TIAM1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIAM1
Entrez Gene ID	102413018
Full Name	T cell lymphoma invasion and metastasis 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006053558.1	XP_006053620.1	T-lymphoma invasion and metastasis-inducing protein 1 isoform X1
XM_025281782.1	XP_025137567.1	T-lymphoma invasion and metastasis-inducing protein 1 isoform X3
XM_006053560.1	XP_006053622.1	T-lymphoma invasion and metastasis-inducing protein 1 isoform X3
XM_006053558.2	XP_006053620.2	T-lymphoma invasion and metastasis-inducing protein 1 isoform X1
XM_025281775.1	XP_025137560.1	T-lymphoma invasion and metastasis-inducing protein 1 isoform X2
XM_006053559.1	XP_006053621.1	T-lymphoma invasion and metastasis-inducing protein 1 isoform X2
XM_006053559.2	XP_006053621.2	T-lymphoma invasion and metastasis-inducing protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following TIAM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIAM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu42526	XM_025281782.1 Latest version!	Bubalus bubalis T cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBu42525	XM_025281775.1 Latest version!	Bubalus bubalis T cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu11127	XM_006053558.1 Latest version!	Bubalus bubalis T-cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu11127	XM_006053559.1 Latest version!	Bubalus bubalis T-cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu11127	XM_006053560.1 Latest version!	Bubalus bubalis T-cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBu42524	XM_006053559.2 Latest version!	Bubalus bubalis T cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu42524	XM_006053558.2 Latest version!	Bubalus bubalis T cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIAM1 ( XM_025281782.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137567.1 Bubalus bubalis T cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu42526
Clone ID Related Accession (Same CDS sequence)	XM_025281782.1
Accession Version	XM_025281782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	T-lymphoma invasion and metastasis-inducing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCTG CATTGTGGAA AATAAAGCGC AAGCCCAGGA TTGAAAACAA TGGCAAGTCC 
AGCGATTCTG TATACCGGAC GCTTCATCTG AGCACGGAGC AGGTGGCCGC ATTCTGCCGT 
AGTCTGCACG AGATGAAGCC CTCTGACCCG AGCCCGTCCC CCCAGGACTG CACTGGGCCT 
CAGCTGGCCG CCCTGAGGCA GCTCACGGAT GCCGACAAGC TGCGCAAGGT CATCTGCGAG 
CTCCTGGAGA CAGAGCGCAC CTACGTGAAG GACTTAAACT GTCTCATGGA GAGATACCTA 
AAACCTCTTC AAAAAGAAAC TTTTCTCACC CAGGATGAGC TTGATGTGCT TTTTGGAAAT 
TTAACCGAAA TGGTAGAGTT TCAAGTAGAA TTCCTTAAAA CTCTGGAAGA TGGAGTGAGA 
TTGGTACCTG ATTTGGAAAA GCTTGAGAAA GTTGATCAAT TTAAGAAAGT GCTGTTCTCC 
CTGGGGGGAT CCTTTCTGTA TTACGCTGAC CGCTTCAAGC TCTACAGTGC CTTCTGCGCC 
AGCCACACGA AGGTCCCCAA GGTCCTGGTG AAAGCCAAGA CGGACGCAGC TTTCAAGGCG 
TTCCTGGACG CCCAGAACCC AAAGCAGCAG CACTCGTCCA CCCTGGAGTC TTACCTCATC 
AAGCCCATCC AGAGGATCCT TAAGTATCCG CTTCTGCTGA AGGAGCTTTT TGCTCTGACG 
GACGCGGAGA GTGAGGAACA CTACCACCTG GATGTGGCCA TCAAGACTAT GAACAAGGTT 
GCAAGTCATA TCAATGAAAT GCAGAAAATC CATGAGGAAT TTGGGGCTGT GTTTGACCAG 
CTGATTGCTG AACAGACCGG TGAGAAAAAA GAGGTTGCGG ATCTGAGCAT GGGAGACCTG 
CTGTTGCATA CCACCGTCAT CTGGCCAAAC CCGCCCGCCT CACTGGGCAA GTGGAAAAAG 
GAGCCTGAAT TGGCAGCATT TGTCTTCAAA ACTGCCGTGG TCCTTGTCTA TAAAGATGGT 
ACCAAACAGA AGAAGAAACT TGTAGGATCA CACAGGCTTT CAATTTACGA GGACTGGGAC 
CCCTTCCGAT TTCGACACAT GATTCCTACT GAAGCTCTGC AGGTTCGCGC TCTGGCAAGT 
GCAGATGCAG AAGCAAATGC CGTGTGTGAA ATCGTCCATG TAAAGTCGGA GTCGGAAGGG 
AGGCCGGAGA GGGTCTTCCA CCTGTGCTGC AGCTCCCCAG AGAGCCGAAA GGATTTCCTG 
AAGGCTGTGC ATTCCATCCT GCGAGATAAG CACAGAAGAC AGCTCCTGAA GACTGAGAGC 
CTTCCCTCGT CCCAGCAATA TGTCCCTTTC GGAGGAAAGC GGTTGTGTGC GCTGAAAGGG 
GCCAGGCCGG CTATGAGCAG GGCAGTGTCC GCCCCAAGCA AGTCTCTTGG GAGGAGGAGG 
CGGCGACTGG CCCGAAACAG GTTTACCATT GATTCCGATG CCATCTCCGC CAGCAGCCCG 
GAGAAAGACC CCCAGCAGCC CCCTGGTGGT GGGGACACTG ACCGCTGGGT GGAGGAACAG 
TTTGATCTTG CTCAGTATGA AGAGCAGGAT GACATCAAGG AGACAGACAT CCTCAGTGAC 
GATGATGAGT TCTGTGAGTC GGCCAAGGGC GCCTCGGCGG ACAGAGACCT GCAGGAGCGG 
CTTCAGGCTG CCTCCATCAC TCAGCGGGAG AAAGGCCGGA AGCCCCTGGA CAGCCACGCG 
TCCCGCATGA CGCAGCTCAA GAAGCAAGCC GCCCTGTCGG GCATCAACGG AGGCCCGGAG 
AGTACGAGCG AGGAGGTCAT TTGGGTTAGG CGCGAAGACT TTGCCCCCTC CCGCAAGCTG 
AACACTGAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137567.1
CDS	888..2762
Translation	MMSALWKIKRKPRIENNGKSSDSVYRTLHLSTEQVAAFCRSLHEMKPSDPSPSPQDCTGPQLAALRQLTDADKLRKVICELLETERTYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKTDAAFKAFLDAQNPKQQHSSTLESYLIKPIQRILKYPLLLKELFALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVADLSMGDLLLHTTVIWPNPPASLGKWKKEPELAAFVFKTAVVLVYKDGTKQKKKLVGSHRLSIYEDWDPFRFRHMIPTEALQVRALASADAEANAVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKAVHSILRDKHRRQLLKTESLPSSQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDAISASSPEKDPQQPPGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESAKGASADRDLQERLQAASITQREKGRKPLDSHASRMTQLKKQAALSGINGGPESTSEEVIWVRREDFAPSRKLNTEI

Target ORF information:

RefSeq Version	XM_025281782.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis T cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281782.1

ORF Insert Sequence:

ATGATGTCTG CATTGTGGAA AATAAAGCGC AAGCCCAGGA TTGAAAACAA TGGCAAGTCC 
AGCGATTCTG TATACCGGAC GCTTCATCTG AGCACGGAGC AGGTGGCCGC ATTCTGCCGT 
AGTCTGCACG AGATGAAGCC CTCTGACCCG AGCCCGTCCC CCCAGGACTG CACTGGGCCT 
CAGCTGGCCG CCCTGAGGCA GCTCACGGAT GCCGACAAGC TGCGCAAGGT CATCTGCGAG 
CTCCTGGAGA CAGAGCGCAC CTACGTGAAG GACTTAAACT GTCTCATGGA GAGATACCTA 
AAACCTCTTC AAAAAGAAAC TTTTCTCACC CAGGATGAGC TTGATGTGCT TTTTGGAAAT 
TTAACCGAAA TGGTAGAGTT TCAAGTAGAA TTCCTTAAAA CTCTGGAAGA TGGAGTGAGA 
TTGGTACCTG ATTTGGAAAA GCTTGAGAAA GTTGATCAAT TTAAGAAAGT GCTGTTCTCC 
CTGGGGGGAT CCTTTCTGTA TTACGCTGAC CGCTTCAAGC TCTACAGTGC CTTCTGCGCC 
AGCCACACGA AGGTCCCCAA GGTCCTGGTG AAAGCCAAGA CGGACGCAGC TTTCAAGGCG 
TTCCTGGACG CCCAGAACCC AAAGCAGCAG CACTCGTCCA CCCTGGAGTC TTACCTCATC 
AAGCCCATCC AGAGGATCCT TAAGTATCCG CTTCTGCTGA AGGAGCTTTT TGCTCTGACG 
GACGCGGAGA GTGAGGAACA CTACCACCTG GATGTGGCCA TCAAGACTAT GAACAAGGTT 
GCAAGTCATA TCAATGAAAT GCAGAAAATC CATGAGGAAT TTGGGGCTGT GTTTGACCAG 
CTGATTGCTG AACAGACCGG TGAGAAAAAA GAGGTTGCGG ATCTGAGCAT GGGAGACCTG 
CTGTTGCATA CCACCGTCAT CTGGCCAAAC CCGCCCGCCT CACTGGGCAA GTGGAAAAAG 
GAGCCTGAAT TGGCAGCATT TGTCTTCAAA ACTGCCGTGG TCCTTGTCTA TAAAGATGGT 
ACCAAACAGA AGAAGAAACT TGTAGGATCA CACAGGCTTT CAATTTACGA GGACTGGGAC 
CCCTTCCGAT TTCGACACAT GATTCCTACT GAAGCTCTGC AGGTTCGCGC TCTGGCAAGT 
GCAGATGCAG AAGCAAATGC CGTGTGTGAA ATCGTCCATG TAAAGTCGGA GTCGGAAGGG 
AGGCCGGAGA GGGTCTTCCA CCTGTGCTGC AGCTCCCCAG AGAGCCGAAA GGATTTCCTG 
AAGGCTGTGC ATTCCATCCT GCGAGATAAG CACAGAAGAC AGCTCCTGAA GACTGAGAGC 
CTTCCCTCGT CCCAGCAATA TGTCCCTTTC GGAGGAAAGC GGTTGTGTGC GCTGAAAGGG 
GCCAGGCCGG CTATGAGCAG GGCAGTGTCC GCCCCAAGCA AGTCTCTTGG GAGGAGGAGG 
CGGCGACTGG CCCGAAACAG GTTTACCATT GATTCCGATG CCATCTCCGC CAGCAGCCCG 
GAGAAAGACC CCCAGCAGCC CCCTGGTGGT GGGGACACTG ACCGCTGGGT GGAGGAACAG 
TTTGATCTTG CTCAGTATGA AGAGCAGGAT GACATCAAGG AGACAGACAT CCTCAGTGAC 
GATGATGAGT TCTGTGAGTC GGCCAAGGGC GCCTCGGCGG ACAGAGACCT GCAGGAGCGG 
CTTCAGGCTG CCTCCATCAC TCAGCGGGAG AAAGGCCGGA AGCCCCTGGA CAGCCACGCG 
TCCCGCATGA CGCAGCTCAA GAAGCAAGCC GCCCTGTCGG GCATCAACGG AGGCCCGGAG 
AGTACGAGCG AGGAGGTCAT TTGGGTTAGG CGCGAAGACT TTGCCCCCTC CCGCAAGCTG 
AACACTGAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIAM1 ( XM_025281775.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025137560.1 Bubalus bubalis T cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu42525
Clone ID Related Accession (Same CDS sequence)	XM_025281775.1
Accession Version	XM_025281775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	T-lymphoma invasion and metastasis-inducing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACAGT GCTCTTGGGG CAAGTTGGGC TGGGAACCGT TGTTGCCTTG TAAGGATGTT 
AACACAGGCG AGGGTAGCGA GTTTGCTGAC AGCGGGATCG AAGGGGCCAC CACCGACACG 
GACCTCCTGT CCCGGCGATC CAATGCCACT AACTCCAGCT ACTCGCCCCC CACCGGCCGT 
GCCTTTGTGG GCAGCGACAG CGGCAGCAGC TCCACCGGGG ACGCGGCCCG CCAGGGGGTG 
TATGAGAATT TTCGCCGGGA GCTGGAGATG AGCACCACGA ACAGCGAGAG CCTGGAGGAG 
GCGGGCTCCG CGCACAGCGA CGAGCAGAGC AGCGGCACCC TGAGCTCCCC GGGCCAGTCC 
GACATCCTGC TCACGGCCGC CCAGGGCACA GTGCGCAAGG CCGGCGCCCT GGCCGTCAAG 
AACTTCCTGG TGCACAAGAA GAACAAGAAG GTGGAGTCAG CCACGCGGAG GAAGTGGAAG 
CACTACTGGG TGTCCTTGAA AGGGTGCACA CTCTTCTTCT ATGAGGGCGA CGGCAGGTCC 
GGGATAGACC ACAACAGCGT CCCCAAGCAC GCCGTCTGGG TGGAGAACAG CATTGTACAG 
GCAGTTCCGG AGCACCCCAA GAAGGACTTC GTCTTCTGCC TCAGCAATTC CCTGGGCGAT 
GCCTTCCTCT TCCAGACCAC CAGCCAGACG GAGCTGGAGA ACTGGATCAC CGCCATCCAC 
TCTGCTTGCG CGGCCGCTGT CGCAAGACAC CACCACAAAG AGGACACGCT CCGGCTGCTG 
AAGTCGGAGA TCAAAAAACT GGAACAAAAG ATTGACATGG ACGAGAAGAT GAAGAAAATG 
GGCGAAATGC AGCTGTCTTC CGTCACGGAT TCAAAGAAGA AGAAAACTAT ATTAGATCAG 
ATCTTTGTCT GGGAGCAGAA TCTTGAGCAG TTCCAAATGG ACCTGTTTCG GTACCGCTGT 
TACCTAGCCA GCCTCCAAGG CGGAGAGCTG CCCAACCCTA AAAGGTTACT TGCCTTTGCA 
AGCAGACCAA CCAAAGTGGC AATGGGCCGC CTTGGAATTT TTTCCGTGTC ATCTTTTCAT 
GCCCTGGTGG CTGCCCGCAC TGGTGAAACT GGAGTCAGAA GACGTACTCA GGCCATGTCC 
CGATCTGCAA GCAAGAGAAG GAGCAGGTTT TCTTCTCTTT GGGGTCTGGA CACTACCTCT 
AAAAAGAAGC AGGGACGCCC AAGCATCAAT CAGGTGTTCG GTGAGGGAAC TGAGGCTGTA 
AAGAAATCTT TAGCGGGAGT ATTTGATGAC ACTGTTCCAG ATGGCAAGAG GGAGAAAGAA 
ATGGTCTTAC CTGCCGTCCA CCAGCACAAT CCTGACTGTG ACATTTGGGT CCACGAGTAT 
TTCACCCCAT CCTGGTTCTG TCTACCGAAC AATCAGCCAG CCCTGACGGT CGTCCGGCCG 
GGGGACACGG CCCGGGATAC CCTGGAGCTG ATCTGCAAGG CGCATAAACT GGATCATTCT 
GCTCATTACC TGCGCCTGAA ATTTCTAATA GAAAACAAAA TGCAGCATTA TGTGCCAAAG 
CCCGAGGAGG ACATTTATGA GCTGCTGTAC AAAGAAATTG AAATTTGTCC AAAAGTCACT 
CAGAACATCC AGATTGAGAA GTCAGATACA GCTGGTGATA ATTATGGGTT TTCACTTTCT 
TCTGTGGAAG AGGATGGTGT TCGAAGGCTC TACGTGAATA GTGTAAAAGA AACTGGCTTA 
GCTTCCAAGA AAGGCCTGAA GGCGGGAGAT GAGATTCTTG AGATCAATAA GCGGGCTGCA 
GGCACCCTGA ACTCCTCTGT GCTCAGAGAT TTCCTCACAC AGCCGTCCCT GGGCCTCCTG 
GTGAGGACCT GCCCCGAGCT GGAGGGAGGA GCAGAGCTGC TGGAGAACCC GCCCCATCGG 
CTGGACGGCC CGGCTGACCT CGGCGAGAGC CCCCTCGCCT TTCTCAGCAG TGACACAGGC 
CACAGCCTCT GCAGCGAGCA GGGCAGCAGT GCTGAGGCTG CTCCGGAGGA GGTGGAAGGG 
CCGGACTTGG AATCCTCAGA TGATACTGAT CACAGCAGCA AGAGCACGGA GCAGGTGGCC 
GCATTCTGCC GTAGTCTGCA CGAGATGAAG CCCTCTGACC CGAGCCCGTC CCCCCAGGAC 
TGCACTGGGC CTCAGCTGGC CGCCCTGAGG CAGCTCACGG ATGCCGACAA GCTGCGCAAG 
GTCATCTGCG AGCTCCTGGA GACAGAGCGC ACCTACGTGA AGGACTTAAA CTGTCTCATG 
GAGAGATACC TAAAACCTCT TCAAAAAGAA ACTTTTCTCA CCCAGGATGA GCTTGATGTG 
CTTTTTGGAA ATTTAACCGA AATGGTAGAG TTTCAAGTAG AATTCCTTAA AACTCTGGAA 
GATGGAGTGA GATTGGTACC TGATTTGGAA AAGCTTGAGA AAGTTGATCA ATTTAAGAAA 
GTGCTGTTCT CCCTGGGGGG ATCCTTTCTG TATTACGCTG ACCGCTTCAA GCTCTACAGT 
GCCTTCTGCG CCAGCCACAC GAAGGTCCCC AAGGTCCTGG TGAAAGCCAA GACGGACGCA 
GCTTTCAAGG CGTTCCTGGA CGCCCAGAAC CCAAAGCAGC AGCACTCGTC CACCCTGGAG 
TCTTACCTCA TCAAGCCCAT CCAGAGGATC CTTAAGTATC CGCTTCTGCT GAAGGAGCTT 
TTTGCTCTGA CGGACGCGGA GAGTGAGGAA CACTACCACC TGGATGTGGC CATCAAGACT 
ATGAACAAGG TTGCAAGTCA TATCAATGAA ATGCAGAAAA TCCATGAGGA ATTTGGGGCT 
GTGTTTGACC AGCTGATTGC TGAACAGACC GGTGAGAAAA AAGAGGTTGC GGATCTGAGC 
ATGGGAGACC TGCTGTTGCA TACCACCGTC ATCTGGCCAA ACCCGCCCGC CTCACTGGGC 
AAGTGGAAAA AGGAGCCTGA ATTGGCAGCA TTTGTCTTCA AAACTGCCGT GGTCCTTGTC 
TATAAAGATG GTACCAAACA GAAGAAGAAA CTTGTAGGAT CACACAGGCT TTCAATTTAC 
GAGGACTGGG ACCCCTTCCG ATTTCGACAC ATGATTCCTA CTGAAGCTCT GCAGGTTCGC 
GCTCTGGCAA GTGCAGATGC AGAAGCAAAT GCCGTGTGTG AAATCGTCCA TGTAAAGTCG 
GAGTCGGAAG GGAGGCCGGA GAGGGTCTTC CACCTGTGCT GCAGCTCCCC AGAGAGCCGA 
AAGGATTTCC TGAAGGCTGT GCATTCCATC CTGCGAGATA AGCACAGAAG ACAGCTCCTG 
AAGACTGAGA GCCTTCCCTC GTCCCAGCAA TATGTCCCTT TCGGAGGAAA GCGGTTGTGT 
GCGCTGAAAG GGGCCAGGCC GGCTATGAGC AGGGCAGTGT CCGCCCCAAG CAAGTCTCTT 
GGGAGGAGGA GGCGGCGACT GGCCCGAAAC AGGTTTACCA TTGATTCCGA TGCCATCTCC 
GCCAGCAGCC CGGAGAAAGA CCCCCAGCAG CCCCCTGGTG GTGGGGACAC TGACCGCTGG 
GTGGAGGAAC AGTTTGATCT TGCTCAGTAT GAAGAGCAGG ATGACATCAA GGAGACAGAC 
ATCCTCAGTG ACGATGATGA GTTCTGTGAG TCGGCCAAGG GCGCCTCGGC GGACAGAGAC 
CTGCAGGAGC GGCTTCAGGC TGCCTCCATC ACTCAGCGGG AGAAAGGCCG GAAGCCCCTG 
GACAGCCACG CGTCCCGCAT GACGCAGCTC AAGAAGCAAG CCGCCCTGTC GGGCATCAAC 
GGAGGCCCGG AGAGTACGAG CGAGGAGGTC ATTTGGGTTA GGCGCGAAGA CTTTGCCCCC 
TCCCGCAAGC TGAACACTGA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025137560.1
CDS	250..4116
Translation	MPQCSWGKLGWEPLLPCKDVNTGEGSEFADSGIEGATTDTDLLSRRSNATNSSYSPPTGRAFVGSDSGSSSTGDAARQGVYENFRRELEMSTTNSESLEEAGSAHSDEQSSGTLSSPGQSDILLTAAQGTVRKAGALAVKNFLVHKKNKKVESATRRKWKHYWVSLKGCTLFFYEGDGRSGIDHNSVPKHAVWVENSIVQAVPEHPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACAAAVARHHHKEDTLRLLKSEIKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQIFVWEQNLEQFQMDLFRYRCYLASLQGGELPNPKRLLAFASRPTKVAMGRLGIFSVSSFHALVAARTGETGVRRRTQAMSRSASKRRSRFSSLWGLDTTSKKKQGRPSINQVFGEGTEAVKKSLAGVFDDTVPDGKREKEMVLPAVHQHNPDCDIWVHEYFTPSWFCLPNNQPALTVVRPGDTARDTLELICKAHKLDHSAHYLRLKFLIENKMQHYVPKPEEDIYELLYKEIEICPKVTQNIQIEKSDTAGDNYGFSLSSVEEDGVRRLYVNSVKETGLASKKGLKAGDEILEINKRAAGTLNSSVLRDFLTQPSLGLLVRTCPELEGGAELLENPPHRLDGPADLGESPLAFLSSDTGHSLCSEQGSSAEAAPEEVEGPDLESSDDTDHSSKSTEQVAAFCRSLHEMKPSDPSPSPQDCTGPQLAALRQLTDADKLRKVICELLETERTYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKTDAAFKAFLDAQNPKQQHSSTLESYLIKPIQRILKYPLLLKELFALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVADLSMGDLLLHTTVIWPNPPASLGKWKKEPELAAFVFKTAVVLVYKDGTKQKKKLVGSHRLSIYEDWDPFRFRHMIPTEALQVRALASADAEANAVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKAVHSILRDKHRRQLLKTESLPSSQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDAISASSPEKDPQQPPGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESAKGASADRDLQERLQAASITQREKGRKPLDSHASRMTQLKKQAALSGINGGPESTSEEVIWVRREDFAPSRKLNTEI

