chs-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	chs-2
Entrez Gene ID	173405
Full Name	CHitin Synthase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001313616.2	NP_001300545.1	Chitin synthase chs-2
NM_061281.6	NP_493682.2	Chitin synthase chs-2
NM_001313616.1	NP_001300545.1	Chitin synthase chs-2
NM_001313615.2	NP_001300544.1	Chitin synthase chs-2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	kkv	NP_996152.1
Caenorhabditis elegans (roundworm)	chs-2	NP_493682.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001748	XP_321336.5

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The chitin synthase gene chs-2 is essential for C. elegans development and responsible for chitin deposition in the pharynx.
Title: The chitin synthase genes chs-1 and chs-2 are essential for C. elegans development and responsible for chitin deposition in the eggshell and pharynx, respectively.

The following chs-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chs-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa339271	NM_061281.6 Latest version!	Caenorhabditis elegans Chitin synthase chs-2 (chs-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa339542	NM_001313615.2 Latest version!	Caenorhabditis elegans Chitin synthase chs-2 (chs-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa339543	NM_001313616.2 Latest version!	Caenorhabditis elegans Chitin synthase chs-2 (chs-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa339543	NM_001313616.1	Caenorhabditis elegans Chitin synthase chs-2 (chs-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chs-2 ( NM_061281.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_493682.2 Caenorhabditis elegans Chitin synthase chs-2 (chs-2), mRNA.

Price & Availability↑

CloneID	OCa339271
Clone ID Related Accession (Same CDS sequence)	NM_061281.6
Accession Version	NM_061281.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Chitin synthase chs-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_061281.5.

ATGATGAACA CATTGGACCA TCGGCCCCTT GGCCGGATGG AAACGATGGA GGGGAAACCA 
GATGAAGACG AAGTGCCAAC GTCTTCAAAT TCTGATGCTA AAGGCAAAGG ATACTATTAT 
TCGAGTGGTA CTGTACCTAC AGACGACTCC ACTTTAGAAG AAAAATGTCA ACAGAAGACG 
TTCGATCCAT CGTGTCCGAC ACCTAAAACT CCGGTTATTG TTCCAAATAG GGAATTTGAT 
CCTAACTTCT CAACAGTGAC AGAAAACAAA GGATGGGACA TATTTCGACT GTTACCTCCA 
AAACCCGATC GACTTGGACA TGGATTTTGG CACGATGCAA GTCTTCAAGT TCTTAAACTG 
GCCACTTTTT TGGTGCTCTT CCTGCTCACG CTCGGCTCAG CCGTTGTCGC CAAATCCACA 
TTTATTCTAA TGACGTCGGC AATTGGGTGG GGAGGACAGA CGATAACAAT TTGCAATCAA 
GTAATATCTG AAGCAACTCA GAATACTGTA AAGCTGAAAA ATGCGCATGT GGTGAAATGG 
GTTTGGGCCA CGTTACTGGC ACTGTCGGCT CCTGAAGCAT TGTGCTTTGT GAGATCGATG 
CACCGGACCA TGTTCCGAAA CGTGAAACGC CCGACATTCA TTCAATTTGT ATTTGTGCTC 
ATCATCGAAA CATTTCACAG CATTGGCGTC GGCATTCTCG TTTTCAGAAT ATTTCCAGAC 
TTGGATGCAG TGACAGCTGC ACAGTTAACC AACGCGATGT GTTTTGTACC AGCGATTTTG 
TCAGTCATCA GTCGAAAACC GAACAAGTCA GCGCTGTTGC TAGTAATCAT TGATTTTGCA 
GCGATTGCTG CACAATCAAG CGGGTTTTGG GCTTTACCGA TGTTCCTGCC AAATCTTCAA 
AAGCATTTGG TGGCTATTCC AGTGTCGCTA ACATTGATTT CTCTTGCATG GTGGCAAAAT 
TTTGTTCATC GAGATTCAGT CTTCCCACCA GTGAGAACAT TAGCCAAATT TGCCCAACGC 
TTGTCAGAAC GTCGCTCAAA AACCTACGCA TTCGTTTCCC TTTGGAAAAT CTGCATCTAC 
GTTGTCTGCT GCTTTCTTTT TATCTCAAGT CGAATGAAAA TTGAAGACAT GCTCCAAAAA 
GACCCATTCG GAGAAAAGTT GCTCTCTGTC GCAGGCCATG ACATGAATCA GACGCAAATC 
GAAAAGTTTC AATTGAGAAT AAATCAGATG ATTGAGCAAG CAAATCGCGA GGCCGGGTTC 
TACGCAGCAG CCGAAAAAAA GAAGCAGCCT CCCAAAAAAC AACCGAAAGC TGATGAAGCT 
GAGCAAGTGG ATGCTGGCGA ATATATGATG AAAAGGTTCA AGAGATTCAT TGGAGACGCT 
GGAGAAAATG AGGAAGAGGA ACCTGAAGAG GAAGAATTTT CCAGTTATAA TATCTACTCC 
AATTACGTGG AACGTAATCA GCTAACAATG GCTTATGATG CTTTATGGCT TGTAATATTT 
CAATTTGGCG CTGTCTTTGT ATGCTACCAC AGCTCAAAGT TTGCATGTAA AGTAATGATG 
CAACGAATGG GATTTGCTCT TCCAATGGCT CTCAGTGTAC CTGTTACTGT ACTCTTGCTC 
TCCACAAATT GCCGAATGAG ACAGAAAGAT TCTTGTTACG GTACAAATGT GCTAACTGTG 
GAGCTTTTCT GGCAATGTAA TGGTGCATCA ATGTCGCTTG CCGATTTCAT TTTGACTCCT 
CAAACGTGGA TTTGGCTATG CTGGCTGGCT TCCCAGTTTT GGATCACTAT TCATTTATGG 
AATCCAAAGC ATGAAAGATT GGCAAAGAGT GAAAAACTCT TCATTCTTCC CTACTATATT 
GGCGCTTTTG TTGATCAATC CCTCGCATTC AATCGCCGAC GAGATGATAA GGCAAAGATC 
AAGGCGGAAG ATCTGGAATT CGACGCGGAA GACTCTTCAC TCACCTATGA AACGATTCCA 
GGACTCCAGA ATAAGACTCC GCCAAGTGTT TGCTCGGCAA GTAGTTCAAA ACTGGAAAAT 
GGTTTGATAA GGGATAGTGC AAGTTCTGCA GATGCTATCA CGAAGATCTA TGCCTGTGCG 
ACTATGTGGC ATGAGACTGG TGTCGAGATG ACGTGTATGC TAAAATCACT GTTTAGAATG 
GACGAGGATC AATGCGCTCG ACGAAATGCT CAAAAATACC TAAAAGTTAT CGATCCAGAC 
TACTACGAGT TTGAGGCCCA CATCTTTTTC GATGACGCTT ACGATGTAAA CGAGTACGGG 
GAACCTGAAA TCAACAAGTT CGTGAAACAA ATCGTTAATG TCATTGATCA AGCCGCGTCA 
GCTGTCCATC AAACTCAGAT GAGGCTGAAG CCACCCAAAA AGGCGAAAAC TCCATATGGT 
GGAAAGCTAA CTTATATTAT GCCTGGAAAG AACAAACTAT TCATTCATCT GAAGGATAAT 
CAAAAGATCA GACACAGAAA GAGATGGAGT CAAGTGATGT ATTTGTACTA TCTACTTGGT 
TACAGACTAA TGATGAAGGT TGATGATCCA TCAAGAAAAG AAATTATCTC TGAGAATACG 
TTTATATTGA CTCTGGACGG TGATGTTGAC TTTACGCCGT CATCTGTCTA TCTTCTTGTG 
GATCTTATGA AGAAAAATCG AAGACTCGGT GCGGCATGCG GACGAATTCA TCCTAGAGGT 
GATGGTGCAA TGGTGTGGTA TCAGAAGTTT GAGTACGCAA TTGGACATTG GCTGCAAAAA 
GCGACAGAAC ATATGATCGG TTGTGTTATG TGCTCGCCTG GATGCTTCTC GTTGTTTAGA 
GCATATGCTC TGATGGATGA TAATGTCGCA AGGAGGTACG CTCTAAAATC CGAGGAGCCC 
AAGCACTTTA TCCAGTACGA TCAAGGTGAA GATCGTTGGT TATGCACTTT ACTTCTCCAA 
CGAGGTTATC GAGTAGAATA CTGCGCCGCT TCAGACGCTC AGACCTTTGC CCCCGAAGGC 
TTCAATGAGT TTTTCAATCA ACGCCGTCGC TGGATCCCAT CAACAATTTT CAATATTATG 
GATTTACTGA AGGACTATCG AAATGTAGTA AGAGTCAACG AATCTATCTC GATTTGGTAT 
ATCATATATC AACTTGTCAT GCTTATCTCA TCAATCCTCG GTCCTGGAAC TATATTTGTC 
ATGATCATCG GAGCCATCAG TATCTCATTC TCAATTGATA CTCTGATTTC GTTGGTTATC 
GTGTCTATTC CTGTTGTGGT GTTCATTGTC GTCTGTCTTA CTGCCAAGCC TGAACATCAG 
CTAATTTGTG CACAGACCAT TGGTGCGATC TTTGCCATGC TGATGACAGC TGTCGTTGTA 
GGAACATCCC TTCAGCTTCA GAAGGACGGA TTGCTAAGTC CTCACTCTAT GTTCACAGTG 
GCTGTCGCCA CGTCGTTTTT GACTGCCGCA ATACTTCATC CGCTCGAGTT CACCTGCATC 
ATACCCGGAA CCATTTATTT CTTGGCGATT CCATGTATGT ATATGTTACT CCCGATCTAC 
AGTGTGTGCA ACATGCATAC TGTATCCTGG GGTACTCGCG AAGACCCAAG GCCGACGGAG 
AAAAATACTC TGGCTAAGAA AACTCCTGGA AACCTGGAAT CTGGTGATGG AGCCGGAAAT 
TCTGAAAACT GGTGTACCAG GTTCTTGTGC TGTGGTAGGG GTACGGTACA CCCGATGACT 
ATGGTGATCA ATGAGAAGCT CAATGAAGTT ATCAAGAAGG TTGACCGACT AGATCGAAAA 
CATCATCCAT CGTTAGCTCG TCGTGCATCA ATTTTATCAA GCACTGGTGG AACTATTCAA 
ATCGACAAAT GCTCGGAAGC CGATGAGGAT GAGCAAGCGG AAATCGAAGA CGCTCTGGAA 
ATGTCAAATC AAAGTCACGC GGCAAAGAAA AATCAAAAAT GGAAGCAAGC ACAAAGTGAA 
GCCTGGCTTG CGGACAAGGC GTTGAAGAGA GCAGAACGAG AATATTTGGA ACCAGAGGAG 
GAATCATTTT GGAATGATGT AATTGAACGA TATTTGTCTC CATTGATTAT GGACGGGAAG 
GATATGGATC GGCTGAGAGC TGGATTGATT GCTATCCGAA ACTCCCATAC CGTATACTTT 
TTAATGATCA ACATCGTATT CATCATCTCT GTCCTAGTCC TACAAATTCA CAAAGACTGT 
CTTAACATTG AATGGCCTTT GGGACCAAAA TTCAATCACA CCGTACGTCC ATGTTACGCA 
AATCATGATG ATAATCAGAA GGAAGAAGTT TGGGTTATGA CACGTCTTCA GCTGGAACCC 
ATCGGATTAG TTTTCTTGAT TTTCTTTGTT AGTATTCTGG TCATTCAGTT CCTTGCAATG 
TTGTGCCATC GGTTCGGAAC CCTTGCTCAT ATCATCGCCT CCACAGAGCT TTTCTGTTTC 
AGAAAAACAA TGGACAGACT TAGCGAGGAC GAGCTAGTTG CGCAAAATGC TGTGGAAATT 
GCTCGAGAGT TGCAGGCGAT ACGGGGAATT GATGAAAACG CTCATAATAT TGATAACCCA 
ACCGAAGACC GTGGAATATC ACGCCGACGT GTGGTACAGA ATCTTGAATC TTCGCGAAAA 
TCAATGATGA AAAGGAAGAC AGAGACCCTT GACGCAGCAT TCAAGAAGCG ATTCTTCGCC 
CTGTCAAGTG AACAAACACC AGATCCGGCG GGATTTTCAG CCAGAGACAA TTCGAAACGA 
CTTACATTGA GAAAAGGAAC GATTCGAGCA TTAGAGCACA GGAGGGACTC ACTGTTCGGA 
ACTCTCGACA ATCGAAAGGA CGATGAAGTG GACGCAACAT CAATGCGTGG CCCTGCTCAA 
CGCCGCTTGG AACGTCTTTT CACCGCACAA CAAGACCAGA ATAGTCCGAC ATCGGACGGA 
AATCGACGAA AATCGAATAG CCGACCGTGG GATCAGCCAA CATCTTCTGC CACGTCATCA 
GGTGACGTGG AGCTGAGAAG ATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_493682.2
CDS	225..5231
Translation	MMNTLDHRPLGRMETMEGKPDEDEVPTSSNSDAKGKGYYYSSGTVPTDDSTLEEKCQQKTFDPSCPTPKTPVIVPNREFDPNFSTVTENKGWDIFRLLPPKPDRLGHGFWHDASLQVLKLATFLVLFLLTLGSAVVAKSTFILMTSAIGWGGQTITICNQVISEATQNTVKLKNAHVVKWVWATLLALSAPEALCFVRSMHRTMFRNVKRPTFIQFVFVLIIETFHSIGVGILVFRIFPDLDAVTAAQLTNAMCFVPAILSVISRKPNKSALLLVIIDFAAIAAQSSGFWALPMFLPNLQKHLVAIPVSLTLISLAWWQNFVHRDSVFPPVRTLAKFAQRLSERRSKTYAFVSLWKICIYVVCCFLFISSRMKIEDMLQKDPFGEKLLSVAGHDMNQTQIEKFQLRINQMIEQANREAGFYAAAEKKKQPPKKQPKADEAEQVDAGEYMMKRFKRFIGDAGENEEEEPEEEEFSSYNIYSNYVERNQLTMAYDALWLVIFQFGAVFVCYHSSKFACKVMMQRMGFALPMALSVPVTVLLLSTNCRMRQKDSCYGTNVLTVELFWQCNGASMSLADFILTPQTWIWLCWLASQFWITIHLWNPKHERLAKSEKLFILPYYIGAFVDQSLAFNRRRDDKAKIKAEDLEFDAEDSSLTYETIPGLQNKTPPSVCSASSSKLENGLIRDSASSADAITKIYACATMWHETGVEMTCMLKSLFRMDEDQCARRNAQKYLKVIDPDYYEFEAHIFFDDAYDVNEYGEPEINKFVKQIVNVIDQAASAVHQTQMRLKPPKKAKTPYGGKLTYIMPGKNKLFIHLKDNQKIRHRKRWSQVMYLYYLLGYRLMMKVDDPSRKEIISENTFILTLDGDVDFTPSSVYLLVDLMKKNRRLGAACGRIHPRGDGAMVWYQKFEYAIGHWLQKATEHMIGCVMCSPGCFSLFRAYALMDDNVARRYALKSEEPKHFIQYDQGEDRWLCTLLLQRGYRVEYCAASDAQTFAPEGFNEFFNQRRRWIPSTIFNIMDLLKDYRNVVRVNESISIWYIIYQLVMLISSILGPGTIFVMIIGAISISFSIDTLISLVIVSIPVVVFIVVCLTAKPEHQLICAQTIGAIFAMLMTAVVVGTSLQLQKDGLLSPHSMFTVAVATSFLTAAILHPLEFTCIIPGTIYFLAIPCMYMLLPIYSVCNMHTVSWGTREDPRPTEKNTLAKKTPGNLESGDGAGNSENWCTRFLCCGRGTVHPMTMVINEKLNEVIKKVDRLDRKHHPSLARRASILSSTGGTIQIDKCSEADEDEQAEIEDALEMSNQSHAAKKNQKWKQAQSEAWLADKALKRAEREYLEPEEESFWNDVIERYLSPLIMDGKDMDRLRAGLIAIRNSHTVYFLMINIVFIISVLVLQIHKDCLNIEWPLGPKFNHTVRPCYANHDDNQKEEVWVMTRLQLEPIGLVFLIFFVSILVIQFLAMLCHRFGTLAHIIASTELFCFRKTMDRLSEDELVAQNAVEIARELQAIRGIDENAHNIDNPTEDRGISRRRVVQNLESSRKSMMKRKTETLDAAFKKRFFALSSEQTPDPAGFSARDNSKRLTLRKGTIRALEHRRDSLFGTLDNRKDDEVDATSMRGPAQRRLERLFTAQQDQNSPTSDGNRRKSNSRPWDQPTSSATSSGDVELRRF

