let-502 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	let-502
Entrez Gene ID	172088
Full Name	Rho-associated protein kinase let-502
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_059039.6	NP_491440.1	Rho-associated protein kinase let-502
NM_059039.5	NP_491440.1	Rho-associated protein kinase let-502

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rock2	NP_033098.2
Drosophila melanogaster (fruit fly)	rok	NP_536796.2
Caenorhabditis elegans (roundworm)	let-502	NP_491440.1
Pan troglodytes (chimpanzee)	ROCK2	XP_525689.3
Rattus norvegicus (Norway rat)	Rock2	NP_037154.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000406	XP_310694.5
Xenopus tropicalis (tropical clawed frog)	rock2	XP_004914525.1
Homo sapiens (human)	ROCK2	NP_004841.2
	ROCK2	XP_001096931.2
Canis lupus familiaris (dog)	ROCK2	XP_540083.4
Danio rerio (zebrafish)	rock2a	NP_777288.1
Bos taurus (cattle)	ROCK2	NP_776877.1
Gallus gallus (chicken)	ROCK2	XP_004935820.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MLC-4-containing myosin II is a Rho GTPase-regulated generator of contractility (of the myoepithelial sheath in the Caenorhabditis elegans somatic gonad) and that the two myosin II populations coordinate to produce actomyosin contractility for successful ovulation.
Title: Two distinct myosin II populations coordinate ovulatory contraction of the myoepithelial sheath in the Caenorhabditis elegans somatic gonad.

RHGF-2 acts through the Rho-binding kinase LET-502 to mediate embryonic elongation in C. elegans
Title: The Rho guanine exchange factor RHGF-2 acts through the Rho-binding kinase LET-502 to mediate embryonic elongation in C. elegans.

pix-1 has a role in controlling early elongation in parallel with mel-11 and let-502 in Caenorhabditis elegans
Title: pix-1 controls early elongation in parallel with mel-11 and let-502 in Caenorhabditis elegans.

Data show that combined loss of ROCK and PAK, or ROCK and MRCK, completely prevented embryonic elongation, but a constitutively active form of MLC-4 could only rescue a lack of MRCK.
Title: Myosin II regulation during C. elegans embryonic elongation: LET-502/ROCK, MRCK-1 and PAK-1, three kinases with different roles.

actomyosin-generated tension must be moderated in two out of the three sets of epidermal cells surrounding the C. elegans embryo to achieve morphogenesis
Title: The RhoGAP RGA-2 and LET-502/ROCK achieve a balance of actomyosin-dependent forces in C. elegans epidermis to control morphogenesis.

The following let-502 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the let-502 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa336505	NM_059039.6 Latest version!	Caenorhabditis elegans Rho-associated protein kinase let-502 (let-502), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa336505	NM_059039.5	Caenorhabditis elegans Rho-associated protein kinase let-502 (let-502), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

let-502 ( NM_059039.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491440.1 Caenorhabditis elegans Rho-associated protein kinase let-502 (let-502), mRNA.

Price & Availability↑

CloneID	OCa336505
Clone ID Related Accession (Same CDS sequence)	NM_059039.6 , NM_059039.5
Accession Version	NM_059039.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Rho-associated protein kinase let-502
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059039.5.

