alg-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	alg-2
Entrez Gene ID	173468
Full Name	Protein argonaute
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_061436.3	NP_493837.1	Protein argonaute
NM_182192.3	NP_871992.1	Protein argonaute
NM_061436.4	NP_493837.1	Protein argonaute
NM_182192.4	NP_871992.1	Protein argonaute

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ago2	NP_001004877.1
Pan troglodytes (chimpanzee)	EIF2C2	XP_001142838.1
Rattus norvegicus (Norway rat)	Ago2	NP_067608.1
Drosophila melanogaster (fruit fly)	AGO1	NP_001246314.1
Homo sapiens (human)	AGO2	NP_036286.2
Canis lupus familiaris (dog)	AGO2	XP_005628049.1
Bos taurus (cattle)	EIF2C2	NP_991363.1
Danio rerio (zebrafish)	LOC570630	XP_699226.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011717	XP_320795.4
Mus musculus (house mouse)	Ago2	NP_694818.3
Caenorhabditis elegans (roundworm)	alg-1	NP_001257238.1
Arabidopsis thaliana (thale cress)	AGO5	NP_850110.1
	LOC711873	XP_001100725.2
Gallus gallus (chicken)	LOC100858102	XP_004940122.1
Caenorhabditis elegans (roundworm)	alg-2	NP_871992.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

knockdown of alg-1 and alg-2 in germ cells results in few defects in oocyte maturation and ovulation.
Title: Functional analysis of microRNA pathway genes in the somatic gonad and germ cells during ovulation in C. elegans.

The slicing activity of ALG-2 is important to coordinate Dicer cleavage and thus is required for the formation of miRNA-induced silencing complex in C. elegans.
Title: The slicing activity of miRNA-specific Argonautes is essential for the miRNA pathway in C. elegans.

genetic and biochemical data hint at individual nonredundant functions for ALG-1 and ALG-2 in the processing of precursor miRNAs to mature miRNAs
Title: The Caenorhabditis elegans Argonautes ALG-1 and ALG-2: almost identical yet different.

The following alg-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alg-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa339395	NM_182192.4 Latest version!	Caenorhabditis elegans Protein argonaute (alg-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa339396	NM_061436.4 Latest version!	Caenorhabditis elegans Protein argonaute (alg-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa339396	NM_061436.3	Caenorhabditis elegans Protein argonaute (alg-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa339395	NM_182192.3	Caenorhabditis elegans Protein argonaute (alg-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alg-2 ( NM_182192.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_871992.1 Caenorhabditis elegans Protein argonaute (alg-2), partial mRNA.

Price & Availability↑

CloneID	OCa339395
Clone ID Related Accession (Same CDS sequence)	NM_182192.3 , NM_182192.4
Accession Version	NM_182192.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Protein argonaute
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_182192.2. COMPLETENESS: incomplete on both ends.

ATGTTCCCTC TGCCTGTACA CAATGGTCCT AGACTTGGAA AATTATCGAT TTTCGAAATG 
CCCGGCGATT CGCTGACATC GTCGTCTTTC ATGCCAGATG GTGGCGCAGA AACGTCGAGC 
TCTTCACAGT TGGGCGGCTC GGCTCACGGA GCAATTGGCA CAAAACCCGA CGCAGGCGTC 
CAATTCCAGT GCCCAGTCCG TCCGAACCAT GGAGTCGAGG GCCGATCCAT CCTCCTGCGA 
GCCAATCATT TTGCAGTACG GATCCCCGGA GGTTCAGTCC AACATTATCA AATTGACGTC 
TTTCCGGACA AGTGCCCAAG ACGGGTTAAT CGCGAGGTAA TCGGCTGCCT GATCTCCTCC 
TTCTCAAAAT ATTTCACCAA TATTCGACCA GTCTACGATG GAAAGCGTAA CATGTACACG 
CGGGAGCCCC TACCAATTGG CACGGAGCCC ATGAACTTTG AGGTGACGCT GCCTGGAGAT 
TCAGCAGTGG AACGGAAGTT CTCAGTGACG ATGAAGTGGA TCGGTCAGGT CTGTCTGTCA 
GCTCTGGACG ATGCCATGGA AGGTAGAGTG CGGCAGGTTC CACATGAAGC CGTTCAGTCG 
ATCGATGTGA TTCTTCGCCA CTTGCCAAGT TTGAAGTACA CCCCGGTTGG AAGGTCGTTT 
TTCACACCTC CAGGCGTCAT GAAGCCCGGC ATGCAAATGC ACCAGGAGAG CAAACTCGGT 
GGAGGCCGCG AAGTATGGTT TGGATTCCAT CAATCCGTCC GACCATCCCA ATGGAAGATG 
ATGCTCAACA TTGACGTCTC CGCCACGGCC TTTTACCGCG CGATGCCAGT CATCGAATTC 
GTCGCCGAGG TGCTCGAGCT CCCAGTCCAA GCTCTCGCCG AGCGTCGGGC CCTCTCCGAT 
GCTCAACGCG TCAAATTCAC CAAAGAGATC CGTGGACTCA AGATTGAGAT CACCCATTGT 
GGAGCCGTGC GCCGGAAGTA CAGAGTTTGC AACGTCACAC GCCGCCCGGC CCAGACCCAG 
ACCTTCCCAT TGCAACTGGA GACTGGCCAA ACTATTGAGT GTACTGTAGC CAAGTACTTC 
TTTGACAAGT ACCGTATCCA GCTGAAGTAC CCCCACCTTC CATGCCTCCA AGTCGGTCAG 
GAGCAGAAGC ATACGTACCT GCCACCAGAG GTCTGCGATA TTGTTCCAGG ACAGCGTTGT 
CTGAAGAAGC TCACCGATGT GCAGACGTCC ACCATGATCA AGGCCACGGC TCGTTCCGCT 
CCAGAGCGTG AGCGTGAGAT TTGTAAGCTA GTAAGCAAGG CTGAGCTCTC CGCGGATCCA 
TTTGCTCATG AATTCGGAAT CACTATCAAT CCAGCAATGA CTGAAGTCAA GGGACGTGTG 
CTCTCGGCTC CGAAGCTTCT CTATGGAGGT CGTCATCGTG CCACAACTGC CCTTCCGAAC 
CAGGGAGTCT GGGATATGCG CGGGAAGCAG TTCCACACTG GAATGGAAGT CCGTACCTGG 
GCGATTGCAT GCTTTGCTCA GCAATCTCAT GTCAAGGAAA ATGATCTCAG GATGTTCACG 
ACTCAATTGC AAAGGATCTC TACCGACGCT GGAATGCCAA TCATCGGTAC CCCTATGTTC 
TGCAAATATG CTTCTGGAGT GGAGCAGGTG GAGCCGATGT TCAAGTACTT GAAGCAGACC 
TATTCGGCGA TTCAGTTGAT TGTGGTGGTG CTGCCAGGAA AGACTCCGAT CTATGCCGAA 
GTGAAACGCG TCGGAGACAC CGTCCTCGGA ATCGCCACCC AGTGCGTCCA GGCCAAGAAC 
GCCATCCGAA CCACCCCGCA GACACTCTCC AACCTCTGTC TCAAGATGAA TGTGAAGCTT 
GGTGGAGTCA ACAGCATCCT CCTGCCAAAC GTGCGTCCAC GGATCTTCAA CGAGCCCGTC 
ATCTTCCTGG GTTGCGACAT CACCCATCCA GCAGCCGGTG ATACCCGTAA GCCATCCATC 
GCCGCAGTGG TAGGATCTAT GGATGCTCAC CCGAGTAGAT ATGCGGCAAC AGTTCGGGTC 
CAGCAGCACC GTCAGGAGAT CATTACGGAT CTGACATATA TGGTTCGAGA GTTGCTTGTC 
CAGTTCTACC GTAACACGAG GTTCAAGCCA GCCCGAATTG TGGTCTACAG AGATGGAGTG 
TCTGAGGGTC AACTCTTCAA TGTGCTCCAA TATGAGCTCC GCGCAATTCG TGAAGCTTGT 
GTGATGCTTG AGAGTGGATA CCAGCCAGGA ATCACATTTA TCGCTGTGCA AAAGAGACAT 
CACACAAGGC TCTTCGCCGC GGATAAAGCT GATCAAGTAG GAAAAGCATT CAACATCCCA 
CCAGGAACAA CTGTAGATGT GGGAATCACG CATCCAACGG AATTCGACTT CTTCCTCTGC 
TCCCACGCTG GTATCCAAGG AACATCCCGA CCATCTCACT ACCATGTGCT CTGGGATGAC 
AATGATCTGA CGGCCGACGA GCTTCAACAG TTGACCTACC AAATGTGTCA TACCTATGTA 
AGATGCACGA GGTCTGTGTC GATCCCGGCG CCGGCGTACT ATGCACATCT GGTGGCGTTC 
CGTGCTCGTT ATCACTTGGT CGATAGGGAT CATGGATCCG GAGAGGAAGG ATCACAACCT 
TCGGGGACGT CGTCGGAGGA TACAACATTG TCAAGCATGG CCAAGGCCGT ACAGGTTCAT 
CCCGACTCGA ACAACGTCAT GTATTTTGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_871992.1
CDS	1..2733
Translation	MFPLPVHNGPRLGKLSIFEMPGDSLTSSSFMPDGGAETSSSSQLGGSAHGAIGTKPDAGVQFQCPVRPNHGVEGRSILLRANHFAVRIPGGSVQHYQIDVFPDKCPRRVNREVIGCLISSFSKYFTNIRPVYDGKRNMYTREPLPIGTEPMNFEVTLPGDSAVERKFSVTMKWIGQVCLSALDDAMEGRVRQVPHEAVQSIDVILRHLPSLKYTPVGRSFFTPPGVMKPGMQMHQESKLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYRAMPVIEFVAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGAVRRKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFFDKYRIQLKYPHLPCLQVGQEQKHTYLPPEVCDIVPGQRCLKKLTDVQTSTMIKATARSAPEREREICKLVSKAELSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRHRATTALPNQGVWDMRGKQFHTGMEVRTWAIACFAQQSHVKENDLRMFTTQLQRISTDAGMPIIGTPMFCKYASGVEQVEPMFKYLKQTYSAIQLIVVVLPGKTPIYAEVKRVGDTVLGIATQCVQAKNAIRTTPQTLSNLCLKMNVKLGGVNSILLPNVRPRIFNEPVIFLGCDITHPAAGDTRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIITDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQLFNVLQYELRAIREACVMLESGYQPGITFIAVQKRHHTRLFAADKADQVGKAFNIPPGTTVDVGITHPTEFDFFLCSHAGIQGTSRPSHYHVLWDDNDLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDRDHGSGEEGSQPSGTSSEDTTLSSMAKAVQVHPDSNNVMYFA

