rha-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	rha-1
Entrez Gene ID	174417
Full Name	ATP-dependent RNA helicase A
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_063489.6	NP_495890.2	ATP-dependent RNA helicase A
NM_063489.5	NP_495890.2	ATP-dependent RNA helicase A
NM_001373779.1	NP_001360473.1	ATP-dependent RNA helicase A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	dhx9	NP_001188373.1
Homo sapiens (human)	DHX9	NP_001348.2
Pan troglodytes (chimpanzee)	DHX9	XP_003308679.1
Canis lupus familiaris (dog)	DHX9	XP_005622469.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006599	XP_316626.4
Bos taurus (cattle)	DHX9	NP_776461.1
Mus musculus (house mouse)	Dhx9	NP_031868.2
Drosophila melanogaster (fruit fly)	mle	NP_476641.1
Rattus norvegicus (Norway rat)	Dhx9	NP_001100654.1
Xenopus tropicalis (tropical clawed frog)	dhx9	XP_002937403.1
	DHX9	XP_001114405.1
Caenorhabditis elegans (roundworm)	rha-1	NP_495890.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RHA-1 is a conserved transcription regulation protein that controls germline proliferation and development in C. elegans
Title: RNA helicase A is important for germline transcriptional control, proliferation, and meiosis in C. elegans.

The following rha-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rha-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa337560	NM_063489.6 Latest version!	Caenorhabditis elegans ATP-dependent RNA helicase A (rha-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa850891	NM_001373779.1 Latest version!	Caenorhabditis elegans ATP-dependent RNA helicase A (rha-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa337560	NM_063489.5	Caenorhabditis elegans ATP-dependent RNA helicase A (rha-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rha-1 ( NM_063489.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495890.2 Caenorhabditis elegans ATP-dependent RNA helicase A (rha-1), partial mRNA.

Price & Availability↑

CloneID	OCa337560
Clone ID Related Accession (Same CDS sequence)	NM_063489.6 , NM_063489.5
Accession Version	NM_063489.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	ATP-dependent RNA helicase A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_063489.5. COMPLETENESS: incomplete on the 5' end.

ATGTCCAGAG ACGTCAAAGA ATTTCTATAT GCTTGGTTAG GAAAGAATAA GTATGGAAAT 
CCGACGTACG ATACAAAAAG TGAAACCAGG AGTGGTAGGC AGAGATTCAA ATGCGAACTT 
CGGATCACAG GATTTGGCTA CACTGCTTTC GGAAATTCCA CAAACAAAAA GGATGCTGCT 
ACCAATGCTG CTCAAGATTT CTGCCAATAC CTAGTCAGAG AAGGAAAAAT GCAACAGAGT 
GATATTCCGA CTCTAACGTC TTCGAGTCTT GAAGCTTCTT CCACGTGGCA AGATTCTGAA 
ACTGCTACAA TGTTTTGTGG TGGAGAAGAT GGCAACTCGT TCCAAGAGTC ACAGCAACCA 
ATTCCACAAA AAAGATTTCC TTGGAGTAAC AATGCATATC AACGTAACGA GGGCACACAT 
GAACAATATA TTACACAGAA AGCAGAAGAA ATTGCTGCAT CGGAAACTGT TGATCTAAAA 
TCAGAAATTC ATGGAGGATG GACGATGGAG AATTCAAAGA AAGCGCTGAA TGAGTTTCTT 
CAAAAAATGA GACTTCCACA GGTTAACTAT GGAACCAAAA TTCGAGAATC AAACACCGTT 
AAAACAATGG AGACGACTGC CCAAATCTTT GTTCCTCAAA TCAACAAAAA TCTCGTTGGA 
AAAGGTACTG GATCTAACAA GAAAGTTTCT GAAGCGGCGT GTGCAATGAA TATCGTTCGT 
CAGATGTTCC ATCTCAATAT TATGCAATCG TACAGTGGAC CAATCAAGAA GAGTAAAGTC 
AGCACGCTTC CTGAAATTGC GATTTCCATT CCAGAAGATC TCTCAACTCG AGTGACAGAA 
TATGTTCGGT CGTGCGGTCT TGAGTTGCCA GAAATCAACG AAACGAGTGC AACTCCGGAA 
GCTCCTACCT CACTGCTCAC GGATGTTAAA CTAGCGCAGT TCCCTGTTTC AGAAATTTGT 
TCCGCTTCGA ATATCTCCTG GGCACCTCCT CTTCAGAATT GGAATCCATG GAGAGCTTCG 
AATATCGATG AGGAACCATT AGCTTTTATG TCAATGGAGC AAATTAGTCA GAGAATAATG 
GAAAAAGAGG ATTTCAAGCG AGGTGAAGCA CTTGACAAAA TAACAGCTCA ACGTGGAGAA 
CTCCCTGTCG CCCAGTATCG TGAAAACATT GTACAAACCG TCGCAGAAAA TCGTGTTACA 
CTGATCAAAG GAGAAACTGG ATGTGGAAAG AGTACTCAAG TTGCTCAATT TCTACTGGAA 
AGTTTTTTGG AAAATTCTAA TGGAGCATCT TTCAATGCAG TCGTCTCTCA ACCTCGTCGT 
ATTTCTGCAA TTTCACTGGC CGAACGTGTC GCCAACGAGC GAGGAGAAGA AGTTGGAGAA 
ACATGCGGAT ATAATGTTCG ATTCGATAGT GCAACTCCTC GGCCATATGG ATCTATCATG 
TTTTGTACTG TTGGAGTCTT GCTGCGAATG ATGGAGAACG GATTACGAGG AATTTCACAT 
GTAATCATCG ATGAAATTCA CGAACGGGAT GTTGACACTG ACTTTGTTCT TATTGTGCTC 
