sbp-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	sbp-1
Entrez Gene ID	176574
Full Name	Sterol regulatory element Binding Protein
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_067071.6	NP_499472.1	BHLH domain-containing protein
NM_067071.5	NP_499472.1	Sterol regulatory element Binding Protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the ability of Pu-erh tea in promoting inhibition of food uptake and the biosynthesis of fat via SBP-1 and SCD, thereby reducing fat storage.
Title: Pu-erh tea down-regulates sterol regulatory element-binding protein and stearyol-CoA desaturase to reduce fat storage in Caenorhaditis elegans.

SBP-1/SREBP-1 is part of a conserved feedback loop responding to phosphatidylcholine levels to regulate expression of one-carbon cycle biogenesis genes and ensure adequate S-adenosylmethionine levels for phosphatidylcholine production.
Title: A conserved SREBP-1/phosphatidylcholine feedback circuit regulates lipogenesis in metazoans.

Essential role of sbp-1 activation in oxygen deprivation induced lipid accumulation and increase in body width/length ratio in Caenorhabditis elegans.
Title: Essential role of SBP-1 activation in oxygen deprivation induced lipid accumulation and increase in body width/length ratio in Caenorhabditis elegans.

both SBP-1 and MDT-15 control transcription of genes governing desaturation of stearic acid to oleic acid
Title: An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis.

elo-5 and elo-6 may be transcriptional targets of LPD-1
Title: Monomethyl branched-chain fatty acids play an essential role in Caenorhabditis elegans development.

The following sbp-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sbp-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340456	NM_067071.6 Latest version!	Caenorhabditis elegans BHLH domain-containing protein (sbp-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa340456	NM_067071.5	Caenorhabditis elegans Sterol regulatory element Binding Protein (sbp-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sbp-1 ( NM_067071.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499472.1 Caenorhabditis elegans BHLH domain-containing protein (sbp-1), mRNA.

Price & Availability↑

CloneID	OCa340456
Clone ID Related Accession (Same CDS sequence)	NM_067071.6 , NM_067071.5
Accession Version	NM_067071.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	BHLH domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_067071.5.

