polq-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	polq-1
Entrez Gene ID	175810
Full Name	DNA polymerase theta
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_065849.4	NP_498250.3	DNA polymerase theta
NM_065849.5	NP_498250.3	DNA polymerase theta

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our study provides a step-wise and versatile model for the in vivo mechanism of POLQ action, which explains the molecular nature of mutagen-induced deletion alleles
Title: Genomic Scars Generated by Polymerase Theta Reveal the Versatile Mechanism of Alternative End-Joining.

POLQ is a major driver of inheritable genomic alterations in Caenorhabditis elegans.
Title: Polymerase ? is a key driver of genome evolution and of CRISPR/Cas9-mediated mutagenesis.

non-canonical DNA break repair mechanism generates deletions characterized by an extremely narrow size distribution
Title: A Polymerase Theta-dependent repair pathway suppresses extensive genomic instability at endogenous G4 DNA sites.

The following polq-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the polq-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa341652	NM_065849.5 Latest version!	Caenorhabditis elegans DNA polymerase theta (polq-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OCa341652	NM_065849.4	Caenorhabditis elegans DNA polymerase theta (polq-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

polq-1 ( NM_065849.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498250.3 Caenorhabditis elegans DNA polymerase theta (polq-1), partial mRNA.

Price & Availability↑

CloneID	OCa341652
Clone ID Related Accession (Same CDS sequence)	NM_065849.4 , NM_065849.5
Accession Version	NM_065849.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	DNA polymerase theta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_065849.4. COMPLETENESS: incomplete on the 5' end.

