HFM1 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following HFM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HFM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu11903 XM_006054478.1
Latest version!
Bubalus bubalis HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (HFM1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBu68412 XM_025288292.1
Latest version!
Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu68412 XM_025288290.1
Latest version!
Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu68412 XM_025288293.1
Latest version!
Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu68412 XM_025288291.1
Latest version!
Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu68413 XM_025288294.1
Latest version!
Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu68414 XM_025288296.1
Latest version!
Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu68415 XM_025288297.1
Latest version!
Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu68415 XM_025288298.1
Latest version!
Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu68416 XM_025288299.1
Latest version!
Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu68417 XM_025288300.1
Latest version!
Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu11903
    Clone ID Related Accession (Same CDS sequence) XM_006054478.1
    Accession Version XM_006054478.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4317bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product LOW QUALITY PROTEIN: probable ATP-dependent DNA helicase HFM1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784621.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT ATTGTTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCATGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA CATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCTGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CGCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATGTACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCTAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACTG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCTGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCAC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATACAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTAATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAGATCTTA GCATAAATCT AATCAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGGCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GCAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAGAAGTC AGAAGTGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AACAAATCTC CAAATGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTGTAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACAATCAT CTGCATATCA AGACACAGAA
    GTTTTGAAAG TGAACTTTGA ATTGGGAAAT GAAGTTTGGG ATGACTTTGA TGATGAAAAC
    TTAGTAGAAA TTACTAGCTT TTCAGCTGAA ACTGAGAAGA CAAAAACATC AGGATTTGGA
    AACACTTTGA GTTCAAGTAC TGGGGAAAGT AAGCTACCAT TCCAAGAATC AAAAGGCCAA
    TTCCAAAGAG AATTGTCTAA GAGTTTTGTT TCATCACATG AAAAGCCAGA TATTTATTTA
    TCAAATTCTG CTGTGTCCAA GTTTGGTGCA TCCTCCATGA CACAATTACC TCAACAATCT
    GGAAATGCAA GTATTGTCCA TTTACAAGAA AGAAAACAAC AAAGTCTGTC ACCAGAGATT
    GAAAAGCTAT ACTTTACTCG CTCTGAAAAA ATATCAAACT CTTCAAATTT TAATGAGAAA
    GTGGATTGCT TTATTAGAAA TGAATGTAAA AAGGAGATTG ATTTCAGTAG GTATCATCCT
    GATGATGAAG CTGACGAAAT AAAGTCTTTT CTGGGCATAT TTGATGGTAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006054540.1
    CDS9..4325
    Translation

    Target ORF information:

    RefSeq Version XM_006054478.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (HFM1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006054478.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT ATTGTTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCATGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA CATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCTGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CGCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATGTACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCTAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAGCCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACTG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCTGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCAC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATACAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTAATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAGATCTTA GCATAAATCT AATCAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGGCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GCAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAGAAGTC AGAAGTGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AACAAATCTC CAAATGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTGTAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACAATCAT CTGCATATCA AGACACAGAA
    GTTTTGAAAG TGAACTTTGA ATTGGGAAAT GAAGTTTGGG ATGACTTTGA TGATGAAAAC
    TTAGTAGAAA TTACTAGCTT TTCAGCTGAA ACTGAGAAGA CAAAAACATC AGGATTTGGA
    AACACTTTGA GTTCAAGTAC TGGGGAAAGT AAGCTACCAT TCCAAGAATC AAAAGGCCAA
    TTCCAAAGAG AATTGTCTAA GAGTTTTGTT TCATCACATG AAAAGCCAGA TATTTATTTA
    TCAAATTCTG CTGTGTCCAA GTTTGGTGCA TCCTCCATGA CACAATTACC TCAACAATCT
    GGAAATGCAA GTATTGTCCA TTTACAAGAA AGAAAACAAC AAAGTCTGTC ACCAGAGATT
    GAAAAGCTAT ACTTTACTCG CTCTGAAAAA ATATCAAACT CTTCAAATTT TAATGAGAAA
    GTGGATTGCT TTATTAGAAA TGAATGTAAA AAGGAGATTG ATTTCAGTAG GTATCATCCT
    GATGATGAAG CTGACGAAAT AAAGTCTTTT CTGGGCATAT TTGATGGTAT TTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68412
    Clone ID Related Accession (Same CDS sequence) XM_025288290.1 , XM_025288292.1 , XM_025288291.1 , XM_025288293.1
    Accession Version XM_025288290.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCATGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA CATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCTGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CGCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATGTACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCTAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACTG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCTGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCAC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATACAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTAATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAGATCTTA GCATAAATCT AATCAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGGCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GCAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAGAAGTC AGAAGTGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AACAAATCTC CAAATGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTGTAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACAATCAT CTGCATATCA AGACACAGGT
    AAATCTGCAT ATCAAGACAA AGAAGTTTTG AAAGTGAACT TTGAATTGGG AAATGAAGTT
    TGGGATGACT TTGATGATGA AAACTTAGTA GAAATTACTA GCTTTTCAGC TGAAACTGAG
    AAGACAAAAA CATCAGGATT TGGAAACACT TTGAGTTCAA GTACTGGGGA AAGTAAGCTA
    CCATTCCAAG AATCAAAAGG CCAATTCCAA AGAGAATTGT CTAAGAGTTT TGTTTCATCA
    CATGAAAAGC CAGATATTTA TTTATCAAAT TCTGCTGTGT CCAAGTTTGG TGCATCCTCC
    ATGACACAAT TACCTCAACA ATCTGGAAAT GCAAGTATTA TAAGAAGAAA CACTTCTCTC
    TTTTTTCTGT TAAATACTGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025144075.1
    CDS1897..6060
    Translation

    Target ORF information:

    RefSeq Version XM_025288290.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025288290.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCATGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA CATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCTGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CGCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATGTACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCTAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACTG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCTGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCAC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATACAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTAATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAGATCTTA GCATAAATCT AATCAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGGCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GCAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAGAAGTC AGAAGTGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AACAAATCTC CAAATGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTGTAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACAATCAT CTGCATATCA AGACACAGGT
    AAATCTGCAT ATCAAGACAA AGAAGTTTTG AAAGTGAACT TTGAATTGGG AAATGAAGTT
    TGGGATGACT TTGATGATGA AAACTTAGTA GAAATTACTA GCTTTTCAGC TGAAACTGAG
    AAGACAAAAA CATCAGGATT TGGAAACACT TTGAGTTCAA GTACTGGGGA AAGTAAGCTA
    CCATTCCAAG AATCAAAAGG CCAATTCCAA AGAGAATTGT CTAAGAGTTT TGTTTCATCA
    CATGAAAAGC CAGATATTTA TTTATCAAAT TCTGCTGTGT CCAAGTTTGG TGCATCCTCC
    ATGACACAAT TACCTCAACA ATCTGGAAAT GCAAGTATTA TAAGAAGAAA CACTTCTCTC
    TTTTTTCTGT TAAATACTGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68412
    Clone ID Related Accession (Same CDS sequence) XM_025288290.1 , XM_025288292.1 , XM_025288291.1 , XM_025288293.1
    Accession Version XM_025288292.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCATGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA CATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCTGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CGCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATGTACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCTAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACTG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCTGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCAC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATACAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTAATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAGATCTTA GCATAAATCT AATCAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGGCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GCAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAGAAGTC AGAAGTGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AACAAATCTC CAAATGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTGTAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACAATCAT CTGCATATCA AGACACAGGT
    AAATCTGCAT ATCAAGACAA AGAAGTTTTG AAAGTGAACT TTGAATTGGG AAATGAAGTT
    TGGGATGACT TTGATGATGA AAACTTAGTA GAAATTACTA GCTTTTCAGC TGAAACTGAG
    AAGACAAAAA CATCAGGATT TGGAAACACT TTGAGTTCAA GTACTGGGGA AAGTAAGCTA
    CCATTCCAAG AATCAAAAGG CCAATTCCAA AGAGAATTGT CTAAGAGTTT TGTTTCATCA
    CATGAAAAGC CAGATATTTA TTTATCAAAT TCTGCTGTGT CCAAGTTTGG TGCATCCTCC
    ATGACACAAT TACCTCAACA ATCTGGAAAT GCAAGTATTA TAAGAAGAAA CACTTCTCTC
    TTTTTTCTGT TAAATACTGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025144077.1
    CDS158..4321
    Translation

    Target ORF information:

    RefSeq Version XM_025288292.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025288292.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCATGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA CATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCTGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CGCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATGTACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCTAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACTG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCTGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCAC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATACAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTAATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAGATCTTA GCATAAATCT AATCAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGGCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GCAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAGAAGTC AGAAGTGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AACAAATCTC CAAATGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTGTAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACAATCAT CTGCATATCA AGACACAGGT
    AAATCTGCAT ATCAAGACAA AGAAGTTTTG AAAGTGAACT TTGAATTGGG AAATGAAGTT
    TGGGATGACT TTGATGATGA AAACTTAGTA GAAATTACTA GCTTTTCAGC TGAAACTGAG
    AAGACAAAAA CATCAGGATT TGGAAACACT TTGAGTTCAA GTACTGGGGA AAGTAAGCTA
    CCATTCCAAG AATCAAAAGG CCAATTCCAA AGAGAATTGT CTAAGAGTTT TGTTTCATCA
    CATGAAAAGC CAGATATTTA TTTATCAAAT TCTGCTGTGT CCAAGTTTGG TGCATCCTCC
    ATGACACAAT TACCTCAACA ATCTGGAAAT GCAAGTATTA TAAGAAGAAA CACTTCTCTC
    TTTTTTCTGT TAAATACTGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68412
    Clone ID Related Accession (Same CDS sequence) XM_025288290.1 , XM_025288292.1 , XM_025288291.1 , XM_025288293.1
    Accession Version XM_025288291.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCATGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA CATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCTGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CGCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATGTACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCTAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACTG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCTGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCAC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATACAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTAATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAGATCTTA GCATAAATCT AATCAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGGCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GCAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAGAAGTC AGAAGTGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AACAAATCTC CAAATGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTGTAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACAATCAT CTGCATATCA AGACACAGGT
    AAATCTGCAT ATCAAGACAA AGAAGTTTTG AAAGTGAACT TTGAATTGGG AAATGAAGTT
    TGGGATGACT TTGATGATGA AAACTTAGTA GAAATTACTA GCTTTTCAGC TGAAACTGAG
    AAGACAAAAA CATCAGGATT TGGAAACACT TTGAGTTCAA GTACTGGGGA AAGTAAGCTA
    CCATTCCAAG AATCAAAAGG CCAATTCCAA AGAGAATTGT CTAAGAGTTT TGTTTCATCA
    CATGAAAAGC CAGATATTTA TTTATCAAAT TCTGCTGTGT CCAAGTTTGG TGCATCCTCC
    ATGACACAAT TACCTCAACA ATCTGGAAAT GCAAGTATTA TAAGAAGAAA CACTTCTCTC
    TTTTTTCTGT TAAATACTGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025144076.1
    CDS771..4934
    Translation

    Target ORF information:

    RefSeq Version XM_025288291.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025288291.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCATGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA CATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCTGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CGCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATGTACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCTAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACTG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCTGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCAC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATACAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTAATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAGATCTTA GCATAAATCT AATCAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGGCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GCAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAGAAGTC AGAAGTGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AACAAATCTC CAAATGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTGTAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACAATCAT CTGCATATCA AGACACAGGT
    AAATCTGCAT ATCAAGACAA AGAAGTTTTG AAAGTGAACT TTGAATTGGG AAATGAAGTT
    TGGGATGACT TTGATGATGA AAACTTAGTA GAAATTACTA GCTTTTCAGC TGAAACTGAG
    AAGACAAAAA CATCAGGATT TGGAAACACT TTGAGTTCAA GTACTGGGGA AAGTAAGCTA
    CCATTCCAAG AATCAAAAGG CCAATTCCAA AGAGAATTGT CTAAGAGTTT TGTTTCATCA
    CATGAAAAGC CAGATATTTA TTTATCAAAT TCTGCTGTGT CCAAGTTTGG TGCATCCTCC
    ATGACACAAT TACCTCAACA ATCTGGAAAT GCAAGTATTA TAAGAAGAAA CACTTCTCTC
    TTTTTTCTGT TAAATACTGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68412
    Clone ID Related Accession (Same CDS sequence) XM_025288290.1 , XM_025288292.1 , XM_025288291.1 , XM_025288293.1
    Accession Version XM_025288293.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product probable ATP-dependent DNA helicase HFM1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCATGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA CATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCTGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CGCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATGTACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCTAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACTG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCTGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCAC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATACAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTAATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAGATCTTA GCATAAATCT AATCAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGGCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GCAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAGAAGTC AGAAGTGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AACAAATCTC CAAATGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTGTAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACAATCAT CTGCATATCA AGACACAGGT
    AAATCTGCAT ATCAAGACAA AGAAGTTTTG AAAGTGAACT TTGAATTGGG AAATGAAGTT
    TGGGATGACT TTGATGATGA AAACTTAGTA GAAATTACTA GCTTTTCAGC TGAAACTGAG
    AAGACAAAAA CATCAGGATT TGGAAACACT TTGAGTTCAA GTACTGGGGA AAGTAAGCTA
    CCATTCCAAG AATCAAAAGG CCAATTCCAA AGAGAATTGT CTAAGAGTTT TGTTTCATCA
    CATGAAAAGC CAGATATTTA TTTATCAAAT TCTGCTGTGT CCAAGTTTGG TGCATCCTCC
    ATGACACAAT TACCTCAACA ATCTGGAAAT GCAAGTATTA TAAGAAGAAA CACTTCTCTC
    TTTTTTCTGT TAAATACTGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025144078.1
    CDS1897..6060
    Translation

    Target ORF information:

    RefSeq Version XM_025288293.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025288293.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCATGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA CATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCTGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CGCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATGTACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCTAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACTG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCTGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCAC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATACAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTAATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAGATCTTA GCATAAATCT AATCAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGGCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GCAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAGAAGTC AGAAGTGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AACAAATCTC CAAATGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTGTAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACAATCAT CTGCATATCA AGACACAGGT
    AAATCTGCAT ATCAAGACAA AGAAGTTTTG AAAGTGAACT TTGAATTGGG AAATGAAGTT
    TGGGATGACT TTGATGATGA AAACTTAGTA GAAATTACTA GCTTTTCAGC TGAAACTGAG
    AAGACAAAAA CATCAGGATT TGGAAACACT TTGAGTTCAA GTACTGGGGA AAGTAAGCTA
    CCATTCCAAG AATCAAAAGG CCAATTCCAA AGAGAATTGT CTAAGAGTTT TGTTTCATCA
    CATGAAAAGC CAGATATTTA TTTATCAAAT TCTGCTGTGT CCAAGTTTGG TGCATCCTCC
    ATGACACAAT TACCTCAACA ATCTGGAAAT GCAAGTATTA TAAGAAGAAA CACTTCTCTC
    TTTTTTCTGT TAAATACTGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68413
    Clone ID Related Accession (Same CDS sequence) XM_025288294.1
    Accession Version XM_025288294.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product probable ATP-dependent DNA helicase HFM1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCATGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA CATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCTGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CGCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATGTACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCTAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACTG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCTGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCAC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATACAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTAATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAGATCTTA GCATAAATCT AATCAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGGCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GCAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAGAAGTC AGAAGTGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AACAAATCTC CAAATGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTGTAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACAATCAT CTGCATATCA AGACACAGAA
    GTTTTGAAAG TGAACTTTGA ATTGGGAAAT GAAGTTTGGG ATGACTTTGA TGATGAAAAC
    TTAGTAGAAA TTACTAGCTT TTCAGCTGAA ACTGAGAAGA CAAAAACATC AGGATTTGGA
    AACACTTTGA GTTCAAGTAC TGGGGAAAGT AAGCTACCAT TCCAAGAATC AAAAGGCCAA
    TTCCAAAGAG AATTGTCTAA GAGTTTTGTT TCATCACATG AAAAGCCAGA TATTTATTTA
    TCAAATTCTG CTGTGTCCAA GTTTGGTGCA TCCTCCATGA CACAATTACC TCAACAATCT
    GGAAATGCAA GTATTATAAG AAGAAACACT TCTCTCTTTT TTCTGTTAAA TACTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025144079.1
    CDS1897..6036
    Translation

    Target ORF information:

    RefSeq Version XM_025288294.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025288294.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCATGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA CATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCTGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CGCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATGTACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCTAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACTG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCTGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCAC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATACAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTAATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAGATCTTA GCATAAATCT AATCAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGGCCCAAA AAAGGAATGA CTGCTTGCAA AAAACCTGGG
    AACCGAGAAT GCAATCATCA CTGTAAAAAT AAACATACAT GTGGACATGA CTGCTGTAAA
    ATTGGAGTTG CACAGAAGTC AGAAGTGAAA GAATCAACAA TTTCTTCATA TTTATCTGAT
    TTAAGAAATA GAAATGCTGT TTCATCTCTT CCTCCAGTTA AACGTCTGAA GATGCAGATG
    AACAAATCTC CAAATGTGGA CCTTAAGGAC TTTGGTTTTG CTCCAAAACC TTCTCTTCCC
    AGTATAGCAA GGTCAGAATA TTTAAACACA CCTGAATTGC CTGTAATGGA GCAGAGGGAT
    CAGCATGAGA TCTATACAAA AAGTCAACAA CAACAATCAT CTGCATATCA AGACACAGAA
    GTTTTGAAAG TGAACTTTGA ATTGGGAAAT GAAGTTTGGG ATGACTTTGA TGATGAAAAC
    TTAGTAGAAA TTACTAGCTT TTCAGCTGAA ACTGAGAAGA CAAAAACATC AGGATTTGGA
    AACACTTTGA GTTCAAGTAC TGGGGAAAGT AAGCTACCAT TCCAAGAATC AAAAGGCCAA
    TTCCAAAGAG AATTGTCTAA GAGTTTTGTT TCATCACATG AAAAGCCAGA TATTTATTTA
    TCAAATTCTG CTGTGTCCAA GTTTGGTGCA TCCTCCATGA CACAATTACC TCAACAATCT
    GGAAATGCAA GTATTATAAG AAGAAACACT TCTCTCTTTT TTCTGTTAAA TACTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68414
    Clone ID Related Accession (Same CDS sequence) XM_025288296.1
    Accession Version XM_025288296.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product probable ATP-dependent DNA helicase HFM1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGATTGCAGA ATGGCTTTCA
    GATGGTGAAA GATCTGCTGT ATGTCTGAAA ATGGATGAGA GACATAGACC AGTGAAACTT
    CGGAAAGTGG TTCTTGGGTT TCCCTGCAGT GATAACCAAA CTGAATTCAA GTTTGATTTA
    ACGCTCAACT ACAAAATTGC CAGTATTATA CAAACGTACT CTGATCAGAA ACCCACACTT
    GTGTTTTGTG CAACAAGGAA AGGTGTGCAA CAGGCTGCTT CGGTTCTTGT GAAAGATGCA
    AAATTTATTA TGACTGTGGA ACAGAAACAG AGGTTACAAA AGTGTGCATA TTCCATAAAA
    GATTCAAAAC TGAGAGATAT CTTAATACAT GGTGTTGCTT ATCATCATGC TGGTATGGAA
    CTGTCAGATA GAAAAGTAGT TGAAGGAGCT TTTACTGTTG GAGATGTACC AGTTCTTTTT
    ACTACCAGTA CCTTAGCCAT GGGAGTCAAT TTGCCTGCTC ACCTAGTGGT TATAAAATCT
    ACAATGCATT ATGCTGGAGG AATGTTTGAA GAATACAGTG AAACAGATAT TCTACAGATG
    ATTGGTAGAG CTGGTCGACC TCAATTTGAC ACTACAGCTA CTGCAGTTAT TATGACTCGG
    TTAAGCACAA AAGAGAAGTA TATTCAGATG CTTGCTTGCA GTGACACTAT AGAAAGCAGT
    TTGCACAGAC ATCTTATTGA GCATTTAAAT GCAGAGATAG TATTGCATAC CATCACAAAC
    GTGAATATTG CTTTGGAATG GATACGATCA ACCTTGCTTT ATATCAGAGC CTTGAAAAAT
    CCATCTCATT ATGGTTTTGC ATCTGGATTG AACAAAGATG GAATTGAATC AAAATTACAA
    GAATTGTGTT TGAAGAATCT GAATGATTTG TCATCTCTGA ACCTGATAAA AATGGATGAA
    GATATTAATT TCAAACCAAC TGAGGCAGGA AGATTGATGG CTTGGTATTA TATTACATTT
    GAGACAGTGA AGAAATTTTG TACAATCAGT GGAAAAGAAA CTTTATCAGA TCTGGTCACA
    ATGATAGCCA GCTGCAAGGA ATTTCTAGAT GTACAATTAA GAATAAATGA AAAGAAAGCA
    TTGAATACTT TGAACAAAGA TCCAAATCGG ACAACTATCA GATTTCCAAT GGAAGGAAGG
    ATTAAAACAA GAGAAATGAA AGTAAATTGT CTTATTCAGG CTCAGCTAGG ATGCATTCCC
    GTACAAGACT TTGCTTTGAC GCAAGATACT GCAAAGATTT TCAGAAATGG CTCACGAATT
    ACAAGATGGT TATCAGATTT TGTGGCTACT CAAGAAAAGT TTGCTGTATT ATTGAACAGT
    TTGATTTTAG CTAAATGTTT TAGATGTAAA CTTTGGGAAA ACTCTCCACA TGTATCCAAA
    CAGCTGGAAA AAATTGGTAT AACATTATCA AATGCTATGG TAAATGCAGG TTTGACTTCC
    TTTAAAAAAA TAGAAGAAAC AGATGCAAGG GAACTTGAGC TGATTTTAAA TAGACATCCA
    CCTTTTGGAA CTCAGATAAA AGAAACAGTG ATGTATCTAC CAAAATATGA ACTTGAAGTG
    GAACAGATTA CAAGATACAG TGATACCATG GCAGAAATAT TAGTGACTGT TATATTAAGA
    AACTTTGAAC AGCTACAAAC TAAAAGAACA GCATCAAATT CTCACTATGT CACCTTAATC
    ATAGGTGATG CAGATAATCA ACTGGTTTTT AAGCACAAAA TTATGGATTC TGTTTTGCTA
    AAAGCTGGAA ATTGGGCTAA AAAGTTTGAT GTGAGGAGAG CTCTTAAATC TGAAGATCTT
    AGCATAAATC TAATCAGTTC AGAATATGTT GGACTTGATA TACAGCGCAA ATTTACAGTC
    TTTTACTCAG GACCCAAGAG ATTTGGAAAT CAAATAATTA TACAAAAAAA ATCAGAAACA
    GAGATTTCCC ATTCTGAACA TTCAGATAGA TCTACTGTAG CAGGGCCCAA AAAAGGAATG
    ACTGCTTGCA AAAAACCTGG GAACCGAGAA TGCAATCATC ACTGTAAAAA TAAACATACA
    TGTGGACATG ACTGCTGTAA AATTGGAGTT GCACAGAAGT CAGAAGTGAA AGAATCAACA
    ATTTCTTCAT ATTTATCTGA TTTAAGAAAT AGAAATGCTG TTTCATCTCT TCCTCCAGTT
    AAACGTCTGA AGATGCAGAT GAACAAATCT CCAAATGTGG ACCTTAAGGA CTTTGGTTTT
    GCTCCAAAAC CTTCTCTTCC CAGTATAGCA AGGTCAGAAT ATTTAAACAC ACCTGAATTG
    CCTGTAATGG AGCAGAGGGA TCAGCATGAG ATCTATACAA AAAGTCAACA ACAACAATCA
    TCTGCATATC AAGACACAGG TAAATCTGCA TATCAAGACA AAGAAGTTTT GAAAGTGAAC
    TTTGAATTGG GAAATGAAGT TTGGGATGAC TTTGATGATG AAAACTTAGT AGAAATTACT
    AGCTTTTCAG CTGAAACTGA GAAGACAAAA ACATCAGGAT TTGGAAACAC TTTGAGTTCA
    AGTACTGGGG AAAGTAAGCT ACCATTCCAA GAATCAAAAG GCCAATTCCA AAGAGAATTG
    TCTAAGAGTT TTGTTTCATC ACATGAAAAG CCAGATATTT ATTTATCAAA TTCTGCTGTG
    TCCAAGTTTG GTGCATCCTC CATGACACAA TTACCTCAAC AATCTGGAAA TGCAAGTATT
    ATAAGAAGAA ACACTTCTCT CTTTTTTCTG TTAAATACTG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025144081.1
    CDS1898..5902
    Translation