ATGGAGCAGG ATGAGCTGCG TGATCAGCTG GTGGATCCAA AATCCCCGAT AAACATCGAA 
AGCTTGCTCG ACACCATCAC AGCGCTCGTC AATGATTGCA AAATTCCGGT GCTCATGCGG 
ATGAAAAGCG TCGACAACTT CATTAGCAGA TATGAACGAG TTGTCGAAAG TTTGGCCGCT 
CTTCGAATGA AAGCTGCCGA TTTCAGACAA TTGAAGGTTA TTGGAAGAGG AGCATTCGGA 
GAAGTTCATC TGGTTCGACA CACACGAACG AACACTGTGT ACGCGATGAA GATGCTCAAT 
AAGGATGACA TGATCAAACG AGCTGACTCT GCATTCTTCT GGGAGGAACG TGATATTATG 
GCTCACGCGA ATAGCGAATG GATCGTGAGG CTTCAGTATG CATTCCAGGA TCCTCGTCAT 
TTGTACATGG TGATGGAATA TATGCCAGGT GGAGATCTCG TGAATCTGAT GACATCCTAT 
GAAGTATCGG AAAAGTGGAC TCGCTTTTAT ACGGCTGAAA TTGTCGAGGC GCTGGCCGCC 
CTTCACAGTA TGGGATATAT TCATCGAGAT GTGAAGCCGG ACAATATGCT CATTTCGATC 
AGCGGACACA TAAAATTGGC AGATTTTGGA ACATGCGTGA AGATGAATGC CAATGGAGTT 
GTGCGATGCT CCACAGCAGT CGGAACTCCT GATTACATAT CACCGGAAGT TTTGAGAAAT 
CAAGGACAAG ATGCTGAATT TGGAAAAGAA GTCGATTGGT GGTCTGTTGG TGTGTTCATA 
TATGAAATGC TTGTTGGAGA AACACCATTC TATGCCGAGG CACTTGTATC AACTTATACA 
AATATTATGA ATCATAAAAC AAGTCTGAAA TTTCCTGATG AGCCATTAAT TTCTACCCAA 
GCCAAGGATA TTATCAAGAA ATTCCTCTCG GCGGCTCCAG ATCGTCTTGG AAGAAATAGC 
GTCGATGATA TTCGAAATCA CAAGTTTTTT GTTAATGACG AATGGACATT TGCCACTCTC 
AGAGAAGCAT CTCCACCGGT CATTCCTTCA TTAAAATCGG ACGATGACAC GACACATTTC 
GAAGAAATCG AGACCCGTGA TCGAGATAAT GCGGGAGACT TTCAATTGCC GAAGACATTT 
AACGGAAATC AACTACCATT CATCGGCTTT ACTTATTCGA ACGAGTACAG TCCTGTGAAG 
AATCTTCTGA AGGGCCATGG TGCTGGATCA AAACAGAACG GAATTGAACA GCATAAACCG 
CAAACTGTTG TTGAGCAACC ATTGACGAAT GGGCATGCGT CTGGTGTTCC TGAAGAAAAA 
TATGAAGCAG TTAAGATGGA GTTGGACTCG AAAAACCGAG AATTCGAACT TTTGAAGGAT 
AGCATTGCTC GAAATGAGAT CCGAGCGAAG ATGATCGAAA ATGAGAAGAA CTCATTGTCC 
ACAAAAATCT CCGATCTCGA ACGAGAACTC AAGGATAATA AAGATAAACT GAGGCATGGC 
GCTGATTCGG ATGCAAAAGT TAACGAATTG GCTGTCGAGT TACGAATGTC CAAAGAGTAC 
AATAGTGAGA TGGAGAGCGA ATTGAGCAAG TTTCGGGACA AGTGTGAGCA GCTCAAGGAA 
GATCTTCGCA AAAAATCTGG AGAGCTTGCT CAGGAGAAGA ATGAAACTCA ACGGGTATTC 
CAGCAGAAGA AGGACGCTGA TGAAGCATTT GCCGAGATTA AACGGGATTA TGAACTATTA 
CAAACCAGAG AAAATGAAAA GTCTGTTCAA TTGAAGAAAG CGCTTGACGA ACGAAAAGAG 
AACGGAGCCT ATCAGCAAAG TGTTGCAAAG GCGACAGATG CTGAATGGGA GCGAAAAATG 
CAGTTTTATG AGAAGCAGTT GGAGCATGCG AATGATGAAC GGAAAAGAGA AGAGCAAAAG 
CGAACGGCTG CAGAATTCGA TCAAAGCAGG GTGGCAAGGA AATTGGCTGG GATTGAAGCG 
AATTATGAGC TGCTGCAGAA TGACTATAAA AGTATGAAGG AAGCAAGGAA GGATCTTGAA 
AGAGATCTTC AGGATGTCAT TACTGAGAAA AGGCGACTTG AAATACGTGT CGAACAGTTG 
ATGGACAGCC GGAACACCGA CGAACGGGTA TTATCGCTCT GTCAGGATGA GCTCGTGGAA 
AGTCAAGAGG AAGCAAAGTA TAAGGAAGAT GGATTGAGAG GAAAGATTGA CGGATTTAAA 
CATGAACTTG AGAATGAGAA GATGAAGACT CAAACTTTGG AGGAAAATCT TCTGGTAGCT 
GATAAGGAGA GGGGCATGCT GAAAATGGAA GTTCAAGAGC TGATGCAAAG GCATAAATGG 
