kcc-3 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	kcc-3
Entrez Gene ID	174212
Full Name	K+/Cl-Cotransporter
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001351920.2	NP_001338797.1	Potassium/chloride cotransporter 3
NM_001351919.2	NP_001338828.1	Potassium/chloride cotransporter 3
NM_063154.4	NP_495555.2	Sodium/chloride cotransporter 3
NM_001351920.1	NP_001338797.1	Potassium/chloride cotransporter 3
NM_001351919.1	NP_001338828.1	Potassium/chloride cotransporter 3

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	kcc-1	NP_501141.1
Caenorhabditis elegans (roundworm)	kcc-3	NP_495555.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study finds that an AMsh glia-expressed K/Cl co-transporter, KCC-3, regulates AFD neuronal receptive endings shape and thermosensory behavior by controlling Cl-levels surrounding AFD neuronal receptive endings; Cl-ions directly inhibit the AFD neuron-specific receptor guanylyl cyclase, GCY-8 by binding to the conserved S(x)nGPxC motif in its extracellular domain.
Title: A Glial K/Cl Transporter Controls Neuronal Receptive Ending Shape by Chloride Inhibition of an rGC.

The following kcc-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kcc-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa338527	NM_063154.4 Latest version!	Caenorhabditis elegans Sodium/chloride cotransporter 3 (kcc-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa529777	NM_001351920.2 Latest version!	Caenorhabditis elegans Potassium/chloride cotransporter 3 (kcc-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa529778	NM_001351919.2 Latest version!	Caenorhabditis elegans Potassium/chloride cotransporter 3 (kcc-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa529777	NM_001351920.1	Caenorhabditis elegans Potassium/chloride cotransporter 3 (kcc-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa529778	NM_001351919.1	Caenorhabditis elegans Potassium/chloride cotransporter 3 (kcc-3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kcc-3 ( NM_063154.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495555.2 Caenorhabditis elegans Sodium/chloride cotransporter 3 (kcc-3), partial mRNA.

Price & Availability↑

CloneID	OCa338527
Clone ID Related Accession (Same CDS sequence)	NM_063154.4
Accession Version	NM_063154.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-12
Organism	Caenorhabditis elegans
Product	Sodium/chloride cotransporter 3
Comment	REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_063154.3. COMPLETENESS: incomplete on both ends.

ATGTCGAATG CAAGGCGCCG TTTCAGTACG GTGACGCAGA TAAATACAGA AGGCCTTCAA 
GCTATGGGAA AAGGCGGGGG TCGAATGGAA ACTGTCGGAG AGGATGGTAT CCCTGCGGAT 
TATAAAGGAA ATCGAAAATT CACGACAAGT CTCGGTCACC TGGCATTATA CAAGGAGGAT 
GAGGGGATTG GGACACAAGC TTCTTTTATT TCGGGTTATA CAACGCCCGG TCCGAAAGAA 
AGAGCAACTT CTGAGCATGT GAAGGCCAAC TTGGGAGTGA TGCTTGGAGT TTATTTACCC 
ACAATTCAAC ATATTCTCGG AGTTACCATG TTTATCCGTC TTTTTTGGGT CGTTGGAATG 
TCTGGCGTTG CTTGGACAAT GGCCCTACTC GCCATTTGTT GTTTATCGAC TCTTTTGACC 
TCAATCTCAT TATCAGCAGT TGCCACAAAC GGAGTCGTTG AATCTGGAGG AGCATATTTC 
ATTATTAGCA GAAATCTTGG AGCTGAATTT GGATCAGCTG TTGGTATTTT GTTTTACTTG 
GCAAACACAG TGGCTGCATC GATGTACATT GTTGGCGGTG TTGAAGTTAT TCTCATGTAT 
TTGTGGCCTG AAATGGCAAT TGGTGGAGCT GATGCACTTC ATGATACTGA AATGTTTGGA 
AGTCTTTATA ATAATCTTCG TCTCTACGGA ACCGTTTTTC TTCTCATTCA AGCTCTCATT 
GTGGCAATGG GTGTCAAATT TGTGCAACTA CTTGCTCCAG TGTCTCTGAT GTGTGTTATA 
TTGGCAATTG CTGCTTGTAT CGGAGGTGGA ATTGAAAAAC AGATTACAAT GGAAGGAATG 
AAAGTATGTG CCATTGACAA TCATTTGTTG CAATCTTCAA TTGTCACTCA CCCAATTCAT 
AAAAATCAGA CTAGTTGGTT TAATGAAACT GTTGATTTCT GCAATCTATG TGATAAATCT 
CTTTATCTTG AAAGTGTTTT CTGTGCAAAT GTGAATAATG ACGAAGCTTC AGCAGAAGAT 
GATGTTTTTT GTACTCACTA TACTTCAAAA AAGATGACAT GCCAATTAGC ATTCCCTGGT 
TTCAATATGA AAACTCTAAA CGACAATATG TGGCCCGAGT ACATGGAAAA ATCAGAAGTG 
GTACCTGGTG TGAGAGGAAA AGAAACTGCT GAAGTTGTAC AAGATGAATC TTCAACATTC 
TTCATGCTTA TGGCTATTTA TTTCCCAGCT GTGACTGGAA TCTTCACTGG AACAAATATG 
AGCGGAGATT TGAGAGATCC ACAAAGAAGT ATCCCTGTTG GAACAATTGC TGCAACACTT 
ACAACTTCAG CAATCTACTA CATACTTGCA ATTTTATTTG GTGGATCGAT AACAAGAAGT 
GTGTTACGTG ACAAATTCGG TCGATCAATT GGAAATACAA TGGTTGTTGC TGCCCTCTCA 
TGGCCACATC CTGCTGTTGT AACTGTTGGA GCATTCTTGT CTACTTTTGG TGCTGCTCTT 
CAATGCCTCT GCAGTGCGCC CCGTCTATTA CAATCGATCG CCAAGGATGA CGTAATCCCG 
ATTCTCGCCC CATTCGCCCG TGTTACAAAA AACAATGAAC CTTTTCTTGG ATTGGTTCTC 
ACTGTCATCA TCGCAGAATG TGGAATCCTT CTTGGAGCTG TGGATAAGAT TGCCGAAGTT 
CTTGATTTCT TTTTCCTTAT GACTCTATCA CTTCTTGGAG CGGCTCTATG CTTCTTCATT 
ATGTTTGCTT CAAGTGTTCC ACTGGCATGT ATTGCTTGTA CCGCAACTGC AGTCATCTAT 
AAATATGTGG AATGGAAAGG AGCCAAGAAA GAATGGGGAG ATGGAATGAG AGGTTTGGCT 
CTGACAACTG CACAATACAG CCTGCTAAAA GTCGAGGACA AAGATCCACA TCCAAAGAAT 
TGGAGACCGC AGGTTTTGAT TCTTCTCACA AGTCAATGGT CAAAGGAAAT GATTGATAGA 
AGAGCTGTGT CAATGCTAAA TTTGGGGGCA CAGTTGAAAG CTGGACGAGG ACTTGCTATT 
GCTTGTGCTT TTTTGAAAGG TTCAGTTGAT TCCCAGAAAG ATAAAAACCG TGCAAGAGAC 
GTTAAAACCA CTCTTGTAAA AGATATGTCA AGTGTTCGTC TTCGTGGATT TGCCAAAACC 
ATGTTCTATA ACAATCATCA AATTAATGGA ACTATATCTG GACTTTATCA GTCAATTGGA 
ATTGGAGGAT TGAGACCGAA CACTATTCTA CTGAATTGGC CGAATGAGAA GAATCCCGAT 
GAGCTTGTTC TTTTTGCTGA GGAAATTATT CATGGAGCTG CAAATGATAA CTGTTTGATC 
GTCACCAAAG GAATCACAGA TTTCCCTGAA TACTCTGAAC GTCTAACTGG TTTTATTGAT 
ATTTGGTGGA TTGTACAAGA TGGAGGAATT CTTATGCTTA TTGCTTATCT TCTTCGTCAG 
CATAAAGTAT GGAAAGGTTG CACACTTCGA ATTTTTGCAG TCAGTGAACA AGATTCTACG 
AAATCAGAAG ATATGAAAGC AGGACTTCAG AAATATATTT ATATGCTGAG AATTGATGCC 
GAGCTTTTCA TTGTTGATCT TCTTGATATG GAAGTGTCAG ATGAAGTAGT TGAAAAAGCA 
GCAGAAGTCG AAAGAAAACA GAAAGAAAGA GAAGAAATGA GAAGAAGTAA AAGTGGATAT 
TTGAATGATG GTTTTATGGA AGACAATGGA AAACCTCGGC AAGTGATGAT GCGTCATAGC 
GATTCAGCAA GAAGTTTTAG TCCACAGCCA GGTGCACATA CTTCCATCAA CTTGGATGAG 
ACAGAAACAT CATTTACCGA AAGCCTATTT GATGATTTCT ACCGTTCGGG AACTCCCAAT 
GAAGATCTCG AAGGTGCAAT GAAACTGAAC ATTCACAAAA TGAATACATC CGTTCGTCTA 
AATCGTGTTA TTCGAGAAAA TTCTCCAGAC TCTCAGTTGA TCTTATTGAA TCTACCATCT 
CCTCCAAGAA ACCGTCTTGC TTTCAACAAT TCTTATATGA CATATTTGGA CGTGTTGACT 
GAAGATTTGC CACGTGTACT TTTCATTGGT GGAAGTGGCC GTGAAGTTAT TACAATCGAC 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495555.2
CDS	1..3126
Translation	MSNARRRFSTVTQINTEGLQAMGKGGGRMETVGEDGIPADYKGNRKFTTSLGHLALYKEDEGIGTQASFISGYTTPGPKERATSEHVKANLGVMLGVYLPTIQHILGVTMFIRLFWVVGMSGVAWTMALLAICCLSTLLTSISLSAVATNGVVESGGAYFIISRNLGAEFGSAVGILFYLANTVAASMYIVGGVEVILMYLWPEMAIGGADALHDTEMFGSLYNNLRLYGTVFLLIQALIVAMGVKFVQLLAPVSLMCVILAIAACIGGGIEKQITMEGMKVCAIDNHLLQSSIVTHPIHKNQTSWFNETVDFCNLCDKSLYLESVFCANVNNDEASAEDDVFCTHYTSKKMTCQLAFPGFNMKTLNDNMWPEYMEKSEVVPGVRGKETAEVVQDESSTFFMLMAIYFPAVTGIFTGTNMSGDLRDPQRSIPVGTIAATLTTSAIYYILAILFGGSITRSVLRDKFGRSIGNTMVVAALSWPHPAVVTVGAFLSTFGAALQCLCSAPRLLQSIAKDDVIPILAPFARVTKNNEPFLGLVLTVIIAECGILLGAVDKIAEVLDFFFLMTLSLLGAALCFFIMFASSVPLACIACTATAVIYKYVEWKGAKKEWGDGMRGLALTTAQYSLLKVEDKDPHPKNWRPQVLILLTSQWSKEMIDRRAVSMLNLGAQLKAGRGLAIACAFLKGSVDSQKDKNRARDVKTTLVKDMSSVRLRGFAKTMFYNNHQINGTISGLYQSIGIGGLRPNTILLNWPNEKNPDELVLFAEEIIHGAANDNCLIVTKGITDFPEYSERLTGFIDIWWIVQDGGILMLIAYLLRQHKVWKGCTLRIFAVSEQDSTKSEDMKAGLQKYIYMLRIDAELFIVDLLDMEVSDEVVEKAAEVERKQKEREEMRRSKSGYLNDGFMEDNGKPRQVMMRHSDSARSFSPQPGAHTSINLDETETSFTESLFDDFYRSGTPNEDLEGAMKLNIHKMNTSVRLNRVIRENSPDSQLILLNLPSPPRNRLAFNNSYMTYLDVLTEDLPRVLFIGGSGREVITIDS

