lrk-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	lrk-1
Entrez Gene ID	172995
Full Name	Leucine-rich repeat serine/threonine-protein kinase 1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_060438.6	NP_492839.4	Leucine-rich repeat serine/threonine-protein kinase 1
NM_060438.7	NP_492839.4	Leucine-rich repeat serine/threonine-protein kinase 1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000810	XP_311349.5
Drosophila melanogaster (fruit fly)	Lrrk	NP_001097847.1
Caenorhabditis elegans (roundworm)	lrk-1	NP_492839.4

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study investigated the role of UNC-23 and HSP-1 on LRK-1 function in C. elegans; results suggest that the UNC-23-HSP-1 chaperone complex regulates LRK-1 localization to the Golgi apparatus and consequently determines the polarized sorting of synaptic vesicle proteins to the axons.
Title: Chaperone complex BAG2-HSC70 regulates localization of Caenorhabditis elegans leucine-rich repeat kinase LRK-1 to the Golgi.

Lack of Grx1 homolog exacerbates LRRK2-mediated dopaminergic neurodegeneration in Caenorhabditis elegans.
Title: Glutaredoxin deficiency exacerbates neurodegeneration in C. elegans models of Parkinson's disease.

Findings establish a critical functional link between LRRK2 and ER stress.
Title: Dysregulated LRRK2 signaling in response to endoplasmic reticulum stress leads to dopaminergic neuron degeneration in C. elegans.

Due to the similarity of the C. elegans proteins to human LRRK2 and PINK1, we suggest a common role of both factors in cellular functions including stress response and regulation of neurite outgrowth
Title: Caenorhabditits elegans LRK-1 and PINK-1 act antagonistically in stress response and neurite outgrowth.

LRRK2 can regulate the mitochondrial physiology of Caenorhabditis elegans, possibly via the worm lrk-1 pathway.
Title: Investigating convergent actions of genes linked to familial Parkinson's disease.

The following lrk-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lrk-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa337000	NM_060438.7 Latest version!	Caenorhabditis elegans Leucine-rich repeat serine/threonine-protein kinase 1 (lrk-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
OCa337000	NM_060438.6	Caenorhabditis elegans Leucine-rich repeat serine/threonine-protein kinase 1 (lrk-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lrk-1 ( NM_060438.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492839.4 Caenorhabditis elegans Leucine-rich repeat serine/threonine-protein kinase 1 (lrk-1), partial mRNA.

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CloneID	OCa337000
Clone ID Related Accession (Same CDS sequence)	NM_060438.6 , NM_060438.7
Accession Version	NM_060438.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Leucine-rich repeat serine/threonine-protein kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_060438.5. COMPLETENESS: incomplete on both ends.

