ZNF521 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZNF521
Entrez Gene ID	102409668
Full Name	zinc finger protein 521
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006045477.1	XP_006045539.1	zinc finger protein 521-like isoform X2
XM_025273509.1	XP_025129294.1	zinc finger protein 521 isoform X1
XM_006045476.1	XP_006045538.1	zinc finger protein 521-like isoform X1
XM_025273510.1	XP_025129295.1	zinc finger protein 521 isoform X3
XM_025273508.1	XP_025129293.1	zinc finger protein 521 isoform X1
XM_006045476.2	XP_006045538.1	zinc finger protein 521 isoform X2
XM_025273511.1	XP_025129296.1	zinc finger protein 521 isoform X4

Pathways from BioSystems
Gene Ontology

The following ZNF521 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZNF521 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu04494	XM_006045476.1 Latest version!	Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu04495	XM_006045477.1 Latest version!	Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu58660	XM_025273509.1 Latest version!	Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu58660	XM_025273508.1 Latest version!	Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu58661	XM_006045476.2 Latest version!	Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu58662	XM_025273510.1 Latest version!	Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu58663	XM_025273511.1 Latest version!	Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZNF521 ( XM_006045476.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006045538.1 Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu04494
Clone ID Related Accession (Same CDS sequence)	XM_006045476.1
Accession Version	XM_006045476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	zinc finger protein 521-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783729.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACATTT GGCAAGGTTG TCATTCTGTA AGAAAAGACC CCAACTGTAA ACTTGAAGAC 
AAGACTGAAG ATGGAGAAGC AATAGACTGT AAGAAGAGGC CGGAAGACGG GGAGGAGCTA 
GAGGACGAAG CTGTGCACAG CTGTGAGAGC TGCCTCCAGG TGTTCGAGTC ACTGAGCGAT 
ATCACAGAAC ACAAGATCAA TCAATGTCAG CTGACAGATG GAGTGGATGT AGAGGATGAC 
CCCACTTGCT CCTGGCCAGC TTCCTCACCT TCGAGCAAGG ACCAGACTTC CCCCAGCCAC 
GGAGAAGGTT GCGATTTTGG AGAGGAAGAA GGTGGCCCCG GACTCCCGTA CCCGTGCCAG 
TTCTGTGACA AGTCATTCAG CCGCCTCAGC TACCTAAAGC ACCATGAGCA GAGTCACAGC 
GACAAGCTGC CTTTCAAGTG CACCTACTGC AGCCGGCTGT TCAAACACAA GCGGAGCCGA 
GACCGCCACA TCAAACTCCA CACCGGGGAC AAGAAGTACC ACTGCAGTGA GTGCGACGCC 
GCCTTCTCCA GAAGTGATCA CCTGAAGATC CACTTGAAGA CTCACACGTC CAACAAGCCA 
TATAAATGTG CCATTTGTCG CCGTGGGTTC CTGTCCTCGA GTTCCTTACA CGGGCACATG 
CAGGTCCACG AGAGGAGCAA GGACGGGTCC CAGTCGGGTT CCAGGATGGA GGACTGGAAG 
ATGAAGGACA CTCAGAAGTG CAGTCAGTGT GAGGAAGGCT TTGACTTCCC GGAAGACCTC 
CAAAAGCACA TCGCCGAGTG CCACCCCGAG TGCTCCCCGA ACGAGGACCG GGCGGCCCTC 
CAGTGCATCT ACTGCCACGA GCTCTTCGTG GAGGAGACCT CCCTGATGAA CCACATGGAG 
CAGATGCACG GCGGGGAGAA GAAGAACTCC TGCAGCATCT GTTCCGAGAG CTTCCACAGC 
GTCGAGGAGC TGTACAGCCA CATGGACAGT CACCAGCAAC CGGAGTCGTG CAACCACAGC 
AACAGCCCTT CCCTGGTCAC GGTGGGCTAC ACCTCCGTGT CCAGCACGAC CCCGGACTCC 
AACCTCTCGG TGGACAGCTC GACCATGGTG GAAGCCGCCC CACCCATCCC AAAGAGTCGG 
GGGAGGAAGC GGGCCGCTCA GCAGACCCCG GACATGACTG TCCCCTCGAG TAAACAGGCG 
AAGGTCACCT ACAGCTGTAT TTACTGCAAC AAGCAGTTGT TTTCCAGTCT GGCGGTTCTG 
CAGATTCACC TGAAAACTAT GCATTTAGAT AAGCCAGAGC AGGCCCACAT CTGTCAGTAC 
TGCTTGGAGG TACTGCCCTC CCTCTATAAC CTCAATGAAC ATCTTAAGCA AGTGCATGAA 
GCTCAGGACC CAGGTCTGAT CGTTTCTGCC TTGCCCGCCA TAGTCTACCA GTGCAACTTC 
TGCTCTGAAG TTGTCAACGA CCTCAACACC CTTCAGGAAC ACATCCGATG TTCTCACGGA 
TTTGCGAACC CCGCGGCAAA GGACAGCAAT GCTTTCTTCT GTCCCCACTG CTACATGGGG 
TTCCTCACTG ACTCTTCGCT GGAAGAGCAT ATAAGACAGG TCCATTGTGA CCTCAGTGGC 
TCCCGATTTG GGTCCCCAGT GCTCGGGACT CCGAAAGAGC CAGTCGTAGA AGTCTATTCT 
TGTTCCTATT GTACAAATTC CCCCATATTC AACAGCGTTC TTAAACTGAA CAAGCACATC 
AAAGAGAATC ATAAAAACAT TCCCTTGGCC CTGAATTATA TCCACAACGG GAAGAAATCC 
AGGGCCTTGA GTCCCCTGTC ACCTGTGGCC ATAGAGCAGA CATCTCTGAA GATGATGCAG 
GCGGTAGGAG GGGCGCCTGC AAGGCAGGCT GGAGAATATA TCTGTAATCA GTGTGGTGCT 
AAGTACACGT CCCTGGACGG CTTTCAGACT CACCTGAAGA CCCACCTGGA CACCGTGCTC 
CCGAAACTGA CCTGTCCTCA GTGCAACAAG GAGTTTCCCA ACCAAGAGTC CCTGCTGAAG 
CATGTGACCA TTCATTTCAT GATCACCTCC ACGTACTACA TCTGCGAGAG CTGTGACAAG 
CAGTTCACAT CGGTGGACGA CCTGCAGAAA CACCTGCTGG ACATGCACAC CTTCGTCTTC 
TTCCGCTGCA CCCTCTGCCA GGAGGTGTTC GACTCCAAGG TCTCCATCCA ACTCCACCTG 
GCCGTGAAGC ACAGTAATGA GAAGAAAGTA TATCGCTGCA CCTCCTGCAA CTGGGACTTC 
CGCAACGAGA CGGACCTGCA GCTGCACGTG AAGCACAACC ACCTGGAGAA CCAGGGCAAA 
GTGCACAAGT GCATCTTCTG CGGCGAGTCC TTCGGCACTG AGGTGGAGCT GCAGTGTCAC 
ATCACCACGC ACAGCAAGAA GTACAACTGC CAGTTCTGCA GCAAGGCCTT CCATGCCATC 
ATCCTGCTGG AGAAGCACCT GCGGGAGAAG CACTGTGTGT TCGACACCAA GACGCCCAAC 
TGCGGCGCCA ACGGGGCCTC GGAGCCGGGC CAGAAGGAGG AGGTGGAGTT GCAGACCCTG 
CTGACCAACA GCCAGGAGTC CCACAACAGC CACGACGGCA GCGAGGAGGA CGTGGACACC 
TCGGAGCCCA TGTACGGCTG CGACATCTGC GGGGCGGCCT ACACCATGGA GACGCTCCTG 
CAGAACCACC AACTCCGGGA CCACAACATC CGGCCGGGGG AGAGCGCCAT TGTCAAGAAG 
AAAGCCGAGC TCATCAAAGG GAACTACAAG TGCAACGTGT GCTCGCGGAC CTTCTTCTCG 
GAGAACGGCC TCCGGGAGCA CATGCAGACG CACCTGGGCC CCGTGAAACA TTACATGTGC 
CCCATCTGTG GGGAGCGCTT CCCCTCGCTC TTGACCTTGA CCGAACACAA GGTCACGCAC 
AGTAAGAGCC TGGATACAGG AAACTGCCGC ATCTGCAAGA TGCCATTGCA GAGCGAGGAG 
GAGTTCCTGG AGCATTGCCA GATGCACCCC GACCTGCGGA ACTCCCTGAC GGGGTTCCGC 
TGCGTGGTTT GCATGCAGAC GGTGACCTCC ACCCTGGAGC TCAAAATCCA CGGGACGTTC 
CACATGCAGA AGACGGGCAA CGGGGCCACG GTCCAGGCCA CTGGCCGCGG CCCACACGTC 
CCCAAACTGT ACAAGTGTGC GTCCTGTCTC AAAGAATTCC GTTCCAAGCA AGATCTGGTG 
AAACTGGACA TCAACGGCCT GCCCTACGGT CTGTGCGCCG GCTGCGTGAA CCTCAGTAAG 
AGCGCCAGTC CCGGGGTCAA CATCGCTCCC GGCTCCAGTC GACCGGGCCT GGGCCAGACC 
GAGAACCTGG GGGCCGTGGA GGGCAAGGGC AAGGCCGGCG CCCCCAAGAC GCGCTGCTCC 
AGCTGCAACG TGAAGTTTGA GTCCGAAAGT GAACTCCAGA ACCACATTCA AACGGTCCAC 
CGAGAGCTGG TGCCCGACAG CAACAGCACG CAGTTGAAAA CGCCTCAAGT CTCGCCCATG 
CCCAGGATCA GCCCCTCGCA ATCGGATGAG AAGAAGACCT ATCAGTGCAT CAAGTGTCAG 
ATGGTTTTCT ACAATGAATG GGATATTCAG GTGCATGTTG CAAATCACAT GATTGATGAA 
GGGCTGAACC ACGAGTGCAA ACTTTGCAGC CAAACCTTCG ACTCCCCTGC CAAGCTCCAG 
TGCCACCTGA TCGAGCACAG CTTCGAAGGG ATGGGCGGTA CCTTCAAGTG TCCCGTCTGC 
TTTACAGTGT TTGTTCAAGC AAACAAGTTG CAGCAGCATA TTTTCTCTGC CCATGGACAA 
GAAGACAAGA TCTACGACTG CACACAATGT CCACAGAAGT TTTTCTTCCA AACAGAGCTG 
CAGAACCACA CGATGACCCA ACACAGCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006045538.1
CDS	12..3944
Translation	MHIWQGCHSVRKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCESCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERSKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCIYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKAGAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_006045476.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006045476.1