Target ORF information:

RefSeq Version	NM_063154.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Sodium/chloride cotransporter 3 (kcc-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063154.4

ORF Insert Sequence:

ATGTCGAATG CAAGGCGCCG TTTCAGTACG GTGACGCAGA TAAATACAGA AGGCCTTCAA 
GCTATGGGAA AAGGCGGGGG TCGAATGGAA ACTGTCGGAG AGGATGGTAT CCCTGCGGAT 
TATAAAGGAA ATCGAAAATT CACGACAAGT CTCGGTCACC TGGCATTATA CAAGGAGGAT 
GAGGGGATTG GGACACAAGC TTCTTTTATT TCGGGTTATA CAACGCCCGG TCCGAAAGAA 
AGAGCAACTT CTGAGCATGT GAAGGCCAAC TTGGGAGTGA TGCTTGGAGT TTATTTACCC 
ACAATTCAAC ATATTCTCGG AGTTACCATG TTTATCCGTC TTTTTTGGGT CGTTGGAATG 
TCTGGCGTTG CTTGGACAAT GGCCCTACTC GCCATTTGTT GTTTATCGAC TCTTTTGACC 
TCAATCTCAT TATCAGCAGT TGCCACAAAC GGAGTCGTTG AATCTGGAGG AGCATATTTC 
ATTATTAGCA GAAATCTTGG AGCTGAATTT GGATCAGCTG TTGGTATTTT GTTTTACTTG 
GCAAACACAG TGGCTGCATC GATGTACATT GTTGGCGGTG TTGAAGTTAT TCTCATGTAT 
TTGTGGCCTG AAATGGCAAT TGGTGGAGCT GATGCACTTC ATGATACTGA AATGTTTGGA 
AGTCTTTATA ATAATCTTCG TCTCTACGGA ACCGTTTTTC TTCTCATTCA AGCTCTCATT 
GTGGCAATGG GTGTCAAATT TGTGCAACTA CTTGCTCCAG TGTCTCTGAT GTGTGTTATA 
TTGGCAATTG CTGCTTGTAT CGGAGGTGGA ATTGAAAAAC AGATTACAAT GGAAGGAATG 
AAAGTATGTG CCATTGACAA TCATTTGTTG CAATCTTCAA TTGTCACTCA CCCAATTCAT 
AAAAATCAGA CTAGTTGGTT TAATGAAACT GTTGATTTCT GCAATCTATG TGATAAATCT 
CTTTATCTTG AAAGTGTTTT CTGTGCAAAT GTGAATAATG ACGAAGCTTC AGCAGAAGAT 
GATGTTTTTT GTACTCACTA TACTTCAAAA AAGATGACAT GCCAATTAGC ATTCCCTGGT 
TTCAATATGA AAACTCTAAA CGACAATATG TGGCCCGAGT ACATGGAAAA ATCAGAAGTG 
GTACCTGGTG TGAGAGGAAA AGAAACTGCT GAAGTTGTAC AAGATGAATC TTCAACATTC 
TTCATGCTTA TGGCTATTTA TTTCCCAGCT GTGACTGGAA TCTTCACTGG AACAAATATG 
AGCGGAGATT TGAGAGATCC ACAAAGAAGT ATCCCTGTTG GAACAATTGC TGCAACACTT 
ACAACTTCAG CAATCTACTA CATACTTGCA ATTTTATTTG GTGGATCGAT AACAAGAAGT 
GTGTTACGTG ACAAATTCGG TCGATCAATT GGAAATACAA TGGTTGTTGC TGCCCTCTCA 
TGGCCACATC CTGCTGTTGT AACTGTTGGA GCATTCTTGT CTACTTTTGG TGCTGCTCTT 
CAATGCCTCT GCAGTGCGCC CCGTCTATTA CAATCGATCG CCAAGGATGA CGTAATCCCG 
ATTCTCGCCC CATTCGCCCG TGTTACAAAA AACAATGAAC CTTTTCTTGG ATTGGTTCTC 
ACTGTCATCA TCGCAGAATG TGGAATCCTT CTTGGAGCTG TGGATAAGAT TGCCGAAGTT 
CTTGATTTCT TTTTCCTTAT GACTCTATCA CTTCTTGGAG CGGCTCTATG CTTCTTCATT 
ATGTTTGCTT CAAGTGTTCC ACTGGCATGT ATTGCTTGTA CCGCAACTGC AGTCATCTAT 
AAATATGTGG AATGGAAAGG AGCCAAGAAA GAATGGGGAG ATGGAATGAG AGGTTTGGCT 
CTGACAACTG CACAATACAG CCTGCTAAAA GTCGAGGACA AAGATCCACA TCCAAAGAAT 
TGGAGACCGC AGGTTTTGAT TCTTCTCACA AGTCAATGGT CAAAGGAAAT GATTGATAGA 
AGAGCTGTGT CAATGCTAAA TTTGGGGGCA CAGTTGAAAG CTGGACGAGG ACTTGCTATT 
GCTTGTGCTT TTTTGAAAGG TTCAGTTGAT TCCCAGAAAG ATAAAAACCG TGCAAGAGAC 
GTTAAAACCA CTCTTGTAAA AGATATGTCA AGTGTTCGTC TTCGTGGATT TGCCAAAACC 
ATGTTCTATA ACAATCATCA AATTAATGGA ACTATATCTG GACTTTATCA GTCAATTGGA 
ATTGGAGGAT TGAGACCGAA CACTATTCTA CTGAATTGGC CGAATGAGAA GAATCCCGAT 
GAGCTTGTTC TTTTTGCTGA GGAAATTATT CATGGAGCTG CAAATGATAA CTGTTTGATC 
GTCACCAAAG GAATCACAGA TTTCCCTGAA TACTCTGAAC GTCTAACTGG TTTTATTGAT 
ATTTGGTGGA TTGTACAAGA TGGAGGAATT CTTATGCTTA TTGCTTATCT TCTTCGTCAG 
CATAAAGTAT GGAAAGGTTG CACACTTCGA ATTTTTGCAG TCAGTGAACA AGATTCTACG 
AAATCAGAAG ATATGAAAGC AGGACTTCAG AAATATATTT ATATGCTGAG AATTGATGCC 
GAGCTTTTCA TTGTTGATCT TCTTGATATG GAAGTGTCAG ATGAAGTAGT TGAAAAAGCA 
GCAGAAGTCG AAAGAAAACA GAAAGAAAGA GAAGAAATGA GAAGAAGTAA AAGTGGATAT 
TTGAATGATG GTTTTATGGA AGACAATGGA AAACCTCGGC AAGTGATGAT GCGTCATAGC 
GATTCAGCAA GAAGTTTTAG TCCACAGCCA GGTGCACATA CTTCCATCAA CTTGGATGAG 
ACAGAAACAT CATTTACCGA AAGCCTATTT GATGATTTCT ACCGTTCGGG AACTCCCAAT 
GAAGATCTCG AAGGTGCAAT GAAACTGAAC ATTCACAAAA TGAATACATC CGTTCGTCTA 
AATCGTGTTA TTCGAGAAAA TTCTCCAGAC TCTCAGTTGA TCTTATTGAA TCTACCATCT 
CCTCCAAGAA ACCGTCTTGC TTTCAACAAT TCTTATATGA CATATTTGGA CGTGTTGACT 
GAAGATTTGC CACGTGTACT TTTCATTGGT GGAAGTGGCC GTGAAGTTAT TACAATCGAC 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kcc-3 ( NM_001351920.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001338797.1 Caenorhabditis elegans Potassium/chloride cotransporter 3 (kcc-3), mRNA.

Price & Availability↑

CloneID	OCa529777
Clone ID Related Accession (Same CDS sequence)	NM_001351920.2 , NM_001351920.1
Accession Version	NM_001351920.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Potassium/chloride cotransporter 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001351920.1.