    Target ORF information:

    RefSeq Version XM_025288296.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025288296.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGATTGCAGA ATGGCTTTCA
    GATGGTGAAA GATCTGCTGT ATGTCTGAAA ATGGATGAGA GACATAGACC AGTGAAACTT
    CGGAAAGTGG TTCTTGGGTT TCCCTGCAGT GATAACCAAA CTGAATTCAA GTTTGATTTA
    ACGCTCAACT ACAAAATTGC CAGTATTATA CAAACGTACT CTGATCAGAA ACCCACACTT
    GTGTTTTGTG CAACAAGGAA AGGTGTGCAA CAGGCTGCTT CGGTTCTTGT GAAAGATGCA
    AAATTTATTA TGACTGTGGA ACAGAAACAG AGGTTACAAA AGTGTGCATA TTCCATAAAA
    GATTCAAAAC TGAGAGATAT CTTAATACAT GGTGTTGCTT ATCATCATGC TGGTATGGAA
    CTGTCAGATA GAAAAGTAGT TGAAGGAGCT TTTACTGTTG GAGATGTACC AGTTCTTTTT
    ACTACCAGTA CCTTAGCCAT GGGAGTCAAT TTGCCTGCTC ACCTAGTGGT TATAAAATCT
    ACAATGCATT ATGCTGGAGG AATGTTTGAA GAATACAGTG AAACAGATAT TCTACAGATG
    ATTGGTAGAG CTGGTCGACC TCAATTTGAC ACTACAGCTA CTGCAGTTAT TATGACTCGG
    TTAAGCACAA AAGAGAAGTA TATTCAGATG CTTGCTTGCA GTGACACTAT AGAAAGCAGT
    TTGCACAGAC ATCTTATTGA GCATTTAAAT GCAGAGATAG TATTGCATAC CATCACAAAC
    GTGAATATTG CTTTGGAATG GATACGATCA ACCTTGCTTT ATATCAGAGC CTTGAAAAAT
    CCATCTCATT ATGGTTTTGC ATCTGGATTG AACAAAGATG GAATTGAATC AAAATTACAA
    GAATTGTGTT TGAAGAATCT GAATGATTTG TCATCTCTGA ACCTGATAAA AATGGATGAA
    GATATTAATT TCAAACCAAC TGAGGCAGGA AGATTGATGG CTTGGTATTA TATTACATTT
    GAGACAGTGA AGAAATTTTG TACAATCAGT GGAAAAGAAA CTTTATCAGA TCTGGTCACA
    ATGATAGCCA GCTGCAAGGA ATTTCTAGAT GTACAATTAA GAATAAATGA AAAGAAAGCA
    TTGAATACTT TGAACAAAGA TCCAAATCGG ACAACTATCA GATTTCCAAT GGAAGGAAGG
    ATTAAAACAA GAGAAATGAA AGTAAATTGT CTTATTCAGG CTCAGCTAGG ATGCATTCCC
    GTACAAGACT TTGCTTTGAC GCAAGATACT GCAAAGATTT TCAGAAATGG CTCACGAATT
    ACAAGATGGT TATCAGATTT TGTGGCTACT CAAGAAAAGT TTGCTGTATT ATTGAACAGT
    TTGATTTTAG CTAAATGTTT TAGATGTAAA CTTTGGGAAA ACTCTCCACA TGTATCCAAA
    CAGCTGGAAA AAATTGGTAT AACATTATCA AATGCTATGG TAAATGCAGG TTTGACTTCC
    TTTAAAAAAA TAGAAGAAAC AGATGCAAGG GAACTTGAGC TGATTTTAAA TAGACATCCA
    CCTTTTGGAA CTCAGATAAA AGAAACAGTG ATGTATCTAC CAAAATATGA ACTTGAAGTG
    GAACAGATTA CAAGATACAG TGATACCATG GCAGAAATAT TAGTGACTGT TATATTAAGA
    AACTTTGAAC AGCTACAAAC TAAAAGAACA GCATCAAATT CTCACTATGT CACCTTAATC
    ATAGGTGATG CAGATAATCA ACTGGTTTTT AAGCACAAAA TTATGGATTC TGTTTTGCTA
    AAAGCTGGAA ATTGGGCTAA AAAGTTTGAT GTGAGGAGAG CTCTTAAATC TGAAGATCTT
    AGCATAAATC TAATCAGTTC AGAATATGTT GGACTTGATA TACAGCGCAA ATTTACAGTC
    TTTTACTCAG GACCCAAGAG ATTTGGAAAT CAAATAATTA TACAAAAAAA ATCAGAAACA
    GAGATTTCCC ATTCTGAACA TTCAGATAGA TCTACTGTAG CAGGGCCCAA AAAAGGAATG
    ACTGCTTGCA AAAAACCTGG GAACCGAGAA TGCAATCATC ACTGTAAAAA TAAACATACA
    TGTGGACATG ACTGCTGTAA AATTGGAGTT GCACAGAAGT CAGAAGTGAA AGAATCAACA
    ATTTCTTCAT ATTTATCTGA TTTAAGAAAT AGAAATGCTG TTTCATCTCT TCCTCCAGTT
    AAACGTCTGA AGATGCAGAT GAACAAATCT CCAAATGTGG ACCTTAAGGA CTTTGGTTTT
    GCTCCAAAAC CTTCTCTTCC CAGTATAGCA AGGTCAGAAT ATTTAAACAC ACCTGAATTG
    CCTGTAATGG AGCAGAGGGA TCAGCATGAG ATCTATACAA AAAGTCAACA ACAACAATCA
    TCTGCATATC AAGACACAGG TAAATCTGCA TATCAAGACA AAGAAGTTTT GAAAGTGAAC
    TTTGAATTGG GAAATGAAGT TTGGGATGAC TTTGATGATG AAAACTTAGT AGAAATTACT
    AGCTTTTCAG CTGAAACTGA GAAGACAAAA ACATCAGGAT TTGGAAACAC TTTGAGTTCA
    AGTACTGGGG AAAGTAAGCT ACCATTCCAA GAATCAAAAG GCCAATTCCA AAGAGAATTG
    TCTAAGAGTT TTGTTTCATC ACATGAAAAG CCAGATATTT ATTTATCAAA TTCTGCTGTG
    TCCAAGTTTG GTGCATCCTC CATGACACAA TTACCTCAAC AATCTGGAAA TGCAAGTATT
    ATAAGAAGAA ACACTTCTCT CTTTTTTCTG TTAAATACTG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68415
    Clone ID Related Accession (Same CDS sequence) XM_025288297.1 , XM_025288298.1
    Accession Version XM_025288297.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3960bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product probable ATP-dependent DNA helicase HFM1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTACCAT CAAATTTAAA GATAACTAAT GAAGACACAA ATTATGTTTC ACAAACCCAA 
    AAATTCCAGT TTAACTTCCC TTCTAATGAA TATGAAGAAG ATGATCTAAA TTTAGGAGGG
    GCAGATAACA ATGACTTACA TGTTGCTGGC AAGCTGACAT GTGGTTCTTC TCAGAAATGC
    AGAAATCACA TTGGCACTGA GATAGAACTT GAGAAAAATG TTCCAGATGA TACAAAGTTA
    GTTAATTCTG CACAAGATAA AGGAGTGAGC ATGTCAGCCT TCCGCAAAAG GTTATTTAAA
    ATATCTGATA ATATACATGA GGATGCTTAT TCCAATGACA GTGCAAACTT GGACTCTAAT
    ATTGGCTCAG TGAAAATTGT CCAAACAGAA GTGAACAAAG GGAAATCATG GAAAGATAGC
    AATAGTAAGC AGAAACCTCA ATATTCAGAC TGTAACATTT TGACAGCCAC TGATACACTT
    TTTGCTTCTG AAATCGGAGA AGGCATAATC AAAGCACCAC CTTTTCCAGT TGCATCCCAG
    CCTCATACAG TTCAAGGGGT AACAGAAAGA AGTTTAGATT CCTTGAAGGC TGTCACAGAA
    ATTCCTGCTA AATTTAGAAG TATTTTCAAA GAATTCCCAT ATTTCAATTA TATACAGTCC
    AAAGCCTTTG ATGATCTTCT TTATACAGAT AGGAATTTTG TGATTTGTGC TCCAACTGGT
    TCTGGGAAAA CTGTAGTGTT TGAACTAGCT ATAACAAGAT TGTTAATGGA AGTACCATTG
    CCATGGTCAA ACATTAAAAT TGTTTACATG GCACCAATAA AAGCCCTGTG TAGTCAGCGT
    TTTGATGATT GGAAAAAAAA ATTTGGACCA ATAGGATTGA ATTGTAAAGA ACTCACTGGA
    GATACAGTAA TGGATGACCT ATTTGAGATT CAGCATGCCC ATATTATTAT GACAACTCCA
    GAAAAATGGG ATAGCATGAC TAGAAAATGG AGAGACAACT CTTTGGTCCA TCTGGTTCGA
    TTGTTTCTCA TTGATGAGGT ACATGTTGTA AAAGATGAAA ATCGTGGACC AACTCTTGAA
    GTTGTAGTCA GCAGAATGAA AACTGTGCAG ATTTTATCTC ATGCTGTAGA AAATACCAAC
    AGTATTATTC CAATGAGATT TGTGGCTGTA TCTGCAACAA TTCCAAATGC TGAGGACATT
    GCAGAATGGC TTTCAGATGG TGAAAGATCT GCTGTATGTC TGAAAATGGA TGAGAGACAT
    AGACCAGTGA AACTTCGGAA AGTGGTTCTT GGGTTTCCCT GCAGTGATAA CCAAACTGAA
    TTCAAGTTTG ATTTAACGCT CAACTACAAA ATTGCCAGTA TTATACAAAC GTACTCTGAT
    CAGAAACCCA CACTTGTGTT TTGTGCAACA AGGAAAGGTG TGCAACAGGC TGCTTCGGTT
    CTTGTGAAAG ATGCAAAATT TATTATGACT GTGGAACAGA AACAGAGGTT ACAAAAGTGT
    GCATATTCCA TAAAAGATTC AAAACTGAGA GATATCTTAA TACATGGTGT TGCTTATCAT
    CATGCTGGTA TGGAACTGTC AGATAGAAAA GTAGTTGAAG GAGCTTTTAC TGTTGGAGAT
    GTACCAGTTC TTTTTACTAC CAGTACCTTA GCCATGGGAG TCAATTTGCC TGCTCACCTA
    GTGGTTATAA AATCTACAAT GCATTATGCT GGAGGAATGT TTGAAGAATA CAGTGAAACA
    GATATTCTAC AGATGATTGG TAGAGCTGGT CGACCTCAAT TTGACACTAC AGCTACTGCA
    GTTATTATGA CTCGGTTAAG CACAAAAGAG AAGTATATTC AGATGCTTGC TTGCAGTGAC
    ACTATAGAAA GCAGTTTGCA CAGACATCTT ATTGAGCATT TAAATGCAGA GATAGTATTG
    CATACCATCA CAAACGTGAA TATTGCTTTG GAATGGATAC GATCAACCTT GCTTTATATC
    AGAGCCTTGA AAAATCCATC TCATTATGGT TTTGCATCTG GATTGAACAA AGATGGAATT
    GAATCAAAAT TACAAGAATT GTGTTTGAAG AATCTGAATG ATTTGTCATC TCTGAACCTG
    ATAAAAATGG ATGAAGATAT TAATTTCAAA CCAACTGAGG CAGGAAGATT GATGGCTTGG
    TATTATATTA CATTTGAGAC AGTGAAGAAA TTTTGTACAA TCAGTGGAAA AGAAACTTTA
    TCAGATCTGG TCACAATGAT AGCCAGCTGC AAGGAATTTC TAGATGTACA ATTAAGAATA
    AATGAAAAGA AAGCATTGAA TACTTTGAAC AAAGATCCAA ATCGGACAAC TATCAGATTT
    CCAATGGAAG GAAGGATTAA AACAAGAGAA ATGAAAGTAA ATTGTCTTAT TCAGGCTCAG
    CTAGGATGCA TTCCCGTACA AGACTTTGCT TTGACGCAAG ATACTGCAAA GATTTTCAGA
    AATGGCTCAC GAATTACAAG ATGGTTATCA GATTTTGTGG CTACTCAAGA AAAGTTTGCT
    GTATTATTGA ACAGTTTGAT TTTAGCTAAA TGTTTTAGAT GTAAACTTTG GGAAAACTCT
    CCACATGTAT CCAAACAGCT GGAAAAAATT GGTATAACAT TATCAAATGC TATGGTAAAT
    GCAGGTTTGA CTTCCTTTAA AAAAATAGAA GAAACAGATG CAAGGGAACT TGAGCTGATT
    TTAAATAGAC ATCCACCTTT TGGAACTCAG ATAAAAGAAA CAGTGATGTA TCTACCAAAA
    TATGAACTTG AAGTGGAACA GATTACAAGA TACAGTGATA CCATGGCAGA AATATTAGTG
    ACTGTTATAT TAAGAAACTT TGAACAGCTA CAAACTAAAA GAACAGCATC AAATTCTCAC
    TATGTCACCT TAATCATAGG TGATGCAGAT AATCAACTGG TTTTTAAGCA CAAAATTATG
    GATTCTGTTT TGCTAAAAGC TGGAAATTGG GCTAAAAAGT TTGATGTGAG GAGAGCTCTT
    AAATCTGAAG ATCTTAGCAT AAATCTAATC AGTTCAGAAT ATGTTGGACT TGATATACAG
    CGCAAATTTA CAGTCTTTTA CTCAGGACCC AAGAGATTTG GAAATCAAAT AATTATACAA
    AAAAAATCAG AAACAGAGAT TTCCCATTCT GAACATTCAG ATAGATCTAC TGTAGCAGGG
    CCCAAAAAAG GAATGACTGC TTGCAAAAAA CCTGGGAACC GAGAATGCAA TCATCACTGT
    AAAAATAAAC ATACATGTGG ACATGACTGC TGTAAAATTG GAGTTGCACA GAAGTCAGAA
    GTGAAAGAAT CAACAATTTC TTCATATTTA TCTGATTTAA GAAATAGAAA TGCTGTTTCA
    TCTCTTCCTC CAGTTAAACG TCTGAAGATG CAGATGAACA AATCTCCAAA TGTGGACCTT
    AAGGACTTTG GTTTTGCTCC AAAACCTTCT CTTCCCAGTA TAGCAAGGTC AGAATATTTA
    AACACACCTG AATTGCCTGT AATGGAGCAG AGGGATCAGC ATGAGATCTA TACAAAAAGT
    CAACAACAAC AATCATCTGC ATATCAAGAC ACAGGTAAAT CTGCATATCA AGACAAAGAA
    GTTTTGAAAG TGAACTTTGA ATTGGGAAAT GAAGTTTGGG ATGACTTTGA TGATGAAAAC
    TTAGTAGAAA TTACTAGCTT TTCAGCTGAA ACTGAGAAGA CAAAAACATC AGGATTTGGA
    AACACTTTGA GTTCAAGTAC TGGGGAAAGT AAGCTACCAT TCCAAGAATC AAAAGGCCAA
    TTCCAAAGAG AATTGTCTAA GAGTTTTGTT TCATCACATG AAAAGCCAGA TATTTATTTA
    TCAAATTCTG CTGTGTCCAA GTTTGGTGCA TCCTCCATGA CACAATTACC TCAACAATCT
    GGAAATGCAA GTATTATAAG AAGAAACACT TCTCTCTTTT TTCTGTTAAA TACTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025144082.1
    CDS764..4723
    Translation