Target ORF information:

RefSeq Version	NM_182192.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein argonaute (alg-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182192.3

ORF Insert Sequence:

ATGTTCCCTC TGCCTGTACA CAATGGTCCT AGACTTGGAA AATTATCGAT TTTCGAAATG 
CCCGGCGATT CGCTGACATC GTCGTCTTTC ATGCCAGATG GTGGCGCAGA AACGTCGAGC 
TCTTCACAGT TGGGCGGCTC GGCTCACGGA GCAATTGGCA CAAAACCCGA CGCAGGCGTC 
CAATTCCAGT GCCCAGTCCG TCCGAACCAT GGAGTCGAGG GCCGATCCAT CCTCCTGCGA 
GCCAATCATT TTGCAGTACG GATCCCCGGA GGTTCAGTCC AACATTATCA AATTGACGTC 
TTTCCGGACA AGTGCCCAAG ACGGGTTAAT CGCGAGGTAA TCGGCTGCCT GATCTCCTCC 
TTCTCAAAAT ATTTCACCAA TATTCGACCA GTCTACGATG GAAAGCGTAA CATGTACACG 
CGGGAGCCCC TACCAATTGG CACGGAGCCC ATGAACTTTG AGGTGACGCT GCCTGGAGAT 
TCAGCAGTGG AACGGAAGTT CTCAGTGACG ATGAAGTGGA TCGGTCAGGT CTGTCTGTCA 
GCTCTGGACG ATGCCATGGA AGGTAGAGTG CGGCAGGTTC CACATGAAGC CGTTCAGTCG 
ATCGATGTGA TTCTTCGCCA CTTGCCAAGT TTGAAGTACA CCCCGGTTGG AAGGTCGTTT 
TTCACACCTC CAGGCGTCAT GAAGCCCGGC ATGCAAATGC ACCAGGAGAG CAAACTCGGT 
GGAGGCCGCG AAGTATGGTT TGGATTCCAT CAATCCGTCC GACCATCCCA ATGGAAGATG 
ATGCTCAACA TTGACGTCTC CGCCACGGCC TTTTACCGCG CGATGCCAGT CATCGAATTC 
GTCGCCGAGG TGCTCGAGCT CCCAGTCCAA GCTCTCGCCG AGCGTCGGGC CCTCTCCGAT 
GCTCAACGCG TCAAATTCAC CAAAGAGATC CGTGGACTCA AGATTGAGAT CACCCATTGT 
GGAGCCGTGC GCCGGAAGTA CAGAGTTTGC AACGTCACAC GCCGCCCGGC CCAGACCCAG 
ACCTTCCCAT TGCAACTGGA GACTGGCCAA ACTATTGAGT GTACTGTAGC CAAGTACTTC 
TTTGACAAGT ACCGTATCCA GCTGAAGTAC CCCCACCTTC CATGCCTCCA AGTCGGTCAG 
GAGCAGAAGC ATACGTACCT GCCACCAGAG GTCTGCGATA TTGTTCCAGG ACAGCGTTGT 
CTGAAGAAGC TCACCGATGT GCAGACGTCC ACCATGATCA AGGCCACGGC TCGTTCCGCT 
CCAGAGCGTG AGCGTGAGAT TTGTAAGCTA GTAAGCAAGG CTGAGCTCTC CGCGGATCCA 
TTTGCTCATG AATTCGGAAT CACTATCAAT CCAGCAATGA CTGAAGTCAA GGGACGTGTG 
CTCTCGGCTC CGAAGCTTCT CTATGGAGGT CGTCATCGTG CCACAACTGC CCTTCCGAAC 
CAGGGAGTCT GGGATATGCG CGGGAAGCAG TTCCACACTG GAATGGAAGT CCGTACCTGG 
GCGATTGCAT GCTTTGCTCA GCAATCTCAT GTCAAGGAAA ATGATCTCAG GATGTTCACG 
ACTCAATTGC AAAGGATCTC TACCGACGCT GGAATGCCAA TCATCGGTAC CCCTATGTTC 
TGCAAATATG CTTCTGGAGT GGAGCAGGTG GAGCCGATGT TCAAGTACTT GAAGCAGACC 
TATTCGGCGA TTCAGTTGAT TGTGGTGGTG CTGCCAGGAA AGACTCCGAT CTATGCCGAA 
GTGAAACGCG TCGGAGACAC CGTCCTCGGA ATCGCCACCC AGTGCGTCCA GGCCAAGAAC 
GCCATCCGAA CCACCCCGCA GACACTCTCC AACCTCTGTC TCAAGATGAA TGTGAAGCTT 
GGTGGAGTCA ACAGCATCCT CCTGCCAAAC GTGCGTCCAC GGATCTTCAA CGAGCCCGTC 
ATCTTCCTGG GTTGCGACAT CACCCATCCA GCAGCCGGTG ATACCCGTAA GCCATCCATC 
GCCGCAGTGG TAGGATCTAT GGATGCTCAC CCGAGTAGAT ATGCGGCAAC AGTTCGGGTC 
CAGCAGCACC GTCAGGAGAT CATTACGGAT CTGACATATA TGGTTCGAGA GTTGCTTGTC 
CAGTTCTACC GTAACACGAG GTTCAAGCCA GCCCGAATTG TGGTCTACAG AGATGGAGTG 
TCTGAGGGTC AACTCTTCAA TGTGCTCCAA TATGAGCTCC GCGCAATTCG TGAAGCTTGT 
GTGATGCTTG AGAGTGGATA CCAGCCAGGA ATCACATTTA TCGCTGTGCA AAAGAGACAT 
CACACAAGGC TCTTCGCCGC GGATAAAGCT GATCAAGTAG GAAAAGCATT CAACATCCCA 
CCAGGAACAA CTGTAGATGT GGGAATCACG CATCCAACGG AATTCGACTT CTTCCTCTGC 
TCCCACGCTG GTATCCAAGG AACATCCCGA CCATCTCACT ACCATGTGCT CTGGGATGAC 
AATGATCTGA CGGCCGACGA GCTTCAACAG TTGACCTACC AAATGTGTCA TACCTATGTA 
AGATGCACGA GGTCTGTGTC GATCCCGGCG CCGGCGTACT ATGCACATCT GGTGGCGTTC 
CGTGCTCGTT ATCACTTGGT CGATAGGGAT CATGGATCCG GAGAGGAAGG ATCACAACCT 
TCGGGGACGT CGTCGGAGGA TACAACATTG TCAAGCATGG CCAAGGCCGT ACAGGTTCAT 
CCCGACTCGA ACAACGTCAT GTATTTTGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alg-2 ( NM_182192.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_871992.1 Caenorhabditis elegans Protein argonaute (alg-2), mRNA.