ATGGACGAGG CTTCCTGTAG ATTTCCAGAA TGGGTTTCTT CAAAAATCAT CGATTATTAC 
GCAGAGCAGA ACATCAAAGC GCTGTTCGAT TGGCAGATTG ATGTGTTGAA TGAGGCAAGA 
CAATTTGAAG ATCAACACTT GATATTCAGT GCGCCAACAA GTGCTGGGAA AAGCATTGTA 
GCTGAACTTT TATCATGGAA AGTCGCTTCA ACTGGTAGAA AGGTACTCTT CGTACTTCCT 
TATATCTCCG TGGCTCGAGA GAAACTTCAT CAAATTCAAC GGTGTTGGAG ACGAGATGAC 
ATTTCTGTCT GTGGCTTCAT TGGTCCACAG GCCTCAAATC CAAATGAGTG GCTTGGGGCA 
GTTTGTACCA TCGAAAAAGC AGCAAGTTTG ACAAATCGAG CATTATCAGA AGATTGGTTT 
GAGGAAATTG GTATGATAGT CGTTGATGAA ATGCACATGG TTTTCGATTC GTCTCGTGGT 
GCACACATTG AGCACATGCT CTCGAAAGTT TTGCTCTGGA ACCAATCTGC ACTGGAAAAA 
GTTAGAATTA TCGGAATGAG TGCAACTATT CCAGAACTTT ATCGAATAGG CAAATGGCTG 
GACGGTGCGA AAGTTTTCGA AGCAAGATTT CGACCAATTG TTCTACAAAA TCATATTGTA 
ATTGGTTCGG AGTTGAGGAA ATCTGGAGAT AACAAAGTTC TAAGAGAATT CTCTGAAGAT 
CCATTAATAT TGCTTACCGA AGAGAGTTTT CGCCGCAATT CTCAGACTTT GGTAATGATT 
TCTTCGAAAC TTGATGCCGA GAAGACTGCT TTGAATATTG CGTCGAGATT CCATGAGATC 
AATAAAACCG ACAGCTCATT GTTGGAAATA TTAAAAGAAC GAGCCAATGG ACTATTATTT 
ATAAAGCATG GATTGGAACG GAATGGTTGC AAGGATCGGA ATGTTATGTC AACACTTGCA 
TGGGGAGTAG CATATCATCA TGCTGGATTG ACGATGGAGG AAAGAGAATG CATTGAACTA 
GGATTTCGGG AGAAAAATAT TGTTATTCTT GTGGCTACAA GTACTTTGGC TTCAGGCGTA 
AATCTCCCTG CGGAACGAGT ACTCATCAAG GCTCAGCCAC GTGGCCCATC GGCTCTCACT 
TCTCTAAATT ACCGTCAGAT GGTGGGAAGA GCGGGAAGAA CAGGACATGC TACACGAGGT 
GAAACCTATC TTCTCATCAA GAAATGTGAT CGAGATGCCG TGCTGAAAAT CATCGAGACT 
CCGATTGATC AAGGTGTTCT AACAAGGAAA CGAGATGCTG AACGGACTAA TTTAAGCAGA 
TTTATATTAG AGGGAATCTG CACTGGGCTC ACGACAACAA GATCTCAGAT TCATGATCTC 
TGCAAGCTTC TCCTATTTAA TTCTGAAAAC CTTCAACTTT CTGACATTGC CATCGAGATG 
CTTCTTCGGA ATTCTTTCAT CTCCCAAGAC GAAAACGATG ATCAATTAAG TCCCACACAA 
CTCGGAAGAG CTGCAATTGC TTCATCTCTA CCACCTGAAG CATCACTTGC CATTTTTGAA 
GATTTGAATT CGGCGAGTCG AGCAATTGCA CTGGATACTG AGCTTCATAT GCTCTATTTG 
GTATATTTTT ACAAGAATTC GAGGGCCCAA ATAATTCAAA AAATATTCAA AATTTATTCA 
ATTTTCATTT TAAAAAAGTT TAAAAATTTA GAACCTAAAT TCAAAAAAAA AATTTCTGAA 
AACATCACCG TACATATTAC AAATTCTATA AGAAAAAAAC AACATTTTTG GCATGTAACC 
CCAATCAACG TAAGTGTTTG GCAGGAATGT GATTGGCATC ACCTTTTCTC AATTTTCTCG 
AAACTTCCAT CAGATCATAA ACGAATCGCA AAGTTGGTTG GAGTCAGTGA AAAATTTATT 
TTGGATCAAC TTCAAGGACG AAGAAATGAC AAATTGCTTC AAATTCATAT TCGATTCTTT 
TCTGCGTTGG CACTTTTTGA TTTGATCAGC GAAATGAGCA TTTATGAGGT ATCTCACAAG 
TACCGAATTC CTCGTGGATG TCTTCAAACA CTCCAATCAC AGAGCGCCAC TTACGCGGCA 
ATGATTGTGG CTTTTTGCCT TCGACTGGGA TGGACATATC TCAAGGCACT GCTTGATGGA 
TTTGCAACGA GATTGTTGTT CGGTGTTAGA TCAGAGCTCT CGGAGCTTGT GGCGATTGAA 
GGAATTGATG GACAGAGAGC CCGAATCCTA CATGAACGTG GCGTGACGTG TCTGTCACAT 
TTGTCCGCTT GCGATTCTTC GAAACTTGCA CACTTTCTAA CATTGGCTGT TCCATACTCA 
AGCTCGAATT CAAACGATGG ACTCGGCGAG TGGCTTTTTG GAGAGCCAAG AATGAGAGTT 
GATGTGGCGG CTAGAACATT GAAAGAACGG GCTCGAAAAG TATTGATCAG AAGAGTGCAG 
GAGCTGGGAA TTTCTGTGGA GTTGCCGAAA TTTGAGGAGA ACGAGGAAAA TATTCAAGAG 
AGTTGTGATA GTGGACTTCC GGATAGTTGT GAAGGAATGG AAGATGAGTT GGAGGAGAAA 
GAGAATATTG TGAAGATGGA AGAAATGACA AAGAGTGTCA CGGAAATGTC ACTTACAGAT 
AATACAATAT CATTTAAAAG TGAGGATGAT TTGTTCAAAA AAGAGATTAA AGTAGAAGAA 
GATGAAGTTT TTATAAAAAA AGAAATAGAC GAAGATGAAG AAGAAATAGT TGAAGAAACT 
GTAATCGAGT GTCTAGAAAC ATCTCTTCTG AAACTAAAAG CATCAACAGA TGAAGTATTC 
CTAAGAAGGC TTTCACAAAC ATTCTCACCT ATTGGAAGAA GTCGCAGTAT TCTCAATAAC 
TCATTACTTG AAGATTCATT TGATAGACCA GTACCCAGAA GCTCTATTCC AATTCTTAAT 
TTCATCACTC CAAAAAGGGA ATCGCCAACT CCATATTTCG AAGACTCATT CGATCGTCCA 
ATACCTGGAT CCTTACCAAT AAGTTCTTCA AGACGAAAGT CAGTTCTTAC AAATATTGCT 
AATCTGGATT CAAGTCGTCG TGAATCGATT AATTCCAATG CTTCGGATAA CAATTCATTC 
GATGTTTTTG TAACTCCACC AACAAAATCT GCAAAAGAAG AAAAACGAAG AATTGCTGTA 
AAACATCCAC GAGTTGGAAA TATTATATAT TCACCACTCA CTTCCAGTCC AGTCATAAAG 
CATCCAAAAC TTGAAATCAA TCATTTTTAT TTAAAAGATG TTTGCCATGA TCATAATGCT 
