GALC cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	GALC
Entrez Gene ID	102412612
Full Name	galactosylceramidase
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006061465.2	XP_006061527.2	galactocerebrosidase isoform X1
XM_006061467.2	XP_006061529.1	galactocerebrosidase isoform X3
XM_006061465.1	XP_006061527.1	galactocerebrosidase isoform X1
XM_006061467.1	XP_006061529.1	galactocerebrosidase isoform X3
XM_006061466.1	XP_006061528.1	galactocerebrosidase isoform X2
XM_025295139.1	XP_025150924.1	galactocerebrosidase isoform X2

Pathways from BioSystems
Gene Ontology

The following GALC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GALC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu17721	XM_006061465.1 Latest version!	Bubalus bubalis galactosylceramidase (GALC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu17722	XM_006061466.1 Latest version!	Bubalus bubalis galactosylceramidase (GALC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu17723	XM_006061467.2 Latest version!	Bubalus bubalis galactosylceramidase (GALC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBu44408	XM_006061465.2 Latest version!	Bubalus bubalis galactosylceramidase (GALC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBu44409	XM_025295139.1 Latest version!	Bubalus bubalis galactosylceramidase (GALC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OBu17723	XM_006061467.1	Bubalus bubalis galactosylceramidase (GALC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GALC ( XM_006061465.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006061527.1 Bubalus bubalis galactosylceramidase (GALC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu17721
Clone ID Related Accession (Same CDS sequence)	XM_006061465.1
Accession Version	XM_006061465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	galactocerebrosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785008.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCACAG AGGCGGGGCA CCGAAGTGAC AAACGCGGAA ACCCGCAGTC AAACCCTGAC 
GTAGAGCCCG CTAGAAAAGG CTCGCTCCCC ACCCCCCACA ACACGAAGGC GGCTGCGTCA 
GCGACCCGCA GCCCACACTC AACATGGCGG CGGCGGCACC CGCGGCCTCC TGCCCTTCTC 
CATCATGGCG GCGACCGGAC CCGCCTCCCT GGGCGCCCGA GTCATATGAC CTGTCACACA 
ATGGCTGAGC GCCTAATTCA GGCCTCCTGG CAACGCCGGG CGAAAGCCAT GACGGCCGCC 
GCGGGTTTGG CGGGCCAAGC CGCGGTGCCC CTGCTGCTGT GCGCGCTGCT GGCACCCAGC 
GGCGCGTACG TGCTCGACGA CTCCGAGGGG CTGGGCCGGG AGTTCGACGG CATCGGAGCT 
GTCAGCGGCG GCGGGGCAAC CTCCCGACTT CTAGTAAATT ACCCAGAGCC CTACCGTTCT 
GAAATACTGG ATTATCTCTT TAAGCCAAAC TTCGGTGCCT CATTGCATAT TCTAAAAGTT 
GAAATAGGTG GTGATGGGCA GACAACAGAT GGCACAGAGC CCTCCCACAT GCACTATGCA 
CTGGATGAGA ATTATTTCCG AGGGTATGAA TGGTGGTTGA TGAAGGAAGC TAAGAAGCGG 
AACCCTAATA TTACACTCAT AGGGCTGCCA TGGTCGTTCC CTGGATGGCT GGGGAAGGGC 
TTCAATTGGC CTTATGTCAA TCTCCAGCTG ACCGCCTACT ACATCGTGAC CTGGATTGTG 
GGCTCCAAGC ATTATCATGA CCTGGACATT GATTATATCG GAATTTGGAA TGAGAGGGCA 
TTTGACATTA ATTATATCAA GGTGTTAAGA AGAATGCTGA ACCATCAAGG TCTCCAGCAC 
GTGAAAATCA TAGCGAGTGA TAATCTCTGG GAGCCCATTT CTGCTTCTAT GCTGCTTGAC 
CCTGAGCTCT TGAAGGTGAT TGATGTAATA GGAGCTCATT ACCCTGGGAC ACTTACAGTA 
AAGGATGCTA GGTTGACTAA GAAGAAGCTT TGGTCTTCTG AAGATTTTAG CACTGTAAAT 
GGTGATATGG GTGCAGGCTG CTGGGGTCGC ATATTGAATC AGAATTACGT CAATGGCTAC 
ATGACTTCCA CCATTGCTTG GAACTTGGTG GCCAGTTACT ATCAGCAGTT GCCATATGGG 
CGTTGTGGAC TGATGACTGC TCAGGAGCCA TGGAGTGGGC ACTACGTGGT GGAAGCTCCT 
ATCTGGGTAT CAGCTCATAC TACTCAGTTT ACTCAACCAG GTTGGTATTA CCTGAAGACA 
GTTGGCCATT TAGAGAAAGG AGGAAGCTAC GTAGCTCTGA CGGATGGCTT AGGTAACCTC 
ACTGTCATCG TTGAAACTAT GAGTTATAAA CATTCCAAGT GTATACGACC ACTTCTTCCC 
TATTTCAATG TGTCACATCA GTTTGCAACG TTTGATCTTA AGGGTTCTTT TAGTCAAATA 
CCAGAGCTAC AGGTGTGGTA TACCAAACTC GGAAAACCAT CGGAGAGATT TCTCTTCAAA 
CAACTGGATT CTCTATGGCT GCTCGACAGC GGTGGCAGCT TCACGCTGGA GCTGCAGGAG 
GACGAGCTGT TCACCCTCAC CACGCTCACC TCCGGCCGCA AAGGCAGCTT CCCGCCGCCT 
CCCAAGTCCC AGCGCTTCCC CTCTGTCTAT GAGGATAACT TCAACATTGA TTACCCGTTT 
TTTAGTGAAG CTCCGAATTT TGCTGACCAG ACTGGGGTGT TTGAATACTA CGTGAATATG 
GGAGACCCCG GAGACCATCG CTTCACTCTG CGGCAGGTTC TCAATCAGAG GCCCATCACG 
TGGGCTGCCG ACGCGTTCAA CACCATCAGC GTTATCGGGG ATTACACGTG GTCCAATCTG 
ACTATAAAGT GTGATGTGTA CATAGAGACC CCTGAGAAGG GAGGCGTGTT CATTGCTGGA 
AGAGTCAATA AAGGTGGTAT TTTGATTAGA AGTGCCAGGG GGATTTTCTT CTGGATTTTT 
GCAAATGGAA CCTACAGAGT CACAGGCGAT CTAGCTGGAT GGATCATATA TAGTTTAGGA 
CATGCTGATG TTACAGCAAA AAAATGGTAT ACACTCACTT TAACTCTGAA TGGTCGTTTC 
TCCTCTGGCA AGCTGAATGG CAAGACCCTC TGGAAGAACA TCTCTGTGAA CTTCCCAGAA 
AACGGCTGGG CCGCGATTGG GACTCACTCC TTTGAATTTG CGCAATTTGA CAACTTTCGT 
GTGGAAGCCT CACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006061527.1
CDS	158..2455
Translation	MLTEAGHRSDKRGNPQSNPDVEPARKGSLPTPHNTKAAASATRSPHSTWRRRHPRPPALLHHGGDRTRLPGRPSHMTCHTMAERLIQASWQRRAKAMTAAAGLAGQAAVPLLLCALLAPSGAYVLDDSEGLGREFDGIGAVSGGGATSRLLVNYPEPYRSEILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFNWPYVNLQLTAYYIVTWIVGSKHYHDLDIDYIGIWNERAFDINYIKVLRRMLNHQGLQHVKIIASDNLWEPISASMLLDPELLKVIDVIGAHYPGTLTVKDARLTKKKLWSSEDFSTVNGDMGAGCWGRILNQNYVNGYMTSTIAWNLVASYYQQLPYGRCGLMTAQEPWSGHYVVEAPIWVSAHTTQFTQPGWYYLKTVGHLEKGGSYVALTDGLGNLTVIVETMSYKHSKCIRPLLPYFNVSHQFATFDLKGSFSQIPELQVWYTKLGKPSERFLFKQLDSLWLLDSGGSFTLELQEDELFTLTTLTSGRKGSFPPPPKSQRFPSVYEDNFNIDYPFFSEAPNFADQTGVFEYYVNMGDPGDHRFTLRQVLNQRPITWAADAFNTISVIGDYTWSNLTIKCDVYIETPEKGGVFIAGRVNKGGILIRSARGIFFWIFANGTYRVTGDLAGWIIYSLGHADVTAKKWYTLTLTLNGRFSSGKLNGKTLWKNISVNFPENGWAAIGTHSFEFAQFDNFRVEASH

Target ORF information:

RefSeq Version	XM_006061465.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis galactosylceramidase (GALC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006061465.1

ORF Insert Sequence:

