aspm-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	aspm-1
Entrez Gene ID	172746
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_060066.3	NP_492467.1	hypothetical protein
NM_060066.4	NP_492467.1	Calponin-homology (CH) domain-containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mei-1 and aspm-1 promote oocyte meiotic spindle pole assembly
Title: Caenorhabditis elegans oocyte meiotic spindle pole assembly requires microtubule severing and the calponin homology domain protein ASPM-1.

ASPM-1, together with calmodulin (CMD-1), promotes meiotic spindle organization and the accumulation of LIN-5 at meiotic and mitotic spindle poles.
Title: NuMA-related LIN-5, ASPM-1, calmodulin and dynein promote meiotic spindle rotation independently of cortical LIN-5/GPR/Galpha.

The following aspm-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aspm-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa350860	NM_060066.4 Latest version!	Caenorhabditis elegans Calponin-homology (CH) domain-containing protein (aspm-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa350860	NM_060066.3	Caenorhabditis elegans Uncharacterized protein (aspm-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

aspm-1 ( NM_060066.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492467.1 Caenorhabditis elegans Uncharacterized protein (aspm-1), partial mRNA.

Price & Availability↑

CloneID	OCa350860
Clone ID Related Accession (Same CDS sequence)	NM_060066.3 , NM_060066.4
Accession Version	NM_060066.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-09
Organism	Caenorhabditis elegans
Product	hypothetical protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_060066.2. COMPLETENESS: incomplete on both ends.