CGCGAAATGA TTAGCACATA CCGAGATCTA CGTGTGGTAC TCATGTCTGC CACCATCGAC 
ACTGATTTGT TTACCAATTT CTTCAGCTCT ATCCCAGATG TTGGCCCGAC ACCAGTTATC 
ACGATGCATG GAAGAACTTT TCCTGTACAA TCATTCTACC TGGAAGACAT TCTTCACAAT 
CTTCAGCATA TGCCTGAGGA ACCAGATCAA AAGAAACGGA AAAAGGGAGG ACCACCTCCA 
CCAGATGATG ATGAAGGAGA TGAAGAAGTG GATGATAAGG GTCGAAACAT GAATATTCTA 
ACAGATCCAT CAATCAACGA ATCACTGAAA ACAGCAATGT CAAGAATCTC TGAAAAAGAT 
ATTCCATTCG GTGTGATCGA AGCTATTTTG AACGATATTG CATCCCGCGG AGTGGATGGC 
GCCGTTTTAG TTTTTCTTCC CGGTTGGGCA GAAATTATGA CACTTTGCAA TCGACTTTTA 
GAGCACCAAG AATTTGGCCA AGCTAATAAA TATGAAATTC TTCCACTTCA TTCTCAATTA 
ACTAGTCAGG AACAGAGAAA AGTGTTCAAT CACTACCCAG GAAAGCGGAA GATCATTGTT 
TCAACAAATA TCGCTGAAAC AAGTATTACA ATTGATGACG TAGTCTATGT GATTGACTCT 
TGCAAAGCAA AGGAACGAAT GTATACATCG AACAACAATA TGGTTCATTT CGCAACTGTC 
TGGGCATCAA AGACAAATGT AATTCAAAGA AGAGGGCGCG CTGGTCGTGT TAGAGCTGGA 
TACGCTTTTC ATTTATGCAG TAAGATGAGA TTTGAAGCAC TGGATGATCA CGGAACTGCT 
GAAATGCTTC GGATTCCTCT TCATCAAATC GCTTTGACAA TCAAACTTCT CCGTCTCGGA 
TCCGTCGGTG AATTCCTTGG AAAGGCTCTT CAACCTCCAC CGTATGATAT GGTCGTCGAA 
AGTGAAGCAG TGTTACAAGC GATGGGAGCT CTTGATCGTA ATTTAGAATT GACAAGTCTC 
GGAAAAATGC TCGCTCGTAT GCCAATTGAG CCTGTCATTG CCAAAGTGCT TATTCTTGGA 
ACTGCTCTTG GTGCAGGATC TGTGATGTGT GACGTGGCAT CTGCAATGTC ATTTCCAACT 
CCATTCGTTC CACGAGAGAA GCATCACAGC CGATTGAGTG GTACACAGCG AAAGTTTGCT 
GGAAACAAGT TTTCTGATCA TGTTGCAATT GTCTCTGTGA TTCAAGGATA TCGTGAAGCC 
GTTCAAATGG GTGCATCTGC TGCTGAGCGC GAATTCTGTG AGCGATACTC GCTGAGTAAT 
CCTGTGCTCA AAATGACTGA TGGAGCACGT AGACAGTTGA TTGATGTACT TCGAAATCAA 
TGCAGTTTCC CTGAAGACAT TTTGTTTGAT ATCTCTGTTA ACGTCAATGG ACCAGATCGT 
GAACTTAATC TGATGCGTTC TCTTCTTGTC ATGGCTCTTT ATCCAAATGT GGCGTACTAC 
GTTGGAAAAC GGAAAGTATT AACGATAGAG CAATCGAGTG CACTAATCAA CAAGTATTCT 
ATGCTTGTTC CGATGAATAA TCGCCAAGAA ATGGATTTCC CAAGTCCTCT GCTCGTTTTC 
ACTGAAAAAG TTCGTACCAG ATGCATCTCA TGTAAACAAA TGTCAGTGAT CAGTGCAATA 
CAATTGTTAG TTTTTGGAAG TCGAAAAGTT GAATGTGTCG GAGAAGGACT TGTTCGCATT 
GATGAGACTA TTACAATTCG AATGAACGTT TCTACCGCAG CTGCTCTCAT CGGTCTTCGT 
CCATGCATCG AAGCTCTTCT CGTCAAATCT TGTGAAAATC CAGAATCTCT GGCTGGCCTC 
AACCCATCAG ATGCTGAACT TCGTCAACTT CTTCGTGACA TATCTTCCGA AGAATTCATG 
TCCGAAGCTG GACCTATCAA AGACAGTCTA CTTACAGATA ATGCGTTAAT CCAGAAAGGC 
CCTGCTCCAA ATCGTCTTGA ATATGCTGAT TGGGGACCGT CGAACAACAA TTCATCATTC 
CAAAATCAAG ATTTTCCACC AGCTGCTGGG GGCAAAGTGC ACCCTTACCG CGGTAACAGA 
CGCGGAAATC ATCCATATGC TCAAAATCGT CCCTATGCTC CTCCCAATTC CGGAATGGGC 
TATCAGAATT TCAACAACTC AGGTTACGGC GCCAGTGGCG ATTGGAACGC CGGACGTGGC 
AATTACGGCA ATGTTGGAAG CGGATATCGA GGAGCTGGCG GCGGCGGCGG TTACCGAGGT 
GGTCGTGGTG GAAGAGGTGG TGGCCGTGGC GGTGGTGGCA GAGGATGGAA TGCATCGAAC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495890.2
CDS	1..3906
Translation	MSRDVKEFLYAWLGKNKYGNPTYDTKSETRSGRQRFKCELRITGFGYTAFGNSTNKKDAATNAAQDFCQYLVREGKMQQSDIPTLTSSSLEASSTWQDSETATMFCGGEDGNSFQESQQPIPQKRFPWSNNAYQRNEGTHEQYITQKAEEIAASETVDLKSEIHGGWTMENSKKALNEFLQKMRLPQVNYGTKIRESNTVKTMETTAQIFVPQINKNLVGKGTGSNKKVSEAACAMNIVRQMFHLNIMQSYSGPIKKSKVSTLPEIAISIPEDLSTRVTEYVRSCGLELPEINETSATPEAPTSLLTDVKLAQFPVSEICSASNISWAPPLQNWNPWRASNIDEEPLAFMSMEQISQRIMEKEDFKRGEALDKITAQRGELPVAQYRENIVQTVAENRVTLIKGETGCGKSTQVAQFLLESFLENSNGASFNAVVSQPRRISAISLAERVANERGEEVGETCGYNVRFDSATPRPYGSIMFCTVGVLLRMMENGLRGISHVIIDEIHERDVDTDFVLIVLREMISTYRDLRVVLMSATIDTDLFTNFFSSIPDVGPTPVITMHGRTFPVQSFYLEDILHNLQHMPEEPDQKKRKKGGPPPPDDDEGDEEVDDKGRNMNILTDPSINESLKTAMSRISEKDIPFGVIEAILNDIASRGVDGAVLVFLPGWAEIMTLCNRLLEHQEFGQANKYEILPLHSQLTSQEQRKVFNHYPGKRKIIVSTNIAETSITIDDVVYVIDSCKAKERMYTSNNNMVHFATVWASKTNVIQRRGRAGRVRAGYAFHLCSKMRFEALDDHGTAEMLRIPLHQIALTIKLLRLGSVGEFLGKALQPPPYDMVVESEAVLQAMGALDRNLELTSLGKMLARMPIEPVIAKVLILGTALGAGSVMCDVASAMSFPTPFVPREKHHSRLSGTQRKFAGNKFSDHVAIVSVIQGYREAVQMGASAAEREFCERYSLSNPVLKMTDGARRQLIDVLRNQCSFPEDILFDISVNVNGPDRELNLMRSLLVMALYPNVAYYVGKRKVLTIEQSSALINKYSMLVPMNNRQEMDFPSPLLVFTEKVRTRCISCKQMSVISAIQLLVFGSRKVECVGEGLVRIDETITIRMNVSTAAALIGLRPCIEALLVKSCENPESLAGLNPSDAELRQLLRDISSEEFMSEAGPIKDSLLTDNALIQKGPAPNRLEYADWGPSNNNSSFQNQDFPPAAGGKVHPYRGNRRGNHPYAQNRPYAPPNSGMGYQNFNNSGYGASGDWNAGRGNYGNVGSGYRGAGGGGGYRGGRGGRGGGRGGGGRGWNASNW

