lov-1 cDNA ORF clone, Caenorhabditis elegans

The following lov-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lov-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa337623 NM_063783.4
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Caenorhabditis elegans Location of vulva defective 1 (lov-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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OCa337623 NM_063783.3 Caenorhabditis elegans Location of vulva defective 1 (lov-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa337623
    Clone ID Related Accession (Same CDS sequence) NM_063783.3 , NM_063783.4
    Accession Version NM_063783.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Location of vulva defective 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_063783.2. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGAAGAAGT CAAACTTCTT TGTTCTATTG CTACTGGCAA TATCGGCGAT ACAAATTGAT 
    GGATTGCATT ACCAACTTCT TGACGGGATC GCAACTTTTC GATTAGACAA CGATGACACA
    ACAATTGGAG GAGTTCCAAG GAATTCACAA GGAGTCGTTA AAATCAAATT GAGCTGCGGA
    TTGAATAGAC TTTCAGTTGA GAACAAGGTT ACGGAAGTTT CAAGTTTGGA ATTGATTCAC
    AACTGCATCC AAACAGAGAC CAGGCTTGTC GGACTCTTTT TGAACTCAAC TTGGATTACT
    TTGAATGAAG TGAATGATGA CGATGAGATC AGTATTGCTG TGGAGGCGAA ATATGAAGTT
    TGTTATGATG ACGGTATCGA CAGATGTGAT GGCTCCCTAT GGTGGCTCCA AGTCGGTGGA
    AACGAGATGG CGTTGCTGGG CTACCGAGAG AAGTGTGAGA GCGGAGAGAT CAACGAAGAG
    TATGCGAGAA GAATGTGCAA GCGACCTTAT CGTTCTGAAA AGAGCACAGC TATCAGTGAT
    AGTCAAGGAG TGTATTATGA TGGGCAGGTA CTTAAGGGTG TCAGGGCAAA ACAGTTTAGC
    ATGAGGACTT CTGGATCACC TACTTTGAGA AGAATGAAAC GAGACGCTGG TGATAATACT
    TGCGACTACA CTATTGAGTC AACTTCAACC TCGACTACAA CTCCAACTAC CACAACCGTG
    ACTAGTACCG TGACATCTAC AACAACCGTC CCAACAAGTA CAAGTACGGT GACTACCGCA
    ATGAGCACAA GCACATCAAC TCCATCAACA TCCACCACCA TCGAATCCAC GTCAACAACG
    TTCACTTCCA CAGCATCCAC GTCGACATCA TCAACTAGCA CAACTCAGCA GAGTTCTAGC
    ACAATCACAA GCTCGCCGAG CTCCACCACA CTGAGTACAA GTATTCCAAC CACTACAACA
    CCCGAAATCA CATCAACTTT GTCGTCTCTC CCGGATAATG CGATTTGCTC CTACCTTGAC
    GAGACCACTA CGTCTACCAC TTTCACCACC ACTATGTTGA CATCCACTAC TACCGAAGAA
    CCATCTACTT CCACAACCAC AACAGAAGTT ACATCTACAT CTTCTACTGT TACAACAACT
    GAACCAACCA CTACTTTGAC CACCTCGACT GCTTCAACGT CAACAACCGA ACCATCGACG
    AGTACTGTAA CTACTTCCCC GTCAACATCC CCTGTGACTT CTACGGTGAC ATCTAGTTCA
    AGCTCCTCGA CTACTGTAAC GACCCCAACA AGCACTGAGT CCACATCGAC ATCTCCTTCG
    TCTACAGTAA CAACCAGCAC AACTGCTCCT TCAACATCAA CTACTGGGCC ATCGAGTTCT
    TCTTCCACTC CAAGTTCAAC GGCTTCTTCG TCTGTGTCAA GCACCGCTTC TTCTACTCAA
    TCATCCACGT CTACTCAGCA AAGTTCAACA ACAACAAAAT CTGAGACAAC TACTAGCTCG
    GACGGAACTA ATCCAGACTT CTATTTTGTA GAAAAGGCAA CTACAACGTT CTATGATTCC
    ACAAGTGTGA ACCTAACGCT TAATTCCGGC CTCGGAATCA TTGGATATCA AACATCCATC
    GAATGTACAA GCCCTACATC TTCTAATTAT GTTTCAACCA CTAAAGACGG AGCATGTTTC
    ACGAAAAGTG TATCAATGCC ACGTCTCGGA GGGACGTACC CTGCTAGCAC ATTCGTTGGT
    CCTGGAAACT ACACCTTCAG AGCAACTATG ACAACCGACG ATAAAAAAGT TTACTACACG
    TATGCGAACG TTTATATTCA GGAATATTCT TCCACCACCA TAGAGTCGGA ATCTTCTACA
    TCTGCTGTAG CAAGCTCTAC GTCATCTACA CCATCAACTC CATCTTCCAC ATTATCAACA
    TCCACAGTCA CAGAGCCGTC ATCCACACGT TCAAGCGATT CTACGACAAC GTCGGCTGGG
    AGCACAACTA CTTTACAAGA ATCCACAACA ACTTCCGAAG AAAGTACTAC TGATTCCAGT
    ACAACTACCA TCTCGGACAC ATCCACAACT TCTTCCAGTC CGAGTTCTAC CACTGCTGAT
    TCAACTTCCA CTTTGTCAGT CGATCAGTTC GATTTCATCT TGGACAGTGG ATTGTCATGG
    AACGAGACAC GTCACAATGA AGACAGCATC AACATTGTAC CTTTACCAAC TAATGCCATT
    ACTCCTACTG AGAGAAGCCA AACATTTGAA TGCAGAAATG TGTCTACTGA GCCTTTTCTT
    ATAATTAAGG AATCCACTTG CTTAAACTAC TCAAACACGG TCCTAAACGC AACTTATTCT
    TCTAATATTC CAATTCAACC AATTGAAACA TTTCTTGTTG GCATTGGCAC CTATGAGTTT
    CGAATCAACA TGACGGATTT GACGACAATG CAAGTGGTCA GCCACATTTT CACATTGAAT
    GTTGTTGCGG ATTCAACAAG TACATCCGAA GTTACCAGTA CAACTTCAAC TGGATCTTCT
    TCCGAAAGTT CAGCCATTTC CACAACTTCA GGAATTGAAT CTACGTCGAC TTTGGAAGCT
    AGCACGACTG ATGCTTCACA AGACAGTTCT ACATCAACAT CGGATTCTGG CACAACTTCA
    GATTCCACCA CCATAGACTC ATCTAACTCA ACTCCATCCA CTTCAGATTC ATCTGGTTTA
    TCTCAAACAC CATCCGATTC ATCAAGTGCA TCTGATAGCA TGAGAACAAC AACTGTGGAT
    CCAGACGCAA GTACAGAAAC CCCTTACGAC TTTGTATTGG AAAATTTGAC ATGGAACGAG
    ACAGTGTATT ACTCTGAAAA TCCATTTTAT ATTACACCAA TTCCAAACAA AGAGCCCGGA
    GCTTTAACGA CTGCTATGAC CTGTCAATGT CGAAACGATT CTTCTCAGCC ATTTGTTTTA
    CTGAAGGAAA GCAATTGCTT GACAGAGTTT GGAAAAAATG GTGCATATAG TGCCTCCGTA
    TCTTTCAACC CAATGACCAG CTTTGTACCT GCAACTGGAA CTTATGAATT CTTAATTAAT
    GTTACCAATC GTGCATCTGG AGAATCTGCC AGTCACATCT TTACTATGAA TGTAGTTCTT
    CCAACTACAA CTACTGAAAC CCCACCAACC ACTGTCAGCT CGTCGGATGA TGCCGGTGGC
    AAAACCGGTG GAACTGGAGC TACTGGGGGA ACTGGTGGAA CAGGTTCAGG AGGATCTGCG
    ACAACTCTTA GCACGGGTGA TGCAGTCAGA TCAACCACTT CTGGATCTGG AAGTGGACAA
    TCATCAACGG GATCGGGGGC TGGTGGATCA GGAACGACCG CTTCTGGAAG TGGGTCAGGG
    GGGTCTTCAG GGACGGGTTC GGACGGGGTA AACTCTGGAA AAACAACGGC ATTGAATGGA
    GATGGTACAG GATCTGGAAC AGCTACCACA CCAGGATCCC ATTTAGGAGA CGGTGGTAGT
    ACATCTGGCT CAGGATCAGA TTCAAATGGA TCTTCTGGTG TTTCAACCAA GAGCTCATCT
    GGCTCCGATA CGTCTGGATC TTCCGATTCC TCTGGTGCAA ATGGTGCATT CTCTGCTACC
    GCGCAACCAT CTACAAGAAC TACTAAAACT CGCAGCTCTC TTGCAACAGT TTCTCCAATT
    TCTGCAGCTG AACAAGCGAT CATAGATGCT CAAAAAGCTG ATGTAATGAA CCAACTGGCT
    GGGATAATGG ATGGATCTGC ATCCAACAAC TCTCTAAACA CAAGCAGCTC TCTTCTCAAT
    CAAATCTCAT CTCTACCTGC CGCAGATCTA GTGGAAGTTG CTCAAAGTTT GCTCTCAAAT
    ACCCTCAAAA TCCCTGGAGT CGGAAATATG TCAAGTGTTG ATGTTTTGAA AACATTGCAA
    GACAATATTG CAACAACAAA TTCGGAATTG GCCGACGAAA TGGCAAAAGT TATCACTAAA
    CTAGCAAATG TCAATATGAC TTCCGCACAA TCCTTGAACT CCGTACTGTC TTCTCTCGAT
    CTTGCTCTCA AAGGAAGTAC AGTCTATACC TTGGGAGTAT CAAGTACAAA ATCAAAAGAT
    GGAACTTACG CGGTTATTTT TGGATATGTT ATTGCATCGG GATATACATT GGTATCGCCT
    AGGTGTACGC TCAGCATTTA CGGATCTACA ATATACCTTA CAGGAGATAC CCGGGCATCT
    TATAAACAGC TTGATGGAGA TACCGTTACT GCGGACACTA TGCTAGCAGC TGCCATCGGA
    ATCCAAGGAA TGTTCGCAAC AAATGGAAGG ACTGTGCAAG TTGAGCAGGA TAAAATAGAT
    GACAAACGAT CACTGGTTTC TGGAAATATA ATGGCTACAA TGAGTGGAGT TGGAGATGTT