TGGAATTTAT GGACAAAATC AAGTACATCT ACTTCGAGTT GTTCAATTCG AGTTTCAGAT 
GATTATACTG GAATTGCAAT TAGAACCGAT GCGGGGAATA CTTTTATTCC ATTACTTGAA 
ACGTTTGGAG GAGAGCCATC ACCTGCATCC AAGTACTTCG AGTCATTCTC CAAATGCATC 
ATCCCTCTGA ACACTCGCTT GGAATTCCTG AAAACACTTG CGGTTACTGT AGAAATGTAT 
ATTTCGTCTA TGGAAGATGC TTTCCTGATT TTTGAAAAGT TTGGAATCAA GATTTTCCGA 
CTGAAAGTTG TTCGAATTGC TGCGTATCTG AATAATGTTA TCGACGTGGA GCAGGAGGAA 
AACTCGAATT TTCTGCCAAT TCTAATGGAT CGTTATTCAA TTTTAGATCC GGAAATTAGA 
AAAACTTGCT CTTCGAGCCT GCACAAAGCT GCCGTTGAAG TATATTCCCT GAAGCCGATC 
TTCGAGAAAA TGTGCTGTTC TGGAGCAAGT TTACAATTAG AAATGGAATC TTGTCAGACT 
GTCTTGAACA TTTTCTACTC TGGCATCGTA TTTGATCAAG CTTTATGCAA TTCTTTCATC 
TATAAAATTA GAAAACAAAT CGAGAATTTG GAAGAAAATA TTTGGAGACT TGCTTACGGA 
AAATTCAATA TTCATTCTTC AAATGAAGTT GCAAATGTTC TATTCTACCG CCTTGGCCTA 
ATTTATCCAG AAACTTCCGG ATGTAAACCA AAACTACGAC ATCTTCCAAC AAATAAGCTT 
ATATTGGAAC AAATGAATAC TCAACATCCG ATTGTTGGAA AAATTCTTGA ATATCGACAA 
ATTCAACATA CATTGACACA ATGCTTGATG CCACTTGCCA AGTTCATTGG AAGAATTCAC 
TGTTGGTTTG AAATGTGTAC ATCGACTGGA AGGATTTTGA CAAGTGTTCC GAATTTGCAA 
AACGTTCCAA AGCGAATATC GTCCGATGGA ATGTCTGCCC GTCAACTTTT CATCGCAAAC 
TCTGAAAATT TGTTAATCGG AGCCGATTAC AAGCAGCTGG AGCTTCGTGT TCTGGCACAT 
TTGAGCAATG ATTCGAATCT TGTCAATTTA ATCACATCGG ATAGAGATTT ATTTGAAGAA 
CTCTCAATTC AATGGAATTT CCCACGAGAT GCTGTTAAAC AGCTTTGCTA TGGTTTGATC 
TATGGAATGG GCGCCAAAAG TTTATCGGAG TTGACAAGAA TGAGCATAGA AGATGCTGAA 
AAGATGCTAA AGGCGTTTTT TGCAATGTTC CCTGGTGTAA GAAGCTATAT CAACGAAACA 
AAGGAAAAAG TTTGCAAAGA AGAGCCAATA TCAACTATTA TTGGAAGAAG AACAATTATA 
AAAGCAAGTG GGATTGGAGA AGAAAGAGCG AGAATTGAAC GAGTTGCTGT GAATTATACG 
ATTCAAGGAT CTGCTAGCGA GATATTTAAA ACGGCTATTG TGGATATTGA ATCGAAAATT 
AAAGAATTCG GCGCACAAAT CGTCCTAACC ATCCACGATG AAGTTCTAGT CGAATGTCCA 
GAAATCCACG TGGCAGCAGC TTCCGAATCT ATTGAAAATT GCATGCAAAA TGCTCTTTCT 
CATTTATTAC GAGTTCCAAT GCGGGTTTCA ATGAAAACGG GACGATCATG GGCTGATTTA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498250.3
CDS	1..4986
Translation	MDEASCRFPEWVSSKIIDYYAEQNIKALFDWQIDVLNEARQFEDQHLIFSAPTSAGKSIVAELLSWKVASTGRKVLFVLPYISVAREKLHQIQRCWRRDDISVCGFIGPQASNPNEWLGAVCTIEKAASLTNRALSEDWFEEIGMIVVDEMHMVFDSSRGAHIEHMLSKVLLWNQSALEKVRIIGMSATIPELYRIGKWLDGAKVFEARFRPIVLQNHIVIGSELRKSGDNKVLREFSEDPLILLTEESFRRNSQTLVMISSKLDAEKTALNIASRFHEINKTDSSLLEILKERANGLLFIKHGLERNGCKDRNVMSTLAWGVAYHHAGLTMEERECIELGFREKNIVILVATSTLASGVNLPAERVLIKAQPRGPSALTSLNYRQMVGRAGRTGHATRGETYLLIKKCDRDAVLKIIETPIDQGVLTRKRDAERTNLSRFILEGICTGLTTTRSQIHDLCKLLLFNSENLQLSDIAIEMLLRNSFISQDENDDQLSPTQLGRAAIASSLPPEASLAIFEDLNSASRAIALDTELHMLYLVYFYKNSRAQIIQKIFKIYSIFILKKFKNLEPKFKKKISENITVHITNSIRKKQHFWHVTPINVSVWQECDWHHLFSIFSKLPSDHKRIAKLVGVSEKFILDQLQGRRNDKLLQIHIRFFSALALFDLISEMSIYEVSHKYRIPRGCLQTLQSQSATYAAMIVAFCLRLGWTYLKALLDGFATRLLFGVRSELSELVAIEGIDGQRARILHERGVTCLSHLSACDSSKLAHFLTLAVPYSSSNSNDGLGEWLFGEPRMRVDVAARTLKERARKVLIRRVQELGISVELPKFEENEENIQESCDSGLPDSCEGMEDELEEKENIVKMEEMTKSVTEMSLTDNTISFKSEDDLFKKEIKVEEDEVFIKKEIDEDEEEIVEETVIECLETSLLKLKASTDEVFLRRLSQTFSPIGRSRSILNNSLLEDSFDRPVPRSSIPILNFITPKRESPTPYFEDSFDRPIPGSLPISSSRRKSVLTNIANLDSSRRESINSNASDNNSFDVFVTPPTKSAKEEKRRIAVKHPRVGNIIYSPLTSSPVIKHPKLEINHFYLKDVCHDHNAWNLWTKSSTSTSSCSIRVSDDYTGIAIRTDAGNTFIPLLETFGGEPSPASKYFESFSKCIIPLNTRLEFLKTLAVTVEMYISSMEDAFLIFEKFGIKIFRLKVVRIAAYLNNVIDVEQEENSNFLPILMDRYSILDPEIRKTCSSSLHKAAVEVYSLKPIFEKMCCSGASLQLEMESCQTVLNIFYSGIVFDQALCNSFIYKIRKQIENLEENIWRLAYGKFNIHSSNEVANVLFYRLGLIYPETSGCKPKLRHLPTNKLILEQMNTQHPIVGKILEYRQIQHTLTQCLMPLAKFIGRIHCWFEMCTSTGRILTSVPNLQNVPKRISSDGMSARQLFIANSENLLIGADYKQLELRVLAHLSNDSNLVNLITSDRDLFEELSIQWNFPRDAVKQLCYGLIYGMGAKSLSELTRMSIEDAEKMLKAFFAMFPGVRSYINETKEKVCKEEPISTIIGRRTIIKASGIGEERARIERVAVNYTIQGSASEIFKTAIVDIESKIKEFGAQIVLTIHDEVLVECPEIHVAAASESIENCMQNALSHLLRVPMRVSMKTGRSWADLK