ATGGACCTCT CAAGTGGGGG TCCGTCATCT TCAAGCGATG TAGCTTCAGA ATTGGATAAT 
TCGGATGCAA TGCAACTGGT TAGACAGGCA GTATTATTCG AGAACGTGGA GCTGCTGGCC 
GACTTGTTCA AAGTGAATCC TTGGGTATGG AATCGGGTTG ATCGACATGG AAGGACACCG 
TTAATGTTAG CTGCTCATAA TGGGAAGCTG GATAGTTTGA GGACTATTTT AATGCTTAGT 
CCTAATTCTC TAAATCTTGT GAACGATCGT GGTAAGACCG CACTTCATAT GGCAGCCGAA 
TCAGGTGAAA CTTCGATTGT TTTGGAGCTT GTCGAACTGG GCTCCGATCC AATGAAATCT 
GATAATGAAG GACATTGTGC ATTGGAGTTA GCTCAAATGG CTGGACATAA TGAGGTTGCC 
GCAAAGCTTA TTGATGCTAT TCAAAAAGAA AGTGAAGATC TAAATGAAGC CCACACAATG 
ATCATCAGTG CATGCATATC TGGAAGTGCT GACGTTGTTT ATGAAATTTC AAGGAGATTT 
ATGGAAAAAA AACAAAGCCG CGAAATATTA TTCAACGGTC GAAATGAAGA GGATGAGACT 
GCACTCTTGA TTGCCTGCAC AAACGGTCAT ATCGAAATCG TCCGTCACTT ATTACAATTC 
GAAGAGCATC TTCTACAAAG CCACGTATCC AAGGATACAG TAATCCATGC GGCAGTGTCG 
TCACAGAATG TGGAAGTGTT GCAGCTGTGT TTGGAGAAAT TCCCTCAACT CGTCAAATCT 
ACCAACAACG AGGGCTCTAC GTGTCTACAT TGGGCCGCCC GTTGTGGATC TTCGGAATGT 
GTTTCCACAA TTCTAAACTT TCCATTCCCA TCTGAATTTA TAATCGAAAT CGACACAGTT 
GGAGCACCAG CTTATCAGCT TGCTCTAGAC GTGAATGAAG TTGACGGAGA GTGTCGAACG 
GCAATGTATC TAGCGGTTGC CGAAGGACAT TTGGAAGTTG TCAAGGCGAT GACTGATTTC 
AAATGCACAT CAATCGACGG AAGACAACGT TGCCCGTTTC AACTTGACGT CTACTGTACT 
CGAGGACGAA CACCATTCAT GTTGGCCGCT TTTAATCAGA ATCTTCCATT GATGACGCTT 
CTTCTCGACG CTGGTGCAGA TGTCAACTTG CCGCTGGCAG TCTTGGACAC AGAGTACAGC 
GTAGAAGAAG GTCGATGCAT TGGCTCGGGT GCTCTCGTTG AAGCTGTACG ATCTGATGGA 
CTACACATTG TGCACTTTCT GTTAGACCGT GGAGCTCTGG ACACTGATAA CAAGGCTCTT 
CGACTTGCTG CTCAGGGGAA GAACGAGAAG CTCATTCGGG TTTTTCTGGT TCGCCTGGTT 
TTTGCGGACC CTGAATATAA GATCAATAAG AAAAACATTG ATGTAGGTCA GATTCAAGTT 
GGTCAGAGTC TGCTACCAAG TTCACTGTGC CCATCAAAAG CCGCCCAACT GAATTGGAAT 
TCAGCCAATT TGGAGCAACT TCAATCGGAT TGGTTTGTGG CGGCAGCTCT ACACGTGAAC 
CCGAGACTCC GAACAACGAG GTTATCACTG GCAGCAATCA CTCGGGTCGA TTTATCGGAC 
AATCGTCTCA ACACATTCCC AAGTATTCTA TTCCAAATGC CATCGCTCAG ATCTCTAAAT 
CTAGCTGATA ATAGTATACG TAAAATTGAA ATTCCAACTT ATTACATTAG TTCAACATCG 
CTAGAGATTC TCAATTTACG GAATAATCAA CTCGAGTGTA TAGCCATTCA ATTTCTATCA 
TCTCTTCCAC AATTGCAACA ATTGGATGTA TCGAAGAACG AGTTGAGCCA ACTTCCCGAG 
TATATATGGT TGTGTCCAGC GTTGAAAGAG CTCAATGCAT CCTATAATAG ACTGAGCACA 
CTTCCGATGG TTGCAAGAGC ATCAAGAGGA GAGAGACCAC GACTCAATAA TTCTAACAAC 
AATTTCAACA CCCAATCTCC AACACAAGAA TCGAATCCAA TCGTCGTTGA TGATCCTCCG 
AATGTCACTT CAAACCCTCT GCGTCGCCAA AATGTGTGGC AAGCGAGTAT CAATTTGTCA 
AAAGTCGACG ACGATTCCCT GTTCCCCGAC TTCCCGGTCA CTTCCAGTAA CACATTGACG 
ACGATAAATC TATCTTTCAA TAAATTCCAC ACATTCCCAT TCTGCCTGGC ATGCACATGT 
CCCCGGCTTC TGATTCTAAA CATGTCAAAC AACTCAATGA CGTCACTTCC ACCTATGGCT 
TGTGTACCTG CGCATCTGAG GACGTTGGAT CTGTCTTACA ATAAGATACA AGAAAGTTTC 
ATCGAAGCTT CACCTCTCCA CGTGGTCTGT CACGCGGTCC CACCGACAAC GTCTAATGGC 
TCAATGCTCC CAAAGCGAAG AAATTCTCCA GCTCGGCAAC ATAGAAGTCG TTCGAAGTCT 
GCAGTTAGAT CACAGAGAAG TCTCAGTGTT TCTCGCCATC ATGCGCTAAT TGATCCACAA 
AAAGAAGAAG AAAGTTGTGT TCATAAGCGT CACGATTCAT TAGAATGGCT CAAGACACTA 
CAATTGGCTG GAAATCGACT CCGATCAATT TCAGTGACAA ATGCAGCTTC AAAAGTTCTC 
CTTCCAGCTC TGAACGTGAT GGATATCAGT GACAACAAAT TGCTACAAGC ACCACCGGAT 
GTTGCACGGC TCACACTTCT TTCAATGCTC AACTTATCAG GAAATACTGC AATTAAAGAA 
CTACCACCAG ATTATGGAAT GCTCTCTCGT CTTTGGAGTC TATCTTTGAA AGGATGCTCA 
CTGAAAGAAC CACTTGAATC AATGGTTAAT GTGGAGAACT GCAAGACAGT GGAAATTGTG 
GCGTATTTGA AGACAATTCT AGAAGAATCC AAGACATACC ATCATTTGAG ATTAATGATT 
TTGGGAAGTG ATGGCGTTGG AAAATCTGTT ATTTGGGATG CACTGTGCAA GGAAGCAGTA 
CAGAAACGGC AGCCTATTCA CTCGGAAACT GGTGTTATTC GGCAAGCTGA ATGGAAGTTC 
GAAGCGAAAC GATCTAAAGG CGATAAGAAT CTTGGGCCTG TCGGATTTTC TGTAATCGAC 
TTTGGAGGTC AACGAGAATA TCATTCCACT CATCAATATT TCCTGAGCAA ACGATCCTTG 
AACTTGGTTC TTTGGAAGAT CACAGACGGG GATGAAGCAC TGGCGCAGCT TGATACTTGG 
CTAGTTAATA TCCATGCAAG AGCGCCAAAC TCGACAGTAA TACTAGTGGG AACCAATTTG 
GATCAAGTGG CATCGAATAG TTCGAAATTT GGACCTGGAT ACATAGACAT CATGGAGCAA 
AAAGTGCGGA CCCGTTATAT GGTTGCAGAT GCGGATAAGT CAGGATTGCC ACGTATTGTT 
GATGTAATTT TGATCAATTC AACGTCAAGA AATGATGTAA AAGCACTGCT AAACACAATA 
TATAGAACTG CGTGGGAAGT ACGAATGGGA AAAGAAAGAG CTATGGAACA ACAGATTCCA 
TCTTCTTACA TTGCTTTGAT GAAAGTAACA AAAGAATTAG GCGTCGAGTT TCGGAAAGAG 
GGGCAGCCGG CTGTAATGAC TGTAGAGGCA TATAGAGAAA GAGTCAAGAA AAGGATGATT 
AGCAAGTTTG GCAGACCGTT TAGGGACGAC ATTGAATTCT ACGCCGCATG CACATTCCTT 
CACGATTGTG GTGAATTAGT TCGATTTGAG GATGCCACTC TTCGGGATCT GATTTTTGTA 
GATCCGCTGT GGCTCGCCGA GTTTCTCACT TCTGTCGTCA TTCTAAGAAG CCCAAATCTT 
CCAGCTGGAC TTCTTTCAAC AGACGCTATA AATCCGCATA CTCGTTCATT CAAATCAGGA 
GCACTTTTAA TGCTCAAAAC ACAGCTTTTG GATCTTCTTC ACAAATTCGA ATTGGCACTT 
GCTACCCAGC CTCGACAGCT TCTGATTCCA AGCCTGCTAC CAGATGAGTA CAGGCTACGA 
TCTGATTTCT TAGCATCTGC AGTTAAAATC CGAATGAAAA TGTCACAGTG GAATGTCAGA 
TGTCCAAGTC CCGCTGGATC ACCGACCAAA TCCCCACTCC GTCGAACATC ACCTACGGAT 
CAGAACGGTG TTGGATCAGA GGATGTTATG CTTCAATTCA CTTATGACGA TGATCAATTG 
TTAAGAAGGA TTTATGCGTT GGCTTATATA CCTTCAGGAT TTTGGAGTCG CCTAGTGACT 
CGTATAGTTG GAGATAAAAA CGTGTGTGCA GCAATTGAAA GTATTTTCAT GACAACAAGT 
GCGGATCGAG CAAAGATTGC AGATATAGCC ACTAAGCATG CAAAAGCTGA ATGGGTTGTG 
TGGCAAACTG GAATAGAGCT TCATGTTAAA GGACATTCAC TTTTCACTCT GAAACAGTTT 
CTACCGTTGG CAGAGGTTAG AGACATTGAC TACAGCGCCA TAGACATGCG CGCAAAGGAT 
GAACAAAAAC GATGGAGGAC ATGGAATCAA CCTTCACACC GCCCAATTGT CGAAATGGTT 
GTTAACTCAT TGAGCATTTC AGCAGCTTCA CAACACGGAA GAAAACTTTC AATGAAAACA 
GATGTGGAAG GAAGAAGCCG ATTATTGGCA ATGATATCAG ATTTATTGGA CACGTTACTC 