GAGATTACCA ACAAAGATCA AACGCTGAAA CATCTTGAGA CTCAACTAGA CGAAATAAAG 
CAACAATCAA AGATCGAGTC GTCTGAGCAG GAATCGAATG ATAAGCAGAC GATCGCTGAT 
CTTCGAAAGA AGTTGGATCT AGAGAAAGCT CACAAGAAGG CTGTAATCAA TAAGTTAGAA 
GAGGAAATGG CAAAGCGGCA ACCATTGAAA AAGGGTGAAA AGGGTGTCAC AAAGTCTGCG 
TTGATAAAGA AAGAACGAGA GATTATGGCT CTCGAACAAG AACGCGATAC GATGAGTAAA 
AGGATTGCCG CTCTGTTTTA CGAAAATGAT AAGCAAGCCG AGCATTTCAA CATTGCGATT 
CAAGATATGC AGACTACACA AGACGCTTTA CGAGATGAGC TTAAAGAATG CAAAGAGGAG 
TTAGCCAACA GAAATGTGAA TACTCGATAT GAAGATAAAC GATCATTGGA TTCTCGAGAA 
GGGATTCCAT CTTCGTTAAG CAATCAACAT ATTCAAATGG AAGGCTGGCT ATCGCTCAGA 
GATAATACAA AGAAGTCACG GAAACCGAAG TGGACCAACT GCTTTGTTGC GCTTAACGAG 
TATTCATTCA CAATCTATGT CGACGAAAAA GCCGTGTCTG TGATTCTTTT GATTGAAGCC 
GGAGCTATGG CTCATGTTCG ACACGTCACT GCTGCTGATT TGAGAAATGT CGACGATAGT 
CAGTTACCTA AGATATTCCA TATCATGTAT GACGACGCGT CGTGCAATTC GTCACGACAC 
GCCTCCAATT CAGATCTCTC AATGATTGAA TCGTTCCGAG AAGAAAGTTG GAAGCGGCAC 
GATTTCCAAG AGCTCTCCTA TCACATACGC ACGTTTTGTG ATGTCTGCAA TAAGAAATTG 
TCTGACATTA TCCGACCGAC GCCTGCATTC GAATGCAAAA ATTGCCATTT CAAGACACAC 
AAGGATCATG TTGCTCAAGG ATCGTTGCCA ATGTGTCGAT ACAACACGGG TCTCTCTCGT 
GAACTCGTTC TTATGGCTCC ACAAACGGAT AACTGCAATA AATGGGTATC ACAACTTCGA 
CGCTTCATTG AATCATCACG ATCAGCGGCA GTTTCAGTGT CTCGTGTCTC TTCACGACGA 
CATGCTCCAG GATCGTCAAA CTCTTCCACA ATCTATCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491440.1
CDS	61..3582
Translation	MEQDELRDQLVDPKSPINIESLLDTITALVNDCKIPVLMRMKSVDNFISRYERVVESLAALRMKAADFRQLKVIGRGAFGEVHLVRHTRTNTVYAMKMLNKDDMIKRADSAFFWEERDIMAHANSEWIVRLQYAFQDPRHLYMVMEYMPGGDLVNLMTSYEVSEKWTRFYTAEIVEALAALHSMGYIHRDVKPDNMLISISGHIKLADFGTCVKMNANGVVRCSTAVGTPDYISPEVLRNQGQDAEFGKEVDWWSVGVFIYEMLVGETPFYAEALVSTYTNIMNHKTSLKFPDEPLISTQAKDIIKKFLSAAPDRLGRNSVDDIRNHKFFVNDEWTFATLREASPPVIPSLKSDDDTTHFEEIETRDRDNAGDFQLPKTFNGNQLPFIGFTYSNEYSPVKNLLKGHGAGSKQNGIEQHKPQTVVEQPLTNGHASGVPEEKYEAVKMELDSKNREFELLKDSIARNEIRAKMIENEKNSLSTKISDLERELKDNKDKLRHGADSDAKVNELAVELRMSKEYNSEMESELSKFRDKCEQLKEDLRKKSGELAQEKNETQRVFQQKKDADEAFAEIKRDYELLQTRENEKSVQLKKALDERKENGAYQQSVAKATDAEWERKMQFYEKQLEHANDERKREEQKRTAAEFDQSRVARKLAGIEANYELLQNDYKSMKEARKDLERDLQDVITEKRRLEIRVEQLMDSRNTDERVLSLCQDELVESQEEAKYKEDGLRGKIDGFKHELENEKMKTQTLEENLLVADKERGMLKMEVQELMQRHKWEITNKDQTLKHLETQLDEIKQQSKIESSEQESNDKQTIADLRKKLDLEKAHKKAVINKLEEEMAKRQPLKKGEKGVTKSALIKKEREIMALEQERDTMSKRIAALFYENDKQAEHFNIAIQDMQTTQDALRDELKECKEELANRNVNTRYEDKRSLDSREGIPSSLSNQHIQMEGWLSLRDNTKKSRKPKWTNCFVALNEYSFTIYVDEKAVSVILLIEAGAMAHVRHVTAADLRNVDDSQLPKIFHIMYDDASCNSSRHASNSDLSMIESFREESWKRHDFQELSYHIRTFCDVCNKKLSDIIRPTPAFECKNCHFKTHKDHVAQGSLPMCRYNTGLSRELVLMAPQTDNCNKWVSQLRRFIESSRSAAVSVSRVSSRRHAPGSSNSSTIYQ