Target ORF information:

RefSeq Version	XM_025281775.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis T cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025281775.1

ORF Insert Sequence:

ATGCCACAGT GCTCTTGGGG CAAGTTGGGC TGGGAACCGT TGTTGCCTTG TAAGGATGTT 
AACACAGGCG AGGGTAGCGA GTTTGCTGAC AGCGGGATCG AAGGGGCCAC CACCGACACG 
GACCTCCTGT CCCGGCGATC CAATGCCACT AACTCCAGCT ACTCGCCCCC CACCGGCCGT 
GCCTTTGTGG GCAGCGACAG CGGCAGCAGC TCCACCGGGG ACGCGGCCCG CCAGGGGGTG 
TATGAGAATT TTCGCCGGGA GCTGGAGATG AGCACCACGA ACAGCGAGAG CCTGGAGGAG 
GCGGGCTCCG CGCACAGCGA CGAGCAGAGC AGCGGCACCC TGAGCTCCCC GGGCCAGTCC 
GACATCCTGC TCACGGCCGC CCAGGGCACA GTGCGCAAGG CCGGCGCCCT GGCCGTCAAG 
AACTTCCTGG TGCACAAGAA GAACAAGAAG GTGGAGTCAG CCACGCGGAG GAAGTGGAAG 
CACTACTGGG TGTCCTTGAA AGGGTGCACA CTCTTCTTCT ATGAGGGCGA CGGCAGGTCC 
GGGATAGACC ACAACAGCGT CCCCAAGCAC GCCGTCTGGG TGGAGAACAG CATTGTACAG 
GCAGTTCCGG AGCACCCCAA GAAGGACTTC GTCTTCTGCC TCAGCAATTC CCTGGGCGAT 
GCCTTCCTCT TCCAGACCAC CAGCCAGACG GAGCTGGAGA ACTGGATCAC CGCCATCCAC 
TCTGCTTGCG CGGCCGCTGT CGCAAGACAC CACCACAAAG AGGACACGCT CCGGCTGCTG 
AAGTCGGAGA TCAAAAAACT GGAACAAAAG ATTGACATGG ACGAGAAGAT GAAGAAAATG 
GGCGAAATGC AGCTGTCTTC CGTCACGGAT TCAAAGAAGA AGAAAACTAT ATTAGATCAG 
ATCTTTGTCT GGGAGCAGAA TCTTGAGCAG TTCCAAATGG ACCTGTTTCG GTACCGCTGT 
TACCTAGCCA GCCTCCAAGG CGGAGAGCTG CCCAACCCTA AAAGGTTACT TGCCTTTGCA 
AGCAGACCAA CCAAAGTGGC AATGGGCCGC CTTGGAATTT TTTCCGTGTC ATCTTTTCAT 
GCCCTGGTGG CTGCCCGCAC TGGTGAAACT GGAGTCAGAA GACGTACTCA GGCCATGTCC 
CGATCTGCAA GCAAGAGAAG GAGCAGGTTT TCTTCTCTTT GGGGTCTGGA CACTACCTCT 
AAAAAGAAGC AGGGACGCCC AAGCATCAAT CAGGTGTTCG GTGAGGGAAC TGAGGCTGTA 
AAGAAATCTT TAGCGGGAGT ATTTGATGAC ACTGTTCCAG ATGGCAAGAG GGAGAAAGAA 
ATGGTCTTAC CTGCCGTCCA CCAGCACAAT CCTGACTGTG ACATTTGGGT CCACGAGTAT 
TTCACCCCAT CCTGGTTCTG TCTACCGAAC AATCAGCCAG CCCTGACGGT CGTCCGGCCG 
GGGGACACGG CCCGGGATAC CCTGGAGCTG ATCTGCAAGG CGCATAAACT GGATCATTCT 
GCTCATTACC TGCGCCTGAA ATTTCTAATA GAAAACAAAA TGCAGCATTA TGTGCCAAAG 
CCCGAGGAGG ACATTTATGA GCTGCTGTAC AAAGAAATTG AAATTTGTCC AAAAGTCACT 
CAGAACATCC AGATTGAGAA GTCAGATACA GCTGGTGATA ATTATGGGTT TTCACTTTCT 
TCTGTGGAAG AGGATGGTGT TCGAAGGCTC TACGTGAATA GTGTAAAAGA AACTGGCTTA 
GCTTCCAAGA AAGGCCTGAA GGCGGGAGAT GAGATTCTTG AGATCAATAA GCGGGCTGCA 
GGCACCCTGA ACTCCTCTGT GCTCAGAGAT TTCCTCACAC AGCCGTCCCT GGGCCTCCTG 
GTGAGGACCT GCCCCGAGCT GGAGGGAGGA GCAGAGCTGC TGGAGAACCC GCCCCATCGG 
CTGGACGGCC CGGCTGACCT CGGCGAGAGC CCCCTCGCCT TTCTCAGCAG TGACACAGGC 
CACAGCCTCT GCAGCGAGCA GGGCAGCAGT GCTGAGGCTG CTCCGGAGGA GGTGGAAGGG 
CCGGACTTGG AATCCTCAGA TGATACTGAT CACAGCAGCA AGAGCACGGA GCAGGTGGCC 
GCATTCTGCC GTAGTCTGCA CGAGATGAAG CCCTCTGACC CGAGCCCGTC CCCCCAGGAC 
TGCACTGGGC CTCAGCTGGC CGCCCTGAGG CAGCTCACGG ATGCCGACAA GCTGCGCAAG 
GTCATCTGCG AGCTCCTGGA GACAGAGCGC ACCTACGTGA AGGACTTAAA CTGTCTCATG 
GAGAGATACC TAAAACCTCT TCAAAAAGAA ACTTTTCTCA CCCAGGATGA GCTTGATGTG 
CTTTTTGGAA ATTTAACCGA AATGGTAGAG TTTCAAGTAG AATTCCTTAA AACTCTGGAA 
GATGGAGTGA GATTGGTACC TGATTTGGAA AAGCTTGAGA AAGTTGATCA ATTTAAGAAA 
GTGCTGTTCT CCCTGGGGGG ATCCTTTCTG TATTACGCTG ACCGCTTCAA GCTCTACAGT 
GCCTTCTGCG CCAGCCACAC GAAGGTCCCC AAGGTCCTGG TGAAAGCCAA GACGGACGCA 
GCTTTCAAGG CGTTCCTGGA CGCCCAGAAC CCAAAGCAGC AGCACTCGTC CACCCTGGAG 
TCTTACCTCA TCAAGCCCAT CCAGAGGATC CTTAAGTATC CGCTTCTGCT GAAGGAGCTT 
TTTGCTCTGA CGGACGCGGA GAGTGAGGAA CACTACCACC TGGATGTGGC CATCAAGACT 
ATGAACAAGG TTGCAAGTCA TATCAATGAA ATGCAGAAAA TCCATGAGGA ATTTGGGGCT 
GTGTTTGACC AGCTGATTGC TGAACAGACC GGTGAGAAAA AAGAGGTTGC GGATCTGAGC 
ATGGGAGACC TGCTGTTGCA TACCACCGTC ATCTGGCCAA ACCCGCCCGC CTCACTGGGC 
AAGTGGAAAA AGGAGCCTGA ATTGGCAGCA TTTGTCTTCA AAACTGCCGT GGTCCTTGTC 
TATAAAGATG GTACCAAACA GAAGAAGAAA CTTGTAGGAT CACACAGGCT TTCAATTTAC 
GAGGACTGGG ACCCCTTCCG ATTTCGACAC ATGATTCCTA CTGAAGCTCT GCAGGTTCGC 
GCTCTGGCAA GTGCAGATGC AGAAGCAAAT GCCGTGTGTG AAATCGTCCA TGTAAAGTCG 
GAGTCGGAAG GGAGGCCGGA GAGGGTCTTC CACCTGTGCT GCAGCTCCCC AGAGAGCCGA 
AAGGATTTCC TGAAGGCTGT GCATTCCATC CTGCGAGATA AGCACAGAAG ACAGCTCCTG 
AAGACTGAGA GCCTTCCCTC GTCCCAGCAA TATGTCCCTT TCGGAGGAAA GCGGTTGTGT 
GCGCTGAAAG GGGCCAGGCC GGCTATGAGC AGGGCAGTGT CCGCCCCAAG CAAGTCTCTT 
GGGAGGAGGA GGCGGCGACT GGCCCGAAAC AGGTTTACCA TTGATTCCGA TGCCATCTCC 
GCCAGCAGCC CGGAGAAAGA CCCCCAGCAG CCCCCTGGTG GTGGGGACAC TGACCGCTGG 
GTGGAGGAAC AGTTTGATCT TGCTCAGTAT GAAGAGCAGG ATGACATCAA GGAGACAGAC 
ATCCTCAGTG ACGATGATGA GTTCTGTGAG TCGGCCAAGG GCGCCTCGGC GGACAGAGAC 
CTGCAGGAGC GGCTTCAGGC TGCCTCCATC ACTCAGCGGG AGAAAGGCCG GAAGCCCCTG 
GACAGCCACG CGTCCCGCAT GACGCAGCTC AAGAAGCAAG CCGCCCTGTC GGGCATCAAC 
GGAGGCCCGG AGAGTACGAG CGAGGAGGTC ATTTGGGTTA GGCGCGAAGA CTTTGCCCCC 
TCCCGCAAGC TGAACACTGA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIAM1 ( XM_006053558.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053620.1 Bubalus bubalis T-cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu11127
Clone ID Related Accession (Same CDS sequence)	XM_006053558.1 , XM_006053560.1 , XM_006053559.1
Accession Version	XM_006053558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	T-lymphoma invasion and metastasis-inducing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784534.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACG CAGAAAGTCA AAATGTCGTG CATGAGTTCT ATGGAGACAA GCACGCCGTC 
CTGGGGCGCA AGCACACGTC GCGATCCCTG CGCCTGTCGC ACAAGGCGCG GCGGACCAGG 
CACGCCTCCT CCGGCAAGGT GATCCACAGG AACTCCGAAG TGAGTACCCG GTCCAGCAGC 
ACCCCCAGCA TCCCCCAGTC CCTGGCTGAA AATAGCCTGG AGCCCTTCTC CCAGGAAGGC 
ACCCTCGAGG ACTTCGGGAG CCCCATTTGG GTGGACCGAG TGGATATGGG CTTGAGACCC 
GTGTCTTACA CCGATTCCTC CGTCGCTCCC AGCGTGGACA GCAGCATCGT CCTCACAGCA 
GCCTCCGTGC AGAGCATGCC AGACTCGGAA GACAGCCGGC TTTATGGGGA TGACGCGACG 
TACTTGGCGG AGGGCGGCAG GCGGCAGCGC CCCTATGCCT CCAACGGACC CGCGTTCATG 
GAGACGGCGA GCTTTAAGAA GAAACGCTCC AAATCCGCAG ACATCTGGCG GGAGGACAGC 
CTGGAATTCT CACTGTCTGA TCTGAGCCAA GAACATTTAA CAAGCAACGA AGAGATCTTG 
GGTTCCGCCG AGGAGAAAGA CTGCGAGGAG GCTCGGGCGA TGGAAACCCG GGCGAGTCCG 
CGGCAGCTCA GTGCCTGTCA ACGGGCCAAT TCTCTGGGGG ACTTGTACGC TCAGAAGAAC 
CCTGGGGTGA CGGCAAACGG GGGGCCCAGG AGCAAACTTG CAGGCTACTG TCGGAATTTG 
GTGTCTGACA TCCCTGATCT CGCAAACCAT AAGATGCCAC CAGCTGCCGC TGAAGAGGCT 
CTCCCCTACA GTAATTATAA CACACTCCCC TGTAGGAAAT CTCACTGTCT CTCTGAAGGT 
GCCACCAACC CACAGATTAG CCATAGCAGC AGCATGCAAG GCAGAAGAGC AAAAACCACT 
CAGGATGTTA ACACAGGCGA GGGTAGCGAG TTTGCTGACA GCGGGATCGA AGGGGCCACC 
ACCGACACGG ACCTCCTGTC CCGGCGATCC AATGCCACTA ACTCCAGCTA CTCGCCCCCC 
ACCGGCCGTG CCTTTGTGGG CAGCGACAGC GGCAGCAGCT CCACCGGGGA CGCGGCCCGC 
CAGGGGGTGT ATGAGAATTT TCGCCGGGAG CTGGAGATGA GCACCACGAA CAGCGAGAGC 
CTGGAGGAGG CGGGCTCCGC GCACAGCGAC GAGCAGAGCA GCGGCACCCT GAGCTCCCCG 
GGCCAGTCCG ACATCCTGCT CACGGCCGCC CAGGGCACAG TGCGCAAGGC CGGCGCCCTG 
GCCGTCAAGA ACTTCCTGGT GCACAAGAAG AACAAGAAGG TGGAGTCAGC CACGCGGAGG 
AAGTGGAAGC ACTACTGGGT GTCCTTGAAA GGGTGCACAC TCTTCTTCTA CGAGGGCGAC 
GGCAGGTCTG GGATAGACCA CAACAGCGTC CCCAAGCACG CCGTCTGGGT GGAGAACAGC 
ATTGTACAGG CAGTTCCGGA GCACCCCAAG AAGGACTTCG TCTTCTGCCT CAGCAATTCC 
CTGGGCGATG CCTTCCTCTT CCAGACCACC AGCCAGACGG AGCTGGAGAA CTGGATCACC 
GCCATCCACT CTGCTTGCGC GGCCGCTGTC GCAAGACACC ACCACAAAGA GGACACGCTC 
CGGCTGCTGA AGTCGGAGAT CAAAAAACTG GAACAAAAGA TTGACATGGA CGAGAAGATG 
AAGAAAATGG GCGAAATGCA GCTGTCTTCC GTCACGGATT CAAAGAAGAA GAAAACTATA 
TTAGATCAGA TCTTTGTCTG GGAGCAGAAT CTTGAGCAGT TCCAAATGGA CCTGTTTCGG 
TACCGCTGTT ACCTAGCCAG CCTCCAAGGC GGAGAGCTGC CCAACCCTAA AAGGTTACTT 
GCCTTTGCAA GCAGACCAAC CAAAGTGGCA ATGGGCCGCC TTGGAATTTT TTCCGTGTCA 
TCTTTTCATG CCCTGGTGGC TGCCCGCACT GGTGAAACTG GAGTCAGAAG ACGTACTCAG 
GCCATGTCCC GATCTGCAAG CAAGAGAAGG AGCAGGTTTT CTTCTCTTTG GGGTCTGGAC 
ACTACCTCTA AAAAGAAGCA GGGACGCCCA AGCATCAATC AGGTGTTCGG TGAGGGAACT 
GAGGCTGTAA AGAAATCTTT AGCGGGAGTA TTTGATGACA CTGTTCCAGA TGGCAAGAGG 
GAGAAAGAAA TGGTCTTACC TGCCGTCCAC CAGCACAATC CTGACTGTGA CATTTGGGTC 
CACGAGTATT TCACCCCATC CTGGTTCTGT CTACCGAACA ATCAGCCAGC CCTGACGGTC 
GTCCGGCCGG GGGACACGGC CCGGGATACC CTGGAGCTGA TCTGCAAGGC GCATAAACTG 
GATCATTCTG CTCATTACCT GCGCCTGAAA TTTCTAATAG AAAACAAAAT GCAGCATTAT 
GTGCCAAAGC CCGAGGAGGA CATTTATGAG CTGCTGTACA AAGAAATTGA AATTTGTCCA 
AAAGTCACTC AGTACATCCA GATTGAGAAG TCAGATACAG CTGGTGATAA TTATGGGTTT 
TCACTTTCTT CTGTGGAAGA GGATGGTGTT CGAAGGCTCT ACGTGAATAG TGTAAAAGAA 
ACTGGCTTAG CTTCCAAGAA AGGCCTGAAG GCGGGAGATG AGATTCTTGA GATCAATAAG 
CGGGCTGCAG GCACCCTGAA CTCCTCTGTG CTCAGAGATT TCCTCACACA GCCGTCCCTG 
GGCCTCCTGG TGAGGACCTG CCCCGAGCTG GAGGGAGGAG CAGAGCTGCT GGAGAACCCG 
CCCCATCGGC TGGACGGCCC GGCTGACCTC GGCGAGAGCC CCCTCGCCTT TCTCAGCAGT 
GACACAGGCC ACAGCCTCTG CAGCGAGCAG GGCAGCAGTG CTGAGGCTGC TCCGGAGGAG 
GTGGAAGGGC CGGACTTGGA ATCCTCAGAT GATACTGATC ACAGCAGCAA GAGCACGGAG 
CAGGTGGCCG CATTCTGCCG TAGTCTGCAC GAGATGAAGC CCTCTGACCC GAGCCCGTCC 
CCCCAGGACT GCACTGGGCC TCAGCTGGCC GCCCTGAGGC AGCTCACGGA TGCCGACAAG 
CTGCGCAAGG TCATCTGCGA GCTCCTGGAG ACAGAGCGCA CCTACGTGAA GGACTTAAAC 
TGTCTCATGG AGAGATACCT AAAACCTCTT CAAAAAGAAA CTTTTCTCAC CCAGGATGAG 
CTTGATGTGC TTTTTGGAAA TTTAACCGAA ATGGTAGAGT TTCAAGTAGA ATTCCTTAAA 
ACTCTGGAAG ATGGAGTGAG ATTGGTACCT GATTTGGAAA AGCTTGAGAA AGTTGATCAA 
TTTAAGAAAG TGCTGTTCTC CCTGGGGGGA TCCTTTCTGT ATTACGCTGA CCGCTTCAAG 
CTCTACAGTG CCTTCTGCGC CAGCCACACG AAGGTCCCCA AGGTCCTGGT GAAAGCCAAG 
ACGGACGCAG CTTTCAAGGC GTTCCTGGAC GCCCAGAACC CAAAGCAGCA GCACTCGTCC 
ACCCTGGAGT CTTACCTCAT CAAGCCCATC CAGAGGATCC TTAAGTATCC GCTTCTGCTG 
AAGGAGCTTT TTGCTCTGAC GGACGCGGAG AGTGAGGAAC ACTACCACCT GGATGTGGCC 
ATCAAGACTA TGAACAAGGT TGCAAGTCAT ATCAATGAAA TGCAGAAAAT CCATGAGGAA 
TTTGGGGCTG TGTTTGACCA GCTGATTGCT GAACAGACCG GTGAGAAAAA AGAGGTTGCG 
GATCTGAGCA TGGGAGACCT GCTGTTGCAT ACCACCGTCA TCTGGCCAAA CCCGCCCGCC 
TCACTGGGCA AGTGGAAAAA GGAGCCTGAA TTGGCAGCAT TTGTCTTCAA AACTGCCGTG 
GTCCTTGTCT ATAAAGATGG TACCAAACAG AAGAAGAAAC TTGTAGGATC ACACAGGCTT 
TCAATTTACG AGGACTGGGA CCCCTTCCGA TTTCGACACA TGATTCCTAC TGAAGCTCTG 
CAGGTTCGCG CTCTGGCAAG TGCAGATGCA GAAGCAAATG CCGTGTGTGA AATCGTCCAT 
GTAAAGTCGG AGTCGGAAGG GAGGCCGGAG AGGGTCTTCC ACCTGTGCTG CAGCTCCCCA 
GAGAGCCGAA AGGATTTCCT GAAGGCTGTG CATTCCATCC TGCGAGATAA GCACAGAAGA 
CAGCTCCTGA AGACTGAGAG CCTTCCCTCG TCCCAGCAAT ATGTCCCTTT CGGAGGAAAG 
CGGTTGTGTG CGCTGAAAGG GGCCAGGCCG GCTATGAGCA GGGCAGTGTC CGCCCCAAGC 
AAGTCTCTTG GGAGGAGGAG GCGGCGACTG GCCCGAAACA GGTTTACCAT TGATTCCGAT 
GCCATCTCCG CCAGCAGCCC GGAGAAAGAC CCCCAGCAGC CCCCTGGTGG TGGGGACACT 
GACCGCTGGG TGGAGGAACA GTTTGATCTT GCTCAGTATG AAGAGCAGGA TGACATCAAG 
GAGACAGACA TCCTCAGTGA CGATGATGAG TTCTGTGAGT CGGCCAAGGG CGCCTCGGCG 
GACAGAGACC TGCAGGAGCG GCTTCAGGCT GCCTCCATCA CTCAGCGGGA GAAAGGCCGG 
AAGCCCCTGG ACAGCCACGC GTCCCGCATG ACGCAGCTCA AGAAGCAAGC CGCCCTGTCG 
GGCATCAACG GAGGCCCGGA GAGTACGAGC GAGGAGGTCA TTTGGGTTAG GCGCGAAGAC 
TTTGCCCCCT CCCGCAAGCT GAACACTGAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053620.1
CDS	320..5095
Translation	MGNAESQNVVHEFYGDKHAVLGRKHTSRSLRLSHKARRTRHASSGKVIHRNSEVSTRSSSTPSIPQSLAENSLEPFSQEGTLEDFGSPIWVDRVDMGLRPVSYTDSSVAPSVDSSIVLTAASVQSMPDSEDSRLYGDDATYLAEGGRRQRPYASNGPAFMETASFKKKRSKSADIWREDSLEFSLSDLSQEHLTSNEEILGSAEEKDCEEARAMETRASPRQLSACQRANSLGDLYAQKNPGVTANGGPRSKLAGYCRNLVSDIPDLANHKMPPAAAEEALPYSNYNTLPCRKSHCLSEGATNPQISHSSSMQGRRAKTTQDVNTGEGSEFADSGIEGATTDTDLLSRRSNATNSSYSPPTGRAFVGSDSGSSSTGDAARQGVYENFRRELEMSTTNSESLEEAGSAHSDEQSSGTLSSPGQSDILLTAAQGTVRKAGALAVKNFLVHKKNKKVESATRRKWKHYWVSLKGCTLFFYEGDGRSGIDHNSVPKHAVWVENSIVQAVPEHPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACAAAVARHHHKEDTLRLLKSEIKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQIFVWEQNLEQFQMDLFRYRCYLASLQGGELPNPKRLLAFASRPTKVAMGRLGIFSVSSFHALVAARTGETGVRRRTQAMSRSASKRRSRFSSLWGLDTTSKKKQGRPSINQVFGEGTEAVKKSLAGVFDDTVPDGKREKEMVLPAVHQHNPDCDIWVHEYFTPSWFCLPNNQPALTVVRPGDTARDTLELICKAHKLDHSAHYLRLKFLIENKMQHYVPKPEEDIYELLYKEIEICPKVTQYIQIEKSDTAGDNYGFSLSSVEEDGVRRLYVNSVKETGLASKKGLKAGDEILEINKRAAGTLNSSVLRDFLTQPSLGLLVRTCPELEGGAELLENPPHRLDGPADLGESPLAFLSSDTGHSLCSEQGSSAEAAPEEVEGPDLESSDDTDHSSKSTEQVAAFCRSLHEMKPSDPSPSPQDCTGPQLAALRQLTDADKLRKVICELLETERTYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKTDAAFKAFLDAQNPKQQHSSTLESYLIKPIQRILKYPLLLKELFALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVADLSMGDLLLHTTVIWPNPPASLGKWKKEPELAAFVFKTAVVLVYKDGTKQKKKLVGSHRLSIYEDWDPFRFRHMIPTEALQVRALASADAEANAVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKAVHSILRDKHRRQLLKTESLPSSQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDAISASSPEKDPQQPPGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESAKGASADRDLQERLQAASITQREKGRKPLDSHASRMTQLKKQAALSGINGGPESTSEEVIWVRREDFAPSRKLNTEI

