gpr-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	gpr-1
Entrez Gene ID	176210
Full Name	G-protein regulator 1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_066499.4	NP_498900.1	G-protein regulator 1
NM_066499.3	NP_498900.1	G-protein regulator 1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	gpr-1	NP_498900.1
Caenorhabditis elegans (roundworm)	gpr-2	NP_499066.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Four distinct in vivo phosphorylated LIN-5 residues were found to have critical functions in spindle positioning. Two of these residues form part of a 30 amino acid binding site for GPR-1, which we identified by reverse two-hybrid screening. These LIN-5 phosphorylations promote LIN-5-GPR-1/2 interaction and contribute to cortical pulling forces
Title: Multisite Phosphorylation of NuMA-Related LIN-5 Controls Mitotic Spindle Positioning in C. elegans.

Progeny of these animals (F2) inherit either only F0 maternal or only F0 paternal chromosomes, and thus descend from only either of their grandparents' gametes.
Title: Engineered non-Mendelian inheritance of entire parental genomes in C. elegans.

GPR-1/2 can sense mechanical properties of the cortex, which may be important for GPR-1/2 function regulating spindle positioning and cytokinesis.
Title: G protein regulator 1 (GPR-1) localizes to cortical sites of artificial mechanical indentation in Caenorhabditis elegans zygotes.

Data demonstrate that GPB-1 depletion results in the loss of GPR-1/2 asymmetry during mitosis.
Title: Polarity mediates asymmetric trafficking of the Gbeta heterotrimeric G-protein subunit GPB-1 in C. elegans embryos.

Dynamic localization of gpr-1 to cell cortex forces generation of domains during spindle positioning.
Title: Dynamic localization of LIN-5 and GPR-1/2 to cortical force generation domains during spindle positioning.

The following gpr-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gpr-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340804	NM_066499.4 Latest version!	Caenorhabditis elegans G-protein regulator 1 (gpr-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OCa340804	NM_066499.3	Caenorhabditis elegans G-protein regulator 1 (gpr-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gpr-1 ( NM_066499.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498900.1 Caenorhabditis elegans G-protein regulator 1 (gpr-1), mRNA.

Price & Availability↑

CloneID	OCa340804
Clone ID Related Accession (Same CDS sequence)	NM_066499.4 , NM_066499.3
Accession Version	NM_066499.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	G-protein regulator 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_066499.3.