Target ORF information:

RefSeq Version	NM_063489.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans ATP-dependent RNA helicase A (rha-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063489.6

ORF Insert Sequence:

ATGTCCAGAG ACGTCAAAGA ATTTCTATAT GCTTGGTTAG GAAAGAATAA GTATGGAAAT 
CCGACGTACG ATACAAAAAG TGAAACCAGG AGTGGTAGGC AGAGATTCAA ATGCGAACTT 
CGGATCACAG GATTTGGCTA CACTGCTTTC GGAAATTCCA CAAACAAAAA GGATGCTGCT 
ACCAATGCTG CTCAAGATTT CTGCCAATAC CTAGTCAGAG AAGGAAAAAT GCAACAGAGT 
GATATTCCGA CTCTAACGTC TTCGAGTCTT GAAGCTTCTT CCACGTGGCA AGATTCTGAA 
ACTGCTACAA TGTTTTGTGG TGGAGAAGAT GGCAACTCGT TCCAAGAGTC ACAGCAACCA 
ATTCCACAAA AAAGATTTCC TTGGAGTAAC AATGCATATC AACGTAACGA GGGCACACAT 
GAACAATATA TTACACAGAA AGCAGAAGAA ATTGCTGCAT CGGAAACTGT TGATCTAAAA 
TCAGAAATTC ATGGAGGATG GACGATGGAG AATTCAAAGA AAGCGCTGAA TGAGTTTCTT 
CAAAAAATGA GACTTCCACA GGTTAACTAT GGAACCAAAA TTCGAGAATC AAACACCGTT 
AAAACAATGG AGACGACTGC CCAAATCTTT GTTCCTCAAA TCAACAAAAA TCTCGTTGGA 
AAAGGTACTG GATCTAACAA GAAAGTTTCT GAAGCGGCGT GTGCAATGAA TATCGTTCGT 
CAGATGTTCC ATCTCAATAT TATGCAATCG TACAGTGGAC CAATCAAGAA GAGTAAAGTC 
AGCACGCTTC CTGAAATTGC GATTTCCATT CCAGAAGATC TCTCAACTCG AGTGACAGAA 
TATGTTCGGT CGTGCGGTCT TGAGTTGCCA GAAATCAACG AAACGAGTGC AACTCCGGAA 
GCTCCTACCT CACTGCTCAC GGATGTTAAA CTAGCGCAGT TCCCTGTTTC AGAAATTTGT 
TCCGCTTCGA ATATCTCCTG GGCACCTCCT CTTCAGAATT GGAATCCATG GAGAGCTTCG 
AATATCGATG AGGAACCATT AGCTTTTATG TCAATGGAGC AAATTAGTCA GAGAATAATG 
GAAAAAGAGG ATTTCAAGCG AGGTGAAGCA CTTGACAAAA TAACAGCTCA ACGTGGAGAA 
CTCCCTGTCG CCCAGTATCG TGAAAACATT GTACAAACCG TCGCAGAAAA TCGTGTTACA 
CTGATCAAAG GAGAAACTGG ATGTGGAAAG AGTACTCAAG TTGCTCAATT TCTACTGGAA 
AGTTTTTTGG AAAATTCTAA TGGAGCATCT TTCAATGCAG TCGTCTCTCA ACCTCGTCGT 
ATTTCTGCAA TTTCACTGGC CGAACGTGTC GCCAACGAGC GAGGAGAAGA AGTTGGAGAA 
ACATGCGGAT ATAATGTTCG ATTCGATAGT GCAACTCCTC GGCCATATGG ATCTATCATG 
TTTTGTACTG TTGGAGTCTT GCTGCGAATG ATGGAGAACG GATTACGAGG AATTTCACAT 
GTAATCATCG ATGAAATTCA CGAACGGGAT GTTGACACTG ACTTTGTTCT TATTGTGCTC 
CGCGAAATGA TTAGCACATA CCGAGATCTA CGTGTGGTAC TCATGTCTGC CACCATCGAC 
ACTGATTTGT TTACCAATTT CTTCAGCTCT ATCCCAGATG TTGGCCCGAC ACCAGTTATC 
ACGATGCATG GAAGAACTTT TCCTGTACAA TCATTCTACC TGGAAGACAT TCTTCACAAT 
CTTCAGCATA TGCCTGAGGA ACCAGATCAA AAGAAACGGA AAAAGGGAGG ACCACCTCCA 
CCAGATGATG ATGAAGGAGA TGAAGAAGTG GATGATAAGG GTCGAAACAT GAATATTCTA 
ACAGATCCAT CAATCAACGA ATCACTGAAA ACAGCAATGT CAAGAATCTC TGAAAAAGAT 
ATTCCATTCG GTGTGATCGA AGCTATTTTG AACGATATTG CATCCCGCGG AGTGGATGGC 
GCCGTTTTAG TTTTTCTTCC CGGTTGGGCA GAAATTATGA CACTTTGCAA TCGACTTTTA 
GAGCACCAAG AATTTGGCCA AGCTAATAAA TATGAAATTC TTCCACTTCA TTCTCAATTA 
ACTAGTCAGG AACAGAGAAA AGTGTTCAAT CACTACCCAG GAAAGCGGAA GATCATTGTT 
TCAACAAATA TCGCTGAAAC AAGTATTACA ATTGATGACG TAGTCTATGT GATTGACTCT 
TGCAAAGCAA AGGAACGAAT GTATACATCG AACAACAATA TGGTTCATTT CGCAACTGTC 
TGGGCATCAA AGACAAATGT AATTCAAAGA AGAGGGCGCG CTGGTCGTGT TAGAGCTGGA 
TACGCTTTTC ATTTATGCAG TAAGATGAGA TTTGAAGCAC TGGATGATCA CGGAACTGCT 
GAAATGCTTC GGATTCCTCT TCATCAAATC GCTTTGACAA TCAAACTTCT CCGTCTCGGA 
TCCGTCGGTG AATTCCTTGG AAAGGCTCTT CAACCTCCAC CGTATGATAT GGTCGTCGAA 
AGTGAAGCAG TGTTACAAGC GATGGGAGCT CTTGATCGTA ATTTAGAATT GACAAGTCTC 
GGAAAAATGC TCGCTCGTAT GCCAATTGAG CCTGTCATTG CCAAAGTGCT TATTCTTGGA 
ACTGCTCTTG GTGCAGGATC TGTGATGTGT GACGTGGCAT CTGCAATGTC ATTTCCAACT 
CCATTCGTTC CACGAGAGAA GCATCACAGC CGATTGAGTG GTACACAGCG AAAGTTTGCT 
GGAAACAAGT TTTCTGATCA TGTTGCAATT GTCTCTGTGA TTCAAGGATA TCGTGAAGCC 
GTTCAAATGG GTGCATCTGC TGCTGAGCGC GAATTCTGTG AGCGATACTC GCTGAGTAAT 
CCTGTGCTCA AAATGACTGA TGGAGCACGT AGACAGTTGA TTGATGTACT TCGAAATCAA 
TGCAGTTTCC CTGAAGACAT TTTGTTTGAT ATCTCTGTTA ACGTCAATGG ACCAGATCGT 
GAACTTAATC TGATGCGTTC TCTTCTTGTC ATGGCTCTTT ATCCAAATGT GGCGTACTAC 
GTTGGAAAAC GGAAAGTATT AACGATAGAG CAATCGAGTG CACTAATCAA CAAGTATTCT 
ATGCTTGTTC CGATGAATAA TCGCCAAGAA ATGGATTTCC CAAGTCCTCT GCTCGTTTTC 
ACTGAAAAAG TTCGTACCAG ATGCATCTCA TGTAAACAAA TGTCAGTGAT CAGTGCAATA 
CAATTGTTAG TTTTTGGAAG TCGAAAAGTT GAATGTGTCG GAGAAGGACT TGTTCGCATT 
GATGAGACTA TTACAATTCG AATGAACGTT TCTACCGCAG CTGCTCTCAT CGGTCTTCGT 
CCATGCATCG AAGCTCTTCT CGTCAAATCT TGTGAAAATC CAGAATCTCT GGCTGGCCTC 
AACCCATCAG ATGCTGAACT TCGTCAACTT CTTCGTGACA TATCTTCCGA AGAATTCATG 
TCCGAAGCTG GACCTATCAA AGACAGTCTA CTTACAGATA ATGCGTTAAT CCAGAAAGGC 
CCTGCTCCAA ATCGTCTTGA ATATGCTGAT TGGGGACCGT CGAACAACAA TTCATCATTC 
CAAAATCAAG ATTTTCCACC AGCTGCTGGG GGCAAAGTGC ACCCTTACCG CGGTAACAGA 
CGCGGAAATC ATCCATATGC TCAAAATCGT CCCTATGCTC CTCCCAATTC CGGAATGGGC 
TATCAGAATT TCAACAACTC AGGTTACGGC GCCAGTGGCG ATTGGAACGC CGGACGTGGC 
AATTACGGCA ATGTTGGAAG CGGATATCGA GGAGCTGGCG GCGGCGGCGG TTACCGAGGT 
GGTCGTGGTG GAAGAGGTGG TGGCCGTGGC GGTGGTGGCA GAGGATGGAA TGCATCGAAC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rha-1 ( NM_063489.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495890.2 Caenorhabditis elegans ATP-dependent RNA helicase A (rha-1), partial mRNA.

Price & Availability↑

CloneID	OCa337560
Clone ID Related Accession (Same CDS sequence)	NM_063489.6 , NM_063489.5
Accession Version	NM_063489.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	ATP-dependent RNA helicase A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_063489.4. COMPLETENESS: incomplete on both ends.

