hrde-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	hrde-1
Entrez Gene ID	175535
Full Name	Heritable RNAi Deficient
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_065433.4	NP_497834.1	Heritable RNAi Deficient
NM_065433.5	NP_497834.1	Heritable RNAi Deficient

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	wago-9	NP_497834.1
Caenorhabditis elegans (roundworm)	wago-11	NP_494593.1
Caenorhabditis elegans (roundworm)	nrde-3	NP_508092.1
Caenorhabditis elegans (roundworm)	C14B1.7	NP_001254877.1
Caenorhabditis elegans (roundworm)	wago-10	NP_503177.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HRDE-1 physically interacts with the helicase EMB-4.
Title: The Helicase Aquarius/EMB-4 Is Required to Overcome Intronic Barriers to Allow Nuclear RNAi Pathways to Heritably Silence Transcription.

Nuclear import of the secondary siRNAs by the Argonaute HRDE-1 leads to the production of a distinct class of small RNAs that map throughout the transcript, which we term tertiary siRNAs.
Title: Tertiary siRNAs mediate paramutation in C. elegans.

results establish that the Argonaute protein HRDE-1 directs gene-silencing events in germ-cell nuclei that drive multigenerational RNAi inheritance and promote immortality of the germ-cell lineage
Title: A nuclear Argonaute promotes multigenerational epigenetic inheritance and germline immortality.

The following hrde-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hrde-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa341988	NM_065433.5 Latest version!	Caenorhabditis elegans Heritable RNAi Deficient (hrde-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa341988	NM_065433.4	Caenorhabditis elegans Heritable RNAi Deficient (hrde-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hrde-1 ( NM_065433.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_497834.1 Caenorhabditis elegans Heritable RNAi Deficient (hrde-1), partial mRNA.

Price & Availability↑

CloneID	OCa341988
Clone ID Related Accession (Same CDS sequence)	NM_065433.4 , NM_065433.5
Accession Version	NM_065433.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Heritable RNAi Deficient
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_065433.3. COMPLETENESS: incomplete on both ends.