ORF Insert Sequence:

ATGCACATTT GGCAAGGTTG TCATTCTGTA AGAAAAGACC CCAACTGTAA ACTTGAAGAC 
AAGACTGAAG ATGGAGAAGC AATAGACTGT AAGAAGAGGC CGGAAGACGG GGAGGAGCTA 
GAGGACGAAG CTGTGCACAG CTGTGAGAGC TGCCTCCAGG TGTTCGAGTC ACTGAGCGAT 
ATCACAGAAC ACAAGATCAA TCAATGTCAG CTGACAGATG GAGTGGATGT AGAGGATGAC 
CCCACTTGCT CCTGGCCAGC TTCCTCACCT TCGAGCAAGG ACCAGACTTC CCCCAGCCAC 
GGAGAAGGTT GCGATTTTGG AGAGGAAGAA GGTGGCCCCG GACTCCCGTA CCCGTGCCAG 
TTCTGTGACA AGTCATTCAG CCGCCTCAGC TACCTAAAGC ACCATGAGCA GAGTCACAGC 
GACAAGCTGC CTTTCAAGTG CACCTACTGC AGCCGGCTGT TCAAACACAA GCGGAGCCGA 
GACCGCCACA TCAAACTCCA CACCGGGGAC AAGAAGTACC ACTGCAGTGA GTGCGACGCC 
GCCTTCTCCA GAAGTGATCA CCTGAAGATC CACTTGAAGA CTCACACGTC CAACAAGCCA 
TATAAATGTG CCATTTGTCG CCGTGGGTTC CTGTCCTCGA GTTCCTTACA CGGGCACATG 
CAGGTCCACG AGAGGAGCAA GGACGGGTCC CAGTCGGGTT CCAGGATGGA GGACTGGAAG 
ATGAAGGACA CTCAGAAGTG CAGTCAGTGT GAGGAAGGCT TTGACTTCCC GGAAGACCTC 
CAAAAGCACA TCGCCGAGTG CCACCCCGAG TGCTCCCCGA ACGAGGACCG GGCGGCCCTC 
CAGTGCATCT ACTGCCACGA GCTCTTCGTG GAGGAGACCT CCCTGATGAA CCACATGGAG 
CAGATGCACG GCGGGGAGAA GAAGAACTCC TGCAGCATCT GTTCCGAGAG CTTCCACAGC 
GTCGAGGAGC TGTACAGCCA CATGGACAGT CACCAGCAAC CGGAGTCGTG CAACCACAGC 
AACAGCCCTT CCCTGGTCAC GGTGGGCTAC ACCTCCGTGT CCAGCACGAC CCCGGACTCC 
AACCTCTCGG TGGACAGCTC GACCATGGTG GAAGCCGCCC CACCCATCCC AAAGAGTCGG 
GGGAGGAAGC GGGCCGCTCA GCAGACCCCG GACATGACTG TCCCCTCGAG TAAACAGGCG 
AAGGTCACCT ACAGCTGTAT TTACTGCAAC AAGCAGTTGT TTTCCAGTCT GGCGGTTCTG 
CAGATTCACC TGAAAACTAT GCATTTAGAT AAGCCAGAGC AGGCCCACAT CTGTCAGTAC 
TGCTTGGAGG TACTGCCCTC CCTCTATAAC CTCAATGAAC ATCTTAAGCA AGTGCATGAA 
GCTCAGGACC CAGGTCTGAT CGTTTCTGCC TTGCCCGCCA TAGTCTACCA GTGCAACTTC 
TGCTCTGAAG TTGTCAACGA CCTCAACACC CTTCAGGAAC ACATCCGATG TTCTCACGGA 
TTTGCGAACC CCGCGGCAAA GGACAGCAAT GCTTTCTTCT GTCCCCACTG CTACATGGGG 
TTCCTCACTG ACTCTTCGCT GGAAGAGCAT ATAAGACAGG TCCATTGTGA CCTCAGTGGC 
TCCCGATTTG GGTCCCCAGT GCTCGGGACT CCGAAAGAGC CAGTCGTAGA AGTCTATTCT 
TGTTCCTATT GTACAAATTC CCCCATATTC AACAGCGTTC TTAAACTGAA CAAGCACATC 
AAAGAGAATC ATAAAAACAT TCCCTTGGCC CTGAATTATA TCCACAACGG GAAGAAATCC 
AGGGCCTTGA GTCCCCTGTC ACCTGTGGCC ATAGAGCAGA CATCTCTGAA GATGATGCAG 
GCGGTAGGAG GGGCGCCTGC AAGGCAGGCT GGAGAATATA TCTGTAATCA GTGTGGTGCT 
AAGTACACGT CCCTGGACGG CTTTCAGACT CACCTGAAGA CCCACCTGGA CACCGTGCTC 
CCGAAACTGA CCTGTCCTCA GTGCAACAAG GAGTTTCCCA ACCAAGAGTC CCTGCTGAAG 
CATGTGACCA TTCATTTCAT GATCACCTCC ACGTACTACA TCTGCGAGAG CTGTGACAAG 
CAGTTCACAT CGGTGGACGA CCTGCAGAAA CACCTGCTGG ACATGCACAC CTTCGTCTTC 
TTCCGCTGCA CCCTCTGCCA GGAGGTGTTC GACTCCAAGG TCTCCATCCA ACTCCACCTG 
GCCGTGAAGC ACAGTAATGA GAAGAAAGTA TATCGCTGCA CCTCCTGCAA CTGGGACTTC 
CGCAACGAGA CGGACCTGCA GCTGCACGTG AAGCACAACC ACCTGGAGAA CCAGGGCAAA 
GTGCACAAGT GCATCTTCTG CGGCGAGTCC TTCGGCACTG AGGTGGAGCT GCAGTGTCAC 
ATCACCACGC ACAGCAAGAA GTACAACTGC CAGTTCTGCA GCAAGGCCTT CCATGCCATC 
ATCCTGCTGG AGAAGCACCT GCGGGAGAAG CACTGTGTGT TCGACACCAA GACGCCCAAC 
TGCGGCGCCA ACGGGGCCTC GGAGCCGGGC CAGAAGGAGG AGGTGGAGTT GCAGACCCTG 
CTGACCAACA GCCAGGAGTC CCACAACAGC CACGACGGCA GCGAGGAGGA CGTGGACACC 
TCGGAGCCCA TGTACGGCTG CGACATCTGC GGGGCGGCCT ACACCATGGA GACGCTCCTG 
CAGAACCACC AACTCCGGGA CCACAACATC CGGCCGGGGG AGAGCGCCAT TGTCAAGAAG 
AAAGCCGAGC TCATCAAAGG GAACTACAAG TGCAACGTGT GCTCGCGGAC CTTCTTCTCG 
GAGAACGGCC TCCGGGAGCA CATGCAGACG CACCTGGGCC CCGTGAAACA TTACATGTGC 
CCCATCTGTG GGGAGCGCTT CCCCTCGCTC TTGACCTTGA CCGAACACAA GGTCACGCAC 
AGTAAGAGCC TGGATACAGG AAACTGCCGC ATCTGCAAGA TGCCATTGCA GAGCGAGGAG 
GAGTTCCTGG AGCATTGCCA GATGCACCCC GACCTGCGGA ACTCCCTGAC GGGGTTCCGC 
TGCGTGGTTT GCATGCAGAC GGTGACCTCC ACCCTGGAGC TCAAAATCCA CGGGACGTTC 
CACATGCAGA AGACGGGCAA CGGGGCCACG GTCCAGGCCA CTGGCCGCGG CCCACACGTC 
CCCAAACTGT ACAAGTGTGC GTCCTGTCTC AAAGAATTCC GTTCCAAGCA AGATCTGGTG 
AAACTGGACA TCAACGGCCT GCCCTACGGT CTGTGCGCCG GCTGCGTGAA CCTCAGTAAG 
AGCGCCAGTC CCGGGGTCAA CATCGCTCCC GGCTCCAGTC GACCGGGCCT GGGCCAGACC 
GAGAACCTGG GGGCCGTGGA GGGCAAGGGC AAGGCCGGCG CCCCCAAGAC GCGCTGCTCC 
AGCTGCAACG TGAAGTTTGA GTCCGAAAGT GAACTCCAGA ACCACATTCA AACGGTCCAC 
CGAGAGCTGG TGCCCGACAG CAACAGCACG CAGTTGAAAA CGCCTCAAGT CTCGCCCATG 
CCCAGGATCA GCCCCTCGCA ATCGGATGAG AAGAAGACCT ATCAGTGCAT CAAGTGTCAG 
ATGGTTTTCT ACAATGAATG GGATATTCAG GTGCATGTTG CAAATCACAT GATTGATGAA 
GGGCTGAACC ACGAGTGCAA ACTTTGCAGC CAAACCTTCG ACTCCCCTGC CAAGCTCCAG 
TGCCACCTGA TCGAGCACAG CTTCGAAGGG ATGGGCGGTA CCTTCAAGTG TCCCGTCTGC 
TTTACAGTGT TTGTTCAAGC AAACAAGTTG CAGCAGCATA TTTTCTCTGC CCATGGACAA 
GAAGACAAGA TCTACGACTG CACACAATGT CCACAGAAGT TTTTCTTCCA AACAGAGCTG 
CAGAACCACA CGATGACCCA ACACAGCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_006045477.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006045539.1 Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu04495
Clone ID Related Accession (Same CDS sequence)	XM_006045477.1
Accession Version	XM_006045477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	zinc finger protein 521-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783729.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACATTT GGCAAGGTTG TCATTCTGTA AGAAAAGACC CCAACTGTAA ACTTGAAGAC 