GAAGACTGGT ATCCGGCTCT TGGAACTCGA TTTGTTCACA GTTCGGAAGG CGACTTGCTT 
GTTTCACGAT ATGTGCTTTG TCCGCAGTGT GTCAGGGATG CTGAGAGAAA TGGCTCACGG 
AGCCGAACTT CTTCTTCAGC GTCACACCGA AGATCACAGG ATGACGGAGA GCTTCCAATA 
ACCAGCAGCT CTCACATGAA AGGTTCTCGA ACAACAGGAG ACATTTCAAA ATGTCGCGGA 
GGAGTTGTTC ACTGTTTCGT TATTGAGGAA TGTATGCTGG CTGGAAGAGA GTACAACTGG 
GTAGAATGCC CATCTCATGG TGGTCTTCAT ATGAGAGAAC TTGCACCGGA TACTGTATTT 
GCTGATATCG AAAATGCTTT GACAATTCAC CCGGATCAGT TAAAACGAAG CAGAATGCTC 
GGAAGAGGAG CGTTTGGTTT CGTTTTTCGG GCTACAGTAA GACAACCAAA TGGAGAACTT 
TGTGAAGTTG CTCAGAAAAT GTTGGAGCCT GTAGACCCAG GGCCTGGAGG CAGGCCTTCG 
GCTCTCGCAG CTTATAAGGC AGCAGCAGAC AAATGGAAGA GGGATAGTAT GGAATTTGCG 
TGTAGAGCAT ACTGTACTTC AAGACAAGAG CTCAGTTTAT TGAGTAGGAT GAAACATCCA 
AATGTTATTG GACTAGTTGG AGTTTGCACT TTTCCATTGT CTCTTGTTGT AGAGCTTGCA 
CCTCTCGGAG CACTTAACCA GCTGCTGGGA TCTCATCGAA AAGCTGGAAC TAAGCTCTCA 
CTTGGAGTTA TCAAAGAGTC TGCAGTTCAA GTTGCTCGAG CACTGGAATA TCTTCATTCT 
GCACATATTA TCTACAGAGA TCTCAAGTCG GAAAATGTAC TTGGATGGAG ATTTCCTGCT 
CCATTCAGTC CACAGACTGA TGTTCTATTG AAACTTGGAG ATTACGGGAT ATCAAGAAGT 
GTACTTCCAT CAGGTGGAGC AAAGGGATTC GGAGGAACGG AAGGATTTAT GGCTCCAGAA 
ATTGTAAGGT TCAATGGAGA AGAGGAATAT ACACAAAAAG TGGATTGCTT TTCGTTTGGA 
ATGTTCCTCT ATGAACTTCT CACTCTGAAA TTCCCATTCG AAAGTGAAGA GCATGTGAAA 
GAAAGAATGC TCGACGGAGC CCGTCCCGTA CTTTTACCGC ACGAGCTTCT CCTTCCAACT 
CCAATGCTGG ATCTTCTCGT TCACTGCTGG TCGGCTCATC CAGAATCCCG ACCTTCGTCG 
TCTCAACTAG TCGGATTCTG TGCAGCTCCA GAATTCACAC ATCTTCTTGA TGTTTGTGAA 
CTTGGAGAAG CTCTACCACC AACTCAGCTG ATGGCAGTAG GAATAACGGA CGAAATTGAT 
GATCCGGATG ACTTTGAAGC TCAACTTTGG CTCAGTGGAC GGGAAATGGT AGTCATGGGA 
TGCACACAAT ACGGATTTGT TGACCAAAAA TCGATTGAGT TACCTCATCG AGGCAAGTAT 
GTGAGCAAGG TTCGAGATTC TGTGTGGTCT TGCGACGAAT GTGGACAGGT TACTGTATAT 
GGAATATCTC TACATGAAAC CGGGCACTTG CAGTTGCCAT CACTTAATGG AACTTTGATT 
TGTGCACCTG AACTGATAAG CAACGACGTG CTTATACTAA TTTCTGATAA ACAAATTGTG 
CTTTTGAAGC TTTCCGAATC AAATTCCGTA TCCCATCTTG GAACAATCGA TTCACCGTAT 
GAAATTCGGA CAGCCACATT CCTGGGCAAT GGTTCAACCC GTCAAATATG GGCGGGACAT 
TCAGAAGGAC GAATTTCAAT TCATCATATA GCTTCGAACG ATTCTTTCAG CTTCTCGTCA 
AGTCTTTATC TTCCAGATGA TAAGTGTATT GTCAGACAGT TAGTTGGCTC GAAAGATGCA 
CAAAAAGTGT GGATCGCATT AGAGAAAAGC TCGAAAGTTC AAATGGTTGA AGTGGAAAAA 
CGACAAGTGA CTGGATCACT GGATATTCGA AAAGTGATGC CTGGAAGTGA AACAATTCAT 
ACAATTGATA TGGAAATGGC CAGTCAGAAT TATGTTACAT GTATTGGTTT ATTGGAAAGA 
AATGACGGAG ATCAACTGTA CATTGGAACT TCCAAAGGGC TGCTAGTCAT AGCTCACGCT 
ACTACACTTC AACCATTGTC AGCATGCCGT CCATTTGAAG GAGACATCAC ATCAATTTGT 
ATATTGGAAG AACCATCCCG TGAAGAAGAA AATACACGTG GAAAGGCTAC CACATTGAGC 
ACAGCTTCAT CAGAAAGTGG TCTTGGATGG GTACGGGAAA GAGTCAGCGA AACTGTTGAT 
AGATTCCGCT CGAGCCCAGC TACAGTAGAA ACTCAGGGAG CCGCTCTTGT TGTGTGCATT 
GGACGACAAT TCAGAAGCCT TTCACATCGT TTCGTGGCTG AGGAGAAGCT TGCCGACGTC 
TATTCCATTG CTGTCTGGAG GACCGAGGAA TGGGCTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492839.4
CDS	1..7182
Translation	MDLSSGGPSSSSDVASELDNSDAMQLVRQAVLFENVELLADLFKVNPWVWNRVDRHGRTPLMLAAHNGKLDSLRTILMLSPNSLNLVNDRGKTALHMAAESGETSIVLELVELGSDPMKSDNEGHCALELAQMAGHNEVAAKLIDAIQKESEDLNEAHTMIISACISGSADVVYEISRRFMEKKQSREILFNGRNEEDETALLIACTNGHIEIVRHLLQFEEHLLQSHVSKDTVIHAAVSSQNVEVLQLCLEKFPQLVKSTNNEGSTCLHWAARCGSSECVSTILNFPFPSEFIIEIDTVGAPAYQLALDVNEVDGECRTAMYLAVAEGHLEVVKAMTDFKCTSIDGRQRCPFQLDVYCTRGRTPFMLAAFNQNLPLMTLLLDAGADVNLPLAVLDTEYSVEEGRCIGSGALVEAVRSDGLHIVHFLLDRGALDTDNKALRLAAQGKNEKLIRVFLVRLVFADPEYKINKKNIDVGQIQVGQSLLPSSLCPSKAAQLNWNSANLEQLQSDWFVAAALHVNPRLRTTRLSLAAITRVDLSDNRLNTFPSILFQMPSLRSLNLADNSIRKIEIPTYYISSTSLEILNLRNNQLECIAIQFLSSLPQLQQLDVSKNELSQLPEYIWLCPALKELNASYNRLSTLPMVARASRGERPRLNNSNNNFNTQSPTQESNPIVVDDPPNVTSNPLRRQNVWQASINLSKVDDDSLFPDFPVTSSNTLTTINLSFNKFHTFPFCLACTCPRLLILNMSNNSMTSLPPMACVPAHLRTLDLSYNKIQESFIEASPLHVVCHAVPPTTSNGSMLPKRRNSPARQHRSRSKSAVRSQRSLSVSRHHALIDPQKEEESCVHKRHDSLEWLKTLQLAGNRLRSISVTNAASKVLLPALNVMDISDNKLLQAPPDVARLTLLSMLNLSGNTAIKELPPDYGMLSRLWSLSLKGCSLKEPLESMVNVENCKTVEIVAYLKTILEESKTYHHLRLMILGSDGVGKSVIWDALCKEAVQKRQPIHSETGVIRQAEWKFEAKRSKGDKNLGPVGFSVIDFGGQREYHSTHQYFLSKRSLNLVLWKITDGDEALAQLDTWLVNIHARAPNSTVILVGTNLDQVASNSSKFGPGYIDIMEQKVRTRYMVADADKSGLPRIVDVILINSTSRNDVKALLNTIYRTAWEVRMGKERAMEQQIPSSYIALMKVTKELGVEFRKEGQPAVMTVEAYRERVKKRMISKFGRPFRDDIEFYAACTFLHDCGELVRFEDATLRDLIFVDPLWLAEFLTSVVILRSPNLPAGLLSTDAINPHTRSFKSGALLMLKTQLLDLLHKFELALATQPRQLLIPSLLPDEYRLRSDFLASAVKIRMKMSQWNVRCPSPAGSPTKSPLRRTSPTDQNGVGSEDVMLQFTYDDDQLLRRIYALAYIPSGFWSRLVTRIVGDKNVCAAIESIFMTTSADRAKIADIATKHAKAEWVVWQTGIELHVKGHSLFTLKQFLPLAEVRDIDYSAIDMRAKDEQKRWRTWNQPSHRPIVEMVVNSLSISAASQHGRKLSMKTDVEGRSRLLAMISDLLDTLLEDWYPALGTRFVHSSEGDLLVSRYVLCPQCVRDAERNGSRSRTSSSASHRRSQDDGELPITSSSHMKGSRTTGDISKCRGGVVHCFVIEECMLAGREYNWVECPSHGGLHMRELAPDTVFADIENALTIHPDQLKRSRMLGRGAFGFVFRATVRQPNGELCEVAQKMLEPVDPGPGGRPSALAAYKAAADKWKRDSMEFACRAYCTSRQELSLLSRMKHPNVIGLVGVCTFPLSLVVELAPLGALNQLLGSHRKAGTKLSLGVIKESAVQVARALEYLHSAHIIYRDLKSENVLGWRFPAPFSPQTDVLLKLGDYGISRSVLPSGGAKGFGGTEGFMAPEIVRFNGEEEYTQKVDCFSFGMFLYELLTLKFPFESEEHVKERMLDGARPVLLPHELLLPTPMLDLLVHCWSAHPESRPSSSQLVGFCAAPEFTHLLDVCELGEALPPTQLMAVGITDEIDDPDDFEAQLWLSGREMVVMGCTQYGFVDQKSIELPHRGKYVSKVRDSVWSCDECGQVTVYGISLHETGHLQLPSLNGTLICAPELISNDVLILISDKQIVLLKLSESNSVSHLGTIDSPYEIRTATFLGNGSTRQIWAGHSEGRISIHHIASNDSFSFSSSLYLPDDKCIVRQLVGSKDAQKVWIALEKSSKVQMVEVEKRQVTGSLDIRKVMPGSETIHTIDMEMASQNYVTCIGLLERNDGDQLYIGTSKGLLVIAHATTLQPLSACRPFEGDITSICILEEPSREEENTRGKATTLSTASSESGLGWVRERVSETVDRFRSSPATVETQGAALVVCIGRQFRSLSHRFVAEEKLADVYSIAVWRTEEWAL