Target ORF information:

RefSeq Version	XM_006053558.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis T-cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053558.1

ORF Insert Sequence:

ATGGGAAACG CAGAAAGTCA AAATGTCGTG CATGAGTTCT ATGGAGACAA GCACGCCGTC 
CTGGGGCGCA AGCACACGTC GCGATCCCTG CGCCTGTCGC ACAAGGCGCG GCGGACCAGG 
CACGCCTCCT CCGGCAAGGT GATCCACAGG AACTCCGAAG TGAGTACCCG GTCCAGCAGC 
ACCCCCAGCA TCCCCCAGTC CCTGGCTGAA AATAGCCTGG AGCCCTTCTC CCAGGAAGGC 
ACCCTCGAGG ACTTCGGGAG CCCCATTTGG GTGGACCGAG TGGATATGGG CTTGAGACCC 
GTGTCTTACA CCGATTCCTC CGTCGCTCCC AGCGTGGACA GCAGCATCGT CCTCACAGCA 
GCCTCCGTGC AGAGCATGCC AGACTCGGAA GACAGCCGGC TTTATGGGGA TGACGCGACG 
TACTTGGCGG AGGGCGGCAG GCGGCAGCGC CCCTATGCCT CCAACGGACC CGCGTTCATG 
GAGACGGCGA GCTTTAAGAA GAAACGCTCC AAATCCGCAG ACATCTGGCG GGAGGACAGC 
CTGGAATTCT CACTGTCTGA TCTGAGCCAA GAACATTTAA CAAGCAACGA AGAGATCTTG 
GGTTCCGCCG AGGAGAAAGA CTGCGAGGAG GCTCGGGCGA TGGAAACCCG GGCGAGTCCG 
CGGCAGCTCA GTGCCTGTCA ACGGGCCAAT TCTCTGGGGG ACTTGTACGC TCAGAAGAAC 
CCTGGGGTGA CGGCAAACGG GGGGCCCAGG AGCAAACTTG CAGGCTACTG TCGGAATTTG 
GTGTCTGACA TCCCTGATCT CGCAAACCAT AAGATGCCAC CAGCTGCCGC TGAAGAGGCT 
CTCCCCTACA GTAATTATAA CACACTCCCC TGTAGGAAAT CTCACTGTCT CTCTGAAGGT 
GCCACCAACC CACAGATTAG CCATAGCAGC AGCATGCAAG GCAGAAGAGC AAAAACCACT 
CAGGATGTTA ACACAGGCGA GGGTAGCGAG TTTGCTGACA GCGGGATCGA AGGGGCCACC 
ACCGACACGG ACCTCCTGTC CCGGCGATCC AATGCCACTA ACTCCAGCTA CTCGCCCCCC 
ACCGGCCGTG CCTTTGTGGG CAGCGACAGC GGCAGCAGCT CCACCGGGGA CGCGGCCCGC 
CAGGGGGTGT ATGAGAATTT TCGCCGGGAG CTGGAGATGA GCACCACGAA CAGCGAGAGC 
CTGGAGGAGG CGGGCTCCGC GCACAGCGAC GAGCAGAGCA GCGGCACCCT GAGCTCCCCG 
GGCCAGTCCG ACATCCTGCT CACGGCCGCC CAGGGCACAG TGCGCAAGGC CGGCGCCCTG 
GCCGTCAAGA ACTTCCTGGT GCACAAGAAG AACAAGAAGG TGGAGTCAGC CACGCGGAGG 
AAGTGGAAGC ACTACTGGGT GTCCTTGAAA GGGTGCACAC TCTTCTTCTA CGAGGGCGAC 
GGCAGGTCTG GGATAGACCA CAACAGCGTC CCCAAGCACG CCGTCTGGGT GGAGAACAGC 
ATTGTACAGG CAGTTCCGGA GCACCCCAAG AAGGACTTCG TCTTCTGCCT CAGCAATTCC 
CTGGGCGATG CCTTCCTCTT CCAGACCACC AGCCAGACGG AGCTGGAGAA CTGGATCACC 
GCCATCCACT CTGCTTGCGC GGCCGCTGTC GCAAGACACC ACCACAAAGA GGACACGCTC 
CGGCTGCTGA AGTCGGAGAT CAAAAAACTG GAACAAAAGA TTGACATGGA CGAGAAGATG 
AAGAAAATGG GCGAAATGCA GCTGTCTTCC GTCACGGATT CAAAGAAGAA GAAAACTATA 
TTAGATCAGA TCTTTGTCTG GGAGCAGAAT CTTGAGCAGT TCCAAATGGA CCTGTTTCGG 
TACCGCTGTT ACCTAGCCAG CCTCCAAGGC GGAGAGCTGC CCAACCCTAA AAGGTTACTT 
GCCTTTGCAA GCAGACCAAC CAAAGTGGCA ATGGGCCGCC TTGGAATTTT TTCCGTGTCA 
TCTTTTCATG CCCTGGTGGC TGCCCGCACT GGTGAAACTG GAGTCAGAAG ACGTACTCAG 
GCCATGTCCC GATCTGCAAG CAAGAGAAGG AGCAGGTTTT CTTCTCTTTG GGGTCTGGAC 
ACTACCTCTA AAAAGAAGCA GGGACGCCCA AGCATCAATC AGGTGTTCGG TGAGGGAACT 
GAGGCTGTAA AGAAATCTTT AGCGGGAGTA TTTGATGACA CTGTTCCAGA TGGCAAGAGG 
GAGAAAGAAA TGGTCTTACC TGCCGTCCAC CAGCACAATC CTGACTGTGA CATTTGGGTC 
CACGAGTATT TCACCCCATC CTGGTTCTGT CTACCGAACA ATCAGCCAGC CCTGACGGTC 
GTCCGGCCGG GGGACACGGC CCGGGATACC CTGGAGCTGA TCTGCAAGGC GCATAAACTG 
GATCATTCTG CTCATTACCT GCGCCTGAAA TTTCTAATAG AAAACAAAAT GCAGCATTAT 
GTGCCAAAGC CCGAGGAGGA CATTTATGAG CTGCTGTACA AAGAAATTGA AATTTGTCCA 
AAAGTCACTC AGTACATCCA GATTGAGAAG TCAGATACAG CTGGTGATAA TTATGGGTTT 
TCACTTTCTT CTGTGGAAGA GGATGGTGTT CGAAGGCTCT ACGTGAATAG TGTAAAAGAA 
ACTGGCTTAG CTTCCAAGAA AGGCCTGAAG GCGGGAGATG AGATTCTTGA GATCAATAAG 
CGGGCTGCAG GCACCCTGAA CTCCTCTGTG CTCAGAGATT TCCTCACACA GCCGTCCCTG 
GGCCTCCTGG TGAGGACCTG CCCCGAGCTG GAGGGAGGAG CAGAGCTGCT GGAGAACCCG 
CCCCATCGGC TGGACGGCCC GGCTGACCTC GGCGAGAGCC CCCTCGCCTT TCTCAGCAGT 
GACACAGGCC ACAGCCTCTG CAGCGAGCAG GGCAGCAGTG CTGAGGCTGC TCCGGAGGAG 
GTGGAAGGGC CGGACTTGGA ATCCTCAGAT GATACTGATC ACAGCAGCAA GAGCACGGAG 
CAGGTGGCCG CATTCTGCCG TAGTCTGCAC GAGATGAAGC CCTCTGACCC GAGCCCGTCC 
CCCCAGGACT GCACTGGGCC TCAGCTGGCC GCCCTGAGGC AGCTCACGGA TGCCGACAAG 
CTGCGCAAGG TCATCTGCGA GCTCCTGGAG ACAGAGCGCA CCTACGTGAA GGACTTAAAC 
TGTCTCATGG AGAGATACCT AAAACCTCTT CAAAAAGAAA CTTTTCTCAC CCAGGATGAG 
CTTGATGTGC TTTTTGGAAA TTTAACCGAA ATGGTAGAGT TTCAAGTAGA ATTCCTTAAA 
ACTCTGGAAG ATGGAGTGAG ATTGGTACCT GATTTGGAAA AGCTTGAGAA AGTTGATCAA 
TTTAAGAAAG TGCTGTTCTC CCTGGGGGGA TCCTTTCTGT ATTACGCTGA CCGCTTCAAG 
CTCTACAGTG CCTTCTGCGC CAGCCACACG AAGGTCCCCA AGGTCCTGGT GAAAGCCAAG 
ACGGACGCAG CTTTCAAGGC GTTCCTGGAC GCCCAGAACC CAAAGCAGCA GCACTCGTCC 
ACCCTGGAGT CTTACCTCAT CAAGCCCATC CAGAGGATCC TTAAGTATCC GCTTCTGCTG 
AAGGAGCTTT TTGCTCTGAC GGACGCGGAG AGTGAGGAAC ACTACCACCT GGATGTGGCC 
ATCAAGACTA TGAACAAGGT TGCAAGTCAT ATCAATGAAA TGCAGAAAAT CCATGAGGAA 
TTTGGGGCTG TGTTTGACCA GCTGATTGCT GAACAGACCG GTGAGAAAAA AGAGGTTGCG 
GATCTGAGCA TGGGAGACCT GCTGTTGCAT ACCACCGTCA TCTGGCCAAA CCCGCCCGCC 
TCACTGGGCA AGTGGAAAAA GGAGCCTGAA TTGGCAGCAT TTGTCTTCAA AACTGCCGTG 
GTCCTTGTCT ATAAAGATGG TACCAAACAG AAGAAGAAAC TTGTAGGATC ACACAGGCTT 
TCAATTTACG AGGACTGGGA CCCCTTCCGA TTTCGACACA TGATTCCTAC TGAAGCTCTG 
CAGGTTCGCG CTCTGGCAAG TGCAGATGCA GAAGCAAATG CCGTGTGTGA AATCGTCCAT 
GTAAAGTCGG AGTCGGAAGG GAGGCCGGAG AGGGTCTTCC ACCTGTGCTG CAGCTCCCCA 
GAGAGCCGAA AGGATTTCCT GAAGGCTGTG CATTCCATCC TGCGAGATAA GCACAGAAGA 
CAGCTCCTGA AGACTGAGAG CCTTCCCTCG TCCCAGCAAT ATGTCCCTTT CGGAGGAAAG 
CGGTTGTGTG CGCTGAAAGG GGCCAGGCCG GCTATGAGCA GGGCAGTGTC CGCCCCAAGC 
AAGTCTCTTG GGAGGAGGAG GCGGCGACTG GCCCGAAACA GGTTTACCAT TGATTCCGAT 
GCCATCTCCG CCAGCAGCCC GGAGAAAGAC CCCCAGCAGC CCCCTGGTGG TGGGGACACT 
GACCGCTGGG TGGAGGAACA GTTTGATCTT GCTCAGTATG AAGAGCAGGA TGACATCAAG 
GAGACAGACA TCCTCAGTGA CGATGATGAG TTCTGTGAGT CGGCCAAGGG CGCCTCGGCG 
GACAGAGACC TGCAGGAGCG GCTTCAGGCT GCCTCCATCA CTCAGCGGGA GAAAGGCCGG 
AAGCCCCTGG ACAGCCACGC GTCCCGCATG ACGCAGCTCA AGAAGCAAGC CGCCCTGTCG 
GGCATCAACG GAGGCCCGGA GAGTACGAGC GAGGAGGTCA TTTGGGTTAG GCGCGAAGAC 
TTTGCCCCCT CCCGCAAGCT GAACACTGAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIAM1 ( XM_006053560.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053622.1 Bubalus bubalis T-cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu11127
Clone ID Related Accession (Same CDS sequence)	XM_006053558.1 , XM_006053560.1 , XM_006053559.1
Accession Version	XM_006053560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	T-lymphoma invasion and metastasis-inducing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784534.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACG CAGAAAGTCA AAATGTCGTG CATGAGTTCT ATGGAGACAA GCACGCCGTC 
CTGGGGCGCA AGCACACGTC GCGATCCCTG CGCCTGTCGC ACAAGGCGCG GCGGACCAGG 
CACGCCTCCT CCGGCAAGGT GATCCACAGG AACTCCGAAG TGAGTACCCG GTCCAGCAGC 
ACCCCCAGCA TCCCCCAGTC CCTGGCTGAA AATAGCCTGG AGCCCTTCTC CCAGGAAGGC 
ACCCTCGAGG ACTTCGGGAG CCCCATTTGG GTGGACCGAG TGGATATGGG CTTGAGACCC 
GTGTCTTACA CCGATTCCTC CGTCGCTCCC AGCGTGGACA GCAGCATCGT CCTCACAGCA 
GCCTCCGTGC AGAGCATGCC AGACTCGGAA GACAGCCGGC TTTATGGGGA TGACGCGACG 
TACTTGGCGG AGGGCGGCAG GCGGCAGCGC CCCTATGCCT CCAACGGACC CGCGTTCATG 
GAGACGGCGA GCTTTAAGAA GAAACGCTCC AAATCCGCAG ACATCTGGCG GGAGGACAGC 
CTGGAATTCT CACTGTCTGA TCTGAGCCAA GAACATTTAA CAAGCAACGA AGAGATCTTG 
GGTTCCGCCG AGGAGAAAGA CTGCGAGGAG GCTCGGGCGA TGGAAACCCG GGCGAGTCCG 
CGGCAGCTCA GTGCCTGTCA ACGGGCCAAT TCTCTGGGGG ACTTGTACGC TCAGAAGAAC 
CCTGGGGTGA CGGCAAACGG GGGGCCCAGG AGCAAACTTG CAGGCTACTG TCGGAATTTG 
GTGTCTGACA TCCCTGATCT CGCAAACCAT AAGATGCCAC CAGCTGCCGC TGAAGAGGCT 
CTCCCCTACA GTAATTATAA CACACTCCCC TGTAGGAAAT CTCACTGTCT CTCTGAAGGT 
GCCACCAACC CACAGATTAG CCATAGCAGC AGCATGCAAG GCAGAAGAGC AAAAACCACT 
CAGGATGTTA ACACAGGCGA GGGTAGCGAG TTTGCTGACA GCGGGATCGA AGGGGCCACC 
ACCGACACGG ACCTCCTGTC CCGGCGATCC AATGCCACTA ACTCCAGCTA CTCGCCCCCC 
ACCGGCCGTG CCTTTGTGGG CAGCGACAGC GGCAGCAGCT CCACCGGGGA CGCGGCCCGC 
CAGGGGGTGT ATGAGAATTT TCGCCGGGAG CTGGAGATGA GCACCACGAA CAGCGAGAGC 
CTGGAGGAGG CGGGCTCCGC GCACAGCGAC GAGCAGAGCA GCGGCACCCT GAGCTCCCCG 
GGCCAGTCCG ACATCCTGCT CACGGCCGCC CAGGGCACAG TGCGCAAGGC CGGCGCCCTG 
GCCGTCAAGA ACTTCCTGGT GCACAAGAAG AACAAGAAGG TGGAGTCAGC CACGCGGAGG 
AAGTGGAAGC ACTACTGGGT GTCCTTGAAA GGGTGCACAC TCTTCTTCTA CGAGGGCGAC 
GGCAGGTCTG GGATAGACCA CAACAGCGTC CCCAAGCACG CCGTCTGGGT GGAGAACAGC 
ATTGTACAGG CAGTTCCGGA GCACCCCAAG AAGGACTTCG TCTTCTGCCT CAGCAATTCC 
CTGGGCGATG CCTTCCTCTT CCAGACCACC AGCCAGACGG AGCTGGAGAA CTGGATCACC 
GCCATCCACT CTGCTTGCGC GGCCGCTGTC GCAAGACACC ACCACAAAGA GGACACGCTC 
CGGCTGCTGA AGTCGGAGAT CAAAAAACTG GAACAAAAGA TTGACATGGA CGAGAAGATG 
AAGAAAATGG GCGAAATGCA GCTGTCTTCC GTCACGGATT CAAAGAAGAA GAAAACTATA 
TTAGATCAGA TCTTTGTCTG GGAGCAGAAT CTTGAGCAGT TCCAAATGGA CCTGTTTCGG 
TACCGCTGTT ACCTAGCCAG CCTCCAAGGC GGAGAGCTGC CCAACCCTAA AAGGTTACTT 
GCCTTTGCAA GCAGACCAAC CAAAGTGGCA ATGGGCCGCC TTGGAATTTT TTCCGTGTCA 
TCTTTTCATG CCCTGGTGGC TGCCCGCACT GGTGAAACTG GAGTCAGAAG ACGTACTCAG 
GCCATGTCCC GATCTGCAAG CAAGAGAAGG AGCAGGTTTT CTTCTCTTTG GGGTCTGGAC 
ACTACCTCTA AAAAGAAGCA GGGACGCCCA AGCATCAATC AGGTGTTCGG TGAGGGAACT 
GAGGCTGTAA AGAAATCTTT AGCGGGAGTA TTTGATGACA CTGTTCCAGA TGGCAAGAGG 
GAGAAAGAAA TGGTCTTACC TGCCGTCCAC CAGCACAATC CTGACTGTGA CATTTGGGTC 
CACGAGTATT TCACCCCATC CTGGTTCTGT CTACCGAACA ATCAGCCAGC CCTGACGGTC 
GTCCGGCCGG GGGACACGGC CCGGGATACC CTGGAGCTGA TCTGCAAGGC GCATAAACTG 
GATCATTCTG CTCATTACCT GCGCCTGAAA TTTCTAATAG AAAACAAAAT GCAGCATTAT 
GTGCCAAAGC CCGAGGAGGA CATTTATGAG CTGCTGTACA AAGAAATTGA AATTTGTCCA 
AAAGTCACTC AGTACATCCA GATTGAGAAG TCAGATACAG CTGGTGATAA TTATGGGTTT 
TCACTTTCTT CTGTGGAAGA GGATGGTGTT CGAAGGCTCT ACGTGAATAG TGTAAAAGAA 
ACTGGCTTAG CTTCCAAGAA AGGCCTGAAG GCGGGAGATG AGATTCTTGA GATCAATAAG 
CGGGCTGCAG GCACCCTGAA CTCCTCTGTG CTCAGAGATT TCCTCACACA GCCGTCCCTG 
GGCCTCCTGG TGAGGACCTG CCCCGAGCTG GAGGGAGGAG CAGAGCTGCT GGAGAACCCG 
CCCCATCGGC TGGACGGCCC GGCTGACCTC GGCGAGAGCC CCCTCGCCTT TCTCAGCAGT 
GACACAGGCC ACAGCCTCTG CAGCGAGCAG GGCAGCAGTG CTGAGGCTGC TCCGGAGGAG 
GTGGAAGGGC CGGACTTGGA ATCCTCAGAT GATACTGATC ACAGCAGCAA GAGCACGGAG 
CAGGTGGCCG CATTCTGCCG TAGTCTGCAC GAGATGAAGC CCTCTGACCC GAGCCCGTCC 
CCCCAGGACT GCACTGGGCC TCAGCTGGCC GCCCTGAGGC AGCTCACGGA TGCCGACAAG 
CTGCGCAAGG TCATCTGCGA GCTCCTGGAG ACAGAGCGCA CCTACGTGAA GGACTTAAAC 
TGTCTCATGG AGAGATACCT AAAACCTCTT CAAAAAGAAA CTTTTCTCAC CCAGGATGAG 
CTTGATGTGC TTTTTGGAAA TTTAACCGAA ATGGTAGAGT TTCAAGTAGA ATTCCTTAAA 
ACTCTGGAAG ATGGAGTGAG ATTGGTACCT GATTTGGAAA AGCTTGAGAA AGTTGATCAA 
TTTAAGAAAG TGCTGTTCTC CCTGGGGGGA TCCTTTCTGT ATTACGCTGA CCGCTTCAAG 
CTCTACAGTG CCTTCTGCGC CAGCCACACG AAGGTCCCCA AGGTCCTGGT GAAAGCCAAG 
ACGGACGCAG CTTTCAAGGC GTTCCTGGAC GCCCAGAACC CAAAGCAGCA GCACTCGTCC 
ACCCTGGAGT CTTACCTCAT CAAGCCCATC CAGAGGATCC TTAAGTATCC GCTTCTGCTG 
AAGGAGCTTT TTGCTCTGAC GGACGCGGAG AGTGAGGAAC ACTACCACCT GGATGTGGCC 
ATCAAGACTA TGAACAAGGT TGCAAGTCAT ATCAATGAAA TGCAGAAAAT CCATGAGGAA 
TTTGGGGCTG TGTTTGACCA GCTGATTGCT GAACAGACCG GTGAGAAAAA AGAGGTTGCG 
GATCTGAGCA TGGGAGACCT GCTGTTGCAT ACCACCGTCA TCTGGCCAAA CCCGCCCGCC 
TCACTGGGCA AGTGGAAAAA GGAGCCTGAA TTGGCAGCAT TTGTCTTCAA AACTGCCGTG 
GTCCTTGTCT ATAAAGATGG TACCAAACAG AAGAAGAAAC TTGTAGGATC ACACAGGCTT 
TCAATTTACG AGGACTGGGA CCCCTTCCGA TTTCGACACA TGATTCCTAC TGAAGCTCTG 
CAGGTTCGCG CTCTGGCAAG TGCAGATGCA GAAGCAAATG CCGTGTGTGA AATCGTCCAT 
GTAAAGTCGG AGTCGGAAGG GAGGCCGGAG AGGGTCTTCC ACCTGTGCTG CAGCTCCCCA 
GAGAGCCGAA AGGATTTCCT GAAGGCTGTG CATTCCATCC TGCGAGATAA GCACAGAAGA 
CAGCTCCTGA AGACTGAGAG CCTTCCCTCG TCCCAGCAAT ATGTCCCTTT CGGAGGAAAG 
CGGTTGTGTG CGCTGAAAGG GGCCAGGCCG GCTATGAGCA GGGCAGTGTC CGCCCCAAGC 
AAGTCTCTTG GGAGGAGGAG GCGGCGACTG GCCCGAAACA GGTTTACCAT TGATTCCGAT 
GCCATCTCCG CCAGCAGCCC GGAGAAAGAC CCCCAGCAGC CCCCTGGTGG TGGGGACACT 
GACCGCTGGG TGGAGGAACA GTTTGATCTT GCTCAGTATG AAGAGCAGGA TGACATCAAG 
GAGACAGACA TCCTCAGTGA CGATGATGAG TTCTGTGAGT CGGCCAAGGG CGCCTCGGCG 
GACAGAGACC TGCAGGAGCG GCTTCAGGCT GCCTCCATCA CTCAGCGGGA GAAAGGCCGG 
AAGCCCCTGG ACAGCCACGC GTCCCGCATG ACGCAGCTCA AGAAGCAAGC CGCCCTGTCG 
GGCATCAACG GAGGCCCGGA GAGTACGAGC GAGGAGGTCA TTTGGGTTAG GCGCGAAGAC 
TTTGCCCCCT CCCGCAAGCT GAACACTGAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053622.1
CDS	260..5035
Translation	MGNAESQNVVHEFYGDKHAVLGRKHTSRSLRLSHKARRTRHASSGKVIHRNSEVSTRSSSTPSIPQSLAENSLEPFSQEGTLEDFGSPIWVDRVDMGLRPVSYTDSSVAPSVDSSIVLTAASVQSMPDSEDSRLYGDDATYLAEGGRRQRPYASNGPAFMETASFKKKRSKSADIWREDSLEFSLSDLSQEHLTSNEEILGSAEEKDCEEARAMETRASPRQLSACQRANSLGDLYAQKNPGVTANGGPRSKLAGYCRNLVSDIPDLANHKMPPAAAEEALPYSNYNTLPCRKSHCLSEGATNPQISHSSSMQGRRAKTTQDVNTGEGSEFADSGIEGATTDTDLLSRRSNATNSSYSPPTGRAFVGSDSGSSSTGDAARQGVYENFRRELEMSTTNSESLEEAGSAHSDEQSSGTLSSPGQSDILLTAAQGTVRKAGALAVKNFLVHKKNKKVESATRRKWKHYWVSLKGCTLFFYEGDGRSGIDHNSVPKHAVWVENSIVQAVPEHPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACAAAVARHHHKEDTLRLLKSEIKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQIFVWEQNLEQFQMDLFRYRCYLASLQGGELPNPKRLLAFASRPTKVAMGRLGIFSVSSFHALVAARTGETGVRRRTQAMSRSASKRRSRFSSLWGLDTTSKKKQGRPSINQVFGEGTEAVKKSLAGVFDDTVPDGKREKEMVLPAVHQHNPDCDIWVHEYFTPSWFCLPNNQPALTVVRPGDTARDTLELICKAHKLDHSAHYLRLKFLIENKMQHYVPKPEEDIYELLYKEIEICPKVTQYIQIEKSDTAGDNYGFSLSSVEEDGVRRLYVNSVKETGLASKKGLKAGDEILEINKRAAGTLNSSVLRDFLTQPSLGLLVRTCPELEGGAELLENPPHRLDGPADLGESPLAFLSSDTGHSLCSEQGSSAEAAPEEVEGPDLESSDDTDHSSKSTEQVAAFCRSLHEMKPSDPSPSPQDCTGPQLAALRQLTDADKLRKVICELLETERTYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKTDAAFKAFLDAQNPKQQHSSTLESYLIKPIQRILKYPLLLKELFALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVADLSMGDLLLHTTVIWPNPPASLGKWKKEPELAAFVFKTAVVLVYKDGTKQKKKLVGSHRLSIYEDWDPFRFRHMIPTEALQVRALASADAEANAVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKAVHSILRDKHRRQLLKTESLPSSQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDAISASSPEKDPQQPPGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESAKGASADRDLQERLQAASITQREKGRKPLDSHASRMTQLKKQAALSGINGGPESTSEEVIWVRREDFAPSRKLNTEI