Price & Availability↑

CloneID	OCa339395
Clone ID Related Accession (Same CDS sequence)	NM_182192.3 , NM_182192.4
Accession Version	NM_182192.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein argonaute
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_182192.3.

ATGTTCCCTC TGCCTGTACA CAATGGTCCT AGACTTGGAA AATTATCGAT TTTCGAAATG 
CCCGGCGATT CGCTGACATC GTCGTCTTTC ATGCCAGATG GTGGCGCAGA AACGTCGAGC 
TCTTCACAGT TGGGCGGCTC GGCTCACGGA GCAATTGGCA CAAAACCCGA CGCAGGCGTC 
CAATTCCAGT GCCCAGTCCG TCCGAACCAT GGAGTCGAGG GCCGATCCAT CCTCCTGCGA 
GCCAATCATT TTGCAGTACG GATCCCCGGA GGTTCAGTCC AACATTATCA AATTGACGTC 
TTTCCGGACA AGTGCCCAAG ACGGGTTAAT CGCGAGGTAA TCGGCTGCCT GATCTCCTCC 
TTCTCAAAAT ATTTCACCAA TATTCGACCA GTCTACGATG GAAAGCGTAA CATGTACACG 
CGGGAGCCCC TACCAATTGG CACGGAGCCC ATGAACTTTG AGGTGACGCT GCCTGGAGAT 
TCAGCAGTGG AACGGAAGTT CTCAGTGACG ATGAAGTGGA TCGGTCAGGT CTGTCTGTCA 
GCTCTGGACG ATGCCATGGA AGGTAGAGTG CGGCAGGTTC CACATGAAGC CGTTCAGTCG 
ATCGATGTGA TTCTTCGCCA CTTGCCAAGT TTGAAGTACA CCCCGGTTGG AAGGTCGTTT 
TTCACACCTC CAGGCGTCAT GAAGCCCGGC ATGCAAATGC ACCAGGAGAG CAAACTCGGT 
GGAGGCCGCG AAGTATGGTT TGGATTCCAT CAATCCGTCC GACCATCCCA ATGGAAGATG 
ATGCTCAACA TTGACGTCTC CGCCACGGCC TTTTACCGCG CGATGCCAGT CATCGAATTC 
GTCGCCGAGG TGCTCGAGCT CCCAGTCCAA GCTCTCGCCG AGCGTCGGGC CCTCTCCGAT 
GCTCAACGCG TCAAATTCAC CAAAGAGATC CGTGGACTCA AGATTGAGAT CACCCATTGT 
GGAGCCGTGC GCCGGAAGTA CAGAGTTTGC AACGTCACAC GCCGCCCGGC CCAGACCCAG 
ACCTTCCCAT TGCAACTGGA GACTGGCCAA ACTATTGAGT GTACTGTAGC CAAGTACTTC 
TTTGACAAGT ACCGTATCCA GCTGAAGTAC CCCCACCTTC CATGCCTCCA AGTCGGTCAG 
GAGCAGAAGC ATACGTACCT GCCACCAGAG GTCTGCGATA TTGTTCCAGG ACAGCGTTGT 
CTGAAGAAGC TCACCGATGT GCAGACGTCC ACCATGATCA AGGCCACGGC TCGTTCCGCT 
CCAGAGCGTG AGCGTGAGAT TTGTAAGCTA GTAAGCAAGG CTGAGCTCTC CGCGGATCCA 
TTTGCTCATG AATTCGGAAT CACTATCAAT CCAGCAATGA CTGAAGTCAA GGGACGTGTG 
CTCTCGGCTC CGAAGCTTCT CTATGGAGGT CGTCATCGTG CCACAACTGC CCTTCCGAAC 
CAGGGAGTCT GGGATATGCG CGGGAAGCAG TTCCACACTG GAATGGAAGT CCGTACCTGG 
GCGATTGCAT GCTTTGCTCA GCAATCTCAT GTCAAGGAAA ATGATCTCAG GATGTTCACG 
ACTCAATTGC AAAGGATCTC TACCGACGCT GGAATGCCAA TCATCGGTAC CCCTATGTTC 
TGCAAATATG CTTCTGGAGT GGAGCAGGTG GAGCCGATGT TCAAGTACTT GAAGCAGACC 
TATTCGGCGA TTCAGTTGAT TGTGGTGGTG CTGCCAGGAA AGACTCCGAT CTATGCCGAA 
GTGAAACGCG TCGGAGACAC CGTCCTCGGA ATCGCCACCC AGTGCGTCCA GGCCAAGAAC 
GCCATCCGAA CCACCCCGCA GACACTCTCC AACCTCTGTC TCAAGATGAA TGTGAAGCTT 
GGTGGAGTCA ACAGCATCCT CCTGCCAAAC GTGCGTCCAC GGATCTTCAA CGAGCCCGTC 
ATCTTCCTGG GTTGCGACAT CACCCATCCA GCAGCCGGTG ATACCCGTAA GCCATCCATC 
GCCGCAGTGG TAGGATCTAT GGATGCTCAC CCGAGTAGAT ATGCGGCAAC AGTTCGGGTC 
CAGCAGCACC GTCAGGAGAT CATTACGGAT CTGACATATA TGGTTCGAGA GTTGCTTGTC 
CAGTTCTACC GTAACACGAG GTTCAAGCCA GCCCGAATTG TGGTCTACAG AGATGGAGTG 
TCTGAGGGTC AACTCTTCAA TGTGCTCCAA TATGAGCTCC GCGCAATTCG TGAAGCTTGT 
GTGATGCTTG AGAGTGGATA CCAGCCAGGA ATCACATTTA TCGCTGTGCA AAAGAGACAT 
CACACAAGGC TCTTCGCCGC GGATAAAGCT GATCAAGTAG GAAAAGCATT CAACATCCCA 
CCAGGAACAA CTGTAGATGT GGGAATCACG CATCCAACGG AATTCGACTT CTTCCTCTGC 
TCCCACGCTG GTATCCAAGG AACATCCCGA CCATCTCACT ACCATGTGCT CTGGGATGAC 
AATGATCTGA CGGCCGACGA GCTTCAACAG TTGACCTACC AAATGTGTCA TACCTATGTA 
AGATGCACGA GGTCTGTGTC GATCCCGGCG CCGGCGTACT ATGCACATCT GGTGGCGTTC 
CGTGCTCGTT ATCACTTGGT CGATAGGGAT CATGGATCCG GAGAGGAAGG ATCACAACCT 
TCGGGGACGT CGTCGGAGGA TACAACATTG TCAAGCATGG CCAAGGCCGT ACAGGTTCAT 
CCCGACTCGA ACAACGTCAT GTATTTTGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_871992.1
CDS	41..2773
Translation	MFPLPVHNGPRLGKLSIFEMPGDSLTSSSFMPDGGAETSSSSQLGGSAHGAIGTKPDAGVQFQCPVRPNHGVEGRSILLRANHFAVRIPGGSVQHYQIDVFPDKCPRRVNREVIGCLISSFSKYFTNIRPVYDGKRNMYTREPLPIGTEPMNFEVTLPGDSAVERKFSVTMKWIGQVCLSALDDAMEGRVRQVPHEAVQSIDVILRHLPSLKYTPVGRSFFTPPGVMKPGMQMHQESKLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYRAMPVIEFVAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGAVRRKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFFDKYRIQLKYPHLPCLQVGQEQKHTYLPPEVCDIVPGQRCLKKLTDVQTSTMIKATARSAPEREREICKLVSKAELSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRHRATTALPNQGVWDMRGKQFHTGMEVRTWAIACFAQQSHVKENDLRMFTTQLQRISTDAGMPIIGTPMFCKYASGVEQVEPMFKYLKQTYSAIQLIVVVLPGKTPIYAEVKRVGDTVLGIATQCVQAKNAIRTTPQTLSNLCLKMNVKLGGVNSILLPNVRPRIFNEPVIFLGCDITHPAAGDTRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIITDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQLFNVLQYELRAIREACVMLESGYQPGITFIAVQKRHHTRLFAADKADQVGKAFNIPPGTTVDVGITHPTEFDFFLCSHAGIQGTSRPSHYHVLWDDNDLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDRDHGSGEEGSQPSGTSSEDTTLSSMAKAVQVHPDSNNVMYFA