ATGCTCACAG AGGCGGGGCA CCGAAGTGAC AAACGCGGAA ACCCGCAGTC AAACCCTGAC 
GTAGAGCCCG CTAGAAAAGG CTCGCTCCCC ACCCCCCACA ACACGAAGGC GGCTGCGTCA 
GCGACCCGCA GCCCACACTC AACATGGCGG CGGCGGCACC CGCGGCCTCC TGCCCTTCTC 
CATCATGGCG GCGACCGGAC CCGCCTCCCT GGGCGCCCGA GTCATATGAC CTGTCACACA 
ATGGCTGAGC GCCTAATTCA GGCCTCCTGG CAACGCCGGG CGAAAGCCAT GACGGCCGCC 
GCGGGTTTGG CGGGCCAAGC CGCGGTGCCC CTGCTGCTGT GCGCGCTGCT GGCACCCAGC 
GGCGCGTACG TGCTCGACGA CTCCGAGGGG CTGGGCCGGG AGTTCGACGG CATCGGAGCT 
GTCAGCGGCG GCGGGGCAAC CTCCCGACTT CTAGTAAATT ACCCAGAGCC CTACCGTTCT 
GAAATACTGG ATTATCTCTT TAAGCCAAAC TTCGGTGCCT CATTGCATAT TCTAAAAGTT 
GAAATAGGTG GTGATGGGCA GACAACAGAT GGCACAGAGC CCTCCCACAT GCACTATGCA 
CTGGATGAGA ATTATTTCCG AGGGTATGAA TGGTGGTTGA TGAAGGAAGC TAAGAAGCGG 
AACCCTAATA TTACACTCAT AGGGCTGCCA TGGTCGTTCC CTGGATGGCT GGGGAAGGGC 
TTCAATTGGC CTTATGTCAA TCTCCAGCTG ACCGCCTACT ACATCGTGAC CTGGATTGTG 
GGCTCCAAGC ATTATCATGA CCTGGACATT GATTATATCG GAATTTGGAA TGAGAGGGCA 
TTTGACATTA ATTATATCAA GGTGTTAAGA AGAATGCTGA ACCATCAAGG TCTCCAGCAC 
GTGAAAATCA TAGCGAGTGA TAATCTCTGG GAGCCCATTT CTGCTTCTAT GCTGCTTGAC 
CCTGAGCTCT TGAAGGTGAT TGATGTAATA GGAGCTCATT ACCCTGGGAC ACTTACAGTA 
AAGGATGCTA GGTTGACTAA GAAGAAGCTT TGGTCTTCTG AAGATTTTAG CACTGTAAAT 
GGTGATATGG GTGCAGGCTG CTGGGGTCGC ATATTGAATC AGAATTACGT CAATGGCTAC 
ATGACTTCCA CCATTGCTTG GAACTTGGTG GCCAGTTACT ATCAGCAGTT GCCATATGGG 
CGTTGTGGAC TGATGACTGC TCAGGAGCCA TGGAGTGGGC ACTACGTGGT GGAAGCTCCT 
ATCTGGGTAT CAGCTCATAC TACTCAGTTT ACTCAACCAG GTTGGTATTA CCTGAAGACA 
GTTGGCCATT TAGAGAAAGG AGGAAGCTAC GTAGCTCTGA CGGATGGCTT AGGTAACCTC 
ACTGTCATCG TTGAAACTAT GAGTTATAAA CATTCCAAGT GTATACGACC ACTTCTTCCC 
TATTTCAATG TGTCACATCA GTTTGCAACG TTTGATCTTA AGGGTTCTTT TAGTCAAATA 
CCAGAGCTAC AGGTGTGGTA TACCAAACTC GGAAAACCAT CGGAGAGATT TCTCTTCAAA 
CAACTGGATT CTCTATGGCT GCTCGACAGC GGTGGCAGCT TCACGCTGGA GCTGCAGGAG 
GACGAGCTGT TCACCCTCAC CACGCTCACC TCCGGCCGCA AAGGCAGCTT CCCGCCGCCT 
CCCAAGTCCC AGCGCTTCCC CTCTGTCTAT GAGGATAACT TCAACATTGA TTACCCGTTT 
TTTAGTGAAG CTCCGAATTT TGCTGACCAG ACTGGGGTGT TTGAATACTA CGTGAATATG 
GGAGACCCCG GAGACCATCG CTTCACTCTG CGGCAGGTTC TCAATCAGAG GCCCATCACG 
TGGGCTGCCG ACGCGTTCAA CACCATCAGC GTTATCGGGG ATTACACGTG GTCCAATCTG 
ACTATAAAGT GTGATGTGTA CATAGAGACC CCTGAGAAGG GAGGCGTGTT CATTGCTGGA 
AGAGTCAATA AAGGTGGTAT TTTGATTAGA AGTGCCAGGG GGATTTTCTT CTGGATTTTT 
GCAAATGGAA CCTACAGAGT CACAGGCGAT CTAGCTGGAT GGATCATATA TAGTTTAGGA 
CATGCTGATG TTACAGCAAA AAAATGGTAT ACACTCACTT TAACTCTGAA TGGTCGTTTC 
TCCTCTGGCA AGCTGAATGG CAAGACCCTC TGGAAGAACA TCTCTGTGAA CTTCCCAGAA 
AACGGCTGGG CCGCGATTGG GACTCACTCC TTTGAATTTG CGCAATTTGA CAACTTTCGT 
GTGGAAGCCT CACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GALC ( XM_006061466.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006061528.1 Bubalus bubalis galactosylceramidase (GALC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu17722
Clone ID Related Accession (Same CDS sequence)	XM_006061466.1
Accession Version	XM_006061466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	galactocerebrosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785008.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCACAG AGGCGGGGCA CCGAAGTGAC AAACGCGGAA ACCCGCAGTC AAACCCTGAC 
GTAGAGCCCG CTAGAAAAGG CTCGCTCCCC ACCCCCCACA ACACGAAGGC GGCTGCGTCA 
GCGACCCGCA GCCCACACTC AACATGGCGG CGGCGGCACC CGCGGCCTCC TGCCCTTCTC 
CATCATGGCG GCGACCGGAC CCGCCTCCCT GGGCGCCCGA GTCATATGAC CTGTCACACA 
ATGGCTGAGC GCCTAATTCA GGCCTCCTGG CAACGCCGGG CGAAAGCCAT GACGGCCGCC 
GCGGGTTTGG CGGGCCAAGC CGCGGTGCCC CTGCTGCTGT GCGCGCTGCT GGCACCCAGC 
GGCGCGTACG TGCTCGACGA CTCCGAGGGG CTGGGCCGGG AGTTCGACGG CATCGGAGCT 
GTCAGCGGCG GCGGGCCAAA CTTCGGTGCC TCATTGCATA TTCTAAAAGT TGAAATAGGT 
GGTGATGGGC AGACAACAGA TGGCACAGAG CCCTCCCACA TGCACTATGC ACTGGATGAG 
AATTATTTCC GAGGGTATGA ATGGTGGTTG ATGAAGGAAG CTAAGAAGCG GAACCCTAAT 
ATTACACTCA TAGGGCTGCC ATGGTCGTTC CCTGGATGGC TGGGGAAGGG CTTCAATTGG 
CCTTATGTCA ATCTCCAGCT GACCGCCTAC TACATCGTGA CCTGGATTGT GGGCTCCAAG 
CATTATCATG ACCTGGACAT TGATTATATC GGAATTTGGA ATGAGAGGGC ATTTGACATT 
AATTATATCA AGGTGTTAAG AAGAATGCTG AACCATCAAG GTCTCCAGCA CGTGAAAATC 
ATAGCGAGTG ATAATCTCTG GGAGCCCATT TCTGCTTCTA TGCTGCTTGA CCCTGAGCTC 
TTGAAGGTGA TTGATGTAAT AGGAGCTCAT TACCCTGGGA CACTTACAGT AAAGGATGCT 
AGGTTGACTA AGAAGAAGCT TTGGTCTTCT GAAGATTTTA GCACTGTAAA TGGTGATATG 
GGTGCAGGCT GCTGGGGTCG CATATTGAAT CAGAATTACG TCAATGGCTA CATGACTTCC 
ACCATTGCTT GGAACTTGGT GGCCAGTTAC TATCAGCAGT TGCCATATGG GCGTTGTGGA 
CTGATGACTG CTCAGGAGCC ATGGAGTGGG CACTACGTGG TGGAAGCTCC TATCTGGGTA 
TCAGCTCATA CTACTCAGTT TACTCAACCA GGTTGGTATT ACCTGAAGAC AGTTGGCCAT 
TTAGAGAAAG GAGGAAGCTA CGTAGCTCTG ACGGATGGCT TAGGTAACCT CACTGTCATC 
GTTGAAACTA TGAGTTATAA ACATTCCAAG TGTATACGAC CACTTCTTCC CTATTTCAAT 
GTGTCACATC AGTTTGCAAC GTTTGATCTT AAGGGTTCTT TTAGTCAAAT ACCAGAGCTA 
CAGGTGTGGT ATACCAAACT CGGAAAACCA TCGGAGAGAT TTCTCTTCAA ACAACTGGAT 
TCTCTATGGC TGCTCGACAG CGGTGGCAGC TTCACGCTGG AGCTGCAGGA GGACGAGCTG 
TTCACCCTCA CCACGCTCAC CTCCGGCCGC AAAGGCAGCT TCCCGCCGCC TCCCAAGTCC 
CAGCGCTTCC CCTCTGTCTA TGAGGATAAC TTCAACATTG ATTACCCGTT TTTTAGTGAA 
GCTCCGAATT TTGCTGACCA GACTGGGGTG TTTGAATACT ACGTGAATAT GGGAGACCCC 
GGAGACCATC GCTTCACTCT GCGGCAGGTT CTCAATCAGA GGCCCATCAC GTGGGCTGCC 
GACGCGTTCA ACACCATCAG CGTTATCGGG GATTACACGT GGTCCAATCT GACTATAAAG 
TGTGATGTGT ACATAGAGAC CCCTGAGAAG GGAGGCGTGT TCATTGCTGG AAGAGTCAAT 
AAAGGTGGTA TTTTGATTAG AAGTGCCAGG GGGATTTTCT TCTGGATTTT TGCAAATGGA 
ACCTACAGAG TCACAGGCGA TCTAGCTGGA TGGATCATAT ATAGTTTAGG ACATGCTGAT 
GTTACAGCAA AAAAATGGTA TACACTCACT TTAACTCTGA ATGGTCGTTT CTCCTCTGGC 
AAGCTGAATG GCAAGACCCT CTGGAAGAAC ATCTCTGTGA ACTTCCCAGA AAACGGCTGG 
GCCGCGATTG GGACTCACTC CTTTGAATTT GCGCAATTTG ACAACTTTCG TGTGGAAGCC 
TCACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006061528.1
CDS	158..2386
Translation	MLTEAGHRSDKRGNPQSNPDVEPARKGSLPTPHNTKAAASATRSPHSTWRRRHPRPPALLHHGGDRTRLPGRPSHMTCHTMAERLIQASWQRRAKAMTAAAGLAGQAAVPLLLCALLAPSGAYVLDDSEGLGREFDGIGAVSGGGPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFNWPYVNLQLTAYYIVTWIVGSKHYHDLDIDYIGIWNERAFDINYIKVLRRMLNHQGLQHVKIIASDNLWEPISASMLLDPELLKVIDVIGAHYPGTLTVKDARLTKKKLWSSEDFSTVNGDMGAGCWGRILNQNYVNGYMTSTIAWNLVASYYQQLPYGRCGLMTAQEPWSGHYVVEAPIWVSAHTTQFTQPGWYYLKTVGHLEKGGSYVALTDGLGNLTVIVETMSYKHSKCIRPLLPYFNVSHQFATFDLKGSFSQIPELQVWYTKLGKPSERFLFKQLDSLWLLDSGGSFTLELQEDELFTLTTLTSGRKGSFPPPPKSQRFPSVYEDNFNIDYPFFSEAPNFADQTGVFEYYVNMGDPGDHRFTLRQVLNQRPITWAADAFNTISVIGDYTWSNLTIKCDVYIETPEKGGVFIAGRVNKGGILIRSARGIFFWIFANGTYRVTGDLAGWIIYSLGHADVTAKKWYTLTLTLNGRFSSGKLNGKTLWKNISVNFPENGWAAIGTHSFEFAQFDNFRVEASH