ATGGATAATA ACGTTGAGGA TAAAAAAGAG GCAATGCTCA AACGATTAGC AAATCGACAC 
GAGAAACGCC TTCTTGATCA AGTCAAATCG AACACAAAGA AGATCGATTT GAGAGCAACC 
GAGCGAGCCT TTTTGGAGTC GAGTCCGACT TCAATGAATA TGCGAACTCC TCTCAATCCT 
TCAATCAGCT CTTCTGTATC AGATTCCCCG ATTCTATCAT ATGATGAGAA AGCCAACAAG 
CAAATTATTG CACTCGCCAC GTGGTGCAAC ACAATGATGG AATTGGATGT GTCTGAAGAG 
ATGGATCTAG GAGAGTCGAA AGCAGAAGCC TGCAGAAATA TCCAAAAAAT GTTGAAGAAA 
AGATCAGATA CGTCAGAAGT GGAAAATACA CAGGAAAATG CACGCAGGAG ATATCAACGG 
ATTTTTGAGA AAAATGATCC AGAAGTTGTG AAGAAAAAGT GCAAGCAGCT TCTAGACGAT 
TCAGGAATGG ATGCAAGCAT CAAGGATCTT CTGTCCAAAA ACAACGTCGC AATCCGAAAA 
GAACATGCCG TATATAATGA CATAGGCCTC CAAACAACTC TTCTTCACAC ATTTCTATCA 
TTCCATCCTG CTTGGCTGAA AACTGCTCTT GAAGCAATCT TTAACACGAG AATTGACGCT 
CAACCGAAGC ATTTAATGAA AAAACTTTCT CAGTTTTTCC TCGATCTAGT TTTCTCAAAT 
CCGACGATGC TAAAGAACAA GAAATTTGCA CAAGGTTCTG GAAAACCGAT CATCACTGAG 
GCTGGCAAAG AAGCTCTACA CAAGCATTTT CTGTCCGTCT CGATGAAGCT GATGTTCTTG 
ATTGAAACGG CACACACTCA TCGAGTTATC CCGAATCTGA CGAGAATCTT CACGAAGTCC 
TCTCATTTCA ATTGTCTCGA CGATGTGTTC TCCGAACTGA CGAAGGAATT GTTGACTGGA 
AGCTCTGCTA CTTTCAAAAA AGCGTTTGCA AAAGTCGGTT TTATTCCGAC TTACCGACAA 
TCATTCATTG AAAATTACGA CTACCAAGCG AAAGGATTTT CAGATTTCTC GGATGGTCTA 
ATTTTGGCAA AGCTGTTGGA AACTGTCGGA GAGATGCCGC ATGGTCAAAT TCTGTTGAAG 
TTACGTGATC CAGCAGGCGA CCGCATCCGC AAAATTGGAA ACGTGAAGAT TGTACTCCAG 
GAGATGTCTT CTTTGGGTGT TCCAACGGAT AATGTTAACG CCGAATCAAT CGTTGGCGGA 
AAGAAAGACG AGATTTTGTC TATTTTGTGG GCTATTATCG GAGTTCGTGT GGCCAAGGAA 
CAAAGAATCA AGGTGACGAG AGTTTCTGAA GAAAGAACTC CGAAAAAGCG GCGATCTGCT 
GTCCACGATG ACATGTCTTC TGAAGTATTA AAAATGTGCA AGATTTACGG AAGACAGATG 
GAAATCGAAG TCATGGACTT GGATTCACTC TCCGATGGGT GCCTTCTGGC CAAGTTGTGG 
ACCACTTTCG GAACAAATTC AACACCGATT CAGGACTATG ATGGACTCAG TCTGTGGGAG 
AAAGTTGTTT CAGTTGCCGA GTTGGAGCTT TGTATTCAGC GAGGACTTGA TCAGAACATG 
GCTCTTTTCG TAAAAATGTT TCTGGAACGA CTGGGTATGA TTCAAGATCT AAATGAGAAA 
GCGACCAAGA TTCAACGAAT GTGGAAGGCC TACGTGCAAA GAAAGAATAC TCCCAAGCTG 
TATTTCATTG TACAACAACT TCTTGCCGAC TCATCAATTC CTCGAAACCG AAGTGTTTCT 
CCATTCTCTA ATAATGTCAC TTTCACAGTA CCGAGGACTC CACGGAATAA TATTCTGACT 
GAAAGACCAT CTCTATCACA AATCCCATCG TCTCGCCAAT CAATGGATTC TACCTTCAAC 
GATGCGACGT TTACTGTTTC ACGTGATTCA ATTGAAAGCA TGAACAAGAT GCAGAAAACT 
CCACTGAGAG GTACATTCAC AAGAAAAACA ATTGCGATGG TTATCGAGGA AGAGGATGAT 
TCGGAAAATA ACGAAACGGT CGTACCAAGT ACATTGAAAA AGAGAACCGT TGTCAGAATG 
GAGCACAATG CGGAAGTATT CAGAGAACAA GATGAAGATG ATGAAAACCA AGACAAAGAC 
ACTGTTGCTC CTTCCGCTGA GAACTTGGAT AGTCCACCTT CAGACATCCC ATTAGAAACT 
CTTTCTTCTA TTCCTTCCGC TTCCCAATCT GCCATATTTC TTCAAGACTC TGAAACTGGC 
AAAGAGATGC ATGTCCCTAA AGCTGAAGAT GTCGGCGTCG TAGTTCTTGA AGCATCAGAT 