ATGTCGAATG CAAGGCGCCG TTTCAGTACG GTGACGCAGA TAAATACAGA AGGCCTTCAA 
GCTATGGGAA AAGGCGGGGG TCGAATGGAA ACTGTCGGAG AGGATGGTAT CCCTGCGGAT 
TATAAAGGAA ATCGAAAATT CACGACAAGT CTCGGTCACC TGGCATTATA CAAGGAGGAT 
GAGGGGATTG GGACACAAGC TTCTTTTATT TCGGGTTATA CAACGCCCGG TCCGAAAGAA 
AGAGCAACTT CTGAGCATGT GAAGGCCAAC TTGGGAGTGA TGCTTGGAGT TTATTTACCC 
ACAATTCAAC ATATTCTCGG AGTTACCATG TTTATCCGTC TTTTTTGGGT CGTTGGAATG 
TCTGGCGTTG CTTGGACAAT GGCCCTACTC GCCATTTGTT GTTTATCGAC TCTTTTGACC 
TCAATCTCAT TATCAGCAGT TGCCACAAAC GGAGTCGTTG AATCTGGAGG AGCATATTTC 
ATTATTAGCA GAAATCTTGG AGCTGAATTT GGATCAGCTG TTGGTATTTT GTTTTACTTG 
GCAAACACAG TGGCTGCATC GATGTACATT GTTGGCGGTG TTGAAGTTAT TCTCATGTAT 
TTGTGGCCTG AAATGGCAAT TGGTGGAGCT GATGCACTTC ATGATACTGA AATGTTTGGA 
AGTCTTTATA ATAATCTTCG TCTCTACGGA ACCGTTTTTC TTCTCATTCA AGCTCTCATT 
GTGGCAATGG GTGTCAAATT TGTGCAACTA CTTGCTCCAG TGTCTCTGAT GTGTGTTATA 
TTGGCAATTG CTGCTTGTAT CGGAGGTGGA ATTGAAAAAC AGATTACAAT GGAAGGAATG 
AAAGTATGTG CCATTGACAA TCATTTGTTG CAATCTTCAA TTGTCACTCA CCCAATTCAT 
AAAAATCAGA CTAGTTGGTT TAATGAAACT GTTGATTTCT GCAATCTATG TGATAAATCT 
CTTTATCTTG AAAGTGTTTT CTGTGCAAAT GTGAATAATG ACGAAGCTTC AGCAGAAGAT 
GATGTTTTTT GTACTCACTA TACTTCAAAA AAGATGACAT GCCAATTAGC ATTCCCTGGT 
TTCAATATGA AAACTCTAAA CGACAATATG TGGCCCGAGT ACATGGAAAA ATCAGAAGTG 
GTACCTGGTG TGAGAGGAAA AGAAACTGCT GAAGTTGTAC AAGATGAATC TTCAACATTC 
TTCATGCTTA TGGCTATTTA TTTCCCAGCT GTGACTGGAA TCTTCACTGG AACAAATATG 
AGCGGAGATT TGAGAGATCC ACAAAGAAGT ATCCCTGTTG GAACAATTGC TGCAACACTT 
ACAACTTCAG CAATCTACTA CATACTTGCA ATTTTATTTG GTGGATCGAT AACAAGAAGT 
GTGTTACGTG ACAAATTCGG TCGATCAATT GGAAATACAA TGGTTGTTGC TGCCCTCTCA 
TGGCCACATC CTGCTGTTGT AACTGTTGGA GCATTCTTGT CTACTTTTGG TGCTGCTCTT 
CAATGCCTCT GCAGTGCGCC CCGTCTATTA CAATCGATCG CCAAGGATGA CGTAATCCCG 
ATTCTCGCCC CATTCGCCCG TGTTACAAAA AACAATGAAC CTTTTCTTGG ATTGGTTCTC 
ACTGTCATCA TCGCAGAATG TGGAATCCTT CTTGGAGCTG TGGATAAGAT TGCCGAAGTT 
CTTGATTTCT TTTTCCTTAT GTGTTATGCA TTTGTGAATT TGATAGCAGT TCTTCATTCC 
GTCTTGAAGT CACCAAATTG GCGACCCAGA TTTAAATATT TTCACTGGAC TCTATCACTT 
CTTGGAGCGG CTCTATGCTT CTTCATTATG TTTGCTTCAA GTGTTCCACT GGCATGTATT 
GCTTGTACCG CAACTGCAGT CATCTATAAA TATGTGGAAT GGAAAGGAGC CAAGAAAGAA 
TGGGGAGATG GAATGAGAGG TTTGGCTCTG ACAACTGCAC AATACAGCCT GCTAAAAGTC 
GAGGACAAAG ATCCACATCC AAAGAATTGG AGACCGCAGG TTTTGATTCT TCTCACAAGT 
CAATGGTCAA AGGAAATGAT TGATAGAAGA GCTGTGTCAA TGCTAAATTT GGGGGCACAG 
TTGAAAGCTG GACGAGGACT TGCTATTGCT TGTGCTTTTT TGAAAGGTTC AGTTGATTCC 
CAGAAAGATA AAAACCGTGC AAGAGACGTT AAAACCACTC TTGTAAAAGA TATGTCAAGT 
GTTCGTCTTC GTGGATTTGC CAAAACCATG TTCTATAACA ATCATCAAAT TAATGGAACT 
ATATCTGGAC TTTATCAGTC AATTGGAATT GGAGGATTGA GACCGAACAC TATTCTACTG 
AATTGGCCGA ATGAGAAGAA TCCCGATGAG CTTGTTCTTT TTGCTGAGGA AATTATTCAT 
GGAGCTGCAA ATGATAACTG TTTGATCGTC ACCAAAGGAA TCACAGATTT CCCTGAATAC 
TCTGAACGTC TAACTGGTTT TATTGATATT TGGTGGATTG TACAAGATGG AGGAATTCTT 
ATGCTTATTG CTTATCTTCT TCGTCAGCAT AAAGTATGGA AAGGTTGCAC ACTTCGAATT 
TTTGCAGTCA GTGAACAAGA TTCTACGAAA TCAGAAGATA TGAAAGCAGG ACTTCAGAAA 
TATATTTATA TGCTGAGAAT TGATGCCGAG CTTTTCATTG TTGATCTTCT TGATATGGAA 
GTGTCAGATG AAGTAGTTGA AAAAGCAGCA GAAGTCGAAA GAAAACAGAA AGAAAGAGAA 
GAAATGAGAA GAAGTAAAAG TGGATATTTG AATGATGGTT TTATGGAAGA CAATGGAAAA 
CCTCGGCAAG TGATGATGCG TCATAGCGAT TCAGCAAGAA GTTTTAGTCC ACAGCCAGGT 
GCACATACTT CCATCAACTT GGATGAGACA GAAACATCAT TTACCGAAAG CCTATTTGAT 
GATTTCTACC GTTCGGGAAC TCCCAATGAA GATCTCGAAG GTGCAATGAA ACTGAACATT 
CACAAAATGA ATACATCCGT TCGTCTAAAT CGTGTTATTC GAGAAAATTC TCCAGACTCT 
CAGTTGATCT TATTGAATCT ACCATCTCCT CCAAGAAACC GTCTTGCTTT CAACAATTCT 
TATATGACAT ATTTGGACGT GTTGACTGAA GATTTGCCAC GTGTACTTTT CATTGGTGGA 
AGTGGCCGTG AAGTTATTAC AATCGACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338797.1
CDS	19..3231
Translation	MSNARRRFSTVTQINTEGLQAMGKGGGRMETVGEDGIPADYKGNRKFTTSLGHLALYKEDEGIGTQASFISGYTTPGPKERATSEHVKANLGVMLGVYLPTIQHILGVTMFIRLFWVVGMSGVAWTMALLAICCLSTLLTSISLSAVATNGVVESGGAYFIISRNLGAEFGSAVGILFYLANTVAASMYIVGGVEVILMYLWPEMAIGGADALHDTEMFGSLYNNLRLYGTVFLLIQALIVAMGVKFVQLLAPVSLMCVILAIAACIGGGIEKQITMEGMKVCAIDNHLLQSSIVTHPIHKNQTSWFNETVDFCNLCDKSLYLESVFCANVNNDEASAEDDVFCTHYTSKKMTCQLAFPGFNMKTLNDNMWPEYMEKSEVVPGVRGKETAEVVQDESSTFFMLMAIYFPAVTGIFTGTNMSGDLRDPQRSIPVGTIAATLTTSAIYYILAILFGGSITRSVLRDKFGRSIGNTMVVAALSWPHPAVVTVGAFLSTFGAALQCLCSAPRLLQSIAKDDVIPILAPFARVTKNNEPFLGLVLTVIIAECGILLGAVDKIAEVLDFFFLMCYAFVNLIAVLHSVLKSPNWRPRFKYFHWTLSLLGAALCFFIMFASSVPLACIACTATAVIYKYVEWKGAKKEWGDGMRGLALTTAQYSLLKVEDKDPHPKNWRPQVLILLTSQWSKEMIDRRAVSMLNLGAQLKAGRGLAIACAFLKGSVDSQKDKNRARDVKTTLVKDMSSVRLRGFAKTMFYNNHQINGTISGLYQSIGIGGLRPNTILLNWPNEKNPDELVLFAEEIIHGAANDNCLIVTKGITDFPEYSERLTGFIDIWWIVQDGGILMLIAYLLRQHKVWKGCTLRIFAVSEQDSTKSEDMKAGLQKYIYMLRIDAELFIVDLLDMEVSDEVVEKAAEVERKQKEREEMRRSKSGYLNDGFMEDNGKPRQVMMRHSDSARSFSPQPGAHTSINLDETETSFTESLFDDFYRSGTPNEDLEGAMKLNIHKMNTSVRLNRVIRENSPDSQLILLNLPSPPRNRLAFNNSYMTYLDVLTEDLPRVLFIGGSGREVITIDS

Target ORF information:

RefSeq Version	NM_001351920.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Potassium/chloride cotransporter 3 (kcc-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351920.2

ORF Insert Sequence:

ATGTCGAATG CAAGGCGCCG TTTCAGTACG GTGACGCAGA TAAATACAGA AGGCCTTCAA 
GCTATGGGAA AAGGCGGGGG TCGAATGGAA ACTGTCGGAG AGGATGGTAT CCCTGCGGAT 
TATAAAGGAA ATCGAAAATT CACGACAAGT CTCGGTCACC TGGCATTATA CAAGGAGGAT 
GAGGGGATTG GGACACAAGC TTCTTTTATT TCGGGTTATA CAACGCCCGG TCCGAAAGAA 
AGAGCAACTT CTGAGCATGT GAAGGCCAAC TTGGGAGTGA TGCTTGGAGT TTATTTACCC 
ACAATTCAAC ATATTCTCGG AGTTACCATG TTTATCCGTC TTTTTTGGGT CGTTGGAATG 
TCTGGCGTTG CTTGGACAAT GGCCCTACTC GCCATTTGTT GTTTATCGAC TCTTTTGACC 
TCAATCTCAT TATCAGCAGT TGCCACAAAC GGAGTCGTTG AATCTGGAGG AGCATATTTC 
ATTATTAGCA GAAATCTTGG AGCTGAATTT GGATCAGCTG TTGGTATTTT GTTTTACTTG 
GCAAACACAG TGGCTGCATC GATGTACATT GTTGGCGGTG TTGAAGTTAT TCTCATGTAT 
TTGTGGCCTG AAATGGCAAT TGGTGGAGCT GATGCACTTC ATGATACTGA AATGTTTGGA 
AGTCTTTATA ATAATCTTCG TCTCTACGGA ACCGTTTTTC TTCTCATTCA AGCTCTCATT 
GTGGCAATGG GTGTCAAATT TGTGCAACTA CTTGCTCCAG TGTCTCTGAT GTGTGTTATA 
TTGGCAATTG CTGCTTGTAT CGGAGGTGGA ATTGAAAAAC AGATTACAAT GGAAGGAATG 
AAAGTATGTG CCATTGACAA TCATTTGTTG CAATCTTCAA TTGTCACTCA CCCAATTCAT 
AAAAATCAGA CTAGTTGGTT TAATGAAACT GTTGATTTCT GCAATCTATG TGATAAATCT 
CTTTATCTTG AAAGTGTTTT CTGTGCAAAT GTGAATAATG ACGAAGCTTC AGCAGAAGAT 
GATGTTTTTT GTACTCACTA TACTTCAAAA AAGATGACAT GCCAATTAGC ATTCCCTGGT 
TTCAATATGA AAACTCTAAA CGACAATATG TGGCCCGAGT ACATGGAAAA ATCAGAAGTG 
GTACCTGGTG TGAGAGGAAA AGAAACTGCT GAAGTTGTAC AAGATGAATC TTCAACATTC 
TTCATGCTTA TGGCTATTTA TTTCCCAGCT GTGACTGGAA TCTTCACTGG AACAAATATG 
AGCGGAGATT TGAGAGATCC ACAAAGAAGT ATCCCTGTTG GAACAATTGC TGCAACACTT 
ACAACTTCAG CAATCTACTA CATACTTGCA ATTTTATTTG GTGGATCGAT AACAAGAAGT 
GTGTTACGTG ACAAATTCGG TCGATCAATT GGAAATACAA TGGTTGTTGC TGCCCTCTCA 
TGGCCACATC CTGCTGTTGT AACTGTTGGA GCATTCTTGT CTACTTTTGG TGCTGCTCTT 
CAATGCCTCT GCAGTGCGCC CCGTCTATTA CAATCGATCG CCAAGGATGA CGTAATCCCG 
ATTCTCGCCC CATTCGCCCG TGTTACAAAA AACAATGAAC CTTTTCTTGG ATTGGTTCTC 
ACTGTCATCA TCGCAGAATG TGGAATCCTT CTTGGAGCTG TGGATAAGAT TGCCGAAGTT 
CTTGATTTCT TTTTCCTTAT GTGTTATGCA TTTGTGAATT TGATAGCAGT TCTTCATTCC 
GTCTTGAAGT CACCAAATTG GCGACCCAGA TTTAAATATT TTCACTGGAC TCTATCACTT 
CTTGGAGCGG CTCTATGCTT CTTCATTATG TTTGCTTCAA GTGTTCCACT GGCATGTATT 
GCTTGTACCG CAACTGCAGT CATCTATAAA TATGTGGAAT GGAAAGGAGC CAAGAAAGAA 
TGGGGAGATG GAATGAGAGG TTTGGCTCTG ACAACTGCAC AATACAGCCT GCTAAAAGTC 
GAGGACAAAG ATCCACATCC AAAGAATTGG AGACCGCAGG TTTTGATTCT TCTCACAAGT 
CAATGGTCAA AGGAAATGAT TGATAGAAGA GCTGTGTCAA TGCTAAATTT GGGGGCACAG 
TTGAAAGCTG GACGAGGACT TGCTATTGCT TGTGCTTTTT TGAAAGGTTC AGTTGATTCC 
CAGAAAGATA AAAACCGTGC AAGAGACGTT AAAACCACTC TTGTAAAAGA TATGTCAAGT 
GTTCGTCTTC GTGGATTTGC CAAAACCATG TTCTATAACA ATCATCAAAT TAATGGAACT 
ATATCTGGAC TTTATCAGTC AATTGGAATT GGAGGATTGA GACCGAACAC TATTCTACTG 
AATTGGCCGA ATGAGAAGAA TCCCGATGAG CTTGTTCTTT TTGCTGAGGA AATTATTCAT 
GGAGCTGCAA ATGATAACTG TTTGATCGTC ACCAAAGGAA TCACAGATTT CCCTGAATAC 
TCTGAACGTC TAACTGGTTT TATTGATATT TGGTGGATTG TACAAGATGG AGGAATTCTT 
ATGCTTATTG CTTATCTTCT TCGTCAGCAT AAAGTATGGA AAGGTTGCAC ACTTCGAATT 
TTTGCAGTCA GTGAACAAGA TTCTACGAAA TCAGAAGATA TGAAAGCAGG ACTTCAGAAA 
TATATTTATA TGCTGAGAAT TGATGCCGAG CTTTTCATTG TTGATCTTCT TGATATGGAA 
GTGTCAGATG AAGTAGTTGA AAAAGCAGCA GAAGTCGAAA GAAAACAGAA AGAAAGAGAA 
GAAATGAGAA GAAGTAAAAG TGGATATTTG AATGATGGTT TTATGGAAGA CAATGGAAAA 
CCTCGGCAAG TGATGATGCG TCATAGCGAT TCAGCAAGAA GTTTTAGTCC ACAGCCAGGT 
GCACATACTT CCATCAACTT GGATGAGACA GAAACATCAT TTACCGAAAG CCTATTTGAT 
GATTTCTACC GTTCGGGAAC TCCCAATGAA GATCTCGAAG GTGCAATGAA ACTGAACATT 
CACAAAATGA ATACATCCGT TCGTCTAAAT CGTGTTATTC GAGAAAATTC TCCAGACTCT 
CAGTTGATCT TATTGAATCT ACCATCTCCT CCAAGAAACC GTCTTGCTTT CAACAATTCT 
TATATGACAT ATTTGGACGT GTTGACTGAA GATTTGCCAC GTGTACTTTT CATTGGTGGA 
AGTGGCCGTG AAGTTATTAC AATCGACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kcc-3 ( NM_001351920.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001338797.1 Caenorhabditis elegans Potassium/chloride cotransporter 3 (kcc-3), partial mRNA.