Target ORF information:

RefSeq Version	XM_006053560.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis T-cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053560.1

ORF Insert Sequence:

ATGGGAAACG CAGAAAGTCA AAATGTCGTG CATGAGTTCT ATGGAGACAA GCACGCCGTC 
CTGGGGCGCA AGCACACGTC GCGATCCCTG CGCCTGTCGC ACAAGGCGCG GCGGACCAGG 
CACGCCTCCT CCGGCAAGGT GATCCACAGG AACTCCGAAG TGAGTACCCG GTCCAGCAGC 
ACCCCCAGCA TCCCCCAGTC CCTGGCTGAA AATAGCCTGG AGCCCTTCTC CCAGGAAGGC 
ACCCTCGAGG ACTTCGGGAG CCCCATTTGG GTGGACCGAG TGGATATGGG CTTGAGACCC 
GTGTCTTACA CCGATTCCTC CGTCGCTCCC AGCGTGGACA GCAGCATCGT CCTCACAGCA 
GCCTCCGTGC AGAGCATGCC AGACTCGGAA GACAGCCGGC TTTATGGGGA TGACGCGACG 
TACTTGGCGG AGGGCGGCAG GCGGCAGCGC CCCTATGCCT CCAACGGACC CGCGTTCATG 
GAGACGGCGA GCTTTAAGAA GAAACGCTCC AAATCCGCAG ACATCTGGCG GGAGGACAGC 
CTGGAATTCT CACTGTCTGA TCTGAGCCAA GAACATTTAA CAAGCAACGA AGAGATCTTG 
GGTTCCGCCG AGGAGAAAGA CTGCGAGGAG GCTCGGGCGA TGGAAACCCG GGCGAGTCCG 
CGGCAGCTCA GTGCCTGTCA ACGGGCCAAT TCTCTGGGGG ACTTGTACGC TCAGAAGAAC 
CCTGGGGTGA CGGCAAACGG GGGGCCCAGG AGCAAACTTG CAGGCTACTG TCGGAATTTG 
GTGTCTGACA TCCCTGATCT CGCAAACCAT AAGATGCCAC CAGCTGCCGC TGAAGAGGCT 
CTCCCCTACA GTAATTATAA CACACTCCCC TGTAGGAAAT CTCACTGTCT CTCTGAAGGT 
GCCACCAACC CACAGATTAG CCATAGCAGC AGCATGCAAG GCAGAAGAGC AAAAACCACT 
CAGGATGTTA ACACAGGCGA GGGTAGCGAG TTTGCTGACA GCGGGATCGA AGGGGCCACC 
ACCGACACGG ACCTCCTGTC CCGGCGATCC AATGCCACTA ACTCCAGCTA CTCGCCCCCC 
ACCGGCCGTG CCTTTGTGGG CAGCGACAGC GGCAGCAGCT CCACCGGGGA CGCGGCCCGC 
CAGGGGGTGT ATGAGAATTT TCGCCGGGAG CTGGAGATGA GCACCACGAA CAGCGAGAGC 
CTGGAGGAGG CGGGCTCCGC GCACAGCGAC GAGCAGAGCA GCGGCACCCT GAGCTCCCCG 
GGCCAGTCCG ACATCCTGCT CACGGCCGCC CAGGGCACAG TGCGCAAGGC CGGCGCCCTG 
GCCGTCAAGA ACTTCCTGGT GCACAAGAAG AACAAGAAGG TGGAGTCAGC CACGCGGAGG 
AAGTGGAAGC ACTACTGGGT GTCCTTGAAA GGGTGCACAC TCTTCTTCTA CGAGGGCGAC 
GGCAGGTCTG GGATAGACCA CAACAGCGTC CCCAAGCACG CCGTCTGGGT GGAGAACAGC 
ATTGTACAGG CAGTTCCGGA GCACCCCAAG AAGGACTTCG TCTTCTGCCT CAGCAATTCC 
CTGGGCGATG CCTTCCTCTT CCAGACCACC AGCCAGACGG AGCTGGAGAA CTGGATCACC 
GCCATCCACT CTGCTTGCGC GGCCGCTGTC GCAAGACACC ACCACAAAGA GGACACGCTC 
CGGCTGCTGA AGTCGGAGAT CAAAAAACTG GAACAAAAGA TTGACATGGA CGAGAAGATG 
AAGAAAATGG GCGAAATGCA GCTGTCTTCC GTCACGGATT CAAAGAAGAA GAAAACTATA 
TTAGATCAGA TCTTTGTCTG GGAGCAGAAT CTTGAGCAGT TCCAAATGGA CCTGTTTCGG 
TACCGCTGTT ACCTAGCCAG CCTCCAAGGC GGAGAGCTGC CCAACCCTAA AAGGTTACTT 
GCCTTTGCAA GCAGACCAAC CAAAGTGGCA ATGGGCCGCC TTGGAATTTT TTCCGTGTCA 
TCTTTTCATG CCCTGGTGGC TGCCCGCACT GGTGAAACTG GAGTCAGAAG ACGTACTCAG 
GCCATGTCCC GATCTGCAAG CAAGAGAAGG AGCAGGTTTT CTTCTCTTTG GGGTCTGGAC 
ACTACCTCTA AAAAGAAGCA GGGACGCCCA AGCATCAATC AGGTGTTCGG TGAGGGAACT 
GAGGCTGTAA AGAAATCTTT AGCGGGAGTA TTTGATGACA CTGTTCCAGA TGGCAAGAGG 
GAGAAAGAAA TGGTCTTACC TGCCGTCCAC CAGCACAATC CTGACTGTGA CATTTGGGTC 
CACGAGTATT TCACCCCATC CTGGTTCTGT CTACCGAACA ATCAGCCAGC CCTGACGGTC 
GTCCGGCCGG GGGACACGGC CCGGGATACC CTGGAGCTGA TCTGCAAGGC GCATAAACTG 
GATCATTCTG CTCATTACCT GCGCCTGAAA TTTCTAATAG AAAACAAAAT GCAGCATTAT 
GTGCCAAAGC CCGAGGAGGA CATTTATGAG CTGCTGTACA AAGAAATTGA AATTTGTCCA 
AAAGTCACTC AGTACATCCA GATTGAGAAG TCAGATACAG CTGGTGATAA TTATGGGTTT 
TCACTTTCTT CTGTGGAAGA GGATGGTGTT CGAAGGCTCT ACGTGAATAG TGTAAAAGAA 
ACTGGCTTAG CTTCCAAGAA AGGCCTGAAG GCGGGAGATG AGATTCTTGA GATCAATAAG 
CGGGCTGCAG GCACCCTGAA CTCCTCTGTG CTCAGAGATT TCCTCACACA GCCGTCCCTG 
GGCCTCCTGG TGAGGACCTG CCCCGAGCTG GAGGGAGGAG CAGAGCTGCT GGAGAACCCG 
CCCCATCGGC TGGACGGCCC GGCTGACCTC GGCGAGAGCC CCCTCGCCTT TCTCAGCAGT 
GACACAGGCC ACAGCCTCTG CAGCGAGCAG GGCAGCAGTG CTGAGGCTGC TCCGGAGGAG 
GTGGAAGGGC CGGACTTGGA ATCCTCAGAT GATACTGATC ACAGCAGCAA GAGCACGGAG 
CAGGTGGCCG CATTCTGCCG TAGTCTGCAC GAGATGAAGC CCTCTGACCC GAGCCCGTCC 
CCCCAGGACT GCACTGGGCC TCAGCTGGCC GCCCTGAGGC AGCTCACGGA TGCCGACAAG 
CTGCGCAAGG TCATCTGCGA GCTCCTGGAG ACAGAGCGCA CCTACGTGAA GGACTTAAAC 
TGTCTCATGG AGAGATACCT AAAACCTCTT CAAAAAGAAA CTTTTCTCAC CCAGGATGAG 
CTTGATGTGC TTTTTGGAAA TTTAACCGAA ATGGTAGAGT TTCAAGTAGA ATTCCTTAAA 
ACTCTGGAAG ATGGAGTGAG ATTGGTACCT GATTTGGAAA AGCTTGAGAA AGTTGATCAA 
TTTAAGAAAG TGCTGTTCTC CCTGGGGGGA TCCTTTCTGT ATTACGCTGA CCGCTTCAAG 
CTCTACAGTG CCTTCTGCGC CAGCCACACG AAGGTCCCCA AGGTCCTGGT GAAAGCCAAG 
ACGGACGCAG CTTTCAAGGC GTTCCTGGAC GCCCAGAACC CAAAGCAGCA GCACTCGTCC 
ACCCTGGAGT CTTACCTCAT CAAGCCCATC CAGAGGATCC TTAAGTATCC GCTTCTGCTG 
AAGGAGCTTT TTGCTCTGAC GGACGCGGAG AGTGAGGAAC ACTACCACCT GGATGTGGCC 
ATCAAGACTA TGAACAAGGT TGCAAGTCAT ATCAATGAAA TGCAGAAAAT CCATGAGGAA 
TTTGGGGCTG TGTTTGACCA GCTGATTGCT GAACAGACCG GTGAGAAAAA AGAGGTTGCG 
GATCTGAGCA TGGGAGACCT GCTGTTGCAT ACCACCGTCA TCTGGCCAAA CCCGCCCGCC 
TCACTGGGCA AGTGGAAAAA GGAGCCTGAA TTGGCAGCAT TTGTCTTCAA AACTGCCGTG 
GTCCTTGTCT ATAAAGATGG TACCAAACAG AAGAAGAAAC TTGTAGGATC ACACAGGCTT 
TCAATTTACG AGGACTGGGA CCCCTTCCGA TTTCGACACA TGATTCCTAC TGAAGCTCTG 
CAGGTTCGCG CTCTGGCAAG TGCAGATGCA GAAGCAAATG CCGTGTGTGA AATCGTCCAT 
GTAAAGTCGG AGTCGGAAGG GAGGCCGGAG AGGGTCTTCC ACCTGTGCTG CAGCTCCCCA 
GAGAGCCGAA AGGATTTCCT GAAGGCTGTG CATTCCATCC TGCGAGATAA GCACAGAAGA 
CAGCTCCTGA AGACTGAGAG CCTTCCCTCG TCCCAGCAAT ATGTCCCTTT CGGAGGAAAG 
CGGTTGTGTG CGCTGAAAGG GGCCAGGCCG GCTATGAGCA GGGCAGTGTC CGCCCCAAGC 
AAGTCTCTTG GGAGGAGGAG GCGGCGACTG GCCCGAAACA GGTTTACCAT TGATTCCGAT 
GCCATCTCCG CCAGCAGCCC GGAGAAAGAC CCCCAGCAGC CCCCTGGTGG TGGGGACACT 
GACCGCTGGG TGGAGGAACA GTTTGATCTT GCTCAGTATG AAGAGCAGGA TGACATCAAG 
GAGACAGACA TCCTCAGTGA CGATGATGAG TTCTGTGAGT CGGCCAAGGG CGCCTCGGCG 
GACAGAGACC TGCAGGAGCG GCTTCAGGCT GCCTCCATCA CTCAGCGGGA GAAAGGCCGG 
AAGCCCCTGG ACAGCCACGC GTCCCGCATG ACGCAGCTCA AGAAGCAAGC CGCCCTGTCG 
GGCATCAACG GAGGCCCGGA GAGTACGAGC GAGGAGGTCA TTTGGGTTAG GCGCGAAGAC 
TTTGCCCCCT CCCGCAAGCT GAACACTGAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIAM1 ( XM_006053559.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053621.1 Bubalus bubalis T-cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X2, mRNA.