    CAATCAGGGG AATATAGTTA TAATGATATG TATGTAACTG CTTGGAATGT CACTTATGAC
    AATTCAACAG TAGGATCCAC AAGTCAGAAG AACACTTCAT TTTCGTTCAA TATCCCAGTC
    TCAGAAGTTC AATACATATT ATTGATTGAA TCTGGAACAA TGATAAAACT GCATTCTACT
    CAAAATATTG TATCTAGAGG ACTAGTAGTA ACTGCATCAT ATGGTGGAGT TACTTACACA
    ATAACTTGCA CGAATGGAAC AGGAAAGTTT GTTGAAGTAG ACACAGATAA TGCCATTTTC
    TCATACAATG CTGACAGCTT CACGGTTGTG GCATCAGATG GATCCTCAGC GAGCACGGTT
    AAGAAGTTGA TTCAGATGCC GATAGTTATT GAGAACGTCA ACCTTGCTTT ATTCAATCAA
    ACTACTTCTC CACTTGTGTT CTCAAACGCG GGAAGTTATT CAATGCGAAT GGTGCTGTCT
    CCGCAGGACA TTGGAATTCC AGCTGTCTCT GCTTTATCTC AAACTGTCTC AATATCTACT
    CTTTCACCGA CGGCTTCGTA CACCAAAGAT GATTTGCAAT CATTGATTAA GGAACAAACA
    CTTGTCACCG TTTCGGGAAC TCTATTCTTC TCCAAAGCGT CATCCATCGA TGTCAGCGGC
    TACTCATTTT TCGTAGACTC CACCGCCCTT TATTTGTCAA ACTCTGTGAC AACTCTGGTG
    ATCAGTTCTC CCACCTACAA TATTGTTTCT TTGGCGCTTG GAGGTTACGG TATTCAGATA
    ACAGCCGGAA CTTACACATC TGGTTCTCAA ACTCATACGG TAACTTTAAT GGAATTCTCT
    GACACACAGA AAATGAGAAT CGATGGAGGG TTGATTATTA GAAATGGAAC GAATGGATAT
    GTAATTCAGA ATGGACAAGT TTCGACGGAA GGCGATGTTT CGGGAACTAA AATTGATATT
    GTGCCGCAAA GTTTGATGAA TCAAGAATCC CAGCAACAAA TTGAAACGAT TCTTAGCAAT
    ACTCAAGATT TTCTTACAAA TAATGGAATG ACTATGACAG ATGCAGAGAT TAATGATACA
    TCCAACTCTC TCCTTTCAAT TGCGGGATCT TTAACATCAG CGTTGAAGAT TGCTCTCGAC
    AATCCTCTTT CTTCTGATCT AGCTGCCAAT TTGAAATATG CAACAGATAA CTATGATTCC
    TTGTACAACG TGCTACCAAG TGATCCAGAC AATATTGTGT ACGTGGAGGA AATGACGTCA
    GAAGAATGGG CTGCTTACGT CACAAAAATG TTCCAGAAGA ACATCGCCAA AAATCTAGCC
    AACCAACTTG CATCTACACT AGATACCCTG GAAAACACGC TAGCAGCTAG AGCTATAGCA
    ACTGGCAACT TACCTTATGA CTACTCGAAT AGCGTCGACG GAACCGGAAT GGTAATCGTT
    ATCGATGATG CATCAAATAT TGTAGGAAAA ACTCAAAACT GTGAAGAATG GGCATTCAAA
    CTTCCATCGC CCGCTTCAAC TCTGAATACT GCTGAGATCA CAGATAAAAC ACTGATTCAA
    GTTGGATTAG TTTGCTATGC CACAAACCCG AGAACTTATG TGGATAACTT TGATATGTTA
    ATTACCTCAG GTGCACTTGA AGCACATATC AAAGATGAGA ATCAGATAAT CATTCCAATA
    ACCGGAACTA CTGCTCCAAT TTATGTGAAC GGCAGGGGAA GTGAGGATGA TGCAGTGCTA
    ACTTTAATGC AACAAGGAGA CTTTGCCAGT TATCAGATAT TAGATCTTCA TGCTTTTCGA
    ACCACTAACT GGAACAACTC GCTTCAAGTA GAGATTATTG CATCTCAAGA CTATGAAATT
    CCAAACAACG ATGACACCTA CATGTTCTCA TCATTCCAAA GTCTTCCTGG CCCACTTGAA
    TCAAATCATG AATGGATCTT TGACTTGAAC ACATTGAACA AGACAAGCAA TTATTTTGTC
    ACCGCCGGAA ATTTGATCAA CAACACTGGA CTTTTCTTTA TTGGAATTGG AAAAAGAAAT
    AGCTCTACTA ACACTGGAAA TAGTTCGGAT ATTGTAAACT ATGGTCAGTA CGATTCAATG
    CAGTGGTCAT TTGCACGAAG TGTACCAATG GATTATCAAG TCGCTGCAGT GAGTAAAGGA
    TGTTACTTCT ATCAGAAAAC ATCGGATGTC TTCAATTCTG AAGGAATGTA TCCAAGTGAT
    GGACAAGGAA TGCAGTTCGT TAATTGTTCA ACTGATCATT TAACAATGTT CTCTGTTGGT
    GCATTCAATC CAACTATTGA TGCAGATTTC TCTTACAATT ATAATGTGAA TGAAATCGAA
    AAGAATGTGA AAGTGATGAT TGCCGCTGTT TTCATGCTAG TAGTTTACGG ATGTCTTACC
    ATCAATGCAA TCATCTGTCA ACGTAAAGAT GCATCACGCG GGAGACTTCG TTTTCTGAAA
    GACAATGAGC CTCATGATGG ATACATGTAT GTGATTGCCG TGGAGACTGG ATATCGAATG
    TTTGCAACTA CGGATAGTAC GATATGTTTC AATTTATCTG GAAATGAAGG GGATCAGATT
    TTCCGATCGT TCAGATCAGA AGAAGATGGT AATTGGGAGT TTCCGTTTTC ATGGGGAACC
    ACTGATCGAT TTGTGATGAC AACGGCATTC CCTCTTGGCG AGTTGGAATA CATGAGACTC
    TGGCTTGACG ATGCTGGTCT AGATCACCGA GAGTCGTGGT ATTGCAATCG AATAATTGTG
    AAAGATCTAC AGACTCAGGA TATCTACTAC TTCCCTTTCA ACAATTGGCT TGGAACTAAA
    AACGGAGACG GAGAGACTGA AAGACTAGCG CGAGTGGAGT ATAAGCGAAG GTTTTTGGAT
    GAGTCAATGA GTATGCATAT GCTGGCTCAA ACAATTTCCT GGTTTGCCAT GTTTACTGGT
    GGAGGCAATC GGTTGAGGGA TCGAGTGTCA AGGCAAGACT ATTCTGTCAG TATCATCTTC
    TCACTTGTTG TGGTCAGCAT GATTAGCATC ACAATTCTGA AGAGCGATAA TTCGATTATC
    AGTGATTCGA AGAGTGTCTC CGAGTTCACT TTCACTATAA AAGATATTGC ATTCGGAGTT
    GGATTCGGTG TTCTTATCAC TTTCCTCAAC TCATTGCACA TTTTATTATG CACCAAATGT
    CGTTCCCACA GTGAACATTA CTACTACAAA AAACGAAAGA GAGAGGATCC GGAATTCAAG
    GATAATAGTG GTTCATGGCC GATGTTCATG GCTGGGATGG CACGTACCAT CATAGTATTT
    CCAGTTCTCA TGGGTCTTAT TTATATTTCT GGAGCTGGAA TGAGCTTGAT GGATGATTTA
    GCCAACTCTT TCTATATCCG ATTTCTGATC AGTCTGATTC TATGGGCAGT GGTGTTCGAG
    CCAATTAAGG GATTGATTTG GGCGTTTTTA ATTTTGAAAA CCAGAAAGAG TCATAAAATC
    ATTAACAAGT TGGAGGAAGC ATTGCTAAGA GCTAAGCCAG CTGAGACGTT CTTGAGAAAT
    CCCTATGGGA AAATCGAAAA AGGTCTGGGA ACCGAAATTG CTGATGTTAC AAAGCTCCGA
    GACACTGAAA ACCGAAAAAT GAGAGATGAA CAGTTATTTA TAACCATTCG TGATATGCTT
    TGTTTCTTTG CTTCTTTGTA TATTATGGTT ATGCTGACAT ACTATTGCAA AGACCGGCAT
    GGATATTGGT ATCAGTTGGA GATGAGCACA ATCTTGAACA TCAATCAAAA GAACTACGGT
    GACAATACTT TCATGTCAAT CCAACATGCT GACGATTTCT GGGATTGGGC TCGTGAATCT
    CTGGCCACTG CTCTTTTGGC TTCTTGGTAC GACGGAAATC CTGCATACGG GATGAGAGCC
    TACATGAATG ATAAAGTTTC AAGAAGTATG GGAATAGGTA CGATACGGCA GGTTCGGACA
    AAGAAAAGTG CAGAGTGTAC GATGTTCAAG CAGTTTCAAG GATACATCAA TGATTGTGGA
    GAGGAGTTGA CAAGCAAAAA CGAAGAAAAA ACTTTGTACA TGCAAGCTGG TTGGACCGAA
    CTGGAATCCG AGAATGGAAC AGATGCTTCA GATGAATACA CCTACAAGAC TTCTGAAGAA
    TTATCCACTG AAACAGTTTC AGGCCTACTG TATAGCTATT CAGGAGGTGG ATATACTATC
    AGTATGTCTG GTACCCAAGC GGAAATCATC ACTCTCTTCA ATAAACTTGA TTCGGAACGA
    TGGATCGATG ATCATACTCG AGCAGTCATC ATTGAGTTCT CTGCATACAA TGCTCAAATC
    AACTACTTTT CGGTGGTACA GTTGTTGGTG GAGATTCCAA AGTCAGGAAT TTATCTTCCT
    AATAGTTGGG TTGAATCAGT AAGACTTATC AAATCAGAGG GAAGCGATGG AACTGTGGTC
    AAGTATTATG AGATGCTTTA CATCTTTTTC TCAGTTCTCA TATTTGTAAA AGAGATTGTG
    TTCTACTTGT ATGGGCGGTA CAAGGTGATA ACAACAATGA AACCAACGAG AAATCCATTC
    AAAATTGTGT ATCAGCTGGC ACTGGGAAAC TTCTCACCAT GGAACTTCAT GGACTTGATA
    GTTGGAGCTC TCGCTGTTGC ATCGGTCCTA GCCTACACCA TCCGCCAACG AACCACAAAT
    AGAGCAATGG AAGATTTCAA TGCAAACAAT GGAAATTCGT ACATTAATTT GACTGAACAG
    AGAAATTGGG AGATTGTATT CAGTTATTGT CTTGCCGGAG CCGTATTCTT CACTTCTTGT
    AAAATGATTC GAATCTTGAG ATTCAACAGA AGAATTGGAG TATTAGCGGC TACACTGGAT
    AACGCTCTCG GAGCTATTGT GTCATTTGGA ATTGCTTTCC TGTTCTTCAG CATGACATTC
    AACTCTGTAT TGTACGCAGT TTTAGGAAAT AAAATGGGAG GATATCGGAG CTTAATGGCT
    ACCTTTCAAA CAGCATTGGC TGGAATGCTT GGAAAACTTG ATGTTACCTC TATTCAACCG
    ATTTCTCAAT TTGCGTTTGT TGTGATCATG CTTTATATGA TTGCCGGATC AAAGCTCGTC
    CTGCAACTTT ACGTCACTAT AATTATGTTC GAGTTCGAGG AAATTCGTAA TGACAGTGAG