AAGACTGAAG ATGGAGAAGC AATAGACTGT AAGAAGAGGC CGGAAGACGG GGAGGAGCTA 
GAGGACGAAG CTGTGCACAG CTGTGAGAGC TGCCTCCAGG TGTTCGAGTC ACTGAGCGAT 
ATCACAGAAC ACAAGATCAA TCAATGTCAG CTGACAGATG GAGTGGATGT AGAGGATGAC 
CCCACTTGCT CCTGGCCAGC TTCCTCACCT TCGAGCAAGG ACCAGACTTC CCCCAGCCAC 
GGAGAAGGTT GCGATTTTGG AGAGGAAGAA GGTGGCCCCG GACTCCCGTA CCCGTGCCAG 
TTCTGTGACA AGTCATTCAG CCGCCTCAGC TACCTAAAGC ACCATGAGCA GAGTCACAGC 
GACAAGCTGC CTTTCAAGTG CACCTACTGC AGCCGGCTGT TCAAACACAA GCGGAGCCGA 
GACCGCCACA TCAAACTCCA CACCGGGGAC AAGAAGTACC ACTGCAGTGA GTGCGACGCC 
GCCTTCTCCA GAAGTGATCA CCTGAAGATC CACTTGAAGA CTCACACGTC CAACAAGCCA 
TATAAATGTG CCATTTGTCG CCGTGGGTTC CTGTCCTCGA GTTCCTTACA CGGGCACATG 
CAGGTCCACG AGAGGAGCAA GGACGGGTCC CAGTCGGGTT CCAGGATGGA GGACTGGAAG 
ATGAAGGACA CTCAGAAGTG CAGTCAGTGT GAGGAAGGCT TTGACTTCCC GGAAGACCTC 
CAAAAGCACA TCGCCGAGTG CCACCCCGAG TGCTCCCCGA ACGAGGACCG GGCGGCCCTC 
CAGTGCATCT ACTGCCACGA GCTCTTCGTG GAGGAGACCT CCCTGATGAA CCACATGGAG 
CAGATGCACG GCGGGGAGAA GAAGAACTCC TGCAGCATCT GTTCCGAGAG CTTCCACAGC 
GTCGAGGAGC TGTACAGCCA CATGGACAGT CACCAGCAAC CGGAGTCGTG CAACCACAGC 
AACAGCCCTT CCCTGGTCAC GGTGGGCTAC ACCTCCGTGT CCAGCACGAC CCCGGACTCC 
AACCTCTCGG TGGACAGCTC GACCATGGTG GAAGCCGCCC CACCCATCCC AAAGAGTCGG 
GGGAGGAAGC GGGCCGCTCA GCAGACCCCG GACATGACTG TCCCCTCGAG TAAACAGGCG 
AAGGTCACCT ACAGCTGTAT TTACTGCAAC AAGCAGTTGT TTTCCAGTCT GGCGGTTCTG 
CAGATTCACC TGAAAACTAT GCATTTAGAT AAGCCAGAGC AGGCCCACAT CTGTCAGTAC 
TGCTTGGAGG TACTGCCCTC CCTCTATAAC CTCAATGAAC ATCTTAAGCA AGTGCATGAA 
GCTCAGGACC CAGGTCTGAT CGTTTCTGCC TTGCCCGCCA TAGTCTACCA GTGCAACTTC 
TGCTCTGAAG TTGTCAACGA CCTCAACACC CTTCAGGAAC ACATCCGATG TTCTCACGGA 
TTTGCGAACC CCGCGGCAAA GGACAGCAAT GCTTTCTTCT GTCCCCACTG CTACATGGGG 
TTCCTCACTG ACTCTTCGCT GGAAGAGCAT ATAAGACAGG TCCATTGTGA CCTCAGTGGC 
TCCCGATTTG GGTCCCCAGT GCTCGGGACT CCGAAAGAGC CAGTCGTAGA AGTCTATTCT 
TGTTCCTATT GTACAAATTC CCCCATATTC AACAGCGTTC TTAAACTGAA CAAGCACATC 
AAAGAGAATC ATAAAAACAT TCCCTTGGCC CTGAATTATA TCCACAACGG GAAGAAATCC 
AGGGCCTTGA GTCCCCTGTC ACCTGTGGCC ATAGAGCAGA CATCTCTGAA GATGATGCAG 
GCGGTAGGAG GGGCGCCTGC AAGGCAGGCT GGAGAATATA TCTGTAATCA GTGTGGTGCT 
AAGTACACGT CCCTGGACGG CTTTCAGACT CACCTGAAGA CCCACCTGGA CACCGTGCTC 
CCGAAACTGA CCTGTCCTCA GTGCAACAAG GAGTTTCCCA ACCAAGAGTC CCTGCTGAAG 
CATGTGACCA TTCATTTCAT GATCACCTCC ACGTACTACA TCTGCGAGAG CTGTGACAAG 
CAGTTCACAT CGGTGGACGA CCTGCAGAAA CACCTGCTGG ACATGCACAC CTTCGTCTTC 
TTCCGCTGCA CCCTCTGCCA GGAGGTGTTC GACTCCAAGG TCTCCATCCA ACTCCACCTG 
GCCGTGAAGC ACAGTAATGA GAAGAAAGTA TATCGCTGCA CCTCCTGCAA CTGGGACTTC 
CGCAACGAGA CGGACCTGCA GCTGCACGTG AAGCACAACC ACCTGGAGAA CCAGGGCAAA 
GTGCACAAGT GCATCTTCTG CGGCGAGTCC TTCGGCACTG AGGTGGAGCT GCAGTGTCAC 
ATCACCACGC ACAGCAAGAA GTACAACTGC CAGTTCTGCA GCAAGGCCTT CCATGCCATC 
ATCCTGCTGG AGAAGCACCT GCGGGAGAAG CACTGTGTGT TCGACACCAA GACGCCCAAC 
TGCGGCGCCA ACGGGGCCTC GGAGCCGGGC CAGAAGGAGG AGGTGGAGTT GCAGACCCTG 
CTGACCAACA GCCAGGAGTC CCACAACAGC CACGACGGCA GCGAGGAGGA CGTGGACACC 
TCGGAGCCCA TGTACGGCTG CGACATCTGC GGGGCGGCCT ACACCATGGA GACGCTCCTG 
CAGAACCACC AACTCCGGGA CCACAACATC CGGCCGGGGG AGAGCGCCAT TGTCAAGAAG 
AAAGCCGAGC TCATCAAAGG GAACTACAAG TGCAACGTGT GCTCGCGGAC CTTCTTCTCG 
GAGAACGGCC TCCGGGAGCA CATGCAGACG CACCTGGGCC CCGTGAAACA TTACATGTGC 
CCCATCTGTG GGGAGCGCTT CCCCTCGCTC TTGACCTTGA CCGAACACAA GGTCACGCAC 
AGTAAGAGCC TGGATACAGG AAACTGCCGC ATCTGCAAGA TGCCATTGCA GAGCGAGGAG 
GAGTTCCTGG AGCATTGCCA GATGCACCCC GACCTGCGGA ACTCCCTGAC GGGGTTCCGC 
TGCGTGGTTT GCATGCAGAC GGTGACCTCC ACCCTGGAGC TCAAAATCCA CGGGACGTTC 
CACATGCAGA AGACGGGCAA CGGGGCCACG GTCCAGGCCA CTGGCCGCGG CCCACACGTC 
CCCAAACTGT ACAAGTGTGC GTCCTGTCTC AAAGAATTCC GTTCCAAGCA AGATCTGGTG 
AAACTGGACA TCAACGGCCT GCCCTACGGT CTGTGCGCCG GCTGCGTGAA CCTCAGTAAG 
AGCGCCAGTC CCGGGGTCAA CATCGCTCCC GGCTCCAGTC GACCGGGCCT GGGCCAGACC 
GAGAACCTGG GGGCCGTGGA GGGCAAGGGC AAGGCCGGCG CCCCCAAGAC GCGCTGCTCC 
AGCTGCAACG TGAAGTTTGA GTCCGAAAGT GAACTCCAGA ACCACATTCA AACGGTCCAC 
CGAGAGCTGG TGCCCGACAG CAACAGCACG CAGTTGAAAA CGCCTCAAGT CTCGCCCATG 
CCCAGGATCA GCCCCTCGCA ATCGGATGAG AAGACCTATC AGTGCATCAA GTGTCAGATG 
GTTTTCTACA ATGAATGGGA TATTCAGGTG CATGTTGCAA ATCACATGAT TGATGAAGGG 
CTGAACCACG AGTGCAAACT TTGCAGCCAA ACCTTCGACT CCCCTGCCAA GCTCCAGTGC 
CACCTGATCG AGCACAGCTT CGAAGGGATG GGCGGTACCT TCAAGTGTCC CGTCTGCTTT 
ACAGTGTTTG TTCAAGCAAA CAAGTTGCAG CAGCATATTT TCTCTGCCCA TGGACAAGAA 
GACAAGATCT ACGACTGCAC ACAATGTCCA CAGAAGTTTT TCTTCCAAAC AGAGCTGCAG 
AACCACACGA TGACCCAACA CAGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006045539.1
CDS	12..3941
Translation	MHIWQGCHSVRKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCESCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERSKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCIYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKAGAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_006045477.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006045477.1