Target ORF information:

RefSeq Version	XM_006061466.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis galactosylceramidase (GALC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006061466.1

ORF Insert Sequence:

ATGCTCACAG AGGCGGGGCA CCGAAGTGAC AAACGCGGAA ACCCGCAGTC AAACCCTGAC 
GTAGAGCCCG CTAGAAAAGG CTCGCTCCCC ACCCCCCACA ACACGAAGGC GGCTGCGTCA 
GCGACCCGCA GCCCACACTC AACATGGCGG CGGCGGCACC CGCGGCCTCC TGCCCTTCTC 
CATCATGGCG GCGACCGGAC CCGCCTCCCT GGGCGCCCGA GTCATATGAC CTGTCACACA 
ATGGCTGAGC GCCTAATTCA GGCCTCCTGG CAACGCCGGG CGAAAGCCAT GACGGCCGCC 
GCGGGTTTGG CGGGCCAAGC CGCGGTGCCC CTGCTGCTGT GCGCGCTGCT GGCACCCAGC 
GGCGCGTACG TGCTCGACGA CTCCGAGGGG CTGGGCCGGG AGTTCGACGG CATCGGAGCT 
GTCAGCGGCG GCGGGCCAAA CTTCGGTGCC TCATTGCATA TTCTAAAAGT TGAAATAGGT 
GGTGATGGGC AGACAACAGA TGGCACAGAG CCCTCCCACA TGCACTATGC ACTGGATGAG 
AATTATTTCC GAGGGTATGA ATGGTGGTTG ATGAAGGAAG CTAAGAAGCG GAACCCTAAT 
ATTACACTCA TAGGGCTGCC ATGGTCGTTC CCTGGATGGC TGGGGAAGGG CTTCAATTGG 
CCTTATGTCA ATCTCCAGCT GACCGCCTAC TACATCGTGA CCTGGATTGT GGGCTCCAAG 
CATTATCATG ACCTGGACAT TGATTATATC GGAATTTGGA ATGAGAGGGC ATTTGACATT 
AATTATATCA AGGTGTTAAG AAGAATGCTG AACCATCAAG GTCTCCAGCA CGTGAAAATC 
ATAGCGAGTG ATAATCTCTG GGAGCCCATT TCTGCTTCTA TGCTGCTTGA CCCTGAGCTC 
TTGAAGGTGA TTGATGTAAT AGGAGCTCAT TACCCTGGGA CACTTACAGT AAAGGATGCT 
AGGTTGACTA AGAAGAAGCT TTGGTCTTCT GAAGATTTTA GCACTGTAAA TGGTGATATG 
GGTGCAGGCT GCTGGGGTCG CATATTGAAT CAGAATTACG TCAATGGCTA CATGACTTCC 
ACCATTGCTT GGAACTTGGT GGCCAGTTAC TATCAGCAGT TGCCATATGG GCGTTGTGGA 
CTGATGACTG CTCAGGAGCC ATGGAGTGGG CACTACGTGG TGGAAGCTCC TATCTGGGTA 
TCAGCTCATA CTACTCAGTT TACTCAACCA GGTTGGTATT ACCTGAAGAC AGTTGGCCAT 
TTAGAGAAAG GAGGAAGCTA CGTAGCTCTG ACGGATGGCT TAGGTAACCT CACTGTCATC 
GTTGAAACTA TGAGTTATAA ACATTCCAAG TGTATACGAC CACTTCTTCC CTATTTCAAT 
GTGTCACATC AGTTTGCAAC GTTTGATCTT AAGGGTTCTT TTAGTCAAAT ACCAGAGCTA 
CAGGTGTGGT ATACCAAACT CGGAAAACCA TCGGAGAGAT TTCTCTTCAA ACAACTGGAT 
TCTCTATGGC TGCTCGACAG CGGTGGCAGC TTCACGCTGG AGCTGCAGGA GGACGAGCTG 
TTCACCCTCA CCACGCTCAC CTCCGGCCGC AAAGGCAGCT TCCCGCCGCC TCCCAAGTCC 
CAGCGCTTCC CCTCTGTCTA TGAGGATAAC TTCAACATTG ATTACCCGTT TTTTAGTGAA 
GCTCCGAATT TTGCTGACCA GACTGGGGTG TTTGAATACT ACGTGAATAT GGGAGACCCC 
GGAGACCATC GCTTCACTCT GCGGCAGGTT CTCAATCAGA GGCCCATCAC GTGGGCTGCC 
GACGCGTTCA ACACCATCAG CGTTATCGGG GATTACACGT GGTCCAATCT GACTATAAAG 
TGTGATGTGT ACATAGAGAC CCCTGAGAAG GGAGGCGTGT TCATTGCTGG AAGAGTCAAT 
AAAGGTGGTA TTTTGATTAG AAGTGCCAGG GGGATTTTCT TCTGGATTTT TGCAAATGGA 
ACCTACAGAG TCACAGGCGA TCTAGCTGGA TGGATCATAT ATAGTTTAGG ACATGCTGAT 
GTTACAGCAA AAAAATGGTA TACACTCACT TTAACTCTGA ATGGTCGTTT CTCCTCTGGC 
AAGCTGAATG GCAAGACCCT CTGGAAGAAC ATCTCTGTGA ACTTCCCAGA AAACGGCTGG 
GCCGCGATTG GGACTCACTC CTTTGAATTT GCGCAATTTG ACAACTTTCG TGTGGAAGCC 
TCACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GALC ( XM_006061467.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006061529.1 Bubalus bubalis galactosylceramidase (GALC), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu17723
Clone ID Related Accession (Same CDS sequence)	XM_006061467.2 , XM_006061467.1
Accession Version	XM_006061467.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	galactocerebrosidase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006061467.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTTG CTCAGAACCT GTGTTTTAGC TGTTGGACAA ACATCAGCCC TATTTACCCA 
GCTATGAGCA AAACAAAGGC AACCTCCCGA CTTCTAGTAA ATTACCCAGA GCCCTACCGT 
TCTGAAATAC TGGATTATCT CTTTAAGCCA AACTTCGGTG CCTCATTGCA TATTCTAAAA 
GTTGAAATAG GTGGTGATGG GCAGACAACA GATGGCACAG AGCCCTCCCA CATGCACTAT 
GCACTGGATG AGAATTATTT CCGAGGGTAT GAATGGTGGT TGATGAAGGA AGCTAAGAAG 
CGGAACCCTA ATATTACACT CATAGGGCTG CCATGGTCGT TCCCTGGATG GCTGGGGAAG 
GGCTTCAATT GGCCTTATGT CAATCTCCAG CTGACCGCCT ACTACATCGT GACCTGGATT 
GTGGGCTCCA AGCATTATCA TGACCTGGAC ATTGATTATA TCGGAATTTG GAATGAGAGG 
GCATTTGACA TTAATTATAT CAAGGTGTTA AGAAGAATGC TGAACCATCA AGGTCTCCAG 
CACGTGAAAA TCATAGCGAG TGATAATCTC TGGGAGCCCA TTTCTGCTTC TATGCTGCTT 
GACCCTGAGC TCTTGAAGGT GATTGATGTA ATAGGAGCTC ATTACCCTGG GACACTTACA 
GTAAAGGATG CTAGGTTGAC TAAGAAGAAG CTTTGGTCTT CTGAAGATTT TAGCACTGTA 
AATGGTGATA TGGGTGCAGG CTGCTGGGGT CGCATATTGA ATCAGAATTA CGTCAATGGC 
TACATGACTT CCACCATTGC TTGGAACTTG GTGGCCAGTT ACTATCAGCA GTTGCCATAT 
GGGCGTTGTG GACTGATGAC TGCTCAGGAG CCATGGAGTG GGCACTACGT GGTGGAAGCT 
CCTATCTGGG TATCAGCTCA TACTACTCAG TTTACTCAAC CAGGTTGGTA TTACCTGAAG 
ACAGTTGGCC ATTTAGAGAA AGGAGGAAGC TACGTAGCTC TGACGGATGG CTTAGGTAAC 