    AAGCAGACCA ATGATTACGA AATAATCGAT CACATAAAAT ACAAGACAAA ACGTCGCTTG
    GGTCTTCTTG AACCAAAAGA TTTTGCTCCA GTTTCAATTG CTGACACTCA AAAAGACTTC
    CGTCTATTCC ACAGCGCTGT TGCCAAAGTC AATCTGCTTC ATCATCGTGC CACCAGAATG
    CTCCAAACTC AAGGTCAATA CCAAAACCAA ACAGTCATCA ACTACACACT CTCCTATGAT
    CCTGTCTCTG CTATTCACGA AACTGGACCA AAGAGATTCC AGAAGTGGCG GCTAAACGAT
    GTGGAGAAAG ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_496184.3
    CDS1..9855
    Translation

    Target ORF information:

    RefSeq Version NM_063783.3
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Location of vulva defective 1 (lov-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_063783.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGAAGAAGT CAAACTTCTT TGTTCTATTG CTACTGGCAA TATCGGCGAT ACAAATTGAT 
    GGATTGCATT ACCAACTTCT TGACGGGATC GCAACTTTTC GATTAGACAA CGATGACACA
    ACAATTGGAG GAGTTCCAAG GAATTCACAA GGAGTCGTTA AAATCAAATT GAGCTGCGGA
    TTGAATAGAC TTTCAGTTGA GAACAAGGTT ACGGAAGTTT CAAGTTTGGA ATTGATTCAC
    AACTGCATCC AAACAGAGAC CAGGCTTGTC GGACTCTTTT TGAACTCAAC TTGGATTACT
    TTGAATGAAG TGAATGATGA CGATGAGATC AGTATTGCTG TGGAGGCGAA ATATGAAGTT
    TGTTATGATG ACGGTATCGA CAGATGTGAT GGCTCCCTAT GGTGGCTCCA AGTCGGTGGA
    AACGAGATGG CGTTGCTGGG CTACCGAGAG AAGTGTGAGA GCGGAGAGAT CAACGAAGAG
    TATGCGAGAA GAATGTGCAA GCGACCTTAT CGTTCTGAAA AGAGCACAGC TATCAGTGAT
    AGTCAAGGAG TGTATTATGA TGGGCAGGTA CTTAAGGGTG TCAGGGCAAA ACAGTTTAGC
    ATGAGGACTT CTGGATCACC TACTTTGAGA AGAATGAAAC GAGACGCTGG TGATAATACT
    TGCGACTACA CTATTGAGTC AACTTCAACC TCGACTACAA CTCCAACTAC CACAACCGTG
    ACTAGTACCG TGACATCTAC AACAACCGTC CCAACAAGTA CAAGTACGGT GACTACCGCA
    ATGAGCACAA GCACATCAAC TCCATCAACA TCCACCACCA TCGAATCCAC GTCAACAACG
    TTCACTTCCA CAGCATCCAC GTCGACATCA TCAACTAGCA CAACTCAGCA GAGTTCTAGC
    ACAATCACAA GCTCGCCGAG CTCCACCACA CTGAGTACAA GTATTCCAAC CACTACAACA
    CCCGAAATCA CATCAACTTT GTCGTCTCTC CCGGATAATG CGATTTGCTC CTACCTTGAC
    GAGACCACTA CGTCTACCAC TTTCACCACC ACTATGTTGA CATCCACTAC TACCGAAGAA
    CCATCTACTT CCACAACCAC AACAGAAGTT ACATCTACAT CTTCTACTGT TACAACAACT
    GAACCAACCA CTACTTTGAC CACCTCGACT GCTTCAACGT CAACAACCGA ACCATCGACG
    AGTACTGTAA CTACTTCCCC GTCAACATCC CCTGTGACTT CTACGGTGAC ATCTAGTTCA
    AGCTCCTCGA CTACTGTAAC GACCCCAACA AGCACTGAGT CCACATCGAC ATCTCCTTCG
    TCTACAGTAA CAACCAGCAC AACTGCTCCT TCAACATCAA CTACTGGGCC ATCGAGTTCT
    TCTTCCACTC CAAGTTCAAC GGCTTCTTCG TCTGTGTCAA GCACCGCTTC TTCTACTCAA
    TCATCCACGT CTACTCAGCA AAGTTCAACA ACAACAAAAT CTGAGACAAC TACTAGCTCG
    GACGGAACTA ATCCAGACTT CTATTTTGTA GAAAAGGCAA CTACAACGTT CTATGATTCC
    ACAAGTGTGA ACCTAACGCT TAATTCCGGC CTCGGAATCA TTGGATATCA AACATCCATC
    GAATGTACAA GCCCTACATC TTCTAATTAT GTTTCAACCA CTAAAGACGG AGCATGTTTC
    ACGAAAAGTG TATCAATGCC ACGTCTCGGA GGGACGTACC CTGCTAGCAC ATTCGTTGGT
    CCTGGAAACT ACACCTTCAG AGCAACTATG ACAACCGACG ATAAAAAAGT TTACTACACG
    TATGCGAACG TTTATATTCA GGAATATTCT TCCACCACCA TAGAGTCGGA ATCTTCTACA
    TCTGCTGTAG CAAGCTCTAC GTCATCTACA CCATCAACTC CATCTTCCAC ATTATCAACA
    TCCACAGTCA CAGAGCCGTC ATCCACACGT TCAAGCGATT CTACGACAAC GTCGGCTGGG
    AGCACAACTA CTTTACAAGA ATCCACAACA ACTTCCGAAG AAAGTACTAC TGATTCCAGT
    ACAACTACCA TCTCGGACAC ATCCACAACT TCTTCCAGTC CGAGTTCTAC CACTGCTGAT
    TCAACTTCCA CTTTGTCAGT CGATCAGTTC GATTTCATCT TGGACAGTGG ATTGTCATGG
    AACGAGACAC GTCACAATGA AGACAGCATC AACATTGTAC CTTTACCAAC TAATGCCATT
    ACTCCTACTG AGAGAAGCCA AACATTTGAA TGCAGAAATG TGTCTACTGA GCCTTTTCTT
    ATAATTAAGG AATCCACTTG CTTAAACTAC TCAAACACGG TCCTAAACGC AACTTATTCT
    TCTAATATTC CAATTCAACC AATTGAAACA TTTCTTGTTG GCATTGGCAC CTATGAGTTT
    CGAATCAACA TGACGGATTT GACGACAATG CAAGTGGTCA GCCACATTTT CACATTGAAT
    GTTGTTGCGG ATTCAACAAG TACATCCGAA GTTACCAGTA CAACTTCAAC TGGATCTTCT
    TCCGAAAGTT CAGCCATTTC CACAACTTCA GGAATTGAAT CTACGTCGAC TTTGGAAGCT
    AGCACGACTG ATGCTTCACA AGACAGTTCT ACATCAACAT CGGATTCTGG CACAACTTCA
    GATTCCACCA CCATAGACTC ATCTAACTCA ACTCCATCCA CTTCAGATTC ATCTGGTTTA
    TCTCAAACAC CATCCGATTC ATCAAGTGCA TCTGATAGCA TGAGAACAAC AACTGTGGAT
    CCAGACGCAA GTACAGAAAC CCCTTACGAC TTTGTATTGG AAAATTTGAC ATGGAACGAG
    ACAGTGTATT ACTCTGAAAA TCCATTTTAT ATTACACCAA TTCCAAACAA AGAGCCCGGA
    GCTTTAACGA CTGCTATGAC CTGTCAATGT CGAAACGATT CTTCTCAGCC ATTTGTTTTA
    CTGAAGGAAA GCAATTGCTT GACAGAGTTT GGAAAAAATG GTGCATATAG TGCCTCCGTA
    TCTTTCAACC CAATGACCAG CTTTGTACCT GCAACTGGAA CTTATGAATT CTTAATTAAT
    GTTACCAATC GTGCATCTGG AGAATCTGCC AGTCACATCT TTACTATGAA TGTAGTTCTT
    CCAACTACAA CTACTGAAAC CCCACCAACC ACTGTCAGCT CGTCGGATGA TGCCGGTGGC
    AAAACCGGTG GAACTGGAGC TACTGGGGGA ACTGGTGGAA CAGGTTCAGG AGGATCTGCG
    ACAACTCTTA GCACGGGTGA TGCAGTCAGA TCAACCACTT CTGGATCTGG AAGTGGACAA
    TCATCAACGG GATCGGGGGC TGGTGGATCA GGAACGACCG CTTCTGGAAG TGGGTCAGGG
    GGGTCTTCAG GGACGGGTTC GGACGGGGTA AACTCTGGAA AAACAACGGC ATTGAATGGA
    GATGGTACAG GATCTGGAAC AGCTACCACA CCAGGATCCC ATTTAGGAGA CGGTGGTAGT
    ACATCTGGCT CAGGATCAGA TTCAAATGGA TCTTCTGGTG TTTCAACCAA GAGCTCATCT
    GGCTCCGATA CGTCTGGATC TTCCGATTCC TCTGGTGCAA ATGGTGCATT CTCTGCTACC
    GCGCAACCAT CTACAAGAAC TACTAAAACT CGCAGCTCTC TTGCAACAGT TTCTCCAATT
    TCTGCAGCTG AACAAGCGAT CATAGATGCT CAAAAAGCTG ATGTAATGAA CCAACTGGCT
    GGGATAATGG ATGGATCTGC ATCCAACAAC TCTCTAAACA CAAGCAGCTC TCTTCTCAAT
    CAAATCTCAT CTCTACCTGC CGCAGATCTA GTGGAAGTTG CTCAAAGTTT GCTCTCAAAT
    ACCCTCAAAA TCCCTGGAGT CGGAAATATG TCAAGTGTTG ATGTTTTGAA AACATTGCAA
    GACAATATTG CAACAACAAA TTCGGAATTG GCCGACGAAA TGGCAAAAGT TATCACTAAA
    CTAGCAAATG TCAATATGAC TTCCGCACAA TCCTTGAACT CCGTACTGTC TTCTCTCGAT
    CTTGCTCTCA AAGGAAGTAC AGTCTATACC TTGGGAGTAT CAAGTACAAA ATCAAAAGAT
    GGAACTTACG CGGTTATTTT TGGATATGTT ATTGCATCGG GATATACATT GGTATCGCCT
    AGGTGTACGC TCAGCATTTA CGGATCTACA ATATACCTTA CAGGAGATAC CCGGGCATCT
    TATAAACAGC TTGATGGAGA TACCGTTACT GCGGACACTA TGCTAGCAGC TGCCATCGGA
    ATCCAAGGAA TGTTCGCAAC AAATGGAAGG ACTGTGCAAG TTGAGCAGGA TAAAATAGAT
    GACAAACGAT CACTGGTTTC TGGAAATATA ATGGCTACAA TGAGTGGAGT TGGAGATGTT
    CAATCAGGGG AATATAGTTA TAATGATATG TATGTAACTG CTTGGAATGT CACTTATGAC
    AATTCAACAG TAGGATCCAC AAGTCAGAAG AACACTTCAT TTTCGTTCAA TATCCCAGTC