Target ORF information:

RefSeq Version	NM_059039.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Rho-associated protein kinase let-502 (let-502), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059039.6

ORF Insert Sequence:

ATGGAGCAGG ATGAGCTGCG TGATCAGCTG GTGGATCCAA AATCCCCGAT AAACATCGAA 
AGCTTGCTCG ACACCATCAC AGCGCTCGTC AATGATTGCA AAATTCCGGT GCTCATGCGG 
ATGAAAAGCG TCGACAACTT CATTAGCAGA TATGAACGAG TTGTCGAAAG TTTGGCCGCT 
CTTCGAATGA AAGCTGCCGA TTTCAGACAA TTGAAGGTTA TTGGAAGAGG AGCATTCGGA 
GAAGTTCATC TGGTTCGACA CACACGAACG AACACTGTGT ACGCGATGAA GATGCTCAAT 
AAGGATGACA TGATCAAACG AGCTGACTCT GCATTCTTCT GGGAGGAACG TGATATTATG 
GCTCACGCGA ATAGCGAATG GATCGTGAGG CTTCAGTATG CATTCCAGGA TCCTCGTCAT 
TTGTACATGG TGATGGAATA TATGCCAGGT GGAGATCTCG TGAATCTGAT GACATCCTAT 
GAAGTATCGG AAAAGTGGAC TCGCTTTTAT ACGGCTGAAA TTGTCGAGGC GCTGGCCGCC 
CTTCACAGTA TGGGATATAT TCATCGAGAT GTGAAGCCGG ACAATATGCT CATTTCGATC 
AGCGGACACA TAAAATTGGC AGATTTTGGA ACATGCGTGA AGATGAATGC CAATGGAGTT 
GTGCGATGCT CCACAGCAGT CGGAACTCCT GATTACATAT CACCGGAAGT TTTGAGAAAT 
CAAGGACAAG ATGCTGAATT TGGAAAAGAA GTCGATTGGT GGTCTGTTGG TGTGTTCATA 
TATGAAATGC TTGTTGGAGA AACACCATTC TATGCCGAGG CACTTGTATC AACTTATACA 
AATATTATGA ATCATAAAAC AAGTCTGAAA TTTCCTGATG AGCCATTAAT TTCTACCCAA 
GCCAAGGATA TTATCAAGAA ATTCCTCTCG GCGGCTCCAG ATCGTCTTGG AAGAAATAGC 
GTCGATGATA TTCGAAATCA CAAGTTTTTT GTTAATGACG AATGGACATT TGCCACTCTC 
AGAGAAGCAT CTCCACCGGT CATTCCTTCA TTAAAATCGG ACGATGACAC GACACATTTC 
GAAGAAATCG AGACCCGTGA TCGAGATAAT GCGGGAGACT TTCAATTGCC GAAGACATTT 
AACGGAAATC AACTACCATT CATCGGCTTT ACTTATTCGA ACGAGTACAG TCCTGTGAAG 
AATCTTCTGA AGGGCCATGG TGCTGGATCA AAACAGAACG GAATTGAACA GCATAAACCG 
CAAACTGTTG TTGAGCAACC ATTGACGAAT GGGCATGCGT CTGGTGTTCC TGAAGAAAAA 
TATGAAGCAG TTAAGATGGA GTTGGACTCG AAAAACCGAG AATTCGAACT TTTGAAGGAT 
AGCATTGCTC GAAATGAGAT CCGAGCGAAG ATGATCGAAA ATGAGAAGAA CTCATTGTCC 
ACAAAAATCT CCGATCTCGA ACGAGAACTC AAGGATAATA AAGATAAACT GAGGCATGGC 
GCTGATTCGG ATGCAAAAGT TAACGAATTG GCTGTCGAGT TACGAATGTC CAAAGAGTAC 
AATAGTGAGA TGGAGAGCGA ATTGAGCAAG TTTCGGGACA AGTGTGAGCA GCTCAAGGAA 
GATCTTCGCA AAAAATCTGG AGAGCTTGCT CAGGAGAAGA ATGAAACTCA ACGGGTATTC 
CAGCAGAAGA AGGACGCTGA TGAAGCATTT GCCGAGATTA AACGGGATTA TGAACTATTA 
CAAACCAGAG AAAATGAAAA GTCTGTTCAA TTGAAGAAAG CGCTTGACGA ACGAAAAGAG 
AACGGAGCCT ATCAGCAAAG TGTTGCAAAG GCGACAGATG CTGAATGGGA GCGAAAAATG 
CAGTTTTATG AGAAGCAGTT GGAGCATGCG AATGATGAAC GGAAAAGAGA AGAGCAAAAG 
CGAACGGCTG CAGAATTCGA TCAAAGCAGG GTGGCAAGGA AATTGGCTGG GATTGAAGCG 
AATTATGAGC TGCTGCAGAA TGACTATAAA AGTATGAAGG AAGCAAGGAA GGATCTTGAA 
AGAGATCTTC AGGATGTCAT TACTGAGAAA AGGCGACTTG AAATACGTGT CGAACAGTTG 
ATGGACAGCC GGAACACCGA CGAACGGGTA TTATCGCTCT GTCAGGATGA GCTCGTGGAA 
AGTCAAGAGG AAGCAAAGTA TAAGGAAGAT GGATTGAGAG GAAAGATTGA CGGATTTAAA 
CATGAACTTG AGAATGAGAA GATGAAGACT CAAACTTTGG AGGAAAATCT TCTGGTAGCT 
GATAAGGAGA GGGGCATGCT GAAAATGGAA GTTCAAGAGC TGATGCAAAG GCATAAATGG 
GAGATTACCA ACAAAGATCA AACGCTGAAA CATCTTGAGA CTCAACTAGA CGAAATAAAG 
CAACAATCAA AGATCGAGTC GTCTGAGCAG GAATCGAATG ATAAGCAGAC GATCGCTGAT 
CTTCGAAAGA AGTTGGATCT AGAGAAAGCT CACAAGAAGG CTGTAATCAA TAAGTTAGAA 
GAGGAAATGG CAAAGCGGCA ACCATTGAAA AAGGGTGAAA AGGGTGTCAC AAAGTCTGCG 
TTGATAAAGA AAGAACGAGA GATTATGGCT CTCGAACAAG AACGCGATAC GATGAGTAAA 
AGGATTGCCG CTCTGTTTTA CGAAAATGAT AAGCAAGCCG AGCATTTCAA CATTGCGATT 
CAAGATATGC AGACTACACA AGACGCTTTA CGAGATGAGC TTAAAGAATG CAAAGAGGAG 
TTAGCCAACA GAAATGTGAA TACTCGATAT GAAGATAAAC GATCATTGGA TTCTCGAGAA 
GGGATTCCAT CTTCGTTAAG CAATCAACAT ATTCAAATGG AAGGCTGGCT ATCGCTCAGA 
GATAATACAA AGAAGTCACG GAAACCGAAG TGGACCAACT GCTTTGTTGC GCTTAACGAG 
TATTCATTCA CAATCTATGT CGACGAAAAA GCCGTGTCTG TGATTCTTTT GATTGAAGCC 
GGAGCTATGG CTCATGTTCG ACACGTCACT GCTGCTGATT TGAGAAATGT CGACGATAGT 
CAGTTACCTA AGATATTCCA TATCATGTAT GACGACGCGT CGTGCAATTC GTCACGACAC 
GCCTCCAATT CAGATCTCTC AATGATTGAA TCGTTCCGAG AAGAAAGTTG GAAGCGGCAC 
GATTTCCAAG AGCTCTCCTA TCACATACGC ACGTTTTGTG ATGTCTGCAA TAAGAAATTG 
TCTGACATTA TCCGACCGAC GCCTGCATTC GAATGCAAAA ATTGCCATTT CAAGACACAC 
AAGGATCATG TTGCTCAAGG ATCGTTGCCA ATGTGTCGAT ACAACACGGG TCTCTCTCGT 
GAACTCGTTC TTATGGCTCC ACAAACGGAT AACTGCAATA AATGGGTATC ACAACTTCGA 
CGCTTCATTG AATCATCACG ATCAGCGGCA GTTTCAGTGT CTCGTGTCTC TTCACGACGA 
CATGCTCCAG GATCGTCAAA CTCTTCCACA ATCTATCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