ATGAACGAAG AATTCGAGGG AGACGTCCCT ATGTCGGATC CGTTTCTCTC ATTGGTCACA 
AAATTGGATG ATATTGCGCC ATTTCCAAAT AACGACCCGC TCGATTTTGA CATGGAGCAC 
AACTGGCAAG AGCCCGGACC ATCACAACAA CCGGATCCAT CAATTCCCGG AAATCAACAC 
AGTCCGCCAC AGGAATATTA TGATATTGAT GGTCAACGAG ACGTAAGCAC CTTACACTCC 
CTGCTCAACC ACAACAACGA CGACTTCTTC TCAATGCGAT TTTCCCCGCC AAACTTTGAT 
CTCGGCGGAG GCCGTGGACC TTCTCTAGCC GCCACCCAAC AATTATCTGG AGAAGGTCCT 
GCAAGTATGC TTAACCCCTT ACAAACATCT CCACCAAGTG GAGGTTACCC CCCGGCAGAT 
GCCTACAGAC CTCTATCACT TGCTCAACAA CTCGCCGCGC CAGCGATGAC TCCACATCAG 
GCAGCGTCGC TTTTTGTTAA TACTAATGGA ATTGATCAAA AGAATTTCAC TCATGCAATG 
CTATCTCCAC CACACCATAC CTCAATGACT CCTCAACCAT ATACAGAAGC AATGGAACAT 
ATCAACGGGT ACATGTCTCC ATACGACCAA GCTCAAGGCC CATCAGGACC ATCATATTAC 
TCACAACACC ATCAATCTCC ACCACCTCAT CACCACCATC ACCACCCGAT GCCAAAAATC 
CATGAGAACC CTGAACAAGT GGCATCTCCA TCGATTGAAG ATGCTCCAGA GACGAAACCA 
ACTCATTTGG TTGAACCACA AAGTCCAAAA AGCCCGCAGA ATATGAAAGA GGAGCTTCTT 
CGGTTACTAG TTAACATGTC TCCGAGTGAA GTTGAACGGT TAAAGAATAA AAAATCAGGA 
GCATGTTCAG CGACGAATGG GCCATCGAGG AGTAAGGAGA AGGCGGCGAA GATTGTGATT 
CAGGAGACAG CGGAAGGGGA TGAAGATGAG GATGATGAGG ATAGTGATTC CGGGGAGACT 
ATGTCTCAGG GAACTACTAT TATTGTTCGA AGACCAAAAA CCGAGCGTCG TACGGCACAC 
AATCTCATCG AAAAGAAGTA TAGATGCTCA ATAAATGATC GAATTCAACA GCTGAAAGTA 
CTTTTGTGTG GGGATGAAGC TAAGCTTTCA AAATCGGCAA CACTACGACG GGCTATTGAA 
CATATCGAGG AGGTTGAACA CGAGAATCAG GTGTTGAAGC ATCATGTTGA ACAAATGAGA 
AAGACACTGC AGAATAATCG ATTACCGTAC CCGGAACCAA TTCAATACAC TGAATACTCT 
GCCCGATCAC CCGTCGAATC ATCTCCTTCT CCACCTAGAA ATGAGAGAAA ACGATCACGA 
ATGAGCACAA CGACTCCTAT GAAGAATGGA ACTAGAGATG GATCTTCGAA AGTTACCCTT 
TTTGCGATGC TCCTAGCAGT TCTGATTTTT AATCCGATTG GATTGCTCGC TGGAAGTGCG 
ATATTCTCAA AAGCCGCTGC AGAAGCTCCG ATTGCCTCCC CGTTCGAGCA TGGAAGAGTG 
ATTGATGACC CGGATGGAAC TAGCACTCGG ACGCTTTTCT GGGAAGGGAG TATCATCAAT 
ATGAGCTATG TCTGGGTGTT CAACATCTTA ATGATCATAT ATGTGGTTGT CAAACTGCTG 
ATCCATGGTG ACCCTGTTCA AGACTTCATG TCCGTTTCAT GGCAGACTTT TGTGACGACT 
CGAGAGAAGG CGAGAGCCGA GTTGAACTCT GGAAATTTGA AAGATGCTCA GAGAAAGTTC 
TGCGAGTGTC TTGCAACGTT GGATCGATCG CTTCCATCAC CGGGGGTTGA TTCGGTGTTT 
TCGGTTGGCT GGGAATGCGT TCGACATCTT TTGAATTGGT TGTGGATCGG GAGATACATC 
GCAAGAAGGC GCAGGTCCAC CACGAAGCCT GTCTCAGTCG TTTGTAGGAG TCATGCGCAG 
ACTGCAGTTC TCTATCATGA AATTCATCAG CTCCATCTAA TGGGTATCAC TGGAAACTTC 
GAAGACACCT ATGAACCATC CGCCCTAACG GGCCTCTTCA TGTCCCTCTG TGCAGTAAAC 
CTTGCTGAAG CTGCCGGAGC ATCAAACGAC GGACTTCCAC GCGCCGTCAT GGCTCAGATC 
TACATTTCTG CATCCATCCA ATGCCGTTTG GCTCTTCCGA ACCTACTCGC ACCATTCTTC 