TCTCCGGTTG CACTAGAAGG GAACAATGAA GCAAGCTATG ATGGTCAGAA AATTGAGAAC 
TTAGAAACTT TTGAAATCAA AGAGGGCAAG ACGCAAGAAG ATCTACCTTC AAAATCTCCT 
ATGGATCCTA CGCAGACAAG CGGAAGCCCA TTGGTTGAAT TCAGAATGAC AGAAGAACAG 
GAGCGTTTAG AGATGCTTTT CCAGTCATTA TCAGAAGATC AGAAGAACTT CGTCAAGACG 
AATAACCTTA GTGTGTCTAT TGAGGACGAT GCCAACACTC CTGAACTTAG AAGGATCTTG 
AGACAAACCA GAGAACTCAA GAGAAAGCAA CAGGAAATTG CGAGAAAACT GGGAAACATC 
GAGAGAAATG CGTTAGCTGT TCGTGATGGC GGGGAGGATT CGTCCGACAG TAGATCAGAT 
GCTGGGCACG ATGTTGCTAT CCTTCACGGT GACGACAGTC AACTCTTCGA AAATTCAATG 
CAATTGGATC AGAAATCTCA ATTGCAAAAT GATGAGACTC AAATTCTTGA GAATAAAAAG 
AAGGCAGCTG TTGTCATTCA GAAAATGATA CGTGGTTTCA TTGCACGGCG TAAATTCCAA 
ATGGAAATTA GCAATATTCG CAATCGCATG ATCCAATACA ATCACATATT GGCGCAAGAA 
GACGAGCAAA TTGGTATAGA AGAAATGGAG GATAAATCAG TCGAGGCAAA GCTAAAGAAA 
TGCGCTCTTC ATGGACTCAC AAATGATAAC CTTCACGTCG TTCATGTTGC TGCAACTGTC 
ATCGACCGAG TCACCGATCT TGTTCCATCA CTTTTGGAAA AGCTTGTCGT CGAACTGAAT 
GGCATCAAGT CGATCACTGA TGTTTTGATT GTGGCGGATC GAGGATTCTC TTATACGAGC 
ATTCTTCATC CATTATTGAA TGTTCTCGAA AAATTATTCA CGAAGATTCC CGAAGAAGTC 
ACCAATTCGC ATATTCTCCC TCACTTGAAG ATTTTGTCGC CTCGCTTGAT GCTACTGATG 
CTGAAGCACT CGGAAGTCAA GGAATTGTTT ATTCCTATCA TTAACACCTT GACTTCAATT 
GCTAGAAGAT TCCCAGACAA AAAGCAATCG AGTTTTTCTT CATCAAGCTA CTCAATCAAG 
CAGTCGCTCA AAAAAGTCAA GGATCAGGAG TGTCGGAATA AATTGGTCAG ATTGGAAAGC 
ATATGCGAAG ATATGCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492467.1
CDS	1..3561
Translation	MDNNVEDKKEAMLKRLANRHEKRLLDQVKSNTKKIDLRATERAFLESSPTSMNMRTPLNPSISSSVSDSPILSYDEKANKQIIALATWCNTMMELDVSEEMDLGESKAEACRNIQKMLKKRSDTSEVENTQENARRRYQRIFEKNDPEVVKKKCKQLLDDSGMDASIKDLLSKNNVAIRKEHAVYNDIGLQTTLLHTFLSFHPAWLKTALEAIFNTRIDAQPKHLMKKLSQFFLDLVFSNPTMLKNKKFAQGSGKPIITEAGKEALHKHFLSVSMKLMFLIETAHTHRVIPNLTRIFTKSSHFNCLDDVFSELTKELLTGSSATFKKAFAKVGFIPTYRQSFIENYDYQAKGFSDFSDGLILAKLLETVGEMPHGQILLKLRDPAGDRIRKIGNVKIVLQEMSSLGVPTDNVNAESIVGGKKDEILSILWAIIGVRVAKEQRIKVTRVSEERTPKKRRSAVHDDMSSEVLKMCKIYGRQMEIEVMDLDSLSDGCLLAKLWTTFGTNSTPIQDYDGLSLWEKVVSVAELELCIQRGLDQNMALFVKMFLERLGMIQDLNEKATKIQRMWKAYVQRKNTPKLYFIVQQLLADSSIPRNRSVSPFSNNVTFTVPRTPRNNILTERPSLSQIPSSRQSMDSTFNDATFTVSRDSIESMNKMQKTPLRGTFTRKTIAMVIEEEDDSENNETVVPSTLKKRTVVRMEHNAEVFREQDEDDENQDKDTVAPSAENLDSPPSDIPLETLSSIPSASQSAIFLQDSETGKEMHVPKAEDVGVVVLEASDSPVALEGNNEASYDGQKIENLETFEIKEGKTQEDLPSKSPMDPTQTSGSPLVEFRMTEEQERLEMLFQSLSEDQKNFVKTNNLSVSIEDDANTPELRRILRQTRELKRKQQEIARKLGNIERNALAVRDGGEDSSDSRSDAGHDVAILHGDDSQLFENSMQLDQKSQLQNDETQILENKKKAAVVIQKMIRGFIARRKFQMEISNIRNRMIQYNHILAQEDEQIGIEEMEDKSVEAKLKKCALHGLTNDNLHVVHVAATVIDRVTDLVPSLLEKLVVELNGIKSITDVLIVADRGFSYTSILHPLLNVLEKLFTKIPEEVTNSHILPHLKILSPRLMLLMLKHSEVKELFIPIINTLTSIARRFPDKKQSSFSSSSYSIKQSLKKVKDQECRNKLVRLESICEDML