Target ORF information:

RefSeq Version	NM_061281.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Chitin synthase chs-2 (chs-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061281.6

ORF Insert Sequence:

ATGATGAACA CATTGGACCA TCGGCCCCTT GGCCGGATGG AAACGATGGA GGGGAAACCA 
GATGAAGACG AAGTGCCAAC GTCTTCAAAT TCTGATGCTA AAGGCAAAGG ATACTATTAT 
TCGAGTGGTA CTGTACCTAC AGACGACTCC ACTTTAGAAG AAAAATGTCA ACAGAAGACG 
TTCGATCCAT CGTGTCCGAC ACCTAAAACT CCGGTTATTG TTCCAAATAG GGAATTTGAT 
CCTAACTTCT CAACAGTGAC AGAAAACAAA GGATGGGACA TATTTCGACT GTTACCTCCA 
AAACCCGATC GACTTGGACA TGGATTTTGG CACGATGCAA GTCTTCAAGT TCTTAAACTG 
GCCACTTTTT TGGTGCTCTT CCTGCTCACG CTCGGCTCAG CCGTTGTCGC CAAATCCACA 
TTTATTCTAA TGACGTCGGC AATTGGGTGG GGAGGACAGA CGATAACAAT TTGCAATCAA 
GTAATATCTG AAGCAACTCA GAATACTGTA AAGCTGAAAA ATGCGCATGT GGTGAAATGG 
GTTTGGGCCA CGTTACTGGC ACTGTCGGCT CCTGAAGCAT TGTGCTTTGT GAGATCGATG 
CACCGGACCA TGTTCCGAAA CGTGAAACGC CCGACATTCA TTCAATTTGT ATTTGTGCTC 
ATCATCGAAA CATTTCACAG CATTGGCGTC GGCATTCTCG TTTTCAGAAT ATTTCCAGAC 
TTGGATGCAG TGACAGCTGC ACAGTTAACC AACGCGATGT GTTTTGTACC AGCGATTTTG 
TCAGTCATCA GTCGAAAACC GAACAAGTCA GCGCTGTTGC TAGTAATCAT TGATTTTGCA 
GCGATTGCTG CACAATCAAG CGGGTTTTGG GCTTTACCGA TGTTCCTGCC AAATCTTCAA 
AAGCATTTGG TGGCTATTCC AGTGTCGCTA ACATTGATTT CTCTTGCATG GTGGCAAAAT 
TTTGTTCATC GAGATTCAGT CTTCCCACCA GTGAGAACAT TAGCCAAATT TGCCCAACGC 
TTGTCAGAAC GTCGCTCAAA AACCTACGCA TTCGTTTCCC TTTGGAAAAT CTGCATCTAC 
GTTGTCTGCT GCTTTCTTTT TATCTCAAGT CGAATGAAAA TTGAAGACAT GCTCCAAAAA 
GACCCATTCG GAGAAAAGTT GCTCTCTGTC GCAGGCCATG ACATGAATCA GACGCAAATC 
GAAAAGTTTC AATTGAGAAT AAATCAGATG ATTGAGCAAG CAAATCGCGA GGCCGGGTTC 
TACGCAGCAG CCGAAAAAAA GAAGCAGCCT CCCAAAAAAC AACCGAAAGC TGATGAAGCT 
GAGCAAGTGG ATGCTGGCGA ATATATGATG AAAAGGTTCA AGAGATTCAT TGGAGACGCT 
GGAGAAAATG AGGAAGAGGA ACCTGAAGAG GAAGAATTTT CCAGTTATAA TATCTACTCC 
AATTACGTGG AACGTAATCA GCTAACAATG GCTTATGATG CTTTATGGCT TGTAATATTT 
CAATTTGGCG CTGTCTTTGT ATGCTACCAC AGCTCAAAGT TTGCATGTAA AGTAATGATG 
CAACGAATGG GATTTGCTCT TCCAATGGCT CTCAGTGTAC CTGTTACTGT ACTCTTGCTC 
TCCACAAATT GCCGAATGAG ACAGAAAGAT TCTTGTTACG GTACAAATGT GCTAACTGTG 
GAGCTTTTCT GGCAATGTAA TGGTGCATCA ATGTCGCTTG CCGATTTCAT TTTGACTCCT 
CAAACGTGGA TTTGGCTATG CTGGCTGGCT TCCCAGTTTT GGATCACTAT TCATTTATGG 
AATCCAAAGC ATGAAAGATT GGCAAAGAGT GAAAAACTCT TCATTCTTCC CTACTATATT 
GGCGCTTTTG TTGATCAATC CCTCGCATTC AATCGCCGAC GAGATGATAA GGCAAAGATC 
AAGGCGGAAG ATCTGGAATT CGACGCGGAA GACTCTTCAC TCACCTATGA AACGATTCCA 
GGACTCCAGA ATAAGACTCC GCCAAGTGTT TGCTCGGCAA GTAGTTCAAA ACTGGAAAAT 
GGTTTGATAA GGGATAGTGC AAGTTCTGCA GATGCTATCA CGAAGATCTA TGCCTGTGCG 
ACTATGTGGC ATGAGACTGG TGTCGAGATG ACGTGTATGC TAAAATCACT GTTTAGAATG 
GACGAGGATC AATGCGCTCG ACGAAATGCT CAAAAATACC TAAAAGTTAT CGATCCAGAC 
TACTACGAGT TTGAGGCCCA CATCTTTTTC GATGACGCTT ACGATGTAAA CGAGTACGGG 
GAACCTGAAA TCAACAAGTT CGTGAAACAA ATCGTTAATG TCATTGATCA AGCCGCGTCA 
GCTGTCCATC AAACTCAGAT GAGGCTGAAG CCACCCAAAA AGGCGAAAAC TCCATATGGT 
GGAAAGCTAA CTTATATTAT GCCTGGAAAG AACAAACTAT TCATTCATCT GAAGGATAAT 
CAAAAGATCA GACACAGAAA GAGATGGAGT CAAGTGATGT ATTTGTACTA TCTACTTGGT 
TACAGACTAA TGATGAAGGT TGATGATCCA TCAAGAAAAG AAATTATCTC TGAGAATACG 
TTTATATTGA CTCTGGACGG TGATGTTGAC TTTACGCCGT CATCTGTCTA TCTTCTTGTG 
GATCTTATGA AGAAAAATCG AAGACTCGGT GCGGCATGCG GACGAATTCA TCCTAGAGGT 
GATGGTGCAA TGGTGTGGTA TCAGAAGTTT GAGTACGCAA TTGGACATTG GCTGCAAAAA 
GCGACAGAAC ATATGATCGG TTGTGTTATG TGCTCGCCTG GATGCTTCTC GTTGTTTAGA 
GCATATGCTC TGATGGATGA TAATGTCGCA AGGAGGTACG CTCTAAAATC CGAGGAGCCC 
AAGCACTTTA TCCAGTACGA TCAAGGTGAA GATCGTTGGT TATGCACTTT ACTTCTCCAA 
CGAGGTTATC GAGTAGAATA CTGCGCCGCT TCAGACGCTC AGACCTTTGC CCCCGAAGGC 
TTCAATGAGT TTTTCAATCA ACGCCGTCGC TGGATCCCAT CAACAATTTT CAATATTATG 
GATTTACTGA AGGACTATCG AAATGTAGTA AGAGTCAACG AATCTATCTC GATTTGGTAT 
ATCATATATC AACTTGTCAT GCTTATCTCA TCAATCCTCG GTCCTGGAAC TATATTTGTC 
ATGATCATCG GAGCCATCAG TATCTCATTC TCAATTGATA CTCTGATTTC GTTGGTTATC 
GTGTCTATTC CTGTTGTGGT GTTCATTGTC GTCTGTCTTA CTGCCAAGCC TGAACATCAG 
CTAATTTGTG CACAGACCAT TGGTGCGATC TTTGCCATGC TGATGACAGC TGTCGTTGTA 
GGAACATCCC TTCAGCTTCA GAAGGACGGA TTGCTAAGTC CTCACTCTAT GTTCACAGTG 
GCTGTCGCCA CGTCGTTTTT GACTGCCGCA ATACTTCATC CGCTCGAGTT CACCTGCATC 
ATACCCGGAA CCATTTATTT CTTGGCGATT CCATGTATGT ATATGTTACT CCCGATCTAC 
AGTGTGTGCA ACATGCATAC TGTATCCTGG GGTACTCGCG AAGACCCAAG GCCGACGGAG 
AAAAATACTC TGGCTAAGAA AACTCCTGGA AACCTGGAAT CTGGTGATGG AGCCGGAAAT 
TCTGAAAACT GGTGTACCAG GTTCTTGTGC TGTGGTAGGG GTACGGTACA CCCGATGACT 
ATGGTGATCA ATGAGAAGCT CAATGAAGTT ATCAAGAAGG TTGACCGACT AGATCGAAAA 
CATCATCCAT CGTTAGCTCG TCGTGCATCA ATTTTATCAA GCACTGGTGG AACTATTCAA 
ATCGACAAAT GCTCGGAAGC CGATGAGGAT GAGCAAGCGG AAATCGAAGA CGCTCTGGAA 
ATGTCAAATC AAAGTCACGC GGCAAAGAAA AATCAAAAAT GGAAGCAAGC ACAAAGTGAA 
GCCTGGCTTG CGGACAAGGC GTTGAAGAGA GCAGAACGAG AATATTTGGA ACCAGAGGAG 
GAATCATTTT GGAATGATGT AATTGAACGA TATTTGTCTC CATTGATTAT GGACGGGAAG 
GATATGGATC GGCTGAGAGC TGGATTGATT GCTATCCGAA ACTCCCATAC CGTATACTTT 
TTAATGATCA ACATCGTATT CATCATCTCT GTCCTAGTCC TACAAATTCA CAAAGACTGT 
CTTAACATTG AATGGCCTTT GGGACCAAAA TTCAATCACA CCGTACGTCC ATGTTACGCA 
AATCATGATG ATAATCAGAA GGAAGAAGTT TGGGTTATGA CACGTCTTCA GCTGGAACCC 
ATCGGATTAG TTTTCTTGAT TTTCTTTGTT AGTATTCTGG TCATTCAGTT CCTTGCAATG 
TTGTGCCATC GGTTCGGAAC CCTTGCTCAT ATCATCGCCT CCACAGAGCT TTTCTGTTTC 
AGAAAAACAA TGGACAGACT TAGCGAGGAC GAGCTAGTTG CGCAAAATGC TGTGGAAATT 
GCTCGAGAGT TGCAGGCGAT ACGGGGAATT GATGAAAACG CTCATAATAT TGATAACCCA 
ACCGAAGACC GTGGAATATC ACGCCGACGT GTGGTACAGA ATCTTGAATC TTCGCGAAAA 
TCAATGATGA AAAGGAAGAC AGAGACCCTT GACGCAGCAT TCAAGAAGCG ATTCTTCGCC 
CTGTCAAGTG AACAAACACC AGATCCGGCG GGATTTTCAG CCAGAGACAA TTCGAAACGA 
CTTACATTGA GAAAAGGAAC GATTCGAGCA TTAGAGCACA GGAGGGACTC ACTGTTCGGA 
ACTCTCGACA ATCGAAAGGA CGATGAAGTG GACGCAACAT CAATGCGTGG CCCTGCTCAA 
CGCCGCTTGG AACGTCTTTT CACCGCACAA CAAGACCAGA ATAGTCCGAC ATCGGACGGA 
AATCGACGAA AATCGAATAG CCGACCGTGG GATCAGCCAA CATCTTCTGC CACGTCATCA 
GGTGACGTGG AGCTGAGAAG ATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chs-2 ( NM_001313615.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300544.1 Caenorhabditis elegans Chitin synthase chs-2 (chs-2), partial mRNA.