Target ORF information:

RefSeq Version	NM_182192.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein argonaute (alg-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182192.4

ORF Insert Sequence:

ATGTTCCCTC TGCCTGTACA CAATGGTCCT AGACTTGGAA AATTATCGAT TTTCGAAATG 
CCCGGCGATT CGCTGACATC GTCGTCTTTC ATGCCAGATG GTGGCGCAGA AACGTCGAGC 
TCTTCACAGT TGGGCGGCTC GGCTCACGGA GCAATTGGCA CAAAACCCGA CGCAGGCGTC 
CAATTCCAGT GCCCAGTCCG TCCGAACCAT GGAGTCGAGG GCCGATCCAT CCTCCTGCGA 
GCCAATCATT TTGCAGTACG GATCCCCGGA GGTTCAGTCC AACATTATCA AATTGACGTC 
TTTCCGGACA AGTGCCCAAG ACGGGTTAAT CGCGAGGTAA TCGGCTGCCT GATCTCCTCC 
TTCTCAAAAT ATTTCACCAA TATTCGACCA GTCTACGATG GAAAGCGTAA CATGTACACG 
CGGGAGCCCC TACCAATTGG CACGGAGCCC ATGAACTTTG AGGTGACGCT GCCTGGAGAT 
TCAGCAGTGG AACGGAAGTT CTCAGTGACG ATGAAGTGGA TCGGTCAGGT CTGTCTGTCA 
GCTCTGGACG ATGCCATGGA AGGTAGAGTG CGGCAGGTTC CACATGAAGC CGTTCAGTCG 
ATCGATGTGA TTCTTCGCCA CTTGCCAAGT TTGAAGTACA CCCCGGTTGG AAGGTCGTTT 
TTCACACCTC CAGGCGTCAT GAAGCCCGGC ATGCAAATGC ACCAGGAGAG CAAACTCGGT 
GGAGGCCGCG AAGTATGGTT TGGATTCCAT CAATCCGTCC GACCATCCCA ATGGAAGATG 
ATGCTCAACA TTGACGTCTC CGCCACGGCC TTTTACCGCG CGATGCCAGT CATCGAATTC 
GTCGCCGAGG TGCTCGAGCT CCCAGTCCAA GCTCTCGCCG AGCGTCGGGC CCTCTCCGAT 
GCTCAACGCG TCAAATTCAC CAAAGAGATC CGTGGACTCA AGATTGAGAT CACCCATTGT 
GGAGCCGTGC GCCGGAAGTA CAGAGTTTGC AACGTCACAC GCCGCCCGGC CCAGACCCAG 
ACCTTCCCAT TGCAACTGGA GACTGGCCAA ACTATTGAGT GTACTGTAGC CAAGTACTTC 
TTTGACAAGT ACCGTATCCA GCTGAAGTAC CCCCACCTTC CATGCCTCCA AGTCGGTCAG 
GAGCAGAAGC ATACGTACCT GCCACCAGAG GTCTGCGATA TTGTTCCAGG ACAGCGTTGT 
CTGAAGAAGC TCACCGATGT GCAGACGTCC ACCATGATCA AGGCCACGGC TCGTTCCGCT 
CCAGAGCGTG AGCGTGAGAT TTGTAAGCTA GTAAGCAAGG CTGAGCTCTC CGCGGATCCA 
TTTGCTCATG AATTCGGAAT CACTATCAAT CCAGCAATGA CTGAAGTCAA GGGACGTGTG 
CTCTCGGCTC CGAAGCTTCT CTATGGAGGT CGTCATCGTG CCACAACTGC CCTTCCGAAC 
CAGGGAGTCT GGGATATGCG CGGGAAGCAG TTCCACACTG GAATGGAAGT CCGTACCTGG 
GCGATTGCAT GCTTTGCTCA GCAATCTCAT GTCAAGGAAA ATGATCTCAG GATGTTCACG 
ACTCAATTGC AAAGGATCTC TACCGACGCT GGAATGCCAA TCATCGGTAC CCCTATGTTC 
TGCAAATATG CTTCTGGAGT GGAGCAGGTG GAGCCGATGT TCAAGTACTT GAAGCAGACC 
TATTCGGCGA TTCAGTTGAT TGTGGTGGTG CTGCCAGGAA AGACTCCGAT CTATGCCGAA 
GTGAAACGCG TCGGAGACAC CGTCCTCGGA ATCGCCACCC AGTGCGTCCA GGCCAAGAAC 
GCCATCCGAA CCACCCCGCA GACACTCTCC AACCTCTGTC TCAAGATGAA TGTGAAGCTT 
GGTGGAGTCA ACAGCATCCT CCTGCCAAAC GTGCGTCCAC GGATCTTCAA CGAGCCCGTC 
ATCTTCCTGG GTTGCGACAT CACCCATCCA GCAGCCGGTG ATACCCGTAA GCCATCCATC 
GCCGCAGTGG TAGGATCTAT GGATGCTCAC CCGAGTAGAT ATGCGGCAAC AGTTCGGGTC 
CAGCAGCACC GTCAGGAGAT CATTACGGAT CTGACATATA TGGTTCGAGA GTTGCTTGTC 
CAGTTCTACC GTAACACGAG GTTCAAGCCA GCCCGAATTG TGGTCTACAG AGATGGAGTG 
TCTGAGGGTC AACTCTTCAA TGTGCTCCAA TATGAGCTCC GCGCAATTCG TGAAGCTTGT 
GTGATGCTTG AGAGTGGATA CCAGCCAGGA ATCACATTTA TCGCTGTGCA AAAGAGACAT 
CACACAAGGC TCTTCGCCGC GGATAAAGCT GATCAAGTAG GAAAAGCATT CAACATCCCA 
CCAGGAACAA CTGTAGATGT GGGAATCACG CATCCAACGG AATTCGACTT CTTCCTCTGC 
TCCCACGCTG GTATCCAAGG AACATCCCGA CCATCTCACT ACCATGTGCT CTGGGATGAC 
AATGATCTGA CGGCCGACGA GCTTCAACAG TTGACCTACC AAATGTGTCA TACCTATGTA 
AGATGCACGA GGTCTGTGTC GATCCCGGCG CCGGCGTACT ATGCACATCT GGTGGCGTTC 
CGTGCTCGTT ATCACTTGGT CGATAGGGAT CATGGATCCG GAGAGGAAGG ATCACAACCT 
TCGGGGACGT CGTCGGAGGA TACAACATTG TCAAGCATGG CCAAGGCCGT ACAGGTTCAT 
CCCGACTCGA ACAACGTCAT GTATTTTGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alg-2 ( NM_061436.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_493837.1 Caenorhabditis elegans Protein argonaute (alg-2), partial mRNA.