CTCACTGTCA TCGTTGAAAC TATGAGTTAT AAACATTCCA AGTGTATACG ACCACTTCTT 
CCCTATTTCA ATGTGTCACA TCAGTTTGCA ACGTTTGATC TTAAGGGTTC TTTTAGTCAA 
ATACCAGAGC TACAGGTGTG GTATACCAAA CTCGGAAAAC CATCGGAGAG ATTTCTCTTC 
AAACAACTGG ATTCTCTATG GCTGCTCGAC AGCGGTGGCA GCTTCACGCT GGAGCTGCAG 
GAGGACGAGC TGTTCACCCT CACCACGCTC ACCTCCGGCC GCAAAGGCAG CTTCCCGCCG 
CCTCCCAAGT CCCAGCGCTT CCCCTCTGTC TATGAGGATA ACTTCAACAT TGATTACCCG 
TTTTTTAGTG AAGCTCCGAA TTTTGCTGAC CAGACTGGGG TGTTTGAATA CTACGTGAAT 
ATGGGAGACC CCGGAGACCA TCGCTTCACT CTGCGGCAGG TTCTCAATCA GAGGCCCATC 
ACGTGGGCTG CCGACGCGTT CAACACCATC AGCGTTATCG GGGATTACAC GTGGTCCAAT 
CTGACTATAA AGTGTGATGT GTACATAGAG ACCCCTGAGA AGGGAGGCGT GTTCATTGCT 
GGAAGAGTCA ATAAAGGTGG TATTTTGATT AGAAGTGCCA GGGGGATTTT CTTCTGGATT 
TTTGCAAATG GAACCTACAG AGTCACAGGC GATCTAGCTG GATGGATCAT ATATAGTTTA 
GGACATGCTG ATGTTACAGC AAAAAAATGG TATACACTCA CTTTAACTCT GAATGGTCGT 
TTCTCCTCTG GCAAGCTGAA TGGCAAGACC CTCTGGAAGA ACATCTCTGT GAACTTCCCA 
GAAAACGGCT GGGCCGCGAT TGGGACTCAC TCCTTTGAAT TTGCGCAATT TGACAACTTT 
CGTGTGGAAG CCTCACACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006061529.1
CDS	319..2259
Translation	MVVAQNLCFSCWTNISPIYPAMSKTKATSRLLVNYPEPYRSEILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFNWPYVNLQLTAYYIVTWIVGSKHYHDLDIDYIGIWNERAFDINYIKVLRRMLNHQGLQHVKIIASDNLWEPISASMLLDPELLKVIDVIGAHYPGTLTVKDARLTKKKLWSSEDFSTVNGDMGAGCWGRILNQNYVNGYMTSTIAWNLVASYYQQLPYGRCGLMTAQEPWSGHYVVEAPIWVSAHTTQFTQPGWYYLKTVGHLEKGGSYVALTDGLGNLTVIVETMSYKHSKCIRPLLPYFNVSHQFATFDLKGSFSQIPELQVWYTKLGKPSERFLFKQLDSLWLLDSGGSFTLELQEDELFTLTTLTSGRKGSFPPPPKSQRFPSVYEDNFNIDYPFFSEAPNFADQTGVFEYYVNMGDPGDHRFTLRQVLNQRPITWAADAFNTISVIGDYTWSNLTIKCDVYIETPEKGGVFIAGRVNKGGILIRSARGIFFWIFANGTYRVTGDLAGWIIYSLGHADVTAKKWYTLTLTLNGRFSSGKLNGKTLWKNISVNFPENGWAAIGTHSFEFAQFDNFRVEASH

Target ORF information:

RefSeq Version	XM_006061467.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis galactosylceramidase (GALC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006061467.2

ORF Insert Sequence:

ATGGTTGTTG CTCAGAACCT GTGTTTTAGC TGTTGGACAA ACATCAGCCC TATTTACCCA 
GCTATGAGCA AAACAAAGGC AACCTCCCGA CTTCTAGTAA ATTACCCAGA GCCCTACCGT 
TCTGAAATAC TGGATTATCT CTTTAAGCCA AACTTCGGTG CCTCATTGCA TATTCTAAAA 
GTTGAAATAG GTGGTGATGG GCAGACAACA GATGGCACAG AGCCCTCCCA CATGCACTAT 
GCACTGGATG AGAATTATTT CCGAGGGTAT GAATGGTGGT TGATGAAGGA AGCTAAGAAG 
CGGAACCCTA ATATTACACT CATAGGGCTG CCATGGTCGT TCCCTGGATG GCTGGGGAAG 
GGCTTCAATT GGCCTTATGT CAATCTCCAG CTGACCGCCT ACTACATCGT GACCTGGATT 
GTGGGCTCCA AGCATTATCA TGACCTGGAC ATTGATTATA TCGGAATTTG GAATGAGAGG 
GCATTTGACA TTAATTATAT CAAGGTGTTA AGAAGAATGC TGAACCATCA AGGTCTCCAG 
CACGTGAAAA TCATAGCGAG TGATAATCTC TGGGAGCCCA TTTCTGCTTC TATGCTGCTT 
GACCCTGAGC TCTTGAAGGT GATTGATGTA ATAGGAGCTC ATTACCCTGG GACACTTACA 
GTAAAGGATG CTAGGTTGAC TAAGAAGAAG CTTTGGTCTT CTGAAGATTT TAGCACTGTA 
AATGGTGATA TGGGTGCAGG CTGCTGGGGT CGCATATTGA ATCAGAATTA CGTCAATGGC 
TACATGACTT CCACCATTGC TTGGAACTTG GTGGCCAGTT ACTATCAGCA GTTGCCATAT 
GGGCGTTGTG GACTGATGAC TGCTCAGGAG CCATGGAGTG GGCACTACGT GGTGGAAGCT 
CCTATCTGGG TATCAGCTCA TACTACTCAG TTTACTCAAC CAGGTTGGTA TTACCTGAAG 
ACAGTTGGCC ATTTAGAGAA AGGAGGAAGC TACGTAGCTC TGACGGATGG CTTAGGTAAC 
CTCACTGTCA TCGTTGAAAC TATGAGTTAT AAACATTCCA AGTGTATACG ACCACTTCTT 
CCCTATTTCA ATGTGTCACA TCAGTTTGCA ACGTTTGATC TTAAGGGTTC TTTTAGTCAA 
ATACCAGAGC TACAGGTGTG GTATACCAAA CTCGGAAAAC CATCGGAGAG ATTTCTCTTC 
AAACAACTGG ATTCTCTATG GCTGCTCGAC AGCGGTGGCA GCTTCACGCT GGAGCTGCAG 
GAGGACGAGC TGTTCACCCT CACCACGCTC ACCTCCGGCC GCAAAGGCAG CTTCCCGCCG 
CCTCCCAAGT CCCAGCGCTT CCCCTCTGTC TATGAGGATA ACTTCAACAT TGATTACCCG 
TTTTTTAGTG AAGCTCCGAA TTTTGCTGAC CAGACTGGGG TGTTTGAATA CTACGTGAAT 
ATGGGAGACC CCGGAGACCA TCGCTTCACT CTGCGGCAGG TTCTCAATCA GAGGCCCATC 
ACGTGGGCTG CCGACGCGTT CAACACCATC AGCGTTATCG GGGATTACAC GTGGTCCAAT 
CTGACTATAA AGTGTGATGT GTACATAGAG ACCCCTGAGA AGGGAGGCGT GTTCATTGCT 
GGAAGAGTCA ATAAAGGTGG TATTTTGATT AGAAGTGCCA GGGGGATTTT CTTCTGGATT 
TTTGCAAATG GAACCTACAG AGTCACAGGC GATCTAGCTG GATGGATCAT ATATAGTTTA 
GGACATGCTG ATGTTACAGC AAAAAAATGG TATACACTCA CTTTAACTCT GAATGGTCGT 
TTCTCCTCTG GCAAGCTGAA TGGCAAGACC CTCTGGAAGA ACATCTCTGT GAACTTCCCA 
GAAAACGGCT GGGCCGCGAT TGGGACTCAC TCCTTTGAAT TTGCGCAATT TGACAACTTT 
CGTGTGGAAG CCTCACACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GALC ( XM_006061467.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006061529.1 Bubalus bubalis galactosylceramidase (GALC), transcript variant X3, mRNA.

