NSMAF cDNA ORF clone, Bubalus bubalis(water buffalo)

The following NSMAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSMAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu49505 XM_025265368.1
Latest version!
Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu49505 XM_025265370.1
Latest version!
Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu49505 XM_025265369.1
Latest version!
Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu49506 XM_025265371.1
Latest version!
Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu49507 XM_025265372.1
Latest version!
Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu49508 XM_025265373.1
Latest version!
Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu49509 XM_025265374.1
Latest version!
Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu49510 XM_025265375.1
Latest version!
Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu49511 XM_025265376.1
Latest version!
Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu04644 XM_006045655.1
Latest version!
Bubalus bubalis neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu49505
    Clone ID Related Accession (Same CDS sequence) XM_025265370.1 , XM_025265369.1 , XM_025265368.1
    Accession Version XM_025265370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein FAN isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGGTAG GCGGACTGCG GGCCAGCGAG AGCTCCTTTG CCCTGCAGTT TTCGTCTTCT
    AAACCCGTGG TCCAGATAAT GCTCCAGGAC ATCCGCCGCA TTTACAAGAG GAGACACGGC
    CTCATGCCTC TGGGCTTGGA AGTGTTTTGC ACAGAAGATG ACCTGTGTTC CGACATCTAC
    CTGAAGTTCT ATGAACGTCA GGACCGAGAC GACCTCTACT TTTACATCGC TACGTACCTA
    GAGCACCACG TGCCGGAGCG CACGGCCGAG GGCTACACGC TGCAGTGGCA GCGCGGCCTC
    CTGTCCAACT ACCAGTACCT GCTGCACCTC AACTCGCTGG CCGACCGCAG CTGCAGCGAC
    CTCTCCCAGT ACCCCGTGTT CCCCTGGGTC GTCGGCGACT ACAGCAGCTC CGAGCTGGAT
    CTGTCAAATC CAGAGACCTT CCGGGATCTC AGTAAGCCAG TAGGGGCCCT CAACCAGGAG
    CGTCTGGAGA GGCTGCTGAC CCGCTACCAG GAGATGCCGG AGCCCAGGTT CATGTACGGC
    AGCCACTACT CCTCCCCAGG CTACGTGCTC TTCTACCTGG TCCGAATCGC GCCAGAGTAC
    ATGCTGTGCC TGCAGAATGG AAGGTTCGAC AACGCAGACA GAATGTTCAA CAGTATTGCA
    GAAACTTGGA AAAACTGTTT GGATGGTGCA ACCGATTTTA AAGAGCTGAT CCCGGAATTC
    TACGGTGATG ATGTCAGCTT TTTAGTCAAC AGTCTGAAGT TGGATTTGGG GAAGAGACAA
    GGAGGGCAAC TGGTTAACGA CGTAGAGCTC CCGCCTTGGG CACGGAGTCC CGAGGATTTT
    CTCCGGAAGA GCAGAGCCGC GCTGGAGAGT GAGTATGTGT CCGAGCACCT GCATGAGTGG
    ATCGACCTGG TGTTTGGCTA CAAGCAGCAG GGCCGGGATG CCGTCGGAGC CCATAATGTG
    TTTCATCCGC TGACCTATGA AGGAGGTGTG GACTTGAACA GCATCGAGGA TCCTGACGAG
    AAGGTCGCCA TGCTCACCCA GATCTTAGAG TTCGGACAGA CACCCAGACA GCTCTTTGTG
    ACACCCCATC CTCGAAGAAT CACCCCAAAG TTTAAAAACG TGTCCCAGCC CTCCAGTTAC
    AATGCCTCCA CGGCAGATTC TCCAGTCTCT CTAGGCGAAG AGTCTTTTGA AGACCTGACA
    GAAGAGAGCA GAACCCTGGC CTGGAGTAAC ATCGCCCGGC TAGAGCCCCA GGAGCAGCAC
    AGAATCCACA AAGAGGCAGT CACTGGAATA GCCGTGTCCT GCAATGGGTC GTCCATCTTC
    ACAACCTCAC AAGATTCCAC CTTGAAGATG TTTTCCAAAG AATCCAGGAT GCTACAGAGA
    AGCATATCAT TCTCAAATAT GGCTTTATCA TCCTGTTTGC TTTTACCAGG AGATGCCACT
    GTCATAAGCT CTTCCTGGGA TAATAACGTC TATTTTTATT CCATAGCATT TGGAAGATGC
    CAGGACACAT TAATGGGACA TGACGATGCT GTTAGTAAGA TCTGTTGGCA TGACAACAGG
    CTGTATTCTG CATCGTGGGA CTCTACTGTG AAGGTGTGGT CCGGGGTCCC CGCGGAGATG
    CCGTGTGCCA GGAAACAGCA CTTTGAGCTG CTGGCTGAGC TGGAGCACGA CGTCAGCGTG
    GATACGATCA GTTTGAACGC TGCCGGCACC CTGCTGGCCT CAGGCACCAA GGAAGGCACA
    GTGACCATCT GGGACCTCAC CACGGCCACC CTGTTACACC AGATTCCATG TCATGAGGGG
    ACTGTAGGCG ACACTGCTTT CAGCCCAGAC AGTCGCCACA TCCTGAGCAC AGGAGCCAAC
    AGCCATCTGA ACGTTATCGA CGTGCAGACA GGAATGCTCA TCTCTTCTCT GACTGCAGAC
    GAGCCCCAGA GGTGCTTTCT CTGGGATGGA AACTCCGTCG TATCTGGGAG TCAGTCTGGG
    GAGCTGCTCA TCTGGGACCT CCTTGGGGGA AGGGTCAGCA AGAGGATCCA GGGCCACTCA
    GGTAAGGTGA CCTGCCTCTT CTCCCTACAT GCTCCAGCGC CTCCTTTCCC ACCAGTGGAC
    ACGCTCTTCT CAGGTTGCAA GCCCCCAGGG GCCTCTGTGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025121155.1
    CDS223..2787
    Translation

    Target ORF information:

    RefSeq Version XM_025265370.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025265370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGGTAG GCGGACTGCG GGCCAGCGAG AGCTCCTTTG CCCTGCAGTT TTCGTCTTCT
    AAACCCGTGG TCCAGATAAT GCTCCAGGAC ATCCGCCGCA TTTACAAGAG GAGACACGGC
    CTCATGCCTC TGGGCTTGGA AGTGTTTTGC ACAGAAGATG ACCTGTGTTC CGACATCTAC
    CTGAAGTTCT ATGAACGTCA GGACCGAGAC GACCTCTACT TTTACATCGC TACGTACCTA
    GAGCACCACG TGCCGGAGCG CACGGCCGAG GGCTACACGC TGCAGTGGCA GCGCGGCCTC
    CTGTCCAACT ACCAGTACCT GCTGCACCTC AACTCGCTGG CCGACCGCAG CTGCAGCGAC
    CTCTCCCAGT ACCCCGTGTT CCCCTGGGTC GTCGGCGACT ACAGCAGCTC CGAGCTGGAT
    CTGTCAAATC CAGAGACCTT CCGGGATCTC AGTAAGCCAG TAGGGGCCCT CAACCAGGAG
    CGTCTGGAGA GGCTGCTGAC CCGCTACCAG GAGATGCCGG AGCCCAGGTT CATGTACGGC
    AGCCACTACT CCTCCCCAGG CTACGTGCTC TTCTACCTGG TCCGAATCGC GCCAGAGTAC
    ATGCTGTGCC TGCAGAATGG AAGGTTCGAC AACGCAGACA GAATGTTCAA CAGTATTGCA
    GAAACTTGGA AAAACTGTTT GGATGGTGCA ACCGATTTTA AAGAGCTGAT CCCGGAATTC
    TACGGTGATG ATGTCAGCTT TTTAGTCAAC AGTCTGAAGT TGGATTTGGG GAAGAGACAA
    GGAGGGCAAC TGGTTAACGA CGTAGAGCTC CCGCCTTGGG CACGGAGTCC CGAGGATTTT
    CTCCGGAAGA GCAGAGCCGC GCTGGAGAGT GAGTATGTGT CCGAGCACCT GCATGAGTGG
    ATCGACCTGG TGTTTGGCTA CAAGCAGCAG GGCCGGGATG CCGTCGGAGC CCATAATGTG
    TTTCATCCGC TGACCTATGA AGGAGGTGTG GACTTGAACA GCATCGAGGA TCCTGACGAG
    AAGGTCGCCA TGCTCACCCA GATCTTAGAG TTCGGACAGA CACCCAGACA GCTCTTTGTG
    ACACCCCATC CTCGAAGAAT CACCCCAAAG TTTAAAAACG TGTCCCAGCC CTCCAGTTAC
    AATGCCTCCA CGGCAGATTC TCCAGTCTCT CTAGGCGAAG AGTCTTTTGA AGACCTGACA
    GAAGAGAGCA GAACCCTGGC CTGGAGTAAC ATCGCCCGGC TAGAGCCCCA GGAGCAGCAC
    AGAATCCACA AAGAGGCAGT CACTGGAATA GCCGTGTCCT GCAATGGGTC GTCCATCTTC
    ACAACCTCAC AAGATTCCAC CTTGAAGATG TTTTCCAAAG AATCCAGGAT GCTACAGAGA
    AGCATATCAT TCTCAAATAT GGCTTTATCA TCCTGTTTGC TTTTACCAGG AGATGCCACT
    GTCATAAGCT CTTCCTGGGA TAATAACGTC TATTTTTATT CCATAGCATT TGGAAGATGC
    CAGGACACAT TAATGGGACA TGACGATGCT GTTAGTAAGA TCTGTTGGCA TGACAACAGG
    CTGTATTCTG CATCGTGGGA CTCTACTGTG AAGGTGTGGT CCGGGGTCCC CGCGGAGATG
    CCGTGTGCCA GGAAACAGCA CTTTGAGCTG CTGGCTGAGC TGGAGCACGA CGTCAGCGTG
    GATACGATCA GTTTGAACGC TGCCGGCACC CTGCTGGCCT CAGGCACCAA GGAAGGCACA
    GTGACCATCT GGGACCTCAC CACGGCCACC CTGTTACACC AGATTCCATG TCATGAGGGG
    ACTGTAGGCG ACACTGCTTT CAGCCCAGAC AGTCGCCACA TCCTGAGCAC AGGAGCCAAC
    AGCCATCTGA ACGTTATCGA CGTGCAGACA GGAATGCTCA TCTCTTCTCT GACTGCAGAC
    GAGCCCCAGA GGTGCTTTCT CTGGGATGGA AACTCCGTCG TATCTGGGAG TCAGTCTGGG
    GAGCTGCTCA TCTGGGACCT CCTTGGGGGA AGGGTCAGCA AGAGGATCCA GGGCCACTCA
    GGTAAGGTGA CCTGCCTCTT CTCCCTACAT GCTCCAGCGC CTCCTTTCCC ACCAGTGGAC
    ACGCTCTTCT CAGGTTGCAA GCCCCCAGGG GCCTCTGTGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49505
    Clone ID Related Accession (Same CDS sequence) XM_025265370.1 , XM_025265369.1 , XM_025265368.1
    Accession Version XM_025265369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein FAN isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGGTAG GCGGACTGCG GGCCAGCGAG AGCTCCTTTG CCCTGCAGTT TTCGTCTTCT
    AAACCCGTGG TCCAGATAAT GCTCCAGGAC ATCCGCCGCA TTTACAAGAG GAGACACGGC
    CTCATGCCTC TGGGCTTGGA AGTGTTTTGC ACAGAAGATG ACCTGTGTTC CGACATCTAC
    CTGAAGTTCT ATGAACGTCA GGACCGAGAC GACCTCTACT TTTACATCGC TACGTACCTA
    GAGCACCACG TGCCGGAGCG CACGGCCGAG GGCTACACGC TGCAGTGGCA GCGCGGCCTC
    CTGTCCAACT ACCAGTACCT GCTGCACCTC AACTCGCTGG CCGACCGCAG CTGCAGCGAC
    CTCTCCCAGT ACCCCGTGTT CCCCTGGGTC GTCGGCGACT ACAGCAGCTC CGAGCTGGAT
    CTGTCAAATC CAGAGACCTT CCGGGATCTC AGTAAGCCAG TAGGGGCCCT CAACCAGGAG
    CGTCTGGAGA GGCTGCTGAC CCGCTACCAG GAGATGCCGG AGCCCAGGTT CATGTACGGC
    AGCCACTACT CCTCCCCAGG CTACGTGCTC TTCTACCTGG TCCGAATCGC GCCAGAGTAC
    ATGCTGTGCC TGCAGAATGG AAGGTTCGAC AACGCAGACA GAATGTTCAA CAGTATTGCA
    GAAACTTGGA AAAACTGTTT GGATGGTGCA ACCGATTTTA AAGAGCTGAT CCCGGAATTC
    TACGGTGATG ATGTCAGCTT TTTAGTCAAC AGTCTGAAGT TGGATTTGGG GAAGAGACAA
    GGAGGGCAAC TGGTTAACGA CGTAGAGCTC CCGCCTTGGG CACGGAGTCC CGAGGATTTT
    CTCCGGAAGA GCAGAGCCGC GCTGGAGAGT GAGTATGTGT CCGAGCACCT GCATGAGTGG
    ATCGACCTGG TGTTTGGCTA CAAGCAGCAG GGCCGGGATG CCGTCGGAGC CCATAATGTG
    TTTCATCCGC TGACCTATGA AGGAGGTGTG GACTTGAACA GCATCGAGGA TCCTGACGAG
    AAGGTCGCCA TGCTCACCCA GATCTTAGAG TTCGGACAGA CACCCAGACA GCTCTTTGTG
    ACACCCCATC CTCGAAGAAT CACCCCAAAG TTTAAAAACG TGTCCCAGCC CTCCAGTTAC
    AATGCCTCCA CGGCAGATTC TCCAGTCTCT CTAGGCGAAG AGTCTTTTGA AGACCTGACA
    GAAGAGAGCA GAACCCTGGC CTGGAGTAAC ATCGCCCGGC TAGAGCCCCA GGAGCAGCAC
    AGAATCCACA AAGAGGCAGT CACTGGAATA GCCGTGTCCT GCAATGGGTC GTCCATCTTC
    ACAACCTCAC AAGATTCCAC CTTGAAGATG TTTTCCAAAG AATCCAGGAT GCTACAGAGA
    AGCATATCAT TCTCAAATAT GGCTTTATCA TCCTGTTTGC TTTTACCAGG AGATGCCACT
    GTCATAAGCT CTTCCTGGGA TAATAACGTC TATTTTTATT CCATAGCATT TGGAAGATGC
    CAGGACACAT TAATGGGACA TGACGATGCT GTTAGTAAGA TCTGTTGGCA TGACAACAGG
    CTGTATTCTG CATCGTGGGA CTCTACTGTG AAGGTGTGGT CCGGGGTCCC CGCGGAGATG
    CCGTGTGCCA GGAAACAGCA CTTTGAGCTG CTGGCTGAGC TGGAGCACGA CGTCAGCGTG
    GATACGATCA GTTTGAACGC TGCCGGCACC CTGCTGGCCT CAGGCACCAA GGAAGGCACA
    GTGACCATCT GGGACCTCAC CACGGCCACC CTGTTACACC AGATTCCATG TCATGAGGGG
    ACTGTAGGCG ACACTGCTTT CAGCCCAGAC AGTCGCCACA TCCTGAGCAC AGGAGCCAAC
    AGCCATCTGA ACGTTATCGA CGTGCAGACA GGAATGCTCA TCTCTTCTCT GACTGCAGAC
    GAGCCCCAGA GGTGCTTTCT CTGGGATGGA AACTCCGTCG TATCTGGGAG TCAGTCTGGG
    GAGCTGCTCA TCTGGGACCT CCTTGGGGGA AGGGTCAGCA AGAGGATCCA GGGCCACTCA
    GGTAAGGTGA CCTGCCTCTT CTCCCTACAT GCTCCAGCGC CTCCTTTCCC ACCAGTGGAC
    ACGCTCTTCT CAGGTTGCAA GCCCCCAGGG GCCTCTGTGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025121154.1
    CDS489..3053
    Translation