let-502 ( NM_059039.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491440.1 Caenorhabditis elegans Rho-associated protein kinase let-502 (let-502), partial mRNA.

Price & Availability↑

CloneID	OCa336505
Clone ID Related Accession (Same CDS sequence)	NM_059039.6 , NM_059039.5
Accession Version	NM_059039.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Rho-associated protein kinase let-502
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_059039.4. COMPLETENESS: incomplete on both ends.

ATGGAGCAGG ATGAGCTGCG TGATCAGCTG GTGGATCCAA AATCCCCGAT AAACATCGAA 
AGCTTGCTCG ACACCATCAC AGCGCTCGTC AATGATTGCA AAATTCCGGT GCTCATGCGG 
ATGAAAAGCG TCGACAACTT CATTAGCAGA TATGAACGAG TTGTCGAAAG TTTGGCCGCT 
CTTCGAATGA AAGCTGCCGA TTTCAGACAA TTGAAGGTTA TTGGAAGAGG AGCATTCGGA 
GAAGTTCATC TGGTTCGACA CACACGAACG AACACTGTGT ACGCGATGAA GATGCTCAAT 
AAGGATGACA TGATCAAACG AGCTGACTCT GCATTCTTCT GGGAGGAACG TGATATTATG 
GCTCACGCGA ATAGCGAATG GATCGTGAGG CTTCAGTATG CATTCCAGGA TCCTCGTCAT 
TTGTACATGG TGATGGAATA TATGCCAGGT GGAGATCTCG TGAATCTGAT GACATCCTAT 
GAAGTATCGG AAAAGTGGAC TCGCTTTTAT ACGGCTGAAA TTGTCGAGGC GCTGGCCGCC 
CTTCACAGTA TGGGATATAT TCATCGAGAT GTGAAGCCGG ACAATATGCT CATTTCGATC 
AGCGGACACA TAAAATTGGC AGATTTTGGA ACATGCGTGA AGATGAATGC CAATGGAGTT 
GTGCGATGCT CCACAGCAGT CGGAACTCCT GATTACATAT CACCGGAAGT TTTGAGAAAT 
CAAGGACAAG ATGCTGAATT TGGAAAAGAA GTCGATTGGT GGTCTGTTGG TGTGTTCATA 
TATGAAATGC TTGTTGGAGA AACACCATTC TATGCCGAGG CACTTGTATC AACTTATACA 
AATATTATGA ATCATAAAAC AAGTCTGAAA TTTCCTGATG AGCCATTAAT TTCTACCCAA 
GCCAAGGATA TTATCAAGAA ATTCCTCTCG GCGGCTCCAG ATCGTCTTGG AAGAAATAGC 
GTCGATGATA TTCGAAATCA CAAGTTTTTT GTTAATGACG AATGGACATT TGCCACTCTC 
AGAGAAGCAT CTCCACCGGT CATTCCTTCA TTAAAATCGG ACGATGACAC GACACATTTC 
GAAGAAATCG AGACCCGTGA TCGAGATAAT GCGGGAGACT TTCAATTGCC GAAGACATTT 
AACGGAAATC AACTACCATT CATCGGCTTT ACTTATTCGA ACGAGTACAG TCCTGTGAAG 
AATCTTCTGA AGGGCCATGG TGCTGGATCA AAACAGAACG GAATTGAACA GCATAAACCG 
CAAACTGTTG TTGAGCAACC ATTGACGAAT GGGCATGCGT CTGGTGTTCC TGAAGAAAAA 
TATGAAGCAG TTAAGATGGA GTTGGACTCG AAAAACCGAG AATTCGAACT TTTGAAGGAT 
AGCATTGCTC GAAATGAGAT CCGAGCGAAG ATGATCGAAA ATGAGAAGAA CTCATTGTCC 
ACAAAAATCT CCGATCTCGA ACGAGAACTC AAGGATAATA AAGATAAACT GAGGCATGGC 
GCTGATTCGG ATGCAAAAGT TAACGAATTG GCTGTCGAGT TACGAATGTC CAAAGAGTAC 
AATAGTGAGA TGGAGAGCGA ATTGAGCAAG TTTCGGGACA AGTGTGAGCA GCTCAAGGAA 
GATCTTCGCA AAAAATCTGG AGAGCTTGCT CAGGAGAAGA ATGAAACTCA ACGGGTATTC 
CAGCAGAAGA AGGACGCTGA TGAAGCATTT GCCGAGATTA AACGGGATTA TGAACTATTA 
CAAACCAGAG AAAATGAAAA GTCTGTTCAA TTGAAGAAAG CGCTTGACGA ACGAAAAGAG 
AACGGAGCCT ATCAGCAAAG TGTTGCAAAG GCGACAGATG CTGAATGGGA GCGAAAAATG 
CAGTTTTATG AGAAGCAGTT GGAGCATGCG AATGATGAAC GGAAAAGAGA AGAGCAAAAG 
CGAACGGCTG CAGAATTCGA TCAAAGCAGG GTGGCAAGGA AATTGGCTGG GATTGAAGCG 
AATTATGAGC TGCTGCAGAA TGACTATAAA AGTATGAAGG AAGCAAGGAA GGATCTTGAA 
AGAGATCTTC AGGATGTCAT TACTGAGAAA AGGCGACTTG AAATACGTGT CGAACAGTTG 