    Target ORF information:

    RefSeq Version XM_025288297.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025288297.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTACCAT CAAATTTAAA GATAACTAAT GAAGACACAA ATTATGTTTC ACAAACCCAA 
    AAATTCCAGT TTAACTTCCC TTCTAATGAA TATGAAGAAG ATGATCTAAA TTTAGGAGGG
    GCAGATAACA ATGACTTACA TGTTGCTGGC AAGCTGACAT GTGGTTCTTC TCAGAAATGC
    AGAAATCACA TTGGCACTGA GATAGAACTT GAGAAAAATG TTCCAGATGA TACAAAGTTA
    GTTAATTCTG CACAAGATAA AGGAGTGAGC ATGTCAGCCT TCCGCAAAAG GTTATTTAAA
    ATATCTGATA ATATACATGA GGATGCTTAT TCCAATGACA GTGCAAACTT GGACTCTAAT
    ATTGGCTCAG TGAAAATTGT CCAAACAGAA GTGAACAAAG GGAAATCATG GAAAGATAGC
    AATAGTAAGC AGAAACCTCA ATATTCAGAC TGTAACATTT TGACAGCCAC TGATACACTT
    TTTGCTTCTG AAATCGGAGA AGGCATAATC AAAGCACCAC CTTTTCCAGT TGCATCCCAG
    CCTCATACAG TTCAAGGGGT AACAGAAAGA AGTTTAGATT CCTTGAAGGC TGTCACAGAA
    ATTCCTGCTA AATTTAGAAG TATTTTCAAA GAATTCCCAT ATTTCAATTA TATACAGTCC
    AAAGCCTTTG ATGATCTTCT TTATACAGAT AGGAATTTTG TGATTTGTGC TCCAACTGGT
    TCTGGGAAAA CTGTAGTGTT TGAACTAGCT ATAACAAGAT TGTTAATGGA AGTACCATTG
    CCATGGTCAA ACATTAAAAT TGTTTACATG GCACCAATAA AAGCCCTGTG TAGTCAGCGT
    TTTGATGATT GGAAAAAAAA ATTTGGACCA ATAGGATTGA ATTGTAAAGA ACTCACTGGA
    GATACAGTAA TGGATGACCT ATTTGAGATT CAGCATGCCC ATATTATTAT GACAACTCCA
    GAAAAATGGG ATAGCATGAC TAGAAAATGG AGAGACAACT CTTTGGTCCA TCTGGTTCGA
    TTGTTTCTCA TTGATGAGGT ACATGTTGTA AAAGATGAAA ATCGTGGACC AACTCTTGAA
    GTTGTAGTCA GCAGAATGAA AACTGTGCAG ATTTTATCTC ATGCTGTAGA AAATACCAAC
    AGTATTATTC CAATGAGATT TGTGGCTGTA TCTGCAACAA TTCCAAATGC TGAGGACATT
    GCAGAATGGC TTTCAGATGG TGAAAGATCT GCTGTATGTC TGAAAATGGA TGAGAGACAT
    AGACCAGTGA AACTTCGGAA AGTGGTTCTT GGGTTTCCCT GCAGTGATAA CCAAACTGAA
    TTCAAGTTTG ATTTAACGCT CAACTACAAA ATTGCCAGTA TTATACAAAC GTACTCTGAT
    CAGAAACCCA CACTTGTGTT TTGTGCAACA AGGAAAGGTG TGCAACAGGC TGCTTCGGTT
    CTTGTGAAAG ATGCAAAATT TATTATGACT GTGGAACAGA AACAGAGGTT ACAAAAGTGT
    GCATATTCCA TAAAAGATTC AAAACTGAGA GATATCTTAA TACATGGTGT TGCTTATCAT
    CATGCTGGTA TGGAACTGTC AGATAGAAAA GTAGTTGAAG GAGCTTTTAC TGTTGGAGAT
    GTACCAGTTC TTTTTACTAC CAGTACCTTA GCCATGGGAG TCAATTTGCC TGCTCACCTA
    GTGGTTATAA AATCTACAAT GCATTATGCT GGAGGAATGT TTGAAGAATA CAGTGAAACA
    GATATTCTAC AGATGATTGG TAGAGCTGGT CGACCTCAAT TTGACACTAC AGCTACTGCA
    GTTATTATGA CTCGGTTAAG CACAAAAGAG AAGTATATTC AGATGCTTGC TTGCAGTGAC
    ACTATAGAAA GCAGTTTGCA CAGACATCTT ATTGAGCATT TAAATGCAGA GATAGTATTG
    CATACCATCA CAAACGTGAA TATTGCTTTG GAATGGATAC GATCAACCTT GCTTTATATC
    AGAGCCTTGA AAAATCCATC TCATTATGGT TTTGCATCTG GATTGAACAA AGATGGAATT
    GAATCAAAAT TACAAGAATT GTGTTTGAAG AATCTGAATG ATTTGTCATC TCTGAACCTG
    ATAAAAATGG ATGAAGATAT TAATTTCAAA CCAACTGAGG CAGGAAGATT GATGGCTTGG
    TATTATATTA CATTTGAGAC AGTGAAGAAA TTTTGTACAA TCAGTGGAAA AGAAACTTTA
    TCAGATCTGG TCACAATGAT AGCCAGCTGC AAGGAATTTC TAGATGTACA ATTAAGAATA
    AATGAAAAGA AAGCATTGAA TACTTTGAAC AAAGATCCAA ATCGGACAAC TATCAGATTT
    CCAATGGAAG GAAGGATTAA AACAAGAGAA ATGAAAGTAA ATTGTCTTAT TCAGGCTCAG
    CTAGGATGCA TTCCCGTACA AGACTTTGCT TTGACGCAAG ATACTGCAAA GATTTTCAGA
    AATGGCTCAC GAATTACAAG ATGGTTATCA GATTTTGTGG CTACTCAAGA AAAGTTTGCT
    GTATTATTGA ACAGTTTGAT TTTAGCTAAA TGTTTTAGAT GTAAACTTTG GGAAAACTCT
    CCACATGTAT CCAAACAGCT GGAAAAAATT GGTATAACAT TATCAAATGC TATGGTAAAT
    GCAGGTTTGA CTTCCTTTAA AAAAATAGAA GAAACAGATG CAAGGGAACT TGAGCTGATT
    TTAAATAGAC ATCCACCTTT TGGAACTCAG ATAAAAGAAA CAGTGATGTA TCTACCAAAA
    TATGAACTTG AAGTGGAACA GATTACAAGA TACAGTGATA CCATGGCAGA AATATTAGTG
    ACTGTTATAT TAAGAAACTT TGAACAGCTA CAAACTAAAA GAACAGCATC AAATTCTCAC
    TATGTCACCT TAATCATAGG TGATGCAGAT AATCAACTGG TTTTTAAGCA CAAAATTATG
    GATTCTGTTT TGCTAAAAGC TGGAAATTGG GCTAAAAAGT TTGATGTGAG GAGAGCTCTT
    AAATCTGAAG ATCTTAGCAT AAATCTAATC AGTTCAGAAT ATGTTGGACT TGATATACAG
    CGCAAATTTA CAGTCTTTTA CTCAGGACCC AAGAGATTTG GAAATCAAAT AATTATACAA
    AAAAAATCAG AAACAGAGAT TTCCCATTCT GAACATTCAG ATAGATCTAC TGTAGCAGGG
    CCCAAAAAAG GAATGACTGC TTGCAAAAAA CCTGGGAACC GAGAATGCAA TCATCACTGT
    AAAAATAAAC ATACATGTGG ACATGACTGC TGTAAAATTG GAGTTGCACA GAAGTCAGAA
    GTGAAAGAAT CAACAATTTC TTCATATTTA TCTGATTTAA GAAATAGAAA TGCTGTTTCA
    TCTCTTCCTC CAGTTAAACG TCTGAAGATG CAGATGAACA AATCTCCAAA TGTGGACCTT
    AAGGACTTTG GTTTTGCTCC AAAACCTTCT CTTCCCAGTA TAGCAAGGTC AGAATATTTA
    AACACACCTG AATTGCCTGT AATGGAGCAG AGGGATCAGC ATGAGATCTA TACAAAAAGT
    CAACAACAAC AATCATCTGC ATATCAAGAC ACAGGTAAAT CTGCATATCA AGACAAAGAA
    GTTTTGAAAG TGAACTTTGA ATTGGGAAAT GAAGTTTGGG ATGACTTTGA TGATGAAAAC
    TTAGTAGAAA TTACTAGCTT TTCAGCTGAA ACTGAGAAGA CAAAAACATC AGGATTTGGA
    AACACTTTGA GTTCAAGTAC TGGGGAAAGT AAGCTACCAT TCCAAGAATC AAAAGGCCAA
    TTCCAAAGAG AATTGTCTAA GAGTTTTGTT TCATCACATG AAAAGCCAGA TATTTATTTA
    TCAAATTCTG CTGTGTCCAA GTTTGGTGCA TCCTCCATGA CACAATTACC TCAACAATCT
    GGAAATGCAA GTATTATAAG AAGAAACACT TCTCTCTTTT TTCTGTTAAA TACTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68415
    Clone ID Related Accession (Same CDS sequence) XM_025288297.1 , XM_025288298.1