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CloneID	OCa339396
Clone ID Related Accession (Same CDS sequence)	NM_061436.3 , NM_061436.4
Accession Version	NM_061436.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Protein argonaute
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_061436.2. COMPLETENESS: incomplete on both ends.

ATGCCCGGCG ATTCGCTGAC ATCGTCGTCT TTCATGCCAG ATGGTGGCGC AGAAACGTCG 
AGCTCTTCAC AGTTGGGCGG CTCGGCTCAC GGAGCAATTG GCACAAAACC CGACGCAGGC 
GTCCAATTCC AGTGCCCAGT CCGTCCGAAC CATGGAGTCG AGGGCCGATC CATCCTCCTG 
CGAGCCAATC ATTTTGCAGT ACGGATCCCC GGAGGTTCAG TCCAACATTA TCAAATTGAC 
GTCTTTCCGG ACAAGTGCCC AAGACGGGTT AATCGCGAGG TAATCGGCTG CCTGATCTCC 
TCCTTCTCAA AATATTTCAC CAATATTCGA CCAGTCTACG ATGGAAAGCG TAACATGTAC 
ACGCGGGAGC CCCTACCAAT TGGCACGGAG CCCATGAACT TTGAGGTGAC GCTGCCTGGA 
GATTCAGCAG TGGAACGGAA GTTCTCAGTG ACGATGAAGT GGATCGGTCA GGTCTGTCTG 
TCAGCTCTGG ACGATGCCAT GGAAGGTAGA GTGCGGCAGG TTCCACATGA AGCCGTTCAG 
TCGATCGATG TGATTCTTCG CCACTTGCCA AGTTTGAAGT ACACCCCGGT TGGAAGGTCG 
TTTTTCACAC CTCCAGGCGT CATGAAGCCC GGCATGCAAA TGCACCAGGA GAGCAAACTC 
GGTGGAGGCC GCGAAGTATG GTTTGGATTC CATCAATCCG TCCGACCATC CCAATGGAAG 
ATGATGCTCA ACATTGACGT CTCCGCCACG GCCTTTTACC GCGCGATGCC AGTCATCGAA 
TTCGTCGCCG AGGTGCTCGA GCTCCCAGTC CAAGCTCTCG CCGAGCGTCG GGCCCTCTCC 
GATGCTCAAC GCGTCAAATT CACCAAAGAG ATCCGTGGAC TCAAGATTGA GATCACCCAT 
TGTGGAGCCG TGCGCCGGAA GTACAGAGTT TGCAACGTCA CACGCCGCCC GGCCCAGACC 
CAGACCTTCC CATTGCAACT GGAGACTGGC CAAACTATTG AGTGTACTGT AGCCAAGTAC 
TTCTTTGACA AGTACCGTAT CCAGCTGAAG TACCCCCACC TTCCATGCCT CCAAGTCGGT 
CAGGAGCAGA AGCATACGTA CCTGCCACCA GAGGTCTGCG ATATTGTTCC AGGACAGCGT 
TGTCTGAAGA AGCTCACCGA TGTGCAGACG TCCACCATGA TCAAGGCCAC GGCTCGTTCC 
GCTCCAGAGC GTGAGCGTGA GATTTGTAAG CTAGTAAGCA AGGCTGAGCT CTCCGCGGAT 
CCATTTGCTC ATGAATTCGG AATCACTATC AATCCAGCAA TGACTGAAGT CAAGGGACGT 
GTGCTCTCGG CTCCGAAGCT TCTCTATGGA GGTCGTCATC GTGCCACAAC TGCCCTTCCG 
AACCAGGGAG TCTGGGATAT GCGCGGGAAG CAGTTCCACA CTGGAATGGA AGTCCGTACC 
TGGGCGATTG CATGCTTTGC TCAGCAATCT CATGTCAAGG AAAATGATCT CAGGATGTTC 
ACGACTCAAT TGCAAAGGAT CTCTACCGAC GCTGGAATGC CAATCATCGG TACCCCTATG 
TTCTGCAAAT ATGCTTCTGG AGTGGAGCAG GTGGAGCCGA TGTTCAAGTA CTTGAAGCAG 
ACCTATTCGG CGATTCAGTT GATTGTGGTG GTGCTGCCAG GAAAGACTCC GATCTATGCC 
GAAGTGAAAC GCGTCGGAGA CACCGTCCTC GGAATCGCCA CCCAGTGCGT CCAGGCCAAG 
AACGCCATCC GAACCACCCC GCAGACACTC TCCAACCTCT GTCTCAAGAT GAATGTGAAG 
CTTGGTGGAG TCAACAGCAT CCTCCTGCCA AACGTGCGTC CACGGATCTT CAACGAGCCC 
GTCATCTTCC TGGGTTGCGA CATCACCCAT CCAGCAGCCG GTGATACCCG TAAGCCATCC 
ATCGCCGCAG TGGTAGGATC TATGGATGCT CACCCGAGTA GATATGCGGC AACAGTTCGG 
GTCCAGCAGC ACCGTCAGGA GATCATTACG GATCTGACAT ATATGGTTCG AGAGTTGCTT 
GTCCAGTTCT ACCGTAACAC GAGGTTCAAG CCAGCCCGAA TTGTGGTCTA CAGAGATGGA 
GTGTCTGAGG GTCAACTCTT CAATGTGCTC CAATATGAGC TCCGCGCAAT TCGTGAAGCT 
TGTGTGATGC TTGAGAGTGG ATACCAGCCA GGAATCACAT TTATCGCTGT GCAAAAGAGA 
CATCACACAA GGCTCTTCGC CGCGGATAAA GCTGATCAAG TAGGAAAAGC ATTCAACATC 
CCACCAGGAA CAACTGTAGA TGTGGGAATC ACGCATCCAA CGGAATTCGA CTTCTTCCTC 
TGCTCCCACG CTGGTATCCA AGGAACATCC CGACCATCTC ACTACCATGT GCTCTGGGAT 
GACAATGATC TGACGGCCGA CGAGCTTCAA CAGTTGACCT ACCAAATGTG TCATACCTAT 
GTAAGATGCA CGAGGTCTGT GTCGATCCCG GCGCCGGCGT ACTATGCACA TCTGGTGGCG 
TTCCGTGCTC GTTATCACTT GGTCGATAGG GATCATGGAT CCGGAGAGGA AGGATCACAA 
CCTTCGGGGA CGTCGTCGGA GGATACAACA TTGTCAAGCA TGGCCAAGGC CGTACAGGTT 
CATCCCGACT CGAACAACGT CATGTATTTT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_493837.1
CDS	1..2676
Translation	MPGDSLTSSSFMPDGGAETSSSSQLGGSAHGAIGTKPDAGVQFQCPVRPNHGVEGRSILLRANHFAVRIPGGSVQHYQIDVFPDKCPRRVNREVIGCLISSFSKYFTNIRPVYDGKRNMYTREPLPIGTEPMNFEVTLPGDSAVERKFSVTMKWIGQVCLSALDDAMEGRVRQVPHEAVQSIDVILRHLPSLKYTPVGRSFFTPPGVMKPGMQMHQESKLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYRAMPVIEFVAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGAVRRKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFFDKYRIQLKYPHLPCLQVGQEQKHTYLPPEVCDIVPGQRCLKKLTDVQTSTMIKATARSAPEREREICKLVSKAELSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRHRATTALPNQGVWDMRGKQFHTGMEVRTWAIACFAQQSHVKENDLRMFTTQLQRISTDAGMPIIGTPMFCKYASGVEQVEPMFKYLKQTYSAIQLIVVVLPGKTPIYAEVKRVGDTVLGIATQCVQAKNAIRTTPQTLSNLCLKMNVKLGGVNSILLPNVRPRIFNEPVIFLGCDITHPAAGDTRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIITDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQLFNVLQYELRAIREACVMLESGYQPGITFIAVQKRHHTRLFAADKADQVGKAFNIPPGTTVDVGITHPTEFDFFLCSHAGIQGTSRPSHYHVLWDDNDLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDRDHGSGEEGSQPSGTSSEDTTLSSMAKAVQVHPDSNNVMYFA