TCGGGATACT TTTTACGAAG AGCTCGAAGG CACGTGCGTC GAGCTCCGGA GCACTCGGTG 
TCCCATTTGT TATGGATCTT CCATCCAGCG ACAAGAAAGT TCATGTCAGA TGCGAAAAGG 
TTGGAGCATG TGTTGAGCTC GAAGCAGAAG CAGTTGAGAT TTGGGTCTTT TGTGGAAGAT 
GAGCAATTAT CCCCACTTGC TCGAATCCGA ACAACGCTGA AAGTGTACCT ACTCTCCAAA 
CTTGTACAGG AACTTGTCGG TGGTGACGAG ATCTTTACAA AAAATGTGGA ACGCATCCTA 
AATGACAATG ACCGTCTCGA TGATGAAGTA GACGTGGTTG ATGTTTCAAG ACTTTTGGTG 
ACAATTTCAA CGCAGTGCGC TGCCATTTTG ACTAATGAGA AGGATGAGTC AGCGAAATTC 
GGAACCTGGA TCTCTCGAAA CGGAGATGCT TGTTGCACAT GGTGGACGCA CGTTCTGACA 
TGTGGAATCT ATTGGAGGAG TAACAAGAAT GAGCTGGCAC GGCAACACTA TTCACTGATC 
AGGAACTGTC CGCCGAAGAT TTTGACAGAC AATCTGGGTT TGGCGGTTGG CCACGCGTTG 
TGTGCTCGCA AGATTTGCAT AGATGACCGA GATTCCCCGA AAGTCAGTCA ATACGTGTGC 
ATTCACACAA AGAAGTCGCT CGAATCCCTC CGACTATTCT CCACATCATC GCGAGCATCA 
GGTGTGGTGT CTGGAATTCA GGAAGGTACA CGCCGAATGG CCTACGAATG GATTATGAAC 
TCGCTGCTCG ACGCGTGGCG TTCCAATCTA TTCGCATCGA AACCCTACTG GACACAAAGC 
TTCAAGGGAC AATCCACGTT TAGTACGCTT TATCAAGAGG CGTATAATCA TTATGCGATT 
ATTAATGGGA CAAGGGGAGA TTGTTGGAGA CTATTTGTCT ACGAGCTCAC GTGCCGAATG 
CTCAACGGAG CCAACCCACA AGCCACGTGG TCAGGCGTCC GACGCGTTCG ATCTACAAAA 
ATGGACGCGG TCCGAGGAAG AGTGAGCATG CGACGCTCGG CTCAACCGGA CGCATTTCAT 
CTTCATACAC TGGTTAAACT ACATACTTCT ATGGATCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499472.1
CDS	2..3343
Translation	MNEEFEGDVPMSDPFLSLVTKLDDIAPFPNNDPLDFDMEHNWQEPGPSQQPDPSIPGNQHSPPQEYYDIDGQRDVSTLHSLLNHNNDDFFSMRFSPPNFDLGGGRGPSLAATQQLSGEGPASMLNPLQTSPPSGGYPPADAYRPLSLAQQLAAPAMTPHQAASLFVNTNGIDQKNFTHAMLSPPHHTSMTPQPYTEAMEHINGYMSPYDQAQGPSGPSYYSQHHQSPPPHHHHHHPMPKIHENPEQVASPSIEDAPETKPTHLVEPQSPKSPQNMKEELLRLLVNMSPSEVERLKNKKSGACSATNGPSRSKEKAAKIVIQETAEGDEDEDDEDSDSGETMSQGTTIIVRRPKTERRTAHNLIEKKYRCSINDRIQQLKVLLCGDEAKLSKSATLRRAIEHIEEVEHENQVLKHHVEQMRKTLQNNRLPYPEPIQYTEYSARSPVESSPSPPRNERKRSRMSTTTPMKNGTRDGSSKVTLFAMLLAVLIFNPIGLLAGSAIFSKAAAEAPIASPFEHGRVIDDPDGTSTRTLFWEGSIINMSYVWVFNILMIIYVVVKLLIHGDPVQDFMSVSWQTFVTTREKARAELNSGNLKDAQRKFCECLATLDRSLPSPGVDSVFSVGWECVRHLLNWLWIGRYIARRRRSTTKPVSVVCRSHAQTAVLYHEIHQLHLMGITGNFEDTYEPSALTGLFMSLCAVNLAEAAGASNDGLPRAVMAQIYISASIQCRLALPNLLAPFFSGYFLRRARRHVRRAPEHSVSHLLWIFHPATRKFMSDAKRLEHVLSSKQKQLRFGSFVEDEQLSPLARIRTTLKVYLLSKLVQELVGGDEIFTKNVERILNDNDRLDDEVDVVDVSRLLVTISTQCAAILTNEKDESAKFGTWISRNGDACCTWWTHVLTCGIYWRSNKNELARQHYSLIRNCPPKILTDNLGLAVGHALCARKICIDDRDSPKVSQYVCIHTKKSLESLRLFSTSSRASGVVSGIQEGTRRMAYEWIMNSLLDAWRSNLFASKPYWTQSFKGQSTFSTLYQEAYNHYAIINGTRGDCWRLFVYELTCRMLNGANPQATWSGVRRVRSTKMDAVRGRVSMRRSAQPDAFHLHTLVKLHTSMDL