ATGGCCGACT TGCTCGACAA AATTATGGGA AGTTCGTCTT CCAACAGGAT TCCCAAACGA 
GACAACCGAA TGAATCAGGA CAAGGACGAA CCGACGTCAA AGAGAAGTGC ACCAATGTTC 
TCTACTCCGA AGTCAACAAC ACCAATTGGA CGGGACTCTT TCGCAACGAT GGACACGATG 
AATGTTCAGA TGAACATGTT TCCAGTGGAC ATCAAGGATA TGCCCCACAA GATCCAACGC 
TTTCAAGTTG ACGTCATCGT CTGTTCCAGC AACGGAAAAC AGATCAATGC CAACCTTGGA 
GTTTTGGCGG CCAAGGGAGA TGTCAATTCC CATAACCGTC GCTTGGCACA ATACTACATC 
ATGAGAACAG TACATGACAA GCTTCTGGTG AAGTTCAGTG GAAAGAGTCA CCACTTCCTT 
GCTTACGACT GCGCTGCGAC TCTCTATCTT CCGGAGGGAG TCTACACTGG TGACGATCAA 
GAAGAAGTCA CGTTGACGAT CGATGACTTC CCGAAGGAAG AATGGAAATT CGTGTCGAAG 
TTGTCTCGCA GAAAGGACGA TTCTTACCTT GTTGTTCTCA AACCTGCCGG ATTTGTGTAC 
ACCCAAGGAG AAGTTGCCCA GGAGGAGGCT AATCGTATGG AGTTGACTCG TATTATCGAA 
ATCGTCACTT CACAAAAGCT CAACAATGAG GATTACTTAC AATTTGGTAA TGCCACATTC 
CCACGTCTTT CTCCGCCACA CTCGGAACCT GATGCTATTT CGGAGATCCG CTCAGGTTTT 
GCGAAAGTAT CTCGTCTATC GCAAAACGGA GGTGGCAAAG CTTTCATGAC GGTTGATACG 
AAGATTTCTC CATTTTACAA GGATACATCA GTTATCAAGT TTTCAAGCAA CAAGTTATCC 
GAAATGAAAG GTGGAGGCGG TGGAAGAGGC GGATACGGAA GAAGTGACTC GCGAGACTCT 
CGTGGTGGAT ACCGTGGCGG CCGTTCCGAC TCGAGAGACT TTCGTGGTGT CTATGGAAAC 
CGAGGTGGCA ACGACAGGTA CCGAGATGAA AGCAGAGGAC GTCGCGACAT GTACGATTCC 
CGTCGAGACA GTGGTTCTTC AAATGGAGCG GACTACTCTC CGAGTGATGC TGCTGAGTTG 
GAACACGCTT TTGGCGAAAG AGGAAACACG AAGCGATGCA TTGAAGAAGC ACTCAAAGGA 
TTGGACGTGG AATGCACTCA CTTGAAGGGT AACCTTATTC GCGTATCCTC GATTGCCGAG 
AACAATGCTG AGAACACTTC GTTCATGATG AAGGACGACA AGGGAGAACG CGAGGTCACC 
GTGGCGGAAT ATTTCCTGTT GCAGTACAAC ATCAAGCTGA AATATCCGAG ACTTCCTCTC 
GTCGTCTCAA AAAGATTCAA ACATGAATCT TTCTTCCCAA TGGAGTTGCT TCGAATTGCT 
CCAGGCCAGC GGATCAAGGT CAACAAGATG AGCCCGACAG TACAATCGGC GATGACAGGA 
AGAAATGCGT CTATGCCACA GCATCATGTG AAGTTGGTTC AAGATATTCT CCGCGACAAC 
TTGAAGCTTG AACAAAATAA GTACATGGAT GCTTTCGGAA TCAAACTGAT GTCGACTGAG 
CCAATCCAAA TGACTGCGAA GCTCCTCCCA CCAGCTCAAA TCAAGTTCAA GGGCCAGACT 
TACATGCCTG ACATGAGCCG TCCAGCGTTC CGCACACAGG ACAAGTTTGT CGAACCAGCA 
CGGATCAGAA AAATTGGTAT TGTTGTCTTT GACAACTGTA TCCAAATGAG ACAGGCCGAA 
GATTTCTGCG ACAAGCTGTC CAATTTCTGT CGTGACAATG GAATCACGGT CGAAAAGGAC 
TCGCGAGATT GGTCCATCCG TGAATTGAAC TCCAGTGATT CCGTTGCTAT CCAAAATCTG 
ATGAAGAAAT GGCTCGATGA TAGAGTGGAC ATTCTTGTTG GAATAGCAAG GGAGAAGAAG 
CCGGATGTTC ACGACATACT CAAATACTTT GAGGAATCGA TTGGCCTGCA AACAATCCAA 