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CloneID	OBu17723
Clone ID Related Accession (Same CDS sequence)	XM_006061467.2 , XM_006061467.1
Accession Version	XM_006061467.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	galactocerebrosidase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785008.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTTG CTCAGAACCT GTGTTTTAGC TGTTGGACAA ACATCAGCCC TATTTACCCA 
GCTATGAGCA AAACAAAGGC AACCTCCCGA CTTCTAGTAA ATTACCCAGA GCCCTACCGT 
TCTGAAATAC TGGATTATCT CTTTAAGCCA AACTTCGGTG CCTCATTGCA TATTCTAAAA 
GTTGAAATAG GTGGTGATGG GCAGACAACA GATGGCACAG AGCCCTCCCA CATGCACTAT 
GCACTGGATG AGAATTATTT CCGAGGGTAT GAATGGTGGT TGATGAAGGA AGCTAAGAAG 
CGGAACCCTA ATATTACACT CATAGGGCTG CCATGGTCGT TCCCTGGATG GCTGGGGAAG 
GGCTTCAATT GGCCTTATGT CAATCTCCAG CTGACCGCCT ACTACATCGT GACCTGGATT 
GTGGGCTCCA AGCATTATCA TGACCTGGAC ATTGATTATA TCGGAATTTG GAATGAGAGG 
GCATTTGACA TTAATTATAT CAAGGTGTTA AGAAGAATGC TGAACCATCA AGGTCTCCAG 
CACGTGAAAA TCATAGCGAG TGATAATCTC TGGGAGCCCA TTTCTGCTTC TATGCTGCTT 
GACCCTGAGC TCTTGAAGGT GATTGATGTA ATAGGAGCTC ATTACCCTGG GACACTTACA 
GTAAAGGATG CTAGGTTGAC TAAGAAGAAG CTTTGGTCTT CTGAAGATTT TAGCACTGTA 
AATGGTGATA TGGGTGCAGG CTGCTGGGGT CGCATATTGA ATCAGAATTA CGTCAATGGC 
TACATGACTT CCACCATTGC TTGGAACTTG GTGGCCAGTT ACTATCAGCA GTTGCCATAT 
GGGCGTTGTG GACTGATGAC TGCTCAGGAG CCATGGAGTG GGCACTACGT GGTGGAAGCT 
CCTATCTGGG TATCAGCTCA TACTACTCAG TTTACTCAAC CAGGTTGGTA TTACCTGAAG 
ACAGTTGGCC ATTTAGAGAA AGGAGGAAGC TACGTAGCTC TGACGGATGG CTTAGGTAAC 
CTCACTGTCA TCGTTGAAAC TATGAGTTAT AAACATTCCA AGTGTATACG ACCACTTCTT 
CCCTATTTCA ATGTGTCACA TCAGTTTGCA ACGTTTGATC TTAAGGGTTC TTTTAGTCAA 
ATACCAGAGC TACAGGTGTG GTATACCAAA CTCGGAAAAC CATCGGAGAG ATTTCTCTTC 
AAACAACTGG ATTCTCTATG GCTGCTCGAC AGCGGTGGCA GCTTCACGCT GGAGCTGCAG 
GAGGACGAGC TGTTCACCCT CACCACGCTC ACCTCCGGCC GCAAAGGCAG CTTCCCGCCG 
CCTCCCAAGT CCCAGCGCTT CCCCTCTGTC TATGAGGATA ACTTCAACAT TGATTACCCG 
TTTTTTAGTG AAGCTCCGAA TTTTGCTGAC CAGACTGGGG TGTTTGAATA CTACGTGAAT 
ATGGGAGACC CCGGAGACCA TCGCTTCACT CTGCGGCAGG TTCTCAATCA GAGGCCCATC 
ACGTGGGCTG CCGACGCGTT CAACACCATC AGCGTTATCG GGGATTACAC GTGGTCCAAT 
CTGACTATAA AGTGTGATGT GTACATAGAG ACCCCTGAGA AGGGAGGCGT GTTCATTGCT 
GGAAGAGTCA ATAAAGGTGG TATTTTGATT AGAAGTGCCA GGGGGATTTT CTTCTGGATT 
TTTGCAAATG GAACCTACAG AGTCACAGGC GATCTAGCTG GATGGATCAT ATATAGTTTA 
GGACATGCTG ATGTTACAGC AAAAAAATGG TATACACTCA CTTTAACTCT GAATGGTCGT 
TTCTCCTCTG GCAAGCTGAA TGGCAAGACC CTCTGGAAGA ACATCTCTGT GAACTTCCCA 
GAAAACGGCT GGGCCGCGAT TGGGACTCAC TCCTTTGAAT TTGCGCAATT TGACAACTTT 
CGTGTGGAAG CCTCACACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006061529.1
CDS	292..2232
Translation	MVVAQNLCFSCWTNISPIYPAMSKTKATSRLLVNYPEPYRSEILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFNWPYVNLQLTAYYIVTWIVGSKHYHDLDIDYIGIWNERAFDINYIKVLRRMLNHQGLQHVKIIASDNLWEPISASMLLDPELLKVIDVIGAHYPGTLTVKDARLTKKKLWSSEDFSTVNGDMGAGCWGRILNQNYVNGYMTSTIAWNLVASYYQQLPYGRCGLMTAQEPWSGHYVVEAPIWVSAHTTQFTQPGWYYLKTVGHLEKGGSYVALTDGLGNLTVIVETMSYKHSKCIRPLLPYFNVSHQFATFDLKGSFSQIPELQVWYTKLGKPSERFLFKQLDSLWLLDSGGSFTLELQEDELFTLTTLTSGRKGSFPPPPKSQRFPSVYEDNFNIDYPFFSEAPNFADQTGVFEYYVNMGDPGDHRFTLRQVLNQRPITWAADAFNTISVIGDYTWSNLTIKCDVYIETPEKGGVFIAGRVNKGGILIRSARGIFFWIFANGTYRVTGDLAGWIIYSLGHADVTAKKWYTLTLTLNGRFSSGKLNGKTLWKNISVNFPENGWAAIGTHSFEFAQFDNFRVEASH

Target ORF information:

RefSeq Version	XM_006061467.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis galactosylceramidase (GALC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006061467.1

ORF Insert Sequence:

ATGGTTGTTG CTCAGAACCT GTGTTTTAGC TGTTGGACAA ACATCAGCCC TATTTACCCA 
GCTATGAGCA AAACAAAGGC AACCTCCCGA CTTCTAGTAA ATTACCCAGA GCCCTACCGT 
TCTGAAATAC TGGATTATCT CTTTAAGCCA AACTTCGGTG CCTCATTGCA TATTCTAAAA 
GTTGAAATAG GTGGTGATGG GCAGACAACA GATGGCACAG AGCCCTCCCA CATGCACTAT 
GCACTGGATG AGAATTATTT CCGAGGGTAT GAATGGTGGT TGATGAAGGA AGCTAAGAAG 
CGGAACCCTA ATATTACACT CATAGGGCTG CCATGGTCGT TCCCTGGATG GCTGGGGAAG 
GGCTTCAATT GGCCTTATGT CAATCTCCAG CTGACCGCCT ACTACATCGT GACCTGGATT 
GTGGGCTCCA AGCATTATCA TGACCTGGAC ATTGATTATA TCGGAATTTG GAATGAGAGG 
GCATTTGACA TTAATTATAT CAAGGTGTTA AGAAGAATGC TGAACCATCA AGGTCTCCAG 
CACGTGAAAA TCATAGCGAG TGATAATCTC TGGGAGCCCA TTTCTGCTTC TATGCTGCTT 
GACCCTGAGC TCTTGAAGGT GATTGATGTA ATAGGAGCTC ATTACCCTGG GACACTTACA 
GTAAAGGATG CTAGGTTGAC TAAGAAGAAG CTTTGGTCTT CTGAAGATTT TAGCACTGTA 
AATGGTGATA TGGGTGCAGG CTGCTGGGGT CGCATATTGA ATCAGAATTA CGTCAATGGC 
TACATGACTT CCACCATTGC TTGGAACTTG GTGGCCAGTT ACTATCAGCA GTTGCCATAT 
GGGCGTTGTG GACTGATGAC TGCTCAGGAG CCATGGAGTG GGCACTACGT GGTGGAAGCT 
CCTATCTGGG TATCAGCTCA TACTACTCAG TTTACTCAAC CAGGTTGGTA TTACCTGAAG 
ACAGTTGGCC ATTTAGAGAA AGGAGGAAGC TACGTAGCTC TGACGGATGG CTTAGGTAAC 
CTCACTGTCA TCGTTGAAAC TATGAGTTAT AAACATTCCA AGTGTATACG ACCACTTCTT 
CCCTATTTCA ATGTGTCACA TCAGTTTGCA ACGTTTGATC TTAAGGGTTC TTTTAGTCAA 
ATACCAGAGC TACAGGTGTG GTATACCAAA CTCGGAAAAC CATCGGAGAG ATTTCTCTTC 
AAACAACTGG ATTCTCTATG GCTGCTCGAC AGCGGTGGCA GCTTCACGCT GGAGCTGCAG 
GAGGACGAGC TGTTCACCCT CACCACGCTC ACCTCCGGCC GCAAAGGCAG CTTCCCGCCG 
CCTCCCAAGT CCCAGCGCTT CCCCTCTGTC TATGAGGATA ACTTCAACAT TGATTACCCG 
TTTTTTAGTG AAGCTCCGAA TTTTGCTGAC CAGACTGGGG TGTTTGAATA CTACGTGAAT 
ATGGGAGACC CCGGAGACCA TCGCTTCACT CTGCGGCAGG TTCTCAATCA GAGGCCCATC 
ACGTGGGCTG CCGACGCGTT CAACACCATC AGCGTTATCG GGGATTACAC GTGGTCCAAT 
CTGACTATAA AGTGTGATGT GTACATAGAG ACCCCTGAGA AGGGAGGCGT GTTCATTGCT 
GGAAGAGTCA ATAAAGGTGG TATTTTGATT AGAAGTGCCA GGGGGATTTT CTTCTGGATT 
TTTGCAAATG GAACCTACAG AGTCACAGGC GATCTAGCTG GATGGATCAT ATATAGTTTA 
GGACATGCTG ATGTTACAGC AAAAAAATGG TATACACTCA CTTTAACTCT GAATGGTCGT 
TTCTCCTCTG GCAAGCTGAA TGGCAAGACC CTCTGGAAGA ACATCTCTGT GAACTTCCCA 
GAAAACGGCT GGGCCGCGAT TGGGACTCAC TCCTTTGAAT TTGCGCAATT TGACAACTTT 
CGTGTGGAAG CCTCACACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GALC ( XM_006061465.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006061527.2 Bubalus bubalis galactosylceramidase (GALC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu44408
Clone ID Related Accession (Same CDS sequence)	XM_006061465.2
Accession Version	XM_006061465.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	galactocerebrosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006061465.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTGTC ACACAATGGC TGAGCGCCTA ATTCAGGCCT CCTGGCAACG CCGGGCGAAA 
GCCATGACGG CCGCCGCGGG TTTGGCGGGC CAAGCCGCGG TGCCCCTGCT GCTGTGCGCG 
CTGCTGGCAC CCAGCGGCGC GTACGTGCTC GACGACTCCG AGGGGCTGGG CCGGGAGTTC 
GACGGCATCG GAGCTGTCAG CGGCGGCGGG GCAACCTCCC GACTTCTAGT AAATTACCCA 
GAGCCCTACC GTTCTGAAAT ACTGGATTAT CTCTTTAAGC CAAACTTCGG TGCCTCATTG 
CATATTCTAA AAGTTGAAAT AGGTGGTGAT GGGCAGACAA CAGATGGCAC AGAGCCCTCC 
CACATGCACT ATGCACTGGA TGAGAATTAT TTCCGAGGGT ATGAATGGTG GTTGATGAAG 
GAAGCTAAGA AGCGGAACCC TAATATTACA CTCATAGGGC TGCCATGGTC GTTCCCTGGA 
TGGCTGGGGA AGGGCTTCAA TTGGCCTTAT GTCAATCTCC AGCTGACCGC CTACTACATC 
GTGACCTGGA TTGTGGGCTC CAAGCATTAT CATGACCTGG ACATTGATTA TATCGGAATT 
TGGAATGAGA GGGCATTTGA CATTAATTAT ATCAAGGTGT TAAGAAGAAT GCTGAACCAT 
CAAGGTCTCC AGCACGTGAA AATCATAGCG AGTGATAATC TCTGGGAGCC CATTTCTGCT 
TCTATGCTGC TTGACCCTGA GCTCTTGAAG GTGATTGATG TAATAGGAGC TCATTACCCT 
GGGACACTTA CAGTAAAGGA TGCTAGGTTG ACTAAGAAGA AGCTTTGGTC TTCTGAAGAT 
TTTAGCACTG TAAATGGTGA TATGGGTGCA GGCTGCTGGG GTCGCATATT GAATCAGAAT 
TACGTCAATG GCTACATGAC TTCCACCATT GCTTGGAACT TGGTGGCCAG TTACTATCAG 
CAGTTGCCAT ATGGGCGTTG TGGACTGATG ACTGCTCAGG AGCCATGGAG TGGGCACTAC 
GTGGTGGAAG CTCCTATCTG GGTATCAGCT CATACTACTC AGTTTACTCA ACCAGGTTGG 
TATTACCTGA AGACAGTTGG CCATTTAGAG AAAGGAGGAA GCTACGTAGC TCTGACGGAT 
GGCTTAGGTA ACCTCACTGT CATCGTTGAA ACTATGAGTT ATAAACATTC CAAGTGTATA 
CGACCACTTC TTCCCTATTT CAATGTGTCA CATCAGTTTG CAACGTTTGA TCTTAAGGGT 
TCTTTTAGTC AAATACCAGA GCTACAGGTG TGGTATACCA AACTCGGAAA ACCATCGGAG 
AGATTTCTCT TCAAACAACT GGATTCTCTA TGGCTGCTCG ACAGCGGTGG CAGCTTCACG 
CTGGAGCTGC AGGAGGACGA GCTGTTCACC CTCACCACGC TCACCTCCGG CCGCAAAGGC 
AGCTTCCCGC CGCCTCCCAA GTCCCAGCGC TTCCCCTCTG TCTATGAGGA TAACTTCAAC 
ATTGATTACC CGTTTTTTAG TGAAGCTCCG AATTTTGCTG ACCAGACTGG GGTGTTTGAA 
TACTACGTGA ATATGGGAGA CCCCGGAGAC CATCGCTTCA CTCTGCGGCA GGTTCTCAAT 
CAGAGGCCCA TCACGTGGGC TGCCGACGCG TTCAACACCA TCAGCGTTAT CGGGGATTAC 
ACGTGGTCCA ATCTGACTAT AAAGTGTGAT GTGTACATAG AGACCCCTGA GAAGGGAGGC 
GTGTTCATTG CTGGAAGAGT CAATAAAGGT GGTATTTTGA TTAGAAGTGC CAGGGGGATT 
TTCTTCTGGA TTTTTGCAAA TGGAACCTAC AGAGTCACAG GCGATCTAGC TGGATGGATC 
ATATATAGTT TAGGACATGC TGATGTTACA GCAAAAAAAT GGTATACACT CACTTTAACT 
CTGAATGGTC GTTTCTCCTC TGGCAAGCTG AATGGCAAGA CCCTCTGGAA GAACATCTCT 
GTGAACTTCC CAGAAAACGG CTGGGCCGCG ATTGGGACTC ACTCCTTTGA ATTTGCGCAA 
TTTGACAACT TTCGTGTGGA AGCCTCACAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006061527.2
CDS	59..2131
Translation	MTCHTMAERLIQASWQRRAKAMTAAAGLAGQAAVPLLLCALLAPSGAYVLDDSEGLGREFDGIGAVSGGGATSRLLVNYPEPYRSEILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFNWPYVNLQLTAYYIVTWIVGSKHYHDLDIDYIGIWNERAFDINYIKVLRRMLNHQGLQHVKIIASDNLWEPISASMLLDPELLKVIDVIGAHYPGTLTVKDARLTKKKLWSSEDFSTVNGDMGAGCWGRILNQNYVNGYMTSTIAWNLVASYYQQLPYGRCGLMTAQEPWSGHYVVEAPIWVSAHTTQFTQPGWYYLKTVGHLEKGGSYVALTDGLGNLTVIVETMSYKHSKCIRPLLPYFNVSHQFATFDLKGSFSQIPELQVWYTKLGKPSERFLFKQLDSLWLLDSGGSFTLELQEDELFTLTTLTSGRKGSFPPPPKSQRFPSVYEDNFNIDYPFFSEAPNFADQTGVFEYYVNMGDPGDHRFTLRQVLNQRPITWAADAFNTISVIGDYTWSNLTIKCDVYIETPEKGGVFIAGRVNKGGILIRSARGIFFWIFANGTYRVTGDLAGWIIYSLGHADVTAKKWYTLTLTLNGRFSSGKLNGKTLWKNISVNFPENGWAAIGTHSFEFAQFDNFRVEASH