ORF Insert Sequence:

ATGCACATTT GGCAAGGTTG TCATTCTGTA AGAAAAGACC CCAACTGTAA ACTTGAAGAC 
AAGACTGAAG ATGGAGAAGC AATAGACTGT AAGAAGAGGC CGGAAGACGG GGAGGAGCTA 
GAGGACGAAG CTGTGCACAG CTGTGAGAGC TGCCTCCAGG TGTTCGAGTC ACTGAGCGAT 
ATCACAGAAC ACAAGATCAA TCAATGTCAG CTGACAGATG GAGTGGATGT AGAGGATGAC 
CCCACTTGCT CCTGGCCAGC TTCCTCACCT TCGAGCAAGG ACCAGACTTC CCCCAGCCAC 
GGAGAAGGTT GCGATTTTGG AGAGGAAGAA GGTGGCCCCG GACTCCCGTA CCCGTGCCAG 
TTCTGTGACA AGTCATTCAG CCGCCTCAGC TACCTAAAGC ACCATGAGCA GAGTCACAGC 
GACAAGCTGC CTTTCAAGTG CACCTACTGC AGCCGGCTGT TCAAACACAA GCGGAGCCGA 
GACCGCCACA TCAAACTCCA CACCGGGGAC AAGAAGTACC ACTGCAGTGA GTGCGACGCC 
GCCTTCTCCA GAAGTGATCA CCTGAAGATC CACTTGAAGA CTCACACGTC CAACAAGCCA 
TATAAATGTG CCATTTGTCG CCGTGGGTTC CTGTCCTCGA GTTCCTTACA CGGGCACATG 
CAGGTCCACG AGAGGAGCAA GGACGGGTCC CAGTCGGGTT CCAGGATGGA GGACTGGAAG 
ATGAAGGACA CTCAGAAGTG CAGTCAGTGT GAGGAAGGCT TTGACTTCCC GGAAGACCTC 
CAAAAGCACA TCGCCGAGTG CCACCCCGAG TGCTCCCCGA ACGAGGACCG GGCGGCCCTC 
CAGTGCATCT ACTGCCACGA GCTCTTCGTG GAGGAGACCT CCCTGATGAA CCACATGGAG 
CAGATGCACG GCGGGGAGAA GAAGAACTCC TGCAGCATCT GTTCCGAGAG CTTCCACAGC 
GTCGAGGAGC TGTACAGCCA CATGGACAGT CACCAGCAAC CGGAGTCGTG CAACCACAGC 
AACAGCCCTT CCCTGGTCAC GGTGGGCTAC ACCTCCGTGT CCAGCACGAC CCCGGACTCC 
AACCTCTCGG TGGACAGCTC GACCATGGTG GAAGCCGCCC CACCCATCCC AAAGAGTCGG 
GGGAGGAAGC GGGCCGCTCA GCAGACCCCG GACATGACTG TCCCCTCGAG TAAACAGGCG 
AAGGTCACCT ACAGCTGTAT TTACTGCAAC AAGCAGTTGT TTTCCAGTCT GGCGGTTCTG 
CAGATTCACC TGAAAACTAT GCATTTAGAT AAGCCAGAGC AGGCCCACAT CTGTCAGTAC 
TGCTTGGAGG TACTGCCCTC CCTCTATAAC CTCAATGAAC ATCTTAAGCA AGTGCATGAA 
GCTCAGGACC CAGGTCTGAT CGTTTCTGCC TTGCCCGCCA TAGTCTACCA GTGCAACTTC 
TGCTCTGAAG TTGTCAACGA CCTCAACACC CTTCAGGAAC ACATCCGATG TTCTCACGGA 
TTTGCGAACC CCGCGGCAAA GGACAGCAAT GCTTTCTTCT GTCCCCACTG CTACATGGGG 
TTCCTCACTG ACTCTTCGCT GGAAGAGCAT ATAAGACAGG TCCATTGTGA CCTCAGTGGC 
TCCCGATTTG GGTCCCCAGT GCTCGGGACT CCGAAAGAGC CAGTCGTAGA AGTCTATTCT 
TGTTCCTATT GTACAAATTC CCCCATATTC AACAGCGTTC TTAAACTGAA CAAGCACATC 
AAAGAGAATC ATAAAAACAT TCCCTTGGCC CTGAATTATA TCCACAACGG GAAGAAATCC 
AGGGCCTTGA GTCCCCTGTC ACCTGTGGCC ATAGAGCAGA CATCTCTGAA GATGATGCAG 
GCGGTAGGAG GGGCGCCTGC AAGGCAGGCT GGAGAATATA TCTGTAATCA GTGTGGTGCT 
AAGTACACGT CCCTGGACGG CTTTCAGACT CACCTGAAGA CCCACCTGGA CACCGTGCTC 
CCGAAACTGA CCTGTCCTCA GTGCAACAAG GAGTTTCCCA ACCAAGAGTC CCTGCTGAAG 
CATGTGACCA TTCATTTCAT GATCACCTCC ACGTACTACA TCTGCGAGAG CTGTGACAAG 
CAGTTCACAT CGGTGGACGA CCTGCAGAAA CACCTGCTGG ACATGCACAC CTTCGTCTTC 
TTCCGCTGCA CCCTCTGCCA GGAGGTGTTC GACTCCAAGG TCTCCATCCA ACTCCACCTG 
GCCGTGAAGC ACAGTAATGA GAAGAAAGTA TATCGCTGCA CCTCCTGCAA CTGGGACTTC 
CGCAACGAGA CGGACCTGCA GCTGCACGTG AAGCACAACC ACCTGGAGAA CCAGGGCAAA 
GTGCACAAGT GCATCTTCTG CGGCGAGTCC TTCGGCACTG AGGTGGAGCT GCAGTGTCAC 
ATCACCACGC ACAGCAAGAA GTACAACTGC CAGTTCTGCA GCAAGGCCTT CCATGCCATC 
ATCCTGCTGG AGAAGCACCT GCGGGAGAAG CACTGTGTGT TCGACACCAA GACGCCCAAC 
TGCGGCGCCA ACGGGGCCTC GGAGCCGGGC CAGAAGGAGG AGGTGGAGTT GCAGACCCTG 
CTGACCAACA GCCAGGAGTC CCACAACAGC CACGACGGCA GCGAGGAGGA CGTGGACACC 
TCGGAGCCCA TGTACGGCTG CGACATCTGC GGGGCGGCCT ACACCATGGA GACGCTCCTG 
CAGAACCACC AACTCCGGGA CCACAACATC CGGCCGGGGG AGAGCGCCAT TGTCAAGAAG 
AAAGCCGAGC TCATCAAAGG GAACTACAAG TGCAACGTGT GCTCGCGGAC CTTCTTCTCG 
GAGAACGGCC TCCGGGAGCA CATGCAGACG CACCTGGGCC CCGTGAAACA TTACATGTGC 
CCCATCTGTG GGGAGCGCTT CCCCTCGCTC TTGACCTTGA CCGAACACAA GGTCACGCAC 
AGTAAGAGCC TGGATACAGG AAACTGCCGC ATCTGCAAGA TGCCATTGCA GAGCGAGGAG 
GAGTTCCTGG AGCATTGCCA GATGCACCCC GACCTGCGGA ACTCCCTGAC GGGGTTCCGC 
TGCGTGGTTT GCATGCAGAC GGTGACCTCC ACCCTGGAGC TCAAAATCCA CGGGACGTTC 
CACATGCAGA AGACGGGCAA CGGGGCCACG GTCCAGGCCA CTGGCCGCGG CCCACACGTC 
CCCAAACTGT ACAAGTGTGC GTCCTGTCTC AAAGAATTCC GTTCCAAGCA AGATCTGGTG 
AAACTGGACA TCAACGGCCT GCCCTACGGT CTGTGCGCCG GCTGCGTGAA CCTCAGTAAG 
AGCGCCAGTC CCGGGGTCAA CATCGCTCCC GGCTCCAGTC GACCGGGCCT GGGCCAGACC 
GAGAACCTGG GGGCCGTGGA GGGCAAGGGC AAGGCCGGCG CCCCCAAGAC GCGCTGCTCC 
AGCTGCAACG TGAAGTTTGA GTCCGAAAGT GAACTCCAGA ACCACATTCA AACGGTCCAC 
CGAGAGCTGG TGCCCGACAG CAACAGCACG CAGTTGAAAA CGCCTCAAGT CTCGCCCATG 
CCCAGGATCA GCCCCTCGCA ATCGGATGAG AAGACCTATC AGTGCATCAA GTGTCAGATG 
GTTTTCTACA ATGAATGGGA TATTCAGGTG CATGTTGCAA ATCACATGAT TGATGAAGGG 
CTGAACCACG AGTGCAAACT TTGCAGCCAA ACCTTCGACT CCCCTGCCAA GCTCCAGTGC 
CACCTGATCG AGCACAGCTT CGAAGGGATG GGCGGTACCT TCAAGTGTCC CGTCTGCTTT 
ACAGTGTTTG TTCAAGCAAA CAAGTTGCAG CAGCATATTT TCTCTGCCCA TGGACAAGAA 
GACAAGATCT ACGACTGCAC ACAATGTCCA CAGAAGTTTT TCTTCCAAAC AGAGCTGCAG 
AACCACACGA TGACCCAACA CAGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_025273509.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025129294.1 Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.