    Target ORF information:

    RefSeq Version XM_025265369.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025265369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGGTAG GCGGACTGCG GGCCAGCGAG AGCTCCTTTG CCCTGCAGTT TTCGTCTTCT
    AAACCCGTGG TCCAGATAAT GCTCCAGGAC ATCCGCCGCA TTTACAAGAG GAGACACGGC
    CTCATGCCTC TGGGCTTGGA AGTGTTTTGC ACAGAAGATG ACCTGTGTTC CGACATCTAC
    CTGAAGTTCT ATGAACGTCA GGACCGAGAC GACCTCTACT TTTACATCGC TACGTACCTA
    GAGCACCACG TGCCGGAGCG CACGGCCGAG GGCTACACGC TGCAGTGGCA GCGCGGCCTC
    CTGTCCAACT ACCAGTACCT GCTGCACCTC AACTCGCTGG CCGACCGCAG CTGCAGCGAC
    CTCTCCCAGT ACCCCGTGTT CCCCTGGGTC GTCGGCGACT ACAGCAGCTC CGAGCTGGAT
    CTGTCAAATC CAGAGACCTT CCGGGATCTC AGTAAGCCAG TAGGGGCCCT CAACCAGGAG
    CGTCTGGAGA GGCTGCTGAC CCGCTACCAG GAGATGCCGG AGCCCAGGTT CATGTACGGC
    AGCCACTACT CCTCCCCAGG CTACGTGCTC TTCTACCTGG TCCGAATCGC GCCAGAGTAC
    ATGCTGTGCC TGCAGAATGG AAGGTTCGAC AACGCAGACA GAATGTTCAA CAGTATTGCA
    GAAACTTGGA AAAACTGTTT GGATGGTGCA ACCGATTTTA AAGAGCTGAT CCCGGAATTC
    TACGGTGATG ATGTCAGCTT TTTAGTCAAC AGTCTGAAGT TGGATTTGGG GAAGAGACAA
    GGAGGGCAAC TGGTTAACGA CGTAGAGCTC CCGCCTTGGG CACGGAGTCC CGAGGATTTT
    CTCCGGAAGA GCAGAGCCGC GCTGGAGAGT GAGTATGTGT CCGAGCACCT GCATGAGTGG
    ATCGACCTGG TGTTTGGCTA CAAGCAGCAG GGCCGGGATG CCGTCGGAGC CCATAATGTG
    TTTCATCCGC TGACCTATGA AGGAGGTGTG GACTTGAACA GCATCGAGGA TCCTGACGAG
    AAGGTCGCCA TGCTCACCCA GATCTTAGAG TTCGGACAGA CACCCAGACA GCTCTTTGTG
    ACACCCCATC CTCGAAGAAT CACCCCAAAG TTTAAAAACG TGTCCCAGCC CTCCAGTTAC
    AATGCCTCCA CGGCAGATTC TCCAGTCTCT CTAGGCGAAG AGTCTTTTGA AGACCTGACA
    GAAGAGAGCA GAACCCTGGC CTGGAGTAAC ATCGCCCGGC TAGAGCCCCA GGAGCAGCAC
    AGAATCCACA AAGAGGCAGT CACTGGAATA GCCGTGTCCT GCAATGGGTC GTCCATCTTC
    ACAACCTCAC AAGATTCCAC CTTGAAGATG TTTTCCAAAG AATCCAGGAT GCTACAGAGA
    AGCATATCAT TCTCAAATAT GGCTTTATCA TCCTGTTTGC TTTTACCAGG AGATGCCACT
    GTCATAAGCT CTTCCTGGGA TAATAACGTC TATTTTTATT CCATAGCATT TGGAAGATGC
    CAGGACACAT TAATGGGACA TGACGATGCT GTTAGTAAGA TCTGTTGGCA TGACAACAGG
    CTGTATTCTG CATCGTGGGA CTCTACTGTG AAGGTGTGGT CCGGGGTCCC CGCGGAGATG
    CCGTGTGCCA GGAAACAGCA CTTTGAGCTG CTGGCTGAGC TGGAGCACGA CGTCAGCGTG
    GATACGATCA GTTTGAACGC TGCCGGCACC CTGCTGGCCT CAGGCACCAA GGAAGGCACA
    GTGACCATCT GGGACCTCAC CACGGCCACC CTGTTACACC AGATTCCATG TCATGAGGGG
    ACTGTAGGCG ACACTGCTTT CAGCCCAGAC AGTCGCCACA TCCTGAGCAC AGGAGCCAAC
    AGCCATCTGA ACGTTATCGA CGTGCAGACA GGAATGCTCA TCTCTTCTCT GACTGCAGAC
    GAGCCCCAGA GGTGCTTTCT CTGGGATGGA AACTCCGTCG TATCTGGGAG TCAGTCTGGG
    GAGCTGCTCA TCTGGGACCT CCTTGGGGGA AGGGTCAGCA AGAGGATCCA GGGCCACTCA
    GGTAAGGTGA CCTGCCTCTT CTCCCTACAT GCTCCAGCGC CTCCTTTCCC ACCAGTGGAC
    ACGCTCTTCT CAGGTTGCAA GCCCCCAGGG GCCTCTGTGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49505
    Clone ID Related Accession (Same CDS sequence) XM_025265370.1 , XM_025265369.1 , XM_025265368.1
    Accession Version XM_025265368.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein FAN isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGGTAG GCGGACTGCG GGCCAGCGAG AGCTCCTTTG CCCTGCAGTT TTCGTCTTCT
    AAACCCGTGG TCCAGATAAT GCTCCAGGAC ATCCGCCGCA TTTACAAGAG GAGACACGGC
    CTCATGCCTC TGGGCTTGGA AGTGTTTTGC ACAGAAGATG ACCTGTGTTC CGACATCTAC
    CTGAAGTTCT ATGAACGTCA GGACCGAGAC GACCTCTACT TTTACATCGC TACGTACCTA
    GAGCACCACG TGCCGGAGCG CACGGCCGAG GGCTACACGC TGCAGTGGCA GCGCGGCCTC
    CTGTCCAACT ACCAGTACCT GCTGCACCTC AACTCGCTGG CCGACCGCAG CTGCAGCGAC
    CTCTCCCAGT ACCCCGTGTT CCCCTGGGTC GTCGGCGACT ACAGCAGCTC CGAGCTGGAT
    CTGTCAAATC CAGAGACCTT CCGGGATCTC AGTAAGCCAG TAGGGGCCCT CAACCAGGAG
    CGTCTGGAGA GGCTGCTGAC CCGCTACCAG GAGATGCCGG AGCCCAGGTT CATGTACGGC
    AGCCACTACT CCTCCCCAGG CTACGTGCTC TTCTACCTGG TCCGAATCGC GCCAGAGTAC
    ATGCTGTGCC TGCAGAATGG AAGGTTCGAC AACGCAGACA GAATGTTCAA CAGTATTGCA
    GAAACTTGGA AAAACTGTTT GGATGGTGCA ACCGATTTTA AAGAGCTGAT CCCGGAATTC
    TACGGTGATG ATGTCAGCTT TTTAGTCAAC AGTCTGAAGT TGGATTTGGG GAAGAGACAA
    GGAGGGCAAC TGGTTAACGA CGTAGAGCTC CCGCCTTGGG CACGGAGTCC CGAGGATTTT
    CTCCGGAAGA GCAGAGCCGC GCTGGAGAGT GAGTATGTGT CCGAGCACCT GCATGAGTGG
    ATCGACCTGG TGTTTGGCTA CAAGCAGCAG GGCCGGGATG CCGTCGGAGC CCATAATGTG
    TTTCATCCGC TGACCTATGA AGGAGGTGTG GACTTGAACA GCATCGAGGA TCCTGACGAG
    AAGGTCGCCA TGCTCACCCA GATCTTAGAG TTCGGACAGA CACCCAGACA GCTCTTTGTG
    ACACCCCATC CTCGAAGAAT CACCCCAAAG TTTAAAAACG TGTCCCAGCC CTCCAGTTAC
    AATGCCTCCA CGGCAGATTC TCCAGTCTCT CTAGGCGAAG AGTCTTTTGA AGACCTGACA
    GAAGAGAGCA GAACCCTGGC CTGGAGTAAC ATCGCCCGGC TAGAGCCCCA GGAGCAGCAC
    AGAATCCACA AAGAGGCAGT CACTGGAATA GCCGTGTCCT GCAATGGGTC GTCCATCTTC
    ACAACCTCAC AAGATTCCAC CTTGAAGATG TTTTCCAAAG AATCCAGGAT GCTACAGAGA
    AGCATATCAT TCTCAAATAT GGCTTTATCA TCCTGTTTGC TTTTACCAGG AGATGCCACT
    GTCATAAGCT CTTCCTGGGA TAATAACGTC TATTTTTATT CCATAGCATT TGGAAGATGC
    CAGGACACAT TAATGGGACA TGACGATGCT GTTAGTAAGA TCTGTTGGCA TGACAACAGG
    CTGTATTCTG CATCGTGGGA CTCTACTGTG AAGGTGTGGT CCGGGGTCCC CGCGGAGATG
    CCGTGTGCCA GGAAACAGCA CTTTGAGCTG CTGGCTGAGC TGGAGCACGA CGTCAGCGTG
    GATACGATCA GTTTGAACGC TGCCGGCACC CTGCTGGCCT CAGGCACCAA GGAAGGCACA
    GTGACCATCT GGGACCTCAC CACGGCCACC CTGTTACACC AGATTCCATG TCATGAGGGG
    ACTGTAGGCG ACACTGCTTT CAGCCCAGAC AGTCGCCACA TCCTGAGCAC AGGAGCCAAC
    AGCCATCTGA ACGTTATCGA CGTGCAGACA GGAATGCTCA TCTCTTCTCT GACTGCAGAC
    GAGCCCCAGA GGTGCTTTCT CTGGGATGGA AACTCCGTCG TATCTGGGAG TCAGTCTGGG
    GAGCTGCTCA TCTGGGACCT CCTTGGGGGA AGGGTCAGCA AGAGGATCCA GGGCCACTCA
    GGTAAGGTGA CCTGCCTCTT CTCCCTACAT GCTCCAGCGC CTCCTTTCCC ACCAGTGGAC
    ACGCTCTTCT CAGGTTGCAA GCCCCCAGGG GCCTCTGTGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025121153.1
    CDS1499..4063
    Translation

    Target ORF information:

    RefSeq Version XM_025265368.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025265368.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGGTAG GCGGACTGCG GGCCAGCGAG AGCTCCTTTG CCCTGCAGTT TTCGTCTTCT
    AAACCCGTGG TCCAGATAAT GCTCCAGGAC ATCCGCCGCA TTTACAAGAG GAGACACGGC
    CTCATGCCTC TGGGCTTGGA AGTGTTTTGC ACAGAAGATG ACCTGTGTTC CGACATCTAC
    CTGAAGTTCT ATGAACGTCA GGACCGAGAC GACCTCTACT TTTACATCGC TACGTACCTA
    GAGCACCACG TGCCGGAGCG CACGGCCGAG GGCTACACGC TGCAGTGGCA GCGCGGCCTC
    CTGTCCAACT ACCAGTACCT GCTGCACCTC AACTCGCTGG CCGACCGCAG CTGCAGCGAC
    CTCTCCCAGT ACCCCGTGTT CCCCTGGGTC GTCGGCGACT ACAGCAGCTC CGAGCTGGAT
    CTGTCAAATC CAGAGACCTT CCGGGATCTC AGTAAGCCAG TAGGGGCCCT CAACCAGGAG
    CGTCTGGAGA GGCTGCTGAC CCGCTACCAG GAGATGCCGG AGCCCAGGTT CATGTACGGC
    AGCCACTACT CCTCCCCAGG CTACGTGCTC TTCTACCTGG TCCGAATCGC GCCAGAGTAC
    ATGCTGTGCC TGCAGAATGG AAGGTTCGAC AACGCAGACA GAATGTTCAA CAGTATTGCA
    GAAACTTGGA AAAACTGTTT GGATGGTGCA ACCGATTTTA AAGAGCTGAT CCCGGAATTC
    TACGGTGATG ATGTCAGCTT TTTAGTCAAC AGTCTGAAGT TGGATTTGGG GAAGAGACAA
    GGAGGGCAAC TGGTTAACGA CGTAGAGCTC CCGCCTTGGG CACGGAGTCC CGAGGATTTT
    CTCCGGAAGA GCAGAGCCGC GCTGGAGAGT GAGTATGTGT CCGAGCACCT GCATGAGTGG
    ATCGACCTGG TGTTTGGCTA CAAGCAGCAG GGCCGGGATG CCGTCGGAGC CCATAATGTG
    TTTCATCCGC TGACCTATGA AGGAGGTGTG GACTTGAACA GCATCGAGGA TCCTGACGAG
    AAGGTCGCCA TGCTCACCCA GATCTTAGAG TTCGGACAGA CACCCAGACA GCTCTTTGTG
    ACACCCCATC CTCGAAGAAT CACCCCAAAG TTTAAAAACG TGTCCCAGCC CTCCAGTTAC
    AATGCCTCCA CGGCAGATTC TCCAGTCTCT CTAGGCGAAG AGTCTTTTGA AGACCTGACA
    GAAGAGAGCA GAACCCTGGC CTGGAGTAAC ATCGCCCGGC TAGAGCCCCA GGAGCAGCAC
    AGAATCCACA AAGAGGCAGT CACTGGAATA GCCGTGTCCT GCAATGGGTC GTCCATCTTC
    ACAACCTCAC AAGATTCCAC CTTGAAGATG TTTTCCAAAG AATCCAGGAT GCTACAGAGA
    AGCATATCAT TCTCAAATAT GGCTTTATCA TCCTGTTTGC TTTTACCAGG AGATGCCACT
    GTCATAAGCT CTTCCTGGGA TAATAACGTC TATTTTTATT CCATAGCATT TGGAAGATGC
    CAGGACACAT TAATGGGACA TGACGATGCT GTTAGTAAGA TCTGTTGGCA TGACAACAGG
    CTGTATTCTG CATCGTGGGA CTCTACTGTG AAGGTGTGGT CCGGGGTCCC CGCGGAGATG
    CCGTGTGCCA GGAAACAGCA CTTTGAGCTG CTGGCTGAGC TGGAGCACGA CGTCAGCGTG
    GATACGATCA GTTTGAACGC TGCCGGCACC CTGCTGGCCT CAGGCACCAA GGAAGGCACA
    GTGACCATCT GGGACCTCAC CACGGCCACC CTGTTACACC AGATTCCATG TCATGAGGGG
    ACTGTAGGCG ACACTGCTTT CAGCCCAGAC AGTCGCCACA TCCTGAGCAC AGGAGCCAAC
    AGCCATCTGA ACGTTATCGA CGTGCAGACA GGAATGCTCA TCTCTTCTCT GACTGCAGAC
    GAGCCCCAGA GGTGCTTTCT CTGGGATGGA AACTCCGTCG TATCTGGGAG TCAGTCTGGG
    GAGCTGCTCA TCTGGGACCT CCTTGGGGGA AGGGTCAGCA AGAGGATCCA GGGCCACTCA
    GGTAAGGTGA CCTGCCTCTT CTCCCTACAT GCTCCAGCGC CTCCTTTCCC ACCAGTGGAC
    ACGCTCTTCT CAGGTTGCAA GCCCCCAGGG GCCTCTGTGC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49506
    Clone ID Related Accession (Same CDS sequence) XM_025265371.1
    Accession Version XM_025265371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein FAN isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGGTAG GCGGACTGCG GGCCAGCGAG AGCTCCTTTG CCCTGCAGTT TTCGTCTTCT
    AAACCCGTGG TCCAGATAAT GCTCCAGGAC ATCCGCCGCA TTTACAAGAG GAGACACGGC
    CTCATGCCTC TGGGCTTGGA AGTGTTTTGC ACAGAAGATG ACCTGTGTTC CGACATCTAC
    CTGAAGTTCT ATGAACGTCA GGACCGAGAC GACCTCTACT TTTACATCGC TACGTACCTA
    GAGCACCACG TGCCGGAGCG CACGGCCGAG GGCTACACGC TGCAGTGGCA GCGCGGCCTC
    CTGTCCAACT ACCAGTACCT GCTGCACCTC AACTCGCTGG CCGACCGCAG CTGCAGCGAC
    CTCTCCCAGT ACCCCGTGTT CCCCTGGGTC GTCGGCGACT ACAGCAGCTC CGAGCTGGAT
    CTGTCAAATC CAGAGACCTT CCGGGATCTC AGTAAGCCAG TAGGGGCCCT CAACCAGGAG
    CGTCTGGAGA GGCTGCTGAC CCGCTACCAG GAGATGCCGG AGCCCAGGTT CATGTACGGC
    AGCCACTACT CCTCCCCAGG CTACGTGCTC TTCTACCTGG TCCGAATCGC GCCAGAGTAC
    ATGCTGTGCC TGCAGAATGG AAGGTTCGAC AACGCAGACA GAATGTTCAA CAGTATTGCA
    GAAACTTGGA AAAACTGTTT GGATGGTGCA ACCGATTTTA AAGAGCTGAT CCCGGAATTC
    TACGGTGATG ATGTCAGCTT TTTAGTCAAC AGTCTGAAGT TGGATTTGGG GAAGAGACAA
    GGAGGGCAAC TGGTTAACGA CGTAGAGCTC CCGCCTTGGG CACGGAGTCC CGAGGATTTT
    CTCCGGAAGA GCAGAGCCGC GCTGGAGAGT GAGTATGTGT CCGAGCACCT GCATGAGTGG
    ATCGACCTGG TGTTTGGCTA CAAGCAGCAG GGCCGGGATG CCGTCGGAGC CCATAATGTG
    TTTCATCCGC TGACCTATGA AGGAGGTGTG GACTTGAACA GCATCGAGGA TCCTGACGAG
    AAGGTCGCCA TGCTCACCCA GATCTTAGAG TTCGGACAGA CACCCAGACA GCTCTTTGTG
    ACACCCCATC CTCGAAGAAT CACCCCAAAG TTTAAAAACG TGTCCCAGCC CTCCAGTTAC
    AATGCCTCCA CGGCAGATTC TCCAGTCTCT CTAGGCGAAG AGTCTTTTGA AGACCTGACA
    GAAGAGAGCA GAACCCTGGC CTGGAGTAAC ATCGCCCGGC TAGAGCCCCA GGAGCAGCAC
    AGAATCCACA AAGAGGCAGT CACTGGAATA GCCGTGTCCT GCAATGGGTC GTCCATCTTC
    ACAACCTCAC AAGATTCCAC CTTGAAGATG TTTTCCAAAG AATCCAGGAT GCTACAGAGA
    AGCATATCAT TCTCAAATAT GGCTTTATCA TCCTGTTTGC TTTTACCAGG AGATGCCACT
    GTCATAAGCT CTTCCTGGGA TAATAACGTC TATTTTTATT CCATAGCATT TGGAAGATGC
    CAGGACACAT TAATGGGACA TGACGATGCT GTTAGTAAGA TCTGTTGGCA TGACAACAGG
    CTGTATTCTG CATCGTGGGA CTCTACTGTG AAGGTGTGGT CCGGGGTCCC CGCGGAGATG
    CCGTGTGCCA GGAAACAGCA CTTTGAGCTG CTGGCTGAGC TGGAGCACGA CGTCAGCGTG
    GATACGATCA GTTTGAACGC TGCCGGCACC CTGCTGGCCT CAGGCACCAA GGAAGGCACA
    GTGACCATCT GGGACCTCAC CACGGCCACC CTGTTACACC AGATTCCATG TCATGAGGGG
    ACTGTAGGCG ACACTGCTTT CAGCCCAGAC AGTCGCCACA TCCTGAGCAC AGGAGCCAAC
    AGCCATCTGA ACGTTATCGA CGTGCAGACA GGAATGCTCA TCTCTTCTCT GACTGCAGAC
    GAGCCCCAGA GGTGCTTTCT CTGGGATGGA AACTCCGTCG TATCTGGGAG TCAGTCTGGG
    GAGCTGCTCA TCTGGGACCT CCTTGGGGGA AGGGTCAGCA AGAGGATCCA GGGCCACTCA
    GGCGCCGTGA CGTGCATGTG GATGAGCGAG CAGTGTAGCA GCGTCATCAC AGGAGGGGAA
    GACAGACAAA TTATGTTCTG GAAGTCGCAG TATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025121156.1
    CDS1498..4053
    Translation

    Target ORF information:

    RefSeq Version XM_025265371.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025265371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGGTAG GCGGACTGCG GGCCAGCGAG AGCTCCTTTG CCCTGCAGTT TTCGTCTTCT
    AAACCCGTGG TCCAGATAAT GCTCCAGGAC ATCCGCCGCA TTTACAAGAG GAGACACGGC
    CTCATGCCTC TGGGCTTGGA AGTGTTTTGC ACAGAAGATG ACCTGTGTTC CGACATCTAC
    CTGAAGTTCT ATGAACGTCA GGACCGAGAC GACCTCTACT TTTACATCGC TACGTACCTA
    GAGCACCACG TGCCGGAGCG CACGGCCGAG GGCTACACGC TGCAGTGGCA GCGCGGCCTC
    CTGTCCAACT ACCAGTACCT GCTGCACCTC AACTCGCTGG CCGACCGCAG CTGCAGCGAC
    CTCTCCCAGT ACCCCGTGTT CCCCTGGGTC GTCGGCGACT ACAGCAGCTC CGAGCTGGAT
    CTGTCAAATC CAGAGACCTT CCGGGATCTC AGTAAGCCAG TAGGGGCCCT CAACCAGGAG
    CGTCTGGAGA GGCTGCTGAC CCGCTACCAG GAGATGCCGG AGCCCAGGTT CATGTACGGC
    AGCCACTACT CCTCCCCAGG CTACGTGCTC TTCTACCTGG TCCGAATCGC GCCAGAGTAC
    ATGCTGTGCC TGCAGAATGG AAGGTTCGAC AACGCAGACA GAATGTTCAA CAGTATTGCA
    GAAACTTGGA AAAACTGTTT GGATGGTGCA ACCGATTTTA AAGAGCTGAT CCCGGAATTC
    TACGGTGATG ATGTCAGCTT TTTAGTCAAC AGTCTGAAGT TGGATTTGGG GAAGAGACAA
    GGAGGGCAAC TGGTTAACGA CGTAGAGCTC CCGCCTTGGG CACGGAGTCC CGAGGATTTT
    CTCCGGAAGA GCAGAGCCGC GCTGGAGAGT GAGTATGTGT CCGAGCACCT GCATGAGTGG
    ATCGACCTGG TGTTTGGCTA CAAGCAGCAG GGCCGGGATG CCGTCGGAGC CCATAATGTG
    TTTCATCCGC TGACCTATGA AGGAGGTGTG GACTTGAACA GCATCGAGGA TCCTGACGAG
    AAGGTCGCCA TGCTCACCCA GATCTTAGAG TTCGGACAGA CACCCAGACA GCTCTTTGTG
    ACACCCCATC CTCGAAGAAT CACCCCAAAG TTTAAAAACG TGTCCCAGCC CTCCAGTTAC
    AATGCCTCCA CGGCAGATTC TCCAGTCTCT CTAGGCGAAG AGTCTTTTGA AGACCTGACA
    GAAGAGAGCA GAACCCTGGC CTGGAGTAAC ATCGCCCGGC TAGAGCCCCA GGAGCAGCAC
    AGAATCCACA AAGAGGCAGT CACTGGAATA GCCGTGTCCT GCAATGGGTC GTCCATCTTC
    ACAACCTCAC AAGATTCCAC CTTGAAGATG TTTTCCAAAG AATCCAGGAT GCTACAGAGA
    AGCATATCAT TCTCAAATAT GGCTTTATCA TCCTGTTTGC TTTTACCAGG AGATGCCACT
    GTCATAAGCT CTTCCTGGGA TAATAACGTC TATTTTTATT CCATAGCATT TGGAAGATGC
    CAGGACACAT TAATGGGACA TGACGATGCT GTTAGTAAGA TCTGTTGGCA TGACAACAGG
    CTGTATTCTG CATCGTGGGA CTCTACTGTG AAGGTGTGGT CCGGGGTCCC CGCGGAGATG
    CCGTGTGCCA GGAAACAGCA CTTTGAGCTG CTGGCTGAGC TGGAGCACGA CGTCAGCGTG
    GATACGATCA GTTTGAACGC TGCCGGCACC CTGCTGGCCT CAGGCACCAA GGAAGGCACA
    GTGACCATCT GGGACCTCAC CACGGCCACC CTGTTACACC AGATTCCATG TCATGAGGGG
    ACTGTAGGCG ACACTGCTTT CAGCCCAGAC AGTCGCCACA TCCTGAGCAC AGGAGCCAAC
    AGCCATCTGA ACGTTATCGA CGTGCAGACA GGAATGCTCA TCTCTTCTCT GACTGCAGAC
    GAGCCCCAGA GGTGCTTTCT CTGGGATGGA AACTCCGTCG TATCTGGGAG TCAGTCTGGG
    GAGCTGCTCA TCTGGGACCT CCTTGGGGGA AGGGTCAGCA AGAGGATCCA GGGCCACTCA
    GGCGCCGTGA CGTGCATGTG GATGAGCGAG CAGTGTAGCA GCGTCATCAC AGGAGGGGAA
    GACAGACAAA TTATGTTCTG GAAGTCGCAG TATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49507
    Clone ID Related Accession (Same CDS sequence) XM_025265372.1
    Accession Version XM_025265372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein FAN isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGAAAC CCGTGGTCCA GATAATGCTC CAGGACATCC GCCGCATTTA CAAGAGGAGA
    CACGGCCTCA TGCCTCTGGG CTTGGAAGTG TTTTGCACAG AAGATGACCT GTGTTCCGAC
    ATCTACCTGA AGTTCTATGA ACGTCAGGAC CGAGACGACC TCTACTTTTA CATCGCTACG
    TACCTAGAGC ACCACGTGCC GGAGCGCACG GCCGAGGGCT ACACGCTGCA GTGGCAGCGC
    GGCCTCCTGT CCAACTACCA GTACCTGCTG CACCTCAACT CGCTGGCCGA CCGCAGCTGC
    AGCGACCTCT CCCAGTACCC CGTGTTCCCC TGGGTCGTCG GCGACTACAG CAGCTCCGAG
    CTGGATCTGT CAAATCCAGA GACCTTCCGG GATCTCAGTA AGCCAGTAGG GGCCCTCAAC
    CAGGAGCGTC TGGAGAGGCT GCTGACCCGC TACCAGGAGA TGCCGGAGCC CAGGTTCATG
    TACGGCAGCC ACTACTCCTC CCCAGGCTAC GTGCTCTTCT ACCTGGTCCG AATCGCGCCA
    GAGTACATGC TGTGCCTGCA GAATGGAAGG TTCGACAACG CAGACAGAAT GTTCAACAGT
    ATTGCAGAAA CTTGGAAAAA CTGTTTGGAT GGTGCAACCG ATTTTAAAGA GCTGATCCCG
    GAATTCTACG GTGATGATGT CAGCTTTTTA GTCAACAGTC TGAAGTTGGA TTTGGGGAAG
    AGACAAGGAG GGCAACTGGT TAACGACGTA GAGCTCCCGC CTTGGGCACG GAGTCCCGAG
    GATTTTCTCC GGAAGAGCAG AGCCGCGCTG GAGAGTGAGT ATGTGTCCGA GCACCTGCAT
    GAGTGGATCG ACCTGGTGTT TGGCTACAAG CAGCAGGGCC GGGATGCCGT CGGAGCCCAT
    AATGTGTTTC ATCCGCTGAC CTATGAAGGA GGTGTGGACT TGAACAGCAT CGAGGATCCT
    GACGAGAAGG TCGCCATGCT CACCCAGATC TTAGAGTTCG GACAGACACC CAGACAGCTC
    TTTGTGACAC CCCATCCTCG AAGAATCACC CCAAAGTTTA AAAACGTGTC CCAGCCCTCC
    AGTTACAATG CCTCCACGGC AGATTCTCCA GTCTCTCTAG GCGAAGAGTC TTTTGAAGAC
    CTGACAGAAG AGAGCAGAAC CCTGGCCTGG AGTAACATCG CCCGGCTAGA GCCCCAGGAG
    CAGCACAGAA TCCACAAAGA GGCAGTCACT GGAATAGCCG TGTCCTGCAA TGGGTCGTCC
    ATCTTCACAA CCTCACAAGA TTCCACCTTG AAGATGTTTT CCAAAGAATC CAGGATGCTA
    CAGAGAAGCA TATCATTCTC AAATATGGCT TTATCATCCT GTTTGCTTTT ACCAGGAGAT
    GCCACTGTCA TAAGCTCTTC CTGGGATAAT AACGTCTATT TTTATTCCAT AGCATTTGGA
    AGATGCCAGG ACACATTAAT GGGACATGAC GATGCTGTTA GTAAGATCTG TTGGCATGAC
    AACAGGCTGT ATTCTGCATC GTGGGACTCT ACTGTGAAGG TGTGGTCCGG GGTCCCCGCG
    GAGATGCCGT GTGCCAGGAA ACAGCACTTT GAGCTGCTGG CTGAGCTGGA GCACGACGTC
    AGCGTGGATA CGATCAGTTT GAACGCTGCC GGCACCCTGC TGGCCTCAGG CACCAAGGAA
    GGCACAGTGA CCATCTGGGA CCTCACCACG GCCACCCTGT TACACCAGAT TCCATGTCAT
    GAGGGGACTG TAGGCGACAC TGCTTTCAGC CCAGACAGTC GCCACATCCT GAGCACAGGA
    GCCAACAGCC ATCTGAACGT TATCGACGTG CAGACAGGAA TGCTCATCTC TTCTCTGACT
    GCAGACGAGC CCCAGAGGTG CTTTCTCTGG GATGGAAACT CCGTCGTATC TGGGAGTCAG
    TCTGGGGAGC TGCTCATCTG GGACCTCCTT GGGGGAAGGG TCAGCAAGAG GATCCAGGGC
    CACTCAGGTA AGGTGACCTG CCTCTTCTCC CTACATGCTC CAGCGCCTCC TTTCCCACCA
    GTGGACACGC TCTTCTCAGG TTGCAAGCCC CCAGGGGCCT CTGTGCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025121157.1
    CDS1497..4007
    Translation

    Target ORF information:

    RefSeq Version XM_025265372.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025265372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGAAAC CCGTGGTCCA GATAATGCTC CAGGACATCC GCCGCATTTA CAAGAGGAGA
    CACGGCCTCA TGCCTCTGGG CTTGGAAGTG TTTTGCACAG AAGATGACCT GTGTTCCGAC
    ATCTACCTGA AGTTCTATGA ACGTCAGGAC CGAGACGACC TCTACTTTTA CATCGCTACG
    TACCTAGAGC ACCACGTGCC GGAGCGCACG GCCGAGGGCT ACACGCTGCA GTGGCAGCGC
    GGCCTCCTGT CCAACTACCA GTACCTGCTG CACCTCAACT CGCTGGCCGA CCGCAGCTGC
    AGCGACCTCT CCCAGTACCC CGTGTTCCCC TGGGTCGTCG GCGACTACAG CAGCTCCGAG
    CTGGATCTGT CAAATCCAGA GACCTTCCGG GATCTCAGTA AGCCAGTAGG GGCCCTCAAC
    CAGGAGCGTC TGGAGAGGCT GCTGACCCGC TACCAGGAGA TGCCGGAGCC CAGGTTCATG
    TACGGCAGCC ACTACTCCTC CCCAGGCTAC GTGCTCTTCT ACCTGGTCCG AATCGCGCCA
    GAGTACATGC TGTGCCTGCA GAATGGAAGG TTCGACAACG CAGACAGAAT GTTCAACAGT
    ATTGCAGAAA CTTGGAAAAA CTGTTTGGAT GGTGCAACCG ATTTTAAAGA GCTGATCCCG
    GAATTCTACG GTGATGATGT CAGCTTTTTA GTCAACAGTC TGAAGTTGGA TTTGGGGAAG
    AGACAAGGAG GGCAACTGGT TAACGACGTA GAGCTCCCGC CTTGGGCACG GAGTCCCGAG
    GATTTTCTCC GGAAGAGCAG AGCCGCGCTG GAGAGTGAGT ATGTGTCCGA GCACCTGCAT
    GAGTGGATCG ACCTGGTGTT TGGCTACAAG CAGCAGGGCC GGGATGCCGT CGGAGCCCAT
    AATGTGTTTC ATCCGCTGAC CTATGAAGGA GGTGTGGACT TGAACAGCAT CGAGGATCCT
    GACGAGAAGG TCGCCATGCT CACCCAGATC TTAGAGTTCG GACAGACACC CAGACAGCTC
    TTTGTGACAC CCCATCCTCG AAGAATCACC CCAAAGTTTA AAAACGTGTC CCAGCCCTCC
    AGTTACAATG CCTCCACGGC AGATTCTCCA GTCTCTCTAG GCGAAGAGTC TTTTGAAGAC
    CTGACAGAAG AGAGCAGAAC CCTGGCCTGG AGTAACATCG CCCGGCTAGA GCCCCAGGAG
    CAGCACAGAA TCCACAAAGA GGCAGTCACT GGAATAGCCG TGTCCTGCAA TGGGTCGTCC
    ATCTTCACAA CCTCACAAGA TTCCACCTTG AAGATGTTTT CCAAAGAATC CAGGATGCTA
    CAGAGAAGCA TATCATTCTC AAATATGGCT TTATCATCCT GTTTGCTTTT ACCAGGAGAT
    GCCACTGTCA TAAGCTCTTC CTGGGATAAT AACGTCTATT TTTATTCCAT AGCATTTGGA
    AGATGCCAGG ACACATTAAT GGGACATGAC GATGCTGTTA GTAAGATCTG TTGGCATGAC
    AACAGGCTGT ATTCTGCATC GTGGGACTCT ACTGTGAAGG TGTGGTCCGG GGTCCCCGCG
    GAGATGCCGT GTGCCAGGAA ACAGCACTTT GAGCTGCTGG CTGAGCTGGA GCACGACGTC
    AGCGTGGATA CGATCAGTTT GAACGCTGCC GGCACCCTGC TGGCCTCAGG CACCAAGGAA
    GGCACAGTGA CCATCTGGGA CCTCACCACG GCCACCCTGT TACACCAGAT TCCATGTCAT
    GAGGGGACTG TAGGCGACAC TGCTTTCAGC CCAGACAGTC GCCACATCCT GAGCACAGGA
    GCCAACAGCC ATCTGAACGT TATCGACGTG CAGACAGGAA TGCTCATCTC TTCTCTGACT
    GCAGACGAGC CCCAGAGGTG CTTTCTCTGG GATGGAAACT CCGTCGTATC TGGGAGTCAG
    TCTGGGGAGC TGCTCATCTG GGACCTCCTT GGGGGAAGGG TCAGCAAGAG GATCCAGGGC
    CACTCAGGTA AGGTGACCTG CCTCTTCTCC CTACATGCTC CAGCGCCTCC TTTCCCACCA
    GTGGACACGC TCTTCTCAGG TTGCAAGCCC CCAGGGGCCT CTGTGCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49508
    Clone ID Related Accession (Same CDS sequence) XM_025265373.1
    Accession Version XM_025265373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein FAN isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGAAAC CCGTGGTCCA GATAATGCTC CAGGACATCC GCCGCATTTA CAAGAGGAGA
    CACGGCCTCA TGCCTCTGGG CTTGGAAGTG TTTTGCACAG AAGATGACCT GTGTTCCGAC
    ATCTACCTGA AGTTCTATGA ACGTCAGGAC CGAGACGACC TCTACTTTTA CATCGCTACG
    TACCTAGAGC ACCACGTGCC GGAGCGCACG GCCGAGGGCT ACACGCTGCA GTGGCAGCGC
    GGCCTCCTGT CCAACTACCA GTACCTGCTG CACCTCAACT CGCTGGCCGA CCGCAGCTGC
    AGCGACCTCT CCCAGTACCC CGTGTTCCCC TGGGTCGTCG GCGACTACAG CAGCTCCGAG
    CTGGATCTGT CAAATCCAGA GACCTTCCGG GATCTCAGTA AGCCAGTAGG GGCCCTCAAC
    CAGGAGCGTC TGGAGAGGCT GCTGACCCGC TACCAGGAGA TGCCGGAGCC CAGGTTCATG
    TACGGCAGCC ACTACTCCTC CCCAGGCTAC GTGCTCTTCT ACCTGGTCCG AATCGCGCCA
    GAGTACATGC TGTGCCTGCA GAATGGAAGG TTCGACAACG CAGACAGAAT GTTCAACAGT
    ATTGCAGAAA CTTGGAAAAA CTGTTTGGAT GGTGCAACCG ATTTTAAAGA GCTGATCCCG
    GAATTCTACG GTGATGATGT CAGCTTTTTA GTCAACAGTC TGAAGTTGGA TTTGGGGAAG
    AGACAAGGAG GGCAACTGGT TAACGACGTA GAGCTCCCGC CTTGGGCACG GAGTCCCGAG
    GATTTTCTCC GGAAGAGCAG AGCCGCGCTG GAGAGTGAGT ATGTGTCCGA GCACCTGCAT
    GAGTGGATCG ACCTGGTGTT TGGCTACAAG CAGCAGGGCC GGGATGCCGT CGGAGCCCAT
    AATGTGTTTC ATCCGCTGAC CTATGAAGGA GGTGTGGACT TGAACAGCAT CGAGGATCCT
    GACGAGAAGG TCGCCATGCT CACCCAGATC TTAGAGTTCG GACAGACACC CAGACAGCTC
    TTTGTGACAC CCCATCCTCG AAGAATCACC CCAAAGTTTA AAAACGTGTC CCAGCCCTCC
    AGTTACAATG CCTCCACGGC AGATTCTCCA GTCTCTCTAG GCGAAGAGTC TTTTGAAGAC
    CTGACAGAAG AGAGCAGAAC CCTGGCCTGG AGTAACATCG CCCGGCTAGA GCCCCAGGAG
    CAGCACAGAA TCCACAAAGA GGCAGTCACT GGAATAGCCG TGTCCTGCAA TGGGTCGTCC
    ATCTTCACAA CCTCACAAGA TTCCACCTTG AAGATGTTTT CCAAAGAATC CAGGATGCTA
    CAGAGAAGCA TATCATTCTC AAATATGGCT TTATCATCCT GTTTGCTTTT ACCAGGAGAT
    GCCACTGTCA TAAGCTCTTC CTGGGATAAT AACGTCTATT TTTATTCCAT AGCATTTGGA
    AGATGCCAGG ACACATTAAT GGGACATGAC GATGCTGTTA GTAAGATCTG TTGGCATGAC
    AACAGGCTGT ATTCTGCATC GTGGGACTCT ACTGTGAAGG TGTGGTCCGG GGTCCCCGCG
    GAGATGCCGT GTGCCAGGAA ACAGCACTTT GAGCTGCTGG CTGAGCTGGA GCACGACGTC
    AGCGTGGATA CGATCAGTTT GAACGCTGCC GGCACCCTGC TGGCCTCAGG CACCAAGGAA
    GGCACAGTGA CCATCTGGGA CCTCACCACG GCCACCCTGT TACACCAGAT TCCATGTCAT
    GAGGGGACTG TAGGCGACAC TGCTTTCAGC CCAGACAGTC GCCACATCCT GAGCACAGGA
    GCCAACAGCC ATCTGAACGT TATCGACGTG CAGACAGGAA TGCTCATCTC TTCTCTGACT
    GCAGACGAGC CCCAGAGGTG CTTTCTCTGG GATGGAAACT CCGTCGTATC TGGGAGTCAG
    TCTGGGGAGC TGCTCATCTG GGACCTCCTT GGGGGAAGGG TCAGCAAGAG GATCCAGGGC
    CACTCAGGCG CCGTGACGTG CATGTGGATG AGCGAGCAGT GTAGCAGCGT CATCACAGGA
    GGGGAAGACA GACAAATTAT GTTCTGGAAG TCGCAGTATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025121158.1
    CDS1496..3997
    Translation