Target ORF information:

RefSeq Version	XM_006061465.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis galactosylceramidase (GALC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006061465.2

ORF Insert Sequence:

ATGACCTGTC ACACAATGGC TGAGCGCCTA ATTCAGGCCT CCTGGCAACG CCGGGCGAAA 
GCCATGACGG CCGCCGCGGG TTTGGCGGGC CAAGCCGCGG TGCCCCTGCT GCTGTGCGCG 
CTGCTGGCAC CCAGCGGCGC GTACGTGCTC GACGACTCCG AGGGGCTGGG CCGGGAGTTC 
GACGGCATCG GAGCTGTCAG CGGCGGCGGG GCAACCTCCC GACTTCTAGT AAATTACCCA 
GAGCCCTACC GTTCTGAAAT ACTGGATTAT CTCTTTAAGC CAAACTTCGG TGCCTCATTG 
CATATTCTAA AAGTTGAAAT AGGTGGTGAT GGGCAGACAA CAGATGGCAC AGAGCCCTCC 
CACATGCACT ATGCACTGGA TGAGAATTAT TTCCGAGGGT ATGAATGGTG GTTGATGAAG 
GAAGCTAAGA AGCGGAACCC TAATATTACA CTCATAGGGC TGCCATGGTC GTTCCCTGGA 
TGGCTGGGGA AGGGCTTCAA TTGGCCTTAT GTCAATCTCC AGCTGACCGC CTACTACATC 
GTGACCTGGA TTGTGGGCTC CAAGCATTAT CATGACCTGG ACATTGATTA TATCGGAATT 
TGGAATGAGA GGGCATTTGA CATTAATTAT ATCAAGGTGT TAAGAAGAAT GCTGAACCAT 
CAAGGTCTCC AGCACGTGAA AATCATAGCG AGTGATAATC TCTGGGAGCC CATTTCTGCT 
TCTATGCTGC TTGACCCTGA GCTCTTGAAG GTGATTGATG TAATAGGAGC TCATTACCCT 
GGGACACTTA CAGTAAAGGA TGCTAGGTTG ACTAAGAAGA AGCTTTGGTC TTCTGAAGAT 
TTTAGCACTG TAAATGGTGA TATGGGTGCA GGCTGCTGGG GTCGCATATT GAATCAGAAT 
TACGTCAATG GCTACATGAC TTCCACCATT GCTTGGAACT TGGTGGCCAG TTACTATCAG 
CAGTTGCCAT ATGGGCGTTG TGGACTGATG ACTGCTCAGG AGCCATGGAG TGGGCACTAC 
GTGGTGGAAG CTCCTATCTG GGTATCAGCT CATACTACTC AGTTTACTCA ACCAGGTTGG 
TATTACCTGA AGACAGTTGG CCATTTAGAG AAAGGAGGAA GCTACGTAGC TCTGACGGAT 
GGCTTAGGTA ACCTCACTGT CATCGTTGAA ACTATGAGTT ATAAACATTC CAAGTGTATA 
CGACCACTTC TTCCCTATTT CAATGTGTCA CATCAGTTTG CAACGTTTGA TCTTAAGGGT 
TCTTTTAGTC AAATACCAGA GCTACAGGTG TGGTATACCA AACTCGGAAA ACCATCGGAG 
AGATTTCTCT TCAAACAACT GGATTCTCTA TGGCTGCTCG ACAGCGGTGG CAGCTTCACG 
CTGGAGCTGC AGGAGGACGA GCTGTTCACC CTCACCACGC TCACCTCCGG CCGCAAAGGC 
AGCTTCCCGC CGCCTCCCAA GTCCCAGCGC TTCCCCTCTG TCTATGAGGA TAACTTCAAC 
ATTGATTACC CGTTTTTTAG TGAAGCTCCG AATTTTGCTG ACCAGACTGG GGTGTTTGAA 
TACTACGTGA ATATGGGAGA CCCCGGAGAC CATCGCTTCA CTCTGCGGCA GGTTCTCAAT 
CAGAGGCCCA TCACGTGGGC TGCCGACGCG TTCAACACCA TCAGCGTTAT CGGGGATTAC 
ACGTGGTCCA ATCTGACTAT AAAGTGTGAT GTGTACATAG AGACCCCTGA GAAGGGAGGC 
GTGTTCATTG CTGGAAGAGT CAATAAAGGT GGTATTTTGA TTAGAAGTGC CAGGGGGATT 
TTCTTCTGGA TTTTTGCAAA TGGAACCTAC AGAGTCACAG GCGATCTAGC TGGATGGATC 
ATATATAGTT TAGGACATGC TGATGTTACA GCAAAAAAAT GGTATACACT CACTTTAACT 
CTGAATGGTC GTTTCTCCTC TGGCAAGCTG AATGGCAAGA CCCTCTGGAA GAACATCTCT 
GTGAACTTCC CAGAAAACGG CTGGGCCGCG ATTGGGACTC ACTCCTTTGA ATTTGCGCAA 
TTTGACAACT TTCGTGTGGA AGCCTCACAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GALC ( XM_025295139.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025150924.1 Bubalus bubalis galactosylceramidase (GALC), transcript variant X2, mRNA.