Target ORF information:

RefSeq Version	NM_065849.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA polymerase theta (polq-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065849.5

ORF Insert Sequence:

ATGGACGAGG CTTCCTGTAG ATTTCCAGAA TGGGTTTCTT CAAAAATCAT CGATTATTAC 
GCAGAGCAGA ACATCAAAGC GCTGTTCGAT TGGCAGATTG ATGTGTTGAA TGAGGCAAGA 
CAATTTGAAG ATCAACACTT GATATTCAGT GCGCCAACAA GTGCTGGGAA AAGCATTGTA 
GCTGAACTTT TATCATGGAA AGTCGCTTCA ACTGGTAGAA AGGTACTCTT CGTACTTCCT 
TATATCTCCG TGGCTCGAGA GAAACTTCAT CAAATTCAAC GGTGTTGGAG ACGAGATGAC 
ATTTCTGTCT GTGGCTTCAT TGGTCCACAG GCCTCAAATC CAAATGAGTG GCTTGGGGCA 
GTTTGTACCA TCGAAAAAGC AGCAAGTTTG ACAAATCGAG CATTATCAGA AGATTGGTTT 
GAGGAAATTG GTATGATAGT CGTTGATGAA ATGCACATGG TTTTCGATTC GTCTCGTGGT 
GCACACATTG AGCACATGCT CTCGAAAGTT TTGCTCTGGA ACCAATCTGC ACTGGAAAAA 
GTTAGAATTA TCGGAATGAG TGCAACTATT CCAGAACTTT ATCGAATAGG CAAATGGCTG 
GACGGTGCGA AAGTTTTCGA AGCAAGATTT CGACCAATTG TTCTACAAAA TCATATTGTA 
ATTGGTTCGG AGTTGAGGAA ATCTGGAGAT AACAAAGTTC TAAGAGAATT CTCTGAAGAT 
CCATTAATAT TGCTTACCGA AGAGAGTTTT CGCCGCAATT CTCAGACTTT GGTAATGATT 
TCTTCGAAAC TTGATGCCGA GAAGACTGCT TTGAATATTG CGTCGAGATT CCATGAGATC 
AATAAAACCG ACAGCTCATT GTTGGAAATA TTAAAAGAAC GAGCCAATGG ACTATTATTT 
ATAAAGCATG GATTGGAACG GAATGGTTGC AAGGATCGGA ATGTTATGTC AACACTTGCA 
TGGGGAGTAG CATATCATCA TGCTGGATTG ACGATGGAGG AAAGAGAATG CATTGAACTA 
GGATTTCGGG AGAAAAATAT TGTTATTCTT GTGGCTACAA GTACTTTGGC TTCAGGCGTA 
AATCTCCCTG CGGAACGAGT ACTCATCAAG GCTCAGCCAC GTGGCCCATC GGCTCTCACT 
TCTCTAAATT ACCGTCAGAT GGTGGGAAGA GCGGGAAGAA CAGGACATGC TACACGAGGT 
GAAACCTATC TTCTCATCAA GAAATGTGAT CGAGATGCCG TGCTGAAAAT CATCGAGACT 
CCGATTGATC AAGGTGTTCT AACAAGGAAA CGAGATGCTG AACGGACTAA TTTAAGCAGA 
TTTATATTAG AGGGAATCTG CACTGGGCTC ACGACAACAA GATCTCAGAT TCATGATCTC 
TGCAAGCTTC TCCTATTTAA TTCTGAAAAC CTTCAACTTT CTGACATTGC CATCGAGATG 
CTTCTTCGGA ATTCTTTCAT CTCCCAAGAC GAAAACGATG ATCAATTAAG TCCCACACAA 
CTCGGAAGAG CTGCAATTGC TTCATCTCTA CCACCTGAAG CATCACTTGC CATTTTTGAA 
GATTTGAATT CGGCGAGTCG AGCAATTGCA CTGGATACTG AGCTTCATAT GCTCTATTTG 
GTATATTTTT ACAAGAATTC GAGGGCCCAA ATAATTCAAA AAATATTCAA AATTTATTCA 
ATTTTCATTT TAAAAAAGTT TAAAAATTTA GAACCTAAAT TCAAAAAAAA AATTTCTGAA 
AACATCACCG TACATATTAC AAATTCTATA AGAAAAAAAC AACATTTTTG GCATGTAACC 
CCAATCAACG TAAGTGTTTG GCAGGAATGT GATTGGCATC ACCTTTTCTC AATTTTCTCG 
AAACTTCCAT CAGATCATAA ACGAATCGCA AAGTTGGTTG GAGTCAGTGA AAAATTTATT 
TTGGATCAAC TTCAAGGACG AAGAAATGAC AAATTGCTTC AAATTCATAT TCGATTCTTT 
TCTGCGTTGG CACTTTTTGA TTTGATCAGC GAAATGAGCA TTTATGAGGT ATCTCACAAG 
TACCGAATTC CTCGTGGATG TCTTCAAACA CTCCAATCAC AGAGCGCCAC TTACGCGGCA 
ATGATTGTGG CTTTTTGCCT TCGACTGGGA TGGACATATC TCAAGGCACT GCTTGATGGA 
TTTGCAACGA GATTGTTGTT CGGTGTTAGA TCAGAGCTCT CGGAGCTTGT GGCGATTGAA 
GGAATTGATG GACAGAGAGC CCGAATCCTA CATGAACGTG GCGTGACGTG TCTGTCACAT 
TTGTCCGCTT GCGATTCTTC GAAACTTGCA CACTTTCTAA CATTGGCTGT TCCATACTCA 
AGCTCGAATT CAAACGATGG ACTCGGCGAG TGGCTTTTTG GAGAGCCAAG AATGAGAGTT 
GATGTGGCGG CTAGAACATT GAAAGAACGG GCTCGAAAAG TATTGATCAG AAGAGTGCAG 