    TCAGAAGTTC AATACATATT ATTGATTGAA TCTGGAACAA TGATAAAACT GCATTCTACT
    CAAAATATTG TATCTAGAGG ACTAGTAGTA ACTGCATCAT ATGGTGGAGT TACTTACACA
    ATAACTTGCA CGAATGGAAC AGGAAAGTTT GTTGAAGTAG ACACAGATAA TGCCATTTTC
    TCATACAATG CTGACAGCTT CACGGTTGTG GCATCAGATG GATCCTCAGC GAGCACGGTT
    AAGAAGTTGA TTCAGATGCC GATAGTTATT GAGAACGTCA ACCTTGCTTT ATTCAATCAA
    ACTACTTCTC CACTTGTGTT CTCAAACGCG GGAAGTTATT CAATGCGAAT GGTGCTGTCT
    CCGCAGGACA TTGGAATTCC AGCTGTCTCT GCTTTATCTC AAACTGTCTC AATATCTACT
    CTTTCACCGA CGGCTTCGTA CACCAAAGAT GATTTGCAAT CATTGATTAA GGAACAAACA
    CTTGTCACCG TTTCGGGAAC TCTATTCTTC TCCAAAGCGT CATCCATCGA TGTCAGCGGC
    TACTCATTTT TCGTAGACTC CACCGCCCTT TATTTGTCAA ACTCTGTGAC AACTCTGGTG
    ATCAGTTCTC CCACCTACAA TATTGTTTCT TTGGCGCTTG GAGGTTACGG TATTCAGATA
    ACAGCCGGAA CTTACACATC TGGTTCTCAA ACTCATACGG TAACTTTAAT GGAATTCTCT
    GACACACAGA AAATGAGAAT CGATGGAGGG TTGATTATTA GAAATGGAAC GAATGGATAT
    GTAATTCAGA ATGGACAAGT TTCGACGGAA GGCGATGTTT CGGGAACTAA AATTGATATT
    GTGCCGCAAA GTTTGATGAA TCAAGAATCC CAGCAACAAA TTGAAACGAT TCTTAGCAAT
    ACTCAAGATT TTCTTACAAA TAATGGAATG ACTATGACAG ATGCAGAGAT TAATGATACA
    TCCAACTCTC TCCTTTCAAT TGCGGGATCT TTAACATCAG CGTTGAAGAT TGCTCTCGAC
    AATCCTCTTT CTTCTGATCT AGCTGCCAAT TTGAAATATG CAACAGATAA CTATGATTCC
    TTGTACAACG TGCTACCAAG TGATCCAGAC AATATTGTGT ACGTGGAGGA AATGACGTCA
    GAAGAATGGG CTGCTTACGT CACAAAAATG TTCCAGAAGA ACATCGCCAA AAATCTAGCC
    AACCAACTTG CATCTACACT AGATACCCTG GAAAACACGC TAGCAGCTAG AGCTATAGCA
    ACTGGCAACT TACCTTATGA CTACTCGAAT AGCGTCGACG GAACCGGAAT GGTAATCGTT
    ATCGATGATG CATCAAATAT TGTAGGAAAA ACTCAAAACT GTGAAGAATG GGCATTCAAA
    CTTCCATCGC CCGCTTCAAC TCTGAATACT GCTGAGATCA CAGATAAAAC ACTGATTCAA
    GTTGGATTAG TTTGCTATGC CACAAACCCG AGAACTTATG TGGATAACTT TGATATGTTA
    ATTACCTCAG GTGCACTTGA AGCACATATC AAAGATGAGA ATCAGATAAT CATTCCAATA
    ACCGGAACTA CTGCTCCAAT TTATGTGAAC GGCAGGGGAA GTGAGGATGA TGCAGTGCTA
    ACTTTAATGC AACAAGGAGA CTTTGCCAGT TATCAGATAT TAGATCTTCA TGCTTTTCGA
    ACCACTAACT GGAACAACTC GCTTCAAGTA GAGATTATTG CATCTCAAGA CTATGAAATT
    CCAAACAACG ATGACACCTA CATGTTCTCA TCATTCCAAA GTCTTCCTGG CCCACTTGAA
    TCAAATCATG AATGGATCTT TGACTTGAAC ACATTGAACA AGACAAGCAA TTATTTTGTC
    ACCGCCGGAA ATTTGATCAA CAACACTGGA CTTTTCTTTA TTGGAATTGG AAAAAGAAAT
    AGCTCTACTA ACACTGGAAA TAGTTCGGAT ATTGTAAACT ATGGTCAGTA CGATTCAATG
    CAGTGGTCAT TTGCACGAAG TGTACCAATG GATTATCAAG TCGCTGCAGT GAGTAAAGGA
    TGTTACTTCT ATCAGAAAAC ATCGGATGTC TTCAATTCTG AAGGAATGTA TCCAAGTGAT
    GGACAAGGAA TGCAGTTCGT TAATTGTTCA ACTGATCATT TAACAATGTT CTCTGTTGGT
    GCATTCAATC CAACTATTGA TGCAGATTTC TCTTACAATT ATAATGTGAA TGAAATCGAA
    AAGAATGTGA AAGTGATGAT TGCCGCTGTT TTCATGCTAG TAGTTTACGG ATGTCTTACC
    ATCAATGCAA TCATCTGTCA ACGTAAAGAT GCATCACGCG GGAGACTTCG TTTTCTGAAA
    GACAATGAGC CTCATGATGG ATACATGTAT GTGATTGCCG TGGAGACTGG ATATCGAATG
    TTTGCAACTA CGGATAGTAC GATATGTTTC AATTTATCTG GAAATGAAGG GGATCAGATT
    TTCCGATCGT TCAGATCAGA AGAAGATGGT AATTGGGAGT TTCCGTTTTC ATGGGGAACC
    ACTGATCGAT TTGTGATGAC AACGGCATTC CCTCTTGGCG AGTTGGAATA CATGAGACTC
    TGGCTTGACG ATGCTGGTCT AGATCACCGA GAGTCGTGGT ATTGCAATCG AATAATTGTG
    AAAGATCTAC AGACTCAGGA TATCTACTAC TTCCCTTTCA ACAATTGGCT TGGAACTAAA
    AACGGAGACG GAGAGACTGA AAGACTAGCG CGAGTGGAGT ATAAGCGAAG GTTTTTGGAT
    GAGTCAATGA GTATGCATAT GCTGGCTCAA ACAATTTCCT GGTTTGCCAT GTTTACTGGT
    GGAGGCAATC GGTTGAGGGA TCGAGTGTCA AGGCAAGACT ATTCTGTCAG TATCATCTTC
    TCACTTGTTG TGGTCAGCAT GATTAGCATC ACAATTCTGA AGAGCGATAA TTCGATTATC
    AGTGATTCGA AGAGTGTCTC CGAGTTCACT TTCACTATAA AAGATATTGC ATTCGGAGTT
    GGATTCGGTG TTCTTATCAC TTTCCTCAAC TCATTGCACA TTTTATTATG CACCAAATGT
    CGTTCCCACA GTGAACATTA CTACTACAAA AAACGAAAGA GAGAGGATCC GGAATTCAAG
    GATAATAGTG GTTCATGGCC GATGTTCATG GCTGGGATGG CACGTACCAT CATAGTATTT
    CCAGTTCTCA TGGGTCTTAT TTATATTTCT GGAGCTGGAA TGAGCTTGAT GGATGATTTA
    GCCAACTCTT TCTATATCCG ATTTCTGATC AGTCTGATTC TATGGGCAGT GGTGTTCGAG
    CCAATTAAGG GATTGATTTG GGCGTTTTTA ATTTTGAAAA CCAGAAAGAG TCATAAAATC
    ATTAACAAGT TGGAGGAAGC ATTGCTAAGA GCTAAGCCAG CTGAGACGTT CTTGAGAAAT
    CCCTATGGGA AAATCGAAAA AGGTCTGGGA ACCGAAATTG CTGATGTTAC AAAGCTCCGA
    GACACTGAAA ACCGAAAAAT GAGAGATGAA CAGTTATTTA TAACCATTCG TGATATGCTT
    TGTTTCTTTG CTTCTTTGTA TATTATGGTT ATGCTGACAT ACTATTGCAA AGACCGGCAT
    GGATATTGGT ATCAGTTGGA GATGAGCACA ATCTTGAACA TCAATCAAAA GAACTACGGT
    GACAATACTT TCATGTCAAT CCAACATGCT GACGATTTCT GGGATTGGGC TCGTGAATCT
    CTGGCCACTG CTCTTTTGGC TTCTTGGTAC GACGGAAATC CTGCATACGG GATGAGAGCC
    TACATGAATG ATAAAGTTTC AAGAAGTATG GGAATAGGTA CGATACGGCA GGTTCGGACA
    AAGAAAAGTG CAGAGTGTAC GATGTTCAAG CAGTTTCAAG GATACATCAA TGATTGTGGA
    GAGGAGTTGA CAAGCAAAAA CGAAGAAAAA ACTTTGTACA TGCAAGCTGG TTGGACCGAA
    CTGGAATCCG AGAATGGAAC AGATGCTTCA GATGAATACA CCTACAAGAC TTCTGAAGAA
    TTATCCACTG AAACAGTTTC AGGCCTACTG TATAGCTATT CAGGAGGTGG ATATACTATC
    AGTATGTCTG GTACCCAAGC GGAAATCATC ACTCTCTTCA ATAAACTTGA TTCGGAACGA
    TGGATCGATG ATCATACTCG AGCAGTCATC ATTGAGTTCT CTGCATACAA TGCTCAAATC
    AACTACTTTT CGGTGGTACA GTTGTTGGTG GAGATTCCAA AGTCAGGAAT TTATCTTCCT
    AATAGTTGGG TTGAATCAGT AAGACTTATC AAATCAGAGG GAAGCGATGG AACTGTGGTC
    AAGTATTATG AGATGCTTTA CATCTTTTTC TCAGTTCTCA TATTTGTAAA AGAGATTGTG
    TTCTACTTGT ATGGGCGGTA CAAGGTGATA ACAACAATGA AACCAACGAG AAATCCATTC
    AAAATTGTGT ATCAGCTGGC ACTGGGAAAC TTCTCACCAT GGAACTTCAT GGACTTGATA
    GTTGGAGCTC TCGCTGTTGC ATCGGTCCTA GCCTACACCA TCCGCCAACG AACCACAAAT
    AGAGCAATGG AAGATTTCAA TGCAAACAAT GGAAATTCGT ACATTAATTT GACTGAACAG
    AGAAATTGGG AGATTGTATT CAGTTATTGT CTTGCCGGAG CCGTATTCTT CACTTCTTGT
    AAAATGATTC GAATCTTGAG ATTCAACAGA AGAATTGGAG TATTAGCGGC TACACTGGAT
    AACGCTCTCG GAGCTATTGT GTCATTTGGA ATTGCTTTCC TGTTCTTCAG CATGACATTC
    AACTCTGTAT TGTACGCAGT TTTAGGAAAT AAAATGGGAG GATATCGGAG CTTAATGGCT
    ACCTTTCAAA CAGCATTGGC TGGAATGCTT GGAAAACTTG ATGTTACCTC TATTCAACCG
    ATTTCTCAAT TTGCGTTTGT TGTGATCATG CTTTATATGA TTGCCGGATC AAAGCTCGTC
    CTGCAACTTT ACGTCACTAT AATTATGTTC GAGTTCGAGG AAATTCGTAA TGACAGTGAG
    AAGCAGACCA ATGATTACGA AATAATCGAT CACATAAAAT ACAAGACAAA ACGTCGCTTG
    GGTCTTCTTG AACCAAAAGA TTTTGCTCCA GTTTCAATTG CTGACACTCA AAAAGACTTC