ATGGACGTCT CTTATTACGA TGGTCCCAAG GATGAAGTCG CCGAAGCAAT GCTGAAAAGC 
GCGGTGACGG CCATGAGATT GGGACAATAC GAGGATGGAA AAGGACGCTT AGAGGAGATA 
ATGGAGTTCG GAACCTCAAA TTTTCAACTA CTTGGTACAA TCTACATGTA TTACGGAAGA 
GTGTGCAGGC ATTTGAACCA TGATGCCAAG GCCTTGGAGT TTTTCGAACA TGAGTTGAAC 
ATGTTCAAAT TGATCTTCAA CTACCCAGAA GCATGTGATT CCACACGTCG CATCGTCGAG 
CAGGCACTCA AAATGGGAAA GTTCCCCAAG GCTCGACGGT TTGCTGAGGA TCTCATTGAT 
TACACCAGCA ATAAGAAGAA CGGAGAGAAG TATATCGGTC AAGCTCGAAT TTTGTTCGCT 
TCCGTGTGCC TCGAAGGATG TGAAAGAGAC GTCGAGAGTA ATCAAGATGA GAAGAAGAAG 
CTTTTGTCAA TATGTGCTGA ACAGATTGCA GCCGTGAAAT TGTTCAACGA GAATAATACG 
GAAGGAGCTG TGTCTGAGAC CAAAATCATG TTACTTGAGG CGAAATGCTT GTCACTAGAC 
GAAAAATACG AGGAATCGCG TCGCAAGTAT CAAGAATGCA TCGATTTTGC CATCAAAACA 
GACCAGTTTG AAGCAGTTCA CATCGCCTAT TACGACAAGG CTCTATATGC TGAGACAGAT 
CTTCTTTTCT TTATTATCAG AGATCTCAGA AGTGCTCTCT TCTACGCCAC GAAATTCGGA 
AAAGAGCGAG ATGTAGTCAA ATATAAGTCG AAGCTATCCG AAGAGATGCT GAGAAATGGC 
GAATTCCACG AAGCATATCT CTACGGATTG GAAGCGCTTG TATCGATTCG GAAGCTTGGA 
TTGAACGAAT ACATTGGAGA TGTGTTGCTT ACAATCGCAA AGTGCCTCAT TGCACTTGGA 
AAAAGACGCC AAGCTGCTTA TTTTATCATC TTGGGGAGTG TTCTGACCAT CAACCAAAAC 
AGTTTCAAAC TGTTCTACGA GCAGATCGAC GTGGCGATGA ATCAAGAGAG AAGCGAAACG 
GCAACTGATC AAGATGTATG CCTCGCAATT GATTCGTCTC CTGATCCGAC ATCCTCGAAT 
GACATGATTA ATAAGTTCGT CGTCGAACTG GAGCACGCAA CAAATGTGGA AACCTGGGAA 
ATGATTGTCA ACGGAATCAT TGACGACCAG AAGAAACCAG TGGCGATCGA AAAGAAAGAG 
AACGAGGAAC CCGTAGACAT GATGGATCTC ATTTTCAGTA TGAGCTCACG TATGGATGAT 
CAAAGAACTG AACTGCCTGC TGCCAGATTC ATTCCGCCTC GTCCAGTGTC ATCGGCATCG 
AAAAAGACTA CAAAGAGTCA CAGAATCCTC CCTGGACTCC GTGCCAATTG GACAAAAGTG 
CAGTCGATGA AGTTCGATGG TCACACAATG AATAGGATCC TGAAGAGGTC GAAGAAAAGC 
AAATCGTCAT TGGATTCTAC AAATTCGATG CAGGGCGATG ATACTCGAAG CGATGATGTG 
ACAATGACGT CCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498900.1
CDS	32..1609
Translation	MDVSYYDGPKDEVAEAMLKSAVTAMRLGQYEDGKGRLEEIMEFGTSNFQLLGTIYMYYGRVCRHLNHDAKALEFFEHELNMFKLIFNYPEACDSTRRIVEQALKMGKFPKARRFAEDLIDYTSNKKNGEKYIGQARILFASVCLEGCERDVESNQDEKKKLLSICAEQIAAVKLFNENNTEGAVSETKIMLLEAKCLSLDEKYEESRRKYQECIDFAIKTDQFEAVHIAYYDKALYAETDLLFFIIRDLRSALFYATKFGKERDVVKYKSKLSEEMLRNGEFHEAYLYGLEALVSIRKLGLNEYIGDVLLTIAKCLIALGKRRQAAYFIILGSVLTINQNSFKLFYEQIDVAMNQERSETATDQDVCLAIDSSPDPTSSNDMINKFVVELEHATNVETWEMIVNGIIDDQKKPVAIEKKENEEPVDMMDLIFSMSSRMDDQRTELPAARFIPPRPVSSASKKTTKSHRILPGLRANWTKVQSMKFDGHTMNRILKRSKKSKSSLDSTNSMQGDDTRSDDVTMTSK

Target ORF information:

RefSeq Version	NM_066499.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans G-protein regulator 1 (gpr-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066499.4

ORF Insert Sequence:

ATGGACGTCT CTTATTACGA TGGTCCCAAG GATGAAGTCG CCGAAGCAAT GCTGAAAAGC 
GCGGTGACGG CCATGAGATT GGGACAATAC GAGGATGGAA AAGGACGCTT AGAGGAGATA 
ATGGAGTTCG GAACCTCAAA TTTTCAACTA CTTGGTACAA TCTACATGTA TTACGGAAGA 
GTGTGCAGGC ATTTGAACCA TGATGCCAAG GCCTTGGAGT TTTTCGAACA TGAGTTGAAC 
ATGTTCAAAT TGATCTTCAA CTACCCAGAA GCATGTGATT CCACACGTCG CATCGTCGAG 
CAGGCACTCA AAATGGGAAA GTTCCCCAAG GCTCGACGGT TTGCTGAGGA TCTCATTGAT 
TACACCAGCA ATAAGAAGAA CGGAGAGAAG TATATCGGTC AAGCTCGAAT TTTGTTCGCT 
TCCGTGTGCC TCGAAGGATG TGAAAGAGAC GTCGAGAGTA ATCAAGATGA GAAGAAGAAG 
CTTTTGTCAA TATGTGCTGA ACAGATTGCA GCCGTGAAAT TGTTCAACGA GAATAATACG 
GAAGGAGCTG TGTCTGAGAC CAAAATCATG TTACTTGAGG CGAAATGCTT GTCACTAGAC 
GAAAAATACG AGGAATCGCG TCGCAAGTAT CAAGAATGCA TCGATTTTGC CATCAAAACA 
GACCAGTTTG AAGCAGTTCA CATCGCCTAT TACGACAAGG CTCTATATGC TGAGACAGAT 
CTTCTTTTCT TTATTATCAG AGATCTCAGA AGTGCTCTCT TCTACGCCAC GAAATTCGGA 
AAAGAGCGAG ATGTAGTCAA ATATAAGTCG AAGCTATCCG AAGAGATGCT GAGAAATGGC 
GAATTCCACG AAGCATATCT CTACGGATTG GAAGCGCTTG TATCGATTCG GAAGCTTGGA 
TTGAACGAAT ACATTGGAGA TGTGTTGCTT ACAATCGCAA AGTGCCTCAT TGCACTTGGA 
AAAAGACGCC AAGCTGCTTA TTTTATCATC TTGGGGAGTG TTCTGACCAT CAACCAAAAC 
AGTTTCAAAC TGTTCTACGA GCAGATCGAC GTGGCGATGA ATCAAGAGAG AAGCGAAACG 
GCAACTGATC AAGATGTATG CCTCGCAATT GATTCGTCTC CTGATCCGAC ATCCTCGAAT 
GACATGATTA ATAAGTTCGT CGTCGAACTG GAGCACGCAA CAAATGTGGA AACCTGGGAA 
ATGATTGTCA ACGGAATCAT TGACGACCAG AAGAAACCAG TGGCGATCGA AAAGAAAGAG 
AACGAGGAAC CCGTAGACAT GATGGATCTC ATTTTCAGTA TGAGCTCACG TATGGATGAT 
CAAAGAACTG AACTGCCTGC TGCCAGATTC ATTCCGCCTC GTCCAGTGTC ATCGGCATCG 
AAAAAGACTA CAAAGAGTCA CAGAATCCTC CCTGGACTCC GTGCCAATTG GACAAAAGTG 
CAGTCGATGA AGTTCGATGG TCACACAATG AATAGGATCC TGAAGAGGTC GAAGAAAAGC 
AAATCGTCAT TGGATTCTAC AAATTCGATG CAGGGCGATG ATACTCGAAG CGATGATGTG 
ACAATGACGT CCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gpr-1 ( NM_066499.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498900.1 Caenorhabditis elegans G-protein regulator 1 (gpr-1), partial mRNA.

Price & Availability↑

CloneID	OCa340804
Clone ID Related Accession (Same CDS sequence)	NM_066499.4 , NM_066499.3
Accession Version	NM_066499.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	G-protein regulator 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_066499.2. COMPLETENESS: incomplete on both ends.