Target ORF information:

RefSeq Version	NM_067071.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans BHLH domain-containing protein (sbp-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_067071.6

ORF Insert Sequence:

ATGAACGAAG AATTCGAGGG AGACGTCCCT ATGTCGGATC CGTTTCTCTC ATTGGTCACA 
AAATTGGATG ATATTGCGCC ATTTCCAAAT AACGACCCGC TCGATTTTGA CATGGAGCAC 
AACTGGCAAG AGCCCGGACC ATCACAACAA CCGGATCCAT CAATTCCCGG AAATCAACAC 
AGTCCGCCAC AGGAATATTA TGATATTGAT GGTCAACGAG ACGTAAGCAC CTTACACTCC 
CTGCTCAACC ACAACAACGA CGACTTCTTC TCAATGCGAT TTTCCCCGCC AAACTTTGAT 
CTCGGCGGAG GCCGTGGACC TTCTCTAGCC GCCACCCAAC AATTATCTGG AGAAGGTCCT 
GCAAGTATGC TTAACCCCTT ACAAACATCT CCACCAAGTG GAGGTTACCC CCCGGCAGAT 
GCCTACAGAC CTCTATCACT TGCTCAACAA CTCGCCGCGC CAGCGATGAC TCCACATCAG 
GCAGCGTCGC TTTTTGTTAA TACTAATGGA ATTGATCAAA AGAATTTCAC TCATGCAATG 
CTATCTCCAC CACACCATAC CTCAATGACT CCTCAACCAT ATACAGAAGC AATGGAACAT 
ATCAACGGGT ACATGTCTCC ATACGACCAA GCTCAAGGCC CATCAGGACC ATCATATTAC 
TCACAACACC ATCAATCTCC ACCACCTCAT CACCACCATC ACCACCCGAT GCCAAAAATC 
CATGAGAACC CTGAACAAGT GGCATCTCCA TCGATTGAAG ATGCTCCAGA GACGAAACCA 
ACTCATTTGG TTGAACCACA AAGTCCAAAA AGCCCGCAGA ATATGAAAGA GGAGCTTCTT 
CGGTTACTAG TTAACATGTC TCCGAGTGAA GTTGAACGGT TAAAGAATAA AAAATCAGGA 
GCATGTTCAG CGACGAATGG GCCATCGAGG AGTAAGGAGA AGGCGGCGAA GATTGTGATT 
CAGGAGACAG CGGAAGGGGA TGAAGATGAG GATGATGAGG ATAGTGATTC CGGGGAGACT 
ATGTCTCAGG GAACTACTAT TATTGTTCGA AGACCAAAAA CCGAGCGTCG TACGGCACAC 
AATCTCATCG AAAAGAAGTA TAGATGCTCA ATAAATGATC GAATTCAACA GCTGAAAGTA 
CTTTTGTGTG GGGATGAAGC TAAGCTTTCA AAATCGGCAA CACTACGACG GGCTATTGAA 
CATATCGAGG AGGTTGAACA CGAGAATCAG GTGTTGAAGC ATCATGTTGA ACAAATGAGA 
AAGACACTGC AGAATAATCG ATTACCGTAC CCGGAACCAA TTCAATACAC TGAATACTCT 
GCCCGATCAC CCGTCGAATC ATCTCCTTCT CCACCTAGAA ATGAGAGAAA ACGATCACGA 
ATGAGCACAA CGACTCCTAT GAAGAATGGA ACTAGAGATG GATCTTCGAA AGTTACCCTT 
TTTGCGATGC TCCTAGCAGT TCTGATTTTT AATCCGATTG GATTGCTCGC TGGAAGTGCG 
ATATTCTCAA AAGCCGCTGC AGAAGCTCCG ATTGCCTCCC CGTTCGAGCA TGGAAGAGTG 
ATTGATGACC CGGATGGAAC TAGCACTCGG ACGCTTTTCT GGGAAGGGAG TATCATCAAT 
ATGAGCTATG TCTGGGTGTT CAACATCTTA ATGATCATAT ATGTGGTTGT CAAACTGCTG 