Target ORF information:

RefSeq Version	NM_061436.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein argonaute (alg-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061436.3

ORF Insert Sequence:

ATGCCCGGCG ATTCGCTGAC ATCGTCGTCT TTCATGCCAG ATGGTGGCGC AGAAACGTCG 
AGCTCTTCAC AGTTGGGCGG CTCGGCTCAC GGAGCAATTG GCACAAAACC CGACGCAGGC 
GTCCAATTCC AGTGCCCAGT CCGTCCGAAC CATGGAGTCG AGGGCCGATC CATCCTCCTG 
CGAGCCAATC ATTTTGCAGT ACGGATCCCC GGAGGTTCAG TCCAACATTA TCAAATTGAC 
GTCTTTCCGG ACAAGTGCCC AAGACGGGTT AATCGCGAGG TAATCGGCTG CCTGATCTCC 
TCCTTCTCAA AATATTTCAC CAATATTCGA CCAGTCTACG ATGGAAAGCG TAACATGTAC 
ACGCGGGAGC CCCTACCAAT TGGCACGGAG CCCATGAACT TTGAGGTGAC GCTGCCTGGA 
GATTCAGCAG TGGAACGGAA GTTCTCAGTG ACGATGAAGT GGATCGGTCA GGTCTGTCTG 
TCAGCTCTGG ACGATGCCAT GGAAGGTAGA GTGCGGCAGG TTCCACATGA AGCCGTTCAG 
TCGATCGATG TGATTCTTCG CCACTTGCCA AGTTTGAAGT ACACCCCGGT TGGAAGGTCG 
TTTTTCACAC CTCCAGGCGT CATGAAGCCC GGCATGCAAA TGCACCAGGA GAGCAAACTC 
GGTGGAGGCC GCGAAGTATG GTTTGGATTC CATCAATCCG TCCGACCATC CCAATGGAAG 
ATGATGCTCA ACATTGACGT CTCCGCCACG GCCTTTTACC GCGCGATGCC AGTCATCGAA 
TTCGTCGCCG AGGTGCTCGA GCTCCCAGTC CAAGCTCTCG CCGAGCGTCG GGCCCTCTCC 
GATGCTCAAC GCGTCAAATT CACCAAAGAG ATCCGTGGAC TCAAGATTGA GATCACCCAT 
TGTGGAGCCG TGCGCCGGAA GTACAGAGTT TGCAACGTCA CACGCCGCCC GGCCCAGACC 
CAGACCTTCC CATTGCAACT GGAGACTGGC CAAACTATTG AGTGTACTGT AGCCAAGTAC 
TTCTTTGACA AGTACCGTAT CCAGCTGAAG TACCCCCACC TTCCATGCCT CCAAGTCGGT 
CAGGAGCAGA AGCATACGTA CCTGCCACCA GAGGTCTGCG ATATTGTTCC AGGACAGCGT 
TGTCTGAAGA AGCTCACCGA TGTGCAGACG TCCACCATGA TCAAGGCCAC GGCTCGTTCC 
GCTCCAGAGC GTGAGCGTGA GATTTGTAAG CTAGTAAGCA AGGCTGAGCT CTCCGCGGAT 
CCATTTGCTC ATGAATTCGG AATCACTATC AATCCAGCAA TGACTGAAGT CAAGGGACGT 
GTGCTCTCGG CTCCGAAGCT TCTCTATGGA GGTCGTCATC GTGCCACAAC TGCCCTTCCG 
AACCAGGGAG TCTGGGATAT GCGCGGGAAG CAGTTCCACA CTGGAATGGA AGTCCGTACC 
TGGGCGATTG CATGCTTTGC TCAGCAATCT CATGTCAAGG AAAATGATCT CAGGATGTTC 
ACGACTCAAT TGCAAAGGAT CTCTACCGAC GCTGGAATGC CAATCATCGG TACCCCTATG 
TTCTGCAAAT ATGCTTCTGG AGTGGAGCAG GTGGAGCCGA TGTTCAAGTA CTTGAAGCAG 
ACCTATTCGG CGATTCAGTT GATTGTGGTG GTGCTGCCAG GAAAGACTCC GATCTATGCC 
GAAGTGAAAC GCGTCGGAGA CACCGTCCTC GGAATCGCCA CCCAGTGCGT CCAGGCCAAG 
AACGCCATCC GAACCACCCC GCAGACACTC TCCAACCTCT GTCTCAAGAT GAATGTGAAG 
CTTGGTGGAG TCAACAGCAT CCTCCTGCCA AACGTGCGTC CACGGATCTT CAACGAGCCC 
GTCATCTTCC TGGGTTGCGA CATCACCCAT CCAGCAGCCG GTGATACCCG TAAGCCATCC 
ATCGCCGCAG TGGTAGGATC TATGGATGCT CACCCGAGTA GATATGCGGC AACAGTTCGG 
GTCCAGCAGC ACCGTCAGGA GATCATTACG GATCTGACAT ATATGGTTCG AGAGTTGCTT 
GTCCAGTTCT ACCGTAACAC GAGGTTCAAG CCAGCCCGAA TTGTGGTCTA CAGAGATGGA 
GTGTCTGAGG GTCAACTCTT CAATGTGCTC CAATATGAGC TCCGCGCAAT TCGTGAAGCT 
TGTGTGATGC TTGAGAGTGG ATACCAGCCA GGAATCACAT TTATCGCTGT GCAAAAGAGA 
CATCACACAA GGCTCTTCGC CGCGGATAAA GCTGATCAAG TAGGAAAAGC ATTCAACATC 
CCACCAGGAA CAACTGTAGA TGTGGGAATC ACGCATCCAA CGGAATTCGA CTTCTTCCTC 
TGCTCCCACG CTGGTATCCA AGGAACATCC CGACCATCTC ACTACCATGT GCTCTGGGAT 
GACAATGATC TGACGGCCGA CGAGCTTCAA CAGTTGACCT ACCAAATGTG TCATACCTAT 
GTAAGATGCA CGAGGTCTGT GTCGATCCCG GCGCCGGCGT ACTATGCACA TCTGGTGGCG 
TTCCGTGCTC GTTATCACTT GGTCGATAGG GATCATGGAT CCGGAGAGGA AGGATCACAA 
CCTTCGGGGA CGTCGTCGGA GGATACAACA TTGTCAAGCA TGGCCAAGGC CGTACAGGTT 
CATCCCGACT CGAACAACGT CATGTATTTT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alg-2 ( NM_061436.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_493837.1 Caenorhabditis elegans Protein argonaute (alg-2), mRNA.