ATGTCCAGAG ACGTCAAAGA ATTTCTATAT GCTTGGTTAG GAAAGAATAA GTATGGAAAT 
CCGACGTACG ATACAAAAAG TGAAACCAGG AGTGGTAGGC AGAGATTCAA ATGCGAACTT 
CGGATCACAG GATTTGGCTA CACTGCTTTC GGAAATTCCA CAAACAAAAA GGATGCTGCT 
ACCAATGCTG CTCAAGATTT CTGCCAATAC CTAGTCAGAG AAGGAAAAAT GCAACAGAGT 
GATATTCCGA CTCTAACGTC TTCGAGTCTT GAAGCTTCTT CCACGTGGCA AGATTCTGAA 
ACTGCTACAA TGTTTTGTGG TGGAGAAGAT GGCAACTCGT TCCAAGAGTC ACAGCAACCA 
ATTCCACAAA AAAGATTTCC TTGGAGTAAC AATGCATATC AACGTAACGA GGGCACACAT 
GAACAATATA TTACACAGAA AGCAGAAGAA ATTGCTGCAT CGGAAACTGT TGATCTAAAA 
TCAGAAATTC ATGGAGGATG GACGATGGAG AATTCAAAGA AAGCGCTGAA TGAGTTTCTT 
CAAAAAATGA GACTTCCACA GGTTAACTAT GGAACCAAAA TTCGAGAATC AAACACCGTT 
AAAACAATGG AGACGACTGC CCAAATCTTT GTTCCTCAAA TCAACAAAAA TCTCGTTGGA 
AAAGGTACTG GATCTAACAA GAAAGTTTCT GAAGCGGCGT GTGCAATGAA TATCGTTCGT 
CAGATGTTCC ATCTCAATAT TATGCAATCG TACAGTGGAC CAATCAAGAA GAGTAAAGTC 
AGCACGCTTC CTGAAATTGC GATTTCCATT CCAGAAGATC TCTCAACTCG AGTGACAGAA 
TATGTTCGGT CGTGCGGTCT TGAGTTGCCA GAAATCAACG AAACGAGTGC AACTCCGGAA 
GCTCCTACCT CACTGCTCAC GGATGTTAAA CTAGCGCAGT TCCCTGTTTC AGAAATTTGT 
TCCGCTTCGA ATATCTCCTG GGCACCTCCT CTTCAGAATT GGAATCCATG GAGAGCTTCG 
AATATCGATG AGGAACCATT AGCTTTTATG TCAATGGAGC AAATTAGTCA GAGAATAATG 
GAAAAAGAGG ATTTCAAGCG AGGTGAAGCA CTTGACAAAA TAACAGCTCA ACGTGGAGAA 
CTCCCTGTCG CCCAGTATCG TGAAAACATT GTACAAACCG TCGCAGAAAA TCGTGTTACA 
CTGATCAAAG GAGAAACTGG ATGTGGAAAG AGTACTCAAG TTGCTCAATT TCTACTGGAA 
AGTTTTTTGG AAAATTCTAA TGGAGCATCT TTCAATGCAG TCGTCTCTCA ACCTCGTCGT 
ATTTCTGCAA TTTCACTGGC CGAACGTGTC GCCAACGAGC GAGGAGAAGA AGTTGGAGAA 
ACATGCGGAT ATAATGTTCG ATTCGATAGT GCAACTCCTC GGCCATATGG ATCTATCATG 
TTTTGTACTG TTGGAGTCTT GCTGCGAATG ATGGAGAACG GATTACGAGG AATTTCACAT 
GTAATCATCG ATGAAATTCA CGAACGGGAT GTTGACACTG ACTTTGTTCT TATTGTGCTC 
CGCGAAATGA TTAGCACATA CCGAGATCTA CGTGTGGTAC TCATGTCTGC CACCATCGAC 
ACTGATTTGT TTACCAATTT CTTCAGCTCT ATCCCAGATG TTGGCCCGAC ACCAGTTATC 
ACGATGCATG GAAGAACTTT TCCTGTACAA TCATTCTACC TGGAAGACAT TCTTCACAAT 
CTTCAGCATA TGCCTGAGGA ACCAGATCAA AAGAAACGGA AAAAGGGAGG ACCACCTCCA 
CCAGATGATG ATGAAGGAGA TGAAGAAGTG GATGATAAGG GTCGAAACAT GAATATTCTA 
ACAGATCCAT CAATCAACGA ATCACTGAAA ACAGCAATGT CAAGAATCTC TGAAAAAGAT 
ATTCCATTCG GTGTGATCGA AGCTATTTTG AACGATATTG CATCCCGCGG AGTGGATGGC 
GCCGTTTTAG TTTTTCTTCC CGGTTGGGCA GAAATTATGA CACTTTGCAA TCGACTTTTA 
GAGCACCAAG AATTTGGCCA AGCTAATAAA TATGAAATTC TTCCACTTCA TTCTCAATTA 
ACTAGTCAGG AACAGAGAAA AGTGTTCAAT CACTACCCAG GAAAGCGGAA GATCATTGTT 
TCAACAAATA TCGCTGAAAC AAGTATTACA ATTGATGACG TAGTCTATGT GATTGACTCT 
TGCAAAGCAA AGGAACGAAT GTATACATCG AACAACAATA TGGTTCATTT CGCAACTGTC 
TGGGCATCAA AGACAAATGT AATTCAAAGA AGAGGGCGCG CTGGTCGTGT TAGAGCTGGA 
TACGCTTTTC ATTTATGCAG TAAGATGAGA TTTGAAGCAC TGGATGATCA CGGAACTGCT 
GAAATGCTTC GGATTCCTCT TCATCAAATC GCTTTGACAA TCAAACTTCT CCGTCTCGGA 
TCCGTCGGTG AATTCCTTGG AAAGGCTCTT CAACCTCCAC CGTATGATAT GGTCGTCGAA 
AGTGAAGCAG TGTTACAAGC GATGGGAGCT CTTGATCGTA ATTTAGAATT GACAAGTCTC 