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CloneID	OBu44409
Clone ID Related Accession (Same CDS sequence)	XM_025295139.1
Accession Version	XM_025295139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	galactocerebrosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTGTC ACACAATGGC TGAGCGCCTA ATTCAGGCCT CCTGGCAACG CCGGGCGAAA 
GCCATGACGG CCGCCGCGGG TTTGGCGGGC CAAGCCGCGG TGCCCCTGCT GCTGTGCGCG 
CTGCTGGCAC CCAGCGGCGC GTACGTGCTC GACGACTCCG AGGGGCTGGG CCGGGAGTTC 
GACGGCATCG GAGCTGTCAG CGGCGGCGGG GCAACCTCCC GACTTCTAGT AAATTACCCA 
GAGCCCTACC GTTCTGAAAT ACTGGATTAT CTCTTTAAGC CAAACTTCGG TGCCTCATTG 
CATATTCTAA AAGTTGAAAT AGGTGGTGAT GGGCAGACAA CAGATGGCAC AGAGCCCTCC 
CACATGCACT ATGCACTGGA TGAGAATTAT TTCCGAGGGT ATGAATGGTG GTTGATGAAG 
GAAGCTAAGA AGCGGAACCC TAATATTACA CTCATAGGGC TGCCATGGTC GTTCCCTGGA 
TGGCTGGGGA AGGGCTTCAA TTGGCCTTAT GTCAATCTCC AGCTGACCGC CTACTACATC 
GTGACCTGGA TTGTGGGCTC CAAGCATTAT CATGACCTGG ACATTGATTA TATCGGAGTG 
TTAAGAAGAA TGCTGAACCA TCAAGGTCTC CAGCACGTGA AAATCATAGC GAGTGATAAT 
CTCTGGGAGC CCATTTCTGC TTCTATGCTG CTTGACCCTG AGCTCTTGAA GGTGATTGAT 
GTAATAGGAG CTCATTACCC TGGGACACTT ACAGTAAAGG ATGCTAGGTT GACTAAGAAG 
AAGCTTTGGT CTTCTGAAGA TTTTAGCACT GTAAATGGTG ATATGGGTGC AGGCTGCTGG 
GGTCGCATAT TGAATCAGAA TTACGTCAAT GGCTACATGA CTTCCACCAT TGCTTGGAAC 
TTGGTGGCCA GTTACTATCA GCAGTTGCCA TATGGGCGTT GTGGACTGAT GACTGCTCAG 
GAGCCATGGA GTGGGCACTA CGTGGTGGAA GCTCCTATCT GGGTATCAGC TCATACTACT 
CAGTTTACTC AACCAGGTTG GTATTACCTG AAGACAGTTG GCCATTTAGA GAAAGGAGGA 
AGCTACGTAG CTCTGACGGA TGGCTTAGGT AACCTCACTG TCATCGTTGA AACTATGAGT 
TATAAACATT CCAAGTGTAT ACGACCACTT CTTCCCTATT TCAATGTGTC ACATCAGTTT 
GCAACGTTTG ATCTTAAGGG TTCTTTTAGT CAAATACCAG AGCTACAGGT GTGGTATACC 
AAACTCGGAA AACCATCGGA GAGATTTCTC TTCAAACAAC TGGATTCTCT ATGGCTGCTC 
GACAGCGGTG GCAGCTTCAC GCTGGAGCTG CAGGAGGACG AGCTGTTCAC CCTCACCACG 
CTCACCTCCG GCCGCAAAGG CAGCTTCCCG CCGCCTCCCA AGTCCCAGCG CTTCCCCTCT 
GTCTATGAGG ATAACTTCAA CATTGATTAC CCGTTTTTTA GTGAAGCTCC GAATTTTGCT 
GACCAGACTG GGGTGTTTGA ATACTACGTG AATATGGGAG ACCCCGGAGA CCATCGCTTC 
ACTCTGCGGC AGGTTCTCAA TCAGAGGCCC ATCACGTGGG CTGCCGACGC GTTCAACACC 
ATCAGCGTTA TCGGGGATTA CACGTGGTCC AATCTGACTA TAAAGTGTGA TGTGTACATA 
GAGACCCCTG AGAAGGGAGG CGTGTTCATT GCTGGAAGAG TCAATAAAGG TGGTATTTTG 
ATTAGAAGTG CCAGGGGGAT TTTCTTCTGG ATTTTTGCAA ATGGAACCTA CAGAGTCACA 
GGCGATCTAG CTGGATGGAT CATATATAGT TTAGGACATG CTGATGTTAC AGCAAAAAAA 
TGGTATACAC TCACTTTAAC TCTGAATGGT CGTTTCTCCT CTGGCAAGCT GAATGGCAAG 
ACCCTCTGGA AGAACATCTC TGTGAACTTC CCAGAAAACG GCTGGGCCGC GATTGGGACT 
CACTCCTTTG AATTTGCGCA ATTTGACAAC TTTCGTGTGG AAGCCTCACA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025150924.1
CDS	61..2094
Translation	MTCHTMAERLIQASWQRRAKAMTAAAGLAGQAAVPLLLCALLAPSGAYVLDDSEGLGREFDGIGAVSGGGATSRLLVNYPEPYRSEILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYALDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFNWPYVNLQLTAYYIVTWIVGSKHYHDLDIDYIGVLRRMLNHQGLQHVKIIASDNLWEPISASMLLDPELLKVIDVIGAHYPGTLTVKDARLTKKKLWSSEDFSTVNGDMGAGCWGRILNQNYVNGYMTSTIAWNLVASYYQQLPYGRCGLMTAQEPWSGHYVVEAPIWVSAHTTQFTQPGWYYLKTVGHLEKGGSYVALTDGLGNLTVIVETMSYKHSKCIRPLLPYFNVSHQFATFDLKGSFSQIPELQVWYTKLGKPSERFLFKQLDSLWLLDSGGSFTLELQEDELFTLTTLTSGRKGSFPPPPKSQRFPSVYEDNFNIDYPFFSEAPNFADQTGVFEYYVNMGDPGDHRFTLRQVLNQRPITWAADAFNTISVIGDYTWSNLTIKCDVYIETPEKGGVFIAGRVNKGGILIRSARGIFFWIFANGTYRVTGDLAGWIIYSLGHADVTAKKWYTLTLTLNGRFSSGKLNGKTLWKNISVNFPENGWAAIGTHSFEFAQFDNFRVEASH

Target ORF information:

RefSeq Version	XM_025295139.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis galactosylceramidase (GALC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025295139.1

ORF Insert Sequence:

ATGACCTGTC ACACAATGGC TGAGCGCCTA ATTCAGGCCT CCTGGCAACG CCGGGCGAAA 
GCCATGACGG CCGCCGCGGG TTTGGCGGGC CAAGCCGCGG TGCCCCTGCT GCTGTGCGCG 
CTGCTGGCAC CCAGCGGCGC GTACGTGCTC GACGACTCCG AGGGGCTGGG CCGGGAGTTC 
GACGGCATCG GAGCTGTCAG CGGCGGCGGG GCAACCTCCC GACTTCTAGT AAATTACCCA 
GAGCCCTACC GTTCTGAAAT ACTGGATTAT CTCTTTAAGC CAAACTTCGG TGCCTCATTG 
CATATTCTAA AAGTTGAAAT AGGTGGTGAT GGGCAGACAA CAGATGGCAC AGAGCCCTCC 
CACATGCACT ATGCACTGGA TGAGAATTAT TTCCGAGGGT ATGAATGGTG GTTGATGAAG 
GAAGCTAAGA AGCGGAACCC TAATATTACA CTCATAGGGC TGCCATGGTC GTTCCCTGGA 
TGGCTGGGGA AGGGCTTCAA TTGGCCTTAT GTCAATCTCC AGCTGACCGC CTACTACATC 
GTGACCTGGA TTGTGGGCTC CAAGCATTAT CATGACCTGG ACATTGATTA TATCGGAGTG 
TTAAGAAGAA TGCTGAACCA TCAAGGTCTC CAGCACGTGA AAATCATAGC GAGTGATAAT 
CTCTGGGAGC CCATTTCTGC TTCTATGCTG CTTGACCCTG AGCTCTTGAA GGTGATTGAT 
GTAATAGGAG CTCATTACCC TGGGACACTT ACAGTAAAGG ATGCTAGGTT GACTAAGAAG 
AAGCTTTGGT CTTCTGAAGA TTTTAGCACT GTAAATGGTG ATATGGGTGC AGGCTGCTGG 
GGTCGCATAT TGAATCAGAA TTACGTCAAT GGCTACATGA CTTCCACCAT TGCTTGGAAC 
TTGGTGGCCA GTTACTATCA GCAGTTGCCA TATGGGCGTT GTGGACTGAT GACTGCTCAG 
GAGCCATGGA GTGGGCACTA CGTGGTGGAA GCTCCTATCT GGGTATCAGC TCATACTACT 
CAGTTTACTC AACCAGGTTG GTATTACCTG AAGACAGTTG GCCATTTAGA GAAAGGAGGA 
AGCTACGTAG CTCTGACGGA TGGCTTAGGT AACCTCACTG TCATCGTTGA AACTATGAGT 
TATAAACATT CCAAGTGTAT ACGACCACTT CTTCCCTATT TCAATGTGTC ACATCAGTTT 
GCAACGTTTG ATCTTAAGGG TTCTTTTAGT CAAATACCAG AGCTACAGGT GTGGTATACC 
AAACTCGGAA AACCATCGGA GAGATTTCTC TTCAAACAAC TGGATTCTCT ATGGCTGCTC 
GACAGCGGTG GCAGCTTCAC GCTGGAGCTG CAGGAGGACG AGCTGTTCAC CCTCACCACG 
CTCACCTCCG GCCGCAAAGG CAGCTTCCCG CCGCCTCCCA AGTCCCAGCG CTTCCCCTCT 
GTCTATGAGG ATAACTTCAA CATTGATTAC CCGTTTTTTA GTGAAGCTCC GAATTTTGCT 
GACCAGACTG GGGTGTTTGA ATACTACGTG AATATGGGAG ACCCCGGAGA CCATCGCTTC 
ACTCTGCGGC AGGTTCTCAA TCAGAGGCCC ATCACGTGGG CTGCCGACGC GTTCAACACC 
ATCAGCGTTA TCGGGGATTA CACGTGGTCC AATCTGACTA TAAAGTGTGA TGTGTACATA 
GAGACCCCTG AGAAGGGAGG CGTGTTCATT GCTGGAAGAG TCAATAAAGG TGGTATTTTG 
ATTAGAAGTG CCAGGGGGAT TTTCTTCTGG ATTTTTGCAA ATGGAACCTA CAGAGTCACA 
GGCGATCTAG CTGGATGGAT CATATATAGT TTAGGACATG CTGATGTTAC AGCAAAAAAA 
TGGTATACAC TCACTTTAAC TCTGAATGGT CGTTTCTCCT CTGGCAAGCT GAATGGCAAG 
ACCCTCTGGA AGAACATCTC TGTGAACTTC CCAGAAAACG GCTGGGCCGC GATTGGGACT 
CACTCCTTTG AATTTGCGCA ATTTGACAAC TTTCGTGTGG AAGCCTCACA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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GALC cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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