    Accession Version XM_025288298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3960bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product probable ATP-dependent DNA helicase HFM1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTACCAT CAAATTTAAA GATAACTAAT GAAGACACAA ATTATGTTTC ACAAACCCAA 
    AAATTCCAGT TTAACTTCCC TTCTAATGAA TATGAAGAAG ATGATCTAAA TTTAGGAGGG
    GCAGATAACA ATGACTTACA TGTTGCTGGC AAGCTGACAT GTGGTTCTTC TCAGAAATGC
    AGAAATCACA TTGGCACTGA GATAGAACTT GAGAAAAATG TTCCAGATGA TACAAAGTTA
    GTTAATTCTG CACAAGATAA AGGAGTGAGC ATGTCAGCCT TCCGCAAAAG GTTATTTAAA
    ATATCTGATA ATATACATGA GGATGCTTAT TCCAATGACA GTGCAAACTT GGACTCTAAT
    ATTGGCTCAG TGAAAATTGT CCAAACAGAA GTGAACAAAG GGAAATCATG GAAAGATAGC
    AATAGTAAGC AGAAACCTCA ATATTCAGAC TGTAACATTT TGACAGCCAC TGATACACTT
    TTTGCTTCTG AAATCGGAGA AGGCATAATC AAAGCACCAC CTTTTCCAGT TGCATCCCAG
    CCTCATACAG TTCAAGGGGT AACAGAAAGA AGTTTAGATT CCTTGAAGGC TGTCACAGAA
    ATTCCTGCTA AATTTAGAAG TATTTTCAAA GAATTCCCAT ATTTCAATTA TATACAGTCC
    AAAGCCTTTG ATGATCTTCT TTATACAGAT AGGAATTTTG TGATTTGTGC TCCAACTGGT
    TCTGGGAAAA CTGTAGTGTT TGAACTAGCT ATAACAAGAT TGTTAATGGA AGTACCATTG
    CCATGGTCAA ACATTAAAAT TGTTTACATG GCACCAATAA AAGCCCTGTG TAGTCAGCGT
    TTTGATGATT GGAAAAAAAA ATTTGGACCA ATAGGATTGA ATTGTAAAGA ACTCACTGGA
    GATACAGTAA TGGATGACCT ATTTGAGATT CAGCATGCCC ATATTATTAT GACAACTCCA
    GAAAAATGGG ATAGCATGAC TAGAAAATGG AGAGACAACT CTTTGGTCCA TCTGGTTCGA
    TTGTTTCTCA TTGATGAGGT ACATGTTGTA AAAGATGAAA ATCGTGGACC AACTCTTGAA
    GTTGTAGTCA GCAGAATGAA AACTGTGCAG ATTTTATCTC ATGCTGTAGA AAATACCAAC
    AGTATTATTC CAATGAGATT TGTGGCTGTA TCTGCAACAA TTCCAAATGC TGAGGACATT
    GCAGAATGGC TTTCAGATGG TGAAAGATCT GCTGTATGTC TGAAAATGGA TGAGAGACAT
    AGACCAGTGA AACTTCGGAA AGTGGTTCTT GGGTTTCCCT GCAGTGATAA CCAAACTGAA
    TTCAAGTTTG ATTTAACGCT CAACTACAAA ATTGCCAGTA TTATACAAAC GTACTCTGAT
    CAGAAACCCA CACTTGTGTT TTGTGCAACA AGGAAAGGTG TGCAACAGGC TGCTTCGGTT
    CTTGTGAAAG ATGCAAAATT TATTATGACT GTGGAACAGA AACAGAGGTT ACAAAAGTGT
    GCATATTCCA TAAAAGATTC AAAACTGAGA GATATCTTAA TACATGGTGT TGCTTATCAT
    CATGCTGGTA TGGAACTGTC AGATAGAAAA GTAGTTGAAG GAGCTTTTAC TGTTGGAGAT
    GTACCAGTTC TTTTTACTAC CAGTACCTTA GCCATGGGAG TCAATTTGCC TGCTCACCTA
    GTGGTTATAA AATCTACAAT GCATTATGCT GGAGGAATGT TTGAAGAATA CAGTGAAACA
    GATATTCTAC AGATGATTGG TAGAGCTGGT CGACCTCAAT TTGACACTAC AGCTACTGCA
    GTTATTATGA CTCGGTTAAG CACAAAAGAG AAGTATATTC AGATGCTTGC TTGCAGTGAC
    ACTATAGAAA GCAGTTTGCA CAGACATCTT ATTGAGCATT TAAATGCAGA GATAGTATTG
    CATACCATCA CAAACGTGAA TATTGCTTTG GAATGGATAC GATCAACCTT GCTTTATATC
    AGAGCCTTGA AAAATCCATC TCATTATGGT TTTGCATCTG GATTGAACAA AGATGGAATT
    GAATCAAAAT TACAAGAATT GTGTTTGAAG AATCTGAATG ATTTGTCATC TCTGAACCTG
    ATAAAAATGG ATGAAGATAT TAATTTCAAA CCAACTGAGG CAGGAAGATT GATGGCTTGG
    TATTATATTA CATTTGAGAC AGTGAAGAAA TTTTGTACAA TCAGTGGAAA AGAAACTTTA
    TCAGATCTGG TCACAATGAT AGCCAGCTGC AAGGAATTTC TAGATGTACA ATTAAGAATA
    AATGAAAAGA AAGCATTGAA TACTTTGAAC AAAGATCCAA ATCGGACAAC TATCAGATTT
    CCAATGGAAG GAAGGATTAA AACAAGAGAA ATGAAAGTAA ATTGTCTTAT TCAGGCTCAG
    CTAGGATGCA TTCCCGTACA AGACTTTGCT TTGACGCAAG ATACTGCAAA GATTTTCAGA
    AATGGCTCAC GAATTACAAG ATGGTTATCA GATTTTGTGG CTACTCAAGA AAAGTTTGCT
    GTATTATTGA ACAGTTTGAT TTTAGCTAAA TGTTTTAGAT GTAAACTTTG GGAAAACTCT
    CCACATGTAT CCAAACAGCT GGAAAAAATT GGTATAACAT TATCAAATGC TATGGTAAAT
    GCAGGTTTGA CTTCCTTTAA AAAAATAGAA GAAACAGATG CAAGGGAACT TGAGCTGATT
    TTAAATAGAC ATCCACCTTT TGGAACTCAG ATAAAAGAAA CAGTGATGTA TCTACCAAAA
    TATGAACTTG AAGTGGAACA GATTACAAGA TACAGTGATA CCATGGCAGA AATATTAGTG
    ACTGTTATAT TAAGAAACTT TGAACAGCTA CAAACTAAAA GAACAGCATC AAATTCTCAC
    TATGTCACCT TAATCATAGG TGATGCAGAT AATCAACTGG TTTTTAAGCA CAAAATTATG
    GATTCTGTTT TGCTAAAAGC TGGAAATTGG GCTAAAAAGT TTGATGTGAG GAGAGCTCTT
    AAATCTGAAG ATCTTAGCAT AAATCTAATC AGTTCAGAAT ATGTTGGACT TGATATACAG
    CGCAAATTTA CAGTCTTTTA CTCAGGACCC AAGAGATTTG GAAATCAAAT AATTATACAA
    AAAAAATCAG AAACAGAGAT TTCCCATTCT GAACATTCAG ATAGATCTAC TGTAGCAGGG
    CCCAAAAAAG GAATGACTGC TTGCAAAAAA CCTGGGAACC GAGAATGCAA TCATCACTGT
    AAAAATAAAC ATACATGTGG ACATGACTGC TGTAAAATTG GAGTTGCACA GAAGTCAGAA
    GTGAAAGAAT CAACAATTTC TTCATATTTA TCTGATTTAA GAAATAGAAA TGCTGTTTCA
    TCTCTTCCTC CAGTTAAACG TCTGAAGATG CAGATGAACA AATCTCCAAA TGTGGACCTT
    AAGGACTTTG GTTTTGCTCC AAAACCTTCT CTTCCCAGTA TAGCAAGGTC AGAATATTTA
    AACACACCTG AATTGCCTGT AATGGAGCAG AGGGATCAGC ATGAGATCTA TACAAAAAGT
    CAACAACAAC AATCATCTGC ATATCAAGAC ACAGGTAAAT CTGCATATCA AGACAAAGAA
    GTTTTGAAAG TGAACTTTGA ATTGGGAAAT GAAGTTTGGG ATGACTTTGA TGATGAAAAC
    TTAGTAGAAA TTACTAGCTT TTCAGCTGAA ACTGAGAAGA CAAAAACATC AGGATTTGGA
    AACACTTTGA GTTCAAGTAC TGGGGAAAGT AAGCTACCAT TCCAAGAATC AAAAGGCCAA
    TTCCAAAGAG AATTGTCTAA GAGTTTTGTT TCATCACATG AAAAGCCAGA TATTTATTTA
    TCAAATTCTG CTGTGTCCAA GTTTGGTGCA TCCTCCATGA CACAATTACC TCAACAATCT
    GGAAATGCAA GTATTATAAG AAGAAACACT TCTCTCTTTT TTCTGTTAAA TACTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025144083.1
    CDS151..4110
    Translation