Price & Availability↑

CloneID	OCa339542
Clone ID Related Accession (Same CDS sequence)	NM_001313615.2
Accession Version	NM_001313615.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Chitin synthase chs-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001313615.1. COMPLETENESS: incomplete on the 5' end.

ATGGATCGAA CGATGGATCG TCGCGATCGC GATGACAATC ATTCAAATTA TTATATAGCT 
GAAGGAACAG TGACAGAAAA CAAAGGATGG GACATATTTC GACTGTTACC TCCAAAACCC 
GATCGACTTG GACATGGATT TTGGCACGAT GCAAGTCTTC AAGTTCTTAA ACTGGCCACT 
TTTTTGGTGC TCTTCCTGCT CACGCTCGGC TCAGCCGTTG TCGCCAAATC CACATTTATT 
CTAATGACGT CGGCAATTGG GTGGGGAGGA CAGACGATAA CAATTTGCAA TCAAGTAATA 
TCTGAAGCAA CTCAGAATAC TGTAAAGCTG AAAAATGCGC ATGTGGTGAA ATGGGTTTGG 
GCCACGTTAC TGGCACTGTC GGCTCCTGAA GCATTGTGCT TTGTGAGATC GATGCACCGG 
ACCATGTTCC GAAACGTGAA ACGCCCGACA TTCATTCAAT TTGTATTTGT GCTCATCATC 
GAAACATTTC ACAGCATTGG CGTCGGCATT CTCGTTTTCA GAATATTTCC AGACTTGGAT 
GCAGTGACAG CTGCACAGTT AACCAACGCG ATGTGTTTTG TACCAGCGAT TTTGTCAGTC 
ATCAGTCGAA AACCGAACAA GTCAGCGCTG TTGCTAGTAA TCATTGATTT TGCAGCGATT 
GCTGCACAAT CAAGCGGGTT TTGGGCTTTA CCGATGTTCC TGCCAAATCT TCAAAAGCAT 
TTGGTGGCTA TTCCAGTGTC GCTAACATTG ATTTCTCTTG CATGGTGGCA AAATTTTGTT 
CATCGAGATT CAGTCTTCCC ACCAGTGAGA ACATTAGCCA AATTTGCCCA ACGCTTGTCA 
GAACGTCGCT CAAAAACCTA CGCATTCGTT TCCCTTTGGA AAATCTGCAT CTACGTTGTC 
TGCTGCTTTC TTTTTATCTC AAGTCGAATG AAAATTGAAG ACATGCTCCA AAAAGACCCA 
TTCGGAGAAA AGTTGCTCTC TGTCGCAGGC CATGACATGA ATCAGACGCA AATCGAAAAG 
TTTCAATTGA GAATAAATCA GATGATTGAG CAAGCAAATC GCGAGGCCGG GTTCTACGCA 
GCAGCCGAAA AAAAGAAGCA GCCTCCCAAA AAACAACCGA AAGCTGATGA AGCTGAGCAA 
GTGGATGCTG GCGAATATAT GATGAAAAGG TTCAAGAGAT TCATTGGAGA CGCTGGAGAA 
AATGAGGAAG AGGAACCTGA AGAGGAAGAA TTTTCCAGTT ATAATATCTA CTCCAATTAC 
GTGGAACGTA ATCAGCTAAC AATGGCTTAT GATGCTTTAT GGCTTGTAAT ATTTCAATTT 
GGCGCTGTCT TTGTATGCTA CCACAGCTCA AAGTTTGCAT GTAAAGTAAT GATGCAACGA 
ATGGGATTTG CTCTTCCAAT GGCTCTCAGT GTACCTGTTA CTGTACTCTT GCTCTCCACA 
AATTGCCGAA TGAGACAGAA AGATTCTTGT TACGGTACAA ATGTGCTAAC TGTGGAGCTT 
TTCTGGCAAT GTAATGGTGC ATCAATGTCG CTTGCCGATT TCATTTTGAC TCCTCAAACG 
TGGATTTGGC TATGCTGGCT GGCTTCCCAG TTTTGGATCA CTATTCATTT ATGGAATCCA 
AAGCATGAAA GATTGGCAAA GAGTGAAAAA CTCTTCATTC TTCCCTACTA TATTGGCGCT 
TTTGTTGATC AATCCCTCGC ATTCAATCGC CGACGAGATG ATAAGGCAAA GATCAAGGCG 
GAAGATCTGG AATTCGACGC GGAAGACTCT TCACTCACCT ATGAAACGAT TCCAGGACTC 
CAGAATAAGA CTCCGCCAAG TGTTTGCTCG GCAAGTAGTT CAAAACTGGA AAATGGTTTG 
ATAAGGGATA GTGCAAGTTC TGCAGATGCT ATCACGAAGA TCTATGCCTG TGCGACTATG 
TGGCATGAGA CTGGTGTCGA GATGACGTGT ATGCTAAAAT CACTGTTTAG AATGGACGAG 
GATCAATGCG CTCGACGAAA TGCTCAAAAA TACCTAAAAG TTATCGATCC AGACTACTAC 
GAGTTTGAGG CCCACATCTT TTTCGATGAC GCTTACGATG TAAACGAGTA CGGGGAACCT 
GAAATCAACA AGTTCGTGAA ACAAATCGTT AATGTCATTG ATCAAGCCGC GTCAGCTGTC 
CATCAAACTC AGATGAGGCT GAAGCCACCC AAAAAGGCGA AAACTCCATA TGGTGGAAAG 
CTAACTTATA TTATGCCTGG AAAGAACAAA CTATTCATTC ATCTGAAGGA TAATCAAAAG 
ATCAGACACA GAAAGAGATG GAGTCAAGTG ATGTATTTGT ACTATCTACT TGGTTACAGA 
CTAATGATGA AGGTTGATGA TCCATCAAGA AAAGAAATTA TCTCTGAGAA TACGTTTATA 
TTGACTCTGG ACGGTGATGT TGACTTTACG CCGTCATCTG TCTATCTTCT TGTGGATCTT 
ATGAAGAAAA ATCGAAGACT CGGTGCGGCA TGCGGACGAA TTCATCCTAG AGGTGATGGT 
GCAATGGTGT GGTATCAGAA GTTTGAGTAC GCAATTGGAC ATTGGCTGCA AAAAGCGACA 
GAACATATGA TCGGTTGTGT TATGTGCTCG CCTGGATGCT TCTCGTTGTT TAGAGCATAT 
GCTCTGATGG ATGATAATGT CGCAAGGAGG TACGCTCTAA AATCCGAGGA GCCCAAGCAC 
TTTATCCAGT ACGATCAAGG TGAAGATCGT TGGTTATGCA CTTTACTTCT CCAACGAGGT 
TATCGAGTAG AATACTGCGC CGCTTCAGAC GCTCAGACCT TTGCCCCCGA AGGCTTCAAT 
GAGTTTTTCA ATCAACGCCG TCGCTGGATC CCATCAACAA TTTTCAATAT TATGGATTTA 
CTGAAGGACT ATCGAAATGT AGTAAGAGTC AACGAATCTA TCTCGATTTG GTATATCATA 
TATCAACTTG TCATGCTTAT CTCATCAATC CTCGGTCCTG GAACTATATT TGTCATGATC 
ATCGGAGCCA TCAGTATCTC ATTCTCAATT GATACTCTGA TTTCGTTGGT TATCGTGTCT 
ATTCCTGTTG TGGTGTTCAT TGTCGTCTGT CTTACTGCCA AGCCTGAACA TCAGCTAATT 
TGTGCACAGA CCATTGGTGC GATCTTTGCC ATGCTGATGA CAGCTGTCGT TGTAGGAACA 
TCCCTTCAGC TTCAGAAGGA CGGATTGCTA AGTCCTCACT CTATGTTCAC AGTGGCTGTC 
GCCACGTCGT TTTTGACTGC CGCAATACTT CATCCGCTCG AGTTCACCTG CATCATACCC 
GGAACCATTT ATTTCTTGGC GATTCCATGT ATGTATATGT TACTCCCGAT CTACAGTGTG 
TGCAACATGC ATACTGTATC CTGGGGTACT CGCGAAGACC CAAGGCCGAC GGAGAAAAAT 
ACTCTGGCTA AGAAAACTCC TGGAAACCTG GAATCTGGTG ATGGAGCCGG AAATTCTGAA 
AACTGGTGTA CCAGGTTCTT GTGCTGTGGT AGGGGTACGG TACACCCGAT GACTATGGTG 
ATCAATGAGA AGCTCAATGA AGTTATCAAG AAGGTTGACC GACTAGATCG AAAACATCAT 
CCATCGTTAG CTCGTCGTGC ATCAATTTTA TCAAGCACTG GTGGAACTAT TCAAATCGAC 
AAATGCTCGG AAGCCGATGA GGATGAGCAA GCGGAAATCG AAGACGCTCT GGAAATGTCA 
AATCAAAGTC ACGCGGCAAA GAAAAATCAA AAATGGAAGC AAGCACAAAG TGAAGCCTGG 
CTTGCGGACA AGGCGTTGAA GAGAGCAGAA CGAGAATATT TGGAACCAGA GGAGGAATCA 
TTTTGGAATG ATGTAATTGA ACGATATTTG TCTCCATTGA TTATGGACGG GAAGGATATG 
GATCGGCTGA GAGCTGGATT GATTGCTATC CGAAACTCCC ATACCGTATA CTTTTTAATG 
ATCAACATCG TATTCATCAT CTCTGTCCTA GTCCTACAAA TTCACAAAGA CTGTCTTAAC 
ATTGAATGGC CTTTGGGACC AAAATTCAAT CACACCGTAC GTCCATGTTA CGCAAATCAT 
GATGATAATC AGAAGGAAGA AGTTTGGGTT ATGACACGTC TTCAGCTGGA ACCCATCGGA 
TTAGTTTTCT TGATTTTCTT TGTTAGTATT CTGGTCATTC AGTTCCTTGC AATGTTGTGC 
CATCGGTTCG GAACCCTTGC TCATATCATC GCCTCCACAG AGCTTTTCTG TTTCAGAAAA 
ACAATGGACA GACTTAGCGA GGACGAGCTA GTTGCGCAAA ATGCTGTGGA AATTGCTCGA 
GAGTTGCAGG CGATACGGGG AATTGATGAA AACGCTCATA ATATTGATAA CCCAACCGAA 
GACCGTGGAA TATCACGCCG ACGTGTGGTA CAGAATCTTG AATCTTCGCG AAAATCAATG 
ATGAAAAGGA AGACAGAGAC CCTTGACGCA GCATTCAAGA AGCGATTCTT CGCCCTGTCA 
AGTGAACAAA CACCAGATCC GGCGGGATTT TCAGCCAGAG ACAATTCGAA ACGACTTACA 
TTGAGAAAAG GAACGATTCG AGCATTAGAG CACAGGAGGG ACTCACTGTT CGGAACTCTC 
GACAATCGAA AGGACGATGA AGTGGACGCA ACATCAATGC GTGGCCCTGC TCAACGCCGC 
TTGGAACGTC TTTTCACCGC ACAACAAGAC CAGAATAGTC CGACATCGGA CGGAAATCGA 
CGAAAATCGA ATAGCCGACC GTGGGATCAG CCAACATCTT CTGCCACGTC ATCAGGTGAC 
GTGGAGCTGA GAAGATTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300544.1
CDS	1..4821
Translation	MDRTMDRRDRDDNHSNYYIAEGTVTENKGWDIFRLLPPKPDRLGHGFWHDASLQVLKLATFLVLFLLTLGSAVVAKSTFILMTSAIGWGGQTITICNQVISEATQNTVKLKNAHVVKWVWATLLALSAPEALCFVRSMHRTMFRNVKRPTFIQFVFVLIIETFHSIGVGILVFRIFPDLDAVTAAQLTNAMCFVPAILSVISRKPNKSALLLVIIDFAAIAAQSSGFWALPMFLPNLQKHLVAIPVSLTLISLAWWQNFVHRDSVFPPVRTLAKFAQRLSERRSKTYAFVSLWKICIYVVCCFLFISSRMKIEDMLQKDPFGEKLLSVAGHDMNQTQIEKFQLRINQMIEQANREAGFYAAAEKKKQPPKKQPKADEAEQVDAGEYMMKRFKRFIGDAGENEEEEPEEEEFSSYNIYSNYVERNQLTMAYDALWLVIFQFGAVFVCYHSSKFACKVMMQRMGFALPMALSVPVTVLLLSTNCRMRQKDSCYGTNVLTVELFWQCNGASMSLADFILTPQTWIWLCWLASQFWITIHLWNPKHERLAKSEKLFILPYYIGAFVDQSLAFNRRRDDKAKIKAEDLEFDAEDSSLTYETIPGLQNKTPPSVCSASSSKLENGLIRDSASSADAITKIYACATMWHETGVEMTCMLKSLFRMDEDQCARRNAQKYLKVIDPDYYEFEAHIFFDDAYDVNEYGEPEINKFVKQIVNVIDQAASAVHQTQMRLKPPKKAKTPYGGKLTYIMPGKNKLFIHLKDNQKIRHRKRWSQVMYLYYLLGYRLMMKVDDPSRKEIISENTFILTLDGDVDFTPSSVYLLVDLMKKNRRLGAACGRIHPRGDGAMVWYQKFEYAIGHWLQKATEHMIGCVMCSPGCFSLFRAYALMDDNVARRYALKSEEPKHFIQYDQGEDRWLCTLLLQRGYRVEYCAASDAQTFAPEGFNEFFNQRRRWIPSTIFNIMDLLKDYRNVVRVNESISIWYIIYQLVMLISSILGPGTIFVMIIGAISISFSIDTLISLVIVSIPVVVFIVVCLTAKPEHQLICAQTIGAIFAMLMTAVVVGTSLQLQKDGLLSPHSMFTVAVATSFLTAAILHPLEFTCIIPGTIYFLAIPCMYMLLPIYSVCNMHTVSWGTREDPRPTEKNTLAKKTPGNLESGDGAGNSENWCTRFLCCGRGTVHPMTMVINEKLNEVIKKVDRLDRKHHPSLARRASILSSTGGTIQIDKCSEADEDEQAEIEDALEMSNQSHAAKKNQKWKQAQSEAWLADKALKRAEREYLEPEEESFWNDVIERYLSPLIMDGKDMDRLRAGLIAIRNSHTVYFLMINIVFIISVLVLQIHKDCLNIEWPLGPKFNHTVRPCYANHDDNQKEEVWVMTRLQLEPIGLVFLIFFVSILVIQFLAMLCHRFGTLAHIIASTELFCFRKTMDRLSEDELVAQNAVEIARELQAIRGIDENAHNIDNPTEDRGISRRRVVQNLESSRKSMMKRKTETLDAAFKKRFFALSSEQTPDPAGFSARDNSKRLTLRKGTIRALEHRRDSLFGTLDNRKDDEVDATSMRGPAQRRLERLFTAQQDQNSPTSDGNRRKSNSRPWDQPTSSATSSGDVELRRF