Price & Availability↑

CloneID	OCa529777
Clone ID Related Accession (Same CDS sequence)	NM_001351920.2 , NM_001351920.1
Accession Version	NM_001351920.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Potassium/chloride cotransporter 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGTCGAATG CAAGGCGCCG TTTCAGTACG GTGACGCAGA TAAATACAGA AGGCCTTCAA 
GCTATGGGAA AAGGCGGGGG TCGAATGGAA ACTGTCGGAG AGGATGGTAT CCCTGCGGAT 
TATAAAGGAA ATCGAAAATT CACGACAAGT CTCGGTCACC TGGCATTATA CAAGGAGGAT 
GAGGGGATTG GGACACAAGC TTCTTTTATT TCGGGTTATA CAACGCCCGG TCCGAAAGAA 
AGAGCAACTT CTGAGCATGT GAAGGCCAAC TTGGGAGTGA TGCTTGGAGT TTATTTACCC 
ACAATTCAAC ATATTCTCGG AGTTACCATG TTTATCCGTC TTTTTTGGGT CGTTGGAATG 
TCTGGCGTTG CTTGGACAAT GGCCCTACTC GCCATTTGTT GTTTATCGAC TCTTTTGACC 
TCAATCTCAT TATCAGCAGT TGCCACAAAC GGAGTCGTTG AATCTGGAGG AGCATATTTC 
ATTATTAGCA GAAATCTTGG AGCTGAATTT GGATCAGCTG TTGGTATTTT GTTTTACTTG 
GCAAACACAG TGGCTGCATC GATGTACATT GTTGGCGGTG TTGAAGTTAT TCTCATGTAT 
TTGTGGCCTG AAATGGCAAT TGGTGGAGCT GATGCACTTC ATGATACTGA AATGTTTGGA 
AGTCTTTATA ATAATCTTCG TCTCTACGGA ACCGTTTTTC TTCTCATTCA AGCTCTCATT 
GTGGCAATGG GTGTCAAATT TGTGCAACTA CTTGCTCCAG TGTCTCTGAT GTGTGTTATA 
TTGGCAATTG CTGCTTGTAT CGGAGGTGGA ATTGAAAAAC AGATTACAAT GGAAGGAATG 
AAAGTATGTG CCATTGACAA TCATTTGTTG CAATCTTCAA TTGTCACTCA CCCAATTCAT 
AAAAATCAGA CTAGTTGGTT TAATGAAACT GTTGATTTCT GCAATCTATG TGATAAATCT 
CTTTATCTTG AAAGTGTTTT CTGTGCAAAT GTGAATAATG ACGAAGCTTC AGCAGAAGAT 
GATGTTTTTT GTACTCACTA TACTTCAAAA AAGATGACAT GCCAATTAGC ATTCCCTGGT 
TTCAATATGA AAACTCTAAA CGACAATATG TGGCCCGAGT ACATGGAAAA ATCAGAAGTG 
GTACCTGGTG TGAGAGGAAA AGAAACTGCT GAAGTTGTAC AAGATGAATC TTCAACATTC 
TTCATGCTTA TGGCTATTTA TTTCCCAGCT GTGACTGGAA TCTTCACTGG AACAAATATG 
AGCGGAGATT TGAGAGATCC ACAAAGAAGT ATCCCTGTTG GAACAATTGC TGCAACACTT 
ACAACTTCAG CAATCTACTA CATACTTGCA ATTTTATTTG GTGGATCGAT AACAAGAAGT 
GTGTTACGTG ACAAATTCGG TCGATCAATT GGAAATACAA TGGTTGTTGC TGCCCTCTCA 
TGGCCACATC CTGCTGTTGT AACTGTTGGA GCATTCTTGT CTACTTTTGG TGCTGCTCTT 
CAATGCCTCT GCAGTGCGCC CCGTCTATTA CAATCGATCG CCAAGGATGA CGTAATCCCG 
ATTCTCGCCC CATTCGCCCG TGTTACAAAA AACAATGAAC CTTTTCTTGG ATTGGTTCTC 
ACTGTCATCA TCGCAGAATG TGGAATCCTT CTTGGAGCTG TGGATAAGAT TGCCGAAGTT 
CTTGATTTCT TTTTCCTTAT GTGTTATGCA TTTGTGAATT TGATAGCAGT TCTTCATTCC 
GTCTTGAAGT CACCAAATTG GCGACCCAGA TTTAAATATT TTCACTGGAC TCTATCACTT 
CTTGGAGCGG CTCTATGCTT CTTCATTATG TTTGCTTCAA GTGTTCCACT GGCATGTATT 
GCTTGTACCG CAACTGCAGT CATCTATAAA TATGTGGAAT GGAAAGGAGC CAAGAAAGAA 
TGGGGAGATG GAATGAGAGG TTTGGCTCTG ACAACTGCAC AATACAGCCT GCTAAAAGTC 
GAGGACAAAG ATCCACATCC AAAGAATTGG AGACCGCAGG TTTTGATTCT TCTCACAAGT 
CAATGGTCAA AGGAAATGAT TGATAGAAGA GCTGTGTCAA TGCTAAATTT GGGGGCACAG 
TTGAAAGCTG GACGAGGACT TGCTATTGCT TGTGCTTTTT TGAAAGGTTC AGTTGATTCC 
CAGAAAGATA AAAACCGTGC AAGAGACGTT AAAACCACTC TTGTAAAAGA TATGTCAAGT 
GTTCGTCTTC GTGGATTTGC CAAAACCATG TTCTATAACA ATCATCAAAT TAATGGAACT 
ATATCTGGAC TTTATCAGTC AATTGGAATT GGAGGATTGA GACCGAACAC TATTCTACTG 
AATTGGCCGA ATGAGAAGAA TCCCGATGAG CTTGTTCTTT TTGCTGAGGA AATTATTCAT 
GGAGCTGCAA ATGATAACTG TTTGATCGTC ACCAAAGGAA TCACAGATTT CCCTGAATAC 
TCTGAACGTC TAACTGGTTT TATTGATATT TGGTGGATTG TACAAGATGG AGGAATTCTT 
ATGCTTATTG CTTATCTTCT TCGTCAGCAT AAAGTATGGA AAGGTTGCAC ACTTCGAATT 
TTTGCAGTCA GTGAACAAGA TTCTACGAAA TCAGAAGATA TGAAAGCAGG ACTTCAGAAA 
TATATTTATA TGCTGAGAAT TGATGCCGAG CTTTTCATTG TTGATCTTCT TGATATGGAA 
GTGTCAGATG AAGTAGTTGA AAAAGCAGCA GAAGTCGAAA GAAAACAGAA AGAAAGAGAA 
GAAATGAGAA GAAGTAAAAG TGGATATTTG AATGATGGTT TTATGGAAGA CAATGGAAAA 
CCTCGGCAAG TGATGATGCG TCATAGCGAT TCAGCAAGAA GTTTTAGTCC ACAGCCAGGT 
GCACATACTT CCATCAACTT GGATGAGACA GAAACATCAT TTACCGAAAG CCTATTTGAT 
GATTTCTACC GTTCGGGAAC TCCCAATGAA GATCTCGAAG GTGCAATGAA ACTGAACATT 
CACAAAATGA ATACATCCGT TCGTCTAAAT CGTGTTATTC GAGAAAATTC TCCAGACTCT 
CAGTTGATCT TATTGAATCT ACCATCTCCT CCAAGAAACC GTCTTGCTTT CAACAATTCT 
TATATGACAT ATTTGGACGT GTTGACTGAA GATTTGCCAC GTGTACTTTT CATTGGTGGA 
AGTGGCCGTG AAGTTATTAC AATCGACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338797.1
CDS	1..3213
Translation	MSNARRRFSTVTQINTEGLQAMGKGGGRMETVGEDGIPADYKGNRKFTTSLGHLALYKEDEGIGTQASFISGYTTPGPKERATSEHVKANLGVMLGVYLPTIQHILGVTMFIRLFWVVGMSGVAWTMALLAICCLSTLLTSISLSAVATNGVVESGGAYFIISRNLGAEFGSAVGILFYLANTVAASMYIVGGVEVILMYLWPEMAIGGADALHDTEMFGSLYNNLRLYGTVFLLIQALIVAMGVKFVQLLAPVSLMCVILAIAACIGGGIEKQITMEGMKVCAIDNHLLQSSIVTHPIHKNQTSWFNETVDFCNLCDKSLYLESVFCANVNNDEASAEDDVFCTHYTSKKMTCQLAFPGFNMKTLNDNMWPEYMEKSEVVPGVRGKETAEVVQDESSTFFMLMAIYFPAVTGIFTGTNMSGDLRDPQRSIPVGTIAATLTTSAIYYILAILFGGSITRSVLRDKFGRSIGNTMVVAALSWPHPAVVTVGAFLSTFGAALQCLCSAPRLLQSIAKDDVIPILAPFARVTKNNEPFLGLVLTVIIAECGILLGAVDKIAEVLDFFFLMCYAFVNLIAVLHSVLKSPNWRPRFKYFHWTLSLLGAALCFFIMFASSVPLACIACTATAVIYKYVEWKGAKKEWGDGMRGLALTTAQYSLLKVEDKDPHPKNWRPQVLILLTSQWSKEMIDRRAVSMLNLGAQLKAGRGLAIACAFLKGSVDSQKDKNRARDVKTTLVKDMSSVRLRGFAKTMFYNNHQINGTISGLYQSIGIGGLRPNTILLNWPNEKNPDELVLFAEEIIHGAANDNCLIVTKGITDFPEYSERLTGFIDIWWIVQDGGILMLIAYLLRQHKVWKGCTLRIFAVSEQDSTKSEDMKAGLQKYIYMLRIDAELFIVDLLDMEVSDEVVEKAAEVERKQKEREEMRRSKSGYLNDGFMEDNGKPRQVMMRHSDSARSFSPQPGAHTSINLDETETSFTESLFDDFYRSGTPNEDLEGAMKLNIHKMNTSVRLNRVIRENSPDSQLILLNLPSPPRNRLAFNNSYMTYLDVLTEDLPRVLFIGGSGREVITIDS