Target ORF information:

RefSeq Version	NM_060066.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (aspm-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060066.3

ORF Insert Sequence:

ATGGATAATA ACGTTGAGGA TAAAAAAGAG GCAATGCTCA AACGATTAGC AAATCGACAC 
GAGAAACGCC TTCTTGATCA AGTCAAATCG AACACAAAGA AGATCGATTT GAGAGCAACC 
GAGCGAGCCT TTTTGGAGTC GAGTCCGACT TCAATGAATA TGCGAACTCC TCTCAATCCT 
TCAATCAGCT CTTCTGTATC AGATTCCCCG ATTCTATCAT ATGATGAGAA AGCCAACAAG 
CAAATTATTG CACTCGCCAC GTGGTGCAAC ACAATGATGG AATTGGATGT GTCTGAAGAG 
ATGGATCTAG GAGAGTCGAA AGCAGAAGCC TGCAGAAATA TCCAAAAAAT GTTGAAGAAA 
AGATCAGATA CGTCAGAAGT GGAAAATACA CAGGAAAATG CACGCAGGAG ATATCAACGG 
ATTTTTGAGA AAAATGATCC AGAAGTTGTG AAGAAAAAGT GCAAGCAGCT TCTAGACGAT 
TCAGGAATGG ATGCAAGCAT CAAGGATCTT CTGTCCAAAA ACAACGTCGC AATCCGAAAA 
GAACATGCCG TATATAATGA CATAGGCCTC CAAACAACTC TTCTTCACAC ATTTCTATCA 
TTCCATCCTG CTTGGCTGAA AACTGCTCTT GAAGCAATCT TTAACACGAG AATTGACGCT 
CAACCGAAGC ATTTAATGAA AAAACTTTCT CAGTTTTTCC TCGATCTAGT TTTCTCAAAT 
CCGACGATGC TAAAGAACAA GAAATTTGCA CAAGGTTCTG GAAAACCGAT CATCACTGAG 
GCTGGCAAAG AAGCTCTACA CAAGCATTTT CTGTCCGTCT CGATGAAGCT GATGTTCTTG 
ATTGAAACGG CACACACTCA TCGAGTTATC CCGAATCTGA CGAGAATCTT CACGAAGTCC 
TCTCATTTCA ATTGTCTCGA CGATGTGTTC TCCGAACTGA CGAAGGAATT GTTGACTGGA 
AGCTCTGCTA CTTTCAAAAA AGCGTTTGCA AAAGTCGGTT TTATTCCGAC TTACCGACAA 
TCATTCATTG AAAATTACGA CTACCAAGCG AAAGGATTTT CAGATTTCTC GGATGGTCTA 
ATTTTGGCAA AGCTGTTGGA AACTGTCGGA GAGATGCCGC ATGGTCAAAT TCTGTTGAAG 
TTACGTGATC CAGCAGGCGA CCGCATCCGC AAAATTGGAA ACGTGAAGAT TGTACTCCAG 
GAGATGTCTT CTTTGGGTGT TCCAACGGAT AATGTTAACG CCGAATCAAT CGTTGGCGGA 
AAGAAAGACG AGATTTTGTC TATTTTGTGG GCTATTATCG GAGTTCGTGT GGCCAAGGAA 
CAAAGAATCA AGGTGACGAG AGTTTCTGAA GAAAGAACTC CGAAAAAGCG GCGATCTGCT 
GTCCACGATG ACATGTCTTC TGAAGTATTA AAAATGTGCA AGATTTACGG AAGACAGATG 
GAAATCGAAG TCATGGACTT GGATTCACTC TCCGATGGGT GCCTTCTGGC CAAGTTGTGG 
ACCACTTTCG GAACAAATTC AACACCGATT CAGGACTATG ATGGACTCAG TCTGTGGGAG 
AAAGTTGTTT CAGTTGCCGA GTTGGAGCTT TGTATTCAGC GAGGACTTGA TCAGAACATG 
GCTCTTTTCG TAAAAATGTT TCTGGAACGA CTGGGTATGA TTCAAGATCT AAATGAGAAA 
GCGACCAAGA TTCAACGAAT GTGGAAGGCC TACGTGCAAA GAAAGAATAC TCCCAAGCTG 
TATTTCATTG TACAACAACT TCTTGCCGAC TCATCAATTC CTCGAAACCG AAGTGTTTCT 