Target ORF information:

RefSeq Version	NM_001313615.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Chitin synthase chs-2 (chs-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313615.2

ORF Insert Sequence:

ATGGATCGAA CGATGGATCG TCGCGATCGC GATGACAATC ATTCAAATTA TTATATAGCT 
GAAGGAACAG TGACAGAAAA CAAAGGATGG GACATATTTC GACTGTTACC TCCAAAACCC 
GATCGACTTG GACATGGATT TTGGCACGAT GCAAGTCTTC AAGTTCTTAA ACTGGCCACT 
TTTTTGGTGC TCTTCCTGCT CACGCTCGGC TCAGCCGTTG TCGCCAAATC CACATTTATT 
CTAATGACGT CGGCAATTGG GTGGGGAGGA CAGACGATAA CAATTTGCAA TCAAGTAATA 
TCTGAAGCAA CTCAGAATAC TGTAAAGCTG AAAAATGCGC ATGTGGTGAA ATGGGTTTGG 
GCCACGTTAC TGGCACTGTC GGCTCCTGAA GCATTGTGCT TTGTGAGATC GATGCACCGG 
ACCATGTTCC GAAACGTGAA ACGCCCGACA TTCATTCAAT TTGTATTTGT GCTCATCATC 
GAAACATTTC ACAGCATTGG CGTCGGCATT CTCGTTTTCA GAATATTTCC AGACTTGGAT 
GCAGTGACAG CTGCACAGTT AACCAACGCG ATGTGTTTTG TACCAGCGAT TTTGTCAGTC 
ATCAGTCGAA AACCGAACAA GTCAGCGCTG TTGCTAGTAA TCATTGATTT TGCAGCGATT 
GCTGCACAAT CAAGCGGGTT TTGGGCTTTA CCGATGTTCC TGCCAAATCT TCAAAAGCAT 
TTGGTGGCTA TTCCAGTGTC GCTAACATTG ATTTCTCTTG CATGGTGGCA AAATTTTGTT 
CATCGAGATT CAGTCTTCCC ACCAGTGAGA ACATTAGCCA AATTTGCCCA ACGCTTGTCA 
GAACGTCGCT CAAAAACCTA CGCATTCGTT TCCCTTTGGA AAATCTGCAT CTACGTTGTC 
TGCTGCTTTC TTTTTATCTC AAGTCGAATG AAAATTGAAG ACATGCTCCA AAAAGACCCA 
TTCGGAGAAA AGTTGCTCTC TGTCGCAGGC CATGACATGA ATCAGACGCA AATCGAAAAG 
TTTCAATTGA GAATAAATCA GATGATTGAG CAAGCAAATC GCGAGGCCGG GTTCTACGCA 
GCAGCCGAAA AAAAGAAGCA GCCTCCCAAA AAACAACCGA AAGCTGATGA AGCTGAGCAA 
GTGGATGCTG GCGAATATAT GATGAAAAGG TTCAAGAGAT TCATTGGAGA CGCTGGAGAA 
AATGAGGAAG AGGAACCTGA AGAGGAAGAA TTTTCCAGTT ATAATATCTA CTCCAATTAC 
GTGGAACGTA ATCAGCTAAC AATGGCTTAT GATGCTTTAT GGCTTGTAAT ATTTCAATTT 
GGCGCTGTCT TTGTATGCTA CCACAGCTCA AAGTTTGCAT GTAAAGTAAT GATGCAACGA 
ATGGGATTTG CTCTTCCAAT GGCTCTCAGT GTACCTGTTA CTGTACTCTT GCTCTCCACA 
AATTGCCGAA TGAGACAGAA AGATTCTTGT TACGGTACAA ATGTGCTAAC TGTGGAGCTT 
TTCTGGCAAT GTAATGGTGC ATCAATGTCG CTTGCCGATT TCATTTTGAC TCCTCAAACG 
TGGATTTGGC TATGCTGGCT GGCTTCCCAG TTTTGGATCA CTATTCATTT ATGGAATCCA 
AAGCATGAAA GATTGGCAAA GAGTGAAAAA CTCTTCATTC TTCCCTACTA TATTGGCGCT 
TTTGTTGATC AATCCCTCGC ATTCAATCGC CGACGAGATG ATAAGGCAAA GATCAAGGCG 
GAAGATCTGG AATTCGACGC GGAAGACTCT TCACTCACCT ATGAAACGAT TCCAGGACTC 
CAGAATAAGA CTCCGCCAAG TGTTTGCTCG GCAAGTAGTT CAAAACTGGA AAATGGTTTG 
ATAAGGGATA GTGCAAGTTC TGCAGATGCT ATCACGAAGA TCTATGCCTG TGCGACTATG 
TGGCATGAGA CTGGTGTCGA GATGACGTGT ATGCTAAAAT CACTGTTTAG AATGGACGAG 
GATCAATGCG CTCGACGAAA TGCTCAAAAA TACCTAAAAG TTATCGATCC AGACTACTAC 
GAGTTTGAGG CCCACATCTT TTTCGATGAC GCTTACGATG TAAACGAGTA CGGGGAACCT 
GAAATCAACA AGTTCGTGAA ACAAATCGTT AATGTCATTG ATCAAGCCGC GTCAGCTGTC 
CATCAAACTC AGATGAGGCT GAAGCCACCC AAAAAGGCGA AAACTCCATA TGGTGGAAAG 
CTAACTTATA TTATGCCTGG AAAGAACAAA CTATTCATTC ATCTGAAGGA TAATCAAAAG 
ATCAGACACA GAAAGAGATG GAGTCAAGTG ATGTATTTGT ACTATCTACT TGGTTACAGA 
CTAATGATGA AGGTTGATGA TCCATCAAGA AAAGAAATTA TCTCTGAGAA TACGTTTATA 
TTGACTCTGG ACGGTGATGT TGACTTTACG CCGTCATCTG TCTATCTTCT TGTGGATCTT 
ATGAAGAAAA ATCGAAGACT CGGTGCGGCA TGCGGACGAA TTCATCCTAG AGGTGATGGT 
GCAATGGTGT GGTATCAGAA GTTTGAGTAC GCAATTGGAC ATTGGCTGCA AAAAGCGACA 
GAACATATGA TCGGTTGTGT TATGTGCTCG CCTGGATGCT TCTCGTTGTT TAGAGCATAT 
GCTCTGATGG ATGATAATGT CGCAAGGAGG TACGCTCTAA AATCCGAGGA GCCCAAGCAC 
TTTATCCAGT ACGATCAAGG TGAAGATCGT TGGTTATGCA CTTTACTTCT CCAACGAGGT 
TATCGAGTAG AATACTGCGC CGCTTCAGAC GCTCAGACCT TTGCCCCCGA AGGCTTCAAT 
GAGTTTTTCA ATCAACGCCG TCGCTGGATC CCATCAACAA TTTTCAATAT TATGGATTTA 
CTGAAGGACT ATCGAAATGT AGTAAGAGTC AACGAATCTA TCTCGATTTG GTATATCATA 
TATCAACTTG TCATGCTTAT CTCATCAATC CTCGGTCCTG GAACTATATT TGTCATGATC 
ATCGGAGCCA TCAGTATCTC ATTCTCAATT GATACTCTGA TTTCGTTGGT TATCGTGTCT 
ATTCCTGTTG TGGTGTTCAT TGTCGTCTGT CTTACTGCCA AGCCTGAACA TCAGCTAATT 
TGTGCACAGA CCATTGGTGC GATCTTTGCC ATGCTGATGA CAGCTGTCGT TGTAGGAACA 
TCCCTTCAGC TTCAGAAGGA CGGATTGCTA AGTCCTCACT CTATGTTCAC AGTGGCTGTC 
GCCACGTCGT TTTTGACTGC CGCAATACTT CATCCGCTCG AGTTCACCTG CATCATACCC 
GGAACCATTT ATTTCTTGGC GATTCCATGT ATGTATATGT TACTCCCGAT CTACAGTGTG 
TGCAACATGC ATACTGTATC CTGGGGTACT CGCGAAGACC CAAGGCCGAC GGAGAAAAAT 
ACTCTGGCTA AGAAAACTCC TGGAAACCTG GAATCTGGTG ATGGAGCCGG AAATTCTGAA 
AACTGGTGTA CCAGGTTCTT GTGCTGTGGT AGGGGTACGG TACACCCGAT GACTATGGTG 
ATCAATGAGA AGCTCAATGA AGTTATCAAG AAGGTTGACC GACTAGATCG AAAACATCAT 
CCATCGTTAG CTCGTCGTGC ATCAATTTTA TCAAGCACTG GTGGAACTAT TCAAATCGAC 
AAATGCTCGG AAGCCGATGA GGATGAGCAA GCGGAAATCG AAGACGCTCT GGAAATGTCA 
AATCAAAGTC ACGCGGCAAA GAAAAATCAA AAATGGAAGC AAGCACAAAG TGAAGCCTGG 
CTTGCGGACA AGGCGTTGAA GAGAGCAGAA CGAGAATATT TGGAACCAGA GGAGGAATCA 
TTTTGGAATG ATGTAATTGA ACGATATTTG TCTCCATTGA TTATGGACGG GAAGGATATG 
GATCGGCTGA GAGCTGGATT GATTGCTATC CGAAACTCCC ATACCGTATA CTTTTTAATG 
ATCAACATCG TATTCATCAT CTCTGTCCTA GTCCTACAAA TTCACAAAGA CTGTCTTAAC 
ATTGAATGGC CTTTGGGACC AAAATTCAAT CACACCGTAC GTCCATGTTA CGCAAATCAT 
GATGATAATC AGAAGGAAGA AGTTTGGGTT ATGACACGTC TTCAGCTGGA ACCCATCGGA 
TTAGTTTTCT TGATTTTCTT TGTTAGTATT CTGGTCATTC AGTTCCTTGC AATGTTGTGC 
CATCGGTTCG GAACCCTTGC TCATATCATC GCCTCCACAG AGCTTTTCTG TTTCAGAAAA 
ACAATGGACA GACTTAGCGA GGACGAGCTA GTTGCGCAAA ATGCTGTGGA AATTGCTCGA 
GAGTTGCAGG CGATACGGGG AATTGATGAA AACGCTCATA ATATTGATAA CCCAACCGAA 
GACCGTGGAA TATCACGCCG ACGTGTGGTA CAGAATCTTG AATCTTCGCG AAAATCAATG 
ATGAAAAGGA AGACAGAGAC CCTTGACGCA GCATTCAAGA AGCGATTCTT CGCCCTGTCA 
AGTGAACAAA CACCAGATCC GGCGGGATTT TCAGCCAGAG ACAATTCGAA ACGACTTACA 
TTGAGAAAAG GAACGATTCG AGCATTAGAG CACAGGAGGG ACTCACTGTT CGGAACTCTC 
GACAATCGAA AGGACGATGA AGTGGACGCA ACATCAATGC GTGGCCCTGC TCAACGCCGC 
TTGGAACGTC TTTTCACCGC ACAACAAGAC CAGAATAGTC CGACATCGGA CGGAAATCGA 
CGAAAATCGA ATAGCCGACC GTGGGATCAG CCAACATCTT CTGCCACGTC ATCAGGTGAC 
GTGGAGCTGA GAAGATTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chs-2 ( NM_001313616.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300545.1 Caenorhabditis elegans Chitin synthase chs-2 (chs-2), partial mRNA.

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CloneID	OCa339543
Clone ID Related Accession (Same CDS sequence)	NM_001313616.2 , NM_001313616.1
Accession Version	NM_001313616.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Chitin synthase chs-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_001313616.1. COMPLETENESS: incomplete on the 5' end.