    Target ORF information:

    RefSeq Version XM_025288298.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025288298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTTACCAT CAAATTTAAA GATAACTAAT GAAGACACAA ATTATGTTTC ACAAACCCAA 
    AAATTCCAGT TTAACTTCCC TTCTAATGAA TATGAAGAAG ATGATCTAAA TTTAGGAGGG
    GCAGATAACA ATGACTTACA TGTTGCTGGC AAGCTGACAT GTGGTTCTTC TCAGAAATGC
    AGAAATCACA TTGGCACTGA GATAGAACTT GAGAAAAATG TTCCAGATGA TACAAAGTTA
    GTTAATTCTG CACAAGATAA AGGAGTGAGC ATGTCAGCCT TCCGCAAAAG GTTATTTAAA
    ATATCTGATA ATATACATGA GGATGCTTAT TCCAATGACA GTGCAAACTT GGACTCTAAT
    ATTGGCTCAG TGAAAATTGT CCAAACAGAA GTGAACAAAG GGAAATCATG GAAAGATAGC
    AATAGTAAGC AGAAACCTCA ATATTCAGAC TGTAACATTT TGACAGCCAC TGATACACTT
    TTTGCTTCTG AAATCGGAGA AGGCATAATC AAAGCACCAC CTTTTCCAGT TGCATCCCAG
    CCTCATACAG TTCAAGGGGT AACAGAAAGA AGTTTAGATT CCTTGAAGGC TGTCACAGAA
    ATTCCTGCTA AATTTAGAAG TATTTTCAAA GAATTCCCAT ATTTCAATTA TATACAGTCC
    AAAGCCTTTG ATGATCTTCT TTATACAGAT AGGAATTTTG TGATTTGTGC TCCAACTGGT
    TCTGGGAAAA CTGTAGTGTT TGAACTAGCT ATAACAAGAT TGTTAATGGA AGTACCATTG
    CCATGGTCAA ACATTAAAAT TGTTTACATG GCACCAATAA AAGCCCTGTG TAGTCAGCGT
    TTTGATGATT GGAAAAAAAA ATTTGGACCA ATAGGATTGA ATTGTAAAGA ACTCACTGGA
    GATACAGTAA TGGATGACCT ATTTGAGATT CAGCATGCCC ATATTATTAT GACAACTCCA
    GAAAAATGGG ATAGCATGAC TAGAAAATGG AGAGACAACT CTTTGGTCCA TCTGGTTCGA
    TTGTTTCTCA TTGATGAGGT ACATGTTGTA AAAGATGAAA ATCGTGGACC AACTCTTGAA
    GTTGTAGTCA GCAGAATGAA AACTGTGCAG ATTTTATCTC ATGCTGTAGA AAATACCAAC
    AGTATTATTC CAATGAGATT TGTGGCTGTA TCTGCAACAA TTCCAAATGC TGAGGACATT
    GCAGAATGGC TTTCAGATGG TGAAAGATCT GCTGTATGTC TGAAAATGGA TGAGAGACAT
    AGACCAGTGA AACTTCGGAA AGTGGTTCTT GGGTTTCCCT GCAGTGATAA CCAAACTGAA
    TTCAAGTTTG ATTTAACGCT CAACTACAAA ATTGCCAGTA TTATACAAAC GTACTCTGAT
    CAGAAACCCA CACTTGTGTT TTGTGCAACA AGGAAAGGTG TGCAACAGGC TGCTTCGGTT
    CTTGTGAAAG ATGCAAAATT TATTATGACT GTGGAACAGA AACAGAGGTT ACAAAAGTGT
    GCATATTCCA TAAAAGATTC AAAACTGAGA GATATCTTAA TACATGGTGT TGCTTATCAT
    CATGCTGGTA TGGAACTGTC AGATAGAAAA GTAGTTGAAG GAGCTTTTAC TGTTGGAGAT
    GTACCAGTTC TTTTTACTAC CAGTACCTTA GCCATGGGAG TCAATTTGCC TGCTCACCTA
    GTGGTTATAA AATCTACAAT GCATTATGCT GGAGGAATGT TTGAAGAATA CAGTGAAACA
    GATATTCTAC AGATGATTGG TAGAGCTGGT CGACCTCAAT TTGACACTAC AGCTACTGCA
    GTTATTATGA CTCGGTTAAG CACAAAAGAG AAGTATATTC AGATGCTTGC TTGCAGTGAC
    ACTATAGAAA GCAGTTTGCA CAGACATCTT ATTGAGCATT TAAATGCAGA GATAGTATTG
    CATACCATCA CAAACGTGAA TATTGCTTTG GAATGGATAC GATCAACCTT GCTTTATATC
    AGAGCCTTGA AAAATCCATC TCATTATGGT TTTGCATCTG GATTGAACAA AGATGGAATT
    GAATCAAAAT TACAAGAATT GTGTTTGAAG AATCTGAATG ATTTGTCATC TCTGAACCTG
    ATAAAAATGG ATGAAGATAT TAATTTCAAA CCAACTGAGG CAGGAAGATT GATGGCTTGG
    TATTATATTA CATTTGAGAC AGTGAAGAAA TTTTGTACAA TCAGTGGAAA AGAAACTTTA
    TCAGATCTGG TCACAATGAT AGCCAGCTGC AAGGAATTTC TAGATGTACA ATTAAGAATA
    AATGAAAAGA AAGCATTGAA TACTTTGAAC AAAGATCCAA ATCGGACAAC TATCAGATTT
    CCAATGGAAG GAAGGATTAA AACAAGAGAA ATGAAAGTAA ATTGTCTTAT TCAGGCTCAG
    CTAGGATGCA TTCCCGTACA AGACTTTGCT TTGACGCAAG ATACTGCAAA GATTTTCAGA
    AATGGCTCAC GAATTACAAG ATGGTTATCA GATTTTGTGG CTACTCAAGA AAAGTTTGCT
    GTATTATTGA ACAGTTTGAT TTTAGCTAAA TGTTTTAGAT GTAAACTTTG GGAAAACTCT
    CCACATGTAT CCAAACAGCT GGAAAAAATT GGTATAACAT TATCAAATGC TATGGTAAAT
    GCAGGTTTGA CTTCCTTTAA AAAAATAGAA GAAACAGATG CAAGGGAACT TGAGCTGATT
    TTAAATAGAC ATCCACCTTT TGGAACTCAG ATAAAAGAAA CAGTGATGTA TCTACCAAAA
    TATGAACTTG AAGTGGAACA GATTACAAGA TACAGTGATA CCATGGCAGA AATATTAGTG
    ACTGTTATAT TAAGAAACTT TGAACAGCTA CAAACTAAAA GAACAGCATC AAATTCTCAC
    TATGTCACCT TAATCATAGG TGATGCAGAT AATCAACTGG TTTTTAAGCA CAAAATTATG
    GATTCTGTTT TGCTAAAAGC TGGAAATTGG GCTAAAAAGT TTGATGTGAG GAGAGCTCTT
    AAATCTGAAG ATCTTAGCAT AAATCTAATC AGTTCAGAAT ATGTTGGACT TGATATACAG
    CGCAAATTTA CAGTCTTTTA CTCAGGACCC AAGAGATTTG GAAATCAAAT AATTATACAA
    AAAAAATCAG AAACAGAGAT TTCCCATTCT GAACATTCAG ATAGATCTAC TGTAGCAGGG
    CCCAAAAAAG GAATGACTGC TTGCAAAAAA CCTGGGAACC GAGAATGCAA TCATCACTGT
    AAAAATAAAC ATACATGTGG ACATGACTGC TGTAAAATTG GAGTTGCACA GAAGTCAGAA
    GTGAAAGAAT CAACAATTTC TTCATATTTA TCTGATTTAA GAAATAGAAA TGCTGTTTCA
    TCTCTTCCTC CAGTTAAACG TCTGAAGATG CAGATGAACA AATCTCCAAA TGTGGACCTT
    AAGGACTTTG GTTTTGCTCC AAAACCTTCT CTTCCCAGTA TAGCAAGGTC AGAATATTTA
    AACACACCTG AATTGCCTGT AATGGAGCAG AGGGATCAGC ATGAGATCTA TACAAAAAGT
    CAACAACAAC AATCATCTGC ATATCAAGAC ACAGGTAAAT CTGCATATCA AGACAAAGAA
    GTTTTGAAAG TGAACTTTGA ATTGGGAAAT GAAGTTTGGG ATGACTTTGA TGATGAAAAC
    TTAGTAGAAA TTACTAGCTT TTCAGCTGAA ACTGAGAAGA CAAAAACATC AGGATTTGGA
    AACACTTTGA GTTCAAGTAC TGGGGAAAGT AAGCTACCAT TCCAAGAATC AAAAGGCCAA
    TTCCAAAGAG AATTGTCTAA GAGTTTTGTT TCATCACATG AAAAGCCAGA TATTTATTTA
    TCAAATTCTG CTGTGTCCAA GTTTGGTGCA TCCTCCATGA CACAATTACC TCAACAATCT
    GGAAATGCAA GTATTATAAG AAGAAACACT TCTCTCTTTT TTCTGTTAAA TACTGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68416
    Clone ID Related Accession (Same CDS sequence) XM_025288299.1
    Accession Version XM_025288299.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product probable ATP-dependent DNA helicase HFM1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AGTTATTTAA AATATCTGAT AATATACATG AGGATGCTTA TTCCAATGAC
    AGTGCAAACT TGGACTCTAA TATTGGCTCA GTGAAAATTG TCCAAACAGA AGTGAACAAA
    GGGAAATCAT GGAAAGATAG CAATAGTAAG CAGAAACCTC AATATTCAGA CTGTAACATT
    TTGACAGCCA CTGATACACT TTTTGCTTCT GAAATCGGAG AAGGCATAAT CAAAGCACCA
    CCTTTTCCAG TTGCATCCCA GCCTCATACA GTTCAAGGGG TAACAGAAAG AAGTTTAGAT
    TCCTTGAAGG CTGTCACAGA AATTCCTGCT AAATTTAGAA GTATTTTCAA AGAATTCCCA
    TATTTCAATT ATATACAGTC CAAAGCCTTT GATGATCTTC TTTATACAGA TAGGAATTTT
    GTGATTTGTG CTCCAACTGG TTCTGGGAAA ACTGTAGTGT TTGAACTAGC TATAACAAGA
    TTGTTAATGG AAGTACCATT GCCATGGTCA AACATTAAAA TTGTTTACAT GGCACCAATA
    AAAGCCCTGT GTAGTCAGCG TTTTGATGAT TGGAAAAAAA AATTTGGACC AATAGGATTG
    AATTGTAAAG AACTCACTGG AGATACAGTA ATGGATGACC TATTTGAGAT TCAGCATGCC
    CATATTATTA TGACAACTCC AGAAAAATGG GATAGCATGA CTAGAAAATG GAGAGACAAC
    TCTTTGGTCC ATCTGGTTCG ATTGTTTCTC ATTGATGAGG TACATGTTGT AAAAGATGAA
    AATCGTGGAC CAACTCTTGA AGTTGTAGTC AGCAGAATGA AAACTGTGCA GATTTTATCT
    CATGCTGTAG AAAATACCAA CAGTATTATT CCAATGAGAT TTGTGGCTGT ATCTGCAACA
    ATTCCAAATG CTGAGGACAT TGCAGAATGG CTTTCAGATG GTGAAAGATC TGCTGTATGT
    CTGAAAATGG ATGAGAGACA TAGACCAGTG AAACTTCGGA AAGTGGTTCT TGGGTTTCCC
    TGCAGTGATA ACCAAACTGA ATTCAAGTTT GATTTAACGC TCAACTACAA AATTGCCAGT
    ATTATACAAA CGTACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCGGT TCTTGTGAAA GATGCAAAAT TTATTATGAC TGTGGAACAG
    AAACAGAGGT TACAAAAGTG TGCATATTCC ATAAAAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAACTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTTA CTGTTGGAGA TGTACCAGTT CTTTTTACTA CCAGTACCTT AGCCATGGGA
    GTCAATTTGC CTGCTCACCT AGTGGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ACAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGACACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GCACAAAAGA GAAGTATATT
    CAGATGCTTG CTTGCAGTGA CACTATAGAA AGCAGTTTGC ACAGACATCT TATTGAGCAT
    TTAAATGCAG AGATAGTATT GCATACCATC ACAAACGTGA ATATTGCTTT GGAATGGATA
    CGATCAACCT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAATCAAAA TTACAAGAAT TGTGTTTGAA GAATCTGAAT
    GATTTGTCAT CTCTGAACCT GATAAAAATG GATGAAGATA TTAATTTCAA ACCAACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTATATT ACATTTGAGA CAGTGAAGAA ATTTTGTACA
    ATCAGTGGAA AAGAAACTTT ATCAGATCTG GTCACAATGA TAGCCAGCTG CAAGGAATTT
    CTAGATGTAC AATTAAGAAT AAATGAAAAG AAAGCATTGA ATACTTTGAA CAAAGATCCA
    AATCGGACAA CTATCAGATT TCCAATGGAA GGAAGGATTA AAACAAGAGA AATGAAAGTA
    AATTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCCGTAC AAGACTTTGC TTTGACGCAA
    GATACTGCAA AGATTTTCAG AAATGGCTCA CGAATTACAA GATGGTTATC AGATTTTGTG
    GCTACTCAAG AAAAGTTTGC TGTATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGTAAACTTT GGGAAAACTC TCCACATGTA TCCAAACAGC TGGAAAAAAT TGGTATAACA
    TTATCAAATG CTATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAGCTGAT TTTAAATAGA CATCCACCTT TTGGAACTCA GATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAACTT GAAGTGGAAC AGATTACAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACTGTTATA TTAAGAAACT TTGAACAGCT ACAAACTAAA
    AGAACAGCAT CAAATTCTCA CTATGTCACC TTAATCATAG GTGATGCAGA TAATCAACTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTGCTAAAAG CTGGAAATTG GGCTAAAAAG
    TTTGATGTGA GGAGAGCTCT TAAATCTGAA GATCTTAGCA TAAATCTAAT CAGTTCAGAA
    TATGTTGGAC TTGATATACA GCGCAAATTT ACAGTCTTTT ACTCAGGACC CAAGAGATTT
    GGAAATCAAA TAATTATACA AAAAAAATCA GAAACAGAGA TTTCCCATTC TGAACATTCA
    GATAGATCTA CTGTAGCAGG GCCCAAAAAA GGAATGACTG CTTGCAAAAA ACCTGGGAAC
    CGAGAATGCA ATCATCACTG TAAAAATAAA CATACATGTG GACATGACTG CTGTAAAATT
    GGAGTTGCAC AGAAGTCAGA AGTGAAAGAA TCAACAATTT CTTCATATTT ATCTGATTTA
    AGAAATAGAA ATGCTGTTTC ATCTCTTCCT CCAGTTAAAC GTCTGAAGAT GCAGATGAAC
    AAATCTCCAA ATGTGGACCT TAAGGACTTT GGTTTTGCTC CAAAACCTTC TCTTCCCAGT
    ATAGCAAGGT CAGAATATTT AAACACACCT GAATTGCCTG TAATGGAGCA GAGGGATCAG
    CATGAGATCT ATACAAAAAG TCAACAACAA CAATCATCTG CATATCAAGA CACAGGTAAA
    TCTGCATATC AAGACAAAGA AGTTTTGAAA GTGAACTTTG AATTGGGAAA TGAAGTTTGG
    GATGACTTTG ATGATGAAAA CTTAGTAGAA ATTACTAGCT TTTCAGCTGA AACTGAGAAG
    ACAAAAACAT CAGGATTTGG AAACACTTTG AGTTCAAGTA CTGGGGAAAG TAAGCTACCA
    TTCCAAGAAT CAAAAGGCCA ATTCCAAAGA GAATTGTCTA AGAGTTTTGT TTCATCACAT
    GAAAAGCCAG ATATTTATTT ATCAAATTCT GCTGTGTCCA AGTTTGGTGC ATCCTCCATG
    ACACAATTAC CTCAACAATC TGGAAATGCA AGTATTATAA GAAGAAACAC TTCTCTCTTT
    TTTCTGTTAA ATACTGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025144084.1
    CDS1898..5638
    Translation