TTGTGTCAAC AGACTGTCGA TAAAATGATG GGAGGTCAAG GTGGACGCCA AACGATTGAC 
AACGTCATGA GAAAGTTCAA CCTCAAGTGT GGTGGAACCA ACTTCTTCGT CGAGATCCCG 
AATGCTGTGC GTGGAAAAGC AGTGTGCTCA AACAATGAAA CATTGAGAAA GAAACTTCTC 
GAACATGTAC AATTCATCGG ATTCGAGATT TCTCATGGCG CATCACGCAC TTTGTTCGAT 
CGCAGTAGAT CACAAATGGA TGGTGAGCCA TCAGTTGTTG GAGTCTCGTA TTCCCTGACC 
AACTCGACGC AGCTCGGAGG CTTCACATAC CTTCAAACCC AAAAGGAGTA CAAACTTCAA 
AAACTTGATG AATTCTTCCC AAAGTGTGTC AGATCGTACA AGGAGCATTC GAAGACACTC 
CCAACAAGAA TCGTCATCTA TCGTGTTGGT GCTGGAGAAG GAAACTTCAA TCGTGTTAAG 
GAAGAAGTCG AAGAGATGCG ACGAACATTT GACAAGATTC AGCCCGGATA CCGTCCACAT 
CTCGTGGTGA TCATCGCACA AAGAGCTTCA CATGCTCGCG TTTTTCCATC ATGTATCAGT 
GGCAACAGAG CGACGGATCA GAACATCCCA TCTGGAACAT GTGTCGAGAA TGTCCTGACA 
TCCTACGGAT ACGACGAGTT CATTTTGTCT TCACAGACGC CGCTTATTGG TACCGTTCGT 
CCGTGCAAGT ACACGATCCT TGTCAATGAT GCCAAATGGT CTAAAAACGA GCTCATGCAC 
TTGACGTACT TCCGCGCATT CGGTCATCAA GTGTCCTACC AGCCACCATC GGTTCCAGAT 
GTTCTTTATG CGGCAGAAAA TCTGGCTAAA CGAGGACGCA ACAATTACAA AATTCATCAG 
CGTTACGTGA ATTTGCAAGC AGTTGAGAAC CGCATCATCA AAGACCATTC GGAACTCATC 
AACGAAGACA TGCGAGAAGA ACTGGCGGCA GCCATTGTTG ATGAGATGTC CGTTGCAATG 
AACGGAATGA CCATTCCGAA GCGAAACTTC TGGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_497834.1
CDS	1..3099
Translation	MADLLDKIMGSSSSNRIPKRDNRMNQDKDEPTSKRSAPMFSTPKSTTPIGRDSFATMDTMNVQMNMFPVDIKDMPHKIQRFQVDVIVCSSNGKQINANLGVLAAKGDVNSHNRRLAQYYIMRTVHDKLLVKFSGKSHHFLAYDCAATLYLPEGVYTGDDQEEVTLTIDDFPKEEWKFVSKLSRRKDDSYLVVLKPAGFVYTQGEVAQEEANRMELTRIIEIVTSQKLNNEDYLQFGNATFPRLSPPHSEPDAISEIRSGFAKVSRLSQNGGGKAFMTVDTKISPFYKDTSVIKFSSNKLSEMKGGGGGRGGYGRSDSRDSRGGYRGGRSDSRDFRGVYGNRGGNDRYRDESRGRRDMYDSRRDSGSSNGADYSPSDAAELEHAFGERGNTKRCIEEALKGLDVECTHLKGNLIRVSSIAENNAENTSFMMKDDKGEREVTVAEYFLLQYNIKLKYPRLPLVVSKRFKHESFFPMELLRIAPGQRIKVNKMSPTVQSAMTGRNASMPQHHVKLVQDILRDNLKLEQNKYMDAFGIKLMSTEPIQMTAKLLPPAQIKFKGQTYMPDMSRPAFRTQDKFVEPARIRKIGIVVFDNCIQMRQAEDFCDKLSNFCRDNGITVEKDSRDWSIRELNSSDSVAIQNLMKKWLDDRVDILVGIAREKKPDVHDILKYFEESIGLQTIQLCQQTVDKMMGGQGGRQTIDNVMRKFNLKCGGTNFFVEIPNAVRGKAVCSNNETLRKKLLEHVQFIGFEISHGASRTLFDRSRSQMDGEPSVVGVSYSLTNSTQLGGFTYLQTQKEYKLQKLDEFFPKCVRSYKEHSKTLPTRIVIYRVGAGEGNFNRVKEEVEEMRRTFDKIQPGYRPHLVVIIAQRASHARVFPSCISGNRATDQNIPSGTCVENVLTSYGYDEFILSSQTPLIGTVRPCKYTILVNDAKWSKNELMHLTYFRAFGHQVSYQPPSVPDVLYAAENLAKRGRNNYKIHQRYVNLQAVENRIIKDHSELINEDMREELAAAIVDEMSVAMNGMTIPKRNFWA