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CloneID	OBu11127
Clone ID Related Accession (Same CDS sequence)	XM_006053558.1 , XM_006053560.1 , XM_006053559.1
Accession Version	XM_006053559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	T-lymphoma invasion and metastasis-inducing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784534.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACG CAGAAAGTCA AAATGTCGTG CATGAGTTCT ATGGAGACAA GCACGCCGTC 
CTGGGGCGCA AGCACACGTC GCGATCCCTG CGCCTGTCGC ACAAGGCGCG GCGGACCAGG 
CACGCCTCCT CCGGCAAGGT GATCCACAGG AACTCCGAAG TGAGTACCCG GTCCAGCAGC 
ACCCCCAGCA TCCCCCAGTC CCTGGCTGAA AATAGCCTGG AGCCCTTCTC CCAGGAAGGC 
ACCCTCGAGG ACTTCGGGAG CCCCATTTGG GTGGACCGAG TGGATATGGG CTTGAGACCC 
GTGTCTTACA CCGATTCCTC CGTCGCTCCC AGCGTGGACA GCAGCATCGT CCTCACAGCA 
GCCTCCGTGC AGAGCATGCC AGACTCGGAA GACAGCCGGC TTTATGGGGA TGACGCGACG 
TACTTGGCGG AGGGCGGCAG GCGGCAGCGC CCCTATGCCT CCAACGGACC CGCGTTCATG 
GAGACGGCGA GCTTTAAGAA GAAACGCTCC AAATCCGCAG ACATCTGGCG GGAGGACAGC 
CTGGAATTCT CACTGTCTGA TCTGAGCCAA GAACATTTAA CAAGCAACGA AGAGATCTTG 
GGTTCCGCCG AGGAGAAAGA CTGCGAGGAG GCTCGGGCGA TGGAAACCCG GGCGAGTCCG 
CGGCAGCTCA GTGCCTGTCA ACGGGCCAAT TCTCTGGGGG ACTTGTACGC TCAGAAGAAC 
CCTGGGGTGA CGGCAAACGG GGGGCCCAGG AGCAAACTTG CAGGCTACTG TCGGAATTTG 
GTGTCTGACA TCCCTGATCT CGCAAACCAT AAGATGCCAC CAGCTGCCGC TGAAGAGGCT 
CTCCCCTACA GTAATTATAA CACACTCCCC TGTAGGAAAT CTCACTGTCT CTCTGAAGGT 
GCCACCAACC CACAGATTAG CCATAGCAGC AGCATGCAAG GCAGAAGAGC AAAAACCACT 
CAGGATGTTA ACACAGGCGA GGGTAGCGAG TTTGCTGACA GCGGGATCGA AGGGGCCACC 
ACCGACACGG ACCTCCTGTC CCGGCGATCC AATGCCACTA ACTCCAGCTA CTCGCCCCCC 
ACCGGCCGTG CCTTTGTGGG CAGCGACAGC GGCAGCAGCT CCACCGGGGA CGCGGCCCGC 
CAGGGGGTGT ATGAGAATTT TCGCCGGGAG CTGGAGATGA GCACCACGAA CAGCGAGAGC 
CTGGAGGAGG CGGGCTCCGC GCACAGCGAC GAGCAGAGCA GCGGCACCCT GAGCTCCCCG 
GGCCAGTCCG ACATCCTGCT CACGGCCGCC CAGGGCACAG TGCGCAAGGC CGGCGCCCTG 
GCCGTCAAGA ACTTCCTGGT GCACAAGAAG AACAAGAAGG TGGAGTCAGC CACGCGGAGG 
AAGTGGAAGC ACTACTGGGT GTCCTTGAAA GGGTGCACAC TCTTCTTCTA CGAGGGCGAC 
GGCAGGTCTG GGATAGACCA CAACAGCGTC CCCAAGCACG CCGTCTGGGT GGAGAACAGC 
ATTGTACAGG CAGTTCCGGA GCACCCCAAG AAGGACTTCG TCTTCTGCCT CAGCAATTCC 
CTGGGCGATG CCTTCCTCTT CCAGACCACC AGCCAGACGG AGCTGGAGAA CTGGATCACC 
GCCATCCACT CTGCTTGCGC GGCCGCTGTC GCAAGACACC ACCACAAAGA GGACACGCTC 
CGGCTGCTGA AGTCGGAGAT CAAAAAACTG GAACAAAAGA TTGACATGGA CGAGAAGATG 
AAGAAAATGG GCGAAATGCA GCTGTCTTCC GTCACGGATT CAAAGAAGAA GAAAACTATA 
TTAGATCAGA TCTTTGTCTG GGAGCAGAAT CTTGAGCAGT TCCAAATGGA CCTGTTTCGG 
TACCGCTGTT ACCTAGCCAG CCTCCAAGGC GGAGAGCTGC CCAACCCTAA AAGGTTACTT 
GCCTTTGCAA GCAGACCAAC CAAAGTGGCA ATGGGCCGCC TTGGAATTTT TTCCGTGTCA 
TCTTTTCATG CCCTGGTGGC TGCCCGCACT GGTGAAACTG GAGTCAGAAG ACGTACTCAG 
GCCATGTCCC GATCTGCAAG CAAGAGAAGG AGCAGGTTTT CTTCTCTTTG GGGTCTGGAC 
ACTACCTCTA AAAAGAAGCA GGGACGCCCA AGCATCAATC AGGTGTTCGG TGAGGGAACT 
GAGGCTGTAA AGAAATCTTT AGCGGGAGTA TTTGATGACA CTGTTCCAGA TGGCAAGAGG 
GAGAAAGAAA TGGTCTTACC TGCCGTCCAC CAGCACAATC CTGACTGTGA CATTTGGGTC 
CACGAGTATT TCACCCCATC CTGGTTCTGT CTACCGAACA ATCAGCCAGC CCTGACGGTC 
GTCCGGCCGG GGGACACGGC CCGGGATACC CTGGAGCTGA TCTGCAAGGC GCATAAACTG 
GATCATTCTG CTCATTACCT GCGCCTGAAA TTTCTAATAG AAAACAAAAT GCAGCATTAT 
GTGCCAAAGC CCGAGGAGGA CATTTATGAG CTGCTGTACA AAGAAATTGA AATTTGTCCA 
AAAGTCACTC AGTACATCCA GATTGAGAAG TCAGATACAG CTGGTGATAA TTATGGGTTT 
TCACTTTCTT CTGTGGAAGA GGATGGTGTT CGAAGGCTCT ACGTGAATAG TGTAAAAGAA 
ACTGGCTTAG CTTCCAAGAA AGGCCTGAAG GCGGGAGATG AGATTCTTGA GATCAATAAG 
CGGGCTGCAG GCACCCTGAA CTCCTCTGTG CTCAGAGATT TCCTCACACA GCCGTCCCTG 
GGCCTCCTGG TGAGGACCTG CCCCGAGCTG GAGGGAGGAG CAGAGCTGCT GGAGAACCCG 
CCCCATCGGC TGGACGGCCC GGCTGACCTC GGCGAGAGCC CCCTCGCCTT TCTCAGCAGT 
GACACAGGCC ACAGCCTCTG CAGCGAGCAG GGCAGCAGTG CTGAGGCTGC TCCGGAGGAG 
GTGGAAGGGC CGGACTTGGA ATCCTCAGAT GATACTGATC ACAGCAGCAA GAGCACGGAG 
CAGGTGGCCG CATTCTGCCG TAGTCTGCAC GAGATGAAGC CCTCTGACCC GAGCCCGTCC 
CCCCAGGACT GCACTGGGCC TCAGCTGGCC GCCCTGAGGC AGCTCACGGA TGCCGACAAG 
CTGCGCAAGG TCATCTGCGA GCTCCTGGAG ACAGAGCGCA CCTACGTGAA GGACTTAAAC 
TGTCTCATGG AGAGATACCT AAAACCTCTT CAAAAAGAAA CTTTTCTCAC CCAGGATGAG 
CTTGATGTGC TTTTTGGAAA TTTAACCGAA ATGGTAGAGT TTCAAGTAGA ATTCCTTAAA 
ACTCTGGAAG ATGGAGTGAG ATTGGTACCT GATTTGGAAA AGCTTGAGAA AGTTGATCAA 
TTTAAGAAAG TGCTGTTCTC CCTGGGGGGA TCCTTTCTGT ATTACGCTGA CCGCTTCAAG 
CTCTACAGTG CCTTCTGCGC CAGCCACACG AAGGTCCCCA AGGTCCTGGT GAAAGCCAAG 
ACGGACGCAG CTTTCAAGGC GTTCCTGGAC GCCCAGAACC CAAAGCAGCA GCACTCGTCC 
ACCCTGGAGT CTTACCTCAT CAAGCCCATC CAGAGGATCC TTAAGTATCC GCTTCTGCTG 
AAGGAGCTTT TTGCTCTGAC GGACGCGGAG AGTGAGGAAC ACTACCACCT GGATGTGGCC 
ATCAAGACTA TGAACAAGGT TGCAAGTCAT ATCAATGAAA TGCAGAAAAT CCATGAGGAA 
TTTGGGGCTG TGTTTGACCA GCTGATTGCT GAACAGACCG GTGAGAAAAA AGAGGTTGCG 
GATCTGAGCA TGGGAGACCT GCTGTTGCAT ACCACCGTCA TCTGGCCAAA CCCGCCCGCC 
TCACTGGGCA AGTGGAAAAA GGAGCCTGAA TTGGCAGCAT TTGTCTTCAA AACTGCCGTG 
GTCCTTGTCT ATAAAGATGG TACCAAACAG AAGAAGAAAC TTGTAGGATC ACACAGGCTT 
TCAATTTACG AGGACTGGGA CCCCTTCCGA TTTCGACACA TGATTCCTAC TGAAGCTCTG 
CAGGTTCGCG CTCTGGCAAG TGCAGATGCA GAAGCAAATG CCGTGTGTGA AATCGTCCAT 
GTAAAGTCGG AGTCGGAAGG GAGGCCGGAG AGGGTCTTCC ACCTGTGCTG CAGCTCCCCA 
GAGAGCCGAA AGGATTTCCT GAAGGCTGTG CATTCCATCC TGCGAGATAA GCACAGAAGA 
CAGCTCCTGA AGACTGAGAG CCTTCCCTCG TCCCAGCAAT ATGTCCCTTT CGGAGGAAAG 
CGGTTGTGTG CGCTGAAAGG GGCCAGGCCG GCTATGAGCA GGGCAGTGTC CGCCCCAAGC 
AAGTCTCTTG GGAGGAGGAG GCGGCGACTG GCCCGAAACA GGTTTACCAT TGATTCCGAT 
GCCATCTCCG CCAGCAGCCC GGAGAAAGAC CCCCAGCAGC CCCCTGGTGG TGGGGACACT 
GACCGCTGGG TGGAGGAACA GTTTGATCTT GCTCAGTATG AAGAGCAGGA TGACATCAAG 
GAGACAGACA TCCTCAGTGA CGATGATGAG TTCTGTGAGT CGGCCAAGGG CGCCTCGGCG 
GACAGAGACC TGCAGGAGCG GCTTCAGGCT GCCTCCATCA CTCAGCGGGA GAAAGGCCGG 
AAGCCCCTGG ACAGCCACGC GTCCCGCATG ACGCAGCTCA AGAAGCAAGC CGCCCTGTCG 
GGCATCAACG GAGGCCCGGA GAGTACGAGC GAGGAGGTCA TTTGGGTTAG GCGCGAAGAC 
TTTGCCCCCT CCCGCAAGCT GAACACTGAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053621.1
CDS	458..5233
Translation	MGNAESQNVVHEFYGDKHAVLGRKHTSRSLRLSHKARRTRHASSGKVIHRNSEVSTRSSSTPSIPQSLAENSLEPFSQEGTLEDFGSPIWVDRVDMGLRPVSYTDSSVAPSVDSSIVLTAASVQSMPDSEDSRLYGDDATYLAEGGRRQRPYASNGPAFMETASFKKKRSKSADIWREDSLEFSLSDLSQEHLTSNEEILGSAEEKDCEEARAMETRASPRQLSACQRANSLGDLYAQKNPGVTANGGPRSKLAGYCRNLVSDIPDLANHKMPPAAAEEALPYSNYNTLPCRKSHCLSEGATNPQISHSSSMQGRRAKTTQDVNTGEGSEFADSGIEGATTDTDLLSRRSNATNSSYSPPTGRAFVGSDSGSSSTGDAARQGVYENFRRELEMSTTNSESLEEAGSAHSDEQSSGTLSSPGQSDILLTAAQGTVRKAGALAVKNFLVHKKNKKVESATRRKWKHYWVSLKGCTLFFYEGDGRSGIDHNSVPKHAVWVENSIVQAVPEHPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACAAAVARHHHKEDTLRLLKSEIKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQIFVWEQNLEQFQMDLFRYRCYLASLQGGELPNPKRLLAFASRPTKVAMGRLGIFSVSSFHALVAARTGETGVRRRTQAMSRSASKRRSRFSSLWGLDTTSKKKQGRPSINQVFGEGTEAVKKSLAGVFDDTVPDGKREKEMVLPAVHQHNPDCDIWVHEYFTPSWFCLPNNQPALTVVRPGDTARDTLELICKAHKLDHSAHYLRLKFLIENKMQHYVPKPEEDIYELLYKEIEICPKVTQYIQIEKSDTAGDNYGFSLSSVEEDGVRRLYVNSVKETGLASKKGLKAGDEILEINKRAAGTLNSSVLRDFLTQPSLGLLVRTCPELEGGAELLENPPHRLDGPADLGESPLAFLSSDTGHSLCSEQGSSAEAAPEEVEGPDLESSDDTDHSSKSTEQVAAFCRSLHEMKPSDPSPSPQDCTGPQLAALRQLTDADKLRKVICELLETERTYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKTDAAFKAFLDAQNPKQQHSSTLESYLIKPIQRILKYPLLLKELFALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVADLSMGDLLLHTTVIWPNPPASLGKWKKEPELAAFVFKTAVVLVYKDGTKQKKKLVGSHRLSIYEDWDPFRFRHMIPTEALQVRALASADAEANAVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKAVHSILRDKHRRQLLKTESLPSSQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDAISASSPEKDPQQPPGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESAKGASADRDLQERLQAASITQREKGRKPLDSHASRMTQLKKQAALSGINGGPESTSEEVIWVRREDFAPSRKLNTEI

Target ORF information:

RefSeq Version	XM_006053559.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis T-cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053559.1

ORF Insert Sequence:

ATGGGAAACG CAGAAAGTCA AAATGTCGTG CATGAGTTCT ATGGAGACAA GCACGCCGTC 
CTGGGGCGCA AGCACACGTC GCGATCCCTG CGCCTGTCGC ACAAGGCGCG GCGGACCAGG 
CACGCCTCCT CCGGCAAGGT GATCCACAGG AACTCCGAAG TGAGTACCCG GTCCAGCAGC 
ACCCCCAGCA TCCCCCAGTC CCTGGCTGAA AATAGCCTGG AGCCCTTCTC CCAGGAAGGC 
ACCCTCGAGG ACTTCGGGAG CCCCATTTGG GTGGACCGAG TGGATATGGG CTTGAGACCC 
GTGTCTTACA CCGATTCCTC CGTCGCTCCC AGCGTGGACA GCAGCATCGT CCTCACAGCA 
GCCTCCGTGC AGAGCATGCC AGACTCGGAA GACAGCCGGC TTTATGGGGA TGACGCGACG 
TACTTGGCGG AGGGCGGCAG GCGGCAGCGC CCCTATGCCT CCAACGGACC CGCGTTCATG 
GAGACGGCGA GCTTTAAGAA GAAACGCTCC AAATCCGCAG ACATCTGGCG GGAGGACAGC 
CTGGAATTCT CACTGTCTGA TCTGAGCCAA GAACATTTAA CAAGCAACGA AGAGATCTTG 
GGTTCCGCCG AGGAGAAAGA CTGCGAGGAG GCTCGGGCGA TGGAAACCCG GGCGAGTCCG 
CGGCAGCTCA GTGCCTGTCA ACGGGCCAAT TCTCTGGGGG ACTTGTACGC TCAGAAGAAC 
CCTGGGGTGA CGGCAAACGG GGGGCCCAGG AGCAAACTTG CAGGCTACTG TCGGAATTTG 
GTGTCTGACA TCCCTGATCT CGCAAACCAT AAGATGCCAC CAGCTGCCGC TGAAGAGGCT 
CTCCCCTACA GTAATTATAA CACACTCCCC TGTAGGAAAT CTCACTGTCT CTCTGAAGGT 
GCCACCAACC CACAGATTAG CCATAGCAGC AGCATGCAAG GCAGAAGAGC AAAAACCACT 
CAGGATGTTA ACACAGGCGA GGGTAGCGAG TTTGCTGACA GCGGGATCGA AGGGGCCACC 
ACCGACACGG ACCTCCTGTC CCGGCGATCC AATGCCACTA ACTCCAGCTA CTCGCCCCCC 
ACCGGCCGTG CCTTTGTGGG CAGCGACAGC GGCAGCAGCT CCACCGGGGA CGCGGCCCGC 
CAGGGGGTGT ATGAGAATTT TCGCCGGGAG CTGGAGATGA GCACCACGAA CAGCGAGAGC 
CTGGAGGAGG CGGGCTCCGC GCACAGCGAC GAGCAGAGCA GCGGCACCCT GAGCTCCCCG 
GGCCAGTCCG ACATCCTGCT CACGGCCGCC CAGGGCACAG TGCGCAAGGC CGGCGCCCTG 
GCCGTCAAGA ACTTCCTGGT GCACAAGAAG AACAAGAAGG TGGAGTCAGC CACGCGGAGG 
AAGTGGAAGC ACTACTGGGT GTCCTTGAAA GGGTGCACAC TCTTCTTCTA CGAGGGCGAC 
GGCAGGTCTG GGATAGACCA CAACAGCGTC CCCAAGCACG CCGTCTGGGT GGAGAACAGC 
ATTGTACAGG CAGTTCCGGA GCACCCCAAG AAGGACTTCG TCTTCTGCCT CAGCAATTCC 
CTGGGCGATG CCTTCCTCTT CCAGACCACC AGCCAGACGG AGCTGGAGAA CTGGATCACC 
GCCATCCACT CTGCTTGCGC GGCCGCTGTC GCAAGACACC ACCACAAAGA GGACACGCTC 
CGGCTGCTGA AGTCGGAGAT CAAAAAACTG GAACAAAAGA TTGACATGGA CGAGAAGATG 
AAGAAAATGG GCGAAATGCA GCTGTCTTCC GTCACGGATT CAAAGAAGAA GAAAACTATA 
TTAGATCAGA TCTTTGTCTG GGAGCAGAAT CTTGAGCAGT TCCAAATGGA CCTGTTTCGG 
TACCGCTGTT ACCTAGCCAG CCTCCAAGGC GGAGAGCTGC CCAACCCTAA AAGGTTACTT 
GCCTTTGCAA GCAGACCAAC CAAAGTGGCA ATGGGCCGCC TTGGAATTTT TTCCGTGTCA 
TCTTTTCATG CCCTGGTGGC TGCCCGCACT GGTGAAACTG GAGTCAGAAG ACGTACTCAG 
GCCATGTCCC GATCTGCAAG CAAGAGAAGG AGCAGGTTTT CTTCTCTTTG GGGTCTGGAC 
ACTACCTCTA AAAAGAAGCA GGGACGCCCA AGCATCAATC AGGTGTTCGG TGAGGGAACT 
GAGGCTGTAA AGAAATCTTT AGCGGGAGTA TTTGATGACA CTGTTCCAGA TGGCAAGAGG 
GAGAAAGAAA TGGTCTTACC TGCCGTCCAC CAGCACAATC CTGACTGTGA CATTTGGGTC 
CACGAGTATT TCACCCCATC CTGGTTCTGT CTACCGAACA ATCAGCCAGC CCTGACGGTC 
GTCCGGCCGG GGGACACGGC CCGGGATACC CTGGAGCTGA TCTGCAAGGC GCATAAACTG 
GATCATTCTG CTCATTACCT GCGCCTGAAA TTTCTAATAG AAAACAAAAT GCAGCATTAT 
GTGCCAAAGC CCGAGGAGGA CATTTATGAG CTGCTGTACA AAGAAATTGA AATTTGTCCA 
AAAGTCACTC AGTACATCCA GATTGAGAAG TCAGATACAG CTGGTGATAA TTATGGGTTT 
TCACTTTCTT CTGTGGAAGA GGATGGTGTT CGAAGGCTCT ACGTGAATAG TGTAAAAGAA 
ACTGGCTTAG CTTCCAAGAA AGGCCTGAAG GCGGGAGATG AGATTCTTGA GATCAATAAG 
CGGGCTGCAG GCACCCTGAA CTCCTCTGTG CTCAGAGATT TCCTCACACA GCCGTCCCTG 
GGCCTCCTGG TGAGGACCTG CCCCGAGCTG GAGGGAGGAG CAGAGCTGCT GGAGAACCCG 
CCCCATCGGC TGGACGGCCC GGCTGACCTC GGCGAGAGCC CCCTCGCCTT TCTCAGCAGT 
GACACAGGCC ACAGCCTCTG CAGCGAGCAG GGCAGCAGTG CTGAGGCTGC TCCGGAGGAG 
GTGGAAGGGC CGGACTTGGA ATCCTCAGAT GATACTGATC ACAGCAGCAA GAGCACGGAG 
CAGGTGGCCG CATTCTGCCG TAGTCTGCAC GAGATGAAGC CCTCTGACCC GAGCCCGTCC 
CCCCAGGACT GCACTGGGCC TCAGCTGGCC GCCCTGAGGC AGCTCACGGA TGCCGACAAG 
CTGCGCAAGG TCATCTGCGA GCTCCTGGAG ACAGAGCGCA CCTACGTGAA GGACTTAAAC 
TGTCTCATGG AGAGATACCT AAAACCTCTT CAAAAAGAAA CTTTTCTCAC CCAGGATGAG 
CTTGATGTGC TTTTTGGAAA TTTAACCGAA ATGGTAGAGT TTCAAGTAGA ATTCCTTAAA 
ACTCTGGAAG ATGGAGTGAG ATTGGTACCT GATTTGGAAA AGCTTGAGAA AGTTGATCAA 
TTTAAGAAAG TGCTGTTCTC CCTGGGGGGA TCCTTTCTGT ATTACGCTGA CCGCTTCAAG 
CTCTACAGTG CCTTCTGCGC CAGCCACACG AAGGTCCCCA AGGTCCTGGT GAAAGCCAAG 
ACGGACGCAG CTTTCAAGGC GTTCCTGGAC GCCCAGAACC CAAAGCAGCA GCACTCGTCC 
ACCCTGGAGT CTTACCTCAT CAAGCCCATC CAGAGGATCC TTAAGTATCC GCTTCTGCTG 
AAGGAGCTTT TTGCTCTGAC GGACGCGGAG AGTGAGGAAC ACTACCACCT GGATGTGGCC 
ATCAAGACTA TGAACAAGGT TGCAAGTCAT ATCAATGAAA TGCAGAAAAT CCATGAGGAA 
TTTGGGGCTG TGTTTGACCA GCTGATTGCT GAACAGACCG GTGAGAAAAA AGAGGTTGCG 
GATCTGAGCA TGGGAGACCT GCTGTTGCAT ACCACCGTCA TCTGGCCAAA CCCGCCCGCC 
TCACTGGGCA AGTGGAAAAA GGAGCCTGAA TTGGCAGCAT TTGTCTTCAA AACTGCCGTG 
GTCCTTGTCT ATAAAGATGG TACCAAACAG AAGAAGAAAC TTGTAGGATC ACACAGGCTT 
TCAATTTACG AGGACTGGGA CCCCTTCCGA TTTCGACACA TGATTCCTAC TGAAGCTCTG 
CAGGTTCGCG CTCTGGCAAG TGCAGATGCA GAAGCAAATG CCGTGTGTGA AATCGTCCAT 
GTAAAGTCGG AGTCGGAAGG GAGGCCGGAG AGGGTCTTCC ACCTGTGCTG CAGCTCCCCA 
GAGAGCCGAA AGGATTTCCT GAAGGCTGTG CATTCCATCC TGCGAGATAA GCACAGAAGA 
CAGCTCCTGA AGACTGAGAG CCTTCCCTCG TCCCAGCAAT ATGTCCCTTT CGGAGGAAAG 
CGGTTGTGTG CGCTGAAAGG GGCCAGGCCG GCTATGAGCA GGGCAGTGTC CGCCCCAAGC 
AAGTCTCTTG GGAGGAGGAG GCGGCGACTG GCCCGAAACA GGTTTACCAT TGATTCCGAT 
GCCATCTCCG CCAGCAGCCC GGAGAAAGAC CCCCAGCAGC CCCCTGGTGG TGGGGACACT 
GACCGCTGGG TGGAGGAACA GTTTGATCTT GCTCAGTATG AAGAGCAGGA TGACATCAAG 
GAGACAGACA TCCTCAGTGA CGATGATGAG TTCTGTGAGT CGGCCAAGGG CGCCTCGGCG 
GACAGAGACC TGCAGGAGCG GCTTCAGGCT GCCTCCATCA CTCAGCGGGA GAAAGGCCGG 
AAGCCCCTGG ACAGCCACGC GTCCCGCATG ACGCAGCTCA AGAAGCAAGC CGCCCTGTCG 
GGCATCAACG GAGGCCCGGA GAGTACGAGC GAGGAGGTCA TTTGGGTTAG GCGCGAAGAC 
TTTGCCCCCT CCCGCAAGCT GAACACTGAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIAM1 ( XM_006053558.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053620.2 Bubalus bubalis T cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X1, mRNA.

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CloneID	OBu42524
Clone ID Related Accession (Same CDS sequence)	XM_006053558.2 , XM_006053559.2
Accession Version	XM_006053558.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	T-lymphoma invasion and metastasis-inducing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006053558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACG CAGAAAGTCA AAATGTCGTG CATGAGTTCT ATGGAGACAA GCACGCCGTC 
CTGGGGCGCA AGCACACGTC GCGATCCCTG CGCCTGTCGC ACAAGGCGCG GCGGACCAGG 
CACGCCTCCT CCGGCAAGGT GATCCACAGG AACTCCGAAG TGAGTACCCG GTCCAGCAGC 
ACCCCCAGCA TCCCCCAGTC CCTGGCTGAA AATGGCCTGG AGCCCTTCTC CCAGGAAGGC 
ACCCTCGAGG ACTTCGGGAG CCCCATTTGG GTGGACCGAG TGGATATGGG CTTGAGACCC 
GTGTCTTACA CCGATTCCTC CGTCGCTCCC AGCGTGGACA GCAGCATCGT CCTCACAGCA 
GCCTCCGTGC AGAGCATGCC AGACTCGGAA GACAGCCGGC TTTATGGGGA TGACGCGACG 
TACTTGGCGG AGGGCGGCAG GCGGCAGCGC CCCTATGCCT CCAACGGACC CGCGTTCATG 
GAGACGGCGA GCTTTAAGAA GAAACGCTCC AAATCCGCAG ACATCTGGCG GGAGGACAGC 
CTGGAATTCT CACTGTCTGA TCTGAGCCAA GAACATTTAA CAAGCAACGA AGAGATCTTG 
GGTTCCGCCG AGGAGAAAGA CTGCGAGGAG GCTCGGGCGA TGGAAACCCG GGCGAGTCCG 
CGGCAGCTCA GTGCCTGTCA ACGGGCCAAT TCTCTGGGGG ACTTGTACGC TCAGAAGAAC 
CCTGGGGTGA CGGCAAACGG GGGGCCCAGG AGCAAACTTG CAGGCTACTG TCGGAATTTG 
GTGTCTGACA TCCCTGATCT CGCAAACCAT AAGATGCCAC CAGCTGCCGC TGAAGAGGCT 
CTCCCCTACA GTAATTATAA CACACTCCCC TGTAGGAAAT CTCACTGTCT CTCTGAAGGT 
GCCACCAACC CACAGATTAG CCATAGCAGC AGCATGCAAG GCAGAAGAGC AAAAACCACT 
CAGGATGTTA ACACAGGCGA GGGTAGCGAG TTTGCTGACA GCGGGATCGA AGGGGCCACC 
ACCGACACGG ACCTCCTGTC CCGGCGATCC AATGCCACTA ACTCCAGCTA CTCGCCCCCC 
ACCGGCCGTG CCTTTGTGGG CAGCGACAGC GGCAGCAGCT CCACCGGGGA CGCGGCCCGC 
CAGGGGGTGT ATGAGAATTT TCGCCGGGAG CTGGAGATGA GCACCACGAA CAGCGAGAGC 
CTGGAGGAGG CGGGCTCCGC GCACAGCGAC GAGCAGAGCA GCGGCACCCT GAGCTCCCCG 
GGCCAGTCCG ACATCCTGCT CACGGCCGCC CAGGGCACAG TGCGCAAGGC CGGCGCCCTG 
GCCGTCAAGA ACTTCCTGGT GCACAAGAAG AACAAGAAGG TGGAGTCAGC CACGCGGAGG 
AAGTGGAAGC ACTACTGGGT GTCCTTGAAA GGGTGCACAC TCTTCTTCTA TGAGGGCGAC 
GGCAGGTCCG GGATAGACCA CAACAGCGTC CCCAAGCACG CCGTCTGGGT GGAGAACAGC 
ATTGTACAGG CAGTTCCGGA GCACCCCAAG AAGGACTTCG TCTTCTGCCT CAGCAATTCC 
CTGGGCGATG CCTTCCTCTT CCAGACCACC AGCCAGACGG AGCTGGAGAA CTGGATCACC 
GCCATCCACT CTGCTTGCGC GGCCGCTGTC GCAAGACACC ACCACAAAGA GGACACGCTC 
CGGCTGCTGA AGTCGGAGAT CAAAAAACTG GAACAAAAGA TTGACATGGA CGAGAAGATG 
AAGAAAATGG GCGAAATGCA GCTGTCTTCC GTCACGGATT CAAAGAAGAA GAAAACTATA 
TTAGATCAGA TCTTTGTCTG GGAGCAGAAT CTTGAGCAGT TCCAAATGGA CCTGTTTCGG 
TACCGCTGTT ACCTAGCCAG CCTCCAAGGC GGAGAGCTGC CCAACCCTAA AAGGTTACTT 
GCCTTTGCAA GCAGACCAAC CAAAGTGGCA ATGGGCCGCC TTGGAATTTT TTCCGTGTCA 
TCTTTTCATG CCCTGGTGGC TGCCCGCACT GGTGAAACTG GAGTCAGAAG ACGTACTCAG 
GCCATGTCCC GATCTGCAAG CAAGAGAAGG AGCAGGTTTT CTTCTCTTTG GGGTCTGGAC 
ACTACCTCTA AAAAGAAGCA GGGACGCCCA AGCATCAATC AGGTGTTCGG TGAGGGAACT 
GAGGCTGTAA AGAAATCTTT AGCGGGAGTA TTTGATGACA CTGTTCCAGA TGGCAAGAGG 
GAGAAAGAAA TGGTCTTACC TGCCGTCCAC CAGCACAATC CTGACTGTGA CATTTGGGTC 
CACGAGTATT TCACCCCATC CTGGTTCTGT CTACCGAACA ATCAGCCAGC CCTGACGGTC 
GTCCGGCCGG GGGACACGGC CCGGGATACC CTGGAGCTGA TCTGCAAGGC GCATAAACTG 
GATCATTCTG CTCATTACCT GCGCCTGAAA TTTCTAATAG AAAACAAAAT GCAGCATTAT 
GTGCCAAAGC CCGAGGAGGA CATTTATGAG CTGCTGTACA AAGAAATTGA AATTTGTCCA 
AAAGTCACTC AGAACATCCA GATTGAGAAG TCAGATACAG CTGGTGATAA TTATGGGTTT 
TCACTTTCTT CTGTGGAAGA GGATGGTGTT CGAAGGCTCT ACGTGAATAG TGTAAAAGAA 
ACTGGCTTAG CTTCCAAGAA AGGCCTGAAG GCGGGAGATG AGATTCTTGA GATCAATAAG 
CGGGCTGCAG GCACCCTGAA CTCCTCTGTG CTCAGAGATT TCCTCACACA GCCGTCCCTG 
GGCCTCCTGG TGAGGACCTG CCCCGAGCTG GAGGGAGGAG CAGAGCTGCT GGAGAACCCG 
CCCCATCGGC TGGACGGCCC GGCTGACCTC GGCGAGAGCC CCCTCGCCTT TCTCAGCAGT 
GACACAGGCC ACAGCCTCTG CAGCGAGCAG GGCAGCAGTG CTGAGGCTGC TCCGGAGGAG 
GTGGAAGGGC CGGACTTGGA ATCCTCAGAT GATACTGATC ACAGCAGCAA GAGCACGGAG 
CAGGTGGCCG CATTCTGCCG TAGTCTGCAC GAGATGAAGC CCTCTGACCC GAGCCCGTCC 
CCCCAGGACT GCACTGGGCC TCAGCTGGCC GCCCTGAGGC AGCTCACGGA TGCCGACAAG 
CTGCGCAAGG TCATCTGCGA GCTCCTGGAG ACAGAGCGCA CCTACGTGAA GGACTTAAAC 
TGTCTCATGG AGAGATACCT AAAACCTCTT CAAAAAGAAA CTTTTCTCAC CCAGGATGAG 
CTTGATGTGC TTTTTGGAAA TTTAACCGAA ATGGTAGAGT TTCAAGTAGA ATTCCTTAAA 
ACTCTGGAAG ATGGAGTGAG ATTGGTACCT GATTTGGAAA AGCTTGAGAA AGTTGATCAA 
TTTAAGAAAG TGCTGTTCTC CCTGGGGGGA TCCTTTCTGT ATTACGCTGA CCGCTTCAAG 
CTCTACAGTG CCTTCTGCGC CAGCCACACG AAGGTCCCCA AGGTCCTGGT GAAAGCCAAG 
ACGGACGCAG CTTTCAAGGC GTTCCTGGAC GCCCAGAACC CAAAGCAGCA GCACTCGTCC 
ACCCTGGAGT CTTACCTCAT CAAGCCCATC CAGAGGATCC TTAAGTATCC GCTTCTGCTG 
AAGGAGCTTT TTGCTCTGAC GGACGCGGAG AGTGAGGAAC ACTACCACCT GGATGTGGCC 
ATCAAGACTA TGAACAAGGT TGCAAGTCAT ATCAATGAAA TGCAGAAAAT CCATGAGGAA 
TTTGGGGCTG TGTTTGACCA GCTGATTGCT GAACAGACCG GTGAGAAAAA AGAGGTTGCG 
GATCTGAGCA TGGGAGACCT GCTGTTGCAT ACCACCGTCA TCTGGCCAAA CCCGCCCGCC 
TCACTGGGCA AGTGGAAAAA GGAGCCTGAA TTGGCAGCAT TTGTCTTCAA AACTGCCGTG 
GTCCTTGTCT ATAAAGATGG TACCAAACAG AAGAAGAAAC TTGTAGGATC ACACAGGCTT 
TCAATTTACG AGGACTGGGA CCCCTTCCGA TTTCGACACA TGATTCCTAC TGAAGCTCTG 
CAGGTTCGCG CTCTGGCAAG TGCAGATGCA GAAGCAAATG CCGTGTGTGA AATCGTCCAT 
GTAAAGTCGG AGTCGGAAGG GAGGCCGGAG AGGGTCTTCC ACCTGTGCTG CAGCTCCCCA 
GAGAGCCGAA AGGATTTCCT GAAGGCTGTG CATTCCATCC TGCGAGATAA GCACAGAAGA 
CAGCTCCTGA AGACTGAGAG CCTTCCCTCG TCCCAGCAAT ATGTCCCTTT CGGAGGAAAG 
CGGTTGTGTG CGCTGAAAGG GGCCAGGCCG GCTATGAGCA GGGCAGTGTC CGCCCCAAGC 
AAGTCTCTTG GGAGGAGGAG GCGGCGACTG GCCCGAAACA GGTTTACCAT TGATTCCGAT 
GCCATCTCCG CCAGCAGCCC GGAGAAAGAC CCCCAGCAGC CCCCTGGTGG TGGGGACACT 
GACCGCTGGG TGGAGGAACA GTTTGATCTT GCTCAGTATG AAGAGCAGGA TGACATCAAG 
GAGACAGACA TCCTCAGTGA CGATGATGAG TTCTGTGAGT CGGCCAAGGG CGCCTCGGCG 
GACAGAGACC TGCAGGAGCG GCTTCAGGCT GCCTCCATCA CTCAGCGGGA GAAAGGCCGG 
AAGCCCCTGG ACAGCCACGC GTCCCGCATG ACGCAGCTCA AGAAGCAAGC CGCCCTGTCG 
GGCATCAACG GAGGCCCGGA GAGTACGAGC GAGGAGGTCA TTTGGGTTAG GCGCGAAGAC 
TTTGCCCCCT CCCGCAAGCT GAACACTGAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053620.2
CDS	315..5090
Translation	MGNAESQNVVHEFYGDKHAVLGRKHTSRSLRLSHKARRTRHASSGKVIHRNSEVSTRSSSTPSIPQSLAENGLEPFSQEGTLEDFGSPIWVDRVDMGLRPVSYTDSSVAPSVDSSIVLTAASVQSMPDSEDSRLYGDDATYLAEGGRRQRPYASNGPAFMETASFKKKRSKSADIWREDSLEFSLSDLSQEHLTSNEEILGSAEEKDCEEARAMETRASPRQLSACQRANSLGDLYAQKNPGVTANGGPRSKLAGYCRNLVSDIPDLANHKMPPAAAEEALPYSNYNTLPCRKSHCLSEGATNPQISHSSSMQGRRAKTTQDVNTGEGSEFADSGIEGATTDTDLLSRRSNATNSSYSPPTGRAFVGSDSGSSSTGDAARQGVYENFRRELEMSTTNSESLEEAGSAHSDEQSSGTLSSPGQSDILLTAAQGTVRKAGALAVKNFLVHKKNKKVESATRRKWKHYWVSLKGCTLFFYEGDGRSGIDHNSVPKHAVWVENSIVQAVPEHPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACAAAVARHHHKEDTLRLLKSEIKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQIFVWEQNLEQFQMDLFRYRCYLASLQGGELPNPKRLLAFASRPTKVAMGRLGIFSVSSFHALVAARTGETGVRRRTQAMSRSASKRRSRFSSLWGLDTTSKKKQGRPSINQVFGEGTEAVKKSLAGVFDDTVPDGKREKEMVLPAVHQHNPDCDIWVHEYFTPSWFCLPNNQPALTVVRPGDTARDTLELICKAHKLDHSAHYLRLKFLIENKMQHYVPKPEEDIYELLYKEIEICPKVTQNIQIEKSDTAGDNYGFSLSSVEEDGVRRLYVNSVKETGLASKKGLKAGDEILEINKRAAGTLNSSVLRDFLTQPSLGLLVRTCPELEGGAELLENPPHRLDGPADLGESPLAFLSSDTGHSLCSEQGSSAEAAPEEVEGPDLESSDDTDHSSKSTEQVAAFCRSLHEMKPSDPSPSPQDCTGPQLAALRQLTDADKLRKVICELLETERTYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKTDAAFKAFLDAQNPKQQHSSTLESYLIKPIQRILKYPLLLKELFALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVADLSMGDLLLHTTVIWPNPPASLGKWKKEPELAAFVFKTAVVLVYKDGTKQKKKLVGSHRLSIYEDWDPFRFRHMIPTEALQVRALASADAEANAVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKAVHSILRDKHRRQLLKTESLPSSQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDAISASSPEKDPQQPPGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESAKGASADRDLQERLQAASITQREKGRKPLDSHASRMTQLKKQAALSGINGGPESTSEEVIWVRREDFAPSRKLNTEI