    Target ORF information:

    RefSeq Version XM_025288299.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025288299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA AGTTATTTAA AATATCTGAT AATATACATG AGGATGCTTA TTCCAATGAC
    AGTGCAAACT TGGACTCTAA TATTGGCTCA GTGAAAATTG TCCAAACAGA AGTGAACAAA
    GGGAAATCAT GGAAAGATAG CAATAGTAAG CAGAAACCTC AATATTCAGA CTGTAACATT
    TTGACAGCCA CTGATACACT TTTTGCTTCT GAAATCGGAG AAGGCATAAT CAAAGCACCA
    CCTTTTCCAG TTGCATCCCA GCCTCATACA GTTCAAGGGG TAACAGAAAG AAGTTTAGAT
    TCCTTGAAGG CTGTCACAGA AATTCCTGCT AAATTTAGAA GTATTTTCAA AGAATTCCCA
    TATTTCAATT ATATACAGTC CAAAGCCTTT GATGATCTTC TTTATACAGA TAGGAATTTT
    GTGATTTGTG CTCCAACTGG TTCTGGGAAA ACTGTAGTGT TTGAACTAGC TATAACAAGA
    TTGTTAATGG AAGTACCATT GCCATGGTCA AACATTAAAA TTGTTTACAT GGCACCAATA
    AAAGCCCTGT GTAGTCAGCG TTTTGATGAT TGGAAAAAAA AATTTGGACC AATAGGATTG
    AATTGTAAAG AACTCACTGG AGATACAGTA ATGGATGACC TATTTGAGAT TCAGCATGCC
    CATATTATTA TGACAACTCC AGAAAAATGG GATAGCATGA CTAGAAAATG GAGAGACAAC
    TCTTTGGTCC ATCTGGTTCG ATTGTTTCTC ATTGATGAGG TACATGTTGT AAAAGATGAA
    AATCGTGGAC CAACTCTTGA AGTTGTAGTC AGCAGAATGA AAACTGTGCA GATTTTATCT
    CATGCTGTAG AAAATACCAA CAGTATTATT CCAATGAGAT TTGTGGCTGT ATCTGCAACA
    ATTCCAAATG CTGAGGACAT TGCAGAATGG CTTTCAGATG GTGAAAGATC TGCTGTATGT
    CTGAAAATGG ATGAGAGACA TAGACCAGTG AAACTTCGGA AAGTGGTTCT TGGGTTTCCC
    TGCAGTGATA ACCAAACTGA ATTCAAGTTT GATTTAACGC TCAACTACAA AATTGCCAGT
    ATTATACAAA CGTACTCTGA TCAGAAACCC ACACTTGTGT TTTGTGCAAC AAGGAAAGGT
    GTGCAACAGG CTGCTTCGGT TCTTGTGAAA GATGCAAAAT TTATTATGAC TGTGGAACAG
    AAACAGAGGT TACAAAAGTG TGCATATTCC ATAAAAGATT CAAAACTGAG AGATATCTTA
    ATACATGGTG TTGCTTATCA TCATGCTGGT ATGGAACTGT CAGATAGAAA AGTAGTTGAA
    GGAGCTTTTA CTGTTGGAGA TGTACCAGTT CTTTTTACTA CCAGTACCTT AGCCATGGGA
    GTCAATTTGC CTGCTCACCT AGTGGTTATA AAATCTACAA TGCATTATGC TGGAGGAATG
    TTTGAAGAAT ACAGTGAAAC AGATATTCTA CAGATGATTG GTAGAGCTGG TCGACCTCAA
    TTTGACACTA CAGCTACTGC AGTTATTATG ACTCGGTTAA GCACAAAAGA GAAGTATATT
    CAGATGCTTG CTTGCAGTGA CACTATAGAA AGCAGTTTGC ACAGACATCT TATTGAGCAT
    TTAAATGCAG AGATAGTATT GCATACCATC ACAAACGTGA ATATTGCTTT GGAATGGATA
    CGATCAACCT TGCTTTATAT CAGAGCCTTG AAAAATCCAT CTCATTATGG TTTTGCATCT
    GGATTGAACA AAGATGGAAT TGAATCAAAA TTACAAGAAT TGTGTTTGAA GAATCTGAAT
    GATTTGTCAT CTCTGAACCT GATAAAAATG GATGAAGATA TTAATTTCAA ACCAACTGAG
    GCAGGAAGAT TGATGGCTTG GTATTATATT ACATTTGAGA CAGTGAAGAA ATTTTGTACA
    ATCAGTGGAA AAGAAACTTT ATCAGATCTG GTCACAATGA TAGCCAGCTG CAAGGAATTT
    CTAGATGTAC AATTAAGAAT AAATGAAAAG AAAGCATTGA ATACTTTGAA CAAAGATCCA
    AATCGGACAA CTATCAGATT TCCAATGGAA GGAAGGATTA AAACAAGAGA AATGAAAGTA
    AATTGTCTTA TTCAGGCTCA GCTAGGATGC ATTCCCGTAC AAGACTTTGC TTTGACGCAA
    GATACTGCAA AGATTTTCAG AAATGGCTCA CGAATTACAA GATGGTTATC AGATTTTGTG
    GCTACTCAAG AAAAGTTTGC TGTATTATTG AACAGTTTGA TTTTAGCTAA ATGTTTTAGA
    TGTAAACTTT GGGAAAACTC TCCACATGTA TCCAAACAGC TGGAAAAAAT TGGTATAACA
    TTATCAAATG CTATGGTAAA TGCAGGTTTG ACTTCCTTTA AAAAAATAGA AGAAACAGAT
    GCAAGGGAAC TTGAGCTGAT TTTAAATAGA CATCCACCTT TTGGAACTCA GATAAAAGAA
    ACAGTGATGT ATCTACCAAA ATATGAACTT GAAGTGGAAC AGATTACAAG ATACAGTGAT
    ACCATGGCAG AAATATTAGT GACTGTTATA TTAAGAAACT TTGAACAGCT ACAAACTAAA
    AGAACAGCAT CAAATTCTCA CTATGTCACC TTAATCATAG GTGATGCAGA TAATCAACTG
    GTTTTTAAGC ACAAAATTAT GGATTCTGTT TTGCTAAAAG CTGGAAATTG GGCTAAAAAG
    TTTGATGTGA GGAGAGCTCT TAAATCTGAA GATCTTAGCA TAAATCTAAT CAGTTCAGAA
    TATGTTGGAC TTGATATACA GCGCAAATTT ACAGTCTTTT ACTCAGGACC CAAGAGATTT
    GGAAATCAAA TAATTATACA AAAAAAATCA GAAACAGAGA TTTCCCATTC TGAACATTCA
    GATAGATCTA CTGTAGCAGG GCCCAAAAAA GGAATGACTG CTTGCAAAAA ACCTGGGAAC
    CGAGAATGCA ATCATCACTG TAAAAATAAA CATACATGTG GACATGACTG CTGTAAAATT
    GGAGTTGCAC AGAAGTCAGA AGTGAAAGAA TCAACAATTT CTTCATATTT ATCTGATTTA
    AGAAATAGAA ATGCTGTTTC ATCTCTTCCT CCAGTTAAAC GTCTGAAGAT GCAGATGAAC
    AAATCTCCAA ATGTGGACCT TAAGGACTTT GGTTTTGCTC CAAAACCTTC TCTTCCCAGT
    ATAGCAAGGT CAGAATATTT AAACACACCT GAATTGCCTG TAATGGAGCA GAGGGATCAG
    CATGAGATCT ATACAAAAAG TCAACAACAA CAATCATCTG CATATCAAGA CACAGGTAAA
    TCTGCATATC AAGACAAAGA AGTTTTGAAA GTGAACTTTG AATTGGGAAA TGAAGTTTGG
    GATGACTTTG ATGATGAAAA CTTAGTAGAA ATTACTAGCT TTTCAGCTGA AACTGAGAAG
    ACAAAAACAT CAGGATTTGG AAACACTTTG AGTTCAAGTA CTGGGGAAAG TAAGCTACCA
    TTCCAAGAAT CAAAAGGCCA ATTCCAAAGA GAATTGTCTA AGAGTTTTGT TTCATCACAT
    GAAAAGCCAG ATATTTATTT ATCAAATTCT GCTGTGTCCA AGTTTGGTGC ATCCTCCATG
    ACACAATTAC CTCAACAATC TGGAAATGCA AGTATTATAA GAAGAAACAC TTCTCTCTTT
    TTTCTGTTAA ATACTGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu68417
    Clone ID Related Accession (Same CDS sequence) XM_025288300.1
    Accession Version XM_025288300.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product probable ATP-dependent DNA helicase HFM1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037550.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCATGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA CATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCTGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CGCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATGTACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCTAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACTG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCTGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCAC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATACAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTAATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAGATCTTA GCATAAATCT AATCAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGGCCCAAA AAAGATGCAG ATGAACAAAT CTCCAAATGT
    GGACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025144085.1
    CDS1897..5325
    Translation