ATGGACAGTG GTAATAAGGA AAAGGAGTTT GTGGAGGAGT GGGTTCAGGA CTCACATACT 
GGAGCCCAGG TCCCTCTCGA TCATTTGGTA TCCCTCTACG GATATGCGCT TCTTGGCCTT 
GAAGATTCTT CGGAGACGGT TAGATTTCGA AAAATTGAAA CCCAAATTGA GGAATCTGGA 
GTCAAGGATG AAGAAGAGCA ACACTATTCC AGTTCCCAAG AGGTAGCAAA GAAGGAAGAG 
GATAAAGAAT ATGAAGAGCC AAAAAGGAGA GTCAGGACCG AAATGGATCG AACGATGGAT 
CGTCGCGATC GCGATGACAA TCATTCAAAT TATTATATAG CTGAAGGAAC AGTGACAGAA 
AACAAAGGAT GGGACATATT TCGACTGTTA CCTCCAAAAC CCGATCGACT TGGACATGGA 
TTTTGGCACG ATGCAAGTCT TCAAGTTCTT AAACTGGCCA CTTTTTTGGT GCTCTTCCTG 
CTCACGCTCG GCTCAGCCGT TGTCGCCAAA TCCACATTTA TTCTAATGAC GTCGGCAATT 
GGGTGGGGAG GACAGACGAT AACAATTTGC AATCAAGTAA TATCTGAAGC AACTCAGAAT 
ACTGTAAAGC TGAAAAATGC GCATGTGGTG AAATGGGTTT GGGCCACGTT ACTGGCACTG 
TCGGCTCCTG AAGCATTGTG CTTTGTGAGA TCGATGCACC GGACCATGTT CCGAAACGTG 
AAACGCCCGA CATTCATTCA ATTTGTATTT GTGCTCATCA TCGAAACATT TCACAGCATT 
GGCGTCGGCA TTCTCGTTTT CAGAATATTT CCAGACTTGG ATGCAGTGAC AGCTGCACAG 
TTAACCAACG CGATGTGTTT TGTACCAGCG ATTTTGTCAG TCATCAGTCG AAAACCGAAC 
AAGTCAGCGC TGTTGCTAGT AATCATTGAT TTTGCAGCGA TTGCTGCACA ATCAAGCGGG 
TTTTGGGCTT TACCGATGTT CCTGCCAAAT CTTCAAAAGC ATTTGGTGGC TATTCCAGTG 
TCGCTAACAT TGATTTCTCT TGCATGGTGG CAAAATTTTG TTCATCGAGA TTCAGTCTTC 
CCACCAGTGA GAACATTAGC CAAATTTGCC CAACGCTTGT CAGAACGTCG CTCAAAAACC 
TACGCATTCG TTTCCCTTTG GAAAATCTGC ATCTACGTTG TCTGCTGCTT TCTTTTTATC 
TCAAGTCGAA TGAAAATTGA AGACATGCTC CAAAAAGACC CATTCGGAGA AAAGTTGCTC 
TCTGTCGCAG GCCATGACAT GAATCAGACG CAAATCGAAA AGTTTCAATT GAGAATAAAT 
CAGATGATTG AGCAAGCAAA TCGCGAGGCC GGGTTCTACG CAGCAGCCGA AAAAAAGAAG 
CAGCCTCCCA AAAAACAACC GAAAGCTGAT GAAGCTGAGC AAGTGGATGC TGGCGAATAT 
ATGATGAAAA GGTTCAAGAG ATTCATTGGA GACGCTGGAG AAAATGAGGA AGAGGAACCT 
GAAGAGGAAG AATTTTCCAG TTATAATATC TACTCCAATT ACGTGGAACG TAATCAGCTA 
ACAATGGCTT ATGATGCTTT ATGGCTTGTA ATATTTCAAT TTGGCGCTGT CTTTGTATGC 
TACCACAGCT CAAAGTTTGC ATGTAAAGTA ATGATGCAAC GAATGGGATT TGCTCTTCCA 
ATGGCTCTCA GTGTACCTGT TACTGTACTC TTGCTCTCCA CAAATTGCCG AATGAGACAG 
AAAGATTCTT GTTACGGTAC AAATGTGCTA ACTGTGGAGC TTTTCTGGCA ATGTAATGGT 
GCATCAATGT CGCTTGCCGA TTTCATTTTG ACTCCTCAAA CGTGGATTTG GCTATGCTGG 
CTGGCTTCCC AGTTTTGGAT CACTATTCAT TTATGGAATC CAAAGCATGA AAGATTGGCA 
AAGAGTGAAA AACTCTTCAT TCTTCCCTAC TATATTGGCG CTTTTGTTGA TCAATCCCTC 
GCATTCAATC GCCGACGAGA TGATAAGGCA AAGATCAAGG CGGAAGATCT GGAATTCGAC 
GCGGAAGACT CTTCACTCAC CTATGAAACG ATTCCAGGAC TCCAGAATAA GACTCCGCCA 
AGTGTTTGCT CGGCAAGTAG TTCAAAACTG GAAAATGGTT TGATAAGGGA TAGTGCAAGT 
TCTGCAGATG CTATCACGAA GATCTATGCC TGTGCGACTA TGTGGCATGA GACTGGTGTC 
GAGATGACGT GTATGCTAAA ATCACTGTTT AGAATGGACG AGGATCAATG CGCTCGACGA 
AATGCTCAAA AATACCTAAA AGTTATCGAT CCAGACTACT ACGAGTTTGA GGCCCACATC 
TTTTTCGATG ACGCTTACGA TGTAAACGAG TACGGGGAAC CTGAAATCAA CAAGTTCGTG 
AAACAAATCG TTAATGTCAT TGATCAAGCC GCGTCAGCTG TCCATCAAAC TCAGATGAGG 
CTGAAGCCAC CCAAAAAGGC GAAAACTCCA TATGGTGGAA AGCTAACTTA TATTATGCCT 
GGAAAGAACA AACTATTCAT TCATCTGAAG GATAATCAAA AGATCAGACA CAGAAAGAGA 
TGGAGTCAAG TGATGTATTT GTACTATCTA CTTGGTTACA GACTAATGAT GAAGGTTGAT 
GATCCATCAA GAAAAGAAAT TATCTCTGAG AATACGTTTA TATTGACTCT GGACGGTGAT 
GTTGACTTTA CGCCGTCATC TGTCTATCTT CTTGTGGATC TTATGAAGAA AAATCGAAGA 
CTCGGTGCGG CATGCGGACG AATTCATCCT AGAGGTGATG GTGCAATGGT GTGGTATCAG 
AAGTTTGAGT ACGCAATTGG ACATTGGCTG CAAAAAGCGA CAGAACATAT GATCGGTTGT 
GTTATGTGCT CGCCTGGATG CTTCTCGTTG TTTAGAGCAT ATGCTCTGAT GGATGATAAT 
GTCGCAAGGA GGTACGCTCT AAAATCCGAG GAGCCCAAGC ACTTTATCCA GTACGATCAA 
GGTGAAGATC GTTGGTTATG CACTTTACTT CTCCAACGAG GTTATCGAGT AGAATACTGC 
GCCGCTTCAG ACGCTCAGAC CTTTGCCCCC GAAGGCTTCA ATGAGTTTTT CAATCAACGC 
CGTCGCTGGA TCCCATCAAC AATTTTCAAT ATTATGGATT TACTGAAGGA CTATCGAAAT 
GTAGTAAGAG TCAACGAATC TATCTCGATT TGGTATATCA TATATCAACT TGTCATGCTT 
ATCTCATCAA TCCTCGGTCC TGGAACTATA TTTGTCATGA TCATCGGAGC CATCAGTATC 
TCATTCTCAA TTGATACTCT GATTTCGTTG GTTATCGTGT CTATTCCTGT TGTGGTGTTC 
ATTGTCGTCT GTCTTACTGC CAAGCCTGAA CATCAGCTAA TTTGTGCACA GACCATTGGT 
GCGATCTTTG CCATGCTGAT GACAGCTGTC GTTGTAGGAA CATCCCTTCA GCTTCAGAAG 
GACGGATTGC TAAGTCCTCA CTCTATGTTC ACAGTGGCTG TCGCCACGTC GTTTTTGACT 
GCCGCAATAC TTCATCCGCT CGAGTTCACC TGCATCATAC CCGGAACCAT TTATTTCTTG 
GCGATTCCAT GTATGTATAT GTTACTCCCG ATCTACAGTG TGTGCAACAT GCATACTGTA 
TCCTGGGGTA CTCGCGAAGA CCCAAGGCCG ACGGAGAAAA ATACTCTGGC TAAGAAAACT 
CCTGGAAACC TGGAATCTGG TGATGGAGCC GGAAATTCTG AAAACTGGTG TACCAGGTTC 
TTGTGCTGTG GTAGGGGTAC GGTACACCCG ATGACTATGG TGATCAATGA GAAGCTCAAT 
GAAGTTATCA AGAAGGTTGA CCGACTAGAT CGAAAACATC ATCCATCGTT AGCTCGTCGT 
GCATCAATTT TATCAAGCAC TGGTGGAACT ATTCAAATCG ACAAATGCTC GGAAGCCGAT 
GAGGATGAGC AAGCGGAAAT CGAAGACGCT CTGGAAATGT CAAATCAAAG TCACGCGGCA 
AAGAAAAATC AAAAATGGAA GCAAGCACAA AGTGAAGCCT GGCTTGCGGA CAAGGCGTTG 
AAGAGAGCAG AACGAGAATA TTTGGAACCA GAGGAGGAAT CATTTTGGAA TGATGTAATT 
GAACGATATT TGTCTCCATT GATTATGGAC GGGAAGGATA TGGATCGGCT GAGAGCTGGA 
TTGATTGCTA TCCGAAACTC CCATACCGTA TACTTTTTAA TGATCAACAT CGTATTCATC 
ATCTCTGTCC TAGTCCTACA AATTCACAAA GACTGTCTTA ACATTGAATG GCCTTTGGGA 
CCAAAATTCA ATCACACCGT ACGTCCATGT TACGCAAATC ATGATGATAA TCAGAAGGAA 
GAAGTTTGGG TTATGACACG TCTTCAGCTG GAACCCATCG GATTAGTTTT CTTGATTTTC 
TTTGTTAGTA TTCTGGTCAT TCAGTTCCTT GCAATGTTGT GCCATCGGTT CGGAACCCTT 
GCTCATATCA TCGCCTCCAC AGAGCTTTTC TGTTTCAGAA AAACAATGGA CAGACTTAGC 
GAGGACGAGC TAGTTGCGCA AAATGCTGTG GAAATTGCTC GAGAGTTGCA GGCGATACGG 
GGAATTGATG AAAACGCTCA TAATATTGAT AACCCAACCG AAGACCGTGG AATATCACGC 
CGACGTGTGG TACAGAATCT TGAATCTTCG CGAAAATCAA TGATGAAAAG GAAGACAGAG 
ACCCTTGACG CAGCATTCAA GAAGCGATTC TTCGCCCTGT CAAGTGAACA AACACCAGAT 
CCGGCGGGAT TTTCAGCCAG AGACAATTCG AAACGACTTA CATTGAGAAA AGGAACGATT 
CGAGCATTAG AGCACAGGAG GGACTCACTG TTCGGAACTC TCGACAATCG AAAGGACGAT 
GAAGTGGACG CAACATCAAT GCGTGGCCCT GCTCAACGCC GCTTGGAACG TCTTTTCACC 
GCACAACAAG ACCAGAATAG TCCGACATCG GACGGAAATC GACGAAAATC GAATAGCCGA 
CCGTGGGATC AGCCAACATC TTCTGCCACG TCATCAGGTG ACGTGGAGCT GAGAAGATTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300545.1
CDS	1..5103
Translation	MDSGNKEKEFVEEWVQDSHTGAQVPLDHLVSLYGYALLGLEDSSETVRFRKIETQIEESGVKDEEEQHYSSSQEVAKKEEDKEYEEPKRRVRTEMDRTMDRRDRDDNHSNYYIAEGTVTENKGWDIFRLLPPKPDRLGHGFWHDASLQVLKLATFLVLFLLTLGSAVVAKSTFILMTSAIGWGGQTITICNQVISEATQNTVKLKNAHVVKWVWATLLALSAPEALCFVRSMHRTMFRNVKRPTFIQFVFVLIIETFHSIGVGILVFRIFPDLDAVTAAQLTNAMCFVPAILSVISRKPNKSALLLVIIDFAAIAAQSSGFWALPMFLPNLQKHLVAIPVSLTLISLAWWQNFVHRDSVFPPVRTLAKFAQRLSERRSKTYAFVSLWKICIYVVCCFLFISSRMKIEDMLQKDPFGEKLLSVAGHDMNQTQIEKFQLRINQMIEQANREAGFYAAAEKKKQPPKKQPKADEAEQVDAGEYMMKRFKRFIGDAGENEEEEPEEEEFSSYNIYSNYVERNQLTMAYDALWLVIFQFGAVFVCYHSSKFACKVMMQRMGFALPMALSVPVTVLLLSTNCRMRQKDSCYGTNVLTVELFWQCNGASMSLADFILTPQTWIWLCWLASQFWITIHLWNPKHERLAKSEKLFILPYYIGAFVDQSLAFNRRRDDKAKIKAEDLEFDAEDSSLTYETIPGLQNKTPPSVCSASSSKLENGLIRDSASSADAITKIYACATMWHETGVEMTCMLKSLFRMDEDQCARRNAQKYLKVIDPDYYEFEAHIFFDDAYDVNEYGEPEINKFVKQIVNVIDQAASAVHQTQMRLKPPKKAKTPYGGKLTYIMPGKNKLFIHLKDNQKIRHRKRWSQVMYLYYLLGYRLMMKVDDPSRKEIISENTFILTLDGDVDFTPSSVYLLVDLMKKNRRLGAACGRIHPRGDGAMVWYQKFEYAIGHWLQKATEHMIGCVMCSPGCFSLFRAYALMDDNVARRYALKSEEPKHFIQYDQGEDRWLCTLLLQRGYRVEYCAASDAQTFAPEGFNEFFNQRRRWIPSTIFNIMDLLKDYRNVVRVNESISIWYIIYQLVMLISSILGPGTIFVMIIGAISISFSIDTLISLVIVSIPVVVFIVVCLTAKPEHQLICAQTIGAIFAMLMTAVVVGTSLQLQKDGLLSPHSMFTVAVATSFLTAAILHPLEFTCIIPGTIYFLAIPCMYMLLPIYSVCNMHTVSWGTREDPRPTEKNTLAKKTPGNLESGDGAGNSENWCTRFLCCGRGTVHPMTMVINEKLNEVIKKVDRLDRKHHPSLARRASILSSTGGTIQIDKCSEADEDEQAEIEDALEMSNQSHAAKKNQKWKQAQSEAWLADKALKRAEREYLEPEEESFWNDVIERYLSPLIMDGKDMDRLRAGLIAIRNSHTVYFLMINIVFIISVLVLQIHKDCLNIEWPLGPKFNHTVRPCYANHDDNQKEEVWVMTRLQLEPIGLVFLIFFVSILVIQFLAMLCHRFGTLAHIIASTELFCFRKTMDRLSEDELVAQNAVEIARELQAIRGIDENAHNIDNPTEDRGISRRRVVQNLESSRKSMMKRKTETLDAAFKKRFFALSSEQTPDPAGFSARDNSKRLTLRKGTIRALEHRRDSLFGTLDNRKDDEVDATSMRGPAQRRLERLFTAQQDQNSPTSDGNRRKSNSRPWDQPTSSATSSGDVELRRF