ATCCATGGTG ACCCTGTTCA AGACTTCATG TCCGTTTCAT GGCAGACTTT TGTGACGACT 
CGAGAGAAGG CGAGAGCCGA GTTGAACTCT GGAAATTTGA AAGATGCTCA GAGAAAGTTC 
TGCGAGTGTC TTGCAACGTT GGATCGATCG CTTCCATCAC CGGGGGTTGA TTCGGTGTTT 
TCGGTTGGCT GGGAATGCGT TCGACATCTT TTGAATTGGT TGTGGATCGG GAGATACATC 
GCAAGAAGGC GCAGGTCCAC CACGAAGCCT GTCTCAGTCG TTTGTAGGAG TCATGCGCAG 
ACTGCAGTTC TCTATCATGA AATTCATCAG CTCCATCTAA TGGGTATCAC TGGAAACTTC 
GAAGACACCT ATGAACCATC CGCCCTAACG GGCCTCTTCA TGTCCCTCTG TGCAGTAAAC 
CTTGCTGAAG CTGCCGGAGC ATCAAACGAC GGACTTCCAC GCGCCGTCAT GGCTCAGATC 
TACATTTCTG CATCCATCCA ATGCCGTTTG GCTCTTCCGA ACCTACTCGC ACCATTCTTC 
TCGGGATACT TTTTACGAAG AGCTCGAAGG CACGTGCGTC GAGCTCCGGA GCACTCGGTG 
TCCCATTTGT TATGGATCTT CCATCCAGCG ACAAGAAAGT TCATGTCAGA TGCGAAAAGG 
TTGGAGCATG TGTTGAGCTC GAAGCAGAAG CAGTTGAGAT TTGGGTCTTT TGTGGAAGAT 
GAGCAATTAT CCCCACTTGC TCGAATCCGA ACAACGCTGA AAGTGTACCT ACTCTCCAAA 
CTTGTACAGG AACTTGTCGG TGGTGACGAG ATCTTTACAA AAAATGTGGA ACGCATCCTA 
AATGACAATG ACCGTCTCGA TGATGAAGTA GACGTGGTTG ATGTTTCAAG ACTTTTGGTG 
ACAATTTCAA CGCAGTGCGC TGCCATTTTG ACTAATGAGA AGGATGAGTC AGCGAAATTC 
GGAACCTGGA TCTCTCGAAA CGGAGATGCT TGTTGCACAT GGTGGACGCA CGTTCTGACA 
TGTGGAATCT ATTGGAGGAG TAACAAGAAT GAGCTGGCAC GGCAACACTA TTCACTGATC 
AGGAACTGTC CGCCGAAGAT TTTGACAGAC AATCTGGGTT TGGCGGTTGG CCACGCGTTG 
TGTGCTCGCA AGATTTGCAT AGATGACCGA GATTCCCCGA AAGTCAGTCA ATACGTGTGC 
ATTCACACAA AGAAGTCGCT CGAATCCCTC CGACTATTCT CCACATCATC GCGAGCATCA 
GGTGTGGTGT CTGGAATTCA GGAAGGTACA CGCCGAATGG CCTACGAATG GATTATGAAC 
TCGCTGCTCG ACGCGTGGCG TTCCAATCTA TTCGCATCGA AACCCTACTG GACACAAAGC 
TTCAAGGGAC AATCCACGTT TAGTACGCTT TATCAAGAGG CGTATAATCA TTATGCGATT 
ATTAATGGGA CAAGGGGAGA TTGTTGGAGA CTATTTGTCT ACGAGCTCAC GTGCCGAATG 
CTCAACGGAG CCAACCCACA AGCCACGTGG TCAGGCGTCC GACGCGTTCG ATCTACAAAA 
ATGGACGCGG TCCGAGGAAG AGTGAGCATG CGACGCTCGG CTCAACCGGA CGCATTTCAT 
CTTCATACAC TGGTTAAACT ACATACTTCT ATGGATCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sbp-1 ( NM_067071.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499472.1 Caenorhabditis elegans Sterol regulatory element Binding Protein (sbp-1), partial mRNA.