    Target ORF information:

    RefSeq Version XM_025288300.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HFM1, ATP dependent DNA helicase homolog (HFM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025288300.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTTGGAAT CAAATGACTC TGTGTTTTCC TTGGATAATT TGTTTTTTGA AAAAACAGAT 
    GAAGCTGAAA ACCACCTAGA CAGTGAAAAA TCTTTGGATT GGTTTCTTCC ACCTGCTCCA
    CTGATTTCAG AAATTCCAGA TCCTCAGGCA TTAGAGGAAG AAATAGAAAA TCGTAAACTG
    TTAGGTCAGA ATAAAAGATC AAAAATGTTA CCATCAAATT TAAAGATAAC TAATGAAGAC
    ACAAATTATG TTTCACAAAC CCAAAAATTC CAGTTTAACT TCCCTTCTAA TGAATATGAA
    GAAGATGATC TAAATTTAGG AGGGGCAGAT AACAATGACT TACATGTTGC TGGCAAGCTG
    ACATGTGGTT CTTCTCAGAA ATGCAGAAAT CACATTGGCA CTGAGATAGA ACTTGAGAAA
    AATGTTCCAG ATGATACAAA GTTAGTTAAT TCTGCACAAG ATAAAGGAGT GAGCATGTCA
    GCCTTCCGCA AAAGGTTATT TAAAATATCT GATAATATAC ATGAGGATGC TTATTCCAAT
    GACAGTGCAA ACTTGGACTC TAATATTGGC TCAGTGAAAA TTGTCCAAAC AGAAGTGAAC
    AAAGGGAAAT CATGGAAAGA TAGCAATAGT AAGCAGAAAC CTCAATATTC AGACTGTAAC
    ATTTTGACAG CCACTGATAC ACTTTTTGCT TCTGAAATCG GAGAAGGCAT AATCAAAGCA
    CCACCTTTTC CAGTTGCATC CCAGCCTCAT ACAGTTCAAG GGGTAACAGA AAGAAGTTTA
    GATTCCTTGA AGGCTGTCAC AGAAATTCCT GCTAAATTTA GAAGTATTTT CAAAGAATTC
    CCATATTTCA ATTATATACA GTCCAAAGCC TTTGATGATC TTCTTTATAC AGATAGGAAT
    TTTGTGATTT GTGCTCCAAC TGGTTCTGGG AAAACTGTAG TGTTTGAACT AGCTATAACA
    AGATTGTTAA TGGAAGTACC ATTGCCATGG TCAAACATTA AAATTGTTTA CATGGCACCA
    ATAAAAGCCC TGTGTAGTCA GCGTTTTGAT GATTGGAAAA AAAAATTTGG ACCAATAGGA
    TTGAATTGTA AAGAACTCAC TGGAGATACA GTAATGGATG ACCTATTTGA GATTCAGCAT
    GCCCATATTA TTATGACAAC TCCAGAAAAA TGGGATAGCA TGACTAGAAA ATGGAGAGAC
    AACTCTTTGG TCCATCTGGT TCGATTGTTT CTCATTGATG AGGTACATGT TGTAAAAGAT
    GAAAATCGTG GACCAACTCT TGAAGTTGTA GTCAGCAGAA TGAAAACTGT GCAGATTTTA
    TCTCATGCTG TAGAAAATAC CAACAGTATT ATTCCAATGA GATTTGTGGC TGTATCTGCA
    ACAATTCCAA ATGCTGAGGA CATTGCAGAA TGGCTTTCAG ATGGTGAAAG ATCTGCTGTA
    TGTCTGAAAA TGGATGAGAG ACATAGACCA GTGAAACTTC GGAAAGTGGT TCTTGGGTTT
    CCCTGCAGTG ATAACCAAAC TGAATTCAAG TTTGATTTAA CGCTCAACTA CAAAATTGCC
    AGTATTATAC AAACGTACTC TGATCAGAAA CCCACACTTG TGTTTTGTGC AACAAGGAAA
    GGTGTGCAAC AGGCTGCTTC GGTTCTTGTG AAAGATGCAA AATTTATTAT GACTGTGGAA
    CAGAAACAGA GGTTACAAAA GTGTGCATAT TCCATAAAAG ATTCAAAACT GAGAGATATC
    TTAATACATG GTGTTGCTTA TCATCATGCT GGTATGGAAC TGTCAGATAG AAAAGTAGTT
    GAAGGAGCTT TTACTGTTGG AGATGTACCA GTTCTTTTTA CTACCAGTAC CTTAGCCATG
    GGAGTCAATT TGCCTGCTCA CCTAGTGGTT ATAAAATCTA CAATGCATTA TGCTGGAGGA
    ATGTTTGAAG AATACAGTGA AACAGATATT CTACAGATGA TTGGTAGAGC TGGTCGACCT
    CAATTTGACA CTACAGCTAC TGCAGTTATT ATGACTCGGT TAAGCACAAA AGAGAAGTAT
    ATTCAGATGC TTGCTTGCAG TGACACTATA GAAAGCAGTT TGCACAGACA TCTTATTGAG
    CATTTAAATG CAGAGATAGT ATTGCATACC ATCACAAACG TGAATATTGC TTTGGAATGG
    ATACGATCAA CCTTGCTTTA TATCAGAGCC TTGAAAAATC CATCTCATTA TGGTTTTGCA
    TCTGGATTGA ACAAAGATGG AATTGAATCA AAATTACAAG AATTGTGTTT GAAGAATCTG
    AATGATTTGT CATCTCTGAA CCTGATAAAA ATGGATGAAG ATATTAATTT CAAACCAACT
    GAGGCAGGAA GATTGATGGC TTGGTATTAT ATTACATTTG AGACAGTGAA GAAATTTTGT
    ACAATCAGTG GAAAAGAAAC TTTATCAGAT CTGGTCACAA TGATAGCCAG CTGCAAGGAA
    TTTCTAGATG TACAATTAAG AATAAATGAA AAGAAAGCAT TGAATACTTT GAACAAAGAT
    CCAAATCGGA CAACTATCAG ATTTCCAATG GAAGGAAGGA TTAAAACAAG AGAAATGAAA
    GTAAATTGTC TTATTCAGGC TCAGCTAGGA TGCATTCCCG TACAAGACTT TGCTTTGACG
    CAAGATACTG CAAAGATTTT CAGAAATGGC TCACGAATTA CAAGATGGTT ATCAGATTTT
    GTGGCTACTC AAGAAAAGTT TGCTGTATTA TTGAACAGTT TGATTTTAGC TAAATGTTTT
    AGATGTAAAC TTTGGGAAAA CTCTCCACAT GTATCCAAAC AGCTGGAAAA AATTGGTATA
    ACATTATCAA ATGCTATGGT AAATGCAGGT TTGACTTCCT TTAAAAAAAT AGAAGAAACA
    GATGCAAGGG AACTTGAGCT GATTTTAAAT AGACATCCAC CTTTTGGAAC TCAGATAAAA
    GAAACAGTGA TGTATCTACC AAAATATGAA CTTGAAGTGG AACAGATTAC AAGATACAGT
    GATACCATGG CAGAAATATT AGTGACTGTT ATATTAAGAA ACTTTGAACA GCTACAAACT
    AAAAGAACAG CATCAAATTC TCACTATGTC ACCTTAATCA TAGGTGATGC AGATAATCAA
    CTGGTTTTTA AGCACAAAAT TATGGATTCT GTTTTGCTAA AAGCTGGAAA TTGGGCTAAA
    AAGTTTGATG TGAGGAGAGC TCTTAAATCT GAAGATCTTA GCATAAATCT AATCAGTTCA
    GAATATGTTG GACTTGATAT ACAGCGCAAA TTTACAGTCT TTTACTCAGG ACCCAAGAGA
    TTTGGAAATC AAATAATTAT ACAAAAAAAA TCAGAAACAG AGATTTCCCA TTCTGAACAT
    TCAGATAGAT CTACTGTAGC AGGGCCCAAA AAAGATGCAG ATGAACAAAT CTCCAAATGT
    GGACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.