    CGTCTATTCC ACAGCGCTGT TGCCAAAGTC AATCTGCTTC ATCATCGTGC CACCAGAATG
    CTCCAAACTC AAGGTCAATA CCAAAACCAA ACAGTCATCA ACTACACACT CTCCTATGAT
    CCTGTCTCTG CTATTCACGA AACTGGACCA AAGAGATTCC AGAAGTGGCG GCTAAACGAT
    GTGGAGAAAG ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa337623
    Clone ID Related Accession (Same CDS sequence) NM_063783.3 , NM_063783.4
    Accession Version NM_063783.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Location of vulva defective 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_063783.3. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGAAGAAGT CAAACTTCTT TGTTCTATTG CTACTGGCAA TATCGGCGAT ACAAATTGAT 
    GGATTGCATT ACCAACTTCT TGACGGGATC GCAACTTTTC GATTAGACAA CGATGACACA
    ACAATTGGAG GAGTTCCAAG GAATTCACAA GGAGTCGTTA AAATCAAATT GAGCTGCGGA
    TTGAATAGAC TTTCAGTTGA GAACAAGGTT ACGGAAGTTT CAAGTTTGGA ATTGATTCAC
    AACTGCATCC AAACAGAGAC CAGGCTTGTC GGACTCTTTT TGAACTCAAC TTGGATTACT
    TTGAATGAAG TGAATGATGA CGATGAGATC AGTATTGCTG TGGAGGCGAA ATATGAAGTT
    TGTTATGATG ACGGTATCGA CAGATGTGAT GGCTCCCTAT GGTGGCTCCA AGTCGGTGGA
    AACGAGATGG CGTTGCTGGG CTACCGAGAG AAGTGTGAGA GCGGAGAGAT CAACGAAGAG
    TATGCGAGAA GAATGTGCAA GCGACCTTAT CGTTCTGAAA AGAGCACAGC TATCAGTGAT
    AGTCAAGGAG TGTATTATGA TGGGCAGGTA CTTAAGGGTG TCAGGGCAAA ACAGTTTAGC
    ATGAGGACTT CTGGATCACC TACTTTGAGA AGAATGAAAC GAGACGCTGG TGATAATACT
    TGCGACTACA CTATTGAGTC AACTTCAACC TCGACTACAA CTCCAACTAC CACAACCGTG
    ACTAGTACCG TGACATCTAC AACAACCGTC CCAACAAGTA CAAGTACGGT GACTACCGCA
    ATGAGCACAA GCACATCAAC TCCATCAACA TCCACCACCA TCGAATCCAC GTCAACAACG
    TTCACTTCCA CAGCATCCAC GTCGACATCA TCAACTAGCA CAACTCAGCA GAGTTCTAGC
    ACAATCACAA GCTCGCCGAG CTCCACCACA CTGAGTACAA GTATTCCAAC CACTACAACA
    CCCGAAATCA CATCAACTTT GTCGTCTCTC CCGGATAATG CGATTTGCTC CTACCTTGAC
    GAGACCACTA CGTCTACCAC TTTCACCACC ACTATGTTGA CATCCACTAC TACCGAAGAA
    CCATCTACTT CCACAACCAC AACAGAAGTT ACATCTACAT CTTCTACTGT TACAACAACT
    GAACCAACCA CTACTTTGAC CACCTCGACT GCTTCAACGT CAACAACCGA ACCATCGACG
    AGTACTGTAA CTACTTCCCC GTCAACATCC CCTGTGACTT CTACGGTGAC ATCTAGTTCA
    AGCTCCTCGA CTACTGTAAC GACCCCAACA AGCACTGAGT CCACATCGAC ATCTCCTTCG
    TCTACAGTAA CAACCAGCAC AACTGCTCCT TCAACATCAA CTACTGGGCC ATCGAGTTCT
    TCTTCCACTC CAAGTTCAAC GGCTTCTTCG TCTGTGTCAA GCACCGCTTC TTCTACTCAA
    TCATCCACGT CTACTCAGCA AAGTTCAACA ACAACAAAAT CTGAGACAAC TACTAGCTCG
    GACGGAACTA ATCCAGACTT CTATTTTGTA GAAAAGGCAA CTACAACGTT CTATGATTCC
    ACAAGTGTGA ACCTAACGCT TAATTCCGGC CTCGGAATCA TTGGATATCA AACATCCATC
    GAATGTACAA GCCCTACATC TTCTAATTAT GTTTCAACCA CTAAAGACGG AGCATGTTTC
    ACGAAAAGTG TATCAATGCC ACGTCTCGGA GGGACGTACC CTGCTAGCAC ATTCGTTGGT
    CCTGGAAACT ACACCTTCAG AGCAACTATG ACAACCGACG ATAAAAAAGT TTACTACACG
    TATGCGAACG TTTATATTCA GGAATATTCT TCCACCACCA TAGAGTCGGA ATCTTCTACA
    TCTGCTGTAG CAAGCTCTAC GTCATCTACA CCATCAACTC CATCTTCCAC ATTATCAACA
    TCCACAGTCA CAGAGCCGTC ATCCACACGT TCAAGCGATT CTACGACAAC GTCGGCTGGG
    AGCACAACTA CTTTACAAGA ATCCACAACA ACTTCCGAAG AAAGTACTAC TGATTCCAGT
    ACAACTACCA TCTCGGACAC ATCCACAACT TCTTCCAGTC CGAGTTCTAC CACTGCTGAT
    TCAACTTCCA CTTTGTCAGT CGATCAGTTC GATTTCATCT TGGACAGTGG ATTGTCATGG
    AACGAGACAC GTCACAATGA AGACAGCATC AACATTGTAC CTTTACCAAC TAATGCCATT
    ACTCCTACTG AGAGAAGCCA AACATTTGAA TGCAGAAATG TGTCTACTGA GCCTTTTCTT
    ATAATTAAGG AATCCACTTG CTTAAACTAC TCAAACACGG TCCTAAACGC AACTTATTCT
    TCTAATATTC CAATTCAACC AATTGAAACA TTTCTTGTTG GCATTGGCAC CTATGAGTTT
    CGAATCAACA TGACGGATTT GACGACAATG CAAGTGGTCA GCCACATTTT CACATTGAAT
    GTTGTTGCGG ATTCAACAAG TACATCCGAA GTTACCAGTA CAACTTCAAC TGGATCTTCT
    TCCGAAAGTT CAGCCATTTC CACAACTTCA GGAATTGAAT CTACGTCGAC TTTGGAAGCT
    AGCACGACTG ATGCTTCACA AGACAGTTCT ACATCAACAT CGGATTCTGG CACAACTTCA
    GATTCCACCA CCATAGACTC ATCTAACTCA ACTCCATCCA CTTCAGATTC ATCTGGTTTA
    TCTCAAACAC CATCCGATTC ATCAAGTGCA TCTGATAGCA TGAGAACAAC AACTGTGGAT
    CCAGACGCAA GTACAGAAAC CCCTTACGAC TTTGTATTGG AAAATTTGAC ATGGAACGAG
    ACAGTGTATT ACTCTGAAAA TCCATTTTAT ATTACACCAA TTCCAAACAA AGAGCCCGGA
    GCTTTAACGA CTGCTATGAC CTGTCAATGT CGAAACGATT CTTCTCAGCC ATTTGTTTTA
    CTGAAGGAAA GCAATTGCTT GACAGAGTTT GGAAAAAATG GTGCATATAG TGCCTCCGTA
    TCTTTCAACC CAATGACCAG CTTTGTACCT GCAACTGGAA CTTATGAATT CTTAATTAAT
    GTTACCAATC GTGCATCTGG AGAATCTGCC AGTCACATCT TTACTATGAA TGTAGTTCTT
    CCAACTACAA CTACTGAAAC CCCACCAACC ACTGTCAGCT CGTCGGATGA TGCCGGTGGC
    AAAACCGGTG GAACTGGAGC TACTGGGGGA ACTGGTGGAA CAGGTTCAGG AGGATCTGCG
    ACAACTCTTA GCACGGGTGA TGCAGTCAGA TCAACCACTT CTGGATCTGG AAGTGGACAA
    TCATCAACGG GATCGGGGGC TGGTGGATCA GGAACGACCG CTTCTGGAAG TGGGTCAGGG
    GGGTCTTCAG GGACGGGTTC GGACGGGGTA AACTCTGGAA AAACAACGGC ATTGAATGGA
    GATGGTACAG GATCTGGAAC AGCTACCACA CCAGGATCCC ATTTAGGAGA CGGTGGTAGT
    ACATCTGGCT CAGGATCAGA TTCAAATGGA TCTTCTGGTG TTTCAACCAA GAGCTCATCT
    GGCTCCGATA CGTCTGGATC TTCCGATTCC TCTGGTGCAA ATGGTGCATT CTCTGCTACC
    GCGCAACCAT CTACAAGAAC TACTAAAACT CGCAGCTCTC TTGCAACAGT TTCTCCAATT
    TCTGCAGCTG AACAAGCGAT CATAGATGCT CAAAAAGCTG ATGTAATGAA CCAACTGGCT
    GGGATAATGG ATGGATCTGC ATCCAACAAC TCTCTAAACA CAAGCAGCTC TCTTCTCAAT
    CAAATCTCAT CTCTACCTGC CGCAGATCTA GTGGAAGTTG CTCAAAGTTT GCTCTCAAAT
    ACCCTCAAAA TCCCTGGAGT CGGAAATATG TCAAGTGTTG ATGTTTTGAA AACATTGCAA
    GACAATATTG CAACAACAAA TTCGGAATTG GCCGACGAAA TGGCAAAAGT TATCACTAAA
    CTAGCAAATG TCAATATGAC TTCCGCACAA TCCTTGAACT CCGTACTGTC TTCTCTCGAT
    CTTGCTCTCA AAGGAAGTAC AGTCTATACC TTGGGAGTAT CAAGTACAAA ATCAAAAGAT
    GGAACTTACG CGGTTATTTT TGGATATGTT ATTGCATCGG GATATACATT GGTATCGCCT
    AGGTGTACGC TCAGCATTTA CGGATCTACA ATATACCTTA CAGGAGATAC CCGGGCATCT
    TATAAACAGC TTGATGGAGA TACCGTTACT GCGGACACTA TGCTAGCAGC TGCCATCGGA
    ATCCAAGGAA TGTTCGCAAC AAATGGAAGG ACTGTGCAAG TTGAGCAGGA TAAAATAGAT
    GACAAACGAT CACTGGTTTC TGGAAATATA ATGGCTACAA TGAGTGGAGT TGGAGATGTT
    CAATCAGGGG AATATAGTTA TAATGATATG TATGTAACTG CTTGGAATGT CACTTATGAC
    AATTCAACAG TAGGATCCAC AAGTCAGAAG AACACTTCAT TTTCGTTCAA TATCCCAGTC
    TCAGAAGTTC AATACATATT ATTGATTGAA TCTGGAACAA TGATAAAACT GCATTCTACT
    CAAAATATTG TATCTAGAGG ACTAGTAGTA ACTGCATCAT ATGGTGGAGT TACTTACACA