ATGGACGTCT CTTATTACGA TGGTCCCAAG GATGAAGTCG CCGAAGCAAT GCTGAAAAGC 
GCGGTGACGG CCATGAGATT GGGACAATAC GAGGATGGAA AAGGACGCTT AGAGGAGATA 
ATGGAGTTCG GAACCTCAAA TTTTCAACTA CTTGGTACAA TCTACATGTA TTACGGAAGA 
GTGTGCAGGC ATTTGAACCA TGATGCCAAG GCCTTGGAGT TTTTCGAACA TGAGTTGAAC 
ATGTTCAAAT TGATCTTCAA CTACCCAGAA GCATGTGATT CCACACGTCG CATCGTCGAG 
CAGGCACTCA AAATGGGAAA GTTCCCCAAG GCTCGACGGT TTGCTGAGGA TCTCATTGAT 
TACACCAGCA ATAAGAAGAA CGGAGAGAAG TATATCGGTC AAGCTCGAAT TTTGTTCGCT 
TCCGTGTGCC TCGAAGGATG TGAAAGAGAC GTCGAGAGTA ATCAAGATGA GAAGAAGAAG 
CTTTTGTCAA TATGTGCTGA ACAGATTGCA GCCGTGAAAT TGTTCAACGA GAATAATACG 
GAAGGAGCTG TGTCTGAGAC CAAAATCATG TTACTTGAGG CGAAATGCTT GTCACTAGAC 
GAAAAATACG AGGAATCGCG TCGCAAGTAT CAAGAATGCA TCGATTTTGC CATCAAAACA 
GACCAGTTTG AAGCAGTTCA CATCGCCTAT TACGACAAGG CTCTATATGC TGAGACAGAT 
CTTCTTTTCT TTATTATCAG AGATCTCAGA AGTGCTCTCT TCTACGCCAC GAAATTCGGA 
AAAGAGCGAG ATGTAGTCAA ATATAAGTCG AAGCTATCCG AAGAGATGCT GAGAAATGGC 
GAATTCCACG AAGCATATCT CTACGGATTG GAAGCGCTTG TATCGATTCG GAAGCTTGGA 
TTGAACGAAT ACATTGGAGA TGTGTTGCTT ACAATCGCAA AGTGCCTCAT TGCACTTGGA 
AAAAGACGCC AAGCTGCTTA TTTTATCATC TTGGGGAGTG TTCTGACCAT CAACCAAAAC 
AGTTTCAAAC TGTTCTACGA GCAGATCGAC GTGGCGATGA ATCAAGAGAG AAGCGAAACG 
GCAACTGATC AAGATGTATG CCTCGCAATT GATTCGTCTC CTGATCCGAC ATCCTCGAAT 
GACATGATTA ATAAGTTCGT CGTCGAACTG GAGCACGCAA CAAATGTGGA AACCTGGGAA 
ATGATTGTCA ACGGAATCAT TGACGACCAG AAGAAACCAG TGGCGATCGA AAAGAAAGAG 
AACGAGGAAC CCGTAGACAT GATGGATCTC ATTTTCAGTA TGAGCTCACG TATGGATGAT 
CAAAGAACTG AACTGCCTGC TGCCAGATTC ATTCCGCCTC GTCCAGTGTC ATCGGCATCG 
AAAAAGACTA CAAAGAGTCA CAGAATCCTC CCTGGACTCC GTGCCAATTG GACAAAAGTG 
CAGTCGATGA AGTTCGATGG TCACACAATG AATAGGATCC TGAAGAGGTC GAAGAAAAGC 
AAATCGTCAT TGGATTCTAC AAATTCGATG CAGGGCGATG ATACTCGAAG CGATGATGTG 
ACAATGACGT CCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498900.1
CDS	1..1578
Translation	MDVSYYDGPKDEVAEAMLKSAVTAMRLGQYEDGKGRLEEIMEFGTSNFQLLGTIYMYYGRVCRHLNHDAKALEFFEHELNMFKLIFNYPEACDSTRRIVEQALKMGKFPKARRFAEDLIDYTSNKKNGEKYIGQARILFASVCLEGCERDVESNQDEKKKLLSICAEQIAAVKLFNENNTEGAVSETKIMLLEAKCLSLDEKYEESRRKYQECIDFAIKTDQFEAVHIAYYDKALYAETDLLFFIIRDLRSALFYATKFGKERDVVKYKSKLSEEMLRNGEFHEAYLYGLEALVSIRKLGLNEYIGDVLLTIAKCLIALGKRRQAAYFIILGSVLTINQNSFKLFYEQIDVAMNQERSETATDQDVCLAIDSSPDPTSSNDMINKFVVELEHATNVETWEMIVNGIIDDQKKPVAIEKKENEEPVDMMDLIFSMSSRMDDQRTELPAARFIPPRPVSSASKKTTKSHRILPGLRANWTKVQSMKFDGHTMNRILKRSKKSKSSLDSTNSMQGDDTRSDDVTMTSK