GAGCTGGGAA TTTCTGTGGA GTTGCCGAAA TTTGAGGAGA ACGAGGAAAA TATTCAAGAG 
AGTTGTGATA GTGGACTTCC GGATAGTTGT GAAGGAATGG AAGATGAGTT GGAGGAGAAA 
GAGAATATTG TGAAGATGGA AGAAATGACA AAGAGTGTCA CGGAAATGTC ACTTACAGAT 
AATACAATAT CATTTAAAAG TGAGGATGAT TTGTTCAAAA AAGAGATTAA AGTAGAAGAA 
GATGAAGTTT TTATAAAAAA AGAAATAGAC GAAGATGAAG AAGAAATAGT TGAAGAAACT 
GTAATCGAGT GTCTAGAAAC ATCTCTTCTG AAACTAAAAG CATCAACAGA TGAAGTATTC 
CTAAGAAGGC TTTCACAAAC ATTCTCACCT ATTGGAAGAA GTCGCAGTAT TCTCAATAAC 
TCATTACTTG AAGATTCATT TGATAGACCA GTACCCAGAA GCTCTATTCC AATTCTTAAT 
TTCATCACTC CAAAAAGGGA ATCGCCAACT CCATATTTCG AAGACTCATT CGATCGTCCA 
ATACCTGGAT CCTTACCAAT AAGTTCTTCA AGACGAAAGT CAGTTCTTAC AAATATTGCT 
AATCTGGATT CAAGTCGTCG TGAATCGATT AATTCCAATG CTTCGGATAA CAATTCATTC 
GATGTTTTTG TAACTCCACC AACAAAATCT GCAAAAGAAG AAAAACGAAG AATTGCTGTA 
AAACATCCAC GAGTTGGAAA TATTATATAT TCACCACTCA CTTCCAGTCC AGTCATAAAG 
CATCCAAAAC TTGAAATCAA TCATTTTTAT TTAAAAGATG TTTGCCATGA TCATAATGCT 
TGGAATTTAT GGACAAAATC AAGTACATCT ACTTCGAGTT GTTCAATTCG AGTTTCAGAT 
GATTATACTG GAATTGCAAT TAGAACCGAT GCGGGGAATA CTTTTATTCC ATTACTTGAA 
ACGTTTGGAG GAGAGCCATC ACCTGCATCC AAGTACTTCG AGTCATTCTC CAAATGCATC 
ATCCCTCTGA ACACTCGCTT GGAATTCCTG AAAACACTTG CGGTTACTGT AGAAATGTAT 
ATTTCGTCTA TGGAAGATGC TTTCCTGATT TTTGAAAAGT TTGGAATCAA GATTTTCCGA 
CTGAAAGTTG TTCGAATTGC TGCGTATCTG AATAATGTTA TCGACGTGGA GCAGGAGGAA 
AACTCGAATT TTCTGCCAAT TCTAATGGAT CGTTATTCAA TTTTAGATCC GGAAATTAGA 
AAAACTTGCT CTTCGAGCCT GCACAAAGCT GCCGTTGAAG TATATTCCCT GAAGCCGATC 
TTCGAGAAAA TGTGCTGTTC TGGAGCAAGT TTACAATTAG AAATGGAATC TTGTCAGACT 
GTCTTGAACA TTTTCTACTC TGGCATCGTA TTTGATCAAG CTTTATGCAA TTCTTTCATC 
TATAAAATTA GAAAACAAAT CGAGAATTTG GAAGAAAATA TTTGGAGACT TGCTTACGGA 
AAATTCAATA TTCATTCTTC AAATGAAGTT GCAAATGTTC TATTCTACCG CCTTGGCCTA 
ATTTATCCAG AAACTTCCGG ATGTAAACCA AAACTACGAC ATCTTCCAAC AAATAAGCTT 
ATATTGGAAC AAATGAATAC TCAACATCCG ATTGTTGGAA AAATTCTTGA ATATCGACAA 
ATTCAACATA CATTGACACA ATGCTTGATG CCACTTGCCA AGTTCATTGG AAGAATTCAC 
TGTTGGTTTG AAATGTGTAC ATCGACTGGA AGGATTTTGA CAAGTGTTCC GAATTTGCAA 
AACGTTCCAA AGCGAATATC GTCCGATGGA ATGTCTGCCC GTCAACTTTT CATCGCAAAC 
TCTGAAAATT TGTTAATCGG AGCCGATTAC AAGCAGCTGG AGCTTCGTGT TCTGGCACAT 
TTGAGCAATG ATTCGAATCT TGTCAATTTA ATCACATCGG ATAGAGATTT ATTTGAAGAA 
CTCTCAATTC AATGGAATTT CCCACGAGAT GCTGTTAAAC AGCTTTGCTA TGGTTTGATC 
TATGGAATGG GCGCCAAAAG TTTATCGGAG TTGACAAGAA TGAGCATAGA AGATGCTGAA 
AAGATGCTAA AGGCGTTTTT TGCAATGTTC CCTGGTGTAA GAAGCTATAT CAACGAAACA 
AAGGAAAAAG TTTGCAAAGA AGAGCCAATA TCAACTATTA TTGGAAGAAG AACAATTATA 
AAAGCAAGTG GGATTGGAGA AGAAAGAGCG AGAATTGAAC GAGTTGCTGT GAATTATACG 
ATTCAAGGAT CTGCTAGCGA GATATTTAAA ACGGCTATTG TGGATATTGA ATCGAAAATT 
AAAGAATTCG GCGCACAAAT CGTCCTAACC ATCCACGATG AAGTTCTAGT CGAATGTCCA 
GAAATCCACG TGGCAGCAGC TTCCGAATCT ATTGAAAATT GCATGCAAAA TGCTCTTTCT 
CATTTATTAC GAGTTCCAAT GCGGGTTTCA ATGAAAACGG GACGATCATG GGCTGATTTA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

polq-1 ( NM_065849.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498250.3 Caenorhabditis elegans DNA polymerase theta (polq-1), partial mRNA.