Target ORF information:

RefSeq Version	NM_060438.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Leucine-rich repeat serine/threonine-protein kinase 1 (lrk-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060438.6

ORF Insert Sequence:

ATGGACCTCT CAAGTGGGGG TCCGTCATCT TCAAGCGATG TAGCTTCAGA ATTGGATAAT 
TCGGATGCAA TGCAACTGGT TAGACAGGCA GTATTATTCG AGAACGTGGA GCTGCTGGCC 
GACTTGTTCA AAGTGAATCC TTGGGTATGG AATCGGGTTG ATCGACATGG AAGGACACCG 
TTAATGTTAG CTGCTCATAA TGGGAAGCTG GATAGTTTGA GGACTATTTT AATGCTTAGT 
CCTAATTCTC TAAATCTTGT GAACGATCGT GGTAAGACCG CACTTCATAT GGCAGCCGAA 
TCAGGTGAAA CTTCGATTGT TTTGGAGCTT GTCGAACTGG GCTCCGATCC AATGAAATCT 
GATAATGAAG GACATTGTGC ATTGGAGTTA GCTCAAATGG CTGGACATAA TGAGGTTGCC 
GCAAAGCTTA TTGATGCTAT TCAAAAAGAA AGTGAAGATC TAAATGAAGC CCACACAATG 
ATCATCAGTG CATGCATATC TGGAAGTGCT GACGTTGTTT ATGAAATTTC AAGGAGATTT 
ATGGAAAAAA AACAAAGCCG CGAAATATTA TTCAACGGTC GAAATGAAGA GGATGAGACT 
GCACTCTTGA TTGCCTGCAC AAACGGTCAT ATCGAAATCG TCCGTCACTT ATTACAATTC 
GAAGAGCATC TTCTACAAAG CCACGTATCC AAGGATACAG TAATCCATGC GGCAGTGTCG 
TCACAGAATG TGGAAGTGTT GCAGCTGTGT TTGGAGAAAT TCCCTCAACT CGTCAAATCT 
ACCAACAACG AGGGCTCTAC GTGTCTACAT TGGGCCGCCC GTTGTGGATC TTCGGAATGT 
GTTTCCACAA TTCTAAACTT TCCATTCCCA TCTGAATTTA TAATCGAAAT CGACACAGTT 
GGAGCACCAG CTTATCAGCT TGCTCTAGAC GTGAATGAAG TTGACGGAGA GTGTCGAACG 
GCAATGTATC TAGCGGTTGC CGAAGGACAT TTGGAAGTTG TCAAGGCGAT GACTGATTTC 
AAATGCACAT CAATCGACGG AAGACAACGT TGCCCGTTTC AACTTGACGT CTACTGTACT 
CGAGGACGAA CACCATTCAT GTTGGCCGCT TTTAATCAGA ATCTTCCATT GATGACGCTT 
CTTCTCGACG CTGGTGCAGA TGTCAACTTG CCGCTGGCAG TCTTGGACAC AGAGTACAGC 
GTAGAAGAAG GTCGATGCAT TGGCTCGGGT GCTCTCGTTG AAGCTGTACG ATCTGATGGA 
CTACACATTG TGCACTTTCT GTTAGACCGT GGAGCTCTGG ACACTGATAA CAAGGCTCTT 
CGACTTGCTG CTCAGGGGAA GAACGAGAAG CTCATTCGGG TTTTTCTGGT TCGCCTGGTT 
TTTGCGGACC CTGAATATAA GATCAATAAG AAAAACATTG ATGTAGGTCA GATTCAAGTT 
GGTCAGAGTC TGCTACCAAG TTCACTGTGC CCATCAAAAG CCGCCCAACT GAATTGGAAT 
TCAGCCAATT TGGAGCAACT TCAATCGGAT TGGTTTGTGG CGGCAGCTCT ACACGTGAAC 
CCGAGACTCC GAACAACGAG GTTATCACTG GCAGCAATCA CTCGGGTCGA TTTATCGGAC 
AATCGTCTCA ACACATTCCC AAGTATTCTA TTCCAAATGC CATCGCTCAG ATCTCTAAAT 
CTAGCTGATA ATAGTATACG TAAAATTGAA ATTCCAACTT ATTACATTAG TTCAACATCG 
CTAGAGATTC TCAATTTACG GAATAATCAA CTCGAGTGTA TAGCCATTCA ATTTCTATCA 
TCTCTTCCAC AATTGCAACA ATTGGATGTA TCGAAGAACG AGTTGAGCCA ACTTCCCGAG 
TATATATGGT TGTGTCCAGC GTTGAAAGAG CTCAATGCAT CCTATAATAG ACTGAGCACA 
CTTCCGATGG TTGCAAGAGC ATCAAGAGGA GAGAGACCAC GACTCAATAA TTCTAACAAC 
AATTTCAACA CCCAATCTCC AACACAAGAA TCGAATCCAA TCGTCGTTGA TGATCCTCCG 
AATGTCACTT CAAACCCTCT GCGTCGCCAA AATGTGTGGC AAGCGAGTAT CAATTTGTCA 
AAAGTCGACG ACGATTCCCT GTTCCCCGAC TTCCCGGTCA CTTCCAGTAA CACATTGACG 
ACGATAAATC TATCTTTCAA TAAATTCCAC ACATTCCCAT TCTGCCTGGC ATGCACATGT 
CCCCGGCTTC TGATTCTAAA CATGTCAAAC AACTCAATGA CGTCACTTCC ACCTATGGCT 
TGTGTACCTG CGCATCTGAG GACGTTGGAT CTGTCTTACA ATAAGATACA AGAAAGTTTC 
ATCGAAGCTT CACCTCTCCA CGTGGTCTGT CACGCGGTCC CACCGACAAC GTCTAATGGC 
TCAATGCTCC CAAAGCGAAG AAATTCTCCA GCTCGGCAAC ATAGAAGTCG TTCGAAGTCT 
GCAGTTAGAT CACAGAGAAG TCTCAGTGTT TCTCGCCATC ATGCGCTAAT TGATCCACAA 
AAAGAAGAAG AAAGTTGTGT TCATAAGCGT CACGATTCAT TAGAATGGCT CAAGACACTA 
CAATTGGCTG GAAATCGACT CCGATCAATT TCAGTGACAA ATGCAGCTTC AAAAGTTCTC 
CTTCCAGCTC TGAACGTGAT GGATATCAGT GACAACAAAT TGCTACAAGC ACCACCGGAT 
GTTGCACGGC TCACACTTCT TTCAATGCTC AACTTATCAG GAAATACTGC AATTAAAGAA 
CTACCACCAG ATTATGGAAT GCTCTCTCGT CTTTGGAGTC TATCTTTGAA AGGATGCTCA 
CTGAAAGAAC CACTTGAATC AATGGTTAAT GTGGAGAACT GCAAGACAGT GGAAATTGTG 
GCGTATTTGA AGACAATTCT AGAAGAATCC AAGACATACC ATCATTTGAG ATTAATGATT 
TTGGGAAGTG ATGGCGTTGG AAAATCTGTT ATTTGGGATG CACTGTGCAA GGAAGCAGTA 
CAGAAACGGC AGCCTATTCA CTCGGAAACT GGTGTTATTC GGCAAGCTGA ATGGAAGTTC 
GAAGCGAAAC GATCTAAAGG CGATAAGAAT CTTGGGCCTG TCGGATTTTC TGTAATCGAC 
TTTGGAGGTC AACGAGAATA TCATTCCACT CATCAATATT TCCTGAGCAA ACGATCCTTG 
AACTTGGTTC TTTGGAAGAT CACAGACGGG GATGAAGCAC TGGCGCAGCT TGATACTTGG 
CTAGTTAATA TCCATGCAAG AGCGCCAAAC TCGACAGTAA TACTAGTGGG AACCAATTTG 
GATCAAGTGG CATCGAATAG TTCGAAATTT GGACCTGGAT ACATAGACAT CATGGAGCAA 
AAAGTGCGGA CCCGTTATAT GGTTGCAGAT GCGGATAAGT CAGGATTGCC ACGTATTGTT 
GATGTAATTT TGATCAATTC AACGTCAAGA AATGATGTAA AAGCACTGCT AAACACAATA 
TATAGAACTG CGTGGGAAGT ACGAATGGGA AAAGAAAGAG CTATGGAACA ACAGATTCCA 
TCTTCTTACA TTGCTTTGAT GAAAGTAACA AAAGAATTAG GCGTCGAGTT TCGGAAAGAG 