Target ORF information:

RefSeq Version	NM_001313616.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Chitin synthase chs-2 (chs-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313616.2

ORF Insert Sequence:

ATGGACAGTG GTAATAAGGA AAAGGAGTTT GTGGAGGAGT GGGTTCAGGA CTCACATACT 
GGAGCCCAGG TCCCTCTCGA TCATTTGGTA TCCCTCTACG GATATGCGCT TCTTGGCCTT 
GAAGATTCTT CGGAGACGGT TAGATTTCGA AAAATTGAAA CCCAAATTGA GGAATCTGGA 
GTCAAGGATG AAGAAGAGCA ACACTATTCC AGTTCCCAAG AGGTAGCAAA GAAGGAAGAG 
GATAAAGAAT ATGAAGAGCC AAAAAGGAGA GTCAGGACCG AAATGGATCG AACGATGGAT 
CGTCGCGATC GCGATGACAA TCATTCAAAT TATTATATAG CTGAAGGAAC AGTGACAGAA 
AACAAAGGAT GGGACATATT TCGACTGTTA CCTCCAAAAC CCGATCGACT TGGACATGGA 
TTTTGGCACG ATGCAAGTCT TCAAGTTCTT AAACTGGCCA CTTTTTTGGT GCTCTTCCTG 
CTCACGCTCG GCTCAGCCGT TGTCGCCAAA TCCACATTTA TTCTAATGAC GTCGGCAATT 
GGGTGGGGAG GACAGACGAT AACAATTTGC AATCAAGTAA TATCTGAAGC AACTCAGAAT 
ACTGTAAAGC TGAAAAATGC GCATGTGGTG AAATGGGTTT GGGCCACGTT ACTGGCACTG 
TCGGCTCCTG AAGCATTGTG CTTTGTGAGA TCGATGCACC GGACCATGTT CCGAAACGTG 
AAACGCCCGA CATTCATTCA ATTTGTATTT GTGCTCATCA TCGAAACATT TCACAGCATT 
GGCGTCGGCA TTCTCGTTTT CAGAATATTT CCAGACTTGG ATGCAGTGAC AGCTGCACAG 
TTAACCAACG CGATGTGTTT TGTACCAGCG ATTTTGTCAG TCATCAGTCG AAAACCGAAC 
AAGTCAGCGC TGTTGCTAGT AATCATTGAT TTTGCAGCGA TTGCTGCACA ATCAAGCGGG 
TTTTGGGCTT TACCGATGTT CCTGCCAAAT CTTCAAAAGC ATTTGGTGGC TATTCCAGTG 
TCGCTAACAT TGATTTCTCT TGCATGGTGG CAAAATTTTG TTCATCGAGA TTCAGTCTTC 
CCACCAGTGA GAACATTAGC CAAATTTGCC CAACGCTTGT CAGAACGTCG CTCAAAAACC 
TACGCATTCG TTTCCCTTTG GAAAATCTGC ATCTACGTTG TCTGCTGCTT TCTTTTTATC 
TCAAGTCGAA TGAAAATTGA AGACATGCTC CAAAAAGACC CATTCGGAGA AAAGTTGCTC 
TCTGTCGCAG GCCATGACAT GAATCAGACG CAAATCGAAA AGTTTCAATT GAGAATAAAT 
CAGATGATTG AGCAAGCAAA TCGCGAGGCC GGGTTCTACG CAGCAGCCGA AAAAAAGAAG 
CAGCCTCCCA AAAAACAACC GAAAGCTGAT GAAGCTGAGC AAGTGGATGC TGGCGAATAT 
ATGATGAAAA GGTTCAAGAG ATTCATTGGA GACGCTGGAG AAAATGAGGA AGAGGAACCT 
GAAGAGGAAG AATTTTCCAG TTATAATATC TACTCCAATT ACGTGGAACG TAATCAGCTA 
ACAATGGCTT ATGATGCTTT ATGGCTTGTA ATATTTCAAT TTGGCGCTGT CTTTGTATGC 
TACCACAGCT CAAAGTTTGC ATGTAAAGTA ATGATGCAAC GAATGGGATT TGCTCTTCCA 
ATGGCTCTCA GTGTACCTGT TACTGTACTC TTGCTCTCCA CAAATTGCCG AATGAGACAG 
AAAGATTCTT GTTACGGTAC AAATGTGCTA ACTGTGGAGC TTTTCTGGCA ATGTAATGGT 
GCATCAATGT CGCTTGCCGA TTTCATTTTG ACTCCTCAAA CGTGGATTTG GCTATGCTGG 
CTGGCTTCCC AGTTTTGGAT CACTATTCAT TTATGGAATC CAAAGCATGA AAGATTGGCA 
AAGAGTGAAA AACTCTTCAT TCTTCCCTAC TATATTGGCG CTTTTGTTGA TCAATCCCTC 
GCATTCAATC GCCGACGAGA TGATAAGGCA AAGATCAAGG CGGAAGATCT GGAATTCGAC 
GCGGAAGACT CTTCACTCAC CTATGAAACG ATTCCAGGAC TCCAGAATAA GACTCCGCCA 
AGTGTTTGCT CGGCAAGTAG TTCAAAACTG GAAAATGGTT TGATAAGGGA TAGTGCAAGT 
TCTGCAGATG CTATCACGAA GATCTATGCC TGTGCGACTA TGTGGCATGA GACTGGTGTC 
GAGATGACGT GTATGCTAAA ATCACTGTTT AGAATGGACG AGGATCAATG CGCTCGACGA 
AATGCTCAAA AATACCTAAA AGTTATCGAT CCAGACTACT ACGAGTTTGA GGCCCACATC 
TTTTTCGATG ACGCTTACGA TGTAAACGAG TACGGGGAAC CTGAAATCAA CAAGTTCGTG 
AAACAAATCG TTAATGTCAT TGATCAAGCC GCGTCAGCTG TCCATCAAAC TCAGATGAGG 
CTGAAGCCAC CCAAAAAGGC GAAAACTCCA TATGGTGGAA AGCTAACTTA TATTATGCCT 
GGAAAGAACA AACTATTCAT TCATCTGAAG GATAATCAAA AGATCAGACA CAGAAAGAGA 
TGGAGTCAAG TGATGTATTT GTACTATCTA CTTGGTTACA GACTAATGAT GAAGGTTGAT 
GATCCATCAA GAAAAGAAAT TATCTCTGAG AATACGTTTA TATTGACTCT GGACGGTGAT 
GTTGACTTTA CGCCGTCATC TGTCTATCTT CTTGTGGATC TTATGAAGAA AAATCGAAGA 
CTCGGTGCGG CATGCGGACG AATTCATCCT AGAGGTGATG GTGCAATGGT GTGGTATCAG 
AAGTTTGAGT ACGCAATTGG ACATTGGCTG CAAAAAGCGA CAGAACATAT GATCGGTTGT 
GTTATGTGCT CGCCTGGATG CTTCTCGTTG TTTAGAGCAT ATGCTCTGAT GGATGATAAT 
GTCGCAAGGA GGTACGCTCT AAAATCCGAG GAGCCCAAGC ACTTTATCCA GTACGATCAA 
GGTGAAGATC GTTGGTTATG CACTTTACTT CTCCAACGAG GTTATCGAGT AGAATACTGC 
GCCGCTTCAG ACGCTCAGAC CTTTGCCCCC GAAGGCTTCA ATGAGTTTTT CAATCAACGC 
CGTCGCTGGA TCCCATCAAC AATTTTCAAT ATTATGGATT TACTGAAGGA CTATCGAAAT 
GTAGTAAGAG TCAACGAATC TATCTCGATT TGGTATATCA TATATCAACT TGTCATGCTT 
ATCTCATCAA TCCTCGGTCC TGGAACTATA TTTGTCATGA TCATCGGAGC CATCAGTATC 
TCATTCTCAA TTGATACTCT GATTTCGTTG GTTATCGTGT CTATTCCTGT TGTGGTGTTC 
ATTGTCGTCT GTCTTACTGC CAAGCCTGAA CATCAGCTAA TTTGTGCACA GACCATTGGT 
GCGATCTTTG CCATGCTGAT GACAGCTGTC GTTGTAGGAA CATCCCTTCA GCTTCAGAAG 
GACGGATTGC TAAGTCCTCA CTCTATGTTC ACAGTGGCTG TCGCCACGTC GTTTTTGACT 
GCCGCAATAC TTCATCCGCT CGAGTTCACC TGCATCATAC CCGGAACCAT TTATTTCTTG 
GCGATTCCAT GTATGTATAT GTTACTCCCG ATCTACAGTG TGTGCAACAT GCATACTGTA 
TCCTGGGGTA CTCGCGAAGA CCCAAGGCCG ACGGAGAAAA ATACTCTGGC TAAGAAAACT 
CCTGGAAACC TGGAATCTGG TGATGGAGCC GGAAATTCTG AAAACTGGTG TACCAGGTTC 
TTGTGCTGTG GTAGGGGTAC GGTACACCCG ATGACTATGG TGATCAATGA GAAGCTCAAT 
GAAGTTATCA AGAAGGTTGA CCGACTAGAT CGAAAACATC ATCCATCGTT AGCTCGTCGT 
GCATCAATTT TATCAAGCAC TGGTGGAACT ATTCAAATCG ACAAATGCTC GGAAGCCGAT 
GAGGATGAGC AAGCGGAAAT CGAAGACGCT CTGGAAATGT CAAATCAAAG TCACGCGGCA 
AAGAAAAATC AAAAATGGAA GCAAGCACAA AGTGAAGCCT GGCTTGCGGA CAAGGCGTTG 
AAGAGAGCAG AACGAGAATA TTTGGAACCA GAGGAGGAAT CATTTTGGAA TGATGTAATT 
GAACGATATT TGTCTCCATT GATTATGGAC GGGAAGGATA TGGATCGGCT GAGAGCTGGA 
TTGATTGCTA TCCGAAACTC CCATACCGTA TACTTTTTAA TGATCAACAT CGTATTCATC 
ATCTCTGTCC TAGTCCTACA AATTCACAAA GACTGTCTTA ACATTGAATG GCCTTTGGGA 
CCAAAATTCA ATCACACCGT ACGTCCATGT TACGCAAATC ATGATGATAA TCAGAAGGAA 
GAAGTTTGGG TTATGACACG TCTTCAGCTG GAACCCATCG GATTAGTTTT CTTGATTTTC 
TTTGTTAGTA TTCTGGTCAT TCAGTTCCTT GCAATGTTGT GCCATCGGTT CGGAACCCTT 
GCTCATATCA TCGCCTCCAC AGAGCTTTTC TGTTTCAGAA AAACAATGGA CAGACTTAGC 
GAGGACGAGC TAGTTGCGCA AAATGCTGTG GAAATTGCTC GAGAGTTGCA GGCGATACGG 
GGAATTGATG AAAACGCTCA TAATATTGAT AACCCAACCG AAGACCGTGG AATATCACGC 
CGACGTGTGG TACAGAATCT TGAATCTTCG CGAAAATCAA TGATGAAAAG GAAGACAGAG 
ACCCTTGACG CAGCATTCAA GAAGCGATTC TTCGCCCTGT CAAGTGAACA AACACCAGAT 
CCGGCGGGAT TTTCAGCCAG AGACAATTCG AAACGACTTA CATTGAGAAA AGGAACGATT 
CGAGCATTAG AGCACAGGAG GGACTCACTG TTCGGAACTC TCGACAATCG AAAGGACGAT 
GAAGTGGACG CAACATCAAT GCGTGGCCCT GCTCAACGCC GCTTGGAACG TCTTTTCACC 
GCACAACAAG ACCAGAATAG TCCGACATCG GACGGAAATC GACGAAAATC GAATAGCCGA 
CCGTGGGATC AGCCAACATC TTCTGCCACG TCATCAGGTG ACGTGGAGCT GAGAAGATTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chs-2 ( NM_001313616.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001300545.1 Caenorhabditis elegans Chitin synthase chs-2 (chs-2), partial mRNA.