CCATTCTCTA ATAATGTCAC TTTCACAGTA CCGAGGACTC CACGGAATAA TATTCTGACT 
GAAAGACCAT CTCTATCACA AATCCCATCG TCTCGCCAAT CAATGGATTC TACCTTCAAC 
GATGCGACGT TTACTGTTTC ACGTGATTCA ATTGAAAGCA TGAACAAGAT GCAGAAAACT 
CCACTGAGAG GTACATTCAC AAGAAAAACA ATTGCGATGG TTATCGAGGA AGAGGATGAT 
TCGGAAAATA ACGAAACGGT CGTACCAAGT ACATTGAAAA AGAGAACCGT TGTCAGAATG 
GAGCACAATG CGGAAGTATT CAGAGAACAA GATGAAGATG ATGAAAACCA AGACAAAGAC 
ACTGTTGCTC CTTCCGCTGA GAACTTGGAT AGTCCACCTT CAGACATCCC ATTAGAAACT 
CTTTCTTCTA TTCCTTCCGC TTCCCAATCT GCCATATTTC TTCAAGACTC TGAAACTGGC 
AAAGAGATGC ATGTCCCTAA AGCTGAAGAT GTCGGCGTCG TAGTTCTTGA AGCATCAGAT 
TCTCCGGTTG CACTAGAAGG GAACAATGAA GCAAGCTATG ATGGTCAGAA AATTGAGAAC 
TTAGAAACTT TTGAAATCAA AGAGGGCAAG ACGCAAGAAG ATCTACCTTC AAAATCTCCT 
ATGGATCCTA CGCAGACAAG CGGAAGCCCA TTGGTTGAAT TCAGAATGAC AGAAGAACAG 
GAGCGTTTAG AGATGCTTTT CCAGTCATTA TCAGAAGATC AGAAGAACTT CGTCAAGACG 
AATAACCTTA GTGTGTCTAT TGAGGACGAT GCCAACACTC CTGAACTTAG AAGGATCTTG 
AGACAAACCA GAGAACTCAA GAGAAAGCAA CAGGAAATTG CGAGAAAACT GGGAAACATC 
GAGAGAAATG CGTTAGCTGT TCGTGATGGC GGGGAGGATT CGTCCGACAG TAGATCAGAT 
GCTGGGCACG ATGTTGCTAT CCTTCACGGT GACGACAGTC AACTCTTCGA AAATTCAATG 
CAATTGGATC AGAAATCTCA ATTGCAAAAT GATGAGACTC AAATTCTTGA GAATAAAAAG 
AAGGCAGCTG TTGTCATTCA GAAAATGATA CGTGGTTTCA TTGCACGGCG TAAATTCCAA 
ATGGAAATTA GCAATATTCG CAATCGCATG ATCCAATACA ATCACATATT GGCGCAAGAA 
GACGAGCAAA TTGGTATAGA AGAAATGGAG GATAAATCAG TCGAGGCAAA GCTAAAGAAA 
TGCGCTCTTC ATGGACTCAC AAATGATAAC CTTCACGTCG TTCATGTTGC TGCAACTGTC 
ATCGACCGAG TCACCGATCT TGTTCCATCA CTTTTGGAAA AGCTTGTCGT CGAACTGAAT 
GGCATCAAGT CGATCACTGA TGTTTTGATT GTGGCGGATC GAGGATTCTC TTATACGAGC 
ATTCTTCATC CATTATTGAA TGTTCTCGAA AAATTATTCA CGAAGATTCC CGAAGAAGTC 
ACCAATTCGC ATATTCTCCC TCACTTGAAG ATTTTGTCGC CTCGCTTGAT GCTACTGATG 
CTGAAGCACT CGGAAGTCAA GGAATTGTTT ATTCCTATCA TTAACACCTT GACTTCAATT 
GCTAGAAGAT TCCCAGACAA AAAGCAATCG AGTTTTTCTT CATCAAGCTA CTCAATCAAG 
CAGTCGCTCA AAAAAGTCAA GGATCAGGAG TGTCGGAATA AATTGGTCAG ATTGGAAAGC 
ATATGCGAAG ATATGCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aspm-1 ( NM_060066.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_492467.1 Caenorhabditis elegans Calponin-homology (CH) domain-containing protein (aspm-1), mRNA.