Price & Availability↑

CloneID	OCa339396
Clone ID Related Accession (Same CDS sequence)	NM_061436.3 , NM_061436.4
Accession Version	NM_061436.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein argonaute
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_061436.3.

ATGCCCGGCG ATTCGCTGAC ATCGTCGTCT TTCATGCCAG ATGGTGGCGC AGAAACGTCG 
AGCTCTTCAC AGTTGGGCGG CTCGGCTCAC GGAGCAATTG GCACAAAACC CGACGCAGGC 
GTCCAATTCC AGTGCCCAGT CCGTCCGAAC CATGGAGTCG AGGGCCGATC CATCCTCCTG 
CGAGCCAATC ATTTTGCAGT ACGGATCCCC GGAGGTTCAG TCCAACATTA TCAAATTGAC 
GTCTTTCCGG ACAAGTGCCC AAGACGGGTT AATCGCGAGG TAATCGGCTG CCTGATCTCC 
TCCTTCTCAA AATATTTCAC CAATATTCGA CCAGTCTACG ATGGAAAGCG TAACATGTAC 
ACGCGGGAGC CCCTACCAAT TGGCACGGAG CCCATGAACT TTGAGGTGAC GCTGCCTGGA 
GATTCAGCAG TGGAACGGAA GTTCTCAGTG ACGATGAAGT GGATCGGTCA GGTCTGTCTG 
TCAGCTCTGG ACGATGCCAT GGAAGGTAGA GTGCGGCAGG TTCCACATGA AGCCGTTCAG 
TCGATCGATG TGATTCTTCG CCACTTGCCA AGTTTGAAGT ACACCCCGGT TGGAAGGTCG 
TTTTTCACAC CTCCAGGCGT CATGAAGCCC GGCATGCAAA TGCACCAGGA GAGCAAACTC 
GGTGGAGGCC GCGAAGTATG GTTTGGATTC CATCAATCCG TCCGACCATC CCAATGGAAG 
ATGATGCTCA ACATTGACGT CTCCGCCACG GCCTTTTACC GCGCGATGCC AGTCATCGAA 
TTCGTCGCCG AGGTGCTCGA GCTCCCAGTC CAAGCTCTCG CCGAGCGTCG GGCCCTCTCC 
GATGCTCAAC GCGTCAAATT CACCAAAGAG ATCCGTGGAC TCAAGATTGA GATCACCCAT 
TGTGGAGCCG TGCGCCGGAA GTACAGAGTT TGCAACGTCA CACGCCGCCC GGCCCAGACC 
CAGACCTTCC CATTGCAACT GGAGACTGGC CAAACTATTG AGTGTACTGT AGCCAAGTAC 
TTCTTTGACA AGTACCGTAT CCAGCTGAAG TACCCCCACC TTCCATGCCT CCAAGTCGGT 
CAGGAGCAGA AGCATACGTA CCTGCCACCA GAGGTCTGCG ATATTGTTCC AGGACAGCGT 
TGTCTGAAGA AGCTCACCGA TGTGCAGACG TCCACCATGA TCAAGGCCAC GGCTCGTTCC 
GCTCCAGAGC GTGAGCGTGA GATTTGTAAG CTAGTAAGCA AGGCTGAGCT CTCCGCGGAT 
CCATTTGCTC ATGAATTCGG AATCACTATC AATCCAGCAA TGACTGAAGT CAAGGGACGT 
GTGCTCTCGG CTCCGAAGCT TCTCTATGGA GGTCGTCATC GTGCCACAAC TGCCCTTCCG 
AACCAGGGAG TCTGGGATAT GCGCGGGAAG CAGTTCCACA CTGGAATGGA AGTCCGTACC 
TGGGCGATTG CATGCTTTGC TCAGCAATCT CATGTCAAGG AAAATGATCT CAGGATGTTC 
ACGACTCAAT TGCAAAGGAT CTCTACCGAC GCTGGAATGC CAATCATCGG TACCCCTATG 
TTCTGCAAAT ATGCTTCTGG AGTGGAGCAG GTGGAGCCGA TGTTCAAGTA CTTGAAGCAG 
ACCTATTCGG CGATTCAGTT GATTGTGGTG GTGCTGCCAG GAAAGACTCC GATCTATGCC 
GAAGTGAAAC GCGTCGGAGA CACCGTCCTC GGAATCGCCA CCCAGTGCGT CCAGGCCAAG 
AACGCCATCC GAACCACCCC GCAGACACTC TCCAACCTCT GTCTCAAGAT GAATGTGAAG 
CTTGGTGGAG TCAACAGCAT CCTCCTGCCA AACGTGCGTC CACGGATCTT CAACGAGCCC 
GTCATCTTCC TGGGTTGCGA CATCACCCAT CCAGCAGCCG GTGATACCCG TAAGCCATCC 
ATCGCCGCAG TGGTAGGATC TATGGATGCT CACCCGAGTA GATATGCGGC AACAGTTCGG 
GTCCAGCAGC ACCGTCAGGA GATCATTACG GATCTGACAT ATATGGTTCG AGAGTTGCTT 
GTCCAGTTCT ACCGTAACAC GAGGTTCAAG CCAGCCCGAA TTGTGGTCTA CAGAGATGGA 
GTGTCTGAGG GTCAACTCTT CAATGTGCTC CAATATGAGC TCCGCGCAAT TCGTGAAGCT 
TGTGTGATGC TTGAGAGTGG ATACCAGCCA GGAATCACAT TTATCGCTGT GCAAAAGAGA 
CATCACACAA GGCTCTTCGC CGCGGATAAA GCTGATCAAG TAGGAAAAGC ATTCAACATC 
CCACCAGGAA CAACTGTAGA TGTGGGAATC ACGCATCCAA CGGAATTCGA CTTCTTCCTC 
TGCTCCCACG CTGGTATCCA AGGAACATCC CGACCATCTC ACTACCATGT GCTCTGGGAT 
GACAATGATC TGACGGCCGA CGAGCTTCAA CAGTTGACCT ACCAAATGTG TCATACCTAT 
GTAAGATGCA CGAGGTCTGT GTCGATCCCG GCGCCGGCGT ACTATGCACA TCTGGTGGCG 
TTCCGTGCTC GTTATCACTT GGTCGATAGG GATCATGGAT CCGGAGAGGA AGGATCACAA 
CCTTCGGGGA CGTCGTCGGA GGATACAACA TTGTCAAGCA TGGCCAAGGC CGTACAGGTT 
CATCCCGACT CGAACAACGT CATGTATTTT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_493837.1
CDS	3..2678
Translation	MPGDSLTSSSFMPDGGAETSSSSQLGGSAHGAIGTKPDAGVQFQCPVRPNHGVEGRSILLRANHFAVRIPGGSVQHYQIDVFPDKCPRRVNREVIGCLISSFSKYFTNIRPVYDGKRNMYTREPLPIGTEPMNFEVTLPGDSAVERKFSVTMKWIGQVCLSALDDAMEGRVRQVPHEAVQSIDVILRHLPSLKYTPVGRSFFTPPGVMKPGMQMHQESKLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYRAMPVIEFVAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGAVRRKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFFDKYRIQLKYPHLPCLQVGQEQKHTYLPPEVCDIVPGQRCLKKLTDVQTSTMIKATARSAPEREREICKLVSKAELSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRHRATTALPNQGVWDMRGKQFHTGMEVRTWAIACFAQQSHVKENDLRMFTTQLQRISTDAGMPIIGTPMFCKYASGVEQVEPMFKYLKQTYSAIQLIVVVLPGKTPIYAEVKRVGDTVLGIATQCVQAKNAIRTTPQTLSNLCLKMNVKLGGVNSILLPNVRPRIFNEPVIFLGCDITHPAAGDTRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIITDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQLFNVLQYELRAIREACVMLESGYQPGITFIAVQKRHHTRLFAADKADQVGKAFNIPPGTTVDVGITHPTEFDFFLCSHAGIQGTSRPSHYHVLWDDNDLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDRDHGSGEEGSQPSGTSSEDTTLSSMAKAVQVHPDSNNVMYFA