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CloneID	OCa339543
Clone ID Related Accession (Same CDS sequence)	NM_001313616.2 , NM_001313616.1
Accession Version	NM_001313616.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Chitin synthase chs-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGGACAGTG GTAATAAGGA AAAGGAGTTT GTGGAGGAGT GGGTTCAGGA CTCACATACT 
GGAGCCCAGG TCCCTCTCGA TCATTTGGTA TCCCTCTACG GATATGCGCT TCTTGGCCTT 
GAAGATTCTT CGGAGACGGT TAGATTTCGA AAAATTGAAA CCCAAATTGA GGAATCTGGA 
GTCAAGGATG AAGAAGAGCA ACACTATTCC AGTTCCCAAG AGGTAGCAAA GAAGGAAGAG 
GATAAAGAAT ATGAAGAGCC AAAAAGGAGA GTCAGGACCG AAATGGATCG AACGATGGAT 
CGTCGCGATC GCGATGACAA TCATTCAAAT TATTATATAG CTGAAGGAAC AGTGACAGAA 
AACAAAGGAT GGGACATATT TCGACTGTTA CCTCCAAAAC CCGATCGACT TGGACATGGA 
TTTTGGCACG ATGCAAGTCT TCAAGTTCTT AAACTGGCCA CTTTTTTGGT GCTCTTCCTG 
CTCACGCTCG GCTCAGCCGT TGTCGCCAAA TCCACATTTA TTCTAATGAC GTCGGCAATT 
GGGTGGGGAG GACAGACGAT AACAATTTGC AATCAAGTAA TATCTGAAGC AACTCAGAAT 
ACTGTAAAGC TGAAAAATGC GCATGTGGTG AAATGGGTTT GGGCCACGTT ACTGGCACTG 
TCGGCTCCTG AAGCATTGTG CTTTGTGAGA TCGATGCACC GGACCATGTT CCGAAACGTG 
AAACGCCCGA CATTCATTCA ATTTGTATTT GTGCTCATCA TCGAAACATT TCACAGCATT 
GGCGTCGGCA TTCTCGTTTT CAGAATATTT CCAGACTTGG ATGCAGTGAC AGCTGCACAG 
TTAACCAACG CGATGTGTTT TGTACCAGCG ATTTTGTCAG TCATCAGTCG AAAACCGAAC 
AAGTCAGCGC TGTTGCTAGT AATCATTGAT TTTGCAGCGA TTGCTGCACA ATCAAGCGGG 
TTTTGGGCTT TACCGATGTT CCTGCCAAAT CTTCAAAAGC ATTTGGTGGC TATTCCAGTG 
TCGCTAACAT TGATTTCTCT TGCATGGTGG CAAAATTTTG TTCATCGAGA TTCAGTCTTC 
CCACCAGTGA GAACATTAGC CAAATTTGCC CAACGCTTGT CAGAACGTCG CTCAAAAACC 
TACGCATTCG TTTCCCTTTG GAAAATCTGC ATCTACGTTG TCTGCTGCTT TCTTTTTATC 
TCAAGTCGAA TGAAAATTGA AGACATGCTC CAAAAAGACC CATTCGGAGA AAAGTTGCTC 
TCTGTCGCAG GCCATGACAT GAATCAGACG CAAATCGAAA AGTTTCAATT GAGAATAAAT 
CAGATGATTG AGCAAGCAAA TCGCGAGGCC GGGTTCTACG CAGCAGCCGA AAAAAAGAAG 
CAGCCTCCCA AAAAACAACC GAAAGCTGAT GAAGCTGAGC AAGTGGATGC TGGCGAATAT 
ATGATGAAAA GGTTCAAGAG ATTCATTGGA GACGCTGGAG AAAATGAGGA AGAGGAACCT 
GAAGAGGAAG AATTTTCCAG TTATAATATC TACTCCAATT ACGTGGAACG TAATCAGCTA 
ACAATGGCTT ATGATGCTTT ATGGCTTGTA ATATTTCAAT TTGGCGCTGT CTTTGTATGC 
TACCACAGCT CAAAGTTTGC ATGTAAAGTA ATGATGCAAC GAATGGGATT TGCTCTTCCA 
ATGGCTCTCA GTGTACCTGT TACTGTACTC TTGCTCTCCA CAAATTGCCG AATGAGACAG 
AAAGATTCTT GTTACGGTAC AAATGTGCTA ACTGTGGAGC TTTTCTGGCA ATGTAATGGT 
GCATCAATGT CGCTTGCCGA TTTCATTTTG ACTCCTCAAA CGTGGATTTG GCTATGCTGG 
CTGGCTTCCC AGTTTTGGAT CACTATTCAT TTATGGAATC CAAAGCATGA AAGATTGGCA 
AAGAGTGAAA AACTCTTCAT TCTTCCCTAC TATATTGGCG CTTTTGTTGA TCAATCCCTC 
GCATTCAATC GCCGACGAGA TGATAAGGCA AAGATCAAGG CGGAAGATCT GGAATTCGAC 
GCGGAAGACT CTTCACTCAC CTATGAAACG ATTCCAGGAC TCCAGAATAA GACTCCGCCA 
AGTGTTTGCT CGGCAAGTAG TTCAAAACTG GAAAATGGTT TGATAAGGGA TAGTGCAAGT 
TCTGCAGATG CTATCACGAA GATCTATGCC TGTGCGACTA TGTGGCATGA GACTGGTGTC 
GAGATGACGT GTATGCTAAA ATCACTGTTT AGAATGGACG AGGATCAATG CGCTCGACGA 
AATGCTCAAA AATACCTAAA AGTTATCGAT CCAGACTACT ACGAGTTTGA GGCCCACATC 
TTTTTCGATG ACGCTTACGA TGTAAACGAG TACGGGGAAC CTGAAATCAA CAAGTTCGTG 
AAACAAATCG TTAATGTCAT TGATCAAGCC GCGTCAGCTG TCCATCAAAC TCAGATGAGG 
CTGAAGCCAC CCAAAAAGGC GAAAACTCCA TATGGTGGAA AGCTAACTTA TATTATGCCT 
GGAAAGAACA AACTATTCAT TCATCTGAAG GATAATCAAA AGATCAGACA CAGAAAGAGA 
TGGAGTCAAG TGATGTATTT GTACTATCTA CTTGGTTACA GACTAATGAT GAAGGTTGAT 
GATCCATCAA GAAAAGAAAT TATCTCTGAG AATACGTTTA TATTGACTCT GGACGGTGAT 
GTTGACTTTA CGCCGTCATC TGTCTATCTT CTTGTGGATC TTATGAAGAA AAATCGAAGA 
CTCGGTGCGG CATGCGGACG AATTCATCCT AGAGGTGATG GTGCAATGGT GTGGTATCAG 
AAGTTTGAGT ACGCAATTGG ACATTGGCTG CAAAAAGCGA CAGAACATAT GATCGGTTGT 
GTTATGTGCT CGCCTGGATG CTTCTCGTTG TTTAGAGCAT ATGCTCTGAT GGATGATAAT 
GTCGCAAGGA GGTACGCTCT AAAATCCGAG GAGCCCAAGC ACTTTATCCA GTACGATCAA 
GGTGAAGATC GTTGGTTATG CACTTTACTT CTCCAACGAG GTTATCGAGT AGAATACTGC 
GCCGCTTCAG ACGCTCAGAC CTTTGCCCCC GAAGGCTTCA ATGAGTTTTT CAATCAACGC 
CGTCGCTGGA TCCCATCAAC AATTTTCAAT ATTATGGATT TACTGAAGGA CTATCGAAAT 
GTAGTAAGAG TCAACGAATC TATCTCGATT TGGTATATCA TATATCAACT TGTCATGCTT 
ATCTCATCAA TCCTCGGTCC TGGAACTATA TTTGTCATGA TCATCGGAGC CATCAGTATC 
TCATTCTCAA TTGATACTCT GATTTCGTTG GTTATCGTGT CTATTCCTGT TGTGGTGTTC 
ATTGTCGTCT GTCTTACTGC CAAGCCTGAA CATCAGCTAA TTTGTGCACA GACCATTGGT 
GCGATCTTTG CCATGCTGAT GACAGCTGTC GTTGTAGGAA CATCCCTTCA GCTTCAGAAG 
GACGGATTGC TAAGTCCTCA CTCTATGTTC ACAGTGGCTG TCGCCACGTC GTTTTTGACT 
GCCGCAATAC TTCATCCGCT CGAGTTCACC TGCATCATAC CCGGAACCAT TTATTTCTTG 
GCGATTCCAT GTATGTATAT GTTACTCCCG ATCTACAGTG TGTGCAACAT GCATACTGTA 
TCCTGGGGTA CTCGCGAAGA CCCAAGGCCG ACGGAGAAAA ATACTCTGGC TAAGAAAACT 
CCTGGAAACC TGGAATCTGG TGATGGAGCC GGAAATTCTG AAAACTGGTG TACCAGGTTC 
TTGTGCTGTG GTAGGGGTAC GGTACACCCG ATGACTATGG TGATCAATGA GAAGCTCAAT 
GAAGTTATCA AGAAGGTTGA CCGACTAGAT CGAAAACATC ATCCATCGTT AGCTCGTCGT 
GCATCAATTT TATCAAGCAC TGGTGGAACT ATTCAAATCG ACAAATGCTC GGAAGCCGAT 
GAGGATGAGC AAGCGGAAAT CGAAGACGCT CTGGAAATGT CAAATCAAAG TCACGCGGCA 
AAGAAAAATC AAAAATGGAA GCAAGCACAA AGTGAAGCCT GGCTTGCGGA CAAGGCGTTG 
AAGAGAGCAG AACGAGAATA TTTGGAACCA GAGGAGGAAT CATTTTGGAA TGATGTAATT 
GAACGATATT TGTCTCCATT GATTATGGAC GGGAAGGATA TGGATCGGCT GAGAGCTGGA 
TTGATTGCTA TCCGAAACTC CCATACCGTA TACTTTTTAA TGATCAACAT CGTATTCATC 
ATCTCTGTCC TAGTCCTACA AATTCACAAA GACTGTCTTA ACATTGAATG GCCTTTGGGA 
CCAAAATTCA ATCACACCGT ACGTCCATGT TACGCAAATC ATGATGATAA TCAGAAGGAA 
GAAGTTTGGG TTATGACACG TCTTCAGCTG GAACCCATCG GATTAGTTTT CTTGATTTTC 
TTTGTTAGTA TTCTGGTCAT TCAGTTCCTT GCAATGTTGT GCCATCGGTT CGGAACCCTT 
GCTCATATCA TCGCCTCCAC AGAGCTTTTC TGTTTCAGAA AAACAATGGA CAGACTTAGC 
GAGGACGAGC TAGTTGCGCA AAATGCTGTG GAAATTGCTC GAGAGTTGCA GGCGATACGG 
GGAATTGATG AAAACGCTCA TAATATTGAT AACCCAACCG AAGACCGTGG AATATCACGC 
CGACGTGTGG TACAGAATCT TGAATCTTCG CGAAAATCAA TGATGAAAAG GAAGACAGAG 
ACCCTTGACG CAGCATTCAA GAAGCGATTC TTCGCCCTGT CAAGTGAACA AACACCAGAT 
CCGGCGGGAT TTTCAGCCAG AGACAATTCG AAACGACTTA CATTGAGAAA AGGAACGATT 
CGAGCATTAG AGCACAGGAG GGACTCACTG TTCGGAACTC TCGACAATCG AAAGGACGAT 
GAAGTGGACG CAACATCAAT GCGTGGCCCT GCTCAACGCC GCTTGGAACG TCTTTTCACC 
GCACAACAAG ACCAGAATAG TCCGACATCG GACGGAAATC GACGAAAATC GAATAGCCGA 
CCGTGGGATC AGCCAACATC TTCTGCCACG TCATCAGGTG ACGTGGAGCT GAGAAGATTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300545.1
CDS	1..5103
Translation	MDSGNKEKEFVEEWVQDSHTGAQVPLDHLVSLYGYALLGLEDSSETVRFRKIETQIEESGVKDEEEQHYSSSQEVAKKEEDKEYEEPKRRVRTEMDRTMDRRDRDDNHSNYYIAEGTVTENKGWDIFRLLPPKPDRLGHGFWHDASLQVLKLATFLVLFLLTLGSAVVAKSTFILMTSAIGWGGQTITICNQVISEATQNTVKLKNAHVVKWVWATLLALSAPEALCFVRSMHRTMFRNVKRPTFIQFVFVLIIETFHSIGVGILVFRIFPDLDAVTAAQLTNAMCFVPAILSVISRKPNKSALLLVIIDFAAIAAQSSGFWALPMFLPNLQKHLVAIPVSLTLISLAWWQNFVHRDSVFPPVRTLAKFAQRLSERRSKTYAFVSLWKICIYVVCCFLFISSRMKIEDMLQKDPFGEKLLSVAGHDMNQTQIEKFQLRINQMIEQANREAGFYAAAEKKKQPPKKQPKADEAEQVDAGEYMMKRFKRFIGDAGENEEEEPEEEEFSSYNIYSNYVERNQLTMAYDALWLVIFQFGAVFVCYHSSKFACKVMMQRMGFALPMALSVPVTVLLLSTNCRMRQKDSCYGTNVLTVELFWQCNGASMSLADFILTPQTWIWLCWLASQFWITIHLWNPKHERLAKSEKLFILPYYIGAFVDQSLAFNRRRDDKAKIKAEDLEFDAEDSSLTYETIPGLQNKTPPSVCSASSSKLENGLIRDSASSADAITKIYACATMWHETGVEMTCMLKSLFRMDEDQCARRNAQKYLKVIDPDYYEFEAHIFFDDAYDVNEYGEPEINKFVKQIVNVIDQAASAVHQTQMRLKPPKKAKTPYGGKLTYIMPGKNKLFIHLKDNQKIRHRKRWSQVMYLYYLLGYRLMMKVDDPSRKEIISENTFILTLDGDVDFTPSSVYLLVDLMKKNRRLGAACGRIHPRGDGAMVWYQKFEYAIGHWLQKATEHMIGCVMCSPGCFSLFRAYALMDDNVARRYALKSEEPKHFIQYDQGEDRWLCTLLLQRGYRVEYCAASDAQTFAPEGFNEFFNQRRRWIPSTIFNIMDLLKDYRNVVRVNESISIWYIIYQLVMLISSILGPGTIFVMIIGAISISFSIDTLISLVIVSIPVVVFIVVCLTAKPEHQLICAQTIGAIFAMLMTAVVVGTSLQLQKDGLLSPHSMFTVAVATSFLTAAILHPLEFTCIIPGTIYFLAIPCMYMLLPIYSVCNMHTVSWGTREDPRPTEKNTLAKKTPGNLESGDGAGNSENWCTRFLCCGRGTVHPMTMVINEKLNEVIKKVDRLDRKHHPSLARRASILSSTGGTIQIDKCSEADEDEQAEIEDALEMSNQSHAAKKNQKWKQAQSEAWLADKALKRAEREYLEPEEESFWNDVIERYLSPLIMDGKDMDRLRAGLIAIRNSHTVYFLMINIVFIISVLVLQIHKDCLNIEWPLGPKFNHTVRPCYANHDDNQKEEVWVMTRLQLEPIGLVFLIFFVSILVIQFLAMLCHRFGTLAHIIASTELFCFRKTMDRLSEDELVAQNAVEIARELQAIRGIDENAHNIDNPTEDRGISRRRVVQNLESSRKSMMKRKTETLDAAFKKRFFALSSEQTPDPAGFSARDNSKRLTLRKGTIRALEHRRDSLFGTLDNRKDDEVDATSMRGPAQRRLERLFTAQQDQNSPTSDGNRRKSNSRPWDQPTSSATSSGDVELRRF