GGAAAAATGC TCGCTCGTAT GCCAATTGAG CCTGTCATTG CCAAAGTGCT TATTCTTGGA 
ACTGCTCTTG GTGCAGGATC TGTGATGTGT GACGTGGCAT CTGCAATGTC ATTTCCAACT 
CCATTCGTTC CACGAGAGAA GCATCACAGC CGATTGAGTG GTACACAGCG AAAGTTTGCT 
GGAAACAAGT TTTCTGATCA TGTTGCAATT GTCTCTGTGA TTCAAGGATA TCGTGAAGCC 
GTTCAAATGG GTGCATCTGC TGCTGAGCGC GAATTCTGTG AGCGATACTC GCTGAGTAAT 
CCTGTGCTCA AAATGACTGA TGGAGCACGT AGACAGTTGA TTGATGTACT TCGAAATCAA 
TGCAGTTTCC CTGAAGACAT TTTGTTTGAT ATCTCTGTTA ACGTCAATGG ACCAGATCGT 
GAACTTAATC TGATGCGTTC TCTTCTTGTC ATGGCTCTTT ATCCAAATGT GGCGTACTAC 
GTTGGAAAAC GGAAAGTATT AACGATAGAG CAATCGAGTG CACTAATCAA CAAGTATTCT 
ATGCTTGTTC CGATGAATAA TCGCCAAGAA ATGGATTTCC CAAGTCCTCT GCTCGTTTTC 
ACTGAAAAAG TTCGTACCAG ATGCATCTCA TGTAAACAAA TGTCAGTGAT CAGTGCAATA 
CAATTGTTAG TTTTTGGAAG TCGAAAAGTT GAATGTGTCG GAGAAGGACT TGTTCGCATT 
GATGAGACTA TTACAATTCG AATGAACGTT TCTACCGCAG CTGCTCTCAT CGGTCTTCGT 
CCATGCATCG AAGCTCTTCT CGTCAAATCT TGTGAAAATC CAGAATCTCT GGCTGGCCTC 
AACCCATCAG ATGCTGAACT TCGTCAACTT CTTCGTGACA TATCTTCCGA AGAATTCATG 
TCCGAAGCTG GACCTATCAA AGACAGTCTA CTTACAGATA ATGCGTTAAT CCAGAAAGGC 
CCTGCTCCAA ATCGTCTTGA ATATGCTGAT TGGGGACCGT CGAACAACAA TTCATCATTC 
CAAAATCAAG ATTTTCCACC AGCTGCTGGG GGCAAAGTGC ACCCTTACCG CGGTAACAGA 
CGCGGAAATC ATCCATATGC TCAAAATCGT CCCTATGCTC CTCCCAATTC CGGAATGGGC 
TATCAGAATT TCAACAACTC AGGTTACGGC GCCAGTGGCG ATTGGAACGC CGGACGTGGC 
AATTACGGCA ATGTTGGAAG CGGATATCGA GGAGCTGGCG GCGGCGGCGG TTACCGAGGT 
GGTCGTGGTG GAAGAGGTGG TGGCCGTGGC GGTGGTGGCA GAGGATGGAA TGCATCGAAC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495890.2
CDS	1..3906
Translation	MSRDVKEFLYAWLGKNKYGNPTYDTKSETRSGRQRFKCELRITGFGYTAFGNSTNKKDAATNAAQDFCQYLVREGKMQQSDIPTLTSSSLEASSTWQDSETATMFCGGEDGNSFQESQQPIPQKRFPWSNNAYQRNEGTHEQYITQKAEEIAASETVDLKSEIHGGWTMENSKKALNEFLQKMRLPQVNYGTKIRESNTVKTMETTAQIFVPQINKNLVGKGTGSNKKVSEAACAMNIVRQMFHLNIMQSYSGPIKKSKVSTLPEIAISIPEDLSTRVTEYVRSCGLELPEINETSATPEAPTSLLTDVKLAQFPVSEICSASNISWAPPLQNWNPWRASNIDEEPLAFMSMEQISQRIMEKEDFKRGEALDKITAQRGELPVAQYRENIVQTVAENRVTLIKGETGCGKSTQVAQFLLESFLENSNGASFNAVVSQPRRISAISLAERVANERGEEVGETCGYNVRFDSATPRPYGSIMFCTVGVLLRMMENGLRGISHVIIDEIHERDVDTDFVLIVLREMISTYRDLRVVLMSATIDTDLFTNFFSSIPDVGPTPVITMHGRTFPVQSFYLEDILHNLQHMPEEPDQKKRKKGGPPPPDDDEGDEEVDDKGRNMNILTDPSINESLKTAMSRISEKDIPFGVIEAILNDIASRGVDGAVLVFLPGWAEIMTLCNRLLEHQEFGQANKYEILPLHSQLTSQEQRKVFNHYPGKRKIIVSTNIAETSITIDDVVYVIDSCKAKERMYTSNNNMVHFATVWASKTNVIQRRGRAGRVRAGYAFHLCSKMRFEALDDHGTAEMLRIPLHQIALTIKLLRLGSVGEFLGKALQPPPYDMVVESEAVLQAMGALDRNLELTSLGKMLARMPIEPVIAKVLILGTALGAGSVMCDVASAMSFPTPFVPREKHHSRLSGTQRKFAGNKFSDHVAIVSVIQGYREAVQMGASAAEREFCERYSLSNPVLKMTDGARRQLIDVLRNQCSFPEDILFDISVNVNGPDRELNLMRSLLVMALYPNVAYYVGKRKVLTIEQSSALINKYSMLVPMNNRQEMDFPSPLLVFTEKVRTRCISCKQMSVISAIQLLVFGSRKVECVGEGLVRIDETITIRMNVSTAAALIGLRPCIEALLVKSCENPESLAGLNPSDAELRQLLRDISSEEFMSEAGPIKDSLLTDNALIQKGPAPNRLEYADWGPSNNNSSFQNQDFPPAAGGKVHPYRGNRRGNHPYAQNRPYAPPNSGMGYQNFNNSGYGASGDWNAGRGNYGNVGSGYRGAGGGGGYRGGRGGRGGGRGGGGRGWNASNW