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CloneID	OBu58660
Clone ID Related Accession (Same CDS sequence)	XM_025273509.1 , XM_025273508.1
Accession Version	XM_025273509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	zinc finger protein 521 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAG AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGCCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCCT CGAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAG CAAGGACGGG TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGCA TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CCTCCCTGAT GAACCACATG 
GAGCAGATGC ATGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC AGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCCTGGT CACGGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA CGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTTC CCAACCAAGA GTCCCTGCTG 
AAGCATGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CATCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGACTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACGGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CTGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGTG TGTTCGACAC CAAGACGCCC 
AACTGCGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCGGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATTGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCTGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACCT TGACCGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCACAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGCG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCG GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCCGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCTCA AGTCTCGCCC 
ATGCCCAGGA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCATG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TCGACTCCCC TGCCAAGCTC 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ACACGATGAC CCAACACAGC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025129294.1
CDS	260..4195
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCESCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERSKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCIYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKAGAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_025273509.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025273509.1

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAG AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGCCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCCT CGAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAG CAAGGACGGG TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGCA TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CCTCCCTGAT GAACCACATG 
GAGCAGATGC ATGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC AGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCCTGGT CACGGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA CGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTTC CCAACCAAGA GTCCCTGCTG 
AAGCATGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CATCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGACTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACGGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CTGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGTG TGTTCGACAC CAAGACGCCC 
AACTGCGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCGGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATTGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCTGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACCT TGACCGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCACAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGCG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCG GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCCGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCTCA AGTCTCGCCC 
ATGCCCAGGA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCATG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TCGACTCCCC TGCCAAGCTC 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ACACGATGAC CCAACACAGC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_025273508.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025129293.1 Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu58660
Clone ID Related Accession (Same CDS sequence)	XM_025273509.1 , XM_025273508.1
Accession Version	XM_025273508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	zinc finger protein 521 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAG AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGCCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCCT CGAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAG CAAGGACGGG TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGCA TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CCTCCCTGAT GAACCACATG 
GAGCAGATGC ATGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC AGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCCTGGT CACGGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA CGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTTC CCAACCAAGA GTCCCTGCTG 
AAGCATGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CATCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGACTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACGGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CTGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGTG TGTTCGACAC CAAGACGCCC 
AACTGCGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCGGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATTGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCTGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACCT TGACCGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCACAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGCG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCG GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCCGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCTCA AGTCTCGCCC 
ATGCCCAGGA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCATG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TCGACTCCCC TGCCAAGCTC 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ACACGATGAC CCAACACAGC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025129293.1
CDS	132..4067
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCESCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERSKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCIYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKAGAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_025273508.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025273508.1