Price & Availability↑

CloneID	OCa350860
Clone ID Related Accession (Same CDS sequence)	NM_060066.3 , NM_060066.4
Accession Version	NM_060066.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Calponin-homology (CH) domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_060066.3.

ATGGATAATA ACGTTGAGGA TAAAAAAGAG GCAATGCTCA AACGATTAGC AAATCGACAC 
GAGAAACGCC TTCTTGATCA AGTCAAATCG AACACAAAGA AGATCGATTT GAGAGCAACC 
GAGCGAGCCT TTTTGGAGTC GAGTCCGACT TCAATGAATA TGCGAACTCC TCTCAATCCT 
TCAATCAGCT CTTCTGTATC AGATTCCCCG ATTCTATCAT ATGATGAGAA AGCCAACAAG 
CAAATTATTG CACTCGCCAC GTGGTGCAAC ACAATGATGG AATTGGATGT GTCTGAAGAG 
ATGGATCTAG GAGAGTCGAA AGCAGAAGCC TGCAGAAATA TCCAAAAAAT GTTGAAGAAA 
AGATCAGATA CGTCAGAAGT GGAAAATACA CAGGAAAATG CACGCAGGAG ATATCAACGG 
ATTTTTGAGA AAAATGATCC AGAAGTTGTG AAGAAAAAGT GCAAGCAGCT TCTAGACGAT 
TCAGGAATGG ATGCAAGCAT CAAGGATCTT CTGTCCAAAA ACAACGTCGC AATCCGAAAA 
GAACATGCCG TATATAATGA CATAGGCCTC CAAACAACTC TTCTTCACAC ATTTCTATCA 
TTCCATCCTG CTTGGCTGAA AACTGCTCTT GAAGCAATCT TTAACACGAG AATTGACGCT 
CAACCGAAGC ATTTAATGAA AAAACTTTCT CAGTTTTTCC TCGATCTAGT TTTCTCAAAT 
CCGACGATGC TAAAGAACAA GAAATTTGCA CAAGGTTCTG GAAAACCGAT CATCACTGAG 
GCTGGCAAAG AAGCTCTACA CAAGCATTTT CTGTCCGTCT CGATGAAGCT GATGTTCTTG 
ATTGAAACGG CACACACTCA TCGAGTTATC CCGAATCTGA CGAGAATCTT CACGAAGTCC 
TCTCATTTCA ATTGTCTCGA CGATGTGTTC TCCGAACTGA CGAAGGAATT GTTGACTGGA 
AGCTCTGCTA CTTTCAAAAA AGCGTTTGCA AAAGTCGGTT TTATTCCGAC TTACCGACAA 
TCATTCATTG AAAATTACGA CTACCAAGCG AAAGGATTTT CAGATTTCTC GGATGGTCTA 
ATTTTGGCAA AGCTGTTGGA AACTGTCGGA GAGATGCCGC ATGGTCAAAT TCTGTTGAAG 
TTACGTGATC CAGCAGGCGA CCGCATCCGC AAAATTGGAA ACGTGAAGAT TGTACTCCAG 
GAGATGTCTT CTTTGGGTGT TCCAACGGAT AATGTTAACG CCGAATCAAT CGTTGGCGGA 
AAGAAAGACG AGATTTTGTC TATTTTGTGG GCTATTATCG GAGTTCGTGT GGCCAAGGAA 
CAAAGAATCA AGGTGACGAG AGTTTCTGAA GAAAGAACTC CGAAAAAGCG GCGATCTGCT 
GTCCACGATG ACATGTCTTC TGAAGTATTA AAAATGTGCA AGATTTACGG AAGACAGATG 
GAAATCGAAG TCATGGACTT GGATTCACTC TCCGATGGGT GCCTTCTGGC CAAGTTGTGG 
ACCACTTTCG GAACAAATTC AACACCGATT CAGGACTATG ATGGACTCAG TCTGTGGGAG 
AAAGTTGTTT CAGTTGCCGA GTTGGAGCTT TGTATTCAGC GAGGACTTGA TCAGAACATG 
GCTCTTTTCG TAAAAATGTT TCTGGAACGA CTGGGTATGA TTCAAGATCT AAATGAGAAA 
GCGACCAAGA TTCAACGAAT GTGGAAGGCC TACGTGCAAA GAAAGAATAC TCCCAAGCTG 
TATTTCATTG TACAACAACT TCTTGCCGAC TCATCAATTC CTCGAAACCG AAGTGTTTCT 
CCATTCTCTA ATAATGTCAC TTTCACAGTA CCGAGGACTC CACGGAATAA TATTCTGACT 
GAAAGACCAT CTCTATCACA AATCCCATCG TCTCGCCAAT CAATGGATTC TACCTTCAAC 
GATGCGACGT TTACTGTTTC ACGTGATTCA ATTGAAAGCA TGAACAAGAT GCAGAAAACT 
CCACTGAGAG GTACATTCAC AAGAAAAACA ATTGCGATGG TTATCGAGGA AGAGGATGAT 
TCGGAAAATA ACGAAACGGT CGTACCAAGT ACATTGAAAA AGAGAACCGT TGTCAGAATG 
GAGCACAATG CGGAAGTATT CAGAGAACAA GATGAAGATG ATGAAAACCA AGACAAAGAC 
ACTGTTGCTC CTTCCGCTGA GAACTTGGAT AGTCCACCTT CAGACATCCC ATTAGAAACT 