ATGGACAGCC GGAACACCGA CGAACGGGTA TTATCGCTCT GTCAGGATGA GCTCGTGGAA 
AGTCAAGAGG AAGCAAAGTA TAAGGAAGAT GGATTGAGAG GAAAGATTGA CGGATTTAAA 
CATGAACTTG AGAATGAGAA GATGAAGACT CAAACTTTGG AGGAAAATCT TCTGGTAGCT 
GATAAGGAGA GGGGCATGCT GAAAATGGAA GTTCAAGAGC TGATGCAAAG GCATAAATGG 
GAGATTACCA ACAAAGATCA AACGCTGAAA CATCTTGAGA CTCAACTAGA CGAAATAAAG 
CAACAATCAA AGATCGAGTC GTCTGAGCAG GAATCGAATG ATAAGCAGAC GATCGCTGAT 
CTTCGAAAGA AGTTGGATCT AGAGAAAGCT CACAAGAAGG CTGTAATCAA TAAGTTAGAA 
GAGGAAATGG CAAAGCGGCA ACCATTGAAA AAGGGTGAAA AGGGTGTCAC AAAGTCTGCG 
TTGATAAAGA AAGAACGAGA GATTATGGCT CTCGAACAAG AACGCGATAC GATGAGTAAA 
AGGATTGCCG CTCTGTTTTA CGAAAATGAT AAGCAAGCCG AGCATTTCAA CATTGCGATT 
CAAGATATGC AGACTACACA AGACGCTTTA CGAGATGAGC TTAAAGAATG CAAAGAGGAG 
TTAGCCAACA GAAATGTGAA TACTCGATAT GAAGATAAAC GATCATTGGA TTCTCGAGAA 
GGGATTCCAT CTTCGTTAAG CAATCAACAT ATTCAAATGG AAGGCTGGCT ATCGCTCAGA 
GATAATACAA AGAAGTCACG GAAACCGAAG TGGACCAACT GCTTTGTTGC GCTTAACGAG 
TATTCATTCA CAATCTATGT CGACGAAAAA GCCGTGTCTG TGATTCTTTT GATTGAAGCC 
GGAGCTATGG CTCATGTTCG ACACGTCACT GCTGCTGATT TGAGAAATGT CGACGATAGT 
CAGTTACCTA AGATATTCCA TATCATGTAT GACGACGCGT CGTGCAATTC GTCACGACAC 
GCCTCCAATT CAGATCTCTC AATGATTGAA TCGTTCCGAG AAGAAAGTTG GAAGCGGCAC 
GATTTCCAAG AGCTCTCCTA TCACATACGC ACGTTTTGTG ATGTCTGCAA TAAGAAATTG 
TCTGACATTA TCCGACCGAC GCCTGCATTC GAATGCAAAA ATTGCCATTT CAAGACACAC 
AAGGATCATG TTGCTCAAGG ATCGTTGCCA ATGTGTCGAT ACAACACGGG TCTCTCTCGT 
GAACTCGTTC TTATGGCTCC ACAAACGGAT AACTGCAATA AATGGGTATC ACAACTTCGA 
CGCTTCATTG AATCATCACG ATCAGCGGCA GTTTCAGTGT CTCGTGTCTC TTCACGACGA 
CATGCTCCAG GATCGTCAAA CTCTTCCACA ATCTATCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491440.1
CDS	1..3522
Translation	MEQDELRDQLVDPKSPINIESLLDTITALVNDCKIPVLMRMKSVDNFISRYERVVESLAALRMKAADFRQLKVIGRGAFGEVHLVRHTRTNTVYAMKMLNKDDMIKRADSAFFWEERDIMAHANSEWIVRLQYAFQDPRHLYMVMEYMPGGDLVNLMTSYEVSEKWTRFYTAEIVEALAALHSMGYIHRDVKPDNMLISISGHIKLADFGTCVKMNANGVVRCSTAVGTPDYISPEVLRNQGQDAEFGKEVDWWSVGVFIYEMLVGETPFYAEALVSTYTNIMNHKTSLKFPDEPLISTQAKDIIKKFLSAAPDRLGRNSVDDIRNHKFFVNDEWTFATLREASPPVIPSLKSDDDTTHFEEIETRDRDNAGDFQLPKTFNGNQLPFIGFTYSNEYSPVKNLLKGHGAGSKQNGIEQHKPQTVVEQPLTNGHASGVPEEKYEAVKMELDSKNREFELLKDSIARNEIRAKMIENEKNSLSTKISDLERELKDNKDKLRHGADSDAKVNELAVELRMSKEYNSEMESELSKFRDKCEQLKEDLRKKSGELAQEKNETQRVFQQKKDADEAFAEIKRDYELLQTRENEKSVQLKKALDERKENGAYQQSVAKATDAEWERKMQFYEKQLEHANDERKREEQKRTAAEFDQSRVARKLAGIEANYELLQNDYKSMKEARKDLERDLQDVITEKRRLEIRVEQLMDSRNTDERVLSLCQDELVESQEEAKYKEDGLRGKIDGFKHELENEKMKTQTLEENLLVADKERGMLKMEVQELMQRHKWEITNKDQTLKHLETQLDEIKQQSKIESSEQESNDKQTIADLRKKLDLEKAHKKAVINKLEEEMAKRQPLKKGEKGVTKSALIKKEREIMALEQERDTMSKRIAALFYENDKQAEHFNIAIQDMQTTQDALRDELKECKEELANRNVNTRYEDKRSLDSREGIPSSLSNQHIQMEGWLSLRDNTKKSRKPKWTNCFVALNEYSFTIYVDEKAVSVILLIEAGAMAHVRHVTAADLRNVDDSQLPKIFHIMYDDASCNSSRHASNSDLSMIESFREESWKRHDFQELSYHIRTFCDVCNKKLSDIIRPTPAFECKNCHFKTHKDHVAQGSLPMCRYNTGLSRELVLMAPQTDNCNKWVSQLRRFIESSRSAAVSVSRVSSRRHAPGSSNSSTIYQ