GGGCAGCCGG CTGTAATGAC TGTAGAGGCA TATAGAGAAA GAGTCAAGAA AAGGATGATT 
AGCAAGTTTG GCAGACCGTT TAGGGACGAC ATTGAATTCT ACGCCGCATG CACATTCCTT 
CACGATTGTG GTGAATTAGT TCGATTTGAG GATGCCACTC TTCGGGATCT GATTTTTGTA 
GATCCGCTGT GGCTCGCCGA GTTTCTCACT TCTGTCGTCA TTCTAAGAAG CCCAAATCTT 
CCAGCTGGAC TTCTTTCAAC AGACGCTATA AATCCGCATA CTCGTTCATT CAAATCAGGA 
GCACTTTTAA TGCTCAAAAC ACAGCTTTTG GATCTTCTTC ACAAATTCGA ATTGGCACTT 
GCTACCCAGC CTCGACAGCT TCTGATTCCA AGCCTGCTAC CAGATGAGTA CAGGCTACGA 
TCTGATTTCT TAGCATCTGC AGTTAAAATC CGAATGAAAA TGTCACAGTG GAATGTCAGA 
TGTCCAAGTC CCGCTGGATC ACCGACCAAA TCCCCACTCC GTCGAACATC ACCTACGGAT 
CAGAACGGTG TTGGATCAGA GGATGTTATG CTTCAATTCA CTTATGACGA TGATCAATTG 
TTAAGAAGGA TTTATGCGTT GGCTTATATA CCTTCAGGAT TTTGGAGTCG CCTAGTGACT 
CGTATAGTTG GAGATAAAAA CGTGTGTGCA GCAATTGAAA GTATTTTCAT GACAACAAGT 
GCGGATCGAG CAAAGATTGC AGATATAGCC ACTAAGCATG CAAAAGCTGA ATGGGTTGTG 
TGGCAAACTG GAATAGAGCT TCATGTTAAA GGACATTCAC TTTTCACTCT GAAACAGTTT 
CTACCGTTGG CAGAGGTTAG AGACATTGAC TACAGCGCCA TAGACATGCG CGCAAAGGAT 
GAACAAAAAC GATGGAGGAC ATGGAATCAA CCTTCACACC GCCCAATTGT CGAAATGGTT 
GTTAACTCAT TGAGCATTTC AGCAGCTTCA CAACACGGAA GAAAACTTTC AATGAAAACA 
GATGTGGAAG GAAGAAGCCG ATTATTGGCA ATGATATCAG ATTTATTGGA CACGTTACTC 
GAAGACTGGT ATCCGGCTCT TGGAACTCGA TTTGTTCACA GTTCGGAAGG CGACTTGCTT 
GTTTCACGAT ATGTGCTTTG TCCGCAGTGT GTCAGGGATG CTGAGAGAAA TGGCTCACGG 
AGCCGAACTT CTTCTTCAGC GTCACACCGA AGATCACAGG ATGACGGAGA GCTTCCAATA 
ACCAGCAGCT CTCACATGAA AGGTTCTCGA ACAACAGGAG ACATTTCAAA ATGTCGCGGA 
GGAGTTGTTC ACTGTTTCGT TATTGAGGAA TGTATGCTGG CTGGAAGAGA GTACAACTGG 
GTAGAATGCC CATCTCATGG TGGTCTTCAT ATGAGAGAAC TTGCACCGGA TACTGTATTT 
GCTGATATCG AAAATGCTTT GACAATTCAC CCGGATCAGT TAAAACGAAG CAGAATGCTC 
GGAAGAGGAG CGTTTGGTTT CGTTTTTCGG GCTACAGTAA GACAACCAAA TGGAGAACTT 
TGTGAAGTTG CTCAGAAAAT GTTGGAGCCT GTAGACCCAG GGCCTGGAGG CAGGCCTTCG 
GCTCTCGCAG CTTATAAGGC AGCAGCAGAC AAATGGAAGA GGGATAGTAT GGAATTTGCG 
TGTAGAGCAT ACTGTACTTC AAGACAAGAG CTCAGTTTAT TGAGTAGGAT GAAACATCCA 
AATGTTATTG GACTAGTTGG AGTTTGCACT TTTCCATTGT CTCTTGTTGT AGAGCTTGCA 
CCTCTCGGAG CACTTAACCA GCTGCTGGGA TCTCATCGAA AAGCTGGAAC TAAGCTCTCA 
CTTGGAGTTA TCAAAGAGTC TGCAGTTCAA GTTGCTCGAG CACTGGAATA TCTTCATTCT 
GCACATATTA TCTACAGAGA TCTCAAGTCG GAAAATGTAC TTGGATGGAG ATTTCCTGCT 
CCATTCAGTC CACAGACTGA TGTTCTATTG AAACTTGGAG ATTACGGGAT ATCAAGAAGT 
GTACTTCCAT CAGGTGGAGC AAAGGGATTC GGAGGAACGG AAGGATTTAT GGCTCCAGAA 
ATTGTAAGGT TCAATGGAGA AGAGGAATAT ACACAAAAAG TGGATTGCTT TTCGTTTGGA 
ATGTTCCTCT ATGAACTTCT CACTCTGAAA TTCCCATTCG AAAGTGAAGA GCATGTGAAA 
GAAAGAATGC TCGACGGAGC CCGTCCCGTA CTTTTACCGC ACGAGCTTCT CCTTCCAACT 
CCAATGCTGG ATCTTCTCGT TCACTGCTGG TCGGCTCATC CAGAATCCCG ACCTTCGTCG 
TCTCAACTAG TCGGATTCTG TGCAGCTCCA GAATTCACAC ATCTTCTTGA TGTTTGTGAA 
CTTGGAGAAG CTCTACCACC AACTCAGCTG ATGGCAGTAG GAATAACGGA CGAAATTGAT 
GATCCGGATG ACTTTGAAGC TCAACTTTGG CTCAGTGGAC GGGAAATGGT AGTCATGGGA 
TGCACACAAT ACGGATTTGT TGACCAAAAA TCGATTGAGT TACCTCATCG AGGCAAGTAT 
GTGAGCAAGG TTCGAGATTC TGTGTGGTCT TGCGACGAAT GTGGACAGGT TACTGTATAT 
GGAATATCTC TACATGAAAC CGGGCACTTG CAGTTGCCAT CACTTAATGG AACTTTGATT 
TGTGCACCTG AACTGATAAG CAACGACGTG CTTATACTAA TTTCTGATAA ACAAATTGTG 
CTTTTGAAGC TTTCCGAATC AAATTCCGTA TCCCATCTTG GAACAATCGA TTCACCGTAT 
GAAATTCGGA CAGCCACATT CCTGGGCAAT GGTTCAACCC GTCAAATATG GGCGGGACAT 
TCAGAAGGAC GAATTTCAAT TCATCATATA GCTTCGAACG ATTCTTTCAG CTTCTCGTCA 
AGTCTTTATC TTCCAGATGA TAAGTGTATT GTCAGACAGT TAGTTGGCTC GAAAGATGCA 
CAAAAAGTGT GGATCGCATT AGAGAAAAGC TCGAAAGTTC AAATGGTTGA AGTGGAAAAA 
CGACAAGTGA CTGGATCACT GGATATTCGA AAAGTGATGC CTGGAAGTGA AACAATTCAT 
ACAATTGATA TGGAAATGGC CAGTCAGAAT TATGTTACAT GTATTGGTTT ATTGGAAAGA 
AATGACGGAG ATCAACTGTA CATTGGAACT TCCAAAGGGC TGCTAGTCAT AGCTCACGCT 
ACTACACTTC AACCATTGTC AGCATGCCGT CCATTTGAAG GAGACATCAC ATCAATTTGT 
ATATTGGAAG AACCATCCCG TGAAGAAGAA AATACACGTG GAAAGGCTAC CACATTGAGC 
ACAGCTTCAT CAGAAAGTGG TCTTGGATGG GTACGGGAAA GAGTCAGCGA AACTGTTGAT 
AGATTCCGCT CGAGCCCAGC TACAGTAGAA ACTCAGGGAG CCGCTCTTGT TGTGTGCATT 
GGACGACAAT TCAGAAGCCT TTCACATCGT TTCGTGGCTG AGGAGAAGCT TGCCGACGTC 
TATTCCATTG CTGTCTGGAG GACCGAGGAA TGGGCTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lrk-1 ( NM_060438.7 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492839.4 Caenorhabditis elegans Leucine-rich repeat serine/threonine-protein kinase 1 (lrk-1), mRNA.