Target ORF information:

RefSeq Version	NM_001351920.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Potassium/chloride cotransporter 3 (kcc-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351920.1

ORF Insert Sequence:

ATGTCGAATG CAAGGCGCCG TTTCAGTACG GTGACGCAGA TAAATACAGA AGGCCTTCAA 
GCTATGGGAA AAGGCGGGGG TCGAATGGAA ACTGTCGGAG AGGATGGTAT CCCTGCGGAT 
TATAAAGGAA ATCGAAAATT CACGACAAGT CTCGGTCACC TGGCATTATA CAAGGAGGAT 
GAGGGGATTG GGACACAAGC TTCTTTTATT TCGGGTTATA CAACGCCCGG TCCGAAAGAA 
AGAGCAACTT CTGAGCATGT GAAGGCCAAC TTGGGAGTGA TGCTTGGAGT TTATTTACCC 
ACAATTCAAC ATATTCTCGG AGTTACCATG TTTATCCGTC TTTTTTGGGT CGTTGGAATG 
TCTGGCGTTG CTTGGACAAT GGCCCTACTC GCCATTTGTT GTTTATCGAC TCTTTTGACC 
TCAATCTCAT TATCAGCAGT TGCCACAAAC GGAGTCGTTG AATCTGGAGG AGCATATTTC 
ATTATTAGCA GAAATCTTGG AGCTGAATTT GGATCAGCTG TTGGTATTTT GTTTTACTTG 
GCAAACACAG TGGCTGCATC GATGTACATT GTTGGCGGTG TTGAAGTTAT TCTCATGTAT 
TTGTGGCCTG AAATGGCAAT TGGTGGAGCT GATGCACTTC ATGATACTGA AATGTTTGGA 
AGTCTTTATA ATAATCTTCG TCTCTACGGA ACCGTTTTTC TTCTCATTCA AGCTCTCATT 
GTGGCAATGG GTGTCAAATT TGTGCAACTA CTTGCTCCAG TGTCTCTGAT GTGTGTTATA 
TTGGCAATTG CTGCTTGTAT CGGAGGTGGA ATTGAAAAAC AGATTACAAT GGAAGGAATG 
AAAGTATGTG CCATTGACAA TCATTTGTTG CAATCTTCAA TTGTCACTCA CCCAATTCAT 
AAAAATCAGA CTAGTTGGTT TAATGAAACT GTTGATTTCT GCAATCTATG TGATAAATCT 
CTTTATCTTG AAAGTGTTTT CTGTGCAAAT GTGAATAATG ACGAAGCTTC AGCAGAAGAT 
GATGTTTTTT GTACTCACTA TACTTCAAAA AAGATGACAT GCCAATTAGC ATTCCCTGGT 
TTCAATATGA AAACTCTAAA CGACAATATG TGGCCCGAGT ACATGGAAAA ATCAGAAGTG 
GTACCTGGTG TGAGAGGAAA AGAAACTGCT GAAGTTGTAC AAGATGAATC TTCAACATTC 
TTCATGCTTA TGGCTATTTA TTTCCCAGCT GTGACTGGAA TCTTCACTGG AACAAATATG 
AGCGGAGATT TGAGAGATCC ACAAAGAAGT ATCCCTGTTG GAACAATTGC TGCAACACTT 
ACAACTTCAG CAATCTACTA CATACTTGCA ATTTTATTTG GTGGATCGAT AACAAGAAGT 
GTGTTACGTG ACAAATTCGG TCGATCAATT GGAAATACAA TGGTTGTTGC TGCCCTCTCA 
TGGCCACATC CTGCTGTTGT AACTGTTGGA GCATTCTTGT CTACTTTTGG TGCTGCTCTT 
CAATGCCTCT GCAGTGCGCC CCGTCTATTA CAATCGATCG CCAAGGATGA CGTAATCCCG 
ATTCTCGCCC CATTCGCCCG TGTTACAAAA AACAATGAAC CTTTTCTTGG ATTGGTTCTC 
ACTGTCATCA TCGCAGAATG TGGAATCCTT CTTGGAGCTG TGGATAAGAT TGCCGAAGTT 
CTTGATTTCT TTTTCCTTAT GTGTTATGCA TTTGTGAATT TGATAGCAGT TCTTCATTCC 
GTCTTGAAGT CACCAAATTG GCGACCCAGA TTTAAATATT TTCACTGGAC TCTATCACTT 
CTTGGAGCGG CTCTATGCTT CTTCATTATG TTTGCTTCAA GTGTTCCACT GGCATGTATT 
GCTTGTACCG CAACTGCAGT CATCTATAAA TATGTGGAAT GGAAAGGAGC CAAGAAAGAA 
TGGGGAGATG GAATGAGAGG TTTGGCTCTG ACAACTGCAC AATACAGCCT GCTAAAAGTC 
GAGGACAAAG ATCCACATCC AAAGAATTGG AGACCGCAGG TTTTGATTCT TCTCACAAGT 
CAATGGTCAA AGGAAATGAT TGATAGAAGA GCTGTGTCAA TGCTAAATTT GGGGGCACAG 
TTGAAAGCTG GACGAGGACT TGCTATTGCT TGTGCTTTTT TGAAAGGTTC AGTTGATTCC 
CAGAAAGATA AAAACCGTGC AAGAGACGTT AAAACCACTC TTGTAAAAGA TATGTCAAGT 
GTTCGTCTTC GTGGATTTGC CAAAACCATG TTCTATAACA ATCATCAAAT TAATGGAACT 
ATATCTGGAC TTTATCAGTC AATTGGAATT GGAGGATTGA GACCGAACAC TATTCTACTG 
AATTGGCCGA ATGAGAAGAA TCCCGATGAG CTTGTTCTTT TTGCTGAGGA AATTATTCAT 
GGAGCTGCAA ATGATAACTG TTTGATCGTC ACCAAAGGAA TCACAGATTT CCCTGAATAC 
TCTGAACGTC TAACTGGTTT TATTGATATT TGGTGGATTG TACAAGATGG AGGAATTCTT 
ATGCTTATTG CTTATCTTCT TCGTCAGCAT AAAGTATGGA AAGGTTGCAC ACTTCGAATT 
TTTGCAGTCA GTGAACAAGA TTCTACGAAA TCAGAAGATA TGAAAGCAGG ACTTCAGAAA 
TATATTTATA TGCTGAGAAT TGATGCCGAG CTTTTCATTG TTGATCTTCT TGATATGGAA 
GTGTCAGATG AAGTAGTTGA AAAAGCAGCA GAAGTCGAAA GAAAACAGAA AGAAAGAGAA 
GAAATGAGAA GAAGTAAAAG TGGATATTTG AATGATGGTT TTATGGAAGA CAATGGAAAA 
CCTCGGCAAG TGATGATGCG TCATAGCGAT TCAGCAAGAA GTTTTAGTCC ACAGCCAGGT 
GCACATACTT CCATCAACTT GGATGAGACA GAAACATCAT TTACCGAAAG CCTATTTGAT 
GATTTCTACC GTTCGGGAAC TCCCAATGAA GATCTCGAAG GTGCAATGAA ACTGAACATT 
CACAAAATGA ATACATCCGT TCGTCTAAAT CGTGTTATTC GAGAAAATTC TCCAGACTCT 
CAGTTGATCT TATTGAATCT ACCATCTCCT CCAAGAAACC GTCTTGCTTT CAACAATTCT 
TATATGACAT ATTTGGACGT GTTGACTGAA GATTTGCCAC GTGTACTTTT CATTGGTGGA 
AGTGGCCGTG AAGTTATTAC AATCGACTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kcc-3 ( NM_001351919.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001338828.1 Caenorhabditis elegans Potassium/chloride cotransporter 3 (kcc-3), partial mRNA.

Price & Availability↑

CloneID	OCa529778
Clone ID Related Accession (Same CDS sequence)	NM_001351919.2 , NM_001351919.1
Accession Version	NM_001351919.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Potassium/chloride cotransporter 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001351919.1. COMPLETENESS: incomplete on the 5' end.