Price & Availability↑

CloneID	OCa341652
Clone ID Related Accession (Same CDS sequence)	NM_065849.4 , NM_065849.5
Accession Version	NM_065849.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	DNA polymerase theta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_065849.3. COMPLETENESS: incomplete on both ends.

ATGGACGAGG CTTCCTGTAG ATTTCCAGAA TGGGTTTCTT CAAAAATCAT CGATTATTAC 
GCAGAGCAGA ACATCAAAGC GCTGTTCGAT TGGCAGATTG ATGTGTTGAA TGAGGCAAGA 
CAATTTGAAG ATCAACACTT GATATTCAGT GCGCCAACAA GTGCTGGGAA AAGCATTGTA 
GCTGAACTTT TATCATGGAA AGTCGCTTCA ACTGGTAGAA AGGTACTCTT CGTACTTCCT 
TATATCTCCG TGGCTCGAGA GAAACTTCAT CAAATTCAAC GGTGTTGGAG ACGAGATGAC 
ATTTCTGTCT GTGGCTTCAT TGGTCCACAG GCCTCAAATC CAAATGAGTG GCTTGGGGCA 
GTTTGTACCA TCGAAAAAGC AGCAAGTTTG ACAAATCGAG CATTATCAGA AGATTGGTTT 
GAGGAAATTG GTATGATAGT CGTTGATGAA ATGCACATGG TTTTCGATTC GTCTCGTGGT 
GCACACATTG AGCACATGCT CTCGAAAGTT TTGCTCTGGA ACCAATCTGC ACTGGAAAAA 
GTTAGAATTA TCGGAATGAG TGCAACTATT CCAGAACTTT ATCGAATAGG CAAATGGCTG 
GACGGTGCGA AAGTTTTCGA AGCAAGATTT CGACCAATTG TTCTACAAAA TCATATTGTA 
ATTGGTTCGG AGTTGAGGAA ATCTGGAGAT AACAAAGTTC TAAGAGAATT CTCTGAAGAT 
CCATTAATAT TGCTTACCGA AGAGAGTTTT CGCCGCAATT CTCAGACTTT GGTAATGATT 
TCTTCGAAAC TTGATGCCGA GAAGACTGCT TTGAATATTG CGTCGAGATT CCATGAGATC 
AATAAAACCG ACAGCTCATT GTTGGAAATA TTAAAAGAAC GAGCCAATGG ACTATTATTT 
ATAAAGCATG GATTGGAACG GAATGGTTGC AAGGATCGGA ATGTTATGTC AACACTTGCA 
TGGGGAGTAG CATATCATCA TGCTGGATTG ACGATGGAGG AAAGAGAATG CATTGAACTA 
GGATTTCGGG AGAAAAATAT TGTTATTCTT GTGGCTACAA GTACTTTGGC TTCAGGCGTA 
AATCTCCCTG CGGAACGAGT ACTCATCAAG GCTCAGCCAC GTGGCCCATC GGCTCTCACT 
TCTCTAAATT ACCGTCAGAT GGTGGGAAGA GCGGGAAGAA CAGGACATGC TACACGAGGT 
GAAACCTATC TTCTCATCAA GAAATGTGAT CGAGATGCCG TGCTGAAAAT CATCGAGACT 
CCGATTGATC AAGGTGTTCT AACAAGGAAA CGAGATGCTG AACGGACTAA TTTAAGCAGA 
TTTATATTAG AGGGAATCTG CACTGGGCTC ACGACAACAA GATCTCAGAT TCATGATCTC 
TGCAAGCTTC TCCTATTTAA TTCTGAAAAC CTTCAACTTT CTGACATTGC CATCGAGATG 
CTTCTTCGGA ATTCTTTCAT CTCCCAAGAC GAAAACGATG ATCAATTAAG TCCCACACAA 
CTCGGAAGAG CTGCAATTGC TTCATCTCTA CCACCTGAAG CATCACTTGC CATTTTTGAA 
GATTTGAATT CGGCGAGTCG AGCAATTGCA CTGGATACTG AGCTTCATAT GCTCTATTTG 
GTATATTTTT ACAAGAATTC GAGGGCCCAA ATAATTCAAA AAATATTCAA AATTTATTCA 
ATTTTCATTT TAAAAAAGTT TAAAAATTTA GAACCTAAAT TCAAAAAAAA AATTTCTGAA 
AACATCACCG TACATATTAC AAATTCTATA AGAAAAAAAC AACATTTTTG GCATGTAACC 
CCAATCAACG TAAGTGTTTG GCAGGAATGT GATTGGCATC ACCTTTTCTC AATTTTCTCG 
AAACTTCCAT CAGATCATAA ACGAATCGCA AAGTTGGTTG GAGTCAGTGA AAAATTTATT 
TTGGATCAAC TTCAAGGACG AAGAAATGAC AAATTGCTTC AAATTCATAT TCGATTCTTT 
TCTGCGTTGG CACTTTTTGA TTTGATCAGC GAAATGAGCA TTTATGAGGT ATCTCACAAG 
TACCGAATTC CTCGTGGATG TCTTCAAACA CTCCAATCAC AGAGCGCCAC TTACGCGGCA 
ATGATTGTGG CTTTTTGCCT TCGACTGGGA TGGACATATC TCAAGGCACT GCTTGATGGA 
TTTGCAACGA GATTGTTGTT CGGTGTTAGA TCAGAGCTCT CGGAGCTTGT GGCGATTGAA 
GGAATTGATG GACAGAGAGC CCGAATCCTA CATGAACGTG GCGTGACGTG TCTGTCACAT 
TTGTCCGCTT GCGATTCTTC GAAACTTGCA CACTTTCTAA CATTGGCTGT TCCATACTCA 
AGCTCGAATT CAAACGATGG ACTCGGCGAG TGGCTTTTTG GAGAGCCAAG AATGAGAGTT 
GATGTGGCGG CTAGAACATT GAAAGAACGG GCTCGAAAAG TATTGATCAG AAGAGTGCAG 
GAGCTGGGAA TTTCTGTGGA GTTGCCGAAA TTTGAGGAGA ACGAGGAAAA TATTCAAGAG 
AGTTGTGATA GTGGACTTCC GGATAGTTGT GAAGGAATGG AAGATGAGTT GGAGGAGAAA 
GAGAATATTG TGAAGATGGA AGAAATGACA AAGAGTGTCA CGGAAATGTC ACTTACAGAT 
AATACAATAT CATTTAAAAG TGAGGATGAT TTGTTCAAAA AAGAGATTAA AGTAGAAGAA 
GATGAAGTTT TTATAAAAAA AGAAATAGAC GAAGATGAAG AAGAAATAGT TGAAGAAACT 
GTAATCGAGT GTCTAGAAAC ATCTCTTCTG AAACTAAAAG CATCAACAGA TGAAGTATTC 
CTAAGAAGGC TTTCACAAAC ATTCTCACCT ATTGGAAGAA GTCGCAGTAT TCTCAATAAC 
TCATTACTTG AAGATTCATT TGATAGACCA GTACCCAGAA GCTCTATTCC AATTCTTAAT 
TTCATCACTC CAAAAAGGGA ATCGCCAACT CCATATTTCG AAGACTCATT CGATCGTCCA 
ATACCTGGAT CCTTACCAAT AAGTTCTTCA AGACGAAAGT CAGTTCTTAC AAATATTGCT 
AATCTGGATT CAAGTCGTCG TGAATCGATT AATTCCAATG CTTCGGATAA CAATTCATTC 