Target ORF information:

RefSeq Version	NM_059039.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Rho-associated protein kinase let-502 (let-502), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059039.5

ORF Insert Sequence:

ATGGAGCAGG ATGAGCTGCG TGATCAGCTG GTGGATCCAA AATCCCCGAT AAACATCGAA 
AGCTTGCTCG ACACCATCAC AGCGCTCGTC AATGATTGCA AAATTCCGGT GCTCATGCGG 
ATGAAAAGCG TCGACAACTT CATTAGCAGA TATGAACGAG TTGTCGAAAG TTTGGCCGCT 
CTTCGAATGA AAGCTGCCGA TTTCAGACAA TTGAAGGTTA TTGGAAGAGG AGCATTCGGA 
GAAGTTCATC TGGTTCGACA CACACGAACG AACACTGTGT ACGCGATGAA GATGCTCAAT 
AAGGATGACA TGATCAAACG AGCTGACTCT GCATTCTTCT GGGAGGAACG TGATATTATG 
GCTCACGCGA ATAGCGAATG GATCGTGAGG CTTCAGTATG CATTCCAGGA TCCTCGTCAT 
TTGTACATGG TGATGGAATA TATGCCAGGT GGAGATCTCG TGAATCTGAT GACATCCTAT 
GAAGTATCGG AAAAGTGGAC TCGCTTTTAT ACGGCTGAAA TTGTCGAGGC GCTGGCCGCC 
CTTCACAGTA TGGGATATAT TCATCGAGAT GTGAAGCCGG ACAATATGCT CATTTCGATC 
AGCGGACACA TAAAATTGGC AGATTTTGGA ACATGCGTGA AGATGAATGC CAATGGAGTT 
GTGCGATGCT CCACAGCAGT CGGAACTCCT GATTACATAT CACCGGAAGT TTTGAGAAAT 
CAAGGACAAG ATGCTGAATT TGGAAAAGAA GTCGATTGGT GGTCTGTTGG TGTGTTCATA 
TATGAAATGC TTGTTGGAGA AACACCATTC TATGCCGAGG CACTTGTATC AACTTATACA 
AATATTATGA ATCATAAAAC AAGTCTGAAA TTTCCTGATG AGCCATTAAT TTCTACCCAA 
GCCAAGGATA TTATCAAGAA ATTCCTCTCG GCGGCTCCAG ATCGTCTTGG AAGAAATAGC 
GTCGATGATA TTCGAAATCA CAAGTTTTTT GTTAATGACG AATGGACATT TGCCACTCTC 
AGAGAAGCAT CTCCACCGGT CATTCCTTCA TTAAAATCGG ACGATGACAC GACACATTTC 
GAAGAAATCG AGACCCGTGA TCGAGATAAT GCGGGAGACT TTCAATTGCC GAAGACATTT 
AACGGAAATC AACTACCATT CATCGGCTTT ACTTATTCGA ACGAGTACAG TCCTGTGAAG 
AATCTTCTGA AGGGCCATGG TGCTGGATCA AAACAGAACG GAATTGAACA GCATAAACCG 
CAAACTGTTG TTGAGCAACC ATTGACGAAT GGGCATGCGT CTGGTGTTCC TGAAGAAAAA 
TATGAAGCAG TTAAGATGGA GTTGGACTCG AAAAACCGAG AATTCGAACT TTTGAAGGAT 
AGCATTGCTC GAAATGAGAT CCGAGCGAAG ATGATCGAAA ATGAGAAGAA CTCATTGTCC 
ACAAAAATCT CCGATCTCGA ACGAGAACTC AAGGATAATA AAGATAAACT GAGGCATGGC 
GCTGATTCGG ATGCAAAAGT TAACGAATTG GCTGTCGAGT TACGAATGTC CAAAGAGTAC 
AATAGTGAGA TGGAGAGCGA ATTGAGCAAG TTTCGGGACA AGTGTGAGCA GCTCAAGGAA 
GATCTTCGCA AAAAATCTGG AGAGCTTGCT CAGGAGAAGA ATGAAACTCA ACGGGTATTC 
CAGCAGAAGA AGGACGCTGA TGAAGCATTT GCCGAGATTA AACGGGATTA TGAACTATTA 