Target ORF information:

RefSeq Version	NM_001313616.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Chitin synthase chs-2 (chs-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313616.1

ORF Insert Sequence:

ATGGACAGTG GTAATAAGGA AAAGGAGTTT GTGGAGGAGT GGGTTCAGGA CTCACATACT 
GGAGCCCAGG TCCCTCTCGA TCATTTGGTA TCCCTCTACG GATATGCGCT TCTTGGCCTT 
GAAGATTCTT CGGAGACGGT TAGATTTCGA AAAATTGAAA CCCAAATTGA GGAATCTGGA 
GTCAAGGATG AAGAAGAGCA ACACTATTCC AGTTCCCAAG AGGTAGCAAA GAAGGAAGAG 
GATAAAGAAT ATGAAGAGCC AAAAAGGAGA GTCAGGACCG AAATGGATCG AACGATGGAT 
CGTCGCGATC GCGATGACAA TCATTCAAAT TATTATATAG CTGAAGGAAC AGTGACAGAA 
AACAAAGGAT GGGACATATT TCGACTGTTA CCTCCAAAAC CCGATCGACT TGGACATGGA 
TTTTGGCACG ATGCAAGTCT TCAAGTTCTT AAACTGGCCA CTTTTTTGGT GCTCTTCCTG 
CTCACGCTCG GCTCAGCCGT TGTCGCCAAA TCCACATTTA TTCTAATGAC GTCGGCAATT 
GGGTGGGGAG GACAGACGAT AACAATTTGC AATCAAGTAA TATCTGAAGC AACTCAGAAT 
ACTGTAAAGC TGAAAAATGC GCATGTGGTG AAATGGGTTT GGGCCACGTT ACTGGCACTG 
TCGGCTCCTG AAGCATTGTG CTTTGTGAGA TCGATGCACC GGACCATGTT CCGAAACGTG 
AAACGCCCGA CATTCATTCA ATTTGTATTT GTGCTCATCA TCGAAACATT TCACAGCATT 
GGCGTCGGCA TTCTCGTTTT CAGAATATTT CCAGACTTGG ATGCAGTGAC AGCTGCACAG 
TTAACCAACG CGATGTGTTT TGTACCAGCG ATTTTGTCAG TCATCAGTCG AAAACCGAAC 
AAGTCAGCGC TGTTGCTAGT AATCATTGAT TTTGCAGCGA TTGCTGCACA ATCAAGCGGG 
TTTTGGGCTT TACCGATGTT CCTGCCAAAT CTTCAAAAGC ATTTGGTGGC TATTCCAGTG 
TCGCTAACAT TGATTTCTCT TGCATGGTGG CAAAATTTTG TTCATCGAGA TTCAGTCTTC 
CCACCAGTGA GAACATTAGC CAAATTTGCC CAACGCTTGT CAGAACGTCG CTCAAAAACC 
TACGCATTCG TTTCCCTTTG GAAAATCTGC ATCTACGTTG TCTGCTGCTT TCTTTTTATC 
TCAAGTCGAA TGAAAATTGA AGACATGCTC CAAAAAGACC CATTCGGAGA AAAGTTGCTC 
TCTGTCGCAG GCCATGACAT GAATCAGACG CAAATCGAAA AGTTTCAATT GAGAATAAAT 
CAGATGATTG AGCAAGCAAA TCGCGAGGCC GGGTTCTACG CAGCAGCCGA AAAAAAGAAG 
CAGCCTCCCA AAAAACAACC GAAAGCTGAT GAAGCTGAGC AAGTGGATGC TGGCGAATAT 
ATGATGAAAA GGTTCAAGAG ATTCATTGGA GACGCTGGAG AAAATGAGGA AGAGGAACCT 
GAAGAGGAAG AATTTTCCAG TTATAATATC TACTCCAATT ACGTGGAACG TAATCAGCTA 
ACAATGGCTT ATGATGCTTT ATGGCTTGTA ATATTTCAAT TTGGCGCTGT CTTTGTATGC 
TACCACAGCT CAAAGTTTGC ATGTAAAGTA ATGATGCAAC GAATGGGATT TGCTCTTCCA 
ATGGCTCTCA GTGTACCTGT TACTGTACTC TTGCTCTCCA CAAATTGCCG AATGAGACAG 
AAAGATTCTT GTTACGGTAC AAATGTGCTA ACTGTGGAGC TTTTCTGGCA ATGTAATGGT 
GCATCAATGT CGCTTGCCGA TTTCATTTTG ACTCCTCAAA CGTGGATTTG GCTATGCTGG 
CTGGCTTCCC AGTTTTGGAT CACTATTCAT TTATGGAATC CAAAGCATGA AAGATTGGCA 
AAGAGTGAAA AACTCTTCAT TCTTCCCTAC TATATTGGCG CTTTTGTTGA TCAATCCCTC 
GCATTCAATC GCCGACGAGA TGATAAGGCA AAGATCAAGG CGGAAGATCT GGAATTCGAC 
GCGGAAGACT CTTCACTCAC CTATGAAACG ATTCCAGGAC TCCAGAATAA GACTCCGCCA 
AGTGTTTGCT CGGCAAGTAG TTCAAAACTG GAAAATGGTT TGATAAGGGA TAGTGCAAGT 
TCTGCAGATG CTATCACGAA GATCTATGCC TGTGCGACTA TGTGGCATGA GACTGGTGTC 
GAGATGACGT GTATGCTAAA ATCACTGTTT AGAATGGACG AGGATCAATG CGCTCGACGA 
AATGCTCAAA AATACCTAAA AGTTATCGAT CCAGACTACT ACGAGTTTGA GGCCCACATC 
TTTTTCGATG ACGCTTACGA TGTAAACGAG TACGGGGAAC CTGAAATCAA CAAGTTCGTG 
AAACAAATCG TTAATGTCAT TGATCAAGCC GCGTCAGCTG TCCATCAAAC TCAGATGAGG 
CTGAAGCCAC CCAAAAAGGC GAAAACTCCA TATGGTGGAA AGCTAACTTA TATTATGCCT 
GGAAAGAACA AACTATTCAT TCATCTGAAG GATAATCAAA AGATCAGACA CAGAAAGAGA 
TGGAGTCAAG TGATGTATTT GTACTATCTA CTTGGTTACA GACTAATGAT GAAGGTTGAT 
GATCCATCAA GAAAAGAAAT TATCTCTGAG AATACGTTTA TATTGACTCT GGACGGTGAT 
GTTGACTTTA CGCCGTCATC TGTCTATCTT CTTGTGGATC TTATGAAGAA AAATCGAAGA 
CTCGGTGCGG CATGCGGACG AATTCATCCT AGAGGTGATG GTGCAATGGT GTGGTATCAG 
AAGTTTGAGT ACGCAATTGG ACATTGGCTG CAAAAAGCGA CAGAACATAT GATCGGTTGT 
GTTATGTGCT CGCCTGGATG CTTCTCGTTG TTTAGAGCAT ATGCTCTGAT GGATGATAAT 
GTCGCAAGGA GGTACGCTCT AAAATCCGAG GAGCCCAAGC ACTTTATCCA GTACGATCAA 
GGTGAAGATC GTTGGTTATG CACTTTACTT CTCCAACGAG GTTATCGAGT AGAATACTGC 
GCCGCTTCAG ACGCTCAGAC CTTTGCCCCC GAAGGCTTCA ATGAGTTTTT CAATCAACGC 
CGTCGCTGGA TCCCATCAAC AATTTTCAAT ATTATGGATT TACTGAAGGA CTATCGAAAT 
GTAGTAAGAG TCAACGAATC TATCTCGATT TGGTATATCA TATATCAACT TGTCATGCTT 
ATCTCATCAA TCCTCGGTCC TGGAACTATA TTTGTCATGA TCATCGGAGC CATCAGTATC 
TCATTCTCAA TTGATACTCT GATTTCGTTG GTTATCGTGT CTATTCCTGT TGTGGTGTTC 
ATTGTCGTCT GTCTTACTGC CAAGCCTGAA CATCAGCTAA TTTGTGCACA GACCATTGGT 
GCGATCTTTG CCATGCTGAT GACAGCTGTC GTTGTAGGAA CATCCCTTCA GCTTCAGAAG 
GACGGATTGC TAAGTCCTCA CTCTATGTTC ACAGTGGCTG TCGCCACGTC GTTTTTGACT 
GCCGCAATAC TTCATCCGCT CGAGTTCACC TGCATCATAC CCGGAACCAT TTATTTCTTG 
GCGATTCCAT GTATGTATAT GTTACTCCCG ATCTACAGTG TGTGCAACAT GCATACTGTA 
TCCTGGGGTA CTCGCGAAGA CCCAAGGCCG ACGGAGAAAA ATACTCTGGC TAAGAAAACT 
CCTGGAAACC TGGAATCTGG TGATGGAGCC GGAAATTCTG AAAACTGGTG TACCAGGTTC 
TTGTGCTGTG GTAGGGGTAC GGTACACCCG ATGACTATGG TGATCAATGA GAAGCTCAAT 
GAAGTTATCA AGAAGGTTGA CCGACTAGAT CGAAAACATC ATCCATCGTT AGCTCGTCGT 
GCATCAATTT TATCAAGCAC TGGTGGAACT ATTCAAATCG ACAAATGCTC GGAAGCCGAT 
GAGGATGAGC AAGCGGAAAT CGAAGACGCT CTGGAAATGT CAAATCAAAG TCACGCGGCA 
AAGAAAAATC AAAAATGGAA GCAAGCACAA AGTGAAGCCT GGCTTGCGGA CAAGGCGTTG 
AAGAGAGCAG AACGAGAATA TTTGGAACCA GAGGAGGAAT CATTTTGGAA TGATGTAATT 
GAACGATATT TGTCTCCATT GATTATGGAC GGGAAGGATA TGGATCGGCT GAGAGCTGGA 
TTGATTGCTA TCCGAAACTC CCATACCGTA TACTTTTTAA TGATCAACAT CGTATTCATC 
ATCTCTGTCC TAGTCCTACA AATTCACAAA GACTGTCTTA ACATTGAATG GCCTTTGGGA 
CCAAAATTCA ATCACACCGT ACGTCCATGT TACGCAAATC ATGATGATAA TCAGAAGGAA 
GAAGTTTGGG TTATGACACG TCTTCAGCTG GAACCCATCG GATTAGTTTT CTTGATTTTC 
TTTGTTAGTA TTCTGGTCAT TCAGTTCCTT GCAATGTTGT GCCATCGGTT CGGAACCCTT 
GCTCATATCA TCGCCTCCAC AGAGCTTTTC TGTTTCAGAA AAACAATGGA CAGACTTAGC 
GAGGACGAGC TAGTTGCGCA AAATGCTGTG GAAATTGCTC GAGAGTTGCA GGCGATACGG 
GGAATTGATG AAAACGCTCA TAATATTGAT AACCCAACCG AAGACCGTGG AATATCACGC 
CGACGTGTGG TACAGAATCT TGAATCTTCG CGAAAATCAA TGATGAAAAG GAAGACAGAG 
ACCCTTGACG CAGCATTCAA GAAGCGATTC TTCGCCCTGT CAAGTGAACA AACACCAGAT 
CCGGCGGGAT TTTCAGCCAG AGACAATTCG AAACGACTTA CATTGAGAAA AGGAACGATT 
CGAGCATTAG AGCACAGGAG GGACTCACTG TTCGGAACTC TCGACAATCG AAAGGACGAT 
GAAGTGGACG CAACATCAAT GCGTGGCCCT GCTCAACGCC GCTTGGAACG TCTTTTCACC 
GCACAACAAG ACCAGAATAG TCCGACATCG GACGGAAATC GACGAAAATC GAATAGCCGA 
CCGTGGGATC AGCCAACATC TTCTGCCACG TCATCAGGTG ACGTGGAGCT GAGAAGATTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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オンライン注文

chs-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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