GATGTTTTTG TAACTCCACC AACAAAATCT GCAAAAGAAG AAAAACGAAG AATTGCTGTA 
AAACATCCAC GAGTTGGAAA TATTATATAT TCACCACTCA CTTCCAGTCC AGTCATAAAG 
CATCCAAAAC TTGAAATCAA TCATTTTTAT TTAAAAGATG TTTGCCATGA TCATAATGCT 
TGGAATTTAT GGACAAAATC AAGTACATCT ACTTCGAGTT GTTCAATTCG AGTTTCAGAT 
GATTATACTG GAATTGCAAT TAGAACCGAT GCGGGGAATA CTTTTATTCC ATTACTTGAA 
ACGTTTGGAG GAGAGCCATC ACCTGCATCC AAGTACTTCG AGTCATTCTC CAAATGCATC 
ATCCCTCTGA ACACTCGCTT GGAATTCCTG AAAACACTTG CGGTTACTGT AGAAATGTAT 
ATTTCGTCTA TGGAAGATGC TTTCCTGATT TTTGAAAAGT TTGGAATCAA GATTTTCCGA 
CTGAAAGTTG TTCGAATTGC TGCGTATCTG AATAATGTTA TCGACGTGGA GCAGGAGGAA 
AACTCGAATT TTCTGCCAAT TCTAATGGAT CGTTATTCAA TTTTAGATCC GGAAATTAGA 
AAAACTTGCT CTTCGAGCCT GCACAAAGCT GCCGTTGAAG TATATTCCCT GAAGCCGATC 
TTCGAGAAAA TGTGCTGTTC TGGAGCAAGT TTACAATTAG AAATGGAATC TTGTCAGACT 
GTCTTGAACA TTTTCTACTC TGGCATCGTA TTTGATCAAG CTTTATGCAA TTCTTTCATC 
TATAAAATTA GAAAACAAAT CGAGAATTTG GAAGAAAATA TTTGGAGACT TGCTTACGGA 
AAATTCAATA TTCATTCTTC AAATGAAGTT GCAAATGTTC TATTCTACCG CCTTGGCCTA 
ATTTATCCAG AAACTTCCGG ATGTAAACCA AAACTACGAC ATCTTCCAAC AAATAAGCTT 
ATATTGGAAC AAATGAATAC TCAACATCCG ATTGTTGGAA AAATTCTTGA ATATCGACAA 
ATTCAACATA CATTGACACA ATGCTTGATG CCACTTGCCA AGTTCATTGG AAGAATTCAC 
TGTTGGTTTG AAATGTGTAC ATCGACTGGA AGGATTTTGA CAAGTGTTCC GAATTTGCAA 
AACGTTCCAA AGCGAATATC GTCCGATGGA ATGTCTGCCC GTCAACTTTT CATCGCAAAC 
TCTGAAAATT TGTTAATCGG AGCCGATTAC AAGCAGCTGG AGCTTCGTGT TCTGGCACAT 
TTGAGCAATG ATTCGAATCT TGTCAATTTA ATCACATCGG ATAGAGATTT ATTTGAAGAA 
CTCTCAATTC AATGGAATTT CCCACGAGAT GCTGTTAAAC AGCTTTGCTA TGGTTTGATC 
TATGGAATGG GCGCCAAAAG TTTATCGGAG TTGACAAGAA TGAGCATAGA AGATGCTGAA 
AAGATGCTAA AGGCGTTTTT TGCAATGTTC CCTGGTGTAA GAAGCTATAT CAACGAAACA 
AAGGAAAAAG TTTGCAAAGA AGAGCCAATA TCAACTATTA TTGGAAGAAG AACAATTATA 
AAAGCAAGTG GGATTGGAGA AGAAAGAGCG AGAATTGAAC GAGTTGCTGT GAATTATACG 
ATTCAAGGAT CTGCTAGCGA GATATTTAAA ACGGCTATTG TGGATATTGA ATCGAAAATT 
AAAGAATTCG GCGCACAAAT CGTCCTAACC ATCCACGATG AAGTTCTAGT CGAATGTCCA 
GAAATCCACG TGGCAGCAGC TTCCGAATCT ATTGAAAATT GCATGCAAAA TGCTCTTTCT 
CATTTATTAC GAGTTCCAAT GCGGGTTTCA ATGAAAACGG GACGATCATG GGCTGATTTA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498250.3
CDS	1..4986
Translation	MDEASCRFPEWVSSKIIDYYAEQNIKALFDWQIDVLNEARQFEDQHLIFSAPTSAGKSIVAELLSWKVASTGRKVLFVLPYISVAREKLHQIQRCWRRDDISVCGFIGPQASNPNEWLGAVCTIEKAASLTNRALSEDWFEEIGMIVVDEMHMVFDSSRGAHIEHMLSKVLLWNQSALEKVRIIGMSATIPELYRIGKWLDGAKVFEARFRPIVLQNHIVIGSELRKSGDNKVLREFSEDPLILLTEESFRRNSQTLVMISSKLDAEKTALNIASRFHEINKTDSSLLEILKERANGLLFIKHGLERNGCKDRNVMSTLAWGVAYHHAGLTMEERECIELGFREKNIVILVATSTLASGVNLPAERVLIKAQPRGPSALTSLNYRQMVGRAGRTGHATRGETYLLIKKCDRDAVLKIIETPIDQGVLTRKRDAERTNLSRFILEGICTGLTTTRSQIHDLCKLLLFNSENLQLSDIAIEMLLRNSFISQDENDDQLSPTQLGRAAIASSLPPEASLAIFEDLNSASRAIALDTELHMLYLVYFYKNSRAQIIQKIFKIYSIFILKKFKNLEPKFKKKISENITVHITNSIRKKQHFWHVTPINVSVWQECDWHHLFSIFSKLPSDHKRIAKLVGVSEKFILDQLQGRRNDKLLQIHIRFFSALALFDLISEMSIYEVSHKYRIPRGCLQTLQSQSATYAAMIVAFCLRLGWTYLKALLDGFATRLLFGVRSELSELVAIEGIDGQRARILHERGVTCLSHLSACDSSKLAHFLTLAVPYSSSNSNDGLGEWLFGEPRMRVDVAARTLKERARKVLIRRVQELGISVELPKFEENEENIQESCDSGLPDSCEGMEDELEEKENIVKMEEMTKSVTEMSLTDNTISFKSEDDLFKKEIKVEEDEVFIKKEIDEDEEEIVEETVIECLETSLLKLKASTDEVFLRRLSQTFSPIGRSRSILNNSLLEDSFDRPVPRSSIPILNFITPKRESPTPYFEDSFDRPIPGSLPISSSRRKSVLTNIANLDSSRRESINSNASDNNSFDVFVTPPTKSAKEEKRRIAVKHPRVGNIIYSPLTSSPVIKHPKLEINHFYLKDVCHDHNAWNLWTKSSTSTSSCSIRVSDDYTGIAIRTDAGNTFIPLLETFGGEPSPASKYFESFSKCIIPLNTRLEFLKTLAVTVEMYISSMEDAFLIFEKFGIKIFRLKVVRIAAYLNNVIDVEQEENSNFLPILMDRYSILDPEIRKTCSSSLHKAAVEVYSLKPIFEKMCCSGASLQLEMESCQTVLNIFYSGIVFDQALCNSFIYKIRKQIENLEENIWRLAYGKFNIHSSNEVANVLFYRLGLIYPETSGCKPKLRHLPTNKLILEQMNTQHPIVGKILEYRQIQHTLTQCLMPLAKFIGRIHCWFEMCTSTGRILTSVPNLQNVPKRISSDGMSARQLFIANSENLLIGADYKQLELRVLAHLSNDSNLVNLITSDRDLFEELSIQWNFPRDAVKQLCYGLIYGMGAKSLSELTRMSIEDAEKMLKAFFAMFPGVRSYINETKEKVCKEEPISTIIGRRTIIKASGIGEERARIERVAVNYTIQGSASEIFKTAIVDIESKIKEFGAQIVLTIHDEVLVECPEIHVAAASESIENCMQNALSHLLRVPMRVSMKTGRSWADLK