Target ORF information:

RefSeq Version	NM_065433.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Heritable RNAi Deficient (hrde-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065433.4

ORF Insert Sequence:

ATGGCCGACT TGCTCGACAA AATTATGGGA AGTTCGTCTT CCAACAGGAT TCCCAAACGA 
GACAACCGAA TGAATCAGGA CAAGGACGAA CCGACGTCAA AGAGAAGTGC ACCAATGTTC 
TCTACTCCGA AGTCAACAAC ACCAATTGGA CGGGACTCTT TCGCAACGAT GGACACGATG 
AATGTTCAGA TGAACATGTT TCCAGTGGAC ATCAAGGATA TGCCCCACAA GATCCAACGC 
TTTCAAGTTG ACGTCATCGT CTGTTCCAGC AACGGAAAAC AGATCAATGC CAACCTTGGA 
GTTTTGGCGG CCAAGGGAGA TGTCAATTCC CATAACCGTC GCTTGGCACA ATACTACATC 
ATGAGAACAG TACATGACAA GCTTCTGGTG AAGTTCAGTG GAAAGAGTCA CCACTTCCTT 
GCTTACGACT GCGCTGCGAC TCTCTATCTT CCGGAGGGAG TCTACACTGG TGACGATCAA 
GAAGAAGTCA CGTTGACGAT CGATGACTTC CCGAAGGAAG AATGGAAATT CGTGTCGAAG 
TTGTCTCGCA GAAAGGACGA TTCTTACCTT GTTGTTCTCA AACCTGCCGG ATTTGTGTAC 
ACCCAAGGAG AAGTTGCCCA GGAGGAGGCT AATCGTATGG AGTTGACTCG TATTATCGAA 
ATCGTCACTT CACAAAAGCT CAACAATGAG GATTACTTAC AATTTGGTAA TGCCACATTC 
CCACGTCTTT CTCCGCCACA CTCGGAACCT GATGCTATTT CGGAGATCCG CTCAGGTTTT 
GCGAAAGTAT CTCGTCTATC GCAAAACGGA GGTGGCAAAG CTTTCATGAC GGTTGATACG 
AAGATTTCTC CATTTTACAA GGATACATCA GTTATCAAGT TTTCAAGCAA CAAGTTATCC 
GAAATGAAAG GTGGAGGCGG TGGAAGAGGC GGATACGGAA GAAGTGACTC GCGAGACTCT 
CGTGGTGGAT ACCGTGGCGG CCGTTCCGAC TCGAGAGACT TTCGTGGTGT CTATGGAAAC 
CGAGGTGGCA ACGACAGGTA CCGAGATGAA AGCAGAGGAC GTCGCGACAT GTACGATTCC 
CGTCGAGACA GTGGTTCTTC AAATGGAGCG GACTACTCTC CGAGTGATGC TGCTGAGTTG 
GAACACGCTT TTGGCGAAAG AGGAAACACG AAGCGATGCA TTGAAGAAGC ACTCAAAGGA 
TTGGACGTGG AATGCACTCA CTTGAAGGGT AACCTTATTC GCGTATCCTC GATTGCCGAG 
AACAATGCTG AGAACACTTC GTTCATGATG AAGGACGACA AGGGAGAACG CGAGGTCACC 
GTGGCGGAAT ATTTCCTGTT GCAGTACAAC ATCAAGCTGA AATATCCGAG ACTTCCTCTC 
GTCGTCTCAA AAAGATTCAA ACATGAATCT TTCTTCCCAA TGGAGTTGCT TCGAATTGCT 
CCAGGCCAGC GGATCAAGGT CAACAAGATG AGCCCGACAG TACAATCGGC GATGACAGGA 
AGAAATGCGT CTATGCCACA GCATCATGTG AAGTTGGTTC AAGATATTCT CCGCGACAAC 