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CloneID	OCa337000
Clone ID Related Accession (Same CDS sequence)	NM_060438.6 , NM_060438.7
Accession Version	NM_060438.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Leucine-rich repeat serine/threonine-protein kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_060438.6.

ATGGACCTCT CAAGTGGGGG TCCGTCATCT TCAAGCGATG TAGCTTCAGA ATTGGATAAT 
TCGGATGCAA TGCAACTGGT TAGACAGGCA GTATTATTCG AGAACGTGGA GCTGCTGGCC 
GACTTGTTCA AAGTGAATCC TTGGGTATGG AATCGGGTTG ATCGACATGG AAGGACACCG 
TTAATGTTAG CTGCTCATAA TGGGAAGCTG GATAGTTTGA GGACTATTTT AATGCTTAGT 
CCTAATTCTC TAAATCTTGT GAACGATCGT GGTAAGACCG CACTTCATAT GGCAGCCGAA 
TCAGGTGAAA CTTCGATTGT TTTGGAGCTT GTCGAACTGG GCTCCGATCC AATGAAATCT 
GATAATGAAG GACATTGTGC ATTGGAGTTA GCTCAAATGG CTGGACATAA TGAGGTTGCC 
GCAAAGCTTA TTGATGCTAT TCAAAAAGAA AGTGAAGATC TAAATGAAGC CCACACAATG 
ATCATCAGTG CATGCATATC TGGAAGTGCT GACGTTGTTT ATGAAATTTC AAGGAGATTT 
ATGGAAAAAA AACAAAGCCG CGAAATATTA TTCAACGGTC GAAATGAAGA GGATGAGACT 
GCACTCTTGA TTGCCTGCAC AAACGGTCAT ATCGAAATCG TCCGTCACTT ATTACAATTC 
GAAGAGCATC TTCTACAAAG CCACGTATCC AAGGATACAG TAATCCATGC GGCAGTGTCG 
TCACAGAATG TGGAAGTGTT GCAGCTGTGT TTGGAGAAAT TCCCTCAACT CGTCAAATCT 
ACCAACAACG AGGGCTCTAC GTGTCTACAT TGGGCCGCCC GTTGTGGATC TTCGGAATGT 
GTTTCCACAA TTCTAAACTT TCCATTCCCA TCTGAATTTA TAATCGAAAT CGACACAGTT 
GGAGCACCAG CTTATCAGCT TGCTCTAGAC GTGAATGAAG TTGACGGAGA GTGTCGAACG 
GCAATGTATC TAGCGGTTGC CGAAGGACAT TTGGAAGTTG TCAAGGCGAT GACTGATTTC 
AAATGCACAT CAATCGACGG AAGACAACGT TGCCCGTTTC AACTTGACGT CTACTGTACT 
CGAGGACGAA CACCATTCAT GTTGGCCGCT TTTAATCAGA ATCTTCCATT GATGACGCTT 
CTTCTCGACG CTGGTGCAGA TGTCAACTTG CCGCTGGCAG TCTTGGACAC AGAGTACAGC 
GTAGAAGAAG GTCGATGCAT TGGCTCGGGT GCTCTCGTTG AAGCTGTACG ATCTGATGGA 
CTACACATTG TGCACTTTCT GTTAGACCGT GGAGCTCTGG ACACTGATAA CAAGGCTCTT 
CGACTTGCTG CTCAGGGGAA GAACGAGAAG CTCATTCGGG TTTTTCTGGT TCGCCTGGTT 
TTTGCGGACC CTGAATATAA GATCAATAAG AAAAACATTG ATGTAGGTCA GATTCAAGTT 
GGTCAGAGTC TGCTACCAAG TTCACTGTGC CCATCAAAAG CCGCCCAACT GAATTGGAAT 
TCAGCCAATT TGGAGCAACT TCAATCGGAT TGGTTTGTGG CGGCAGCTCT ACACGTGAAC 
CCGAGACTCC GAACAACGAG GTTATCACTG GCAGCAATCA CTCGGGTCGA TTTATCGGAC 
AATCGTCTCA ACACATTCCC AAGTATTCTA TTCCAAATGC CATCGCTCAG ATCTCTAAAT 
CTAGCTGATA ATAGTATACG TAAAATTGAA ATTCCAACTT ATTACATTAG TTCAACATCG 
CTAGAGATTC TCAATTTACG GAATAATCAA CTCGAGTGTA TAGCCATTCA ATTTCTATCA 
TCTCTTCCAC AATTGCAACA ATTGGATGTA TCGAAGAACG AGTTGAGCCA ACTTCCCGAG 
TATATATGGT TGTGTCCAGC GTTGAAAGAG CTCAATGCAT CCTATAATAG ACTGAGCACA 
CTTCCGATGG TTGCAAGAGC ATCAAGAGGA GAGAGACCAC GACTCAATAA TTCTAACAAC 
AATTTCAACA CCCAATCTCC AACACAAGAA TCGAATCCAA TCGTCGTTGA TGATCCTCCG 
AATGTCACTT CAAACCCTCT GCGTCGCCAA AATGTGTGGC AAGCGAGTAT CAATTTGTCA 
AAAGTCGACG ACGATTCCCT GTTCCCCGAC TTCCCGGTCA CTTCCAGTAA CACATTGACG 
ACGATAAATC TATCTTTCAA TAAATTCCAC ACATTCCCAT TCTGCCTGGC ATGCACATGT 
CCCCGGCTTC TGATTCTAAA CATGTCAAAC AACTCAATGA CGTCACTTCC ACCTATGGCT 
TGTGTACCTG CGCATCTGAG GACGTTGGAT CTGTCTTACA ATAAGATACA AGAAAGTTTC 
ATCGAAGCTT CACCTCTCCA CGTGGTCTGT CACGCGGTCC CACCGACAAC GTCTAATGGC 
TCAATGCTCC CAAAGCGAAG AAATTCTCCA GCTCGGCAAC ATAGAAGTCG TTCGAAGTCT 
GCAGTTAGAT CACAGAGAAG TCTCAGTGTT TCTCGCCATC ATGCGCTAAT TGATCCACAA 
AAAGAAGAAG AAAGTTGTGT TCATAAGCGT CACGATTCAT TAGAATGGCT CAAGACACTA 
CAATTGGCTG GAAATCGACT CCGATCAATT TCAGTGACAA ATGCAGCTTC AAAAGTTCTC 
CTTCCAGCTC TGAACGTGAT GGATATCAGT GACAACAAAT TGCTACAAGC ACCACCGGAT 
GTTGCACGGC TCACACTTCT TTCAATGCTC AACTTATCAG GAAATACTGC AATTAAAGAA 
CTACCACCAG ATTATGGAAT GCTCTCTCGT CTTTGGAGTC TATCTTTGAA AGGATGCTCA 
CTGAAAGAAC CACTTGAATC AATGGTTAAT GTGGAGAACT GCAAGACAGT GGAAATTGTG 
GCGTATTTGA AGACAATTCT AGAAGAATCC AAGACATACC ATCATTTGAG ATTAATGATT 
TTGGGAAGTG ATGGCGTTGG AAAATCTGTT ATTTGGGATG CACTGTGCAA GGAAGCAGTA 
CAGAAACGGC AGCCTATTCA CTCGGAAACT GGTGTTATTC GGCAAGCTGA ATGGAAGTTC 
GAAGCGAAAC GATCTAAAGG CGATAAGAAT CTTGGGCCTG TCGGATTTTC TGTAATCGAC 
TTTGGAGGTC AACGAGAATA TCATTCCACT CATCAATATT TCCTGAGCAA ACGATCCTTG 
AACTTGGTTC TTTGGAAGAT CACAGACGGG GATGAAGCAC TGGCGCAGCT TGATACTTGG 
CTAGTTAATA TCCATGCAAG AGCGCCAAAC TCGACAGTAA TACTAGTGGG AACCAATTTG 
GATCAAGTGG CATCGAATAG TTCGAAATTT GGACCTGGAT ACATAGACAT CATGGAGCAA 
AAAGTGCGGA CCCGTTATAT GGTTGCAGAT GCGGATAAGT CAGGATTGCC ACGTATTGTT 
GATGTAATTT TGATCAATTC AACGTCAAGA AATGATGTAA AAGCACTGCT AAACACAATA 
TATAGAACTG CGTGGGAAGT ACGAATGGGA AAAGAAAGAG CTATGGAACA ACAGATTCCA 
TCTTCTTACA TTGCTTTGAT GAAAGTAACA AAAGAATTAG GCGTCGAGTT TCGGAAAGAG 
GGGCAGCCGG CTGTAATGAC TGTAGAGGCA TATAGAGAAA GAGTCAAGAA AAGGATGATT 
AGCAAGTTTG GCAGACCGTT TAGGGACGAC ATTGAATTCT ACGCCGCATG CACATTCCTT 
CACGATTGTG GTGAATTAGT TCGATTTGAG GATGCCACTC TTCGGGATCT GATTTTTGTA 
GATCCGCTGT GGCTCGCCGA GTTTCTCACT TCTGTCGTCA TTCTAAGAAG CCCAAATCTT 
CCAGCTGGAC TTCTTTCAAC AGACGCTATA AATCCGCATA CTCGTTCATT CAAATCAGGA 
GCACTTTTAA TGCTCAAAAC ACAGCTTTTG GATCTTCTTC ACAAATTCGA ATTGGCACTT 
GCTACCCAGC CTCGACAGCT TCTGATTCCA AGCCTGCTAC CAGATGAGTA CAGGCTACGA 
TCTGATTTCT TAGCATCTGC AGTTAAAATC CGAATGAAAA TGTCACAGTG GAATGTCAGA 
TGTCCAAGTC CCGCTGGATC ACCGACCAAA TCCCCACTCC GTCGAACATC ACCTACGGAT 
CAGAACGGTG TTGGATCAGA GGATGTTATG CTTCAATTCA CTTATGACGA TGATCAATTG 
TTAAGAAGGA TTTATGCGTT GGCTTATATA CCTTCAGGAT TTTGGAGTCG CCTAGTGACT 
CGTATAGTTG GAGATAAAAA CGTGTGTGCA GCAATTGAAA GTATTTTCAT GACAACAAGT 
GCGGATCGAG CAAAGATTGC AGATATAGCC ACTAAGCATG CAAAAGCTGA ATGGGTTGTG 
TGGCAAACTG GAATAGAGCT TCATGTTAAA GGACATTCAC TTTTCACTCT GAAACAGTTT 
CTACCGTTGG CAGAGGTTAG AGACATTGAC TACAGCGCCA TAGACATGCG CGCAAAGGAT 
GAACAAAAAC GATGGAGGAC ATGGAATCAA CCTTCACACC GCCCAATTGT CGAAATGGTT 