Target ORF information:

RefSeq Version	XM_006053558.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis T cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053558.2

ORF Insert Sequence:

ATGGGAAACG CAGAAAGTCA AAATGTCGTG CATGAGTTCT ATGGAGACAA GCACGCCGTC 
CTGGGGCGCA AGCACACGTC GCGATCCCTG CGCCTGTCGC ACAAGGCGCG GCGGACCAGG 
CACGCCTCCT CCGGCAAGGT GATCCACAGG AACTCCGAAG TGAGTACCCG GTCCAGCAGC 
ACCCCCAGCA TCCCCCAGTC CCTGGCTGAA AATGGCCTGG AGCCCTTCTC CCAGGAAGGC 
ACCCTCGAGG ACTTCGGGAG CCCCATTTGG GTGGACCGAG TGGATATGGG CTTGAGACCC 
GTGTCTTACA CCGATTCCTC CGTCGCTCCC AGCGTGGACA GCAGCATCGT CCTCACAGCA 
GCCTCCGTGC AGAGCATGCC AGACTCGGAA GACAGCCGGC TTTATGGGGA TGACGCGACG 
TACTTGGCGG AGGGCGGCAG GCGGCAGCGC CCCTATGCCT CCAACGGACC CGCGTTCATG 
GAGACGGCGA GCTTTAAGAA GAAACGCTCC AAATCCGCAG ACATCTGGCG GGAGGACAGC 
CTGGAATTCT CACTGTCTGA TCTGAGCCAA GAACATTTAA CAAGCAACGA AGAGATCTTG 
GGTTCCGCCG AGGAGAAAGA CTGCGAGGAG GCTCGGGCGA TGGAAACCCG GGCGAGTCCG 
CGGCAGCTCA GTGCCTGTCA ACGGGCCAAT TCTCTGGGGG ACTTGTACGC TCAGAAGAAC 
CCTGGGGTGA CGGCAAACGG GGGGCCCAGG AGCAAACTTG CAGGCTACTG TCGGAATTTG 
GTGTCTGACA TCCCTGATCT CGCAAACCAT AAGATGCCAC CAGCTGCCGC TGAAGAGGCT 
CTCCCCTACA GTAATTATAA CACACTCCCC TGTAGGAAAT CTCACTGTCT CTCTGAAGGT 
GCCACCAACC CACAGATTAG CCATAGCAGC AGCATGCAAG GCAGAAGAGC AAAAACCACT 
CAGGATGTTA ACACAGGCGA GGGTAGCGAG TTTGCTGACA GCGGGATCGA AGGGGCCACC 
ACCGACACGG ACCTCCTGTC CCGGCGATCC AATGCCACTA ACTCCAGCTA CTCGCCCCCC 
ACCGGCCGTG CCTTTGTGGG CAGCGACAGC GGCAGCAGCT CCACCGGGGA CGCGGCCCGC 
CAGGGGGTGT ATGAGAATTT TCGCCGGGAG CTGGAGATGA GCACCACGAA CAGCGAGAGC 
CTGGAGGAGG CGGGCTCCGC GCACAGCGAC GAGCAGAGCA GCGGCACCCT GAGCTCCCCG 
GGCCAGTCCG ACATCCTGCT CACGGCCGCC CAGGGCACAG TGCGCAAGGC CGGCGCCCTG 
GCCGTCAAGA ACTTCCTGGT GCACAAGAAG AACAAGAAGG TGGAGTCAGC CACGCGGAGG 
AAGTGGAAGC ACTACTGGGT GTCCTTGAAA GGGTGCACAC TCTTCTTCTA TGAGGGCGAC 
GGCAGGTCCG GGATAGACCA CAACAGCGTC CCCAAGCACG CCGTCTGGGT GGAGAACAGC 
ATTGTACAGG CAGTTCCGGA GCACCCCAAG AAGGACTTCG TCTTCTGCCT CAGCAATTCC 
CTGGGCGATG CCTTCCTCTT CCAGACCACC AGCCAGACGG AGCTGGAGAA CTGGATCACC 
GCCATCCACT CTGCTTGCGC GGCCGCTGTC GCAAGACACC ACCACAAAGA GGACACGCTC 
CGGCTGCTGA AGTCGGAGAT CAAAAAACTG GAACAAAAGA TTGACATGGA CGAGAAGATG 
AAGAAAATGG GCGAAATGCA GCTGTCTTCC GTCACGGATT CAAAGAAGAA GAAAACTATA 
TTAGATCAGA TCTTTGTCTG GGAGCAGAAT CTTGAGCAGT TCCAAATGGA CCTGTTTCGG 
TACCGCTGTT ACCTAGCCAG CCTCCAAGGC GGAGAGCTGC CCAACCCTAA AAGGTTACTT 
GCCTTTGCAA GCAGACCAAC CAAAGTGGCA ATGGGCCGCC TTGGAATTTT TTCCGTGTCA 
TCTTTTCATG CCCTGGTGGC TGCCCGCACT GGTGAAACTG GAGTCAGAAG ACGTACTCAG 
GCCATGTCCC GATCTGCAAG CAAGAGAAGG AGCAGGTTTT CTTCTCTTTG GGGTCTGGAC 
ACTACCTCTA AAAAGAAGCA GGGACGCCCA AGCATCAATC AGGTGTTCGG TGAGGGAACT 
GAGGCTGTAA AGAAATCTTT AGCGGGAGTA TTTGATGACA CTGTTCCAGA TGGCAAGAGG 
GAGAAAGAAA TGGTCTTACC TGCCGTCCAC CAGCACAATC CTGACTGTGA CATTTGGGTC 
CACGAGTATT TCACCCCATC CTGGTTCTGT CTACCGAACA ATCAGCCAGC CCTGACGGTC 
GTCCGGCCGG GGGACACGGC CCGGGATACC CTGGAGCTGA TCTGCAAGGC GCATAAACTG 
GATCATTCTG CTCATTACCT GCGCCTGAAA TTTCTAATAG AAAACAAAAT GCAGCATTAT 
GTGCCAAAGC CCGAGGAGGA CATTTATGAG CTGCTGTACA AAGAAATTGA AATTTGTCCA 
AAAGTCACTC AGAACATCCA GATTGAGAAG TCAGATACAG CTGGTGATAA TTATGGGTTT 
TCACTTTCTT CTGTGGAAGA GGATGGTGTT CGAAGGCTCT ACGTGAATAG TGTAAAAGAA 
ACTGGCTTAG CTTCCAAGAA AGGCCTGAAG GCGGGAGATG AGATTCTTGA GATCAATAAG 
CGGGCTGCAG GCACCCTGAA CTCCTCTGTG CTCAGAGATT TCCTCACACA GCCGTCCCTG 
GGCCTCCTGG TGAGGACCTG CCCCGAGCTG GAGGGAGGAG CAGAGCTGCT GGAGAACCCG 
CCCCATCGGC TGGACGGCCC GGCTGACCTC GGCGAGAGCC CCCTCGCCTT TCTCAGCAGT 
GACACAGGCC ACAGCCTCTG CAGCGAGCAG GGCAGCAGTG CTGAGGCTGC TCCGGAGGAG 
GTGGAAGGGC CGGACTTGGA ATCCTCAGAT GATACTGATC ACAGCAGCAA GAGCACGGAG 
CAGGTGGCCG CATTCTGCCG TAGTCTGCAC GAGATGAAGC CCTCTGACCC GAGCCCGTCC 
CCCCAGGACT GCACTGGGCC TCAGCTGGCC GCCCTGAGGC AGCTCACGGA TGCCGACAAG 
CTGCGCAAGG TCATCTGCGA GCTCCTGGAG ACAGAGCGCA CCTACGTGAA GGACTTAAAC 
TGTCTCATGG AGAGATACCT AAAACCTCTT CAAAAAGAAA CTTTTCTCAC CCAGGATGAG 
CTTGATGTGC TTTTTGGAAA TTTAACCGAA ATGGTAGAGT TTCAAGTAGA ATTCCTTAAA 
ACTCTGGAAG ATGGAGTGAG ATTGGTACCT GATTTGGAAA AGCTTGAGAA AGTTGATCAA 
TTTAAGAAAG TGCTGTTCTC CCTGGGGGGA TCCTTTCTGT ATTACGCTGA CCGCTTCAAG 
CTCTACAGTG CCTTCTGCGC CAGCCACACG AAGGTCCCCA AGGTCCTGGT GAAAGCCAAG 
ACGGACGCAG CTTTCAAGGC GTTCCTGGAC GCCCAGAACC CAAAGCAGCA GCACTCGTCC 
ACCCTGGAGT CTTACCTCAT CAAGCCCATC CAGAGGATCC TTAAGTATCC GCTTCTGCTG 
AAGGAGCTTT TTGCTCTGAC GGACGCGGAG AGTGAGGAAC ACTACCACCT GGATGTGGCC 
ATCAAGACTA TGAACAAGGT TGCAAGTCAT ATCAATGAAA TGCAGAAAAT CCATGAGGAA 
TTTGGGGCTG TGTTTGACCA GCTGATTGCT GAACAGACCG GTGAGAAAAA AGAGGTTGCG 
GATCTGAGCA TGGGAGACCT GCTGTTGCAT ACCACCGTCA TCTGGCCAAA CCCGCCCGCC 
TCACTGGGCA AGTGGAAAAA GGAGCCTGAA TTGGCAGCAT TTGTCTTCAA AACTGCCGTG 
GTCCTTGTCT ATAAAGATGG TACCAAACAG AAGAAGAAAC TTGTAGGATC ACACAGGCTT 
TCAATTTACG AGGACTGGGA CCCCTTCCGA TTTCGACACA TGATTCCTAC TGAAGCTCTG 
CAGGTTCGCG CTCTGGCAAG TGCAGATGCA GAAGCAAATG CCGTGTGTGA AATCGTCCAT 
GTAAAGTCGG AGTCGGAAGG GAGGCCGGAG AGGGTCTTCC ACCTGTGCTG CAGCTCCCCA 
GAGAGCCGAA AGGATTTCCT GAAGGCTGTG CATTCCATCC TGCGAGATAA GCACAGAAGA 
CAGCTCCTGA AGACTGAGAG CCTTCCCTCG TCCCAGCAAT ATGTCCCTTT CGGAGGAAAG 
CGGTTGTGTG CGCTGAAAGG GGCCAGGCCG GCTATGAGCA GGGCAGTGTC CGCCCCAAGC 
AAGTCTCTTG GGAGGAGGAG GCGGCGACTG GCCCGAAACA GGTTTACCAT TGATTCCGAT 
GCCATCTCCG CCAGCAGCCC GGAGAAAGAC CCCCAGCAGC CCCCTGGTGG TGGGGACACT 
GACCGCTGGG TGGAGGAACA GTTTGATCTT GCTCAGTATG AAGAGCAGGA TGACATCAAG 
GAGACAGACA TCCTCAGTGA CGATGATGAG TTCTGTGAGT CGGCCAAGGG CGCCTCGGCG 
GACAGAGACC TGCAGGAGCG GCTTCAGGCT GCCTCCATCA CTCAGCGGGA GAAAGGCCGG 
AAGCCCCTGG ACAGCCACGC GTCCCGCATG ACGCAGCTCA AGAAGCAAGC CGCCCTGTCG 
GGCATCAACG GAGGCCCGGA GAGTACGAGC GAGGAGGTCA TTTGGGTTAG GCGCGAAGAC 
TTTGCCCCCT CCCGCAAGCT GAACACTGAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIAM1 ( XM_006053559.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053621.2 Bubalus bubalis T cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X2, mRNA.

