG ACTTCGGCGA 
CTCCATGAAT TACAGGGTCG CTCTTTGGAA GAACAGAAGA AAATTGGACA GACTGATTTC 
TCCCAACTGC ATGCTGAACA AAGTACAAGT AACGGAACTT CAATTCCTTC AATCAAAATC 
ACTTCTGTTG ATGGTTCTAC AAAAGAGAAT GAGAAAACCT TTGTACAATC AACAGATCCA 
CCACCACCGA AAAAGCGAAG AACTGAGCCT TGCCTGACAT CTCTTCTTCG AACGGAAATT 
ACTCAGCGAG TTCCATCATT TCATAATCTT CGAAATTTGG ATGATTCAAA CAGTAAATGG 
GATATTCTTC AACCATTTCT AATTGGAGAT CAACAAGTAT ATCCACTTAG TTTAGCTACA 
TATCAACGGT TTATTCTACA ATATTTGGCT GCATGTGGAG AATATCAGGA TCTCCTCCTT 
CAAACTTTTG AAGGAAATGC ATTGGAAATC TTGTTCGACT ACATTGATCT CGAAAAATCA 
AAAGATGTCC GTTTAACCTT TGATGCTTTA AAATATCTCA CTTCTCTATT GGTACATCGT 
AAATTCGCCT TGGAATTCGT CAACAAAGGA GGAATACCGG CTCTTTTGAA AGTTCCAAAA 
ACATCATTGG CTTCAGTTGG AGTTGTTACA TGTCTGTATT ACATTGCATA CAGTAATGAT 
GTCATGGAGA TTTTATGTCA AATGAGCGAT GAGATTGTGG ATGAGACTGT ACAGTATGTT 
CTGTGGTGCT TGGAACACAG TCATGAAAGT GGAATGGCCA GTGCATGTAT GTTCTTTTCT 
CAAGGATTGT TCTACAAGGC TATTTTAAGA AGATTTGATC AATATGATGG TCCACGGAAG 
CTGCATAATT ATATTGCAAC CCTGACACTA ATGCAAAACA ATGAAGATGT CGAACTCACC 
GAAGAACAAA TACATACAAG TACACAGTGT ACAAGAGGAG TTTGTACCAC TTTCAGAAGT 
TACCTCACCG CTCACATTTT CATCAAAGTG GAAAACTATA AAAAGCTATA CGGAAATAAC 
TTACCAACTG GAATGCGATT TCCAGAACTT GTACAAGGAG ATTGTCCAGA TTATAAAAGC 
ATGAAGCCGT ATGAAGAAGT TTGTTGGCAG TGCGAAGCCA TTGTTACGGA AATGCTTCGA 
TTCACCGGAA GTTCATTCAG AGAGGCTGAA AATTTAAGAA AACTAGGAAT GGTTCGAATG 
TTTTTGGCGG TTAGAGTGTT ATCAAGAGAT TGGGAAAACA TTTCTCCAAG TCTCCGCACC 
GAAATGTGTG TACACGCATT GGAAACATTG TGCATGATGT TTTGCCTTCC ATCAATTCAA 
ACTGAACTTA TCACTCAACA TTCCTATAAT CACTCGAATT ATGATGGTTT CACAATTCTT 
CTTCAAACAT CTCTTGGACG ATACGATGAG GATCCATCTC TGAGAATGGC AGCATTGGGA 
TGTATTCAAC GTTGTGTTTA TGTGGAACCA GAATGCTGGA AAGCTATTAT TCAAAGAGTC 
AAAAGTTCAG AGGAAAAGTC AAGCTCCGCG TCGAAAAGAC AATCGAAATA TGAAATAATC 
ATGAATCATC TTGAACGAAT GTGGACAGAA GTTCGAAAAA CTGATGGAAT TATGGCACTC 
GTCAACTTGA TTAACTGCAA AATTCCACTG ACAGAAGCGG ATTCTATCCG AAAAACGGCA 
ACTAACACAT TAACGGGACT GGCAAGGCAT CCGGAAGTTC GACAGATTCT TGCAAAATTA 
CCGTTGATAG CTCATAATGG ACTACAGAAT TTAATGCGAG AACCAGTGTG TTCTGACAAG 
CGAGATATTC ATGCAGCATT CTGCAAAGAA GCAGTTCAAC TTCTTCAGGT CATTTATGGA 
AGAAAAATTC ATGATCAACA AGGAAAAGAA ATTCAATCGT CGGAAAAGAG TCACCGTCAA 
TGGGTCATCG AAAATACACA AGTGAGCTTC AATCAAGCAG AACTTCTTCA ATTAATTCAT 
GATCACTTGT TGAAAAGCAA GTTAGATTCA GTAGCAGCAA TGCTGAAATC TGAAGCAAAG 
CTTCCTGATC GTCCAGCAAG TCGGTCAATC AATACTCCGA TTCTCAATAA GCCATTGCCC 
AGCAGCGGAA ATAATTTTTC AAAAATAAAC GACACTTATC CAACATTGGC TCCAAGGACG 
CTTGAATCGG AAATTGGTGG AATTTCAGCA AGACGGCCCT CAAACGCAGC TTCACTGTCA 
TCTCCCGCAA TGGCTACACG TTCTCATTCA ACAGATGACG ATGTTTTCGC CACACCAACT 
TTACCTAGAA GATATACAAC CAGTGGTGCT TTTCCAAAGA AGTTGATGAT CAGTCCTGCA 