TTGAAGCTTG AACAAAATAA GTACATGGAT GCTTTCGGAA TCAAACTGAT GTCGACTGAG 
CCAATCCAAA TGACTGCGAA GCTCCTCCCA CCAGCTCAAA TCAAGTTCAA GGGCCAGACT 
TACATGCCTG ACATGAGCCG TCCAGCGTTC CGCACACAGG ACAAGTTTGT CGAACCAGCA 
CGGATCAGAA AAATTGGTAT TGTTGTCTTT GACAACTGTA TCCAAATGAG ACAGGCCGAA 
GATTTCTGCG ACAAGCTGTC CAATTTCTGT CGTGACAATG GAATCACGGT CGAAAAGGAC 
TCGCGAGATT GGTCCATCCG TGAATTGAAC TCCAGTGATT CCGTTGCTAT CCAAAATCTG 
ATGAAGAAAT GGCTCGATGA TAGAGTGGAC ATTCTTGTTG GAATAGCAAG GGAGAAGAAG 
CCGGATGTTC ACGACATACT CAAATACTTT GAGGAATCGA TTGGCCTGCA AACAATCCAA 
TTGTGTCAAC AGACTGTCGA TAAAATGATG GGAGGTCAAG GTGGACGCCA AACGATTGAC 
AACGTCATGA GAAAGTTCAA CCTCAAGTGT GGTGGAACCA ACTTCTTCGT CGAGATCCCG 
AATGCTGTGC GTGGAAAAGC AGTGTGCTCA AACAATGAAA CATTGAGAAA GAAACTTCTC 
GAACATGTAC AATTCATCGG ATTCGAGATT TCTCATGGCG CATCACGCAC TTTGTTCGAT 
CGCAGTAGAT CACAAATGGA TGGTGAGCCA TCAGTTGTTG GAGTCTCGTA TTCCCTGACC 
AACTCGACGC AGCTCGGAGG CTTCACATAC CTTCAAACCC AAAAGGAGTA CAAACTTCAA 
AAACTTGATG AATTCTTCCC AAAGTGTGTC AGATCGTACA AGGAGCATTC GAAGACACTC 
CCAACAAGAA TCGTCATCTA TCGTGTTGGT GCTGGAGAAG GAAACTTCAA TCGTGTTAAG 
GAAGAAGTCG AAGAGATGCG ACGAACATTT GACAAGATTC AGCCCGGATA CCGTCCACAT 
CTCGTGGTGA TCATCGCACA AAGAGCTTCA CATGCTCGCG TTTTTCCATC ATGTATCAGT 
GGCAACAGAG CGACGGATCA GAACATCCCA TCTGGAACAT GTGTCGAGAA TGTCCTGACA 
TCCTACGGAT ACGACGAGTT CATTTTGTCT TCACAGACGC CGCTTATTGG TACCGTTCGT 
CCGTGCAAGT ACACGATCCT TGTCAATGAT GCCAAATGGT CTAAAAACGA GCTCATGCAC 
TTGACGTACT TCCGCGCATT CGGTCATCAA GTGTCCTACC AGCCACCATC GGTTCCAGAT 
GTTCTTTATG CGGCAGAAAA TCTGGCTAAA CGAGGACGCA ACAATTACAA AATTCATCAG 
CGTTACGTGA ATTTGCAAGC AGTTGAGAAC CGCATCATCA AAGACCATTC GGAACTCATC 
AACGAAGACA TGCGAGAAGA ACTGGCGGCA GCCATTGTTG ATGAGATGTC CGTTGCAATG 
AACGGAATGA CCATTCCGAA GCGAAACTTC TGGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hrde-1 ( NM_065433.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_497834.1 Caenorhabditis elegans Heritable RNAi Deficient (hrde-1), mRNA.