CTTTCTTCTA TTCCTTCCGC TTCCCAATCT GCCATATTTC TTCAAGACTC TGAAACTGGC 
AAAGAGATGC ATGTCCCTAA AGCTGAAGAT GTCGGCGTCG TAGTTCTTGA AGCATCAGAT 
TCTCCGGTTG CACTAGAAGG GAACAATGAA GCAAGCTATG ATGGTCAGAA AATTGAGAAC 
TTAGAAACTT TTGAAATCAA AGAGGGCAAG ACGCAAGAAG ATCTACCTTC AAAATCTCCT 
ATGGATCCTA CGCAGACAAG CGGAAGCCCA TTGGTTGAAT TCAGAATGAC AGAAGAACAG 
GAGCGTTTAG AGATGCTTTT CCAGTCATTA TCAGAAGATC AGAAGAACTT CGTCAAGACG 
AATAACCTTA GTGTGTCTAT TGAGGACGAT GCCAACACTC CTGAACTTAG AAGGATCTTG 
AGACAAACCA GAGAACTCAA GAGAAAGCAA CAGGAAATTG CGAGAAAACT GGGAAACATC 
GAGAGAAATG CGTTAGCTGT TCGTGATGGC GGGGAGGATT CGTCCGACAG TAGATCAGAT 
GCTGGGCACG ATGTTGCTAT CCTTCACGGT GACGACAGTC AACTCTTCGA AAATTCAATG 
CAATTGGATC AGAAATCTCA ATTGCAAAAT GATGAGACTC AAATTCTTGA GAATAAAAAG 
AAGGCAGCTG TTGTCATTCA GAAAATGATA CGTGGTTTCA TTGCACGGCG TAAATTCCAA 
ATGGAAATTA GCAATATTCG CAATCGCATG ATCCAATACA ATCACATATT GGCGCAAGAA 
GACGAGCAAA TTGGTATAGA AGAAATGGAG GATAAATCAG TCGAGGCAAA GCTAAAGAAA 
TGCGCTCTTC ATGGACTCAC AAATGATAAC CTTCACGTCG TTCATGTTGC TGCAACTGTC 
ATCGACCGAG TCACCGATCT TGTTCCATCA CTTTTGGAAA AGCTTGTCGT CGAACTGAAT 
GGCATCAAGT CGATCACTGA TGTTTTGATT GTGGCGGATC GAGGATTCTC TTATACGAGC 
ATTCTTCATC CATTATTGAA TGTTCTCGAA AAATTATTCA CGAAGATTCC CGAAGAAGTC 
ACCAATTCGC ATATTCTCCC TCACTTGAAG ATTTTGTCGC CTCGCTTGAT GCTACTGATG 
CTGAAGCACT CGGAAGTCAA GGAATTGTTT ATTCCTATCA TTAACACCTT GACTTCAATT 
GCTAGAAGAT TCCCAGACAA AAAGCAATCG AGTTTTTCTT CATCAAGCTA CTCAATCAAG 
CAGTCGCTCA AAAAAGTCAA GGATCAGGAG TGTCGGAATA AATTGGTCAG ATTGGAAAGC 
ATATGCGAAG ATATGCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_492467.1
CDS	8..3568
Translation	MDNNVEDKKEAMLKRLANRHEKRLLDQVKSNTKKIDLRATERAFLESSPTSMNMRTPLNPSISSSVSDSPILSYDEKANKQIIALATWCNTMMELDVSEEMDLGESKAEACRNIQKMLKKRSDTSEVENTQENARRRYQRIFEKNDPEVVKKKCKQLLDDSGMDASIKDLLSKNNVAIRKEHAVYNDIGLQTTLLHTFLSFHPAWLKTALEAIFNTRIDAQPKHLMKKLSQFFLDLVFSNPTMLKNKKFAQGSGKPIITEAGKEALHKHFLSVSMKLMFLIETAHTHRVIPNLTRIFTKSSHFNCLDDVFSELTKELLTGSSATFKKAFAKVGFIPTYRQSFIENYDYQAKGFSDFSDGLILAKLLETVGEMPHGQILLKLRDPAGDRIRKIGNVKIVLQEMSSLGVPTDNVNAESIVGGKKDEILSILWAIIGVRVAKEQRIKVTRVSEERTPKKRRSAVHDDMSSEVLKMCKIYGRQMEIEVMDLDSLSDGCLLAKLWTTFGTNSTPIQDYDGLSLWEKVVSVAELELCIQRGLDQNMALFVKMFLERLGMIQDLNEKATKIQRMWKAYVQRKNTPKLYFIVQQLLADSSIPRNRSVSPFSNNVTFTVPRTPRNNILTERPSLSQIPSSRQSMDSTFNDATFTVSRDSIESMNKMQKTPLRGTFTRKTIAMVIEEEDDSENNETVVPSTLKKRTVVRMEHNAEVFREQDEDDENQDKDTVAPSAENLDSPPSDIPLETLSSIPSASQSAIFLQDSETGKEMHVPKAEDVGVVVLEASDSPVALEGNNEASYDGQKIENLETFEIKEGKTQEDLPSKSPMDPTQTSGSPLVEFRMTEEQERLEMLFQSLSEDQKNFVKTNNLSVSIEDDANTPELRRILRQTRELKRKQQEIARKLGNIERNALAVRDGGEDSSDSRSDAGHDVAILHGDDSQLFENSMQLDQKSQLQNDETQILENKKKAAVVIQKMIRGFIARRKFQMEISNIRNRMIQYNHILAQEDEQIGIEEMEDKSVEAKLKKCALHGLTNDNLHVVHVAATVIDRVTDLVPSLLEKLVVELNGIKSITDVLIVADRGFSYTSILHPLLNVLEKLFTKIPEEVTNSHILPHLKILSPRLMLLMLKHSEVKELFIPIINTLTSIARRFPDKKQSSFSSSSYSIKQSLKKVKDQECRNKLVRLESICEDML