ATGGGAGGTT TCCAGGATCT ACGGCGAACA GTCATGTCGA ATGCAAGGCG CCGTTTCAGT 
ACGGTGACGC AGATAAATAC AGAAGGCCTT CAAGCTATGG GAAAAGGCGG GGGTCGAATG 
GAAACTGTCG GAGAGGATGG TATCCCTGCG GATTATAAAG GAAATCGAAA ATTCACGACA 
AGTCTCGGTC ACCTGGCATT ATACAAGGAG GATGAGGGGA TTGGGACACA AGCTTCTTTT 
ATTTCGGGTT ATACAACGCC CGGTCCGAAA GAAAGAGCAA CTTCTGAGCA TGTGAAGGCC 
AACTTGGGAG TGATGCTTGG AGTTTATTTA CCCACAATTC AACATATTCT CGGAGTTACC 
ATGTTTATCC GTCTTTTTTG GGTCGTTGGA ATGTCTGGCG TTGCTTGGAC AATGGCCCTA 
CTCGCCATTT GTTGTTTATC GACTCTTTTG ACCTCAATCT CATTATCAGC AGTTGCCACA 
AACGGAGTCG TTGAATCTGG AGGAGCATAT TTCATTATTA GCAGAAATCT TGGAGCTGAA 
TTTGGATCAG CTGTTGGTAT TTTGTTTTAC TTGGCAAACA CAGTGGCTGC ATCGATGTAC 
ATTGTTGGCG GTGTTGAAGT TATTCTCATG TATTTGTGGC CTGAAATGGC AATTGGTGGA 
GCTGATGCAC TTCATGATAC TGAAATGTTT GGAAGTCTTT ATAATAATCT TCGTCTCTAC 
GGAACCGTTT TTCTTCTCAT TCAAGCTCTC ATTGTGGCAA TGGGTGTCAA ATTTGTGCAA 
CTACTTGCTC CAGTGTCTCT GATGTGTGTT ATATTGGCAA TTGCTGCTTG TATCGGAGGT 
GGAATTGAAA AACAGATTAC AATGGAAGGA ATGAAAGTAT GTGCCATTGA CAATCATTTG 
TTGCAATCTT CAATTGTCAC TCACCCAATT CATAAAAATC AGACTAGTTG GTTTAATGAA 
ACTGTTGATT TCTGCAATCT ATGTGATAAA TCTCTTTATC TTGAAAGTGT TTTCTGTGCA 
AATGTGAATA ATGACGAAGC TTCAGCAGAA GATGATGTTT TTTGTACTCA CTATACTTCA 
AAAAAGATGA CATGCCAATT AGCATTCCCT GGTTTCAATA TGAAAACTCT AAACGACAAT 
ATGTGGCCCG AGTACATGGA AAAATCAGAA GTGGTACCTG GTGTGAGAGG AAAAGAAACT 
GCTGAAGTTG TACAAGATGA ATCTTCAACA TTCTTCATGC TTATGGCTAT TTATTTCCCA 
GCTGTGACTG GAATCTTCAC TGGAACAAAT ATGAGCGGAG ATTTGAGAGA TCCACAAAGA 
AGTATCCCTG TTGGAACAAT TGCTGCAACA CTTACAACTT CAGCAATCTA CTACATACTT 
GCAATTTTAT TTGGTGGATC GATAACAAGA AGTGTGTTAC GTGACAAATT CGGTCGATCA 
ATTGGAAATA CAATGGTTGT TGCTGCCCTC TCATGGCCAC ATCCTGCTGT TGTAACTGTT 
GGAGCATTCT TGTCTACTTT TGGTGCTGCT CTTCAATGCC TCTGCAGTGC GCCCCGTCTA 
TTACAATCGA TCGCCAAGGA TGACGTAATC CCGATTCTCG CCCCATTCGC CCGTGTTACA 
AAAAACAATG AACCTTTTCT TGGATTGGTT CTCACTGTCA TCATCGCAGA ATGTGGAATC 
CTTCTTGGAG CTGTGGATAA GATTGCCGAA GTTCTTGATT TCTTTTTCCT TATGTGTTAT 
GCATTTGTGA ATTTGATAGC AGTTCTTCAT TCCGTCTTGA AGTCACCAAA TTGGCGACCC 
AGATTTAAAT ATTTTCACTG GACTCTATCA CTTCTTGGAG CGGCTCTATG CTTCTTCATT 
ATGTTTGCTT CAAGTGTTCC ACTGGCATGT ATTGCTTGTA CCGCAACTGC AGTCATCTAT 
AAATATGTGG AATGGAAAGG AGCCAAGAAA GAATGGGGAG ATGGAATGAG AGGTTTGGCT 
CTGACAACTG CACAATACAG CCTGCTAAAA GTCGAGGACA AAGATCCACA TCCAAAGAAT 
TGGAGACCGC AGGTTTTGAT TCTTCTCACA AGTCAATGGT CAAAGGAAAT GATTGATAGA 
AGAGCTGTGT CAATGCTAAA TTTGGGGGCA CAGTTGAAAG CTGGACGAGG ACTTGCTATT 
GCTTGTGCTT TTTTGAAAGG TTCAGTTGAT TCCCAGAAAG ATAAAAACCG TGCAAGAGAC 
GTTAAAACCA CTCTTGTAAA AGATATGTCA AGTGTTCGTC TTCGTGGATT TGCCAAAACC 
ATGTTCTATA ACAATCATCA AATTAATGGA ACTATATCTG GACTTTATCA GTCAATTGGA 
ATTGGAGGAT TGAGACCGAA CACTATTCTA CTGAATTGGC CGAATGAGAA GAATCCCGAT 
GAGCTTGTTC TTTTTGCTGA GGAAATTATT CATGGAGCTG CAAATGATAA CTGTTTGATC 
GTCACCAAAG GAATCACAGA TTTCCCTGAA TACTCTGAAC GTCTAACTGG TTTTATTGAT 
ATTTGGTGGA TTGTACAAGA TGGAGGAATT CTTATGCTTA TTGCTTATCT TCTTCGTCAG 
CATAAAGTAT GGAAAGGTTG CACACTTCGA ATTTTTGCAG TCAGTGAACA AGATTCTACG 
AAATCAGAAG ATATGAAAGC AGGACTTCAG AAATATATTT ATATGCTGAG AATTGATGCC 
GAGCTTTTCA TTGTTGATCT TCTTGATATG GAAGTGTCAG ATGAAGTAGT TGAAAAAGCA 
GCAGAAGTCG AAAGAAAACA GAAAGAAAGA GAAGAAATGA GAAGAAGTAA AAGTGGATAT 
TTGAATGATG GTTTTATGGA AGACAATGGA AAACCTCGGC AAGTGATGAT GCGTCATAGC 
GATTCAGCAA GAAGTTTTAG TCCACAGCCA GGTGCACATA CTTCCATCAA CTTGGATGAG 
ACAGAAACAT CATTTACCGA AAGCCTATTT GATGATTTCT ACCGTTCGGG AACTCCCAAT 
GAAGATCTCG AAGGTGCAAT GAAACTGAAC ATTCACAAAA TGAATACATC CGTTCGTCTA 
AATCGTGTTA TTCGAGAAAA TTCTCCAGAC TCTCAGTTGA TCTTATTGAA TCTACCATCT 
CCTCCAAGAA ACCGTCTTGC TTTCAACAAT TCTTATATGA CATATTTGGA CGTGTTGACT 
GAAGATTTGC CACGTGTACT TTTCATTGGT GGAAGTGGCC GTGAAGTTAT TACAATCGAC 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338828.1
CDS	1..3246
Translation	MGGFQDLRRTVMSNARRRFSTVTQINTEGLQAMGKGGGRMETVGEDGIPADYKGNRKFTTSLGHLALYKEDEGIGTQASFISGYTTPGPKERATSEHVKANLGVMLGVYLPTIQHILGVTMFIRLFWVVGMSGVAWTMALLAICCLSTLLTSISLSAVATNGVVESGGAYFIISRNLGAEFGSAVGILFYLANTVAASMYIVGGVEVILMYLWPEMAIGGADALHDTEMFGSLYNNLRLYGTVFLLIQALIVAMGVKFVQLLAPVSLMCVILAIAACIGGGIEKQITMEGMKVCAIDNHLLQSSIVTHPIHKNQTSWFNETVDFCNLCDKSLYLESVFCANVNNDEASAEDDVFCTHYTSKKMTCQLAFPGFNMKTLNDNMWPEYMEKSEVVPGVRGKETAEVVQDESSTFFMLMAIYFPAVTGIFTGTNMSGDLRDPQRSIPVGTIAATLTTSAIYYILAILFGGSITRSVLRDKFGRSIGNTMVVAALSWPHPAVVTVGAFLSTFGAALQCLCSAPRLLQSIAKDDVIPILAPFARVTKNNEPFLGLVLTVIIAECGILLGAVDKIAEVLDFFFLMCYAFVNLIAVLHSVLKSPNWRPRFKYFHWTLSLLGAALCFFIMFASSVPLACIACTATAVIYKYVEWKGAKKEWGDGMRGLALTTAQYSLLKVEDKDPHPKNWRPQVLILLTSQWSKEMIDRRAVSMLNLGAQLKAGRGLAIACAFLKGSVDSQKDKNRARDVKTTLVKDMSSVRLRGFAKTMFYNNHQINGTISGLYQSIGIGGLRPNTILLNWPNEKNPDELVLFAEEIIHGAANDNCLIVTKGITDFPEYSERLTGFIDIWWIVQDGGILMLIAYLLRQHKVWKGCTLRIFAVSEQDSTKSEDMKAGLQKYIYMLRIDAELFIVDLLDMEVSDEVVEKAAEVERKQKEREEMRRSKSGYLNDGFMEDNGKPRQVMMRHSDSARSFSPQPGAHTSINLDETETSFTESLFDDFYRSGTPNEDLEGAMKLNIHKMNTSVRLNRVIRENSPDSQLILLNLPSPPRNRLAFNNSYMTYLDVLTEDLPRVLFIGGSGREVITIDS

Target ORF information:

RefSeq Version	NM_001351919.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Potassium/chloride cotransporter 3 (kcc-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351919.2

ORF Insert Sequence:

ATGGGAGGTT TCCAGGATCT ACGGCGAACA GTCATGTCGA ATGCAAGGCG CCGTTTCAGT 
ACGGTGACGC AGATAAATAC AGAAGGCCTT CAAGCTATGG GAAAAGGCGG GGGTCGAATG 
GAAACTGTCG GAGAGGATGG TATCCCTGCG GATTATAAAG GAAATCGAAA ATTCACGACA 
AGTCTCGGTC ACCTGGCATT ATACAAGGAG GATGAGGGGA TTGGGACACA AGCTTCTTTT 
ATTTCGGGTT ATACAACGCC CGGTCCGAAA GAAAGAGCAA CTTCTGAGCA TGTGAAGGCC 
AACTTGGGAG TGATGCTTGG AGTTTATTTA CCCACAATTC AACATATTCT CGGAGTTACC 
ATGTTTATCC GTCTTTTTTG GGTCGTTGGA ATGTCTGGCG TTGCTTGGAC AATGGCCCTA 
CTCGCCATTT GTTGTTTATC GACTCTTTTG ACCTCAATCT CATTATCAGC AGTTGCCACA 
AACGGAGTCG TTGAATCTGG AGGAGCATAT TTCATTATTA GCAGAAATCT TGGAGCTGAA 
TTTGGATCAG CTGTTGGTAT TTTGTTTTAC TTGGCAAACA CAGTGGCTGC ATCGATGTAC 
ATTGTTGGCG GTGTTGAAGT TATTCTCATG TATTTGTGGC CTGAAATGGC AATTGGTGGA 
GCTGATGCAC TTCATGATAC TGAAATGTTT GGAAGTCTTT ATAATAATCT TCGTCTCTAC 
GGAACCGTTT TTCTTCTCAT TCAAGCTCTC ATTGTGGCAA TGGGTGTCAA ATTTGTGCAA 
CTACTTGCTC CAGTGTCTCT GATGTGTGTT ATATTGGCAA TTGCTGCTTG TATCGGAGGT 
GGAATTGAAA AACAGATTAC AATGGAAGGA ATGAAAGTAT GTGCCATTGA CAATCATTTG 
TTGCAATCTT CAATTGTCAC TCACCCAATT CATAAAAATC AGACTAGTTG GTTTAATGAA 
ACTGTTGATT TCTGCAATCT ATGTGATAAA TCTCTTTATC TTGAAAGTGT TTTCTGTGCA 
AATGTGAATA ATGACGAAGC TTCAGCAGAA GATGATGTTT TTTGTACTCA CTATACTTCA 
AAAAAGATGA CATGCCAATT AGCATTCCCT GGTTTCAATA TGAAAACTCT AAACGACAAT 
ATGTGGCCCG AGTACATGGA AAAATCAGAA GTGGTACCTG GTGTGAGAGG AAAAGAAACT 
GCTGAAGTTG TACAAGATGA ATCTTCAACA TTCTTCATGC TTATGGCTAT TTATTTCCCA 
GCTGTGACTG GAATCTTCAC TGGAACAAAT ATGAGCGGAG ATTTGAGAGA TCCACAAAGA 
AGTATCCCTG TTGGAACAAT TGCTGCAACA CTTACAACTT CAGCAATCTA CTACATACTT 
GCAATTTTAT TTGGTGGATC GATAACAAGA AGTGTGTTAC GTGACAAATT CGGTCGATCA 
ATTGGAAATA CAATGGTTGT TGCTGCCCTC TCATGGCCAC ATCCTGCTGT TGTAACTGTT 
GGAGCATTCT TGTCTACTTT TGGTGCTGCT CTTCAATGCC TCTGCAGTGC GCCCCGTCTA 
TTACAATCGA TCGCCAAGGA TGACGTAATC CCGATTCTCG CCCCATTCGC CCGTGTTACA 
AAAAACAATG AACCTTTTCT TGGATTGGTT CTCACTGTCA TCATCGCAGA ATGTGGAATC 
CTTCTTGGAG CTGTGGATAA GATTGCCGAA GTTCTTGATT TCTTTTTCCT TATGTGTTAT 
GCATTTGTGA ATTTGATAGC AGTTCTTCAT TCCGTCTTGA AGTCACCAAA TTGGCGACCC 
AGATTTAAAT ATTTTCACTG GACTCTATCA CTTCTTGGAG CGGCTCTATG CTTCTTCATT 
ATGTTTGCTT CAAGTGTTCC ACTGGCATGT ATTGCTTGTA CCGCAACTGC AGTCATCTAT 
AAATATGTGG AATGGAAAGG AGCCAAGAAA GAATGGGGAG ATGGAATGAG AGGTTTGGCT 
CTGACAACTG CACAATACAG CCTGCTAAAA GTCGAGGACA AAGATCCACA TCCAAAGAAT 
TGGAGACCGC AGGTTTTGAT TCTTCTCACA AGTCAATGGT CAAAGGAAAT GATTGATAGA 
AGAGCTGTGT CAATGCTAAA TTTGGGGGCA CAGTTGAAAG CTGGACGAGG ACTTGCTATT 
GCTTGTGCTT TTTTGAAAGG TTCAGTTGAT TCCCAGAAAG ATAAAAACCG TGCAAGAGAC 
GTTAAAACCA CTCTTGTAAA AGATATGTCA AGTGTTCGTC TTCGTGGATT TGCCAAAACC 
ATGTTCTATA ACAATCATCA AATTAATGGA ACTATATCTG GACTTTATCA GTCAATTGGA 
ATTGGAGGAT TGAGACCGAA CACTATTCTA CTGAATTGGC CGAATGAGAA GAATCCCGAT 
GAGCTTGTTC TTTTTGCTGA GGAAATTATT CATGGAGCTG CAAATGATAA CTGTTTGATC 
GTCACCAAAG GAATCACAGA TTTCCCTGAA TACTCTGAAC GTCTAACTGG TTTTATTGAT 
ATTTGGTGGA TTGTACAAGA TGGAGGAATT CTTATGCTTA TTGCTTATCT TCTTCGTCAG 
CATAAAGTAT GGAAAGGTTG CACACTTCGA ATTTTTGCAG TCAGTGAACA AGATTCTACG 
AAATCAGAAG ATATGAAAGC AGGACTTCAG AAATATATTT ATATGCTGAG AATTGATGCC 
GAGCTTTTCA TTGTTGATCT TCTTGATATG GAAGTGTCAG ATGAAGTAGT TGAAAAAGCA 
GCAGAAGTCG AAAGAAAACA GAAAGAAAGA GAAGAAATGA GAAGAAGTAA AAGTGGATAT 
TTGAATGATG GTTTTATGGA AGACAATGGA AAACCTCGGC AAGTGATGAT GCGTCATAGC 
GATTCAGCAA GAAGTTTTAG TCCACAGCCA GGTGCACATA CTTCCATCAA CTTGGATGAG 
ACAGAAACAT CATTTACCGA AAGCCTATTT GATGATTTCT ACCGTTCGGG AACTCCCAAT 
GAAGATCTCG AAGGTGCAAT GAAACTGAAC ATTCACAAAA TGAATACATC CGTTCGTCTA 
AATCGTGTTA TTCGAGAAAA TTCTCCAGAC TCTCAGTTGA TCTTATTGAA TCTACCATCT 
CCTCCAAGAA ACCGTCTTGC TTTCAACAAT TCTTATATGA CATATTTGGA CGTGTTGACT 
GAAGATTTGC CACGTGTACT TTTCATTGGT GGAAGTGGCC GTGAAGTTAT TACAATCGAC 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kcc-3 ( NM_001351919.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001338828.1 Caenorhabditis elegans Potassium/chloride cotransporter 3 (kcc-3), partial mRNA.

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CloneID	OCa529778
Clone ID Related Accession (Same CDS sequence)	NM_001351919.2 , NM_001351919.1
Accession Version	NM_001351919.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Potassium/chloride cotransporter 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGGGAGGTT TCCAGGATCT ACGGCGAACA GTCATGTCGA ATGCAAGGCG CCGTTTCAGT 
ACGGTGACGC AGATAAATAC AGAAGGCCTT CAAGCTATGG GAAAAGGCGG GGGTCGAATG 
GAAACTGTCG GAGAGGATGG TATCCCTGCG GATTATAAAG GAAATCGAAA ATTCACGACA 
AGTCTCGGTC ACCTGGCATT ATACAAGGAG GATGAGGGGA TTGGGACACA AGCTTCTTTT 
ATTTCGGGTT ATACAACGCC CGGTCCGAAA GAAAGAGCAA CTTCTGAGCA TGTGAAGGCC 
AACTTGGGAG TGATGCTTGG AGTTTATTTA CCCACAATTC AACATATTCT CGGAGTTACC 
ATGTTTATCC GTCTTTTTTG GGTCGTTGGA ATGTCTGGCG TTGCTTGGAC AATGGCCCTA 
CTCGCCATTT GTTGTTTATC GACTCTTTTG ACCTCAATCT CATTATCAGC AGTTGCCACA 
AACGGAGTCG TTGAATCTGG AGGAGCATAT TTCATTATTA GCAGAAATCT TGGAGCTGAA 
TTTGGATCAG CTGTTGGTAT TTTGTTTTAC TTGGCAAACA CAGTGGCTGC ATCGATGTAC 
ATTGTTGGCG GTGTTGAAGT TATTCTCATG TATTTGTGGC CTGAAATGGC AATTGGTGGA 
GCTGATGCAC TTCATGATAC TGAAATGTTT GGAAGTCTTT ATAATAATCT TCGTCTCTAC 
GGAACCGTTT TTCTTCTCAT TCAAGCTCTC ATTGTGGCAA TGGGTGTCAA ATTTGTGCAA 
CTACTTGCTC CAGTGTCTCT GATGTGTGTT ATATTGGCAA TTGCTGCTTG TATCGGAGGT 
GGAATTGAAA AACAGATTAC AATGGAAGGA ATGAAAGTAT GTGCCATTGA CAATCATTTG 
TTGCAATCTT CAATTGTCAC TCACCCAATT CATAAAAATC AGACTAGTTG GTTTAATGAA 
ACTGTTGATT TCTGCAATCT ATGTGATAAA TCTCTTTATC TTGAAAGTGT TTTCTGTGCA 
AATGTGAATA ATGACGAAGC TTCAGCAGAA GATGATGTTT TTTGTACTCA CTATACTTCA 
AAAAAGATGA CATGCCAATT AGCATTCCCT GGTTTCAATA TGAAAACTCT AAACGACAAT 
ATGTGGCCCG AGTACATGGA AAAATCAGAA GTGGTACCTG GTGTGAGAGG AAAAGAAACT 
GCTGAAGTTG TACAAGATGA ATCTTCAACA TTCTTCATGC TTATGGCTAT TTATTTCCCA 
GCTGTGACTG GAATCTTCAC TGGAACAAAT ATGAGCGGAG ATTTGAGAGA TCCACAAAGA 
AGTATCCCTG TTGGAACAAT TGCTGCAACA CTTACAACTT CAGCAATCTA CTACATACTT 
GCAATTTTAT TTGGTGGATC GATAACAAGA AGTGTGTTAC GTGACAAATT CGGTCGATCA 
ATTGGAAATA CAATGGTTGT TGCTGCCCTC TCATGGCCAC ATCCTGCTGT TGTAACTGTT 
GGAGCATTCT TGTCTACTTT TGGTGCTGCT CTTCAATGCC TCTGCAGTGC GCCCCGTCTA 
TTACAATCGA TCGCCAAGGA TGACGTAATC CCGATTCTCG CCCCATTCGC CCGTGTTACA 
AAAAACAATG AACCTTTTCT TGGATTGGTT CTCACTGTCA TCATCGCAGA ATGTGGAATC 
CTTCTTGGAG CTGTGGATAA GATTGCCGAA GTTCTTGATT TCTTTTTCCT TATGTGTTAT 
GCATTTGTGA ATTTGATAGC AGTTCTTCAT TCCGTCTTGA AGTCACCAAA TTGGCGACCC 
AGATTTAAAT ATTTTCACTG GACTCTATCA CTTCTTGGAG CGGCTCTATG CTTCTTCATT 
ATGTTTGCTT CAAGTGTTCC ACTGGCATGT ATTGCTTGTA CCGCAACTGC AGTCATCTAT 
AAATATGTGG AATGGAAAGG AGCCAAGAAA GAATGGGGAG ATGGAATGAG AGGTTTGGCT 
CTGACAACTG CACAATACAG CCTGCTAAAA GTCGAGGACA AAGATCCACA TCCAAAGAAT 
TGGAGACCGC AGGTTTTGAT TCTTCTCACA AGTCAATGGT CAAAGGAAAT GATTGATAGA 
AGAGCTGTGT CAATGCTAAA TTTGGGGGCA CAGTTGAAAG CTGGACGAGG ACTTGCTATT 
GCTTGTGCTT TTTTGAAAGG TTCAGTTGAT TCCCAGAAAG ATAAAAACCG TGCAAGAGAC 
GTTAAAACCA CTCTTGTAAA AGATATGTCA AGTGTTCGTC TTCGTGGATT TGCCAAAACC 
ATGTTCTATA ACAATCATCA AATTAATGGA ACTATATCTG GACTTTATCA GTCAATTGGA 
ATTGGAGGAT TGAGACCGAA CACTATTCTA CTGAATTGGC CGAATGAGAA GAATCCCGAT 
GAGCTTGTTC TTTTTGCTGA GGAAATTATT CATGGAGCTG CAAATGATAA CTGTTTGATC 
GTCACCAAAG GAATCACAGA TTTCCCTGAA TACTCTGAAC GTCTAACTGG TTTTATTGAT 
ATTTGGTGGA TTGTACAAGA TGGAGGAATT CTTATGCTTA TTGCTTATCT TCTTCGTCAG 
CATAAAGTAT GGAAAGGTTG CACACTTCGA ATTTTTGCAG TCAGTGAACA AGATTCTACG 
AAATCAGAAG ATATGAAAGC AGGACTTCAG AAATATATTT ATATGCTGAG AATTGATGCC 
GAGCTTTTCA TTGTTGATCT TCTTGATATG GAAGTGTCAG ATGAAGTAGT TGAAAAAGCA 
GCAGAAGTCG AAAGAAAACA GAAAGAAAGA GAAGAAATGA GAAGAAGTAA AAGTGGATAT 
TTGAATGATG GTTTTATGGA AGACAATGGA AAACCTCGGC AAGTGATGAT GCGTCATAGC 
GATTCAGCAA GAAGTTTTAG TCCACAGCCA GGTGCACATA CTTCCATCAA CTTGGATGAG 
ACAGAAACAT CATTTACCGA AAGCCTATTT GATGATTTCT ACCGTTCGGG AACTCCCAAT 
GAAGATCTCG AAGGTGCAAT GAAACTGAAC ATTCACAAAA TGAATACATC CGTTCGTCTA 
AATCGTGTTA TTCGAGAAAA TTCTCCAGAC TCTCAGTTGA TCTTATTGAA TCTACCATCT 
CCTCCAAGAA ACCGTCTTGC TTTCAACAAT TCTTATATGA CATATTTGGA CGTGTTGACT 
GAAGATTTGC CACGTGTACT TTTCATTGGT GGAAGTGGCC GTGAAGTTAT TACAATCGAC 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338828.1
CDS	1..3246
Translation	MGGFQDLRRTVMSNARRRFSTVTQINTEGLQAMGKGGGRMETVGEDGIPADYKGNRKFTTSLGHLALYKEDEGIGTQASFISGYTTPGPKERATSEHVKANLGVMLGVYLPTIQHILGVTMFIRLFWVVGMSGVAWTMALLAICCLSTLLTSISLSAVATNGVVESGGAYFIISRNLGAEFGSAVGILFYLANTVAASMYIVGGVEVILMYLWPEMAIGGADALHDTEMFGSLYNNLRLYGTVFLLIQALIVAMGVKFVQLLAPVSLMCVILAIAACIGGGIEKQITMEGMKVCAIDNHLLQSSIVTHPIHKNQTSWFNETVDFCNLCDKSLYLESVFCANVNNDEASAEDDVFCTHYTSKKMTCQLAFPGFNMKTLNDNMWPEYMEKSEVVPGVRGKETAEVVQDESSTFFMLMAIYFPAVTGIFTGTNMSGDLRDPQRSIPVGTIAATLTTSAIYYILAILFGGSITRSVLRDKFGRSIGNTMVVAALSWPHPAVVTVGAFLSTFGAALQCLCSAPRLLQSIAKDDVIPILAPFARVTKNNEPFLGLVLTVIIAECGILLGAVDKIAEVLDFFFLMCYAFVNLIAVLHSVLKSPNWRPRFKYFHWTLSLLGAALCFFIMFASSVPLACIACTATAVIYKYVEWKGAKKEWGDGMRGLALTTAQYSLLKVEDKDPHPKNWRPQVLILLTSQWSKEMIDRRAVSMLNLGAQLKAGRGLAIACAFLKGSVDSQKDKNRARDVKTTLVKDMSSVRLRGFAKTMFYNNHQINGTISGLYQSIGIGGLRPNTILLNWPNEKNPDELVLFAEEIIHGAANDNCLIVTKGITDFPEYSERLTGFIDIWWIVQDGGILMLIAYLLRQHKVWKGCTLRIFAVSEQDSTKSEDMKAGLQKYIYMLRIDAELFIVDLLDMEVSDEVVEKAAEVERKQKEREEMRRSKSGYLNDGFMEDNGKPRQVMMRHSDSARSFSPQPGAHTSINLDETETSFTESLFDDFYRSGTPNEDLEGAMKLNIHKMNTSVRLNRVIRENSPDSQLILLNLPSPPRNRLAFNNSYMTYLDVLTEDLPRVLFIGGSGREVITIDS