Price & Availability↑

CloneID	OCa341988
Clone ID Related Accession (Same CDS sequence)	NM_065433.4 , NM_065433.5
Accession Version	NM_065433.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Heritable RNAi Deficient
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_065433.4.

ATGGCCGACT TGCTCGACAA AATTATGGGA AGTTCGTCTT CCAACAGGAT TCCCAAACGA 
GACAACCGAA TGAATCAGGA CAAGGACGAA CCGACGTCAA AGAGAAGTGC ACCAATGTTC 
TCTACTCCGA AGTCAACAAC ACCAATTGGA CGGGACTCTT TCGCAACGAT GGACACGATG 
AATGTTCAGA TGAACATGTT TCCAGTGGAC ATCAAGGATA TGCCCCACAA GATCCAACGC 
TTTCAAGTTG ACGTCATCGT CTGTTCCAGC AACGGAAAAC AGATCAATGC CAACCTTGGA 
GTTTTGGCGG CCAAGGGAGA TGTCAATTCC CATAACCGTC GCTTGGCACA ATACTACATC 
ATGAGAACAG TACATGACAA GCTTCTGGTG AAGTTCAGTG GAAAGAGTCA CCACTTCCTT 
GCTTACGACT GCGCTGCGAC TCTCTATCTT CCGGAGGGAG TCTACACTGG TGACGATCAA 
GAAGAAGTCA CGTTGACGAT CGATGACTTC CCGAAGGAAG AATGGAAATT CGTGTCGAAG 
TTGTCTCGCA GAAAGGACGA TTCTTACCTT GTTGTTCTCA AACCTGCCGG ATTTGTGTAC 
ACCCAAGGAG AAGTTGCCCA GGAGGAGGCT AATCGTATGG AGTTGACTCG TATTATCGAA 
ATCGTCACTT CACAAAAGCT CAACAATGAG GATTACTTAC AATTTGGTAA TGCCACATTC 
CCACGTCTTT CTCCGCCACA CTCGGAACCT GATGCTATTT CGGAGATCCG CTCAGGTTTT 
GCGAAAGTAT CTCGTCTATC GCAAAACGGA GGTGGCAAAG CTTTCATGAC GGTTGATACG 
AAGATTTCTC CATTTTACAA GGATACATCA GTTATCAAGT TTTCAAGCAA CAAGTTATCC 
GAAATGAAAG GTGGAGGCGG TGGAAGAGGC GGATACGGAA GAAGTGACTC GCGAGACTCT 
CGTGGTGGAT ACCGTGGCGG CCGTTCCGAC TCGAGAGACT TTCGTGGTGT CTATGGAAAC 
CGAGGTGGCA ACGACAGGTA CCGAGATGAA AGCAGAGGAC GTCGCGACAT GTACGATTCC 
CGTCGAGACA GTGGTTCTTC AAATGGAGCG GACTACTCTC CGAGTGATGC TGCTGAGTTG 
GAACACGCTT TTGGCGAAAG AGGAAACACG AAGCGATGCA TTGAAGAAGC ACTCAAAGGA 
TTGGACGTGG AATGCACTCA CTTGAAGGGT AACCTTATTC GCGTATCCTC GATTGCCGAG 
AACAATGCTG AGAACACTTC GTTCATGATG AAGGACGACA AGGGAGAACG CGAGGTCACC 
GTGGCGGAAT ATTTCCTGTT GCAGTACAAC ATCAAGCTGA AATATCCGAG ACTTCCTCTC 
GTCGTCTCAA AAAGATTCAA ACATGAATCT TTCTTCCCAA TGGAGTTGCT TCGAATTGCT 
CCAGGCCAGC GGATCAAGGT CAACAAGATG AGCCCGACAG TACAATCGGC GATGACAGGA 
AGAAATGCGT CTATGCCACA GCATCATGTG AAGTTGGTTC AAGATATTCT CCGCGACAAC 
TTGAAGCTTG AACAAAATAA GTACATGGAT GCTTTCGGAA TCAAACTGAT GTCGACTGAG 
CCAATCCAAA TGACTGCGAA GCTCCTCCCA CCAGCTCAAA TCAAGTTCAA GGGCCAGACT 
TACATGCCTG ACATGAGCCG TCCAGCGTTC CGCACACAGG ACAAGTTTGT CGAACCAGCA 
CGGATCAGAA AAATTGGTAT TGTTGTCTTT GACAACTGTA TCCAAATGAG ACAGGCCGAA 
GATTTCTGCG ACAAGCTGTC CAATTTCTGT CGTGACAATG GAATCACGGT CGAAAAGGAC 
TCGCGAGATT GGTCCATCCG TGAATTGAAC TCCAGTGATT CCGTTGCTAT CCAAAATCTG 