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAG AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGCCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCCT CGAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAG CAAGGACGGG TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGCA TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CCTCCCTGAT GAACCACATG 
GAGCAGATGC ATGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC AGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCCTGGT CACGGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA CGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTTC CCAACCAAGA GTCCCTGCTG 
AAGCATGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CATCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGACTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACGGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CTGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGTG TGTTCGACAC CAAGACGCCC 
AACTGCGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCGGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATTGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCTGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACCT TGACCGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCACAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGCG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCG GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCCGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCTCA AGTCTCGCCC 
ATGCCCAGGA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCATG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TCGACTCCCC TGCCAAGCTC 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ACACGATGAC CCAACACAGC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_006045476.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006045538.1 Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu58661
Clone ID Related Accession (Same CDS sequence)	XM_006045476.2
Accession Version	XM_006045476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	zinc finger protein 521 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006045476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACATTT GGCAAGGTTG TCATTCTGTA AGAAAAGACC CCAACTGTAA ACTTGAAGAC 
AAGACTGAAG ATGGAGAAGC AATAGACTGT AAGAAGAGGC CGGAAGACGG GGAGGAGCTA 
GAGGACGAAG CTGTGCACAG CTGTGAGAGC TGCCTCCAGG TGTTCGAGTC ACTGAGCGAT 
ATCACAGAAC ACAAGATCAA TCAATGTCAG CTGACAGATG GAGTGGATGT AGAGGATGAC 
CCCACTTGCT CCTGGCCAGC TTCCTCACCT TCGAGCAAGG ACCAGACTTC CCCCAGCCAC 
GGAGAAGGTT GCGATTTTGG AGAGGAAGAA GGTGGCCCCG GACTCCCGTA CCCGTGCCAG 
TTCTGTGACA AGTCATTCAG CCGCCTCAGC TACCTAAAGC ACCATGAGCA GAGTCACAGC 
GACAAGCTGC CTTTCAAGTG CACCTACTGC AGCCGGCTGT TCAAACACAA GCGGAGCCGA 
GACCGCCACA TCAAACTCCA CACCGGGGAC AAGAAGTACC ACTGCAGTGA GTGCGACGCC 
GCCTTCTCCA GAAGTGATCA CCTGAAGATC CACTTGAAGA CTCACACGTC CAACAAGCCA 
TATAAATGTG CCATTTGTCG CCGTGGGTTC CTGTCCTCGA GTTCCTTACA CGGGCACATG 
CAGGTCCACG AGAGGAGCAA GGACGGGTCC CAGTCGGGTT CCAGGATGGA GGACTGGAAG 
ATGAAGGACA CTCAGAAGTG CAGTCAGTGT GAGGAAGGCT TTGACTTCCC GGAAGACCTC 
CAGAAGCACA TCGCCGAGTG CCACCCCGAG TGCTCCCCGA ACGAGGACCG GGCGGCCCTC 
CAGTGCATCT ACTGCCACGA GCTCTTCGTG GAGGAGACCT CCCTGATGAA CCACATGGAG 
CAGATGCATG GCGGGGAGAA GAAGAACTCC TGCAGCATCT GTTCCGAGAG CTTCCACAGC 
GTCGAGGAGC TGTACAGCCA CATGGACAGT CACCAGCAAC CGGAGTCGTG CAACCACAGC 
AACAGCCCTT CCCTGGTCAC GGTGGGCTAC ACCTCCGTGT CCAGCACGAC CCCGGACTCC 
AACCTCTCGG TGGACAGCTC GACCATGGTG GAAGCCGCCC CACCCATCCC AAAGAGTCGG 
GGGAGGAAGC GGGCCGCTCA GCAGACCCCG GACATGACTG TCCCCTCGAG TAAACAGGCG 
AAGGTCACCT ACAGCTGTAT TTACTGCAAC AAGCAGTTGT TTTCCAGTCT GGCGGTTCTG 
CAGATTCACC TGAAAACTAT GCATTTAGAT AAGCCAGAGC AGGCCCACAT CTGTCAGTAC 
TGCTTGGAGG TACTGCCCTC CCTCTATAAC CTCAATGAAC ATCTTAAGCA AGTGCATGAA 
GCTCAGGACC CAGGTCTGAT CGTTTCTGCC TTGCCCGCCA TAGTCTACCA GTGCAACTTC 
TGCTCTGAAG TTGTCAACGA CCTCAACACC CTTCAGGAAC ACATCCGATG TTCTCACGGA 
TTTGCGAACC CCGCGGCAAA GGACAGCAAT GCTTTCTTCT GTCCCCACTG CTACATGGGG 
TTCCTCACTG ACTCTTCGCT GGAAGAGCAT ATAAGACAGG TCCATTGTGA CCTCAGTGGC 
TCCCGATTTG GGTCCCCAGT GCTCGGGACT CCGAAAGAGC CAGTCGTAGA AGTCTATTCT 
TGTTCCTATT GTACAAATTC CCCCATATTC AACAGCGTTC TTAAACTGAA CAAGCACATC 
AAAGAGAATC ATAAAAACAT TCCCTTGGCC CTGAATTATA TCCACAACGG GAAGAAATCC 
AGGGCCTTGA GTCCCCTGTC ACCTGTGGCC ATAGAGCAGA CATCTCTGAA GATGATGCAG 
GCGGTAGGAG GGGCGCCTGC AAGGCAGGCT GGAGAATATA TCTGTAATCA GTGTGGTGCT 
AAGTACACGT CCCTGGACGG CTTTCAGACT CACCTGAAGA CCCACCTGGA CACCGTGCTC 
CCGAAACTGA CCTGTCCTCA GTGCAACAAG GAGTTTCCCA ACCAAGAGTC CCTGCTGAAG 
CATGTGACCA TTCATTTCAT GATCACCTCC ACGTACTACA TCTGCGAGAG CTGTGACAAG 
CAGTTCACAT CGGTGGACGA CCTGCAGAAA CACCTGCTGG ACATGCACAC CTTCGTCTTC 
TTCCGCTGCA CCCTCTGCCA GGAGGTGTTC GACTCCAAGG TCTCCATCCA ACTCCACCTG 
GCCGTGAAGC ACAGTAATGA GAAGAAAGTA TATCGCTGCA CCTCCTGCAA CTGGGACTTC 
CGCAACGAGA CGGACCTGCA GCTGCACGTG AAGCACAACC ACCTGGAGAA CCAGGGCAAA 
GTGCACAAGT GCATCTTCTG CGGCGAGTCC TTCGGCACTG AGGTGGAGCT GCAGTGTCAC 
ATCACCACGC ACAGCAAGAA GTACAACTGC CAGTTCTGCA GCAAGGCCTT CCATGCCATC 
ATCCTGCTGG AGAAGCACCT GCGGGAGAAG CACTGTGTGT TCGACACCAA GACGCCCAAC 
TGCGGCGCCA ACGGGGCCTC GGAGCCGGGC CAGAAGGAGG AGGTGGAGTT GCAGACCCTG 
CTGACCAACA GCCAGGAGTC CCACAACAGC CACGACGGCA GCGAGGAGGA CGTGGACACC 
TCGGAGCCCA TGTACGGCTG CGACATCTGC GGGGCGGCCT ACACCATGGA GACGCTCCTG 
CAGAACCACC AACTCCGGGA CCACAACATC CGGCCGGGGG AGAGCGCCAT TGTCAAGAAG 
AAAGCCGAGC TCATCAAAGG GAACTACAAG TGCAACGTGT GCTCGCGGAC CTTCTTCTCG 
GAGAACGGCC TCCGGGAGCA CATGCAGACG CACCTGGGCC CTGTGAAACA TTACATGTGC 
CCCATCTGTG GGGAGCGCTT CCCCTCGCTC TTGACCTTGA CCGAACACAA GGTCACGCAC 
AGTAAGAGCC TGGATACAGG AAACTGCCGC ATCTGCAAGA TGCCATTGCA GAGCGAGGAG 
GAGTTCCTGG AGCATTGCCA GATGCACCCC GACCTGCGGA ACTCCCTGAC GGGGTTCCGC 
TGCGTGGTTT GCATGCAGAC GGTGACCTCC ACCCTGGAGC TCAAAATCCA CGGGACGTTC 
CACATGCAGA AGACGGGCAA CGGGGCCACG GTCCAGGCCA CTGGCCGCGG CCCACACGTC 
CCCAAACTGT ACAAGTGTGC GTCCTGTCTC AAAGAATTCC GTTCCAAGCA AGATCTGGTG 
AAACTGGACA TCAACGGCCT GCCCTACGGT CTGTGCGCCG GCTGCGTGAA CCTCAGTAAG 
AGCGCCAGTC CCGGGGTCAA CATCGCTCCC GGCTCCAGTC GACCGGGCCT GGGCCAGACC 
GAGAACCTGG GGGCCGTGGA GGGCAAGGGC AAGGCCGGCG CCCCCAAGAC GCGCTGCTCC 
AGCTGCAACG TGAAGTTTGA GTCCGAAAGT GAACTCCAGA ACCACATTCA AACGGTCCAC 
CGAGAGCTGG TGCCCGACAG CAACAGCACG CAGTTGAAAA CGCCTCAAGT CTCGCCCATG 
CCCAGGATCA GCCCCTCGCA ATCGGATGAG AAGAAGACCT ATCAGTGCAT CAAGTGTCAG 
ATGGTTTTCT ACAATGAATG GGATATTCAG GTGCATGTTG CAAATCACAT GATTGATGAA 
GGGCTGAACC ACGAGTGCAA ACTTTGCAGC CAAACCTTCG ACTCCCCTGC CAAGCTCCAG 
TGCCACCTGA TCGAGCACAG CTTCGAAGGG ATGGGCGGTA CCTTCAAGTG TCCCGTCTGC 
TTTACAGTGT TTGTTCAAGC AAACAAGTTG CAGCAGCATA TTTTCTCTGC CCATGGACAA 
GAAGACAAGA TCTACGACTG CACACAATGT CCACAGAAGT TTTTCTTCCA AACAGAGCTG 
CAGAACCACA CGATGACCCA ACACAGCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006045538.1
CDS	930..4862
Translation	MHIWQGCHSVRKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCESCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERSKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCIYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKAGAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_006045476.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006045476.2

ORF Insert Sequence:

ATGCACATTT GGCAAGGTTG TCATTCTGTA AGAAAAGACC CCAACTGTAA ACTTGAAGAC 
AAGACTGAAG ATGGAGAAGC AATAGACTGT AAGAAGAGGC CGGAAGACGG GGAGGAGCTA 
GAGGACGAAG CTGTGCACAG CTGTGAGAGC TGCCTCCAGG TGTTCGAGTC ACTGAGCGAT 
ATCACAGAAC ACAAGATCAA TCAATGTCAG CTGACAGATG GAGTGGATGT AGAGGATGAC 
CCCACTTGCT CCTGGCCAGC TTCCTCACCT TCGAGCAAGG ACCAGACTTC CCCCAGCCAC 
GGAGAAGGTT GCGATTTTGG AGAGGAAGAA GGTGGCCCCG GACTCCCGTA CCCGTGCCAG 
TTCTGTGACA AGTCATTCAG CCGCCTCAGC TACCTAAAGC ACCATGAGCA GAGTCACAGC 
GACAAGCTGC CTTTCAAGTG CACCTACTGC AGCCGGCTGT TCAAACACAA GCGGAGCCGA 
GACCGCCACA TCAAACTCCA CACCGGGGAC AAGAAGTACC ACTGCAGTGA GTGCGACGCC 
GCCTTCTCCA GAAGTGATCA CCTGAAGATC CACTTGAAGA CTCACACGTC CAACAAGCCA 
TATAAATGTG CCATTTGTCG CCGTGGGTTC CTGTCCTCGA GTTCCTTACA CGGGCACATG 
CAGGTCCACG AGAGGAGCAA GGACGGGTCC CAGTCGGGTT CCAGGATGGA GGACTGGAAG 
ATGAAGGACA CTCAGAAGTG CAGTCAGTGT GAGGAAGGCT TTGACTTCCC GGAAGACCTC 
CAGAAGCACA TCGCCGAGTG CCACCCCGAG TGCTCCCCGA ACGAGGACCG GGCGGCCCTC 
CAGTGCATCT ACTGCCACGA GCTCTTCGTG GAGGAGACCT CCCTGATGAA CCACATGGAG 
CAGATGCATG GCGGGGAGAA GAAGAACTCC TGCAGCATCT GTTCCGAGAG CTTCCACAGC 
GTCGAGGAGC TGTACAGCCA CATGGACAGT CACCAGCAAC CGGAGTCGTG CAACCACAGC 
AACAGCCCTT CCCTGGTCAC GGTGGGCTAC ACCTCCGTGT CCAGCACGAC CCCGGACTCC 
AACCTCTCGG TGGACAGCTC GACCATGGTG GAAGCCGCCC CACCCATCCC AAAGAGTCGG 
GGGAGGAAGC GGGCCGCTCA GCAGACCCCG GACATGACTG TCCCCTCGAG TAAACAGGCG 
AAGGTCACCT ACAGCTGTAT TTACTGCAAC AAGCAGTTGT TTTCCAGTCT GGCGGTTCTG 
CAGATTCACC TGAAAACTAT GCATTTAGAT AAGCCAGAGC AGGCCCACAT CTGTCAGTAC 
TGCTTGGAGG TACTGCCCTC CCTCTATAAC CTCAATGAAC ATCTTAAGCA AGTGCATGAA 
GCTCAGGACC CAGGTCTGAT CGTTTCTGCC TTGCCCGCCA TAGTCTACCA GTGCAACTTC 
TGCTCTGAAG TTGTCAACGA CCTCAACACC CTTCAGGAAC ACATCCGATG TTCTCACGGA 
TTTGCGAACC CCGCGGCAAA GGACAGCAAT GCTTTCTTCT GTCCCCACTG CTACATGGGG 
TTCCTCACTG ACTCTTCGCT GGAAGAGCAT ATAAGACAGG TCCATTGTGA CCTCAGTGGC 
TCCCGATTTG GGTCCCCAGT GCTCGGGACT CCGAAAGAGC CAGTCGTAGA AGTCTATTCT 
TGTTCCTATT GTACAAATTC CCCCATATTC AACAGCGTTC TTAAACTGAA CAAGCACATC 
AAAGAGAATC ATAAAAACAT TCCCTTGGCC CTGAATTATA TCCACAACGG GAAGAAATCC 
AGGGCCTTGA GTCCCCTGTC ACCTGTGGCC ATAGAGCAGA CATCTCTGAA GATGATGCAG 
GCGGTAGGAG GGGCGCCTGC AAGGCAGGCT GGAGAATATA TCTGTAATCA GTGTGGTGCT 
AAGTACACGT CCCTGGACGG CTTTCAGACT CACCTGAAGA CCCACCTGGA CACCGTGCTC 
CCGAAACTGA CCTGTCCTCA GTGCAACAAG GAGTTTCCCA ACCAAGAGTC CCTGCTGAAG 
CATGTGACCA TTCATTTCAT GATCACCTCC ACGTACTACA TCTGCGAGAG CTGTGACAAG 
CAGTTCACAT CGGTGGACGA CCTGCAGAAA CACCTGCTGG ACATGCACAC CTTCGTCTTC 
TTCCGCTGCA CCCTCTGCCA GGAGGTGTTC GACTCCAAGG TCTCCATCCA ACTCCACCTG 
GCCGTGAAGC ACAGTAATGA GAAGAAAGTA TATCGCTGCA CCTCCTGCAA CTGGGACTTC 
CGCAACGAGA CGGACCTGCA GCTGCACGTG AAGCACAACC ACCTGGAGAA CCAGGGCAAA 
GTGCACAAGT GCATCTTCTG CGGCGAGTCC TTCGGCACTG AGGTGGAGCT GCAGTGTCAC 
ATCACCACGC ACAGCAAGAA GTACAACTGC CAGTTCTGCA GCAAGGCCTT CCATGCCATC 
ATCCTGCTGG AGAAGCACCT GCGGGAGAAG CACTGTGTGT TCGACACCAA GACGCCCAAC 
TGCGGCGCCA ACGGGGCCTC GGAGCCGGGC CAGAAGGAGG AGGTGGAGTT GCAGACCCTG 
CTGACCAACA GCCAGGAGTC CCACAACAGC CACGACGGCA GCGAGGAGGA CGTGGACACC 
TCGGAGCCCA TGTACGGCTG CGACATCTGC GGGGCGGCCT ACACCATGGA GACGCTCCTG 
CAGAACCACC AACTCCGGGA CCACAACATC CGGCCGGGGG AGAGCGCCAT TGTCAAGAAG 
AAAGCCGAGC TCATCAAAGG GAACTACAAG TGCAACGTGT GCTCGCGGAC CTTCTTCTCG 
GAGAACGGCC TCCGGGAGCA CATGCAGACG CACCTGGGCC CTGTGAAACA TTACATGTGC 
CCCATCTGTG GGGAGCGCTT CCCCTCGCTC TTGACCTTGA CCGAACACAA GGTCACGCAC 
AGTAAGAGCC TGGATACAGG AAACTGCCGC ATCTGCAAGA TGCCATTGCA GAGCGAGGAG 
GAGTTCCTGG AGCATTGCCA GATGCACCCC GACCTGCGGA ACTCCCTGAC GGGGTTCCGC 
TGCGTGGTTT GCATGCAGAC GGTGACCTCC ACCCTGGAGC TCAAAATCCA CGGGACGTTC 
CACATGCAGA AGACGGGCAA CGGGGCCACG GTCCAGGCCA CTGGCCGCGG CCCACACGTC 
CCCAAACTGT ACAAGTGTGC GTCCTGTCTC AAAGAATTCC GTTCCAAGCA AGATCTGGTG 
AAACTGGACA TCAACGGCCT GCCCTACGGT CTGTGCGCCG GCTGCGTGAA CCTCAGTAAG 
AGCGCCAGTC CCGGGGTCAA CATCGCTCCC GGCTCCAGTC GACCGGGCCT GGGCCAGACC 
GAGAACCTGG GGGCCGTGGA GGGCAAGGGC AAGGCCGGCG CCCCCAAGAC GCGCTGCTCC 
AGCTGCAACG TGAAGTTTGA GTCCGAAAGT GAACTCCAGA ACCACATTCA AACGGTCCAC 
CGAGAGCTGG TGCCCGACAG CAACAGCACG CAGTTGAAAA CGCCTCAAGT CTCGCCCATG 
CCCAGGATCA GCCCCTCGCA ATCGGATGAG AAGAAGACCT ATCAGTGCAT CAAGTGTCAG 
ATGGTTTTCT ACAATGAATG GGATATTCAG GTGCATGTTG CAAATCACAT GATTGATGAA 
GGGCTGAACC ACGAGTGCAA ACTTTGCAGC CAAACCTTCG ACTCCCCTGC CAAGCTCCAG 
TGCCACCTGA TCGAGCACAG CTTCGAAGGG ATGGGCGGTA CCTTCAAGTG TCCCGTCTGC 
TTTACAGTGT TTGTTCAAGC AAACAAGTTG CAGCAGCATA TTTTCTCTGC CCATGGACAA 
GAAGACAAGA TCTACGACTG CACACAATGT CCACAGAAGT TTTTCTTCCA AACAGAGCTG 
CAGAACCACA CGATGACCCA ACACAGCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_025273510.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025129295.1 Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X4, mRNA.

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CloneID	OBu58662
Clone ID Related Accession (Same CDS sequence)	XM_025273510.1
Accession Version	XM_025273510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	zinc finger protein 521 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAG AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGCCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCCT CGAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAG CAAGGACGGG TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGCA TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CCTCCCTGAT GAACCACATG 
GAGCAGATGC ATGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC AGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCCTGGT CACGGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA CGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTTC CCAACCAAGA GTCCCTGCTG 
AAGCATGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CATCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGACTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACGGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CTGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGTG TGTTCGACAC CAAGACGCCC 
AACTGCGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCGGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATTGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCTGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACCT TGACCGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCACAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGCG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCG GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCCGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCTCA AGTCTCGCCC 
ATGCCCAGGA TCAGCCCCTC GCAATCGGAT GAGAAGACCT ATCAGTGCAT CAAGTGTCAG 
ATGGTTTTCT ACAATGAATG GGATATTCAG GTGCATGTTG CAAATCACAT GATTGATGAA 
GGGCTGAACC ACGAGTGCAA ACTTTGCAGC CAAACCTTCG ACTCCCCTGC CAAGCTCCAG 
TGCCACCTGA TCGAGCACAG CTTCGAAGGG ATGGGCGGTA CCTTCAAGTG TCCCGTCTGC 
TTTACAGTGT TTGTTCAAGC AAACAAGTTG CAGCAGCATA TTTTCTCTGC CCATGGACAA 
GAAGACAAGA TCTACGACTG CACACAATGT CCACAGAAGT TTTTCTTCCA AACAGAGCTG 
CAGAACCACA CGATGACCCA ACACAGCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025129295.1
CDS	132..4064
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCESCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERSKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCIYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKAGAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_025273510.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025273510.1