Price & Availability↑

CloneID	OCa340456
Clone ID Related Accession (Same CDS sequence)	NM_067071.6 , NM_067071.5
Accession Version	NM_067071.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Sterol regulatory element Binding Protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_067071.4. COMPLETENESS: incomplete on both ends.

ATGAACGAAG AATTCGAGGG AGACGTCCCT ATGTCGGATC CGTTTCTCTC ATTGGTCACA 
AAATTGGATG ATATTGCGCC ATTTCCAAAT AACGACCCGC TCGATTTTGA CATGGAGCAC 
AACTGGCAAG AGCCCGGACC ATCACAACAA CCGGATCCAT CAATTCCCGG AAATCAACAC 
AGTCCGCCAC AGGAATATTA TGATATTGAT GGTCAACGAG ACGTAAGCAC CTTACACTCC 
CTGCTCAACC ACAACAACGA CGACTTCTTC TCAATGCGAT TTTCCCCGCC AAACTTTGAT 
CTCGGCGGAG GCCGTGGACC TTCTCTAGCC GCCACCCAAC AATTATCTGG AGAAGGTCCT 
GCAAGTATGC TTAACCCCTT ACAAACATCT CCACCAAGTG GAGGTTACCC CCCGGCAGAT 
GCCTACAGAC CTCTATCACT TGCTCAACAA CTCGCCGCGC CAGCGATGAC TCCACATCAG 
GCAGCGTCGC TTTTTGTTAA TACTAATGGA ATTGATCAAA AGAATTTCAC TCATGCAATG 
CTATCTCCAC CACACCATAC CTCAATGACT CCTCAACCAT ATACAGAAGC AATGGAACAT 
ATCAACGGGT ACATGTCTCC ATACGACCAA GCTCAAGGCC CATCAGGACC ATCATATTAC 
TCACAACACC ATCAATCTCC ACCACCTCAT CACCACCATC ACCACCCGAT GCCAAAAATC 
CATGAGAACC CTGAACAAGT GGCATCTCCA TCGATTGAAG ATGCTCCAGA GACGAAACCA 
ACTCATTTGG TTGAACCACA AAGTCCAAAA AGCCCGCAGA ATATGAAAGA GGAGCTTCTT 
CGGTTACTAG TTAACATGTC TCCGAGTGAA GTTGAACGGT TAAAGAATAA AAAATCAGGA 
GCATGTTCAG CGACGAATGG GCCATCGAGG AGTAAGGAGA AGGCGGCGAA GATTGTGATT 
CAGGAGACAG CGGAAGGGGA TGAAGATGAG GATGATGAGG ATAGTGATTC CGGGGAGACT 
ATGTCTCAGG GAACTACTAT TATTGTTCGA AGACCAAAAA CCGAGCGTCG TACGGCACAC 
AATCTCATCG AAAAGAAGTA TAGATGCTCA ATAAATGATC GAATTCAACA GCTGAAAGTA 
CTTTTGTGTG GGGATGAAGC TAAGCTTTCA AAATCGGCAA CACTACGACG GGCTATTGAA 
CATATCGAGG AGGTTGAACA CGAGAATCAG GTGTTGAAGC ATCATGTTGA ACAAATGAGA 
AAGACACTGC AGAATAATCG ATTACCGTAC CCGGAACCAA TTCAATACAC TGAATACTCT 
GCCCGATCAC CCGTCGAATC ATCTCCTTCT CCACCTAGAA ATGAGAGAAA ACGATCACGA 
ATGAGCACAA CGACTCCTAT GAAGAATGGA ACTAGAGATG GATCTTCGAA AGTTACCCTT 
TTTGCGATGC TCCTAGCAGT TCTGATTTTT AATCCGATTG GATTGCTCGC TGGAAGTGCG 
ATATTCTCAA AAGCCGCTGC AGAAGCTCCG ATTGCCTCCC CGTTCGAGCA TGGAAGAGTG 
ATTGATGACC CGGATGGAAC TAGCACTCGG ACGCTTTTCT GGGAAGGGAG TATCATCAAT 
ATGAGCTATG TCTGGGTGTT CAACATCTTA ATGATCATAT ATGTGGTTGT CAAACTGCTG 
ATCCATGGTG ACCCTGTTCA AGACTTCATG TCCGTTTCAT GGCAGACTTT TGTGACGACT 
CGAGAGAAGG CGAGAGCCGA GTTGAACTCT GGAAATTTGA AAGATGCTCA GAGAAAGTTC 
TGCGAGTGTC TTGCAACGTT GGATCGATCG CTTCCATCAC CGGGGGTTGA TTCGGTGTTT 
TCGGTTGGCT GGGAATGCGT TCGACATCTT TTGAATTGGT TGTGGATCGG GAGATACATC 
GCAAGAAGGC GCAGGTCCAC CACGAAGCCT GTCTCAGTCG TTTGTAGGAG TCATGCGCAG 
ACTGCAGTTC TCTATCATGA AATTCATCAG CTCCATCTAA TGGGTATCAC TGGAAACTTC 