    Target ORF information:

    RefSeq Version XM_025265373.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025265373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGAAAC CCGTGGTCCA GATAATGCTC CAGGACATCC GCCGCATTTA CAAGAGGAGA
    CACGGCCTCA TGCCTCTGGG CTTGGAAGTG TTTTGCACAG AAGATGACCT GTGTTCCGAC
    ATCTACCTGA AGTTCTATGA ACGTCAGGAC CGAGACGACC TCTACTTTTA CATCGCTACG
    TACCTAGAGC ACCACGTGCC GGAGCGCACG GCCGAGGGCT ACACGCTGCA GTGGCAGCGC
    GGCCTCCTGT CCAACTACCA GTACCTGCTG CACCTCAACT CGCTGGCCGA CCGCAGCTGC
    AGCGACCTCT CCCAGTACCC CGTGTTCCCC TGGGTCGTCG GCGACTACAG CAGCTCCGAG
    CTGGATCTGT CAAATCCAGA GACCTTCCGG GATCTCAGTA AGCCAGTAGG GGCCCTCAAC
    CAGGAGCGTC TGGAGAGGCT GCTGACCCGC TACCAGGAGA TGCCGGAGCC CAGGTTCATG
    TACGGCAGCC ACTACTCCTC CCCAGGCTAC GTGCTCTTCT ACCTGGTCCG AATCGCGCCA
    GAGTACATGC TGTGCCTGCA GAATGGAAGG TTCGACAACG CAGACAGAAT GTTCAACAGT
    ATTGCAGAAA CTTGGAAAAA CTGTTTGGAT GGTGCAACCG ATTTTAAAGA GCTGATCCCG
    GAATTCTACG GTGATGATGT CAGCTTTTTA GTCAACAGTC TGAAGTTGGA TTTGGGGAAG
    AGACAAGGAG GGCAACTGGT TAACGACGTA GAGCTCCCGC CTTGGGCACG GAGTCCCGAG
    GATTTTCTCC GGAAGAGCAG AGCCGCGCTG GAGAGTGAGT ATGTGTCCGA GCACCTGCAT
    GAGTGGATCG ACCTGGTGTT TGGCTACAAG CAGCAGGGCC GGGATGCCGT CGGAGCCCAT
    AATGTGTTTC ATCCGCTGAC CTATGAAGGA GGTGTGGACT TGAACAGCAT CGAGGATCCT
    GACGAGAAGG TCGCCATGCT CACCCAGATC TTAGAGTTCG GACAGACACC CAGACAGCTC
    TTTGTGACAC CCCATCCTCG AAGAATCACC CCAAAGTTTA AAAACGTGTC CCAGCCCTCC
    AGTTACAATG CCTCCACGGC AGATTCTCCA GTCTCTCTAG GCGAAGAGTC TTTTGAAGAC
    CTGACAGAAG AGAGCAGAAC CCTGGCCTGG AGTAACATCG CCCGGCTAGA GCCCCAGGAG
    CAGCACAGAA TCCACAAAGA GGCAGTCACT GGAATAGCCG TGTCCTGCAA TGGGTCGTCC
    ATCTTCACAA CCTCACAAGA TTCCACCTTG AAGATGTTTT CCAAAGAATC CAGGATGCTA
    CAGAGAAGCA TATCATTCTC AAATATGGCT TTATCATCCT GTTTGCTTTT ACCAGGAGAT
    GCCACTGTCA TAAGCTCTTC CTGGGATAAT AACGTCTATT TTTATTCCAT AGCATTTGGA
    AGATGCCAGG ACACATTAAT GGGACATGAC GATGCTGTTA GTAAGATCTG TTGGCATGAC
    AACAGGCTGT ATTCTGCATC GTGGGACTCT ACTGTGAAGG TGTGGTCCGG GGTCCCCGCG
    GAGATGCCGT GTGCCAGGAA ACAGCACTTT GAGCTGCTGG CTGAGCTGGA GCACGACGTC
    AGCGTGGATA CGATCAGTTT GAACGCTGCC GGCACCCTGC TGGCCTCAGG CACCAAGGAA
    GGCACAGTGA CCATCTGGGA CCTCACCACG GCCACCCTGT TACACCAGAT TCCATGTCAT
    GAGGGGACTG TAGGCGACAC TGCTTTCAGC CCAGACAGTC GCCACATCCT GAGCACAGGA
    GCCAACAGCC ATCTGAACGT TATCGACGTG CAGACAGGAA TGCTCATCTC TTCTCTGACT
    GCAGACGAGC CCCAGAGGTG CTTTCTCTGG GATGGAAACT CCGTCGTATC TGGGAGTCAG
    TCTGGGGAGC TGCTCATCTG GGACCTCCTT GGGGGAAGGG TCAGCAAGAG GATCCAGGGC
    CACTCAGGCG CCGTGACGTG CATGTGGATG AGCGAGCAGT GTAGCAGCGT CATCACAGGA
    GGGGAAGACA GACAAATTAT GTTCTGGAAG TCGCAGTATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49509
    Clone ID Related Accession (Same CDS sequence) XM_025265374.1
    Accession Version XM_025265374.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2481bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein FAN isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGGTAG GCGGACTGCG GGCCAGCGAG AGCTCCTTTG CCCTGCAGTT TTCGTCTTCT
    AAACCCGTGG TCCAGATAAT GCTCCAGGAC ATCCGCCGCA TTTACAAGAG GAGACACGGC
    CTCATGCCTC TGGGCTTGGA AGTGTTTTGC ACAGAAGATG ACCTGTGTTC CGACATCTAC
    CTGAAGTTCT ATGAACGTCA GGACCGAGAC GACCTCTACT TTTACATCGC TACGTACCTA
    GAGCACCACG TGCCGGAGCG CACGGCCGAG GGCTACACGC TGCAGTGGCA GCGCGGCCTC
    CTGTCCAACT ACCAGTACCT GCTGCACCTC AACTCGCTGG CCGACCGCAG CTGCAGCGAC
    CTCTCCCAGT ACCCCGTGTT CCCCTGGGTC GTCGGCGACT ACAGCAGCTC CGAGCTGGAT
    CTGTCAAATC CAGAGACCTT CCGGGATCTC AGTAAGCCAG TAGGGGCCCT CAACCAGGAG
    CGTCTGGAGA GGCTGCTGAC CCGCTACCAG GAGATGCCGG AGCCCAGGTT CATGTACGGC
    AGCCACTACT CCTCCCCAGG CTACGTGCTC TTCTACCTGG TCCGAATCGC GCCAGAGTAC
    ATGCTGTGCC TGCAGAATGG AAGGTTCGAC AACGCAGACA GAATGTTCAA CAGTATTGCA
    GAAACTTGGA AAAACTGTTT GGATGGTGCA ACCGATTTTA AAGAGCTGAT CCCGGAATTC
    TACGGTGATG ATGTCAGCTT TTTAGTCAAC AGTCTGAAGT TGGATTTGGG GAAGAGACAA
    GGAGGGCAAC TGGTTAACGA CGTAGAGCTC CCGCCTTGGG CACGGAGTCC CGAGGATTTT
    CTCCGGAAGA GCAGAGCCGC GCTGGAGAGT GAGTATGTGT CCGAGCACCT GCATGAGTGG
    ATCGACCTGG TGTTTGGCTA CAAGCAGCAG GGCCGGGATG CCGTCGGAGC CCATAATGTG
    TTTCATCCGC TGACCTATGA AGGAGGTGTG GACTTGAACA GCATCGAGGA TCCTGACGAG
    AAGGTCGCCA TGCTCACCCA GATCTTAGAG TTCGGACAGA CACCCAGACA GCTCTTTGTG
    ACACCCCATC CTCGAAGAAT CACCCCAAAG TTTAAAAACG TGTCCCAGCC CTCCAGTTAC
    AATGCCTCCA CGGCAGATTC TCCAGTCTCT CTAGGCGAAG AGTCTTTTGA AGACCTGACA
    GAAGAGAGCA GAACCCTGGC CTGGAGTAAC ATCGCCCGGC TAGAGCCCCA GGAGCAGCAC
    AGAATCCACA AAGAGGCAGT CACTGGAATA GCCGTGTCCT GCAATGGGTC GTCCATCTTC
    ACAACCTCAC AAGATTCCAC CTTGAAGATG TTTTCCAAAG AATCCAGGAT GCTACAGAGA
    AGCATATCAT TCTCAAATAT GGCTTTATCA TCCTGTTTGC TTTTACCAGG AGATGCCACT
    GTCATAAGCT CTTCCTGGGA TAATAACGTC TATTTTTATT CCATAGCATT TGGAAGATGC
    CAGGACACAT TAATGGGACA TGACGATGCT GTTAGTAAGA TCTGTTGGCA TGACAACAGG
    CTGTATTCTG CATCGTGGGA CTCTACTGTG AAGGTGTGGT CCGGGGTCCC CGCGGAGATG
    CCGTGTGCCA GGAAACAGCA CTTTGAGCTG CTGGCTGAGC TGGAGCACGA CGTCAGCGTG
    GATACGATCA GTTTGAACGC TGCCGGCACC CTGCTGGCCT CAGGCACCAA GGAAGGCACA
    GTGACCATCT GGGACCTCAC CACGGCCACC CTGTTACACC AGATTCCATG TCATGAGGGG
    ACTGTAGGCG ACACTGCTTT CAGCCCAGAC AGTCGCCACA TCCTGAGCAC AGGAGCCAAC
    AGCCATCTGA ACGTTATCGA CGTGCAGACA GGAATGCTCA TCTCTTCTCT GACTGCAGAC
    GAGCCCCAGA GGTGCTTTCT CTGGGATGGA AACTCCGTCG TATCTGGGAG TCAGTCTGGG
    GAGCTGCTCA TCTGGGACCT CCTTGGGGGA AGGGTCAGCA AGAGGATCCA GGGCCACTCA
    GAGCCAAGTC CAGACACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025121159.1
    CDS1499..3979
    Translation

    Target ORF information:

    RefSeq Version XM_025265374.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025265374.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGGTAG GCGGACTGCG GGCCAGCGAG AGCTCCTTTG CCCTGCAGTT TTCGTCTTCT
    AAACCCGTGG TCCAGATAAT GCTCCAGGAC ATCCGCCGCA TTTACAAGAG GAGACACGGC
    CTCATGCCTC TGGGCTTGGA AGTGTTTTGC ACAGAAGATG ACCTGTGTTC CGACATCTAC
    CTGAAGTTCT ATGAACGTCA GGACCGAGAC GACCTCTACT TTTACATCGC TACGTACCTA
    GAGCACCACG TGCCGGAGCG CACGGCCGAG GGCTACACGC TGCAGTGGCA GCGCGGCCTC
    CTGTCCAACT ACCAGTACCT GCTGCACCTC AACTCGCTGG CCGACCGCAG CTGCAGCGAC
    CTCTCCCAGT ACCCCGTGTT CCCCTGGGTC GTCGGCGACT ACAGCAGCTC CGAGCTGGAT
    CTGTCAAATC CAGAGACCTT CCGGGATCTC AGTAAGCCAG TAGGGGCCCT CAACCAGGAG
    CGTCTGGAGA GGCTGCTGAC CCGCTACCAG GAGATGCCGG AGCCCAGGTT CATGTACGGC
    AGCCACTACT CCTCCCCAGG CTACGTGCTC TTCTACCTGG TCCGAATCGC GCCAGAGTAC
    ATGCTGTGCC TGCAGAATGG AAGGTTCGAC AACGCAGACA GAATGTTCAA CAGTATTGCA
    GAAACTTGGA AAAACTGTTT GGATGGTGCA ACCGATTTTA AAGAGCTGAT CCCGGAATTC
    TACGGTGATG ATGTCAGCTT TTTAGTCAAC AGTCTGAAGT TGGATTTGGG GAAGAGACAA
    GGAGGGCAAC TGGTTAACGA CGTAGAGCTC CCGCCTTGGG CACGGAGTCC CGAGGATTTT
    CTCCGGAAGA GCAGAGCCGC GCTGGAGAGT GAGTATGTGT CCGAGCACCT GCATGAGTGG
    ATCGACCTGG TGTTTGGCTA CAAGCAGCAG GGCCGGGATG CCGTCGGAGC CCATAATGTG
    TTTCATCCGC TGACCTATGA AGGAGGTGTG GACTTGAACA GCATCGAGGA TCCTGACGAG
    AAGGTCGCCA TGCTCACCCA GATCTTAGAG TTCGGACAGA CACCCAGACA GCTCTTTGTG
    ACACCCCATC CTCGAAGAAT CACCCCAAAG TTTAAAAACG TGTCCCAGCC CTCCAGTTAC
    AATGCCTCCA CGGCAGATTC TCCAGTCTCT CTAGGCGAAG AGTCTTTTGA AGACCTGACA
    GAAGAGAGCA GAACCCTGGC CTGGAGTAAC ATCGCCCGGC TAGAGCCCCA GGAGCAGCAC
    AGAATCCACA AAGAGGCAGT CACTGGAATA GCCGTGTCCT GCAATGGGTC GTCCATCTTC
    ACAACCTCAC AAGATTCCAC CTTGAAGATG TTTTCCAAAG AATCCAGGAT GCTACAGAGA
    AGCATATCAT TCTCAAATAT GGCTTTATCA TCCTGTTTGC TTTTACCAGG AGATGCCACT
    GTCATAAGCT CTTCCTGGGA TAATAACGTC TATTTTTATT CCATAGCATT TGGAAGATGC
    CAGGACACAT TAATGGGACA TGACGATGCT GTTAGTAAGA TCTGTTGGCA TGACAACAGG
    CTGTATTCTG CATCGTGGGA CTCTACTGTG AAGGTGTGGT CCGGGGTCCC CGCGGAGATG
    CCGTGTGCCA GGAAACAGCA CTTTGAGCTG CTGGCTGAGC TGGAGCACGA CGTCAGCGTG
    GATACGATCA GTTTGAACGC TGCCGGCACC CTGCTGGCCT CAGGCACCAA GGAAGGCACA
    GTGACCATCT GGGACCTCAC CACGGCCACC CTGTTACACC AGATTCCATG TCATGAGGGG
    ACTGTAGGCG ACACTGCTTT CAGCCCAGAC AGTCGCCACA TCCTGAGCAC AGGAGCCAAC
    AGCCATCTGA ACGTTATCGA CGTGCAGACA GGAATGCTCA TCTCTTCTCT GACTGCAGAC
    GAGCCCCAGA GGTGCTTTCT CTGGGATGGA AACTCCGTCG TATCTGGGAG TCAGTCTGGG
    GAGCTGCTCA TCTGGGACCT CCTTGGGGGA AGGGTCAGCA AGAGGATCCA GGGCCACTCA
    GAGCCAAGTC CAGACACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49510
    Clone ID Related Accession (Same CDS sequence) XM_025265375.1
    Accession Version XM_025265375.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2475bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein FAN isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGGTAG GCGGACTGCG GGCCAGCGAG AGCTCCTTTG CCCTGCAGTT TTCGTCTTCT
    AAACCCGTGG TCCAGATAAT GCTCCAGGAC ATCCGCCGCA TTTACAAGAG GAGACACGGC
    CTCATGCCTC TGGGCTTGGA AGTGTTTTGC ACAGAAGATG ACCTGTGTTC CGACATCTAC
    CTGAAGTTCT ATGAACGTCA GGACCGAGAC GACCTCTACT TTTACATCGC TACGTACCTA
    GAGCACCACG TGCCGGAGCG CACGGCCGAG GGCTACACGC TGCAGTGGCA GCGCGGCCTC
    CTGTCCAACT ACCAGTACCT GCTGCACCTC AACTCGCTGG CCGACCGCAG CTGCAGCGAC
    CTCTCCCAGT ACCCCGTGTT CCCCTGGGTC GTCGGCGACT ACAGCAGCTC CGAGCTGGAT
    CTGTCAAATC CAGAGACCTT CCGGGATCTC AGTAAGCCAG TAGGGGCCCT CAACCAGGAG
    CGTCTGGAGA GGCTGCTGAC CCGCTACCAG GAGATGCCGG AGCCCAGGTT CATGTACGGC
    AGCCACTACT CCTCCCCAGG CTACGTGCTC TTCTACCTGG TCCGAATCGC GCCAGAGTAC
    ATGCTGTGCC TGCAGAATGG AAGGTTCGAC AACGCAGACA GAATGTTCAA CAGTATTGCA
    GAAACTTGGA AAAACTGTTT GGATGGTGCA ACCGATTTTA AAGAGCTGAT CCCGGAATTC
    TACGGTGATG ATGTCAGCTT TTTAGTCAAC AGTCTGAAGT TGGATTTGGG GAAGAGACAA
    GGAGGGCAAC TGGTTAACGA CGTAGAGCTC CCGCCTTGGG CACGGAGTCC CGAGGATTTT
    CTCCGGAAGA GCAGAGCCGC GCTGGAGAGT GAGTATGTGT CCGAGCACCT GCATGAGTGG
    ATCGACCTGG TGTTTGGCTA CAAGCAGCAG GGCCGGGATG CCGTCGGAGC CCATAATGTG
    TTTCATCCGC TGACCTATGA AGGAGGTGTG GACTTGAACA GCATCGAGGA TCCTGACGAG
    AAGGTCGCCA TGCTCACCCA GATCTTAGAG TTCGGACAGA CACCCAGACA GCTCTTTGTG
    ACACCCCATC CTCGAAGAAT CACCCCAAAG TTTAAAAACG TGTCCCAGCC CTCCAGTTAC
    AATGCCTCCA CGGCAGATTC TCCAGTCTCT CTAGGCGAAG AGTCTTTTGA AGACCTGACA
    GAAGAGAGCA GAACCCTGGC CTGGAGTAAC ATCGCCCGGC TAGAGCCCCA GGAGCAGCAC
    AGAATCCACA AAGAGGCAGT CACTGGAATA GCCGTGTCCT GCAATGGGTC GTCCATCTTC
    ACAACCTCAC AAGATTCCAC CTTGAAGATG TTTTCCAAAG AATCCAGGAT GCTACAGAGA
    AGCATATCAT TCTCAAATAT GGCTTTATCA TCCTGTTTGC TTTTACCAGG AGATGCCACT
    GTCATAAGCT CTTCCTGGGA TAATAACGTC TATTTTTATT CCATAGCATT TGGAAGATGC
    CAGGACACAT TAATGGGACA TGACGATGCT GTTAGTAAGA TCTGTTGGCA TGACAACAGG
    CTGTATTCTG CATCGTGGGA CTCTACTGTG AAGGTGTGGT CCGGGGTCCC CGCGGAGATG
    CCGTGTGCCA GGAAACAGCA CTTTGAGCTG CTGGCTGAGC TGGAGCACGA CGTCAGCGTG
    GATACGATCA GTTTGAACGC TGCCGGCACC CTGCTGGCCT CAGGCACCAA GGAAGGCACA
    GTGACCATCT GGGACCTCAC CACGGCCACC CTGTTACACC AGATTCCATG TCATGAGGGG
    ACTGTAGGCG ACACTGCTTT CAGCCCAGAC AGTCGCCACA TCCTGAGCAC AGGAGCCAAC
    AGCCATCTGA ACGTTATCGA CGTGCAGACA GGAATGCTCA TCTCTTCTCT GACTGCAGAC
    GAGCCCCAGA GGTGCTTTCT CTGGGATGGA AACTCCGTCG TATCTGGGAG TCAGTCTGGG
    GAGCTGCTCA TCTGGGACCT CCTTGGGGGA AGGGTCAGCA AGAGGATCCA GGGCCACTCA
    GAAAACCATC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025121160.1
    CDS1499..3973
    Translation

    Target ORF information:

    RefSeq Version XM_025265375.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025265375.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGAAAA TGAAGCCAGT AGACACTTTG CAAAGACAGT ATCTGGGGCG AATTTCACTC 
    ATTTTCAGCC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA
    AGGGGCACGA TGGAGTATGT CTTTGAGCTG GACGTTTCAG GGAAAGTGGA AGATGTTGTG
    GAGACACTCC TTCAACTTCA TAGAGCATCC TGTCTGGACA AACTGGGGGA CCAAACTGCC
    ATGATAACAG CTATCTTGCA GTCCCGTTTG GCTAGGACGT CATTCGACAA AAACAGGTTC
    CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCTCTGGTG
    ACGAACCCTG GCCACGCCTG CGTCACTGAC AGCACCCTCT ATTTCCAGCC TCTCAACGGG
    TACCCGGTAG GCGGACTGCG GGCCAGCGAG AGCTCCTTTG CCCTGCAGTT TTCGTCTTCT
    AAACCCGTGG TCCAGATAAT GCTCCAGGAC ATCCGCCGCA TTTACAAGAG GAGACACGGC
    CTCATGCCTC TGGGCTTGGA AGTGTTTTGC ACAGAAGATG ACCTGTGTTC CGACATCTAC
    CTGAAGTTCT ATGAACGTCA GGACCGAGAC GACCTCTACT TTTACATCGC TACGTACCTA
    GAGCACCACG TGCCGGAGCG CACGGCCGAG GGCTACACGC TGCAGTGGCA GCGCGGCCTC
    CTGTCCAACT ACCAGTACCT GCTGCACCTC AACTCGCTGG CCGACCGCAG CTGCAGCGAC
    CTCTCCCAGT ACCCCGTGTT CCCCTGGGTC GTCGGCGACT ACAGCAGCTC CGAGCTGGAT
    CTGTCAAATC CAGAGACCTT CCGGGATCTC AGTAAGCCAG TAGGGGCCCT CAACCAGGAG
    CGTCTGGAGA GGCTGCTGAC CCGCTACCAG GAGATGCCGG AGCCCAGGTT CATGTACGGC
    AGCCACTACT CCTCCCCAGG CTACGTGCTC TTCTACCTGG TCCGAATCGC GCCAGAGTAC
    ATGCTGTGCC TGCAGAATGG AAGGTTCGAC AACGCAGACA GAATGTTCAA CAGTATTGCA
    GAAACTTGGA AAAACTGTTT GGATGGTGCA ACCGATTTTA AAGAGCTGAT CCCGGAATTC
    TACGGTGATG ATGTCAGCTT TTTAGTCAAC AGTCTGAAGT TGGATTTGGG GAAGAGACAA
    GGAGGGCAAC TGGTTAACGA CGTAGAGCTC CCGCCTTGGG CACGGAGTCC CGAGGATTTT
    CTCCGGAAGA GCAGAGCCGC GCTGGAGAGT GAGTATGTGT CCGAGCACCT GCATGAGTGG
    ATCGACCTGG TGTTTGGCTA CAAGCAGCAG GGCCGGGATG CCGTCGGAGC CCATAATGTG
    TTTCATCCGC TGACCTATGA AGGAGGTGTG GACTTGAACA GCATCGAGGA TCCTGACGAG
    AAGGTCGCCA TGCTCACCCA GATCTTAGAG TTCGGACAGA CACCCAGACA GCTCTTTGTG
    ACACCCCATC CTCGAAGAAT CACCCCAAAG TTTAAAAACG TGTCCCAGCC CTCCAGTTAC
    AATGCCTCCA CGGCAGATTC TCCAGTCTCT CTAGGCGAAG AGTCTTTTGA AGACCTGACA
    GAAGAGAGCA GAACCCTGGC CTGGAGTAAC ATCGCCCGGC TAGAGCCCCA GGAGCAGCAC
    AGAATCCACA AAGAGGCAGT CACTGGAATA GCCGTGTCCT GCAATGGGTC GTCCATCTTC
    ACAACCTCAC AAGATTCCAC CTTGAAGATG TTTTCCAAAG AATCCAGGAT GCTACAGAGA
    AGCATATCAT TCTCAAATAT GGCTTTATCA TCCTGTTTGC TTTTACCAGG AGATGCCACT
    GTCATAAGCT CTTCCTGGGA TAATAACGTC TATTTTTATT CCATAGCATT TGGAAGATGC
    CAGGACACAT TAATGGGACA TGACGATGCT GTTAGTAAGA TCTGTTGGCA TGACAACAGG
    CTGTATTCTG CATCGTGGGA CTCTACTGTG AAGGTGTGGT CCGGGGTCCC CGCGGAGATG
    CCGTGTGCCA GGAAACAGCA CTTTGAGCTG CTGGCTGAGC TGGAGCACGA CGTCAGCGTG
    GATACGATCA GTTTGAACGC TGCCGGCACC CTGCTGGCCT CAGGCACCAA GGAAGGCACA
    GTGACCATCT GGGACCTCAC CACGGCCACC CTGTTACACC AGATTCCATG TCATGAGGGG
    ACTGTAGGCG ACACTGCTTT CAGCCCAGAC AGTCGCCACA TCCTGAGCAC AGGAGCCAAC
    AGCCATCTGA ACGTTATCGA CGTGCAGACA GGAATGCTCA TCTCTTCTCT GACTGCAGAC
    GAGCCCCAGA GGTGCTTTCT CTGGGATGGA AACTCCGTCG TATCTGGGAG TCAGTCTGGG
    GAGCTGCTCA TCTGGGACCT CCTTGGGGGA AGGGTCAGCA AGAGGATCCA GGGCCACTCA
    GAAAACCATC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu49511
    Clone ID Related Accession (Same CDS sequence) XM_025265376.1
    Accession Version XM_025265376.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2763bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product protein FAN isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAGGGAGAGA 
    TTTTCCTTGC TTCTGCTTAA CTTGGAGGAG TACTACTTTG AACAGCACAC GGCTCATCAC
    ATTCAGCACA AGGGCAGCCA GAAGGAAAGG AAAATCAGAG GCTCCTTAAA AATATGTTCA
    AAGTCAGTTA TTTTTGAACC GGATGCAATA TCCCAACCCA TCATTAAGAT TCCTTTGAGG
    GATTGTATAA AAATAGGAAG ACATGGAGAA AATGAAGCCA GTAGACACTT TGCAAAGTAT
    TATCTGGGGC GAATTTCACT CATTTTCAGC CAGGTGTATT TCATTAAAGA ACACAACATT
    GTTGCACCAT ATAAAATAGA AAGGGGCACG ATGGAGTATG TCTTTGAGCT GGACGTTTCA
    GGGAAAGTGG AAGATGTTGT GGAGACACTC CTTCAACTTC ATAGAGCATC CTGTCTGGAC
    AAACTGGGGG ACCAAACTGC CATGATAACA GCTATCTTGC AGTCCCGTTT GGCTAGGACG
    TCATTCGACA AAAACAGGTT CCAAAGTGTT TCTGAAAAGC TGCACATGGA ATGCAAAGCA
    GAAATGGTGA CACCTCTGGT GACGAACCCT GGCCACGCCT GCGTCACTGA CAGCACCCTC
    TATTTCCAGC CTCTCAACGG GTACCCGAAA CCCGTGGTCC AGATAATGCT CCAGGACATC
    CGCCGCATTT ACAAGAGGAG ACACGGCCTC ATGCCTCTGG GCTTGGAAGT GTTTTGCACA
    GAAGATGACC TGTGTTCCGA CATCTACCTG AAGTTCTATG AACGTCAGGA CCGAGACGAC
    CTCTACTTTT ACATCGCTAC GTACCTAGAG CACCACGTGC CGGAGCGCAC GGCCGAGGGC
    TACACGCTGC AGTGGCAGCG CGGCCTCCTG TCCAACTACC AGTACCTGCT GCACCTCAAC
    TCGCTGGCCG ACCGCAGCTG CAGCGACCTC TCCCAGTACC CCGTGTTCCC CTGGGTCGTC
    GGCGACTACA GCAGCTCCGA GCTGGATCTG TCAAATCCAG AGACCTTCCG GGATCTCAGT
    AAGCCAGTAG GGGCCCTCAA CCAGGAGCGT CTGGAGAGGC TGCTGACCCG CTACCAGGAG
    ATGCCGGAGC CCAGGTTCAT GTACGGCAGC CACTACTCCT CCCCAGGCTA CGTGCTCTTC
    TACCTGGTCC GAATCGCGCC AGAGTACATG CTGTGCCTGC AGAATGGAAG GTTCGACAAC
    GCAGACAGAA TGTTCAACAG TATTGCAGAA ACTTGGAAAA ACTGTTTGGA TGGTGCAACC
    GATTTTAAAG AGCTGATCCC GGAATTCTAC GGTGATGATG TCAGCTTTTT AGTCAACAGT
    CTGAAGTTGG ATTTGGGGAA GAGACAAGGA GGGCAACTGG TTAACGACGT AGAGCTCCCG
    CCTTGGGCAC GGAGTCCCGA GGATTTTCTC CGGAAGAGCA GAGCCGCGCT GGAGAGTGAG
    TATGTGTCCG AGCACCTGCA TGAGTGGATC GACCTGGTGT TTGGCTACAA GCAGCAGGGC
    CGGGATGCCG TCGGAGCCCA TAATGTGTTT CATCCGCTGA CCTATGAAGG AGGTGTGGAC
    TTGAACAGCA TCGAGGATCC TGACGAGAAG GTCGCCATGC TCACCCAGAT CTTAGAGTTC
    GGACAGACAC CCAGACAGCT CTTTGTGACA CCCCATCCTC GAAGAATCAC CCCAAAGTTT
    AAAAACGTGT CCCAGCCCTC CAGTTACAAT GCCTCCACGG CAGATTCTCC AGTCTCTCTA
    GGCGAAGAGT CTTTTGAAGA CCTGACAGAA GAGAGCAGAA CCCTGGCCTG GAGTAACATC
    GCCCGGCTAG AGCCCCAGGA GCAGCACAGA ATCCACAAAG AGGCAGTCAC TGGAATAGCC
    GTGTCCTGCA ATGGGTCGTC CATCTTCACA ACCTCACAAG ATTCCACCTT GAAGATGTTT
    TCCAAAGAAT CCAGGATGCT ACAGAGAAGC ATATCATTCT CAAATATGGC TTTATCATCC
    TGTTTGCTTT TACCAGGAGA TGCCACTGTC ATAAGCTCTT CCTGGGATAA TAACGTCTAT
    TTTTATTCCA TAGCATTTGG AAGATGCCAG GACACATTAA TGGGACATGA CGATGCTGTT
    AGTAAGATCT GTTGGCATGA CAACAGGCTG TATTCTGCAT CGTGGGACTC TACTGTGAAG
    GTGTGGTCCG GGGTCCCCGC GGAGATGCCG TGTGCCAGGA AACAGCACTT TGAGCTGCTG
    GCTGAGCTGG AGCACGACGT CAGCGTGGAT ACGATCAGTT TGAACGCTGC CGGCACCCTG
    CTGGCCTCAG GCACCAAGGA AGGCACAGTG ACCATCTGGG ACCTCACCAC GGCCACCCTG
    TTACACCAGA TTCCATGTCA TGAGGGGACT GTAGGCGACA CTGCTTTCAG CCCAGACAGT
    CGCCACATCC TGAGCACAGG AGCCAACAGC CATCTGAACG TTATCGACGT GCAGACAGGA
    ATGCTCATCT CTTCTCTGAC TGCAGACGAG CCCCAGAGGT GCTTTCTCTG GGATGGAAAC
    TCCGTCGTAT CTGGGAGTCA GTCTGGGGAG CTGCTCATCT GGGACCTCCT TGGGGGAAGG
    GTCAGCAAGA GGATCCAGGG CCACTCAGGC GCCGTGACGT GCATGTGGAT GAGCGAGCAG
    TGTAGCAGCG TCATCACAGG AGGGGAAGAC AGACAAATTA TGTTCTGGAA GTCGCAGTAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025121161.1
    CDS1237..3999
    Translation

    Target ORF information:

    RefSeq Version XM_025265376.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neutral sphingomyelinase activation associated factor (NSMAF), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025265376.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAGGGAGAGA 
    TTTTCCTTGC TTCTGCTTAA CTTGGAGGAG TACTACTTTG AACAGCACAC GGCTCATCAC
    ATTCAGCACA AGGGCAGCCA GAAGGAAAGG AAAATCAGAG GCTCCTTAAA AATATGTTCA
    AAGTCAGTTA TTTTTGAACC GGATGCAATA TCCCAACCCA TCATTAAGAT TCCTTTGAGG
    GATTGTATAA AAATAGGAAG ACATGGAGAA AATGAAGCCA GTAGACACTT TGCAAAGTAT
    TATCTGGGGC GAATTTCACT CATTTTCAGC CAGGTGTATT TCATTAAAGA ACACAACATT
    GTTGCACCAT ATAAAATAGA AAGGGGCACG ATGGAGTATG TCTTTGAGCT GGACGTTTCA
    GGGAAAGTGG AAGATGTTGT GGAGACACTC CTTCAACTTC ATAGAGCATC CTGTCTGGAC
    AAACTGGGGG ACCAAACTGC CATGATAACA GCTATCTTGC AGTCCCGTTT GGCTAGGACG
    TCATTCGACA AAAACAGGTT CCAAAGTGTT TCTGAAAAGC TGCACATGGA ATGCAAAGCA
    GAAATGGTGA CACCTCTGGT GACGAACCCT GGCCACGCCT GCGTCACTGA CAGCACCCTC
    TATTTCCAGC CTCTCAACGG GTACCCGAAA CCCGTGGTCC AGATAATGCT CCAGGACATC
    CGCCGCATTT ACAAGAGGAG ACACGGCCTC ATGCCTCTGG GCTTGGAAGT GTTTTGCACA
    GAAGATGACC TGTGTTCCGA CATCTACCTG AAGTTCTATG AACGTCAGGA CCGAGACGAC
    CTCTACTTTT ACATCGCTAC GTACCTAGAG CACCACGTGC CGGAGCGCAC GGCCGAGGGC
    TACACGCTGC AGTGGCAGCG CGGCCTCCTG TCCAACTACC AGTACCTGCT GCACCTCAAC
    TCGCTGGCCG ACCGCAGCTG CAGCGACCTC TCCCAGTACC CCGTGTTCCC CTGGGTCGTC
    GGCGACTACA GCAGCTCCGA GCTGGATCTG TCAAATCCAG AGACCTTCCG GGATCTCAGT
    AAGCCAGTAG GGGCCCTCAA CCAGGAGCGT CTGGAGAGGC TGCTGACCCG CTACCAGGAG
    ATGCCGGAGC CCAGGTTCAT GTACGGCAGC CACTACTCCT CCCCAGGCTA CGTGCTCTTC
    TACCTGGTCC GAATCGCGCC AGAGTACATG CTGTGCCTGC AGAATGGAAG GTTCGACAAC
    GCAGACAGAA TGTTCAACAG TATTGCAGAA ACTTGGAAAA ACTGTTTGGA TGGTGCAACC
    GATTTTAAAG AGCTGATCCC GGAATTCTAC GGTGATGATG TCAGCTTTTT AGTCAACAGT
    CTGAAGTTGG ATTTGGGGAA GAGACAAGGA GGGCAACTGG TTAACGACGT AGAGCTCCCG
    CCTTGGGCAC GGAGTCCCGA GGATTTTCTC CGGAAGAGCA GAGCCGCGCT GGAGAGTGAG
    TATGTGTCCG AGCACCTGCA TGAGTGGATC GACCTGGTGT TTGGCTACAA GCAGCAGGGC
    CGGGATGCCG TCGGAGCCCA TAATGTGTTT CATCCGCTGA CCTATGAAGG AGGTGTGGAC
    TTGAACAGCA TCGAGGATCC TGACGAGAAG GTCGCCATGC TCACCCAGAT CTTAGAGTTC
    GGACAGACAC CCAGACAGCT CTTTGTGACA CCCCATCCTC GAAGAATCAC CCCAAAGTTT
    AAAAACGTGT CCCAGCCCTC CAGTTACAAT GCCTCCACGG CAGATTCTCC AGTCTCTCTA
    GGCGAAGAGT CTTTTGAAGA CCTGACAGAA GAGAGCAGAA CCCTGGCCTG GAGTAACATC
    GCCCGGCTAG AGCCCCAGGA GCAGCACAGA ATCCACAAAG AGGCAGTCAC TGGAATAGCC
    GTGTCCTGCA ATGGGTCGTC CATCTTCACA ACCTCACAAG ATTCCACCTT GAAGATGTTT
    TCCAAAGAAT CCAGGATGCT ACAGAGAAGC ATATCATTCT CAAATATGGC TTTATCATCC
    TGTTTGCTTT TACCAGGAGA TGCCACTGTC ATAAGCTCTT CCTGGGATAA TAACGTCTAT
    TTTTATTCCA TAGCATTTGG AAGATGCCAG GACACATTAA TGGGACATGA CGATGCTGTT
    AGTAAGATCT GTTGGCATGA CAACAGGCTG TATTCTGCAT CGTGGGACTC TACTGTGAAG
    GTGTGGTCCG GGGTCCCCGC GGAGATGCCG TGTGCCAGGA AACAGCACTT TGAGCTGCTG
    GCTGAGCTGG AGCACGACGT CAGCGTGGAT ACGATCAGTT TGAACGCTGC CGGCACCCTG
    CTGGCCTCAG GCACCAAGGA AGGCACAGTG ACCATCTGGG ACCTCACCAC GGCCACCCTG
    TTACACCAGA TTCCATGTCA TGAGGGGACT GTAGGCGACA CTGCTTTCAG CCCAGACAGT
    CGCCACATCC TGAGCACAGG AGCCAACAGC CATCTGAACG TTATCGACGT GCAGACAGGA
    ATGCTCATCT CTTCTCTGAC TGCAGACGAG CCCCAGAGGT GCTTTCTCTG GGATGGAAAC
    TCCGTCGTAT CTGGGAGTCA GTCTGGGGAG CTGCTCATCT GGGACCTCCT TGGGGGAAGG
    GTCAGCAAGA GGATCCAGGG CCACTCAGGC GCCGTGACGT GCATGTGGAT GAGCGAGCAG
    TGTAGCAGCG TCATCACAGG AGGGGAAGAC AGACAAATTA TGTTCTGGAA GTCGCAGTAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu04644
    Clone ID Related Accession (Same CDS sequence) XM_006045655.1
    Accession Version XM_006045655.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product protein FAN
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783766.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGTCCT GTGGGCAGCC AGAAGTTAGC ATCGTCCCCC TGCTCCGGGG GCCTTATCCG 
    GGAAAAGCAG TGAAATGGAG CCGCGGGGCG GCGATTTGGG GCGGAGGGAC GGACCCCAGG
    CCGGGCCCTG GGTCCCGGGC GCCTCGGGCA AGCGCCCGCC CCTCCTCCGA CCTGAGTCAC
    GGCCGAGGCC CCACGCGGCG GGGATGCGGC GCTGAACGCG GGCTCGGGAC GCCAGGCCGC
    AGGCGCGCTC TCCTCGTGGG GCCCGCAGGC CCGCTGTGCC CGGCCGGCCT CCCGGGCTCC
    GGACCTCTCG GCCGGCGGCG GCGCCGCGGA ACCCGAGCTG GAGACGCGCG CCCCGCCGCC
    CTCCATGGCG TTCACCCGGA AGAGGCAGCG CGAGCAGCAG CTGCAGCTCT ACTCCAGGGA
    AGATTTTCCT TGCTTCTGCT TAACTTGGAG GAGTACTACT TTGAACAGCA CACGGCTCAT
    CACATTCAGC ACAAGGGCAG CCAGAAGGAA AGGAAAATCA GAGGCTCCTT AAAAATATGT
    TCAAAGTCAG TTATTTTTGA ACCGGATGCA ATATCCCAAC CCATCATTAA GATTCCTTTG
    AGGGATTGTA TAAAAATAGG AAGACATGGA GAAAATGAAG CCAGTAGACA CTTTGCAAAG
    ACAGTATCTG GGGCAATTTC ACTCATTTTC AGCCAGGTGT ATTTCATTAA AGAACACAAC
    ATTGTTGCAC CATATAAAAT AGAAAGGGGC ACGATGGAGT ATGTCTTTGA GCTGGACGTT
    TCAGGGAAAG TGGAAGATGT TGTGGAGACA CTCCTTCAAC TTCATAGAGC ATCCTGTCTG
    GACAAACTGG GGGACCAAAC TGCCATGATA ACAGCTATCT TGCAGTCCCG TTTGGCTAGG
    ACGTCATTCG ACAAAAACAG GTTCCAAAGT GTTTCTGAAA AGCTGCACAT GGAATGCAAA
    GCAGAAATGG TGACACCTCT GGTGACGAAC CCTGGCCACG CCTGCGTCAC TGACAGCACC
    CTCTATTTCC AGCCTCTCAA CGGGTACCCG AAACCCGTGG TCCAGATAAT GCTCCAGGAC
    ATCCGCCGCA TTTACAAGAG GAGACACGGC CTCATGCCTC TGGGCTTGGA AGTGTTTTGC
    ACAGAAGATG ACCTGTGTTC CGACATCTAC CTGAAGTTCT ATGAACGTCA GGACCGAGAC
    GACCTCTACT TTTACATCGC TACGTACCTA GAGCACCACG TGCCGGAGCG CACGGCCGAG
    GGCTACACGC TGCAGTGGCA GCGCGGCCTC CTGTCCAACT ACCAGTACCT GCTGCACCTC
    AACTCGCTGG CCGACCGCAG CTGCAGCGAC CTCTCCCAGT ACCCCGTGTT CCCCTGGGTC
    GTCGGCGACT ACAGCAGCTC CGAGCTGGAT CTGTCAAATC CAGAGACCTT CCGGGATCTC
    AGTAAGCCAG TAGGGGCCCT CAACCAGGAG CGTCTGGAGA GGCTGCTGAC CCGCTACCAG
    GAGATGCCGG AGCCCAGGTT CATGTACGGC AGCCACTACT CCTCCCCAGG CTACGTGCTC
    TTCTACCTGG TCCGAATCGC GCCAGAGTAC ATGCTGTGCC TGCAGAATGG AAGGTTCGAC
    AACGCAGACA GAATGTTCAA CAGTATTGCA GAAACTTGGA AAAACTGTTT GGATGGTGCA
    ACCGATTTTA AAGAGCTGAT CCCGGAATTC TACGGTGATG ATGTCAGCTT TTTAGTCAAC
    AGTCTGAAGT TGGATTTGGG GAAGAGACAA GGAGGGCAAC TGGTTAACGA CGTAGAGCTC
    CCGCCTTGGG CACGGAGTCC CGAGGATTTT CTCCGGAAGA GCAGAGCCGC GCTGGAGAGT
    GAGTATGTGT CCGAGCACCT GCATGAGTGG ATCGACCTGG TGTTTGGCTA CAAGCAGCAG
    GGCCGGGATG CCGTCGGAGC CCATAATGTG TTTCATCCGC TGACCTATGA AGGAGGTGTG
    GACTTGAACA GCATCGAGGA TCCTGACGAG AAGGTCGCCA TGCTCACCCA GATCTTAGAG
    TTCGGACAGA CACCCAGACA GCTCTTTGTG ACACCCCATC CTCGAAGAAT CACCCCAAAG
    TTTAAAAACG TGTCCCAGCC CTCCAGTTAC AATGCCTCCA CGGCAGATTC TCCAGGCGAA
    GAGTCTTTTG AAGACCTGAC AGAAGAGAGC AGAACCCTGG CCTGGAGTAA CATCGCCCGG
    CTAGAGCCCC AGGAGCAGCA CAGAATCCAC AAAGAGGCAG TCACTGGAAT AGCCGTGTCC
    CGCAATGGGT CGTCCATCTT CACAACCTCA CAAGATTCCA CCTTGAAGAT GTTTTCCAAA
    GAATCCAGGA TGCTACAGAG AAGCATATCA TTCTCAAATA TGGCTTTATC ATCCTGTTTG
    CTTTTACCAG GAGACGCCAC TGTCATAAGC TCTTCCTGGG ATAATAACGT CTATTTTTAT
    TCCATAGCAT TTGGAAGATG CCAGGACACA TTAATGGGAC ATGACGATGC TGTTAGTAAG
    ATCTGTTGGC ATGACAACAG GCTGTATTCT GCATCGTGGG ACTCTACTGT GAAGGTGTGG
    TCCGGGGTCC CCGCGGAGAT GCCGTGTGCC AGGAAACAGC ACTTTGAGCT GCTGGCTGAG
    CTGGAGCACG ACGTCAGCGT GGATACGATC AGTTTGAACG CTGCCGGCAC CCTGCTGGCC
    TCAGGCACCA AGGAAGGCAC AGTGACCATC TGGGACCTCA CCACGGCCAC CCTGTTACAC
    CAGATTCCAT GTCATGAGGG GACTGTAGGC GACACTGCTT TCAGCCCAGA CAGTCGCCAC
    ATCCTGAGCA CAGGAGCCAA CAGTCATCTG AACGTTATCG ACGTGCAGAC AGGAATGCCC
    ATCTCTTCTC TGACTGCAGA CGAGCCCCAG AGGTGCTTTC TCTGGGATGG AAACTCCGTC
    GTATCTGGGA GTCAGTCTGG GGAGCTGCTC ATCTGGGACC TCCTTGGGGG AAGGGTCAGC
    AAGAGGATCC AGGGCCACTC AGGCGCCGTG ACGTGCATGT GGATGAGCGA GCAGTGTAGC
    AGCGTCATCA CAGGAGGGGA AGACAGACAA ATTATGTTCT GGAAGTCGCA GTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006045717.1
    CDS1..3117
    Translation