Target ORF information:

RefSeq Version	NM_061436.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein argonaute (alg-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061436.4

ORF Insert Sequence:

ATGCCCGGCG ATTCGCTGAC ATCGTCGTCT TTCATGCCAG ATGGTGGCGC AGAAACGTCG 
AGCTCTTCAC AGTTGGGCGG CTCGGCTCAC GGAGCAATTG GCACAAAACC CGACGCAGGC 
GTCCAATTCC AGTGCCCAGT CCGTCCGAAC CATGGAGTCG AGGGCCGATC CATCCTCCTG 
CGAGCCAATC ATTTTGCAGT ACGGATCCCC GGAGGTTCAG TCCAACATTA TCAAATTGAC 
GTCTTTCCGG ACAAGTGCCC AAGACGGGTT AATCGCGAGG TAATCGGCTG CCTGATCTCC 
TCCTTCTCAA AATATTTCAC CAATATTCGA CCAGTCTACG ATGGAAAGCG TAACATGTAC 
ACGCGGGAGC CCCTACCAAT TGGCACGGAG CCCATGAACT TTGAGGTGAC GCTGCCTGGA 
GATTCAGCAG TGGAACGGAA GTTCTCAGTG ACGATGAAGT GGATCGGTCA GGTCTGTCTG 
TCAGCTCTGG ACGATGCCAT GGAAGGTAGA GTGCGGCAGG TTCCACATGA AGCCGTTCAG 
TCGATCGATG TGATTCTTCG CCACTTGCCA AGTTTGAAGT ACACCCCGGT TGGAAGGTCG 
TTTTTCACAC CTCCAGGCGT CATGAAGCCC GGCATGCAAA TGCACCAGGA GAGCAAACTC 
GGTGGAGGCC GCGAAGTATG GTTTGGATTC CATCAATCCG TCCGACCATC CCAATGGAAG 
ATGATGCTCA ACATTGACGT CTCCGCCACG GCCTTTTACC GCGCGATGCC AGTCATCGAA 
TTCGTCGCCG AGGTGCTCGA GCTCCCAGTC CAAGCTCTCG CCGAGCGTCG GGCCCTCTCC 
GATGCTCAAC GCGTCAAATT CACCAAAGAG ATCCGTGGAC TCAAGATTGA GATCACCCAT 
TGTGGAGCCG TGCGCCGGAA GTACAGAGTT TGCAACGTCA CACGCCGCCC GGCCCAGACC 
CAGACCTTCC CATTGCAACT GGAGACTGGC CAAACTATTG AGTGTACTGT AGCCAAGTAC 
TTCTTTGACA AGTACCGTAT CCAGCTGAAG TACCCCCACC TTCCATGCCT CCAAGTCGGT 
CAGGAGCAGA AGCATACGTA CCTGCCACCA GAGGTCTGCG ATATTGTTCC AGGACAGCGT 
TGTCTGAAGA AGCTCACCGA TGTGCAGACG TCCACCATGA TCAAGGCCAC GGCTCGTTCC 
GCTCCAGAGC GTGAGCGTGA GATTTGTAAG CTAGTAAGCA AGGCTGAGCT CTCCGCGGAT 
CCATTTGCTC ATGAATTCGG AATCACTATC AATCCAGCAA TGACTGAAGT CAAGGGACGT 
GTGCTCTCGG CTCCGAAGCT TCTCTATGGA GGTCGTCATC GTGCCACAAC TGCCCTTCCG 
AACCAGGGAG TCTGGGATAT GCGCGGGAAG CAGTTCCACA CTGGAATGGA AGTCCGTACC 
TGGGCGATTG CATGCTTTGC TCAGCAATCT CATGTCAAGG AAAATGATCT CAGGATGTTC 
ACGACTCAAT TGCAAAGGAT CTCTACCGAC GCTGGAATGC CAATCATCGG TACCCCTATG 
TTCTGCAAAT ATGCTTCTGG AGTGGAGCAG GTGGAGCCGA TGTTCAAGTA CTTGAAGCAG 
ACCTATTCGG CGATTCAGTT GATTGTGGTG GTGCTGCCAG GAAAGACTCC GATCTATGCC 
GAAGTGAAAC GCGTCGGAGA CACCGTCCTC GGAATCGCCA CCCAGTGCGT CCAGGCCAAG 
AACGCCATCC GAACCACCCC GCAGACACTC TCCAACCTCT GTCTCAAGAT GAATGTGAAG 
CTTGGTGGAG TCAACAGCAT CCTCCTGCCA AACGTGCGTC CACGGATCTT CAACGAGCCC 
GTCATCTTCC TGGGTTGCGA CATCACCCAT CCAGCAGCCG GTGATACCCG TAAGCCATCC 
ATCGCCGCAG TGGTAGGATC TATGGATGCT CACCCGAGTA GATATGCGGC AACAGTTCGG 
GTCCAGCAGC ACCGTCAGGA GATCATTACG GATCTGACAT ATATGGTTCG AGAGTTGCTT 
GTCCAGTTCT ACCGTAACAC GAGGTTCAAG CCAGCCCGAA TTGTGGTCTA CAGAGATGGA 
GTGTCTGAGG GTCAACTCTT CAATGTGCTC CAATATGAGC TCCGCGCAAT TCGTGAAGCT 
TGTGTGATGC TTGAGAGTGG ATACCAGCCA GGAATCACAT TTATCGCTGT GCAAAAGAGA 
CATCACACAA GGCTCTTCGC CGCGGATAAA GCTGATCAAG TAGGAAAAGC ATTCAACATC 
CCACCAGGAA CAACTGTAGA TGTGGGAATC ACGCATCCAA CGGAATTCGA CTTCTTCCTC 
TGCTCCCACG CTGGTATCCA AGGAACATCC CGACCATCTC ACTACCATGT GCTCTGGGAT 
GACAATGATC TGACGGCCGA CGAGCTTCAA CAGTTGACCT ACCAAATGTG TCATACCTAT 
GTAAGATGCA CGAGGTCTGT GTCGATCCCG GCGCCGGCGT ACTATGCACA TCTGGTGGCG 
TTCCGTGCTC GTTATCACTT GGTCGATAGG GATCATGGAT CCGGAGAGGA AGGATCACAA 
CCTTCGGGGA CGTCGTCGGA GGATACAACA TTGTCAAGCA TGGCCAAGGC CGTACAGGTT 
CATCCCGACT CGAACAACGT CATGTATTTT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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alg-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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