    ATAACTTGCA CGAATGGAAC AGGAAAGTTT GTTGAAGTAG ACACAGATAA TGCCATTTTC
    TCATACAATG CTGACAGCTT CACGGTTGTG GCATCAGATG GATCCTCAGC GAGCACGGTT
    AAGAAGTTGA TTCAGATGCC GATAGTTATT GAGAACGTCA ACCTTGCTTT ATTCAATCAA
    ACTACTTCTC CACTTGTGTT CTCAAACGCG GGAAGTTATT CAATGCGAAT GGTGCTGTCT
    CCGCAGGACA TTGGAATTCC AGCTGTCTCT GCTTTATCTC AAACTGTCTC AATATCTACT
    CTTTCACCGA CGGCTTCGTA CACCAAAGAT GATTTGCAAT CATTGATTAA GGAACAAACA
    CTTGTCACCG TTTCGGGAAC TCTATTCTTC TCCAAAGCGT CATCCATCGA TGTCAGCGGC
    TACTCATTTT TCGTAGACTC CACCGCCCTT TATTTGTCAA ACTCTGTGAC AACTCTGGTG
    ATCAGTTCTC CCACCTACAA TATTGTTTCT TTGGCGCTTG GAGGTTACGG TATTCAGATA
    ACAGCCGGAA CTTACACATC TGGTTCTCAA ACTCATACGG TAACTTTAAT GGAATTCTCT
    GACACACAGA AAATGAGAAT CGATGGAGGG TTGATTATTA GAAATGGAAC GAATGGATAT
    GTAATTCAGA ATGGACAAGT TTCGACGGAA GGCGATGTTT CGGGAACTAA AATTGATATT
    GTGCCGCAAA GTTTGATGAA TCAAGAATCC CAGCAACAAA TTGAAACGAT TCTTAGCAAT
    ACTCAAGATT TTCTTACAAA TAATGGAATG ACTATGACAG ATGCAGAGAT TAATGATACA
    TCCAACTCTC TCCTTTCAAT TGCGGGATCT TTAACATCAG CGTTGAAGAT TGCTCTCGAC
    AATCCTCTTT CTTCTGATCT AGCTGCCAAT TTGAAATATG CAACAGATAA CTATGATTCC
    TTGTACAACG TGCTACCAAG TGATCCAGAC AATATTGTGT ACGTGGAGGA AATGACGTCA
    GAAGAATGGG CTGCTTACGT CACAAAAATG TTCCAGAAGA ACATCGCCAA AAATCTAGCC
    AACCAACTTG CATCTACACT AGATACCCTG GAAAACACGC TAGCAGCTAG AGCTATAGCA
    ACTGGCAACT TACCTTATGA CTACTCGAAT AGCGTCGACG GAACCGGAAT GGTAATCGTT
    ATCGATGATG CATCAAATAT TGTAGGAAAA ACTCAAAACT GTGAAGAATG GGCATTCAAA
    CTTCCATCGC CCGCTTCAAC TCTGAATACT GCTGAGATCA CAGATAAAAC ACTGATTCAA
    GTTGGATTAG TTTGCTATGC CACAAACCCG AGAACTTATG TGGATAACTT TGATATGTTA
    ATTACCTCAG GTGCACTTGA AGCACATATC AAAGATGAGA ATCAGATAAT CATTCCAATA
    ACCGGAACTA CTGCTCCAAT TTATGTGAAC GGCAGGGGAA GTGAGGATGA TGCAGTGCTA
    ACTTTAATGC AACAAGGAGA CTTTGCCAGT TATCAGATAT TAGATCTTCA TGCTTTTCGA
    ACCACTAACT GGAACAACTC GCTTCAAGTA GAGATTATTG CATCTCAAGA CTATGAAATT
    CCAAACAACG ATGACACCTA CATGTTCTCA TCATTCCAAA GTCTTCCTGG CCCACTTGAA
    TCAAATCATG AATGGATCTT TGACTTGAAC ACATTGAACA AGACAAGCAA TTATTTTGTC
    ACCGCCGGAA ATTTGATCAA CAACACTGGA CTTTTCTTTA TTGGAATTGG AAAAAGAAAT
    AGCTCTACTA ACACTGGAAA TAGTTCGGAT ATTGTAAACT ATGGTCAGTA CGATTCAATG
    CAGTGGTCAT TTGCACGAAG TGTACCAATG GATTATCAAG TCGCTGCAGT GAGTAAAGGA
    TGTTACTTCT ATCAGAAAAC ATCGGATGTC TTCAATTCTG AAGGAATGTA TCCAAGTGAT
    GGACAAGGAA TGCAGTTCGT TAATTGTTCA ACTGATCATT TAACAATGTT CTCTGTTGGT
    GCATTCAATC CAACTATTGA TGCAGATTTC TCTTACAATT ATAATGTGAA TGAAATCGAA
    AAGAATGTGA AAGTGATGAT TGCCGCTGTT TTCATGCTAG TAGTTTACGG ATGTCTTACC
    ATCAATGCAA TCATCTGTCA ACGTAAAGAT GCATCACGCG GGAGACTTCG TTTTCTGAAA
    GACAATGAGC CTCATGATGG ATACATGTAT GTGATTGCCG TGGAGACTGG ATATCGAATG
    TTTGCAACTA CGGATAGTAC GATATGTTTC AATTTATCTG GAAATGAAGG GGATCAGATT
    TTCCGATCGT TCAGATCAGA AGAAGATGGT AATTGGGAGT TTCCGTTTTC ATGGGGAACC
    ACTGATCGAT TTGTGATGAC AACGGCATTC CCTCTTGGCG AGTTGGAATA CATGAGACTC
    TGGCTTGACG ATGCTGGTCT AGATCACCGA GAGTCGTGGT ATTGCAATCG AATAATTGTG
    AAAGATCTAC AGACTCAGGA TATCTACTAC TTCCCTTTCA ACAATTGGCT TGGAACTAAA
    AACGGAGACG GAGAGACTGA AAGACTAGCG CGAGTGGAGT ATAAGCGAAG GTTTTTGGAT
    GAGTCAATGA GTATGCATAT GCTGGCTCAA ACAATTTCCT GGTTTGCCAT GTTTACTGGT
    GGAGGCAATC GGTTGAGGGA TCGAGTGTCA AGGCAAGACT ATTCTGTCAG TATCATCTTC
    TCACTTGTTG TGGTCAGCAT GATTAGCATC ACAATTCTGA AGAGCGATAA TTCGATTATC
    AGTGATTCGA AGAGTGTCTC CGAGTTCACT TTCACTATAA AAGATATTGC ATTCGGAGTT
    GGATTCGGTG TTCTTATCAC TTTCCTCAAC TCATTGCACA TTTTATTATG CACCAAATGT
    CGTTCCCACA GTGAACATTA CTACTACAAA AAACGAAAGA GAGAGGATCC GGAATTCAAG
    GATAATAGTG GTTCATGGCC GATGTTCATG GCTGGGATGG CACGTACCAT CATAGTATTT
    CCAGTTCTCA TGGGTCTTAT TTATATTTCT GGAGCTGGAA TGAGCTTGAT GGATGATTTA
    GCCAACTCTT TCTATATCCG ATTTCTGATC AGTCTGATTC TATGGGCAGT GGTGTTCGAG
    CCAATTAAGG GATTGATTTG GGCGTTTTTA ATTTTGAAAA CCAGAAAGAG TCATAAAATC
    ATTAACAAGT TGGAGGAAGC ATTGCTAAGA GCTAAGCCAG CTGAGACGTT CTTGAGAAAT
    CCCTATGGGA AAATCGAAAA AGGTCTGGGA ACCGAAATTG CTGATGTTAC AAAGCTCCGA
    GACACTGAAA ACCGAAAAAT GAGAGATGAA CAGTTATTTA TAACCATTCG TGATATGCTT
    TGTTTCTTTG CTTCTTTGTA TATTATGGTT ATGCTGACAT ACTATTGCAA AGACCGGCAT
    GGATATTGGT ATCAGTTGGA GATGAGCACA ATCTTGAACA TCAATCAAAA GAACTACGGT
    GACAATACTT TCATGTCAAT CCAACATGCT GACGATTTCT GGGATTGGGC TCGTGAATCT
    CTGGCCACTG CTCTTTTGGC TTCTTGGTAC GACGGAAATC CTGCATACGG GATGAGAGCC
    TACATGAATG ATAAAGTTTC AAGAAGTATG GGAATAGGTA CGATACGGCA GGTTCGGACA
    AAGAAAAGTG CAGAGTGTAC GATGTTCAAG CAGTTTCAAG GATACATCAA TGATTGTGGA
    GAGGAGTTGA CAAGCAAAAA CGAAGAAAAA ACTTTGTACA TGCAAGCTGG TTGGACCGAA
    CTGGAATCCG AGAATGGAAC AGATGCTTCA GATGAATACA CCTACAAGAC TTCTGAAGAA
    TTATCCACTG AAACAGTTTC AGGCCTACTG TATAGCTATT CAGGAGGTGG ATATACTATC
    AGTATGTCTG GTACCCAAGC GGAAATCATC ACTCTCTTCA ATAAACTTGA TTCGGAACGA
    TGGATCGATG ATCATACTCG AGCAGTCATC ATTGAGTTCT CTGCATACAA TGCTCAAATC
    AACTACTTTT CGGTGGTACA GTTGTTGGTG GAGATTCCAA AGTCAGGAAT TTATCTTCCT
    AATAGTTGGG TTGAATCAGT AAGACTTATC AAATCAGAGG GAAGCGATGG AACTGTGGTC
    AAGTATTATG AGATGCTTTA CATCTTTTTC TCAGTTCTCA TATTTGTAAA AGAGATTGTG
    TTCTACTTGT ATGGGCGGTA CAAGGTGATA ACAACAATGA AACCAACGAG AAATCCATTC
    AAAATTGTGT ATCAGCTGGC ACTGGGAAAC TTCTCACCAT GGAACTTCAT GGACTTGATA
    GTTGGAGCTC TCGCTGTTGC ATCGGTCCTA GCCTACACCA TCCGCCAACG AACCACAAAT
    AGAGCAATGG AAGATTTCAA TGCAAACAAT GGAAATTCGT ACATTAATTT GACTGAACAG
    AGAAATTGGG AGATTGTATT CAGTTATTGT CTTGCCGGAG CCGTATTCTT CACTTCTTGT
    AAAATGATTC GAATCTTGAG ATTCAACAGA AGAATTGGAG TATTAGCGGC TACACTGGAT
    AACGCTCTCG GAGCTATTGT GTCATTTGGA ATTGCTTTCC TGTTCTTCAG CATGACATTC
    AACTCTGTAT TGTACGCAGT TTTAGGAAAT AAAATGGGAG GATATCGGAG CTTAATGGCT
    ACCTTTCAAA CAGCATTGGC TGGAATGCTT GGAAAACTTG ATGTTACCTC TATTCAACCG
    ATTTCTCAAT TTGCGTTTGT TGTGATCATG CTTTATATGA TTGCCGGATC AAAGCTCGTC
    CTGCAACTTT ACGTCACTAT AATTATGTTC GAGTTCGAGG AAATTCGTAA TGACAGTGAG
    AAGCAGACCA ATGATTACGA AATAATCGAT CACATAAAAT ACAAGACAAA ACGTCGCTTG
    GGTCTTCTTG AACCAAAAGA TTTTGCTCCA GTTTCAATTG CTGACACTCA AAAAGACTTC
    CGTCTATTCC ACAGCGCTGT TGCCAAAGTC AATCTGCTTC ATCATCGTGC CACCAGAATG
    CTCCAAACTC AAGGTCAATA CCAAAACCAA ACAGTCATCA ACTACACACT CTCCTATGAT