Target ORF information:

RefSeq Version	NM_063489.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans ATP-dependent RNA helicase A (rha-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063489.5

ORF Insert Sequence:

ATGTCCAGAG ACGTCAAAGA ATTTCTATAT GCTTGGTTAG GAAAGAATAA GTATGGAAAT 
CCGACGTACG ATACAAAAAG TGAAACCAGG AGTGGTAGGC AGAGATTCAA ATGCGAACTT 
CGGATCACAG GATTTGGCTA CACTGCTTTC GGAAATTCCA CAAACAAAAA GGATGCTGCT 
ACCAATGCTG CTCAAGATTT CTGCCAATAC CTAGTCAGAG AAGGAAAAAT GCAACAGAGT 
GATATTCCGA CTCTAACGTC TTCGAGTCTT GAAGCTTCTT CCACGTGGCA AGATTCTGAA 
ACTGCTACAA TGTTTTGTGG TGGAGAAGAT GGCAACTCGT TCCAAGAGTC ACAGCAACCA 
ATTCCACAAA AAAGATTTCC TTGGAGTAAC AATGCATATC AACGTAACGA GGGCACACAT 
GAACAATATA TTACACAGAA AGCAGAAGAA ATTGCTGCAT CGGAAACTGT TGATCTAAAA 
TCAGAAATTC ATGGAGGATG GACGATGGAG AATTCAAAGA AAGCGCTGAA TGAGTTTCTT 
CAAAAAATGA GACTTCCACA GGTTAACTAT GGAACCAAAA TTCGAGAATC AAACACCGTT 
AAAACAATGG AGACGACTGC CCAAATCTTT GTTCCTCAAA TCAACAAAAA TCTCGTTGGA 
AAAGGTACTG GATCTAACAA GAAAGTTTCT GAAGCGGCGT GTGCAATGAA TATCGTTCGT 
CAGATGTTCC ATCTCAATAT TATGCAATCG TACAGTGGAC CAATCAAGAA GAGTAAAGTC 
AGCACGCTTC CTGAAATTGC GATTTCCATT CCAGAAGATC TCTCAACTCG AGTGACAGAA 
TATGTTCGGT CGTGCGGTCT TGAGTTGCCA GAAATCAACG AAACGAGTGC AACTCCGGAA 
GCTCCTACCT CACTGCTCAC GGATGTTAAA CTAGCGCAGT TCCCTGTTTC AGAAATTTGT 
TCCGCTTCGA ATATCTCCTG GGCACCTCCT CTTCAGAATT GGAATCCATG GAGAGCTTCG 
AATATCGATG AGGAACCATT AGCTTTTATG TCAATGGAGC AAATTAGTCA GAGAATAATG 
GAAAAAGAGG ATTTCAAGCG AGGTGAAGCA CTTGACAAAA TAACAGCTCA ACGTGGAGAA 
CTCCCTGTCG CCCAGTATCG TGAAAACATT GTACAAACCG TCGCAGAAAA TCGTGTTACA 
CTGATCAAAG GAGAAACTGG ATGTGGAAAG AGTACTCAAG TTGCTCAATT TCTACTGGAA 
AGTTTTTTGG AAAATTCTAA TGGAGCATCT TTCAATGCAG TCGTCTCTCA ACCTCGTCGT 
ATTTCTGCAA TTTCACTGGC CGAACGTGTC GCCAACGAGC GAGGAGAAGA AGTTGGAGAA 
ACATGCGGAT ATAATGTTCG ATTCGATAGT GCAACTCCTC GGCCATATGG ATCTATCATG 
TTTTGTACTG TTGGAGTCTT GCTGCGAATG ATGGAGAACG GATTACGAGG AATTTCACAT 
GTAATCATCG ATGAAATTCA CGAACGGGAT GTTGACACTG ACTTTGTTCT TATTGTGCTC 
CGCGAAATGA TTAGCACATA CCGAGATCTA CGTGTGGTAC TCATGTCTGC CACCATCGAC 
ACTGATTTGT TTACCAATTT CTTCAGCTCT ATCCCAGATG TTGGCCCGAC ACCAGTTATC 
ACGATGCATG GAAGAACTTT TCCTGTACAA TCATTCTACC TGGAAGACAT TCTTCACAAT 
CTTCAGCATA TGCCTGAGGA ACCAGATCAA AAGAAACGGA AAAAGGGAGG ACCACCTCCA 
CCAGATGATG ATGAAGGAGA TGAAGAAGTG GATGATAAGG GTCGAAACAT GAATATTCTA 
ACAGATCCAT CAATCAACGA ATCACTGAAA ACAGCAATGT CAAGAATCTC TGAAAAAGAT 
ATTCCATTCG GTGTGATCGA AGCTATTTTG AACGATATTG CATCCCGCGG AGTGGATGGC 
GCCGTTTTAG TTTTTCTTCC CGGTTGGGCA GAAATTATGA CACTTTGCAA TCGACTTTTA 
GAGCACCAAG AATTTGGCCA AGCTAATAAA TATGAAATTC TTCCACTTCA TTCTCAATTA 
ACTAGTCAGG AACAGAGAAA AGTGTTCAAT CACTACCCAG GAAAGCGGAA GATCATTGTT 
TCAACAAATA TCGCTGAAAC AAGTATTACA ATTGATGACG TAGTCTATGT GATTGACTCT 
TGCAAAGCAA AGGAACGAAT GTATACATCG AACAACAATA TGGTTCATTT CGCAACTGTC 
TGGGCATCAA AGACAAATGT AATTCAAAGA AGAGGGCGCG CTGGTCGTGT TAGAGCTGGA 
TACGCTTTTC ATTTATGCAG TAAGATGAGA TTTGAAGCAC TGGATGATCA CGGAACTGCT 
GAAATGCTTC GGATTCCTCT TCATCAAATC GCTTTGACAA TCAAACTTCT CCGTCTCGGA 
TCCGTCGGTG AATTCCTTGG AAAGGCTCTT CAACCTCCAC CGTATGATAT GGTCGTCGAA 
AGTGAAGCAG TGTTACAAGC GATGGGAGCT CTTGATCGTA ATTTAGAATT GACAAGTCTC 
GGAAAAATGC TCGCTCGTAT GCCAATTGAG CCTGTCATTG CCAAAGTGCT TATTCTTGGA 
ACTGCTCTTG GTGCAGGATC TGTGATGTGT GACGTGGCAT CTGCAATGTC ATTTCCAACT 
CCATTCGTTC CACGAGAGAA GCATCACAGC CGATTGAGTG GTACACAGCG AAAGTTTGCT 
GGAAACAAGT TTTCTGATCA TGTTGCAATT GTCTCTGTGA TTCAAGGATA TCGTGAAGCC 
GTTCAAATGG GTGCATCTGC TGCTGAGCGC GAATTCTGTG AGCGATACTC GCTGAGTAAT 
CCTGTGCTCA AAATGACTGA TGGAGCACGT AGACAGTTGA TTGATGTACT TCGAAATCAA 
TGCAGTTTCC CTGAAGACAT TTTGTTTGAT ATCTCTGTTA ACGTCAATGG ACCAGATCGT 
GAACTTAATC TGATGCGTTC TCTTCTTGTC ATGGCTCTTT ATCCAAATGT GGCGTACTAC 
GTTGGAAAAC GGAAAGTATT AACGATAGAG CAATCGAGTG CACTAATCAA CAAGTATTCT 
ATGCTTGTTC CGATGAATAA TCGCCAAGAA ATGGATTTCC CAAGTCCTCT GCTCGTTTTC 
ACTGAAAAAG TTCGTACCAG ATGCATCTCA TGTAAACAAA TGTCAGTGAT CAGTGCAATA 
CAATTGTTAG TTTTTGGAAG TCGAAAAGTT GAATGTGTCG GAGAAGGACT TGTTCGCATT 
GATGAGACTA TTACAATTCG AATGAACGTT TCTACCGCAG CTGCTCTCAT CGGTCTTCGT 
CCATGCATCG AAGCTCTTCT CGTCAAATCT TGTGAAAATC CAGAATCTCT GGCTGGCCTC 
AACCCATCAG ATGCTGAACT TCGTCAACTT CTTCGTGACA TATCTTCCGA AGAATTCATG 
TCCGAAGCTG GACCTATCAA AGACAGTCTA CTTACAGATA ATGCGTTAAT CCAGAAAGGC 
CCTGCTCCAA ATCGTCTTGA ATATGCTGAT TGGGGACCGT CGAACAACAA TTCATCATTC 
CAAAATCAAG ATTTTCCACC AGCTGCTGGG GGCAAAGTGC ACCCTTACCG CGGTAACAGA 
CGCGGAAATC ATCCATATGC TCAAAATCGT CCCTATGCTC CTCCCAATTC CGGAATGGGC 
TATCAGAATT TCAACAACTC AGGTTACGGC GCCAGTGGCG ATTGGAACGC CGGACGTGGC 
AATTACGGCA ATGTTGGAAG CGGATATCGA GGAGCTGGCG GCGGCGGCGG TTACCGAGGT 
GGTCGTGGTG GAAGAGGTGG TGGCCGTGGC GGTGGTGGCA GAGGATGGAA TGCATCGAAC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rha-1 ( NM_001373779.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360473.1 Caenorhabditis elegans ATP-dependent RNA helicase A (rha-1), partial mRNA.