Target ORF information:

RefSeq Version	NM_060066.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Calponin-homology (CH) domain-containing protein (aspm-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_060066.4

ORF Insert Sequence:

ATGGATAATA ACGTTGAGGA TAAAAAAGAG GCAATGCTCA AACGATTAGC AAATCGACAC 
GAGAAACGCC TTCTTGATCA AGTCAAATCG AACACAAAGA AGATCGATTT GAGAGCAACC 
GAGCGAGCCT TTTTGGAGTC GAGTCCGACT TCAATGAATA TGCGAACTCC TCTCAATCCT 
TCAATCAGCT CTTCTGTATC AGATTCCCCG ATTCTATCAT ATGATGAGAA AGCCAACAAG 
CAAATTATTG CACTCGCCAC GTGGTGCAAC ACAATGATGG AATTGGATGT GTCTGAAGAG 
ATGGATCTAG GAGAGTCGAA AGCAGAAGCC TGCAGAAATA TCCAAAAAAT GTTGAAGAAA 
AGATCAGATA CGTCAGAAGT GGAAAATACA CAGGAAAATG CACGCAGGAG ATATCAACGG 
ATTTTTGAGA AAAATGATCC AGAAGTTGTG AAGAAAAAGT GCAAGCAGCT TCTAGACGAT 
TCAGGAATGG ATGCAAGCAT CAAGGATCTT CTGTCCAAAA ACAACGTCGC AATCCGAAAA 
GAACATGCCG TATATAATGA CATAGGCCTC CAAACAACTC TTCTTCACAC ATTTCTATCA 
TTCCATCCTG CTTGGCTGAA AACTGCTCTT GAAGCAATCT TTAACACGAG AATTGACGCT 
CAACCGAAGC ATTTAATGAA AAAACTTTCT CAGTTTTTCC TCGATCTAGT TTTCTCAAAT 
CCGACGATGC TAAAGAACAA GAAATTTGCA CAAGGTTCTG GAAAACCGAT CATCACTGAG 
GCTGGCAAAG AAGCTCTACA CAAGCATTTT CTGTCCGTCT CGATGAAGCT GATGTTCTTG 
ATTGAAACGG CACACACTCA TCGAGTTATC CCGAATCTGA CGAGAATCTT CACGAAGTCC 
TCTCATTTCA ATTGTCTCGA CGATGTGTTC TCCGAACTGA CGAAGGAATT GTTGACTGGA 
AGCTCTGCTA CTTTCAAAAA AGCGTTTGCA AAAGTCGGTT TTATTCCGAC TTACCGACAA 
TCATTCATTG AAAATTACGA CTACCAAGCG AAAGGATTTT CAGATTTCTC GGATGGTCTA 
ATTTTGGCAA AGCTGTTGGA AACTGTCGGA GAGATGCCGC ATGGTCAAAT TCTGTTGAAG 
TTACGTGATC CAGCAGGCGA CCGCATCCGC AAAATTGGAA ACGTGAAGAT TGTACTCCAG 
GAGATGTCTT CTTTGGGTGT TCCAACGGAT AATGTTAACG CCGAATCAAT CGTTGGCGGA 
AAGAAAGACG AGATTTTGTC TATTTTGTGG GCTATTATCG GAGTTCGTGT GGCCAAGGAA 
CAAAGAATCA AGGTGACGAG AGTTTCTGAA GAAAGAACTC CGAAAAAGCG GCGATCTGCT 
GTCCACGATG ACATGTCTTC TGAAGTATTA AAAATGTGCA AGATTTACGG AAGACAGATG 
GAAATCGAAG TCATGGACTT GGATTCACTC TCCGATGGGT GCCTTCTGGC CAAGTTGTGG 
ACCACTTTCG GAACAAATTC AACACCGATT CAGGACTATG ATGGACTCAG TCTGTGGGAG 
AAAGTTGTTT CAGTTGCCGA GTTGGAGCTT TGTATTCAGC GAGGACTTGA TCAGAACATG 
GCTCTTTTCG TAAAAATGTT TCTGGAACGA CTGGGTATGA TTCAAGATCT AAATGAGAAA 
GCGACCAAGA TTCAACGAAT GTGGAAGGCC TACGTGCAAA GAAAGAATAC TCCCAAGCTG 
TATTTCATTG TACAACAACT TCTTGCCGAC TCATCAATTC CTCGAAACCG AAGTGTTTCT 
CCATTCTCTA ATAATGTCAC TTTCACAGTA CCGAGGACTC CACGGAATAA TATTCTGACT 
GAAAGACCAT CTCTATCACA AATCCCATCG TCTCGCCAAT CAATGGATTC TACCTTCAAC 
GATGCGACGT TTACTGTTTC ACGTGATTCA ATTGAAAGCA TGAACAAGAT GCAGAAAACT 
CCACTGAGAG GTACATTCAC AAGAAAAACA ATTGCGATGG TTATCGAGGA AGAGGATGAT 
TCGGAAAATA ACGAAACGGT CGTACCAAGT ACATTGAAAA AGAGAACCGT TGTCAGAATG 
GAGCACAATG CGGAAGTATT CAGAGAACAA GATGAAGATG ATGAAAACCA AGACAAAGAC 
ACTGTTGCTC CTTCCGCTGA GAACTTGGAT AGTCCACCTT CAGACATCCC ATTAGAAACT 
CTTTCTTCTA TTCCTTCCGC TTCCCAATCT GCCATATTTC TTCAAGACTC TGAAACTGGC 
AAAGAGATGC ATGTCCCTAA AGCTGAAGAT GTCGGCGTCG TAGTTCTTGA AGCATCAGAT 
TCTCCGGTTG CACTAGAAGG GAACAATGAA GCAAGCTATG ATGGTCAGAA AATTGAGAAC 
TTAGAAACTT TTGAAATCAA AGAGGGCAAG ACGCAAGAAG ATCTACCTTC AAAATCTCCT 
ATGGATCCTA CGCAGACAAG CGGAAGCCCA TTGGTTGAAT TCAGAATGAC AGAAGAACAG 
GAGCGTTTAG AGATGCTTTT CCAGTCATTA TCAGAAGATC AGAAGAACTT CGTCAAGACG 
AATAACCTTA GTGTGTCTAT TGAGGACGAT GCCAACACTC CTGAACTTAG AAGGATCTTG 
AGACAAACCA GAGAACTCAA GAGAAAGCAA CAGGAAATTG CGAGAAAACT GGGAAACATC 
GAGAGAAATG CGTTAGCTGT TCGTGATGGC GGGGAGGATT CGTCCGACAG TAGATCAGAT 
GCTGGGCACG ATGTTGCTAT CCTTCACGGT GACGACAGTC AACTCTTCGA AAATTCAATG 
CAATTGGATC AGAAATCTCA ATTGCAAAAT GATGAGACTC AAATTCTTGA GAATAAAAAG 
AAGGCAGCTG TTGTCATTCA GAAAATGATA CGTGGTTTCA TTGCACGGCG TAAATTCCAA 
ATGGAAATTA GCAATATTCG CAATCGCATG ATCCAATACA ATCACATATT GGCGCAAGAA 
GACGAGCAAA TTGGTATAGA AGAAATGGAG GATAAATCAG TCGAGGCAAA GCTAAAGAAA 
TGCGCTCTTC ATGGACTCAC AAATGATAAC CTTCACGTCG TTCATGTTGC TGCAACTGTC 
ATCGACCGAG TCACCGATCT TGTTCCATCA CTTTTGGAAA AGCTTGTCGT CGAACTGAAT 
GGCATCAAGT CGATCACTGA TGTTTTGATT GTGGCGGATC GAGGATTCTC TTATACGAGC 
ATTCTTCATC CATTATTGAA TGTTCTCGAA AAATTATTCA CGAAGATTCC CGAAGAAGTC 
ACCAATTCGC ATATTCTCCC TCACTTGAAG ATTTTGTCGC CTCGCTTGAT GCTACTGATG 
CTGAAGCACT CGGAAGTCAA GGAATTGTTT ATTCCTATCA TTAACACCTT GACTTCAATT 
GCTAGAAGAT TCCCAGACAA AAAGCAATCG AGTTTTTCTT CATCAAGCTA CTCAATCAAG 
CAGTCGCTCA AAAAAGTCAA GGATCAGGAG TGTCGGAATA AATTGGTCAG ATTGGAAAGC 
ATATGCGAAG ATATGCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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aspm-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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