ATGAAGAAAT GGCTCGATGA TAGAGTGGAC ATTCTTGTTG GAATAGCAAG GGAGAAGAAG 
CCGGATGTTC ACGACATACT CAAATACTTT GAGGAATCGA TTGGCCTGCA AACAATCCAA 
TTGTGTCAAC AGACTGTCGA TAAAATGATG GGAGGTCAAG GTGGACGCCA AACGATTGAC 
AACGTCATGA GAAAGTTCAA CCTCAAGTGT GGTGGAACCA ACTTCTTCGT CGAGATCCCG 
AATGCTGTGC GTGGAAAAGC AGTGTGCTCA AACAATGAAA CATTGAGAAA GAAACTTCTC 
GAACATGTAC AATTCATCGG ATTCGAGATT TCTCATGGCG CATCACGCAC TTTGTTCGAT 
CGCAGTAGAT CACAAATGGA TGGTGAGCCA TCAGTTGTTG GAGTCTCGTA TTCCCTGACC 
AACTCGACGC AGCTCGGAGG CTTCACATAC CTTCAAACCC AAAAGGAGTA CAAACTTCAA 
AAACTTGATG AATTCTTCCC AAAGTGTGTC AGATCGTACA AGGAGCATTC GAAGACACTC 
CCAACAAGAA TCGTCATCTA TCGTGTTGGT GCTGGAGAAG GAAACTTCAA TCGTGTTAAG 
GAAGAAGTCG AAGAGATGCG ACGAACATTT GACAAGATTC AGCCCGGATA CCGTCCACAT 
CTCGTGGTGA TCATCGCACA AAGAGCTTCA CATGCTCGCG TTTTTCCATC ATGTATCAGT 
GGCAACAGAG CGACGGATCA GAACATCCCA TCTGGAACAT GTGTCGAGAA TGTCCTGACA 
TCCTACGGAT ACGACGAGTT CATTTTGTCT TCACAGACGC CGCTTATTGG TACCGTTCGT 
CCGTGCAAGT ACACGATCCT TGTCAATGAT GCCAAATGGT CTAAAAACGA GCTCATGCAC 
TTGACGTACT TCCGCGCATT CGGTCATCAA GTGTCCTACC AGCCACCATC GGTTCCAGAT 
GTTCTTTATG CGGCAGAAAA TCTGGCTAAA CGAGGACGCA ACAATTACAA AATTCATCAG 
CGTTACGTGA ATTTGCAAGC AGTTGAGAAC CGCATCATCA AAGACCATTC GGAACTCATC 
AACGAAGACA TGCGAGAAGA ACTGGCGGCA GCCATTGTTG ATGAGATGTC CGTTGCAATG 
AACGGAATGA CCATTCCGAA GCGAAACTTC TGGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_497834.1
CDS	7..3105
Translation	MADLLDKIMGSSSSNRIPKRDNRMNQDKDEPTSKRSAPMFSTPKSTTPIGRDSFATMDTMNVQMNMFPVDIKDMPHKIQRFQVDVIVCSSNGKQINANLGVLAAKGDVNSHNRRLAQYYIMRTVHDKLLVKFSGKSHHFLAYDCAATLYLPEGVYTGDDQEEVTLTIDDFPKEEWKFVSKLSRRKDDSYLVVLKPAGFVYTQGEVAQEEANRMELTRIIEIVTSQKLNNEDYLQFGNATFPRLSPPHSEPDAISEIRSGFAKVSRLSQNGGGKAFMTVDTKISPFYKDTSVIKFSSNKLSEMKGGGGGRGGYGRSDSRDSRGGYRGGRSDSRDFRGVYGNRGGNDRYRDESRGRRDMYDSRRDSGSSNGADYSPSDAAELEHAFGERGNTKRCIEEALKGLDVECTHLKGNLIRVSSIAENNAENTSFMMKDDKGEREVTVAEYFLLQYNIKLKYPRLPLVVSKRFKHESFFPMELLRIAPGQRIKVNKMSPTVQSAMTGRNASMPQHHVKLVQDILRDNLKLEQNKYMDAFGIKLMSTEPIQMTAKLLPPAQIKFKGQTYMPDMSRPAFRTQDKFVEPARIRKIGIVVFDNCIQMRQAEDFCDKLSNFCRDNGITVEKDSRDWSIRELNSSDSVAIQNLMKKWLDDRVDILVGIAREKKPDVHDILKYFEESIGLQTIQLCQQTVDKMMGGQGGRQTIDNVMRKFNLKCGGTNFFVEIPNAVRGKAVCSNNETLRKKLLEHVQFIGFEISHGASRTLFDRSRSQMDGEPSVVGVSYSLTNSTQLGGFTYLQTQKEYKLQKLDEFFPKCVRSYKEHSKTLPTRIVIYRVGAGEGNFNRVKEEVEEMRRTFDKIQPGYRPHLVVIIAQRASHARVFPSCISGNRATDQNIPSGTCVENVLTSYGYDEFILSSQTPLIGTVRPCKYTILVNDAKWSKNELMHLTYFRAFGHQVSYQPPSVPDVLYAAENLAKRGRNNYKIHQRYVNLQAVENRIIKDHSELINEDMREELAAAIVDEMSVAMNGMTIPKRNFWA