GTTAACTCAT TGAGCATTTC AGCAGCTTCA CAACACGGAA GAAAACTTTC AATGAAAACA 
GATGTGGAAG GAAGAAGCCG ATTATTGGCA ATGATATCAG ATTTATTGGA CACGTTACTC 
GAAGACTGGT ATCCGGCTCT TGGAACTCGA TTTGTTCACA GTTCGGAAGG CGACTTGCTT 
GTTTCACGAT ATGTGCTTTG TCCGCAGTGT GTCAGGGATG CTGAGAGAAA TGGCTCACGG 
AGCCGAACTT CTTCTTCAGC GTCACACCGA AGATCACAGG ATGACGGAGA GCTTCCAATA 
ACCAGCAGCT CTCACATGAA AGGTTCTCGA ACAACAGGAG ACATTTCAAA ATGTCGCGGA 
GGAGTTGTTC ACTGTTTCGT TATTGAGGAA TGTATGCTGG CTGGAAGAGA GTACAACTGG 
GTAGAATGCC CATCTCATGG TGGTCTTCAT ATGAGAGAAC TTGCACCGGA TACTGTATTT 
GCTGATATCG AAAATGCTTT GACAATTCAC CCGGATCAGT TAAAACGAAG CAGAATGCTC 
GGAAGAGGAG CGTTTGGTTT CGTTTTTCGG GCTACAGTAA GACAACCAAA TGGAGAACTT 
TGTGAAGTTG CTCAGAAAAT GTTGGAGCCT GTAGACCCAG GGCCTGGAGG CAGGCCTTCG 
GCTCTCGCAG CTTATAAGGC AGCAGCAGAC AAATGGAAGA GGGATAGTAT GGAATTTGCG 
TGTAGAGCAT ACTGTACTTC AAGACAAGAG CTCAGTTTAT TGAGTAGGAT GAAACATCCA 
AATGTTATTG GACTAGTTGG AGTTTGCACT TTTCCATTGT CTCTTGTTGT AGAGCTTGCA 
CCTCTCGGAG CACTTAACCA GCTGCTGGGA TCTCATCGAA AAGCTGGAAC TAAGCTCTCA 
CTTGGAGTTA TCAAAGAGTC TGCAGTTCAA GTTGCTCGAG CACTGGAATA TCTTCATTCT 
GCACATATTA TCTACAGAGA TCTCAAGTCG GAAAATGTAC TTGGATGGAG ATTTCCTGCT 
CCATTCAGTC CACAGACTGA TGTTCTATTG AAACTTGGAG ATTACGGGAT ATCAAGAAGT 
GTACTTCCAT CAGGTGGAGC AAAGGGATTC GGAGGAACGG AAGGATTTAT GGCTCCAGAA 
ATTGTAAGGT TCAATGGAGA AGAGGAATAT ACACAAAAAG TGGATTGCTT TTCGTTTGGA 
ATGTTCCTCT ATGAACTTCT CACTCTGAAA TTCCCATTCG AAAGTGAAGA GCATGTGAAA 
GAAAGAATGC TCGACGGAGC CCGTCCCGTA CTTTTACCGC ACGAGCTTCT CCTTCCAACT 
CCAATGCTGG ATCTTCTCGT TCACTGCTGG TCGGCTCATC CAGAATCCCG ACCTTCGTCG 
TCTCAACTAG TCGGATTCTG TGCAGCTCCA GAATTCACAC ATCTTCTTGA TGTTTGTGAA 
CTTGGAGAAG CTCTACCACC AACTCAGCTG ATGGCAGTAG GAATAACGGA CGAAATTGAT 
GATCCGGATG ACTTTGAAGC TCAACTTTGG CTCAGTGGAC GGGAAATGGT AGTCATGGGA 
TGCACACAAT ACGGATTTGT TGACCAAAAA TCGATTGAGT TACCTCATCG AGGCAAGTAT 
GTGAGCAAGG TTCGAGATTC TGTGTGGTCT TGCGACGAAT GTGGACAGGT TACTGTATAT 
GGAATATCTC TACATGAAAC CGGGCACTTG CAGTTGCCAT CACTTAATGG AACTTTGATT 
TGTGCACCTG AACTGATAAG CAACGACGTG CTTATACTAA TTTCTGATAA ACAAATTGTG 
CTTTTGAAGC TTTCCGAATC AAATTCCGTA TCCCATCTTG GAACAATCGA TTCACCGTAT 
GAAATTCGGA CAGCCACATT CCTGGGCAAT GGTTCAACCC GTCAAATATG GGCGGGACAT 
TCAGAAGGAC GAATTTCAAT TCATCATATA GCTTCGAACG ATTCTTTCAG CTTCTCGTCA 
AGTCTTTATC TTCCAGATGA TAAGTGTATT GTCAGACAGT TAGTTGGCTC GAAAGATGCA 
CAAAAAGTGT GGATCGCATT AGAGAAAAGC TCGAAAGTTC AAATGGTTGA AGTGGAAAAA 
CGACAAGTGA CTGGATCACT GGATATTCGA AAAGTGATGC CTGGAAGTGA AACAATTCAT 
ACAATTGATA TGGAAATGGC CAGTCAGAAT TATGTTACAT GTATTGGTTT ATTGGAAAGA 
AATGACGGAG ATCAACTGTA CATTGGAACT TCCAAAGGGC TGCTAGTCAT AGCTCACGCT 
ACTACACTTC AACCATTGTC AGCATGCCGT CCATTTGAAG GAGACATCAC ATCAATTTGT 
ATATTGGAAG AACCATCCCG TGAAGAAGAA AATACACGTG GAAAGGCTAC CACATTGAGC 
ACAGCTTCAT CAGAAAGTGG TCTTGGATGG GTACGGGAAA GAGTCAGCGA AACTGTTGAT 
AGATTCCGCT CGAGCCCAGC TACAGTAGAA ACTCAGGGAG CCGCTCTTGT TGTGTGCATT 
GGACGACAAT TCAGAAGCCT TTCACATCGT TTCGTGGCTG AGGAGAAGCT TGCCGACGTC 
TATTCCATTG CTGTCTGGAG GACCGAGGAA TGGGCTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492839.4
CDS	15..7196
Translation	MDLSSGGPSSSSDVASELDNSDAMQLVRQAVLFENVELLADLFKVNPWVWNRVDRHGRTPLMLAAHNGKLDSLRTILMLSPNSLNLVNDRGKTALHMAAESGETSIVLELVELGSDPMKSDNEGHCALELAQMAGHNEVAAKLIDAIQKESEDLNEAHTMIISACISGSADVVYEISRRFMEKKQSREILFNGRNEEDETALLIACTNGHIEIVRHLLQFEEHLLQSHVSKDTVIHAAVSSQNVEVLQLCLEKFPQLVKSTNNEGSTCLHWAARCGSSECVSTILNFPFPSEFIIEIDTVGAPAYQLALDVNEVDGECRTAMYLAVAEGHLEVVKAMTDFKCTSIDGRQRCPFQLDVYCTRGRTPFMLAAFNQNLPLMTLLLDAGADVNLPLAVLDTEYSVEEGRCIGSGALVEAVRSDGLHIVHFLLDRGALDTDNKALRLAAQGKNEKLIRVFLVRLVFADPEYKINKKNIDVGQIQVGQSLLPSSLCPSKAAQLNWNSANLEQLQSDWFVAAALHVNPRLRTTRLSLAAITRVDLSDNRLNTFPSILFQMPSLRSLNLADNSIRKIEIPTYYISSTSLEILNLRNNQLECIAIQFLSSLPQLQQLDVSKNELSQLPEYIWLCPALKELNASYNRLSTLPMVARASRGERPRLNNSNNNFNTQSPTQESNPIVVDDPPNVTSNPLRRQNVWQASINLSKVDDDSLFPDFPVTSSNTLTTINLSFNKFHTFPFCLACTCPRLLILNMSNNSMTSLPPMACVPAHLRTLDLSYNKIQESFIEASPLHVVCHAVPPTTSNGSMLPKRRNSPARQHRSRSKSAVRSQRSLSVSRHHALIDPQKEEESCVHKRHDSLEWLKTLQLAGNRLRSISVTNAASKVLLPALNVMDISDNKLLQAPPDVARLTLLSMLNLSGNTAIKELPPDYGMLSRLWSLSLKGCSLKEPLESMVNVENCKTVEIVAYLKTILEESKTYHHLRLMILGSDGVGKSVIWDALCKEAVQKRQPIHSETGVIRQAEWKFEAKRSKGDKNLGPVGFSVIDFGGQREYHSTHQYFLSKRSLNLVLWKITDGDEALAQLDTWLVNIHARAPNSTVILVGTNLDQVASNSSKFGPGYIDIMEQKVRTRYMVADADKSGLPRIVDVILINSTSRNDVKALLNTIYRTAWEVRMGKERAMEQQIPSSYIALMKVTKELGVEFRKEGQPAVMTVEAYRERVKKRMISKFGRPFRDDIEFYAACTFLHDCGELVRFEDATLRDLIFVDPLWLAEFLTSVVILRSPNLPAGLLSTDAINPHTRSFKSGALLMLKTQLLDLLHKFELALATQPRQLLIPSLLPDEYRLRSDFLASAVKIRMKMSQWNVRCPSPAGSPTKSPLRRTSPTDQNGVGSEDVMLQFTYDDDQLLRRIYALAYIPSGFWSRLVTRIVGDKNVCAAIESIFMTTSADRAKIADIATKHAKAEWVVWQTGIELHVKGHSLFTLKQFLPLAEVRDIDYSAIDMRAKDEQKRWRTWNQPSHRPIVEMVVNSLSISAASQHGRKLSMKTDVEGRSRLLAMISDLLDTLLEDWYPALGTRFVHSSEGDLLVSRYVLCPQCVRDAERNGSRSRTSSSASHRRSQDDGELPITSSSHMKGSRTTGDISKCRGGVVHCFVIEECMLAGREYNWVECPSHGGLHMRELAPDTVFADIENALTIHPDQLKRSRMLGRGAFGFVFRATVRQPNGELCEVAQKMLEPVDPGPGGRPSALAAYKAAADKWKRDSMEFACRAYCTSRQELSLLSRMKHPNVIGLVGVCTFPLSLVVELAPLGALNQLLGSHRKAGTKLSLGVIKESAVQVARALEYLHSAHIIYRDLKSENVLGWRFPAPFSPQTDVLLKLGDYGISRSVLPSGGAKGFGGTEGFMAPEIVRFNGEEEYTQKVDCFSFGMFLYELLTLKFPFESEEHVKERMLDGARPVLLPHELLLPTPMLDLLVHCWSAHPESRPSSSQLVGFCAAPEFTHLLDVCELGEALPPTQLMAVGITDEIDDPDDFEAQLWLSGREMVVMGCTQYGFVDQKSIELPHRGKYVSKVRDSVWSCDECGQVTVYGISLHETGHLQLPSLNGTLICAPELISNDVLILISDKQIVLLKLSESNSVSHLGTIDSPYEIRTATFLGNGSTRQIWAGHSEGRISIHHIASNDSFSFSSSLYLPDDKCIVRQLVGSKDAQKVWIALEKSSKVQMVEVEKRQVTGSLDIRKVMPGSETIHTIDMEMASQNYVTCIGLLERNDGDQLYIGTSKGLLVIAHATTLQPLSACRPFEGDITSICILEEPSREEENTRGKATTLSTASSESGLGWVRERVSETVDRFRSSPATVETQGAALVVCIGRQFRSLSHRFVAEEKLADVYSIAVWRTEEWAL