CGTCAAAAAC TGAGACCTCT GACTCCAGGA GAAAGTTCAA GTGGATATCG ACCAATTAAA 
GATCTAAACA GTATTGTTAC TGATTATTTC AGAAACCAAC ATTCAACATG TAAGAATCCA 
GTGACAACGT GTCCTCCATT TTCATTATTT TACCAACACA AATGTCCCGA ATTGTCGTAT 
CAAACGAATG TTGTGAGAAA TATTTCTCTT CGAACACTCG ATCAAGAACT TCTTAGACCT 
CATGAAAGAG TTTATTCACA GTGGACAAAT GAAAGAACTA TTTTCTCAAG ATTCAGGAAC 
TGGAAAACTA TACATGATCA CGATGAATCG TATACGAAAG CCACATTTTC GGTCGACGAT 
GAACATCTTA TCGTTGGTTT ATTCAATGGA GAAGTTCATT GGATAAACGT GGATACTGGA 
TTGGATGAAG GACACACCAA TTGCCATGGA AGTGCTCTGA CGAATATTGA ACCTTCGAAG 
GATGGTTCAA TGATGCTCAC GTCTTCAGCA TTCTCTCGTC CACTATCCGC TCTTTGGAGA 
CTCGGAGAAG CTTTACAACG AGTTCATACT TATCGTGAAG ATTCTTGTGT TAAATTTGCT 
AACACAACGA TGCAAAGAAT TGTCGGAACG TGTAGAGATA AGGCAACTGT CTATGACACT 
GAAACAAATC ATGTTTTGGA CACGTATCTC TCTGGAATAG ATGGACTACA GTACGAGAAA 
AATTACGCTT CGTTTTCGCC TGATGACAAG CTAATCTTCA ATGATGGACT TCTCTGGGAT 
GTCAGAAAGA AGAATTCGGC GATCCATGTA TTTGATCGTC TCTCCAAAAT AACATTATTC 
GGAACATTCC ATCCTCATGG AACACAGATT GTTATAAATT CTGAAGTTTA TGATATTCGT 
ACATTCCGGA TGCTTCACCA TGTTCCTGAG CTCAATCGTT GTCAAGTTTC ATTTAATTCC 
ACTGGTAATA TCATGTACGC CACTGAAGTT ACAGACGTTC ATTGTCCAGA CTATGATGAA 
AAGATCTTCT CGTCATTCCG AACGTTCGAG ACCCGTGACT ATTCTGCTCT GACAACTTTC 
GAAGGACGAC GACCTGTCAT TGATCTATGC GCATCACATC AAGATCAGAA AATGTGTGTG 
ATTGAGAAAG TGCGTCCACA AATGTCGGAT TACATGATAC AAGCATCTAC ACAACTGAAA 
ATAGTTGAAA TTGGACGATT GAAGGATAAT GAAGATGAGA ATGACGAAGA GGAAGATGAA 
CAGAGAGAGG ATCACGATGA AGACGAAGAT TCGGATGAAT CTGGAGATGG AGATGATGAT 
GAAGAGATTG GAGGAAATAG TTCAGATCGA GAATCCGTGT TTCGAACTTT AGGAAGACTT 
GGCGATGAAA GTGACAGTGG AAGTAGCGTG GATGACAATG ATACACTAGA CGATTTGGAC 
TTTGAAAATG CACAGAATCG GATTATCAGA CGTCAAGCTC AACGCCGTCG TCAACGCCTA 
AACAGTAGTG AAAATGATGC AGAGCTTCCA GGTTCTGATG AAGGAAGTGA TGAAGATGGA 
GATGATGATG AGGATGGAGA AGGGGATCCA GATTTTGATA TGGGAGCGGC TATTGATGAT 
TTGGTAGATG CAGTTGATGA AGAAGTTGAT GAAGATGAAT TGGGAACTGA TGGTGATGAT 
GATGATAGTG GAAGTTGGAG AACAACTAAT TCCATTGACT CAGAAGACAT TAATCTAGAC 
GATCTCGACG AAGAAGAAGC TCGTGTCGTG GAAAATGAGG GCAACAACGA ACGCCCAGCG 
CGTCCAGTAG ATCCAATAGA AGCAGCAGCT GCTGCTCGTC GAGCCATCCT CGGCCGCGGT 
CTCCGAGATC TTCGAATGGG AATTAGAGGA GGTAATCGTC GTCGAAATGG AGGTGGTTTA 
GAGCAAGCTG AAATGTTGAA TGCCCGAGCT GAAGAGCAAC GAGTTTCGTT CATGGAGGCT 
CTTGTTAGAG GAGCTGAGGC TGAACGGAGA GAGGAAGAAG GTGGCGATGA CGGAGAAAGC 
AGTAGCAGCA GTAGTAGTGA TACTGATGAA TATCAATCCG AAGAGGAGGA GATCAACAGT 
GTCAGTACAA CCGCCCTCAA TCCAGCTCTG CGTCGAAGAA ATCGTCGTCC TGACGATGAA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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dcaf-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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