Target ORF information:

RefSeq Version	NM_001351919.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Potassium/chloride cotransporter 3 (kcc-3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351919.1

ORF Insert Sequence:

ATGGGAGGTT TCCAGGATCT ACGGCGAACA GTCATGTCGA ATGCAAGGCG CCGTTTCAGT 
ACGGTGACGC AGATAAATAC AGAAGGCCTT CAAGCTATGG GAAAAGGCGG GGGTCGAATG 
GAAACTGTCG GAGAGGATGG TATCCCTGCG GATTATAAAG GAAATCGAAA ATTCACGACA 
AGTCTCGGTC ACCTGGCATT ATACAAGGAG GATGAGGGGA TTGGGACACA AGCTTCTTTT 
ATTTCGGGTT ATACAACGCC CGGTCCGAAA GAAAGAGCAA CTTCTGAGCA TGTGAAGGCC 
AACTTGGGAG TGATGCTTGG AGTTTATTTA CCCACAATTC AACATATTCT CGGAGTTACC 
ATGTTTATCC GTCTTTTTTG GGTCGTTGGA ATGTCTGGCG TTGCTTGGAC AATGGCCCTA 
CTCGCCATTT GTTGTTTATC GACTCTTTTG ACCTCAATCT CATTATCAGC AGTTGCCACA 
AACGGAGTCG TTGAATCTGG AGGAGCATAT TTCATTATTA GCAGAAATCT TGGAGCTGAA 
TTTGGATCAG CTGTTGGTAT TTTGTTTTAC TTGGCAAACA CAGTGGCTGC ATCGATGTAC 
ATTGTTGGCG GTGTTGAAGT TATTCTCATG TATTTGTGGC CTGAAATGGC AATTGGTGGA 
GCTGATGCAC TTCATGATAC TGAAATGTTT GGAAGTCTTT ATAATAATCT TCGTCTCTAC 
GGAACCGTTT TTCTTCTCAT TCAAGCTCTC ATTGTGGCAA TGGGTGTCAA ATTTGTGCAA 
CTACTTGCTC CAGTGTCTCT GATGTGTGTT ATATTGGCAA TTGCTGCTTG TATCGGAGGT 
GGAATTGAAA AACAGATTAC AATGGAAGGA ATGAAAGTAT GTGCCATTGA CAATCATTTG 
TTGCAATCTT CAATTGTCAC TCACCCAATT CATAAAAATC AGACTAGTTG GTTTAATGAA 
ACTGTTGATT TCTGCAATCT ATGTGATAAA TCTCTTTATC TTGAAAGTGT TTTCTGTGCA 
AATGTGAATA ATGACGAAGC TTCAGCAGAA GATGATGTTT TTTGTACTCA CTATACTTCA 
AAAAAGATGA CATGCCAATT AGCATTCCCT GGTTTCAATA TGAAAACTCT AAACGACAAT 
ATGTGGCCCG AGTACATGGA AAAATCAGAA GTGGTACCTG GTGTGAGAGG AAAAGAAACT 
GCTGAAGTTG TACAAGATGA ATCTTCAACA TTCTTCATGC TTATGGCTAT TTATTTCCCA 
GCTGTGACTG GAATCTTCAC TGGAACAAAT ATGAGCGGAG ATTTGAGAGA TCCACAAAGA 
AGTATCCCTG TTGGAACAAT TGCTGCAACA CTTACAACTT CAGCAATCTA CTACATACTT 
GCAATTTTAT TTGGTGGATC GATAACAAGA AGTGTGTTAC GTGACAAATT CGGTCGATCA 
ATTGGAAATA CAATGGTTGT TGCTGCCCTC TCATGGCCAC ATCCTGCTGT TGTAACTGTT 
GGAGCATTCT TGTCTACTTT TGGTGCTGCT CTTCAATGCC TCTGCAGTGC GCCCCGTCTA 
TTACAATCGA TCGCCAAGGA TGACGTAATC CCGATTCTCG CCCCATTCGC CCGTGTTACA 
AAAAACAATG AACCTTTTCT TGGATTGGTT CTCACTGTCA TCATCGCAGA ATGTGGAATC 
CTTCTTGGAG CTGTGGATAA GATTGCCGAA GTTCTTGATT TCTTTTTCCT TATGTGTTAT 
GCATTTGTGA ATTTGATAGC AGTTCTTCAT TCCGTCTTGA AGTCACCAAA TTGGCGACCC 
AGATTTAAAT ATTTTCACTG GACTCTATCA CTTCTTGGAG CGGCTCTATG CTTCTTCATT 
ATGTTTGCTT CAAGTGTTCC ACTGGCATGT ATTGCTTGTA CCGCAACTGC AGTCATCTAT 
AAATATGTGG AATGGAAAGG AGCCAAGAAA GAATGGGGAG ATGGAATGAG AGGTTTGGCT 
CTGACAACTG CACAATACAG CCTGCTAAAA GTCGAGGACA AAGATCCACA TCCAAAGAAT 
TGGAGACCGC AGGTTTTGAT TCTTCTCACA AGTCAATGGT CAAAGGAAAT GATTGATAGA 
AGAGCTGTGT CAATGCTAAA TTTGGGGGCA CAGTTGAAAG CTGGACGAGG ACTTGCTATT 
GCTTGTGCTT TTTTGAAAGG TTCAGTTGAT TCCCAGAAAG ATAAAAACCG TGCAAGAGAC 
GTTAAAACCA CTCTTGTAAA AGATATGTCA AGTGTTCGTC TTCGTGGATT TGCCAAAACC 
ATGTTCTATA ACAATCATCA AATTAATGGA ACTATATCTG GACTTTATCA GTCAATTGGA 
ATTGGAGGAT TGAGACCGAA CACTATTCTA CTGAATTGGC CGAATGAGAA GAATCCCGAT 
GAGCTTGTTC TTTTTGCTGA GGAAATTATT CATGGAGCTG CAAATGATAA CTGTTTGATC 
GTCACCAAAG GAATCACAGA TTTCCCTGAA TACTCTGAAC GTCTAACTGG TTTTATTGAT 
ATTTGGTGGA TTGTACAAGA TGGAGGAATT CTTATGCTTA TTGCTTATCT TCTTCGTCAG 
CATAAAGTAT GGAAAGGTTG CACACTTCGA ATTTTTGCAG TCAGTGAACA AGATTCTACG 
AAATCAGAAG ATATGAAAGC AGGACTTCAG AAATATATTT ATATGCTGAG AATTGATGCC 
GAGCTTTTCA TTGTTGATCT TCTTGATATG GAAGTGTCAG ATGAAGTAGT TGAAAAAGCA 
GCAGAAGTCG AAAGAAAACA GAAAGAAAGA GAAGAAATGA GAAGAAGTAA AAGTGGATAT 
TTGAATGATG GTTTTATGGA AGACAATGGA AAACCTCGGC AAGTGATGAT GCGTCATAGC 
GATTCAGCAA GAAGTTTTAG TCCACAGCCA GGTGCACATA CTTCCATCAA CTTGGATGAG 
ACAGAAACAT CATTTACCGA AAGCCTATTT GATGATTTCT ACCGTTCGGG AACTCCCAAT 
GAAGATCTCG AAGGTGCAAT GAAACTGAAC ATTCACAAAA TGAATACATC CGTTCGTCTA 
AATCGTGTTA TTCGAGAAAA TTCTCCAGAC TCTCAGTTGA TCTTATTGAA TCTACCATCT 
CCTCCAAGAA ACCGTCTTGC TTTCAACAAT TCTTATATGA CATATTTGGA CGTGTTGACT 
GAAGATTTGC CACGTGTACT TTTCATTGGT GGAAGTGGCC GTGAAGTTAT TACAATCGAC 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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kcc-3 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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