GAAGACACCT ATGAACCATC CGCCCTAACG GGCCTCTTCA TGTCCCTCTG TGCAGTAAAC 
CTTGCTGAAG CTGCCGGAGC ATCAAACGAC GGACTTCCAC GCGCCGTCAT GGCTCAGATC 
TACATTTCTG CATCCATCCA ATGCCGTTTG GCTCTTCCGA ACCTACTCGC ACCATTCTTC 
TCGGGATACT TTTTACGAAG AGCTCGAAGG CACGTGCGTC GAGCTCCGGA GCACTCGGTG 
TCCCATTTGT TATGGATCTT CCATCCAGCG ACAAGAAAGT TCATGTCAGA TGCGAAAAGG 
TTGGAGCATG TGTTGAGCTC GAAGCAGAAG CAGTTGAGAT TTGGGTCTTT TGTGGAAGAT 
GAGCAATTAT CCCCACTTGC TCGAATCCGA ACAACGCTGA AAGTGTACCT ACTCTCCAAA 
CTTGTACAGG AACTTGTCGG TGGTGACGAG ATCTTTACAA AAAATGTGGA ACGCATCCTA 
AATGACAATG ACCGTCTCGA TGATGAAGTA GACGTGGTTG ATGTTTCAAG ACTTTTGGTG 
ACAATTTCAA CGCAGTGCGC TGCCATTTTG ACTAATGAGA AGGATGAGTC AGCGAAATTC 
GGAACCTGGA TCTCTCGAAA CGGAGATGCT TGTTGCACAT GGTGGACGCA CGTTCTGACA 
TGTGGAATCT ATTGGAGGAG TAACAAGAAT GAGCTGGCAC GGCAACACTA TTCACTGATC 
AGGAACTGTC CGCCGAAGAT TTTGACAGAC AATCTGGGTT TGGCGGTTGG CCACGCGTTG 
TGTGCTCGCA AGATTTGCAT AGATGACCGA GATTCCCCGA AAGTCAGTCA ATACGTGTGC 
ATTCACACAA AGAAGTCGCT CGAATCCCTC CGACTATTCT CCACATCATC GCGAGCATCA 
GGTGTGGTGT CTGGAATTCA GGAAGGTACA CGCCGAATGG CCTACGAATG GATTATGAAC 
TCGCTGCTCG ACGCGTGGCG TTCCAATCTA TTCGCATCGA AACCCTACTG GACACAAAGC 
TTCAAGGGAC AATCCACGTT TAGTACGCTT TATCAAGAGG CGTATAATCA TTATGCGATT 
ATTAATGGGA CAAGGGGAGA TTGTTGGAGA CTATTTGTCT ACGAGCTCAC GTGCCGAATG 
CTCAACGGAG CCAACCCACA AGCCACGTGG TCAGGCGTCC GACGCGTTCG ATCTACAAAA 
ATGGACGCGG TCCGAGGAAG AGTGAGCATG CGACGCTCGG CTCAACCGGA CGCATTTCAT 
CTTCATACAC TGGTTAAACT ACATACTTCT ATGGATCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499472.1
CDS	1..3342
Translation	MNEEFEGDVPMSDPFLSLVTKLDDIAPFPNNDPLDFDMEHNWQEPGPSQQPDPSIPGNQHSPPQEYYDIDGQRDVSTLHSLLNHNNDDFFSMRFSPPNFDLGGGRGPSLAATQQLSGEGPASMLNPLQTSPPSGGYPPADAYRPLSLAQQLAAPAMTPHQAASLFVNTNGIDQKNFTHAMLSPPHHTSMTPQPYTEAMEHINGYMSPYDQAQGPSGPSYYSQHHQSPPPHHHHHHPMPKIHENPEQVASPSIEDAPETKPTHLVEPQSPKSPQNMKEELLRLLVNMSPSEVERLKNKKSGACSATNGPSRSKEKAAKIVIQETAEGDEDEDDEDSDSGETMSQGTTIIVRRPKTERRTAHNLIEKKYRCSINDRIQQLKVLLCGDEAKLSKSATLRRAIEHIEEVEHENQVLKHHVEQMRKTLQNNRLPYPEPIQYTEYSARSPVESSPSPPRNERKRSRMSTTTPMKNGTRDGSSKVTLFAMLLAVLIFNPIGLLAGSAIFSKAAAEAPIASPFEHGRVIDDPDGTSTRTLFWEGSIINMSYVWVFNILMIIYVVVKLLIHGDPVQDFMSVSWQTFVTTREKARAELNSGNLKDAQRKFCECLATLDRSLPSPGVDSVFSVGWECVRHLLNWLWIGRYIARRRRSTTKPVSVVCRSHAQTAVLYHEIHQLHLMGITGNFEDTYEPSALTGLFMSLCAVNLAEAAGASNDGLPRAVMAQIYISASIQCRLALPNLLAPFFSGYFLRRARRHVRRAPEHSVSHLLWIFHPATRKFMSDAKRLEHVLSSKQKQLRFGSFVEDEQLSPLARIRTTLKVYLLSKLVQELVGGDEIFTKNVERILNDNDRLDDEVDVVDVSRLLVTISTQCAAILTNEKDESAKFGTWISRNGDACCTWWTHVLTCGIYWRSNKNELARQHYSLIRNCPPKILTDNLGLAVGHALCARKICIDDRDSPKVSQYVCIHTKKSLESLRLFSTSSRASGVVSGIQEGTRRMAYEWIMNSLLDAWRSNLFASKPYWTQSFKGQSTFSTLYQEAYNHYAIINGTRGDCWRLFVYELTCRMLNGANPQATWSGVRRVRSTKMDAVRGRVSMRRSAQPDAFHLHTLVKLHTSMDL