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CloneID	OBu42524
Clone ID Related Accession (Same CDS sequence)	XM_006053558.2 , XM_006053559.2
Accession Version	XM_006053559.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	T-lymphoma invasion and metastasis-inducing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006053559.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACG CAGAAAGTCA AAATGTCGTG CATGAGTTCT ATGGAGACAA GCACGCCGTC 
CTGGGGCGCA AGCACACGTC GCGATCCCTG CGCCTGTCGC ACAAGGCGCG GCGGACCAGG 
CACGCCTCCT CCGGCAAGGT GATCCACAGG AACTCCGAAG TGAGTACCCG GTCCAGCAGC 
ACCCCCAGCA TCCCCCAGTC CCTGGCTGAA AATGGCCTGG AGCCCTTCTC CCAGGAAGGC 
ACCCTCGAGG ACTTCGGGAG CCCCATTTGG GTGGACCGAG TGGATATGGG CTTGAGACCC 
GTGTCTTACA CCGATTCCTC CGTCGCTCCC AGCGTGGACA GCAGCATCGT CCTCACAGCA 
GCCTCCGTGC AGAGCATGCC AGACTCGGAA GACAGCCGGC TTTATGGGGA TGACGCGACG 
TACTTGGCGG AGGGCGGCAG GCGGCAGCGC CCCTATGCCT CCAACGGACC CGCGTTCATG 
GAGACGGCGA GCTTTAAGAA GAAACGCTCC AAATCCGCAG ACATCTGGCG GGAGGACAGC 
CTGGAATTCT CACTGTCTGA TCTGAGCCAA GAACATTTAA CAAGCAACGA AGAGATCTTG 
GGTTCCGCCG AGGAGAAAGA CTGCGAGGAG GCTCGGGCGA TGGAAACCCG GGCGAGTCCG 
CGGCAGCTCA GTGCCTGTCA ACGGGCCAAT TCTCTGGGGG ACTTGTACGC TCAGAAGAAC 
CCTGGGGTGA CGGCAAACGG GGGGCCCAGG AGCAAACTTG CAGGCTACTG TCGGAATTTG 
GTGTCTGACA TCCCTGATCT CGCAAACCAT AAGATGCCAC CAGCTGCCGC TGAAGAGGCT 
CTCCCCTACA GTAATTATAA CACACTCCCC TGTAGGAAAT CTCACTGTCT CTCTGAAGGT 
GCCACCAACC CACAGATTAG CCATAGCAGC AGCATGCAAG GCAGAAGAGC AAAAACCACT 
CAGGATGTTA ACACAGGCGA GGGTAGCGAG TTTGCTGACA GCGGGATCGA AGGGGCCACC 
ACCGACACGG ACCTCCTGTC CCGGCGATCC AATGCCACTA ACTCCAGCTA CTCGCCCCCC 
ACCGGCCGTG CCTTTGTGGG CAGCGACAGC GGCAGCAGCT CCACCGGGGA CGCGGCCCGC 
CAGGGGGTGT ATGAGAATTT TCGCCGGGAG CTGGAGATGA GCACCACGAA CAGCGAGAGC 
CTGGAGGAGG CGGGCTCCGC GCACAGCGAC GAGCAGAGCA GCGGCACCCT GAGCTCCCCG 
GGCCAGTCCG ACATCCTGCT CACGGCCGCC CAGGGCACAG TGCGCAAGGC CGGCGCCCTG 
GCCGTCAAGA ACTTCCTGGT GCACAAGAAG AACAAGAAGG TGGAGTCAGC CACGCGGAGG 
AAGTGGAAGC ACTACTGGGT GTCCTTGAAA GGGTGCACAC TCTTCTTCTA TGAGGGCGAC 
GGCAGGTCCG GGATAGACCA CAACAGCGTC CCCAAGCACG CCGTCTGGGT GGAGAACAGC 
ATTGTACAGG CAGTTCCGGA GCACCCCAAG AAGGACTTCG TCTTCTGCCT CAGCAATTCC 
CTGGGCGATG CCTTCCTCTT CCAGACCACC AGCCAGACGG AGCTGGAGAA CTGGATCACC 
GCCATCCACT CTGCTTGCGC GGCCGCTGTC GCAAGACACC ACCACAAAGA GGACACGCTC 
CGGCTGCTGA AGTCGGAGAT CAAAAAACTG GAACAAAAGA TTGACATGGA CGAGAAGATG 
AAGAAAATGG GCGAAATGCA GCTGTCTTCC GTCACGGATT CAAAGAAGAA GAAAACTATA 
TTAGATCAGA TCTTTGTCTG GGAGCAGAAT CTTGAGCAGT TCCAAATGGA CCTGTTTCGG 
TACCGCTGTT ACCTAGCCAG CCTCCAAGGC GGAGAGCTGC CCAACCCTAA AAGGTTACTT 
GCCTTTGCAA GCAGACCAAC CAAAGTGGCA ATGGGCCGCC TTGGAATTTT TTCCGTGTCA 
TCTTTTCATG CCCTGGTGGC TGCCCGCACT GGTGAAACTG GAGTCAGAAG ACGTACTCAG 
GCCATGTCCC GATCTGCAAG CAAGAGAAGG AGCAGGTTTT CTTCTCTTTG GGGTCTGGAC 
ACTACCTCTA AAAAGAAGCA GGGACGCCCA AGCATCAATC AGGTGTTCGG TGAGGGAACT 
GAGGCTGTAA AGAAATCTTT AGCGGGAGTA TTTGATGACA CTGTTCCAGA TGGCAAGAGG 
GAGAAAGAAA TGGTCTTACC TGCCGTCCAC CAGCACAATC CTGACTGTGA CATTTGGGTC 
CACGAGTATT TCACCCCATC CTGGTTCTGT CTACCGAACA ATCAGCCAGC CCTGACGGTC 
GTCCGGCCGG GGGACACGGC CCGGGATACC CTGGAGCTGA TCTGCAAGGC GCATAAACTG 
GATCATTCTG CTCATTACCT GCGCCTGAAA TTTCTAATAG AAAACAAAAT GCAGCATTAT 
GTGCCAAAGC CCGAGGAGGA CATTTATGAG CTGCTGTACA AAGAAATTGA AATTTGTCCA 
AAAGTCACTC AGAACATCCA GATTGAGAAG TCAGATACAG CTGGTGATAA TTATGGGTTT 
TCACTTTCTT CTGTGGAAGA GGATGGTGTT CGAAGGCTCT ACGTGAATAG TGTAAAAGAA 
ACTGGCTTAG CTTCCAAGAA AGGCCTGAAG GCGGGAGATG AGATTCTTGA GATCAATAAG 
CGGGCTGCAG GCACCCTGAA CTCCTCTGTG CTCAGAGATT TCCTCACACA GCCGTCCCTG 
GGCCTCCTGG TGAGGACCTG CCCCGAGCTG GAGGGAGGAG CAGAGCTGCT GGAGAACCCG 
CCCCATCGGC TGGACGGCCC GGCTGACCTC GGCGAGAGCC CCCTCGCCTT TCTCAGCAGT 
GACACAGGCC ACAGCCTCTG CAGCGAGCAG GGCAGCAGTG CTGAGGCTGC TCCGGAGGAG 
GTGGAAGGGC CGGACTTGGA ATCCTCAGAT GATACTGATC ACAGCAGCAA GAGCACGGAG 
CAGGTGGCCG CATTCTGCCG TAGTCTGCAC GAGATGAAGC CCTCTGACCC GAGCCCGTCC 
CCCCAGGACT GCACTGGGCC TCAGCTGGCC GCCCTGAGGC AGCTCACGGA TGCCGACAAG 
CTGCGCAAGG TCATCTGCGA GCTCCTGGAG ACAGAGCGCA CCTACGTGAA GGACTTAAAC 
TGTCTCATGG AGAGATACCT AAAACCTCTT CAAAAAGAAA CTTTTCTCAC CCAGGATGAG 
CTTGATGTGC TTTTTGGAAA TTTAACCGAA ATGGTAGAGT TTCAAGTAGA ATTCCTTAAA 
ACTCTGGAAG ATGGAGTGAG ATTGGTACCT GATTTGGAAA AGCTTGAGAA AGTTGATCAA 
TTTAAGAAAG TGCTGTTCTC CCTGGGGGGA TCCTTTCTGT ATTACGCTGA CCGCTTCAAG 
CTCTACAGTG CCTTCTGCGC CAGCCACACG AAGGTCCCCA AGGTCCTGGT GAAAGCCAAG 
ACGGACGCAG CTTTCAAGGC GTTCCTGGAC GCCCAGAACC CAAAGCAGCA GCACTCGTCC 
ACCCTGGAGT CTTACCTCAT CAAGCCCATC CAGAGGATCC TTAAGTATCC GCTTCTGCTG 
AAGGAGCTTT TTGCTCTGAC GGACGCGGAG AGTGAGGAAC ACTACCACCT GGATGTGGCC 
ATCAAGACTA TGAACAAGGT TGCAAGTCAT ATCAATGAAA TGCAGAAAAT CCATGAGGAA 
TTTGGGGCTG TGTTTGACCA GCTGATTGCT GAACAGACCG GTGAGAAAAA AGAGGTTGCG 
GATCTGAGCA TGGGAGACCT GCTGTTGCAT ACCACCGTCA TCTGGCCAAA CCCGCCCGCC 
TCACTGGGCA AGTGGAAAAA GGAGCCTGAA TTGGCAGCAT TTGTCTTCAA AACTGCCGTG 
GTCCTTGTCT ATAAAGATGG TACCAAACAG AAGAAGAAAC TTGTAGGATC ACACAGGCTT 
TCAATTTACG AGGACTGGGA CCCCTTCCGA TTTCGACACA TGATTCCTAC TGAAGCTCTG 
CAGGTTCGCG CTCTGGCAAG TGCAGATGCA GAAGCAAATG CCGTGTGTGA AATCGTCCAT 
GTAAAGTCGG AGTCGGAAGG GAGGCCGGAG AGGGTCTTCC ACCTGTGCTG CAGCTCCCCA 
GAGAGCCGAA AGGATTTCCT GAAGGCTGTG CATTCCATCC TGCGAGATAA GCACAGAAGA 
CAGCTCCTGA AGACTGAGAG CCTTCCCTCG TCCCAGCAAT ATGTCCCTTT CGGAGGAAAG 
CGGTTGTGTG CGCTGAAAGG GGCCAGGCCG GCTATGAGCA GGGCAGTGTC CGCCCCAAGC 
AAGTCTCTTG GGAGGAGGAG GCGGCGACTG GCCCGAAACA GGTTTACCAT TGATTCCGAT 
GCCATCTCCG CCAGCAGCCC GGAGAAAGAC CCCCAGCAGC CCCCTGGTGG TGGGGACACT 
GACCGCTGGG TGGAGGAACA GTTTGATCTT GCTCAGTATG AAGAGCAGGA TGACATCAAG 
GAGACAGACA TCCTCAGTGA CGATGATGAG TTCTGTGAGT CGGCCAAGGG CGCCTCGGCG 
GACAGAGACC TGCAGGAGCG GCTTCAGGCT GCCTCCATCA CTCAGCGGGA GAAAGGCCGG 
AAGCCCCTGG ACAGCCACGC GTCCCGCATG ACGCAGCTCA AGAAGCAAGC CGCCCTGTCG 
GGCATCAACG GAGGCCCGGA GAGTACGAGC GAGGAGGTCA TTTGGGTTAG GCGCGAAGAC 
TTTGCCCCCT CCCGCAAGCT GAACACTGAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053621.2
CDS	459..5234
Translation	MGNAESQNVVHEFYGDKHAVLGRKHTSRSLRLSHKARRTRHASSGKVIHRNSEVSTRSSSTPSIPQSLAENGLEPFSQEGTLEDFGSPIWVDRVDMGLRPVSYTDSSVAPSVDSSIVLTAASVQSMPDSEDSRLYGDDATYLAEGGRRQRPYASNGPAFMETASFKKKRSKSADIWREDSLEFSLSDLSQEHLTSNEEILGSAEEKDCEEARAMETRASPRQLSACQRANSLGDLYAQKNPGVTANGGPRSKLAGYCRNLVSDIPDLANHKMPPAAAEEALPYSNYNTLPCRKSHCLSEGATNPQISHSSSMQGRRAKTTQDVNTGEGSEFADSGIEGATTDTDLLSRRSNATNSSYSPPTGRAFVGSDSGSSSTGDAARQGVYENFRRELEMSTTNSESLEEAGSAHSDEQSSGTLSSPGQSDILLTAAQGTVRKAGALAVKNFLVHKKNKKVESATRRKWKHYWVSLKGCTLFFYEGDGRSGIDHNSVPKHAVWVENSIVQAVPEHPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACAAAVARHHHKEDTLRLLKSEIKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQIFVWEQNLEQFQMDLFRYRCYLASLQGGELPNPKRLLAFASRPTKVAMGRLGIFSVSSFHALVAARTGETGVRRRTQAMSRSASKRRSRFSSLWGLDTTSKKKQGRPSINQVFGEGTEAVKKSLAGVFDDTVPDGKREKEMVLPAVHQHNPDCDIWVHEYFTPSWFCLPNNQPALTVVRPGDTARDTLELICKAHKLDHSAHYLRLKFLIENKMQHYVPKPEEDIYELLYKEIEICPKVTQNIQIEKSDTAGDNYGFSLSSVEEDGVRRLYVNSVKETGLASKKGLKAGDEILEINKRAAGTLNSSVLRDFLTQPSLGLLVRTCPELEGGAELLENPPHRLDGPADLGESPLAFLSSDTGHSLCSEQGSSAEAAPEEVEGPDLESSDDTDHSSKSTEQVAAFCRSLHEMKPSDPSPSPQDCTGPQLAALRQLTDADKLRKVICELLETERTYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKTDAAFKAFLDAQNPKQQHSSTLESYLIKPIQRILKYPLLLKELFALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVADLSMGDLLLHTTVIWPNPPASLGKWKKEPELAAFVFKTAVVLVYKDGTKQKKKLVGSHRLSIYEDWDPFRFRHMIPTEALQVRALASADAEANAVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKAVHSILRDKHRRQLLKTESLPSSQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDAISASSPEKDPQQPPGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESAKGASADRDLQERLQAASITQREKGRKPLDSHASRMTQLKKQAALSGINGGPESTSEEVIWVRREDFAPSRKLNTEI

Target ORF information:

RefSeq Version	XM_006053559.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis T cell lymphoma invasion and metastasis 1 (TIAM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053559.2

ORF Insert Sequence:

ATGGGAAACG CAGAAAGTCA AAATGTCGTG CATGAGTTCT ATGGAGACAA GCACGCCGTC 
CTGGGGCGCA AGCACACGTC GCGATCCCTG CGCCTGTCGC ACAAGGCGCG GCGGACCAGG 
CACGCCTCCT CCGGCAAGGT GATCCACAGG AACTCCGAAG TGAGTACCCG GTCCAGCAGC 
ACCCCCAGCA TCCCCCAGTC CCTGGCTGAA AATGGCCTGG AGCCCTTCTC CCAGGAAGGC 
ACCCTCGAGG ACTTCGGGAG CCCCATTTGG GTGGACCGAG TGGATATGGG CTTGAGACCC 
GTGTCTTACA CCGATTCCTC CGTCGCTCCC AGCGTGGACA GCAGCATCGT CCTCACAGCA 
GCCTCCGTGC AGAGCATGCC AGACTCGGAA GACAGCCGGC TTTATGGGGA TGACGCGACG 
TACTTGGCGG AGGGCGGCAG GCGGCAGCGC CCCTATGCCT CCAACGGACC CGCGTTCATG 
GAGACGGCGA GCTTTAAGAA GAAACGCTCC AAATCCGCAG ACATCTGGCG GGAGGACAGC 
CTGGAATTCT CACTGTCTGA TCTGAGCCAA GAACATTTAA CAAGCAACGA AGAGATCTTG 
GGTTCCGCCG AGGAGAAAGA CTGCGAGGAG GCTCGGGCGA TGGAAACCCG GGCGAGTCCG 
CGGCAGCTCA GTGCCTGTCA ACGGGCCAAT TCTCTGGGGG ACTTGTACGC TCAGAAGAAC 
CCTGGGGTGA CGGCAAACGG GGGGCCCAGG AGCAAACTTG CAGGCTACTG TCGGAATTTG 
GTGTCTGACA TCCCTGATCT CGCAAACCAT AAGATGCCAC CAGCTGCCGC TGAAGAGGCT 
CTCCCCTACA GTAATTATAA CACACTCCCC TGTAGGAAAT CTCACTGTCT CTCTGAAGGT 
GCCACCAACC CACAGATTAG CCATAGCAGC AGCATGCAAG GCAGAAGAGC AAAAACCACT 
CAGGATGTTA ACACAGGCGA GGGTAGCGAG TTTGCTGACA GCGGGATCGA AGGGGCCACC 
ACCGACACGG ACCTCCTGTC CCGGCGATCC AATGCCACTA ACTCCAGCTA CTCGCCCCCC 
ACCGGCCGTG CCTTTGTGGG CAGCGACAGC GGCAGCAGCT CCACCGGGGA CGCGGCCCGC 
CAGGGGGTGT ATGAGAATTT TCGCCGGGAG CTGGAGATGA GCACCACGAA CAGCGAGAGC 
CTGGAGGAGG CGGGCTCCGC GCACAGCGAC GAGCAGAGCA GCGGCACCCT GAGCTCCCCG 
GGCCAGTCCG ACATCCTGCT CACGGCCGCC CAGGGCACAG TGCGCAAGGC CGGCGCCCTG 
GCCGTCAAGA ACTTCCTGGT GCACAAGAAG AACAAGAAGG TGGAGTCAGC CACGCGGAGG 
AAGTGGAAGC ACTACTGGGT GTCCTTGAAA GGGTGCACAC TCTTCTTCTA TGAGGGCGAC 
GGCAGGTCCG GGATAGACCA CAACAGCGTC CCCAAGCACG CCGTCTGGGT GGAGAACAGC 
ATTGTACAGG CAGTTCCGGA GCACCCCAAG AAGGACTTCG TCTTCTGCCT CAGCAATTCC 
CTGGGCGATG CCTTCCTCTT CCAGACCACC AGCCAGACGG AGCTGGAGAA CTGGATCACC 
GCCATCCACT CTGCTTGCGC GGCCGCTGTC GCAAGACACC ACCACAAAGA GGACACGCTC 
CGGCTGCTGA AGTCGGAGAT CAAAAAACTG GAACAAAAGA TTGACATGGA CGAGAAGATG 
AAGAAAATGG GCGAAATGCA GCTGTCTTCC GTCACGGATT CAAAGAAGAA GAAAACTATA 
TTAGATCAGA TCTTTGTCTG GGAGCAGAAT CTTGAGCAGT TCCAAATGGA CCTGTTTCGG 
TACCGCTGTT ACCTAGCCAG CCTCCAAGGC GGAGAGCTGC CCAACCCTAA AAGGTTACTT 
GCCTTTGCAA GCAGACCAAC CAAAGTGGCA ATGGGCCGCC TTGGAATTTT TTCCGTGTCA 
TCTTTTCATG CCCTGGTGGC TGCCCGCACT GGTGAAACTG GAGTCAGAAG ACGTACTCAG 
GCCATGTCCC GATCTGCAAG CAAGAGAAGG AGCAGGTTTT CTTCTCTTTG GGGTCTGGAC 
ACTACCTCTA AAAAGAAGCA GGGACGCCCA AGCATCAATC AGGTGTTCGG TGAGGGAACT 
GAGGCTGTAA AGAAATCTTT AGCGGGAGTA TTTGATGACA CTGTTCCAGA TGGCAAGAGG 
GAGAAAGAAA TGGTCTTACC TGCCGTCCAC CAGCACAATC CTGACTGTGA CATTTGGGTC 
CACGAGTATT TCACCCCATC CTGGTTCTGT CTACCGAACA ATCAGCCAGC CCTGACGGTC 
GTCCGGCCGG GGGACACGGC CCGGGATACC CTGGAGCTGA TCTGCAAGGC GCATAAACTG 
GATCATTCTG CTCATTACCT GCGCCTGAAA TTTCTAATAG AAAACAAAAT GCAGCATTAT 
GTGCCAAAGC CCGAGGAGGA CATTTATGAG CTGCTGTACA AAGAAATTGA AATTTGTCCA 
AAAGTCACTC AGAACATCCA GATTGAGAAG TCAGATACAG CTGGTGATAA TTATGGGTTT 
TCACTTTCTT CTGTGGAAGA GGATGGTGTT CGAAGGCTCT ACGTGAATAG TGTAAAAGAA 
ACTGGCTTAG CTTCCAAGAA AGGCCTGAAG GCGGGAGATG AGATTCTTGA GATCAATAAG 
CGGGCTGCAG GCACCCTGAA CTCCTCTGTG CTCAGAGATT TCCTCACACA GCCGTCCCTG 
GGCCTCCTGG TGAGGACCTG CCCCGAGCTG GAGGGAGGAG CAGAGCTGCT GGAGAACCCG 
CCCCATCGGC TGGACGGCCC GGCTGACCTC GGCGAGAGCC CCCTCGCCTT TCTCAGCAGT 
GACACAGGCC ACAGCCTCTG CAGCGAGCAG GGCAGCAGTG CTGAGGCTGC TCCGGAGGAG 
GTGGAAGGGC CGGACTTGGA ATCCTCAGAT GATACTGATC ACAGCAGCAA GAGCACGGAG 
CAGGTGGCCG CATTCTGCCG TAGTCTGCAC GAGATGAAGC CCTCTGACCC GAGCCCGTCC 
CCCCAGGACT GCACTGGGCC TCAGCTGGCC GCCCTGAGGC AGCTCACGGA TGCCGACAAG 
CTGCGCAAGG TCATCTGCGA GCTCCTGGAG ACAGAGCGCA CCTACGTGAA GGACTTAAAC 
TGTCTCATGG AGAGATACCT AAAACCTCTT CAAAAAGAAA CTTTTCTCAC CCAGGATGAG 
CTTGATGTGC TTTTTGGAAA TTTAACCGAA ATGGTAGAGT TTCAAGTAGA ATTCCTTAAA 
ACTCTGGAAG ATGGAGTGAG ATTGGTACCT GATTTGGAAA AGCTTGAGAA AGTTGATCAA 
TTTAAGAAAG TGCTGTTCTC CCTGGGGGGA TCCTTTCTGT ATTACGCTGA CCGCTTCAAG 
CTCTACAGTG CCTTCTGCGC CAGCCACACG AAGGTCCCCA AGGTCCTGGT GAAAGCCAAG 
ACGGACGCAG CTTTCAAGGC GTTCCTGGAC GCCCAGAACC CAAAGCAGCA GCACTCGTCC 
ACCCTGGAGT CTTACCTCAT CAAGCCCATC CAGAGGATCC TTAAGTATCC GCTTCTGCTG 
AAGGAGCTTT TTGCTCTGAC GGACGCGGAG AGTGAGGAAC ACTACCACCT GGATGTGGCC 
ATCAAGACTA TGAACAAGGT TGCAAGTCAT ATCAATGAAA TGCAGAAAAT CCATGAGGAA 
TTTGGGGCTG TGTTTGACCA GCTGATTGCT GAACAGACCG GTGAGAAAAA AGAGGTTGCG 
GATCTGAGCA TGGGAGACCT GCTGTTGCAT ACCACCGTCA TCTGGCCAAA CCCGCCCGCC 
TCACTGGGCA AGTGGAAAAA GGAGCCTGAA TTGGCAGCAT TTGTCTTCAA AACTGCCGTG 
GTCCTTGTCT ATAAAGATGG TACCAAACAG AAGAAGAAAC TTGTAGGATC ACACAGGCTT 
TCAATTTACG AGGACTGGGA CCCCTTCCGA TTTCGACACA TGATTCCTAC TGAAGCTCTG 
CAGGTTCGCG CTCTGGCAAG TGCAGATGCA GAAGCAAATG CCGTGTGTGA AATCGTCCAT 
GTAAAGTCGG AGTCGGAAGG GAGGCCGGAG AGGGTCTTCC ACCTGTGCTG CAGCTCCCCA 
GAGAGCCGAA AGGATTTCCT GAAGGCTGTG CATTCCATCC TGCGAGATAA GCACAGAAGA 
CAGCTCCTGA AGACTGAGAG CCTTCCCTCG TCCCAGCAAT ATGTCCCTTT CGGAGGAAAG 
CGGTTGTGTG CGCTGAAAGG GGCCAGGCCG GCTATGAGCA GGGCAGTGTC CGCCCCAAGC 
AAGTCTCTTG GGAGGAGGAG GCGGCGACTG GCCCGAAACA GGTTTACCAT TGATTCCGAT 
GCCATCTCCG CCAGCAGCCC GGAGAAAGAC CCCCAGCAGC CCCCTGGTGG TGGGGACACT 
GACCGCTGGG TGGAGGAACA GTTTGATCTT GCTCAGTATG AAGAGCAGGA TGACATCAAG 
GAGACAGACA TCCTCAGTGA CGATGATGAG TTCTGTGAGT CGGCCAAGGG CGCCTCGGCG 
GACAGAGACC TGCAGGAGCG GCTTCAGGCT GCCTCCATCA CTCAGCGGGA GAAAGGCCGG 
AAGCCCCTGG ACAGCCACGC GTCCCGCATG ACGCAGCTCA AGAAGCAAGC CGCCCTGTCG 
GGCATCAACG GAGGCCCGGA GAGTACGAGC GAGGAGGTCA TTTGGGTTAG GCGCGAAGAC 
TTTGCCCCCT CCCGCAAGCT GAACACTGAG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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TIAM1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

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