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CloneID	OCa850891
Clone ID Related Accession (Same CDS sequence)	NM_001373779.1
Accession Version	NM_001373779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	ATP-dependent RNA helicase A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on the 5' end.

ATGTCCAGAG ACGTCAAAGA ATTTCTATAT GCTTGGTTAG GAAAGAATAA GTATGGAAAT 
CCGACGTACG ATACAAAAAG TGAAACCAGG AGTGGTAGGC AGAGATTCAA ATGCGAACTT 
CGGATCACAG GATTTGGCTA CACTGCTTTC GGAAATTCCA CAAACAAAAA GGATGCTGCT 
ACCAATGCTG CTCAAGATTT CTGCCAATAC CTAGTCAGAG AAGGAAAAAT GCAACAGAGT 
GATATTCCGA CTCTAACGTC TTCGAGTCTT GAAGCTTCTT CCACGTGGCA AGATTCTGAA 
ACTGCTACAA TGTTTTGTGG TGGAGAAGAT GGCAACTCGT TCCAAGAGTC ACAGCAACCA 
ATTCCACAAA AAAGATTTCC TTGGAGTAAC AATGCATATC AACGTAACGA GGGCACACAT 
GAACAATATA TTACACAGAA AGCAGAAGAA ATTGCTGCAT CGGAAACTGT TGATCTAAAA 
TCAGAAATTC ATGGAGGATG GACGATGGAG AATTCAAAGA AAGCGCTGAA TGAGTTTCTT 
CAAAAAATGA GACTTCCACA GGTTAACTAT GGAACCAAAA TTCGAGAATC AAACACCGTT 
AAAACAATGG AGACGACTGC CCAAATCTTT GTTCCTCAAA TCAACAAAAA TCTCGTTGGA 
AAAGGTACTG GATCTAACAA GAAAGTTTCT GAAGCGGCGT GTGCAATGAA TATCGTTCGT 
CAGATGTTCC ATCTCAATAT TATGCAATCG TACAGTGGAC CAATCAAGAA GAGTAAAGTC 
AGCACGCTTC CTGAAATTGC GATTTCCATT CCAGAAGATC TCTCAACTCG AGTGACAGAA 
TATGTTCGGT CGTGCGGTCT TGAGTTGCCA GAAATCAACG AAACGAGTGC AACTCCGGAA 
GCTCCTACCT CACTGCTCAC GGATGTTAAA CTAGCGCAGT TCCCTGTTTC AGAAATTTGT 
TCCGCTTCGA ATATCTCCTG GGCACCTCCT CTTCAGAATT GGAATCCATG GAGAGCTTCG 
AATATCGATG AGGAACCATT AGCTTTTATG TCAATGGAGC AAATTAGTCA GAGAATAATG 
GAAAAAGAGG ATTTCAAGCG AGGTGAAGCA CTTGACAAAA TAACAGCTCA ACGTGGAGAA 
CTCCCTGTCG CCCAGTATCG TGAAAACATT GTACAAACCG TCGCAGAAAA TCGTGTTACA 
CTGATCAAAG GAGAAACTGG ATGTGGAAAG AGTACTCAAG TTGCTCAATT TCTACTGGAA 
AGTTTTTTGG AAAATTCTAA TGGAGCATCT TTCAATGCAG TCGTCTCTCA ACCTCGTCGT 
ATTTCTGCAA TTTCACTGGC CGAACGTGTC GCCAACGAGC GAGGAGAAGA AGTTGGAGAA 
ACATGCGGAT ATAATGTTCG ATTCGATAGT GCAACTCCTC GGCCATATGG ATCTATCATG 
TTTTGTACTG TTGGAGTCTT GCTGCGAATG ATGGAGAACG GATTACGAGG AATTTCACAT 
GTAATCATCG ATGAAATTCA CGAACGGGAT GTTGACACTG ACTTTGTTCT TATTGTGCTC 
CGCGAAATGA TTAGCACATA CCGAGATCTA CGTGTGGTAC TCATGTCTGC CACCATCGAC 
ACTGATTTGT TTACCAATTT CTTCAGCTCT ATCCCAGATG TTGGCCCGAC ACCAGTTATC 
ACGATGCATG GAAGAACTTT TCCTGTACAA TCATTCTACC TGGAAGACAT TCTTCACAAT 
CTTCAGCATA TGCCTGAGGA ACCAGATCAA AAGAAACGGA AAAAGGGAGG ACCACCTCCA 
CCAGATGATG ATGAAGGAGA TGAAGAAGTG GATGATAAGG GTCGAAACAT GAATATTCTA 
ACAGATCCAT CAATCAACGA ATCACTGAAA ACAGCAATGT CAAGAATCTC TGAAAAAGAT 
ATTCCATTCG GTGTGATCGA AGCTATTTTG AACGATATTG CATCCCGCGG AGTGGATGGC 
GCCGTTTTAG TTTTTCTTCC CGGTTGGGCA GAAATTATGA CACTTTGCAA TCGACTTTTA 
GAGCACCAAG AATTTGGCCA AGCTAATAAA TATGAAATTC TTCCACTTCA TTCTCAATTA 
ACTAGTCAGG AACAGAGAAA AGTGTTCAAT CACTACCCAG GAAAGCGGAA GATCATTGTT 
TCAACAAATA TCGCTGAAAC AAGTATTACA ATTGATGACG TAGTCTATGT GATTGACTCT 
TGCAAAGCAA AGGAACGAAT GTATACATCG AACAACAATA TGGTTCATTT CGCAACTGTC 
TGGGCATCAA AGACAAATGT AATTCAAAGA AGAGGGCGCG CTGGTCGTGT TAGAGCTGGA 
TACGCTTTTC ATTTATGCAG TAAGATGAGA TTTGAAGCAC TGGATGATCA CGGAACTGCT 
GAAATGCTTC GGATTCCTCT TCATCAAATC GCTTTGACAA TCAAACTTCT CCGTCTCGGA 
TCCGTCGGTG AATTCCTTGG AAAGGCTCTT CAACCTCCAC CGTATGATAT GGTCGTCGAA 
AGTGAAGCAG TGTTACAAGC GATGGGAGCT CTTGATCGTA ATTTAGAATT GACAAGTCTC 
GGAAAAATGC TCGCTCGTAT GCCAATTGAG CCTGTCATTG CCAAAGTGCT TATTCTTGGA 
ACTGCTCTTG GTGCAGGATC TGTGATGTGT GACGTGGCAT CTGCAATGTC ATTTCCAACT 
CCATTCGTTC CACGAGAGAA GCATCACAGC CGATTGAGTG GTACACAGCG AAAGTTTGCT 
GGAAACAAGT TTTCTGATCA TGTTGCAATT GTCTCTGTGA TTCAAGGATA TCGTGAAGCC 
GTTCAAATGG GTGCATCTGC TGCTGAGCGC GAATTCTGTG AGCGATACTC GCTGAGTAAT 
CCTGTGCTCA AAATGACTGA TGGAGCACGT AGACAGTTGA TTGATGTACT TCGAAATCAA 
TGCAGTTTCC CTGAAGACAT TTTGTTTGAT ATCTCTGTTA ACGTCAATGG ACCAGATCGT 
GAACTTAATC TGATGCGTTC TCTTCTTGTC ATGGCTCTTT ATCCAAATGT GGCGTACTAC 
GTTGGAAAAC GGAAAGTATT AACGATAGAG CAATCGAGTG CACTAATCAA CAAGTATTCT 
ATGCTTGTTC CGATGAATAA TCGCCAAGAA ATGGATTTCC CAAGTCCTCT GCTCGTTTTC 
ACTGAAAAAG TTCGTACCAG ATGCATCTCA TGTAAACAAA TGTCAGTGAT CAGTGCAATA 
CAATTGTTAG TTTTTGGAAG TCGAAAAGTT GAATGTGTCG GAGAAGGACT TGTTCGCATT 
GATGAGACTA TTACAATTCG AATGAACGTT TCTACCGCAG CTGCTCTCAT CGGTCTTCGT 
CCATGCATCG AAGCTCTTCT CGTCAAATCT TGTGAAAATC CAGAATCTCT GGCTGGCCTC 
AACCCATCAG ATGCTGAACT TCGTCAACTT CTTCGTGACA TATCTTCCGA AGAATTCATG 
TCCGAAGCTG GACCTATCAA AGACAGTCTA CTTACAGATA ATGCGTTAAT CCAGAAAGGC 
CCTGCTCCAA ATCGTCTTGA ATATGCTGAT TGGGGACCGT CGAACAACAA TTCATCATTC 
CAAAATCAAG ATTTTCCACC AGGTTACGGC GCCAGTGGCG ATTGGAACGC CGGACGTGGC 
AATTACGGCA ATGTTGGAAG CGGATATCGA GGAGCTGGCG GCGGCGGCGG TTACCGAGGT 
GGTCGTGGTG GAAGAGGTGG TGGCCGTGGC GGTGGTGGCA GAGGATGGAA TGCATCGAAC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360473.1
CDS	1..3786
Translation	MSRDVKEFLYAWLGKNKYGNPTYDTKSETRSGRQRFKCELRITGFGYTAFGNSTNKKDAATNAAQDFCQYLVREGKMQQSDIPTLTSSSLEASSTWQDSETATMFCGGEDGNSFQESQQPIPQKRFPWSNNAYQRNEGTHEQYITQKAEEIAASETVDLKSEIHGGWTMENSKKALNEFLQKMRLPQVNYGTKIRESNTVKTMETTAQIFVPQINKNLVGKGTGSNKKVSEAACAMNIVRQMFHLNIMQSYSGPIKKSKVSTLPEIAISIPEDLSTRVTEYVRSCGLELPEINETSATPEAPTSLLTDVKLAQFPVSEICSASNISWAPPLQNWNPWRASNIDEEPLAFMSMEQISQRIMEKEDFKRGEALDKITAQRGELPVAQYRENIVQTVAENRVTLIKGETGCGKSTQVAQFLLESFLENSNGASFNAVVSQPRRISAISLAERVANERGEEVGETCGYNVRFDSATPRPYGSIMFCTVGVLLRMMENGLRGISHVIIDEIHERDVDTDFVLIVLREMISTYRDLRVVLMSATIDTDLFTNFFSSIPDVGPTPVITMHGRTFPVQSFYLEDILHNLQHMPEEPDQKKRKKGGPPPPDDDEGDEEVDDKGRNMNILTDPSINESLKTAMSRISEKDIPFGVIEAILNDIASRGVDGAVLVFLPGWAEIMTLCNRLLEHQEFGQANKYEILPLHSQLTSQEQRKVFNHYPGKRKIIVSTNIAETSITIDDVVYVIDSCKAKERMYTSNNNMVHFATVWASKTNVIQRRGRAGRVRAGYAFHLCSKMRFEALDDHGTAEMLRIPLHQIALTIKLLRLGSVGEFLGKALQPPPYDMVVESEAVLQAMGALDRNLELTSLGKMLARMPIEPVIAKVLILGTALGAGSVMCDVASAMSFPTPFVPREKHHSRLSGTQRKFAGNKFSDHVAIVSVIQGYREAVQMGASAAEREFCERYSLSNPVLKMTDGARRQLIDVLRNQCSFPEDILFDISVNVNGPDRELNLMRSLLVMALYPNVAYYVGKRKVLTIEQSSALINKYSMLVPMNNRQEMDFPSPLLVFTEKVRTRCISCKQMSVISAIQLLVFGSRKVECVGEGLVRIDETITIRMNVSTAAALIGLRPCIEALLVKSCENPESLAGLNPSDAELRQLLRDISSEEFMSEAGPIKDSLLTDNALIQKGPAPNRLEYADWGPSNNNSSFQNQDFPPGYGASGDWNAGRGNYGNVGSGYRGAGGGGGYRGGRGGRGGGRGGGGRGWNASNW

Target ORF information:

RefSeq Version	NM_001373779.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans ATP-dependent RNA helicase A (rha-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373779.1

ORF Insert Sequence:

ATGTCCAGAG ACGTCAAAGA ATTTCTATAT GCTTGGTTAG GAAAGAATAA GTATGGAAAT 
CCGACGTACG ATACAAAAAG TGAAACCAGG AGTGGTAGGC AGAGATTCAA ATGCGAACTT 
CGGATCACAG GATTTGGCTA CACTGCTTTC GGAAATTCCA CAAACAAAAA GGATGCTGCT 
ACCAATGCTG CTCAAGATTT CTGCCAATAC CTAGTCAGAG AAGGAAAAAT GCAACAGAGT 
GATATTCCGA CTCTAACGTC TTCGAGTCTT GAAGCTTCTT CCACGTGGCA AGATTCTGAA 
ACTGCTACAA TGTTTTGTGG TGGAGAAGAT GGCAACTCGT TCCAAGAGTC ACAGCAACCA 
ATTCCACAAA AAAGATTTCC TTGGAGTAAC AATGCATATC AACGTAACGA GGGCACACAT 
GAACAATATA TTACACAGAA AGCAGAAGAA ATTGCTGCAT CGGAAACTGT TGATCTAAAA 
TCAGAAATTC ATGGAGGATG GACGATGGAG AATTCAAAGA AAGCGCTGAA TGAGTTTCTT 
CAAAAAATGA GACTTCCACA GGTTAACTAT GGAACCAAAA TTCGAGAATC AAACACCGTT 
AAAACAATGG AGACGACTGC CCAAATCTTT GTTCCTCAAA TCAACAAAAA TCTCGTTGGA 
AAAGGTACTG GATCTAACAA GAAAGTTTCT GAAGCGGCGT GTGCAATGAA TATCGTTCGT 
CAGATGTTCC ATCTCAATAT TATGCAATCG TACAGTGGAC CAATCAAGAA GAGTAAAGTC 
AGCACGCTTC CTGAAATTGC GATTTCCATT CCAGAAGATC TCTCAACTCG AGTGACAGAA 
TATGTTCGGT CGTGCGGTCT TGAGTTGCCA GAAATCAACG AAACGAGTGC AACTCCGGAA 
GCTCCTACCT CACTGCTCAC GGATGTTAAA CTAGCGCAGT TCCCTGTTTC AGAAATTTGT 
TCCGCTTCGA ATATCTCCTG GGCACCTCCT CTTCAGAATT GGAATCCATG GAGAGCTTCG 
AATATCGATG AGGAACCATT AGCTTTTATG TCAATGGAGC AAATTAGTCA GAGAATAATG 
GAAAAAGAGG ATTTCAAGCG AGGTGAAGCA CTTGACAAAA TAACAGCTCA ACGTGGAGAA 
CTCCCTGTCG CCCAGTATCG TGAAAACATT GTACAAACCG TCGCAGAAAA TCGTGTTACA 
CTGATCAAAG GAGAAACTGG ATGTGGAAAG AGTACTCAAG TTGCTCAATT TCTACTGGAA 
AGTTTTTTGG AAAATTCTAA TGGAGCATCT TTCAATGCAG TCGTCTCTCA ACCTCGTCGT 
ATTTCTGCAA TTTCACTGGC CGAACGTGTC GCCAACGAGC GAGGAGAAGA AGTTGGAGAA 
ACATGCGGAT ATAATGTTCG ATTCGATAGT GCAACTCCTC GGCCATATGG ATCTATCATG 
TTTTGTACTG TTGGAGTCTT GCTGCGAATG ATGGAGAACG GATTACGAGG AATTTCACAT 
GTAATCATCG ATGAAATTCA CGAACGGGAT GTTGACACTG ACTTTGTTCT TATTGTGCTC 
CGCGAAATGA TTAGCACATA CCGAGATCTA CGTGTGGTAC TCATGTCTGC CACCATCGAC 
ACTGATTTGT TTACCAATTT CTTCAGCTCT ATCCCAGATG TTGGCCCGAC ACCAGTTATC 
ACGATGCATG GAAGAACTTT TCCTGTACAA TCATTCTACC TGGAAGACAT TCTTCACAAT 
CTTCAGCATA TGCCTGAGGA ACCAGATCAA AAGAAACGGA AAAAGGGAGG ACCACCTCCA 
CCAGATGATG ATGAAGGAGA TGAAGAAGTG GATGATAAGG GTCGAAACAT GAATATTCTA 
ACAGATCCAT CAATCAACGA ATCACTGAAA ACAGCAATGT CAAGAATCTC TGAAAAAGAT 
ATTCCATTCG GTGTGATCGA AGCTATTTTG AACGATATTG CATCCCGCGG AGTGGATGGC 
GCCGTTTTAG TTTTTCTTCC CGGTTGGGCA GAAATTATGA CACTTTGCAA TCGACTTTTA 
GAGCACCAAG AATTTGGCCA AGCTAATAAA TATGAAATTC TTCCACTTCA TTCTCAATTA 
ACTAGTCAGG AACAGAGAAA AGTGTTCAAT CACTACCCAG GAAAGCGGAA GATCATTGTT 
TCAACAAATA TCGCTGAAAC AAGTATTACA ATTGATGACG TAGTCTATGT GATTGACTCT 
TGCAAAGCAA AGGAACGAAT GTATACATCG AACAACAATA TGGTTCATTT CGCAACTGTC 
TGGGCATCAA AGACAAATGT AATTCAAAGA AGAGGGCGCG CTGGTCGTGT TAGAGCTGGA 
TACGCTTTTC ATTTATGCAG TAAGATGAGA TTTGAAGCAC TGGATGATCA CGGAACTGCT 
GAAATGCTTC GGATTCCTCT TCATCAAATC GCTTTGACAA TCAAACTTCT CCGTCTCGGA 
TCCGTCGGTG AATTCCTTGG AAAGGCTCTT CAACCTCCAC CGTATGATAT GGTCGTCGAA 
AGTGAAGCAG TGTTACAAGC GATGGGAGCT CTTGATCGTA ATTTAGAATT GACAAGTCTC 
GGAAAAATGC TCGCTCGTAT GCCAATTGAG CCTGTCATTG CCAAAGTGCT TATTCTTGGA 
ACTGCTCTTG GTGCAGGATC TGTGATGTGT GACGTGGCAT CTGCAATGTC ATTTCCAACT 
CCATTCGTTC CACGAGAGAA GCATCACAGC CGATTGAGTG GTACACAGCG AAAGTTTGCT 
GGAAACAAGT TTTCTGATCA TGTTGCAATT GTCTCTGTGA TTCAAGGATA TCGTGAAGCC 
GTTCAAATGG GTGCATCTGC TGCTGAGCGC GAATTCTGTG AGCGATACTC GCTGAGTAAT 
CCTGTGCTCA AAATGACTGA TGGAGCACGT AGACAGTTGA TTGATGTACT TCGAAATCAA 
TGCAGTTTCC CTGAAGACAT TTTGTTTGAT ATCTCTGTTA ACGTCAATGG ACCAGATCGT 
GAACTTAATC TGATGCGTTC TCTTCTTGTC ATGGCTCTTT ATCCAAATGT GGCGTACTAC 
GTTGGAAAAC GGAAAGTATT AACGATAGAG CAATCGAGTG CACTAATCAA CAAGTATTCT 
ATGCTTGTTC CGATGAATAA TCGCCAAGAA ATGGATTTCC CAAGTCCTCT GCTCGTTTTC 
ACTGAAAAAG TTCGTACCAG ATGCATCTCA TGTAAACAAA TGTCAGTGAT CAGTGCAATA 
CAATTGTTAG TTTTTGGAAG TCGAAAAGTT GAATGTGTCG GAGAAGGACT TGTTCGCATT 
GATGAGACTA TTACAATTCG AATGAACGTT TCTACCGCAG CTGCTCTCAT CGGTCTTCGT 
CCATGCATCG AAGCTCTTCT CGTCAAATCT TGTGAAAATC CAGAATCTCT GGCTGGCCTC 
AACCCATCAG ATGCTGAACT TCGTCAACTT CTTCGTGACA TATCTTCCGA AGAATTCATG 
TCCGAAGCTG GACCTATCAA AGACAGTCTA CTTACAGATA ATGCGTTAAT CCAGAAAGGC 
CCTGCTCCAA ATCGTCTTGA ATATGCTGAT TGGGGACCGT CGAACAACAA TTCATCATTC 
CAAAATCAAG ATTTTCCACC AGGTTACGGC GCCAGTGGCG ATTGGAACGC CGGACGTGGC 
AATTACGGCA ATGTTGGAAG CGGATATCGA GGAGCTGGCG GCGGCGGCGG TTACCGAGGT 
GGTCGTGGTG GAAGAGGTGG TGGCCGTGGC GGTGGTGGCA GAGGATGGAA TGCATCGAAC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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rha-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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