Target ORF information:

RefSeq Version	NM_065433.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Heritable RNAi Deficient (hrde-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065433.5

ORF Insert Sequence:

ATGGCCGACT TGCTCGACAA AATTATGGGA AGTTCGTCTT CCAACAGGAT TCCCAAACGA 
GACAACCGAA TGAATCAGGA CAAGGACGAA CCGACGTCAA AGAGAAGTGC ACCAATGTTC 
TCTACTCCGA AGTCAACAAC ACCAATTGGA CGGGACTCTT TCGCAACGAT GGACACGATG 
AATGTTCAGA TGAACATGTT TCCAGTGGAC ATCAAGGATA TGCCCCACAA GATCCAACGC 
TTTCAAGTTG ACGTCATCGT CTGTTCCAGC AACGGAAAAC AGATCAATGC CAACCTTGGA 
GTTTTGGCGG CCAAGGGAGA TGTCAATTCC CATAACCGTC GCTTGGCACA ATACTACATC 
ATGAGAACAG TACATGACAA GCTTCTGGTG AAGTTCAGTG GAAAGAGTCA CCACTTCCTT 
GCTTACGACT GCGCTGCGAC TCTCTATCTT CCGGAGGGAG TCTACACTGG TGACGATCAA 
GAAGAAGTCA CGTTGACGAT CGATGACTTC CCGAAGGAAG AATGGAAATT CGTGTCGAAG 
TTGTCTCGCA GAAAGGACGA TTCTTACCTT GTTGTTCTCA AACCTGCCGG ATTTGTGTAC 
ACCCAAGGAG AAGTTGCCCA GGAGGAGGCT AATCGTATGG AGTTGACTCG TATTATCGAA 
ATCGTCACTT CACAAAAGCT CAACAATGAG GATTACTTAC AATTTGGTAA TGCCACATTC 
CCACGTCTTT CTCCGCCACA CTCGGAACCT GATGCTATTT CGGAGATCCG CTCAGGTTTT 
GCGAAAGTAT CTCGTCTATC GCAAAACGGA GGTGGCAAAG CTTTCATGAC GGTTGATACG 
AAGATTTCTC CATTTTACAA GGATACATCA GTTATCAAGT TTTCAAGCAA CAAGTTATCC 
GAAATGAAAG GTGGAGGCGG TGGAAGAGGC GGATACGGAA GAAGTGACTC GCGAGACTCT 
CGTGGTGGAT ACCGTGGCGG CCGTTCCGAC TCGAGAGACT TTCGTGGTGT CTATGGAAAC 
CGAGGTGGCA ACGACAGGTA CCGAGATGAA AGCAGAGGAC GTCGCGACAT GTACGATTCC 
CGTCGAGACA GTGGTTCTTC AAATGGAGCG GACTACTCTC CGAGTGATGC TGCTGAGTTG 
GAACACGCTT TTGGCGAAAG AGGAAACACG AAGCGATGCA TTGAAGAAGC ACTCAAAGGA 
TTGGACGTGG AATGCACTCA CTTGAAGGGT AACCTTATTC GCGTATCCTC GATTGCCGAG 
AACAATGCTG AGAACACTTC GTTCATGATG AAGGACGACA AGGGAGAACG CGAGGTCACC 
GTGGCGGAAT ATTTCCTGTT GCAGTACAAC ATCAAGCTGA AATATCCGAG ACTTCCTCTC 
GTCGTCTCAA AAAGATTCAA ACATGAATCT TTCTTCCCAA TGGAGTTGCT TCGAATTGCT 
CCAGGCCAGC GGATCAAGGT CAACAAGATG AGCCCGACAG TACAATCGGC GATGACAGGA 
AGAAATGCGT CTATGCCACA GCATCATGTG AAGTTGGTTC AAGATATTCT CCGCGACAAC 
TTGAAGCTTG AACAAAATAA GTACATGGAT GCTTTCGGAA TCAAACTGAT GTCGACTGAG 
CCAATCCAAA TGACTGCGAA GCTCCTCCCA CCAGCTCAAA TCAAGTTCAA GGGCCAGACT 
TACATGCCTG ACATGAGCCG TCCAGCGTTC CGCACACAGG ACAAGTTTGT CGAACCAGCA 
CGGATCAGAA AAATTGGTAT TGTTGTCTTT GACAACTGTA TCCAAATGAG ACAGGCCGAA 
GATTTCTGCG ACAAGCTGTC CAATTTCTGT CGTGACAATG GAATCACGGT CGAAAAGGAC 
TCGCGAGATT GGTCCATCCG TGAATTGAAC TCCAGTGATT CCGTTGCTAT CCAAAATCTG 
ATGAAGAAAT GGCTCGATGA TAGAGTGGAC ATTCTTGTTG GAATAGCAAG GGAGAAGAAG 
CCGGATGTTC ACGACATACT CAAATACTTT GAGGAATCGA TTGGCCTGCA AACAATCCAA 
TTGTGTCAAC AGACTGTCGA TAAAATGATG GGAGGTCAAG GTGGACGCCA AACGATTGAC 
AACGTCATGA GAAAGTTCAA CCTCAAGTGT GGTGGAACCA ACTTCTTCGT CGAGATCCCG 
AATGCTGTGC GTGGAAAAGC AGTGTGCTCA AACAATGAAA CATTGAGAAA GAAACTTCTC 
GAACATGTAC AATTCATCGG ATTCGAGATT TCTCATGGCG CATCACGCAC TTTGTTCGAT 
CGCAGTAGAT CACAAATGGA TGGTGAGCCA TCAGTTGTTG GAGTCTCGTA TTCCCTGACC 
AACTCGACGC AGCTCGGAGG CTTCACATAC CTTCAAACCC AAAAGGAGTA CAAACTTCAA 
AAACTTGATG AATTCTTCCC AAAGTGTGTC AGATCGTACA AGGAGCATTC GAAGACACTC 
CCAACAAGAA TCGTCATCTA TCGTGTTGGT GCTGGAGAAG GAAACTTCAA TCGTGTTAAG 
GAAGAAGTCG AAGAGATGCG ACGAACATTT GACAAGATTC AGCCCGGATA CCGTCCACAT 
CTCGTGGTGA TCATCGCACA AAGAGCTTCA CATGCTCGCG TTTTTCCATC ATGTATCAGT 
GGCAACAGAG CGACGGATCA GAACATCCCA TCTGGAACAT GTGTCGAGAA TGTCCTGACA 
TCCTACGGAT ACGACGAGTT CATTTTGTCT TCACAGACGC CGCTTATTGG TACCGTTCGT 
CCGTGCAAGT ACACGATCCT TGTCAATGAT GCCAAATGGT CTAAAAACGA GCTCATGCAC 
TTGACGTACT TCCGCGCATT CGGTCATCAA GTGTCCTACC AGCCACCATC GGTTCCAGAT 
GTTCTTTATG CGGCAGAAAA TCTGGCTAAA CGAGGACGCA ACAATTACAA AATTCATCAG 
CGTTACGTGA ATTTGCAAGC AGTTGAGAAC CGCATCATCA AAGACCATTC GGAACTCATC 
AACGAAGACA TGCGAGAAGA ACTGGCGGCA GCCATTGTTG ATGAGATGTC CGTTGCAATG 
AACGGAATGA CCATTCCGAA GCGAAACTTC TGGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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hrde-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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