Target ORF information:

RefSeq Version	NM_066499.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans G-protein regulator 1 (gpr-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066499.3

ORF Insert Sequence:

ATGGACGTCT CTTATTACGA TGGTCCCAAG GATGAAGTCG CCGAAGCAAT GCTGAAAAGC 
GCGGTGACGG CCATGAGATT GGGACAATAC GAGGATGGAA AAGGACGCTT AGAGGAGATA 
ATGGAGTTCG GAACCTCAAA TTTTCAACTA CTTGGTACAA TCTACATGTA TTACGGAAGA 
GTGTGCAGGC ATTTGAACCA TGATGCCAAG GCCTTGGAGT TTTTCGAACA TGAGTTGAAC 
ATGTTCAAAT TGATCTTCAA CTACCCAGAA GCATGTGATT CCACACGTCG CATCGTCGAG 
CAGGCACTCA AAATGGGAAA GTTCCCCAAG GCTCGACGGT TTGCTGAGGA TCTCATTGAT 
TACACCAGCA ATAAGAAGAA CGGAGAGAAG TATATCGGTC AAGCTCGAAT TTTGTTCGCT 
TCCGTGTGCC TCGAAGGATG TGAAAGAGAC GTCGAGAGTA ATCAAGATGA GAAGAAGAAG 
CTTTTGTCAA TATGTGCTGA ACAGATTGCA GCCGTGAAAT TGTTCAACGA GAATAATACG 
GAAGGAGCTG TGTCTGAGAC CAAAATCATG TTACTTGAGG CGAAATGCTT GTCACTAGAC 
GAAAAATACG AGGAATCGCG TCGCAAGTAT CAAGAATGCA TCGATTTTGC CATCAAAACA 
GACCAGTTTG AAGCAGTTCA CATCGCCTAT TACGACAAGG CTCTATATGC TGAGACAGAT 
CTTCTTTTCT TTATTATCAG AGATCTCAGA AGTGCTCTCT TCTACGCCAC GAAATTCGGA 
AAAGAGCGAG ATGTAGTCAA ATATAAGTCG AAGCTATCCG AAGAGATGCT GAGAAATGGC 
GAATTCCACG AAGCATATCT CTACGGATTG GAAGCGCTTG TATCGATTCG GAAGCTTGGA 
TTGAACGAAT ACATTGGAGA TGTGTTGCTT ACAATCGCAA AGTGCCTCAT TGCACTTGGA 
AAAAGACGCC AAGCTGCTTA TTTTATCATC TTGGGGAGTG TTCTGACCAT CAACCAAAAC 
AGTTTCAAAC TGTTCTACGA GCAGATCGAC GTGGCGATGA ATCAAGAGAG AAGCGAAACG 
GCAACTGATC AAGATGTATG CCTCGCAATT GATTCGTCTC CTGATCCGAC ATCCTCGAAT 
GACATGATTA ATAAGTTCGT CGTCGAACTG GAGCACGCAA CAAATGTGGA AACCTGGGAA 
ATGATTGTCA ACGGAATCAT TGACGACCAG AAGAAACCAG TGGCGATCGA AAAGAAAGAG 
AACGAGGAAC CCGTAGACAT GATGGATCTC ATTTTCAGTA TGAGCTCACG TATGGATGAT 
CAAAGAACTG AACTGCCTGC TGCCAGATTC ATTCCGCCTC GTCCAGTGTC ATCGGCATCG 
AAAAAGACTA CAAAGAGTCA CAGAATCCTC CCTGGACTCC GTGCCAATTG GACAAAAGTG 
CAGTCGATGA AGTTCGATGG TCACACAATG AATAGGATCC TGAAGAGGTC GAAGAAAAGC 
AAATCGTCAT TGGATTCTAC AAATTCGATG CAGGGCGATG ATACTCGAAG CGATGATGTG 
ACAATGACGT CCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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gpr-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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