    CCTGTCTCTG CTATTCACGA AACTGGACCA AAGAGATTCC AGAAGTGGCG GCTAAACGAT
    GTGGAGAAAG ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_496184.3
    CDS1..9855
    Translation

    Target ORF information:

    RefSeq Version NM_063783.4
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Location of vulva defective 1 (lov-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_063783.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    ATGAAGAAGT CAAACTTCTT TGTTCTATTG CTACTGGCAA TATCGGCGAT ACAAATTGAT 
    GGATTGCATT ACCAACTTCT TGACGGGATC GCAACTTTTC GATTAGACAA CGATGACACA
    ACAATTGGAG GAGTTCCAAG GAATTCACAA GGAGTCGTTA AAATCAAATT GAGCTGCGGA
    TTGAATAGAC TTTCAGTTGA GAACAAGGTT ACGGAAGTTT CAAGTTTGGA ATTGATTCAC
    AACTGCATCC AAACAGAGAC CAGGCTTGTC GGACTCTTTT TGAACTCAAC TTGGATTACT
    TTGAATGAAG TGAATGATGA CGATGAGATC AGTATTGCTG TGGAGGCGAA ATATGAAGTT
    TGTTATGATG ACGGTATCGA CAGATGTGAT GGCTCCCTAT GGTGGCTCCA AGTCGGTGGA
    AACGAGATGG CGTTGCTGGG CTACCGAGAG AAGTGTGAGA GCGGAGAGAT CAACGAAGAG
    TATGCGAGAA GAATGTGCAA GCGACCTTAT CGTTCTGAAA AGAGCACAGC TATCAGTGAT
    AGTCAAGGAG TGTATTATGA TGGGCAGGTA CTTAAGGGTG TCAGGGCAAA ACAGTTTAGC
    ATGAGGACTT CTGGATCACC TACTTTGAGA AGAATGAAAC GAGACGCTGG TGATAATACT
    TGCGACTACA CTATTGAGTC AACTTCAACC TCGACTACAA CTCCAACTAC CACAACCGTG
    ACTAGTACCG TGACATCTAC AACAACCGTC CCAACAAGTA CAAGTACGGT GACTACCGCA
    ATGAGCACAA GCACATCAAC TCCATCAACA TCCACCACCA TCGAATCCAC GTCAACAACG
    TTCACTTCCA CAGCATCCAC GTCGACATCA TCAACTAGCA CAACTCAGCA GAGTTCTAGC
    ACAATCACAA GCTCGCCGAG CTCCACCACA CTGAGTACAA GTATTCCAAC CACTACAACA
    CCCGAAATCA CATCAACTTT GTCGTCTCTC CCGGATAATG CGATTTGCTC CTACCTTGAC
    GAGACCACTA CGTCTACCAC TTTCACCACC ACTATGTTGA CATCCACTAC TACCGAAGAA
    CCATCTACTT CCACAACCAC AACAGAAGTT ACATCTACAT CTTCTACTGT TACAACAACT
    GAACCAACCA CTACTTTGAC CACCTCGACT GCTTCAACGT CAACAACCGA ACCATCGACG
    AGTACTGTAA CTACTTCCCC GTCAACATCC CCTGTGACTT CTACGGTGAC ATCTAGTTCA
    AGCTCCTCGA CTACTGTAAC GACCCCAACA AGCACTGAGT CCACATCGAC ATCTCCTTCG
    TCTACAGTAA CAACCAGCAC AACTGCTCCT TCAACATCAA CTACTGGGCC ATCGAGTTCT
    TCTTCCACTC CAAGTTCAAC GGCTTCTTCG TCTGTGTCAA GCACCGCTTC TTCTACTCAA
    TCATCCACGT CTACTCAGCA AAGTTCAACA ACAACAAAAT CTGAGACAAC TACTAGCTCG
    GACGGAACTA ATCCAGACTT CTATTTTGTA GAAAAGGCAA CTACAACGTT CTATGATTCC
    ACAAGTGTGA ACCTAACGCT TAATTCCGGC CTCGGAATCA TTGGATATCA AACATCCATC
    GAATGTACAA GCCCTACATC TTCTAATTAT GTTTCAACCA CTAAAGACGG AGCATGTTTC
    ACGAAAAGTG TATCAATGCC ACGTCTCGGA GGGACGTACC CTGCTAGCAC ATTCGTTGGT
    CCTGGAAACT ACACCTTCAG AGCAACTATG ACAACCGACG ATAAAAAAGT TTACTACACG
    TATGCGAACG TTTATATTCA GGAATATTCT TCCACCACCA TAGAGTCGGA ATCTTCTACA
    TCTGCTGTAG CAAGCTCTAC GTCATCTACA CCATCAACTC CATCTTCCAC ATTATCAACA
    TCCACAGTCA CAGAGCCGTC ATCCACACGT TCAAGCGATT CTACGACAAC GTCGGCTGGG
    AGCACAACTA CTTTACAAGA ATCCACAACA ACTTCCGAAG AAAGTACTAC TGATTCCAGT
    ACAACTACCA TCTCGGACAC ATCCACAACT TCTTCCAGTC CGAGTTCTAC CACTGCTGAT
    TCAACTTCCA CTTTGTCAGT CGATCAGTTC GATTTCATCT TGGACAGTGG ATTGTCATGG
    AACGAGACAC GTCACAATGA AGACAGCATC AACATTGTAC CTTTACCAAC TAATGCCATT
    ACTCCTACTG AGAGAAGCCA AACATTTGAA TGCAGAAATG TGTCTACTGA GCCTTTTCTT
    ATAATTAAGG AATCCACTTG CTTAAACTAC TCAAACACGG TCCTAAACGC AACTTATTCT
    TCTAATATTC CAATTCAACC AATTGAAACA TTTCTTGTTG GCATTGGCAC CTATGAGTTT
    CGAATCAACA TGACGGATTT GACGACAATG CAAGTGGTCA GCCACATTTT CACATTGAAT
    GTTGTTGCGG ATTCAACAAG TACATCCGAA GTTACCAGTA CAACTTCAAC TGGATCTTCT
    TCCGAAAGTT CAGCCATTTC CACAACTTCA GGAATTGAAT CTACGTCGAC TTTGGAAGCT
    AGCACGACTG ATGCTTCACA AGACAGTTCT ACATCAACAT CGGATTCTGG CACAACTTCA
    GATTCCACCA CCATAGACTC ATCTAACTCA ACTCCATCCA CTTCAGATTC ATCTGGTTTA
    TCTCAAACAC CATCCGATTC ATCAAGTGCA TCTGATAGCA TGAGAACAAC AACTGTGGAT
    CCAGACGCAA GTACAGAAAC CCCTTACGAC TTTGTATTGG AAAATTTGAC ATGGAACGAG
    ACAGTGTATT ACTCTGAAAA TCCATTTTAT ATTACACCAA TTCCAAACAA AGAGCCCGGA
    GCTTTAACGA CTGCTATGAC CTGTCAATGT CGAAACGATT CTTCTCAGCC ATTTGTTTTA
    CTGAAGGAAA GCAATTGCTT GACAGAGTTT GGAAAAAATG GTGCATATAG TGCCTCCGTA
    TCTTTCAACC CAATGACCAG CTTTGTACCT GCAACTGGAA CTTATGAATT CTTAATTAAT
    GTTACCAATC GTGCATCTGG AGAATCTGCC AGTCACATCT TTACTATGAA TGTAGTTCTT
    CCAACTACAA CTACTGAAAC CCCACCAACC ACTGTCAGCT CGTCGGATGA TGCCGGTGGC
    AAAACCGGTG GAACTGGAGC TACTGGGGGA ACTGGTGGAA CAGGTTCAGG AGGATCTGCG
    ACAACTCTTA GCACGGGTGA TGCAGTCAGA TCAACCACTT CTGGATCTGG AAGTGGACAA
    TCATCAACGG GATCGGGGGC TGGTGGATCA GGAACGACCG CTTCTGGAAG TGGGTCAGGG
    GGGTCTTCAG GGACGGGTTC GGACGGGGTA AACTCTGGAA AAACAACGGC ATTGAATGGA
    GATGGTACAG GATCTGGAAC AGCTACCACA CCAGGATCCC ATTTAGGAGA CGGTGGTAGT
    ACATCTGGCT CAGGATCAGA TTCAAATGGA TCTTCTGGTG TTTCAACCAA GAGCTCATCT
    GGCTCCGATA CGTCTGGATC TTCCGATTCC TCTGGTGCAA ATGGTGCATT CTCTGCTACC
    GCGCAACCAT CTACAAGAAC TACTAAAACT CGCAGCTCTC TTGCAACAGT TTCTCCAATT
    TCTGCAGCTG AACAAGCGAT CATAGATGCT CAAAAAGCTG ATGTAATGAA CCAACTGGCT
    GGGATAATGG ATGGATCTGC ATCCAACAAC TCTCTAAACA CAAGCAGCTC TCTTCTCAAT
    CAAATCTCAT CTCTACCTGC CGCAGATCTA GTGGAAGTTG CTCAAAGTTT GCTCTCAAAT
    ACCCTCAAAA TCCCTGGAGT CGGAAATATG TCAAGTGTTG ATGTTTTGAA AACATTGCAA
    GACAATATTG CAACAACAAA TTCGGAATTG GCCGACGAAA TGGCAAAAGT TATCACTAAA
    CTAGCAAATG TCAATATGAC TTCCGCACAA TCCTTGAACT CCGTACTGTC TTCTCTCGAT
    CTTGCTCTCA AAGGAAGTAC AGTCTATACC TTGGGAGTAT CAAGTACAAA ATCAAAAGAT
    GGAACTTACG CGGTTATTTT TGGATATGTT ATTGCATCGG GATATACATT GGTATCGCCT
    AGGTGTACGC TCAGCATTTA CGGATCTACA ATATACCTTA CAGGAGATAC CCGGGCATCT
    TATAAACAGC TTGATGGAGA TACCGTTACT GCGGACACTA TGCTAGCAGC TGCCATCGGA
    ATCCAAGGAA TGTTCGCAAC AAATGGAAGG ACTGTGCAAG TTGAGCAGGA TAAAATAGAT
    GACAAACGAT CACTGGTTTC TGGAAATATA ATGGCTACAA TGAGTGGAGT TGGAGATGTT
    CAATCAGGGG AATATAGTTA TAATGATATG TATGTAACTG CTTGGAATGT CACTTATGAC
    AATTCAACAG TAGGATCCAC AAGTCAGAAG AACACTTCAT TTTCGTTCAA TATCCCAGTC
    TCAGAAGTTC AATACATATT ATTGATTGAA TCTGGAACAA TGATAAAACT GCATTCTACT
    CAAAATATTG TATCTAGAGG ACTAGTAGTA ACTGCATCAT ATGGTGGAGT TACTTACACA
    ATAACTTGCA CGAATGGAAC AGGAAAGTTT GTTGAAGTAG ACACAGATAA TGCCATTTTC
    TCATACAATG CTGACAGCTT CACGGTTGTG GCATCAGATG GATCCTCAGC GAGCACGGTT