Target ORF information:

RefSeq Version	NM_067071.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Sterol regulatory element Binding Protein (sbp-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_067071.5

ORF Insert Sequence:

ATGAACGAAG AATTCGAGGG AGACGTCCCT ATGTCGGATC CGTTTCTCTC ATTGGTCACA 
AAATTGGATG ATATTGCGCC ATTTCCAAAT AACGACCCGC TCGATTTTGA CATGGAGCAC 
AACTGGCAAG AGCCCGGACC ATCACAACAA CCGGATCCAT CAATTCCCGG AAATCAACAC 
AGTCCGCCAC AGGAATATTA TGATATTGAT GGTCAACGAG ACGTAAGCAC CTTACACTCC 
CTGCTCAACC ACAACAACGA CGACTTCTTC TCAATGCGAT TTTCCCCGCC AAACTTTGAT 
CTCGGCGGAG GCCGTGGACC TTCTCTAGCC GCCACCCAAC AATTATCTGG AGAAGGTCCT 
GCAAGTATGC TTAACCCCTT ACAAACATCT CCACCAAGTG GAGGTTACCC CCCGGCAGAT 
GCCTACAGAC CTCTATCACT TGCTCAACAA CTCGCCGCGC CAGCGATGAC TCCACATCAG 
GCAGCGTCGC TTTTTGTTAA TACTAATGGA ATTGATCAAA AGAATTTCAC TCATGCAATG 
CTATCTCCAC CACACCATAC CTCAATGACT CCTCAACCAT ATACAGAAGC AATGGAACAT 
ATCAACGGGT ACATGTCTCC ATACGACCAA GCTCAAGGCC CATCAGGACC ATCATATTAC 
TCACAACACC ATCAATCTCC ACCACCTCAT CACCACCATC ACCACCCGAT GCCAAAAATC 
CATGAGAACC CTGAACAAGT GGCATCTCCA TCGATTGAAG ATGCTCCAGA GACGAAACCA 
ACTCATTTGG TTGAACCACA AAGTCCAAAA AGCCCGCAGA ATATGAAAGA GGAGCTTCTT 
CGGTTACTAG TTAACATGTC TCCGAGTGAA GTTGAACGGT TAAAGAATAA AAAATCAGGA 
GCATGTTCAG CGACGAATGG GCCATCGAGG AGTAAGGAGA AGGCGGCGAA GATTGTGATT 
CAGGAGACAG CGGAAGGGGA TGAAGATGAG GATGATGAGG ATAGTGATTC CGGGGAGACT 
ATGTCTCAGG GAACTACTAT TATTGTTCGA AGACCAAAAA CCGAGCGTCG TACGGCACAC 
AATCTCATCG AAAAGAAGTA TAGATGCTCA ATAAATGATC GAATTCAACA GCTGAAAGTA 
CTTTTGTGTG GGGATGAAGC TAAGCTTTCA AAATCGGCAA CACTACGACG GGCTATTGAA 
CATATCGAGG AGGTTGAACA CGAGAATCAG GTGTTGAAGC ATCATGTTGA ACAAATGAGA 
AAGACACTGC AGAATAATCG ATTACCGTAC CCGGAACCAA TTCAATACAC TGAATACTCT 
GCCCGATCAC CCGTCGAATC ATCTCCTTCT CCACCTAGAA ATGAGAGAAA ACGATCACGA 
ATGAGCACAA CGACTCCTAT GAAGAATGGA ACTAGAGATG GATCTTCGAA AGTTACCCTT 
TTTGCGATGC TCCTAGCAGT TCTGATTTTT AATCCGATTG GATTGCTCGC TGGAAGTGCG 
ATATTCTCAA AAGCCGCTGC AGAAGCTCCG ATTGCCTCCC CGTTCGAGCA TGGAAGAGTG 
ATTGATGACC CGGATGGAAC TAGCACTCGG ACGCTTTTCT GGGAAGGGAG TATCATCAAT 
ATGAGCTATG TCTGGGTGTT CAACATCTTA ATGATCATAT ATGTGGTTGT CAAACTGCTG 
ATCCATGGTG ACCCTGTTCA AGACTTCATG TCCGTTTCAT GGCAGACTTT TGTGACGACT 
CGAGAGAAGG CGAGAGCCGA GTTGAACTCT GGAAATTTGA AAGATGCTCA GAGAAAGTTC 
TGCGAGTGTC TTGCAACGTT GGATCGATCG CTTCCATCAC CGGGGGTTGA TTCGGTGTTT 
TCGGTTGGCT GGGAATGCGT TCGACATCTT TTGAATTGGT TGTGGATCGG GAGATACATC 
GCAAGAAGGC GCAGGTCCAC CACGAAGCCT GTCTCAGTCG TTTGTAGGAG TCATGCGCAG 
ACTGCAGTTC TCTATCATGA AATTCATCAG CTCCATCTAA TGGGTATCAC TGGAAACTTC 
GAAGACACCT ATGAACCATC CGCCCTAACG GGCCTCTTCA TGTCCCTCTG TGCAGTAAAC 
CTTGCTGAAG CTGCCGGAGC ATCAAACGAC GGACTTCCAC GCGCCGTCAT GGCTCAGATC 
TACATTTCTG CATCCATCCA ATGCCGTTTG GCTCTTCCGA ACCTACTCGC ACCATTCTTC 
TCGGGATACT TTTTACGAAG AGCTCGAAGG CACGTGCGTC GAGCTCCGGA GCACTCGGTG 
TCCCATTTGT TATGGATCTT CCATCCAGCG ACAAGAAAGT TCATGTCAGA TGCGAAAAGG 
TTGGAGCATG TGTTGAGCTC GAAGCAGAAG CAGTTGAGAT TTGGGTCTTT TGTGGAAGAT 
GAGCAATTAT CCCCACTTGC TCGAATCCGA ACAACGCTGA AAGTGTACCT ACTCTCCAAA 
CTTGTACAGG AACTTGTCGG TGGTGACGAG ATCTTTACAA AAAATGTGGA ACGCATCCTA 
AATGACAATG ACCGTCTCGA TGATGAAGTA GACGTGGTTG ATGTTTCAAG ACTTTTGGTG 
ACAATTTCAA CGCAGTGCGC TGCCATTTTG ACTAATGAGA AGGATGAGTC AGCGAAATTC 
GGAACCTGGA TCTCTCGAAA CGGAGATGCT TGTTGCACAT GGTGGACGCA CGTTCTGACA 
TGTGGAATCT ATTGGAGGAG TAACAAGAAT GAGCTGGCAC GGCAACACTA TTCACTGATC 
AGGAACTGTC CGCCGAAGAT TTTGACAGAC AATCTGGGTT TGGCGGTTGG CCACGCGTTG 
TGTGCTCGCA AGATTTGCAT AGATGACCGA GATTCCCCGA AAGTCAGTCA ATACGTGTGC 
ATTCACACAA AGAAGTCGCT CGAATCCCTC CGACTATTCT CCACATCATC GCGAGCATCA 
GGTGTGGTGT CTGGAATTCA GGAAGGTACA CGCCGAATGG CCTACGAATG GATTATGAAC 
TCGCTGCTCG ACGCGTGGCG TTCCAATCTA TTCGCATCGA AACCCTACTG GACACAAAGC 
TTCAAGGGAC AATCCACGTT TAGTACGCTT TATCAAGAGG CGTATAATCA TTATGCGATT 
ATTAATGGGA CAAGGGGAGA TTGTTGGAGA CTATTTGTCT ACGAGCTCAC GTGCCGAATG 
CTCAACGGAG CCAACCCACA AGCCACGTGG TCAGGCGTCC GACGCGTTCG ATCTACAAAA 
ATGGACGCGG TCCGAGGAAG AGTGAGCATG CGACGCTCGG CTCAACCGGA CGCATTTCAT 
CTTCATACAC TGGTTAAACT ACATACTTCT ATGGATCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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sbp-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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