Target ORF information:

RefSeq Version	NM_060438.7
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Leucine-rich repeat serine/threonine-protein kinase 1 (lrk-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060438.7

ORF Insert Sequence:

ATGGACCTCT CAAGTGGGGG TCCGTCATCT TCAAGCGATG TAGCTTCAGA ATTGGATAAT 
TCGGATGCAA TGCAACTGGT TAGACAGGCA GTATTATTCG AGAACGTGGA GCTGCTGGCC 
GACTTGTTCA AAGTGAATCC TTGGGTATGG AATCGGGTTG ATCGACATGG AAGGACACCG 
TTAATGTTAG CTGCTCATAA TGGGAAGCTG GATAGTTTGA GGACTATTTT AATGCTTAGT 
CCTAATTCTC TAAATCTTGT GAACGATCGT GGTAAGACCG CACTTCATAT GGCAGCCGAA 
TCAGGTGAAA CTTCGATTGT TTTGGAGCTT GTCGAACTGG GCTCCGATCC AATGAAATCT 
GATAATGAAG GACATTGTGC ATTGGAGTTA GCTCAAATGG CTGGACATAA TGAGGTTGCC 
GCAAAGCTTA TTGATGCTAT TCAAAAAGAA AGTGAAGATC TAAATGAAGC CCACACAATG 
ATCATCAGTG CATGCATATC TGGAAGTGCT GACGTTGTTT ATGAAATTTC AAGGAGATTT 
ATGGAAAAAA AACAAAGCCG CGAAATATTA TTCAACGGTC GAAATGAAGA GGATGAGACT 
GCACTCTTGA TTGCCTGCAC AAACGGTCAT ATCGAAATCG TCCGTCACTT ATTACAATTC 
GAAGAGCATC TTCTACAAAG CCACGTATCC AAGGATACAG TAATCCATGC GGCAGTGTCG 
TCACAGAATG TGGAAGTGTT GCAGCTGTGT TTGGAGAAAT TCCCTCAACT CGTCAAATCT 
ACCAACAACG AGGGCTCTAC GTGTCTACAT TGGGCCGCCC GTTGTGGATC TTCGGAATGT 
GTTTCCACAA TTCTAAACTT TCCATTCCCA TCTGAATTTA TAATCGAAAT CGACACAGTT 
GGAGCACCAG CTTATCAGCT TGCTCTAGAC GTGAATGAAG TTGACGGAGA GTGTCGAACG 
GCAATGTATC TAGCGGTTGC CGAAGGACAT TTGGAAGTTG TCAAGGCGAT GACTGATTTC 
AAATGCACAT CAATCGACGG AAGACAACGT TGCCCGTTTC AACTTGACGT CTACTGTACT 
CGAGGACGAA CACCATTCAT GTTGGCCGCT TTTAATCAGA ATCTTCCATT GATGACGCTT 
CTTCTCGACG CTGGTGCAGA TGTCAACTTG CCGCTGGCAG TCTTGGACAC AGAGTACAGC 
GTAGAAGAAG GTCGATGCAT TGGCTCGGGT GCTCTCGTTG AAGCTGTACG ATCTGATGGA 
CTACACATTG TGCACTTTCT GTTAGACCGT GGAGCTCTGG ACACTGATAA CAAGGCTCTT 
CGACTTGCTG CTCAGGGGAA GAACGAGAAG CTCATTCGGG TTTTTCTGGT TCGCCTGGTT 
TTTGCGGACC CTGAATATAA GATCAATAAG AAAAACATTG ATGTAGGTCA GATTCAAGTT 
GGTCAGAGTC TGCTACCAAG TTCACTGTGC CCATCAAAAG CCGCCCAACT GAATTGGAAT 
TCAGCCAATT TGGAGCAACT TCAATCGGAT TGGTTTGTGG CGGCAGCTCT ACACGTGAAC 
CCGAGACTCC GAACAACGAG GTTATCACTG GCAGCAATCA CTCGGGTCGA TTTATCGGAC 
AATCGTCTCA ACACATTCCC AAGTATTCTA TTCCAAATGC CATCGCTCAG ATCTCTAAAT 
CTAGCTGATA ATAGTATACG TAAAATTGAA ATTCCAACTT ATTACATTAG TTCAACATCG 
CTAGAGATTC TCAATTTACG GAATAATCAA CTCGAGTGTA TAGCCATTCA ATTTCTATCA 
TCTCTTCCAC AATTGCAACA ATTGGATGTA TCGAAGAACG AGTTGAGCCA ACTTCCCGAG 
TATATATGGT TGTGTCCAGC GTTGAAAGAG CTCAATGCAT CCTATAATAG ACTGAGCACA 
CTTCCGATGG TTGCAAGAGC ATCAAGAGGA GAGAGACCAC GACTCAATAA TTCTAACAAC 
AATTTCAACA CCCAATCTCC AACACAAGAA TCGAATCCAA TCGTCGTTGA TGATCCTCCG 
AATGTCACTT CAAACCCTCT GCGTCGCCAA AATGTGTGGC AAGCGAGTAT CAATTTGTCA 
AAAGTCGACG ACGATTCCCT GTTCCCCGAC TTCCCGGTCA CTTCCAGTAA CACATTGACG 
ACGATAAATC TATCTTTCAA TAAATTCCAC ACATTCCCAT TCTGCCTGGC ATGCACATGT 
CCCCGGCTTC TGATTCTAAA CATGTCAAAC AACTCAATGA CGTCACTTCC ACCTATGGCT 
TGTGTACCTG CGCATCTGAG GACGTTGGAT CTGTCTTACA ATAAGATACA AGAAAGTTTC 
ATCGAAGCTT CACCTCTCCA CGTGGTCTGT CACGCGGTCC CACCGACAAC GTCTAATGGC 
TCAATGCTCC CAAAGCGAAG AAATTCTCCA GCTCGGCAAC ATAGAAGTCG TTCGAAGTCT 
GCAGTTAGAT CACAGAGAAG TCTCAGTGTT TCTCGCCATC ATGCGCTAAT TGATCCACAA 
AAAGAAGAAG AAAGTTGTGT TCATAAGCGT CACGATTCAT TAGAATGGCT CAAGACACTA 
CAATTGGCTG GAAATCGACT CCGATCAATT TCAGTGACAA ATGCAGCTTC AAAAGTTCTC 
CTTCCAGCTC TGAACGTGAT GGATATCAGT GACAACAAAT TGCTACAAGC ACCACCGGAT 
GTTGCACGGC TCACACTTCT TTCAATGCTC AACTTATCAG GAAATACTGC AATTAAAGAA 
CTACCACCAG ATTATGGAAT GCTCTCTCGT CTTTGGAGTC TATCTTTGAA AGGATGCTCA 
CTGAAAGAAC CACTTGAATC AATGGTTAAT GTGGAGAACT GCAAGACAGT GGAAATTGTG 
GCGTATTTGA AGACAATTCT AGAAGAATCC AAGACATACC ATCATTTGAG ATTAATGATT 
TTGGGAAGTG ATGGCGTTGG AAAATCTGTT ATTTGGGATG CACTGTGCAA GGAAGCAGTA 
CAGAAACGGC AGCCTATTCA CTCGGAAACT GGTGTTATTC GGCAAGCTGA ATGGAAGTTC 
GAAGCGAAAC GATCTAAAGG CGATAAGAAT CTTGGGCCTG TCGGATTTTC TGTAATCGAC 
TTTGGAGGTC AACGAGAATA TCATTCCACT CATCAATATT TCCTGAGCAA ACGATCCTTG 
AACTTGGTTC TTTGGAAGAT CACAGACGGG GATGAAGCAC TGGCGCAGCT TGATACTTGG 
CTAGTTAATA TCCATGCAAG AGCGCCAAAC TCGACAGTAA TACTAGTGGG AACCAATTTG 
GATCAAGTGG CATCGAATAG TTCGAAATTT GGACCTGGAT ACATAGACAT CATGGAGCAA 
AAAGTGCGGA CCCGTTATAT GGTTGCAGAT GCGGATAAGT CAGGATTGCC ACGTATTGTT 
GATGTAATTT TGATCAATTC AACGTCAAGA AATGATGTAA AAGCACTGCT AAACACAATA 
TATAGAACTG CGTGGGAAGT ACGAATGGGA AAAGAAAGAG CTATGGAACA ACAGATTCCA 
TCTTCTTACA TTGCTTTGAT GAAAGTAACA AAAGAATTAG GCGTCGAGTT TCGGAAAGAG 
GGGCAGCCGG CTGTAATGAC TGTAGAGGCA TATAGAGAAA GAGTCAAGAA AAGGATGATT 
AGCAAGTTTG GCAGACCGTT TAGGGACGAC ATTGAATTCT ACGCCGCATG CACATTCCTT 
CACGATTGTG GTGAATTAGT TCGATTTGAG GATGCCACTC TTCGGGATCT GATTTTTGTA 
GATCCGCTGT GGCTCGCCGA GTTTCTCACT TCTGTCGTCA TTCTAAGAAG CCCAAATCTT 
CCAGCTGGAC TTCTTTCAAC AGACGCTATA AATCCGCATA CTCGTTCATT CAAATCAGGA 
GCACTTTTAA TGCTCAAAAC ACAGCTTTTG GATCTTCTTC ACAAATTCGA ATTGGCACTT 
GCTACCCAGC CTCGACAGCT TCTGATTCCA AGCCTGCTAC CAGATGAGTA CAGGCTACGA 
TCTGATTTCT TAGCATCTGC AGTTAAAATC CGAATGAAAA TGTCACAGTG GAATGTCAGA 
TGTCCAAGTC CCGCTGGATC ACCGACCAAA TCCCCACTCC GTCGAACATC ACCTACGGAT 
CAGAACGGTG TTGGATCAGA GGATGTTATG CTTCAATTCA CTTATGACGA TGATCAATTG 
TTAAGAAGGA TTTATGCGTT GGCTTATATA CCTTCAGGAT TTTGGAGTCG CCTAGTGACT 
CGTATAGTTG GAGATAAAAA CGTGTGTGCA GCAATTGAAA GTATTTTCAT GACAACAAGT 
GCGGATCGAG CAAAGATTGC AGATATAGCC ACTAAGCATG CAAAAGCTGA ATGGGTTGTG 
TGGCAAACTG GAATAGAGCT TCATGTTAAA GGACATTCAC TTTTCACTCT GAAACAGTTT 
CTACCGTTGG CAGAGGTTAG AGACATTGAC TACAGCGCCA TAGACATGCG CGCAAAGGAT 
GAACAAAAAC GATGGAGGAC ATGGAATCAA CCTTCACACC GCCCAATTGT CGAAATGGTT 
GTTAACTCAT TGAGCATTTC AGCAGCTTCA CAACACGGAA GAAAACTTTC AATGAAAACA 
GATGTGGAAG GAAGAAGCCG ATTATTGGCA ATGATATCAG ATTTATTGGA CACGTTACTC 
GAAGACTGGT ATCCGGCTCT TGGAACTCGA TTTGTTCACA GTTCGGAAGG CGACTTGCTT 
GTTTCACGAT ATGTGCTTTG TCCGCAGTGT GTCAGGGATG CTGAGAGAAA TGGCTCACGG 
AGCCGAACTT CTTCTTCAGC GTCACACCGA AGATCACAGG ATGACGGAGA GCTTCCAATA 
ACCAGCAGCT CTCACATGAA AGGTTCTCGA ACAACAGGAG ACATTTCAAA ATGTCGCGGA 
GGAGTTGTTC ACTGTTTCGT TATTGAGGAA TGTATGCTGG CTGGAAGAGA GTACAACTGG 
GTAGAATGCC CATCTCATGG TGGTCTTCAT ATGAGAGAAC TTGCACCGGA TACTGTATTT 
GCTGATATCG AAAATGCTTT GACAATTCAC CCGGATCAGT TAAAACGAAG CAGAATGCTC 
GGAAGAGGAG CGTTTGGTTT CGTTTTTCGG GCTACAGTAA GACAACCAAA TGGAGAACTT 
TGTGAAGTTG CTCAGAAAAT GTTGGAGCCT GTAGACCCAG GGCCTGGAGG CAGGCCTTCG 
GCTCTCGCAG CTTATAAGGC AGCAGCAGAC AAATGGAAGA GGGATAGTAT GGAATTTGCG 
TGTAGAGCAT ACTGTACTTC AAGACAAGAG CTCAGTTTAT TGAGTAGGAT GAAACATCCA 
AATGTTATTG GACTAGTTGG AGTTTGCACT TTTCCATTGT CTCTTGTTGT AGAGCTTGCA 
CCTCTCGGAG CACTTAACCA GCTGCTGGGA TCTCATCGAA AAGCTGGAAC TAAGCTCTCA 
CTTGGAGTTA TCAAAGAGTC TGCAGTTCAA GTTGCTCGAG CACTGGAATA TCTTCATTCT 
GCACATATTA TCTACAGAGA TCTCAAGTCG GAAAATGTAC TTGGATGGAG ATTTCCTGCT 
CCATTCAGTC CACAGACTGA TGTTCTATTG AAACTTGGAG ATTACGGGAT ATCAAGAAGT 
GTACTTCCAT CAGGTGGAGC AAAGGGATTC GGAGGAACGG AAGGATTTAT GGCTCCAGAA 
ATTGTAAGGT TCAATGGAGA AGAGGAATAT ACACAAAAAG TGGATTGCTT TTCGTTTGGA 
ATGTTCCTCT ATGAACTTCT CACTCTGAAA TTCCCATTCG AAAGTGAAGA GCATGTGAAA 
GAAAGAATGC TCGACGGAGC CCGTCCCGTA CTTTTACCGC ACGAGCTTCT CCTTCCAACT 
CCAATGCTGG ATCTTCTCGT TCACTGCTGG TCGGCTCATC CAGAATCCCG ACCTTCGTCG 
TCTCAACTAG TCGGATTCTG TGCAGCTCCA GAATTCACAC ATCTTCTTGA TGTTTGTGAA 
CTTGGAGAAG CTCTACCACC AACTCAGCTG ATGGCAGTAG GAATAACGGA CGAAATTGAT 
GATCCGGATG ACTTTGAAGC TCAACTTTGG CTCAGTGGAC GGGAAATGGT AGTCATGGGA 
TGCACACAAT ACGGATTTGT TGACCAAAAA TCGATTGAGT TACCTCATCG AGGCAAGTAT 
GTGAGCAAGG TTCGAGATTC TGTGTGGTCT TGCGACGAAT GTGGACAGGT TACTGTATAT 
GGAATATCTC TACATGAAAC CGGGCACTTG CAGTTGCCAT CACTTAATGG AACTTTGATT 
TGTGCACCTG AACTGATAAG CAACGACGTG CTTATACTAA TTTCTGATAA ACAAATTGTG 
CTTTTGAAGC TTTCCGAATC AAATTCCGTA TCCCATCTTG GAACAATCGA TTCACCGTAT 
GAAATTCGGA CAGCCACATT CCTGGGCAAT GGTTCAACCC GTCAAATATG GGCGGGACAT 
TCAGAAGGAC GAATTTCAAT TCATCATATA GCTTCGAACG ATTCTTTCAG CTTCTCGTCA 
AGTCTTTATC TTCCAGATGA TAAGTGTATT GTCAGACAGT TAGTTGGCTC GAAAGATGCA 
CAAAAAGTGT GGATCGCATT AGAGAAAAGC TCGAAAGTTC AAATGGTTGA AGTGGAAAAA 
CGACAAGTGA CTGGATCACT GGATATTCGA AAAGTGATGC CTGGAAGTGA AACAATTCAT 
ACAATTGATA TGGAAATGGC CAGTCAGAAT TATGTTACAT GTATTGGTTT ATTGGAAAGA 
AATGACGGAG ATCAACTGTA CATTGGAACT TCCAAAGGGC TGCTAGTCAT AGCTCACGCT 
ACTACACTTC AACCATTGTC AGCATGCCGT CCATTTGAAG GAGACATCAC ATCAATTTGT 
ATATTGGAAG AACCATCCCG TGAAGAAGAA AATACACGTG GAAAGGCTAC CACATTGAGC 
ACAGCTTCAT CAGAAAGTGG TCTTGGATGG GTACGGGAAA GAGTCAGCGA AACTGTTGAT 
AGATTCCGCT CGAGCCCAGC TACAGTAGAA ACTCAGGGAG CCGCTCTTGT TGTGTGCATT 
GGACGACAAT TCAGAAGCCT TTCACATCGT TTCGTGGCTG AGGAGAAGCT TGCCGACGTC 
TATTCCATTG CTGTCTGGAG GACCGAGGAA TGGGCTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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lrk-1 cDNA ORF clone, Caenorhabditis elegans

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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