    Target ORF information:

    RefSeq Version XM_006045655.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006045655.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGTCCT GTGGGCAGCC AGAAGTTAGC ATCGTCCCCC TGCTCCGGGG GCCTTATCCG 
    GGAAAAGCAG TGAAATGGAG CCGCGGGGCG GCGATTTGGG GCGGAGGGAC GGACCCCAGG
    CCGGGCCCTG GGTCCCGGGC GCCTCGGGCA AGCGCCCGCC CCTCCTCCGA CCTGAGTCAC
    GGCCGAGGCC CCACGCGGCG GGGATGCGGC GCTGAACGCG GGCTCGGGAC GCCAGGCCGC
    AGGCGCGCTC TCCTCGTGGG GCCCGCAGGC CCGCTGTGCC CGGCCGGCCT CCCGGGCTCC
    GGACCTCTCG GCCGGCGGCG GCGCCGCGGA ACCCGAGCTG GAGACGCGCG CCCCGCCGCC
    CTCCATGGCG TTCACCCGGA AGAGGCAGCG CGAGCAGCAG CTGCAGCTCT ACTCCAGGGA
    AGATTTTCCT TGCTTCTGCT TAACTTGGAG GAGTACTACT TTGAACAGCA CACGGCTCAT
    CACATTCAGC ACAAGGGCAG CCAGAAGGAA AGGAAAATCA GAGGCTCCTT AAAAATATGT
    TCAAAGTCAG TTATTTTTGA ACCGGATGCA ATATCCCAAC CCATCATTAA GATTCCTTTG
    AGGGATTGTA TAAAAATAGG AAGACATGGA GAAAATGAAG CCAGTAGACA CTTTGCAAAG
    ACAGTATCTG GGGCAATTTC ACTCATTTTC AGCCAGGTGT ATTTCATTAA AGAACACAAC
    ATTGTTGCAC CATATAAAAT AGAAAGGGGC ACGATGGAGT ATGTCTTTGA GCTGGACGTT
    TCAGGGAAAG TGGAAGATGT TGTGGAGACA CTCCTTCAAC TTCATAGAGC ATCCTGTCTG
    GACAAACTGG GGGACCAAAC TGCCATGATA ACAGCTATCT TGCAGTCCCG TTTGGCTAGG
    ACGTCATTCG ACAAAAACAG GTTCCAAAGT GTTTCTGAAA AGCTGCACAT GGAATGCAAA
    GCAGAAATGG TGACACCTCT GGTGACGAAC CCTGGCCACG CCTGCGTCAC TGACAGCACC
    CTCTATTTCC AGCCTCTCAA CGGGTACCCG AAACCCGTGG TCCAGATAAT GCTCCAGGAC
    ATCCGCCGCA TTTACAAGAG GAGACACGGC CTCATGCCTC TGGGCTTGGA AGTGTTTTGC
    ACAGAAGATG ACCTGTGTTC CGACATCTAC CTGAAGTTCT ATGAACGTCA GGACCGAGAC
    GACCTCTACT TTTACATCGC TACGTACCTA GAGCACCACG TGCCGGAGCG CACGGCCGAG
    GGCTACACGC TGCAGTGGCA GCGCGGCCTC CTGTCCAACT ACCAGTACCT GCTGCACCTC
    AACTCGCTGG CCGACCGCAG CTGCAGCGAC CTCTCCCAGT ACCCCGTGTT CCCCTGGGTC
    GTCGGCGACT ACAGCAGCTC CGAGCTGGAT CTGTCAAATC CAGAGACCTT CCGGGATCTC
    AGTAAGCCAG TAGGGGCCCT CAACCAGGAG CGTCTGGAGA GGCTGCTGAC CCGCTACCAG
    GAGATGCCGG AGCCCAGGTT CATGTACGGC AGCCACTACT CCTCCCCAGG CTACGTGCTC
    TTCTACCTGG TCCGAATCGC GCCAGAGTAC ATGCTGTGCC TGCAGAATGG AAGGTTCGAC
    AACGCAGACA GAATGTTCAA CAGTATTGCA GAAACTTGGA AAAACTGTTT GGATGGTGCA
    ACCGATTTTA AAGAGCTGAT CCCGGAATTC TACGGTGATG ATGTCAGCTT TTTAGTCAAC
    AGTCTGAAGT TGGATTTGGG GAAGAGACAA GGAGGGCAAC TGGTTAACGA CGTAGAGCTC
    CCGCCTTGGG CACGGAGTCC CGAGGATTTT CTCCGGAAGA GCAGAGCCGC GCTGGAGAGT
    GAGTATGTGT CCGAGCACCT GCATGAGTGG ATCGACCTGG TGTTTGGCTA CAAGCAGCAG
    GGCCGGGATG CCGTCGGAGC CCATAATGTG TTTCATCCGC TGACCTATGA AGGAGGTGTG
    GACTTGAACA GCATCGAGGA TCCTGACGAG AAGGTCGCCA TGCTCACCCA GATCTTAGAG
    TTCGGACAGA CACCCAGACA GCTCTTTGTG ACACCCCATC CTCGAAGAAT CACCCCAAAG
    TTTAAAAACG TGTCCCAGCC CTCCAGTTAC AATGCCTCCA CGGCAGATTC TCCAGGCGAA
    GAGTCTTTTG AAGACCTGAC AGAAGAGAGC AGAACCCTGG CCTGGAGTAA CATCGCCCGG
    CTAGAGCCCC AGGAGCAGCA CAGAATCCAC AAAGAGGCAG TCACTGGAAT AGCCGTGTCC
    CGCAATGGGT CGTCCATCTT CACAACCTCA CAAGATTCCA CCTTGAAGAT GTTTTCCAAA
    GAATCCAGGA TGCTACAGAG AAGCATATCA TTCTCAAATA TGGCTTTATC ATCCTGTTTG
    CTTTTACCAG GAGACGCCAC TGTCATAAGC TCTTCCTGGG ATAATAACGT CTATTTTTAT
    TCCATAGCAT TTGGAAGATG CCAGGACACA TTAATGGGAC ATGACGATGC TGTTAGTAAG
    ATCTGTTGGC ATGACAACAG GCTGTATTCT GCATCGTGGG ACTCTACTGT GAAGGTGTGG
    TCCGGGGTCC CCGCGGAGAT GCCGTGTGCC AGGAAACAGC ACTTTGAGCT GCTGGCTGAG
    CTGGAGCACG ACGTCAGCGT GGATACGATC AGTTTGAACG CTGCCGGCAC CCTGCTGGCC
    TCAGGCACCA AGGAAGGCAC AGTGACCATC TGGGACCTCA CCACGGCCAC CCTGTTACAC
    CAGATTCCAT GTCATGAGGG GACTGTAGGC GACACTGCTT TCAGCCCAGA CAGTCGCCAC
    ATCCTGAGCA CAGGAGCCAA CAGTCATCTG AACGTTATCG ACGTGCAGAC AGGAATGCCC
    ATCTCTTCTC TGACTGCAGA CGAGCCCCAG AGGTGCTTTC TCTGGGATGG AAACTCCGTC
    GTATCTGGGA GTCAGTCTGG GGAGCTGCTC ATCTGGGACC TCCTTGGGGG AAGGGTCAGC
    AAGAGGATCC AGGGCCACTC AGGCGCCGTG ACGTGCATGT GGATGAGCGA GCAGTGTAGC
    AGCGTCATCA CAGGAGGGGA AGACAGACAA ATTATGTTCT GGAAGTCGCA GTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.