Target ORF information:

RefSeq Version	NM_065849.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA polymerase theta (polq-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065849.4

ORF Insert Sequence:

ATGGACGAGG CTTCCTGTAG ATTTCCAGAA TGGGTTTCTT CAAAAATCAT CGATTATTAC 
GCAGAGCAGA ACATCAAAGC GCTGTTCGAT TGGCAGATTG ATGTGTTGAA TGAGGCAAGA 
CAATTTGAAG ATCAACACTT GATATTCAGT GCGCCAACAA GTGCTGGGAA AAGCATTGTA 
GCTGAACTTT TATCATGGAA AGTCGCTTCA ACTGGTAGAA AGGTACTCTT CGTACTTCCT 
TATATCTCCG TGGCTCGAGA GAAACTTCAT CAAATTCAAC GGTGTTGGAG ACGAGATGAC 
ATTTCTGTCT GTGGCTTCAT TGGTCCACAG GCCTCAAATC CAAATGAGTG GCTTGGGGCA 
GTTTGTACCA TCGAAAAAGC AGCAAGTTTG ACAAATCGAG CATTATCAGA AGATTGGTTT 
GAGGAAATTG GTATGATAGT CGTTGATGAA ATGCACATGG TTTTCGATTC GTCTCGTGGT 
GCACACATTG AGCACATGCT CTCGAAAGTT TTGCTCTGGA ACCAATCTGC ACTGGAAAAA 
GTTAGAATTA TCGGAATGAG TGCAACTATT CCAGAACTTT ATCGAATAGG CAAATGGCTG 
GACGGTGCGA AAGTTTTCGA AGCAAGATTT CGACCAATTG TTCTACAAAA TCATATTGTA 
ATTGGTTCGG AGTTGAGGAA ATCTGGAGAT AACAAAGTTC TAAGAGAATT CTCTGAAGAT 
CCATTAATAT TGCTTACCGA AGAGAGTTTT CGCCGCAATT CTCAGACTTT GGTAATGATT 
TCTTCGAAAC TTGATGCCGA GAAGACTGCT TTGAATATTG CGTCGAGATT CCATGAGATC 
AATAAAACCG ACAGCTCATT GTTGGAAATA TTAAAAGAAC GAGCCAATGG ACTATTATTT 
ATAAAGCATG GATTGGAACG GAATGGTTGC AAGGATCGGA ATGTTATGTC AACACTTGCA 
TGGGGAGTAG CATATCATCA TGCTGGATTG ACGATGGAGG AAAGAGAATG CATTGAACTA 
GGATTTCGGG AGAAAAATAT TGTTATTCTT GTGGCTACAA GTACTTTGGC TTCAGGCGTA 
AATCTCCCTG CGGAACGAGT ACTCATCAAG GCTCAGCCAC GTGGCCCATC GGCTCTCACT 
TCTCTAAATT ACCGTCAGAT GGTGGGAAGA GCGGGAAGAA CAGGACATGC TACACGAGGT 
GAAACCTATC TTCTCATCAA GAAATGTGAT CGAGATGCCG TGCTGAAAAT CATCGAGACT 
CCGATTGATC AAGGTGTTCT AACAAGGAAA CGAGATGCTG AACGGACTAA TTTAAGCAGA 
TTTATATTAG AGGGAATCTG CACTGGGCTC ACGACAACAA GATCTCAGAT TCATGATCTC 
TGCAAGCTTC TCCTATTTAA TTCTGAAAAC CTTCAACTTT CTGACATTGC CATCGAGATG 
CTTCTTCGGA ATTCTTTCAT CTCCCAAGAC GAAAACGATG ATCAATTAAG TCCCACACAA 
CTCGGAAGAG CTGCAATTGC TTCATCTCTA CCACCTGAAG CATCACTTGC CATTTTTGAA 
GATTTGAATT CGGCGAGTCG AGCAATTGCA CTGGATACTG AGCTTCATAT GCTCTATTTG 
GTATATTTTT ACAAGAATTC GAGGGCCCAA ATAATTCAAA AAATATTCAA AATTTATTCA 
ATTTTCATTT TAAAAAAGTT TAAAAATTTA GAACCTAAAT TCAAAAAAAA AATTTCTGAA 
AACATCACCG TACATATTAC AAATTCTATA AGAAAAAAAC AACATTTTTG GCATGTAACC 
CCAATCAACG TAAGTGTTTG GCAGGAATGT GATTGGCATC ACCTTTTCTC AATTTTCTCG 
AAACTTCCAT CAGATCATAA ACGAATCGCA AAGTTGGTTG GAGTCAGTGA AAAATTTATT 
TTGGATCAAC TTCAAGGACG AAGAAATGAC AAATTGCTTC AAATTCATAT TCGATTCTTT 
TCTGCGTTGG CACTTTTTGA TTTGATCAGC GAAATGAGCA TTTATGAGGT ATCTCACAAG 
TACCGAATTC CTCGTGGATG TCTTCAAACA CTCCAATCAC AGAGCGCCAC TTACGCGGCA 
ATGATTGTGG CTTTTTGCCT TCGACTGGGA TGGACATATC TCAAGGCACT GCTTGATGGA 
TTTGCAACGA GATTGTTGTT CGGTGTTAGA TCAGAGCTCT CGGAGCTTGT GGCGATTGAA 
GGAATTGATG GACAGAGAGC CCGAATCCTA CATGAACGTG GCGTGACGTG TCTGTCACAT 
TTGTCCGCTT GCGATTCTTC GAAACTTGCA CACTTTCTAA CATTGGCTGT TCCATACTCA 
AGCTCGAATT CAAACGATGG ACTCGGCGAG TGGCTTTTTG GAGAGCCAAG AATGAGAGTT 
GATGTGGCGG CTAGAACATT GAAAGAACGG GCTCGAAAAG TATTGATCAG AAGAGTGCAG 
GAGCTGGGAA TTTCTGTGGA GTTGCCGAAA TTTGAGGAGA ACGAGGAAAA TATTCAAGAG 
AGTTGTGATA GTGGACTTCC GGATAGTTGT GAAGGAATGG AAGATGAGTT GGAGGAGAAA 
GAGAATATTG TGAAGATGGA AGAAATGACA AAGAGTGTCA CGGAAATGTC ACTTACAGAT 
AATACAATAT CATTTAAAAG TGAGGATGAT TTGTTCAAAA AAGAGATTAA AGTAGAAGAA 
GATGAAGTTT TTATAAAAAA AGAAATAGAC GAAGATGAAG AAGAAATAGT TGAAGAAACT 
GTAATCGAGT GTCTAGAAAC ATCTCTTCTG AAACTAAAAG CATCAACAGA TGAAGTATTC 
CTAAGAAGGC TTTCACAAAC ATTCTCACCT ATTGGAAGAA GTCGCAGTAT TCTCAATAAC 
TCATTACTTG AAGATTCATT TGATAGACCA GTACCCAGAA GCTCTATTCC AATTCTTAAT 
TTCATCACTC CAAAAAGGGA ATCGCCAACT CCATATTTCG AAGACTCATT CGATCGTCCA 
ATACCTGGAT CCTTACCAAT AAGTTCTTCA AGACGAAAGT CAGTTCTTAC AAATATTGCT 
AATCTGGATT CAAGTCGTCG TGAATCGATT AATTCCAATG CTTCGGATAA CAATTCATTC 
GATGTTTTTG TAACTCCACC AACAAAATCT GCAAAAGAAG AAAAACGAAG AATTGCTGTA 
AAACATCCAC GAGTTGGAAA TATTATATAT TCACCACTCA CTTCCAGTCC AGTCATAAAG 
CATCCAAAAC TTGAAATCAA TCATTTTTAT TTAAAAGATG TTTGCCATGA TCATAATGCT 
TGGAATTTAT GGACAAAATC AAGTACATCT ACTTCGAGTT GTTCAATTCG AGTTTCAGAT 
GATTATACTG GAATTGCAAT TAGAACCGAT GCGGGGAATA CTTTTATTCC ATTACTTGAA 
ACGTTTGGAG GAGAGCCATC ACCTGCATCC AAGTACTTCG AGTCATTCTC CAAATGCATC 
ATCCCTCTGA ACACTCGCTT GGAATTCCTG AAAACACTTG CGGTTACTGT AGAAATGTAT 
ATTTCGTCTA TGGAAGATGC TTTCCTGATT TTTGAAAAGT TTGGAATCAA GATTTTCCGA 
CTGAAAGTTG TTCGAATTGC TGCGTATCTG AATAATGTTA TCGACGTGGA GCAGGAGGAA 
AACTCGAATT TTCTGCCAAT TCTAATGGAT CGTTATTCAA TTTTAGATCC GGAAATTAGA 
AAAACTTGCT CTTCGAGCCT GCACAAAGCT GCCGTTGAAG TATATTCCCT GAAGCCGATC 
TTCGAGAAAA TGTGCTGTTC TGGAGCAAGT TTACAATTAG AAATGGAATC TTGTCAGACT 
GTCTTGAACA TTTTCTACTC TGGCATCGTA TTTGATCAAG CTTTATGCAA TTCTTTCATC 
TATAAAATTA GAAAACAAAT CGAGAATTTG GAAGAAAATA TTTGGAGACT TGCTTACGGA 
AAATTCAATA TTCATTCTTC AAATGAAGTT GCAAATGTTC TATTCTACCG CCTTGGCCTA 
ATTTATCCAG AAACTTCCGG ATGTAAACCA AAACTACGAC ATCTTCCAAC AAATAAGCTT 
ATATTGGAAC AAATGAATAC TCAACATCCG ATTGTTGGAA AAATTCTTGA ATATCGACAA 
ATTCAACATA CATTGACACA ATGCTTGATG CCACTTGCCA AGTTCATTGG AAGAATTCAC 
TGTTGGTTTG AAATGTGTAC ATCGACTGGA AGGATTTTGA CAAGTGTTCC GAATTTGCAA 
AACGTTCCAA AGCGAATATC GTCCGATGGA ATGTCTGCCC GTCAACTTTT CATCGCAAAC 
TCTGAAAATT TGTTAATCGG AGCCGATTAC AAGCAGCTGG AGCTTCGTGT TCTGGCACAT 
TTGAGCAATG ATTCGAATCT TGTCAATTTA ATCACATCGG ATAGAGATTT ATTTGAAGAA 
CTCTCAATTC AATGGAATTT CCCACGAGAT GCTGTTAAAC AGCTTTGCTA TGGTTTGATC 
TATGGAATGG GCGCCAAAAG TTTATCGGAG TTGACAAGAA TGAGCATAGA AGATGCTGAA 
AAGATGCTAA AGGCGTTTTT TGCAATGTTC CCTGGTGTAA GAAGCTATAT CAACGAAACA 
AAGGAAAAAG TTTGCAAAGA AGAGCCAATA TCAACTATTA TTGGAAGAAG AACAATTATA 
AAAGCAAGTG GGATTGGAGA AGAAAGAGCG AGAATTGAAC GAGTTGCTGT GAATTATACG 
ATTCAAGGAT CTGCTAGCGA GATATTTAAA ACGGCTATTG TGGATATTGA ATCGAAAATT 
AAAGAATTCG GCGCACAAAT CGTCCTAACC ATCCACGATG AAGTTCTAGT CGAATGTCCA 
GAAATCCACG TGGCAGCAGC TTCCGAATCT ATTGAAAATT GCATGCAAAA TGCTCTTTCT 
CATTTATTAC GAGTTCCAAT GCGGGTTTCA ATGAAAACGG GACGATCATG GGCTGATTTA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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polq-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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