    AAGAAGTTGA TTCAGATGCC GATAGTTATT GAGAACGTCA ACCTTGCTTT ATTCAATCAA
    ACTACTTCTC CACTTGTGTT CTCAAACGCG GGAAGTTATT CAATGCGAAT GGTGCTGTCT
    CCGCAGGACA TTGGAATTCC AGCTGTCTCT GCTTTATCTC AAACTGTCTC AATATCTACT
    CTTTCACCGA CGGCTTCGTA CACCAAAGAT GATTTGCAAT CATTGATTAA GGAACAAACA
    CTTGTCACCG TTTCGGGAAC TCTATTCTTC TCCAAAGCGT CATCCATCGA TGTCAGCGGC
    TACTCATTTT TCGTAGACTC CACCGCCCTT TATTTGTCAA ACTCTGTGAC AACTCTGGTG
    ATCAGTTCTC CCACCTACAA TATTGTTTCT TTGGCGCTTG GAGGTTACGG TATTCAGATA
    ACAGCCGGAA CTTACACATC TGGTTCTCAA ACTCATACGG TAACTTTAAT GGAATTCTCT
    GACACACAGA AAATGAGAAT CGATGGAGGG TTGATTATTA GAAATGGAAC GAATGGATAT
    GTAATTCAGA ATGGACAAGT TTCGACGGAA GGCGATGTTT CGGGAACTAA AATTGATATT
    GTGCCGCAAA GTTTGATGAA TCAAGAATCC CAGCAACAAA TTGAAACGAT TCTTAGCAAT
    ACTCAAGATT TTCTTACAAA TAATGGAATG ACTATGACAG ATGCAGAGAT TAATGATACA
    TCCAACTCTC TCCTTTCAAT TGCGGGATCT TTAACATCAG CGTTGAAGAT TGCTCTCGAC
    AATCCTCTTT CTTCTGATCT AGCTGCCAAT TTGAAATATG CAACAGATAA CTATGATTCC
    TTGTACAACG TGCTACCAAG TGATCCAGAC AATATTGTGT ACGTGGAGGA AATGACGTCA
    GAAGAATGGG CTGCTTACGT CACAAAAATG TTCCAGAAGA ACATCGCCAA AAATCTAGCC
    AACCAACTTG CATCTACACT AGATACCCTG GAAAACACGC TAGCAGCTAG AGCTATAGCA
    ACTGGCAACT TACCTTATGA CTACTCGAAT AGCGTCGACG GAACCGGAAT GGTAATCGTT
    ATCGATGATG CATCAAATAT TGTAGGAAAA ACTCAAAACT GTGAAGAATG GGCATTCAAA
    CTTCCATCGC CCGCTTCAAC TCTGAATACT GCTGAGATCA CAGATAAAAC ACTGATTCAA
    GTTGGATTAG TTTGCTATGC CACAAACCCG AGAACTTATG TGGATAACTT TGATATGTTA
    ATTACCTCAG GTGCACTTGA AGCACATATC AAAGATGAGA ATCAGATAAT CATTCCAATA
    ACCGGAACTA CTGCTCCAAT TTATGTGAAC GGCAGGGGAA GTGAGGATGA TGCAGTGCTA
    ACTTTAATGC AACAAGGAGA CTTTGCCAGT TATCAGATAT TAGATCTTCA TGCTTTTCGA
    ACCACTAACT GGAACAACTC GCTTCAAGTA GAGATTATTG CATCTCAAGA CTATGAAATT
    CCAAACAACG ATGACACCTA CATGTTCTCA TCATTCCAAA GTCTTCCTGG CCCACTTGAA
    TCAAATCATG AATGGATCTT TGACTTGAAC ACATTGAACA AGACAAGCAA TTATTTTGTC
    ACCGCCGGAA ATTTGATCAA CAACACTGGA CTTTTCTTTA TTGGAATTGG AAAAAGAAAT
    AGCTCTACTA ACACTGGAAA TAGTTCGGAT ATTGTAAACT ATGGTCAGTA CGATTCAATG
    CAGTGGTCAT TTGCACGAAG TGTACCAATG GATTATCAAG TCGCTGCAGT GAGTAAAGGA
    TGTTACTTCT ATCAGAAAAC ATCGGATGTC TTCAATTCTG AAGGAATGTA TCCAAGTGAT
    GGACAAGGAA TGCAGTTCGT TAATTGTTCA ACTGATCATT TAACAATGTT CTCTGTTGGT
    GCATTCAATC CAACTATTGA TGCAGATTTC TCTTACAATT ATAATGTGAA TGAAATCGAA
    AAGAATGTGA AAGTGATGAT TGCCGCTGTT TTCATGCTAG TAGTTTACGG ATGTCTTACC
    ATCAATGCAA TCATCTGTCA ACGTAAAGAT GCATCACGCG GGAGACTTCG TTTTCTGAAA
    GACAATGAGC CTCATGATGG ATACATGTAT GTGATTGCCG TGGAGACTGG ATATCGAATG
    TTTGCAACTA CGGATAGTAC GATATGTTTC AATTTATCTG GAAATGAAGG GGATCAGATT
    TTCCGATCGT TCAGATCAGA AGAAGATGGT AATTGGGAGT TTCCGTTTTC ATGGGGAACC
    ACTGATCGAT TTGTGATGAC AACGGCATTC CCTCTTGGCG AGTTGGAATA CATGAGACTC
    TGGCTTGACG ATGCTGGTCT AGATCACCGA GAGTCGTGGT ATTGCAATCG AATAATTGTG
    AAAGATCTAC AGACTCAGGA TATCTACTAC TTCCCTTTCA ACAATTGGCT TGGAACTAAA
    AACGGAGACG GAGAGACTGA AAGACTAGCG CGAGTGGAGT ATAAGCGAAG GTTTTTGGAT
    GAGTCAATGA GTATGCATAT GCTGGCTCAA ACAATTTCCT GGTTTGCCAT GTTTACTGGT
    GGAGGCAATC GGTTGAGGGA TCGAGTGTCA AGGCAAGACT ATTCTGTCAG TATCATCTTC
    TCACTTGTTG TGGTCAGCAT GATTAGCATC ACAATTCTGA AGAGCGATAA TTCGATTATC
    AGTGATTCGA AGAGTGTCTC CGAGTTCACT TTCACTATAA AAGATATTGC ATTCGGAGTT
    GGATTCGGTG TTCTTATCAC TTTCCTCAAC TCATTGCACA TTTTATTATG CACCAAATGT
    CGTTCCCACA GTGAACATTA CTACTACAAA AAACGAAAGA GAGAGGATCC GGAATTCAAG
    GATAATAGTG GTTCATGGCC GATGTTCATG GCTGGGATGG CACGTACCAT CATAGTATTT
    CCAGTTCTCA TGGGTCTTAT TTATATTTCT GGAGCTGGAA TGAGCTTGAT GGATGATTTA
    GCCAACTCTT TCTATATCCG ATTTCTGATC AGTCTGATTC TATGGGCAGT GGTGTTCGAG
    CCAATTAAGG GATTGATTTG GGCGTTTTTA ATTTTGAAAA CCAGAAAGAG TCATAAAATC
    ATTAACAAGT TGGAGGAAGC ATTGCTAAGA GCTAAGCCAG CTGAGACGTT CTTGAGAAAT
    CCCTATGGGA AAATCGAAAA AGGTCTGGGA ACCGAAATTG CTGATGTTAC AAAGCTCCGA
    GACACTGAAA ACCGAAAAAT GAGAGATGAA CAGTTATTTA TAACCATTCG TGATATGCTT
    TGTTTCTTTG CTTCTTTGTA TATTATGGTT ATGCTGACAT ACTATTGCAA AGACCGGCAT
    GGATATTGGT ATCAGTTGGA GATGAGCACA ATCTTGAACA TCAATCAAAA GAACTACGGT
    GACAATACTT TCATGTCAAT CCAACATGCT GACGATTTCT GGGATTGGGC TCGTGAATCT
    CTGGCCACTG CTCTTTTGGC TTCTTGGTAC GACGGAAATC CTGCATACGG GATGAGAGCC
    TACATGAATG ATAAAGTTTC AAGAAGTATG GGAATAGGTA CGATACGGCA GGTTCGGACA
    AAGAAAAGTG CAGAGTGTAC GATGTTCAAG CAGTTTCAAG GATACATCAA TGATTGTGGA
    GAGGAGTTGA CAAGCAAAAA CGAAGAAAAA ACTTTGTACA TGCAAGCTGG TTGGACCGAA
    CTGGAATCCG AGAATGGAAC AGATGCTTCA GATGAATACA CCTACAAGAC TTCTGAAGAA
    TTATCCACTG AAACAGTTTC AGGCCTACTG TATAGCTATT CAGGAGGTGG ATATACTATC
    AGTATGTCTG GTACCCAAGC GGAAATCATC ACTCTCTTCA ATAAACTTGA TTCGGAACGA
    TGGATCGATG ATCATACTCG AGCAGTCATC ATTGAGTTCT CTGCATACAA TGCTCAAATC
    AACTACTTTT CGGTGGTACA GTTGTTGGTG GAGATTCCAA AGTCAGGAAT TTATCTTCCT
    AATAGTTGGG TTGAATCAGT AAGACTTATC AAATCAGAGG GAAGCGATGG AACTGTGGTC
    AAGTATTATG AGATGCTTTA CATCTTTTTC TCAGTTCTCA TATTTGTAAA AGAGATTGTG
    TTCTACTTGT ATGGGCGGTA CAAGGTGATA ACAACAATGA AACCAACGAG AAATCCATTC
    AAAATTGTGT ATCAGCTGGC ACTGGGAAAC TTCTCACCAT GGAACTTCAT GGACTTGATA
    GTTGGAGCTC TCGCTGTTGC ATCGGTCCTA GCCTACACCA TCCGCCAACG AACCACAAAT
    AGAGCAATGG AAGATTTCAA TGCAAACAAT GGAAATTCGT ACATTAATTT GACTGAACAG
    AGAAATTGGG AGATTGTATT CAGTTATTGT CTTGCCGGAG CCGTATTCTT CACTTCTTGT
    AAAATGATTC GAATCTTGAG ATTCAACAGA AGAATTGGAG TATTAGCGGC TACACTGGAT
    AACGCTCTCG GAGCTATTGT GTCATTTGGA ATTGCTTTCC TGTTCTTCAG CATGACATTC
    AACTCTGTAT TGTACGCAGT TTTAGGAAAT AAAATGGGAG GATATCGGAG CTTAATGGCT
    ACCTTTCAAA CAGCATTGGC TGGAATGCTT GGAAAACTTG ATGTTACCTC TATTCAACCG
    ATTTCTCAAT TTGCGTTTGT TGTGATCATG CTTTATATGA TTGCCGGATC AAAGCTCGTC
    CTGCAACTTT ACGTCACTAT AATTATGTTC GAGTTCGAGG AAATTCGTAA TGACAGTGAG
    AAGCAGACCA ATGATTACGA AATAATCGAT CACATAAAAT ACAAGACAAA ACGTCGCTTG
    GGTCTTCTTG AACCAAAAGA TTTTGCTCCA GTTTCAATTG CTGACACTCA AAAAGACTTC
    CGTCTATTCC ACAGCGCTGT TGCCAAAGTC AATCTGCTTC ATCATCGTGC CACCAGAATG
    CTCCAAACTC AAGGTCAATA CCAAAACCAA ACAGTCATCA ACTACACACT CTCCTATGAT
    CCTGTCTCTG CTATTCACGA AACTGGACCA AAGAGATTCC AGAAGTGGCG GCTAAACGAT
    GTGGAGAAAG ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.