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA AGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGGGAGGAG 
CTAGAGGACG AAGCTGTGCA CAGCTGTGAG AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACAG AACACAAGAT CAATCAATGT CAGCTGACAG ATGGAGTGGA TGTAGAGGAT 
GACCCCACTT GCTCCTGGCC AGCTTCCTCA CCTTCGAGCA AGGACCAGAC TTCCCCCAGC 
CACGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CCGGACTCCC GTACCCGTGC 
CAGTTCTGTG ACAAGTCATT CAGCCGCCTC AGCTACCTAA AGCACCATGA GCAGAGTCAC 
AGCGACAAGC TGCCTTTCAA GTGCACCTAC TGCAGCCGGC TGTTCAAACA CAAGCGGAGC 
CGAGACCGCC ACATCAAACT CCACACCGGG GACAAGAAGT ACCACTGCAG TGAGTGCGAC 
GCCGCCTTCT CCAGAAGTGA TCACCTGAAG ATCCACTTGA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCCT CGAGTTCCTT ACACGGGCAC 
ATGCAGGTCC ACGAGAGGAG CAAGGACGGG TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGCA TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CCTCCCTGAT GAACCACATG 
GAGCAGATGC ATGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC AGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCCTGGT CACGGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA CGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTTC CCAACCAAGA GTCCCTGCTG 
AAGCATGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CATCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGACTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACGGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CTGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGTG TGTTCGACAC CAAGACGCCC 
AACTGCGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCGGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATTGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCTGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACCT TGACCGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCACAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGCG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCG GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCCGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCTCA AGTCTCGCCC 
ATGCCCAGGA TCAGCCCCTC GCAATCGGAT GAGAAGACCT ATCAGTGCAT CAAGTGTCAG 
ATGGTTTTCT ACAATGAATG GGATATTCAG GTGCATGTTG CAAATCACAT GATTGATGAA 
GGGCTGAACC ACGAGTGCAA ACTTTGCAGC CAAACCTTCG ACTCCCCTGC CAAGCTCCAG 
TGCCACCTGA TCGAGCACAG CTTCGAAGGG ATGGGCGGTA CCTTCAAGTG TCCCGTCTGC 
TTTACAGTGT TTGTTCAAGC AAACAAGTTG CAGCAGCATA TTTTCTCTGC CCATGGACAA 
GAAGACAAGA TCTACGACTG CACACAATGT CCACAGAAGT TTTTCTTCCA AACAGAGCTG 
CAGAACCACA CGATGACCCA ACACAGCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_025273511.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025129296.1 Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X5, mRNA.

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CloneID	OBu58663
Clone ID Related Accession (Same CDS sequence)	XM_025273511.1
Accession Version	XM_025273511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	zinc finger protein 521 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037566.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTCC ACGAGAGGAG CAAGGACGGG TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGCA TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CCTCCCTGAT GAACCACATG 
GAGCAGATGC ATGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC AGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCCTGGT CACGGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA CGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTTC CCAACCAAGA GTCCCTGCTG 
AAGCATGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CATCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGACTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACGGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CTGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGTG TGTTCGACAC CAAGACGCCC 
AACTGCGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCGGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATTGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCTGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACCT TGACCGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCACAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGCG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCG GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCCGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCTCA AGTCTCGCCC 
ATGCCCAGGA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCATG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TCGACTCCCC TGCCAAGCTC 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ACACGATGAC CCAACACAGC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025129296.1
CDS	821..4096
Translation	MQVHERSKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCIYCHELFVEETSLMNHMEQMHGGEKKNSCSICSESFHSVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSALPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARQAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCQFCSKAFHAIILLEKHLREKHCVFDTKTPNCGANGASEPGQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGATVQATGRGPHVPKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSASPGVNIAPGSSRPGLGQTENLGAVEGKGKAGAPKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_025273511.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis zinc finger protein 521 (ZNF521), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025273511.1

ORF Insert Sequence:

ATGCAGGTCC ACGAGAGGAG CAAGGACGGG TCCCAGTCGG GTTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGTGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGCTCCC CGAACGAGGA CCGGGCGGCC 
CTCCAGTGCA TCTACTGCCA CGAGCTCTTC GTGGAGGAGA CCTCCCTGAT GAACCACATG 
GAGCAGATGC ATGGCGGGGA GAAGAAGAAC TCCTGCAGCA TCTGTTCCGA GAGCTTCCAC 
AGCGTCGAGG AGCTGTACAG CCACATGGAC AGTCACCAGC AACCGGAGTC GTGCAACCAC 
AGCAACAGCC CTTCCCTGGT CACGGTGGGC TACACCTCCG TGTCCAGCAC GACCCCGGAC 
TCCAACCTCT CGGTGGACAG CTCGACCATG GTGGAAGCCG CCCCACCCAT CCCAAAGAGT 
CGGGGGAGGA AGCGGGCCGC TCAGCAGACC CCGGACATGA CTGTCCCCTC GAGTAAACAG 
GCGAAGGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TGTTTTCCAG TCTGGCGGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AGCAGGCCCA CATCTGTCAG 
TACTGCTTGG AGGTACTGCC CTCCCTCTAT AACCTCAATG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCAGGTCT GATCGTTTCT GCCTTGCCCG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCTG AAGTTGTCAA CGACCTCAAC ACCCTTCAGG AACACATCCG ATGTTCTCAC 
GGATTTGCGA ACCCCGCGGC AAAGGACAGC AATGCTTTCT TCTGTCCCCA CTGCTACATG 
GGGTTCCTCA CTGACTCTTC GCTGGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGAT TTGGGTCCCC AGTGCTCGGG ACTCCGAAAG AGCCAGTCGT AGAAGTCTAT 
TCTTGTTCCT ATTGTACAAA TTCCCCCATA TTCAACAGCG TTCTTAAACT GAACAAGCAC 
ATCAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATCCACAA CGGGAAGAAA 
TCCAGGGCCT TGAGTCCCCT GTCACCTGTG GCCATAGAGC AGACATCTCT GAAGATGATG 
CAGGCGGTAG GAGGGGCGCC TGCAAGGCAG GCTGGAGAAT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTGGA CGGCTTTCAG ACTCACCTGA AGACCCACCT GGACACCGTG 
CTCCCGAAAC TGACCTGTCC TCAGTGCAAC AAGGAGTTTC CCAACCAAGA GTCCCTGCTG 
AAGCATGTGA CCATTCATTT CATGATCACC TCCACGTACT ACATCTGCGA GAGCTGTGAC 
AAGCAGTTCA CATCGGTGGA CGACCTGCAG AAACACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACCCTCTG CCAGGAGGTG TTCGACTCCA AGGTCTCCAT CCAACTCCAC 
CTGGCCGTGA AGCACAGTAA TGAGAAGAAA GTATATCGCT GCACCTCCTG CAACTGGGAC 
TTCCGCAACG AGACGGACCT GCAGCTGCAC GTGAAGCACA ACCACCTGGA GAACCAGGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCCTTCGGCA CTGAGGTGGA GCTGCAGTGT 
CACATCACCA CGCACAGCAA GAAGTACAAC TGCCAGTTCT GCAGCAAGGC CTTCCATGCC 
ATCATCCTGC TGGAGAAGCA CCTGCGGGAG AAGCACTGTG TGTTCGACAC CAAGACGCCC 
AACTGCGGCG CCAACGGGGC CTCGGAGCCG GGCCAGAAGG AGGAGGTGGA GTTGCAGACC 
CTGCTGACCA ACAGCCAGGA GTCCCACAAC AGCCACGACG GCAGCGAGGA GGACGTGGAC 
ACCTCGGAGC CCATGTACGG CTGCGACATC TGCGGGGCGG CCTACACCAT GGAGACGCTC 
CTGCAGAACC ACCAACTCCG GGACCACAAC ATCCGGCCGG GGGAGAGCGC CATTGTCAAG 
AAGAAAGCCG AGCTCATCAA AGGGAACTAC AAGTGCAACG TGTGCTCGCG GACCTTCTTC 
TCGGAGAACG GCCTCCGGGA GCACATGCAG ACGCACCTGG GCCCTGTGAA ACATTACATG 
TGCCCCATCT GTGGGGAGCG CTTCCCCTCG CTCTTGACCT TGACCGAACA CAAGGTCACG 
CACAGTAAGA GCCTGGATAC AGGAAACTGC CGCATCTGCA AGATGCCATT GCAGAGCGAG 
GAGGAGTTCC TGGAGCATTG CCAGATGCAC CCCGACCTGC GGAACTCCCT GACGGGGTTC 
CGCTGCGTGG TTTGCATGCA GACGGTGACC TCCACCCTGG AGCTCAAAAT CCACGGGACG 
TTCCACATGC AGAAGACGGG CAACGGGGCC ACGGTCCAGG CCACTGGCCG CGGCCCACAC 
GTCCCCAAAC TGTACAAGTG TGCGTCCTGT CTCAAAGAAT TCCGTTCCAA GCAAGATCTG 
GTGAAACTGG ACATCAACGG CCTGCCCTAC GGTCTGTGCG CCGGCTGCGT GAACCTCAGT 
AAGAGCGCCA GTCCCGGGGT CAACATCGCT CCCGGCTCCA GTCGACCGGG CCTGGGCCAG 
ACCGAGAACC TGGGGGCCGT GGAGGGCAAG GGCAAGGCCG GCGCCCCCAA GACGCGCTGC 
TCCAGCTGCA ACGTGAAGTT TGAGTCCGAA AGTGAACTCC AGAACCACAT TCAAACGGTC 
CACCGAGAGC TGGTGCCCGA CAGCAACAGC ACGCAGTTGA AAACGCCTCA AGTCTCGCCC 
ATGCCCAGGA TCAGCCCCTC GCAATCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTGCATG TTGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCACGAGTG CAAACTTTGC AGCCAAACCT TCGACTCCCC TGCCAAGCTC 
CAGTGCCACC TGATCGAGCA CAGCTTCGAA GGGATGGGCG GTACCTTCAA GTGTCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTACGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ACACGATGAC CCAACACAGC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ZNF521 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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