CAAACCAGAG AAAATGAAAA GTCTGTTCAA TTGAAGAAAG CGCTTGACGA ACGAAAAGAG 
AACGGAGCCT ATCAGCAAAG TGTTGCAAAG GCGACAGATG CTGAATGGGA GCGAAAAATG 
CAGTTTTATG AGAAGCAGTT GGAGCATGCG AATGATGAAC GGAAAAGAGA AGAGCAAAAG 
CGAACGGCTG CAGAATTCGA TCAAAGCAGG GTGGCAAGGA AATTGGCTGG GATTGAAGCG 
AATTATGAGC TGCTGCAGAA TGACTATAAA AGTATGAAGG AAGCAAGGAA GGATCTTGAA 
AGAGATCTTC AGGATGTCAT TACTGAGAAA AGGCGACTTG AAATACGTGT CGAACAGTTG 
ATGGACAGCC GGAACACCGA CGAACGGGTA TTATCGCTCT GTCAGGATGA GCTCGTGGAA 
AGTCAAGAGG AAGCAAAGTA TAAGGAAGAT GGATTGAGAG GAAAGATTGA CGGATTTAAA 
CATGAACTTG AGAATGAGAA GATGAAGACT CAAACTTTGG AGGAAAATCT TCTGGTAGCT 
GATAAGGAGA GGGGCATGCT GAAAATGGAA GTTCAAGAGC TGATGCAAAG GCATAAATGG 
GAGATTACCA ACAAAGATCA AACGCTGAAA CATCTTGAGA CTCAACTAGA CGAAATAAAG 
CAACAATCAA AGATCGAGTC GTCTGAGCAG GAATCGAATG ATAAGCAGAC GATCGCTGAT 
CTTCGAAAGA AGTTGGATCT AGAGAAAGCT CACAAGAAGG CTGTAATCAA TAAGTTAGAA 
GAGGAAATGG CAAAGCGGCA ACCATTGAAA AAGGGTGAAA AGGGTGTCAC AAAGTCTGCG 
TTGATAAAGA AAGAACGAGA GATTATGGCT CTCGAACAAG AACGCGATAC GATGAGTAAA 
AGGATTGCCG CTCTGTTTTA CGAAAATGAT AAGCAAGCCG AGCATTTCAA CATTGCGATT 
CAAGATATGC AGACTACACA AGACGCTTTA CGAGATGAGC TTAAAGAATG CAAAGAGGAG 
TTAGCCAACA GAAATGTGAA TACTCGATAT GAAGATAAAC GATCATTGGA TTCTCGAGAA 
GGGATTCCAT CTTCGTTAAG CAATCAACAT ATTCAAATGG AAGGCTGGCT ATCGCTCAGA 
GATAATACAA AGAAGTCACG GAAACCGAAG TGGACCAACT GCTTTGTTGC GCTTAACGAG 
TATTCATTCA CAATCTATGT CGACGAAAAA GCCGTGTCTG TGATTCTTTT GATTGAAGCC 
GGAGCTATGG CTCATGTTCG ACACGTCACT GCTGCTGATT TGAGAAATGT CGACGATAGT 
CAGTTACCTA AGATATTCCA TATCATGTAT GACGACGCGT CGTGCAATTC GTCACGACAC 
GCCTCCAATT CAGATCTCTC AATGATTGAA TCGTTCCGAG AAGAAAGTTG GAAGCGGCAC 
GATTTCCAAG AGCTCTCCTA TCACATACGC ACGTTTTGTG ATGTCTGCAA TAAGAAATTG 
TCTGACATTA TCCGACCGAC GCCTGCATTC GAATGCAAAA ATTGCCATTT CAAGACACAC 
AAGGATCATG TTGCTCAAGG ATCGTTGCCA ATGTGTCGAT ACAACACGGG TCTCTCTCGT 
GAACTCGTTC TTATGGCTCC ACAAACGGAT AACTGCAATA AATGGGTATC ACAACTTCGA 
CGCTTCATTG AATCATCACG ATCAGCGGCA GTTTCAGTGT CTCGTGTCTC TTCACGACGA 
CATGCTCCAG GATCGTCAAA CTCTTCCACA ATCTATCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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let-502 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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